LOCUS       DASJST010000001        55938 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJST010000001 DASJST010000000
VERSION     DASJST010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.001"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..459
                     /locus_tag="U4B05_000001"
     CDS             19..459
                     /locus_tag="U4B05_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2049172.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            587..1270
                     /locus_tag="U4B05_000002"
     CDS             587..1270
                     /locus_tag="U4B05_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN2049173.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1579..1652
                     /locus_tag="U4B05_000003"
     tRNA            1579..1652
                     /locus_tag="U4B05_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1613..1615,aa:Arg,seq:tct)
     gene            1811..2170
                     /locus_tag="U4B05_000004"
     CDS             1811..2170
                     /locus_tag="U4B05_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN2049174.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2293..3924
                     /locus_tag="U4B05_000005"
     CDS             2293..3924
                     /locus_tag="U4B05_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN2049175.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3986..4627)
                     /locus_tag="U4B05_000006"
     CDS             complement(3986..4627)
                     /locus_tag="U4B05_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2049176.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4813..4889
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4909..5217
                     /gene="rplU"
                     /locus_tag="U4B05_000007"
     CDS             4909..5217
                     /gene="rplU"
                     /locus_tag="U4B05_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN2049177.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5244..5588
                     /locus_tag="U4B05_000008"
     CDS             5244..5588
                     /locus_tag="U4B05_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN2049178.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5601..5894
                     /gene="rpmA"
                     /locus_tag="U4B05_000009"
     CDS             5601..5894
                     /gene="rpmA"
                     /locus_tag="U4B05_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN2049179.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6328..9135
                     /locus_tag="U4B05_000010"
     CDS             6328..9135
                     /locus_tag="U4B05_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2049180.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9326..9817
                     /locus_tag="U4B05_000011"
     CDS             9326..9817
                     /locus_tag="U4B05_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2049181.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9920..10909
                     /gene="manX"
                     /locus_tag="U4B05_000012"
     CDS             9920..10909
                     /gene="manX"
                     /locus_tag="U4B05_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN2049182.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10942..11745
                     /locus_tag="U4B05_000013"
     CDS             10942..11745
                     /locus_tag="U4B05_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN2049183.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11766..12680
                     /locus_tag="U4B05_000014"
     CDS             11766..12680
                     /locus_tag="U4B05_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN2049184.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12763..13137
                     /locus_tag="U4B05_000015"
     CDS             12763..13137
                     /locus_tag="U4B05_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN2049185.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13276..13521
                     /locus_tag="U4B05_000016"
     CDS             13276..13521
                     /locus_tag="U4B05_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2049186.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13693..14757
                     /locus_tag="U4B05_000017"
     CDS             13693..14757
                     /locus_tag="U4B05_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2049187.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15003..15428
                     /locus_tag="U4B05_000018"
     CDS             15003..15428
                     /locus_tag="U4B05_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2049188.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15421..15870
                     /gene="nusB"
                     /locus_tag="U4B05_000019"
     CDS             15421..15870
                     /gene="nusB"
                     /locus_tag="U4B05_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN2049189.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15967..16821
                     /locus_tag="U4B05_000020"
     CDS             15967..16821
                     /locus_tag="U4B05_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN2049190.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16822..18159
                     /locus_tag="U4B05_000021"
     CDS             16822..18159
                     /locus_tag="U4B05_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN2049191.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18164..18388
                     /locus_tag="U4B05_000022"
     CDS             18164..18388
                     /locus_tag="U4B05_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN2049192.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18390..19280
                     /locus_tag="U4B05_000023"
     CDS             18390..19280
                     /locus_tag="U4B05_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2049193.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19289..20107
                     /locus_tag="U4B05_000024"
     CDS             19289..20107
                     /locus_tag="U4B05_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN2049194.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20214..20663
                     /locus_tag="U4B05_000025"
     CDS             20214..20663
                     /locus_tag="U4B05_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2049195.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20681..22363
                     /gene="recN"
                     /locus_tag="U4B05_000026"
     CDS             20681..22363
                     /gene="recN"
                     /locus_tag="U4B05_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN2049196.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22418..22534)
                     /locus_tag="U4B05_000027"
     CDS             complement(22418..22534)
                     /locus_tag="U4B05_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN2049197.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22607..23563)
                     /locus_tag="U4B05_000028"
     CDS             complement(22607..23563)
                     /locus_tag="U4B05_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2049198.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23734..24102
                     /locus_tag="U4B05_000029"
     CDS             23734..24102
                     /locus_tag="U4B05_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN2049199.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24272..24703
                     /gene="mraZ"
                     /locus_tag="U4B05_000030"
     CDS             24272..24703
                     /gene="mraZ"
                     /locus_tag="U4B05_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN2049200.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24724..25683
                     /gene="rsmH"
                     /locus_tag="U4B05_000031"
     CDS             24724..25683
                     /gene="rsmH"
                     /locus_tag="U4B05_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN2049201.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25688..26089
                     /gene="ftsL"
                     /locus_tag="U4B05_000032"
     CDS             25688..26089
                     /gene="ftsL"
                     /locus_tag="U4B05_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN2049202.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26090..28282
                     /locus_tag="U4B05_000033"
     CDS             26090..28282
                     /locus_tag="U4B05_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2049203.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28308..29270
                     /locus_tag="U4B05_000034"
     CDS             28308..29270
                     /locus_tag="U4B05_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN2049204.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29284..30645
                     /locus_tag="U4B05_000035"
     CDS             29284..30645
                     /locus_tag="U4B05_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN2049205.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30671..31759
                     /gene="murG"
                     /locus_tag="U4B05_000036"
     CDS             30671..31759
                     /gene="murG"
                     /locus_tag="U4B05_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN2049206.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31774..32994
                     /locus_tag="U4B05_000037"
     CDS             31774..32994
                     /locus_tag="U4B05_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN2049207.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33150..34475
                     /gene="ftsA"
                     /locus_tag="U4B05_000038"
     CDS             33150..34475
                     /gene="ftsA"
                     /locus_tag="U4B05_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN2049208.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34498..35739
                     /gene="ftsZ"
                     /locus_tag="U4B05_000039"
     CDS             34498..35739
                     /gene="ftsZ"
                     /locus_tag="U4B05_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN2049209.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35756..36433
                     /locus_tag="U4B05_000040"
     CDS             35756..36433
                     /locus_tag="U4B05_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN2049210.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36447..37046
                     /locus_tag="U4B05_000041"
     CDS             36447..37046
                     /locus_tag="U4B05_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN2049211.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37061..37348
                     /locus_tag="U4B05_000042"
     CDS             37061..37348
                     /locus_tag="U4B05_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN2049212.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37397..38179
                     /locus_tag="U4B05_000043"
     CDS             37397..38179
                     /locus_tag="U4B05_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN2049213.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38200..38907
                     /locus_tag="U4B05_000044"
     CDS             38200..38907
                     /locus_tag="U4B05_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN2049214.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39169..41967
                     /gene="ileS"
                     /locus_tag="U4B05_000045"
     CDS             39169..41967
                     /gene="ileS"
                     /locus_tag="U4B05_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN2049215.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42016..43542)
                     /locus_tag="U4B05_000046"
     CDS             complement(42016..43542)
                     /locus_tag="U4B05_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN2049216.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43557..44204)
                     /locus_tag="U4B05_000047"
     CDS             complement(43557..44204)
                     /locus_tag="U4B05_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN2049217.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44388..44717)
                     /locus_tag="U4B05_000048"
     CDS             complement(44388..44717)
                     /locus_tag="U4B05_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN2049218.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44894..45622
                     /gene="sfsA"
                     /locus_tag="U4B05_000049"
     CDS             44894..45622
                     /gene="sfsA"
                     /locus_tag="U4B05_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN2049219.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45638..46651
                     /locus_tag="U4B05_000050"
     CDS             45638..46651
                     /locus_tag="U4B05_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2049220.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46651..47928
                     /locus_tag="U4B05_000051"
     CDS             46651..47928
                     /locus_tag="U4B05_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2049221.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47991..50693
                     /locus_tag="U4B05_000052"
     CDS             47991..50693
                     /locus_tag="U4B05_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2049222.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50845..51162
                     /locus_tag="U4B05_000053"
     CDS             50845..51162
                     /locus_tag="U4B05_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2049223.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51192..51512
                     /locus_tag="U4B05_000054"
     CDS             51192..51512
                     /locus_tag="U4B05_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2049224.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51620..53080
                     /locus_tag="U4B05_000055"
     CDS             51620..53080
                     /locus_tag="U4B05_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2049225.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53148..53369
                     /locus_tag="U4B05_000056"
     CDS             53148..53369
                     /locus_tag="U4B05_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049226.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53400..53582
                     /locus_tag="U4B05_000057"
     CDS             53400..53582
                     /locus_tag="U4B05_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN2049227.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53582..53995
                     /locus_tag="U4B05_000058"
     CDS             53582..53995
                     /locus_tag="U4B05_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN2049228.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54118..55299
                     /locus_tag="U4B05_000059"
     CDS             54118..55299
                     /locus_tag="U4B05_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049229.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55370..55534)
                     /locus_tag="U4B05_000060"
     CDS             complement(55370..55534)
                     /locus_tag="U4B05_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049230.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55688..55761
                     /locus_tag="U4B05_000061"
     tRNA            55688..55761
                     /locus_tag="U4B05_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55722..55724,aa:Arg,seq:cct)
BASE COUNT        18475 a         9458 c        12067 g        15938 t
ORIGIN      
        1 cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta tattattgag
       61 gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg accgaaaaat
      121 acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg aaaaaaaatc
      181 tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac aaaagctgat
      241 acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg tagtcccatc
      301 tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg gattcagggg
      361 ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac ctttgtagag
      421 ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa acataaatgt
      481 attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc atgcagcttg
      541 tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc gcaatgaaat
      601 gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc agcaaaaaaa
      661 attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag taccagttat
      721 tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc ctgaacgaat
      781 cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga caatcggaga
      841 aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag ctaaaaaaaa
      901 ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag cagcagatat
      961 cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa aatcaaaata
     1021 tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt tgatggtaga
     1081 tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc gtggcaaacg
     1141 agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc ccgatttgac
     1201 atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat cagaaacaat
     1261 cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga gggctcaatt
     1321 cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag gaaatctaaa
     1381 taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac cttagaacag
     1441 ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa ataattcatt
     1501 atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc tataattatt
     1561 gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc cttctaagcg
     1621 gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa ctgaggtttc
     1681 taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca aataaaataa
     1741 aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca tcagaaaaga
     1801 ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa ttgaattttt
     1861 ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta gggtttttaa
     1921 aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga accaaggagt
     1981 gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt ccgcatctaa
     2041 agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg gcagtttgac
     2101 tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat tgctttctat
     2161 tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt gaaaaatatt
     2221 actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt tcatgaaaaa
     2281 ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt ttcaggttat
     2341 gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc tgtagcattg
     2401 cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg tttgattacg
     2461 gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca aatatcaggt
     2521 ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg aatgcaaggt
     2581 gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact tttccgatta
     2641 ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc aggtattgct
     2701 atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga aggtacaaac
     2761 gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac agcctctttg
     2821 atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg ggcgaaacga
     2881 atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt gttccattta
     2941 ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg tttgaatatg
     3001 ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag cattgcttta
     3061 ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc aaataagccc
     3121 ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct tcctttcttt
     3181 ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa atctggtgca
     3241 caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat gcttgttttt
     3301 gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat tgtgacggct
     3361 tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata ttggtcagca
     3421 gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag tattgctatc
     3481 gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc tatcacgatt
     3541 tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa gctgaataac
     3601 tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa actcaaaaat
     3661 aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg cgtaccaagt
     3721 attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga gcaaagagga
     3781 caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc tcacttgtgg
     3841 gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc tttacatgcg
     3901 aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact gtaaactgaa
     3961 gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct agtacttgcc
     4021 agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct ggatgaaaaa
     4081 gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt tctgttaatt
     4141 gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat tctttgccaa
     4201 gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggctgg accttcatca
     4261 gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt gtccgatttg
     4321 gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg cgatacgggc
     4381 ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt gaagaaagtg
     4441 cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca atctctgttt
     4501 tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc ccagcgataa
     4561 atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt tcttttgggt
     4621 attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca caatgcgact
     4681 attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa tttttgtttt
     4741 actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct agtactgttg
     4801 taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag catatgctgt
     4861 cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat gtacgcaatt
     4921 attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta tgttgaaaaa
     4981 cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt aggcggagaa
     5041 tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac tgttgaaaaa
     5101 cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca ttcacaccgt
     5161 aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa cgcttaatag
     5221 cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat gacgcaggtc
     5281 agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga agtgatatcg
     5341 tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc tcattagccg
     5401 gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat accgaagtca
     5461 cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa aatcttttat
     5521 taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa acaattaatg
     5581 ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt gcccacaaaa
     5641 aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta ggtgctaaac
     5701 gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc ggtacaaaaa
     5761 tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca aaagttgatg
     5821 gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt tatccagtag
     5881 ctcaagaagc ttaattgaat cttatgatta aagaggttgt gacaagactt ttgtcacagc
     5941 cttttttgtt tataaaaata aaagttgtag tggaggctac gaagtttact ttgtcttttt
     6001 acacttaata tttttttgcc aatttggaat caggatgttt tgataactat cgttttcaat
     6061 tgatggaagc tatattgttt gctatcagtg aaataattat atggtaaaca gtgttctttt
     6121 tcttgtttcc attataaaaa tcgtatcggg atttttcgtt cttttaaatt gaacatttct
     6181 atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat agttgacgaa acagaaataa
     6241 atacatagta ttatgttttg aagcctaagt acaatagtct atagaaaagg agcgatacca
     6301 ttggcaaaac gcattgaccg aatttatatg tatgtcaaag aaaaaaccgc tcatttaaca
     6361 aaggcagaat atgatcaagg agtaacaaca caggaaattg ctgaagtgtt agggattcag
     6421 cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg aaggaaaact gataaagaca
     6481 gacggacgac cagtacgtta catatatcaa acactcgtta cgcacgtaaa gccagttaca
     6541 aaacatgtag tcagttacaa agaaacgccg gtctctgaag aaaaaaatac actccaaata
     6601 gatactaagg atatctttgc aaaaatcatt ggagctaacg gcagcatgaa aaattcagtg
     6661 gaacaggcaa aagctgcgat tttgtatccg ccaagaggat taaattgttt gattacagga
     6721 cctaccggat caggaaagac ttattttgca cacgcgatgt atcattttgc aaaagcgcat
     6781 catgtaatag atgaggaaac ggaactgatt gttttcaatt gtgctgatta tgccaataac
     6841 cctgagcttt tgatgagtca tttatttggg tacgcaaaag gtgcttttac aggcgcagat
     6901 gaagaaaaaa tggggattat cgatcaagcg gatggcggta tgctgttttt ggatgagatt
     6961 caccggttgc cgcctgaagg tcaagaaatg atcttttact ttatggatca tggaacttac
     7021 agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg tacggatcgt aggtgcgacc
     7081 acagaagacc caggttcttc tctattagaa acttttgttc ggcgtatccc aatcaatatc
     7141 aaacttccgg cttttgaaaa acgtcctgca aatgagaaag tcgatttagt aaaaatcatg
     7201 attgcccacg aagcaaaccg cacgcagcga aagatttctt tgacagaaga tgtagcaaaa
     7261 gcattgatcg gcagtgtgac ctatggaaat ataggacaat tgaagtcaaa tatccagtta
     7321 gtatgtgcac gaggatttct aaatcatatg caatcacccg aaatctcgat cacaatcgat
     7381 gatttgacag aagggattcg tagcggattg attcaattag caagcaaccg cagtgcaatg
     7441 gcagagcttt ctaaaatatt ggagccaaag ataactgtag caccaaatga tacgatcatg
     7501 aaaatccaat cggactccta tgagttacct tataatctat atgatatcat cggagataaa
     7561 gcagctttac tgaagtcgga tggtttggat caagaagcaa tcaatcattt catttctaca
     7621 gatatcaaca tccatctgaa gtctttctat aaagaccatg gtttttcttt taatgcagac
     7681 agcaaattag cagaatttgt agatccgaaa atcatagaag tgaccaatca gatttatgta
     7741 atggtcaaaa gatctttacc atatgagttt cagcaaaact ttatttatgc tatgagcttg
     7801 catatcagtt cttttttgaa aaaaatcaac ttaggagaag aacggcatac gaatgataac
     7861 atccgtgaga tggcttttga ttttccagat gaatatgctg ttgcgaaaga agtgcgtagt
     7921 tatatcgaaa cgtattttca agtggagatt ccagaaagtg aagactatta cttgaccgtt
     7981 ctactcgtct cgttacgggc aaatcaagca agcggcagaa tcggtgtagt cgttgcagct
     8041 cacggaaaca gcacagcaag cagtatggtc caagttgtcc aacagttgtt agatgcagag
     8101 aacgtacgag cagtagacat gcctttggat atggatccga aaacagcatt ggaccgaatt
     8161 gaacgcaatg tacaagaagt agatgaagga agcggggtca tactcttagt agatatgggg
     8221 tcccttgcgt cgtttaattc gcaaattcaa agagacactg gaattccagt acgtactgtt
     8281 gatatggtaa caacttctct agttttggaa actgtacgaa aggtctcagt attagggaca
     8341 gatctagata tgttgcatga atcattgaaa aattttcgag gttacgcaga aatatcttct
     8401 gtagaatctg ttcaggaagc aaaacctatt cataagaaaa aagctgtttt ggctatctgc
     8461 gcatctggcg aaggtacagc acaaagaatc aaagaattga tcgaacgagc ggtaaacaag
     8521 cgtcaagaaa cagaattaga agttctagct ttgtcgattg tggaagtgaa agaagaactg
     8581 ccagttatcc aaaaagaata tcagatcata gctactactg ggattactga tttgaatatc
     8641 caagccccat ttattccttt agaacgtttt attgatcaaa atatcgaagt cattttggat
     8701 cagttgctga tggaatcaga attggaggaa actgaattca tctccctaga tgaagagtca
     8761 gctaaaaata cgtgtgttga gtttatctct gataacttca tctttattaa tggtagtaag
     8821 ttaatcgatc caatgtggca attttctaca caaatctctc agacgacggg aatcggcgat
     8881 gaagaatatg gattcaaaat caacctagtg atgcacacag caggaatgat cgaacgtatt
     8941 attcggaatg aaccattgac tgtggaagaa aacgaattga caaatacaac gaatgatcct
     9001 ctttatagcc aattggcagc ctcagttgtt ctgttggaag atcagataaa agtaaaagtg
     9061 ccgatagaag aaatgtatta tctgctacgt ctagtccata atcagttaga taaaaaagaa
     9121 tacactgtgc catgaataca caaaaacgat acacttttat agtgtatcgt ttttgtgtat
     9181 tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa aagttggcac gctttttgct
     9241 tatagtagtg tgtgctagga ggtggttgtt cacacagaaa tgataggggt ttctgtgaag
     9301 tagtgaggaa gaaggagttt tgcagatgga tattcgattg gcacgtatag atgatcgttt
     9361 aatccacggg caagtcgcaa cagtttggtc gaagcaaaca ggtgttgaac gtatcattgt
     9421 gatcagcgat gtagtcgctc aagataaatt gagaaagttt ctactaaaag aagctgcacc
     9481 gccggggatc acagcaaatg taataaccgt gcagaaaatg attcaaatct atcatagtgg
     9541 attattatca cgagcaaaag tgatgctttt attcaccaat ccccaagatg ttgaagcggt
     9601 tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc ggtatgagtt ttactgaagg
     9661 caaatcaatg attaccaatt ttgtttcagt aaatcaacaa gatattgatt cctttcgtta
     9721 cttgaacagt caagaagtgg agttagagat tcgaaaagta cctgctgaca ggaaaatcct
     9781 tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa gaatggttgt tggaaaaggt
     9841 caaataattc tgttgtaatg gttagcaatt attaatattt ttgaatttgt tacaacaaaa
     9901 gtaaatagga ggtataaata tggtagggat tatcctagca agtcatggac aatttgctga
     9961 aggaatctta caatccggtt caatgatctt tggcgaacaa gaaaacgtca aagccgtgat
    10021 tttgaaacca agtgaaggtc cagatgactt gagagccaaa ttggaagaag cggttgcttc
    10081 ttttgataat caagacgaag tgttattctt agttgattta tggggcggaa caccgttcaa
    10141 tcaatcaaac acattattcg aagagcacaa agataaatgg gcaatcgtca gcggtttgaa
    10201 tttaccaatg ttgattgaag cctacgcatc acgcttttca atggaatctg ctcatgaaat
    10261 tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa gtaaaaccgg aagaattaga
    10321 accggcagaa gcaccgaaag cagcagttga agacgcacaa cctaaaggag cacttccaga
    10381 aggaactgtg gtcggagacg gaaaaatcaa atatgtatta gcacgtgttg actcacgtct
    10441 gttacatgga caagttgcga cagcttggac aaaagcagtg caaccaaacc gtattatcgt
    10501 tgtttcagat gcagtatcaa aggatgatct gcgtaagaga ctaatcgaac aagctgctcc
    10561 tccaggtgta aaagcaaatg ttattcctat tagcaaaatg atcgaagtag caaaagaccc
    10621 tcgttttgga aataccaaag cattgctttt atttgaaaat cctgaagacg tattgacagc
    10681 tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg tcaatggcgc attcggtcgg
    10741 taaagtcgtt gtaagtaaag tattgtctat gggacaagaa gacgttgaag cgtttgagaa
    10801 gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta cctaatgatt cacgcgataa
    10861 catggatgat attttgaaaa aagcaaaagc tgaattagca aaagcgtaat aaataatatt
    10921 aaaatttata ggaggcttat catgtctatt atttcaataa ttttagtcgt attagttgcc
    10981 tttctagctg gtatggaagg tattcttgat gaatttcaat ttcatcaacc actagtggca
    11041 tgtacattga tcggtttagt taccggtaac ctcgaagctg gtatcgtact tggcggtacg
    11101 cttcagatga tcgctttagg ttgggcaaat atcggtgccg ctgtagcacc agatgctgca
    11161 ttagcatcag tagcatcagc aattatttta gttttaggtg gacaaggtgt taaaggtgtt
    11221 ccttctgcga tcgcgattgc cgttcctttg gctgttgcag ggttgttctt aacaatgatc
    11281 gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg cggctgctga agaaggaaac
    11341 atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt tacaaggggt tcgtatcgcg
    11401 gtcccagcag cagcgttgct atttatccca gcacaaactg tgcaatcatt ccttgaatca
    11461 atgccagcat ggttaacaga tggtatggct atcggtgggg gaatggtcgt agctgtaggt
    11521 tacgctttgg taatcaacat gatggctaca aaagaagtat ggccattctt cgttattggt
    11581 ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc taggtgcttt aggtgttgca
    11641 ttagctctta tttacttgaa cctttctaaa atgggtggtt catcaaataa tggaggcgga
    11701 agcaactctg gtgacccttt aggcgacatt ttgaatgact attaatcttg aaggaggaaa
    11761 ataaaatggc agaagaaaaa atcaaattat caaaaaaaga tcgtttagct gttgcgtggc
    11821 gctctacctt cattcaaggt tcatggaact acgaacgtat gcaaaacggt ggctgggcat
    11881 tttctatgat tccagcaatc aaaaaattat acaaaacaaa agaagaacgt tctgcagcat
    11941 taaaacgtca cttggaattc tttaatacac acccatacat tgcttctcca attttagggg
    12001 ttacattggc tctggaagaa gaacgtgcga atggcgcgcc tgttgatgat gtagcgattc
    12061 aaggggttaa agtaggtatg atgggtcctt tagctggtgt tggtgaccca gtgttctggt
    12121 ttacagtacg tccaatgcta ggtgcactag gtgcttcatt agctatggga ggaaatatcc
    12181 ttggaccaat catcttcttc ttaggttgga acattattcg ttgggcattc atgtggtata
    12241 cacaagaatt tggctacaaa gctggttcta aaattactga tgacctttct ggcggattgt
    12301 tacaagacgt aacaaaaggt gcttcaattt tagggatgtt cgtattagct gcccttgtac
    12361 aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac tgtaaaatta gataaaggtg
    12421 cttacatcga atgggataaa ttgccttcag gtggtgaagg aatcaagagt gcatttgaac
    12481 aagttaataa tggcttagcc ctttctcctg aaaaggtaac aacattacaa gacaacttag
    12541 accaattgat tcctggttta gctgcattag gattaacatt gttatgtatg tggttgctta
    12601 agaaaaaggt ttctccaatc gttatcatcc ttggtttatt cgtagtcggt gttgttggac
    12661 acgtcatcgg acttttataa tccataaaaa tatagctcgg gcgttgtccc gagctttttt
    12721 tatatagtat actgttcata aacaaatatc tggagggaga aaatggttca atcaatcaat
    12781 acaaaagtag acttggtaat tgatgctact tcgtttacgg gtctgacaga ttacgggcaa
    12841 atcatgatag gtaataatgg ttttgagttt taccatgcgc gagatagacg caaatatatc
    12901 caaattccat gggaagaagt ggattatgta ctagcatcag tcatgctaaa gggaaaatgg
    12961 attcctcgat atgccattca aacgaaaaaa aatggtacat acacattttc ttccaaagaa
    13021 cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg aagatagaat ggtacaatca
    13081 ttgagtttct ttgatgtagt gaaacgtggt attcgtagta tgtttaaacg caagtaaaaa
    13141 ataaagaaaa cttctaagag gaggttttct ttattttttt actttgcaga agaaaatatg
    13201 aaatagcaaa accaattatt tgtccccttt aaatcccata tgctaaaatt gattagcaaa
    13261 gaaggaggtt atattatgag ttggttatgg acattgattg ttggagccgt gatcggagca
    13321 attgcaggtg cgatcaccag taaaggaaat tcgatgggct gtgtaacgaa tatcattgct
    13381 ggactagtcg gttcaagtat tggacaagcg attttcggcg aatggggacc aaaagctgca
    13441 ggtatggcac ttattccatc tattttaggc gcagtgattt tggtagcagt tgtctcattt
    13501 ttctttggaa aacgatcata agtttttaca aaatagtaaa ctaaaaggag ttgcagcgag
    13561 actgtgactc ctttttggct ttatttattt aaaataaaaa gaagttttcc ggaaattttt
    13621 atttaactgt cgtgtacgaa gaaaagtttg ctataatgga aaaaagcgtg caaagaaaga
    13681 gggagtttca ttatgatgct acgtgtagaa aaattgcgca aaaaaatgca agaagaaaat
    13741 ttggattcat ttttggttac cagtccgtac aatcttcgtt acttaacgaa ctttacaggt
    13801 acgacaggat tagctgttat tactttggaa aaagcatttt ttattactga ttttcgttat
    13861 accgagcagg cggcagcgca ggctcaaggg tttgaaatta ttaaaaatgt cggacctatt
    13921 tttgatgagg tagcaaactt agtacgcaaa gaagaactgc actcattagc ttttgaggaa
    13981 acaacagtct catttttaga atattcggtt ttagaagaaa tcatcgatgc ggaattagta
    14041 cctgtttcac agatgatcga agaactcaga gaagtgaaag atgaagaaga aatcgctatt
    14101 atcgaaaaag catgtcatat tgcggatatg gcatatgatc acatcctgaa aatgatccaa
    14161 ccgggcatga ctgagattga agtcgcaaat caactagact tttatatgcg ttctcttggc
    14221 gcttcaggtg tttcttttga tacgatcgtt gctagcggat tgcgttcggc tatgccgcat
    14281 ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga tcacattgga ctttggttgc
    14341 tattacgaag ggtacgtttc cgatatgaca cgtacattcg ctattggtga tcctggggaa
    14401 aaattaaaag aaatttatca aatagtctta gatgctcaat tagcagttat tgatgctgca
    14461 aaaccaggaa tgactggtgt ccaattagat gcagttgcta gagattatat ttcaaaacat
    14521 ggttacgggg aagcatttgg acattctaca ggacatggga tcggtttaga gatccacgaa
    14581 ggaccaaacg tttcttctcg tgcagaaaag caatttgtac caggaaatgt gatcactgat
    14641 gaaccaggga tctacttacc aggaatcggt ggagtacgta ttgaggatga cttgttgatc
    14701 acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag aattgatcat cttatagttt
    14761 catcaataga agaagtaagc gtaaaaatca gtgttcacag actttgttat agaaaatcac
    14821 tcgatttaat gatacactat agaagaactt ttaagctgta agagataata aagttgggtg
    14881 ttttctccaa ttcatagcag tttgcagctg atcggcggca gttttcttac cacgtgatca
    14941 ctatgccgca tcatagagaa ttcggatgta tctcgtgctt aggcaaaggg aggaataaat
    15001 aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca agatttagga gagatcgtga
    15061 tcgctcctga agtcatcgaa gtaattatcg ggattgcagc ttctaaagta gaaggcgtat
    15121 acggaatgcg cggaactttt gccagcaatg tcactgaatt attaggacgt gcagctcacg
    15181 gaaaaggtgt ttacttagca aacgaggaag atggcttgaa agtagatctt tactgttatc
    15241 tagaatatgg tgtttcagtt cctaaagtag caatggaaat gcaggaacgg gtcagccagc
    15301 aagtcctttt catgacggat atcgatttat ctgaagtcaa tatccatgta gtagctgttg
    15361 ttcctgaaaa attagaacaa ccaacaatcg aagaactttt tgaagacgaa gaggaagaga
    15421 atgaataaag aattatcaag acacgagatt cgggaaatgg cattgcaggc tttatttcca
    15481 ttagacttca atgcagacct aacaaaagaa gatgcgattt tcaacgcaat cgaattagac
    15541 caccgcgaca tgatcaacga agatgaatca gagtttgttc ctgtttatct tgatacgcta
    15601 gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag tgattgaaaa acatttaaaa
    15661 aacaattggc acatcaatcg gatctcaaaa atggatttgg tgatcttgcg tatcgcgatt
    15721 tttgaaatga cttatgtgac agatgtacca gcggctgttg ctttaaatga agcaatcgag
    15781 ttggcaaaaa catttagtga tgaccgttca cgtaaattcg tcaacggtgt tttatccaat
    15841 gtattgaaag aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa
    15901 acctgttata aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt
    15961 ggttagatgg cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt
    16021 gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg
    16081 gtaggggaaa atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa
    16141 atcggaatcc attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt
    16201 gcagaaattg aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca
    16261 ttgccaaagc atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta
    16321 gacggttttc atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct
    16381 tgtactccat acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa
    16441 cgagcagtag ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg
    16501 atggcagatg caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca
    16561 caagaagcag atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc
    16621 gtaaaaccag gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg
    16681 atcggtgatg tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca
    16741 aagggtgtag ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa
    16801 gcttttggag caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag
    16861 tatttgaaaa gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa
    16921 atatcgaatt tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat
    16981 gcaaaaatat ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa
    17041 gaaggaatga aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat
    17101 caaatctatg tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa
    17161 gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca
    17221 cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc
    17281 atcacgacgg tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta
    17341 caaggaaacc aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc
    17401 aatttcgata caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt
    17461 aatgaagagc gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta
    17521 ggacatgaaa cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca
    17581 acagcagctg cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa
    17641 cggcaagcta gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc
    17701 gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc
    17761 atcaaattag accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg
    17821 aaagaaaaac aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga
    17881 gttaaagcag ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa
    17941 caatatatga aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg
    18001 agtccattga aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta
    18061 aaaagtgtca ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc
    18121 gttaaaggac aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt
    18181 tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc
    18241 attagaatca gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga
    18301 tacattagct aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt
    18361 tgattttgac ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc
    18421 tacctgctgt agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac
    18481 tctatgatgc gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac
    18541 ttttaacggc cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag
    18601 ctgctttaga aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca
    18661 atgatgatct tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta
    18721 tattggccgg agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag
    18781 gtaattctcc taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta
    18841 tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat
    18901 tagcatttat tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg
    18961 gcatattagc gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt
    19021 taggtttagc ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat
    19081 tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag
    19141 gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag
    19201 acaaattaga agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc
    19261 aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt
    19321 caaacaagga ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat
    19381 ttacaacgaa aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga
    19441 attaagagtc aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa
    19501 agcactcaaa gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc
    19561 cactggcgga tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga
    19621 cgttggctac aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga
    19681 aagagtgaat tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc
    19741 tgttatcgat gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt
    19801 aaaagaagag ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc
    19861 tgtagggaaa aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat
    19921 tttgtcattt gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac
    19981 ggggggagaa ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac
    20041 aatcaattca gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca
    20101 taaataaatt gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc
    20161 aaggaaataa gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa
    20221 aaaaagatcg gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg
    20281 aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca
    20341 gagatataaa ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata
    20401 gcataccagc tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg
    20461 ctttcgtggc agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg
    20521 cttcggcagc agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca
    20581 atgatgacga taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga
    20641 aagacttcct gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat
    20701 tactaacttt gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct
    20761 gaccggagag actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg
    20821 aggaagaggt tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt
    20881 gtttgaacta ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac
    20941 agatgaggac aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg
    21001 agtgaatggt catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga
    21061 tatccaagga caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt
    21121 ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat
    21181 ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt
    21241 tcagagaata gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt
    21301 cggagaagaa gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc
    21361 tgatggatta gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg
    21421 tgttggcttg gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc
    21481 aatttatgaa aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag
    21541 tcgtcagatt gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt
    21601 agaattaatc cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta
    21661 ctatgacgaa atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa
    21721 aatgaaagag gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca
    21781 tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag
    21841 tctgtatatg gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt
    21901 gaatcatgat ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact
    21961 gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa
    22021 aactattttt tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg
    22081 tgtgagcgga agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc
    22141 tcaagttttg tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta
    22201 tattgtgaaa gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa
    22261 agaaagagaa gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact
    22321 cgaacatgca aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt
    22381 caggtccttt tcagcagctg atagctatat tttattttta catgacactc attacagcag
    22441 tcaatacaac agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt
    22501 tagcaaaagt acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt
    22561 tgattcaagt gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc
    22621 ctttttttaa ataataggta gttacgatac aaataaataa tgttaataga actagccata
    22681 taaaggcatc atccctttca gcaataggta acttaatgtt cattccatag atactgccga
    22741 cgattgtcgg tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca
    22801 gattattcga tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa
    22861 tgcgagaagt agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca
    22921 aagcatgatt ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag
    22981 cagaagtcgc ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt
    23041 gtttgttttc agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg
    23101 tagctaaata actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga
    23161 gattaggcga aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg
    23221 gttcatgatt cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt
    23281 atgtatccac ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata
    23341 ataacgctgg ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat
    23401 cgtccaatat ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa
    23461 tctcttcttg attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg
    23521 tattacgcag tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc
    23581 ctgtttcttt tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat
    23641 agaatataac tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata
    23701 cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg
    23761 ttttggtata tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt
    23821 tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc
    23881 ggtatgcatg cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt
    23941 tctgttttgc taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt
    24001 atttatccgc gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg
    24061 tacgctgtct tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt
    24121 aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa
    24181 aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa
    24241 ttttagttag tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat
    24301 tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt
    24361 tgttctgaca cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa
    24421 tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg
    24481 attcttctat tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc
    24541 cagcacgtta agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa
    24601 ccgaattgaa atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa
    24661 ctttgacgaa attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga
    24721 ctaatgactg aaacatttca acactataca gtcatgttga aagaaacggt agatggattg
    24781 aacatcaaac cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa
    24841 tatttattaa gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa
    24901 gcaatcgatc acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt
    24961 atcaaagcga acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac
    25021 ggcattttgt atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt
    25081 agttatcatc aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct
    25141 tatcacgtaa ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc
    25201 gaggagaaat tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca
    25261 atccaaacaa ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga
    25321 agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac
    25381 gaactcggtg ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga
    25441 agaatcagcg taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa
    25501 gaatatagta cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa
    25561 ccagaactga aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa
    25621 aacaatcgat cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag
    25681 tagaatgatg gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt
    25741 ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca
    25801 atcaccggca aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt
    25861 ggcgattgct attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg
    25921 agctgaaaaa gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac
    25981 gagtttagaa caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga
    26041 agaaaaagga ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa
    26101 aaataaaatt cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa
    26161 agtaggtatc attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt
    26221 ctcgtatatc gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca
    26281 gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg
    26341 tgtggcgctc gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata
    26401 tacctctggg gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat
    26461 cctgcataag aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc
    26521 aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac
    26581 caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga
    26641 ccatcaggct cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt
    26701 tccagacaaa gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga
    26761 tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct
    26821 gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt
    26881 agacagcaga ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca
    26941 gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg
    27001 acaacgacct actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg
    27061 gcgtaatttc ttggtacaag atagttatga accaggatca accatgaaag ttttcactac
    27121 tgcagctgct atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat
    27181 ccaagtagaa gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat
    27241 gcgtcaagct ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg
    27301 cggacgatgg tataactatt tacaaaaact gggatttgga caaagtactc attctggatt
    27361 agatgatgaa gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc
    27421 ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc
    27481 gaataacggt acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg
    27541 agaagagcga acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga
    27601 aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg
    27661 tgtatatagt gtacctggat acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc
    27721 tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt
    27781 tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga
    27841 tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga
    27901 aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg
    27961 taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt
    28021 gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc
    28081 tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg
    28141 gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg
    28201 agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact
    28261 tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac
    28321 aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta
    28381 ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc
    28441 acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt
    28501 tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc
    28561 tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca
    28621 agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa
    28681 tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac
    28741 tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt
    28801 caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct
    28861 cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt
    28921 gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga
    28981 tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa
    29041 tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa
    29101 gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc
    29161 caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt
    29221 ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg
    29281 gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa
    29341 agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat
    29401 ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta
    29461 ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat
    29521 ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta
    29581 attacggaag ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga
    29641 acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat
    29701 caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa
    29761 gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca
    29821 gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat
    29881 ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa
    29941 gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa
    30001 gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat
    30061 tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt
    30121 aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca
    30181 aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat
    30241 gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg
    30301 actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta
    30361 gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc
    30421 gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca
    30481 atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat
    30541 ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa
    30601 actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga
    30661 gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc
    30721 tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg
    30781 agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt
    30841 ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt
    30901 tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat
    30961 cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc
    31021 aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata
    31081 tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc
    31141 agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt
    31201 gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg
    31261 agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta
    31321 ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc
    31381 tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc
    31441 cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg
    31501 gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc
    31561 aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga
    31621 atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg
    31681 ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa
    31741 acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa
    31801 ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa
    31861 tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat
    31921 acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc
    31981 cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca
    32041 tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta
    32101 tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat
    32161 tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac
    32221 agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat
    32281 tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca
    32341 aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt
    32401 gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat
    32461 gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca
    32521 aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt
    32581 tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat
    32641 gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg
    32701 caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg
    32761 catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa
    32821 agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca
    32881 caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca
    32941 agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac
    33001 aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc
    33061 agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat
    33121 ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg
    33181 atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata
    33241 ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg
    33301 ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc
    33361 agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg
    33421 gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg
    33481 cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac
    33541 aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa
    33601 gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta
    33661 aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga
    33721 ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg
    33781 gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc
    33841 aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca
    33901 atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg
    33961 aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact
    34021 tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg
    34081 accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc
    34141 ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc
    34201 caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt
    34261 cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag
    34321 cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac
    34381 ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata
    34441 aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga ataagatatg
    34501 gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt
    34561 ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc
    34621 attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa
    34681 ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa
    34741 gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc
    34801 atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc
    34861 gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga
    34921 ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca
    34981 ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt
    35041 gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg
    35101 atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac
    35161 caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca
    35221 acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta
    35281 cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat
    35341 atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa
    35401 gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag
    35461 gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact
    35521 gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat
    35581 tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat
    35641 atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat
    35701 gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat
    35761 tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg
    35821 tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga
    35881 agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga
    35941 aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca
    36001 gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt
    36061 aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt
    36121 aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt
    36181 ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa
    36241 agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct
    36301 tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca
    36361 agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt
    36421 caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt
    36481 ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa
    36541 aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg
    36601 ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta
    36661 ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag
    36721 cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa
    36781 tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg
    36841 ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg
    36901 ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg
    36961 agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg
    37021 cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt
    37081 acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt
    37141 gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg
    37201 cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac
    37261 aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat
    37321 atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag
    37381 ataggagggg aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac
    37441 atttatcgat tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct
    37501 gaccgaattt cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag
    37561 tgatttgcgt ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat
    37621 tttccccgat tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca
    37681 ttatcctagc aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac
    37741 ggggatcaaa cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt
    37801 tattgcaaat gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc
    37861 tgtacgcttg gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca
    37921 agaaagagga acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat
    37981 ttccagacag cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga
    38041 aaatacaaga cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg
    38101 cagagtccag atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt
    38161 cggtgtatta cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat
    38221 atccaaaata agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat
    38281 ttcttagatt tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa
    38341 aaagcagtca aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg
    38401 aaccaatcaa tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag
    38461 gaatccgaag tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct
    38521 gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca
    38581 gatgcaacag aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca
    38641 cgagtattcc atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt
    38701 ccggaatggg atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg
    38761 atcaaagaag ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac
    38821 acaggagtca tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc
    38881 gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag
    38941 ccgatgagag tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct
    39001 gcttttccga aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt
    39061 gcactagata gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg
    39121 aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa
    39181 gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa
    39241 ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac
    39301 gaaggaaaac ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat
    39361 ttaggacatt cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct
    39421 ggctatcgtg caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta
    39481 ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt
    39541 cgtgaatatg cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt
    39601 gcaggagatt gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta
    39661 cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac
    39721 tggtctccgt caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa
    39781 tctccatcta tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat
    39841 acagcttttg tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct
    39901 gtaaatccaa aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg
    39961 aaagatttgc tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa
    40021 gaatttgctg gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa
    40081 tcacttgtga ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact
    40141 gctccaggac atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg
    40201 tcacctgtag actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt
    40261 tatgacaaag caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag
    40321 ctagatttct ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat
    40381 cgggcaactc cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa
    40441 gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt
    40501 gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc
    40561 tatgctgaaa atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta
    40621 tttgctgaac atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa
    40681 ggatttaccc acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat
    40741 gtctggtttg attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca
    40801 ttcccagcag atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt
    40861 atcacgacta gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga
    40921 tttgctttag atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca
    40981 gaaaaagtga tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat
    41041 tatgaagcag atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt
    41101 aaaatcagaa atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa
    41161 gatactgttt cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac
    41221 caagtaatca aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac
    41281 cgtacagtta tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa
    41341 gatgtagttt atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc
    41401 tatcaaacag ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa
    41461 gaaatctggt cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt
    41521 tatgaagagt ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc
    41581 cgtgataatg tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct
    41641 ttggaagcaa aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg
    41701 gatgcagata ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca
    41761 gatgaacaag tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa
    41821 gcagacgggg aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa
    41881 aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct
    41941 gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca
    42001 cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg
    42061 ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga
    42121 tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc
    42181 cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt
    42241 caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact
    42301 atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt
    42361 caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag
    42421 tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt
    42481 gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa
    42541 aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt
    42601 ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa
    42661 ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat
    42721 gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac
    42781 tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt
    42841 aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa
    42901 agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc
    42961 aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa
    43021 aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta
    43081 atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt
    43141 ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata
    43201 gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt
    43261 ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt
    43321 agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta
    43381 gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt
    43441 tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt
    43501 accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat
    43561 attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca
    43621 ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg
    43681 atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg
    43741 atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca
    43801 acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag
    43861 ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca
    43921 tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt
    43981 ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa
    44041 ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca
    44101 gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct
    44161 agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc
    44221 atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta
    44281 aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag
    44341 actcattctt tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat
    44401 aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag
    44461 ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca
    44521 gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt
    44581 tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac
    44641 ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa
    44701 ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca
    44761 tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga
    44821 ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga
    44881 aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa
    44941 tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa
    45001 tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc
    45061 tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt
    45121 taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat
    45181 ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca
    45241 ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa
    45301 aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg
    45361 attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt
    45421 gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc
    45481 actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg
    45541 tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc
    45601 ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg
    45661 aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt
    45721 gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg
    45781 ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat
    45841 ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg
    45901 aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat
    45961 ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc
    46021 aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt
    46081 cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat
    46141 cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc
    46201 agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac
    46261 tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca
    46321 gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct
    46381 agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga
    46441 aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt
    46501 tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat
    46561 cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa
    46621 attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa
    46681 aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca
    46741 gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt
    46801 aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg
    46861 ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa
    46921 tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt
    46981 cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg
    47041 gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa
    47101 gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct
    47161 ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt
    47221 gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga
    47281 aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga
    47341 cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta
    47401 ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat
    47461 cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca
    47521 cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg
    47581 aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga
    47641 atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa
    47701 cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg
    47761 caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa
    47821 actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt
    47881 aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat
    47941 gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac
    48001 gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg
    48061 tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca
    48121 aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa
    48181 agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg
    48241 ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc
    48301 caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg
    48361 ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg
    48421 tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg
    48481 ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag
    48541 cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa
    48601 tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac
    48661 atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta
    48721 cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg
    48781 atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac
    48841 gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata
    48901 tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta
    48961 ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag
    49021 gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc
    49081 agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa
    49141 acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg
    49201 agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg
    49261 tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt
    49321 taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag
    49381 ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata
    49441 tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag
    49501 caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa
    49561 tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa
    49621 acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga
    49681 tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg
    49741 aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga
    49801 cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt
    49861 atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag
    49921 aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc
    49981 tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc
    50041 gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc
    50101 agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag
    50161 aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa
    50221 ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt
    50281 ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta
    50341 aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc
    50401 gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg
    50461 gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag
    50521 aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg
    50581 atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag
    50641 atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact
    50701 aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca
    50761 tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt
    50821 aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca
    50881 gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg
    50941 gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat
    51001 gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa
    51061 cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac
    51121 ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg
    51181 ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg
    51241 gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg
    51301 agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac
    51361 aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg
    51421 ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca
    51481 tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggaagaag
    51541 aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta tataaaaaac
    51601 ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat acatcctgcc
    51661 ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg
    51721 catactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga
    51781 tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca aaggaatctt
    51841 tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta cactagcaat
    51901 cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt atggagtaaa
    51961 tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt cctttggtcc
    52021 agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg caatcaatag
    52081 ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat ggggttggct
    52141 aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg gagctatttc
    52201 agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc ctgattcagt
    52261 accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg ctttatacgt
    52321 cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga tcattgattt
    52381 ggtgcaaaaa tatattgcag aaccattcct aggattatct caagggttgg gagcagtatt
    52441 gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggat caaatgtatt
    52501 ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg atattttcca
    52561 aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg aagatggaaa
    52621 agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg gatcaggcgg
    52681 aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt atctaacagt
    52741 agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga tgtttggtct
    52801 gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag tagtagcaac
    52861 agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc agcaagtcac
    52921 atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt ggcgggcacc
    52981 gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc
    53041 tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg aggagcggga
    53101 cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg attacaattg
    53161 ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat gaagaaggaa
    53221 tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag
    53281 atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct tggggaacag
    53341 tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg atggttgaga
    53401 tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg tatagtttga
    53461 atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt ctaacaggat
    53521 taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag aaaaataact
    53581 aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata aagttatgga
    53641 ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa aacagctgaa
    53701 aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa aaatcgaaga
    53761 agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg aattcgtcgt
    53821 tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca cagaaaaaat
    53881 ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta
    53941 ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt attaaatcaa
    54001 tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa tttttcatag
    54061 gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg cagcaaaatg
    54121 acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga tttagccact
    54181 tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac acttgcggag
    54241 aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca agtcatggcg
    54301 accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat tggctatgtt
    54361 gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga aaagatcctt
    54421 gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc ctatcccttc
    54481 tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact aaacagtcgt
    54541 gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt tgaagatgca
    54601 aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc tggtctgaag
    54661 cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca gtttgctttg
    54721 cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc aacgtcttcc
    54781 tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct tgttcgcttc
    54841 gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta cggcggtaat
    54901 gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc ttatatgatg
    54961 gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa gagacaagct
    55021 gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa
    55081 gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa actcccaatc
    55141 gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa agtggaagag
    55201 ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt aagctcaatc
    55261 ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg tgatgacaat
    55321 atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt taaagtaaat
    55381 cctctttttt cgtaaatttg aattttttgt tttcttttgt tttagggttt tcttttttgt
    55441 ggtcttggat tgcttctttc gtaatctttt cccgattttt tttagatgaa tctaagcgtt
    55501 ctgcgtatac tttttctttt ggcttatcat tcattggaaa ggacctccct acattatttc
    55561 attctacctc tagtttatca tctgttaagg actagtggaa acgttttgcc taacttataa
    55621 gctgattttt ttagcagata agcttgcaag taaaaaacaa acaagctaca attgatttat
    55681 tgaaattgtt cctgtagctc agtaggatag agcaatcgcc tcctaagcga taggtcgctg
    55741 gttcaattcc agtcaggaac gtataaataa cccctgtatc ctttgcggta caggggtttg
    55801 ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat ttttatataa actatcgaac
    55861 acagtattga ttttttcttt atcctgatct tcaagctctc gaacaatatg aagataagta
    55921 gacattgtag tttctaaa
//
LOCUS       DASJST010000002        55502 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJST010000002 DASJST010000000
VERSION     DASJST010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.002"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            558..3197
                     /locus_tag="U4B05_000062"
     CDS             558..3197
                     /locus_tag="U4B05_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2049231.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="U4B05_000063"
     CDS             complement(3365..4063)
                     /locus_tag="U4B05_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2049232.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="U4B05_000064"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="U4B05_000064"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN2049233.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="U4B05_000065"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="U4B05_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN2049234.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="U4B05_000066"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="U4B05_000066"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN2049235.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="U4B05_000067"
     CDS             9034..10140
                     /locus_tag="U4B05_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN2049236.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="U4B05_000068"
     CDS             10157..11185
                     /locus_tag="U4B05_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN2049237.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="U4B05_000069"
     CDS             11191..11670
                     /locus_tag="U4B05_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN2049238.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="U4B05_000070"
     CDS             11686..12981
                     /locus_tag="U4B05_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN2049239.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="U4B05_000071"
     CDS             12992..13816
                     /locus_tag="U4B05_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2049240.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="U4B05_000072"
     CDS             13981..14880
                     /locus_tag="U4B05_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2049241.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="U4B05_000073"
     CDS             complement(15006..15875)
                     /locus_tag="U4B05_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN2049242.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="U4B05_000074"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="U4B05_000074"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN2049243.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="U4B05_000075"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="U4B05_000075"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN2049244.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="U4B05_000076"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="U4B05_000076"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN2049245.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="U4B05_000077"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="U4B05_000077"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN2049246.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="U4B05_000078"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="U4B05_000078"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN2049247.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="U4B05_000079"
     CDS             21273..22031
                     /locus_tag="U4B05_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN2049248.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="U4B05_000080"
     CDS             22131..22775
                     /locus_tag="U4B05_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN2049249.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="U4B05_000081"
     CDS             22782..23789
                     /locus_tag="U4B05_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN2049250.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="U4B05_000082"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="U4B05_000082"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN2049251.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="U4B05_000083"
     CDS             24667..25470
                     /locus_tag="U4B05_000083"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN2049252.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /locus_tag="U4B05_000084"
     CDS             complement(25719..26783)
                     /locus_tag="U4B05_000084"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN2049253.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="U4B05_000085"
     CDS             complement(26780..27865)
                     /locus_tag="U4B05_000085"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN2049254.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="U4B05_000086"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="U4B05_000086"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN2049255.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="U4B05_000087"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="U4B05_000087"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN2049256.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30108..30767
                     /locus_tag="U4B05_000088"
     CDS             30108..30767
                     /locus_tag="U4B05_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2049257.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            30852..31757
                     /locus_tag="U4B05_000089"
     CDS             30852..31757
                     /locus_tag="U4B05_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2049258.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="U4B05_000090"
     CDS             complement(31759..32391)
                     /locus_tag="U4B05_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2049259.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    32542..32667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="U4B05_000091"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="U4B05_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN2049260.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="U4B05_000092"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="U4B05_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN2049261.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="U4B05_000093"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="U4B05_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN2049262.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="U4B05_000094"
     CDS             34171..35508
                     /locus_tag="U4B05_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN2049263.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="U4B05_000095"
     CDS             35556..37685
                     /locus_tag="U4B05_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN2049264.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="U4B05_000096"
     CDS             37660..38349
                     /locus_tag="U4B05_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049265.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="U4B05_000097"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="U4B05_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="U4B05_000098"
     CDS             complement(39506..39700)
                     /locus_tag="U4B05_000098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049266.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="U4B05_000099"
     CDS             complement(39750..40190)
                     /locus_tag="U4B05_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN2049267.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="U4B05_000100"
     CDS             complement(40194..41039)
                     /locus_tag="U4B05_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN2049268.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="U4B05_000101"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="U4B05_000101"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN2049269.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="U4B05_000102"
     CDS             complement(42663..43610)
                     /locus_tag="U4B05_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2049270.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43833..44183)
                     /locus_tag="U4B05_000103"
     CDS             complement(43833..44183)
                     /locus_tag="U4B05_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2049271.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44383..45462
                     /gene="pepA"
                     /locus_tag="U4B05_000104"
     CDS             44383..45462
                     /gene="pepA"
                     /locus_tag="U4B05_000104"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN2049272.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45606..45926
                     /locus_tag="U4B05_000105"
     CDS             45606..45926
                     /locus_tag="U4B05_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2049273.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45948..46412
                     /locus_tag="U4B05_000106"
     CDS             45948..46412
                     /locus_tag="U4B05_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2049274.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46617..47222
                     /locus_tag="U4B05_000107"
     CDS             46617..47222
                     /locus_tag="U4B05_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN2049275.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47261..48550)
                     /locus_tag="U4B05_000108"
     CDS             complement(47261..48550)
                     /locus_tag="U4B05_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN2049276.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48978..49457
                     /gene="rlmH"
                     /locus_tag="U4B05_000109"
     CDS             48978..49457
                     /gene="rlmH"
                     /locus_tag="U4B05_000109"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN2049277.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            49542..50051
                     /locus_tag="U4B05_000110"
     CDS             49542..50051
                     /locus_tag="U4B05_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEN2049278.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            50151..50801
                     /locus_tag="U4B05_000111"
     CDS             50151..50801
                     /locus_tag="U4B05_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049279.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            51252..52190
                     /locus_tag="U4B05_000112"
     CDS             51252..52190
                     /locus_tag="U4B05_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2049280.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            52203..53255
                     /locus_tag="U4B05_000113"
     CDS             52203..53255
                     /locus_tag="U4B05_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2049281.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            53521..54156
                     /locus_tag="U4B05_000114"
     CDS             53521..54156
                     /locus_tag="U4B05_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN2049282.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            54347..55138
                     /gene="msrA"
                     /locus_tag="U4B05_000115"
     CDS             54347..55138
                     /gene="msrA"
                     /locus_tag="U4B05_000115"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2049283.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        17619 a         9922 c        11894 g        16067 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    43801 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    43861 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    43921 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    43981 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    44041 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    44101 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    44161 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    44221 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    44281 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    44341 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    44401 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    44461 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    44521 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    44581 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    44641 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    44701 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    44761 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    44821 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    44881 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    44941 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    45001 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    45061 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    45121 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    45181 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    45241 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    45301 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    45361 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    45421 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    45481 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    45541 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    45601 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    45661 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    45721 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    45781 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    45841 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    45901 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    45961 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    46021 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    46081 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    46141 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    46201 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    46261 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    46321 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    46381 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    46441 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    46501 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    46561 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    46621 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    46681 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    46741 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    46801 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    46861 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    46921 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    46981 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    47041 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    47101 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    47161 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    47221 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    47281 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    47341 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    47401 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    47461 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    47521 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    47581 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    47641 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    47701 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    47761 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    47821 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    47881 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    47941 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    48001 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    48061 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    48121 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    48181 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    48241 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    48301 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    48361 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    48421 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    48481 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    48541 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    48601 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    48661 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    48721 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    48781 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    48841 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    48901 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    48961 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    49021 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    49081 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    49141 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    49201 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    49261 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    49321 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    49381 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    49441 cgccttatca taagtgatgc ggtttttatc attgatccta aaaatcttag caagacaaaa
    49501 agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac aatgataggt
    49561 tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa gcttttgagc
    49621 taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa gtacaggata
    49681 agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac caagtttttg
    49741 acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat aagccgatag
    49801 tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat
    49861 taaaaatcgc cttgtctatg accactgatg atattttaga cgtttttgct gaggcagaaa
    49921 tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa cgaaatttta
    49981 agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt tataatcgga
    50041 gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat gagagctagt
    50101 ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt atgaatgatg
    50161 aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga tgtaggctca
    50221 attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta gcgaataaat
    50281 cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt agtagaagcg
    50341 aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg ttaaataaga
    50401 gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata attgattcat
    50461 taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat ggaatggtca
    50521 ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt ttttatcaac
    50581 tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa tatggcaatg
    50641 aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct tggataattc
    50701 cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa ctaattaata
    50761 aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt tttcatgata
    50821 atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt aaataagtgt
    50881 attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc aacatcgtac
    50941 tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga aggttttagt
    51001 atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt tctatgaatg
    51061 tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat tctattccag
    51121 tttttattga tttgctgata aaataacttg aatggtggat ataaacatga taaataagag
    51181 aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga aagaaaaagg
    51241 aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt cgctttgggt
    51301 taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggca gaacatgtcc
    51361 aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta gaagcaaaac
    51421 taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg attattctat
    51481 ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta atcaaaaaag
    51541 atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt gagaaatttg
    51601 taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt gaaggtcctg
    51661 gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt gatggaaaag
    51721 aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat gctcattttt
    51781 ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca atgaacggac
    51841 tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact gctcataaaa
    51901 tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag attgaactag
    51961 atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca attgggttac
    52021 attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa atcgattata
    52081 tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct tattgtgatt
    52141 tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa aggagagatt
    52201 gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc ctcgctggta
    52261 cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc attttaaaat
    52321 attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt tttcaactag
    52381 caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat ccgatgagaa
    52441 tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc acacctgatt
    52501 tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat acgatgctga
    52561 cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg tcagtgtcga
    52621 ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt tatttattcc
    52681 cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc actaccttac
    52741 aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt atctctccta
    52801 tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct ggtgcggctg
    52861 cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag gttaataagt
    52921 caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct aatctattta
    52981 gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct attcctgtcg
    53041 ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc ggacttgttg
    53101 gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt tgttggttag
    53161 tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta tttaaactgt
    53221 atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga aattttcaat
    53281 caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg cctttttgag
    53341 taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta aaatgtagaa
    53401 gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa tatgtgttaa
    53461 aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta ggagaagaaa
    53521 atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat tagtgattta
    53581 tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac taccgtaatt
    53641 agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg gtcagttgta
    53701 tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat tggaacatca
    53761 aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg tgattgtatt
    53821 tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc gacttggatc
    53881 cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt gttacttgtt
    53941 ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt tttaacagtt
    54001 attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat tagtcagttt
    54061 gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa ctatttgccg
    54121 atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga agctgattaa
    54181 gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa gaatagagat
    54241 gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa tttcaggtag
    54301 tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt tagatgtgga
    54361 gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag aatgggaaag
    54421 aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc aggtgataga
    54481 aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac cagatgcgat
    54541 ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg attatgccaa
    54601 acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg gctgtttctg
    54661 gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat ctggttatac
    54721 aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata ctgggcatgt
    54781 tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc tgttggagat
    54841 ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc gggggaaaca
    54901 atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg aaaaatcaag
    54961 agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga ttcgccaagc
    55021 aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc aacctaaaag
    55081 atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac atagatagag
    55141 gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga caaaaatagc
    55201 ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga acaccgagga
    55261 aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg tatgacaagt
    55321 cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag agaattcgaa
    55381 ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata attaatagac
    55441 acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc aaacaataac
    55501 ta
//
LOCUS       DASJST010000003        55439 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJST010000003 DASJST010000000
VERSION     DASJST010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.003"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..882
                     /locus_tag="U4B05_000116"
     CDS             7..882
                     /locus_tag="U4B05_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN2049284.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            962..1936
                     /locus_tag="U4B05_000117"
     CDS             962..1936
                     /locus_tag="U4B05_000117"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2049285.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2116..3261
                     /locus_tag="U4B05_000118"
     CDS             2116..3261
                     /locus_tag="U4B05_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2049286.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3398..3742
                     /locus_tag="U4B05_000119"
     CDS             3398..3742
                     /locus_tag="U4B05_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN2049287.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            4013..5299
                     /gene="efmA"
                     /locus_tag="U4B05_000120"
     CDS             4013..5299
                     /gene="efmA"
                     /locus_tag="U4B05_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN2049288.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5437..6036)
                     /locus_tag="U4B05_000121"
     CDS             complement(5437..6036)
                     /locus_tag="U4B05_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN2049289.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6440..7561
                     /gene="mnmA"
                     /locus_tag="U4B05_000122"
     CDS             6440..7561
                     /gene="mnmA"
                     /locus_tag="U4B05_000122"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN2049290.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7807..9228
                     /gene="arcD"
                     /locus_tag="U4B05_000123"
     CDS             7807..9228
                     /gene="arcD"
                     /locus_tag="U4B05_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN2049291.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9242..10360
                     /locus_tag="U4B05_000124"
     CDS             9242..10360
                     /locus_tag="U4B05_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN2049292.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10420..11712)
                     /locus_tag="U4B05_000125"
     CDS             complement(10420..11712)
                     /locus_tag="U4B05_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2049293.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            11844..12422
                     /locus_tag="U4B05_000126"
     CDS             11844..12422
                     /locus_tag="U4B05_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2049294.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            12551..12928
                     /locus_tag="U4B05_000127"
     CDS             12551..12928
                     /locus_tag="U4B05_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN2049295.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            12861..14933
                     /locus_tag="U4B05_000128"
     CDS             12861..14933
                     /locus_tag="U4B05_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2049296.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(15144..16091)
                     /locus_tag="U4B05_000129"
     CDS             complement(15144..16091)
                     /locus_tag="U4B05_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2049297.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            16337..16801
                     /locus_tag="U4B05_000130"
     CDS             16337..16801
                     /locus_tag="U4B05_000130"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN2049298.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(16863..17906)
                     /locus_tag="U4B05_000131"
     CDS             complement(16863..17906)
                     /locus_tag="U4B05_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN2049299.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            18257..19120
                     /locus_tag="U4B05_000132"
     CDS             18257..19120
                     /locus_tag="U4B05_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2049300.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(19249..20871)
                     /locus_tag="U4B05_000133"
     CDS             complement(19249..20871)
                     /locus_tag="U4B05_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2049301.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            21120..21641
                     /locus_tag="U4B05_000134"
     CDS             21120..21641
                     /locus_tag="U4B05_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2049302.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(21827..23557)
                     /gene="spxB"
                     /locus_tag="U4B05_000135"
     CDS             complement(21827..23557)
                     /gene="spxB"
                     /locus_tag="U4B05_000135"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN2049303.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            23722..24540
                     /locus_tag="U4B05_000136"
     CDS             23722..24540
                     /locus_tag="U4B05_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN2049304.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(24643..24840)
                     /locus_tag="U4B05_000137"
     CDS             complement(24643..24840)
                     /locus_tag="U4B05_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049305.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            25009..25587
                     /locus_tag="U4B05_000138"
     CDS             25009..25587
                     /locus_tag="U4B05_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2049306.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            25682..25819
                     /locus_tag="U4B05_000139"
     CDS             25682..25819
                     /locus_tag="U4B05_000139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049307.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(26632..27135)
                     /locus_tag="U4B05_000140"
     CDS             complement(26632..27135)
                     /locus_tag="U4B05_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2049308.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            27494..28519
                     /locus_tag="U4B05_000141"
     CDS             27494..28519
                     /locus_tag="U4B05_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2049309.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            28842..29720
                     /locus_tag="U4B05_000142"
     CDS             28842..29720
                     /locus_tag="U4B05_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN2049310.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            30196..30312
                     /locus_tag="U4B05_000143"
     CDS             30196..30312
                     /locus_tag="U4B05_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2049311.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            31240..31578
                     /locus_tag="U4B05_000144"
     CDS             31240..31578
                     /locus_tag="U4B05_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049312.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            31672..31902
                     /locus_tag="U4B05_000145"
     CDS             31672..31902
                     /locus_tag="U4B05_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049313.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            32302..32673
                     /locus_tag="U4B05_000146"
     CDS             32302..32673
                     /locus_tag="U4B05_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN2049314.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(32947..33639)
                     /locus_tag="U4B05_000147"
     CDS             complement(32947..33639)
                     /locus_tag="U4B05_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2049315.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(33642..34427)
                     /locus_tag="U4B05_000148"
     CDS             complement(33642..34427)
                     /locus_tag="U4B05_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2049316.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            34664..36064
                     /gene="lacG"
                     /locus_tag="U4B05_000149"
     CDS             34664..36064
                     /gene="lacG"
                     /locus_tag="U4B05_000149"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN2049317.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            36089..36406
                     /locus_tag="U4B05_000150"
     CDS             36089..36406
                     /locus_tag="U4B05_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2049318.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            36419..38092
                     /locus_tag="U4B05_000151"
     CDS             36419..38092
                     /locus_tag="U4B05_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN2049319.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            38106..39737
                     /locus_tag="U4B05_000152"
     CDS             38106..39737
                     /locus_tag="U4B05_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN2049320.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(40228..41793)
                     /gene="guaA"
                     /locus_tag="U4B05_000153"
     CDS             complement(40228..41793)
                     /gene="guaA"
                     /locus_tag="U4B05_000153"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN2049321.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(41852..42775)
                     /locus_tag="U4B05_000154"
     CDS             complement(41852..42775)
                     /locus_tag="U4B05_000154"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN2049322.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            42971..43576
                     /locus_tag="U4B05_000155"
     CDS             42971..43576
                     /locus_tag="U4B05_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2049323.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            43884..44900
                     /locus_tag="U4B05_000156"
     CDS             43884..44900
                     /locus_tag="U4B05_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2049324.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            45041..46342
                     /locus_tag="U4B05_000157"
     CDS             45041..46342
                     /locus_tag="U4B05_000157"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN2049325.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            46388..47050
                     /gene="deoC"
                     /locus_tag="U4B05_000158"
     CDS             46388..47050
                     /gene="deoC"
                     /locus_tag="U4B05_000158"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN2049326.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            47071..47469
                     /locus_tag="U4B05_000159"
     CDS             47071..47469
                     /locus_tag="U4B05_000159"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN2049327.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            47535..48617
                     /locus_tag="U4B05_000160"
     CDS             47535..48617
                     /locus_tag="U4B05_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2049328.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            48829..50391
                     /locus_tag="U4B05_000161"
     CDS             48829..50391
                     /locus_tag="U4B05_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049329.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            50384..51517
                     /locus_tag="U4B05_000162"
     CDS             50384..51517
                     /locus_tag="U4B05_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049330.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            51517..52473
                     /locus_tag="U4B05_000163"
     CDS             51517..52473
                     /locus_tag="U4B05_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049331.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            52572..53738
                     /gene="deoB"
                     /locus_tag="U4B05_000164"
     CDS             52572..53738
                     /gene="deoB"
                     /locus_tag="U4B05_000164"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN2049332.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            53762..54580
                     /locus_tag="U4B05_000165"
     CDS             53762..54580
                     /locus_tag="U4B05_000165"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2049333.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            54597..55304
                     /gene="deoD"
                     /locus_tag="U4B05_000166"
     CDS             54597..55304
                     /gene="deoD"
                     /locus_tag="U4B05_000166"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2049334.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        17574 a         9802 c        11738 g        16325 t
ORIGIN      
        1 cttataatga aaaaaaggag gcgatcccaa ctgaatcaag tagtggataa acctttatat
       61 ttcaaagtag ataaaaaaat taaaaaatta gccagcactc agcagctgca gtcaagaaaa
      121 agtaaattgc tttttttagc tctggttttt gaagatcaaa gttatgtgat tatcgatcaa
      181 tcaggacatc cggtagaata ttctccagca aaatatactt atcaagaggg tctttcttgt
      241 aaaaaatgga agctgatcaa tgaagagcca atcgagttat ctagatggat caacagaaaa
      301 gaagatattc ccgttttgat cgaagaaaaa aggtcaggaa aggaactcgc aaattgctgg
      361 gtgggattgc cagaagaacg ttttttgagg tataagaaat gggctacccc ctcaggctat
      421 ctttgtggta cttacgcggc agcagttctc cttgcctatt atcaagacta tcgtaagggg
      481 tggatgcttc caaatgagat tcgcaagaaa aatacggctg attcgttggt tttgacgaag
      541 gcattgcgta gccagatcca accactagga cttcctacga ttccttttca ggttagtaca
      601 gggatttcta gttttttaaa gaagaatggc aatcatgagc gtgcacgagc aactcttcta
      661 ggttcatggc agcgagctac aaaacgtata cgtgaaggca aacctgtgat gattggtatt
      721 ttgaaagtac ttggcagtac atatggcaat cattgggtga cagcgtacgc ttattttgaa
      781 actgaaacag gagaacgtta ttacaaagta cacgacaact ggggcgatta tcataaagtc
      841 attcctgcca gttggagtaa cggaacagtt tcacttcctt aagaatttgc tgaaattaac
      901 agaaaatctt ggaaaaatat gttaaactat cgaagaatag agaagttttg gggaggacta
      961 catgagtaat aagtatcagg atgacacgtt gaagattttt agtttgaatg gcaaccgtcc
     1021 attagctgaa aaaatcgcga aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca
     1081 atttagtgat ggcgagattt ccatcaatat cgaagaaagt attcgagggg atcatgtgta
     1141 tatcgttcaa tcaacgaatg cacctgtcaa tgattactat atggaactac tgatcatgat
     1201 cgatgcaatg aagcgagcaa gtgcgaaaac gatcaatgtc gtattgcctt actatgggta
     1261 tgctaggcaa gaccgcacgg cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa
     1321 cttgatcgaa gaagcagggg caactcgagt actgacttta gatctgcaca cggttcaagt
     1381 acaaggattc tttgatattc ctgtagataa tttatttacg atgccgttgt ttgctcatta
     1441 ttaccgtcaa ttaggtctga cgggagatga catcgtagtt gtttcgccaa aaaacagcgg
     1501 tgttcagcgt gcacgtagtc tttcagaata tttgaacagc acattagcca tcgtagatca
     1561 tgcagatgat gcttcaactg agggtggtta tgtgatcggt gatgttcaag gaaagacatg
     1621 tatcatggta gatgatatct tgaacacagg agcaacgctt gcacgagcag caaatgtatt
     1681 aaaagaaaac ggcgcaaaag aagtatatgc ctgtgcgtca catggattat tgtccgatca
     1741 tgcgaaaagt attttagaca atgcaccaat caaagatatt tgtatcactg attctgtttt
     1801 cacagaagaa aatcgtcatc cagaaaactt aacaatcgtt acatgttcag aattaatggg
     1861 tgaagcggtc aaacgcatcc atgaaaatac accgatgagt ccgttattcc gtctggaaga
     1921 aaaagaattc gaataaacaa aaaaaagagg gaaagaagtt gtgacagaag tgagcagctt
     1981 caaggagctg cgacaagact tttgtcacag ctcctttttt ctatgcccat gaaacgtcct
     2041 ctagacaaaa atgagctctt ctttggtttg ttcgctattt tatgctagaa tgtgcctagc
     2101 aataatttgg aagggatgaa tgaagtgaaa actatttatc tggatcatgc ggcaactacc
     2161 cccatgcatc cacaagtaat tgaagcgatg acggcaatca tgcaagacac gtttggaaat
     2221 ccttcaagca tacatggatt tggtcgccac gcgcatgaaa aattggaaac agcaagacaa
     2281 atcattgcag acagtctgca agcaaaacct cacgagatca tctttaacag tgggggaaca
     2341 gaaggggata atacagctat cttagaaaca gcgttctcgc gtcaaaaaga aggcagacat
     2401 ctaatcacta cagctatcga acatcctgct gttcttaata ccatgaaata tttagagaca
     2461 aaggggtttg acgttacata tctgcctgta gatgaaaaag ggaatctgtc gatcgaagag
     2521 gtcaaaaaag cgttacgaga agatacgatc ttggtctctg tgatgtacgg gaacaatgaa
     2581 atcggaaatt tgatgccaat caaagaaatc ggtgaacttt tagcggatca tccagcttat
     2641 ttccatacag atgcggtaca agcgtacgga aaccaaacga tccatccaga agaattagga
     2701 atcgatttat taagtatctc tgctcataaa atcaatgggc caaaaggcgt cggtttctta
     2761 tataaacgag acggagtaaa tatccctgca ttgcttttag gcggggagca ggaagaaaag
     2821 cggcgtgcag gtacagaaaa tttagcaggg atctggggta tggcaaaagc agttgaactg
     2881 ctgtcacctg aagaacgtga agcacgtaac aacaaatata aaggattcca gcaattgatt
     2941 cttgatacat tagaagcaaa cgaaatccct tatcaagtaa atggtgattt aacgaataaa
     3001 ctccctcatg tgttgaatct tcgcttaatt ggcgaagcaa atgatttgct actgatgaag
     3061 cttgatctta atggggtcgc tatttctact ggttcagctt gtacagcagg aaatatcgag
     3121 ccttcacacg tattagaagc aatgtatgga gaagatacac cgatactaaa agaatccatc
     3181 cgtatcagct ttggtttggg aaataccgaa gacgacatcc agtatttcct tgatcaatta
     3241 gttcgtgctg tcaaaaaata actagtgaag tcattcttat atgaacagat gtctgagaga
     3301 acgccttcat tttttaggga acttaactga aaaacctagt aaaaatcagg ataaactttt
     3361 ataataaaga gaaactaatt ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac
     3421 ggtactgggc gcaccaatta gctatcgttt gcatcctgat gcaaaaagat atacattgcg
     3481 cgataatgga tttacagaaa caaacggagg caattatcaa ttgatccgac cattagatgc
     3541 cacgccgcaa agcaaagaag gattcaaatt aaaaatcact gtatcaaaag atattcaaac
     3601 attgaaaatg tcgatcacta cagcaaatgg attgaaagca gtcaatattt tcaaagatcc
     3661 aaaacagaaa atgagccaag ataaatttta ttttctgatg gatgggatga ttagccgtgg
     3721 gttattcgaa aaagtcgaat gatctgtgga tataaaaaga caactagttt tgaaacggcc
     3781 tattgttaga tgactaactt tgtaaggacg acgcaaatta gttgtctttt ttatttttaa
     3841 atttaacaca tgttgttatt tttaatattt tgctgattta ttgtttctaa gtaaagaaat
     3901 ttaaattgaa acgattgtta tttagggaaa aaggtatttt ttgaaaaaag aatcccgtat
     3961 aatgattgtt gtgcaaacgc tatacaaaat gaaaaagaaa agagggatgt ttatggaaaa
     4021 tgaacagtcg gtcgttttaa cgaattggaa gagaaattat ttgttctttt tatcgggaca
     4081 gtttttgtca ggaatcacta gtatggtcgt ccaatatgcg atcatctggt atttgacaag
     4141 agaaaccggt tcggcgacaa ttttgagttt tgctacttta ctaggaatga ttcccatggt
     4201 tttactaagc ccatttgtag gaccgctagt ggatcgctgg gataaaaaag ctcttttgat
     4261 tgtcacggat attattgtag cgattttcgc gcttatttta gcagtagtcg gaacgatttc
     4321 agaatccttc ccgatttggc tagtttttgt gtctttgttt atgcgttcgg tcgcacaaac
     4381 attccaaatg ccaacgatcc agtcgattat gccgacaatc gttccatctt ctcacataac
     4441 aaggacgaat ggacagttag gaatggtcca atcagcgaat ttcatcattg cgccagctct
     4501 aggtgcggct ttgttttcag tcgttcctgt caattacctt attttattag atgtattggg
     4561 ggcagttttt ggtgtcggct tattgatttt tgtgaagatc ccaaaagttt ctcctgaaat
     4621 attggaagtt ccgcttacta tttttaaaga tgcaaaattt gggctgcagc aattgatgga
     4681 taataaaggg ttatggtaca taacgatcaa tggtgcattt gtgatgctgt tgttcatgcc
     4741 agcaatcagt ctgtatccat tgatgacgct agattacttt ggtggttccg ttggacaggc
     4801 gggagcagtg gaagtagtct acgcagtcgg aatgctctta ggcggtgcac tgatcagttt
     4861 tataggaaca tggaaagacc gaatgaaacc tattattata gcctacatca ttatgggcct
     4921 gacgatcggt gcaagcggat tggttccaaa cgatagtcaa gggttcttgt actttcttat
     4981 cttgaatgca ggagcaggtt gtgcaacgcc atatttcaat acactgctga tggcaatgat
     5041 ccagcagagt tatgaatcga atgttttagg tcgtgtctta gggaacttca actctttgat
     5101 gaacctggca ggtccaattg gattattatt tgcaggacca ttagctgatc gtttaggggt
     5161 agaaaaaatg ttcttgtttt ccggaatcgg gattttatta tgcggaatcg ttttattttt
     5221 aacgtcagct gcccgaaatt atgataaaga attacagaaa aaactggtaa aagaacacca
     5281 tgagcaaaaa gacgaatagt atggaatgga gttgtgacag aagtcttgtt acaactccat
     5341 ttctgatcct aatgttcctg tggcaataag cctactcaaa tgaaaaatat gagtaattat
     5401 ctttatttgt ttaatacttg atatagggca gttttttcac gacctatcgt acttcctctt
     5461 tgcttcatag atttctttga tttgttgctc agataacgga gtgccaaatg gtagtttttc
     5521 ctttaagagt tctctttccg gtgcgtctac gcctacattg acatcgttct cgataggtac
     5581 gctgtaatga tggatatgtc catgaagatt gcggatagtg tcgttaggcc cgagcaacat
     5641 cggataatga gtcaagtaat attgttgatg gtcgaattta acgatggcac ctacatcata
     5701 aaaataaaat ttgggacttc cgtcagtaag cctaggatca tttttttcta aataacggaa
     5761 aaaagcgcga ctgtcatggt tccctttgat caagtggatt gttccttgca gttgagtgac
     5821 cagttccaaa atttctgcat ttgctttttc atattgcgga tgcatagcaa ggtcgcctag
     5881 atgataaaca tggtcggttt cttttattac tgcattccag ctagtgatca actgttggtt
     5941 catttcatcc acatttgcaa agagccgcgg agcaaagtcg ttttttccta ataagcgttc
     6001 atggaaaaag tgcatatcac tgataaagta attcatgtat cttttgcctc ccttttttct
     6061 tagagtaaac aaaatcactt atctgagcaa ctcataacat ctaaaaagaa aaggttgtaa
     6121 atgagacgtg cagctttaag cgataagaag caattttgaa aaatagtttt tcacaattta
     6181 taaaattatt ccttattgcc gaaaggctgg ccctttttat gagtatgcca attttttata
     6241 tcgaatgctc aaaagatgag tgattttttg gttgactatc atgtttgtgt actgaatagg
     6301 tccagtagaa cagtattttt acgacttgat aattaaggga tgaaattttc agaaatatga
     6361 cgtataatgt aaaatactga aaagttgcga ccttcaactc gtagatgttt cagaatctaa
     6421 ttgaaatgat ggtgatatca tgacagacaa cagcaaaaca cgcgttgtcg tagggatgag
     6481 cggcggtgtc gactcatctg taacagcact tttgttaaag gaacaaggat acgacgtgat
     6541 cggaatattc atgaaaaatt gggatgacac ggacgaaaac ggtgtatgca cggccacgga
     6601 agattataag gatgtagcga aagtagctgc tcaaatcggg attccttatt actctgtgaa
     6661 ttttgaaaaa gaatattggg atcgtgtatt tgaatatttc ctagccgaat accgcgcagg
     6721 acgtacacct aacccagacg tgatgtgcaa caaagaaatc aaattcaaag cattcttgga
     6781 ttatgcaatg gatcttggcg ccgattatgt agctactgga cattatgcgc aagtcactcg
     6841 cgatgaaaac ggcgtggtcc acatgcttcg tggagtagac aataataaag atcaaactta
     6901 cttccttagc caattatcac aagaacagct ttcaaaaacg atgttccctc taggcggcat
     6961 ggaaaaatca gaagtacggg caattgctga acgcgcaggc cttgcgactg ctaagaaaaa
     7021 agattcaacg ggaatctgct ttattggtga aaagaacttc aaacaatttt tgagcaacta
     7081 tctgccagca aaaaaaggaa atatggtcac tcttgatggt gaagtcaaag gacaacatgc
     7141 tggcttgatg tattatacga ttggtcaacg tcaaggacta gggatcggtg gcggaggaga
     7201 ttcgcaagaa ccctggtttg ttgtaggaaa agatctagcg acaaacacat tatatgtggg
     7261 gcaagggttc catcatcctg cattgtatgc cacaagcttg gatgcaagtg aaatccactt
     7321 tacgacgaat gaaccaatgc caaaagaatt caagtgtaca gcgaaattcc gttatcgtca
     7381 gcaagacgtt cctgtgacag ttcgcttatt ggatgataat cgtgcagaag tcgtattcga
     7441 cgaacctgta cgtgcaatca ctcctggaca agcagttgta ttttatgatg gaatggaatg
     7501 tctgggtggt ggtttgatcg accacgcata tcaagaaaca aaagttttac aatacgttta
     7561 agcgatatta aatgaatcaa aacagctatg gctaacaaaa gaaacacctt ttcttttgga
     7621 tagcatagct gtttttttgc aataaaatta atttttattc attttatact tgaaatactt
     7681 gtgtaaaaag ttcataatat ccaataatat gaataaagtt gctttttgtt aagcaagagc
     7741 agttgcaaaa tagaagggaa tcttttgcaa gtgatttgtt caattttata agtgagggtg
     7801 atggaaatgg aaaagaaatc gacgggtatt agcaaatggg gactggttgc tctggttgtc
     7861 agttcgtcaa tcggaagcgg tgtgtttggg atcacaagtg atctggctag ttctgcagct
     7921 ccagggccag cgatcctttc atggataatc gttgggattg gtattttagc tttggtatta
     7981 tcgctgaatc atttaggaga aaagcgtcca gatctagatg gtggtatttt cggttacgcg
     8041 gaagcttcat ttggtaaatt aggtggcttc atcagtggtt ggggttactg gttgtctgcg
     8101 tggctgggaa atgttgcttt tgcaacgatg ctgatgagcg ccatgggaga atttttcccg
     8161 atattcaaag gtggacaaaa tgttccttct attttatttg caagtatttt tatttggggt
     8221 ctgactttat tagtaaacaa tggagttgaa ggagcgagcg tcatcaatac aatcgtgacg
     8281 atctgtaaat tagtgccatt attcctattt ttaatatttg tatttatcgc atttaaagtg
     8341 aatatattca ctgctgattt ttggggtaac gtatctgata atctagtgaa tggaacaggg
     8401 cagcaaggaa cgatctggtt gcagattaaa ggttgtttga tggttatgat gtgggtcttt
     8461 gtagggatcg aaggcgcaag tgttttagcc aaccgcgcga agaaacgttc agaagcccag
     8521 caagcatcga tcattggtct gctttgttta ttgtttatct atatccttgc ttcgatcttg
     8581 ccatacggcg tcttatctca agaagagtta gcaactattt cacaacctgc tatggcaaat
     8641 attttaaaag gaatcgtggg aaattgggga gcagcgctga tcaatatcgg cttgattatt
     8701 tcgatcattg gttcttggtt gtcttggacg atgctcccag cagaaacaac gatgttgatg
     8761 gctcgagacg gtctgttgcc gaaaggttgg ggaaaaacga ataaaaaaaa tgccccaact
     8821 tattcgttag ttgttactgc aatcttgact aatctattct tattgacttt cttagttact
     8881 gattatgcct atcaatttgc ctattcttta tgtacagcgg caattttgat ttgttatcta
     8941 cttgtcggga tctatcaagt ccagttttct ttaaaagaag gaatcaaaaa acaagtagtg
     9001 atcggtggga ttgctgtagt ttttgaattg atgggcatca ttttagcagg attttcttat
     9061 gtccttttat gtagtattgc ctatcttcct ggtttttgct tctactggat cgcttgtcgt
     9121 gaaaccaaac atcaaattac gggaaaagaa aaagtaatga ttgggctgat ttgtgtggcc
     9181 gcactgcttt caatcgggtt gcttgctgta ggctggatca agatataagg gggaaagaaa
     9241 catgaaaatc gcaaatttcg gtgtagaaga atggctaaat aaatgggaaa agaaagcggt
     9301 atatgatatt gcacaaagct cgatcgaagc attgacatta gacgaactcg ttggcttaga
     9361 tgggactagc gtaagtaaat ttttcgaaaa acgcaaaaac gaaccattgg actatggctg
     9421 gatcgaagga tcagatgttt tcaaacaaga agtcgcttct ctttatcaaa ccgtcgatcc
     9481 ggaaaacatc ttacaaacga atggagcgac tggtgctaat cttttagctc tatacgcgtt
     9541 agtggaagca ggagaccatg ttgtttcgat gcttcctact tatcagcaat tatatgacat
     9601 ccccaaatct ttaggtgccg ctgttgactt tgttcatttg aaggaagaag aagattggca
     9661 gtttgattta gaacagctag aagccttagt caaacctgaa acaaaaatca tctgtttgaa
     9721 tagtgcaaac aatcctacag gcacattgct agatcggaaa actttagaaa aaatcgcaga
     9781 aattgctcgt acagtagatg catatgtatt gatagatgaa gtatatgcgc ctttaacgga
     9841 taaaggtgaa ttcttgtcga tcgtagatgt ctatgaaaaa ggtatagcaa ccaattccct
     9901 atccaaaacc tactccattc caggtattcg tattggctgg acagctactg gacctgagtt
     9961 ggcagaagtt tttcgaaagt atcgagacta cacgatgatt tgcggcggag tcctgtcaga
    10021 tgatcttgct gtacatgcat tgaagaataa agaaaaaata ttggcgcgaa acaaaaagat
    10081 cattacagag aaccttgtga ttttaaaaca atgggtagcc aatgaaccga aagttgaact
    10141 tgtcgctccc aattacgtct ctacttcttt cattaaatta gccatcgaag aagatgacca
    10201 gacattttgt atcaatttat tagaagaaac aggagtacta ttagtaccag gaagtgcctt
    10261 tgatttaccg aagcatgccc gactaggtta ctgttgtaaa aaagaaacct tggaaaaggg
    10321 attattcctc ttatctgagt tcttgaaaaa acaggcctaa acaaaaacac tgcaaggaca
    10381 gtctgtgaca actgttcaag cagtgttttt catttacgct tattgtctat gagaaggaga
    10441 gtgccctgaa gctatggaga ctcctgaagc tttttcttct gcagtttctt gctgatggat
    10501 ctcttgtgta ttgtgtcgta caagcaagaa gttgaccaaa acaagaagag aagtcgaaag
    10561 aaagacgccg cgataaccaa atgcactgga aacactggag ccgatcatag gccctactac
    10621 gttaccagtt gcttggaaag attggttata actgaagatc cgtccagcag cttgatgagg
    10681 cgaatatttt gtgatcaatg cttggacagc agggagcaga caagcatcag agataccaat
    10741 caaaaaacgt aaggcagcta attgccaaac attcgtgacg aatgccattg ggatataaac
    10801 cagaatcgca aaacccaaac caatcgcaag tattctttca ctcccaatct tgtcacctaa
    10861 gcgaccaaat cttggagcgg ctatcaaagt ggcaatccca ggtatcgaag caatgattcc
    10921 gctgatcaac gtaacattcc catgtccatg cagcaattgg cgaatgtaca gactgatgat
    10981 tggactaatg gaattgttgg atgcttggat gatcatcgtt gtgacgaaca taccgatgac
    11041 gacgtgtgga tatttcaagt ctttgaaaat ctgtttggca gaaaccatgt tttctttttt
    11101 cactgggaca aatttttcat ggacgaaaaa cagactcaac aagaagacga aaaataagat
    11161 gatccctgta atgaaaaagg tagggcgata gccaaacgct gaagcagaga tcccgccaag
    11221 aagcggtcca agtaatgttc ctgtaacaga accagttgct aaggttccaa gaacttgacc
    11281 acttttttct tttggcgtcc cagttgcgac aagggcagtg gcattactga tataaccaga
    11341 aaaaatccct tgaagcagac gcaatgcgac taattgatac acactagtca cgcaacccat
    11401 caaagaaatg acgattgcca ttcctaatga agcacgcaac agcatcaatt ttcttccttt
    11461 ttggtcggct aattttcccc accaaggaga gataagcgta gtcaccaaga aagtagatga
    11521 aaaagtcaat ccgctccaaa agttcaattg tgacgtacta taattaccta acgtatctat
    11581 gtaaagtgac ataaaaggca tgactaaact aaatccgata ccagccataa atgttccaaa
    11641 ccataagaca aacaagtttt gttcccaagt ttctcttttc ctaaataatt tttcctttat
    11701 tcttgcagcc aattagactc cttctttctg aaaattttca tctacagctc tttattataa
    11761 actacaatat gtagtttatc aatgaaagtc tcttttttct ctttcttttt cgtggtaaac
    11821 tgaaagagtg aaggatttat tttatgagag gaaacatgga aatgaaaaaa agaaatctgt
    11881 ctcaagagaa aatcatcgac tgctttcgag aactagcaga agagatggat gttcagcaaa
    11941 taacctttca gcatttagcc aaacgcttgg atatcaaggc tccctcgctg tataaccatt
    12001 tcaaaaatat tcgtgatgtc aaaacagcgc taacagcgaa acttctgcag gaacttaatg
    12061 accagctgcg acgggctctt gtgggaaaaa gtggtcagga agcactgcag atctatgcag
    12121 aaacttatcg atcctttgct ttttcaaatc aagcagtgta tgagttactg atcagtgtac
    12181 cgcataccaa tgaagctgtt ttattagatg gaatccatga aacgaatcag atcattttgc
    12241 agatatttga ggcttttcaa ttaacgaaag aagaaaaagt tcatcgaagc agggaacttc
    12301 gtagtatgat ccacggttat ctttccttac gttttttagg atactttaca aaagaaccaa
    12361 caatcgatcc agaagacagc tatttttgga tgatcaataa ctttatcgct actttgcctt
    12421 aaacaatcag attcatttgt aacaatttta agatgttcat taaatgaccc ttttataaga
    12481 gaaaattctt gcaactttct tgaaaagtga ctaagatgag tctagaaaag ctgaaagaag
    12541 gagcacgagt atgtatcaag taattaccat gttcggggac aatgagcctt ggtggttttt
    12601 tgaagactgg gaagaagata ttgaagaaga agaaacattc gagtctttcg aagaagcacg
    12661 acaagcgtac gaaaaacaat ggaacgaaat ccatcaaaat tatgaatata tcaatgcaaa
    12721 aagtaacttt ctcagcgcct tttggaatga cggagatgag cgctggtgtg aagagtgtga
    12781 cgatgattta cagcaataca agggacttgc gttgttgaaa aatcatcagc ccatcacagt
    12841 agaaagcaga aaggaatttt atgaaacaac taattctagc ggaaaaacca agcgttgcga
    12901 aagacctaag caaggtgctt ggtgctaatc aaaagcacaa aaattattat gaaggaccca
    12961 aggtgatcgt tacttgggcc cttggtcatt tattaggatt gaaaatgccg gaagatctga
    13021 ataaggaatg gcagagctgg caaatggaca cgttgccaat gatccctaaa aaactaggaa
    13081 tcaaaccact tcctaaaaca ggacatcaat taaaagcaat caagcagctg gctcagcgaa
    13141 aagatgtctc agaagcagtc attgcgacag atgccggaag agaaggggaa cttgttgctc
    13201 gctggatctt agagtatgtg catttcaata aaccagtgaa gcggttatgg atctcttctc
    13261 aaacagataa agcaatcaaa actggattca agcaactaag acctgcgaaa gaatatgatg
    13321 cattgtatga atctgctctt gcccgggcaa aagcggactg gctggtagga ttgaatgtga
    13381 ccagagcctt aactgtgaag tacgcggata atttatcagc aggccgtgtg cagacaccga
    13441 cactagcatt agtgagagac caagaaaaga aaatcgagca gttccgtcca caaacgtatt
    13501 tcacgatcac gttgcaagct ggaaaagaac aagccaaaat gcttcaaaaa aatcaatttg
    13561 ctttaaaaac acaagaagaa gcacaaaaac ttgttcaatt gatcagtaca agcaaaggaa
    13621 ctgtcacaga agtccaagaa aaattaaaaa cggaatctgc gccattgcct tatgatttaa
    13681 cagagatcca aagagaagcc aatcaacgct atggctattc tgcaaagaaa acattaagtc
    13741 ttgtacaaag tttgtatgag acccataaga tcgttactta tccgcgtaca gacagcaaat
    13801 atctgacaaa tgacatgaaa agtacgatga aagaacgatt gcaagcagtc agcgactttg
    13861 ctcctgaggt caagcagtat ttgaaaaacg gagccatcgt ccaacagaca aaagtattcc
    13921 aagacagtaa agtgaccgat caccatgcac ttttgccaac tgaaaacaga gcacgttatg
    13981 aaaaattaag taatgaagag cgaaaaatct atcaaatgat tgtctctcgt tttttaggac
    14041 tgtttgctca accccataaa gtcagccaaa caaaagttac ggttgaattt ggtaaagaac
    14101 gattcatttt tcggcaaaat cgtgtcgtcc aagcaggatg gaaaggcgaa actgaaactg
    14161 agacggaaac agtcaaatgg gaaaaaggaa tgagaatcaa tcccgatttc gccatcaaaa
    14221 aagaactatc tgcaccgcct aaaccattgt cagaagcaag cttgcttggt caaatggaaa
    14281 aatacagtct gggaacccca gctacgcgtg cagagattat cgagaaacta atcaaatctg
    14341 aactgatgga acgtacccct caaggactgc aagtatctcc taaaggtaag caattattag
    14401 tattggtaaa tccctcttta gtaacaccag atttgacaga aagctgggaa aaagaccttg
    14461 aagctattgc tgcaggaaag aaacaagcag gaaccttctt gaatggaatc gaaaaagaaa
    14521 caaaacgctt ggtgaatgag atcaaatcaa gcaaacaaga atatcaagat ttctcgatta
    14581 cccaaaagaa atgcccagaa tgtggggcca atctgcgcga gaaaaacaca cgtgacggca
    14641 aaatctatgt ctgcaccaat tcagattgca actaccgcag aagaaaagat ccaaaagtct
    14701 ctaatcatcg atgcccgcaa tgtcaccgga aaatggaaat catcgaaggt aaaaacggtg
    14761 cttatttccg ttgtaaatac gatggaacga cagaaaaatt gcttgataaa aaagagcaga
    14821 agaagaaaat gaccaaacat gaagaacgac gcttgctgaa aaaatatgca caagcagaag
    14881 aaccagaaga aagcccatta gcagcagcac taaaagcagc aatgaagaag taataagata
    14941 tgaaagaggt tgtgatagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt
    15001 gcttctcaaa tttttgtgaa atttcggctt atttccgaag aagctacttc tggaacacca
    15061 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
    15121 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
    15181 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
    15241 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
    15301 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
    15361 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
    15421 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
    15481 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
    15541 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
    15601 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
    15661 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
    15721 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
    15781 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
    15841 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
    15901 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
    15961 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
    16021 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
    16081 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
    16141 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
    16201 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
    16261 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
    16321 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
    16381 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
    16441 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
    16501 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
    16561 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
    16621 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
    16681 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
    16741 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
    16801 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
    16861 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
    16921 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
    16981 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
    17041 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
    17101 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
    17161 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
    17221 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
    17281 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
    17341 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
    17401 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
    17461 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
    17521 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
    17581 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
    17641 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
    17701 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
    17761 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
    17821 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
    17881 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
    17941 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
    18001 aaagtcttgt acgacctctt gtttcgaata aacggtgttc cagaagtagc ttctacggaa
    18061 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    18121 gaagctgacc acttctgtca caacctctta ttttgattag aacttgcgaa aataaaccgg
    18181 ttaaagtgtg tctagcgcaa gagatttgct ataatagacg ataagggtaa aatgtaacga
    18241 gaaggggttg aaaaatgtgt atcaaattgt aacagattct tgttgtgatc tgccatacca
    18301 tgtattagca aaagaaaaag ttgatttttt atcaatgaat ctccaattga atgatcaaga
    18361 gttagtagat gacctcggaa agacattcaa ttatgaccgc tacatgaatc agctgaaaaa
    18421 tggtgcgatg cctacgacat cacaaatcaa tgttggccga tatttagaat ttttccgttc
    18481 ttatgcggaa aaacagattc ctgttttata tgtggctttt tccagtggat taagcggctc
    18541 ttacagtagt gcgcttcaag cggttgaatt gctgaaggaa gaatatgaag accctgagat
    18601 ctatgttttt gatacaaaag ctgcaagtat tggggaaggg cttttagtat tggaagctgt
    18661 aagattgaaa aaagaaggta aaagcatcga agaaacccta gagtggctgg aaagtaatta
    18721 tttgaaagtc cattcatggg taacagtaga cgatctgaaa tatctagaac gaggcggtag
    18781 gatttccaaa gcaagtgcta ctcttggcag tctattgagt gtaaagccta ttattacggt
    18841 ggataaagaa ggaaggctga cgaatgttga caaagttcga ggaagaaaca agtcgatcca
    18901 gacaatcgtc aatgagacgg taaaaacaat cgttgcacca cttgatcaaa tcgtttatgt
    18961 ggcttacgct ggggatctag agagcgcgga gaaggcaaaa cggcttttgg aagagcagat
    19021 aaagatgaaa gatgtgaagc tttatccctt aggaccaacg atcgccagtc acacaggcta
    19081 tggctgtatc gcgatttttt cgatgggaat cagtcgataa aagcagtaac tcattctgta
    19141 aaaaagccca gaaattgatt gtaacaaaaa cagcgactct taaaagatag atgagcatcc
    19201 tatctcttag aggtcgctgt ttttttattt tttaagctaa ataggccatt atgcccataa
    19261 ctcgttcaat tgtttttgaa tatctgcatt ttccaaaaat tcatcatatg tcgtatcagc
    19321 tcgatcgatc acacctttat ccgaaacggc aatgatgcgg tttgccaatg tttggataaa
    19381 ttgatggtca tgtgacgcaa ataagattga gccagtaaat gccatcaagc cgtcattcaa
    19441 tgctgtgatt gattctaagt ctaaatggtt cgttggatca tctaatacaa gtacgttagc
    19501 tttcgaaagc atcatttttg aaagcatgca acggactttc tctccaccag ataaaacatt
    19561 cactggtttc aatacttctt caccagaaaa caacattctt cctaagaagc ttcttaagaa
    19621 ggtgttatca tcttcttctt tacttgcata ttgacgcaac cagtctaaaa tcgttagatc
    19681 agtatcgaat tctttcgttg tatcctttgg taaataagaa ttacttgttg tcacacccca
    19741 acgtactgtt ccagtatctg gcgtaatttc acccatgata actttgaata gcgttgttgt
    19801 tgttatatca ttttctgcga cgaaagcgac cttgtcttct ttgctcaaat taaaagatat
    19861 atcatctaaa attttctttc catcaatcgt tacacttaca ttttctacct gcaataagtc
    19921 gtttccaatc tcacgttcag gtttgaatcc aacgaatgga taacgtctag aagaaggttg
    19981 gatatcgtct aaagtaattt tttcaagcat ttttttacgt gaagttgctt gttttgattt
    20041 tgatgcattc gcactaaagc gggcaataaa ttcttggagt tctttgattt gttcttcttt
    20101 tttggcattt gcgtttgctt gaagtttagc cgctagttga ctagattcca accaaaaatc
    20161 ataatttcct acataaagtt tgattttacc aaaatctaga tccgccatat gtgtacatac
    20221 tttgttcaaa aagtgacggt catgggatac aacgatcaca gtattttcaa aattgatcaa
    20281 aaattcttcc agccaattga tcgattggat atccagaccg tttgttggtt cgtctagcaa
    20341 aagaacatca ggtttaccaa atagtgtttg tgctaataac acttttacct tttgtccacc
    20401 agttaattca ctcatttgct gatggtgcaa ttcttcaggg atgttcagac cttgcagtaa
    20461 aacagctgct tctggttcag cttcccatcc attaagctca gcaaattctc cttctaattc
    20521 agctgctcga ataccatctt cgtcagtgaa atcctctttc atgtaaatcg catctttttc
    20581 tttcagcact tcatacagac gcttgtgtcc catgatgact gtttcaagta cagtatagtc
    20641 ttcatagtcg taatggttct gtttcaatac ggccatccgt tcatctggtc ccatagtaac
    20701 ggaacctgtg gaaggctgca gatcccctga taacacttta aggaacgttg atttacctgc
    20761 accatttgca ccaatcaagc catagcagtt tccgggggta aatttaatat tcacatcatc
    20821 aaatagtttg cggtctgaaa agtgcaaact cacatcattt acagtaatca atttttttcc
    20881 tcacttttat cattattagt tttgacaagt cttatagaat tatatagggg aaaggtaaaa
    20941 gattcaaggc aatcgaacat tctttgtgtt tcacgtgaaa cagaatgaaa aaaataaaaa
    21001 taaaagcgga gcgaaaatag ctactatagg atttattagt ttgcaacagc ataatttttg
    21061 acgttattta taagagggag tactataaaa ctgtaaaaga aatgaaagaa ggattgaaaa
    21121 tggatgaaaa aagaaccatc gactggggct cattgatttt gggaatttta tttgtacttg
    21181 tctcattgtt atcatttcaa gaccctgtcg gaaatttagt tgcaattgta gtagtctttg
    21241 cgatttttgc ctttattaaa gggatatttg aactgtttgt acggaacaga ttaaaagagc
    21301 taacaggata taaaggaaag atgcccttaa taatcgggat catcgatatt ctagttggcg
    21361 tctttttctt attcaatatt ggtgcaggag tcgcagcatt gccttttgtt tttgcagtat
    21421 ggtttattgc tgattcagtt ttagcgttat ttactgctga cttggcaaga ggagtaagtg
    21481 aaggatatta ttggtttacg atcatcgtca atattttagg aattttatta ggaattttct
    21541 tattgtttaa tccaatttct tctgcgttga cattaagctt tttagttgga ttctatttta
    21601 tgttatttgg tatcacacat atcgtctatg catttagata agtaaaatca aacaaataaa
    21661 aaagccggct tccgattaca gttttactgt tgtaattgga agccggcttt tttatcatgg
    21721 acaaatatgc acgaggtgat ttttaggtgt cgagacaaat ttatgcaaaa atgtgagaag
    21781 ctatatacac aaattttatc ttaatactcg aagcatagcg cagttgtcac aacctctttt
    21841 catataattc attcaatact ggcaaatcat ggacttcata tttttctttg aatgcttgga
    21901 tttcttcttc agaatatttt cttggatcta aatgcagatc ttctactggc aatgggcgct
    21961 tgttattgat cttcacatcg attactacag gacgatctga ttttgcagct gcatcaaagg
    22021 caaatcgaag ttgcgagtat tctgtgactg taaagctatt tgccccgagc gcttctccaa
    22081 ctttaccgaa atctgcatct tctaaaaata caccaaattt cttttgttcc gtatcttctt
    22141 gttctgcttc gatgaaacca aaagaatcat tcgaaaagac aacattaata attggaagct
    22201 gatatttgac ttgtgtaatg atatcttgca tgaccatagc aaacccgcca tcaccactta
    22261 aggtaaatac ttgtttttct gggtagctta gttgtgcggc aattcctcct ggcacaccat
    22321 tacccatagt tgcgaaccat ccagatgtcg tatgtttttg tttaccgttc atattcaata
    22381 aacgaataga atggatagtc gtgttaccga catctgtgac gaaaatcgca tcgtcttcag
    22441 caatacgatt gatttcttta tagacagcct ctggacgaat cggttcttcc tctcgatctt
    22501 caaaagattt caaccaattt acccattttt ctttattttc ttgatttgct aggtaccagc
    22561 tatcagcagg gcgagcatcc cctaactcga caagtctttg caaagctgtg tttgcatcac
    22621 cgaggatcga caaagatgtt tcatggcgtc gtccaaattt tgacgcatcg atatcgattt
    22681 ggataaattc agcagttgga ttaaaaaatg cacgtccaaa tgggaagtcg ctaccgacaa
    22741 ataagatcag atcggcttct gccaatgctt catttccggg tttagtagcg acacgtccag
    22801 caaaaccaag aaagttttca taatcatcag gaataattcc ttttgctaga acggaagcga
    22861 caacaggaac tgaaaaatgt tctgagaatt ctttgatttg cggaaatccg tttcgcgttc
    22921 cttgaccaac ataaagaact ggtttttttg ctttttcaag ataaggcaaa gcagctagca
    22981 aatctttttc ttctggtaat atcacacttg ttttgtgagt gtgagcggtg gagaaaggtt
    23041 gttcgtcaat ttcttcaaaa ccaagatcaa cagggatagt cacgatagcc acgcctttat
    23101 gctcataggc ggctttaatg gcttcatcta ctacatgcgg aagactttgt ggtgtcatga
    23161 ctgtacgatt gtatacgcta acatctgcga aaataggatt ttcatttagt tcctggaaag
    23221 agttatagtt catggatgta gaagcaactt gtcctaagaa cgctactaga ggcacatggt
    23281 ccatctgtgc atcatacaaa ccattgatca aatgtgtcgc accaggtcct gcagagccga
    23341 agacagcgcc gactttcccc gttaattttg catctgcagc ggctgcgagt gcgccgactt
    23401 cttcatgacg aacttgaatg tagttgattt cggttttttc ttgatatagc gcatccataa
    23461 ttgaattgaa agatcctccg ggaatgccgt aaatatggtc gataccccag ctttcaagaa
    23521 cttttaccat tgcgattcct gcattgattt ttgacatggt gtttcactcc tttagcaatg
    23581 atgatacata aattttttgt atctagagta tagaactttt tatcgaaaat cccaactaat
    23641 tcgtcttttc acaaaaatgt agtcatgttt ttgggaatat gctagagtag tacatagata
    23701 gattcaagag aggggaatgg catggttgaa tggctattga gacttgaacc ttggcagcaa
    23761 gcacttgctg ggacagcttt tacttatttt atgacagctc ttggtgccgg gttggttttt
    23821 ttctttaaag aaatcaaaaa agaagtgcta aacttgatgt taggatttgc ttctggtgtc
    23881 atgattgctg caagtttttg gtctttgtta gatccggcta ttacaaaagc agaagaaaat
    23941 ggagatattg cttggctagt tgtaagtatt gggtttggtt taggcggtgt gtttttatat
    24001 atggcagata aaacactgcc ccatatgcat tttggaccac aacatgaagc agaaggattg
    24061 cctacccatt taaaacggac gatcttgctt gttttttcta ttacattgca taacattcct
    24121 gaaggtttag ccgtgggggt agcattcggt gcagcagcaa ctgcggataa tccgacagcc
    24181 gctatattag ccgctgtttc ggtggcgcta ggtattggta tccaaaactt cccagaagga
    24241 gcagctgtat caatcccgtt gcgacaagaa ggtctgagtc gtaaaaaggc ctttgtttac
    24301 ggacaagctt ctggaatcgt tgaaccaatt gctggagtga ttggagcgct actagtaacg
    24361 aaagtggagc tgctgcttcc ttatgcactt gcttttgccg caggagcaat gatttatgtc
    24421 gtggtggaag aattgatccc tgaagcacag cagacattgt ccagcaaaca tcattttgca
    24481 gtttttggcg tcatgagtgg atttatcatt atgatgattt tggatgtggc attaggataa
    24541 ataaaaaaaa tatatatgat atatcaaata gggccgcgaa cttatatcgc gaccctattt
    24601 ttttagtaag ttatatcata atgaagtgaa tggtatcata cattaataat agttttttct
    24661 atgtgtattt cctttgatcc agcgaacaag ccagatgata agtgcgattg ggattagaac
    24721 tttgaccgca aaccataaaa gcccgcttag gacactgaca aaaagactga tgacgaagct
    24781 agctgcaatt agcactaaaa gaaccatgaa gatatttaag aacatatttt tttgacgcat
    24841 acaaaaccct cctcttgttc attctttact atagaaaaag tataacaggg attgtttaaa
    24901 aacacctcca actgaagact gatttttact tcatctactt aaatgatcaa acaattgctt
    24961 tttctctggt ttatcatcta aaatagagcg gaaaaggagg agaatatcat gcctaaaaat
    25021 cgtcttgaag catttacaga tgctgtaatc gcgattatca tgactatttt agttttagaa
    25081 cttcatgaac ctaacggaga cacgtgggag gcgctcgctg cattaggtta ccgatttttt
    25141 atctatattg tcagttttat cattttagcg atttattgga acaatcatca tcatttattt
    25201 caagttgtac ataagataaa tggacgggtc ttatgggcga acagtttctt tgtttttgcg
    25261 ctttcgcttt ttccgtttgc aacttcatgg atcagtgagt acatcaatag cctggctcca
    25321 gaaatgactt atggttttgt taccttgacc gctaatattg cgtattatat cttggcaaaa
    25381 gaacttgtca aagcaaataa aggccacgag aaagcattga aggcgatgtt gaataattac
    25441 aagaaatcgt atctatcgat tggtttgaat gtgttaggaa ttgtcttagg gtatttagta
    25501 gcgccagtag ccgtgctgat cgcaaacatc gtgattctag ctgcttggat catcccaagt
    25561 cgtaccatcg aatcacaata tcggtaaaat gatccgtgtt taaaatttaa gagaaaaata
    25621 tagaaaaaat aaataaacga tcaaatatcc gagtgatact agtattcaaa gagggatgca
    25681 gatggatcaa aaaagaattc atgtaggact gattactagt tttgtcacga tgcttattta
    25741 tttggatcaa ggtcatttga cttggacagt agttggtgtc gtaggatttt tgacattatt
    25801 cacagcaata aaaaaataaa catctttatg taatctgatg ttctatctat ttaagaaaag
    25861 catgtttaca gcagtatgat tctgttgtaa acgtgttttt atttgtggaa aatttaattt
    25921 ttagaaaagt gtatcgaaaa atagatagtt agtttttcca cagtggataa cttcgtaaga
    25981 gataaaaata aaaaattagt caaagaaggg cgtatagatt tcttttgata ggagatatac
    26041 aacagaaatg aataaatatt aacgtgtttt caaacagtat aaaacggtgt ggtttttgga
    26101 ataaaaaaat aacatatctc attttacact tttgttattt tatacttaaa aaataacgac
    26161 acagaaaata ccgtgtctca gatttctcta ctacttctaa aaactactgt ttttttcctt
    26221 tgaaatgcct tcaaaaagta ggatcaggca gaatatactg gcagaatcaa ggagaaggga
    26281 gcctaaataa ggagaaaaac catagaaaat ataaagtaat tacttataaa acaagctttt
    26341 tatagaaaaa taatatacct gagtagggta taaattaaga cggaattcct ttctagattg
    26401 agatattgac taggaagaat gttaagattt taagccttta agctaaaaaa aaaccctttt
    26461 tgttaaataa ggggaatata gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa
    26521 tatagtagat tagagatgaa aatagatttt tttaaaccaa tgactgaata aaaagaccgt
    26581 catagataca cattggaatg tgtctattac ggtctttttt tacgaacgat tttattgaag
    26641 acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga aataagaata aaatgtatcc
    26701 caaaataatg gctagacggt ctaacttatt tgtcgataga tgtttgttta gaagttgata
    26761 ggcccataaa cctatcagtc ctcctgttgt attagttatc aaatcagtga tgtccgctac
    26821 gccaataccg aataggaatt gaatcaattc tgccaagaag cttacaagga aaataatccc
    26881 taatttttta gaaaaagaaa gtctcttgaa attgacattc aaaagcaccc caaaaggaaa
    26941 aaagacaatc aagttataag tggtctcacg taataccgtc gtattttcaa aagaaaaagt
    27001 agaaaaaggg acgagattga cattgctgta agaataagtg agaaattgtg gaagattaaa
    27061 tgatagcttg aataaaatca accataatag aaagaataaa taaatcccta ataaaaattt
    27121 tgatacatac cgcatagaca tctcctccat ttaaaaagta taccatcgaa aaccgctgaa
    27181 aaatcataaa aaagtatgaa gtttaaggac caacgtagtt atcgtacgaa aattagaagc
    27241 ttttttttgt tgggaatccg gtttctttaa caaagctaag atagcgagtt gcttggaaaa
    27301 gttgctatct tttttctgtc aaaacgactc ggaatgttcg ttttttgttc taatttctcc
    27361 aagttgttta ctttttgata ctaagtttat gaaaaatcaa gaaatagttg ttatactagg
    27421 taatgctgac aaacgggatt ggcatttcat cttggttcag ctttttttcc aattactagg
    27481 aaggagggat agattgttca agacttattt tcgtttcaat aaaatacaag ataatccttt
    27541 tcgtatcatg aatctgatgc tacttatctt tattgtatta tggttaggtt acataaatca
    27601 tgattttttg atttcaagtt tttcatcatt agggatcttt acttttttct attatcaaaa
    27661 cataccgatg aaatatttgc tgaaacgtat gtttctgatc ggttgcggac tgttggttgc
    27721 tttcgttttg gggatcttat cgacttatgt tttatggctt gaaccatttg ccgtagcttt
    27781 ggttgctttt tccagcagat tcgtgttgcg attattccat atttctaaac caggtggctt
    27841 gttttttgcg atgctttctg ccatgggaac gagcatgcag ctgccaatcg ctcaattacc
    27901 gatcgtctca ctttattttt ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac
    27961 aaaattactg gattcacggc ctgagcagac aattgaaaaa gcaactttaa aagaaagatt
    28021 tcacgaagag cctttagtga tcattgattc tgttttctac agcgctgcct tatttctttc
    28081 cgtttatgtc agtcacggct tgaatctgca taatccttat tggttgacct tgtcatgtgc
    28141 atcgattttg ctagcagaaa acttggatgc gatgaagcat cgacaagtcc aatatctgat
    28201 tggatcgatg ggtgggttat gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt
    28261 gcagaccatt tttctgatca cttttctata tggaatcgca caatttctag ttgctagaaa
    28321 ttatgcagtt gctaatattt ttcttaatcc gatggcgttg atgctatcta cacttattcg
    28381 tggagcatac ttgatttctt tgatcgaata tcgtttttta gggatcgtca taggaagttt
    28441 catcggttta ggtgttgcgt gggtaatgac ggttggtttg caacattatt taacggttgt
    28501 tcgtcaaaaa ttagaataga aggacaaata tatgtgccaa aagaggttat gacaagattt
    28561 ttgtcacaac ctctaattca aggtgttcaa aagccagatt ctcagcatta agtcaaattc
    28621 acacaaaaat gtggaaagga actttcaaaa aaattttata tttattctat caaaaaattt
    28681 tgtttatgat ggtaactgaa taaactttcc tgagatggtc aatgctttcc ttgaaagtct
    28741 tacagatatt ctttcttcat agaaagttta agttttatag ttagtgtaca ataaaagtat
    28801 gtttatacca tatggtgtcc ggataatcag tcatttaaac aatgaggttc aaagatatga
    28861 aaaggggaaa tttgatgaag aaaataagca atgaaatcca aattctggca gaaactttaa
    28921 ctcttcccaa agtcgttata gagcaaatga ttgtacaagc aaaaaacatc cttgatgagg
    28981 tgtttgagca atatagtcat gaactaatgg atcctaataa agcaaaggcg actgcaaaga
    29041 aaatagacaa agtctatgaa gaggaacctt ttgcagcatt agctatttat ctatatgcag
    29101 cttctcaaag ttggaaaaaa atttatcaac cattgaagat tccaagaact atctatttgg
    29161 caacaatgaa tgcttttact agatttattc atgaacattt ccaagtaaca ggaagctatc
    29221 gctttgaccg tggtttttgg atttggcatt acactagtgg cttgatcttt cggattggtg
    29281 aattggagta cgaaatgaca tattttccga aagggtacac ggatctaaga ttagaaggaa
    29341 agcagtgcct ttctcttcat attccttccg atgcagattt atctcagaat aagataagag
    29401 atagttacga aagtgccatt cgtttttttg aactttattt tcctgattac cattaccaag
    29461 tgatgtacac agatacttgg ttactatcac ccaatcttac taaatggttg aaaaaagagt
    29521 caaaaatctg tctttttgca gccgattatc gattattatc agttgatgaa caggacgata
    29581 gtggagtccc ttggatcttt ggacgagtag atgcccaaat acatgattat ccagaaagta
    29641 cgagtttaca gcgacaagca aaagagcaat tgcttgcagg cgagcacatt ggctctggct
    29701 taggtattct tacttcataa gtttagtact tcgaaatagt ttaaaagagc gtgagacaaa
    29761 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
    29821 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
    29881 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttagtgttct atcgaacaca
    29941 cagtacgttc aataggtaaa ctagctataa caatagatat taaagttaac ggacaagagg
    30001 gcctttttta ggtagctaaa acgtaataat gaaaatttat ttccagagat aggagtcaat
    30061 cagctagtct gatcgactgt tttaacaagt tgactttcat tttaactttt gataccattt
    30121 aaaacacaaa tcgtaattgt ttcgatttgt gttttttagc atgactacaa tcaaagaaga
    30181 aagtgggtcg tccatatgga aaaaacagat atctcaagtg cataccgccg cttgaaaagc
    30241 ccaaatatca aaacacgaaa acgtgcgctg aagatcatca aagatgtgaa acggaatagt
    30301 gggaaaaggt aagaagaagg aaacttaatt aaaaaaatag ccgaaaaacg tttttcggct
    30361 attttcaata atcggttttt gtgtaaggtt tccgaaagaa ctgctgttga ttttccattt
    30421 ttaacatatg ataaattggt agaatctttc ttttcaaatt gataccattc cctaattcca
    30481 gttatatagt taactacaga accacaaagc acgtacgttt ataaaagtat tatctgtagt
    30541 tatttttcaa ttccctttca acctcttatt ttgaccagta gtattgatca ctttaccttt
    30601 taacaccttt gttcgttttt gaaacatttt tagtcaagaa tggaaacgta actacataat
    30661 caaaggtttt gcgaaatatt tcatgaaata tttcatgtag aagcgagcaa tacggttgca
    30721 aataaccatt atcgtttaat aagcagttac gaaagctccc aatatctcta acgttcagct
    30781 cactccattt cttttcttca aaaaaatcag tgattcgtat ctctttctat ttgtcgaaaa
    30841 agttaacttt aaaaagtaac gccgaaaaaa tatgcaaaaa aataaaaaag cagttactat
    30901 tgctagaata acatcaagga ataataagtt ttggttttca gataatagtc gataagttgt
    30961 gtattggaat tttagataca gtaataaagt atttttcatg aaattttgtg agaatcacta
    31021 ttacaagtat ctttaataaa attaatggtt aaggaggaac ttaaagtaga atgtaaatga
    31081 atagttgtag tcagtcgatt gtgatactag actcaaagtt gcaggaataa gtttttcaat
    31141 ttgtgattgg aagataaaaa atgaaaaaga agggcttttt tttatacttt gttgattgta
    31201 gtgtttattt tccctctgtt tagccaagta acattttcta tgactcaacc agtttcaagt
    31261 gaggaacatg ataacctttt tcgtgctaca gaagagttta agaaatatca ggaaaatatt
    31321 gttccggaga ctccattagc tagtgagcca attactgata ttgatggaaa ccaaatagga
    31381 tggtatatac agtacgaagt tatggtggta aataatgact caatagtaaa aaatacagta
    31441 ggcttagagc taacaccgat tgaaaaggtg agttcattgt taacgtatat ttatactgat
    31501 gaaagtgacg agactgagat cattttaacg gattactcaa aaattgctac tgataaaaag
    31561 tatatattgt ggatgtagac actagtgaaa tactttatga tatagaaatt gatgaaaatt
    31621 tactagatgt agcacaagaa atcgatcatc aaaagcagga agctattcga tatgctggta
    31681 gaaaagggaa aagaaaatat ttattacgtg aatgtagcaa aagttagaga agatgaaaat
    31741 gagtggaaag aattcaagag tcgctactca attaactcga ctccgacatt tacagtttac
    31801 agagaaggat cgatagaaaa aactgttttt tggactaaag aaagtggaat gtctttagct
    31861 gaagtagaag agtttttgga ttatgttagt atgcaacagt aagggagttc ttattatgaa
    31921 aaattttgac acatattatg ttattgtttt ttcgatatct ttgctgttaa tattactaac
    31981 tttaatgcag tataaagagt atccagagta ttttatttta acttttgtat atttagtaag
    32041 tagctacaca cttctatata aattcagaag ttcttctaga gtttctaaag tttgtttctt
    32101 aattagctta ttgattctat aatatttttg atttatatag gaaattaaaa atattataga
    32161 atcaacactc agagaacggg ttggttttta atagatttaa taagtactga taatgaaatt
    32221 tctcctggtt gggaaaacga agttggttac cctgtagaag aattttttca agattacttt
    32281 gcggagtatg ccgataggat aatgagagat tacgtaacag ataaaactca agaagtcatc
    32341 aatcaatccc agtcattaaa tgaattatct gataaggcga aagaagaagc agttgccatt
    32401 atttcttcag ggattagtga ctatattgat atgagtggtg aaatcaatta tggcgataaa
    32461 gacaaaattg tacgcatgat aaaagctttt atcaaaactt ttgatgtgaa ggcagatctt
    32521 aagtttaatg atacttacct agtaggaaaa ttaattaata tccttgggac agagaaagat
    32581 acggaaacgt ttatttcctc tttgtctatg ttgtttacag ataagcattt ttcaactcag
    32641 cttggtggag aggaactagt ggaaatcttt taaccattac gcccagcatt aataaaagta
    32701 tatcaaaaaa gtactcctga tgatttttaa gattggtttg agaaataatg tgaaaacact
    32761 atttttaatg ttaattatag atagagatga acgcgtcata tggttaaaag aatgaaaaag
    32821 tcaaagatac actgctatat ttcactaagg atatagcagt gtatctttca tgtcgtttca
    32881 ctggcagttt tttataaaag gaggtgtgca tttcagttgc ttttctttgg tagttagttg
    32941 aatcgtttaa gaattaacgg atgtggcaga caggtgctct gtccaaaaac cagtaaaatc
    33001 tttgatggtt ccatctttat ttagctgtgc ctgttcaaat actgttttgt ttttaccgaa
    33061 tggatcaatt gcaataattg ttccgatccc cgcacgtttg gcagcaagta atcctgagta
    33121 agcatcttcg ataacaagac aatcagccgg agctaagctc agtttgtctg ccgctttaag
    33181 aaaaatatcc ggttgcggtt tcccagggaa agagccgtcg tcatagacaa tcttgtccca
    33241 gtcaaaccag cgggctaaat cgaaaatatc aaaataaaaa gcaacattct ctttgacagt
    33301 agccgtggca atcgttaatg gaatctgttg gtcagtcagc tggtcaagag tcgcttcaag
    33361 cccttttgtc aactgaagct cgttttcatt ttgcaagcaa agcatacgat aatagctttc
    33421 tttttcttct gacaatacgg cgatctcaga ggcggtcaaa tgctctgaaa tgaaatgttt
    33481 caaaatctcc gtattcgtcc gaccatgaat attttttaaa atatcctcgt ctgataaacc
    33541 atcttttgta tgatcatgta tcatatgcag ccaagcagct tcatgcagat gagagtccaa
    33601 aaacattgtt ccgttgaaat caaatataat ccctttcata gttacgctcc tatagtggta
    33661 ttttcggttg aatcagcaat ttgcagatcg acatattgtg catagtgctc ttgaacctcc
    33721 cgcgagatct ggcgatccgt aatgagcaga tcgaaatcat gcaaattata aaacacataa
    33781 aaatcatctc gattgaattt tttattatca acgagtagat atttcttacg ggaattattt
    33841 agagcgatct gctgtgcttc tccttcttcg gtactgtaag tagagatcgc ttcgttgtga
    33901 acgccatttg aactgataaa tgcttttgta aactttagtc ttcttaaatt gtcgttggtg
    33961 atcggtccga caaatgcgcc ggtatttttc cggaaatcac cgccaatcag tagaatttct
    34021 ggtccatctg cttgagcaag aatatcaaaa actggataat tatttgtaat gatccgaatt
    34081 ttcttgttca gtaaatgcgt cgccaatagt tcgattgttg tacctggtcc taagaaaatc
    34141 gtttctccct cgtgaatcaa ggaggctgct gtggcagcaa tttctttttt ttcttgaatc
    34201 tgcagagtgg ctttttcagt gtgagaaagt tcttgatcta aagtgtagga aagactttga
    34261 gcccctccat ggacacgcag cagcttgccc gatttttcta attcatctaa atctctgcga
    34321 acggtcatat cagaaacatc taattgattg atgatttcat taacggtaag gattcctttt
    34381 ttattgacca tttctacgat agtaagcaat ctttcttttt tcaacattgt cttcacctct
    34441 aatttaagga taatctatca tataaacgga caacagtcaa tctaattcga acaaaagtaa
    34501 acatttaggg tggtttttat gtttgttaca atagatttta ttgctgtttt ctggttttta
    34561 tgattatttg tgtttgtttt tgttgacaaa tgttttggat gcgtttacaa tgcaatcagt
    34621 tagaaagagc gaaaactgat tatagatttg aggagagatg tacatgctta aattaccaga
    34681 agattttatt tttggcggag ccacagcagc ctatcaggtt gaaggagcca ctaaggaagg
    34741 tggcaaaggc gctgttgctt gggatgactt tttagaggaa caagggcgat tcagtcccga
    34801 tccggcaagt gatttttatc atcaatatgc aaaggacatt gaattgtgtg aacgttttgg
    34861 tgtaaatggt ctaagattat ccattgcttg gagccgcatt tttcctgatg gagctgggaa
    34921 accaaatcca gaaggaatcg ctttttatca tcgtgtgttc gaagaatgta agaaaagaaa
    34981 cgtaacgccg tttgtgacgt tgcatcattt tgatacccct aaacgtttgt ttgaccatgg
    35041 agattttttg aatcgagaaa caatagaagc atttgtttcc tatgccattt tttgttttca
    35101 tgaattcaaa gaagtcaagg tgtggagtac ctttaatgaa atctatccag ttgcaaccaa
    35161 tcaatatttg ttaggtgttt tcccaccagg aatcaaatat gattttacaa agatcgtcgc
    35221 ctgtcttcat aatatgatgg ttgctcatgc ccgtgtagtg aactatttca aagaaaacga
    35281 acttccgggt gaaattggtg tagttcactc attagagacg aaatatgcgg caacagacgc
    35341 gccggaagat aagcatgctg cctttttaga cgatgcattg tcgatccgtt tcttattaga
    35401 tgctacctat ttaggctatt attcaactga aaccttgact gctcttgatg agatatgtga
    35461 agcaaatcag gccagctatc attttcctga agaagatttc gttgaattga aaaaagcatc
    35521 cacgcgtaat gactacttag ggatcaatca ctaccaatgc cattttgtca aagcttacga
    35581 tggagaaaat gcaatccatc ataacggtac gggtgaaaaa gggacctctg tttataaagt
    35641 gaagggaatc ggagaacgta tttataaaga aggaattcct cgaactgatt gggactggct
    35701 gatttatcca gaaggtctat atgacttgtt gctgcgaatt aaaagcgatt acccacatta
    35761 caacaaaatc tatatcactg agaacgggat ggggtataaa gatcagtttg aagatggcat
    35821 tattatggat cagccgagaa tcgactattt gagagtctat ttggaatcct taagcaaggc
    35881 gataacagcc ggggtaaatg tgaaggggta ttttttgtgg tcgcttatgg atcttttctc
    35941 atggacaaat gggtataaca aaaggtatgg cttgttttat gttgattttg aaacccagaa
    36001 gcgttaccca aaagaatcag cttactggta caagttggtc agtgaaacca aaacgattat
    36061 ttaataagtg ataatggagg aagaaaagat gaataaagag gaattacaaa tgcttggttt
    36121 tgagatcgtt gcatattcag gcgacgctcg cagtacatta ttgaaattat tgaaagaagt
    36181 aagacaagga aactttgaac atgttgattc ggcgttgaaa gaagccgacg aaaatttaac
    36241 ccttgcacat aattcgcaaa caaaaatttt agcggaagaa gcatctggga aagagatgga
    36301 gatgggattc atttttatcc atgggcagga tcatttaatg accactttat tgctgagaga
    36361 tctgattcaa gactttattg tactgtacag acagaatcaa ggataggaga agaacgctat
    36421 ggatggaatc attaaacaaa tcgaaaaagg gaaaccattt tttgaaaaaa tctcacgtaa
    36481 tagttattta ggagccgttc gtgacggatt tttaacagcg atgccagcta ttttattttc
    36541 aagcattttc attttgattg ctgcggttcc tgagattttt ggttaccaat ggcctgaatc
    36601 tgtctccaca tggctatgga aagtctataa ttattcgatg ggtgttgtag ggttactcgt
    36661 ttcagcaacc actgctcgct gcttggctga ttcgatgaat cgtcgcttac cgaaaaacaa
    36721 agtaatcaac accacctctg tgatgctggc ctctgtggta agtttcctgt tgttaagcgt
    36781 ctctccgatt gaaggtggat tttcaacgga atatatgggg acaaagggac tactggcttc
    36841 ctttgtcgct gcatttttaa cagttaattt atataagttt tgtgtgctga aagatattac
    36901 cattaaaatg cctaaagaag ttcctggaac gatttcgcaa accttccgag atatttttcc
    36961 tttctctttt tcagtatttg cggccgtttt aattgattta ggcattcgac aaatcttcca
    37021 aatctcattt gcagaagcaa tcattacggt gctgcagccg ctatttacag cagctgacgg
    37081 gtacttagga atcgctatta tctggggagc gatggcgctg ttttggtttg tcggtgttca
    37141 tggaccgtcg atcgtggagc cggcaattgc ggcaattatc tatgccaatg tggaaaccaa
    37201 tctgcaattg ttcaattccg gtgaacaagc ttctaaagta ctgacggtgg gtttagggaa
    37261 ttttgtagga actatgggag gaactggggc aacattggtt gttcctttta tcttcctact
    37321 ctttgctaag tctaagcagt tgaaggcagt tgggaaagct tcctttattc cagtaagttt
    37381 cgctgtcaac gaaccattgc tatttgcggc acccatgatt ttgaaccctt atttttttat
    37441 tccatttttg ttaacacctg ttgtaaacgt ttgcctgttc aagttttttg tcgatgtatt
    37501 gggcatgaac agctttatgt acgttttgcc ttgggcaact ccagcaccga tcggtttggt
    37561 tctaggaacg ggcatgggta tcttagcttt tgttctagca gcggttctaa tcgtcgttga
    37621 cttcctgatc tatctaccat tttgcaaagc atacgatgct gtcttagtag agaaagaagc
    37681 acaaaaagca gaggtgatcg caaccgagga aggtgcttct gccgatgtac gtgaggaagt
    37741 acctgtggca gcgagtcaag ttatcagcga tgcaaataaa gaaatcaatg tgttggtact
    37801 ttgtgctggc gcagggacaa gtgcgatgtt agctaatgct ttaaaaaaag gtgcatctga
    37861 atcgaaagca ccgatcagtg caatggcggg tgcttatggt tctcattacg aaatgctgca
    37921 ggattttgat atggtggtct tagcaccgca agtcaattct tactatgatg atatcaaaga
    37981 agacaccgat cgcttaggaa tcaagctggt tgcgacaaaa ggagcagaat atatcggatt
    38041 aacgagagat cctgaaaaag ctgttcagtt tattctgaac caatttcaat aaaggaggag
    38101 ccataatgaa aatgggaatt tggttgcgta aagctgtttt atttgcagct gtgttagggg
    38161 ctttgggggc tgtttcaaaa actgttgaag catctgccag cgaatttatt cgcggagcag
    38221 atatttcgat cctgtcagat atggaaaaat ctggtgcaaa atattatgaa gacgatattt
    38281 caaaagatgc cttagagatt ttaaaaaata atggtgtcaa ctatgttcga ctgcgtttgt
    38341 ggcaggatcc ttataatagt aatggagaaa gttatggagc agggacaaat gatctggaaa
    38401 cgacgattga ccttgcaaaa cgagcaaaaa atctgggact taaggtgctg ctggattttc
    38461 actacagtga tttttgggtt gatcctggga aacagaatct tcctaaagca tggcaaggtc
    38521 tgacctttga agaaatgaat acggcattgt acgactatac aaagaacgtt ctgtcggaaa
    38581 tgaagcagct ggacgtttat cctgatatgg ttcagatcgg caatgaactg aatagcggaa
    38641 tgctctggcc ttatggcaag tcttggggag aaggcggggg agaatttgat cgattggctg
    38701 cttttttgaa gtctggtatc caagctgttc gagacactca acctaaaaca actccagtaa
    38761 tgctccactt agctgatggt ggcgatacag gagcatttac ttggtggttt gatgaaatca
    38821 cgagtcgagg tgtgtcattt gatttaattg gagtgtcgta ttatccgtat tggcacggtt
    38881 caatgaatga acttcaaacc aatatgaatg cgatcagcca acggtatggt aaagaagtga
    38941 tcgtagttga aacagcttat gggcatacaa cagcgaatgc cgatactatg ccaaatgctt
    39001 ttggtgaagc cgaagcagct gcggggggat atgaaccaac accgacagga caagcagagt
    39061 atttgctgga tcttgccgat cggattcagg cagtaccgaa taatcgcggg gcgggcttct
    39121 tctattggga acctctttgg tacaacggaa atgtgagttg ggcaacacaa gcaggtatga
    39181 attatctcag tgttcaatcc actatgggaa acgaatggga caaccaagcg atgtttgatt
    39241 ttgctggaaa cgccttgcca agcttgcgtg cgtttaaaca agcaggggcg caaataaatc
    39301 tagttaaaaa tgcaagtttc gaaagcgatg gaccaacgac cagtccttcc aattggcaaa
    39361 cctggtttca acaaggaacc gctaagggta cgatcaaaac ggaagcgggc actttttttg
    39421 gggactacaa actaactttt tggcaggatc aagcatacga ggcatcagct tatcaaaatg
    39481 ttagtggcat aagcaaagga acttacacat tgagtgccta tgtaatgtct gggggagagt
    39541 ttgatactaa tcagttatat atcaaaaatt atggtggagc ggaactaaat actgcggttg
    39601 ttaaaacaag tgaatggaca aaaattacaa tcaaagacat tccaattaca acaggtgttt
    39661 gtgaaattgg ggtttatagc aaggcttcag gaaatgcttg gacgaacgtt gattttgttt
    39721 cattggtgaa acaatagact gcggatcgtt tcgattcaag atcggaggtt cccttcagcc
    39781 acatcgttag cgtagaaggc aggaatcgaa cctgcacctc gcctttgcca aaaataacca
    39841 gaaacgattt ccagtcattt ttaccaaaac gagtaaagca ccagacttca tctacacttt
    39901 gtattatgct aaagagagtt caacgatttc gtaagaagca atgaaaaaat tgcaaaaagt
    39961 tgtgcagata acataaagtt tgtttaagaa agggtaacct tagaattccg tcgtgaatat
    40021 gatttgagca cttagatgga tagaaaaaag ttctcggaaa acagttcgtt tttcgagaac
    40081 ttctttttat gataagaatg aaacaaatta acttatatta tagtctcttt tggtagggaa
    40141 ccatcttatt tttaatgaaa atcaagcatt ttagctattg aagaatgttg atttataggg
    40201 attccccctc catattatgt tggctcttta ttcccactcc actgttgctg gtggtttaga
    40261 agtgatgtca tacacgatac ggttgacgtg ggctacttcg tttacgatac gtacagagat
    40321 tttttgcaag acttcccatg ggatacgggc aaagtcagcg gtcattccgt cgattgaagt
    40381 tacagcacgg ataccgactg tgtagtcgta agtacgtccg tcacccataa caccgacaga
    40441 gcggatccct ggaagaacag tgaaatattg ccagatatca cggtcaagtc cagcagctgc
    40501 gatttcttca cgcaagatag catcagattc gcgtacgatt tctagcttgt cttcagtgat
    40561 ttcgccaaga acacgaatac caagaccagg accagggaat ggctgacgcc aaacgattga
    40621 atcaggcatg cctaactgag tccctaattc acggacttcg tctttgaata atgtgtttaa
    40681 tggttcgatc aattcaaatt gcatatcttc tggcagtcca cctacattat ggtgagattt
    40741 gatcgtctgt gctgtttctg taccagattc gataacgtca gtatacaacg ttccttgtgc
    40801 taggaaagag acgccttctt caccagcaag ttttgttgct tcgtcatcaa aaacatatac
    40861 gaattcattc ccaatgattt tacgtttttg ttctgggtca gatacacctt tcaacttgtc
    40921 taagaagcga tctcttgcat tgactttgat gatgttcaag ccgaattttc cgcctagact
    40981 ttccattact tgttccgctt cgcctttgcg tagcagaccg tggtcaacaa agatagatgt
    41041 caattggtca ccgatcgctt tttgtaaaag aaccccaacg acactagaat caacgccacc
    41101 tgaaagacca agcaagactt ttttgtcacc aacttgttca cggatttttt ctacttgcat
    41161 atcgataaag ttatccattg tccagttacc ttcacagtga caaacatcaa aggcaaagtg
    41221 gcgcagcaat tcgattccgt attcggaatg acgtacttct gcatggaatt ggataccata
    41281 aaaattacgt gttgtgtctt ggatagaagc aatcggacaa tctttgcttg ttccgactgt
    41341 ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg ctcatccata ctgtttgttg
    41401 ttttggtgtt tctttaaaca agacagcttg atcgcttgtt acttctaaca aggctttccc
    41461 atattcgcgg tttttagcag gctctacgga accgccaagt ttgtacgtga tcaattgcat
    41521 accatagcaa atccctaata caggaatacc taaattgaag atctcaggat cgatcccaaa
    41581 tgcattctca tcatatacac tgtttggtcc gccagaaaga atgattccct ttggattcat
    41641 cgcttttact tcttctgcag taatgcggtg gctcaataat tcggaaaaga ctccaagatc
    41701 acgaatacgt cttgtgatca attggttata ctgactaccg tagtctaata cgataatctt
    41761 ttcaacgttc gtcatatcgg caacgtttgt caaatttatt acccctatcc ctttaaattt
    41821 tatctcaaaa tagtcaaact attttaaaat gctaatattt tcgcatgaaa atcgaatcga
    41881 tgatatgatt ttcagtttta tggagaatta tatcggctct tcctcgtgta ggcaagatat
    41941 attcctgtaa atttttcaaa ttcacatttt tccatacatc ttttgccatt ttaaaggctt
    42001 cttcacgatc tccgatagca aaacgataat aataattctc tggattttgg aaagccgtat
    42061 ccagtaacgc accaaaacga tccaaatacc atttttcgat cagcacggga tcggcatcga
    42121 catagacaga aaaatcaaaa aagtcgctga cgtagatttg ctgattcgca ggtaattgaa
    42181 gcgtattgat cccctcaacg atcaagatat caggctgctg gatcaattcg tacttgtctg
    42241 gaatgatatc atacacatca tgtgagtaaa caggtacttt tatctcttcc tgaccagatt
    42301 ttacacggtt caagaaatcg atcagcatct ccatatcata actttcagga aatcctttac
    42361 ggtccatgat tccacgctct tttaatatac gatttggata aagaaatccg tcagtcgtga
    42421 tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat cgtctgcagc agtctggctg
    42481 ttgtactttt tccaacggct acacttccgg cgatcccgat aataaaaggc ggtacttcta
    42541 cgtatttatg aagaaataaa cctttactca agaacaaaga ttcaaattct ttcatataaa
    42601 gataaatcag atgagcaaga ggtacataga tatctcgaac atcctgcatg gaaatgcggt
    42661 cattcaaact tttgatatta tctagttcat cttgtgtcaa aggtgcttgc ccattacgat
    42721 aaaattctcg ccattcttct cttgccacac ggtagtagtt cattggatca ttcatattag
    42781 cctcctgcgt tctcccatca atggaactat tttaacacaa aataaacgca aaccccacca
    42841 cttaagagag attgccgatc tttttgtata atctttataa tagaaagaaa cggagtctta
    42901 ttcttgactt gcgcacggat acataaaagg atagactgtt gtcatgaaag aaatgagaag
    42961 gatggaagac atgacaaatc attactattc tgaacgccca gatacggcgc acgattttga
    43021 acaatgggct tttgagttaa aaggcagaac ctttcaattt gtaacagatt ctggtgtatt
    43081 ttcacgtgaa acagtggatt atggttcacg tgtcctgatt gatgctttcg attgggaaga
    43141 gttacctgat gaaggaaaga tcttagatgt gggatgcggg tatggaccga ttgggttagc
    43201 tttggcattt gcgacacagc ggtttgttga aatggtcgac attaatgaac gagcggtaag
    43261 tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat gttgatatcc atcaatcgaa
    43321 tatctatgaa gcagttcatg aagaaaccta tgcagctatc gtaagcaatc cgccgatccg
    43381 tgctggaaaa aaagttgtac atgaaatttt gacaggcgca tatcctcggt tgaagaaggg
    43441 cggtacactg acaattgtga tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat
    43501 ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat aaaggatatt atatcatcaa
    43561 aagtgtgaaa gaataactag ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt
    43621 gcatgtacgg gtacgtatca gttctttttt tgttgagaag tttttaaaaa caaagaacta
    43681 ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt tttttacgat aaaataaata
    43741 agatacagaa cttgtataga aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa
    43801 aacatacttt ccttttttct ctattctttt cttgggggtt ttggtacact gatagggaat
    43861 ttagatagaa gggagccaat aggatgaagc tgaccatcag agatatcgca gaaatggcag
    43921 gcgtatcagt aacaactgtc tcacagattt tgaataacaa aggaagcaga tttagtgata
    43981 agacaagaaa aaaagtgttg tcgatcgtga atgaacatca ttataagccc gattatttcg
    44041 cttcaaatgt gatcaatcga cattcaaaga ccatcggaat gatcgtccct gatgtcactg
    44101 atttcttctt ctccaaactg atcgaaggtg tagagagcta tttgaatcct ttgggctatg
    44161 tgatcatgtt atgcaattct cgtcatagcc aagaaaatga gatcaagtat ttacaagagc
    44221 tttcccaccg ctcagtggac ggaatcttgc tggccacacc aaatatttta cctgagaaat
    44281 atgcattaga cagcggtttt tatcaaaaaa tgccaatcat tttaatcgat cgcggattga
    44341 atcgtcggga taacggacgg cttatcgtaa aggaatatga gggcgcctat caagctgttt
    44401 cgctgtttat tgaaaatggc catacgaaga tcggcatgct gaaggaaacc actggatact
    44461 atcaattaga agaacgtttc aacggatatc gtcatgcgct gaaagatcat ggcctgcctt
    44521 ttaatgggaa atttgttgaa catggagagt tgactgtcca aggtggatat actgcatcaa
    44581 aaaaattact caaacataaa gatatcacgg ctattttttg cgggaatgat gccatggcga
    44641 tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat cccagaagat atctcagtca
    44701 taggattcga tggattaaag ctgtcagaat acatgattcc gcaattgacg acagtgcagc
    44761 agccaagttt tgatattgga ttttatgctg cacgtttcct gatcgatacg atcgaatttc
    44821 ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa
    44881 aagtattgac aaagggctaa ctcggcggta tattgcttat gtaagcattt acaaaggcta
    44941 gttagtgctt tttttatttt tagtaaacca gtttactaaa gtggtttacc tttcctaaaa
    45001 ataatgtata ataccgaatg aagacaagga ggatcaggca atgcgaatgg tggatctgat
    45061 tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa
    45121 ctatacgaat ggaaagattc cagattatca agtcagtgca ttattgatgg caatttttta
    45181 tcaagacatg acaaatgaag aaatcacgaa cttaacattg gcgattgcta attctggtga
    45241 tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat aagcattcga caggtggcgt
    45301 tggtgacacg accacattga ttttagcccc cctagtagct agtgtcggag tgacagtggc
    45361 taaaatgtct ggacgcggtc taggttacac aggtggtaca ctagacaagc tagaagcaat
    45421 tcccggattt cagatcgagc tttcggatga agaatttgta cgcattgtta atgaaagtaa
    45481 agtagcggta attggtcaat caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt
    45541 gcgtgacgtc acagcgaccg ttgattctct tccgctgatt gccagttcga ttatgagtaa
    45601 aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg accactggag acggtgcttt
    45661 catgaagaac atcgaagatg caagacgtct ggcaaagacc atgacgagca ttgggaaact
    45721 ggctaaccga gaaacagtgg cggttatctc agatatgtca gaacctcttg gcgaagctat
    45781 cggaaatagt ctagaagttg tcgaggcaat cgaaacatta caaggaaatg gtccagaaga
    45841 tctagtagaa atgtgctacg ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa
    45901 gacaaccgat gaagcaagaa cgttgcttca agaagctctg gaatcaggta aagcgttagc
    45961 aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca gcgatcgtgg aacatccaga
    46021 acgaattttg actgcacgct acacaatgga gcttcctgct aaacaaagcg gagttgtgtc
    46081 aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac
    46141 caaagaagaa gatattgatc acgctgttgg attgaaactg cataaaaaaa ttggtgacac
    46201 tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac gacgaagaaa tcacttctgt
    46261 cattgaatta ttatataaaa atattgaaat tggtgaatcg gcaatgaagc caacgttgat
    46321 tcatgacatc atcacagaat aaaggaaaaa agcggacttt accgttttaa agaaaagggg
    46381 aaaagaaatg gaactaaacc gaatgatcga ccatactatt ttgaaagcag acgcatctaa
    46441 agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac catttttatt ctgtctgcat
    46501 caatccagca tgggttgcgc tagcgaaaga acaattacaa ggtgagccag tagccgtatg
    46561 tacagtaatc gggtttcctc taggagcaaa cacacccgag acaaaagcat ttgaaacaac
    46621 agatgccatc aataacggtg cagatgaagt cgatatggtc atcaatatcg gtgctttgaa
    46681 atcaaaggat gatcaacaag tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga
    46741 tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta gatagagaag aaatcatccg
    46801 agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc
    46861 aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt gaaactgtag gaccagaaat
    46921 gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc
    46981 tggtgctaca agaatcggaa caagcgcagg agtagcaatc gtatctggtt caactggtga
    47041 aggctattaa ttttttcaaa ggagaagctc atggcagaac tcaaacaaga atggatcaac
    47101 acagctattg aagcgttaga taaagcctat gttccttatt cccattttcc ggtaggcgct
    47161 tgtctcgtaa cggaatcagg caagatttat caaggcatca acattgagaa tgcatcattt
    47221 ggattaacga attgtgccga gcgcacagct tttttcaaag cagtatctga aggagaaaaa
    47281 acatttacgc atctagtcgt agcggggcac acccccgatc ctatctctcc ttgtggtgca
    47341 tgtcgacaag taatggcaga attctgttcc cctgatatgc cggttacgct agtaggagac
    47401 aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa
    47461 gatctataac tcatgttcat tcagtcagct gactgttgaa aaataaaaca attattttag
    47521 gaggctttac agaaatgaaa aaagcaaaat tatttggttt aggtgctgtc gcactagcag
    47581 cagggttatt tttaggtgca tgcggcaaca acggctctac tgattcatcc ggtggcaaaa
    47641 gctcatcaga cacaacaact gcagcattga tcacagacac aggtggtgta gacgaccgtt
    47701 cattcaacca gtcagcttgg gaaggactag aaaaatgggg gaaagatcaa ggactttcac
    47761 gaggaaatga tggcttccaa tatttccaat cttcaaatga atcagattac atcccaaata
    47821 ttgaccaagc attgaatgct ggattcaaaa cgatttttgg tattggttat aaactaaaac
    47881 cagcgatcca agaacaagca acaaacaata caggtacgaa ctttgttatt atcgatgatg
    47941 tcatcgatgg attagataac gttgtatctg caactttcaa agacaacgaa gcttcttatc
    48001 ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt agtcggcttt atcggcggtg
    48061 taaaaggtga agtaatcgat cgttttgatg ctggattcaa agccggtgtt gatgcaggtg
    48121 cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata tgctggtgac ttctctgctc
    48181 cagataaagg acgttcaatt gctcaaggta tgtatgcaca aaacgcagat atcatcttcc
    48241 atgcttcagg cggtacaggt aacggtgtat tccaagaagc aaaatcattg aacgaatcag
    48301 gcgacaaaaa agtttgggtt atcggtgttg accgcgacca atcagacgaa ggcgaataca
    48361 cattaaacgg cgaaaagaaa aacttcacat tgacatctac actgaaagca gttggaacag
    48421 tagtagaaga tttggcacaa aaatcagcgg acggtaaatt ccctggcggc gaacacactg
    48481 tttatggatt gaaagaagac ggtgttggtc taacagaagg acaactttct gacgaagcga
    48541 aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa
    48601 ctcctgaaga aaactaaaac catttctata gccttgttag acacatgttt gacaaggcta
    48661 tggtttgatt agaacaagaa ttgttcattt atccaagctg ccttgtgaca gtttggataa
    48721 gggaacaact ccctgaagta atgtttgttt caaaagaaga acgctcacta agcgtgaatc
    48781 ttagtcaatc gttgccctag atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa
    48841 aatattgtca tagaaatgca gaatattacc aaagaatttg gaacattcaa ggcaaatgac
    48901 aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt tgcttggaga aaacggtgct
    48961 ggcaaatcta cattgatgaa cgtgctttcc ggattattag agccgacatc agggaaaatt
    49021 ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca aagccaacca attagggatt
    49081 gggatggtcc atcagcactt tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg
    49141 ttgggaagcg agcctagtcg cgcagggatg cttgaccata aaaaagcacg aaaagagatc
    49201 caaaaagttt ctgaacagta tggattgtca gtcaacccag atgcttacgt tcgtgatatt
    49261 tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac tttaccgagg agcagatgta
    49321 ctgatttttg atgagccgac agctgtattg acccctcaag aaattgatga attaatcgtg
    49381 atcatgaagg aattagtcaa agaaggcaag tcgatcattt tgattacgca taagttagat
    49441 gaaatcaaag cagtagctga ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca
    49501 gtcaacgtta aagacgtaac ctcacaacaa ttagccgata tgatggttgg aagaacggtt
    49561 tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag tcgttttgtc tattgaaaat
    49621 ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa acttgaactt agaggttcgt
    49681 gctggcgaag tacttggtat cgctggaatc gatggaaacg ggcagtcgga gttgatccaa
    49741 gctttgactg gtttgcgaaa ggcagaaagc ggacatatca agctaaaagg ggaagacatc
    49801 actaataaaa aaccccgaaa aatcactgaa catggtgtag gacacgtgcc agaagaccgt
    49861 cataaatacg ggttagtcct agatatgaca ttatctgaaa atatcgcact acaaacctat
    49921 catcaaaaac cttacagtaa aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc
    49981 agagaattga tcgaagaata tgatgttcga acaacgaatg aacttgttcc cgcaaaagct
    50041 ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat
    50101 ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag gtgcgatcga atttattcat
    50161 aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt tactgatcag tttcgaatta
    50221 gaagaaattt taaatgtttc ggatcgtatt gctgttatcc atgaaggaga aattgtcggt
    50281 atcgttgatc cgaaagaaac atctgaaaat gaattaggtc tattgatggc gggctatacc
    50341 ttagaagaag cacgaaaaga gttagaaaag ggggctggcg cacatgaatg atcgcagcga
    50401 aaaattccgt aatttattag tgcctgtact ttcagttgtt ttaggattga tcttaggcgc
    50461 gatattgatg gctgcttttg gttttcagcc catccaaggt tattcttcta tgctagatgc
    50521 agcccttggt tcgcaaagag ggataggtga aacgttaagg caagcaacgc cgttgatttt
    50581 tacagcatta ggtttttctg tagctaactc agctgggttt ttcaatattg ggctttctgg
    50641 acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc
    50701 aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc
    50761 ggctgttcca ggtttacttc gagcattttt tggaacaagt gaagtgatcg taacaatcat
    50821 gatgaactat atcttattat acgtaagcac atttgcttta catgaagtga tgcctgaaag
    50881 ctatcgctca agtttggact cttccaatat gatcagtcaa aatgcctcac tgcggttacc
    50941 atggttaaca caagcttttg gcggctctcg cgtaaactca ggattatttt tagcaatcat
    51001 cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact ttaggatttg aaatccgttc
    51061 tgttggattg aatccttttg cttctgagta tgcaggaatg agtagtaaac gtacgatcgt
    51121 tttgtcgatg gtcatttctg gcgctttggc agggcttggc ggtgtggttg aaggattagg
    51181 aacgtatcaa aacttctttg ttcaaacgac ttcgctatct attggatttg acggaatggc
    51241 tgtttccttg cttggttctg gatcagcaat cggtattctt ttatcagcga tcttgttcag
    51301 tattttgaag atcggtggac ttggcatgca gacaggagca ggtgttccgt ttgagatcgt
    51361 caatgtatcg attgctttga ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt
    51421 catggctaaa gtcttccaag ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa
    51481 accaacgaat caaaaccatg aagggggcga actataatgt cgactatcga attaattgcc
    51541 ttgatcctta cttctacact ggtttattca acaccgctaa ttctaacgtc actaggcgga
    51601 actttttctg aacgaagcgg gatcgtaaac gtagggctag agggaatcat ggtcatgggt
    51661 gcttttagtt ccatcgtttt caacttgacg atgagtccat cacttggttc tatgacacca
    51721 tggatcgcta ttttagtggg tggcgtcgta ggtatcctat tttctctgat tcacgcagta
    51781 gcaacagtca gtttgcgtgc tgaccatatt atcagcggga cagtaattaa tctgatggca
    51841 cctgctttag gtgttttctt gatcaaagca tggtatggta aaggtcaaac tgataacatc
    51901 acgcagaatc tggggtattt ttctttccca ggattagcag atattccggt cattggacca
    51961 attttcttta aaaatacatt cttacctgct tattgtgcta ttttgattgc tgtcctagct
    52021 tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct
    52081 caagctgccg acacattagg tatcaatgta tatgctatgc gttattcagg cgtactgatt
    52141 tctggcttgt taggcggtat gggcggtgct gtttttgctc agtcgatttc cggtaacttt
    52201 tcagctggta cgatcgttgg acaagggttc atctccatgg ctgctatgat ctttggtaag
    52261 tggaatcctt taggggcaat gggtgcctca ttgttctttg gttttgctca aagcttgagt
    52321 atcattggcg cacaattacc agttatttct tcgattccgc cagtattgct gcaaattgct
    52381 ccatatttgc tgacaatcat tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa
    52441 gcaaacggga agaactatat caaatcaaaa taatctttta gcgaataaaa agggtgggtt
    52501 acgggaagtg tttttcgtct cgcccttttt gttttaaagg caataaaaat aaaaagaaag
    52561 aaggaataat catgtttaaa cgcgtacatc tgatcgtcat ggattcagta ggaattggag
    52621 aagcaccaga tgccgataag ttcggtgatg ttggttctga tacattagga cacattgcga
    52681 aagaagcagg attgaacgtt ccacatttag agcagcttgg tttaggaacg atccgtcctc
    52741 ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc gacgaaatta gaagaagttt
    52801 ctgtagggaa agatacgatg acaggccact gggaaatcat gggattgaat atcaaaactc
    52861 catttcgtgt gtttcctaat ggattccctg atgatctgat caaacaaatc gaagaatttt
    52921 ctggccgtaa agtcgtatgt aacaaacctt atagcggaac agctgttatt gacgattacg
    52981 gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac ttctgccgat cctgtccttc
    53041 agatcgcggc acatgaagaa atcattcctt tagaagaatt atatcggatt tgccaattcg
    53101 ttcgtgatat aacaaaagat gatccgtata tgattggccg aatcattgca cgtccgtacg
    53161 tgggtgaacc aggagacttc acacgaacaa gcaatcgcca tgattatgcg ttgaatccat
    53221 tcggacacac agtccttgat tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa
    53281 agatcaacga tattttcaac ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata
    53341 tggatggcgt tgaccagctg ctgaaagtta tgaaaaacga tttttcagga atcagcttta
    53401 ccaatttagt agattttgat gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc
    53461 atgcgattga agaatttgac ttacgaattc cagaattgct tgaagcgatg gaagaagacg
    53521 acttgctaat gattacagcc gatcacggaa acgatccgac gtttactggt accgatcata
    53581 cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc
    53641 ctcaaggata ttattcagat atttcagcaa cgattgctga gaactttagt gtttcttcga
    53701 ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc tttttaaata ggagaaagca
    53761 aatgaccaag ttaagcgaga tgttacaaga aacgacccag tttatccttg ataaaggagt
    53821 aaaagaaatt gattttggtt tgattctagg ttcaggatta ggtgaattag cagatgaaat
    53881 cgaagatgct gtaatgattt cttatgatga gatcccgttc tttcctgttt cgacagtagt
    53941 tgggcatgca ggaaagctag tttatgggtc tctggcaggc aagaaagtgt tagccatgca
    54001 agggagattc cactactatg aaggtcattc catgcaaaca gtgacctatc cagtacgtgt
    54061 gatggccgga ctaggggccc attcagtcat tgtgactaat gcatgtggtg gtgtcaacga
    54121 atcatttgta ccaggagatt tgatgctgat taccgatcat atcaatttta ctggtgaaaa
    54181 tcctttgatc ggtccgaatg aagatgcaat gggccctaga tttccagata tgagtcaagc
    54241 ttatacgcct gactatcaag caacagcaaa agcagcagca gaaaaactag gtttggatct
    54301 taaagaaggc gtatatatgg gctattctgg accaacttat gaaacgcctg cggagatccg
    54361 aatgtctcgg acaataggag ccgatgcagt agggatgtcg actgttccag aagtcatcgt
    54421 agcagctcac agcggattaa aagtgttagg aatctcttgt attaccaact tagcagcagg
    54481 tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca actcagagag tcaaacaatc
    54541 ttttaaagca ttggttaaag aaacattgac attattgtaa taggaggaac aataaaatga
    54601 gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa gattttactt ccgggagatc
    54661 ctttgcgagc taaatatatt gcggagactt ttttggaaga tccagtttgt tacaatcagg
    54721 tacgcggtat gttaggcttt accggcaaat ataaaggaga acgtatttct gttcaaggaa
    54781 caggaatggg aatgccatca gccaccatct atgcccatga attgatccag tcgtatggtg
    54841 taaagaaatt gattcgagta ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg
    54901 atttagtact agcacaaggt gcagcgacca gttcttcgat gatcgaaaaa aatttccaag
    54961 cctttcattt tcctccaatc agtgatttca atctattact gaaagcatat gagatagcta
    55021 aagaaaaggg ctatacggtt catgtaggaa atgttctttc ggaagattct ttctacaaag
    55081 atgatttgac agagacgttc caattagctg aacttggcgt tttaggagtg gaaatggaag
    55141 cagcggctct ttattactta ggcgcgaaat accatgtgca gacattaagt ttgatgaccg
    55201 ttagcgacca tttgatcacc ggtgaagaaa caacagctgc agaacgtcaa tctacgttca
    55261 atgaaatgat cgaagtagga ttggaaacag cgatagcaga ataagaagga aatgaaaaag
    55321 ctgccctgca ttaagtaata agaacatcca agcaaaaata gctcctcatg tttttgactt
    55381 ggctggactt attattgcaa atgtaagctt ttgaacaccg tttaccaaga ggttgtgaa
//
LOCUS       DASJST010000004        42173 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJST010000004 DASJST010000000
VERSION     DASJST010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.004"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..748)
                     /locus_tag="U4B05_000167"
     CDS             complement(38..748)
                     /locus_tag="U4B05_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049335.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(766..1263)
                     /locus_tag="U4B05_000168"
     CDS             complement(766..1263)
                     /locus_tag="U4B05_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049336.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(1300..1752)
                     /locus_tag="U4B05_000169"
     CDS             complement(1300..1752)
                     /locus_tag="U4B05_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2049337.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(1887..2492)
                     /locus_tag="U4B05_000170"
     CDS             complement(1887..2492)
                     /locus_tag="U4B05_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2049338.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(2942..3562)
                     /locus_tag="U4B05_000171"
     CDS             complement(2942..3562)
                     /locus_tag="U4B05_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2049339.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(3564..4433)
                     /locus_tag="U4B05_000172"
     CDS             complement(3564..4433)
                     /locus_tag="U4B05_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN2049340.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(4456..4863)
                     /locus_tag="U4B05_000173"
     CDS             complement(4456..4863)
                     /locus_tag="U4B05_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2049341.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(4882..6171)
                     /locus_tag="U4B05_000174"
     CDS             complement(4882..6171)
                     /locus_tag="U4B05_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2049342.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6198..6500)
                     /locus_tag="U4B05_000175"
     CDS             complement(6198..6500)
                     /locus_tag="U4B05_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2049343.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(6525..8618)
                     /locus_tag="U4B05_000176"
     CDS             complement(6525..8618)
                     /locus_tag="U4B05_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2049344.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9107..9871)
                     /locus_tag="U4B05_000177"
     CDS             complement(9107..9871)
                     /locus_tag="U4B05_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN2049345.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(9982..10851)
                     /gene="mdcB"
                     /locus_tag="U4B05_000178"
     CDS             complement(9982..10851)
                     /gene="mdcB"
                     /locus_tag="U4B05_000178"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN2049346.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(10876..11952)
                     /locus_tag="U4B05_000179"
     CDS             complement(10876..11952)
                     /locus_tag="U4B05_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN2049347.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(11976..12569)
                     /locus_tag="U4B05_000180"
     CDS             complement(11976..12569)
                     /locus_tag="U4B05_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN2049348.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(12573..14249)
                     /gene="mdcD"
                     /locus_tag="U4B05_000181"
     CDS             complement(12573..14249)
                     /gene="mdcD"
                     /locus_tag="U4B05_000181"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN2049349.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(14239..14538)
                     /locus_tag="U4B05_000182"
     CDS             complement(14239..14538)
                     /locus_tag="U4B05_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN2049350.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(14640..16286)
                     /gene="mdcA"
                     /locus_tag="U4B05_000183"
     CDS             complement(14640..16286)
                     /gene="mdcA"
                     /locus_tag="U4B05_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN2049351.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(16293..17204)
                     /locus_tag="U4B05_000184"
     CDS             complement(16293..17204)
                     /locus_tag="U4B05_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2049352.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            17324..18181
                     /locus_tag="U4B05_000185"
     CDS             17324..18181
                     /locus_tag="U4B05_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2049353.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(18368..18730)
                     /locus_tag="U4B05_000186"
     CDS             complement(18368..18730)
                     /locus_tag="U4B05_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN2049354.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            19076..20515
                     /locus_tag="U4B05_000187"
     CDS             19076..20515
                     /locus_tag="U4B05_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2049355.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(20522..23074)
                     /locus_tag="U4B05_000188"
     CDS             complement(20522..23074)
                     /locus_tag="U4B05_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN2049356.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(23188..24520)
                     /locus_tag="U4B05_000189"
                     /pseudo
     CDS             complement(23188..24520)
                     /locus_tag="U4B05_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(24486..25964)
                     /locus_tag="U4B05_000190"
     CDS             complement(24486..25964)
                     /locus_tag="U4B05_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2049357.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(26269..28104)
                     /gene="tdc"
                     /locus_tag="U4B05_000191"
     CDS             complement(26269..28104)
                     /gene="tdc"
                     /locus_tag="U4B05_000191"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2049358.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            28574..30001
                     /locus_tag="U4B05_000192"
     CDS             28574..30001
                     /locus_tag="U4B05_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2049359.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(30093..30458)
                     /locus_tag="U4B05_000193"
     CDS             complement(30093..30458)
                     /locus_tag="U4B05_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2049360.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(30455..30820)
                     /locus_tag="U4B05_000194"
     CDS             complement(30455..30820)
                     /locus_tag="U4B05_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2049361.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(31012..31260)
                     /locus_tag="U4B05_000195"
     CDS             complement(31012..31260)
                     /locus_tag="U4B05_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049362.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            31259..31387
                     /locus_tag="U4B05_000196"
     CDS             31259..31387
                     /locus_tag="U4B05_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN2049363.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            31444..33138
                     /locus_tag="U4B05_000197"
     CDS             31444..33138
                     /locus_tag="U4B05_000197"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN2049364.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            33142..33261
                     /locus_tag="U4B05_000198"
                     /pseudo
     CDS             33142..33261
                     /locus_tag="U4B05_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            33912..35045
                     /locus_tag="U4B05_000199"
     CDS             33912..35045
                     /locus_tag="U4B05_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN2049365.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            35076..35396
                     /locus_tag="U4B05_000200"
     CDS             35076..35396
                     /locus_tag="U4B05_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN2049366.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(35425..35889)
                     /locus_tag="U4B05_000201"
     CDS             complement(35425..35889)
                     /locus_tag="U4B05_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049367.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(36020..37273)
                     /locus_tag="U4B05_000202"
     CDS             complement(36020..37273)
                     /locus_tag="U4B05_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2049368.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(37302..37619)
                     /locus_tag="U4B05_000203"
     CDS             complement(37302..37619)
                     /locus_tag="U4B05_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2049369.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(37645..37965)
                     /locus_tag="U4B05_000204"
     CDS             complement(37645..37965)
                     /locus_tag="U4B05_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2049370.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(38429..39844)
                     /locus_tag="U4B05_000205"
     CDS             complement(38429..39844)
                     /locus_tag="U4B05_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2049371.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(39861..41177)
                     /locus_tag="U4B05_000206"
     CDS             complement(39861..41177)
                     /locus_tag="U4B05_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2049372.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(41327..42025)
                     /locus_tag="U4B05_000207"
     CDS             complement(41327..42025)
                     /locus_tag="U4B05_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN2049373.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT        12547 a         8834 c         7212 g        13580 t
ORIGIN      
        1 aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct tctgattcat
       61 ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt ttttccttca
      121 acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat tcaatgacca
      181 gcaccgctgc ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc cagttgagac
      241 agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt gccggagcat
      301 cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct aaatgcagat
      361 ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac aaggaaatac
      421 tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga tttgcacgtt
      481 tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct
      541 gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg taatttcttg
      601 cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct tttgttacgt
      661 ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat acttcccaga
      721 aattggtatc cactattact tttttcatct gttcacacct cttgattaga ataattgctt
      781 gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt cgctgtattt
      841 gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag
      901 ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta gtaaaaatgt
      961 accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt ctaaaaaatg
     1021 atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc
     1081 ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt ctgtaagttt
     1141 ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat tatcttttcc
     1201 aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt
     1261 catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc
     1321 ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta aaagatattc
     1381 caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt
     1441 ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat
     1501 ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat
     1561 tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat
     1621 tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc
     1681 tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga tgtctcgaag
     1741 gatttcagcc atttcatccc acctttattt tgtttgtaag actagttcac ctctttttgt
     1801 tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact agtaaagtat
     1861 attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa
     1921 ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta
     1981 aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc
     2041 gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc
     2101 gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact
     2161 tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac
     2221 cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca
     2281 cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct
     2341 tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca
     2401 aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt
     2461 agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta
     2521 tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc
     2581 tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg
     2641 ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac caaacgaaaa
     2701 atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac
     2761 aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat
     2821 caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc
     2881 tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt
     2941 gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt
     3001 gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc
     3061 ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat
     3121 cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt
     3181 cacgatcacg tttgtaccga cgttgccata acacttcaaa gtctgctgtt aggcgaatcg
     3241 tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac
     3301 taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt
     3361 tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa
     3421 acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg
     3481 gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca
     3541 gaagaatgaa atactttttc atctcattta tttcctaagt taattggact ttccaagttc
     3601 ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca
     3661 ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc
     3721 cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca
     3781 ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac atcaactact
     3841 gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga
     3901 ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg
     3961 tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg
     4021 ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact
     4081 gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa
     4141 gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc
     4201 aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc
     4261 atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct
     4321 tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact
     4381 gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat
     4441 tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat
     4501 ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc
     4561 tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt
     4621 attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa
     4681 tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat
     4741 ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc
     4801 tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa
     4861 catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata
     4921 taagagtgct gcggctactg tatctacatc ggttgctgta gcattgttga atcccatcga
     4981 attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc
     5041 gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc
     5101 ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt
     5161 aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg
     5221 gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat
     5281 agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa
     5341 tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg
     5401 atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag
     5461 cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg
     5521 caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac
     5581 accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc
     5641 agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc
     5701 gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac
     5761 tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt
     5821 tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc
     5881 tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat
     5941 cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat
     6001 gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat
     6061 gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt
     6121 gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca tgtttttacc
     6181 ctcttttctt tttgtattta gataactccc ttatcttgca gaactggggt gattttttct
     6241 tcaatttcat tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact
     6301 gcgaactttt cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg
     6361 gaaactgcgg aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa
     6421 acatcctcag cagacatctg tgctgcaaaa ctacttccta aacctgcacc gcatacgaat
     6481 aaaatgttca atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact
     6541 gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc
     6601 tcttcgttgt tgatcaattc aattagttct ttcatgatgt tcagatgcga gtaggagtct
     6661 actgctgcca aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt
     6721 ggttgctcaa tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca
     6781 tgggctagtg ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga
     6841 atcatcgctt cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct
     6901 agttcaatcg attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc
     6961 aaaatatctt ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg
     7021 aataattttt ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg
     7081 acatctatta tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt
     7141 agttcttcat ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc
     7201 ggatcgacaa ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga
     7261 ttgacatcca atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg
     7321 atctgaggaa acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg
     7381 ttacagacaa cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc
     7441 gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact
     7501 tcttcgatcg taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg
     7561 atgttttctt tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca
     7621 gccatgtgct tatagagatt ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt
     7681 gtctcgttct ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg
     7741 attgaattgg agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt
     7801 tctacttgcg ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa
     7861 aagctactgt cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc
     7921 ctgattcgct ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct
     7981 tcttgtttca acaacttatt ggaatagatc tcctgtattt ttttgatttc caccaaagga
     8041 aaataatgga gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag
     8101 tcggtcattc ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct
     8161 gaacctttat aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc
     8221 agtgttgctc gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct
     8281 tcttcttttt tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca
     8341 aaaatacgtt cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat
     8401 cgagcttgct tttccttttc cagcttcaac aaaaaacctt tgttgcgaat tgtacggatc
     8461 tccggaagct ttttactgct caaccagata ttcgcttcaa gaacttcctg acggatcgtc
     8521 cgctgactga cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc
     8581 atgattctgt gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt
     8641 caagtatatg gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa
     8701 gaaagtaagc aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa
     8761 gacatacgcc tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa
     8821 gctgtcctgc atcaagtaat aagaacaact aaactaaaat agtgcttcct atttttcgtt
     8881 tggttgttct tattatcgca gatgcaagct tttaaacacc atttagtgga gaggttgtga
     8941 cagagatgtt cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta
     9001 caaaatcgtg ccttattgcc gataggctat ctctggcaca ccgtttattc agaaaaagga
     9061 gctgcgacaa ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa
     9121 tcggctgaat aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata
     9181 tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat
     9241 cgactgaata atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct
     9301 gtcgataatt tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg
     9361 ctttgttttt ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc
     9421 tggctttttc aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga
     9481 tttccagaaa ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg
     9541 ctttcagcct tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta
     9601 ctgacaaaac agctaatgca agaaatcccg tttgctcata actagaagga cctgtacctg
     9661 gaccataata cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat
     9721 atgaacgacc accaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat
     9781 gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca
     9841 aaatgattgc taaataattg atttttttca ttgtcttcct cgttatcttt tattacaaaa
     9901 aactattttt atgatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt
     9961 tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacaaatgt atgtgtcggc
    10021 agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc
    10081 tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg
    10141 taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc
    10201 actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat
    10261 ttgtttctgt acatatgaat cattatctag ctccttagct gcttgaattg caagcctgat
    10321 atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac
    10381 cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt
    10441 gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc
    10501 aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt
    10561 tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa
    10621 ctcctgatca atagcatgat gccatgctat ttgagccatt tcttcaaaaa aaggagttag
    10681 cgtcttagca gacttaagaa ataaagcata gtccatatcg gaatgtgcac ccgcatccag
    10741 tgcatccact aatccaggtt ttggacttaa tgttacttct gcgatcaatg cttcctctac
    10801 caacgcagcg agttgctgag tcactgcttt ttttcgttcg tcatttatca tgacactcac
    10861 tccttcttcc tactattact tgctgaaacc ttttttgata gctacccaaa tgatatgaag
    10921 taaccagctt aaagcaaatg cacaacttgc cgtcattaga taagctacaa gaatcatgac
    10981 tcctgaaggt aactgcagcc aactatcttt aaacaataat aacagtactt ggaaaacaaa
    11041 tacattggct aaatacgtac gataagcata aacagacaac catttgaaca atggcaattt
    11101 tggcgaatcg tttacaatca ttttggaagc gcctatgaag acagcaaaga tgatgactaa
    11161 actgtataaa ctttggatcg tattcaagta aggtgcattc gcaaagctca agtctcctgg
    11221 atgtgcgagc aatgaacgga cagaaaagaa cgcgataact aaggcaacag gtaagaaaag
    11281 ataacgtact ctgtccaaat attgatagac tttttcgtga taaacaaatg cggctacccc
    11341 ataaatcatg aaactagcaa caaaccgatc taataagtac cagctgtcat gataaggccc
    11401 agtcaatact tgtgtttgat agaacagata ccatgcgaca tataaaatcg ttgaaccaat
    11461 caatactggc catacatatt ttttattagc aaatactttg taaccgagcc aaacaaaaaa
    11521 cggcatgaac aattggatct gcagcatcat aacggtatac cataaatgag gcgctccatc
    11581 acctgtgaca tatttcagta aaaagcttgc agtatccgta tacggagttt gttgttggac
    11641 tgctggaaat agccaaagat aagccgtcgt ccatagaaga tacggaacga ctagttcaaa
    11701 aaacttttct ttcaaaaatt cccaataaga caaatgctga cttgttttta ccatattata
    11761 aacaattgca aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa aggtaccaag
    11821 aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca aagctcaata tcgtttgcat
    11881 cataacagcc gttacagctg atactttctg gaagtcactt acgttgattt tgattttttt
    11941 gttttcaaac atctctttct ctctcttttt ctttattacc aaagttcttt ggttaattta
    12001 ggtccttcga ctgttttcaa tagaattttt ttctctggct ctctcaaata ctcttttagt
    12061 gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa agcaggatgc
    12121 gattgaatta cctgaattgg caattcaatc ggtgaatttg cgtacagcag caaatcaaaa
    12181 tcactggttt cttttactgc cggaatccca gtcgataatt caaatcctaa gctgccgccg
    12241 attccccatt tacatcctga taatatgttt ctagcagcat gaaattgctg ataaacagga
    12301 taatccatga ttttctcttg tctatacgat tcttgttcga tgatgtccca cggatgaatg
    12361 acttgtttga cggcatgttt tgagatttct gttgaccatc tctgattgcg ggcaggtcca
    12421 cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac tgttccgaac
    12481 atcttttcag ttagaacgac ccattcaggc aaggtgaacc cttctatttc atgtttatca
    12541 aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat cccattcttc gtccattttg
    12601 gcacggactt tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt tgtgtatcga
    12661 tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt gattgcttcg
    12721 atgactttgc tgacattttg ttctgaagca gcttggtcgg tgatatcttc taggaaatga
    12781 tagatcgctc ctagttttgt ataattattg atatcgtaag cgatagctgg aactttttca
    12841 gcagctgctt cgatttcagc aaccgaacgt tttgtcacac gtgcagcact ttctttagac
    12901 atcgcttgga cggtaatttc tttattgttt aaagcaatca agcggcttga ttgtaatcca
    12961 tgtgctaaaa atgcacctga aatagcattt cctgcaatca atccaataac tggatgtcca
    13021 gcttgacgga gttttgcata agcagcagca ctattagcca aagccacatg gataccgatc
    13081 aattcttcgt tatagccgta tgcttggctt ggtacatcga tcacaacgat gattggccgt
    13141 ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc catgcggaaa
    13201 ccttcaacta aaccaacttc accgtttcgt acacggtaca tcgggttttt aggatcaggt
    13261 acgattgatg tcacgatggc ttcttctatg aatttacttg atttaccata atacacagta
    13321 ctaatagaag aagttggatt tttcatgcca gttagtgccg caaaccaatc gtaaccaaca
    13381 cttgctgcag ctggttcatt tccttctgct acttctggta agtcaattga ttttgcttga
    13441 tggttagcaa ttgcttcatt gtaatcgtca atggccattg gttttgaaag atctaaagaa
    13501 tcaagtaatg ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt tcgttcgtcc
    13561 attgctgcga tgacagcagc tttgattgct tctacactat cagtcactaa ttcatcaatg
    13621 atttttgatt ctacccgttg acgagatcct aatgtattcc aaatcaagtc tttgtcacta
    13681 gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt caaaccgact
    13741 ctcgcttttt ttgttgcgat taaataactc attaatgctg aagtaatcga cataccgcca
    13801 aatgatccta cacgtcctgg aatcaaaccg atgactggta catattgttt tagagcaatg
    13861 attacatttc cgatttctga aatagataac agtccgtagt ttgcttcttg caaacgaaca
    13921 ccacctgtat ctagtacaat gactggaaag atctcatttc cttttttatt ttcttctaac
    13981 acatgttcaa gtgcagcaac gatttttgca ccggaaactt caccgatacc gccaccttgg
    14041 aattttcctt ccatagcgat tactactgct tgtttgccat tgatcgtgcc acggctgaca
    14101 acgatcccgt catcactttc agggacgatt ccttgtgctg gtaaacttgg cgcgatgatt
    14161 tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac tcgttctctg
    14221 gcgtttaatt ctacaaaact atttttcatc tgaaagttcc tccattgctt gcgtcaatcg
    14281 taggtacaca actcctggtg tagcaccgaa gtcattgatc acgatatctg ctgaaattgg
    14341 ataacgatca aaaaatctcg ttaatacatt gttccatact tcgccgaagc catcgcttcc
    14401 tgtacaaatt tttacagtag attctgtttt ttctgttgga aacatcaaga tttccaaatc
    14461 tcctgaaccg accactccta catggatttt cttattgatc ggctgtttcg ttggatattt
    14521 ataagctaat tgttccatgg atctctcccc tttttgtaaa acagagcata cacgttctgc
    14581 ttattcgtct ttcttcttcc atctgggtaa atggcgacaa aaggtttttc tcgccaattt
    14641 tatgaccaag ttctgaattt cattggtggt tcatacaggc catctgacca ttcgatcaaa
    14701 tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt cactacgttt gatgcctaga
    14761 tcttctggta atttcacgat gccggctgca cgcaagctat ctagtgattc tttcgtgcta
    14821 gtcattccaa gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg ttcttctttt
    14881 gtttttgctt tgtacaaata agcaattcct tcttctgtca cgatatgtgt agtatcttcg
    14941 ctataaatca tcactggtgc attggctaag cctgcttgtt tttgaacagc tagtgcatct
    15001 aattcttcga caaagactgg ttttttattt gaaccatatg tttcaaccat ttgaacaact
    15061 aatttttggc ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca tgctggtgta
    15121 gaatgacgac gaccgccagg attatgtccc atgttaggcg caccgccaaa accagataaa
    15181 cgtccttttg ttacggtaga agagtttcct tcgtaatcta attgcaaggt tgaaccgata
    15241 aacatatcga tggcatattg tcctgcagct tgtgataatg ttcggttact gcgcatcgtg
    15301 ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa tatatttttc cataccaact
    15361 tcgccaccaa aactatggat gctttcaacc caacctgatt cgatggctgg aatcatcgtt
    15421 ggatgtgggt tcaataccca gttcttcgcg attttccctt ttaaccctag tgattcacca
    15481 taagttggca gtaacaattc gatagctgct gtattgaatc caataccgtg attcaaagat
    15541 tgtacgccgt gttctgcgta gattcctttg attgccatca ttgccattag aatttgcaat
    15601 tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta attgataagg tttatctgct
    15661 ttcacgacaa cgtctaccca gtcaccaggg atatctacac gtggtaaaga atcgacaact
    15721 tcgttaactt gaacgatcac gataccatct ttgaaagctg cagcttcgat gattgttggt
    15781 gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt atctgcagct
    15841 actaaaacaa catttggaat caaatcgatg aataagcgtc cataaagttc caaataagta
    15901 tggatatcac ctaatttcat tgtgccgtct tcaatcattt gggcgacacg caagctttgt
    15961 gcaccagcat aagagaaatc gatcttgctt gcgattcctt tttcaaaaat attcaaatgt
    16021 tctggtcgtg agatactaga catgatcaaa tgcagatcat tcactttttc tgcatctact
    16081 ttttcaagac tttctgaaag aaaacttgct tgtttttgat tgtcgccttc taatacaact
    16141 ttgtcgccgg gagcaatcag tttttccatt acttctacga ttttttcagt ttctacatac
    16201 ttgccattga gcaagttgct gattttttcc aatcgctgcg cttttttttc gcgtttagaa
    16261 gcccagttgc ggtttggttt ttccataatt agtcatctcc atttttgata tagaaacgtg
    16321 gcgtcatcaa taccatgttg gtctagattg atcgtacgga tccctttatc tacaccaaat
    16381 tttgtatgaa tcagctttgt caaagtattg ccggggggaa attcaatcgt cagatccatt
    16441 cctaattctt ttgccaagtc catcatttgg ttccactgca cagggaaagc taggttttcc
    16501 aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt attttttata
    16561 tacaaacatc tagcatcaga tagatggaca gactgcatat acggcttgaa tttcttgacc
    16621 gtatcattca tcaagagaca gtgagatggc acagggactt tcagcagctt cgctgtttgc
    16681 gcttggtgcc gtttggcttc tgccaacgta acggataaac cttcgatact cccagataac
    16741 gtatgttgca tcggacaatt ttcatttgac agataaactg gatggtcagg atcaaatgtt
    16801 tttgctacaa tttcctcagc ttcgcttctc gtcacaccta ctactacgcc cattgcataa
    16861 ccgcttggat aagcttcttt cattaagact gcgcggtgat agactagttc cgctgcattc
    16921 tcaaatctca aagatcgaca agcaacagct gcacaaaaag caccaatcga atgtcctgct
    16981 gcaatatcag gctgaatacc gtttttttcc atttcatcta aataaaatac tgcttttgtc
    17041 agtaatgcta gctggataaa aaccgtatct tgatagtttt cttctttatc aactaaccga
    17101 taccccgtca cattttctaa acgttccatg tacatttcag ggatatccgc taacatccct
    17161 aacttctgac tgccttgtcc aggaaataag aaacctgtac tcatgagcct cgcctcctca
    17221 gcacttactt tttccactgc tatcttagtc aatttctgct ataataaaaa atgccaaaaa
    17281 aagaaaaaac cataacaaaa aggtatggat gggatggata attatgaaat tgcttcatct
    17341 gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca aagaattatt
    17401 tatttctcaa cctgccttga gccgtgcgat caaacatttg gaaaacgaat taggtgtccc
    17461 tttgttttac catagcggca gaaacattga gacgaccgtt tatgcagaag aattttatcc
    17521 atatgcagtc aagacattag acacattaga agaaggaatc aaattattgt cttcggtcaa
    17581 tgagaagatc gttacttccg tcatattgta tttagaggta gcctctgttt ctattccgaa
    17641 cttggtccga atttttatgg aaaagcatcc tgatatccaa ttaacgatca tgcaacatca
    17701 tttaccagat gatattaccg atcctgtact gtacatcact tcggagatga agcctggcat
    17761 gaccaatatt cctatcatga aagaacctgt ttatgtcgcc attcctaaga aacatcctct
    17821 tgctaaaaaa gaacatctgc aattagaaga tatccaacgc acccccatct tgatgctgtc
    17881 aaagagcaac gccttccgtc atacactgga tgcagcactt gaagcgaaaa gtatcgaatt
    17941 gacgatcagt tcgacgacag atgatcctgc gacattacgt tctattgtca agcaaggatt
    18001 agctgttagc tttttcccaa aagtttcttg gtcttacgat aaaaatgatc ctttcgtctt
    18061 gcgggaaatc aaagatttgc ccttgacacg aacaatttat ttatcttcta gcttcacaga
    18121 agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt taggaaaata
    18181 aaaaaaaaag agctgggaca aaattcttgt cgcagctctt ttttcgaaca aacggtgttc
    18241 cagaagtaat ttcttagaaa ataagccgaa atttcccaaa aatttgagaa gcaattttcg
    18301 taaattcctt cttatttctt gaagctaaac atttctgtca caacctcttc tctattgtcc
    18361 tagacattca ttgcgttgat ttaaaacttc catcaatcaa atctgctagt tcatctagct
    18421 tttctatttt acgtagatca atagagatcc aatgttcttt attcatgtga taggctggaa
    18481 aatacccctc ttttcctcgc aaaattccgc caagttcagg atcgaccttc aaatcaagaa
    18541 tattgatgat cctatggtca cttgcatcat catagatttt ttcttctgga acagacatca
    18601 ggaccccaaa ccattttttg cttttttgat gtctgaatac gcaataatca ggaaattttt
    18661 taaaaatata ttctggagac aaattgtatg tttcattgat gtgatcgatt agttcttttt
    18721 tcgttagcat ctgcttccat ctctttctta ttgattcgct ttattcaaag ataccacgta
    18781 agaatagaaa aatgaataca agtagttgct aatcaaatac ttcgatattt ttctttttta
    18841 tcaactctta aatgttcaat ccgcctgtgt tggtcttgct accatctact attaaaatgt
    18901 ttattattgt ttgcccacct attttttgat tttagtggta tcatttttca agttcatgat
    18961 gaaattctta tcatcagtaa atgctccaga aaaataaaca atgattgaaa aatcagttga
    19021 aacttgacca ccgtttattt tctccgtatt cttagaatag aagggagtct gattgatgta
    19081 ttcactaatg aaaaaaatca ttaccgaaaa agatattcga cgtcatgttt ctttagtaga
    19141 acaattgctg aatcatacaa agataaccgt aaatgaatta gctgaaatca tagggacaac
    19201 cgaacgaacg attttttctg accttcaatc tattcgctct cagctgcccg aaggctggga
    19261 catcatcagc gatcaagcag gaatcagttt gcaaaaccag caaaatttat taacaaatga
    19321 cctttgggag atttttttca aacaatcagt cagtgtcgaa ctgctgaaaa atctgctttt
    19381 tacaaaaaaa gttgctgtcc ctgattttct agccgaccat gggctttctt atgggacatt
    19441 gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt tacgacttgc agatcgattt
    19501 gacgaaatat acagcttgta ttcttggaaa agagcgtgcg attcgtactt tttatcatcg
    19561 attgctgatt cctttcactc ataacaacta ttttttcgag gattattcga tccatgaatc
    19621 ccattatttt caatttttaa gaaacctcag tcaaactgaa ctagcggttg aaacagaaga
    19681 gattttcgga acatgttggt tctttatcaa tacgatccgt ataaaagcga attgccgatt
    19741 agattcttct atccatacca acagcacact ctcctccctg tatgactctg cattgaaaaa
    19801 attgtattta acggaaggta tttatcttaa agacactgag ctttcttttg cttctttctg
    19861 ctttctggaa agttggaatt acaacaataa ctatggacaa gaaaccgctc gctgcctgca
    19921 tcattctcca tttttagagg tcctcgaaac gtttgtagag gaactagctt cagagctgtc
    19981 tcttgatcaa ctaaaaaaaa catctctgac tgataacttg gctctcctca tattaaaata
    20041 tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa taccatcgtt ttttagaaac
    20101 atatgatcag caatatgcac acttgtatca acagaaagag aatttactga acgatctaaa
    20161 aaaagaaata gaaatcaatg aaccaaatta ctttctccaa ctcttgtctc tactgatcca
    20221 acaaacgatt ttttctatca aaccgaattt gttcaatgtc tattttattt tccaaggaga
    20281 accttcatgg aaggcgttct tgcagcagga attaaaagat tacttaggaa aacgtgtctg
    20341 cttcttacct atcgaactaa ccgatctttc cactatttct tttgaaaaaa cagatatctt
    20401 gatttccaat ttccctctag attctctaga tatccctatt gtatacattt cttctattcc
    20461 aacgaaaaat gagctcaatc gtttgactga actcactttc aaaacttttt tatagtaacg
    20521 gctagcgact gtattctcta gctgtttttt cttttttgcg aaacagtttg ttcatgatcc
    20581 ctttttctat ttctacaact aagaatagaa gaatcacatt tgctccaatc aataactgtt
    20641 gtgtcatcgt taaacttact gtaccgatca gctgctggcc gactggtaga aaaataacca
    20701 tcccttgcaa taaagcaagg atacctaatg aaagaaaaag cgctttgttt tgcaaaagac
    20761 cttggttcaa tgacggcttg gacaactctc tgcaattgat catgtaaaca gcttgagcta
    20821 agacgatatt ttgcagaaga atggtttgct gtaagctttg cccttcaaac tgcatagcca
    20881 ataaataggc aggaaccatg atcagtaggg agacatagat gatccgaaac aaatgataac
    20941 cagataggat tccttgcttt ggatctcttg gtgggcgctt catgatttct ggatctggcg
    21001 actcgaaccc tagtgcataa gataaagtga ttgtcgttac catattgacc cataaaatct
    21061 gtactgggga caaaggtagc gggcgattca gcataagtgc ccaaatcaag atcaatcctt
    21121 gagctaatgc agttggcaag aagaaattga tggttttttg caaattatcc aaaatccgcc
    21181 ggccctcttt tacggctttt gcaatcgtat ggaaattatc atctcctaaa accatatcag
    21241 ctgcctgttt gcttacttca ctacctttga ttcccatagc aattcctaca tcggatcttt
    21301 tcaaagcagg tgcatcattt acgccgtctc cggtcattcc gacgatttct ccatttttct
    21361 gcaaagcttt aacaattcgc agtttatgtt ctggtgtcgt tcgagcaaag acatcgacta
    21421 ttggcacttg ctgaatcaac tcttcatcag acataagatc caaatcgatc ccttctaaaa
    21481 ctttggcagt atgttttaag ccaatttgtt ccgcaatcgc ttttgctgtg tctttgtgat
    21541 cccctgtaat cattttcact gagatccctg cttgttggca ttcctttaca gctttgactg
    21601 cactttcttt tggcggatcg atcaatccgg cgatccctgc aaaagttaat tgggttaaag
    21661 tctcatgcgt gagttcctgt ttcgaatcta cttttttata agcaaatcct aaaacacgct
    21721 gaccttttct cgcaaattct gcagctgtct gactccatgc ttcttgttct gcagaagaaa
    21781 gagttgattt agcaaataat acttctggtg cacctttgac gtaaatcacc gctccttttt
    21841 cctccgcatg catcgttgcc atatatttgt attcagaact gaacggaatc tttgcttcta
    21901 tttttttcaa aggtagattg gctgtttctg tgtgtcgaat cagcgcaact tctgtcggat
    21961 tgccacttaa attttctgtt ttctgttctt cttttgtttt taattcctga cagtttttca
    22021 taatggacaa cacgcaggct tcttcttttg cagctacatc cacaactgtc atttcatttt
    22081 ttgtcaatgt tcctgtctta tctgaacaga tcactgtcat agaacctaat gtttctaccg
    22141 aaggaagtcc tttgatgatc gccttttctt ttgccatttc atgtactcca acggatagaa
    22201 tcatggtcaa aacagctggc aatccttcgg gtaccattga caccaccaaa gcaatcgtag
    22261 ccgaaagaag aatatgccaa tccataccat atcgaaatgt cgtaaaaata actaagaata
    22321 ggctcaaaca aagaatgcct tgaaaaattt gtttgtttaa tcggttgatt tttttaatca
    22381 gaggcgtcgt ttgttgttcg accgattgca atgcttgatt gattttaccg atttctgtcg
    22441 tgtttcctgt ttctacaacg acaccgactg cagaaccaga ttgtacaagc gttcctgaaa
    22501 aacccatatt tttctggtcg ccgatcgatt cttcttctgt caaaacggca tgactttttt
    22561 cagatgcttc tgattctccc gtcaaaatcg attcttccac taataaatca tgtgcttcga
    22621 ttaatcggat atctgctgga acgacatctc cggcacgtaa gacaatgaca tctcctttta
    22681 ccagttcttt cgcagggatt ttttcttgca tcccgtttga tagtactatc gcttcttgtc
    22741 ccatcagctg cttcaaacca ttcaatgatt cttccgcttt tctttcttgc cagtaactaa
    22801 cgagtccatt gacgatcact acgaataaaa taatacttcc ttcaatatat tcactagaag
    22861 caaatttcaa aaaagaggca atgatcaaca cgatcatcag cagatcagta aaatgctttg
    22921 ctattttttt ccatcgtttt gtctgctgcc ttgcttttat ttcattatat ccttgatcag
    22981 tcaggcgttt ctgtcgttct tcttgcgaca agccttctgt tgatgtttga tagtttttca
    23041 atacatcttc taccgtcaat ctgtaccagt tcatcctatc ccctcctgtt taattgatac
    23101 tgttattata cgcagggaag ttttttcagg cgtatcgatt atttcagggg ggtctgaaat
    23161 aattcgtttt gcaaaatgta tctgggccta actgatattt ctattgctta ttcttttata
    23221 tacaaaattt aagaatacaa accagataac caataaaaaa ggaataaagc gtgtcgtgct
    23281 ttcgaataac aagctgaaag caattgaagt aaaaaacacc aacaaagcaa gatctttata
    23341 tctttgtgta gtgttccgtt tcttttcttt aataaattga acatgtgcac acaagatcga
    23401 agaccacaca aatagaaaac aactcgttgt catgcctgcc aaaagctgaa aaaacaatcg
    23461 actatcagct acaaaaaaat gtagacaaag accggcaaaa aaataacatt cctgtaatta
    23521 aaataccgtg taaaggtacg cctttcttag aaagcgcaca aaaccaatgg ggcaaacttt
    23581 tttcttttgc tgtttgatat aaaatcctgc tagtactgta gactgcacta tttgctgagg
    23641 acaaagaagc aaccaaaatc attagattga caattgctgc tccgccttgg caatggatca
    23701 cttgcagcat agtcactaaa ggactttctt ttaaatctaa acaatgccat ggaacaacaa
    23761 ataaaatcga caaaatcgtt cctatataga aaaagagaat acgccaaggc agacagttga
    23821 ttgctttttt caaattcttt tgcggatcac ttgtctcgcc aacggttaag ccgatcattt
    23881 caattccagc aaaactgaat aaaaccatag gaaatgagac taccattcca gcaaagccaa
    23941 acggtagtaa ttcatcgatc tgtccaaaat tttctttgat tccgatagac tgggtatgaa
    24001 aatctgagac actcaatcct atacccaata agataaaaag gaaaatcccc aagatcttga
    24061 tgattgagaa aaaagattcc aattcaccaa accacttgac tgcaacaagg ttgactgcca
    24121 ttaagatcat tagtgcgcta cctccgctta tcaaagctgg tacatgtaca aaccagaaat
    24181 ggatatacaa tcctattgct gttaattccg caagtccagc tccaagccag caaagccaat
    24241 aagtccaatt gacaaaaaat gcccactttt tgcctaaata atagtcaacg aaatcactga
    24301 tagagttaaa tgtactattt gctaaaaata aatcacccat ggcccgcatc aaaccataaa
    24361 tagccaaacc gcatatcaaa taaacgagaa taacagctgg ccctgctgat gcaattgcct
    24421 ttgctgaacc taaaaataat cctgttccaa ttgcgccacc taatgcgatt aactggatat
    24481 ttcgattagt tagagtccgt ttcattgccg gtttttccat caagttcacc tttttctttt
    24541 tcaagcagca tggcgacttc ctgttcccaa gaaggtttct ttattttttc aatacagagt
    24601 ggtactacaa acaaaatgac actgatggct gtgactgcaa ttagattgat ccatgttcca
    24661 gctttcatcg tcagtccgac agaaaatccg attgccgcaa ataaaacaat aacgacagtc
    24721 caaactccaa gattgctctt tttctctttt tttccaattc taaatggtcg attcaacttt
    24781 gcatgtgtat aacgcaatct caaaactgcg atccccatga tgacatagac gatacagtag
    24841 ataacagtag ttgaatttac gagcatcaaa aatgcttgat tgacgccagg aatcagcaaa
    24901 taaaaaagtg caaagattga gataatgaca gactgagtca aaataacgga tttggatacg
    24961 tcaaattgat tcgtcttcca aaaattgaat tttggtggat acgcaccttt tctagcactt
    25021 tctgtcactg tctttgctgg tcctgaggcc caagcagata attggactgc tacaccaatg
    25081 aagacaagta aactaaacag attcacaata agatgtggca aaccaagaat ttggcaataa
    25141 atcgaaatag attgcgcgat attattcaat tccatttgtc cttttggtac gacatttgct
    25201 acaactaaag cattcaaaat attgacgaaa aatataaaaa ttaacgcggc aaatactccg
    25261 cgaatatacg ttttaactgg attttccaat cgagtgatat acacagagga catttcgata
    25321 ccagtaaaaa taaacatgat cggcgcaaaa taaacaaatg aggatgcagt cgtagtatct
    25381 ggtattaatt tgtcccatga aaaactgcct aatgttccgg ttggtacgat cccaactttg
    25441 atccacgcag caaaccctaa taagagcatc atcgtcaaag gcacgtacaa tcctaaccaa
    25501 acaccgactt ttccgccgat ttttgccata tcaaatttca aattcaaaaa agtgatgatc
    25561 caataaacga ctaaaatgac aactaatgta aatttgctgt tcaaaccaag cggcacatta
    25621 tcgataatat taccaaacaa cggagcgagt gctgaagcga ccatcaccat cccaggaaac
    25681 atttggaccc ataacagcca tgagacaacg aacccccatt tttttcctag agcattcgat
    25741 acccaaagtt ctggaccacc tttttgggga aacattccag caaattcacc agagatcaat
    25801 gagattggaa aagcgtacaa taatactgca accagcatgt aaaaaatcat cgtccagctc
    25861 gttgctgcta catctggaat atttcgtaca cttgctacaa gggcgcaggt cattccgata
    25921 aaggtcatgg tcgtgatttg ctttgatgaa ccagtatctt tcatctttat tccttctttc
    25981 aatagcgaca tatgtttttg ttttctttta tttttgttca agaacagaaa tgacaaggtt
    26041 tcatcgattc agtacaaaac ccagtatcac cgaaacatcc attgaacgat cttagtgaac
    26101 tgggtttgat acatgaaacg aacgatccaa cttatagaac gttttataaa aaagaatatc
    26161 ttggcgagga atatatgaaa aacaggggga aatacaattg ttttgatgaa gcaaataatt
    26221 gtctgttctc catatgtctc ttgttattct ttcacacaaa aatcagttct attggatgat
    26281 ttcgtttagt ttcttttcca ttgctttggc gatttttttt gtataaaaat tgaaaattcg
    26341 atcatcattc aaataaggag tcatgatagc cgcacgtagc agagtgattt tctgttcttt
    26401 tttccattct tctttcgtta gacccattct ttcaacaaaa cgaatcggag aatcaccgta
    26461 atcttcttgt gtaaatgtag tatgagaagt aataaaccgt tcaccataaa catcgccatc
    26521 catataagaa gaccgatcga acattttttc atttaattca ttgatagcgt tcaaatctgt
    26581 acagccttgt tctttgaata cccaattgac catattaaaa tcaggatgat tcaatggata
    26641 gacttcgatt gtttttcctt taacagtaaa agtcagatga tcgagaaatt ctctgaatct
    26701 ttgagctgcc tcgattgaag ccccaatcaa ttgaccatat ccggtcacat tcaatggcaa
    26761 tacacgatgt gctgtccaga ctgcggcggc ggttgctcca gcttttgatc cttccaaaat
    26821 ataagctcct aacatatctg gagcctttac tgatttttca aacacatatg gcgcgaaata
    26881 agaaatgata tttctcatat ttttatgttt gattacgatt cctccagcag cataagggac
    26941 atagcccatc ttatgaggat ctatcgtgac agaatccgct tctgaaatag ctctgaaccc
    27001 ttcatatacc tctttgtcaa tcgttacgca gtggtggaaa acatgatgtt cttcaaagaa
    27061 ttcagctaag gaagcatacg gcacaaattc tcctgcttcg tttagaaaca gtgaacgcgc
    27121 atagccgcca tatgcggcat caacatgtaa atagaaatag atcccttcat cttttaacct
    27181 ttctcttaat tggacgattt tatctacact gtctacttga ccttcttctg tcgttcctac
    27241 gacagcaacc acacctagaa ttgggatttt ttgtccagct aattcacgaa ttgttttttc
    27301 caaagcatta atatccattc gataatcctc ttgaactggg atagcaacca tctgatctaa
    27361 gccgactccg caaatatcta acgccttcat ccaagaatag tgttttgttt gagggaccag
    27421 ccatttccct aatctttgga tattttttcc gctacgggaa gaagctgctt tgacctcatc
    27481 catttcttcg tccgtaaagc tttcggtcat ttccaaaatt tcttcaacag ataaattcaa
    27541 aagttcccac tcagacattt ttttcacttt ttcaggcagc acttccttga cagctaaagg
    27601 aatcgatttg atgcaacggg cgtaccataa tccttcaaga ttagcaatag aaccatctgc
    27661 tgcaatgtgt ccccatccgt ctgtcatatt gaataatgaa gcaaaatcct gtcctacttc
    27721 tgcttccatt tgcgaagttg ccatagaaga ttccaatgca acattatttg ggttccacag
    27781 catcgcatag ttgtaagcta ataaagcagg catcaatgtt tcagcattca tttgtcccca
    27841 gtaacggcct gctgaatgcc atgggatcga tcctgcgcgt aatcgctgac tgacttcatc
    27901 caagacctct atcatatggt cgcgggtggc tagataatct ggagtgagct tatctccttc
    27961 gctaattgca ggcatatctg aaggaatata attttctctc catccaagat gttcatctac
    28021 catcttgttc aacaacattt tataaactgg tccattttca gctttgtcgc ctataaagac
    28081 ggccttgata tccatatctt tcatttttct tccctccata ttttgttggt caatgtcttg
    28141 cagatacatg tgtttatctg taacacctct tcactatagc gccaaaaatg agaatttttt
    28201 ttttataaca tcaaatccac gtagattttt acaaacaaaa ataaataaag tcggattttt
    28261 ctgataattc aacgcaaaaa agcgaacaat ttttcaataa atcagtcttc taaaatagat
    28321 aacctaaaaa aagcgaacaa acaaataaaa tgactgtcct tcattcacta aacatatttt
    28381 tattttttta tattgttaaa aattcgcaat ttatttttta atagaattat ctgttttcca
    28441 aattttataa cataaaaaac cttttttaac ggcatttata tgctttttca gaaattaact
    28501 gatgatttca aaaagaaaac atgtcataat atttcccata ttgtgcaaaa aaatacaagg
    28561 cggaggctct actatgattg aagaatatat tgagaaaaat attttaagac agctgttttt
    28621 atgtgggcaa ttctatgtaa ataaagaagt caatctagaa aagctctcca atctcctgca
    28681 tgtttgcaaa accactttac tcaatgatat caataacata aaaaaagaat ttgaagaaca
    28741 gatcgcttat acacatcggg aaaaggaccg ttacacttta tatttttcag aacatatacc
    28801 tcgatgcaag atcatgcagc aattgtctca aaattcgttg tttttaaaaa cttgtctgct
    28861 ttatttggaa gaagatgaac ctgattatct tcaattaact gaatgtgaat tcatctctgt
    28921 cagcaaagct tattcattaa aaaaacaagt attagcttat tttaatgact gcgggatcga
    28981 aatcgaccgc tactcccctc gattcactga aatggaaaga cgtctgctgc ttcttaatgt
    29041 gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct gaatcgtttt ttgaacgtgc
    29101 cgatcgtttc attgaatccg taacggaaaa tagtggcaga ttttatgata aagaaaataa
    29161 agaaatctta tcgattggtt ttgctatcag tttcctacgc cagcaagtgt gtgctgtgac
    29221 aatagattca aaatttatcg aagaaatcaa aaaacgtcct atttacaatt atgtcgaatc
    29281 agcatgggaa aacactgatt tccaaaccta ttataaaaaa gaagaattcg cctttatttt
    29341 gacactattc aatctttgta attatggttt ccattcttat cagttgattg cggaagactt
    29401 tcaacagcta caccaagtgt ttattgacaa tactcccgaa ataaaagaac tagttgctac
    29461 ctttgaatct cacttcaatc aagaattatt cggaaaccaa ccgttcgaac gtgcgctgat
    29521 tcatctgatg cgttcggctt gggataacta tcaattattc atgccagaaa aattttatct
    29581 tttaaatgag gaacagacaa atttatataa agaagtccag acaatttttt ccagctggtc
    29641 cagccagtta ccttatgatc tacggttgaa tcctaattgc atgcgggctt ttgtgatcga
    29701 attgagcgga attctccgtc tgacaaaaga acatctgact atctatatcg tcacaaattc
    29761 ggatgttcat tatctgattt atcgggaagc cttggaagct gtaactactt tcgattttca
    29821 agttgcacca acaatttatt caagtatatc tgatataaag aagtatgcgc agcagtcctc
    29881 aaaccgggta ttatgtgaac gtacattata tacaccagat gcggtccaat atgaaaatat
    29941 tattcctatt tcgatcaata cgatcgatcg agctatcatt tcagctgtgc aaaacaaata
    30001 aggggctgtg acaaaaatca tgtcacagcc tcttatttgt tgaagatgcc catttctgta
    30061 acaatctctt gatacacgtc aggctcacaa cctcacttat tttggacatc tacgggtccg
    30121 atctctttat ttaaaaaata agaaatgatg gttcggataa ctaccaacgc cgctagttgg
    30181 aaaatatcct gcaaggtagg tttgataatc gattcgatga tcccagctga aacaagaatt
    30241 tccaaactta gaagtatata gcctcccaac atctttttaa tagcattgtt ctcgctgact
    30301 cggtcgcttt gcacttttgt atggacaaat aaatatttca aaaaatcctt gattgataga
    30361 caaacacccc aaaccaataa acagatagca aagatatcaa gtcccagtac aagtttttcg
    30421 aaatatggaa gaagaaactg catcaactct gtcgtcatcc tacctctcct cttctttgtt
    30481 tttctttgtc ttctatttcc tgattcaaga aataagaaat gactgttcga atgataataa
    30541 taagtgcaag cttcaaaata tctgtccaag tcggcttgat aactgactcg atgatatcag
    30601 caacgactag tacttccaag cttaacaaaa tataacttgc tagcagtttt cttgtatctg
    30661 cattacgagt attacgttct gcatagtttt tcttgacatg agaccaatca aaaagtttga
    30721 atagaacaag aacgatcccg acaattaaga taccgataga acaaatattt aaaatagcaa
    30781 tgatccattc tagaaattca attaaatttg agcgaaacat atacgctacc tactttcttt
    30841 taatttgttc ctattctttt attgtaaaag aaagttacct tgttcgtcta tttaaaagtt
    30901 gttctaatga taacggttcg cttaaatttt tctttttcac tctcccattt ttctccctcc
    30961 atccataaat cgtggaagta tccaaaacaa cgtagcaagt taaaataagg cttagtccgt
    31021 catcagcttt tcaaagaagc actgtctcgg tgcttttttt gtacccaaat ttctaaaaat
    31081 agaaccatac acctcctcaa atcttttctt cttacctacc ctatttttat ttacaggaaa
    31141 atcagacact tcttcttttt tgtcaaaatt tcctttttct tctcgtccta tcatttggca
    31201 ttctaataaa aacgaactat ccttggatat agaaagagaa aacaaggagg cgtttttcat
    31261 gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg cttgcccttg gcgctgcata
    31321 tctaggaaaa aagacaggac tattcgaaga tgacagtcat ttatatgatg aattcgaatc
    31381 aatctagcat cctatttagt tgaatcacta cggacatact tggaagacat tggaggaatc
    31441 acaatgagaa aaagtaaagc aataatgatt ggtgccggta ttgccaatat ggcagcggct
    31501 gtttatttga ttcaagaagg caaatggaaa ggggatcaga tcacctttta ttcattggat
    31561 gatcacggtt caaatgatgg tgcaccgacc gacactgtag tggatgaata ttggaataaa
    31621 aaccatccat tagaaaacca aaaaggctat attgctcgag gcggtcgcat gcttaattac
    31681 cgtacctatg tagatttgat ggatctgtta agtcgaatac cttctgttac tgagccaggg
    31741 atgactgctg aagaagatac gagagatttt gatgcaaaac accgaacctt tgacaaagca
    31801 cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa aactgggatt caataataaa
    31861 gaccgtattc ttctgactaa actgattgct atgcctgatt cagaagaaga aaaactagac
    31921 aatatcacta tcgaagaata tttcaaagat gatccacact tttttgaaac aaacttctgg
    31981 tttatgtggg aaacaacatt tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc
    32041 tatatgcatc aaatgattta tgaattcact caaatcgaac atctagtggg cgtcaatcga
    32101 acacgttaca atcaatacga aagtattatg cttcctttga tcgagtactt gaaagaacaa
    32161 ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg aattcaaaga aacaccgatg
    32221 caagatgaaa tcactgtgac tggtttgaaa attaccaata cactgactca agaagaagaa
    32281 tacgttgtgg tcgatgaaga tacagcagtt attttcacta atgggtccat tacagattca
    32341 gctactttag gagattacga aacaccagct gtagaaaata tggattatgg tgcagcttct
    32401 agtctctgga aaaaagccag tgaacgtttc tacaacttgg gaaatcctga caaattcttt
    32461 gctgatcgag atgcaagcga atgggtcagc tttaccctga cgacgaaaaa ccatttgttg
    32521 ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa atgccttgaa ttcatttatt
    32581 tcaaccacac cgatcacgcc gttaggccaa aaagatgtca atatgtctat cgtcgtgcat
    32641 catcaaccgc attttacgtc tcagaaacca aatgaaacag ttatttgggg ttatttcctt
    32701 tatccaagac gacgtggcga atttgtggat aaacagtata ttaaaatgaa tggaaaagaa
    32761 atgctagaag aactgattgg acaactttca aaagtagatc ctggaccagt gaatattcgt
    32821 gaaaaagaaa cagaaatttt cgacagcgta ataaataata tccctgtcta tatgccttat
    32881 gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac caaaagtcat tcctaaacat
    32941 tcgaccaatc tggcctttac aggagaattt gtcgagcagc cttatcagat gatctttact
    33001 gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc attttgcggg tattcctatg
    33061 tcccgtcttg tcaaaacacc gcgttacgac aaagatattc ctactctagc tcgtgccgca
    33121 aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg ccctgcatca agtaataaga
    33181 acaaccaggc taaaacagct tcttatgttt ttgatttggt tggacttatt accgcagatg
    33241 caagcttttg aacaccgttt accaagaggt tgtgaaaaag ctgccctgca tcaagtaata
    33301 agaacaccca agctaaaact gcttcttatg tttttgactt ggttggactt attaccgcag
    33361 atgcaagctt ttgaacacca tttaccaaga ggttatgaaa aagctgccct gcatcaagta
    33421 ataagagcag ccaagctaaa acagcttctt atgtttttga cttggttaga cttattaccg
    33481 cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaaggcgt ttagtttcga
    33541 gtatgaagaa aaattttgtg aaaatggttc ctcacgtttt tgcataaatt tgacttaata
    33601 ccgagaaact gatcccttct gtcataacct ctttttattt atgtctgttt ctttttgaaa
    33661 acaactattt tttcaacgtt tactctcttg ccaatccaac atcatctaac ggatacaact
    33721 atttcaatga ttaccattac actaaaaaat cggaaacaca cctctccgaa cacaaataca
    33781 taagactata gaatgtttca tttaagaaaa aaagcgtttc attatcaaaa aggtaagatc
    33841 tcctttaaaa tgaaattatc tgtagtacta ataatgcaga caattttatg aatctgaaag
    33901 gagcatctcc aatgaaagca gtcacttggc aaggaaaaca gaaaatggaa atcagaaacg
    33961 tagatgatcc aaaaatatta gaaccaacag atgcaatcat ccgtatcaca gcaacagcca
    34021 tttgcggctc cgatctgcat ctttaccatc atggtgaagc agttttggaa aaagggtatg
    34081 ttgtaggaca cgaaccaatg ggaatcgttg aagaagtggg atctcaagtt caaacattga
    34141 aaaaagggca acgtgtggtt atccctttta atattagttg tggtcattgt catttctgcc
    34201 tgaatcacat ggaaagccaa tgtgatgaat caaatccaga tcagttatat ggcgggcttt
    34261 tcggatttgg caagttgaac ggtaatcatt ggggtggtca agcggaatat ctaagagttc
    34321 cttatgcaga ctctacttca tttgttgtac cagataatga tcttcctgat gaaaaagttt
    34381 tatttctatc agatattttg cccacagctt attggagcgt tgaaaacgca ggagtaaaaa
    34441 aaggcgatac agttgtgatt ctaggttctg gtccagtcgg tctattcgct caaaaattcg
    34501 ctgttatggc tggagcagag cgcgtaatcg cagtagatcc agtacagcac cgtctagata
    34561 aagcggctaa ttacaatggg gttgaaactt atttcttaga agacacggca caagctggaa
    34621 cagaattgta tgaactggct aaaggtggcg cagatgtcat tatcgattgt gtaggaatgg
    34681 acggattaga gcctgtcaaa gaaaaagcga aaaatttagt aagcttacaa agcggcacaa
    34741 tctctccatt acagatggca tcgcaagctg tcaaaaaatt tggaacagtc caagtaactg
    34801 gtgtttatat gactccagct tcttcttacc cgctgcaaga attttttatg cgggatatcg
    34861 ctgtcaagca tggacaggca ccaattatcc atctaatgcc aaaaatttac gcaatgattg
    34921 cagaaggact attcgatcct acacaaatca ttactcattc gctgccacta tcagaagcag
    34981 cacaagcata tgagatcttt gacaaaaaag aagataaaaa tataaaagtt gttttgaaac
    35041 cttgaattca gtaaaaaata caggtgattt tctttatgcc caaaatcaaa cgaaataccc
    35101 atgagatcga aataggaaaa gaagaagata tcgaaatacg aggacgtcgc ttccgcttct
    35161 atttccaaaa tcgttacaca ttgatctcac tggctgtcga tttattaacc gggattttct
    35221 atatttttgg aagccttgct tccttaacta aaatccccga tcgatatggc acctattttt
    35281 atttggctgg tgctattttc ttgacgattc gtcctattct cagaatcgta aggaacattt
    35341 ttatttacga cgaaaaaaaa gcaatgaaga gaacaagcaa aaaaaaagag gcatgagaat
    35401 ttttcaaatc tcatgcctct tttttcaccc tatcgtatca ctttgataca gacgagcggt
    35461 cgtcccactt tcttccttga ccattgtcaa aaatgtccac tcgtatcgtt cataaaacgt
    35521 ggtatgatct gtaattaagt aaagtttttc gtacccaagt tgatgcattt cttttctgat
    35581 ttccgctaat atccgcttgg ctatcccctg cttacgcttt tccggttcaa caaacagggc
    35641 acaaagattt ggtgtcagat cagggcggtt atggaagtcg ttttcaatga cgcccgctcc
    35701 tccgacgatg gtttcttttt catctaagac cacataccat tgaggaatac tttccttatt
    35761 agcgattcca gtacgaatac tttcctcata cacaacagca ggaactcccc attttgatga
    35821 aaaccaattc gcgctttgtt ttactaattc tgggtgttct cttaatttga tccagttata
    35881 tgtaaccaat gtttaaacct ccgactaaaa taaaacgttt acaaaaatta tacaagtctt
    35941 tatgaaaatt agcaatgaca acaatagtga tgtaaaaaaa aaacggaaaa caatcacttg
    36001 ttctccgttt tttgattaat caagcatcta aagcttcttt tcttctttct tggatcgtag
    36061 ctaccttgac gaacggtaga tagatcaaaa tagaaagaac caaattgaca gcggctagta
    36121 caccacctgc aatacttttt gttgccaata caccgccaat aattggtgga gttacccatg
    36181 gaggcataac ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa gcaacactaa
    36241 ctaagaccat tggtgtcaaa ataaatggaa tgaacatgat cgggttcaac acgataggta
    36301 acccaaacat cgttggttca ttaatattga agacacctgg cgcaatcgac aagtttgtta
    36361 caaccatata tggtttgttt ttacgtccta ctaaataaat agctaacagt aaacctaaag
    36421 ttgcacctgt tcctcctaag ttgacaaagc tgtcaaagaa aggtttgttc acgataaacg
    36481 gtgcgctatg cccagctgcg attgcattaa cgtttgcttc aatggctggc gcattgattg
    36541 tttgcatcaa aggatcgacc atattcgctc cgtgtaaacc aaagaaccat aagaatggtg
    36601 tgataaatgc aagcagtaag gcagatggat aagagtttgc aagtcccata aatggttctt
    36661 ggaccactgt atagaaagaa cctacgatat catcaacacc gaatgcagca aaaattgcag
    36721 caactaaagc aaacgcagag atagtgatca ttgctggtag taatgaagca aatgcttttg
    36781 caactgccgg tggcacacca tcaggcattt tgatgatcaa tttttcattt ccgcttaaac
    36841 gtacaaagat ttctgttgaa ataatcccaa caataatagc aataaacaaa ccgtttgagc
    36901 tcatacctaa tgctccgcct aaagcgaaga aagagcctaa agaaatcaca cctgcggaaa
    36961 caccgtcttt tcggtagccg ttagctaaat gataagcaag caagaaagca atcaagacag
    37021 aaagaatggc aaatgtaccg ttccaagcag cagccccgaa tcctttccat gattcaccac
    37081 caaaaattga gttcatcaat tcttggaacc cgggaatcgg taagttattg atcattactg
    37141 ctaatgctcc taagatcatt aacggcatac tcaccataaa tgagtcccga atcgcaacta
    37201 aatgccgttg tgcaccaatt tttgatgcta caggtataaa atgtttctcc ataaatgcaa
    37261 caaatttatc cataaatctt ccctcttttc tttactgtag attacgcgtt caatcgacga
    37321 tacagatcaa tgatctcttt tgctaaatct gtaaaagcta tagatgtcat cagatgatct
    37381 tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc ttgtgtcagc
    37441 atctctgttt gagagtgatg tgcttggact aaagattcct ctgcatcttt gattttttca
    37501 tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg cttccattgc atcgcttttt
    37561 gcatttccgc caaacatgat cagacccatg atggcttcta gatttttttg atcttccatt
    37621 tattgtatcc tccgataatt tttattattt gtttattagt ttttctgctt ggtcgagtac
    37681 tttttcacca ttcatcatgc catagtcagc catattgatt acatctaaag gaatgccttt
    37741 aggtgctagc ttttcagcaa attgattttg cataaagcgt acttgcggtc ctaaaagtaa
    37801 aacgtctaca tttttagatt ctagttgatt atccgcatct gaagcagata cagcaaagat
    37861 gtctgcttcc attccttttg cttctgctgc cttttgcatt tttgtgacta ggagacttgt
    37921 actcattcca gctgaacata caagcatgat tgttttcttt tccattttaa acacgttcct
    37981 ttctatactt acacttgtat ataagcaagt agcgtgccaa ctttaaaagc gctttaaaaa
    38041 gttgatatca tcacatttaa ggtctatacc aagttgcata cacactaaaa aagaaagcgc
    38101 acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca caaaaaaagt
    38161 tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag cttgcatctg
    38221 cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg ctattcttat
    38281 tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa tcagctgacc
    38341 ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata accagtaaat
    38401 acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc aaatccaatt
    38461 ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt ctgcttcgtt
    38521 catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc attggtgaca
    38581 cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct tctgttgctt
    38641 tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct tgcacgattc
    38701 ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct gttatcagcc
    38761 aagggatatt atgataattt tcttttagat acatggctat atcatacatg attttcggat
    38821 agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag acttcataat
    38881 atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg cgaacgcgtt
    38941 taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc gtttctaaat
    39001 cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg aactcttctg
    39061 tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa tcatacatat
    39121 aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca ggatttaaaa
    39181 tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct ttagccgttg
    39241 ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca tgcggccagc
    39301 gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat gtaaaccagt
    39361 tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca taaaaatcaa
    39421 ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg acttcataat
    39481 gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc atttcactta
    39541 tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa aaacgagccc
    39601 aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc acatcctgct
    39661 ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc caaacaaaat
    39721 gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt cctttccaac
    39781 cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga atagtaattt
    39841 tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc tacttttaca
    39901 aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat aactgcaccg
    39961 atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca agggaccatt
    40021 acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt tccagtgata
    40081 agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg cataccaaaa
    40141 ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt gatttgtttc
    40201 atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt gactccagac
    40261 ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg tacttctttt
    40321 ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg ttcccgtatc
    40381 ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc aataaacatc
    40441 aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg tacgacattg
    40501 taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc cgcagccgcg
    40561 atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga aacattcgtt
    40621 ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca gcaataaagt
    40681 tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc tgtatattgt
    40741 ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc ataaacagtt
    40801 ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc atccacttta
    40861 ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa gaaacttagt
    40921 gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg caagaaactt
    40981 atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt gccaaaagca
    41041 cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat gatatacctt
    41101 aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc cataaaactc
    41161 attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt gtctattagt
    41221 cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca aatataaaac
    41281 aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa ttagtttctt
    41341 tttctcctat tattttctgt aattctctcc gatttattgc catatggaca gtcattgcat
    41401 caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta tgaatatcga
    41461 tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca attgattcat
    41521 taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca atcatcgtat
    41581 gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa gtttcaatct
    41641 tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc gctgccaaac
    41701 ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc atccgatcct
    41761 gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta tcactgacaa
    41821 atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc cgcacagctt
    41881 ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca ttcggcagtt
    41941 tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca gtagttactt
    42001 ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat gtattttttc
    42061 gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa ttgattaatc
    42121 cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg ata
//
LOCUS       DASJST010000005        39361 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJST010000005 DASJST010000000
VERSION     DASJST010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.005"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..1080)
                     /locus_tag="U4B05_000208"
     CDS             complement(1..1080)
                     /locus_tag="U4B05_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2049374.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1183..1623)
                     /locus_tag="U4B05_000209"
     CDS             complement(1183..1623)
                     /locus_tag="U4B05_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2049375.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(1680..2090)
                     /locus_tag="U4B05_000210"
     CDS             complement(1680..2090)
                     /locus_tag="U4B05_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2049376.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2270..2704)
                     /locus_tag="U4B05_000211"
     CDS             complement(2270..2704)
                     /locus_tag="U4B05_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2049377.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(2804..3652)
                     /locus_tag="U4B05_000212"
     CDS             complement(2804..3652)
                     /locus_tag="U4B05_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2049378.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4041..5807
                     /locus_tag="U4B05_000213"
     CDS             4041..5807
                     /locus_tag="U4B05_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN2049379.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            5909..7168
                     /locus_tag="U4B05_000214"
     CDS             5909..7168
                     /locus_tag="U4B05_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2049380.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFQKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7315..8616
                     /locus_tag="U4B05_000215"
     CDS             7315..8616
                     /locus_tag="U4B05_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2049381.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            8619..9470
                     /locus_tag="U4B05_000216"
     CDS             8619..9470
                     /locus_tag="U4B05_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2049382.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9483..10298
                     /locus_tag="U4B05_000217"
     CDS             9483..10298
                     /locus_tag="U4B05_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN2049383.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10449..11615
                     /locus_tag="U4B05_000218"
     CDS             10449..11615
                     /locus_tag="U4B05_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2049384.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            11845..12492
                     /locus_tag="U4B05_000219"
     CDS             11845..12492
                     /locus_tag="U4B05_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2049385.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            12576..13520
                     /locus_tag="U4B05_000220"
     CDS             12576..13520
                     /locus_tag="U4B05_000220"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN2049386.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            13655..15106
                     /locus_tag="U4B05_000221"
     CDS             13655..15106
                     /locus_tag="U4B05_000221"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN2049387.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15127..15963
                     /locus_tag="U4B05_000222"
     CDS             15127..15963
                     /locus_tag="U4B05_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2049388.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            15963..16622
                     /locus_tag="U4B05_000223"
     CDS             15963..16622
                     /locus_tag="U4B05_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2049389.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            16748..17437
                     /locus_tag="U4B05_000224"
     CDS             16748..17437
                     /locus_tag="U4B05_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049390.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17443..18399
                     /locus_tag="U4B05_000225"
     CDS             17443..18399
                     /locus_tag="U4B05_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN2049391.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18443..18955
                     /locus_tag="U4B05_000226"
     CDS             18443..18955
                     /locus_tag="U4B05_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049392.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19211..19759)
                     /locus_tag="U4B05_000227"
     CDS             complement(19211..19759)
                     /locus_tag="U4B05_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2049393.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(19848..21029)
                     /locus_tag="U4B05_000228"
     CDS             complement(19848..21029)
                     /locus_tag="U4B05_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN2049394.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21265..22773)
                     /locus_tag="U4B05_000229"
     CDS             complement(21265..22773)
                     /locus_tag="U4B05_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN2049395.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            22932..23123
                     /locus_tag="U4B05_000230"
     CDS             22932..23123
                     /locus_tag="U4B05_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049396.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23326..24243
                     /locus_tag="U4B05_000231"
     CDS             23326..24243
                     /locus_tag="U4B05_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN2049397.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24236..25309
                     /locus_tag="U4B05_000232"
     CDS             24236..25309
                     /locus_tag="U4B05_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049398.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            25457..25975
                     /locus_tag="U4B05_000233"
     CDS             25457..25975
                     /locus_tag="U4B05_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049399.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            25956..26348
                     /locus_tag="U4B05_000234"
     CDS             25956..26348
                     /locus_tag="U4B05_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN2049400.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26424..27359
                     /locus_tag="U4B05_000235"
     CDS             26424..27359
                     /locus_tag="U4B05_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN2049401.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            27492..28271
                     /locus_tag="U4B05_000236"
     CDS             27492..28271
                     /locus_tag="U4B05_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2049402.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28264..28581
                     /locus_tag="U4B05_000237"
     CDS             28264..28581
                     /locus_tag="U4B05_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2049403.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            28825..30435
                     /locus_tag="U4B05_000238"
     CDS             28825..30435
                     /locus_tag="U4B05_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2049404.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            30592..31440
                     /locus_tag="U4B05_000239"
     CDS             30592..31440
                     /locus_tag="U4B05_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2049405.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            31671..32150
                     /locus_tag="U4B05_000240"
     CDS             31671..32150
                     /locus_tag="U4B05_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049406.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            32183..34411
                     /locus_tag="U4B05_000241"
     CDS             32183..34411
                     /locus_tag="U4B05_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2049407.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            34540..36183
                     /locus_tag="U4B05_000242"
     CDS             34540..36183
                     /locus_tag="U4B05_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2049408.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(36256..36801)
                     /locus_tag="U4B05_000243"
     CDS             complement(36256..36801)
                     /locus_tag="U4B05_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN2049409.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(36824..37309)
                     /locus_tag="U4B05_000244"
     CDS             complement(36824..37309)
                     /locus_tag="U4B05_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2049410.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(37299..38111)
                     /locus_tag="U4B05_000245"
     CDS             complement(37299..38111)
                     /locus_tag="U4B05_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN2049411.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(38250..>39361)
                     /locus_tag="U4B05_000246"
     CDS             complement(38250..>39361)
                     /locus_tag="U4B05_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN2049412.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLQSLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        12958 a         7114 c         7732 g        11557 t
ORIGIN      
        1 ttaagggata tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc
       61 taaaaagaag gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc
      121 gctaacaagt aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa
      181 cttcggattc agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata
      241 aacaacatct cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat
      301 gataacacct gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag
      361 ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac
      421 aacaaattga gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta
      481 aatatcttga aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt
      541 ttttttcttt tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc
      601 catttttcct atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc
      661 aaaaaaatcg tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt
      721 tggctgatgc tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag
      781 catcgggata tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat
      841 gatagctaaa acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat
      901 catcaaataa gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa
      961 aagaacaaaa acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa
     1021 acgctgataa acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat
     1081 tatatgatgg aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc
     1141 ttatgagttg gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc
     1201 ttgatgaaaa ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg
     1261 ttcaagtgga agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc
     1321 agcaccttga gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg
     1381 attaatataa tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag
     1441 acacaaatca tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat
     1501 cacaagatac tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat
     1561 gatttcttta gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc
     1621 catttttcca cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc
     1681 tattcactaa ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga
     1741 cacactttat gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata
     1801 gatccttcaa tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact
     1861 tgtgcactga cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca
     1921 ctgttaaaac atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg
     1981 acgcgtttac caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct
     2041 ccagagcgtc cgcctgtgtt gatcattttc gtagaataca tttttttcat aaaattacca
     2101 cctttaatta tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa
     2161 tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc
     2221 aaacatatac aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt
     2281 tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc
     2341 ataaataacc aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg
     2401 tcatttcttg aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat
     2461 cagagacgat gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac
     2521 caagagacgt gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt
     2581 cattattgcc tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt
     2641 gatacagttc tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat
     2701 gcataaagat ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc
     2761 taaagaaaac gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat
     2821 aagtaaatcc gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat
     2881 cgatttcttc tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata
     2941 aaacctcttt tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc
     3001 caatctcagc acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa
     3061 cagatttctt tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa
     3121 tagataaaat agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa
     3181 cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa
     3241 tgaggggagg gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc
     3301 ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg
     3361 tttctggaac tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa
     3421 agggtttttt acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa
     3481 taactatagc tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac
     3541 ctccagcagc aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga
     3601 gggcaaaaat aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac
     3661 ttccttaatt cattaataat actcttatct tattataact tacttttcac cttaagttaa
     3721 ctttaattaa taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat
     3781 acttctacgc caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc
     3841 ttaagataaa ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt
     3901 aagtctatct atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga
     3961 taacattttt gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt
     4021 aaaacacaag gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat
     4081 atgcatttct atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata
     4141 tcaaaaaagt atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc
     4201 aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg
     4261 aagtgacgag cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca
     4321 cggactgttt ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac
     4381 caaattttta ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat
     4441 gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag
     4501 caaatgaccc cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt
     4561 tcgggggaga tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca
     4621 atggcatcta tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg
     4681 attattatga ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg
     4741 aagcacataa aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc
     4801 attcaccaca gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt
     4861 tccacatccg ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg
     4921 aaaatatccc atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa
     4981 atccagaagt gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata
     5041 ttgatggatg gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca
     5101 gagaagcagt tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct
     5161 ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt
     5221 cgattaaaga ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga
     5281 atcatcagca aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt
     5341 ctcatgatac tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg
     5401 tgatggcctt tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag
     5461 ggatgactgg tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac
     5521 aagatctaga gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta
     5581 agattatttc tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct
     5641 atttcacacg gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac
     5701 cttgggtagt agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag
     5761 acgggaaagc agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg
     5821 taacaacaag aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag
     5881 cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt
     5941 agggttaatc tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc
     6001 agcagaaggg gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa
     6061 agattatata aacgaaataa aaaacacttt ccaaaaagaa aacgatgtga aaatcaagct
     6121 agtagaaaag gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa
     6181 gggacctgac gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca
     6241 tctggcggaa gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt
     6301 gacttatgat ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta
     6361 taacaaagat ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa
     6421 agacagtcgt tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg
     6481 gactgacttt tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga
     6541 tgatggcaca aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc
     6601 ttatgcaaca gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc
     6661 aggagacttc gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc
     6721 atggcaagcg caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac
     6781 tctaaataat ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa
     6841 ttatgctaaa aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa
     6901 tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac
     6961 agcaaaggct aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca
     7021 agcgatgcca aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt
     7081 cgatgcagca tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat
     7141 cagtgaagcc atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa
     7201 tggaacaggt gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt
     7261 tgccattatc ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag
     7321 caagataaaa caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg
     7381 atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc
     7441 ctattcattg gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg
     7501 ggaagtatac caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc
     7561 atcattacaa tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta
     7621 gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat
     7681 ataggtgatg aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt
     7741 acgatcattt tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat
     7801 catattccac cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc
     7861 tttttaagct catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact
     7921 ttatgtgcaa caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag
     7981 aaatttatta aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca
     8041 gcttttatta ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat
     8101 actcaagtga ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg
     8161 aaaacggacc cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt
     8221 ccctatatct atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa
     8281 gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct
     8341 gtcttcctag tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc
     8401 tccatgatct atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct
     8461 actgatattc ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct
     8521 atggcttcag caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta
     8581 tttaaaaaga caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg
     8641 ctcgattcca aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag
     8701 tcattatcat ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg
     8761 cagcttttag tttagatctt tcaagtgact ggactttagg aaactttact cggctattca
     8821 gtgaaacact ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga
     8881 tcgtacaagt gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg
     8941 gccgcaaatc aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat
     9001 tgactgcctt ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga
     9061 caatgatcta tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg
     9121 acactgtacc aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga
     9181 tttttttcca gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg
     9241 cttttatggg accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa
     9301 atcagactgt tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct
     9361 ctctttttgc tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt
     9421 tacaaaagaa ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta
     9481 tcatgcaaac atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg
     9541 cgggacgcaa actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct
     9601 tgatggtcat gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac
     9661 aatttctatc cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg
     9721 aattatctga aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt
     9781 tggtaggtgt gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg
     9841 agaaggaaca atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga
     9901 attatagccc atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt
     9961 tagaaagaga attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc
    10021 ttggtcttgt cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt
    10081 ggatgacaag aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga
    10141 tgctgaatgt tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg
    10201 actttatctt gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt
    10261 ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga
    10321 caatgaatgt gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa
    10381 acttcttagt tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga
    10441 ggaaaatcat gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc
    10501 ctgtcgaagt aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc
    10561 cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgctt
    10621 ctttggcact aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag
    10681 cattgatcaa cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa
    10741 aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc
    10801 attatgactt aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt
    10861 gtgcttattt caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc
    10921 atatcttaga caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct
    10981 ggggaacatt gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac
    11041 taagtgaaga acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata
    11101 aatgccgtgt attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca
    11161 caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg
    11221 taaaagattt attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag
    11281 gcggtgcgac aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg
    11341 ttactgaatt agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct
    11401 tacgtcgtga ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag
    11461 atcaagtcac gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag
    11521 cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac
    11581 ctggaaacaa tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga
    11641 ctatttaaaa agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc
    11701 taggattgtc tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt
    11761 ctttaatttt taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat
    11821 tttataacaa aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca
    11881 gctttgtttt tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct
    11941 tccagtagcg aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct
    12001 tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct
    12061 gcagctcgtg cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg
    12121 gaattagata gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa
    12181 gaatacgaag caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact
    12241 ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa
    12301 ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca
    12361 acagattggg atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac
    12421 ggcaatcaaa gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa
    12481 caagatgatt aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca
    12541 ctagacaaaa taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca
    12601 atcatatctt tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg
    12661 aattagatga actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca
    12721 atgaacgaaa aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt
    12781 ctatgggact tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa
    12841 aagtgcagga aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg
    12901 aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa
    12961 atttatcttg tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt
    13021 atgaaacact gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc
    13081 cttattttga ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga
    13141 cctatgtcaa tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag
    13201 cagtagtcat caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga
    13261 ctgctttggc gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg
    13321 gtacaggtgg gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca
    13381 tgttacagat tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa
    13441 aagaattaac gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca
    13501 aattacgcac catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta
    13561 taaaaaacac tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt
    13621 gtgggaaaat aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa
    13681 agagcttaaa gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat
    13741 aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc
    13801 ccacgagcta ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt
    13861 aaaaatgctc ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga
    13921 aacacaccga gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt
    13981 tccttttgga caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat
    14041 ctatggccca gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg
    14101 agctgctgct gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg
    14161 ttctattaga atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac
    14221 gatgccagtt ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt
    14281 gacagtttct atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg
    14341 tgcgccatat caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa
    14401 gatagctttt tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc
    14461 tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta
    14521 cccactagat ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc
    14581 agcaatgatc cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga
    14641 acccatttct tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga
    14701 ttctctgcat gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga
    14761 tctattcctt tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag
    14821 tgatgcgata agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga
    14881 cgttgtttct tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa
    14941 tctgactgga caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct
    15001 aggaatccaa tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct
    15061 atttgaagca aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga
    15121 agaagaatga agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc
    15181 gcttgcggta cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag
    15241 aaagaattag tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa
    15301 aatgaattag ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc
    15361 aaaatctcct ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa
    15421 tcagattttg ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt
    15481 tcagagactt tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga
    15541 atccactcac ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat
    15601 atgcgtttag cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat
    15661 cagtatttaa gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg
    15721 cagaaaatgg ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat
    15781 ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa
    15841 atcgatggcg tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa
    15901 tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag
    15961 tgatgtactt tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc
    16021 aaggcttggt atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag
    16081 ggttgctttt gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt
    16141 tttatttatc gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg
    16201 ggttacctta tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca
    16261 gtgcatttat tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg
    16321 acgctggttt agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc
    16381 aagcttttcg aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta
    16441 cttcgttagc agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg
    16501 gtcggacatt cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt
    16561 gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct
    16621 gaaaaataga aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat
    16681 tctgcctagt attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt
    16741 agaaaacatg aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat
    16801 cgagcaaggc ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga
    16861 tacgcttagc tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa
    16921 agtgacaaaa gcagattttc gctctacagc caaactgtca tttgttggac attataatta
    16981 ttttgtttta ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg
    17041 tgttttagtg tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct
    17101 gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg
    17161 cttcatggct tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa
    17221 atattttcca acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc
    17281 attttctaac gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa
    17341 tgagttgcaa gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca
    17401 gcagatccgt cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta
    17461 agaggaaaac aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc
    17521 aataaaatcc aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc
    17581 gaattatttc aaaaaaagca ccatccaatc gctgtgatcc aaaatcccca agtaggacaa
    17641 ttcaaattat tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc
    17701 cctgcacaga tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt
    17761 cttgttttcg atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga
    17821 gttgattcga aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac
    17881 aaaattgtga tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa
    17941 acagatgact tttttagtga tgactatctt tttttccacg cagacggata tattggattt
    18001 tctgatttca cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt
    18061 gctatccatc taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct
    18121 gacagtaacg actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg
    18181 aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta
    18241 ttggctcttt accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt
    18301 atgcatcatt tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat
    18361 tattcaaaag ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa
    18421 ggaatagaaa agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga
    18481 agtggtagaa atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg
    18541 gcaaaacggg cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag
    18601 ctatgtatta atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga
    18661 tcccgtttac acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat
    18721 tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga
    18781 acatttgttg acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc
    18841 agctaatgaa ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat
    18901 tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata
    18961 aaatcatctg agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc
    19021 caagaggttg taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct
    19081 tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct
    19141 tcatcgagag gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta
    19201 ttcgatacat ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc
    19261 catcgtttga cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat
    19321 cttctacata aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca
    19381 ggcgcgattg caatgtttct ttagagatcg taataaagat aggacggact accgtcccga
    19441 aatactcata taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg
    19501 tcagtttctt tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc
    19561 actctgcaaa ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat
    19621 aatcctcttg atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt
    19681 gttccggctg aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa
    19741 atacatagca ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt
    19801 ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag
    19861 ccagtcacaa tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg
    19921 tgttttgcga caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc
    19981 ccgatagaag agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc
    20041 tcagtaaaat ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc
    20101 agtttagtcg ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg
    20161 aaggcaagag gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg
    20221 tggaagatag cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc
    20281 gtgatcgcca catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt
    20341 tctttgtttt cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca
    20401 attgaagcca gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta
    20461 gaatcgatca gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat
    20521 acactcatgg tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata
    20581 aaaacttccg attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc
    20641 atcccgttga ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg
    20701 atatagcgta gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga
    20761 aagaaaggtg cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca
    20821 gcaaaagcaa gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa
    20881 gttgtacgta ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt
    20941 ttcaatttga ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat
    21001 aaaccaataa tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat
    21061 tttacctgtt ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag
    21121 tgaaacgcat tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg
    21181 taacgtctta gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt
    21241 tcaacattta taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg
    21301 tgcgcaggta atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat
    21361 cacagtagag tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc
    21421 gattggattc gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc
    21481 atccaaagag gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg
    21541 tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac
    21601 tgccgctgac gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa
    21661 ttcgattgca atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc
    21721 atagtcatgg gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat
    21781 aatcagtttg attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc
    21841 tgtttggaaa taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc
    21901 cacgatccca aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc
    21961 tttaccagta attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt
    22021 atagttcaca gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc
    22081 ttttagatcg actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt
    22141 ccatactggg aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg
    22201 atacagttgg ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac
    22261 aacgattggt ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat
    22321 tttttctacg ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg
    22381 tctttctagt ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac
    22441 gacactgttg attaataagc cataaccacg taattcatcg attaaccgca agatatccat
    22501 atcataagta atcccataat caccacggat cttattacgt tcaatatctc cagcatagac
    22561 gcaaatcagt atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc
    22621 ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc
    22681 taaatataat ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat
    22741 atatttttct gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca
    22801 ttttttcatt tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag
    22861 aaaatggttt aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag
    22921 gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt
    22981 cgatttttac cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta
    23041 cttatctaaa cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa
    23101 caagagatct gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct
    23161 atcagatgga aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt
    23221 tttatggagc attgcccgct cttttttata acaatttttt acagaaaatg gtactattga
    23281 agtattaata agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat
    23341 acaaatgcaa attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga
    23401 caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta
    23461 ttagaagact tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac
    23521 cgtttataca atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag
    23581 atcgatcaat tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg
    23641 gaacaagtag aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat
    23701 gatcggccca cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc
    23761 gatgaagagt gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat
    23821 gagctagacg caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata
    23881 aaagaaatca atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa
    23941 aaaatcgagg aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa
    24001 ttaggctggc ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg
    24061 aagcaagaag caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa
    24121 atcagacgct atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg
    24181 aattatcttg gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa
    24241 tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac
    24301 gaacacttca ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat
    24361 gctagaggaa acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc
    24421 agaaaacacc catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca
    24481 agatcatcaa gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg
    24541 tatgagaaaa gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt
    24601 tatattgaat gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac
    24661 gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga
    24721 cacatggaaa acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt
    24781 cttttccttt gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct
    24841 aaacgtatca ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat
    24901 ccgacactgg tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa
    24961 attgaatcaa ttattagccc atgcaagaac acttggtttt accgttaatc agacattact
    25021 agacaatacg aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt
    25081 attggatgat atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa
    25141 cgaaactact tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga
    25201 aaaagatcaa acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt
    25261 ctttatcaat tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt
    25321 ggattaccag atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa
    25381 taattgtcac aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg
    25441 agggatttca ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc
    25501 ttgtgtagca ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat
    25561 ttttttattt atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc
    25621 tattgcgatc agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag
    25681 ctatactgtt gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa
    25741 tctatggtta aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac
    25801 cgtttttgat acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt
    25861 gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt
    25921 cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc
    25981 ttgcaatgag tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg
    26041 cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa
    26101 atggtcaaaa cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta
    26161 ttttgaaacg aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc
    26221 actcacagga tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta
    26281 ctactggtgc caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta
    26341 aactataagt ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca
    26401 gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa
    26461 ttgctgctct aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca
    26521 gtggcagtgc cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga
    26581 ttttgctgtt attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg
    26641 gtgaaggacg agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg
    26701 gtggggcact tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg
    26761 gtaatccgat cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt
    26821 tacgcgtggc aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct
    26881 ttttgcgcga aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg
    26941 taatcggttt ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg
    27001 catttgatgc gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc
    27061 tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa
    27121 aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc
    27181 atttaagtcc aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga
    27241 atgaagctta ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca
    27301 agaagatgta tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa
    27361 acgtttattc atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac
    27421 ggctacaatt tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag
    27481 gagatatttt aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga
    27541 tgccactttg tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag
    27601 ttggatttga tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc
    27661 agtttttagt tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg
    27721 ttttcttttt agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa
    27781 aagaaaagcg gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg
    27841 tgaactactt aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca
    27901 attggttctc aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg
    27961 tgattcgagt agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt
    28021 ttatcatgca gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag
    28081 gggtcgtatt catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg
    28141 cttcatttgc cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa
    28201 gagaccgcgg taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc
    28261 accatgacta aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc
    28321 ccgcgcttgt tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac
    28381 tggatgaaat atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt
    28441 actcatgaac atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca
    28501 ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc
    28561 tttttgctgt ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag
    28621 ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat
    28681 tcgtagggat aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa
    28741 ttagagaggg atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat
    28801 gaaagaatag ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta
    28861 acctttgcta atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt
    28921 atgatgggga acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt
    28981 ccttatggat tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa
    29041 gtagctgtag ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg
    29101 atcaatatca tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct
    29161 ttactagccg gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt
    29221 ttatgcccat ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga
    29281 aaaaatgatc tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc
    29341 gttatcttgg ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct
    29401 gtattgatca gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat
    29461 atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat
    29521 ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt
    29581 tttgtcggct cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg
    29641 cttcactttt tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct
    29701 tctgtcaatc caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata
    29761 agcagcttgc caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca
    29821 gtaggagata gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct
    29881 ttattagctg gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat
    29941 ttccaaatgg ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg
    30001 cttcaatctt tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg
    30061 aaagtcatct tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca
    30121 aatggcttag cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac
    30181 cgaatagcac ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg
    30241 attatgcttg cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa
    30301 tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt
    30361 ttattttctc aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc
    30421 agaagcaaag aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt
    30481 atgattacgc ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat
    30541 gatatagtta taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt
    30601 gcagtagtta cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta
    30661 ttcgttattc caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc
    30721 gaagcggatg aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa
    30781 cctgtcgtag gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg
    30841 attttaagta tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg
    30901 aaagatgaca tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg
    30961 atgggacaca tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa
    31021 atacttgcac atatcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat
    31081 ttgaacaatt tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta
    31141 ttaaaaatca aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc
    31201 cgttcaagta aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa
    31261 atacaaccgc cagtcaaact ttacgtgata catgcaaata atttagaagt agcgattgcg
    31321 gagaaagaga aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga
    31381 ccagtcattg gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa
    31441 ttccttttat tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg
    31501 ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat gaagctcttt
    31561 ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa
    31621 actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata atggaaatac
    31681 caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa
    31741 gaaatgcatt aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc
    31801 gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac
    31861 agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa
    31921 gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg
    31981 tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa
    32041 agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg
    32101 gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga
    32161 tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc
    32221 acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga
    32281 atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa
    32341 cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc
    32401 tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt
    32461 tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag aattactaga
    32521 agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg
    32581 cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga
    32641 ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa
    32701 aggatattta ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc
    32761 acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg
    32821 ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt
    32881 aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga
    32941 agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa
    33001 gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt
    33061 agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact
    33121 ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat
    33181 tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa
    33241 agaaaaatgg ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga
    33301 agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa
    33361 atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa
    33421 aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca
    33481 aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca
    33541 tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat
    33601 tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc
    33661 acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga
    33721 gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt
    33781 caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca
    33841 tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga
    33901 tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt
    33961 cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc
    34021 tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac
    34081 tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt
    34141 aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc
    34201 gttgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata
    34261 tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa
    34321 gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca
    34381 aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc
    34441 agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc
    34501 ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg
    34561 tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat
    34621 ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc
    34681 ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta
    34741 agtgactatc aagatataga agagagattc ggaacgatgg aagattttga tttattgcta
    34801 aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg
    34861 gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat
    34921 tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc
    34981 ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa
    35041 gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc
    35101 cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa
    35161 aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca
    35221 ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact
    35281 gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt
    35341 tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg
    35401 caattagatg tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc attggatggt
    35461 atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt
    35521 ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc
    35581 tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc
    35641 tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt
    35701 gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac
    35761 agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct
    35821 tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca
    35881 gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg
    35941 atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga
    36001 tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat
    36061 gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga
    36121 gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag
    36181 taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc
    36241 tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg
    36301 aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac
    36361 atgtttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac
    36421 gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc
    36481 agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg
    36541 ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt
    36601 aggatccaac aactctttta acgtacggat gatccattga ccaacttccg cactggaatt
    36661 ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc
    36721 ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa
    36781 ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg
    36841 cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt
    36901 ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg
    36961 cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac
    37021 gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg
    37081 aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac
    37141 caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc
    37201 ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa
    37261 ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct
    37321 cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta
    37381 gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct
    37441 aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta
    37501 gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca
    37561 gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca
    37621 gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa
    37681 agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg
    37741 atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca
    37801 gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt
    37861 gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc
    37921 gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta
    37981 atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa
    38041 gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag
    38101 attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa
    38161 aacagccaat tggcatattt ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga
    38221 ccaaaatgga tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg
    38281 atttgaagcc gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg
    38341 atagggacgt tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt
    38401 tctttatcag gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc
    38461 actacttctg ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta
    38521 ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc
    38581 ataaaaccgt aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca
    38641 tcttccttgt aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg
    38701 ttttcaggta ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt
    38761 ccaacgccca tatctcttgc atcacttgtt ttcaaactct gtagtgtatg agccttaaga
    38821 atcattggtc cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc
    38881 gacgttcgca aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag
    38941 tatcggatac ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag
    39001 ttattcggat gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt
    39061 gaactttgtg cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata
    39121 ccaatattga atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa
    39181 atttttgtga aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga
    39241 caaataagtt tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt
    39301 ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagga
    39361 a
//
LOCUS       DASJST010000006        38091 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJST010000006 DASJST010000000
VERSION     DASJST010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.006"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(307..1617)
                     /locus_tag="U4B05_000247"
     CDS             complement(307..1617)
                     /locus_tag="U4B05_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2049413.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1629..3047)
                     /locus_tag="U4B05_000248"
     CDS             complement(1629..3047)
                     /locus_tag="U4B05_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2049414.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3287..4855)
                     /locus_tag="U4B05_000249"
     CDS             complement(3287..4855)
                     /locus_tag="U4B05_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2049415.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4859..5269)
                     /locus_tag="U4B05_000250"
     CDS             complement(4859..5269)
                     /locus_tag="U4B05_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN2049416.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5266..6189)
                     /locus_tag="U4B05_000251"
     CDS             complement(5266..6189)
                     /locus_tag="U4B05_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN2049417.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6186..7064)
                     /locus_tag="U4B05_000252"
     CDS             complement(6186..7064)
                     /locus_tag="U4B05_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN2049418.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7051..8388)
                     /locus_tag="U4B05_000253"
     CDS             complement(7051..8388)
                     /locus_tag="U4B05_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2049419.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8391..9494)
                     /locus_tag="U4B05_000254"
     CDS             complement(8391..9494)
                     /locus_tag="U4B05_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2049420.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9568..11076)
                     /locus_tag="U4B05_000255"
     CDS             complement(9568..11076)
                     /locus_tag="U4B05_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2049421.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11042..11734)
                     /locus_tag="U4B05_000256"
     CDS             complement(11042..11734)
                     /locus_tag="U4B05_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2049422.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11756..12730)
                     /locus_tag="U4B05_000257"
     CDS             complement(11756..12730)
                     /locus_tag="U4B05_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2049423.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13030..13902
                     /locus_tag="U4B05_000258"
     CDS             13030..13902
                     /locus_tag="U4B05_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2049424.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14360..16558)
                     /locus_tag="U4B05_000259"
     CDS             complement(14360..16558)
                     /locus_tag="U4B05_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2049425.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16790..17782
                     /locus_tag="U4B05_000260"
     CDS             16790..17782
                     /locus_tag="U4B05_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2049426.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17842..19263
                     /locus_tag="U4B05_000261"
     CDS             17842..19263
                     /locus_tag="U4B05_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN2049427.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19256..20755
                     /locus_tag="U4B05_000262"
     CDS             19256..20755
                     /locus_tag="U4B05_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN2049428.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20794..22188
                     /gene="uxaC"
                     /locus_tag="U4B05_000263"
     CDS             20794..22188
                     /gene="uxaC"
                     /locus_tag="U4B05_000263"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2049429.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22347..23030
                     /locus_tag="U4B05_000264"
     CDS             22347..23030
                     /locus_tag="U4B05_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN2049430.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23032..23868
                     /locus_tag="U4B05_000265"
     CDS             23032..23868
                     /locus_tag="U4B05_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2049431.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23879..25633
                     /locus_tag="U4B05_000266"
     CDS             23879..25633
                     /locus_tag="U4B05_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049432.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25909..26250)
                     /locus_tag="U4B05_000267"
     CDS             complement(25909..26250)
                     /locus_tag="U4B05_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2049433.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26318..28489)
                     /locus_tag="U4B05_000268"
     CDS             complement(26318..28489)
                     /locus_tag="U4B05_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2049434.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28658..29557
                     /locus_tag="U4B05_000269"
     CDS             28658..29557
                     /locus_tag="U4B05_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2049435.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29628..30485)
                     /locus_tag="U4B05_000270"
     CDS             complement(29628..30485)
                     /locus_tag="U4B05_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2049436.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30470..33172)
                     /locus_tag="U4B05_000271"
     CDS             complement(30470..33172)
                     /locus_tag="U4B05_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2049437.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33185..34474)
                     /locus_tag="U4B05_000272"
     CDS             complement(33185..34474)
                     /locus_tag="U4B05_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN2049438.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34624..35670
                     /locus_tag="U4B05_000273"
     CDS             34624..35670
                     /locus_tag="U4B05_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2049439.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35672..37825
                     /locus_tag="U4B05_000274"
     CDS             35672..37825
                     /locus_tag="U4B05_000274"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN2049440.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11246 a         7831 c         6775 g        12239 t
ORIGIN      
        1 ttgtcaactc ttttttaaaa gtttttttcg atttcgtttt tcgaaatctt tcagcaactc
       61 atttatcata acttatcgtt tgttatttgt caagaacttt tttgaagttt ttttcgatag
      121 aatcaagctt cgcaagtggt tctttttaac gacttagtta tcttatcacg tctaaaaaca
      181 tgtgtcaact cttttttgga attttcataa aatgtttcaa tgaagcgcga caacgttgtt
      241 taatatatca ttaaaaaaaa taaaggtcaa gctaaaatat tatttaagct tgaccttatt
      301 ataatttcaa cgattgtcaa aagtattgtt tgcagctgtg ttgccagttt ctatagataa
      361 atcacagctt ttgaaacata agttttgtac tgcttcgatt cgcccgaatt cttttgcagt
      421 tatttggcaa tgagaaaagc agacatcttc tataggcttt tcaggaaatg ctactaaatc
      481 aaaagctcgg gaaggaagtg agtaacctgg agaaaggcat gcctgtacgt ttttgatgtg
      541 tatgtctttg actttcgtca tttgctttga ttcaggtatt tcttcagcca ctgcttgcca
      601 actttctggc atttgagcaa ggtcattgct attttttcta ttgtattcat tatgccagtc
      661 cattatccaa gaaaaaggaa attgtacatc aatcatattc agattttcta ccctgatatt
      721 ttcgatcact ccccctcttt cttttgaaga tttcattcga ataccacagt ctgactgaaa
      781 aaaatcgata tcgtgaatat atacgtcact aactccagca ctaacttcgc taccaatagt
      841 tacaccgtat cctgaacgga ttttacatct agcaacctcg attcttgatg attttcgatt
      901 gactcttgca ccattgccat ctctgccaga cttaatagca atacaatcat caccacaggc
      961 caaatcacat tcatagacac gcacatttgt tgaagaatca atatcaatcc catctgtact
     1021 tggtccatca ttgttgcgga tgattacttt ttctactaat acatcgttag aataagtgat
     1081 ttgcaagttc caaaaacctg atttctgaaa aatcaaatca cgaacaacaa catgttctgc
     1141 atgatacagt aaacaagcgc gtggacgttt aatagcataa tccgcaatcc aacgaagtcc
     1201 cttttgatcg tattcagctc ttgtcccgct tttttgatct tttccccaat acagctccca
     1261 ccaatggtct ccttgaccgt cgattattcc ttttccttct ataagaatat ctttcgccga
     1321 gagaacattc aaaatcgctg ctggccattt catttctacg ccggctactc gtgtatcgat
     1381 ttctggaaat gcttcgatat ctgttctccc cttgaggact gcaccttctt caaagataag
     1441 atgactctta tcttttaaaa aaagggctcc gactaaataa atgccttttg gaataaatac
     1501 aggtaagtgt tctttcgcac cttgatcgat tgctttttgc agtattattg tatcatcttg
     1561 ttgagtgtcg tttgttttaa agttcaaagc attgataatc cctttttttg tccgcaatac
     1621 gtttctccct atctttcatt gatttttttc aattcttcac caataaatac agtttttgaa
     1681 cgattcagtg ggtaacctac tagcaaaatg attgcggata caaataagca gaatgctggt
     1741 aacaagtttg ccatcgtata aatcctttct tgtactaaaa ctgattggat ggctccacct
     1801 gttgtagacg attggtaacc aatgagtgcc agcataaatc cgcctaccgc tcctgcacat
     1861 gcttgtccta cttttctcgc aaaagaattt actccatata ctgttccatc ttctcttaag
     1921 cctgtcttat attgatgata atcaatcacg tcagtaatga atgcccagac catcaaattg
     1981 aataaaccag atcctaaagt agcgataaat aaaagaatca gatataccca tgcatttgtt
     2041 atatgaagga aatacatcag caagtacata catgaagaag caagtaacgc aaatacgctt
     2101 gcttcctttt taccaaataa ttttgtcagc tttgtagcaa atggtgctaa aactactact
     2161 gttccgtaag taaataaaag agcaacagac attgcttgtt tattatggaa gtaatcattg
     2221 aataaataag tcatattcgt cccagctaaa atctgattga tcacaatgaa aatgtcaaca
     2281 atgactaaaa taatcagtgc tcgattagct aacaaacctt ttaccaactg agaagcagat
     2341 actttttcgt ttttgacaac tcttactctt tcagtagtca atttacaagt taacatatat
     2401 gcgacaaatg ctattgcagc acatccgatt gaaatccaaa agaaatgatt ccctgaaacg
     2461 acttgttgtc cactcttgtt atgggtatac attaaaatcg gaatgaagaa tcctgtagtt
     2521 gatccgccaa tcgcagaacc aatgcttcgg aacgtggaca acgaggtgcg gtcattagga
     2581 tttgagctaa ttgctgctgc cattgctcca tacgggatat tgattgttga gagaaaaatc
     2641 ccataaacaa tgtatgaaac aaaaatataa gcaatcttca ccgtcattgg aagatgttgg
     2701 acgaatggca aaaacaaaat gacagtcact aagcaaaatg gatacttcat ttttctgatc
     2761 caaggaataa atcggccagt ttctgttaac tctgagacat cacataatct tccaaccatc
     2821 acatcagcaa acgcatccaa aattctcgaa atcaagaata acgtacctac gattgcccct
     2881 gaaacgccaa gaacattcgt ataataaatc atcaagaaac tattgactaa acctaaaata
     2941 aaacagttac ctagatcacc gaacatgtag cctatcttat cggaaatccc aaattccttt
     3001 ttttcttttt ttattgtatt tgctgtaaca gcattttgtg aaagcatttg aattcctcct
     3061 acctcttcta gtaaacgttt tcaaaattat aactcttttt ttcttgtttt tatattcaca
     3121 atgttggctt tttattgcat tatcttgaca gataaaaaac aaaaaataat ctaaaaacat
     3181 tgatgcataa gattgataac tttttttaca taacaatata tacaaacaaa aaaattcact
     3241 aactagtgaa aataagttag tgaacctcat ttattaaaga attaaattac agatgatctt
     3301 ttccatcata cttgaaaaac tcaaaatcag cataacttcc tcttttttta tttagatcat
     3361 gcactgctat cccgataaaa tttccagtaa accctcctga gagtatacac atgttttttt
     3421 gcggacctac tttcttccat tcttcagttg atacgtcttt caaatgaaac tgtgctgtct
     3481 ctttctttac agtgactttc aaaaaagtct tcttttctgt gagtggaatc gtccaatctt
     3541 ctatttgtgc tactccgttt tctgattcta tcatacgtag gcattttccc ttgtcctcat
     3601 tccaagtaac ataacaataa atataattgg attcatttaa gtacagtacc agtccagcca
     3661 tctgattaaa attattaggc atgtattcaa atgctgtctc cgcatcaaaa gtaaaatccg
     3721 tttgccgaat agctaacaaa tgatgttcga aggtagattg aagagattca ccggaaatca
     3781 gacgtaaaaa acctggtcta cattttgtat cacaccaatc agcatttggc aaaatgcgtc
     3841 tggcattcca gttcttcttt agctctcctt caaatgtatc cataaactga tgatcttttt
     3901 cttgttgtac ctcttcattt gtctcaatca ttatttcttc catcggagtt gtctggctat
     3961 ctttaagtct taaccaatcg tcggacgtcc aatacacttc ttgaatagcg gtttctcttc
     4021 ccaaaatagc cgcaccgttt aatggtctgg tacaaagata tgtcatgtac catcttcctg
     4081 acggggtttg tactaagctc ccatgtcctg tacattgcaa tgtagaagca ggtttgtcac
     4141 tagctgtcag cataggaaaa ccaggatcaa gctcgtaagg accagtaata ttctttgctc
     4201 ggcaaactgt aactgaatgt cctcttcctg taccgccttc tgccgtcatc aaataataat
     4261 acccgttacg aaaatacaaa tgtggtgcct ccgttttcgc taattctgta cctgcaaata
     4321 ttttatagat gggaccatca agcttcttct tctctgcatc atattcttgt aatacgatac
     4381 cgctggattt attcggtgtg tccagacgat aatcccatag ttcattgaca agccattttt
     4441 ttcccgtttc aagatcatgg aacaaagaag gatcaaagcc gttactattt aaataaattg
     4501 gttcggacca aggacctgtg atctcagatg cagtgatcaa ataattgtgg acatctttaa
     4561 acggacggcc tgcacttttg acatctgtgt aaacaagata aaacaatcca tcagcataac
     4621 tgatttgtgg cgcccatata cttccatttg taggatttcc ttgcaaagat acttgagtgg
     4681 tcaaaatatc cgttgtatgt gaccagttga ctaaattttt cgactcatat actcggattc
     4741 ctggaagcca ttcaaaagag gagacaataa tgtaataccg ctctttcact cttaatatat
     4801 taggatctgg attgaatcct cttaaaatag gatttttcac tttgatagtc tgcatgattc
     4861 aggctccttt tctcattctt actcgatata aactgaatgc cccagctgcg aaataaaaaa
     4921 atgcaaaagg aaacgcaata aactgagtgc caatcaacaa tatgaatcct gttataaaat
     4981 acaaaccagt ggcagttgtt aagtattttc ctttagcagt caaaaacaat gctactgcta
     5041 aaaaagcaag tgaaataaac aacacaacgg caaacagacc agtcatgtcc tgtaataatc
     5101 caaatgtatt ttctaaagac aaagttgaat gatctacagc aagcgactga taactttttt
     5161 taaactctga cgcagacata ttacttaaag taactgctaa cccgccaaag aaaatcaaag
     5221 atagactgcc accgaccatt cctagcaata gttcccattt tcttttcatt tctgaacccc
     5281 ctgcttttct aaatgtatcc attcaggtct tttgatcgat gtttcataat tgttattacg
     5341 ttcagagaat ttgacagtct gttcattttt cttgtcatcc caattatccc aaccagttgg
     5401 gtgaacatgc ccacctatct tacacgcttc aaataagaca tttgcatacg gacgccacgg
     5461 tcttcctaaa tagacgtttc tagtatcagg ttctgcagtc aaggtacagc tttcaaaacg
     5521 ataccctgtt ttttgatttt tcggcgtaga tgctgctgtg atgtatccag gatttttact
     5581 ggctaagcta tggatatgac actgatgaaa aacggcatct gccccaccaa atatgaaatc
     5641 caccgttcct tcaatccagc aatattcata ggtctgacga caatactcag gattggcaga
     5701 agccggtgta caaaaaggag taccatcttt ttgcaaatca ggcaacggac ccgtgcataa
     5761 ggtatcctga taaccagata gtcgacagtt ttttaaagat acgcgatctc ctgaattgaa
     5821 taaagcaaca gcctgtccta ccttttctcc tggacctgct gtattttcga tgtgaagatt
     5881 ttctaatacg acattcgatc cttctaagaa aaaagtagca cttcggaaag tccctaactg
     5941 gctaccgtca agatactcct gttttgcaaa acgattaccg gagataacta ctgttcctgc
     6001 tcccacaagt ttaaaattgg ataatctgct ttctatatat tcatcatatc ttccttcttt
     6061 tactatcaag acctttggat ggctatccgg caggcctgcc aaataatcga ttccttcttg
     6121 gatagtttca aaatccgtcg gggtatctat agcattcttt ccaattatca gtttgtcttt
     6181 catcctcata aatgctccct tatgaattgt ttccaaatat ttctcatttc ttgtgtttcc
     6241 atatgaccgc ctgttaaaga aaaagaagaa aagttttctg gactacccat tgattgatac
     6301 tttttgtcca gcccttttct cagcttcagt acgccttcgt ccaagcacat cgtatcgttt
     6361 tttccaacta agctaagacg agggcgcgga gcgatttttt cttggattgc taacgttgta
     6421 taagctgtca atagatttgg cacatagtaa taaaacccat gatgatctag gcggcgatgt
     6481 agcaaaagta tttcgatgtc cacctgtgcc gctagatcaa tacaaacttt gacacgagaa
     6541 tccaatgctg tcacccacca gcttaataag cctcccatag aaagaccaat cgttgcgagc
     6601 cgttgagtat ctgtgtcttc ccttgtctcc aaataatcga tcaagctgat cacatcataa
     6661 atacgcattc cccataacgt ttttcctgtt aataaaaatt gtttgaacaa ctcgctttca
     6721 gaaatacctc cgcgttcttc aaatccccaa gcatcaatcg accaaacagc atatcccatt
     6781 cctgtcagta cttctgcaaa agatggagaa gctaagtaag gagcaccatg gatcaattca
     6841 cttttcccga ctgaaaaatc tccgccgtga gaatgcaggt aaaggaccgg cggaaacggt
     6901 ccttcttttt ctggataagc atagagggct ggtactgttt ccaattcatt taagtacaat
     6961 tgatagtatt caagcataaa accgttttgt ttttctcggt ataatcgttt tccactaggg
     7021 gtatgtctgc tagatggagc acctaaaaaa tcattcaact gcataagcaa agtttttacg
     7081 aacaaccagt tcatccatat tttctgattg atttgattgg aatttctcaa tactgatttc
     7141 tttagtattt tctaatagaa atgctggtcc ctcgtgattt tcaatcgaca tctggttaaa
     7201 ggaaacctta cccgcaaagc caatataaaa tccttgtctg ctcatttcct ctattcctgt
     7261 catcatagca ggaacaccgg gaactgcatc ttgggccatc gagacatcga tattgtcaaa
     7321 tgtgatttct tgtatgaatt gttccgctaa tccataaata aaacctgcag aagcatggac
     7381 attccttgcc gtgatattcg aaaaatggat ccttctgaat gctggtgtcc gttcatctat
     7441 cggataagcc tttttttccc atacatacgg ttctttccct cgcggaccac agaaataata
     7501 aagattcagt ataaatggac acatcacatt atccatgact atattggata ctcgaatatc
     7561 ttcaacgatc ccgcctcgac ctcgtctaga tttcaatcgt attcctcgat cagtttcttg
     7621 gaagatacaa tttgaaatgg taatattacg aatactacca ctcatttcac ttcctaatac
     7681 gacaccgcca tgtccatgga ccatcgtgca atttgtgatc gtgatatttt cgcaagcgat
     7741 cctttcgtac gtatcttcag tccctgcctt gattgcaata cagtcatctc ccacgtcaat
     7801 atggcaatta ctaatacgca catttttgca tgattctgga tcaattccgt ctgtatttgg
     7861 tgaatccgct gggttcaaga tcgtcaagtt atcaaacgtt gcattactgc aaagaatcgg
     7921 gttgatcgtc caacttggtg actggatcaa tttaatatct ttcacagtaa ttcgatgaca
     7981 gttatggaaa ctcatcaatt taggacgagg ataagcgaga ttatctggtt catttctaaa
     8041 agtatgccac catttttttc catttccatc taatgtaccg aaaccagtga cagagatgtt
     8101 ctctacattt tgagcataga tacaggaagc ataaactttt cgatggactc cttcccaacg
     8161 tgaatgaaca acaggataat cttttggatc gtcagagaat ttcagaaccg ctcctgcgct
     8221 taaatgaagt tcgacattgc tttttaaaaa taaagctcct gttagaaatt cacctgctgg
     8281 aacaactacg gtcccgcctc cgtcacttgc tgcagcatca atggcttgtt gtattgcttc
     8341 agtattcaat tcatcaattg atgcgccaaa tgtcaaaata tcatacatat ttataatgcc
     8401 tccacttcaa tcaatgcttg caagaatgca ccaacgcctt tttgatcatt acaaataatt
     8461 ggttcactga tgtagtaagc atatgaacca tctcgctgat ctgctccgcc taaccctgct
     8521 acttgacagt ttttattcaa gttcagccag ccttcttttg tttccagaac aaattcatca
     8581 agtaatcctt gatgtgattt cttcacttgt ggaagccatt cttctttgtt cagcagtccc
     8641 aaacgtattc ctttagccat tgcgtaaaca aacatactac tgccggaagc ttccagataa
     8701 tttcctttcc gttttgtttc tgttgttact tgataccatg tcccgctgtt ttcttcttga
     8761 tatggaagta atgcagccag tgtcttttct agaatttctt tcagcaaaga acaatctgtt
     8821 tcttcatgaa gtaattcata ggtatctgct gcagccatca aataccagcc aatcgatcgg
     8881 ctccaaaaat gatgggatag acctgttttt ggatcagccc atggttggac cttcttttca
     8941 tcccatgcat gaatcagcaa atttgttttt ggatcgaata gatgtttata actgatttca
     9001 aactgtctca aaacatcttg gtaatctttt tcttcactaa aattctggat aaattcagca
     9061 taaaaaggag aacccatata taagccatcc aaccaaattt gataaggata aatttcttta
     9121 tgccaaaaag ctccttcgga tgtgcgtgga tggtttgcca actgctggga aaggagttct
     9181 gctgcttttt tatatttttc ttcgcctgtt tctttgtaca ataaaaagaa aagctttcct
     9241 gtatttacat ggtcgatatt gtattcttca acgctgtagc cacggatcgt tccatcttct
     9301 tgcacgaaat agtcgatatt gtcttttatg aaatcaaaat aaattttttc ttgggttcgt
     9361 tcccaaagta attggaaccc tttaagtacg acgccatagt cataagacca ttttccttta
     9421 taccactttt cttcatataa gcgaggtgtt ctttccatga tactttttgc taatttcttt
     9481 gaccagtttt ccattatttg ctctccttta aaaattaaaa taggatttgg gacaaatacc
     9541 aagaagtatt tgtcccaatc gtctatatta ctgtgccgct gctttgtatg cttcctcgta
     9601 ctcttgtgtg attcgatcgc ctccttgtgc tttccagtct tctacagctt ttttgaatcc
     9661 atcttcgtca agctgtccca tgatgtattg caccgtagca tcaatgacga ttttttcaat
     9721 ttctgatcct acttcattgt tcgtcggtga atccaatggc acagaaggat ccattacagc
     9781 aaattcttca ttttctttga tcaattgatt agagaattct ttttcagggt ctgtatcttt
     9841 gaaagagaac gtcacttcac taggacgaga gcttgataac ggttgcacct cttgttgcca
     9901 aagatccata tcgataaatt cgatagaacc atcatcattc aattcataat gcgttccttc
     9961 gataccgcca gtcatcaatt tatacacatc ttcttctaaa agatcatcaa caaattgaag
    10021 taatttcttc aattgtgctt cgtcttttac ttgagatttg ggaaatgcta gcaagccgcc
    10081 aataccattt ccttctgacc aaacatgata accgccgcct tcaccatctg agatcttgtt
    10141 tacaggcact aattccatat catcttgaat accttgaccc atcgtcttca gattcttgat
    10201 atccaccatt ccagtgtaga ttccagttct tccttgtgca aattgttctt gctgatcagt
    10261 cttttgggta actgcaaagt cttgtgccaa atagccattc tcaaatagat ctcttgaaaa
    10321 attcaaagct ttcatatact cttctgtatc aaattctgga gtgaatttgc cattatcgtc
    10381 tacagcccag ccatttggtg ctccaaaata agaagtgaaa agtttaaagc tggtataacg
    10441 aatatcacta ggcgcacgat cgccaaaacc aactgtgtcg tccacaccat ttccatcagg
    10501 atcgttttct gtaaattgtt tagctacttc ataaagctca tccatggtag ttggaacatc
    10561 tagtcctaga ttatccaacc agtcttttcg aatgactaag ccagcacgag cgtagtcttt
    10621 ttgtatcggt acgccgtaca aggcaccgtc gatactcgca gcttcgattc tatcttcaga
    10681 aatttgactt aaatttttat agtcttttaa atattctgat acatcccaga atacgcctga
    10741 ttttaacgca ttgcggacag aagagttttt caacatcgca atttgaagtg taacgatatc
    10801 tcctagttca tcagaagcca atgcagttgt taatctttct tcttttgaag catcaggaat
    10861 ccagttgaat gtcagttccg tatcggtttt ttcttcgatc aagttcacaa tcgtgtctgt
    10921 aggcggtgaa gcatgatgca aaatatttaa ccaagtgatt ttagcttttc catcttctgt
    10981 ctctgaagaa ctgttacttg aactgctgcc gcatgcggca agtgctagaa ttcctgcaaa
    11041 actaagacct aattttacga gcgttgcttt tttcataaat tttctccttc actgttttaa
    11101 taatattttt gttttcttct gttttttctg tatatgagtt cttccattcc aaatggcaaa
    11161 atatagaatt tgctgtccac gtgactgcgg aaaagaagag actgccaccg ataaaggtta
    11221 aaacacctgg cattgtttgg atgatcatca gataaatccc taccatactt aatatcaaca
    11281 ctaatgagta atgaagatgt gagaatccga ataaaaatgc cattttcatt ttgaaaaaat
    11341 atcctttcca atcataatga gccatgattg ggaaaatata gagtgctgag agcacatata
    11401 tgaacgcagc aatatacaaa gcgatcctaa gataccaatt gtgtatgaaa gaaagatcca
    11461 caagtaaaac aatcccgatg gcgccatata tccagctcat cagcaaggtt tcacgatagt
    11521 tttctttaaa atatgtgaag tacgttttcg tcaaaggaaa cgtttctttt gagcgcagcc
    11581 attttctaat cactgcaacc atcgcatacg atgcaggacc gaaagtaaaa actcccaaac
    11641 ctgcgatgac taaaccgccc cacagaaaat tcaaataaac caactgtaaa atacgattta
    11701 gccttccgtt tattttatct aatgactgca gcatatcttt ccctctcttt ctatcttagt
    11761 aaacgccgtc ttctcctagc tttttggcaa tcttattagc taaaattacc ataaacaatc
    11821 ctacgacccc tttaaacaat cccattgctg tactgaaact taactgtcca ttcttcaatc
    11881 cagctgtata aatataggtg tcgaaaattt ctgcaacgct tcgattcaat gaattcaata
    11941 acaagtacat atgctcgaac cctaagtcta atgtctgacc gatttttaga atcagcaagg
    12001 taatgattac tggacgaatt gccggcaatg taacatgcca tgttttgcgt aagcgtcctg
    12061 ctccatccat ttctgcagct tcatacaact gtgtatcgac tgcagttatc gctgaaaggt
    12121 agatgatcgt agaccagccc aactctttcc agatcacttg tccgatatat actgtgcgtg
    12181 tccattcagg ggatgttaga aaactgattt tttcaaatcc cattgttgca aggaattcat
    12241 tcaatacccc tccgtcaaca ttcaaaaata cataagtaat cgaaacaatg attacccaag
    12301 acatgaagtg agggatgtaa atgatcgttt gaatcccgtt tttcaatcgt ttgttcgtta
    12361 cttcatttaa tagtaaagac aaaatgattg gcatcggaaa aaacacgaag atatttaatc
    12421 cgaacaagat caacgtattt cttaataatg taaagaatgt cggttctgta aacaaacgaa
    12481 tgaaatgttt gaaaccgacc caaggacttc ccattattcc taaaaatggt tgataatctt
    12541 gaaaagcaat aacgagtccc cccataggaa gatacctaaa aatcaagaaa taaaggactc
    12601 ccggcagaat catcacatat aaaaatttat tcgttttgat tcctgcaatt attttttttc
    12661 gtcttctgag cttttcttct tttttcgata gcgtcagttg aaatggttct tgtttcttta
    12721 cactttccat aagcccctcc ttagttttgt gaatgtttgc tgcttatgac ttcatgatag
    12781 ataactatct gaattacgta tataatcatt tttaccgtaa gtaaaaacgc ttacatagca
    12841 atgatttttg tgctatacaa aacgataaag tgtatttttt cacaaataaa ctacgcatag
    12901 caaaccttct atttttttct atctgacact tagcatagga aatgattata tacgtaatct
    12961 ttcaccgatg ataaattgaa catatagaaa cagaaacatt taatttattt tgaggaggag
    13021 cctatctata tgaaaaagaa agtctctata ggagaaagaa gttttacact tttcaacaat
    13081 acgtttctaa ttttgctagc tttgatctgt atcgttcctt ttttaaatat catcgctaca
    13141 tcttttgctt ctacacaaga agtcgtcgcg aaaaagttta tcctttttcc tactaccttt
    13201 tctttagacg cgtatcgcta cattctttct acgccgacca tttttagagc acttgctgtt
    13261 tcgattggtg ttacaggtgt cggaacgatc gtcagtatgt gtgcaacttc actgatggcc
    13321 tacggtcttt ccagaaagta tttgtttggt cgaggtttcg tcaactttct tgttgtattt
    13381 tctatgttgt tcagcggggg gatgattcca acgttcttag ttgttcgttc tctagggctg
    13441 gtcaattctt actgggccat gatcctgcct gtggctgtta atgcaatgaa tatgatcatt
    13501 atgagaaact ttttccaagc cttgccggat agtttggaag aatcagcaaa aatggatggt
    13561 tgcacagatt ttggtgtatt tttcaaaatt atgctgccat tagcgttacc gtctattgct
    13621 actatctcgc ttttttatgc tgttacttac tggaacactt atatgactgc tattttgtat
    13681 atcaacgatt catctaaatg gccgatacag attcttttac gccaaatcgt cattgtatca
    13741 agtgggatgc aagcagaaag tagtgccgtt gatgtgattc caccagcaca aacagttaaa
    13801 atggctgtta tcgttattgc aacagtgcct atgctgattg tttatccttt tgttcagaaa
    13861 tattttgtca aaggagcact cgtaggttct gtcaaagggt gattatgtac tgaacgcaaa
    13921 atggtagttg tgtcagaagt ggtcagcttc aagaaataag aaggcattta cgaaaattgc
    13981 ttcttaaatt tttgtgaaat ttcggcttat ttttcgaaga atctactcct ggaacaccgt
    14041 gtatcagagg ttgtgaaaga gacgttcagc cttgagcgat gataaaaact ctcacaaaca
    14101 ttgcgacgag taacccacag gagcaataag aagcgatttt gaaaaatagc ttcccatatt
    14161 tcacaaaatt atgccttatt gtcgatagcc tgtctcttga acgccattta ttcgtaaaaa
    14221 agaactgtga caagactttt gtcacagttc tttttgcgtg cttagccttg aaatttaact
    14281 atccataaat acaaatacta gggttattct aacttctttt attgcttgaa tcagtccaca
    14341 agaaaaacag cctcttgatt cacaaatatt tttttcgata ttctcccggc gtaatcgatt
    14401 ccttcttttt aaagaaacga ataaaatttt gcggatttcg atattgcaat ctttctgcaa
    14461 tctcttttac ggagaggtta gtctctttca accatttttt tgctacctct aaacgatgat
    14521 tttgaacaaa atcaccaaat ttttctcctg tttccttttt aaatacatta cttaaatagt
    14581 tagggttata atgcaaccta tcagcgatca catctaaaga cagatcacga tcatattctg
    14641 tgtagacgat atgcataata ttctccgaca aatttttaaa ttcatgatca gtgattgctt
    14701 gagagcattc tgtgattggc agaactaatt gttctaaaac aagtttttct agttctttgg
    14761 gatgataatt gttcagtgct tttacataaa tttgtttcat gttatcaaag ttttttgaat
    14821 ctactcccaa taactgtcct aattgaacga gttcattgat caagcggacc gaaactactt
    14881 cccgactcaa aggattttta ttttgtgcaa acaatgcatc aatcaattca tgtactaaat
    14941 tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa tcgattttgt tcttctatcg
    15001 gatatctaat aagtgttttt tcatgagcag caggaacgat ttcctgataa aaaataattg
    15061 aacttggacc agtatttaca cgataataca gggcttcttt tgcccgatca accgattgct
    15121 tgctttcatt cagcgtttcg aaccggttac tgatgcctat actgatagtt aaattcaagt
    15181 attcttttac acgtttttga atgagtcggc aatactccat gacttttttg cttgcttctg
    15241 catcatcttt atccattcga taaatagtgg cttgcatttc ttcattcaaa acgatcggca
    15301 gcattcgatt ttcctttggc acaatttccg tgatcatgtt gttgatcgct aaaagtagca
    15361 aatcacgctc cccaccgtga ctatcttcta acagatcgat ttgaaccaaa gctgtataat
    15421 agcagtcatt ttgagaacta taaccaaatt gctgcaaccg ctgatctagt tctcgccttt
    15481 ctactcgatt tctaaataaa ctaagtacaa atagcgtttc cagctgtggc ttttgggtaa
    15541 ctaagtgtgc acttaacgct tctttttctc ctatgatccg actgactgat tgagcgacca
    15601 aatctagttc tttcccactg aatccttttg atttaagatt cagacgttcc tgtatttgag
    15661 aaatcggacg ggcaaatctg tccgaaaata agtaggaaag aagaccaatg agtcctacca
    15721 gtgttaaaga cactgcaact agcccgattc tcagattgcg gatcattgcg ccaattgcac
    15781 tagagtcaac ttctaatctg taaatccaat tattataatc tgatttcagt actatatacg
    15841 ttttatcttt ttctcttatc actttcagct gattgtaatc tagatccacg tccgttaata
    15901 cagttggttg ctctttattt tttactgtct gagtagtaaa aaggttgcca acttcgtttt
    15961 caatagaaag tatgtcttct ccactttcac caacaacttg ttctattgta tgtttctcta
    16021 tcgtagcaat tcccaatgca tatttttctt tttcaaacat tggcaaagaa atcaccattt
    16081 cgattccttt gtttttcttt tgccaaaata aattattttt gttatttatg tacttctgtt
    16141 gatacttttc aacttcctct tcggtcagtt gactcaacga tccattgatt actccccatt
    16201 taccagccaa actgactaaa tcataattat tatcatccat aacaatcgtt tctataaaat
    16261 ttagttcttt cctgatttca gaataagtgg agtaatctgt atatgtcagc ggatgcttga
    16321 atatgtccga gaaatttttg gtcgaaccat acgtagaaaa cgaatattca atcgttctaa
    16381 ttttccattc aacgttagtt ttaacttgcc tgagcaaatt ctttttgtct gttaaataac
    16441 tcgattcgat cgattcatag gtattctggt aaatgaataa actaaatcct atgacaagta
    16501 cggctcctaa taaaaacatc ggtaaaaata ttttataaaa tactgtactt ttcttcattt
    16561 tcttcctctc ctccttttaa aataaaaggc tttcattttt ctgtaaaatt gatactcatc
    16621 taattaaatg agcattcccc gctttcaaga atagcataat taaaaataaa aatccaagta
    16681 atttttaacg tgtgcaatct tttcatatca aagatctatt gacttatcgt agttatcgta
    16741 atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa aatgatggaa tggtaacgat
    16801 caaagatatc gctaaagcag ctggcgtttc tcatacgaca gtttctcgtg ctttgaataa
    16861 cagcagttta ataaaaaatg aaacaaaaca aaaaatacga caactagcta aggaaatgaa
    16921 ttattcgcca aactatagtg caaaaggttt agtgaatcaa aaaaaatatc taatcggact
    16981 atttttctca agtttgcata aaggaacttc atcaaatttc ctagcagatt ccattgcagg
    17041 catccattca gtattagaca caaattacag tttatctgtt gaaagtatcg atgttgttga
    17101 tttaacagaa atcaacctgc aacgctacga tggactgatc gttatgagtc aaagtgatga
    17161 tgatcagcct ttcatcgact atctcaaaga aaaagaattt cctttcgttg ttgtcaatcg
    17221 tcacatcaat gatccagcgg ttatcaacgt cactgccgat gatgcggccg gtgttatgca
    17281 ggcagtagat tacggtattg gatgcggaca tacgaagatc gcttatattg gcggaaagaa
    17341 aaattttcgt tcgacaaaag aacgggaaaa aggggtattg aaaagtctaa aaaaagcgag
    17401 catccctgtc aatccagcct attttttaac aggagattac agcttagaaa gcggcctcca
    17461 aaacatggaa tacctattat cactttctga cctgcctact ctggtttttt gcggcaacga
    17521 cgacatagcg attggtgcat tgagagctgt acatttagct ggcctccgtg ttccccagaa
    17581 catttctctg atcgggttcg atgattctcc agtcgtctcc tatctcaatc cgccgcttac
    17641 caccgtacgt aagcctttga aagaaatgtg tcaagaagga acaaagcttt tactagattt
    17701 gatcgaaggc aggacgatcg aacaatctaa aattgagctc tccaccttgt tgatccttcg
    17761 tgagtcgatc aattacttgt aaactacaag taattttata taaaaatgtt aacgtgtgca
    17821 taacacagaa aggaagttta tatgaaaaaa ctgacaaaag aattagtgaa aaaaaacgaa
    17881 gcacctgtaa aagttcttca gtttggtgaa ggcaatttta tgcgtggatt tattgattgg
    17941 cagattcagc agctgaataa ccaacaattg tttaaaggta atgttgcaat cattcaacct
    18001 ttagaacaag gtcttggaaa tatgatgaac cagcaagata atttatatac ggtcatttta
    18061 caaggactca gcaatggcga aatcgttgat acttccgaga ttatcaccgt agtagaacaa
    18121 atgatcaatc cttatgatga atgggatgct tatctatctc tagcagaaaa tgacgacttg
    18181 gcctatatcg tctccaatac aacagaggca ggaatccaat tcgtggagac tgataattta
    18241 acagatactc ccccacagag cttcccgggc aaactgacag cctttttata ccgacgtttc
    18301 aaattaaaca aagctggctt taccatcatt ccttgcgaac tgattgacag aaacggcgaa
    18361 cgtctaaaag aaatcatttt acgctacgca gaaaaatggg atttagaacc tgcttttgtt
    18421 gagtggttag aaaaagaaaa tgttttctgt tgtagtttag ttgatcgcat cgtcccaggt
    18481 tatcctcgcg aagatgcagg tgttttcaat caacggcata actatgaaga tcaattgatg
    18541 gtcaaagcag aaccattcat gctttgggtg atcgaagggc ctaaagaatt ggagaatcaa
    18601 ctaccattca aacaggctgg cttgaatgtc atcatcacag atgatatgac tccttatcgt
    18661 gaacgtaagg tgcatttgct taatggaccg catacagcga tggtccctct tgctttgttg
    18721 gcggaattgg aaactgtaga agaagtaatg aaagatcctt tattctccgc atatgttgat
    18781 cagttattca atctagaatt gattcctatg ctttctttac ctaaagatga attagccatt
    18841 tatgctgacc aaatcaaaga acgtttcttg aatccttttg cccatcacaa attagaagcg
    18901 atttcattaa atagtgtttc aaagttcagc acccgtctgc ttccagtgtt caagaagtat
    18961 atcgaagaac aaaatcaggt accaccgtta atcactgttt cactagctgc gctacttctt
    19021 atgtatcgag gcgatcaagt aaagccacat gatgatgaaa agacgataag cgaatttact
    19081 gatgcttggt ctgataatgg aacagctatt cctcgattat tgcaaaacgc tgcgctttgg
    19141 ggagaagatt tgtctcaaat accgaatgtg acagatacag ttcaagaaat agctgcaaag
    19201 atcgatcaaa aaggtgtccg gtcagtatta caagaaatga aaggaacgat ggtccatgct
    19261 tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac agtgtggtcg tcgcattaac
    19321 ccctattcca aaacaaacac aactgacgat tgatcagcaa acgatcacca ctcttgaaga
    19381 tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa gctggagaca acgtgatcaa
    19441 atatggctat ccaatcggtc atgtgacgac agacgtaact gccggtcaat ggctgcacac
    19501 tcataatgta aaaaccaatc tttctggtga attggattat cgatatgaaa aagatgtcca
    19561 tccaagtcat tacctttttg agaaccgaac atttcaaggt tacttgcgaa aaaatggaaa
    19621 agtagggatt cgtaatgatt tgtttttagt acctaccgtt gggtgtgtga atggcattgc
    19681 tgaactgatc gtcaaacagt tcaaggaaaa acatccagat ctgggacagt ttgaccatat
    19741 tacgatttta aagcatcctt atggctgttc gcagcttgga aaagatcatc aaaatactcg
    19801 agaaatttta gcggacgcag taaatcatcc caatgccgga ggagttttag tatttggatt
    19861 aggttgtgag aacaatacag ttcctgaatt caaaaaaatt ttaggtgaat atgatgaaga
    19921 acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag atcgaacaag gtgtggctct
    19981 attagaagag cttttaacag cggcggaaaa tgaccagcgc atatcagtcc cgctctctaa
    20041 gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta tcgggaataa ctgctaatcc
    20101 tttacttggt gcattttctg actacttgat tgctcaagga ggcagcacga tcttaacaga
    20161 agtgccagaa atgtttggcg cagaacaagt attgatggca cgagcggaaa atgaagaagt
    20221 ttttgactca atcgttgact tgatcaatga cttcaaacag tatttcttgt cttacgggga
    20281 accagtttac gaaaacccat ctcctggtaa taaagcaggt gggatcacta cattagaaga
    20341 taagtcttta ggctgcacgc aaaaatcagg aagctctcct gtcgttgatg tgctacaata
    20401 tggcgaaaaa atcagaaaac cagggcttag tttattgcaa gctcccggga atgatcttgt
    20461 agctgcctct gctcttgctt catccgattg ccagttggtc ttgtttacca caggaagagg
    20521 gactccgttt ggctcatatg tcccaacggt caaggtatct acgaatacaa ctctttttga
    20581 acgcaaaggt cattggatgg attttaatgc aggcgtctta ttagaacaac caatggaagt
    20641 aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct agtggagaag aaacgaaaaa
    20701 tgaacaaaat gacgttcgag aaattgccat ttttaaaaac ggagtaacac tttgattttc
    20761 ataaaacagt ataaaaagaa agaaggaaga tttatgtttt tatctgatga ttttttatta
    20821 aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa aaaacatgcc cattattgat
    20881 tatcactgtc acttagatcc aaaagaaatt tacgaaaaca acaatttcag caatctgaca
    20941 gaagcttggt taggaggcga tcattacaaa tggcgcttga tgcgagcttg cggcgtgcct
    21001 gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat ttttagcttg gtgtcaaacc
    21061 gtaccaaaaa ttatcggaaa tccgctttac agttggtcac atcttgaatt gaagcgtttc
    21121 ttcggtattg acttgttgat aaatgaagaa aatgcagaga aaatttggac acaagcaaat
    21181 gaaattttgg cagctcctga ttttcgccgt cgggaaatcg caaaaaaatc gaatgtagaa
    21241 gtgatttgta caacagatga tcccgcagac gacttgcact atcatcagtt gcttgctcat
    21301 gaagaaccag atttgaaggt tctaccttca ttccgtccag ataaagcact caatattgaa
    21361 aaagaaggat ttaatgcatg ggtcacacta ctggaagaag cagctgacca aacgatcacc
    21421 acctatcaag aactgataga agtcttaggg cagcggatag aatacttcca tcaacatgga
    21481 tgtcgtgttt ccgaccacgc tttagacatc ttacgctata aagaagcgga tgaaacagta
    21541 ttagaagaga tttttcaaaa agcacgagct aacaaactat tatcttcaga tgaaatcgat
    21601 gcttatcgta cagaaacact gatacatttg acccacttct atcattctca taattggacg
    21661 atgcagctgc acatccatgc ttatcgtaac tgtaatacac aaatgttgga aaaattaggg
    21721 cccgataccg gctatgatgg aatgaatgat ctttctttga ctattcctct gcaaaaatta
    21781 ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga tcctttattc attgaatccg
    21841 aatgactatc ctgcgatttt agctttgatg ggctgtttcc aaaaagaaca gaatggaaaa
    21901 ctgcagttag gttctggatg gtggtacaac gatactcgag ctggtatgcg cgatcaattg
    21961 acacaatttg ctgatggtag tgctttgggg aattttgtcg gcatgctgac agattcaaga
    22021 agtttcctgt cctacactag acatgaatat ttccgtcgtg ttctttgtga atttttaggc
    22081 gaaatggtca tgcgaggaga agcacctgaa gatgaacaac ttttaggaag tctagttcaa
    22141 gcgattagtt atacaaatgc taaaaattac tttggctttt ttgcctaatt gattaatagt
    22201 aacagtaacg aatcaaaaac aggatagtga aataacggaa tccgtttatc cacgttttag
    22261 agaagaagac ggaccaaatt tgctccgtct tcttctcttg ctgttctgct tcattgccta
    22321 taaaccttta tgaaagaaga gataacatgc ctactatcca tattgccggt gattctactg
    22381 cggcagaaaa agatgaaaac aagcgtcccg agacaggttg gggagaaaaa atctctgctt
    22441 atttttcacc cgagatccag atcaataacc aagcaaagaa tggacgcagc accaaatcat
    22501 ttatccagga aggaaggtta gcccgtctag cttcacagtt tcagccgaat gatttcttat
    22561 ttgtacaatt tggacataac gaccagaaaa tccaagaaga aagaggtacg cttcccttcg
    22621 gtagctattt aaaaaatctg cagatatttt ttgaatgcgc aaaaaaagcg aacgtccaaa
    22681 tggtcttact gacatctgtg acgcgacgag attacttaga aaacggaaca ttgaatccag
    22741 atatacttgg agactatcca aaagcgatgc gtgcatttgc tgagaaacac catattcctc
    22801 ttttagatgt gttctcaaga tcacaagaat tgttccagac cttctcaaaa gaagagacaa
    22861 aaaaattcta cctgcatctt atacctgata cttgtaaaaa ttacccagaa ggactaaaag
    22921 acaataccca ctttagtcca gaaggcgctg ataaagttgc ccgaataatt gttgaattga
    22981 taaaagaatc ccgtctgcct cttgctaatt atttacaaaa aggagtttga gatgtttcca
    23041 ttaaatttag gcatccgtgc ccacgattta gatgttcgaa atcgtgaaga aatcgttcaa
    23101 aaagtatcac agcttcactt gtcccatatc caatttgcac cacaaaaagc atttcctgaa
    23161 caacttaaat taactgacat gacacttggg agtgctgcct attttggtga atacttccaa
    23221 aaaaacaata tcgaactttc cattttagga tgctatatca acctatccag caaaaatcaa
    23281 gaagttcgtg cacaagcatt ggcaactttc aaacagcaaa ttgctctgtg ctctgcctat
    23341 caagccaaga tgattgctac ggaaaccggt agtgtctctg tcggttacac aaaagaaaat
    23401 tttactgaag aagcttatca gatcgccaga aattctatca ttgaattagt cgctcatgca
    23461 gaaaattttg gcgtaactgt tgctgttgaa gcagggatca atcatccaat ctataactat
    23521 cagttagcta aacgtttaat cgatgaagta gcttcaccaa atttaaaaat cattttagat
    23581 tgtgcgaatt tgatgcataa agaaaatcat gctgatcagg aaaaagtgat ccgaggtgcc
    23641 ttggacaatt tacatggtta tattacagcg cttcatctca aagactatat catggaaaat
    23701 ggaaagatcc gcatggtacc tgttggcaag ggctgtttag atttccgccc ggtactgcat
    23761 tatatcaaat atgagcgtcc tctaatgtac gccactcttg aagctactcc tgagatttat
    23821 gttcctgaag caatagaaca cttgcagttt atttaccaag attgttaagg agaagattat
    23881 ggaggaacga atacagaaaa gttatcaaaa atatcatgca aatgcttttc agacattgct
    23941 gcatctttat cagggaaatt attggcgttt tgccatgtca ggactctttt ttattatcaa
    24001 acatcttcca tcttggttaa tgccgatcgc tattgccaat gtaatcaatg cagcttcatc
    24061 gaaagattct tctcagttgc aggccattta tttcaacgct tttttgatgg cttgtctgat
    24121 tggtcaaaat atattgacga attattttca tgtaaaatac catagcactt cgatccgtca
    24181 agtagaagct ggactaagag cgacccttat caagaaaatc caagaactat ctattcctta
    24241 tcaaaaagaa ttgcaatctg gacgaatcca gtcaaagatc atccgtgatg tagaagctat
    24301 ccaaacgttg tcctctcaag tatttgtcag cggattaaat atcgctgtca atttattcgt
    24361 agctttaggt atcacgcttt ataaaagtcc tgttgttttc atgtttttct tattgactgt
    24421 tcctctcgcc gcattcgtta ctctcttttt ccgtaaaagg atcagacagt ccaactcaga
    24481 cttcaggcaa gaaatggaag agacttctgc aaaagtgatc gaagtgatcg aattagcacc
    24541 gattgctcga gctcatgggt tagaaaaaca cgaaacaact cgcttaaata agcattttac
    24601 tcaaatctat gaaaaaggat tccacttaga tatcctgcaa tctgttttcg gatcagtcag
    24661 ctgggctagt tttcagatat ttcagctgat ttgtttgatt ttcactggga tattagcttt
    24721 acaagggcgc atccaacccg gcgatgtcgt gatgtatcag acgtatttca cgacgatcgt
    24781 caattccgtt tctggaatga tcacgctgat tcctacgatt tcaaaaggca tggaatctat
    24841 cacttctatt ggcgaactgt tgattgctgg cgatgtagaa acttatcacg gtaagaaaaa
    24901 aatccaacat ttggatggaa attttcgttt tgaggatgtt tcttttcttt atccggactc
    24961 actcaaaccg attatcgatc atttttctct agaggtgaaa gctggagaaa caattgcttt
    25021 tgttgggcca tctggctctg gaaaatctac actccttaac ttattgattg gatttatcca
    25081 gccgacttca ggaaaaattt ttctagatga tcagcctttg gatgaactgg atatgcgaag
    25141 tgtgcgaaat tatttagccg ttgttcctca aaccactttg ttgttctccg cttcgatcaa
    25201 agagaacatc acttatggct tgaaaaacgt atcaaaagaa cgtttagatg aagtcatcga
    25261 agctgcgcaa ctttcttcac tgattgacca gcttcctgat ggtctggata ccttagtagg
    25321 cgaacatggc aacaagctgt ctggtggcca aaagcaacgt atctccattg cacgagcgtt
    25381 gatacgtcag ccaaaaatca ttttgctaga tgaagcaacc tcagctttag ataaccagtc
    25441 agagaaaaaa atccagcaag ccctgaatta cctgacagaa acacccacta cttttattgt
    25501 ggctcatcgg ctttcaacga tcaaagaggc agataaaatc gttgtgatcg atgaaggaaa
    25561 aatcgttgaa ataggtactt acgaaaaact gttagctcaa aaaggctatt tctaccgctt
    25621 gaaaaatgaa tgacaaaaaa taggagctat gacaagactt ttgtcgtagc tcctatttct
    25681 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatagtc atcaaacatg
    25741 agaagctgtt ttttgaaatt gttccttgta actcagagtt aaacggtagg ctcacgacct
    25801 cttggtaaat ggtgttcaag agacagccta tcggcaataa gaagcaattt tgtcatcgct
    25861 caaggccgaa cgtctctttc acaccctctg ttgtttcata aaatagtttt attcgctgta
    25921 aaggtcgcga actggtgtca tgatgatacg gttcaagtca ttgatgattg tactgaagcc
    25981 ttgctctttt tgcatcaagt cattgattac ttcttctgat tggattttca cagccatttc
    26041 ttgtgctttc attgcatctt cgtcgctgaa ctcttgtcca ctcatttgtt tttcttgtaa
    26101 ttcgatttgc atcgcttgga agtctttgaa cagtttgtgt gcttcttcgt ttgcttgtac
    26161 tttctcgtac gcttctttca acgctttgaa ttctggtaat tcacggatct cacgttcgat
    26221 ttgattcgcg ctatcgtaga tgtttgacat gttatgccct cttttctttt agtagttact
    26281 ctctattgta acatggggtt attcgttttc aaaggggcta ctctccttga tatttccgcc
    26341 agaggtcttt agccgcttca ccaattccct gaacgccttc tttcaccata tcgccgaagt
    26401 ttttcagccc ttcttttgct tgttcaccat attgattcaa gttatcttgc caatttccag
    26461 actctccatt agaatttcct gtgtttccaa cttgatctgc agctaccact tgtcctcctg
    26521 tggcataggc atctgctaca ctaaatcctg tttttgaagt atacggaagg atgccttctg
    26581 ccgctgattt gaagactttc ccgacaacat ttccacttgt cccttccaaa tagtgcagct
    26641 tgctggattc ttcgtaaccc atccatgtcg aaatcactac gtctggcgtg taaccgatca
    26701 tccattggtc atttgctttt gtggcatcga aattagtttc tgtggttcct gttttccctg
    26761 ctatcgtata acctgcagga ttcgcagcag ccgctgttcc atttgaaaat gtaccaagaa
    26821 gcatgctggt catttcatcg gctgtttcct ttgatatgac ctgttttggt tttttgtttg
    26881 tgttatcgac gatcaccgct ccagtcgaat caacgatttt cgtgataaga tgcggcgtat
    26941 acatctgacc atcattggca aatgcactgt aagcacccgc catgatcaat ggagatactc
    27001 ctttttccag tccaccaagg gctaagccat aatacttatc agattttgct aaaggcaggc
    27061 cgaattcttc tgctttatca tatccttttt gtaaaccgat ctcatgcaaa agccatacag
    27121 taggcaaatt caaactttgt gcaacagctt gatacatcgg tacttctcct tggtatgtcc
    27181 cgtcaaaatt atgcgcatcg taataacttt gcggttcgtc tttcaagata ctggaaggct
    27241 tgtatccagc ttccagtgct ggtgtatata cgcttaatgg tttgatcgtt gatcctggtg
    27301 aacgacgcat ctgtgtagca aagctgaatc ctctaaagac atgatctcct cttcgtccta
    27361 ctagtgcttg tacaccacct gtattcggat ctaaagctac tgaagcagat tggaccatgg
    27421 caccatctga agcatttgct gggaataatg cactgttttg ataagtagct tctaattgct
    27481 tttgataatc ctgatctaaa gctgtataga ttttatagcc tttattcatg acatcttctt
    27541 catcaagacc atactcgtta actgcttcat cgatcactgc atcaaaataa taaggataac
    27601 gataaccaga atctgaatta tcgtatgtgt cattaagata cgcgctgata tctgtctggc
    27661 tttctgcttg ttcttgttct tttgtgattt ttccgttttc ttccattaca cttagtactg
    27721 tgtttttccg atcattcgca ttttttgggt gatcgatcgg attataaata ccaggacctt
    27781 tcagcattcc tgccaaagta gcagcctcag acagcgtaac attcgttgca tctactccaa
    27841 aatactttct ggaagcgtct tggacacccc ataccccgtt gccaaaatag gcattgttaa
    27901 gatacatggt caagatttct tctttgctgt acttcttttc gatctcgatt gccaaaaata
    27961 attccttcgc ttttcgatca aacgtttggt ctaatgtcag atacgcattt ttggcaagct
    28021 gctgagtgat cgtacttcct ccgccccctg atgttccgcc agacaagacc attcggactg
    28081 ctgcacgggc aatccctttg atatcaaacc cgtgatgctc gtaaaaattc ctatcttctg
    28141 tcgagattac tgcatcttgg atatagggag aaatcgcgtt taattctaca tatgttcctt
    28201 tttgggcgta cagtgtgcca gcttcttcgt ttttcttatc ataaatgact gtcgattggc
    28261 ttaatcctga ttttaacgtt tcgacatttg cttgttttgc taaaataaag aggtagatac
    28321 ttgtcactaa gacacagatc aaaccaatca gcaaaatcaa tttgttcaca tgatattttt
    28381 tccagatgcg ctttcgccat cgatggaatt gtccgagata tggtctgatc caacgccaaa
    28441 aactgatcaa agcagctttt atcttttcta aaatgctttg aaaatccatt tgcttcttct
    28501 cctgtctatt tgattcaatc gtcttatttt accatagtga aagagtgttt tcttcctgct
    28561 taagttgtag ttcaatatgt ttttttacgt tagtgcaagt agagtaacat aagaatgaac
    28621 agaaaaatgt aagttttttc ttaaatatgt tttaaaaatg gtacaattag tctgcgattg
    28681 cactgaaagg agtacacttg tgaaaatcac gatcacttta cctatgaacc atcctgaaac
    28741 aacaattcgc gacttgcttg aaaaggaatg gttagttcct agaaaggttc gacacttttt
    28801 gcgtatacgt aaaaacgttt ggatcaatca acaacctgct ttgttccatc atgaagtaaa
    28861 gggcaatgac cagatcacat tgttattcga agaaacagat tataattatc aagaagttca
    28921 attaggagat gcgaagcaca tccgtgtttt atatgaagat gagcatttga ttatagtgga
    28981 taagcctgca ggaatgaaga ctcatccaaa tgaaccgaca gaaaacagta ccttactcaa
    29041 tcatttagct gcttatctcg acactaaaca gcaacgacct tacgttgtcc accgcttgga
    29101 caaagaaaca agcggtgccg ttttatttgc aaaaaatcca tttgtcttgc cgatacttgg
    29161 aagaatgttg gaacaaaaaa tgatctatcg gagatatcaa gccatcgtat ggggagaaat
    29221 tgccaaagat atgaccatca ccaataaaat tggacgagat cgccatgatc ggagaaaaag
    29281 ggtcgtagat gataaaaatg gtcagagcgc cttgacacat gtcactgtca acaaagtact
    29341 ttctgaacaa acacaagtct attgtgtttt ggaaaccgga cgtacacacc agatcagagt
    29401 tcatttagct cataagggac atccgatcgt tggagaccct ctctaccaaa atagaacagc
    29461 tgaacgactg atgctccatg cattagaact gcacatgatc catccattta ctcaagaaaa
    29521 aatagtcggt aaagctttac ctggattatg ggaatagaaa taaaaaagaa cagagtctta
    29581 tcagcagata atgataagac tctgttcttt ttgtgtaatt tgtctgctta cttttgatgt
    29641 tcgtgttctg ctaacagcaa actaccgata atcccacttt cattcggcag tccccaacga
    29701 acgatatatt cacttacttg aggtgtttcc atataaccac ccatcaattc aacaaattgc
    29761 tgacggattt tttgaaggag atgatcctga ttcattaccc caccgcctaa aataatcttt
    29821 tcaggtgcta acgtcaacgt atagttgatc agtgcttgag caatataata agcctgaata
    29881 gcccaaacag gatgatcttg cggcagatct tgccctttga tccctgtgcg tgcctcaata
    29941 gacggtcctg ctgctaatcc ttctaaacaa tctttatgat aaggacaagt accttcataa
    30001 tggtcatctg gatggcgttt cacccaaata tgtcccattt caggatgtgc gatccctgaa
    30061 aaaatttctt cattgatcac gacacctgca ccgattcctg ttcccacggt aaggtaaata
    30121 caactatttt ttccttgtgc tgctccttgc ttcaattccc catatgcagc agcattgaca
    30181 tccgttgtcc aagcagcagg aatattgtag cgttccttga ttgtgccaag aaaatcgaat
    30241 tgtgcccacc cttttttggg agttgccaaa acgtatccgt aattatcttt tgttttgtcg
    30301 ataccaatcg ggccgaatga gccaatcccg attcccttca attcataacg atcaaaaaat
    30361 tcaaatacct gatccaatgt ttcttctggt gttgtggtag ggatacttaa tttttctaaa
    30421 atttctccac catctgaaac cgcacacaca aattttgttc cgcctgcttc tattccgcca
    30481 tacattcgtt ttcctcctcg aatccgattg tccgaatctc ataaggttgg attattggga
    30541 caaccgcttg cttcgttggt tcttcaagca gattcaatat gacagttttt ccattgtctt
    30601 tagttatttc caatttttct aaatgactag acatattgaa acctcttact actactttat
    30661 cactaaactt gctgcgtttc aaagctgtaa ttgcaaacgt ttcacttttt atcgttaaat
    30721 attgttgttt tgaaatcaat gttccttcat gatgtttgat ttgttgagtg gaaaaaggaa
    30781 tctgtgctgc gtatgcatgc aaataagtag agaatttttc ttctggtcca tgtagttcaa
    30841 tgctgtaatt gaaccgatgc tcacctagac actgtgcttc tggtgttgga aaatatcccc
    30901 aatcgcctag ttctcccaca ctgcgaagta gcgtcactgc gatttgtccg tcttctgtta
    30961 cttcatattc attcaaacca aagtttccga ctgttacacc gtactcttca tcatgaatat
    31021 tgacaaatgc ttgctgatgt tgcggattcg tggggttttc ccaactttta gacacatggt
    31081 ttgggcgctt caccacttca aaaatactat ctgcttcgtg atgttccaca tgtaactttg
    31141 taggaaacaa gacacgtaag cgatgatctt tcatctgatt gtcgagagtc gtttcaaagt
    31201 cgattttttt actatctttt ctgattgtca tgattgtttt tatttgaagg attcgttttt
    31261 ctttggatcg ttctgctttt cgataacgaa attccacaac catctgctgt tctttttcta
    31321 aacgctcgtc tgcagaaaca gggatttcca tttcctgtag aatacttatt tttgctattt
    31381 ccggtgtatc tactaatact tttacttcag agttttcttt gttactggat aggattggct
    31441 gatcacagaa aggttgttta aagatatact catttcctat atcgccgata tcttcaaaaa
    31501 tatgcagatt attcaacttt cgtttgtttt tgcgatcttc cattgtaatg gtaccatttt
    31561 tttcaatcgt caaatgtaga gggccgtttt cgatgattcg tccactctga tgaatcattg
    31621 tttccttttc ttctgctttc gcttcccctt ctacaagcgc gaatgtttcc caagaaaatg
    31681 ctggcatgtt ttctaatggt aaagtcactt tgatcattct tgccatatat ggcacacgga
    31741 aacggtcttt cggcaaatca taaccaaatt ggacagtttc ttcagataaa atagctggta
    31801 tagtggcccc actttttgtt tctacatgat acttacgttt aggcaaattt tctaactctt
    31861 gatatagttg ttcaggtatt ccttcagcga atctttttct ttctaattcg atggttattt
    31921 ctgcttcacc agttttatcc attcctgcag tatttacaat gacaaatgga aagctgtctt
    31981 ttgggaatcc ggtcgtatcg attgctgcgg tcagctgttc taaggaatct tgtgcaagat
    32041 actttccaac ttcatctgct ttttcaaaac gaggtatcat ttcccgatgg acagaatcga
    32101 ctgaacaacc gcaaatactg tcgtgaggat gattttgcat taatgtcttc caagcataat
    32161 ctaattgatc atggggatag tttccactaa cttcataagc catcgtcgca agtggttctg
    32221 taatattttc tagctgtcgc tggaccttgg tattccattg ttttaagtaa acccgagcag
    32281 atgctgtatt ggctaaagta taccagccat ccgtttcttg acttgtcaat tctccttcta
    32341 ctgagcctaa gtcttctggc acatcttttt gaactgcttc cagatagtca gtaaaattag
    32401 aatgaataaa ctcgtaatct ggaaacagtt catttgcgag atggattgct tttgatatat
    32461 ctttttgtac cggttgatga tcaacaccat tcatcatcaa taagtggttc gtagaggcat
    32521 attgttctgc atccgctaat ttttgcttcc aaaaggcaag agctgcttct ttttctactg
    32581 gaatctcatt cccgttgcta taccaattgg caaatagcag accaaagatt gctgtttgat
    32641 caggtccttt ccaccacatc tctgagtatt gggaagaata gttttctgct tccaaaactt
    32701 gattatcaaa gccaattggt tttacaccac ggccaaacgc tgctgctgaa ataccagctt
    32761 gtttcattaa ttgaggcgtc tgtcccatat ttccaaatgt atcgggaaaa tagcctaaca
    32821 tgacaggggt tccccattta cggctttctt ccatgccaat cagcatattt ctcacgttcg
    32881 attccgagct gataagaaaa tcatcttgca agatataaaa tggtccaatt ctcaattttc
    32941 cttcattgat tgcttgttgg attgcctgtc ttttttctgg ccttacttga agatagtcat
    33001 ctaaaataat cgtttgccca tccaaatgaa aactgttgaa ggacggatca acttcaaata
    33061 gctctagtag atcgtctatc aattcaatca gtcgcatgtg atgctgttcg taagccatgt
    33121 accattcacg atcccagtgg ctatgagaaa tgatatatac tttctttttc atttacgata
    33181 cctcctactt ttctactcta atatcaaagt aatccatgac taattcgcaa aacatcatat
    33241 ttgcccaaga aaaccattct ctcgtataat tttcaggatt gttcacatcg aatccttcgt
    33301 gcataaggtg tgttcctgcg tcagttgcta caagtaaatt caatatccgc tctttttcag
    33361 atttgtctga cgttgtcatt ccttccatag ctaatgcaat cggccaaacg tatttttctg
    33421 gcgtatggga ggatccaatt ccttttgcgt actttccttc atagaaatat ggattttctt
    33481 tactcaacaa tgttcttctt gtttgcagat aatcttcctc ttgttcagaa caatatccca
    33541 agtaaggtgc ggcgatcaga ttgggcacat tactgtcatc catgactgtg gcgtttccca
    33601 ttccatctac ttcatatgca taaatcgttt gccctttttg attaaccgtt cgtccgtgat
    33661 tctgaatccc ctcttggatc tctttcttca atttctgtgt ttgctgtaca atttctttat
    33721 catcgagtat cgtttgaaaa atttcttcca cataacctaa cacgaccacc gcaaacatat
    33781 tggatggtac cagatatcca taacggcaag tatcatcgct aggacggaaa ccagaccatg
    33841 tcattcctgt aaaagctgtt tttgcacctc gcccgtcatt gatcagcgta tcttcttttc
    33901 ttgtcgtgtc tcgtacaaat gtataaggag agtgttcatg atcttgctct gttttgaaca
    33961 cgtccaagat ttttttgata ccttcaataa aagattgatt gaattgatca gtttttcctg
    34021 tatttttgta aagaagatag gccaactgta ttgggtagca taaagaatca atttcatatt
    34081 ttctttccca gatccaatcg ttcatttttg tatgatctgt ttggtggcca gcaccgttag
    34141 cttcttcgtt gaaggcattc gcatatggat cgatattgat ataaaaaaat tgctttttga
    34201 ctaacccgct gatcatgtca gctaattcct catcttcttt tgcaatcact aaataaggac
    34261 gaacttgtgc agtagagtct cttaaccaca tcgctggaat gtctcccgtc aataaaaagg
    34321 tggttccgtc attttgcttt ttgactgttg tcaacagagt attggcaaat gctgctgtaa
    34381 aattctttgc ccagtccgca tggatatccc cacatttttg tgtgatatct tccataaatt
    34441 tctgcaccga ttttggtata tctttataag ccactaataa aaccttcttt ctatttgata
    34501 tatctttaaa gattatactt catacagcca attatgtcta cgctttcaaa aaatatcttt
    34561 ttatttactt tttcatccat tatggtatta tttgaatatg atatatcatt aagaggtgtt
    34621 tatatgaatc aaccattgta ccagacaata tttaatgatt tgcaaacggc tattttaact
    34681 ggtgaattac ctgtagacag ccaactacca actgaaaaag aactttcacg tacctataaa
    34741 gtaagcagaa tcacttccaa acgtgcattg actgaattag agcaattagg acttatttat
    34801 cgcgttcgcg ggaaaggcag tttcgttaaa tccgcttctc cggaacagat tcttgcttct
    34861 cctacaaaaa cacgacggat tctgtttctt cttccatatg tcgctgattt gtctgttggt
    34921 gattttagca agggattgaa ccctgtgatg caagataaac agattgatgt catgatgacg
    34981 acgcttgact ttctacaaaa taaaacagca aaagagctaa tcaatgaatt tgacggattg
    35041 atttattatg ctcttgatac agaacaacat cttgacttac tttttgagct ttcattgaaa
    35101 gaattccctg tcgtcatatt agataaaaaa atctatgagt taccttttcc taccgttctt
    35161 tccgataatt tccaaggcgg tgcgcttgct gcctcttatt tgatcgaaca gggacattca
    35221 aaaatcgctt atttgtttgg tacgcagact catccacaat ccgttcgaca gcgttacctt
    35281 ggttaccttg aagcattgga caaagctggt ttgactttcc acacttcact ggatgacaaa
    35341 agcgcgacat tagatgaatt gctttcttat atcactaagt atgaagtaac tgcttttgtt
    35401 tgtgaaaacg atgttgtcgc tatccagacc atgaatctct tgagacagca tggttatcat
    35461 atccctgaag atttttcaat tatcggattt gatgatatcc aagcagcagc tcttgttgac
    35521 ccgccactga caaccatcgc acaagatttt gaacagttag gtaagcttgc gggagatgct
    35581 ttggtcactt ggttagagac aaaagatcga ccatccgata gtaaatttcc agtttcattc
    35641 atcaaaagac aatcaacaaa ggagaaataa aatgaatatc ggtcaaatag atactagaca
    35701 tgcaacagcg aatcagccaa atttctctaa tgggaactgc cagccttata ctggcgttcc
    35761 ttttgggatg aattattttg caccgcaaac tacggatcaa aacggcagct ggtggtttca
    35821 tcctgatgac cgcgtttttc aaggctaccg tcttacccat caaccaagtc cttggatggg
    35881 tgatttcagt catatgctgt ttacgccgat caatggaaaa ctacaggaga acaccctttt
    35941 ccatgctcaa agttcttacc gcccagaaga aagtatattt tgcccgactc atctgtcgat
    36001 ctgtcaatta cgttatggca tctgttctac attgatacct agcatgtatg gcggaatctt
    36061 atctattgat tacagcaaaa atgacagcgg tttactttta tcctttccgg gacgccatca
    36121 gctctttgtt atagatcctt acacagtgga aggaaaaatc acacattttg ctggttcaga
    36181 agatcctgat ttttcttttt attttatatt gagatttgaa tatcctttaa caattgatga
    36241 gttagctgtt tccaacaaaa atagcgactc gatcccgttg tattttgaac agacaaaaaa
    36301 acaaactatc cgatttggga catcttttat cagctcggaa caagcagctt ataacctgga
    36361 actagaacaa aaccaaagca aagagactta ccttgctcac agtcaagaac aatggcagtc
    36421 ctattttgat cgtatacagc tcacacacca tgatcaacaa caattgcgga cgttttatca
    36481 taatttatat cgggtgtttt tatttccaca aacattttat gaaattgatc ctagtgggca
    36541 aaaaatccac tatgatacga ctagtcgatc tataaaacct ggtgttctct atacaaataa
    36601 tgggttttgg gatacgtata aaacggttta tcctctttat tctttgattg ctgtagaaaa
    36661 atacgaagaa atgctggaag gttttttgaa cagctataga gaaagtggct atttgccaaa
    36721 atggctgtct cctgatgaac gtggacttat gcctgggaca ttgatcgatg cggtcattgc
    36781 agatgctgct gtaaaagata tccgacgaga cttgatgcct gaatttctaa cggcaatgga
    36841 aaaaggtgct acggttcaaa gcaaacatcc aaattatgga cgccaaggaa cagaagatta
    36901 cttgacatat ggctatgttc ctaatcacta ccacgaatca gtcaatcaca cattagacta
    36961 tgcttatagc gatttttgta ttagccaagt tgccaaggta ctagaagaag aggaaatgac
    37021 gaagaaatat cagcagcaat cattgaacta tcaaaatatc tttgactatg agtcaggatt
    37081 tatgcgttca aaagataaag atggcaagtt cagagaacca ttttcagcta atcgttgggg
    37141 acaagattat gcagaaggaa gtgcttggca aagcagcttt gctgtttatc acgatttttc
    37201 aggactgatc ggaaaatatg gtggcagtga gagatataaa gaaaaactca ttgagctgtg
    37261 caatcagccc ccaacgtttg acccaggagg atatggtttt gagatccacg aaatgagtga
    37321 gatggccgcg atagaatttg gacaattagc tatttcaaac cagccaagtt ttcactatcc
    37381 ttacttgttc agttatatag gacaacctga aatggcacag ccactgctta aacagttgat
    37441 gactcagacc ttcgacgatt ctccaatggg ttaccctgga gatgaggaca atggaagtat
    37501 ggccgcttgg tatatcttca atagtctggg attttatcct gtcacgcctg gaaccggtga
    37561 atatgttatc ggtatgccat taattgacaa ggctgttttg cttttgtcaa atggaaagga
    37621 attgacgatt gaagcaacgc ctaatcggcc gcagcaacaa tttatccata aaatcaaacg
    37681 gatggacaaa gaatatacga aattatattt tacccatcaa gatttgctga aaggcggcac
    37741 gatctcttac caattaggga tcatccctca ccctaagaaa tatcaagcaa aagatttgcc
    37801 tttttcctta tcaaactata attaacaaga ggttgtgaaa aagctgtcct gcatcaagta
    37861 ataagaacag ccaagcaaaa atagatcctc atatttttcg tttggttggt cttattaccg
    37921 cagatgcaag cttttgaaca ccgtttattg agaggttgtg aaatcagcgt tctgacctga
    37981 gtattagaga gacaaatggc aaaatagttc ctcatatttt gccatttttt gagctaatac
    38041 cgagggtcag ctgattgaac accgtttagt ggagaggtcg tgaaaaagct g
//
LOCUS       DASJST010000007        35713 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJST010000007 DASJST010000000
VERSION     DASJST010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.007"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..1024
                     /locus_tag="U4B05_000275"
     CDS             59..1024
                     /locus_tag="U4B05_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2049441.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1081..1311)
                     /locus_tag="U4B05_000276"
     CDS             complement(1081..1311)
                     /locus_tag="U4B05_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN2049442.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1397..2284)
                     /locus_tag="U4B05_000277"
     CDS             complement(1397..2284)
                     /locus_tag="U4B05_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN2049443.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2373..3596)
                     /locus_tag="U4B05_000278"
     CDS             complement(2373..3596)
                     /locus_tag="U4B05_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2049444.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3613..4659)
                     /locus_tag="U4B05_000279"
     CDS             complement(3613..4659)
                     /locus_tag="U4B05_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2049445.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4823..6550)
                     /gene="ptsP"
                     /locus_tag="U4B05_000280"
     CDS             complement(4823..6550)
                     /gene="ptsP"
                     /locus_tag="U4B05_000280"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN2049446.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6550..6816)
                     /locus_tag="U4B05_000281"
     CDS             complement(6550..6816)
                     /locus_tag="U4B05_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN2049447.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7295..9529
                     /locus_tag="U4B05_000282"
     CDS             7295..9529
                     /locus_tag="U4B05_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2049448.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9677..9997
                     /locus_tag="U4B05_000283"
     CDS             9677..9997
                     /locus_tag="U4B05_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2049449.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10202..10426)
                     /locus_tag="U4B05_000284"
     CDS             complement(10202..10426)
                     /locus_tag="U4B05_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN2049450.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10624..12204)
                     /locus_tag="U4B05_000285"
     CDS             complement(10624..12204)
                     /locus_tag="U4B05_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN2049451.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12605..13981)
                     /locus_tag="U4B05_000286"
     CDS             complement(12605..13981)
                     /locus_tag="U4B05_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2049452.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14109..15227)
                     /locus_tag="U4B05_000287"
     CDS             complement(14109..15227)
                     /locus_tag="U4B05_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2049453.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15369..15803
                     /locus_tag="U4B05_000288"
     CDS             15369..15803
                     /locus_tag="U4B05_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049454.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15881..16414)
                     /locus_tag="U4B05_000289"
     CDS             complement(15881..16414)
                     /locus_tag="U4B05_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN2049455.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16508..17686)
                     /gene="mutY"
                     /locus_tag="U4B05_000290"
     CDS             complement(16508..17686)
                     /gene="mutY"
                     /locus_tag="U4B05_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN2049456.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17679..18476)
                     /gene="recX"
                     /locus_tag="U4B05_000291"
     CDS             complement(17679..18476)
                     /gene="recX"
                     /locus_tag="U4B05_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN2049457.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18583..19965
                     /gene="rlmD"
                     /locus_tag="U4B05_000292"
     CDS             18583..19965
                     /gene="rlmD"
                     /locus_tag="U4B05_000292"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2049458.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20139..20717)
                     /locus_tag="U4B05_000293"
     CDS             complement(20139..20717)
                     /locus_tag="U4B05_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN2049459.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21039..21509
                     /locus_tag="U4B05_000294"
     CDS             21039..21509
                     /locus_tag="U4B05_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN2049460.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21617..22843)
                     /locus_tag="U4B05_000295"
     CDS             complement(21617..22843)
                     /locus_tag="U4B05_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2049461.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23597..23797)
                     /locus_tag="U4B05_000296"
     CDS             complement(23597..23797)
                     /locus_tag="U4B05_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2049462.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23931..24539)
                     /locus_tag="U4B05_000297"
     CDS             complement(23931..24539)
                     /locus_tag="U4B05_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN2049463.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24624..25094)
                     /locus_tag="U4B05_000298"
     CDS             complement(24624..25094)
                     /locus_tag="U4B05_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN2049464.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25142..26056)
                     /locus_tag="U4B05_000299"
     CDS             complement(25142..26056)
                     /locus_tag="U4B05_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2049465.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26202..27005
                     /locus_tag="U4B05_000300"
     CDS             26202..27005
                     /locus_tag="U4B05_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN2049466.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27183..28130
                     /locus_tag="U4B05_000301"
     CDS             27183..28130
                     /locus_tag="U4B05_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN2049467.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28198..28491)
                     /locus_tag="U4B05_000302"
     CDS             complement(28198..28491)
                     /locus_tag="U4B05_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN2049468.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(28519..29184)
                     /locus_tag="U4B05_000303"
     CDS             complement(28519..29184)
                     /locus_tag="U4B05_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN2049469.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29324..29956)
                     /locus_tag="U4B05_000304"
     CDS             complement(29324..29956)
                     /locus_tag="U4B05_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2049470.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(29963..31030)
                     /locus_tag="U4B05_000305"
     CDS             complement(29963..31030)
                     /locus_tag="U4B05_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2049471.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31027..31758)
                     /locus_tag="U4B05_000306"
     CDS             complement(31027..31758)
                     /locus_tag="U4B05_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN2049472.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31927..32406)
                     /gene="greA"
                     /locus_tag="U4B05_000307"
     CDS             complement(31927..32406)
                     /gene="greA"
                     /locus_tag="U4B05_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN2049473.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32542..33717)
                     /gene="mltG"
                     /locus_tag="U4B05_000308"
     CDS             complement(32542..33717)
                     /gene="mltG"
                     /locus_tag="U4B05_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN2049474.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            33921..35090
                     /locus_tag="U4B05_000309"
     CDS             33921..35090
                     /locus_tag="U4B05_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2049475.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            35415..35663
                     /locus_tag="U4B05_000310"
     CDS             35415..35663
                     /locus_tag="U4B05_000310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049476.1"
                     /translation="MIIEINNEILEKNVYEYLIVTLFIPLSLFTVTKGPPCFSTVAFF
                     ELTVTVNPLYRNTGKTIVLPFEDLNVNVISSLFGEKLL"
BASE COUNT        10768 a         7179 c         5900 g        11866 t
ORIGIN      
        1 tgtccattat ttcaaaattt gttatagtct ttttattcaa agcgtaaaag gagatcctat
       61 gttaaaagaa aaaatgaaaa ctctccccat gctgccgggt gtctatctta tgaaagaccg
      121 agatggtaag gtcatctatg ttggtaaagc aaaacggctc aaaaatagag taagcagcta
      181 ttttcatcag aataaacagc attcaaaaaa agtattacgt atgattcatc atataacaga
      241 ttttgatttc gtcgtagtag acacggaatt agatgcttta ttgctcgagt gtcaattgat
      301 ccaacattac cgtccttttt ataaccgaca aatgaattat ttcagtaatt ataattatgt
      361 acatattacc aataagggat ttgtattaac agatactcct acggctcgaa catacgggcc
      421 ttttcgtctc tataaaaaaa tgccgtctat tttgcgtata atggaagaaa cttatcaaat
      481 gccttggttg tctgaaatta gtttgttagc tttgcgagtc cagcttcctg acctacagga
      541 aatgactttt gaacagaaaa aaaaggaact ccaagggctg tttcaaggcc gaaataaaaa
      601 gctactgact tacttgaaaa aaagacaaca acatttcatc tatcagttga atttcgaaaa
      661 agccggtatg cttcaaaaag atatcgaatt agttacttat tttattagac ggatccagga
      721 acaaaagcag tttttaagaa caccatcgct caccttcagc atgccattag cagcagatga
      781 gagtcagaaa aagcactacc tcgtttgtta tggtcaactt gcagaaacaa tgattacctc
      841 tggtgacgtt tctcctgact tctattatga aaagaaagaa gctcatttat ctttgaaacg
      901 tcaattgtca aaagaagaaa tcgatcctgt acaaatcttg atcagttacc gcaaaaaatt
      961 agaaaaagaa cagtttgaaa ttgaactgtt aaacaaaaaa gaagctgaaa agcaactaaa
     1021 ttagttgctt ctcggcttct tttaggcatc ttcaacttca aatgtcaatt atcctaaaat
     1081 ttattggatt aagtcataga tttccatcgc tacgatatcg atattatcaa attcgtattg
     1141 ctgatgagta tctgtttcag ttaattcaaa tgtatcggtc gctttatcat aagttactgt
     1201 acacttctct acgccttctt tttcaaaacg gcgaacttgc agttcattat ctgctgactc
     1261 gaccatagcc tccagtcgtt ttatgattgc gactaattgt gaatgtgtca tcggaggtcc
     1321 tccttttctt atttctgtta tattgtatca aaagcatagc ctacttgcat tgattttctc
     1381 taaaaaaact cgttttttat gatttttttt ctggtatttt tccttcaggc caccaaagtt
     1441 tgatcaaagc ttgggttccg tcattctcaa aacctttatc tgccaactct tcatatagtt
     1501 tcaatgcttg ggtagttgct ggcaatacaa gatccatttt ttctgcttct tctaatgcaa
     1561 tctttaaatc tttgataaaa tgtttgacaa aaaaaccagg tgtgtaatct tcttttaaaa
     1621 tacgaggaga ataattgctt aaagaccagt tagctgcact tccacctgaa agcgtctcaa
     1681 tcactttctt taaatcaagt ccgttttttt gtgcatagac aagcatttcc gtcattcctg
     1741 tcatcgtacc tgcaatcatg atctgattag ccatttttgt atgctgacct ttcccagcag
     1801 aaccatgcaa tgtaaatgtc gtaccaaatt ctttgaataa aggcagtact tgatcataaa
     1861 cggcttcttc tccaccgacc atgatcgtca gtgtaccatt ctttgctcct aagtcaccac
     1921 cggataccgg tgcatctaac gcatctgcgc ctttttcttt tgctgttttt gcaatttttt
     1981 ctgccaggct tggtgtactt gtagtcaaat caactaaaat atgtcctgag atatctgcag
     2041 aaaaaatccc cgactgtcca tagtagattt cttctacatc tttggggtaa cctaccattg
     2101 taaagagaat attactttgt tctgctaccg cttttggtgt atctgcccag atagctcctt
     2161 ctgttactaa aggatcagct tttgcttttg tccgattata gacagctact tcgtgtcctg
     2221 cttctagtag atggcgtgcg acagcacttc ccatgacgcc tgttccgata aaaccaagtt
     2281 tcattttttc gacctcctaa aaatcaaaaa tccttgcttc tatgctagta tacgctagaa
     2341 acaaggattt tttcataaaa aatgtttgaa ttttatcttt taaatgaatt taattttatt
     2401 ttgattcgtt caatcgagtc ttcttcttct tttcttcgca tctcttcttc atgataaaca
     2461 accatgtctt gataaaattc gatcatagct tcgccgaaat gtgtagatga aatatcataa
     2521 agtttagctt ctaaaacttt tggatcttta ggaacttcac tggaaatata atcaataagt
     2581 gctggtgcaa agtcttcgtc ggtttcaaaa gttttcccta atgaaggatg atcgaacaat
     2641 cggttcaaat aagcatttcc ttccacgaca caaggtgttc cggctgccat cgcttctgta
     2701 taagtaagcc cttgtgtttc tgaagtagaa gcactgacaa aataatctgc cgctttataa
     2761 taaagtgcta catcattgtt gtccacttct cctgtgaact gcacaaattc ttctaactgc
     2821 agttctgaga tcaattcctc taactcttct acataaggtc cttttcctac gataactaat
     2881 ctggcgttag gataagcgga gatgacctga ggaaacccat taatcaaggc ttggatattt
     2941 ttctcataag aaatcctgct taaagaaaga atcatcaaat ggttttcctg aagacctagt
     3001 gactccctta atttgctgat atcctgctga gtgatatcag aacgtttgaa tttttcaatg
     3061 tcgataccag taggaatcac gcgaagcggt gctattacac catagctgcg taatgtatca
     3121 ataacacgtt cacttggaca aactacacct gtcgtatgat ttgcaaataa tctagacaag
     3181 tattttacat gggattgcct gatcactttt cctttaccga tataatgaag gtagtcttca
     3241 tacatcgtat ggtaagtatg gatcagaggt attttcaact tcttagctac catcttccca
     3301 agtatccctg ctccgaattc tgtatgggta tgaataagat ctagttctag ttctttggca
     3361 accaaataag cataccacat gcctctaaca acaactcttc gatccttgaa agaaataaat
     3421 ggtacacttg gcattcgaat cacatctttt tccaacttat ccgcattagg atcggtcgtt
     3481 gtaaaaatat agacttcatg cccatgtctt tctaattctt gttttaatgt tttgatcgat
     3541 gtagcaactc cgctgacttg cggaaagtag gtatcggtaa aaaagccgat tttcatgttt
     3601 ttcgcctcca aactagtcct tgacctgatt tacttttttt aatttgctag agactttttc
     3661 attcagtaca ctttcgtata catttttaag ttcttcaccg attttattga tacttcgctc
     3721 ttttgctgtt tgataaccgg cttgtgtcgt actcggtagt tcttgattgg taatctgttg
     3781 gatcagtcgg ataaattctt cgtttgtctt ccccatatag cagttcttgc tatcttctaa
     3841 ccagccatta tacactggaa tatctcgaac aagtacgttt tgtttgcttg ctaaagcttc
     3901 taaaacaacg atcccttctg tctcttcata agaagggaaa aagaatagat ctgcacctga
     3961 atacgcacct tctatgatct ctccacgaat gtaaccagga aacaggacat tttcaggatg
     4021 atcttctttt acaattttcc ggatgttccg aggaatcgag tacatgggaa catgaccaaa
     4081 ccaaataaat gtgtattcgg gcatcttctt agcaatttct acgaaatcaa tgattccttt
     4141 acgttcgaaa aacaaaccga cacaaatgat tactttttga tccggagaca gtttgaaata
     4201 ttctctgaac ttttgttctt tcattggatc agggtgatat ttctctaagt cgatcccatt
     4261 ggaaatagct tgaatcggca cacggatacc atagctttcc aacaactgtt ttgaataagg
     4321 tgttggcgtg atcagatagt ccgctttttg gtacaatcct accagatatt tttttacgat
     4381 aggagaaagc tggttcgatc tgataaagga atttcggaaa tcttcctctg tagaatgcgc
     4441 atgatagaca actttttttc cttctcttct ggctttattg accatattat gactattgat
     4501 tccgtatgta ttgatgtgca ggatgtcata atcttctttt tcatctagtg tataagagat
     4561 accgacttcc gtcagagcac gtttttgatg atcaagcgca cgcccgattc cagattttgc
     4621 aagcatcttt tcactttcaa aatataataa taccttcacc ttacgtcccc cttcacttat
     4681 atagtataac atagcctaca aactgtcact caaacttaag ccctattttt gtctattctt
     4741 actgacaaaa acgttttctt aaaaaagaac aaaaaaactg gcgtagctct tttacaagcc
     4801 acaccagctt ttgatacgtt gtttatttta catattcatg aacaagttca acgacttctt
     4861 ccatcatatc gcattctttc aatgcacgat cagcaagttc agccattttc gctgtgtcca
     4921 agcgtttcat taaactacgc gttttaagaa tagatgtcgc actcattgag aactcatcta
     4981 gacccatacc tactagcaat ggaacagctg tttgatctcc agccatttct ccacacatac
     5041 ctgcccattt tccttcagcg tgtgctgcat caattacatt tttgatcaaa cgtaaaattg
     5101 atgggttata tggttggtac aagtaagaaa cgcgttcgtt catacggtct gctgccattg
     5161 tgtattgaat caaatcgttc gtacctacag agaagaaatc tacttctttt gcaaacttgt
     5221 ctgctaagac tgctgctgct ggaatctcga tcatgatgcc gacttggatc gtatcagata
     5281 cttccttgcc ttcacttatc aatttttgtt tttcttcttc aaagatcgct tttgctgcgc
     5341 ggaattcttt gagtgtagct accataggga acatgatacg taaattacca tgaacagatg
     5401 cacgaagcaa tgcacgcatt tgtgtacgga acatttcgtc gccttgttca gataaactga
     5461 tacgcaatgc acgatatcct aagaatggat tcatttcatg aggtaattgc agatatggta
     5521 gttctttatc tccaccgatg tccattgtac gaacaactac aggttttcct tccatacctt
     5581 ctaatacagc tttgtaagct tcatattggt catcttctgt tgggaagtct ggagaatcca
     5641 tatacaggaa ttcagtacgg tacagaccaa ctgcttctcc accattatta tggacaccaa
     5701 ctagatcttt tggtgttccg atgtttgcag ctaattcaaa atgcttgccg tcagctgtga
     5761 cagtttctgc attttttaat ttttcccatt ctgctttttg tgctgcgtaa tctgcacctg
     5821 ctttttcaaa ttcagctttt tgttcatctg taggatcgat gattacatcg ccttcgatcc
     5881 cgttgacagc caaaatatcg ccttctttga ctttagcagt aatttctttt gtcccaacga
     5941 ttgctggaat ttctaaagaa cgtgccatga tagcagaatg tgaagtacgt ccaccaatgt
     6001 ctgttacaaa cgcttttaca aaattgcgat ctaattgtgc tgtatcactt ggagtcaagt
     6061 catgtgcaac aacgaccact tcttcgttga tcattgatgg atttggtaaa gtaacgccta
     6121 ataagtgcgc taaaatacgt ttagcaacgt cacgaatatc tgcagcacgc tcttgcatat
     6181 aagcattatc ttccattgct tcgaacatac cgatatacat atccgttact tcttttagcg
     6241 ctgattcagc atttacgctg ttgtctttaa tattttgctt gatttgacct accatttctg
     6301 gatctgaaag aaccatcaaa tgggcatcaa atacttgcgc ttctgcttca cctaagcttt
     6361 gcgctgcttt ttcacgaatt tgctgcaatt cttcagtaga tttagctaat gcagcatcca
     6421 aacgagcttc ttcagccgca gtatcttcta ctgttgcctt actgaatgtt aaatccggtt
     6481 gaactagcag gtaagctttt gcaacggcta caccgtcact agcggcaatc ccttttagca
     6541 tttcaaccat tattcagata agccttcttt cttcattgtt tcaacgattg ctgccattgc
     6601 atcagcttcg tcagcacctt ctgcagtaat agtaacatca gaaccttgac ctacacctaa
     6661 agacataacg cccatgattg atttcaagtt tactgattta cctttatact ctaagtttac
     6721 atcagagtta aatttgcttg ctgtttgtac taatagtgta gctggacgag cgtggatccc
     6781 agtttctgct actacgtgaa attctttctt ttccatatta atcggtctcc tttaagtgag
     6841 tgttgttttt atgtgagggt tttctttttt agtaattcag agaatcctaa caaatgccac
     6901 cctttcatct gtaaagatta acattttttt tcgttagata caacagtttt cattcaattt
     6961 cagataaaag tattcaaatt attccggatt ctttttcatc cttttcttca cattttataa
     7021 caaacgtgtt tatttccttt ttcttgtcgc tttctcttct cctgacgttt atcattcttt
     7081 cctttgaccc tctttgtttt catatgaaac gaattacttg cttccctttt tatttttagt
     7141 ataataagag tcaaaggtca ggaatggtca atagagattt caccttttct acctcataaa
     7201 tctcttaaaa aactaggggt tattctttta cctcatgaaa aacgagatgt aaaatatgga
     7261 tgtacccatt tttgtgaaag gacgtgaatg attcatgctt tgtcaaaact gcggcaagaa
     7321 tgaggcaacg attcacttgt atgccactgt caatggtcag cggacacaac tcgattactg
     7381 tcagagctgt tatcaaaaaa tcaaaaatca acaaaatgga ggtctgacta atatggccca
     7441 aaatgatcca tttggatttg gcagtttgga cgatcttttc cgttccatgt ctcgccaaat
     7501 gcaacagcaa ggaaattatg agcaaacacc accaacacaa tttggcggaa acaacaactt
     7561 caatggtggt cagccacctc aaggcaatac agaaggttta ttgggagaat acggaatcaa
     7621 tatcacagaa caagcacgac aaggtgatat cgatcctgtg atcggacgcg atgacgaaat
     7681 caaacgagtc attgaaattt taaatcgccg aaccaagaac aatcctgtct tgattggaga
     7741 accaggtgtt ggtaaaacag cagttgttga aggtttagca caaaaaatcg tagatggtga
     7801 tgtaccgcaa aaattaatgg acaaagaagt cattcgttta gatgtcgtat ctttagtcca
     7861 aggtactggt atacgaggac aatttgaaga acgtatgcaa aaattgattg aagagatccg
     7921 tcaagcagaa aatgtgatcc tattcataga tgaagttcac gaaatcgtcg gtgctggttc
     7981 tgctggtgat ggaaatatgg atgctggtaa tattttaaaa ccagctttag ctcgaggaga
     8041 attgcaaatg gttggtgcta ctaccttaaa tgagtaccgc atcatcgaaa aagacgctgc
     8101 tttagaacgt cgtatgcaac ctgtacgggt agatgaacca actgtcgaag aaacgatcag
     8161 tattttgaaa ggtttgcaaa aacgttatga agactatcat catgtaaaat atactgatga
     8221 ggccatcgaa gcagccgcaa ctttatccaa tcgttacgtc caagatcgct tcttgccgga
     8281 taaagccatc gatttgctag atgaaacagg ttctaagaaa aacttgacga tccaaatcgt
     8341 tgatcctaaa acaatcgaga aaaaactgca agaagcagaa gaacaaaaag ttttagcttc
     8401 tcgagaagaa gatttcgaaa aagcagctta ttatcgcgat cagatcaata aacttcaaaa
     8461 aatgaaagaa cgtcagctca ctgaagagga aacacctgtg attaccgaaa aagatatgga
     8521 aaaaatcgtt gaacaacgga caggtattcc agtgggtgaa ttaaaagaaa aagaacagac
     8581 acaactgaaa aacttagcag atgacttgaa agctcatgtc atcggacaag ataacgctgt
     8641 agatcgagta gcgaaagcga ttcgccgaaa ccgtgttgga ttaaacaaac agaatcgtcc
     8701 aatcggctcc ttcttatttg ttggaccaac tggtgtaggt aaaacagagc ttgcgaaaca
     8761 acttgcctac gaactattcg gttcacaaga ttcaatgatt cgtttcgata tgtctgaata
     8821 tatggaaaaa catagtgtct ctaaattgat tggttctcct ccaggttatg tcggatacga
     8881 agaagctggt caattaacag aaaaagtacg gagaaatcct tacagtcttg tcttgttaga
     8941 tgaagtagaa aaagctcatc cagatgtttt gcatatgttc ttgcaaatct tagatgatgg
     9001 tcgattgaca gatgcacaag gaagaacggt cagctttaaa gatacgatca tcattatgac
     9061 aagtaatgcc ggaactggta aagtagaagc aaacgttggt tttggtgcgg cacgcgaagg
     9121 tgttactcgt tctgtattga accaattaaa caactacttt acaccagaat ttctgaaccg
     9181 ttttgacggg atcatcgaat tcagtgcatt atcaaaagaa aacttgatga caatcgtcac
     9241 actaatgcta gacgatgtca accaaatgct ggcagcacag caacttcaca tcgaagttcc
     9301 tacgaatgtc aaagaaaaat tagttgactt aggctatgat ccatctatgg gtgctcgtcc
     9361 tcttcgacgc acgatccaag aacaaatcga agatggtatc gctgaatttt atcttgatca
     9421 cccttctatc catgagttga aagcaaaact ggataaagat ggaaaaatca tcgttacttc
     9481 taaaccagaa cgtttggcta aagaatcagc cgaagaaacg gctgaataaa tccagtttta
     9541 tagcgtgaga cggtatttct ttgagaaatg ccgtctcatt cttttttcag aaaagtcaaa
     9601 atactgttca ttttataaaa aagaaattat aataagagaa acaagcaatt aaaaatacat
     9661 aaaagaggtg agcgccatga aattggtaaa tgtaacaaac agccattccc gtcttgtatt
     9721 aaaccagctt gaaaacactg atgcccatct agtcaaggtc tacacagccg gaaataccac
     9781 tattgtctac actgaagccc ctgaacataa tgaaattctg ctgatcaatg acaaacgtaa
     9841 aatccagccg aaagaaatcg aagatgctaa aaattatttc ttagataaaa tcaaagacgc
     9901 cgtttaccac gaggatgaaa tcaaagtaat cgaattggca ggccttgttg aaatctcgat
     9961 tccaaaacat gaattaggcg ctacattagc cgtgtaaaca aattttgaac ataataatta
    10021 aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    10081 cgaaagcaga agcaactcct tcggaaataa gctgaaattc acgaaaattg cttttcaaat
    10141 ttttgtgaat tccagcttat ttcttggagt taaacgcttc tgtctcagcc tctttttctt
    10201 tctagctttt tgatttttct cttttctttt gtacttttct cttttcattt tcagttctac
    10261 gcgtgtcttt atttttaact ttttcctctt tatggatgat tgatcgtact ttttgtgcaa
    10321 tttgctcgtt tttgtcccag tcttttacat ctaataatcg acataatcgt aacatctctt
    10381 cttttttctg tttttttctt tcacgtcttt gccattctgt ttccatgtgt cacactctcc
    10441 tttctttaat catatgtacg caaaataatt tttatatttt tttgattaga catttctatg
    10501 attttaatat agtaaaaaga gccgtcacgg aaaatccgtc acggctctaa acaaaatatg
    10561 agaggctgtt tttgtttggc tattcttatt acttgatgca gggcagcttg tgtcaagact
    10621 tctttacatc aaacttttta attttacatt gggatattta tccgcaaacc agcgttctgc
    10681 aaattggttc tcgaataaga acaacggttg gtcgaaacgg tcacgagcca agatattgcg
    10741 acttgaactc atcttctcat ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc
    10801 cattggagac atgattactt cggcgttata ctcatttaac atacgatatt ggaatacttc
    10861 gaattgtagc tgaccaacag ctccaatgat atattcttcc gttagatatg ttttataaag
    10921 ctgaatcgct ccttcttgaa ctaattgata gattcctttg tggaaggatt tttgtttcat
    10981 gacatttttt gcagtcactt tcataaacag ctctggagta aatgaaggga gttcttcata
    11041 agcaactttt agttttcctt cgaacaatgt atccccaatc tgataattgc ctgtatcata
    11101 tacaccgatg atatcgcctg ctactgcttt ttcgacgttt tctctcgaat ctgccataaa
    11161 ttgtgtgaca ttgcttaatt tcagtttttt atttgtacgt tccaaccaga catccattcc
    11221 tcgttcgaat gttccagaac agattcggac aaaagcgatt cggtcacgat gggctggatt
    11281 catatttgct tggatcttga atacgaaacc tgaaaattct tcctcataag gacttaccat
    11341 ctgatcatct tgtgttttgt gtccataagg agctggtgcc aattcaacga aagtctctaa
    11401 aaaagtctgt acaccgaagt tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc
    11461 acgagcaatt ttttcttgat taaatgcatc tccagcctct ttgattagtt ctacttcttc
    11521 taagacttgt tcatataagg aattttcagt tagaggatga ttttcgggaa gcagtccatc
    11581 ttcatccaat tctactaatc gttcatcctg ataattttcc ggacgataaa actctacacg
    11641 attatggtag atatcataaa gaccttctag ccctttaccc ataccgattg gccaattcat
    11701 cggataagat tcgatatcta atagctcttc taattcttct aacaattcca atggttcacg
    11761 accatcacga tctagtttgt tgataaaagt aaatatcggg atgccacgtt tttttacaac
    11821 ttggaataat ttctttgtct gtgcttcgat cccttttgca ctatcgatta ccatgaccgc
    11881 actatccacg gccatcaaag tacgataagt atcttcagaa aaatcttcat gtcctggtgt
    11941 atccaagata tttactcgtt tcccttgata atcaaactgc ataacagaac tagtaacaga
    12001 aatcccacgt tgtttctcga tttccatcca gtctgatttg gcaaaattac ctgttttttt
    12061 gccttttacg gtcccggctt gacggatagc cccaccaaac aatagtaatt gttctgtaat
    12121 cgttgttttc ccagcatccg gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg
    12181 ttgctttaat tttggattat tcataaccta actccatttc ttccattcaa ttatttatat
    12241 tttctctagg cacagattgt ccgatctctt aaagaaagac aatttcatat agggctgtga
    12301 caaaagccgt gtagcagtct ctttttccga gtaaacggcg ttctagagac agcctatcag
    12361 caataagccc acccaagcca aaaatacgaa aagctgtttt tgcttggctg ctcttattac
    12421 ttaatgcagg atagcttttt cacaaccttt gtcaactact acaactgaat cagtatagca
    12481 tggattcagc gaatgaaaaa gccaccaaac cataagagtc aaatattttt ttgtcagctg
    12541 actaaaagaa aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt
    12601 ccaattatct tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt
    12661 caggttccgc ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag
    12721 aaccttccat ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc
    12781 cttcatccgg aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac
    12841 tcatatgcag atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga
    12901 tgatatattc gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat
    12961 cgatttctcc gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc
    13021 catattcatc taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat
    13081 catcaataaa gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta
    13141 aattatcaaa gccgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga
    13201 ctactttatc tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt
    13261 cattcacatt ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga
    13321 ccatgacttc tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt
    13381 cttcattatt caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt
    13441 ttgaatcttc atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag
    13501 aaagcagcca gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga
    13561 actttgcgac ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa
    13621 caatcgatac ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg
    13681 tgccgccacc taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag
    13741 acaaaatgtt gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct
    13801 gcaatatttt taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc
    13861 gattcttatt gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg
    13921 tcaaaacgat caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca
    13981 taatcgcatt ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac
    14041 tatagccacg ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact
    14101 aaaactacct acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg
    14161 acgattttga ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag
    14221 aagataccaa gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat
    14281 ggatggattt tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt
    14341 tggacgatta gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt
    14401 actagaaatg caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat
    14461 cccgcaatga ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat
    14521 gtgaaggtac caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt
    14581 ttattcagtt gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt
    14641 tggatgtttg gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct
    14701 gtaatgatta ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat
    14761 actccactaa ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat
    14821 ttattgatat ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct
    14881 acctgtttga tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt
    14941 gggatgacac taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg
    15001 acagcccata ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa
    15061 tagtaaagaa agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc
    15121 ggttcgaaaa taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat
    15181 tcaagcgacc aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct
    15241 ttctttctta ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt
    15301 gaaaacttgc catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg
    15361 gctagaccat gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg
    15421 tacggatcag gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag
    15481 acaaagatga agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag
    15541 gaactgtacg tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc
    15601 tttctgaata cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg
    15661 ctaagaatca aggctataat acgattttat taggggcaca gtccacagct gaaacttttt
    15721 acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc atgcctcata
    15781 tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt
    15841 tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct
    15901 tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga
    15961 tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat
    16021 tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag
    16081 gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa
    16141 aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat
    16201 tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc
    16261 catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt
    16321 ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga
    16381 tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt
    16441 gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga
    16501 gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca
    16561 tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt
    16621 tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat
    16681 gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa
    16741 agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca
    16801 gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca
    16861 cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa
    16921 aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct
    16981 ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa
    17041 aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta
    17101 gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg
    17161 cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc
    17221 tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac
    17281 ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg
    17341 tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc
    17401 gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat
    17461 ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg
    17521 tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat
    17581 tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa
    17641 attcttttgt ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt
    17701 tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt
    17761 acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct
    17821 tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc
    17881 gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc
    17941 aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt
    18001 gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt
    18061 ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc
    18121 atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt
    18181 aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt
    18241 cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg
    18301 aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct
    18361 tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat
    18421 aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac
    18481 atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat
    18541 atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc
    18601 aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc
    18661 ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta
    18721 gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa
    18781 acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa
    18841 ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca
    18901 ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg
    18961 gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact
    19021 caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc tataacagac
    19081 tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat
    19141 aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg
    19201 gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa
    19261 ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca
    19321 atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac
    19381 ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct
    19441 cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa
    19501 gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc
    19561 ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca
    19621 atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc
    19681 gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc
    19741 atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac
    19801 cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt
    19861 aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact
    19921 gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg
    19981 aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt
    20041 tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag
    20101 ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc
    20161 acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat
    20221 ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct
    20281 ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc
    20341 caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc
    20401 caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt
    20461 tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta
    20521 tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag
    20581 tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc
    20641 gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac
    20701 gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta
    20761 ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa
    20821 agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat
    20881 caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat
    20941 aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc
    21001 ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc
    21061 gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg
    21121 gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga
    21181 tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac
    21241 ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca
    21301 tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa
    21361 tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc
    21421 atcgcctggc aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat
    21481 tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca
    21541 catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta
    21601 accaaactat ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac
    21661 cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg
    21721 tatagtactg acttgtatct tctgtttgct gataataggt attcggatct tggtactgat
    21781 catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac
    21841 cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt
    21901 tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga
    21961 tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt
    22021 cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat
    22081 caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt
    22141 ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt
    22201 cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat
    22261 aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca
    22321 aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca
    22381 tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg
    22441 aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc
    22501 cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt
    22561 gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta
    22621 atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta
    22681 ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt
    22741 ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga
    22801 atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca
    22861 cattttctaa aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata
    22921 agaaaacttt aaaaaacatt tttgtgtaac gattattatt ttttaaaatt tctcaaaatt
    22981 cttaactatc ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat
    23041 cgttttatct ttttttatgt attttttcca gaaaaagcga acaacaaggt attttccaaa
    23101 taatcgtgca gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac
    23161 cgttcagatt atcattcgat ttcacgacaa tttcttccta tgactggtcc ttttgttaaa
    23221 cccatcttct attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta
    23281 gtgattgcat taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat
    23341 tttcttttaa agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa
    23401 tggtctttta ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa
    23461 aatgttcctt gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc
    23521 caaaaaatct aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc
    23581 tttattcaac aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt
    23641 caacaatcgt gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa
    23701 ttgctgagaa atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt
    23761 tttcattgtt gaaccattta accgttccgt gttccataaa tgtatatcct ccaaaaaagt
    23821 tcttgcaagt aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg
    23881 ctcttttatt ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct
    23941 gcttcgaaac gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa
    24001 tctggtcgga catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga
    24061 attggtgtct ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat
    24121 tttccatttt ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt
    24181 ttgaattctt ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt
    24241 tctcctgtag gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta
    24301 gcatgtccgc caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata
    24361 tcagataata attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgaa
    24421 tttaaattcg tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg
    24481 tcaatataag gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata
    24541 aaaaaattcc tcctcaatgt tttcttaata atgtctacat tacaaagagt aacaaagaga
    24601 aggaaattct tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca
    24661 aaaacctcag tttctttaaa aataaacaac ttgctgaaaa tatagttcgc aaggataaca
    24721 ataatttgag tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat
    24781 accattgccc ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat
    24841 tcaataagtg catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt
    24901 acaaaagcaa acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa
    24961 tcaaaatatt ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca
    25021 aaaaagagat agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt
    25081 cgatactgct tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat
    25141 ttcaataact ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct
    25201 ctgcttggat cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag
    25261 gatatatctt ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa
    25321 taattatctt ttcgctatac tgccaaatat ggacatgatg aacatccttt accccatcga
    25381 ttgcttcaat ttcttgttcc attttttcat agtcgatagc aggtgcactt tgtatcaaaa
    25441 tagcggcagc ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca
    25501 gtgtaataac tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa
    25561 tcacacttac cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca
    25621 aactttcatc tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa
    25681 taactgctac tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga
    25741 atgcttcaac ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac
    25801 tcaatatttc taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca
    25861 ttttttgtgc cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat
    25921 ctgaaacaag agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca
    25981 ccgcattcaa aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca
    26041 ctattccttc ctccatttta tttatattgg atagtcttta gacaagaata acattttttt
    26101 gatatttcac caatcattca gctgtttcct taaagcactt gtatttcact tgatacttcg
    26161 ctagaattgc aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa
    26221 ttatcagttc ctttatacga ttcaaagacc taaaggaggc gagaaatgtc ccattttgga
    26281 aagccattgt ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag
    26341 tttttcaagt cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg
    26401 attttacaat ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa
    26461 caaactccat tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta
    26521 ctgatttgat ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc
    26581 ttcctaatac aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca
    26641 ccaatgaatc tttaaaaaac ttaacaggta agcagctccg ttctttttta agtgaagcat
    26701 ctataccact ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga
    26761 atctgctgat cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac
    26821 taacaaaagc tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta
    26881 ctataattgc aacaatcttg atggtctttc ttccttcttt tgacactagc gccttcattg
    26941 cttttgcagg aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca
    27001 attaagaaat aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac
    27061 atttttttgt cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa
    27121 ggcgcttcaa agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa
    27181 ctatgtcaga aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa
    27241 tccatactat agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa
    27301 atgtagagat cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta
    27361 ccttgaaatg ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt
    27421 caaagaaaca tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc
    27481 atgtcttagg aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaggcacaga
    27541 gtgacatgaa atggttaggt tcatcggtcc attaccaatc tatccaaggg aaaaaaatac
    27601 taatcattgg aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca
    27661 tgaactgcta tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt
    27721 ctttaaatca actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc
    27781 taacggagga aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat
    27841 ctgtattcat caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt
    27901 taaataacca gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac
    27961 aagatcatcc tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga
    28021 cacctgattt tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg
    28081 ccacccatga gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa
    28141 caatattttc aaaagaaaaa agagaaggtg acaaaaattg tgtcaccctc tcttttttta
    28201 ttgtttttta aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc
    28261 atatccttta aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catcaatttc
    28321 cactttcttt acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg
    28381 aacctctgtt ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc
    28441 aatttctgag tcaaaccact tttgtgcttt tggcgttacc gttaatttca tcccaacaac
    28501 ctactttctc ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt
    28561 ctctccaata accagtagat tatcattttc acgaacatat tcatctgctg caggagaagc
    28621 gataaccgtt tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa
    28681 atttaattca gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa
    28741 agaatattta tctgacaact ctaggtaatc taaaatattc tttgacacta aattatgcgc
    28801 aatccttatt cccatgtctc gctcgggatg gacgacgcga tcagctccga tcttttcaag
    28861 tactctggca tgatactcat tttgggcttt tgctaaaata tttggtacgc ccatttcctt
    28921 taccatcaga gtaactaaga tactggcttg aatatcttct ccaatagcga caattacatg
    28981 atcgaaattt cttatgccta atgatcgaag tgtcatttca tcctgtgcat ttgccacaac
    29041 agcatgagtg gctatattca tatattcatt gacccgatct tcattactat caatagccag
    29101 tacttcttga ccagcttcga tcagtgttcg gcagatactt ccgccaaatc gtccaagacc
    29161 tacaattgca aaattttgtt tcaccgtttt cctcctttag tatcttcttt tctcagtata
    29221 acaaagcctt tacaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa
    29281 tagttatttt ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat
    29341 gtttgaatgc atagatagcg gcttgtgtac ggtcttccac ttctagctta gccaatatat
    29401 tggaaacatg tgtttttact gttttcagtg taataaacaa ttcgtccgct atttcctgat
    29461 tactttttcc ttcagaaatc agcattaaaa tttcattttc ccgattcgtt agttcttcat
    29521 gtaatacagg ctcatttcgc cgactcattt tttccatcat ttttgttgtc acttctggtt
    29581 ctaatactcg ctcgccacgt tgcgttgccc ttatagcatc agcaatttca tgtgcggtag
    29641 aggtcttcaa caaataccct gctgcacctg cttcaatggc aggataaact ttttcgtcat
    29701 caataaaact ggttacgata atgattttag cttctggcca atcttttaag attgcttttg
    29761 ttgattcgat accatccatt tcttccatga ctaagtccat taaaatgacg tccggtcgaa
    29821 gtgtcatcgc cttctcatag ccttgtcgtc cattttcagc ttctccaatt acttcaatgt
    29881 cttcttgaat agataaataa aaagagacgc ctaagcgcac catttcatgg tcatctacta
    29941 ataaaacttt tatcatcctt atctatgcct ccttcatcaa agggaccttg atttctacac
    30001 ttgtaccttg acctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt
    30061 ctttgatatt gttcaatcca taacttcccg ttttcgtttc attcatatta aaccctgttc
    30121 cgtcgtcaat aatacgtaac aaaagattat tgtctatttt gtgcaaatat acctctaatt
    30181 cattggcttt agcatgtctt aatgtatttg ataacaattc ttgaacgatt cggaacaagt
    30241 gatcctcaat ggaactagat agttttacat cttctacatc ccatttcagc gaaatctgga
    30301 ttttattttg caattccttc aataactgtt cgataccttg ctttaaactt ttctcttcta
    30361 aattgactgg acgtagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga
    30421 tctctgctac cattttcaac tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt
    30481 gctccgtcaa tgccgacatc atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat
    30541 gcagctcacg ggctaatcga tgtctttcta attctaaaat ttcttctttg gtttgtccat
    30601 ctacatattg gggacggctc gttacaattt gtaattcact agatacttca atcatttttt
    30661 cttttatctt agtgatttcc ttatcaatat ctttgatgta taaatcgtcc gatgcacttg
    30721 ggatcggaag aagcaacaat ttggattcat aattgccaga agaaagtgca cgcagctttt
    30781 cttcgatttt cccgtatttt gtcttttgaa taagtgataa taatagaaag acaattaatc
    30841 ctacacctaa ggaaatggct aacaaatgga agataagcgg aacatataat aaacgtgcac
    30901 gcagtaattc tccccaccaa tgactttgat tgctggcata aaaataagtg aaaagtgaaa
    30961 atagaataat aaggaaagcc gcaatccccg aataaacagc tagcatcgct ctggatattt
    31021 ttcccatcat attcgtatca cctcgacatc tccaatcaag gtattagtaa taatcttcaa
    31081 tctacgaggg ttttcatcat aatcactgct gtagattttg atttgttcat ttttcaaaga
    31141 aaattgctct tcttcaaaca agacatttcc tactaatgtc gcatgctcta atcggatggc
    31201 tacgcccaaa ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt
    31261 atcatctttt ggcaaaagcg tattgcccaa atctatgata gtatcaccag aaatgacggc
    31321 gatgttgata tcgtcccatt catagacgtc gtttccaata cgttggttac cgaataattg
    31381 ctgtcttttc cgctcattat tatgtgtttt taattgctct gtttggatca tcatgatctg
    31441 tttctttcgc caaaaagcat ttttggttaa atctatccct gaaatctcta cacctttcag
    31501 accaataaat aaaacggcaa atacagccat catccataaa gcaggactgt tgactaagct
    31561 aaaaaaaatg accaaacttc ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg
    31621 tttgcgcata gctagatata tattgaaaat accgaatatc actaaaagca aaagtccagt
    31681 attgtttacg atttgccaaa cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa
    31741 acgccaagaa ctattcatcg ctctttctcc ctcctgacac attctcattt catcatacct
    31801 atttttttaa ctgttggcta taggacttta gatgtaagat cgtttgttga aatctaaaat
    31861 acacaaagag aaaaagatct agagtgtatc atcagtaaga tgaactctct agatctttca
    31921 gtcagactac gcttgttcta ctttgacgat tttaactaac atatcgccac ctggagtagc
    31981 aatagttact tcatcatttt tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt
    32041 ggaaattttg ccagaaaacg gatctgcttc agcgcttccg acaatcgtgt actcttcttc
    32101 ttctccatct ggtagttcga tgaatgtaac tgttttacca attgatacct catctgcatc
    32161 tacaccgtcg ttatcgatga tttgagcaaa acgaatcata ttttcaattg ttgtgattct
    32221 tccttcaaca aatgcttgtt catctttcgc tgattcatat tcagagtttt cggaaagatc
    32281 tccgaaaccg cgggcaattt tgattcgttc aatgatttct ccgcgtttga ctgtttttag
    32341 ttcttctaat tctttttcta atttttcttt accttctaga gtcattggat aaactttctc
    32401 taccatgtgt aacgtctcct tttttcgata aaataccgca aaaggtaatc ttttgaggaa
    32461 tatagtccaa aaaagattac cacgcttttc ttcaaatgta aatgtttttc taaaaatgat
    32521 caatgtctag agcctgtttt tctaagaatt atttacatat ttttccacta attccatatg
    32581 ttcttcataa gttttagcga aataaacatt tccagtgctc gtatctgcta cgaaataata
    32641 atagtcattt tctgctggtt ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa
    32701 aggtccaggt ccatagccgg tattggtata caaattatat ggtgaatcta cagctgtatc
    32761 ttcatacgtc actaattctt tgtgttcacc taatgcgtat aaaacagaga tatctgactg
    32821 aagtggcata tttgctttga ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt
    32881 ttcttttacg ccttcttttt caactagaga agctagagtt aaaacttctt gtacagtcaa
    32941 atttttctgc ttgatagctg aataatagtt ttgcatgacc gtattcgtct tgttcaccat
    33001 ttcaatcact agatccttaa gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa
    33061 gtaaccttcc aaacggtatt ttgtgttctc agctttagat gcactttcca acagttctgg
    33121 gaatttttgg tgcaactcgt taaagaaagt ctcatcattc actaaatcta aaaattcttt
    33181 cttgtcttta ccagttacct tcgctacccg ttcagcaatc tgatcgatat cgtacccttc
    33241 tggaatggca attttcccat ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa
    33301 ctgtttgcca atctcgtcaa gagtcatatt aggcgctaac tgataatagc cagcttgaaa
    33361 gccagttaaa tttttaaatt ttgtataata attgaaaaca atcccgcttt taataatatt
    33421 gtccttctcc aaaatctcac caatctgctt gttagaagag ccactcggga tctcgacttg
    33481 gactaattgg tcatctgatt tatctaaagg cttcaacccg ctatctacat aacgatacac
    33541 ggtaaatcca agaaatccgc caataatcaa taatgtaaga gcaatcacta aaatgatctt
    33601 cctcacaatc ttatcttctt ttttacgcag atttctccgc ttgtcgatta tatctgctgt
    33661 agctgcctta ctggcttctt ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac
    33721 aactcctcct tactatcaag ttcgtaactt tattcattat acatgatatt tgaattggcg
    33781 tgaatgattt tatttaaata ttctatcaaa aatgtaagta gtaaatccat tactataaga
    33841 ttaaaataaa tgttatcaag atacaagcta ttttagcttt attctttctg taaaaaagaa
    33901 aggagactaa ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat
    33961 ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga actattcttg
    34021 gatggcaatt ttacaataat gaacactctc agatagttgc ccatgctcaa tcagctcaaa
    34081 aaaagttgat actgactacc aatgaacaac agctcgttga aaaaatcgat caactattaa
    34141 gaaaaaatga ttatattggc tcaatttacg taaagaaaaa taatcgcatc atcatggaaa
    34201 aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa tgatccttct ctttattatc
    34261 agattggttc ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg
    34321 ggcacctttc tttagatact aagctggctc aattctatcc acaaattgct ggcagccagc
    34381 aaattactat ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact gcttctccaa
    34441 ccataccgat gactgatgaa gaagtcgttc aatttgcgat tcagcactta aaatttacag
    34501 attatcagac ctctcattat gattcattga actttaccat attgactgga attttgatca
    34561 aattaacaaa tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa cctttaggat
    34621 tacagcatac aaaattttat gaccaagtca aaaattcatc caatcatgca ctatcctatc
    34681 aaatgtctaa agaggatgat tactttaaac cactgacaga atctgaaaca gagatccata
    34741 acgaattagg gactggtaat atcagtatgt ccgtctatga tttaaatacg ctgtttacac
    34801 aagttttatc tggaaaacta gtttccaaag acttactctt ctcattatgg cgtgaaaatg
    34861 cgaatggaca tccatatagt ggtggggtat atagcggaga taattatatc ctgtcgcaag
    34921 gaaatatcga ctcttttgat acatctgcta tatttagcaa agattcacag gatgctatca
    34981 tcatggaaag caatataaaa gctgacaaaa ctatcaaact gcctactgct gatctaagaa
    35041 accaaatcta tgacctgatg gaaggaacta ctaagttggc aaatagctga tatgatgaaa
    35101 agatagcaaa tactacttct ttgaatagca tttgcttaaa aaaagtactc gactaaacgt
    35161 aggaaagagt gtgggacaaa tctaaaaatc aatttttgct acagacttta attccaaata
    35221 aacagaacag aaagaggtgt tcttctttct gttctgtcca aattattcta agatttctct
    35281 ttaccaacat tatttgacaa agggcctatt aaatctaaaa tagtatcttt agattgctaa
    35341 aatatgtgct agcaaatcat tcatggcttg ttcttatttt taattttatg caataaaaaa
    35401 gaagaacaat tagcgtgatt atagaaataa ataatgagat tttagaaaaa aatgtgtatg
    35461 aatacctgat tgttacttta tttattccac tttcactttt tactgtaaca aaaggacctc
    35521 cttgttttag tactgtagca ttttttgaat taacagtaac cgttaatcct ttataccgga
    35581 atacaggtaa aactattgtt ttgccttttg aagatttaaa cgtaaatgta atttcatctt
    35641 tatttggaga aaagctacta taaataactt gaccatcttc tgtaacttga tggtttttca
    35701 aaagcaattg atc
//
LOCUS       DASJST010000008        34962 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJST010000008 DASJST010000000
VERSION     DASJST010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.008"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="U4B05_000311"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="U4B05_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN2049477.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAVLEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="U4B05_000312"
     CDS             complement(2019..2921)
                     /locus_tag="U4B05_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2049478.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="U4B05_000313"
     CDS             complement(2918..3610)
                     /locus_tag="U4B05_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN2049479.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="U4B05_000314"
     CDS             complement(3653..4294)
                     /locus_tag="U4B05_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2049480.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="U4B05_000315"
     CDS             complement(4332..4646)
                     /locus_tag="U4B05_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049481.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5986)
                     /locus_tag="U4B05_000316"
                     /pseudo
     CDS             complement(4634..5986)
                     /locus_tag="U4B05_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5983..6915)
                     /locus_tag="U4B05_000317"
     CDS             complement(5983..6915)
                     /locus_tag="U4B05_000317"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN2049482.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="U4B05_000318"
     CDS             complement(6912..7688)
                     /locus_tag="U4B05_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN2049483.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="U4B05_000319"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="U4B05_000319"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN2049484.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /gene="carA"
                     /locus_tag="U4B05_000320"
     CDS             complement(10932..12008)
                     /gene="carA"
                     /locus_tag="U4B05_000320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN2049485.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="U4B05_000321"
     CDS             complement(12013..13293)
                     /locus_tag="U4B05_000321"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN2049486.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="U4B05_000322"
     CDS             complement(13312..14238)
                     /locus_tag="U4B05_000322"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN2049487.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="U4B05_000323"
     CDS             complement(14251..15534)
                     /locus_tag="U4B05_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN2049488.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="U4B05_000324"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="U4B05_000324"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN2049489.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="U4B05_000325"
     CDS             complement(16322..17233)
                     /locus_tag="U4B05_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2049490.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /locus_tag="U4B05_000326"
     CDS             complement(17230..17694)
                     /locus_tag="U4B05_000326"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN2049491.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="U4B05_000327"
     CDS             complement(17699..18397)
                     /locus_tag="U4B05_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2049492.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="U4B05_000328"
     CDS             complement(18384..18770)
                     /locus_tag="U4B05_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049493.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="U4B05_000329"
     CDS             complement(18917..20587)
                     /locus_tag="U4B05_000329"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN2049494.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="U4B05_000330"
     CDS             20770..21003
                     /locus_tag="U4B05_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN2049495.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="U4B05_000331"
     CDS             complement(21102..21533)
                     /locus_tag="U4B05_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN2049496.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..22025
                     /locus_tag="U4B05_000332"
     CDS             21612..22025
                     /locus_tag="U4B05_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN2049497.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22063..23211)
                     /locus_tag="U4B05_000333"
     CDS             complement(22063..23211)
                     /locus_tag="U4B05_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2049498.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23333..23929)
                     /locus_tag="U4B05_000334"
     CDS             complement(23333..23929)
                     /locus_tag="U4B05_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2049499.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="U4B05_000335"
     CDS             complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="U4B05_000335"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN2049500.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27159..27590
                     /locus_tag="U4B05_000336"
     CDS             27159..27590
                     /locus_tag="U4B05_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049501.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27635..28984)
                     /locus_tag="U4B05_000337"
     CDS             complement(27635..28984)
                     /locus_tag="U4B05_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2049502.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29054..30217)
                     /locus_tag="U4B05_000338"
     CDS             complement(29054..30217)
                     /locus_tag="U4B05_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2049503.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30346..30669
                     /locus_tag="U4B05_000339"
     CDS             30346..30669
                     /locus_tag="U4B05_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049504.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30852..32201)
                     /locus_tag="U4B05_000340"
     CDS             complement(30852..32201)
                     /locus_tag="U4B05_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2049505.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32270..33223)
                     /locus_tag="U4B05_000341"
     CDS             complement(32270..33223)
                     /locus_tag="U4B05_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN2049506.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33325..34515)
                     /locus_tag="U4B05_000342"
     CDS             complement(33325..34515)
                     /locus_tag="U4B05_000342"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN2049507.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7642 c         6121 g        11248 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctgttttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa aaaaagactc
    22021 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
    22081 gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt gactacgtaa
    22141 gattctttct agttctgtat caacaaactg gaatttctca gggaccacta catcaaattg
    22201 cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg tacctgtttc
    22261 aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat caacaggcat
    22321 tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt tttctatcag
    22381 atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga tgtggactga
    22441 tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc caagtatatc
    22501 atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg ggcgacttcc
    22561 taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga tatataatgc
    22621 taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga ttgctgctga
    22681 aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg tagctctcaa
    22741 tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg caaatttcaa
    22801 aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat tttctggatg
    22861 gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa tgatgatcga
    22921 aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt cctgatctgc
    22981 tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag tatctgatag
    23041 actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga tcattagctt
    23101 agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta ctcctttttt
    23161 tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca agttttttac
    23221 cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt tttcctctcc
    23281 tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt cattattctg
    23341 gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg attcccgcac
    23401 ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta aataaaagag
    23461 cagagtctac accataggca tctgccgttg cacctgtata aaataatccc atgaatccta
    23521 acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag ctgatcgtta
    23581 ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag ccaacaagta
    23641 ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat ggcgttgttg
    23701 gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc accatgccat
    23761 cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt gttaaactaa
    23821 tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg atgattgcta
    23881 acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt ttttcacccc
    23941 tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag accatccgaa
    24001 tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca agacttgttt
    24061 gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt ctgtcaaacg
    24121 taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc tggaatacca
    24181 gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc accgcctact
    24241 ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt tgattctttc
    24301 gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac aagaatatct
    24361 gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat atctttcact
    24421 tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt gaatacatca
    24481 ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg tagctctctt
    24541 acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg tgatttaaca
    24601 attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta cgcttcttta
    24661 cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga aacgattttg
    24721 aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc gattgaccag
    24781 ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa agccattgca
    24841 cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat agcttcccag
    24901 attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat ttgaccaaag
    24961 tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg atttgcatgc
    25021 tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg gtttttgatg
    25081 atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc actcattggt
    25141 gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac atctgtaaca
    25201 actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac ttgcccaccc
    25261 atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc tgcgacttca
    25321 ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc ttgttctgtg
    25381 tatccaatga atttactttc tactttgata tctgtcaata aagcagattg tacccccatt
    25441 gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat ttctttttca
    25501 aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt taattctacc
    25561 gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt cgtcccgttt
    25621 tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt gattgtttca
    25681 ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg ttgaagtact
    25741 tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata taagaatgct
    25801 tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg gcgtaagaca
    25861 taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga aagggcgcga
    25921 atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc atattttact
    25981 tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt ctcaaagttc
    26041 gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt atcaatgttt
    26101 ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga aaatactaag
    26161 ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg atcgtcttct
    26221 gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg accacatggg
    26281 cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag tggcaggcca
    26341 acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt tacataaagt
    26401 ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc ccagtggatg
    26461 gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa catcgtatgg
    26521 tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt ttgggcattt
    26581 gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa tgttgcaaca
    26641 ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc actaggttca
    26701 atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg tgcactactt
    26761 aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca ccatcacagc
    26821 caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat tacctcttaa
    26881 actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg agcaatttga
    26941 ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa aaaacaaatc
    27001 atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa tatctcaatt
    27061 tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag aataaaggaa
    27121 tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata gttggagcgg
    27181 atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta ttagaacaag
    27241 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
    27301 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
    27361 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
    27421 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
    27481 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
    27541 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
    27601 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
    27661 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
    27721 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
    27781 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
    27841 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
    27901 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
    27961 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
    28021 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
    28081 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
    28141 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
    28201 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
    28261 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
    28321 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
    28381 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
    28441 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
    28501 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
    28561 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
    28621 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
    28681 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
    28741 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
    28801 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
    28861 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
    28921 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
    28981 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
    29041 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
    29101 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
    29161 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
    29221 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
    29281 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
    29341 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
    29401 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
    29461 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
    29521 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
    29581 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
    29641 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
    29701 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
    29761 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
    29821 ggaaataata tcttaaataa atgccaaacg gtatgagtaa taaaacatta aaagctggtt
    29881 ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac aagaccaata
    29941 ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc tttggccctg
    30001 tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa tacgcacaca
    30061 ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat gttccatact
    30121 ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat ggaaaaacaa
    30181 tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt gattctttgt
    30241 atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct attatttctc
    30301 atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct gaatatgtac
    30361 tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc ttatgtaaga
    30421 aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg tttcatgcta
    30481 gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat gactccacat
    30541 gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat tttcatttct
    30601 ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca gttacgcaaa
    30661 tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt gctatttcat
    30721 cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca tttcttccac
    30781 gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct aataaaattc
    30841 gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc aagtgtcaat
    30901 actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac gataaaagtc
    30961 cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta tggtcgaaac
    31021 aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt ataaaaaatc
    31081 caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat catcatgcca
    31141 atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt atcatgaagt
    31201 ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata agggagacgc
    31261 attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat accaactgtg
    31321 accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc cgcattgctg
    31381 tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac taataaataa
    31441 acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt cgccggcttt
    31501 ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa catcgttgct
    31561 agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg gatcggaaac
    31621 agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac aaacaaagga
    31681 ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga acctaaaaaa
    31741 ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat tgactgggaa
    31801 agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc tacatttgct
    31861 ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt tccgtaaatc
    31921 cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag ttcagctcct
    31981 gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat atgcaaactg
    32041 gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc accgatcact
    32101 gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa ttcattttct
    32161 atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc cctttcatac
    32221 catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt tataccgtct
    32281 ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct aagtcgtatt
    32341 tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt ggccaagatt
    32401 ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct ggtatgattt
    32461 tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata ttgaaggcat
    32521 ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca tcgatcgcat
    32581 ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg tactttcctt
    32641 ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct cctggcaagg
    32701 ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca tatttcacat
    32761 aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg gttgtaggac
    32821 gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac gcgccgattg
    32881 aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca ttgaccagcc
    32941 cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct gacaaaagag
    33001 cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc attttttcgt
    33061 aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca ggcatgcgta
    33121 tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg atcaactctg
    33181 aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc ctccctgatc
    33241 ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc tctaatttta
    33301 aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta ttttttgata
    33361 aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca tatttctgat
    33421 cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa tcccatgttc
    33481 gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga ctggagtaat
    33541 aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt taaaataatt
    33601 agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag taatcgaagc
    33661 caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg gccgacttct
    33721 agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa caacacctat
    33781 agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa aatgcttgac
    33841 ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt aggtgatcag
    33901 tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc catccatgga
    33961 gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta tcgcttcctt
    34021 tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt ttgctccgga
    34081 taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga tagcatcttc
    34141 ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt gaaaggcgat
    34201 tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat ggattttcag
    34261 cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg ttgcttcaat
    34321 acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg atgctagatt
    34381 gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta gtatgtcgat
    34441 cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat acattgttag
    34501 ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac ataccaaaca
    34561 tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg cttacactga
    34621 ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt caaatgactt
    34681 atatagtatg aacaattacc ttttagataa tgatctattt tactctacct gggcagataa
    34741 atacaacaca accattgata gaagcccttt cgttaaaatc aattgtattc tgaatattta
    34801 atttatttat tgcatagacc aataaaaata cataaaattt ccatcttcgt ttatcagcaa
    34861 tttttaattt tactctctga atatttctta aaagttttac ttgttgaatt cgaaaaaagg
    34921 actgtgacaa aagtcttgtc acagtcctat tcttccgaat aa
//
LOCUS       DASJST010000009        34428 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJST010000009 DASJST010000000
VERSION     DASJST010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.009"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            378..1067
                     /locus_tag="U4B05_000343"
     CDS             378..1067
                     /locus_tag="U4B05_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2049508.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1054..2262
                     /locus_tag="U4B05_000344"
     CDS             1054..2262
                     /locus_tag="U4B05_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2049509.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2556..3827
                     /gene="serS"
                     /locus_tag="U4B05_000345"
     CDS             2556..3827
                     /gene="serS"
                     /locus_tag="U4B05_000345"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN2049510.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4376..5860)
                     /gene="guaB"
                     /locus_tag="U4B05_000346"
     CDS             complement(4376..5860)
                     /gene="guaB"
                     /locus_tag="U4B05_000346"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN2049511.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6365..7060)
                     /locus_tag="U4B05_000347"
     CDS             complement(6365..7060)
                     /locus_tag="U4B05_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN2049512.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7150..8250)
                     /gene="ychF"
                     /locus_tag="U4B05_000348"
     CDS             complement(7150..8250)
                     /gene="ychF"
                     /locus_tag="U4B05_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN2049513.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8259..8450)
                     /locus_tag="U4B05_000349"
     CDS             complement(8259..8450)
                     /locus_tag="U4B05_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN2049514.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8471..9361)
                     /locus_tag="U4B05_000350"
     CDS             complement(8471..9361)
                     /locus_tag="U4B05_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN2049515.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9348..10115)
                     /locus_tag="U4B05_000351"
     CDS             complement(9348..10115)
                     /locus_tag="U4B05_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2049516.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10127..10840)
                     /gene="rsmG"
                     /locus_tag="U4B05_000352"
     CDS             complement(10127..10840)
                     /gene="rsmG"
                     /locus_tag="U4B05_000352"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN2049517.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11072..12973)
                     /gene="mnmG"
                     /locus_tag="U4B05_000353"
     CDS             complement(11072..12973)
                     /gene="mnmG"
                     /locus_tag="U4B05_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN2049518.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12990..14387)
                     /gene="mnmE"
                     /locus_tag="U4B05_000354"
     CDS             complement(12990..14387)
                     /gene="mnmE"
                     /locus_tag="U4B05_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN2049519.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14686..15069
                     /locus_tag="U4B05_000355"
     CDS             14686..15069
                     /locus_tag="U4B05_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049520.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15237..15995)
                     /locus_tag="U4B05_000356"
     CDS             complement(15237..15995)
                     /locus_tag="U4B05_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN2049521.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16013..16831)
                     /locus_tag="U4B05_000357"
     CDS             complement(16013..16831)
                     /locus_tag="U4B05_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2049522.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16828..17184)
                     /gene="rnpA"
                     /locus_tag="U4B05_000358"
     CDS             complement(16828..17184)
                     /gene="rnpA"
                     /locus_tag="U4B05_000358"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN2049523.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17302..17436)
                     /gene="rpmH"
                     /locus_tag="U4B05_000359"
     CDS             complement(17302..17436)
                     /gene="rpmH"
                     /locus_tag="U4B05_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN2049524.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17960..19294
                     /gene="dnaA"
                     /locus_tag="U4B05_000360"
     CDS             17960..19294
                     /gene="dnaA"
                     /locus_tag="U4B05_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN2049525.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19492..20622
                     /gene="dnaN"
                     /locus_tag="U4B05_000361"
     CDS             19492..20622
                     /gene="dnaN"
                     /locus_tag="U4B05_000361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN2049526.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20851..21093
                     /gene="yaaA"
                     /locus_tag="U4B05_000362"
     CDS             20851..21093
                     /gene="yaaA"
                     /locus_tag="U4B05_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN2049527.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21080..22204
                     /gene="recF"
                     /locus_tag="U4B05_000363"
     CDS             21080..22204
                     /gene="recF"
                     /locus_tag="U4B05_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN2049528.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22201..24147
                     /gene="gyrB"
                     /locus_tag="U4B05_000364"
     CDS             22201..24147
                     /gene="gyrB"
                     /locus_tag="U4B05_000364"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN2049529.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24167..26638
                     /gene="gyrA"
                     /locus_tag="U4B05_000365"
     CDS             24167..26638
                     /gene="gyrA"
                     /locus_tag="U4B05_000365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN2049530.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26874..27173
                     /locus_tag="U4B05_000366"
     CDS             26874..27173
                     /locus_tag="U4B05_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN2049531.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27223..27735
                     /gene="ssb"
                     /locus_tag="U4B05_000367"
     CDS             27223..27735
                     /gene="ssb"
                     /locus_tag="U4B05_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2049532.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27761..27997
                     /locus_tag="U4B05_000368"
     CDS             27761..27997
                     /locus_tag="U4B05_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN2049533.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28140..30113
                     /locus_tag="U4B05_000369"
     CDS             28140..30113
                     /locus_tag="U4B05_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN2049534.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30119..30571
                     /gene="rplI"
                     /locus_tag="U4B05_000370"
     CDS             30119..30571
                     /gene="rplI"
                     /locus_tag="U4B05_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN2049535.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30845..32212
                     /gene="dnaB"
                     /locus_tag="U4B05_000371"
     CDS             30845..32212
                     /gene="dnaB"
                     /locus_tag="U4B05_000371"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN2049536.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32992..34284
                     /locus_tag="U4B05_000372"
     CDS             32992..34284
                     /locus_tag="U4B05_000372"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN2049537.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10497 a         6691 c         6817 g        10423 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcatttc caacatgtca acaacttatt ttttattatt
       61 taataagtta ttggtgttct tttgaacaaa taataatata tcaacatcac atgtgaaagt
      121 caataagaaa atgcttattt atcaacattt ctagcttaca tgatcattgg ttagttctgg
      181 tcctttttcg ttcgtcttta taactccgag agacgtaaaa tccttttgtt ttcagtttaa
      241 tgttcatgtt cttgtcttat tcacaccaac tagatgtctc accttctaaa attttcctta
      301 acaaagtgct gcttttatct atttctgctt ttttttgcta gaataatagt aacaatatga
      361 ctctggagga tttatttatg aaaattttga ttgctgacga tgataaagaa atcgttgaat
      421 tattaagtat ctatgttcac aatgaggggt atgaagcagt aaaggcttat gatgggaaag
      481 aagcattatc taaaatcaga acaatccctg atattgaatt acttatttta gatatcatga
      541 tgcctgagat ggatgggatg caagtagtga aagagctacg aaaagaatcc caaataccaa
      601 tcattatgct gacagcaaaa actactgata tggataagat caaaggactg gtcgctggtg
      661 cagatgatta tgtgaccaag ccatttaatc cgttagaagt aatggctaga gtaaagtctt
      721 tattacggcg tacaaaaatg cagattgccc cagatcagcc tgatgtatta gaagtaaatg
      781 cattaatcat caataaagac tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt
      841 taacagcttt ggaattcggt atcctctact tgcttgctag tcatcctaat cgagtattta
      901 gcgcggatga aatttttgag cgtgtttgga aacaagaaag tattgtctct gctaaaactg
      961 tcatggttca cgttagtcat ttaagagata agattgaaga agcaactggt ggtgaaaaag
     1021 tcattcaaac cgtttgggga gtgggctata aaattgacgc aagatagtaa acgagcaact
     1081 gaaaaacgcc gtcggatcaa tttgacttca aaagaaatca gcgaattgtt agcggaagga
     1141 atcatcacga ttatcctttt gcttctatta aatgtttcta ttctggttgt tatcagttcg
     1201 gtgatcaaca gttcaccttc tttgacgaat gcgatttggg attcaaagaa tatttttgca
     1261 gaacgattga acactgattt gttctggaac ggccggaatt ttattattcc ctttttcttt
     1321 ctattagata ttggtgtttt gtattggcgc ttgattcgaa gatatcgcca aatgcaattg
     1381 cggcatatca tcagcgaact tcactacatc gcaaacggaa attatgatca tcgtatccct
     1441 tttgaactga gtggtgactt aagccgagtg gtaacaagta tcaatggatt ggtggatagt
     1501 accgtagcag cgattgaaga cgaacgtaaa atcgaaaaat caaaagatga attgatcacg
     1561 aatgtcagtc atgatatccg taccccactc acgtccatca ttggatacct aggactgata
     1621 gaagatggcc agtatcactc agaagaagat cttttaaagt atacgcatac tgcttatatc
     1681 aaagcaaagc agatgaaatc tttagtggac gacttatttg aatacactaa ggtacgccag
     1741 cctgctgtcc cggtgaattt ttctgccttt gacatgatcc agttgatcga acagttagca
     1801 gctgattttg agttggaagc ttctaaaaaa aatatccaaa tacttgttca atcaaaagta
     1861 gactccctta tcatggatgg cgataccgaa aaacttgtac gtgtcttcaa taatcttttg
     1921 acgaatgcgt tgaaatacgg aaaaggagca acgaagatcg taatcgaagt agaacgtatc
     1981 ggttcagaag ttgttgcaac agtcaaaaat aacggagcaa tgatcccaca acaggcaatc
     2041 gataatctat tcgaccgttt ttatcgtgta gaagaatctc gttcacaagc aactggagga
     2101 actggacttg gtttagcaat cgctcaaagc attgtggctt tgcacggcgg atacatttat
     2161 gcaaaatcag ataagcaatg gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa
     2221 aaattaccta ttgatacaca ggaagttatt gacgaaagct gaaatattcg ctattcttat
     2281 tacaattatt agaataaatg aaaaacacag aacccagtac actttattct ttttcagaga
     2341 gctgatggca ggtgtaaatc agcaaaggat aatttggaag ttccagttcg tttacgtgcc
     2401 ctttcgaaat aagaaaggtc ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg
     2461 ctgaacttgg gtggtaccgc gaatttcaga ctgattcgtc ccaagaataa tgggatggat
     2521 cagtcttttt ttattttcaa aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac
     2581 aaaatattga agaagtacaa gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag
     2641 ctgaattcat ggaactagat gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa
     2701 aaaaatatcg aaatgatgtt tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg
     2761 cttctgctaa aatcgctgaa atgaaagaag tcggtggcaa catcaaagca ttggatactg
     2821 aaattgctga agtggatgaa aaattgaaac aaattgcaac tactttgcca aaccttcccc
     2881 atgattctgt gccagttgga acagatgaag aggacaatgt agaaatccgt cgttggagcg
     2941 aaccacgtac atttgctttt gaaccaaaac cacactggga aattgcagaa aatcttgata
     3001 ttttagattt tgaacgtggc gcaaaagtag ccggtagccg ttttgtctat tacaaaggat
     3061 taggtgctcg attagaacgt gctgtctaca acttcatgct tgatcagcat atttacgaac
     3121 acggctatac tgaaatgatc actccttata tcgtcaacag taaagcgatg tttggtacgg
     3181 gacaatttcc aaaattcaaa gaagatgttt tccaattgga gaatactgat ctaacattaa
     3241 tcccaacagc tgaagtacca ttaactaact attataatga tgaaatttta gatggtgcag
     3301 atttacctat ttatttcact gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg
     3361 gtcgggatac acgaggactg attcgtcttc accaattcaa taaagtagaa atggtcaaat
     3421 tttcggatgt agagcattct tacgaagaat tggaaaaaat gacagctgat gcagaagata
     3481 tcttgaaaaa attgcatttg ccttatcgtg tgatggcctt atcaactgga gacatgggct
     3541 tttctgcagc taaaacttat gacttagaag tatggatacc tgcacaagat acttatcgtg
     3601 aaatcagttc ttgttctaac tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc
     3661 gcgataatga tggaaaaatc cactatactc atactctgaa tggttcagga cttgcagttg
     3721 gccgtacagt agctgccatt ttggaaaact accaaaatga agatggttct gtcacagttc
     3781 cagaagcgct cgttccttat atggggaatc tgaaagttat taaataaaga ggctgtgaaa
     3841 aagccgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
     3901 ataaattgga cttaataccg agaaactggc ttttgaacac catttatcga gaggttgtga
     3961 aatcagcgaa ttaacctgag tattagagaa aaaaatggaa aaatagtccc tcatatttta
     4021 ccgtttgttg aactaatacc gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt
     4081 aagcctaccc cccagctcct agttacaagg acaaattcaa aaaacagctc ttcatatttt
     4141 atgaatgttg tacttgtgat tcggaggtgg caagggaaca ctgtttattc aaaaagaggc
     4201 tgggacagaa gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa
     4261 tttttgtgaa tttcttctta tttccgaagg agttgcttct acttccgccg tttatacgtg
     4321 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctctttaa tgtttttaag
     4381 attcaactga atagtttggt gcttcttttg tgatttgtac atcatgaggg tgtgattctt
     4441 ttaatccact gccgctcatt tggataaatt gcgcttcatc acgtaattgc tgtagattgg
     4501 ctgccccaac ataacccatt cctgaacgca aaccgccgat catttggaaa ataatatctg
     4561 caacacttcc tttataagca acccgacctt cgattccttc tggtactaat ttatttgctt
     4621 cattgacact accttggaag taacggtctt ttgaaccttt ttccatcgcg cctaatgagc
     4681 ccatacctcg gtatgttttg aatcgacgtc cttggaagat ttcgaattca cctggtgatt
     4741 catctgtccc tgccaacata cttcctagca tcactgcatg tccgccggca gctaatgctt
     4801 tgacgatgtc accagaatat ttgatccctc cgtctgcaat aatcgctttg ccatattgac
     4861 gagcaactga tgctgcatca taaatagctg tgagctgagg tacacctaca ccggctacca
     4921 cacgtgtcgt acaaatcgat cctggtccga taccaacttt tacgacatct acacctgcat
     4981 catataaagc ttttgtcgct tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg
     5041 caaaagttga acggatttct tgaatttttc ggatcacacc tgcactatgt ccatgagctg
     5101 tatcgatgat gattgcatct gcccctgctt ctaataaagc attcgcacgc tcaaatgtat
     5161 cgcttgttac accaacggct gctgctacaa gcaatcgacc atgttcatct tttgctgcat
     5221 ttggaaattc gatcactttc tcgatgtctt tgatcgtgat caatccgctt aaacgacctt
     5281 cgttatctac gattggtaat ttttcgattt tatgtttttg caaaatcttt tctgcatctt
     5341 ttagagaggt acctactggg gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg
     5401 gcatgtgata atctgttaca aaacgcatgt cgcggtttgt aattatccct accaattttc
     5461 tgttttcaag tgtttcaacg attggcacac cactgatacg atatttgctc atcaattctt
     5521 ctgcgtctgc tacaagatgc tgcggcgtta agaagaacgg atcaatgatt acgccacttt
     5581 cagaacgttt gacttttctt acttcatccg cttgctggct gatcgtcatg tttttatgga
     5641 tcacacctag tccgccttgt cttgccatag caatggccat cttgctgtcg gtaacagtat
     5701 ccatacttgc actgatgatt ggaatattca acttaatatt tttagctaat tgtacgctca
     5761 tatccacttc gtttggtaac acgtgacttt ctgctggtat caataagaca tcatcaaaag
     5821 taaatccttt tttcgtgaac tttgtttccc agttggacat ttttagtacc actcctctaa
     5881 tttagtttat ctattaaaat aattgatttt ttcatttaag tcaatgatct acttggaatc
     5941 aagttagctt tctcatagaa taccaatata aagcgtttac atctgtgtgt attaaaagag
     6001 attgtaaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa cagcttctca
     6061 tgtttttgaa ttggttgggt ttattaccgc agatgcaaac ttttgaacac cgtttagtgg
     6121 agaggctgtg aaaaagctgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt
     6181 tccacatttt tgcataaatt ggacttaata ccgagaaact ggcttttgaa caccgtttat
     6241 cgtaaaaagg gactgtgaca agacttttgt cacagtccct tactattatt taactatgga
     6301 taaacaaaat gaggttcttt actaccccta gatatctagc aaaatagctg atctttttgc
     6361 tagtttatct tcttgggacg gataaacttc caataatccc gtattttttc ttcaagtagt
     6421 aacggattgc taaagcagtt gccccgataa tgataactac tactggatct aatactggat
     6481 tgataactgg tggcaatagt gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc
     6541 cacctaggat aagcaatgtc ttacccattc ctggtttctt gcttcgatct gctccgggat
     6601 attcatattg atagatgtat ttgtacatca tataaaagac ccagccaccg attgcagaac
     6661 ctagaacgag tgtagaaaag ccataagctg gcgttgttcc acgtgaaaac aatccggtaa
     6721 tcgcaatcat gatacttaat agaccaagca acagcaacgt attatctaac cacatcaata
     6781 aaggggtgga tgaatttttt acttctgctg gcttattgat gattgcttcc gttctttcag
     6841 agactgtccc aaacaattga cgggctgttc ttccgccttt ttgttctgca actaaagtag
     6901 gcagaatgtc atgaagggct tttacttttt cttcttcaga caaattcgct gcttctaaag
     6961 attttttcaa atcaaaaatg tactgttgat ttcttttggt taattttgtt tctaaactat
     7021 tgttttcttt tacgattgct cgtaattgtt ctggttccat tatctaatag tcctccttag
     7081 ccgttccttt tctcaacggt cgtcacaaaa gacattctac catattctac agctagtttg
     7141 atagcccttt tacacattaa agcggaataa cattacgtcg ccgtcttgta cgacataatc
     7201 ttttccttct aatcgaacac gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc
     7261 caagtcttca aaagagacag tttctgcacg gataaatccg cgttcgaaat ctgaatggat
     7321 gattcctgct gcttgtggtg ctttgattcc ttttttgaat gtccaagcac gtacttcttg
     7381 ttccccagca gtaaagtaag tagctaagcc taacaaatca taagcagcac ggatcaactg
     7441 atccaaacct gattcttcga ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc
     7501 taattctgcg atttcttctt ctgcacgagc actgaccacg atcacttctg cattttcttc
     7561 tgcagcaaag ttgcgcactt cttgtacata atgattcgcg tctgcatctg ctacgtcatc
     7621 ttctgagaca ttggctacat aaagaattgg ttttgtcgtc aataaaaata gtgatttaac
     7681 gattttttgt tcttcttcag taaattcgat cgttcgggca gattttcctt cttccagtac
     7741 aggtttgatt ttatccagta cagctagttc tgccactgca tctttatctt tggttttagc
     7801 aacttttgct acacgagtat agcgtttgtt gatagattct aagtctgcta acactagttc
     7861 caagttgatc gtatcaatat cttctagtgg atcaacacgt ccttctacgt gagtaatatt
     7921 atcatcatca aaacagcgaa caacgtgaca aatagcgtct acttggcgga tatggcttaa
     7981 gaattgattt cccaaacctt ctcctttact tgcgcctttt acgattccag caatatctgt
     8041 aaattcaaaa gtcgtcggaa cagttttttt cggatgtaca agttctgtta agcgttgcaa
     8101 acgccagtca ggtacttcta ccatccctac gttggggtcg atcgtcgcaa agggatagtt
     8161 tgctgcttct gctcctgctt ttgtgatcgc attaaacaaa gttgattttc ctacgttggg
     8221 caacccaaca attccagcag tcaatgccat aaatttattc actctttctc tgcatttttt
     8281 tcaattactt tttttaattt cttttcaaaa tctcgacggg tcatcatgac gatatgtccg
     8341 caattcatac atctgatttt gatatctgcg cccattcgaa tgacttccca gcgattcgtc
     8401 tggcaagcgt gaggtttttt catttcaacg atatcgtgca agtcgtacat aaaaatagcc
     8461 tcctttttgt ttattcttca tcaaattgaa tattcaagat atcaagaatt cgagtcaaat
     8521 ctttttgaga aagatattca atctcgatct tacctttccc atttttttct tggatagcta
     8581 cacttgtacc aaatttatcc atcaaacgat cttcgctttc tcggatataa tatggttttt
     8641 cctttgctag ccttgggatt tttttctttt cttttccatc attttgattt aactcagaaa
     8701 caagctgttc cagttggcgg acagttaaat tttcagttac tgcacgattg gctaatttca
     8761 agatcaatgt tttatttttt aaacctaaca gcgtacgggc ttgtcccata gataaacgct
     8821 gatcttgtac catttccttg acaagatccg gtaaagaaag caagcgcaaa taattggcga
     8881 tataaggtct gctctttcct aaacgactag ctacttctgc ttgtgttagt ttcaggtttt
     8941 tcatcagcat ctcatatgct tctgcttctt ctaacggatt cagatcttct cgctgcaagt
     9001 tttccagcac tgctacttgc atcatcgctt cttcgtcaaa gtctctaacg atagcaggga
     9061 tcgtttcttt ttcagctagt ttggacgctc gaaaacgtcg ctcccctgca ataatctcat
     9121 atccttttac tgcggaacgg cgtacgatga tgggttgaaa gacacctgat tgttcaatag
     9181 aattagctag ttcctgcaaa gatacttctt caaatgtttt acggggctga tacggatttg
     9241 gtcgcagctc attcaatggt agctggacaa cggtatcatc ttttacatcc accgtctcta
     9301 agttttctaa atcttgaaac agtgcatcga ttcctcggcc taatccttta cttttcttca
     9361 cgacttacca cttcctttgc caatgcttgg tagacttcag ctcctcgaga acgtggatcg
     9421 tagtcgatga tcggttttcc atgactaggt gcttctgata aacgaatatt tcttggaatg
     9481 atcgtttcgt aaactttttc gcggaagtat ttacgtactt cttctacgac ttcatttccc
     9541 agatttgttc gtgcatcata cattgtcagt aagactccct cgatctctaa ttctggatta
     9601 aagtgttttt ggactaaacg aaccgtattc agtaattggc ttaatccttc caatgcgtaa
     9661 tactcacact gaacaggtat caaaatcgaa tcacttgctg taaatgaatt gattgtcaga
     9721 tggccaagag aaggcgggca atcgatcaaa atataatcat actgactgct tacttctgct
     9781 aaagatcctt ttaatcttga ttctcttgcc atcattgacg tcaattcgat ctctgctcct
     9841 gctaattgga gagtcgcagg cacaatactc aaattttcgt gttcagtgat caaagttgcc
     9901 tcatcaattg gcaattcatt gactagaaca tcatagatgt ctcttgtcac atcaggtttt
     9961 ctgatcccga caccactggt agcatttcct tgtgcatcca tatccactag caatactttt
    10021 ttgccaaggc tagcaagaca agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca
    10081 cctttttggt tcgcaacaga tattatttgt gccatctctt ctcctcctat ttgattggct
    10141 gcttattagg cagtccgggc tttcttgggt atttctttgg ggttggtttc tttttttgaa
    10201 taacgatgat atgccgttga tcagagacat tcggcaaggt aaccgcctct tctttgagga
    10261 atttaccacc taataaagca atcgcttgtt ttgcttctgt caattcctct tcactttttg
    10321 ctgctttcaa tgcataaaaa tagccttctt gtttagccaa gggcaaacac aattccgcta
    10381 acacattcaa acgagcaacc gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag
    10441 gattttgccc aaatgtttct gcccggtcat gatataagga agcatcgatt cctaataatc
    10501 gggttaactc agataagaag gttattcgtt tattcagaga atcaacgatc gttatctgca
    10561 gttctggaaa ggcaattttt aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc
    10621 ataaagataa aggttgcgta aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat
    10681 gcttcagata tacgtctttt tcatccgtaa tagctgtaag gttcatcttt tcgttccatt
    10741 cgactaatgt ccgataataa atagcgaatt gattcatttg atgatcggta agctctatcc
    10801 ctttttctgc caaggattgg cgaaattctt ctggtatcat aaatattcct ttcattgtga
    10861 aacagcaatt gtttcacaga ttctctccct actttaacgc aaaatctcta gacatgcaat
    10921 ccttccatat ggattcttaa aaatttgaaa agtaagaaaa gccgatagac tttttctttc
    10981 tagtatgact cataaaatat acacagaaga gagaactagc tttgagcaat ttctgcaaaa
    11041 actagttctc tcttctattt attattacgt attacgctgg ttcactgact ttcgcaatct
    11101 tcccttgttc gatatagacc attaggatac ttaggtctgc agggtttacg ccactgattc
    11161 ggctagcttg ggcaattgtt tctggttgga ttttctgcaa tttttgtttt gcttctgttg
    11221 ctaatccgtt gattgctgcg tagtcgatgt tttctggaat ctttttcgct tccatgcgtt
    11281 ttaatttttc tactttttcc atggcttttt tgatgtagcc ttcatatttg atttggattt
    11341 ctacttgttc gatttctttt cgtgtcaatt cttcattttc tggaataaac cgtagaagtt
    11401 ctgcataagt aatctcagga cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg
    11461 gtgccgcatt aatcgttgct aaaaagtctt ggacttcttc tgttggtttg attcgtacat
    11521 tcatcaaacg tttgatttct tgttcaactg cttctttttt cttcaaataa ttcttgtatt
    11581 gatcttcttt tactaagcct acacggtgac ccatttctgt taatcgcaaa tctgcattat
    11641 catgacgtaa aagcagacgg tattcagcac gtgaagtcag caaacgataa ggttcgttcg
    11701 ttccttttgt taccaaatca tcgatcatga caccgatgta accgtcactt cgtttgatca
    11761 ctaaaggttc ttttccctga actttcaaag ctgcattgat accggcaatc agcccttgtc
    11821 cagccgcttc ttcataaccg cttgtaccat ttgtttgtcc agctgtataa agattttcaa
    11881 cgactttagt ttctaatgtc ggacggagct ggtgtggctc tactacgtca tattcgatag
    11941 catatcctgt acgcatcatt tctacttttt ctaatccagc aatagaatga agcatgttga
    12001 cttgaacatc ctctggtaaa gaagtagata aaccttgaac atagacttct tccgtattta
    12061 agccttctgg ttccaaaaat aattgatgac gtggtttgtc tgcaaaacgc acgatcttat
    12121 cttcgatgga tggacaatag cgagcgccta ccccttcaac gatccctgta aacattgggg
    12181 cacgatgtaa gttgtctcga ataatttggt gagtcgtctc acctgtatag gtcagccagc
    12241 aagattcctg atctagttta taggcactat ctggtgtgct gaagctaaaa tgattcggct
    12301 ctttgtcacc aggctgctct tctgtaacag aatagtcgat cgtacttgat tttactcgag
    12361 gcggtgttcc tgttttgaac cgttcgatag caaagccaag ttcttttaga tgattcgcta
    12421 gtccaataga aggttgagag ttattcggac ctgatgaata tttcaattca ccgataatga
    12481 tttctccgcg aagtgctgtt ccggctgtaa taacaactgc tttggcccgg tagctcgctc
    12541 ctgtggacgt cactactcca tgacaaacac catcttcgac gatcagttct tcgacgattc
    12601 cttgtcttag cgtcaaattt ggttcttttt cgatcgtatg tttcatttct gttgaataag
    12661 catgtttgtc tgcttgtgct cgcaatgctc ggacagctgg gccttttcct gtgttcaaca
    12721 tacgcatttg gatataggtt ttatcgatat tcttacccat ttctccgcct aacgcatcga
    12781 tttctcgaac gacaacgcct ttagctggtc cgcctacaga aggattacat ggcatgaatg
    12841 ctaccatgtc caaatttatt gtcagtaata atgtttttga tcccatacgt gcagcagcaa
    12901 gtgcagcttc tgaaccggcg tgtcctgcgc caacaacgat cacgtcatac gcatctgctt
    12961 cataatgttc caatcgtttg ttcctccttt tattttccta aacaaaattg actgaataat
    13021 tgagtgatta gctcatcttg tacactgtcg ccgacaactt ctcctagata gtcccagcaa
    13081 cgcgtcatat ctatttgaac gagatccaca ggcattcctg aatcaatacc tgcaatgact
    13141 tcttcaagtg agagggaagc tttttctaac aacgcgatat ggcgggtatt agaaacataa
    13201 gtagcgtctc tttctcctgt ttctccgctg aagaataaat cagaaatagc tgattccaaa
    13261 cgatctagtc cgtcgttttt agctacggaa attgaaaaaa ctggttcatt ttcgatcaat
    13321 ttcttcaatt tttcttgttc cagctgtgct ggcaaatctg ttttatttag aagaatgatc
    13381 cgtttcaatc cacttgtcgc ttccaataat tgttcatctt ctgctgtcag tggttcgctt
    13441 tggtttagaa caagtaagat caagtctgct tctgctaatg cttttcggct tctttcgacc
    13501 ccgatccgtt ccaccacatc ttctgtttcc cgaattccag ctgtatcgat tagcttgagc
    13561 ggtacccccc gaacattgac atactcttcg attacatctc gagtcgttcc tgcaatatcc
    13621 gtaacgattg ctttttcttc tcttaacaaa tggtttaaca aacttgattt gccgacattt
    13681 ggccggccga tgattgccgt acttaaccct tcccgcagaa ccttcccttg cttggatgta
    13741 gcaagtagcg cttggattct ttgttggatc atctgcgctt tttcaagcaa taacttcgta
    13801 gtcaattctt cgacatcatc atattctgga taatcaatat ttacttctac ttgtgctaat
    13861 gtctctaaaa tctcttgtct caatgagcgg atcaaagcag acagattgcc gtctaactga
    13921 ttaagagcta gtcccattgc tttgtctgtt tttgcccgaa tcaaatccat cacagcttct
    13981 gcctgtgaaa ggtcaacacg accattcaag aatgctcgtt ttgtgaattc gcctggctca
    14041 gctaatcttg caccttctct taacaatagc tgcaaaattt gattgactac aacgatccca
    14101 ccgtgacaat tgatttctac tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg
    14161 gagaccatga cttcatcgac aagctgattg ctcttgggat caacgatatg tccataatgg
    14221 atcgtatgac ttggtacttc tgataatcgt ttgtttccag attgataaac tttatctgct
    14281 agttgcacag cttgatcgcc acttaaacga acgatactga ttgcaccttc tccaggtggg
    14341 gtagaaattg ccgcaatcgt atcaaattct aaagtaattt cagccatttt ttcacttcct
    14401 ttttcatttt gcgcacaaaa aaagtgccca ctccaccctt tatgcacaat aaacggtgaa
    14461 cttcgcactt tgactgcttg ttcttgatta aattctggtc atagattatc acacttgcta
    14521 taaggaatca agtgtttaaa acaattagat gaagttgagc ctgttctgct tccattacct
    14581 tatatttaat tgtccttgga tgtctctttg cgttctttgg tttattcgaa gagaagatac
    14641 tgtataaaca aggctttatc tttcttaaaa aaaacggtac actaaatggt agcgctatct
    14701 aaactataca cttttgaaag gagaaaatat gtgagcccgt tcagatctgc tgcattggaa
    14761 gaagaaagtg accctcggat caacgatgtc gttttatcta cagggaatat caaccagaaa
    14821 tatatcgtcc gcgatatcgt ttttgttgtg gaatgcttag aagctgattt gtttgaccca
    14881 tcttttccca aagatgacct tttcttaaca acgaagagga aattaaagac gaaggtactt
    14941 gaatatggtg cgaatgcagt aatcaactgt cattttgagt atcagcaaaa aaaacgcgac
    15001 ggaaagtatt ttctggaact ttttgcttat ggaacagtga tccaatttat ccaaacaacg
    15061 attggttaaa tgaaacagac aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct
    15121 tttttctatc aaagttagtg actacttcat cgtatactct gataagaatg atttctagta
    15181 aaatatactt ggtttgaaaa actttgtcaa cgtgatttat tcaaaaagta atggaatcaa
    15241 taatattttt tagcaggttc tactactaaa taacgataag gttcctcacc ttccgaatgt
    15301 gtttttacat gttcatcttt gcttaatgca gcatggattt gtttacgttc aaatgcaggc
    15361 attggttcta agaaaacagg ctgccctgtc cgttttactt tttcagccgt gcgatcagct
    15421 aaacgctgaa tgatctcttg gcgcttttca cggtaattgc ctacattgac tactacagac
    15481 agcttatttc ttgctaaacg atggatgaag acttgtgcca gatattgcaa tgcattcagt
    15541 gtttttccat gtttcccaat cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc
    15601 atattatttg attctctttc cattttgaca agagctggag catcaagctg ttttgaaatt
    15661 tcagtaagat aaactgccaa ttccgttaaa gctgcttcat cacctagctc acttacttct
    15721 tctgtttttt cttcaagaat cgtttcgttg actacctcga tctcagctgt tggttcttct
    15781 tccaaaattt cttctacagt ctgttctact gtttcagaaa ctttttcgct gatacttggc
    15841 tggatcgaaa gccgtgcatt ctttcgaccc attcctagga aacctttttt tccttcttcc
    15901 agtatttcga tttcgacttg gtctttggcc aaacccaaat cagccagacc tagttggaca
    15961 gcttcgtcta ttgttgcagc ttcataaatc ggcattagca ttacctcccc tattatttcc
    16021 gttttttctt aggattcatt gctttcttaa gagcacgttc tctttcgcgg attctccgtg
    16081 cttcttcttc acgctctttt cgaattttga aagggttgtt gatcagcagc gtttgtacaa
    16141 cttggaaggc attggaaact acccagtata atgaaagacc gctggctaaa ttgacaccca
    16201 tcactaaaat cattacaggc atcacgaaat tcatgatctt cattgatgca ttcgattcaa
    16261 tttgactcat gctagataaa tacgtacttg caaaagtaaa gattgctgct aaaatcggca
    16321 aaataaatgt tggatccgcg ttccctaagt tcaaccataa gaaatgccct tgtttcaact
    16381 ctggtacacg tgaaatagat tgccataacg ccatcaagat tggcatttgt actaataaag
    16441 gaagacaacc agcatatgga ttgacgccat tttctgcata taaacgctgc tgctcttctc
    16501 ttaacttact ttgtgtttcc ggatctcttg aactatattg ttgctgcagt gcttttaatt
    16561 tcggttgtaa ttcttgcgtt ttacgcatgc tctttgtttg gaaatgcatc aacggcaata
    16621 ggatcacgcg gatgatcagt gtaaataaaa tgatccctat ccctgcatta ccaaatgaca
    16681 acgccttgat cgcctcagca aagtaataaa caatataacg atcccaaatt cctgtactct
    16741 gtgcgctgac ttccccagtt ccgcaaccgg ataagacgat gaccaaacta acgataccgg
    16801 ccatcaaaag taaacgtttg tatttcttca cttactcttt tcctctctgt cgattatttt
    16861 tgcaagtttt aagacgtgga ctaaattgga gcgaacctca tcataagtca accccttcac
    16921 acttggtcta gcaatcacaa taaaatccaa ctccggatca atttcttctt ttatagaata
    16981 aactgctgct cggatttttc gtttgacttc attgcggttt accgcgttgc cgaccttttt
    17041 tcctacagat aaaccaactc gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac
    17101 gaatttgcgg ttagcaaaag aatctcccgt atggaacacg gcttgaaact ctcgttcttt
    17161 ttttactcga tacgcttttc tcattagttt tctttccttt tctatctctc acttacgctc
    17221 ataatcatac cataattacg attgtccttt catcatttcc ctaaaaaaga gtataaaaaa
    17281 aaccactgaa accccagtgg cctaagctga aagaactttt cttcctttgc gacgacggct
    17341 agctaacaca cgacgtccat ttttagtact catacgttta cggaatccgt gaactttttg
    17401 acgtttacgt ttatttggtt ggtatgttct tttcatatat tccacctcca aaatgttctt
    17461 atctatttta taaacacaga catactttga tagtataacg acaaagtagt gattttgtca
    17521 atataaaata gaagatcgat tgttaaaact tatccacaag attatttttt tctatgggag
    17581 tcctatgtgg aaaaagttat gcacagggat tatccacaga taataaatgg ctggttttac
    17641 acagaaaaca cacgtgtaaa aaaagtaatc cacaatcagg tgatttcctg tgtattccgt
    17701 ctcctaacta ttggtatatc tatatttttt atccacagat gcaagaggaa atctattgat
    17761 ttttacgagt tttccacctg tgcattagtg gtgtggaaaa cttttttttg acaactgtgt
    17821 ttttctcttt tttctttttg tcgattgtgt ggataattgt aattaaaaaa tttttttctt
    17881 cggtttctgt ggaaaactat tgaaaaaaat gctaaattag tactagttat tcacttttta
    17941 atgaaggagg aggccttata tggtatccct cgatgcttta tggaatgaat taaaagcaac
    18001 ataccaaaag gatttatcgc ctgctagcta taatacatgg atcgaaacag cgaaccctcg
    18061 aacccttgat caaaaccagc tcgttgttga ggttcctagc aagattcata aagaatattg
    18121 ggaaaaaaac ctggcgacaa aaatcgttga agtcggttat atgttatcag gaaatgagat
    18181 tatccctcgc tttattactg gcgaagaagc agagcaagaa gaagttatag aagaaaaaaa
    18241 tccaaaagtt gtggcaccta gtccgctaaa aaaagccatg ctgaacccta aatatacctt
    18301 tgatacgttt gtcattggta aaggaaacca gatggctcat gcagcagcgc tcgttgtagc
    18361 agaagatcca ggttctattt ataacccgct cttcttttat ggaggcgtgg gacttggtaa
    18421 gactcacttg atgcatgcaa ttggtcatca aatgctgcaa agtcagccta atgccaaagt
    18481 aaaatatgta agcagtgaaa cttttgctaa tgatttcatc aattcgatcc aaaacaaaac
    18541 agcagaagag ttccgtcaag aatatcgaaa tgttgattta ctgctagtgg atgacattca
    18601 attttttgca gaaaaagaag ctacgcaaga agagtttttc catactttca atgccttgta
    18661 caatgaaggg aaacaaatcg tactaaccag tgatcggttg ccaaacgaga ttccgaaact
    18721 gcaagaacgc ctcgtttctc gctttgcatg ggggctgtct gttgatatta caccgccgga
    18781 tcttgaaaca agaacagcga ttttacgtaa aaaagcggcg gctgaacgtc tagagatccc
    18841 agatgacaca ctaagctaca tcgcaggaca aattgattct aacattcgtg aattagaagg
    18901 tgcgcttgtt cgagtgcaag cttttgctgc gatgaatagt gaagatattt cgactagtct
    18961 agctgcagac gcactgaaaa cgttaaaatc aggaaaagga catcctcaat tgtcaatcct
    19021 gcagatccaa gaagaagtgg caaagtatta tcacatccaa ttgaaagata tcaaaggaaa
    19081 gaaacgcgtc aaatctattg ttgttcctag acaaatcgct atgtatttgg cacgtgagct
    19141 cacagataat tctttaccga aaatcggagc tgaattcggt ggcaaggatc atacaacggt
    19201 tatccatgct catgaaaaaa tccagcagct gatggattct agtgtcagta tgcaaaatga
    19261 agtatcagaa atcaaaaact ttttactaaa ttaatttgtc gattctatca actgaaaata
    19321 gcatgtggat aaaagaaaaa aacgttcaaa agttatgcac actttttcca cagctgtttt
    19381 ttcgtttctc tcttacgttt tttgagtttt ccacagaatc aacatgccct attactttta
    19441 ttattctttt taactaataa taaataaaca aacattcaaa ggagatacat catgaaagtt
    19501 actttaaacc gagctagctt tatgcaggaa ttgcaaactg ttcaacgagc tatttcaagc
    19561 aaaaccacga tccctatttt gacaggtgta aaaatcacac tgacacaaga aggtttgact
    19621 ttgacgggga gcaacgctga tatatcaatt gaaacttttt tgtctgttga aaacgaaaaa
    19681 gcaaatatgc aaatcgaatc tactggttcc attgttttac aagcacgttt ctttagcgaa
    19741 atcattcgga gacttcctga agaaacattt actttagaag ttttagaaaa taaacaagta
    19801 gcgattactt ctggaaaagc gaattttatc gtaaatggat tagatgcaga taactatcct
    19861 catcttcctg ttgtcgaaag ccataaccag atgaaattac ctgtacacgt attgactaaa
    19921 ctaatcaacg aaacagtttt tgctgtctca caacatgaga gtcgtccaat cttgacaggt
    19981 gtgcatttca ttttatctga taattcttta ttggctgtag ctactgattc tcaccgtcta
    20041 agtcaacgcg tgattccagt agaacaagcg gctgatcatt ttgatattgt tattcctgga
    20101 aaaagtttga tcgaattatc tcgctcatta acaaatgaag aagaaatcgt cgaaatcagc
    20161 attatggaaa accaagtgtt gttcaaaaca gaaacgatgt atttctattc ccgtttgcta
    20221 gaaggaaact atcctgatac caatcgtttg attccttcaa gttttaatac agaggttgaa
    20281 ttttctgttc ctagcttttt agcggcgatc gaacgggctt ctttactttc tcatgaaggc
    20341 cgtaacaaca tcgttcgttt atctattcgt ccagatgcgg ttgttttata cggaaattcg
    20401 ccagaaatcg ggaaagtaga agaaagcttg agttatacag caagcagtgg cgatccatta
    20461 gatatttctt tcaatccgga ttatatgaaa gcagcgttgc gtgcatttgg agatatgagc
    20521 atcaaagtga aatttatctc tgctattcgt ccatttacat tggaaccaac ggaagatggc
    20581 gttcaattta ttcagctgat tactcctgta cgtacaaatt aacaatgatt ttcattgttc
    20641 ttgaaaattt aattgaaaag aaatagaaaa aataaaattt taaacagaag gccctaaaat
    20701 ctcttttaag gctttctgtt tatttttgtt taaatcacct tttttagatt aaatcgtctt
    20761 atatggaaaa atatatggat aaatttgttt tattgcgaaa aaaaaggtat aatagagtat
    20821 acttttaata atgaaaatga gggatgggca ttgaaacaac aagtcattct aaatacggaa
    20881 ttcatgacgt tgggccaaat gttgaaagaa gtcacagtta tcggtagtgg cggacaagca
    20941 aaatggtatt tggcagaaaa cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga
    21001 cgcaaacttt atcccggcat gatggttgaa gttccagaag taggaacttt ttttatggta
    21061 aaaaaaggaa acgacgataa tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta
    21121 cgaagagctg aacatcgaat tttccaagaa cctcgtcatc tttttaggag aaaatgcgca
    21181 aggaaaaacg aatcttttgg aaagtatcta cgtattagct atgacgcgaa gtcatcggac
    21241 aagtagtgag caggaattga ttggctggac tgacgatcag gcgatgattc aaggagagat
    21301 cacaaaaggt tcatccactc ttccattaga aatcttatta tctaaaaaag gccgaaaaac
    21361 caagatcaac catatcgaac aaaaaaagtt aagtacttac gtgggacaaa tgaatgtcat
    21421 tttatttgca ccagaagatc tttctttagt aaaaggcagc ccgcagatca ggcggaagtt
    21481 tttagatatg gaattaggcc agatcaatcc aatctatctt tatgatcttg tccaatacca
    21541 agcaattttg aaacaaagaa accagtattt aaagcaattg aatgaaaaaa aacaaactga
    21601 tttgctttat ttggatattt tgtccgaaca actagcagcg tttggcagca aagtattgaa
    21661 agcaagagca cagttcgtca agcgattgga gttttgggct aattctttgc atcagcaaat
    21721 cacccatcaa aaagagcagc tggaaatcga gtatctcacc gcagtcgatt ctttagaaac
    21781 acatacgcaa gagcaaatcc aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa
    21841 agaccttttt cggggaacaa cgacagttgg tcctcatcgt gacgatcttt ccttttttat
    21901 taatcagaaa aatgtgcaga cttatggatc gcaaggacag caaagaacaa ctgcactaag
    21961 tgtcaaatta gcagaaatcg atttgattaa agaagagacg ggggaatatc caatcctttt
    22021 gttagatgat gtgatgagcg agctggatga tagtcgtcaa ctccatctgc tagaaacaat
    22081 cgaaggaaaa gttcaaacgt tcttaacaac aacgacactt gaccatgtaa aaaataaaat
    22141 gactgttgaa cctgagattt tttatgttca acaagggaaa ctaaaaggag aagaaactga
    22201 atgacagaag aaagaagttt agtagaacgc gctaaagagt atgatgccag tcagattcaa
    22261 gtacttgaag gacttgaagc cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt
    22321 tctgagggtc tgcatcacct tgtttgggaa attgtcgata attcgatcga tgaagtatta
    22381 gcaggttttg caacgaaaat ccatgtgatt attgaaaaag acaacagtat caccgttatt
    22441 gacgacggtc gggggatacc tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa
    22501 acggtcttta ctgttttaca tgctggagga aaattcggcg gtggtgggta caaagtttct
    22561 ggtggacttc acggagtagg atcttctgtt gttaacgcgc tttctacaca gcttgatgtc
    22621 aaagtataca aagaaggaaa tgtgtactat caagaatatc gtcgtggagc tgtagtggac
    22681 gacttaaaga ttatcgaaca aacggatcgc catggaacaa cggttcactt tacgccagac
    22741 cctgaaattt ttacagagac aactgagttt gatttttcaa aattggctac ccggatccgt
    22801 gaacttgctt tcttgaaccg cgggatgcgt atatcgattg aagacaaacg agaagaagaa
    22861 ccagtcatca atgaatacca ttatgacggc gggattaaaa gttatgtcga atacttgaat
    22921 gcaaacaaaa ccgtgatttt tccagaacct gtatatttgg aaggtgaaca gcaagatatc
    22981 gcagttgaag tctctatgca atatacagac ggctaccatt ccaatatcat gagttttgcc
    23041 aacaatatcc atacgtatga aggcggaaca catgaatcag gattcaagac tgctttaact
    23101 cgtgtgataa atgattatgc ccgaaaacaa aagctgatga aagaaaatga cgacaacttg
    23161 accggtgaag acgtgcgaga aggattgaca gctgttattt ctatcaagca tcctgaccct
    23221 caattcgaag gacaaacaaa aacaaaattg ggcaattccg aagtacggac agtcactgat
    23281 cggttattta gcgaacattt catgaaattc ttgctggaaa acccaagtgt gggccgtcaa
    23341 attgttgaaa aaggattact agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa
    23401 gtaacgagac gtaaaggtgc tttagagatt agtaatctgc caggaaaatt agcggactgc
    23461 tctagcaacg atcctgaaaa atgcgaattg ttcatcgtcg aaggagattc ggccggcgga
    23521 tcagctaagc aaggtcgtaa tcgagagttt caagccatct tgcctattcg tgggaaaatc
    23581 ttgaatgttg aaaaagcaag catggacaag atcttggcaa atgaagagat ccggtcttta
    23641 tttactgcta tgggtacagg gtttggagaa gattttgatg tatcgaaagc tcgctaccac
    23701 aaattagtta tcatgactga tgccgatgtc gacggcgcgc atatccgtac attgctgctg
    23761 actctttttt atcgttacat gcgtcctatt gttgaagctg gatacgtcta tattgctcag
    23821 ccacctttat acggggtaaa acaagggaag aatattacgt atgtacaacc aggaaaaaat
    23881 gcagaagaag agttgaaaca agtcgttgct tcacttcctg cctcaccaaa accaagcgtt
    23941 cagcgttata aaggacttgg agaaatggat gatcatcaac tatgggaaac aactatggac
    24001 ccaagcaatc ggatgatggc ccgcgtcagt gtggacgatg ctattgcagc agatcagata
    24061 tttgaaatgt tgatggggga tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca
    24121 cactatgtga agaacttaga tatttaatca tgaaggagga aaaagaatga gtgaagaaat
    24181 cagagaaaac atccatgatg tcaatctaac cagcgaaatg aaagactcct tcatcgatta
    24241 tgcgatgagt gttattgtcg cacgtgcact tcctgatgtc agagatggtc tgaaacccgt
    24301 tcatcgccgt attttatacg gcatgaacga attaggtgta acaccggata aagcccataa
    24361 aaaatctgcc agaatcgtcg gagatgtcat gggtaaatac catcctcatg gagactatgc
    24421 gatctatgag tcaatggtac ggatggcaca gccattcagc taccgttata tgctagtgga
    24481 tggtcatgga aactttggtt ctgttgacgg tgacggtgcc gccgctatgc gttataccga
    24541 agcacggatg agcaaaatcg ctacagaaat gctgcgtgat atcaataaga acactgttga
    24601 tttccaaagc aactatgatg atacagaaaa agaaccagtt gtactacctg ctcgcttccc
    24661 gaatctttta gtgaacggaa caacggggat cgccgtaggt atggccacga atatcccacc
    24721 tcataacttg tctgaggtag tagcggctat cgatttgctg atggataatc ctgatgtgac
    24781 gaccaatgaa ttgatggaag tattacctgg accagatttt ccaacaggtg gtttggttat
    24841 gggaaaatca ggaatccgaa gagcgtatga aacaggtaag ggttctatta ccgtacgtgc
    24901 aaaagttgag attactgaga tgccaaatgg taaagaacgg atcatcgtga cagaattgcc
    24961 ttatatggtc aataaagcaa aattgatcga acggatttct gaactgcaca gagacaagcg
    25021 tatcgaaggg atcacagatt tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat
    25081 tgacatccgt cgtgacgcca gcgcatctgt tatcttgaat aacttatata aattgacatc
    25141 cttgcaaaat tcatttggat tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt
    25201 gagcttgaaa caaattcttg aaaactatat cgaacatcag cgcgaagtca tcacaagacg
    25261 gacagtcttt gaaaaagaaa aagcagaagc aagggcacac attttagaag gtttgcgtat
    25321 tgctttggat catatcgatg aaatcattgc gattatccgc gggtctaaat cagatgacga
    25381 agcaaaagcg acgatgatcg aacggttcga tttatccgat cgccagtctc aagctatttt
    25441 agatatgcgt ttacgccgat tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca
    25501 agaactctta aaactgattg aagatttaac tgacattctg gcaagaccag aacgtgttac
    25561 agaaatcatc aagaatgaat tagcagactt gaatcaacga tttggtgata aacgacgcac
    25621 agaattacta gtcggtgagg tcttaagtct ggaagatgaa gatttaatcg aagaagaaga
    25681 aatcgttatt acattgacaa acaatggtta catcaaacgt ttggcaaata atgaattcag
    25741 agctcaacga aggggtggac gtggtgtcca aggaatgggt atccacaatg acgacttcgt
    25801 caaaaatctg gtttcttgtt ctacccatga tacattgtta ttctttacga ataacggaaa
    25861 agtgtatcgg gcaaaaggat acgaaattcc agaatacggg cgcacagcca aaggaatacc
    25921 aattatcaat ttactaggta tcgattcgag tgaaagcatc caggcaatca ttgcggtgac
    25981 gggagaagca gaagaaggcc actatctctt ctttacgacg cgtcaaggta cagtaaaacg
    26041 tacttctgtc actgcttttg ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa
    26101 agaagacgat gagctcgtca atgtgctact gacagatggt caatctaata tcattattgg
    26161 aacacataac ggctactctg tcactttcaa agaagaagca gtccgcgata tgggacgtac
    26221 agcttccggg gttagaggaa tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt
    26281 aatgaaggaa gatcaagaag tattagtgat cacagaaaaa ggttacggaa aacggacaaa
    26341 agtatcggaa tatccagtga aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac
    26401 tgaaaaaaat ggtccactag ctggattgac cactgtttct ggagatgaag acatcatgct
    26461 gattacagat aaaggtgtca ttatccgttt caacgtatcg actgtttctc aaactggacg
    26521 ttcaacatta ggcgtacgtt tgatgaagat ggaagctgat acgaaagttg tcacgatggc
    26581 agcagtcgaa ccagaagctg cagaacaaac agttgaagaa caaggatcag aagaatagcc
    26641 atcaagacta attgtgtaag ctagcaagaa tgaccgatta aaaaatctgg ttattcctgc
    26701 tggctttttt gattagttga atttctctta aaatattcga aactgttcat tctgtattgc
    26761 ttttgaccaa aatagagtgt ataatcagag cttgtgagta gtctaattaa ttagataact
    26821 ctccttgctc ttaggaacct aagagccgaa agtccacagg gaggtgaata tcaatggaaa
    26881 acacgaaata tgaaatcatg tacattattc gtccaaacat tgatgaagaa gcaaaaactg
    26941 ctttaatcga acgtttcgat gcaatcttga aagataacgg agcagaagtt atcgaatcaa
    27001 aagattggga aaaacgccgt ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc
    27061 acatcgttaa agtatcttct ccatcatcag caaatgctgt caatgaattt gaccgtcttg
    27121 ctaaaatcaa cgacgatatc attcgtcaca tgattgttaa agaagaagct taattgttgt
    27181 ttcatgtgaa acaatagaaa acgatgagag gagatgacta ttttgattaa taatgttgta
    27241 ttagttggac gtctgacgaa agatccagat ttgcgttaca cagcaagtgg tacggcagtc
    27301 gcaacattta ctttggctgt gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct
    27361 gattttatca actgtgtgat ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt
    27421 aaaggaacac ttttaggtgt tactggacgt atccaaactc gttcttatga taatcagcaa
    27481 ggacaacgtg tatacgtgac agaagtagta gctgacaact tccaattgct ggaaagtcgt
    27541 tctgcatctg aaaatcgcca acaaagcggc ggattccaaa gttctggtca atcagctggc
    27601 ggatttggcg gaaacaataa ctctaatcaa acatctcaat catccaacgg tatgcctgat
    27661 ttcgaccgtg atacttctga cccattcggc tcaagttcaa cgatcgacat ttcagacgat
    27721 gatttaccat tctaaacgaa tttgatagga gggaaataga atggcacaac aaagaagagg
    27781 cggacgtaaa cgtcgtaaag tagactacat tgctgctaac catattgaat atatcgatta
    27841 taaagatatc gaattactaa aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg
    27901 tgtaactggt acaggcgcta aaaaccaacg taaaattact gttgcaatca aacgcgctcg
    27961 tatcatgggc ttgctacctt tcgttagcga agactaataa atcctaggaa ttgaatgact
    28021 gatccttttg atcagctatt caattccttt ttttctgtaa aaataacctc tattcgttta
    28081 gtcgctcgaa tgacgaaagt tatggtaaaa tcaaattagt catatatggg aggaaaatta
    28141 tggaaaataa aaacaatcat ttatccccga aacctctctt ccttatcctg tttatattaa
    28201 tcgaagtatt ggtagtctta attttaccat ttcgttttgt tagcgcttcg gtcattctat
    28261 tgatgaattt atggggtgtt cgctattggt tgaaaatgaa acgttggctt attttaaata
    28321 aacgtgaaaa aatccagcag gcttcgactg tagctgaaac caatctagct tatgtctcag
    28381 aagtcatgcc tgtaggagtg attgcttatc agccatcaag ccaaaaaatc gaatggctca
    28441 atccattagc attgaaaatc aaagatcaaa gtggattaac agaggaagaa ttgctagata
    28501 ctttttttac tgcaaaagaa aaaaagaaaa accaagtcta tctagcagat aaaacatttc
    28561 agtttgatat ggatacagta aagggtgttg tttattttaa tgatattacc caaaaccagt
    28621 tgttaaaact ccatcagacg aacagtcagc cggtgattgg tattttatct attgataact
    28681 atgcagatgc cattgataaa ttggatgaca aagagatttc ttatttgaac tctttcgtca
    28741 ctacactagt ttctgactgg atgaatgaat accatgtctt tttcaaaaga atcaacgcag
    28801 aacggttttt cttcgtagca cagatgatcg atctgaaaaa gatgatggat aaaaaatttg
    28861 atttgctgga tcgctttcga gcagaagctg aaaaacagag ctcgcctatc acccttagta
    28921 tggggattgc ttatggctca agaaccctcg atgaaatcgg tggagaagct cagaataatt
    28981 tagatgttgc tttagtccgt ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag
    29041 caaaacctgt ttattatggg gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt
    29101 ctcgagcgat gagtaccgct ttgcgtcgga tttttgatga aacaggcgat gtgtttatca
    29161 tggggcacaa gtttccggat atggatgctt taggctctgc ttttggtgtc agttgtctgg
    29221 cacgcttcaa ccagaaaaaa gcttacatcg ttatcaacga gaacgagatc attccagatg
    29281 tagaacggtg tcttgaagaa atccataagc atcctgaatt agaaatgcaa ctaatctctc
    29341 cagaacgtgc catagagctg aaaaaagaag aggacctcct cgtgatggtg gattaccata
    29401 aaccttctct atcgatctct cagggcctat atgagcaatt tgataagatt gtgatcattg
    29461 atcaccatcg tcgtggggaa gagtttccaa gtaaacctct gctgacttat atcgaatctt
    29521 cagcttcttc agcttcagag ctagtttctg agctgatcca ataccaaagc agcaaaacaa
    29581 atcgcttaga tcgttttgag gccacattat tgcttgcagg gatggttgta gatacaaata
    29641 gtttcaatgt ccgtacaact gcacgaactt ttgacgttgc cagctattta aggtcttgtg
    29701 gagcagacgc atccttagta caatatttat tgagctcgga tttagcgaca tacttggaga
    29761 tcagcaattt gattgctgga agcgactatg tgacttctga tattgttgtg gcggcaggga
    29821 cagacgaaaa agagtataat agtgttatag cagcaaaaac agcagataca ttgctttcga
    29881 tgatcaatat caatgcagct ttcgtcatta caaagcggac agacggcttg attgggatca
    29941 gtgccagaag taccggcacg atcaacgtcc aaatcatcat ggaatcctta ggtggcggag
    30001 gacacttcac aaacgcagct actcaactgg aaaatacgac agttgaggaa acaaaacaga
    30061 agctatttgc tgcaatcaaa gaaaatatga atcaaatata tgataaggag taatccaaat
    30121 gaaagtaatt tttttacaag atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt
    30181 tccagcaggt tatgcacaaa attatctaat caaaaataac ttggcaaaag aagcaacgaa
    30241 aagcagtatc agtcagttag caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat
    30301 cttggaagaa gctaaaaagt taaaagaatt cttggaaaaa gaagatactg tagtcgaatt
    30361 gaaggcgaaa gcaggggaag acagccgttt gtttggttcg atcccttcaa aacaaattgc
    30421 tgaagggtta aacaaacaat acaaaatcaa attagataaa cgaaaaatcg aattagaaaa
    30481 tccgatccgt tcactaggct acacaaacgt acctgttaag cttcatcatg aggtgacggc
    30541 tacgatcaaa gttcatgtcg tacaagaata acaaatcttg atagcgatgt tcagctggta
    30601 agtataacca gaaggaaaaa agacaaaccg aaatttatct gagaaaacag ctgcagcaat
    30661 gaaagcaaaa gctgcaaatc gatttcagat tgatttcggt ttcttttttg ggaacagcaa
    30721 aatagcataa agaaaacgag tataataaaa gagaactatt tttttattac actttcggta
    30781 cgaatgatga cttttctctc attttcttgt aaaatgaagc aattgaagac aaggagcgat
    30841 acgtatgaat gaagtgtggc aggatcgaat cccaccgcaa aatattgaag ctgaacaagc
    30901 ggttttaggc tctgtttttc ttgacgcaga aacgatcatt gatgccatgg agtacataga
    30961 gccaaaagac ttttatcgtc gtggccatca aattattttt gagacaatga tcgagttaaa
    31021 tgatcgtaat gaagcgattg acgtggttac tgtcaaagac cggctggaac aagccaattt
    31081 gttagaagat gccggtggtt taagttatct ctcagacttg gcattagcag ttccgacagc
    31141 agcaaatatc gtctactatg cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat
    31201 ccagacagca acaggaatcg ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat
    31261 tttagatgat gccgaacgca gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt
    31321 tttatctatt gctgaagtac tgaactccac gattgaaaat atcgatcgac tagctcaaaa
    31381 caatgaagaa atcacaggtc taccaactgg ctatcaaggt ctggataaaa tgacagcagg
    31441 tttccagccc gaagaactaa tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt
    31501 tgcattgaat attgctcaaa atgtaggaac aaaaacagat cgttctgttg cgatatttag
    31561 tttggaaatg ggggcagaat ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga
    31621 ggccagccat ttacgtacag gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc
    31681 gatggggagt ctttctcgag caaatatttt tatcgatgat acaccaggga tcaaaatctc
    31741 tgaaatccgt gcgaaatgtc ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt
    31801 gatcgattat ttgcagttga ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc
    31861 agatatctct cgccaactga aaaaactggc gaaagagctg aaagtacctg tcattgctct
    31921 ttcacagtta tctcgtggag tagaacagcg acaagacaaa cgaccagtgc taagtgatat
    31981 tcgtgaatcc ggatcaattg aacaagatgc ggacatcgta gccttcttgt atcgagatga
    32041 ttattatgaa agagaaggcg gagatgacga tgatggaaac gagccaaaaa atgacaacgt
    32101 catcgaagtc atcatcgaga agaaccgaag cggtgcacga ggaacagttg aactactgtt
    32161 tatcaaagaa tacaataagt tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg
    32221 ttgtgagcct tgagcaatcc cgagagctct cgtaagaaga tgcaaggagt aaccgcagga
    32281 acgactagag tacttaattt aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac
    32341 agtccctttt tccgaataaa cggtgttcaa tcagctgacc cttggtatta gctcagcaaa
    32401 cgtcaaaata tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg
    32461 atcttacgac ctcttttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac
    32521 aatatcatca aacatgagaa gcagtttttt gaaattgctc cttgtgactc agagttaaac
    32581 ggcaggctca caacctcttt cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc
    32641 ccaatcaaac gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca
    32701 ggacagcttt ttcacaacct ctgattcacg gtgttccaaa agtagcttct tcgaaaaata
    32761 agtcgaaatt tcacaaaaat ttgagaagca attttcgtac attccttctt atttcttgaa
    32821 gctgaccact tctgttacaa cctctttttt aataaccatt ctaaaaagat aaagttcgtt
    32881 ttttaagcgt ttttataaaa taaaaataaa aatcattcga ttttatttga aatatgtatc
    32941 tattattgat acaatggttc aggattaaat aatatagaac gaggtgttca catgtcatct
    33001 gtagtagtag taggtacgca atggggcgat gaagggaaag gaaaaatcac agactttctc
    33061 agtgaaaatg ctgaagtgat tgcacggtac caaggtggcg acaatgcagg gcacacaatt
    33121 aagtttaacg gtgtgactta taaactgcat ttgattcctt ctgggatttt ttataaagac
    33181 aagatcagtg tgatcggaaa cggcgttgta gtcaatccta aatcattggt taaagaatta
    33241 gcttatttac atgaaaaagg tgtagaaaca agtaatttgc gtatttctga ccgcgcgcac
    33301 gtaatcttgc cttatcatat caaattggac caattacaag aagatgctaa gggcgaaaat
    33361 aagattggta caacgattaa aggaatcgga cctgcctata tggataaagc agctcgtgta
    33421 ggtatccgta ttgcagactt attagataaa gaaatttttg aagaacgtct tcgtttgaat
    33481 ctagatgaca aaaaccgtca atttgtaaaa atgtttgatt cagaaccact tgattttgac
    33541 gatatttttg aagaatatta tgaatacgga caacaaatca aacaatacgt cacagatact
    33601 tccgttatct tgaatgatgc tttggatgca ggcaaacgcg tgttgtttga aggcgcacaa
    33661 ggtgtaatgc tggatatcga ccagggaact tatccttttg ttacttcttc taacccagta
    33721 gccggtgggg taacaatcgg aagcggtgta gggccttcaa aaatcaataa agttgtcggc
    33781 gtctgcaaag cctatacgtc acgtgtcggt gatggaccat ttccaactga attatttgat
    33841 gaaacaggag aacaaatccg cgaagtagga cgagaatacg gtacgacaac tggtcgtccg
    33901 cgtcgtgtag gctggtttga ttctgtagtt atgcgccatt ctaaacgagt ttcaggtatt
    33961 accaacctat ctttaaactc gatcgatgta ttaagtggat tggaaacagt aaaaatttgt
    34021 acggcttatg aattagatgg cgaactaatc taccactatc cagcaagctt gaaagagttg
    34081 aaccgctgca aaccaatcta tgaagaattg ccaggctggt cagaagacat tacaggatgc
    34141 aaaacacttg cagaactgcc agaaaacgca cgtaactatg tacgtcgtat ctcagagctt
    34201 gttggtgtac gtatctcaac attttctgtc ggacctgata gaacacaaac aaacatctta
    34261 gaaagcgtct gggcgcaaat ctaaggataa aaacgacgac tgaatcagga cgttcttacc
    34321 agagcgagtc gaagtgttta tctatacgat aataataact tgaagaggta gtgaaagaga
    34381 cgtttagcct tattgccgat aggctgtctc ttgaacactg tttattca
//
LOCUS       DASJST010000010        34393 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJST010000010 DASJST010000000
VERSION     DASJST010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.010"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(44..1936)
                     /locus_tag="U4B05_000373"
     CDS             complement(44..1936)
                     /locus_tag="U4B05_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049538.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1933..3660)
                     /locus_tag="U4B05_000374"
     CDS             complement(1933..3660)
                     /locus_tag="U4B05_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049539.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3676..4326)
                     /locus_tag="U4B05_000375"
     CDS             complement(3676..4326)
                     /locus_tag="U4B05_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2049540.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4530..4826
                     /locus_tag="U4B05_000376"
     CDS             4530..4826
                     /locus_tag="U4B05_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2049541.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5018..5638
                     /locus_tag="U4B05_000377"
     CDS             5018..5638
                     /locus_tag="U4B05_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN2049542.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5730..6896)
                     /locus_tag="U4B05_000378"
     CDS             complement(5730..6896)
                     /locus_tag="U4B05_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2049543.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6908..7261)
                     /locus_tag="U4B05_000379"
     CDS             complement(6908..7261)
                     /locus_tag="U4B05_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2049544.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7389..8411
                     /locus_tag="U4B05_000380"
     CDS             7389..8411
                     /locus_tag="U4B05_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN2049545.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8430..8591
                     /locus_tag="U4B05_000381"
     CDS             8430..8591
                     /locus_tag="U4B05_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049546.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8850..9284)
                     /locus_tag="U4B05_000382"
     CDS             complement(8850..9284)
                     /locus_tag="U4B05_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049547.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9394..11253)
                     /locus_tag="U4B05_000383"
     CDS             complement(9394..11253)
                     /locus_tag="U4B05_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2049548.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11371..12531)
                     /locus_tag="U4B05_000384"
     CDS             complement(11371..12531)
                     /locus_tag="U4B05_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN2049549.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="U4B05_000385"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="U4B05_000385"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN2049550.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="U4B05_000386"
     CDS             complement(14278..14976)
                     /locus_tag="U4B05_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2049551.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="U4B05_000387"
     CDS             complement(14933..16594)
                     /locus_tag="U4B05_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN2049552.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="U4B05_000388"
     CDS             16812..18212
                     /locus_tag="U4B05_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN2049553.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18464..19543)
                     /locus_tag="U4B05_000389"
     CDS             complement(18464..19543)
                     /locus_tag="U4B05_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2049554.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19629..20588)
                     /locus_tag="U4B05_000390"
     CDS             complement(19629..20588)
                     /locus_tag="U4B05_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2049555.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20600..22111)
                     /locus_tag="U4B05_000391"
     CDS             complement(20600..22111)
                     /locus_tag="U4B05_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2049556.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLKSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22133..22697)
                     /locus_tag="U4B05_000392"
                     /pseudo
     CDS             complement(22133..22697)
                     /locus_tag="U4B05_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(22703..23566)
                     /locus_tag="U4B05_000393"
     CDS             complement(22703..23566)
                     /locus_tag="U4B05_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2049557.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23580..24476)
                     /locus_tag="U4B05_000394"
     CDS             complement(23580..24476)
                     /locus_tag="U4B05_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2049558.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24651..25940)
                     /locus_tag="U4B05_000395"
     CDS             complement(24651..25940)
                     /locus_tag="U4B05_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2049559.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25912..27429)
                     /locus_tag="U4B05_000396"
     CDS             complement(25912..27429)
                     /locus_tag="U4B05_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2049560.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27627..28517
                     /locus_tag="U4B05_000397"
     CDS             27627..28517
                     /locus_tag="U4B05_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN2049561.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="U4B05_000398"
     CDS             complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="U4B05_000398"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN2049562.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29426..30898)
                     /locus_tag="U4B05_000399"
     CDS             complement(29426..30898)
                     /locus_tag="U4B05_000399"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN2049563.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31535..31897)
                     /locus_tag="U4B05_000400"
     CDS             complement(31535..31897)
                     /locus_tag="U4B05_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2049564.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32120..32872
                     /locus_tag="U4B05_000401"
     CDS             32120..32872
                     /locus_tag="U4B05_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2049565.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33012..33935)
                     /locus_tag="U4B05_000402"
     CDS             complement(33012..33935)
                     /locus_tag="U4B05_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2049566.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFLDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33950..34222)
                     /locus_tag="U4B05_000403"
     CDS             complement(33950..34222)
                     /locus_tag="U4B05_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049567.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10149 a         7164 c         5911 g        11169 t
ORIGIN      
        1 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
       61 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      121 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      181 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      241 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      301 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      361 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      421 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      481 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      541 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      601 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      661 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      721 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
      781 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
      841 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
      901 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
      961 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1021 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1081 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1141 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1201 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1261 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1321 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1381 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1441 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1501 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1561 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1621 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1681 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1741 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     1801 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     1861 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     1921 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     1981 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2041 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2101 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2161 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2221 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2281 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2341 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2401 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2461 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2521 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2581 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2641 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2701 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     2761 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     2821 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     2881 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     2941 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3001 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3061 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3121 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3181 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3241 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3301 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3361 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3421 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3481 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3541 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3601 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3661 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3721 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     3781 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     3841 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     3901 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     3961 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4021 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4081 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4141 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4201 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4261 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4321 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
     4381 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4441 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4501 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4561 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4621 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4681 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4741 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     4801 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     4861 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     4921 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
     4981 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5041 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5101 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5161 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5221 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5281 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5341 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5401 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5461 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5521 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5581 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5641 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
     5701 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5761 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     5821 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     5881 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     5941 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6001 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6061 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6121 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6181 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6241 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6301 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6361 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6421 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6481 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6541 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6601 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6661 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6721 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6781 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     6841 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     6901 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     6961 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7021 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7081 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7141 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7201 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7261 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7321 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7381 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7441 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7501 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7561 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7621 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7681 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7741 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7801 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     7861 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     7921 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     7981 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8041 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8101 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8161 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8221 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8281 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8341 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8401 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8461 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8521 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8581 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8641 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8701 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8761 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     8821 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     8881 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     8941 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9001 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9061 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9121 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9181 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9241 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9301 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9361 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9421 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9481 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9541 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9601 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9661 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9721 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9781 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
     9841 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
     9901 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
     9961 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10021 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10081 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10141 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10201 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10261 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10321 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10381 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10441 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10501 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10561 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10621 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10681 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10741 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10801 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    10861 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    10921 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    10981 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11041 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11101 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11161 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11221 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11281 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11341 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11401 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11461 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11521 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11581 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11641 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11701 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttgcctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt
    18301 ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga
    18361 gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga
    18421 cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt
    18481 tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt
    18541 tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg
    18601 tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga
    18661 tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag
    18721 ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta
    18781 ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca
    18841 aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt
    18901 gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt
    18961 ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt
    19021 cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg
    19081 ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat
    19141 aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta
    19201 aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa
    19261 aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg
    19321 ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat
    19381 agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata
    19441 tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga
    19501 tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt
    19561 atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt
    19621 tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc
    19681 cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg
    19741 ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta
    19801 aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc
    19861 gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg
    19921 cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg
    19981 cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca
    20041 ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga
    20101 taaagattag aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt
    20161 ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt
    20221 cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg
    20281 cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac
    20341 cattttccat tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct
    20401 tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc
    20461 cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct
    20521 gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc
    20581 ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa gagtattttt
    20641 ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta
    20701 ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt
    20761 gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg
    20821 tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat
    20881 aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata
    20941 cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg
    21001 cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac
    21061 taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg
    21121 tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc
    21181 ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac
    21241 ttttcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga
    21301 atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag
    21361 tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct
    21421 cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag
    21481 agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt
    21541 catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta
    21601 gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc
    21661 ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt
    21721 catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga
    21781 tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat
    21841 caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg
    21901 taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga
    21961 catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat
    22021 agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta
    22081 ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt
    22141 catttcctat agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa
    22201 taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa
    22261 tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg
    22321 tgatatcttt gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa
    22381 gtaaaactac atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa
    22441 caagaaaata aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct
    22501 ttattccttg aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca
    22561 ttacataaaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
    22621 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta
    22681 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
    22741 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
    22801 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
    22861 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
    22921 tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
    22981 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
    23041 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
    23101 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
    23161 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
    23221 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
    23281 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
    23341 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
    23401 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
    23461 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
    23521 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
    23581 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
    23641 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
    23701 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
    23761 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
    23821 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
    23881 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
    23941 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
    24001 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
    24061 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
    24121 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
    24181 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
    24241 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
    24301 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
    24361 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
    24421 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
    24481 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
    24541 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
    24601 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
    24661 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
    24721 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
    24781 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
    24841 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
    24901 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
    24961 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
    25021 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
    25081 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
    25141 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
    25201 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
    25261 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
    25321 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
    25381 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
    25441 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
    25501 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
    25561 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
    25621 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
    25681 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
    25741 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
    25801 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
    25861 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
    25921 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
    25981 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
    26041 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
    26101 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
    26161 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
    26221 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
    26281 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
    26341 ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc
    26401 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
    26461 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
    26521 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
    26581 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
    26641 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
    26701 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
    26761 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
    26821 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
    26881 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
    26941 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
    27001 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
    27061 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
    27121 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
    27181 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
    27241 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
    27301 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
    27361 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
    27421 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
    27481 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt
    27541 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
    27601 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
    27661 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
    27721 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
    27781 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
    27841 tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt
    27901 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
    27961 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
    28021 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
    28081 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
    28141 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
    28201 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
    28261 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
    28321 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
    28381 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
    28441 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
    28501 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
    28561 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
    28621 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
    28681 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
    28741 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
    28801 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
    28861 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
    28921 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
    28981 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
    29041 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
    29101 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
    29161 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
    29221 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
    29281 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
    29341 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
    29401 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
    29461 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
    29521 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
    29581 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
    29641 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
    29701 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
    29761 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
    29821 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
    29881 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
    29941 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
    30001 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
    30061 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
    30121 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
    30181 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
    30241 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
    30301 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
    30361 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
    30421 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
    30481 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
    30541 gcccttccac ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac
    30601 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
    30661 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
    30721 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
    30781 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
    30841 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
    30901 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
    30961 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
    31021 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
    31081 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
    31141 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
    31201 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
    31261 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
    31321 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
    31381 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
    31441 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
    31501 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
    31561 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
    31621 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
    31681 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
    31741 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
    31801 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
    31861 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
    31921 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
    31981 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
    32041 cgtgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
    32101 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
    32161 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
    32221 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
    32281 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
    32341 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
    32401 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
    32461 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    32521 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    32581 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    32641 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    32701 ccaagctcat aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa
    32761 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    32821 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    32881 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    32941 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    33001 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    33061 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    33121 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    33181 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    33241 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    33301 aaaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    33361 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    33421 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    33481 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    33541 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    33601 gcaaattctt caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc
    33661 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    33721 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    33781 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    33841 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    33901 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    33961 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    34021 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    34081 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    34141 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    34201 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    34261 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    34321 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
    34381 attattagtg aat
//
LOCUS       DASJST010000011        34304 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJST010000011 DASJST010000000
VERSION     DASJST010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.011"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            117..797
                     /locus_tag="U4B05_000404"
     CDS             117..797
                     /locus_tag="U4B05_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2049568.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            887..1921
                     /locus_tag="U4B05_000405"
     CDS             887..1921
                     /locus_tag="U4B05_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2049569.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1935..2270
                     /locus_tag="U4B05_000406"
     CDS             1935..2270
                     /locus_tag="U4B05_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049570.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2254..4734
                     /locus_tag="U4B05_000407"
     CDS             2254..4734
                     /locus_tag="U4B05_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049571.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            4790..6349
                     /locus_tag="U4B05_000408"
     CDS             4790..6349
                     /locus_tag="U4B05_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2049572.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6346..7710
                     /locus_tag="U4B05_000409"
     CDS             6346..7710
                     /locus_tag="U4B05_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2049573.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7881..8951
                     /locus_tag="U4B05_000410"
     CDS             7881..8951
                     /locus_tag="U4B05_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049574.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8966..9634
                     /locus_tag="U4B05_000411"
     CDS             8966..9634
                     /locus_tag="U4B05_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049575.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9654..10232
                     /locus_tag="U4B05_000412"
     CDS             9654..10232
                     /locus_tag="U4B05_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2049576.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10633..11544
                     /locus_tag="U4B05_000413"
     CDS             10633..11544
                     /locus_tag="U4B05_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2049577.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11632..12549)
                     /locus_tag="U4B05_000414"
     CDS             complement(11632..12549)
                     /locus_tag="U4B05_000414"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN2049578.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12751..13188
                     /gene="zapA"
                     /locus_tag="U4B05_000415"
     CDS             12751..13188
                     /gene="zapA"
                     /locus_tag="U4B05_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN2049579.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13247..13795
                     /locus_tag="U4B05_000416"
     CDS             13247..13795
                     /locus_tag="U4B05_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN2049580.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            13910..16270
                     /locus_tag="U4B05_000417"
     CDS             13910..16270
                     /locus_tag="U4B05_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN2049581.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16357..16671
                     /gene="trxA"
                     /locus_tag="U4B05_000418"
     CDS             16357..16671
                     /gene="trxA"
                     /locus_tag="U4B05_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN2049582.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            16853..18631
                     /gene="uvrC"
                     /locus_tag="U4B05_000419"
     CDS             16853..18631
                     /gene="uvrC"
                     /locus_tag="U4B05_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN2049583.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18776..19675
                     /locus_tag="U4B05_000420"
     CDS             18776..19675
                     /locus_tag="U4B05_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049584.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19668..20930
                     /locus_tag="U4B05_000421"
     CDS             19668..20930
                     /locus_tag="U4B05_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049585.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21091..22989
                     /locus_tag="U4B05_000422"
     CDS             21091..22989
                     /locus_tag="U4B05_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN2049586.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23319..24038)
                     /locus_tag="U4B05_000423"
     CDS             complement(23319..24038)
                     /locus_tag="U4B05_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN2049587.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24375..24707
                     /locus_tag="U4B05_000424"
     CDS             24375..24707
                     /locus_tag="U4B05_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049588.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24694..26697
                     /locus_tag="U4B05_000425"
     CDS             24694..26697
                     /locus_tag="U4B05_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN2049589.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26716..27186
                     /locus_tag="U4B05_000426"
     CDS             26716..27186
                     /locus_tag="U4B05_000426"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN2049590.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27207..27791
                     /locus_tag="U4B05_000427"
     CDS             27207..27791
                     /locus_tag="U4B05_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN2049591.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27806..28792
                     /locus_tag="U4B05_000428"
     CDS             27806..28792
                     /locus_tag="U4B05_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN2049592.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            28782..29093
                     /locus_tag="U4B05_000429"
     CDS             28782..29093
                     /locus_tag="U4B05_000429"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN2049593.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29115..30896
                     /locus_tag="U4B05_000430"
     CDS             29115..30896
                     /locus_tag="U4B05_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN2049594.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            30889..32265
                     /locus_tag="U4B05_000431"
     CDS             30889..32265
                     /locus_tag="U4B05_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN2049595.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32272..32907
                     /locus_tag="U4B05_000432"
     CDS             32272..32907
                     /locus_tag="U4B05_000432"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN2049596.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            32937..34292
                     /locus_tag="U4B05_000433"
     CDS             32937..34292
                     /locus_tag="U4B05_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN2049597.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        11340 a         5913 c         7363 g         9688 t
ORIGIN      
        1 tttttctctt ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat
       61 tttttgcata ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga
      121 aaaagaacat gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac
      181 cagcttttgc tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa
      241 ataccgtgga gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg
      301 aaaatccagc aactggccag acaggtcctt taacactgga tgtagttccc gaattgccat
      361 ttggtacaca cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg
      421 atacacctta tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag
      481 ggtggaacgt gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg
      541 ctgaattaga ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac
      601 caactagtca aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag
      661 cagcaaaaga tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac
      721 tgaaagtgcc gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg
      781 ctggcccagt agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg
      841 tgaaggattt tttaccgcag tttttgttga aaggatgtgg tggacaatga atcaaagtaa
      901 acgactgttt gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag
      961 ccaagcaaag gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat
     1021 agataaagca atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga
     1081 agtaattatc agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc
     1141 ggtaacgagt gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac
     1201 attgccgttt gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc
     1261 acatgcagaa acaactgtac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg
     1321 tgtattagcg ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga
     1381 aggagcacaa atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca
     1441 agagacgata gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa
     1501 gccaactgtt cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt
     1561 tacgagcaag atcttttatg aaaatcagct ttatattgaa aatacgtcta atgcattttt
     1621 agttgcacca aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc
     1681 cggcgattac cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac
     1741 acaaaacttt acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc
     1801 agtacaagaa caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt
     1861 atggttgatt ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg
     1921 atgaggtgat ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt
     1981 tcttcagctg gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt
     2041 atgatctaag tccaattccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga
     2101 cacagctacc gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaac
     2161 aaaagatccg ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt
     2221 tgttatggag aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt
     2281 tttcgctcct tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg
     2341 gcagaagctg ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat
     2401 atcgtacctc caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact
     2461 ggggctaacc aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct
     2521 tggaaaggtg gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt
     2581 gatttggtga atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa
     2641 catgtatcaa gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt
     2701 gccggtgccc ccatcaaggt tactagcgga agtttattat cagcaggaag ccataccttg
     2761 cgttttggga tggggtcgtg ggatacaaat actctattga cagatattat tgctattatt
     2821 acgcgaggcg aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc
     2881 gcgaagatga cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag
     2941 ggtaaattac aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg
     3001 aataatatta tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt
     3061 tatcctaaat taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca
     3121 gagataaata cgtggaatag ttacatctca ccaagtgatt caacaggaaa atataataca
     3181 tttgctgata ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat
     3241 agtcggacat tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc
     3301 tttgatcgcg tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact
     3361 catactccag caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa
     3421 gatgcgcaag gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta
     3481 gacggaacgg ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct
     3541 tctgcatcgt accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc
     3601 cattacaggc tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt
     3661 ttccaaacga tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca
     3721 ttggcttcag ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg
     3781 tatccgtctt ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa
     3841 ataacagtgg ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga
     3901 caacctgaga caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg
     3961 acattcacgt taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt
     4021 cctgaaaata ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat
     4081 accggcattc aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc
     4141 attcatctta cttacaacta tcaattaaat actgtagata cttctttaca atccgtcagc
     4201 atactgacaa aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt
     4261 cctaacccag ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca
     4321 ttagttaatg taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca
     4381 agttattatg aatcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc
     4441 acgtctcctt ggcaactgac aggtacattg acttctttat ttaagaatga tcaaggtcaa
     4501 gaattaagcg gaacaaaatt atacttcaat cattctggat caaagcaatt gattcagcaa
     4561 ggtcaaaaca cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac
     4621 tttcccgata ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc
     4681 gcaacgtatc aaggcatggt cacatggaaa ctgacagctg ggccaaccag ctaagtgatt
     4741 agaatttgta tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa
     4801 acttctgctg ttaaaatcag aaatgcttga aggagtggac ttggaacttt tcagtttgct
     4861 tgaaaaaaat gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga
     4921 ggtttccgtt aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga
     4981 tctgttttat ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga
     5041 agggaatatt ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg
     5101 ttacttgcgc tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt
     5161 tgatcggcag aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt
     5221 gaaggaatta aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac
     5281 cggtccagaa agtcaaattc gttattttta tttccaatta ttttggtttg gacgtcccta
     5341 tcttgaaaac aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca
     5401 aaaagagttt agttttgcta ttacagaaga aggacgattg aaatttgaaa tatggctttc
     5461 cattatgaag aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc
     5521 tgttggattg tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta
     5581 cttctttcaa tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat
     5641 gatcagtaac tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga
     5701 aaaatacaaa gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga
     5761 atatttctct ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat
     5821 gcaaaatcac gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg
     5881 attaaaacag cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt
     5941 ggtcagacaa acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt
     6001 tgaattattt gctcgctatg catctatttt acggacgctg atcaagcagt tagaattacg
     6061 tctgcaaatt gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat
     6121 catccaggaa caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa
     6181 gcagtatgat atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa
     6241 agtttttgta ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt
     6301 aaaagaatgt tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc
     6361 attggtgctt cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa
     6421 gcagaagttt tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga
     6481 atcaaccttt atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg
     6541 actcttcaag aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca
     6601 gacaaacatc agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat
     6661 aaattaatcg tagcgacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa
     6721 aatgtctaca cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa
     6781 gcaaaagaag tagtgatctt cggtgctggt tacattggat tggaattagc agatgctttg
     6841 agaaataaag gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat
     6901 ttcgataagg atatgatcaa cagttttcaa aatcagctgc aaactaagca aataaatttt
     6961 tatccaaatg aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa
     7021 cttctgtctc aaacaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat
     7081 acgacattgt taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa
     7141 tatctgcaga caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt
     7201 gacaaaaata aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga
     7261 gcagtagcgc taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca
     7321 gcagcagtca ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca
     7381 ttcttggaac aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgac
     7441 gaagaaaaca aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt
     7501 ggcggacagc tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc
     7561 gtgaaacaca aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa
     7621 tacacctctc gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca
     7681 aatagcaata gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga
     7741 tataataaat attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt
     7801 ttttgagcat aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt
     7861 atttcaggag gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac
     7921 gttatgcgtt agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg
     7981 ggttagctta tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt
     8041 cgattgtgct ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga
     8101 tagcagaaga agtcgaaggt gggaaagtgg cgtatcttgc ccaaacacct ggagtcgtga
     8161 ctagtaaaga cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa
     8221 atcaattcat catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag
     8281 taggcgatat tagtttgaaa gaagaatatg gtctagccat aggagataca gttaagttat
     8341 ctggtagtga caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct
     8401 caccagttct ctatacaacc atcaatgctt accaagaaat ccgcttcgaa aaagaagata
     8461 caagtgaaaa tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg
     8521 aagatttaga acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc
     8581 aagtattgac ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg
     8641 ggattttcat ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac
     8701 aagggatcac aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat
     8761 ttgttggtat tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg
     8821 cggttccatt ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgttagtag
     8881 tagctgtgtt aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg
     8941 caattggata agaaggagga agaacatgaa agcaatagaa tttaaagaag tagaaaaaag
     9001 tttccaagat ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa
     9061 aggggaattt gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt
     9121 ggcaggcgga ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag
     9181 taatcaaaaa gaaagcgaac gcgttaaact acgttttaaa gaaattggct ttattttgca
     9241 agcttccaat ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat
     9301 taaacatgaa catcgtcaaa aagaagcaaa cgaattattt gagcagcttg gcgtggataa
     9361 gttgatcaac aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc
     9421 tcgtgcattg tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga
     9481 ttcagaaaag gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa
     9541 agcaacgatc atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat
     9601 gatggaagat ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc
     9661 atcgatacaa caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt
     9721 ccaagggata tctcggcact tcaacaagag aaatcgcaca aaaagttggg atcacccagc
     9781 ccaatctcta tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc
     9841 acttaaaaga tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc
     9901 aagtaacctt gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc
     9961 tgatgcttca cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc
    10021 tatggaagaa aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc
    10081 taagaaatgg gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt
    10141 acatcactcg gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt
    10201 tatatttgaa tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaatagttag
    10261 gtgaagaagc aagatatgac gaagatcgtt atatcttgct tctttatata ggtagttttt
    10321 cataaagaca aatttgttat tctctcatga ttatcagggt atatttttat ttattatgtc
    10381 ggtgttccag gaagtgtttt aatttaataa cgaagaaatt aattgtttgt agaatgaagg
    10441 aaactattta ttgggtagat gaatcaccac gaatagtcaa aaggagattt caggatatgc
    10501 caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc tacggttata tcttttacag
    10561 cccctttttt gcttacttaa gctgatttta agttttggcc cttagtctgg atctacgagg
    10621 tgagagtcaa gaatgaaaaa aataattgga gcttttgtat ttgcaatcat tatcattatt
    10681 atctgttttc aaatagatgt ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt
    10741 acgaacagta cggttacaaa gacaaaggaa tcatttgatg atacagaaca agagacatta
    10801 atcaaaatcg gaaaaaatga ccgtacacta tatacagatg actcgctaac taccccactt
    10861 gcaactataa acggcggaga gctggcggag tttctttctg aaacaaaaaa tgcttatcag
    10921 atcaagacaa atgatggcta tactggatat ttagccctcg aagatggaac caaagtcaca
    10981 aagaatatac aaacaaaacc ggaagagcta tctgatgcag tgattgtatt agatcctggg
    11041 catggcggaa atgatactgg ggcattaagc aatgatgaac aaacagaaga aaaagatata
    11101 acattaagta cagccataaa agtaaaaaag gcgctagagg atgctggagc aacggtttat
    11161 ttgacccata ctacagatga attagtacag cttggagata tatgcgacta cagtgaagaa
    11221 aaaaaggcag atgtctttat cagtttacac gcagactcta cggaatatgc gaatgaagca
    11281 actggaatca cgacatatta ttactatgga caagaggaaa cattagcaca gacaattgct
    11341 gactccttca ctgatctgcc tctggattca agaggaatct ccactgggaa ctatcaagtt
    11401 cttcgagaaa atcttcaacc ttctatttta atcgagatgg gttatatgaa taatgatagt
    11461 gatttaaagg aattagtaac agacagttat cagcagcaga ttgctgcaag tttgacacaa
    11521 gcattgacca cttattttca gtaataaata agcccagcca aacaaaaata gctcctcata
    11581 tttttcgttt ggccgggctt attatcgcag atgcaagctt ttgaacaccg tttatttttt
    11641 tagtagcttc aaagcttttt ctgtatttgc aaagtgaagt ttggcatatt tttcaagcat
    11701 aggcatgccg cctttctcca atagttttgc cgctacttgg tcagatttcg ttccagctcc
    11761 tgatggaagg gtgattccag cttcagctga tgctttgtgt aactcttcca aaaagctggc
    11821 acgactgatg atagaagcag cagcaacagc taaatgatat tgttctcctt tagtaacgaa
    11881 gtacagtttt tcttgtaatt gatttttttc taaacgcgca tattttttaa aatttgcttc
    11941 cggcgtaaac tgatcgatca aaatcgcttc tggtttttct ggctcgattt tttttagcag
    12001 caaatggatc gcttgattgt gcaaagcgac tttcatccga acggcattgt attttggttg
    12061 gatctcattg tatttttctg gagataagac aagcagttta taggggatgg ttttttttaa
    12121 gatgtcagca agctggatga tttggctatc ttttaactct tttgaatctc taacaccaag
    12181 tttacgtaat tctgcaagct gatcacgttt cacataagca gcacatactg ttagcggacc
    12241 gaaatagctg ccatttccga cctcatcact acctaaaacg gataattgac tgatattttt
    12301 tggcaaagaa gaggctgttt ttgctggctt ggcttgttta tcctgtgcct ccccccattt
    12361 atcagcttct attgctgcat tcgtaccttg aaacatcact tttccggatg tataagcggt
    12421 aatggtcgtc ccgtcttttt ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa
    12481 ataagagcga tagtatgctt tcatttgact aagttggtcg tttgttgctt tgataatgac
    12541 gttgctcata aaattctcct cgtttttata cataaaacta taccgcaact cacgactttt
    12601 cgttttctaa gagattgcga gacatagcat ttttttaaag tttatcatat aatagttaag
    12661 tatgtgaaaa atatttgtgg agaacggtga aagaaccaac ttcatggtac actaaataaa
    12721 actttcatca aataggagga aatgattcgt atggcgcacg aaaaaacacg ctacaaagcg
    12781 gtgattgcca accaaacata tactattatc ggacgggaaa ccaagcatca tatggatatc
    12841 gtaacgaagc tgatcaatga acagttagca gaattgaaac aattatcacc acaaatggat
    12901 aatgaacaag cagcaatctt gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa
    12961 gaaagaatct tggagctgga agaagaaaca gcagaattaa agaaaaaaat gatcaaattt
    13021 acagagcttg aaaatcgtgt gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta
    13081 ttgaaagaaa atggtcaagc agatcatacc attaaaaatc atgtggaagc acaacagatt
    13141 ttaaacgaga aacgaaaagg acaaattaaa caaaaatctt ctaattaaga tccttttatc
    13201 aaaataagaa aaatttaaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct
    13261 gattttattt ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca
    13321 aatcatattc gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc
    13381 attagcaagt catttggaat tatacattcc atacccagct gtcacgccaa catcaaagtt
    13441 agcatttttt gatcaggtat ttgcctttca cctagatgaa gctttttatg caggaactgc
    13501 ttttctgatt attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca
    13561 tggattgacg tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc
    13621 actaatcatg gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt
    13681 agatgtggtc caaaatcaat tcggcgagca tagtttcgct cgctttatcg ttgagagaac
    13741 gccatttctt accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa
    13801 atagtgctgg aaaatatttc gttttttgta cagagtagat tatttaagaa ggctgcgtag
    13861 cgtatgtccg gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg
    13921 aatccttgaa acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac
    13981 tgcacaagga aaagaagaac tggcagaatt ggtaccagtg agcgaggaac aaacgatcac
    14041 aaactggctt caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc
    14101 tatacctaaa cttgaaaata tccgtcccca tatgaaacgg attgagatcg gagcagactt
    14161 gaatggagtg gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg
    14221 tttcattgat gatctttatg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga
    14281 tcagttagtc acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg
    14341 acgcgtgact gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag
    14401 tgaacaagct gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt
    14461 aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata
    14521 ccgcggtgta tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat
    14581 tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg
    14641 aaatgagatt caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga
    14701 aatcatccac aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt
    14761 tggtaaagaa gtaaaagcta tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa
    14821 acaagcaaga catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg
    14881 aaaagactat caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt
    14941 gaagacttta ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga
    15001 agaaagtcaa atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat
    15061 tgagcaaagt ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa
    15121 agtcaatgaa aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga
    15181 aggtgctgct ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat
    15241 ggcaacaacg cattatcctg aattgaaagt ttatggatat aatcgggcaa atacgattaa
    15301 tgccagcatg gagtttgatg tggatacatt aagtccaacg tatcgtttgc tgattggcgt
    15361 tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat
    15421 tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga
    15481 tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc
    15541 tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg
    15601 tgaaaaagaa atggaaaaag caaagaaaaa agcaaatgaa gtcgtgtcag aggcggaaga
    15661 aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa
    15721 tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga
    15781 aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg
    15841 agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa
    15901 tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc
    15961 tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag
    16021 tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga
    16081 ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca
    16141 cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag
    16201 cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa
    16261 atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta
    16321 tactagtcat agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag
    16381 acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt
    16441 ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa
    16501 gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt
    16561 atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt
    16621 ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca
    16681 gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga
    16741 gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt
    16801 ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga
    16861 acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga
    16921 taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc
    16981 atattttact ggtagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt
    17041 tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa
    17101 gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa
    17161 gataacgaat gaaaaatatc cccgtttggt gattactcgt aaggtactaa aagacaaagc
    17221 actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga
    17281 tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta
    17341 tcatttggga caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa
    17401 aatcgttgat gaagtgaaaa actttttaaa cggaggacac gaaaaaatcg agtcagagat
    17461 ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg
    17521 agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt
    17581 actggatcga gatgtatttg gttatgcgat tgataaagga tggatgtgtg tccaagtgtt
    17641 tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga
    17701 agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat
    17761 tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc
    17821 tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgagtaa
    17881 gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac
    17941 gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga
    18001 gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat
    18061 tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc
    18121 ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcgcgag tgattcgcga
    18181 agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc
    18241 aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa
    18301 cgataaacat aaaacaaatg agttgctatt cggtacagat ttggctgtag taccgcttga
    18361 gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc
    18421 aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat
    18481 tgaagggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa
    18541 tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca
    18601 aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag
    18661 tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat
    18721 cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt
    18781 agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt
    18841 ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac
    18901 gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg
    18961 ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg aacggggact
    19021 ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc
    19081 aagaaaagag attgaaccaa aaatcgactt ttggatggaa aaatttcagg taaaagggaa
    19141 aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc
    19201 cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc
    19261 ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt
    19321 cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt
    19381 gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg
    19441 tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt
    19501 gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc
    19561 tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca
    19621 accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt
    19681 ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga
    19741 tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa
    19801 atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga
    19861 gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa
    19921 aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga
    19981 taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac
    20041 aacaactgac tggcttgcaa tcgatgcttc gagcgagtca attaggcatt tcaccagaag
    20101 aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa
    20161 aaggaaaaat gacagtcgga gaagataact cgacagtcca atacgtcgta agttatgtag
    20221 caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg
    20281 cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc
    20341 atttttacgg aaaattaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg
    20401 gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat
    20461 tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct cttctattca
    20521 tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca
    20581 aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggataca
    20641 tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt
    20701 atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta
    20761 tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct
    20821 tgtctgcaaa catgtacaaa tcgaatgtac tcgtttatag cgaaggcgga ttatggagtt
    20881 cattgaaaca atcgatttca attatgagga atgaacgaaa aaaagcataa aacaatccta
    20941 aaatacagaa ggaagctgga catctattgt tcccagcttc tttttgtata gacctatttt
    21001 atttatttta ctatattgga tatatattca ttgaaaatag tgtataacaa ctttataata
    21061 ttgagtatac caatttttgg aggcgtttta atggaccgtt atttagattt actttcagaa
    21121 aaattcccct caacggagga tgtcatcacg gaaatcatca atctcgaagc aattttgcag
    21181 cttccaaaag gaacagagtt atttttgagt gatatacatg gcgaatttcc tgcttttgat
    21241 catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttttctgaa
    21301 caaatgacgg aaacggatat cagccaattc actattttta cggcttatcc agaatatgca
    21361 ttaacgactg attggtacaa agaacaagat aaatcgcagc tgatccatca tttgatcgat
    21421 cttttacggt ttacgacagt caaatatacc cggtcaaaag ttcgaaaagg attgccgaaa
    21481 gaatatagct atatcattga ggaactcttg tatatcgatg accgtatcaa tggaaaaaaa
    21541 gactatgtaa aaaaaatcat tgaacaactt gttttgatga aggaagaaga aaaattttta
    21601 acgaaacttg cacagacgat ccaaaaatct gtcatcgatc atttgcatat cgttggagat
    21661 atttttgacc gaggaactca agcagccaaa gtgatggatc gaatcatgga tttttcttct
    21721 gtagatatcc aatggggaaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag
    21781 gcatgtttac tgacactttt acggattgca gcacgctata attatttatt tgaattagag
    21841 aaagaatatg ggttgaattt acgtccattg ttttcttttg ctcatcaaac ttatcagaag
    21901 aatccagtat ttgcgcctaa aaacagtaaa aatctatccg aaagagagca agaagaatta
    21961 gaaaaaatcc atcaggcact ttctatcctt caattcaaat cagaacatca gttattggat
    22021 cgtcgacctg aatttaaaat ggacgatcgt aaattgctgg aaaaaatcga ctatgaagca
    22081 tcaacgattg atttagaagg tcagctctat gagttgaaag gaacttgttt tcaaacgatc
    22141 cagccagatg atccaaaaaa attcttggaa gaggaacaaa aagtgattga tagtttgatg
    22201 tattcattcc agcactcttt gagaatgcaa aaacatatga cgtttttgat cgaaaaaggg
    22261 ggaatgtatc tcacgaacaa tcagcatgtg ctgtttcatg gctgtatacc tgtagacgag
    22321 aagggacagc tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctgggc
    22381 ttttttgaaa aacagataac tgaaagcgca aaagatctga ctgcaaaaga agatttagca
    22441 accgatttga tttggtatgc gtggagtggg ccattttcgc ctttattcgg caagagtaaa
    22501 atggcgactt ttgaacgtta ttttctcaca gagcctcata cgcacgtcga gaaaagcaat
    22561 ccttattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttagc agaattcgac
    22621 acaaaagaag aagggtcagc aatcattaat gggcacacgc cagtaaaagt aaaaaaaggg
    22681 gagtcaccga tcaaagcaaa tggaaaagta tttgtcattg acggaggttt atccaaagct
    22741 tatcaaaaaa cgacaggaat tgccggttat tcactattat gcaattctta tggtttccaa
    22801 atcgtgacac attatccgtt tttgcctatt gaacaacaat ttgcaaaaaa atccgaccaa
    22861 acaaatgtga aaaaagtaat cgaacagcaa ctaccacgca aactcaatcg tgatacaaca
    22921 aaaggtatgg aactgcagca gcagattacc cagctcaaaa ggctgttgga ctatcgaaag
    22981 gacgattgag aagaattcca gtgaacattg ttccatagac tggagactga gacaaaagtc
    23041 gtgtcgcagt ctctttttcc ggataaacgg tgttcttctg ccaactcctc ggtcacaagt
    23101 cacactattc aacaaacatg aaaagctgtt ttttgaaatt gctccttgtg actcagagtt
    23161 gaacggcagg ctcacgacct cttggtataa acggtgttca aaagccagtt tctcggtatt
    23221 aagccaaatt tatgcaaaaa tgtgaaaagc cattttcaca aaatttttct taatactcga
    23281 aactaaacag ctttttcaca accccctcag aaagtctatt aaaattcatt cacaaagaaa
    23341 tcgaataagc gctgttcaga tggatgtaca cggtgggtaa gttccggatg ccattgaaca
    23401 ccgaggattt tatgcatatc atctttgctt tgaaccgctt caatgagtcc atcgttagaa
    23461 aaagcgatgg cttttaataa aggtgaaaga tctttgactg cctgatgatg gtaagaattc
    23521 acaaaatatg gatcggggag aaggttgctt aacaaactgt ctgaaacaat cttgacttcg
    23581 tgagtcgtaa attgcggggc agtgggctgt tgttcatgtt ttactgacca ctttggatat
    23641 aatgaaagat cttgataaag tgtaccttcc aaaacgacat tcaatagttg gagtccccgg
    23701 cagactgcaa aaattggttt tctttgtttc aatgcttcca agatcagtgc ctgttcaaat
    23761 tggtctcgtt gaatatttgt ttccgttagc tttggatgag gatcttccat gtaaagttgt
    23821 ggactgacat cttgaccacc tgcaagaata agtttgtcga tttgtccaat cagttgagga
    23881 gctgaatctg gagacatgac tggcagaata agaggcaatc cttgagcatc taaaactgca
    23941 tccacgaatc cttgcggtgt atagctcact tgatttcctt ggaaagtatc tgttgctcga
    24001 atcagttgat ttccggcaat gccgatgatt ggtttcattt gtattccccc taaagtagac
    24061 ttattgtaac aaggatttaa aaaaaattaa actatgtaag acttgcccct atacttatct
    24121 gttcttgctg tgttactatc tgtaaagaga aatttcgtgt gaaattcatg acgatagttg
    24181 aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag
    24241 cttcaaggat ctagttcttc aaggggtgag aaggacattt tattgaaggg gcctgcctat
    24301 actaagttat ctttagaggt acatactttc aatgaaaaaa gaaagcatgt acctcttttt
    24361 taaatcgaaa ggaagtgaag caagtgttag aagctttgga acaaatccga aaagcagaag
    24421 aagaaaatga aaagcgtgct caaacactgc ggcagcagct gaagatctat gcaacagaaa
    24481 aagagcagga attggaaacg atccaaacaa actatcgaca acaactaaaa cagttggtgg
    24541 aagaaaaaga acaatttgaa atacaagaat tagaaaaaga aaaaatgaaa ttgaaagcag
    24601 aagcagacaa ggtgcaagca actttgaaag atcaatatca aaagcatcag agtcaagcga
    24661 tcggtgcgat cattgaaagg gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg
    24721 accctcattt cagataaaag aaatcaggag aggattttac aagcagtaca aggaatccaa
    24781 aacgttgaaa tccgggatct gtatcaaaat agaacaaaca accagtgggt ggagaagtat
    24841 tttccagata ccacgattat tgataaagaa gcaaaattaa gcgctttagg taatcggttg
    24901 aaagacatcc aagaagctat ccaattcata caacaccacg gcgatagccg tcaaaagaaa
    24961 ctccatttga aacgtcaaga actttcctta caagatttgg aagccaatta ttccgaagaa
    25021 aatttcataa ataaattgaa agaagtcctt gatttaaaag aaaagtggga acaactaact
    25081 gaagaacgtg atcgtttaga agaagaggaa gaatggcttc ttcattggca acagctggat
    25141 attgctccaa gtatgtatgc aagtaagacg accatttttg agatggggac tgtgagtgca
    25201 gcaaattttg aaagtttcaa ggaagaactg agtaagcttg atgacgttta tatggaagaa
    25261 atagattata cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa atcagttgct
    25321 gttatagaag aaattgctgg gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa
    25381 aatcctaaag aaaaactgca agagaccaag cagcaattat ccattgttca cgagcagcaa
    25441 aagaagataa cgacagcgtt aggtcagtgc agtggctata tccaagaatt tgaatgggta
    25501 gaagaagtca cacttgccgt tgcagaacga gaaaaaataa aagaacggtt tatccatgcg
    25561 acttatctga tcgttttgca aggttgggta gatgcagaag aaaaacagaa tttgctgcat
    25621 gtgttaaacg aaaccgtatc agaagaagat atatacgtca gtttcgaaac accaaactca
    25681 gaagagattc agcaggaagt ccctactaaa ttgaaaaacc atccgcttgt cgaacctttt
    25741 gaattgctga ctgaaatgta cagtctgcca aaatatgaag aagtagatcc aacaccttgg
    25801 atgacaccat tttatcttgt cttcttcggg atgatggtcg cagatgttgg ttatggtttg
    25861 ctgatgctca ttgggacaat actagctcaa aaactacttg ttctaccacg cgggatgaaa
    25921 cgtttcgtaa aattctttga gattctctcg ataccatcga tcatctgggg attgatctac
    25981 agttcatttt tcggacagtc attaccaaaa gaactgtttg gcatccgctt gcctttccca
    26041 atcctatcta cgacggatga cgtcaatacg attttgattt tatccgtgat tttcggtctg
    26101 atacagattt tggttggatt atttgtggca gcaaaggaaa atatcaggag aaaagattac
    26161 ttgagtgctg tcagtgatgg atttgcctgg caagggatcc taattgggat cgttattgcg
    26221 ttggttggtg ccatgttgat aaaaaataca gcatttgttt accttggcgc tgggctatcg
    26281 atcattgctg ccttatgtat cttgatcgtt ccgattatcc aaacgccgtc taaagtcaaa
    26341 ggtgctgcca aaggtgcgta taatctttac ggactgaccg gctatatcgg tgatttagtc
    26401 agttatacac gtttgatggc attgggtatt tcaggcggaa gtattggagc ggcattcaat
    26461 atgctagttg ctttcatgcc gccagctgct cgattcagtg tagggatcct tttgatcatc
    26521 gctttacacg cactgaatat gtttttgaca ctgctaagtg cctatgtcca tggcgcacga
    26581 ttgcagtatg tcgaattttt tgggaagttt tatacaggag gcggacgtgc ctttgatccg
    26641 ctgaaaacgg cggaaaaata cgtcaacatc aatcacaaga aaaaaaacaa taaataattg
    26701 gaggattttt atataatgat ggattactta attagtcaaa atggtggaat ggtatttgcg
    26761 gtattagcga tggcaacagc gacgattttt tcagggatcg gttcagctaa aggtgttgga
    26821 atgacaggtg aggcagcagc agcattgaca acaagtcaac ccgaaaaatt cgggcaggct
    26881 ctgattctgc aattgcttcc aggtacgcaa ggtttgtatg gattcgttat tgcctttttg
    26941 atttttatca acttaggaaa tgacatgtca gttgtacaag gactgaactt tctaggtgct
    27001 tctctgccga ttgcttttac aggcttgttt tcaggtattg cccaaggaaa agtcgcagcg
    27061 gcaggtattc aaattttagc gaaaaaacct gaacatgcta caaaaggaat catttttgct
    27121 gcgatggttg aaacatacgc gattttaggt ttcgttattt ccttcctatt agtgttgaat
    27181 gcctaagcat aaaggaggaa aagcagatga acgccattga aaaaatcatc tctcagatga
    27241 acgaagcagc agagcaggaa agagcggcgt tagaacaaga agagcgaatg aagatcgatc
    27301 aaaactttga acaaaaacgt acgcaagttg aaacagaaca tcaaaaacaa aaagaaaaac
    27361 aaatcgaatt actcgaaaaa aaataccgtc agctaaggaa tcgccagcaa gtagaagtac
    27421 gacaagaaaa tttgaatgcg aagcaggaat ttttaagacg attgtttgca gatgcggtaa
    27481 ctgaaatgga aaattgggat gaatctgaac aaatccagtt tatcaaaaat gcattgtatt
    27541 cattaccttt gacaggaaaa gtagcattca ttgctggaga aaaatcagca gcctatcttt
    27601 ctcagacgtt gttggatgaa tggaacaatg aacttccctt tatgatggtt ttaagtgatg
    27661 aaactgttgc tgatcaagcg ggttttctga taaacgatca aggagttcag tataattttc
    27721 ttttcagcag ccttgtacaa gatatccaag gaacgatgag ttttgagatt gccaatcaat
    27781 tattcgaata aggagggatc agtgtatgga ataccacgaa ctaaatccct tgattcgcgg
    27841 aagagagctt gaacttttgt ccaatgagac atttgaacgg atgattcaaa caaattctgt
    27901 cgaagcactt ggtgaaatat tgaaatccac gatttacagt ccatatattt atgacggata
    27961 tgaatatgac ttcgaacaga atcttttaaa ggagcagagc cgtctatttc gctggttaaa
    28021 ggaatcagca ccggagcctg aaatcgtttg gatctatacg atgcgttata ctttccataa
    28081 tctgaaggtt ttgacaaaag ctgagatgac aaatcaaaat ctggaccatt tgtatatcga
    28141 cgatggattt tattcattag aaaccttgaa ggatgccatt catacgcaag tttcaaatga
    28201 acttcctgaa agcattatgg acagtatacg agaagtacgt gattattttg aagagtctac
    28261 cattcttcag ggaattgacg tgatttatga tcgacagttt ttgacagagc agcgacggct
    28321 tgccgataag ctaggatatc aagaattgct gacagaagtc attgctttta ttgacttgac
    28381 caatatcaca acgatggctc gaggaatatt gcagaaaaga acatatgggt tcatgacgac
    28441 ggtactttcc agctcgggaa gtatcgaaaa agaagtattt ttgaactttg tagataaaga
    28501 tatggaagat tacacgaatt ttctattaga gacagattat gctgattttc tgcgccctgt
    28561 catccgtgag ggaaatattg atctcgtaaa gcttgaacag ttgaaagatg attatctaag
    28621 ttcattgtat caagaagcac aaacccaggc tttcggacca ctgccactac tagccttttt
    28681 gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg gttatcggca aaagaagcca
    28741 tttcgaatca gaaacaatca gaaagcgggt gagacaagtc tatgacgcat aagatcggtg
    28801 tagttggcga caaagattct gttttgccat tcaaattgtt tggctttgat gttcgttatg
    28861 gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg
    28921 tgatttatgt cacagagcaa tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg
    28981 aacaaatgat acctgctatc gtattgattc ccaatcatca aggatcatta gggatagggc
    29041 tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca aaatatctta tagtatgaaa
    29101 aggagataga aattttgcaa attggaaaaa tcataaaagt ctccggacct cttgtaatgg
    29161 ctgagaacat gtctgatgcc agtatccaag acatgtgtct agtgggagat ctaggagtta
    29221 tcggagaaat tatcgagatg cgtggcgatg tggcgtctat tcaagtatat gaagagactt
    29281 ctggaattgg tccaggcgaa ccggttcgct ctactggtga agctttatct gttgaattag
    29341 gaccagggat catttcacaa atgtttgacg gaatccaacg gccattagat acctttatgg
    29401 aaatcacaca aagtaatttc ttagggcgcg gagttcaatt gcccgcattg gaccatgaga
    29461 aaaaatggtg gttcgaacca acagtagaag ccggagaaac ggtgagtgct ggagatgtta
    29521 tcgggatcgt agaagaaaca aaagttataa aacataaaat catggtgcca aatgggataa
    29581 aaggaacgat caaaacaatc gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca
    29641 ttgaaaccga aaatggtgac aaaaatttga cgatgcttca aaaatggcca gtaagacgta
    29701 gtcggccaat caaagaaaaa ttaaacccgg atgcacccat gatcactgga cagcgagtga
    29761 ttgatacatt cttcccagtg acaaaaggag gagcagcagc tgtacctggt ccttttggtg
    29821 cagggaaaac agtcgttcag catcaaattg caaaatgggc cgatgtggac atggttgtct
    29881 atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat
    29941 tgattgaccc taatacagga gaatcgttga tggaacgtac ggtattgatt gctaatacat
    30001 caaacatgcc agtagcagct cgagaagctt ctatctatac gggaatcaca attgcggaat
    30061 atttccgtga catgggttat gacgttgcta tcatggcaga ctccacttca cgatgggcag
    30121 aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag
    30181 cttatcttgg ttcacggctt gcggaatact atgaacgatc tggaagagtc attgcattag
    30241 gatcagaagg tcgcgaagga agtataaccg caatcagtgc tgtatccccg tctggagggg
    30301 atatttcgga accagtcaca caaaatacat tgcgggtcgt aaaagtattc tggggtcttg
    30361 attccagttt agcccaaaaa agacattttc cttcgatcga ttggatacaa agctattctc
    30421 tttattcaac agaagtaggg aagtatatgg atcatatcct tcaagctgac tgggcatcca
    30481 tggtcaccga gggcatgcgt attttgcagg aagaacagca gctgaatgaa atcgtgcgtt
    30541 tggtagggat tgattccctt tcagataatg accgtctgac attagaagtg gcaaaatcca
    30601 ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt cgatacattc acttcaagag
    30661 agaaacaatt caaaatgttg aagatgatct tgacctttgg tgaagaagga cgccgtgcgt
    30721 tgtcgttagg tgcttattta agtgagatca tggacggtac cgtatcgatt cgtgaaagga
    30781 tcggacgaag caaatatatc ccggaaacag aattagaaaa actagatcaa attattggtg
    30841 aaatcaagca aacgatgcaa acgatcgttt cagaaggagg catgagcaat gattaaagaa
    30901 tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc
    30961 aaatatgaag aattaatcga agtccgtatg caaaatggcg agattcgtcg tggacaagta
    31021 ttggaagtcc aagaagataa agcaatggtc caaatattcg aaggaacaag tggtatcaat
    31081 ttacgtgact cttctgtacg atttttaggc catcctttag aactaggtgt atcggaagac
    31141 atgatcggtc gagtattcga tggtttggga cgaccgaaag ataacggacc agagatcctg
    31201 cctgaaaagc atttggatat taacggagaa gtaatcaatc cagtagctcg ggattatcca
    31261 gatgagttta tccaaacagg tatctctgca atcgatcatt tgaatacatt agttcgtggg
    31321 caaaaattac cagtattttc tggctctggt cttcctcata aagaattagc tgctcagatc
    31381 gcgcgacaag caacggtact cgattcatca gatgatttcg ccgtagtctt tgctgctatt
    31441 ggaatcactt ttgaagaagc tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc
    31501 gaccgttctg tcatgtttat gaatttagca aacgaccctg caatcgaacg tatcgctact
    31561 cctcgtatgg ctttgactgc tgcagaatat cttgcttatg aaaagggaat gcatgttttg
    31621 gttatcatga cagacatgac caactatgca gaggcattgc gagagatttc tgcagcacgg
    31681 cgagaagttc caggaagaag aggatatcct gggtatcttt acacgaactt agctacttta
    31741 ttcgaacgag caggaagaat ccgcggacta aaaggatcgg ttactcaaat tccgattcta
    31801 acaatgccag aagacgataa aactcatcca atccctgact tgactggtta tattacagaa
    31861 ggacagatca tcttgactag agagctttac aagagtggga ttcagccacc aattgatgta
    31921 ctcccgtctc tttcgcggtt aaaagataag ggaactggac ccggaaagac aagagaagac
    31981 catgcagcta cgatgaatca gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa
    32041 ttagccgtag ttttaggtga atctgcttta tctgatgtag acaagatcta tgcgaagttt
    32101 gctgagcggt ttgaaaaaga atatgtcaat caaggattct acacgaaccg ttcgattact
    32161 gaaacacttg atctagggtg ggaactgcta tcgatgttgc caagaaccga attaaaacga
    32221 atcaaagacg acatgctgga caaatatctg ccagaaggga agtgagttct gatggcacga
    32281 ttgaatgtga atccgacccg catggaattg actcgattga aaaaacagtt gacaactgcc
    32341 acaagagggc ataaattatt aaaagataag caagatgaac tgatgcgtca attcatcctg
    32401 ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag agtctatgac agaagcaatg
    32461 tctgattttg tattggcaaa tgcttcgatc acggaacctt tcatcgaaga attatttgtt
    32521 ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa atatcatgag tgtaaaagta
    32581 cctgtgatga attttgaata tgatgaacag ctgacagaca cacctttgga atacgggtat
    32641 ttgaattcca gtgccgaact ggatcggtca atcgatcggt ttactaccct gcttccaaaa
    32701 cttttggaac tgacagaaat cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag
    32761 acacgtcggc gagtcaatgc attagaatat atgacgatcc ctcaattaga agaaacgatc
    32821 tattatattc gtatgaagct ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa
    32881 gtaaaaaata tgggtaccac tgactaagtg gtatcttttt ttaaaagagg aaaacgatga
    32941 ttaaacaaag caaacaaata aagaaacgtt tgccggctgt tcagcttctc gcattaggat
    33001 tttttctcct gattttagtt ggcggcggcc tacttaccct accatttttt agtaatagtg
    33061 ggcatgggac aaattttatc gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg
    33121 gattgacaac attgaataca gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc
    33181 ttttgattga gatcggcggt cttggtttta tgatggttcc gattctatgc tttgcattga
    33241 taaagaagaa agtcagtctc agcacacgta ttatattaaa agaagcgctg aatctagaag
    33301 aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa attggccttt gtgatccaat
    33361 tgattggtac ggttgcttta tcttttgtat ttgtgcctga atttggctgg ttgaaaggta
    33421 tctggtacag tttgttccat gcaatctcta gtttttgtaa tgcagggttt gatttgttag
    33481 gagacagttt agccaatcac cagcaaaatg tttatttgat catggtggta tctgctttga
    33541 tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt actggaatat catcagaaaa
    33601 agaagatgac gattcattca aaaatcgcat taaccgttac aggtaccctt ttattaggcg
    33661 gattcgttgt gttctttttc acagagcgca atggggcaac gctagtggaa gggaatatgt
    33721 ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc tagaacggct ggatattatt
    33781 cagttgatta cctccatatg agccatgcag gtctgatttt aacgatgttt ttgatgtaca
    33841 tcggcggaac atctgggtcg acggccggcg gattgaaaac aactacgtta ggtgtgttgc
    33901 tgatccagat gcgttcgatg ttgaaaggac gtacgagagc ggaagcattt ggccgtacca
    33961 tccgacaagg cgcagtgttg cgggcactta ctttattttt tgttacgttg acgttgtgtg
    34021 tagtcgccat catgatttta tcggtgactg aaacgatacc taaaacttct ggcatcgaat
    34081 atattgcttt tgaggtcttc tctgcgtttg gtacagtagg tcttacgatg ggattgacgc
    34141 cagacttgac tgcaataggg aaattgatca ttatagcgct tatgtacatt ggacgagttg
    34201 gtgtattgac tgtggtcttc tctctgatga caaaagcaca aagaacacaa tcaaaataca
    34261 aatatccaga agaaagtgtc atgattggat aatttcatct tctg
//
LOCUS       DASJST010000012        33538 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJST010000012 DASJST010000000
VERSION     DASJST010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.012"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..1951
                     /locus_tag="U4B05_000434"
     CDS             44..1951
                     /locus_tag="U4B05_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN2049598.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2492..5770)
                     /locus_tag="U4B05_000435"
     CDS             complement(2492..5770)
                     /locus_tag="U4B05_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN2049599.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(6041..6586)
                     /locus_tag="U4B05_000436"
     CDS             complement(6041..6586)
                     /locus_tag="U4B05_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2049600.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(6598..7290)
                     /locus_tag="U4B05_000437"
     CDS             complement(6598..7290)
                     /locus_tag="U4B05_000437"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN2049601.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(7309..7593)
                     /locus_tag="U4B05_000438"
     CDS             complement(7309..7593)
                     /locus_tag="U4B05_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049602.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(7606..8169)
                     /locus_tag="U4B05_000439"
     CDS             complement(7606..8169)
                     /locus_tag="U4B05_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2049603.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            8397..9059
                     /locus_tag="U4B05_000440"
     CDS             8397..9059
                     /locus_tag="U4B05_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN2049604.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            9183..10379
                     /locus_tag="U4B05_000441"
     CDS             9183..10379
                     /locus_tag="U4B05_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN2049605.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(10480..11856)
                     /locus_tag="U4B05_000442"
     CDS             complement(10480..11856)
                     /locus_tag="U4B05_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2049606.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(11893..12759)
                     /locus_tag="U4B05_000443"
     CDS             complement(11893..12759)
                     /locus_tag="U4B05_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN2049607.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(12848..13873)
                     /locus_tag="U4B05_000444"
     CDS             complement(12848..13873)
                     /locus_tag="U4B05_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2049608.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(13891..14565)
                     /locus_tag="U4B05_000445"
     CDS             complement(13891..14565)
                     /locus_tag="U4B05_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2049609.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(14749..15642)
                     /locus_tag="U4B05_000446"
     CDS             complement(14749..15642)
                     /locus_tag="U4B05_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2049610.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(15716..17413)
                     /locus_tag="U4B05_000447"
     CDS             complement(15716..17413)
                     /locus_tag="U4B05_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2049611.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(17406..18719)
                     /locus_tag="U4B05_000448"
     CDS             complement(17406..18719)
                     /locus_tag="U4B05_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2049612.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(18719..22756)
                     /locus_tag="U4B05_000449"
     CDS             complement(18719..22756)
                     /locus_tag="U4B05_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2049613.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(22769..23227)
                     /locus_tag="U4B05_000450"
     CDS             complement(22769..23227)
                     /locus_tag="U4B05_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2049614.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(23525..24043)
                     /locus_tag="U4B05_000451"
     CDS             complement(23525..24043)
                     /locus_tag="U4B05_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2049615.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(24045..24662)
                     /locus_tag="U4B05_000452"
     CDS             complement(24045..24662)
                     /locus_tag="U4B05_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN2049616.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(24659..25513)
                     /locus_tag="U4B05_000453"
     CDS             complement(24659..25513)
                     /locus_tag="U4B05_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2049617.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(25510..26421)
                     /locus_tag="U4B05_000454"
     CDS             complement(25510..26421)
                     /locus_tag="U4B05_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2049618.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(26462..27796)
                     /locus_tag="U4B05_000455"
     CDS             complement(26462..27796)
                     /locus_tag="U4B05_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2049619.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            28004..28834
                     /locus_tag="U4B05_000456"
     CDS             28004..28834
                     /locus_tag="U4B05_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN2049620.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(29078..29396)
                     /locus_tag="U4B05_000457"
                     /pseudo
     CDS             complement(29078..29396)
                     /locus_tag="U4B05_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(29518..29712)
                     /locus_tag="U4B05_000458"
     CDS             complement(29518..29712)
                     /locus_tag="U4B05_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049621.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(30217..30402)
                     /locus_tag="U4B05_000459"
     CDS             complement(30217..30402)
                     /locus_tag="U4B05_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049622.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(31252..31593)
                     /locus_tag="U4B05_000460"
     CDS             complement(31252..31593)
                     /locus_tag="U4B05_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2049623.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(31593..31823)
                     /locus_tag="U4B05_000461"
     CDS             complement(31593..31823)
                     /locus_tag="U4B05_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2049624.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            32110..32448
                     /locus_tag="U4B05_000462"
     CDS             32110..32448
                     /locus_tag="U4B05_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049625.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(32653..32892)
                     /locus_tag="U4B05_000463"
     CDS             complement(32653..32892)
                     /locus_tag="U4B05_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2049626.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(32868..33215)
                     /locus_tag="U4B05_000464"
     CDS             complement(32868..33215)
                     /locus_tag="U4B05_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2049627.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         9927 a         7099 c         5838 g        10674 t
ORIGIN      
        1 gtaagataat gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt
       61 gaaacaagat ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc
      121 agataaacca gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat
      181 gggagaaaca gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca
      241 tttccttgca tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca
      301 accgcttcaa cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa
      361 gcaatttcca gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc
      421 agatatgaag aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc
      481 agataagacg tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt
      541 tgatatggct gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat
      601 cacggaacaa gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga
      661 aaacgcagat tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac
      721 agataaactg gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt
      781 gatcgtaact gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt
      841 tccgttattc aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc
      901 agaagaattc cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc
      961 aatgcctcag aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg
     1021 cgattactac ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt
     1081 gaaaaaaatg atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc
     1141 aacaaaagaa aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga
     1201 taagatccca gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt
     1261 gctttcaaat actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa
     1321 caaaccagtg gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca
     1381 tccatttcgg aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct
     1441 agaacaatca agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc
     1501 ccatgcattt gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg
     1561 gacagaagaa gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga
     1621 tggtctgcct tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc
     1681 tgatgcaggc ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat
     1741 cgaggaattc tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca
     1801 acaactttta ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc
     1861 aaaaaacctt gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct
     1921 agcgccagaa aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa
     1981 gaaataagaa ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt
     2041 ttcgaagaag ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt
     2101 caagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta
     2161 ctaccgagaa actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga
     2221 cctgagtatt agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct
     2281 aataccgagg gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct
     2341 gcatcaagta ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga
     2401 tagaaggaag tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa
     2461 ttgttttaca tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa
     2521 caactacacc gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc
     2581 ccgtttttgg gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat
     2641 gagaaggttt tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg
     2701 gtttgtcatt tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg
     2761 taacagattc tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga
     2821 cttctagcgt atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt
     2881 gagcacttga ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa
     2941 agacaggcgt tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt
     3001 caatgatatt tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat
     3061 tattttgttc atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt
     3121 ccgaagacgg tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca
     3181 ctctttcctt ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga
     3241 tacttccatt ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag
     3301 actcgatcca atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt
     3361 cattccaata gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga
     3421 tctgctctct catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt
     3481 ctgtgtttcc tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg
     3541 tactgctatt gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt
     3601 cgaaagacaa actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga
     3661 atccattacc gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg
     3721 agtttttgat gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac
     3781 cgtcatctgc attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca
     3841 gtttggccgc aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac
     3901 agttcttgac taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga
     3961 tttggattcc tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc
     4021 cagaaatctg taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac
     4081 cccagtagtg actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac
     4141 gcttgttttc tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa
     4201 cgccttttgg aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg
     4261 tctgcccagc atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc
     4321 cgtttcctgc atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt
     4381 agtccactgt atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg
     4441 gcggatagtt tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt
     4501 ttgtctgata atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca
     4561 cctctcctgt tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca
     4621 tagtatgatc ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca
     4681 agcgagtagg tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga
     4741 ttccttctac cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt
     4801 catctgaagc gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg
     4861 tttcaggatc gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca
     4921 gtttagtaat cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat
     4981 aagaacctgt ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg
     5041 agacactccc aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc
     5101 gtgtccccaa tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc
     5161 tatccgagtc ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa
     5221 atgcctgccc aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt
     5281 cttgggcatt cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga
     5341 tgtctttggg gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt
     5401 ttccgccgtt attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc
     5461 tactagcact tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag
     5521 attgcttttt tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg
     5581 tatccatatc ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca
     5641 cgtgtgcatt ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa
     5701 ttaaaaccgg ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt
     5761 tttttaacaa gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct
     5821 aatttatttt tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa
     5881 aaatataact taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt
     5941 ttaaaataac gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta
     6001 ttattttgtg ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta
     6061 gtgtggactc aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg
     6121 aatctttgaa aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca
     6181 cacagatatc tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac
     6241 ggatgtctag atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat
     6301 tttcctcttg ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac
     6361 ccgttcctat cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg
     6421 gatcatgcgc atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat
     6481 gagcagtcag ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct
     6541 ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta
     6601 tacgagatgt ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat
     6661 aaattcgtca aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat
     6721 caaaaatggg atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca
     6781 agctaatgct tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa
     6841 tgtatcacct gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt
     6901 aaccatttca ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg
     6961 tcctgggttg tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat
     7021 aacgatgtca cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac
     7081 catgttcacg ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc
     7141 tataccggaa cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc
     7201 gcctgcgcgt tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc
     7261 ttccattgcg ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc
     7321 gattgctaaa aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa
     7381 agagttccat ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt
     7441 ctttttgctt tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt
     7501 ttctgctttt cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag
     7561 ttcgcttcgc gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt
     7621 caatagttcc cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt
     7681 catcgcttgt tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc
     7741 ttgtgctaaa ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc
     7801 atttgcaaaa cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata
     7861 gccagtttct tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg
     7921 atcgattttt ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt
     7981 gacaagaagc agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa
     8041 taccagttct ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc
     8101 aataattttt ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt
     8161 gttcagcata tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa
     8221 tttttctcaa aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat
     8281 agaaaatcct tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt
     8341 aatcgaataa cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga
     8401 taaaacgaaa ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca
     8461 atagtttttc ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac
     8521 tctttggttc gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac
     8581 ttgaaactca ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa
     8641 caatgaatca aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca
     8701 aacttaaagc aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag
     8761 aattggtgaa agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc
     8821 cgaatctagt tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca
     8881 aacagaccta tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa
     8941 tcgcacaaga ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat
     9001 ctgttgcgaa acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac
     9061 tcttttgttc ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact
     9121 ttttctaaag tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt
     9181 ttatggatca taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag
     9241 atttattgaa caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg
     9301 agattgatca gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg
     9361 aacgccaaga acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg
     9421 tcatcagcta cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga
     9481 atcatgtgca agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc
     9541 gtctccaaga agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg
     9601 tcttcgggaa ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg
     9661 cacaaattga caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata
     9721 atctgatgtt agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt
     9781 atatcgctgc tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga
     9841 tgaaaagagc agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc
     9901 aattcctaga tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga
     9961 tccagcaagc accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga
    10021 tccaagcatc tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga
    10081 ccttgcttcg tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg
    10141 acttattgaa gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa
    10201 atgaacgtgg aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa
    10261 caatccaaga gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag
    10321 tagagcttgg acacatggaa gaagatctga aacaaaaact gttggattta acccaataga
    10381 tcacctttcg cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg
    10441 gcgtttttct tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct
    10501 gtgtccatag ccatagccaa aataaatgat cagtccaata accattacga taccgaacac
    10561 gatccatgtc acagtttgca gacgaagcat caatacaata caagtcaaaa tggagacgat
    10621 tggcaatagc ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt
    10681 tctcaacttc agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt
    10741 tagttccgct aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc
    10801 ggcataagtc gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg
    10861 aagtaagcca tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat
    10921 cgttaccgtc agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg
    10981 tcctacatac ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac
    11041 gattcctgtc aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc
    11101 aatgattcct cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac
    11161 ggcatcaaat cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa
    11221 accaaaaggc atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat
    11281 aaacaacgcg atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt
    11341 cttggcttca agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc
    11401 aatgtatgta ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc
    11461 taaacttttc aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc
    11521 tagtaaaaat tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac
    11581 aacatacaga taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa
    11641 acttagtcca gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg
    11701 accagcattt tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc
    11761 accaattcca agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg
    11821 attgagattg atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt
    11881 ggaaaacagc gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa
    11941 aaggcaacca acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa
    12001 aatcccacct aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa
    12061 accaagtgat ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca
    12121 gatcatgttt ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc
    12181 gcctaagatc attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca
    12241 gttgacgatg atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc
    12301 cttaccaaaa tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc
    12361 agtcaaatac gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt
    12421 cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa
    12481 accagtagac aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc
    12541 attttgtcca acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt
    12601 gattacagga ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat
    12661 tcctagtgtt tgttgattgg catcgccacc aattttacca cttactaaac caatactacc
    12721 ccatgccaac atagggacta atgctattaa aatttccata accaatctcc ttttatttca
    12781 catgataaga aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa
    12841 cgattttcta acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg
    12901 tcccttttcc ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt
    12961 ttgctaaata aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag
    13021 taaatccctt atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt
    13081 taactgaaag gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat
    13141 ccggcgtata tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag
    13201 catcggtcaa aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa
    13261 aataatttgc ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga
    13321 tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt
    13381 catcgatttt tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact
    13441 cgatcgaacg aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg
    13501 agtcaatata atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt
    13561 gttcacggat cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac
    13621 cactcaaata attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc
    13681 ttctcttgct caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag
    13741 ctaaataagc aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga
    13801 tcgtcaatgc aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt
    13861 taaaaaaatt catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt
    13921 tgtttcgata tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt
    13981 gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt
    14041 ttcccgactt aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt
    14101 tttgctcaaa tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt
    14161 aatgccattg tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag
    14221 taatgcattg atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc
    14281 tgcccccatg ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat
    14341 gatcggtact ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg
    14401 aagattaata tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg
    14461 attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa
    14521 ttgtcgaatg atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat
    14581 ctttcttctt gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga
    14641 aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc
    14701 tcttccaagc agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt
    14761 tttctttcgt tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag
    14821 cactagcaat atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg
    14881 ttctatcttt gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg
    14941 gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct
    15001 aattgctaat gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc
    15061 gagcatccga ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc
    15121 cgtaaagagt tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc
    15181 tcctccagtt ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc
    15241 attcgtttca tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat
    15301 catatgttgt tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa
    15361 aggatctttg acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt
    15421 cttttcatgc actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt
    15481 ttgataattc tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg
    15541 atatgtaaaa actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag
    15601 actgatttgg gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt
    15661 taaaaaaact gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata
    15721 cttcagactt cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc
    15781 cgattagtaa gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca
    15841 tattcggtac tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat
    15901 agcttccata agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac
    15961 cttctggcgc catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa
    16021 agtatccatt tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc
    16081 tatctgctcc tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt
    16141 cagaacggtc ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg
    16201 catcatattt ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt
    16261 cttcataatc aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat
    16321 catccgcttc tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac
    16381 gtgcatctgc taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa
    16441 tcgtcacgac aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa
    16501 tcgtctcgcc ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg
    16561 tatctgtgat tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga
    16621 aataaggaat aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat
    16681 aagaaatcgt ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt
    16741 cctctacatt catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat
    16801 ctgtattgta tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca
    16861 tcatcactgg catcataaca accattggag acgcgattct ggaaatatga gctggcgaat
    16921 aagtttctaa gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga
    16981 cggtagagaa taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc
    17041 gatattttaa aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag
    17101 aattaagacc gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata
    17161 cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg
    17221 tagaaaccaa tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta
    17281 atgtaatcgt cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt
    17341 tcccgaaaat ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca
    17401 tttttttatt catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat
    17461 aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac
    17521 cattttcctg attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt
    17581 tcttttacgg tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact
    17641 ttcccggatt ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct
    17701 tttgcatcca cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc
    17761 tcgtaacctt ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt
    17821 tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc
    17881 aaaggatcat tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata
    17941 tctaaataat atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat
    18001 ggttttcctt tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt
    18061 agataaccga catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc
    18121 gggtgataaa ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc
    18181 aatccaactt ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg
    18241 tttttcttag gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc
    18301 ccagttgtta ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata
    18361 ttgtttctgt tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca
    18421 gctttttttt catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt
    18481 ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca
    18541 tcattttcat ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc
    18601 aacacgaatt ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg
    18661 actgctgcaa gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt
    18721 accttgctcc ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg
    18781 ccatgaagaa tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca
    18841 acggattttc aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa
    18901 gttcatattc taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg
    18961 aaatctcaac ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt
    19021 caggttcttc tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt
    19081 caggtgactt cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata
    19141 gggaagaatc gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac
    19201 tattttgttt ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa
    19261 ttttcaaaag ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac
    19321 catcgatgat ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt
    19381 caatgataaa cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat
    19441 acccatttgg tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt
    19501 ggtcgtaatt taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga
    19561 tcccaggcaa acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca
    19621 gcgggaagga agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc
    19681 cttcatggtc taattttcta actcggatct cccgataatc aaacgtattg taagcagtga
    19741 atgctaccca taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt
    19801 cacctgtttg cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg
    19861 tgtcattttc atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt
    19921 ctgcatagtt ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg
    19981 gatgaataac tttctcacca tccacgatta catatttccc gtcttcatct actgaataga
    20041 acgtgagtct ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg
    20101 gatatccgat ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga
    20161 ttggcatatt actgccttca attagtttat tgatattcat gtggattggc ttgaattcat
    20221 ttcgcatttc cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt
    20281 gtacaacaga aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg
    20341 gtccgccgac tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct
    20401 catcttcgtt ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat
    20461 aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat
    20521 ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg
    20581 tttcgattgg ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc
    20641 cagccaacgg ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt
    20701 cgtaacgaat ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat
    20761 cctccaggtt ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc
    20821 ccgcatcaag ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg
    20881 gagaaaattt ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt
    20941 ctggtggggc ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc
    21001 cgttttctcc tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg
    21061 gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg
    21121 tagaagagat atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc
    21181 gcggtgttac gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag
    21241 catctagtcg ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac
    21301 ttggcgggtt ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg
    21361 cagatgactt ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac
    21421 caacccagtg agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat
    21481 ttacttcatc ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg
    21541 cattagaagt attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag
    21601 caatcttatc ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac
    21661 ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt
    21721 tttctggatt tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa
    21781 gccctagtgt aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc
    21841 ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt
    21901 tattgatagc tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg
    21961 ggtcagagag cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag
    22021 tttgactatt ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa
    22081 cgatatcgat tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt
    22141 aaagggttgt tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt
    22201 ctacccattt tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt
    22261 catttcccca aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg
    22321 atccattatt agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac
    22381 tatcatcgat caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat
    22441 tgctttgtgt ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc
    22501 gatgctgttc gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag
    22561 ctgttggccc aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg
    22621 cttgaccgct gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt
    22681 gaatttcagc ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc
    22741 tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg
    22801 tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt
    22861 cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac
    22921 ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac
    22981 gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt
    23041 cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat
    23101 cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact
    23161 ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc
    23221 ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa
    23281 caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt
    23341 tagttaaaaa acaccaatta tacaacattt attattaaaa ttaaattata aaattttata
    23401 acgttctcat ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc
    23461 tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat
    23521 taattcagat aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta
    23581 aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa
    23641 ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa
    23701 aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga
    23761 caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca
    23821 aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat
    23881 caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat
    23941 tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact
    24001 tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta
    24061 agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg
    24121 aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac
    24181 gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta
    24241 atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg
    24301 cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa
    24361 tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct
    24421 tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga accgagcttt
    24481 ttctcttcat tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct
    24541 aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga
    24601 atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc
    24661 atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga
    24721 acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg
    24781 tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt
    24841 ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa
    24901 tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat
    24961 tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac
    25021 gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag
    25081 tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg
    25141 ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct
    25201 tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag
    25261 tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat
    25321 agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca
    25381 ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta
    25441 ctgtcatgag gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga
    25501 aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa
    25561 attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata
    25621 acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga
    25681 atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa
    25741 agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt
    25801 aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga
    25861 tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat
    25921 tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg
    25981 gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat
    26041 ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa
    26101 tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt
    26161 cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa
    26221 atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt
    26281 caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac
    26341 caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt
    26401 tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg
    26461 attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt
    26521 ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat
    26581 ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt
    26641 gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg
    26701 tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag
    26761 aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca
    26821 agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca
    26881 tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat
    26941 tttgggcacg catggctact aatccggaaa taaatggatc agatacacca gacccaaatt
    27001 cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact
    27061 ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac
    27121 tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa
    27181 aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta
    27241 attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt
    27301 tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat
    27361 attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt
    27421 tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta
    27481 caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac
    27541 cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa
    27601 tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat
    27661 tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg
    27721 cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa
    27781 acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg gttttaatat
    27841 aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt
    27901 aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt
    27961 agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa
    28021 agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca
    28081 ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg
    28141 ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga
    28201 aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt
    28261 tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat
    28321 gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa
    28381 tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa
    28441 tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga
    28501 tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct
    28561 atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac
    28621 tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg
    28681 agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata
    28741 tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc
    28801 aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt
    28861 tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca
    28921 aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg
    28981 gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag
    29041 gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat
    29101 ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt
    29161 gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt
    29221 ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt
    29281 atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg
    29341 gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc
    29401 tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag
    29461 aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta
    29521 tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata
    29581 aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact
    29641 agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag
    29701 tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt
    29761 actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc
    29821 aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat
    29881 ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata cataaccttg
    29941 atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct
    30001 aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa
    30061 gtatctgaac tattaatttt taaactatca tttttatcct caactacatg cacatctgtt
    30121 gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct
    30181 tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc
    30241 aacagcaaaa aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga
    30301 aaaattcagc aactcattac tgtatgattt tagcagccca accaacatat accctataaa
    30361 agctaataag atggttagga atcctttaga atttttactc attcatagac acttccttaa
    30421 ataactttta ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata
    30481 aaaaaaacct tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact
    30541 gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta
    30601 gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac
    30661 tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta
    30721 gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga
    30781 tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt
    30841 tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta
    30901 cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt
    30961 tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact
    31021 tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg
    31081 acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata
    31141 cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt
    31201 aatttcgtta tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat
    31261 atactgtata atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt
    31321 gagttttcgt tccttaaaat caagagattt taattgtgta ataattactt gtccttttat
    31381 atctagctct tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg
    31441 acatatgacc gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg
    31501 tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc
    31561 ttttttagga atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt
    31621 aaatcttccc attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct
    31681 tcaatttttg gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg
    31741 ttttctgaag gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt
    31801 aatctagatt ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca
    31861 ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata
    31921 tcttattgga tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa
    31981 ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc
    32041 cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc
    32101 catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt
    32161 caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa
    32221 tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt
    32281 gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat
    32341 atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac aactgccaac
    32401 cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg
    32461 ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta
    32521 gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt
    32581 ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa
    32641 aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag taatgatttt
    32701 tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg
    32761 cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc
    32821 tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt
    32881 atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac acaacgattt
    32941 tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg ggcattaatc
    33001 atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg agtgaggatg
    33061 ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc tccagctgct
    33121 aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt ctgaaggagc
    33181 tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa tctttttttc
    33241 tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt tgtagaacta
    33301 aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc aactcctcgg
    33361 tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt tccttgtgac
    33421 tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag ccagtttctc
    33481 ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat ttttctta
//
LOCUS       DASJST010000013        33353 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJST010000013 DASJST010000000
VERSION     DASJST010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.013"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..485
                     /locus_tag="U4B05_000465"
     CDS             <1..485
                     /locus_tag="U4B05_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2049628.1"
                     /translation="FNKNTVRLKYALDKYQRKTHTKGWKDTVIQAPEVVMTLLREWKS
                     AQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRINAIKKRHPELAQLSPH
                     KLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVVDKSVFEAFQKGLNKCD
                     "
     gene            complement(574..966)
                     /gene="rpsI"
                     /locus_tag="U4B05_000466"
     CDS             complement(574..966)
                     /gene="rpsI"
                     /locus_tag="U4B05_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2049629.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(982..1425)
                     /gene="rplM"
                     /locus_tag="U4B05_000467"
     CDS             complement(982..1425)
                     /gene="rplM"
                     /locus_tag="U4B05_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN2049630.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(1944..2411)
                     /locus_tag="U4B05_000468"
     CDS             complement(1944..2411)
                     /locus_tag="U4B05_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN2049631.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2531..4069)
                     /locus_tag="U4B05_000469"
     CDS             complement(2531..4069)
                     /locus_tag="U4B05_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN2049632.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4149..4835
                     /locus_tag="U4B05_000470"
     CDS             4149..4835
                     /locus_tag="U4B05_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN2049633.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5036..5563)
                     /gene="msrA"
                     /locus_tag="U4B05_000471"
     CDS             complement(5036..5563)
                     /gene="msrA"
                     /locus_tag="U4B05_000471"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2049634.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5647..6324)
                     /gene="pgmB"
                     /locus_tag="U4B05_000472"
     CDS             complement(5647..6324)
                     /gene="pgmB"
                     /locus_tag="U4B05_000472"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2049635.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6321..7449)
                     /locus_tag="U4B05_000473"
                     /pseudo
     CDS             complement(6321..7449)
                     /locus_tag="U4B05_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(7449..9632)
                     /locus_tag="U4B05_000474"
     CDS             complement(7449..9632)
                     /locus_tag="U4B05_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2049636.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9629..10654)
                     /locus_tag="U4B05_000475"
     CDS             complement(9629..10654)
                     /locus_tag="U4B05_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2049637.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10699..11526)
                     /locus_tag="U4B05_000476"
     CDS             complement(10699..11526)
                     /locus_tag="U4B05_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2049638.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11523..12590)
                     /locus_tag="U4B05_000477"
     CDS             complement(11523..12590)
                     /locus_tag="U4B05_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN2049639.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12616..13452)
                     /locus_tag="U4B05_000478"
     CDS             complement(12616..13452)
                     /locus_tag="U4B05_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2049640.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13449..14318)
                     /locus_tag="U4B05_000479"
     CDS             complement(13449..14318)
                     /locus_tag="U4B05_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2049641.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14388..15671)
                     /locus_tag="U4B05_000480"
     CDS             complement(14388..15671)
                     /locus_tag="U4B05_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2049642.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15685..17370)
                     /locus_tag="U4B05_000481"
     CDS             complement(15685..17370)
                     /locus_tag="U4B05_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN2049643.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17645..18670
                     /locus_tag="U4B05_000482"
     CDS             17645..18670
                     /locus_tag="U4B05_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2049644.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19265..22939)
                     /gene="rpoC"
                     /locus_tag="U4B05_000483"
     CDS             complement(19265..22939)
                     /gene="rpoC"
                     /locus_tag="U4B05_000483"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN2049645.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(22997..26614)
                     /gene="rpoB"
                     /locus_tag="U4B05_000484"
     CDS             complement(22997..26614)
                     /gene="rpoB"
                     /locus_tag="U4B05_000484"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2049646.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27342..28310
                     /locus_tag="U4B05_000485"
     CDS             27342..28310
                     /locus_tag="U4B05_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2049647.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28328..29233
                     /locus_tag="U4B05_000486"
     CDS             28328..29233
                     /locus_tag="U4B05_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049648.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29230..30036
                     /locus_tag="U4B05_000487"
     CDS             29230..30036
                     /locus_tag="U4B05_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2049649.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30172..31161
                     /locus_tag="U4B05_000488"
     CDS             30172..31161
                     /locus_tag="U4B05_000488"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN2049650.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31478..32002)
                     /locus_tag="U4B05_000489"
     CDS             complement(31478..32002)
                     /locus_tag="U4B05_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN2049651.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32289..>33353
                     /gene="scm"
                     /locus_tag="U4B05_000490"
     CDS             32289..>33353
                     /gene="scm"
                     /locus_tag="U4B05_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN2049652.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         9408 a         7162 c         6267 g        10516 t
ORIGIN      
        1 attttaataa aaatacggtt cgtctcaaat atgctttaga taaatatcaa agaaagacac
       61 atacaaaagg ttggaaagac accgttattc aggcaccaga agttgtgatg acattattaa
      121 gagaatggaa atcagctcag gctgaacaat tgttgaaact aaaaattata caaacacctg
      181 accagtacct ctttacctat tccaaacctt ctggagaagt aaattgtcct gttcacgctg
      241 actacttaaa ctatcgaatc aatgcaataa aaaaaaggca tcctgaattg gctcaattat
      301 ctccacacaa acttcgtcat acctatgcaa ctatagctag ggaaggtgga gcaaatatga
      361 atcaaatctc taatgcttta acgcactcgg atatttctac tacaaaaata tacgtgaaca
      421 cacctgacgt agttgataaa tctgtttttg aagcatttca aaaaggattg aataagtgtg
      481 attaacatgt gttctcaaat gtgttcttat tataaaaaaa gacactttcc aaataattga
      541 aaagtgtcct gaaatattga tataaaaaga atattaacgt tttgagaatt gtgaagcttt
      601 acgagctttt ttaagacctg gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa
      661 gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg cacgagcgat
      721 accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt taacaaatac
      781 gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt cacgcaagtc
      841 agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc cagttcctgg
      901 tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat attgaacttg
      961 tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt ctggttgttg
     1021 tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc
     1081 taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac gagaattttt
     1141 agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc tgtgacggta
     1201 ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat tgatgacgat
     1261 gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac cacgtagtac
     1321 agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta ctacatacca
     1381 tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat tttcctccaa
     1441 tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg gggcaaacaa
     1501 taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg cttgattgaa
     1561 aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg tttcctgtca
     1621 aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta ttttgaataa
     1681 acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa cataagaagc
     1741 tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca acctcttggt
     1801 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     1861 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt
     1921 tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc caaatgagct
     1981 tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat atcttgtgtt
     2041 ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc tgctagataa
     2101 cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca gcggccaatt
     2161 tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg tgaaccgtct
     2221 aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc catgaactca
     2281 tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg tgtttggtga
     2341 acaacaacag acatttggct tagatctgca actaattgat ttaatacttc tttggtttgt
     2401 tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat agtagacttt
     2461 caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc ttttttatcc
     2521 gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca aattgctgat
     2581 aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc tgatattttt
     2641 tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc tgtgcttcac
     2701 tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct aatgcggatg
     2761 cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc atctgacgaa
     2821 aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga ttttcctggc
     2881 aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc gccttcatca
     2941 tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca tctgcgtccc
     3001 aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg gcacccgctg
     3061 gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt tctttagcag
     3121 attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc
     3181 tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac gtctccattt
     3241 ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt cctacggtca
     3301 aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc ccgtctgccc
     3361 ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt tttgatggta
     3421 gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca
     3481 agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat ccagttcctg
     3541 aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata tagtctttga
     3601 taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc agccacttga
     3661 ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa aattctttgc
     3721 caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct gcccaagtga
     3781 tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc atcgcagagg
     3841 agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact tcttttataa
     3901 cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct tgtgtttctt
     3961 ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa
     4021 ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt ttttcacctc
     4081 aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt tgaaaagggg
     4141 gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt ttatgtttaa
     4201 ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat tgtgtcaaat
     4261 gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc gtccaacgat
     4321 ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca gaagccttat
     4381 gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt tcgacgttca
     4441 tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc atggtccatt
     4501 ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag gccggcgtct
     4561 tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc
     4621 aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt ggcttgtctc
     4681 tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc gttttttctg
     4741 tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct tttttgagca
     4801 gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca aacgacttgt
     4861 cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag ctaggcgaca
     4921 ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat tagctcaaaa
     4981 tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg gcttcttatt
     5041 tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct cgataaagat
     5101 tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca ggataaaatt
     5161 cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg
     5221 cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag atcactggac
     5281 gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc
     5341 aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg atctctactg
     5401 cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt ggtacatgcc
     5461 cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat ggtttgacca
     5521 tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat cccccttttt
     5581 tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg
     5641 aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta cttgatcaaa
     5701 aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt cacctattcc
     5761 caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat cttcaatgac
     5821 caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa cttctggatc
     5881 aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt
     5941 cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga tcttgatgtt
     6001 tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg tttcatccat
     6061 tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg atttctcttt
     6121 ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg actcggttct
     6181 ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca gctggttagc
     6241 taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc catctaaatc
     6301 gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa
     6361 gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt
     6421 aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc tgctgctttt
     6481 tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc tggtactctt
     6541 ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg tccttcgcct
     6601 acgattacga ggtctgcttg ctgtacccgt atcgaactcc agtaaatcca atacgtagtc
     6661 gatgccagag actattttcc ctgaagcaaa tgctaacagt cctgcaccca ttccaccacc
     6721 agcactactg cccgatgcct cgattacttg aggggcaaat ctcatataaa aatcatgcat
     6781 ggcttggtca gccactgcta attcattttc tttcaagcct ttttgtggtc cgaaaacaaa
     6841 agaagcgcct tcttttccgc ataaaagatt agtaacatca gtaatgacag tgattttggc
     6901 tttaatcagc tcaataggca cgttcgtttt atcaactgta gcaattttac ctagattttc
     6961 tcccagtgct tctacttttt gattatcttg atcgtaaaaa gcataaccta gaccttctgc
     7021 catgccgatg ccgccatcgt tggtactact tccgccaacg ccgatgatga tgtgtcgaac
     7081 accttgttgc aacaattgtc gtatcagttc tcctactcct cgcgtggata acctaagtgg
     7141 ctgttgttgg tttagtggga catctttcaa tccgacgatt tctgccattt caaaaatcgc
     7201 agtttgaccg tctgaagcgt actggatttc tttgtttttg ccaaatgcat tagtaacgac
     7261 tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat gaaaccgttc cttctcctcc
     7321 atcgcctata ggtagcagat catattcagc ctgaacaaaa acttcttgaa aacctgattg
     7381 aatggcttgt gctgcctcta aggctgtcag actttctttg aaagaatctg gcgcaatcag
     7441 aatacgcatt attttcccct ccgttccaag atcagttctt tttccagctg gtattggtct
     7501 ccattgacaa aaataggtaa tgtttctttc gtctcttttt ttattactat ttgttttttt
     7561 gtcaaaacaa atagcaacct gtgatttttc caaataaaag gaaaagaaat tttttcccat
     7621 gctgaaggaa gtcttggaga aatagaaaga gttccattcg ttactgtaat acctgaaaaa
     7681 ccaaatataa ctgctagcca tagtgctcct aatgaagcag catgaattcc gtcatctgat
     7741 gaatgcggat ctggtcctaa atcaatcagg cacgcttctt ggaataaatg atacgcccat
     7801 tccatttctt ggatacggca agcctctatg gcgtgaatcg ctttactcaa cgatgagtcg
     7861 tggattgttt tgttttcata gtaatgcaaa ttcttagaaa cgattggttg agaaaaatac
     7921 tcaggtaata agtataacag catgacaaga tctgcttgct tgctgacttg gagttcattt
     7981 acttcgctac gggcataatc aagtaaaata gactggctgc caggacgttc tttgtaagtg
     8041 gtcaaatcaa taactggctt actgagatac gtgtcatctt gcggaatcag ctgttctttg
     8101 ttaggcactg gcaaatataa cttatctaaa aaattttgac attctttcaa aaaaagcgga
     8161 tcgccagctt gatatttaac agctaaggcc acattataat gcgccagata gttcgtatat
     8221 gcattattgt cgatatattc tgtgtattca tctggaccaa tgactctgtg tatctctaaa
     8281 tgatcatcga cttttacagc tctgctcagc caaaaagctg ctgtttcatg aatgagcgtt
     8341 tttccctttg tttctaaaaa cgattgatcc tctgttgctt ggacatactc ttgaacggca
     8401 taagcaaggt ctgcactgat atgatgttct gcggcagctg aagcaacttc ttgtcttgtt
     8461 ccggttttga tattcaatgc agcaaatttg ggtgtctctt cctgaccagt gtgtgcgctt
     8521 tcccatggga aaagtgcacc ttgatagtga taatctcttg ctttttgccg tgcgccttct
     8581 aatcgatcat aacggtacag cagcaaattc tttgctactt caggaaactg atataaaaag
     8641 aaaggcagga taaaaatttc cgtatcccag aaaacatgcc ctttataacc ttcgccagtt
     8701 agtcccttcg cccctacact aacacttgga tcattgcggg gagtcataat ctgtaaatgg
     8761 taagcagcga agtccatcgc taactgatcg aaagaagaat cgccttggat cgttatccga
     8821 tgctgatgcc aaaaatctgt ccaataagac atcgactgct ctaaaatttc tgtataacta
     8881 acgcttttct gggcttgggc aatacttttc ttaaacgggt ctgtttgctg aagagaggta
     8941 gagagcacga tgatcttctc cagcgtaaag gacgtatttt ttctgatctt ttctgaaaag
     9001 gaagcggtga tccggcgatt ttttgcttgg aatacacctg ctttattgaa tttagctgca
     9061 atcccaatat caattttact ttctgttgtc tggtaggatg cactgatcaa atcgtcacca
     9121 aatactcgca gttctttttc aaccaactgc tgtgtaccaa aattggtttg ctgtccatcg
     9181 atacccgttt tcacatctac ttggcagtct tcggtcaacg gcttgatgac cactttcagg
     9241 gctgcctgat ggagattatt ttgatcagca acacgacaag taatgacttc cacttttttt
     9301 ccttgagcag tttcaatgag cgcatagcgt ttaagctctc ccatttctaa atctagtgcc
     9361 cgctcgtatg caagaacttt catcgtctca agagaaaaat gttcaccgtc gatttttatt
     9421 tcccatctga tgacatctgg gagattcact aactcggctg attctgctcc taaaggtcgg
     9481 ttatatacac cagctgcaaa cattcccctt acttgattcc gatagtcttc ctcctggctt
     9541 cctctgatac caaaaaatcc atttccaatg gtcatctgac tcgcatactt attgatatac
     9601 tctaaatcaa actgtttttc ttctactttc ataaggtaat aacttctcct gtttctgccg
     9661 aacgatataa attttccacc agattctgga tgacatatcc ttgccagcca tctgcaatca
     9721 tctgagtctg gtcaccgagc gcccgattca caaaatgagc catacttagt tcatgtgggt
     9781 cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc atctacatct tcgaatagtt
     9841 cgcctggata cagattagca cctgcctgat cgccacaaaa agccacattc agtttgtctt
     9901 ctctgatatt caaagcaaac gaagtattca gttggatgat cttgccgttt tctaattcca
     9961 gtgtgccaaa aattgcatct tctactgtga atttgcttgg atcccatttc ccgaatttcc
    10021 ctgaattttt tttcgtacca attttttgga agctgtacgc ccgaacgctt tttactttag
    10081 gaaatccagt aatatacatc gctgtatcaa gcatgtgaat gccataatcg atcaaaggcc
    10141 cgcctccttg aagttctttg tcgataaacg ttccccagcc tggaacacca gaccgacgaa
    10201 gcgcattcgc ttctacgtaa taaatatccc cgagaacatc aagatgattc ttgatcattg
    10261 ttgcttcgtc agaaaaacga tgctggaatc cataagccaa aatctgcccg tttttatcgg
    10321 ctgtctctgc catctttctt gcttgatcag ctgtcatagc tggtggtttc tcgcacatca
    10381 ctgaagcatt agcttcgagg gcagccaata catgttcgaa gtgaaagcga ttggctgtac
    10441 agacactcac gatatcggga gaaatctcat tcagcatttg tgtaactgat gtatacgcat
    10501 gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg gctgactgga tcaaccacag
    10561 cggccaattc taagacatct gatctttttt gataataagc agcatgtact ttttctgcta
    10621 cttgtccagc gcctatgatt gctactttct tcatttattt ctctctcctt tgcttattta
    10681 cttattgtta ttctttctct attctttctc taaatcattt aaatgacgcg ctgtttccag
    10741 acagtttttc aagtattgca atgattcctg ataaattttc acttcatttt ctcctcgcaa
    10801 acgacattca tataccatat aaccttcata ctcattttga agtagcgttt gaaaatggcg
    10861 agtaaaatca attgcgccag atcctggttg gtagcgatga ttatctgcta aatggatatg
    10921 acccacatac tctttttgcg tagtcaatgc ttttgaaata tcatcttctt caatattcat
    10981 atgataaaaa tcagcgatga tctgcacatg tttcaattga ttagagtcaa tgtaatgacg
    11041 cgcatccgct aacaaattga tcatatggtc ttggtatcgg ttcaaaggct ccaaataaat
    11101 aaccgtacct gtttcttcgg ctactttatc taaataacga agtgattcgc tgactgcttt
    11161 atagtctcct tctttgcttc ggggtgaaac catcggcggt aatcgataag tgaacattcc
    11221 ccaagctgct gggataacga ttcctttgcc tccaacctcg cttagtgcac gcaaaatctc
    11281 agcaatttga cgtacaccat taagacgccg ttcttcgata aagtcaccga tccacccatc
    11341 gtagccgtta caagctgtca ctactgggat ccctgtttct ttgattgcct gtttgactgt
    11401 gtcgatattt tctattaaca acttaccgtc gatttcgtat tcttcaaatc cacagtcttt
    11461 gataaatcta aatttttcta aaatgtctct tgggaaaaat gcttgatctt gcgtaccgaa
    11521 tttcattgtt ctcctcctcg gacttgaacg cccattttga tactttcttc tgggtgctgg
    11581 tctacataac gcatgtaagc ttctgcgctt tctgcaaatg gcacaactgg atcaatgatt
    11641 tcttcacaat ccaagtaacc gttcatcaat aattcccaac atgtgtcctc gattcgttta
    11701 cggttccatc ttgggtaatc tgggtttggt tcactagatg ctcgagcaaa gacgattttt
    11761 gcattattga aatgtgcttc gcgaccaaga ttaaacccag ctggaaaagg tttagcaaag
    11821 gcaacataag aaatgatccc gccatagccg atccctctta aagaagcttg taatgcggca
    11881 gcactgccac tcgtttcaat aactgaatct actccacgtt tatctgtcaa ttcctttaga
    11941 cgaaaaccga catctgcttt cataggatct agcgtgtaat cggcaccgta tttttctgct
    12001 aattctctac gctgcgctat gggatcgatc ccaacgatgg tagtagcacc tgctttttta
    12061 gctaattgaa tcgcaatttg cccgatagcg cctaaaccga agattgctac agcatcacct
    12121 gctcgaacat gtgcttcccg gataccacct aaagcgaatt gagcaggatc gtaacatact
    12181 gcatttttcc attgatccaa ccgatcaagt ttacgtagtt tataattatc aacaccgttt
    12241 gtaatcagcg tttcacgaat gggtccataa gtgcagacta actctccttt ttgatactct
    12301 gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca tatttcctaa ttgaaattcg
    12361 ccaaatacca caccgcgttg gcttccttct tctcttggca agaataggcg ccattcttct
    12421 gaaaattctt catcgataaa tggacttaat cctctaaagt cgacaatttc tgttcctggt
    12481 tttggagtag cgaaaattac tcggatacgt acttcgttct tttcgatctc acggtcttcg
    12541 taatctttga atccggctat tcttggttct aatgcaacta attttttcat cttttctttc
    12601 ctctttcttt tctttttatc cttttacgcc gccttcgact aaatcacctt taataaatcg
    12661 ttcagataac gcatacataa tgatgactgg taaagcggtc accaatgaag cggccatcat
    12721 tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc aaaccgattg gtaaagtaaa
    12781 tttttcggag gtcgacagga aaatcgaagc aaataaataa tcattccacg aaaccataaa
    12841 gcaataaacg aagacagaaa tgattccaga aatagccaat ggtataatga tccgaataat
    12901 gatctggatc cggctaagac catcgatcat tgctgcttct tcaatatcag tcggaatcgt
    12961 gtcaaaataa ctttttagca tgaatatcgc agtaggaagt gtttgaacga ccatcgtgat
    13021 aatcagtgct gtattggtat catacaaacc tagaccggaa atgattttaa ataatggaac
    13081 aacgatcaaa ataccggaaa acatataaac ggtgtaaaag ctagcattga ttttcgagcg
    13141 agctttgaag cgtagttttg acaaagcgta tgccccgctg atcccaatca ctactgcagt
    13201 tacggaggct atcaaggaga tcactaaact atttttgaaa tacatgatga atggaaaaat
    13261 ctcagcattg aaaatatcct gataatgttc aaaagtccat tctttcggaa aaatagtttg
    13321 tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc atcagcatga tcaaaaatgg
    13381 aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa aataagattt tcctgctctt
    13441 tttatttttc atttcaagtc caccttcttt cgtgagagca agacgaccgc tgcaataatg
    13501 atgaacaata gaatcgatat agctgctgct tttccaagat cattgaacgc aaaggctgtc
    13561 tgatacaaat aaacgcctaa gatattgact ttattcgtca gtagatagac atctgtaaac
    13621 atatagaaca tccagataga tcgtaaaaca atcacagttg ctagtactgg tttgattgcc
    13681 ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac cgtccatttc agctgcttca
    13741 taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg aaataaacgc atacggatag
    13801 tatctccaga tagcaaacaa aacgaccata aagaagctag caactggctg gtcgaaccac
    13861 agcggtgctt cgttgaaaag ctgcaaccta tctactaaca gatcattgac gattccataa
    13921 ctattattga acatatattt ccaagcaaaa atcaaagaaa tagatggcgt aacataactt
    13981 aataaaatca gtgaacgcac cgttttccta aatctgaact tgcggttaag caaaatagct
    14041 acggctaatc ctaaagctgt actgcctgct acaactaaga atgtgtaagc aactgttaaa
    14101 cctaaagaac gataaaattc actatcactt aaaatttcaa gataattgct taatccgata
    14161 aaaacggcgt ctgagttagg attcagcggc atatcgaaaa aactgatctg aatattggaa
    14221 agcattggaa tcagcactaa aacagctaat aaaagcatgc ttggtgctaa aagtcccatg
    14281 gctaatttta agtcagaatg tttccttgga cgattcattg aaacctcccc ctgataatct
    14341 gccaatagct atctactttt ttcgaacacc attggcagaa cttcttgtta tttcgtattt
    14401 ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc cgacagttac ttggttcact
    14461 gcttttccga tcacacctga gcttgtgata tctcccattt tcgtgaaatt cttttccccg
    14521 actagtccaa agacttgtac ttcatcaaaa gcactagcaa tctcagatgg cagctcgcca
    14581 aaagacttga tgacttcatt ttctttgtaa ccatccagtt cgactacttg tttattcact
    14641 ggttgagcac caccaggaga catcaagacc cacttttcca tgtttttcgg ttgagataag
    14701 aaagctgtga atgcttcagc agcctctttt tgtttctcgt ctagaccaga agaaatcgtc
    14761 aatccagata ctgtgccata gacagcttga tctttttgtg ttgggatagc aaaaccgata
    14821 ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt aagttgaata gattgccatt
    14881 ggtgctgttc cattcatgaa agcatctttg atttctgtca catcattaga gcctggcatc
    14941 gtgtattggg ataagttttg ataaaacgac aatgcttctc tcatcttttc cgtatcgatc
    15001 gttacctcgc cttcatcatc caaaacattt gctccatttg acaaagcaaa ttgagagaac
    15061 gcttgctcac tcattgttcc ttcagcagta ggcatagcaa ttccatattg tttattctct
    15121 ttatctgtga aatgttgagc aatttttaaa atatcatccc aattttctgg ttctgaaaaa
    15181 ccagcttctg ataatttttc tttgtcatac caaatacctt gaacccagcc gctaattggt
    15241 gcagcaatat agctttttcc atcttctgaa cgaacaaggt ttttggctcc gtcataataa
    15301 ttatcttctc cagcttcctc gataacgttt tggactgccg tctgatcaat cagttcatct
    15361 ttatccatca ctttagcaaa atcctgactg acttccatta cttctggtaa tttaccggaa
    15421 cgagctaacg taacgatttt tgtattgaac gctgattctt ctaccggcac ttgcttgatt
    15481 ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt cattgatcac acttaatcgt
    15541 tcctgttcga cagaagagtg catgaattga atcgttgtct gttgtgattc gttagagctt
    15601 tgactttcac cacaagcagt aagaagaaat ccagtagcta gcagacaggt agtaacaata
    15661 atttttttca tttttttcca cctcttattt tattattttc agccacatat attcatacgg
    15721 ctggaatatc aactttcgtt cttgactgca atggataatt tgtccagaaa acaggtctag
    15781 gtaatctcct tttggtaaaa gataacctgc tgattgattg gaaaaattat ggataactaa
    15841 gactttctct tccttcgctt cgcgaataaa actgataaaa gaatctgctg agtccagtaa
    15901 gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt cgaatagata gcatctgggt
    15961 caattcacca tatactgctg ttcttaatgg atctgtctgc aactctttct caatatctgc
    16021 taacaaaaat ttttgccgat tgatttttcg attctctcct gttttttcta cgcctttgta
    16081 atcatttcga cttcccagca cactttgaat ataaattgcc ggcacgcctg gtaacgttac
    16141 caaaaatgaa tgagcagcta aaaagcgttt gcttctccat tccgtcgtat ctgttggaga
    16201 acttaacgca tctagatacg tagcgtttat ctcatatgga ctggtcgttc catctgggtt
    16261 tttcttgtag tttactcggg caccctcttt cttcagctgt tccactaatt caaggatcgt
    16321 tttttccggc agaatacctc gtaccggatt caatccgata ccgtcatggg aggctaaaaa
    16381 attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt tttaaccatt ctttcaactt
    16441 acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt ggtagcgaaa actgatagac
    16501 catctgtgct tcattctcgc cattcccaaa ataactgata ttatctttgt gtggcacatt
    16561 tgtctcagtg atcaataccg ttcctggagc tgcttcatca gcgattgcac ggaataattg
    16621 gatgatttta tgcgtttctg gcaggtgtat acaagaagtt cctagttctt tccataaaaa
    16681 tcccacagcg tccaagcgaa taaatgaagc gcctttttct aagtaaaaca aaagaatatc
    16741 gatcattttc aataaaactt ctggattttt aaaattcaag tctacttgat cttcgctaaa
    16801 agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg aacgttgtaa gtaacgggct
    16861 ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt ggcaaatcaa taaaaaaatc
    16921 tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa gtactttcgg cagacatatg
    16981 attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc atttcttcta tatcttccca
    17041 tgtgcccaaa cggtcgttga ttgcctgata atttacaacg gagaaaccat catcagatgt
    17101 atatgggaaa aatggcaaga aatgtacaac tctaaatgta tctgccaaat acttttgata
    17161 aaatttctta aatgtacgca aagttggttg ctgctcttca aaaaattgat ccccataagt
    17221 aatcaagaaa atatcttctt cgtcccaatg cagtttgcct gcctggcctt tcttctgctg
    17281 ataaacactg actttttgtt caatcataga ggcggttttc tcagacaaat ctgagtcgcg
    17341 ataaatcgct tgtaaaagtt ttgtatacat cggattcccc tttctttttt gggagcgttc
    17401 ccaacgattt gatcatatta tggaaaacgc ttactgtcaa tagcattttt ttaaataatt
    17461 ttgatataca caaaaataca aataagaaag cttatgtatc aataattaat agcttcttaa
    17521 aaaaatatgg tctctcatga tcatgcaagc tattcctaaa ttttcaaaat catagacaaa
    17581 aaagaatgtc tattgtacta tggaattatt ctaaaacgct cccaaaaagg aggtgcatat
    17641 aaaattgaaa cctactattt atgacattgc gaaaaaagct aatgtctcaa aatcaactgt
    17701 ctccagagta ttaaataacc agccaaatat ctctgataaa gcaaaagcaa gtgtacttca
    17761 agcaatcaaa gaattgaatt atgaaccgag caaaattgca agaggactgt caggaaatgg
    17821 ttttgacgcg attctagcat tatctcatcg tacttctcat acgacctatg gcaatccttt
    17881 tttttcagat gttattcaat ccatttctgc cgtttctgaa caaaataact tcgacttgat
    17941 tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa tgcgcgaaaa agatccaaga
    18001 aaaaatgatc cgaggaatta ttattttaag ctctacgatg gatgaaacac ttctttttga
    18061 attggatcaa tacaatattc ctattgtggt aatcggaaaa gtcggaaacg actacacgaa
    18121 catctactca gtggacacgg ataatttcca ggatagttac gatttggtgg aaacactgat
    18181 caagaatgga caccgtcata tcggctgtct ccatgctccg acagaagttt atgtatccaa
    18241 tgatcgtctc aatggttacc gtcaatgctt atttgatcac cacctagata tccgtaatga
    18301 atggattata gacggcggct ttcgcttaga agatagcatg gcagcaatga atgatttaat
    18361 gaagctacga gaaagaccaa ctgcgatttt ttctaccgat gcattaaaat cattgagtat
    18421 ttacaaagtg gcagaacaat ctggaaagca gatacctgat gatctttcga tcgcaggatt
    18481 cagtgatccc ttattgtcct ccctattttc gccacagttg actcggatag acattccgac
    18541 aaatcagtta ggaatcaaag ccacagaact tttattccaa ctaatccacc accaataccc
    18601 tgaagaaaaa agaatcattc tttcaaccga gattactctc acagattcga tccaacaaca
    18661 agaaatgtag aacagaatta taaaagaaca gaaacactga ggataagtca gttactctgt
    18721 tcttttttca atcactcatc cctcgttacc ccctcccggt attttagcga atttttctta
    18781 ttttccatct gttttagata gctgtggata actataatgt ttgtgttaaa ctattttttc
    18841 cacagggaag aaaaaactat ttttaaccac gttgtgtata agtttttata gttcgttttt
    18901 ttaaacacat ttacccttat ttcaatccat gttatttggg gatacttatc tctaaaacat
    18961 aaatagaata acagacatgt ctactggttt tgttttattc gaggaattat tcaataatag
    19021 ctagcaaaat attaaaaata accgctctat cttcccatca atttattttg cattcaaaaa
    19081 atgatctatc tttacttttg ttgattgact tctcttggaa gatgatcgtt ccgatacctt
    19141 aattggatac gaaccgatgt tgttaagatc ctttcccact tttcttctag ttagacaaat
    19201 tattttagac aaaccaaaaa agaggttgtg acaataattt gtcacaacct cttttttaaa
    19261 atatctattt tgtttgattg ttcattgcgt cttctgcagc catttgcgct tcaatatctg
    19321 aaatactgta gacattttcg cttgctacac cgacttcttt tggttccatg tcacgataac
    19381 gttgcatacc agtaccagct gggatgatct tacctataat aacattttct ttcaaaccaa
    19441 gaagaggatc tttcttaccg cggatcgcag catccgtcaa gacacgagtt gtttcttgga
    19501 atgatgcagc agataagaaa ctatttgttt ctaatgatgc ttttgtgata cctagtaaga
    19561 caggtcgaga agttgcagga acgcctcctg agactagcgt attgtagttt tgatctttaa
    19621 agtctgcgat gtctaataat gtacctggta agatatctgt atcacctgga tccattacac
    19681 gaactttacg cagcatttga cgaaccatta cttcgatatg cttgtcgccg atttctaccc
    19741 cttgcatacg gtaaacacgt tgtacttcac gaagcaagta gttttcaaca gataatacat
    19801 cgcgaacttg caacaattgt tttggatcga ttgatccttc tgtcaatggt gcaccacggt
    19861 ggatcatatc gccttctgct actttcatac gtgctgtata aggaacagta tacgtacgtg
    19921 tatctgtttt tcctttgatt gttacttctt tggtacgtgt tgcaggatct tctgagatat
    19981 cgatgacatc ccctgttact tctgtgatca ctgcttgccc tttcggatta cgtgcttcaa
    20041 agatttcttg gacacgagga agaccttgag tgatatcgtc tccggcaacc ccacctgtat
    20101 ggaatgtacg catcgtcaac tgagtaccag gttcaccgat tgattgagcg gcgatcgtac
    20161 caactgcttc tccgacttca acgtcagagc cagttgcaag gttgcgtccg taacaatgtt
    20221 tgcagacacc atgttttgta ttacatgtga agacagaacg gattgtcact gtttcgacac
    20281 ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct gcaatgatca
    20341 attcatttgt ttcaggatga cgtacttctt tacgggtgta acgtcccact aaacgatctt
    20401 ctaatgattc gatgatttca tttccctcgc ggatggcttg gatttcaaga cctcggtcag
    20461 taccgcaatc ttcttcacgg atgattacat cttgggccac gtcaaccaga cgacgagtca
    20521 agtaacctga atcggcagtc ttcaaggctg tatcggtcat ccctttacga gccccgtgag
    20581 ttgagataaa catttccaag acggatagtc cttcacggaa gttcgaaatg attggcaatt
    20641 ccatgattcg accattagga gcggccatca aaccacgcat tccggcaagc tgtgtaaagt
    20701 tggaaatgtt accacgagct ccagaatcag acatcatgaa gattggattt ttcgcttcca
    20761 aaccttccat cagtttttgt tggatgctgt cttttgcagc attccaaaca gcaatgacac
    20821 gttcataacg ttcgtcatct gtgatcagac cacgacggaa ttgttttgta atagtttcaa
    20881 cttgtttatg ggcattttca atgatttctt gtttttcatt cagtacactg atgtcagcga
    20941 tccctactgt gataccggca tgtgtcgaat gtttgtaccc taagtctttc atacggtcaa
    21001 gcatcttaga agtttctttt actttgaatc gtttgaagac ttctgcgatg atgtttccta
    21061 agtttttctt cttgaacggt aatactaatt cttgctcttt gatatgtgca ggaatatcag
    21121 taccagcttc aacaaagtat ttgtccggtg tttggacggt caagttgaag tctgttggtt
    21181 cattcagata agggaattca ggaggcatga tctcgttgaa gatgatctta ccaactgttg
    21241 tgatcatcgt acgttctttt tgccattcag taaatggttt ttcacctaat gagttagggt
    21301 tcacaccgat acgtgaatgt aaatgaacat atccgttgcg ccatgcgata acggcttcgt
    21361 tttgatctct gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt gtcaagtagt
    21421 agttacctag aaccatatct tgagaaggag taacaactgg tttaccatct ttagggttca
    21481 agatgttttg agcagctaac atcaacatac gtgcttctgc ttgtgcttct tcattcaatg
    21541 gaacgtggac agccatttgg tctccatcga aatcggcatt ataagcttca catacaagtg
    21601 gatgcaaacg gattgcgcgt ccttgtacta aaactggttc gaatgcttgg ataccaagtc
    21661 ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc cttgattacg tcttctaaaa
    21721 catcccatac ttcgtcttct tgacgttcga ttttacgttt cgcatttttg atgtttgaag
    21781 ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt gaataattca atggccattt
    21841 cttttggaag tccacattgg tacatcttca agaaaggtcc aacaacgata acggaacgac
    21901 ctgagtagtc gacacgttta cctagcaagt tttgacggaa acgaccttgt ttccctttca
    21961 acatatgaga aagagatttc aatgggcggt tacctggtcc agtaactgga cgtccgcgac
    22021 gaccgttatc gatcaatgca tctactgctt cttgcagcat acgtttttca ttttgaacga
    22081 tgattccagg tgcattcaag tctaacaaac gtttcaaacg gttattacgg ttgattacac
    22141 gacggtaaag atcattcaag tcacttgttg caaaacgtcc accttctaat tgaaccattg
    22201 ggcgtaaatc tggtggaata acagggataa cgtccataac catccagctt ggttgatttc
    22261 ctgaagcgcg gaatgcttct aaaatatcca aacggcggat tgcacgtgta cgtttttgtc
    22321 cttgtgcaga tttcaattct tctttcaatt cggctgcttc tgcatctaga tcaacattat
    22381 caagtaattg tttaatcgct tctgcaccca tagcagcttg gaattcttgt ccatattgtt
    22441 cacgtttttc gcgatattcg cgttctgtca acaattgttt tttctctaaa ttcgtatttc
    22501 ctggttcgat gactacatat gaagcaaaat aaatgatttc ttccaaagcg cgtgggctca
    22561 tgtctaatac aagacccata cgtgaaggaa tccctttaaa gtaccagata tgtgaaacag
    22621 gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga gtaacttcca
    22681 ctccgcatcg atcacaaacg attcctttat aacggatacg tttgtatttt ccgcaggcac
    22741 attcccagtc ttttgtagga ccaaaaatcc gctcacagaa caacccttcg cgttcaggtt
    22801 ttaaggtacg atagttaatc gtctcaggtt tttttacttc accgtaagac cagcttctga
    22861 ttttttcggg agaagccaaa cctatttgca tactttcgaa tttatttaca tcgatcaaaa
    22921 ggggttccct ccatttcatt taatggcaaa ccgcaagacg gctgatcttc gaccgccttg
    22981 cagctcatag accggattag tctaaattat cttctgcggt ttcaaagttt tggatgacat
    23041 cgtctttttc atcttccact tgttcagcag ctttcttttc taattctttg gcagtttgct
    23101 gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca tcgtccatgt
    23161 cgcgaagttc gatctctgtt tcttcgatat ccaagacacg catgtccaga cctagtgatt
    23221 gcaattcttt caccaatacg cggaatgatt ccggtacacc tggttttgga attggttcac
    23281 ccttgacgat tgcttcgtat gttttcacac gtccgactac gtcatctgat ttgtatgtca
    23341 agatttcttg taaagtataa gccgcaccgt aagcttccag tgcccatact tccatttcac
    23401 caaaacgttg tccaccaaat tgagctttac ctcccaatgg ttgttgtgta accaatgagt
    23461 aaggtccgat tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt ttgatcatgt
    23521 acatcacacc gacagacaca cgtccatcaa atggttcacc tgtacgtccg tcgtacagaa
    23581 cagttttcgc atcgctggcc ataccggctt cacgaactgt ttcccatacg tcttcatcac
    23641 ttgcaccatc aaatactggc gttgctacgt ggatacctaa ctgacgtgca gccataccca
    23701 agtgtaattc caatacttgc ccgatgttca tacgtgaagg cacccctaaa ggattcagca
    23761 tgatatcgat tggtgttcca tcaggtagga atggcatatc ttcttccggc ataatacggg
    23821 atacaacccc tttatttccg tgacgaccgg ccattttatc cccttcatgg attttacgtt
    23881 tttgaacgat ataaacacga actaacatgt ttacgcctgg tgacaattca tctccagctt
    23941 cacgagtaaa gattttcaca tcgtgaacga taccgccgcc gccatgtggt acacgtaatg
    24001 atgtatcgcg gacttcacga gctttttcgc caaagatcgc atgcaataga cgttcttctg
    24061 cagataattc tgttacccct tttggcgtga ctttccctac aagcaaatcg ccatcttgga
    24121 cttctgcacc aatacggatg atacccattt cgtctaaatc ttttaatgcg tcttccccga
    24181 cattagggat ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg gcttctgatt
    24241 catattcttc aatatggata gaagtgtata cgtcatcttt taccaaacga cggctcatga
    24301 tgatcgcatc ttcatagttg tacccttccc aagtcatgaa accaactaaa acgttttggc
    24361 ctagagccat ttcgccttgt tccatagatg gtccgtctgc cagagtatcc cctttttcga
    24421 ctttctcacc caaatgaacg attggacgtt ggttgtagct tgttccggag tttgaacgac
    24481 ggaattttgt tacatcgtat ttatctaatg caccgttgtc gcggcgtacg cggatttcag
    24541 aagcatcaac gtattcaacg acaccatcat gtttacataa caaagcagct cctgagtcat
    24601 gcgctgattt gtattccata ccagtaccaa cccatggtgc ttgaggattg atcaacggca
    24661 ctgcttgacg ctgcatgttg gcacccatca aggcacggtt ggagtcatcg ttttccaaga
    24721 aaggaataca tgctgtcgca acggcaacta cttgtttagg tgatacgtcc atgtagtcta
    24781 ctttatcaat agatacttct aagttttcac tttgagcacg agccataaca acgtcttgag
    24841 caaatgttcc atcttcattc aatgggctgt ttgcttgggc tacgatgtaa tggtcttcga
    24901 tatcagctgt caagtagtcg atttggtcag taacacgacc tgtttcacgg tctacacgac
    24961 gataaggtgt ttcgataaat ccgaatttat ttactttcgc ataactggac aaactattga
    25021 tcaacccgat gtttgggcct tcaggagttt cgatcggaca catacggcca tagtgggagt
    25081 agtgaacgtc acgaacttca taaccggcgc ggtcacgagt caatccgcca ggtcctaagg
    25141 cgaaaagacg acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc atgaactgtg
    25201 acaactgaga agaaccaaag aattctttga tacttgccac aactggacgg atattgatca
    25261 attgttgagg tgtcaatgtt tcagtatctt ggatcgacat tctttcacga accacacgtt
    25321 ccatacgtgc tagcccgata cggaattggt tttgtaaaag ttcaccgact gaacggatac
    25381 gacgatttcc taagtggtcg atatcatcga cattgccgat accttccatc aagttcaaga
    25441 aatagctcat tgaagcaatg atgtcggctg gacgaaccgt ttttacagct gcttctggat
    25501 agccgtttcc gatcacgttt acttcacgtt cagggtctct tggtgaaaat actttgatga
    25561 cttggacagt cattggatct gtgaccacac cgtcttctga tggatagtaa gtcactgaat
    25621 ttaggccatt atcaatgaaa ggagctagtg tttccatgac ttggtgagtc agaactgttc
    25681 ctttttcaac gatgatctcg cctgtttctg gatcaactaa tgtttcagct aatgtcagat
    25741 tcaatagacg tgttttcaaa tctaattttt tgtttacttt gtaacgacca acgtttgcta
    25801 agtcgtaacg ttttggatcg aagaaacgag cattcagcaa gttacgtgag ctgtctgctg
    25861 tttttggttc gcctggacgc aaacgttcgt aaacgtcttt caaaccttct tctgttcttg
    25921 agtcgcttgc gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt tcgctgtcgc
    25981 cgaagatttc aaagatcgta tcatcggaac caaatcctaa cgcacgaact aatacggtta
    26041 aaggaatctt acgtgtacgg tcgatacgta cataagagat gtcttttgca tcagtttcca
    26101 tttctagcca agctccacgg ttagggataa cagtagaacc gaatccttct tttccgtttt
    26161 tgtcgacttt tccatggaaa tagacacctg gtgaacggac taactgagaa acgattactc
    26221 gctctgcccc gttgatgatg aatgttcctt gttctgtcat taatgggaaa tcaccgaaga
    26281 atacttcttg tgctttgatt tcgccagtct cgcggtttgt caaacgcaat gttacatgta
    26341 atggtgcaga gtagtttgca tcatgcgcac gagcttctgc tactgtatac tttggttctt
    26401 tcaattcata atctacaaat tccaaagata gattgccatt gaagtcatcg attggcaaaa
    26461 tgtcttcaaa catttctcta agtccctcat ctaagaacca ttggtaagag tctgtttgaa
    26521 tttcgatcaa atttggtaat tccaacactt cactgattct tgcgaaactt ctacgttcgc
    26581 gatgttttcc gtattttact acgtgtccag ccaagctctt cacccctcta aaatgtcatt
    26641 caaaaagact acccgatcaa ttgttacaat aatatttcaa atattatttt taggtaacaa
    26701 cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt ctcttgaaac
    26761 cttacttcgt gagcttctac ttttgtttcc ttatattaaa gccgaaacgg acaatatttc
    26821 gtttctgcct tttcttcatc agatagtttt tgcaacttca tatactacac ttattttcta
    26881 taaaagtcaa cacttcctgg cgtttcttct gctttttcgc aacattttct taatgttttc
    26941 aattttatgt tttcaattat ctgacaattc ttaattgaca gaccttttta tctcggttat
    27001 actttagaaa attcattttg tggaggaact tctatgcgaa ccaacatcaa ccaaatcgca
    27061 ccaacacaac tgaataactt ttactttagc tactttagat aggtttgtat tcatcatgat
    27121 ggatacaaac gttaaacgac agtttgtatc tatgatggct aaagaacttt gtattgcagc
    27181 aaaaagccac gtagacaaaa tgctaagtgg ctgagcagtg aaaatacaaa gtaaaagtat
    27241 caagcattct actttttcaa tttattttcc gacttttcaa tccatttgga cattttgtct
    27301 gaatggattt tttgtttccc aaaattaagg aggaaaaaga aatgaaaaaa aatataatca
    27361 tcggattagg cgctgccctg ctactagctt taggaggttg cgcacaaaaa gacaattccg
    27421 cagaggcatc agatggacaa aaaacaattg gcgtattgca acttgtacaa cataactcgt
    27481 tagatgctgc ctataaagga ttcaaagaag gattagcaga agaagggtat aaagaaggcg
    27541 ataatctaac aatcgattat caaaatgccc aaaacaatca agacaacctg aaaagtatga
    27601 gtgagaaact cgtcaaagaa gatcctgatt tgctacttgg aatcgccact ccagcagcac
    27661 aatctttatt aaatgaaaca accactatcc cgatgactgt cacagcggtc acagacttgg
    27721 aacaagccca tttagtcaca tccaatgaaa aacctggcgg caatgtgact ggcacgacag
    27781 atatggtgcc aatcgaaaaa caaatcggct tactgttaaa tatcgtgcca gacgccaaaa
    27841 caatcggcat catgtataac aacggtgaag caaactctaa gatccaagct gatttggcag
    27901 aaaaagaatt gaaaaaagcg ggcgtcaaag tcaaagtact gacagcaaat tccacaaatg
    27961 atgtccaaca agtgacgaaa agtctagcta aagatgtaga tggaatctat attcctactg
    28021 ataacacgtt cgcaagtgcg gcaagtgttg tcggtgaagt cgcaaaagaa acaaaaacac
    28081 ctgtcgttgc tggcgcagtt gatcaagtca aagacggcgg tctagcaaca gtaggaatcg
    28141 actacgaaag cttgggcaaa caaacaggga tcatggcagc aaaaatctta gacggcaaga
    28201 atccaagtga acttcctgtc gaaacagcga atgatttgga actgtatgtc aataaagaca
    28261 tggccaaagc attaggcatc aatccagaca gcatcaaaga agctgaataa aacacaggag
    28321 gattctcatg acaaatttgc tctctctttt cttatcatct gcatcgcaag gggtcttatg
    28381 ggccctactt gcgattggtg tatacgtcac tttccgtatc ttggacattg ccgacttgac
    28441 tgctgaagga agctttcctt taggtgcagg gatcgcggca gtaagtatta caaatggcta
    28501 tcatccattg acagcttgtc ttctaggctt tttagggagt gcggcagcag ggcttgtttc
    28561 cggactgctt cacacaaaat tgaaaattcc cgcccttctt gcagggatca tcaccatgac
    28621 tggtttatat tctgtcacat cccgtgtgat gggtgcgccg aacatctctt tattgggtca
    28681 gccaacgatc tttcattggg tcgagtcatt cggcataaca aaagacaacg ctgtactgat
    28741 cgtcggtctg ctttttgctt tgatcgttgt atgtttactt gttttgttct tacgtacaga
    28801 aacaggactt gctattcgtg caactggcga taatctggca atgagcgaag caaacgggat
    28861 caacacagat aatatgaaga tcatcggtta tatgatttct aatggatgca ttgctctttc
    28921 tggctccttg cttgcgcaaa acaacggctt tgctgattta aattctggta ttggaacgat
    28981 cgtaattgga cttgcttcta ttatcatcgc agaagttttg ttacgtaacc agccactatt
    29041 tctccgtctc ttgactgtcg ttttaggtgc ggtcatttat cgttttattt tagcgttagt
    29101 cttcgaactg aatgttgaac caagtgattc gaaacttgct tctgctttcg tgctagtgat
    29161 ctgtctttct ttcccgcaga tccagcaaaa attccattta cctcaaaaaa agaaaggcgg
    29221 tagtgtcaaa tgaatgacgt cttaacattg gaaaatctcc atcatacctt tgaaaaagga
    29281 acgatcaacg aaaatcatgt cctgaaaggc attaatcttt ccttagatcc cggcgacttc
    29341 gtgacgatca tcggtggaaa tggggctggt aaatctactt tgctgaacag catcgccggt
    29401 acattttctg tcgatcaggg tcaaatttta ttgaatggaa aaaatatcac gaaaaaatct
    29461 gttgtcgaac gttcgaaatc catcagtcgc gtattccaag atcctaaact agggacagcc
    29521 gttcgtttaa cggtagaaga aaatcttgct cttgctatga aacgaggaaa aaaacgcggc
    29581 ttcttccgtg gtgtcaaacc acaagaccga acatttttca aagaccagct ggttcggcta
    29641 aatttggggt tggaaaatcg tttatcctca gaaatcggtt tgctgtctgg aggacaacgt
    29701 caagctatta cgttattgat ggccacatta cagcgtccag aactgatctt attagatgaa
    29761 cacactgcag cgcttgaccc tgctacttcc aaaacggtga tggaattgac agaaaagctg
    29821 atcactgagc agaatctcac tgcttttatg gtcactcatg acatggaaga cgcgatccgc
    29881 tatgggagtc gactgatcat gctgcatcaa ggaaggatcg tcgtagatat ccagcaagag
    29941 gaaaaacagc agctcacagt gccccaatta ttagcgttgt tcaaacaaaa cagcggcacg
    30001 gcattaaaag acgatcaagt acttttatct tcataatcag aaagaggatg ggacaaaaaa
    30061 tgaacgccca ccctcttctt ttttctcttt ccttctttcg tttttgcttt atgtatcaac
    30121 cgttgtttct tcgaaaacta ttttgttctc tccttcttcc tgatagaatg gatgggaaag
    30181 gagcttgatg ccatgacaac gaaagacaag gtacttcaat tattgaaaaa gacaccagat
    30241 tttctttccg gagaaaagtt ggcacagcaa ctggaagttt cccggacaag tatttggaaa
    30301 gccatcaaag aattggaaaa ggcaggctat cgttttgagc acgggcctac agggtatcgt
    30361 tacctgccct ctgacgtact agatgcaaaa gaaatccagc aagagctacg gtcgtttatt
    30421 cctgatttga aagtacaaac gatgatgact tctgaatcta cgatgaaaga tgccaaactg
    30481 gcagcagctc aaggagattc tgtttcgaag ttatttgttg ctgatacaca agaacaagct
    30541 cgtggacgtt ttggacggcc ttttttcgct gaacctggac gaggaatcta catgagtctc
    30601 ttgctccatc cagacaaacg gtttgatgaa ttgcctcaat atacgatcat cgcagccagt
    30661 gcctgtgtga aagccattga atctgtaacc ggtcgttctg cttcgatcaa atgggtcaat
    30721 gatatctaca tggatggaaa aaaagtctgt gggattttat ctgaagcagt cagtgatatg
    30781 gaaagcggca ggatcagtca tatcatcatt gggatcgggc ttaatttttc tgttcctcaa
    30841 aatcattttc cagctgatct ccaagaaaaa gccatctctg tctttcccga cggacaagct
    30901 agtgtcacaa ggaatcagct aatcagcaaa atctggcaag aattgttcac ttatctttct
    30961 cacccagaaa aagcgttgga cttctataaa gaaaaatcat tcgtattggg aaaaaccgtg
    31021 tcgttcaatc aaaaaggaac gacttatgtc ggtcttgcaa aagaaatcgg tgatcatggc
    31081 gaactgatcg tccaaacaaa tgaacagaag atgagtttgt cttctggcga aatcagtctc
    31141 acatctatcc gtacgcaata attatcagat ttcacaaaag gacacctgat ctcatacagt
    31201 gtcctttttt ctgaattctc ttcaaaagac tctccttatt ctatcagctg acagtttctc
    31261 tatcctctag aaaaaggtat tctaaaaata tctataaagc atggtacact ttctttaaat
    31321 gaatcaatgc aaaaaacaag agagtcatca gtttacacga tcaaaagaga tagcaggccg
    31381 cttccttttt caatcttata gatagaccat gggtaacgat aaaaacaaaa aagaacttgc
    31441 gacttatttt gccgtaagtt cttttttagc tagtgcctta atctcttaat cccaacaaac
    31501 gtctcgcata gatcacctga ccagagtgca tagcagcatc atcaataata gaaacgagac
    31561 gcattcctcg tgtgactggt ggtgtccagt tctcgtcaat tacttcatct agctcggaag
    31621 gttccagcct tcttaagtaa gaaatagcaa aatcagcagc tcctcgcaag tatcctagca
    31681 gatcttctat ttgattagct tggatctgct ttgcttgttc cagactatgc ttccaatctt
    31741 gttcgttatc tgctatcgtc atcggaaacc ggctttccca gtcttgacta tgccaaattg
    31801 gagaagttcc tgctaaatca gcaatctgga aatctaattc tcgagcggta tgccaagcca
    31861 gccaagtcat tgatttgatc tgcggggctc tatcttccac tggaaaacga ttggcctcct
    31921 caatagagac gccttcaaac aaacgttcaa accgatcgta tgcccgatcc acactctcgc
    31981 atagcatatc taaataatcc atttattttc ctccttttta tttatcctac ttactttttc
    32041 ctaattctag gatacctact tatatgttac tacacaccct tattttttac actttaacag
    32101 tcaccatcat caatagtttg gagattgaaa gaaaaagata gtgtcgaact atcaattgaa
    32161 caagaagcaa caatatgaac gtttcattaa tattctttaa ataaacaatg atttttcaaa
    32221 cgaacagttt aagactagga gaaaaagagt ataataggaa aatgtttgaa aggaaaggaa
    32281 ggggaaaaat gaaaaaaagg gcaagtttac tagtcctagt tgctttgttg ttcaatatct
    32341 tttcgtatgg tgtggccgtt tcagcggaaa catacattcc taaagaccct gggaatattt
    32401 ataatgatct aactggtaac tatggcttgt taggaattgc tagtcaattc cacgtatttg
    32461 ctaaaaataa gacaactgtg aatgcacata cagacggaaa tatagcaaca catgaacttg
    32521 atgcaaaaaa taattttggt acagagcttc atgaaggttt attgaaccaa gaaataaatt
    32581 atatacaaaa aactgattct ttcgctaatt cttcaggcat acctacaggt agcacaagaa
    32641 tgaataaatt tgtcgttggc agtaacgtgg atgtagcact tcaagatggt catcctacag
    32701 tcaatggatc caggcttgat cacttaactc cagatgattt ttataaagat cgtgctgggc
    32761 atgaatatat tgatttcgag caggaatttg ctaaattgaa tgtagcagca aatgatcttg
    32821 caacgatcac tccagctaaa acatatactg ctgcagattt tccagacatg aacaatcgga
    32881 caatcgattt aactgattta tcagataccg ggtttttatt ggtaaatatc gatgcagaag
    32941 tgttaacgat gaatacgcca cttcaaatca ttaatccaaa tgatcaagta gttgtattta
    33001 atgtcatcaa cagttcttct gcattgaacg tacagtcacc aatcaaatac aatgatcgtt
    33061 caaatcatga aacagaagat ttttcggatg cgaatatctc ttggaatttt ggaaatgaga
    33121 tgactgattt gacaatcagt gctccttttc aaggaacaat ccttgcgcct aatgctacta
    33181 tccgcattac tcaaaaccaa gacggaacga ttataggaac aaatgtgatt ttagatgcag
    33241 ctaccaatcg ttgggatcct aacgaaatat ttatcagcga caatggtaca gatacgacag
    33301 acacaagcga cagtacagat acatctacca caagtgatag ctcagattca tca
//
LOCUS       DASJST010000014        33263 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJST010000014 DASJST010000000
VERSION     DASJST010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.014"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..229)
                     /locus_tag="U4B05_000491"
     CDS             complement(41..229)
                     /locus_tag="U4B05_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN2049653.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="U4B05_000492"
     CDS             499..861
                     /locus_tag="U4B05_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2049654.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="U4B05_000493"
     CDS             913..2595
                     /locus_tag="U4B05_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN2049655.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDDIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="U4B05_000494"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="U4B05_000494"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN2049656.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="U4B05_000495"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="U4B05_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN2049657.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="U4B05_000496"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="U4B05_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2049658.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="U4B05_000497"
     CDS             6430..7434
                     /locus_tag="U4B05_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049659.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="U4B05_000498"
     CDS             7435..8379
                     /locus_tag="U4B05_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049660.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /locus_tag="U4B05_000499"
     CDS             8379..9341
                     /locus_tag="U4B05_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049661.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="U4B05_000500"
     CDS             9368..10282
                     /locus_tag="U4B05_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049662.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="U4B05_000501"
     CDS             10308..12089
                     /locus_tag="U4B05_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2049663.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /locus_tag="U4B05_000502"
     CDS             12437..13123
                     /locus_tag="U4B05_000502"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN2049664.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="U4B05_000503"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="U4B05_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN2049665.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="U4B05_000504"
     CDS             16733..17542
                     /locus_tag="U4B05_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2049666.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="U4B05_000505"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="U4B05_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN2049667.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="U4B05_000506"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="U4B05_000506"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN2049668.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="U4B05_000507"
     CDS             complement(21094..21546)
                     /locus_tag="U4B05_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN2049669.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="U4B05_000508"
     CDS             21696..22646
                     /locus_tag="U4B05_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2049670.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="U4B05_000509"
     CDS             22639..23598
                     /locus_tag="U4B05_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2049671.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="U4B05_000510"
     CDS             23595..24350
                     /locus_tag="U4B05_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2049672.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="U4B05_000511"
     CDS             24373..25326
                     /locus_tag="U4B05_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2049673.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="U4B05_000512"
     CDS             complement(25970..26266)
                     /locus_tag="U4B05_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN2049674.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="U4B05_000513"
     CDS             26621..26977
                     /locus_tag="U4B05_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2049675.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="U4B05_000514"
     CDS             26987..27886
                     /locus_tag="U4B05_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN2049676.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28403..29134
                     /locus_tag="U4B05_000515"
     CDS             28403..29134
                     /locus_tag="U4B05_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2049677.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="U4B05_000516"
     CDS             29279..31306
                     /locus_tag="U4B05_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2049678.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="U4B05_000517"
     CDS             31414..32511
                     /locus_tag="U4B05_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2049679.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32818..>33263
                     /locus_tag="U4B05_000518"
     CDS             32818..>33263
                     /locus_tag="U4B05_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2049680.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11139 a         5762 c         7122 g         9240 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tgatatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa
    32581 tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt
    32641 gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg
    32701 tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat
    32761 ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg
    32821 aattatgagt tttttttaag caaagaagat cacagaagtt attcattact taaatattta
    32881 gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg
    32941 acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta
    33001 gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa
    33061 tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta
    33121 ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg
    33181 agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa
    33241 attttttttg aaaagagaaa gtt
//
LOCUS       DASJST010000015        32778 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJST010000015 DASJST010000000
VERSION     DASJST010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.015"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..874
                     /locus_tag="U4B05_000519"
     CDS             131..874
                     /locus_tag="U4B05_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049681.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            880..1245
                     /locus_tag="U4B05_000520"
     CDS             880..1245
                     /locus_tag="U4B05_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049682.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            1261..1791
                     /locus_tag="U4B05_000521"
     CDS             1261..1791
                     /locus_tag="U4B05_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049683.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            1953..2261
                     /locus_tag="U4B05_000522"
     CDS             1953..2261
                     /locus_tag="U4B05_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049684.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            2257..2676
                     /locus_tag="U4B05_000523"
                     /pseudo
     CDS             2257..2676
                     /locus_tag="U4B05_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2813..3613
                     /locus_tag="U4B05_000524"
     CDS             2813..3613
                     /locus_tag="U4B05_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049685.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            3650..3832
                     /locus_tag="U4B05_000525"
     CDS             3650..3832
                     /locus_tag="U4B05_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049686.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            3943..4260
                     /locus_tag="U4B05_000526"
     CDS             3943..4260
                     /locus_tag="U4B05_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049687.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            4263..4495
                     /locus_tag="U4B05_000527"
                     /pseudo
     CDS             4263..4495
                     /locus_tag="U4B05_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4610..5089)
                     /locus_tag="U4B05_000528"
     CDS             complement(4610..5089)
                     /locus_tag="U4B05_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049688.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            5209..6546
                     /locus_tag="U4B05_000529"
     CDS             5209..6546
                     /locus_tag="U4B05_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2049689.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            6569..7096
                     /locus_tag="U4B05_000530"
     CDS             6569..7096
                     /locus_tag="U4B05_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049690.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            7083..7646
                     /locus_tag="U4B05_000531"
     CDS             7083..7646
                     /locus_tag="U4B05_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049691.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            8068..8805
                     /locus_tag="U4B05_000532"
     CDS             8068..8805
                     /locus_tag="U4B05_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049692.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8822..9238)
                     /locus_tag="U4B05_000533"
     CDS             complement(8822..9238)
                     /locus_tag="U4B05_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049693.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            9631..10878
                     /locus_tag="U4B05_000534"
     CDS             9631..10878
                     /locus_tag="U4B05_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2049694.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            11146..13536
                     /locus_tag="U4B05_000535"
     CDS             11146..13536
                     /locus_tag="U4B05_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HEN2049695.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            13585..15420
                     /locus_tag="U4B05_000536"
     CDS             13585..15420
                     /locus_tag="U4B05_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2049696.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            15489..16352
                     /locus_tag="U4B05_000537"
     CDS             15489..16352
                     /locus_tag="U4B05_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2049697.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            16342..17451
                     /locus_tag="U4B05_000538"
     CDS             16342..17451
                     /locus_tag="U4B05_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2049698.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            17790..18590
                     /locus_tag="U4B05_000539"
     CDS             17790..18590
                     /locus_tag="U4B05_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049699.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18678..19484
                     /locus_tag="U4B05_000540"
     CDS             18678..19484
                     /locus_tag="U4B05_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049700.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            19716..20585
                     /locus_tag="U4B05_000541"
     CDS             19716..20585
                     /locus_tag="U4B05_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN2049701.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            20605..20952
                     /locus_tag="U4B05_000542"
     CDS             20605..20952
                     /locus_tag="U4B05_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049702.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            20965..22227
                     /locus_tag="U4B05_000543"
     CDS             20965..22227
                     /locus_tag="U4B05_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049703.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            22307..23683
                     /locus_tag="U4B05_000544"
     CDS             22307..23683
                     /locus_tag="U4B05_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN2049704.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            23680..24309
                     /locus_tag="U4B05_000545"
     CDS             23680..24309
                     /locus_tag="U4B05_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049705.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            24321..24791
                     /locus_tag="U4B05_000546"
     CDS             24321..24791
                     /locus_tag="U4B05_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049706.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            24788..25270
                     /locus_tag="U4B05_000547"
     CDS             24788..25270
                     /locus_tag="U4B05_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049707.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            25267..25929
                     /locus_tag="U4B05_000548"
     CDS             25267..25929
                     /locus_tag="U4B05_000548"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN2049708.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            25916..26284
                     /locus_tag="U4B05_000549"
     CDS             25916..26284
                     /locus_tag="U4B05_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN2049709.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            26365..26817
                     /locus_tag="U4B05_000550"
     CDS             26365..26817
                     /locus_tag="U4B05_000550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049710.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            27469..27522
                     /locus_tag="U4B05_000551"
                     /pseudo
     CDS             27469..27522
                     /locus_tag="U4B05_000551"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27526..28125
                     /locus_tag="U4B05_000552"
     CDS             27526..28125
                     /locus_tag="U4B05_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN2049711.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            28141..28617
                     /locus_tag="U4B05_000553"
     CDS             28141..28617
                     /locus_tag="U4B05_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049712.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            28634..29536
                     /locus_tag="U4B05_000554"
     CDS             28634..29536
                     /locus_tag="U4B05_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049713.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            29609..29800
                     /locus_tag="U4B05_000555"
     CDS             29609..29800
                     /locus_tag="U4B05_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049714.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            29964..30551
                     /locus_tag="U4B05_000556"
     CDS             29964..30551
                     /locus_tag="U4B05_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049715.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            30551..31450
                     /locus_tag="U4B05_000557"
     CDS             30551..31450
                     /locus_tag="U4B05_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEN2049716.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            31450..32364
                     /locus_tag="U4B05_000558"
     CDS             31450..32364
                     /locus_tag="U4B05_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049717.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            32416..32631
                     /locus_tag="U4B05_000559"
     CDS             32416..32631
                     /locus_tag="U4B05_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049718.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        12389 a         4442 c         6637 g         9310 t
ORIGIN      
        1 atctttaggg cgtaagccct atttttttaa aattaagtga taacaaataa atgatttctg
       61 aaacaaacgt tcttactcaa ctactcgaaa cacacaagct aattaactag attataaagg
      121 agaatgcgta atgaaagaaa tcagtgcaaa tgaagctcat attccgcaaa agaatcaggt
      181 tggtatatat aaggtgcttc atggtgaagg aatttataaa atacagttac ttaccggtta
      241 caataatggt gcaaatggga agtggttaaa ggcaacaagt attcgtgaac ttagaagtga
      301 agtagaaaaa attaggcttg gtctcacaag tgaagaaata gctaaactga agggaaataa
      361 ttcttttgta attgaaaaag aaaaggaaaa acatctactt tatcgtgagc tgaaaattga
      421 agaatactat gaaattgatt tacctgaaaa attaagaatt attgatcaac gttcagaaga
      481 aaaatggaaa actgaaatat atccaagctt aaatgactac aagacatcag gaaagcccat
      541 gtataactac acaggctttt taacctacac acaatacaaa tatgcctttc gcaaattaaa
      601 aggcattaaa caagctgaaa ttgccctgta tgagatgaaa aaagtggata gattgaaatt
      661 gattcaagat ttagtgcaaa atacatggaa tcaagcgatt gtcgagctag aaaaagaaaa
      721 tgggaaaatt ttaactaata aatatctgat cgtaaatgaa attgaagaat taattggttt
      781 attttcatgt gaaaaaggtg aagtcttctt gaagtcatca ccaacctatt caattttgga
      841 cagtccgtat cagtttatac aggcaaggag atgagttaga tgtctagctt gaaagatgac
      901 tatatccaag caaaacaaga gtatgaaaaa atacagatgt tgaaatctga gaaagtcgaa
      961 ttatttttaa cacaaaaatt agcagaggat catattgcta acttagatgc taaaacttta
     1021 gaggaatatg atcggcagtt taatcagata catcacaata tcattttgct tgttgagcag
     1081 gcaaagcgaa ggtttgaaaa agcggaatat gatctattag atcaaggaat tgtatgcttg
     1141 gataagggat tgcgaagata ttatggtgca gagttaaaaa agcaaaatgt agatagaaat
     1201 gcactaaaac atgaaatctt agaattgata atgcaacgaa tttgaaggga tagaaagcaa
     1261 atgtcacaaa acattagtgc tgtaaaacaa ttaatagaag aagatttaga gaccacagtt
     1321 cagagattag cggaacgaat ccattttgca aattatcaaa ccatctctaa ctggtcaagc
     1381 aggaacagaa atccggaaga aattccaatg aaattttttt atcgaatcgg aaaagaatat
     1441 ggtgttgatc cgataaaaat tttcgagaag accatgattt attactatca tggaacacct
     1501 aacgaagtgt tagaacatct tttggaagag gcaaaagaaa aatcatcaat attagagcta
     1561 tcgcaaaatg acaaagttat tcaagtgaaa tgcgttgatc caacaggaaa gattattgtc
     1621 ggttttgata ttgaaagaaa atccaaaata gattttatta cacaattaaa aaagataaat
     1681 aatttagata ttctaacgct tagaccagat catgaacagt tgtttatgag ttttaaaaaa
     1741 caattgaaag aaatacaaaa aagatataac aaggtgtttc ctaatgcgtg aaaagctcaa
     1801 agaaatcaga gttttttgga taattaacaa agaatgtatc atcttatata ctcttggatt
     1861 tcttagcatg atcttgtttt ggtggttata tttaaaacag taattggaga gggtcaagag
     1921 gatttttatc aagataagga tgtggaaact gcatgtggaa taatagatta aaaacaggat
     1981 tgttattaat agttatttcc tgcgctatga tgattggtat gaggatacaa agggaacaat
     2041 cctattttga agtttctgct aacaacgtaa tagaaaaatg ctattacggt caacattatt
     2101 ggagtgagga agtcagagaa aatatagatc gtgaatatgt tcaaagaatt gtttgggatg
     2161 catattctat caaagattat cctaaaagtt taacgagtag attattttac agtgaaaaag
     2221 ataatcaaaa actctcagat ttgatgatga aaaaagtatg actattatta cgaaagagaa
     2281 acagagtgct atattcgagg ggaaaaagcc tacaacaaaa gtgactattg gggtgacaag
     2341 gcgaagcgat taactgcaaa ttttagagtg tatgaatcag agtttataaa cttgaccttt
     2401 cttaatagtg tttttattga ttatgtaatt actacgaaac gcattggaag ttattcaagt
     2461 acctacgcat ccttagttcc aatgttaaac tcgatgagcc gtttcttaaa agaaagagaa
     2521 aagaaagagc tacagtatat caaagaaatc atgccggatt ttgaaatgca cgaatggcaa
     2581 attgctttat cagattggaa attggcgaat aaggttcgta gaataacacc ttttcaagcc
     2641 aagcgctttt tgagagctat gaaatttgtg caataaaaaa agccaccaga caagttgatg
     2701 gagactaaca caattaccaa cagttgtaat tgtgttagct tacaaattaa ttatggtgtt
     2761 tagtcaatcc caaattgtga aatggcacca ataattggaa aggaaaaatg aaatgtctga
     2821 agatttacga gagctgaagt tacgaaagaa gcagtatgat cagtattttt ccggtttgtt
     2881 gaataaacat attgaagaag gacttgatca tgattttgga aaagatgaat attgtttata
     2941 ttgcgaacgt tacaatgaaa aaatgtctga atatctacag ctgaaaaaag agatacagaa
     3001 attagagaaa aaagtacatg cttggaagta taaagattcc atgtctggtt atgttgaatc
     3061 tcaaaataag ccaagaaaaa agaagcaagc gtatgttcta aagatggtaa atggggaaga
     3121 gttaattgta ttagcgtaca gtgaagaaga ggttcgagaa ctcgttgaaa cacctgtcag
     3181 gaaaaaggtt gaaacaataa atgagataga cgacagccaa tacaatagca tctatattga
     3241 aacaagtcat ggtggatata agaaattagc aactttcctt agaagttcta aagttgggat
     3301 aatttctaaa aaagacataa gtgctgttac cgatagtttt tgtactttat ctgttcctaa
     3361 agcttatttc caatacgata gtaaaagatt tgaacgctta gagaaattct gtagaccgta
     3421 tactgtattg aaaactaaaa aatttaataa aactaaaaga gtttctatac agttaccgac
     3481 tggtcaaatc tcattaggtg aaaaagacta tctgataaaa gtaggaaaag agttaattat
     3541 aaaagtaccg gaacacttat ttaaagaaaa ttttgagata cgtgaaacct tggtatggaa
     3601 agaagaaaaa tagtttggaa gacatagaat ggtataaggg gaaattgaaa tggcagtaaa
     3661 taaaatattg caggtactaa tcatagacga atttggcgga ggagaatggt ttgatgtgca
     3721 gtccatagac tttgaagaag aagtcgtagt tatagaaaat atttacggcg aagtatattc
     3781 atattcgctt gaaggtgaga atattgagga tttacggttg attggtgagt agaaaaaaac
     3841 agaaatatca aaatggagat aaaaattaat agcatgtaaa gatagtcatg cttgggttaa
     3901 aaatgttaaa attcaaaaaa atgagtcaga agggataaaa gtatgaaaag agaggaatac
     3961 aaacaaagat taaacgaact gcttgaagag gacgaaacat taactcatgg ttcgcctgat
     4021 gaaattctat acatgattga taatatggtg atctttggtg gatatgaact tggtaatagg
     4081 tcagttgatc ataatatttt agaatttgat gatgtatcat gggaagaaat tcttgattgg
     4141 ggaattttgg cggtacctga aactaaaacc tatattagtg acactatggt tccttttttt
     4201 gaggaattgg attataaaag acttcccaaa aatgaaaatc atatactggg aggaaactaa
     4261 caatgttaag tgacttattt attttactga tctatctagg tgtaagtgaa attattgcgt
     4321 attttttgtt tgtcattgct aattatggta tttccagtgc aatcgttcaa atgtttagat
     4381 taccagtaag tgaactaaaa aaatgatgag attatcagca tatcttttaa tgtttttgaa
     4441 tgtggtcacg ttaatctttg tagtaatcgg tgtgactttc gatttaatgg aatagtggga
     4501 aatctataat ggaaaaaaga gacacgaatg aacatatttt ttgaatcaat attgttgatg
     4561 gtttttgcga tcagcgctta ccagaaatct aaaaaagagt aatgattgtt taatcatcac
     4621 tctttttttg tagggaaatt gaaatatgtg gagcttgtat tggtttagtt aggattgctt
     4681 ggctataagg agctagttct ttatcaaaac catcaaagta ggttttcagt agcgatttaa
     4741 ctgatcgctc atcttgtttg atttggataa tttcagcatc caaatcggtt gtgagatcag
     4801 gagcttttga tgcgattacc tgattttttt tatcttccaa ctctcttagg ctgtttaaca
     4861 agttgtaaaa agaatcattt tgattgttca ttccaacgtg agttgcaatt ttaacagaag
     4921 tttcgtccgt attgtcatca aaagaactca ttggaatatc aagtagtaac gaggatgttt
     4981 cttttactga ctttttcttt acagcatttt ttgtttttcc atcttttttg ctctttactc
     5041 gagatgtttt tggagctagt ttaggtaaat cacttgtttt atcagtcatt tattagcttt
     5101 tcctttctat taatttatgt tattatatta tcataataac ataaaaaagt acactttttg
     5161 ggggcgtagt tattgaaaaa atctaagaga aagattgcca cattgcttat gttaatggtg
     5221 caatttacag ggattgcaat gagtgtaatc acaataaatc cgcaagtgat acatgctgat
     5281 gggatagcta cttatgatgc acaatttaac tgtactactg aaggtagtgg gtcaggaaca
     5341 gatagcgatg gaaacgatac tggaagtggc gctgtaacag gtgatgtagc caaaaatatt
     5401 gaagatattt acaaatacat gaatggagaa tatggcttat caggtgctag tattgcaggt
     5461 attttaggta actggaaaca agaatcaggt attgatcctc gagcagtaga agggtattac
     5521 ggagatggac agagagaagt tgacttcatc aaacaattta ctgatggtct gatgggtatt
     5581 ggctatggtc aatggacagc tgggcgacat acagcattag ttgattggtc taagaaaaat
     5641 aatggcggtt cttggtggac tacagaagcc caattggact ttatgttcaa aggtgatggg
     5701 ggattcgtag gaatattaaa agattatgct ttaaatgctt ctgatgatgt cgtagaaaat
     5761 gcagtaactt ttcataaaaa ctgggaaatt tcagctgatt ccgaatcagc tgtaatagct
     5821 gtacgtggtg aaaatgcaaa agaaatctgg aagtacatga aaagcaaggg tatggacggt
     5881 gcaaaagatg aaagtaagat caataaaata tccggaggtt caagtaccgg agatggatcg
     5941 aataattcat caagttcagc atcaggaaca gtagaagatg cctgtgcaac tacggatgac
     6001 gctaattctg gttctggatc aattgggaaa gaaggaaaaa ttaacggtaa aaaaggtcaa
     6061 gtaatagcga atatgactta tgaagaagct ttgaaaaaat atgggaaagc gatcacgcta
     6121 ccaaaattca aagaaccaga ttggtcaact tcacctttta acggaatata tcaaggtcag
     6181 tgtacagagt tgacgtgggc ttatatgagc caattatacg atgggaatca accgactact
     6241 ggaaatggtg gttatgtatg ggaaagctac aaatcggcag gagcaaaaat aacgaacaaa
     6301 ccaatcgtag gttatgggtt tagtgcttcg gatggatggt tatgggctgt tggagatgca
     6361 gggcatactg gtgtagttat agcggtgttt gaagatggat catttctatg tgctaacttt
     6421 aatgttccac caacccaagc tccaacaagg ggagtttcgg taacgttgat tgatggagtg
     6481 gacggatcag ataatgtgca tttctttagc ggtgtaggaa aagctaaatt tgaaatcgaa
     6541 aaataaattg gaatgaagga cgatatagat gaatagtaaa gtgaaagata caattatcat
     6601 ttctttgctt tctggtatgt tattagcact gcttgttgga atcctattct tcaataagca
     6661 gaatcaggac atagaagctg aaaaacacag tgtgcatatt gaagataagc tgacattctt
     6721 taaagaggtt aaaccaaatg aggtggatgg attacggaaa gatttagaaa atgatattca
     6781 agaaatgttg aatggatcag ttcaaaataa gtttgcagat caatcaggtg aagaattttt
     6841 gagaaaacta tttattgtaa caggtaaacc accaattact gataagagta gtgagaaaga
     6901 aaaaatcgag tactataagc cttttacttt taagctaaca aactttggaa ttaagacatc
     6961 tagtagtggg ttgtatgaag cattgaccga ttttgaagtt caatacaatg gaaaaaaagt
     7021 gtatgatacc acattaacca tcacactaaa taatgataag aaagtgactg ggggtgcttt
     7081 ttatgcaaaa tcatgaaatc actactgcta aaaaggcgat cggaatactt tccataattg
     7141 tgatcctttg ttgtgtggta ctatttcaaa agaaaaatgc ttatgataat aatgtcaaag
     7201 aattggaaga ccttaaaagt aaacaaatcg aagtagtcaa gaataaccag aaagtgaaag
     7261 ctatgcagga agctcaatac aaagaattgg gaaatgctga aattaaggaa caaagtagga
     7321 aattctttaa tttattttac aactggaatt catgggaaaa atatacgaag aatatgaagg
     7381 aaatacaaaa agaatttcct cagatagtaa agtctagtcc tgttgatatt tctggcacga
     7441 tggtaggcac tggtaatagt ccaatatcta gctatactgc tgattactat acaacaaatc
     7501 aaaaaggtaa aatggttgaa ttgattacac agacaagaaa tactccaaca acagcaacaa
     7561 caacaatttg gcgtggagta acaagcatag gagaaaatca attgttttat ttggaggatt
     7621 tagatagcta tgtaagagca gagtaaaata aaaaaagagc taacggtacc aacgttagct
     7681 ctttgtgctg acgatgataa caaaacctac cgaaaaagga ttgtttcatc acaaacacaa
     7741 acagaaaaga attgataaaa aacccgaacc tcagaatttt tattaatcaa ttcatttaga
     7801 ttataataaa ttgagctgaa cattacaata cgtatatgtt gtaaaacgta aaaaaagtca
     7861 aaagtaaata ctttcttaaa aagtcaaata aaactattaa agacaatata tatggtttat
     7921 ttgacataag aaaatggtct agatgataat tgtaatatag aattgtagac ttaaataaag
     7981 cataaaatac cttttatgat aaggtatagc tcttaatttt aagcagtttt agatataaga
     8041 ttgaaagtaa agtgggggag aataataatg gcagatcata aattagaaaa gttattagaa
     8101 gaaacgtgga atccaaaaga atttagtgaa ttttttatgg agaattttga aactgactta
     8161 gcagtgattg ttaaagatgc tcttagagag caagggtatc ctgaaactgc aaactatata
     8221 aacattaatt ttactttgta tactgaaaat aaaggaactt gggatttttg ggctacttta
     8281 gcaaataagg agttaagtga taaatcagac acaggaattc gtaacttttt tgaatcaaac
     8341 agagatgatt atatgtatgc taacaatcaa aataaattga actttagagt agagtttgat
     8401 gaaacacctg aagaatttat agaaagacaa ccgcctaaag aaaatgtagc aaaggtattg
     8461 gaagatcgtt ggaattcaga tgaaatagtt tcaacaatta gcgaactagg cggacaatat
     8521 gaaccgctag tagaagcggt tagagaagag ctaagactga ataagtttcc tgatgttcaa
     8581 aatattgatg tttctcaaat tgaaataaac gtcaagatca cgaataaact tgattatggt
     8641 agttgggcag atatagcatt agaaaaatac atctactcaa cgttaaaaga gtttatcgaa
     8701 aatagaatgg atatcatgta tctacagcat ccacagtatt taaactttgg tgttgaaata
     8761 gctacacctc ttgaagaatg gaaaatggag caagggcttg actagccttt gctccatttt
     8821 tttattttac aaatgaatac agttcgtcgt ttttaccaag aatgattgtt tgcccaactt
     8881 tgagattgtc aggtgatcct tcagcggttt gtaaggagaa cgttccattg aacttatcgg
     8941 ataccgttat aggtagcttt aattctgatc cttcctcatc tttgagaatt gctgtttttc
     9001 gttcatcaaa ataaacagtc tttattaaat aatttttcgt agtgatctca gcagatttgt
     9061 ctttttttgt agtagtacca gtttctttag aattagaggt attttctacg actattccat
     9121 ctttggcatt tgagcttgtg tttatttgat tgaatatttt cagcgacaag ataacgctaa
     9181 gaatagtcaa tatagaaaca agtgtaatta atactgataa tttccttttt ttgttcattt
     9241 tttcttaaca tccttttttg ttttgattag ttagatattc cttatctaac aagccttttt
     9301 aggctattaa atgaagataa accccaaaaa aatattgcaa ttatgacagt gcaatggtac
     9361 tattattata caataaattt tgcgaaaggg gtaggtctaa atcgcaaaca aaaagaaatt
     9421 gaacctagac aaatacaact tgtataccgc aagggaagca agtttgattt tgggtaaagg
     9481 tagaaattat gttacaaaca taatcaacta ttatccaaat cgtttgccta aaggttcgtt
     9541 caagaaagtg ggtgacaaaa cctatattat aacggaagaa ggaatagaag cattgcgatc
     9601 acaaacagaa aataaaaaca aaaagaagga atgaatatga agagaaattc gcttggtatg
     9661 gttttattgg catcagctgt acttgcgggg ttatcagtac cagtaacctc acatgctgat
     9721 gaatggactg caaacacacc ggatcaaatc caaattacaa caggacaatc aagttatgcg
     9781 ttcaaaaaag gagatactat ttggtcaact gtagagaagc ttaaaagctt aggttacaag
     9841 actactgttg ctgagtttgc cgaattgaac aatgttgatt tggcaaaagg agaacaacgg
     9901 actatcccga ttggacgaac tttcaacatc agtaaagata cattaacagt taaggaagca
     9961 gatggcgaag tagttagtca aactgttcaa aaagataaac ctgatcctat tgaaaatacg
    10021 gagcaagaga acaaaaaatc cgagactaca aaagaatcgg aaccaacagc aaacacttct
    10081 gataagacag aaacaagtgc ttcaagtgtt gatggagcaa gttcatctaa tacaaataat
    10141 tctacaagtg agaaagaagc atcacaggga acaagtgaaa gtagcactac ggaagctgat
    10201 actggaacat caacaaataa ctcacaatca acatcaaatg atgcaaatga atcaaccagt
    10261 acatcttcta gtgatcaatc acaaacaggt tcgagtgatg aaactgacag ttctacttca
    10321 ccaagtaaac ccgaacaacc aactaatgtt gtgctaggcg ttccatttat tagtcaaggt
    10381 aacacaatgc tttgcgaagg aacatctttg ctagaagcgt tacattataa aggtgttgca
    10441 acaaatcaag atttaatgac ttttgttaaa tcaatgccac tatctcctga caataatcca
    10501 aataatggtt tttctggtga atggagacac aatgtggatg gaacttatca aggtatgaat
    10561 ccggctcctg tagtttcatg gggtgttgct aatggcggaa atgttgtaga tatttctggt
    10621 tcatcggtac aaggattaaa agatgagatt gtaaatggaa atcctgtagt agcgtgggta
    10681 acttatgcct ttgaacctgc tcaacataaa ccaatgtttt ggggcgatgc tatttggaat
    10741 cgacatgtag tagccgttga tggatttaaa gacggttttt accatgttgt cgatcctgtg
    10801 tttggggcaa gttggattaa tgcaggatca tttgaaacag catataacgt atctcaatta
    10861 gcggtttcgg tacgttaata aaagatatcg tttatatact aagtagttat atagtcttta
    10921 tttgtccttg cttaactact tagaaaactt gttgctcatg gtgggtagaa ctaaaagggg
    10981 tgatgctatg aatactagag cgttgaaaca gcttgctttc aacgaaaaaa atgattaata
    11041 ggtgtggtga ttttaactta ggaatgggcg cgaaggtcat taccacaccg acatatcgtg
    11101 gctcattttc ttcgcctaac tggtataaag aaggagtaac gagagatgaa gcatgaaaaa
    11161 acgataattt taaaatcaaa aataatttat tgttttgcga ctttagtaat gatgttatct
    11221 acgcttataa tgcctttttc agcaataaga gctgaagcag atgtcacaaa accgacaagt
    11281 gcaaaattta tagaaactgg cgtaaaaaca gacggttata ttagggttaa tatgccgaat
    11341 catccaaatg aatggatgat ttcaagtcaa tttaaagatt ctcatggaaa tattggatat
    11401 tgtatggatt cagaattgcc atccccaaca ggaagtggag ccggaagctt gaagtacaag
    11461 ggagctggca gtgatgagtt ctatcgtatg tttaaaggtg gttttccatc aaaaacagca
    11521 aaggaactgg gtgcaggaaa tgatacagaa gcatggtatg caacacaatt agtttcatgg
    11581 gttttagccg gaaactttaa agtttcacag attgtatggt cacatccaaa ccataccgcc
    11641 gcagaaacag ctcgtgtaaa aaaagcattt gaaaaaattt atgactatgc taagaatgga
    11701 aaagatacgc ccaacactga gttttctatc acagcttcta agacagctga cgaaggaaaa
    11761 tatcacactt tcacatataa aacagcatca aataagactg gtaatgcgaa gctcactttt
    11821 acatcagcaa aaccagctgg tatgaagatt tatgatgcag atggtaaaga aattactaac
    11881 aacactgtga aattaaattc aagttttact attaaagtac ctgtgactac accaagtggt
    11941 actttgtcat ttaaaggtac tgctaatgtt agcacaacca atccttttac ttttgatgga
    12001 cgaggtgtgt atcaagatgc cgtagttatg atcactactt cagaaacaaa ggactctaaa
    12061 tctttgagtg ctaaatggac aagagctgtt ggtcaggtaa cagttaagaa agttgatcaa
    12121 gatggaaatg ctttaaaagg agcggagttt actctaactg acaataacaa tgctaaatct
    12181 gttaagacaa ccggagatga tgggttagct acgtttgatt tgcaacaagg cttaacatac
    12241 agcttagaag aaacaaaagt tcctgaaggt tatcaaggtt catttaaaaa ggatggtatt
    12301 actgttaata ccgatggaca agaatttagt tacactgcta cgaacacaat taaaaaaggt
    12361 aaagtaaaag ttaaaaaggt tgaccaagat ggaaaaccat tagctaatgc tgaatttacc
    12421 ttgacggatg attcatccca aacacaagca gaaaaaacag atgaaaacgg acttgcaagc
    12481 ttcgatattg tagcaaacaa gacctacagc cttgaagaga ccggagtacc aactggttat
    12541 acaggcgtat tcaagcaaga aggaattacc gttaaagatg atggacaaac ttttgaatat
    12601 acagctacaa acactttgaa aaaaggttta gtaaaagttc ataaagtcga tcaagatgga
    12661 aaacctttgg caaatgcaga gttcacttta accgatgaaa aaggtgcaac acaagtagaa
    12721 aaaacagatg aaaatggact tgcaagcttc aatattgtag caaacaagac gtatagccta
    12781 gaagaaaccg gagtaccagt aggttacaca ggtgcattta agcaagaggg tatcaccgtt
    12841 aaagacgatg ggcaaacttt tgaatataca gctacaaata atttgaaaaa aggtgtagta
    12901 aaagttcata aagtcgatca agatggaaaa cctttagcag atgcagaatt caccttaacg
    12961 gatgaaaaag gtgtaaaaga agttaaaaag acggatgcaa ctggtctggc tacgttcaac
    13021 attattgcga acaaaactta caacttagaa gaaacaaaag taccaacagg ttacacaggc
    13081 gcattcaaac aagaaggaat taccgtcaaa gatgatggac aagtctttga atatacagct
    13141 aaaaacactt tgaaaaaagg tgtgatcaaa gttcataaag tcgatgatca agggaaagca
    13201 ttagcaaacg cagaattcac tttgacggac gacaaaggtg taaaagttgt acaaaaaacg
    13261 gacagaaatg gcttggcaac atttaatatt gttgccaaca aaacgtacaa aatcgaagaa
    13321 acacaaaacc ctaatggtta tacagggaaa tggtctaaag aaggcatcac cgttgctgac
    13381 gatggacaaa cctttgaata tacagtaatt aatcaccttg tacctgaaac agtaagaaca
    13441 ggcggaatcg gtacatcagc cttccttctt gtatttctaa ttgcaggatt gagtatggtg
    13501 ctactgtatt atcgagaaca gcacaaagca aaataaaaaa gtgttaatgg aaaagaagta
    13561 aatatgaaaa aagaaggctg acgtatgaaa tcaaaaaaaa ttttttcaaa tttaatgcta
    13621 tttggtactt tgctaggagt gggattgggc gcaagctcaa tctcttctag ccttggacta
    13681 ggggggcaag tagcatatgc acaacaaaac tataattatc aaggaactgg aacgattcat
    13741 atccataaag taacaaatac taatggggat gggaaccctg tctcagatat tgataacaaa
    13801 gggaaagaaa tagctttacc aaatggtgta aaacctcttg aaggcgttga gttctcttat
    13861 gtgaaactta ctaaagatga agctaaatcc ttgggatgga cggatagtga tactacaaaa
    13921 ggtcaacata ttgatgatgc taaagcacga ttattactaa acaaaaaata cagtgaagta
    13981 agtagaatga agactgattc acaagggaat atagacatac ctatacaagt aaatacagac
    14041 gatttaacag ataacttgta tttgattgtt gaaacaggaa cagtcaaagg tgtagttgaa
    14101 gaagcgtatc ctatgattgt taacgtacct gaactaaacg gatcagatga tcaatacttt
    14161 aattatgatg tacatgtcta tcctaaaaac tatttccaat taaactcagc agagcttgat
    14221 aaaaaagacg aagatggaaa ggctcttgcc ggaactgttt ttgaattgcg aaaagatgat
    14281 ggatctgatc aggttgttgg aacttataaa acagacagca caggtaaatt aactgtggat
    14341 ggtttggaat taggtgacta ttatttccta gaaaccgatt tgggaacaga tgctaataga
    14401 acaacctatt tactagatgg taaaaaattc aaatttagcg ttaaacgtgg ggagactgct
    14461 aaagtagttc ttgatgttac caactacaaa cgacctgtaa ttactaaggt aattgataaa
    14521 gaaaccgttg atactggcga agatgttcaa tatacattga aaccatcaat tcctggtaat
    14581 attggtaatt acgaaaaata tgttgtcaaa gatgttctag gtgcagagtt aacttatgat
    14641 tcatcagaag tgatcgaagg aatgacttta gatactgatt acagtatcga acgtgacgga
    14701 caaacgacaa actatgtctt tactgaatca ggtatggata agcttcagaa acaatttgat
    14761 gcaggggatc gcttctttac aattttgatg aacgctcata ctaatagtaa agctaaaatg
    14821 gatacaccac ttaagaatga tgtttcaacg atctacaaca atggatatga acccgaagac
    14881 gaaacaccag ttgatcacgc ttatacaatc attggtgatc accaattcat taaaacagac
    14941 ggagatacaa gcaaagcatt gagtggagca aaatttgtag tgaagaacaa aattgatccg
    15001 aataaagtta cttacctaaa acaagatcca acaacgaaag ctgttacttg gattgcagat
    15061 aaatctaaag ctactgaatt cgtttccgga tcagacggta agttccaaat tgaaggtttg
    15121 caatatacct atgatgtaga ttttgataaa caaggtaatc caacaatcac agataaaaaa
    15181 atcaatgatt atgctcttga agaagttgtt gctccttcag gatatacacc aactagaaac
    15241 gatgttccat ttgtggtatc gaatgatgat gcttctagcg aagtaacaga catcaagaac
    15301 cttaaaacac ctgaaacagt tcgtacaggt ggaattggaa cagtagtatt tctagtggtt
    15361 gcaggtgtgg caggaacagg gctatatgtt gtctacaaac gagaacaaca caaagcttaa
    15421 tgaaataaaa ttccctagag tgtttttggc tctagggttt ctttttaaaa agtgtgtgaa
    15481 aagtaggtat gagcatgaaa aagaagaata tcaagttgat catcttaaaa attgggatgt
    15541 tgctatgttt aggagtaggt ctatatcctg taattactca ggcttatacc tactaccaaa
    15601 ctcaacaaac actaaactcc tataaggaaa aatatgatga agcttctgaa aaggagaatg
    15661 aagaagtctt caagagatat tcagaagtta ataggaaacg aaaagacaca gaacaaacct
    15721 cagtcacgat tcctgaagat aataacaata ttccgtcaaa tggaaatctt cagaaggata
    15781 aattaggtga attagtgggt gaaattctta taccaaagat taatgtggat ttgccaattt
    15841 atgatggtgt aaacgatttc caactatcag caggagcagg aatattaacc ggaactgatt
    15901 atccaactgg tgggattgga aaccattctg ttatatcagc tcattcaggc attcctaata
    15961 aaaaatattt tacagattta gagttgctca aacttggtga taaatttttt attgatattt
    16021 cagggaaaaa acttgcttat caagtagatc ggataaaaac aattctacca accgaatttg
    16081 aggatttgct tcctgtggca ggaaaagatt atgtaacact tatgacatgt acacctgttg
    16141 gagataattc tcataggtta ctcgttagag gggttagcat accttaccaa aaaagtattg
    16201 ttgagaaaga aaagaagaaa gaagatttaa aggtaaaggc gcagatgaac tggtatccta
    16261 tcgtagttgt aggggtttta ttaataatag ctgtgatagt cattattaaa aaaatagtag
    16321 caaagagacg aggaatgaaa aatgaagaat aaaatactac tttttttgac tcttatttgc
    16381 actttggttt taacaattta tcctgcaaat cagctctatc atcaattcaa aacaaaagag
    16441 aatgtggcgg aaatccaaga gaaaattgag aaaaaacaaa ctccttcaaa agaggaagtg
    16501 gcaaaaaaag tcgaacaaat aaaatcgaac agcggtaacc aaggattaga caatactcag
    16561 aaaacagata aaggagtaga caatactcag gaaacagacg gtggatcatt tgatattcgt
    16621 gaagctgaag gaaaggtcaa tccatacaca ggagatgttt tttcaaaaaa tcaaacgaaa
    16681 gattttgaac catctaccta ttcaggagcg gttggttatg tctatattcc aaaaataaat
    16741 gtgctagagc ttttgtttct aggcgcttca caggagcatt tatatagagg tgtggcacaa
    16801 atgagtgcta ctagtctacc tttatcaggt aaaggtcatc aaacagtaat agcaggtcat
    16861 cgtggcggtt atacgtcacc acaatttctt tatgttgatc aattagtagc cggagaccac
    16921 atttatatag attatcttgg tgaaaaagca gtttatacgg ttttccgaac agacgttatt
    16981 agaaattacg gtcagaattc aacactgact gatattcctg atttagatat gttaacactt
    17041 tatacttgtc atccatatcc tcagaactac gatcgtttat tagttagaag tctaagagat
    17101 gaaaaagcaa aatacatcac gccacaagta acagttggca ataccgcata cgctgatgcc
    17161 attgatagcg agtccgtttc agataagaca gaaactgtta catcttctaa tgatttattt
    17221 gaggatgttg atcgtgccaa caaggaatta gatggaaata atgatcttaa tattccggaa
    17281 caaggtagcg tagagatgca agacagaacg tttgctgaaa aggcaagttc aatatttaca
    17341 ggaattacca ataaacatca gaaaatgctt ttagtcattt ggactgtaat aggagcatta
    17401 gcaagtctaa aggttcttat tcttttaatc aaagagtttc gaaaagcata atgaagatta
    17461 taaaaattga tagagtacag taaaaaggga ttgatactat tattgatctc ttttttattt
    17521 tgaatagagc tagattcact tttaaataaa atcaaaactg atcttaattc taaagaaaaa
    17581 gtcaagaaaa aaatacaaca aattttttta tcagaatatt tatcagaata ttattagaaa
    17641 agataaactc aaaagcttaa tttaataggg ttattggttg tatttagaaa taatgtatgg
    17701 aaaagctata aatcaataga taagatgtag aaaatcttat ttgtgaatga aaaacaagtc
    17761 atcgtaaaat attaatagga ggtgtagtaa tggtgggaat atcaaaaaca gctttagact
    17821 cattagctaa actatttagt agcaggtttg atggtgcaga atttgttgaa tatctaagcc
    17881 aaaacgaaag tggaaaaact ctgatcgaaa caatgaaaaa agactatttg aatgaaaatg
    17941 actttccgac atggacgaat attgaagatg ctacatggga tgtagtatgt acgatatatt
    18001 ctccttgggt aacgattgct gattttgttt tagataaaat tggaggggat acataccctg
    18061 atgccaaatc tcttgctaca agtgacttta aaacgttcta tggttatcta ataggtctaa
    18121 taggcaatgg aatagatttg aaagttacgc caaaacaaac acctgaagaa attgaaaaag
    18181 aaaaaggaga ggttcaaatg gcttatgcaa atgacagaga aaaagtaaaa ttattggaag
    18241 gtaaattatc tgaagaagaa cttggaaaaa tggtgcaaga aatcaatgaa gacagcaact
    18301 attttcctga attgaaggta tatcaaaatg acgaagattt ctttaatgaa atgtttagta
    18361 atagtccatg ggaagcagtg agtaaaacaa atttaggaga atggaacttt caagatgatc
    18421 tagttgcatt tagcaatgat gggtatttac gatcatattc agaaagtgag tatagagctg
    18481 aattaaaaac gtatgaaacg gatattctga tgagctattt aaatctatta gagagtggtc
    18541 agggagagtt aagagataaa gttgaagaac tattaaaaga agagctatag ggattatttc
    18601 aacataaaca caagcagaaa actactaaac aaaacgtacc atgcaaaaag tcggttaaac
    18661 cggctttttg ctctattatg agaggagatc aaaccatgag tgcaacaacc gatatggctt
    18721 tagaagcagt aataaaagat aatacaggaa atgtcgaaaa cgtagaagac tttagcgata
    18781 ttgcaatcaa tctttcagca tggatttcag ctgtaggaga agaaaatatc gaatttgaga
    18841 tcaagaatat ggatgactta aaagcaaaat ttccttttat cgcatcgaaa ctaagtcaag
    18901 ttgccgagga taaatcgtgg aatgaattag ttagtttttc cgaaaaaatt tatgatatta
    18961 cgcaagaaga tgagtgtgtt tcatgggaaa tgaataatca atacaagaaa attgacgctt
    19021 tcgtcaaatc acgtatagaa ggaacagctt ttgagtttga aggtatagat accgcagata
    19081 attggttaaa taaagaatcc tttattaaaa gtatagcgga tgatcttgaa aaacaatggt
    19141 ctaaggaaga ctatgcagag tacctaaaaa cagaaagaga tacagaggtg aaagaaaaac
    19201 tcactgaggt atgtttggat gaacatattg atcccaatat cgtaaaacaa gaagactatg
    19261 aagtggttat tgataaagtt cctactgatt ttgaggaagt tgtaaagtat ctagttgaag
    19321 aagcattatc gcaatactct tcagatgagg aagtggacga agaaactgtt cgtaccagag
    19381 tttggcaact ggtttttgat gacaaagatt ataaatattt gagtgatcaa aactcggtta
    19441 attggagtgt acgattatta attgatcaaa atgatattga ataaaataga gctgaaaagc
    19501 tctatttttt ttgttaaaaa aactagctat tttattaaaa ataatttggt tcaaatttta
    19561 tttttttaac ttgataattt cttgccatcc ttttttaaat tggctatata tcaaggttta
    19621 tagtgggaat aaataaaaat acatttgaca aaatttacaa tatagtttat tatatattta
    19681 tcttattatg ataacacaat aaaaggaatg gtggtatgaa aaagaagaag tttagtcgat
    19741 cgttgttagg aattttggtt ttagttgcgg ttggtcttag tggcaagggt tactttcaga
    19801 ataacagcac tcaggaacat gattttcaga tcaaaagtag tgttcagata ggtaataaag
    19861 cagacagtga atcaaaagaa tttttaggga tacgttatga tccaaacaaa tatccaaata
    19921 atatcgtaaa cattaatgat gggaaatata cactgacaaa agaagaccat gaagcattat
    19981 cagaaaaagg cagtcaaacc ttttgggtaa attatgcaga tttagatcaa cttggaagag
    20041 caggagatgt tgaagcgttg atcacctatg atgctgtaaa ttctcactca tctaaaaatg
    20101 tggaacgtcc atctttcaaa agttctgtcc atattgcagg agaatacaag gatggagtat
    20161 ttgatcaaga gagagatact tgggtcggca aagattcaaa caatgaaatc actcagctag
    20221 aaggctataa aggttatctc tataacaaaa gtcattcatt agcatggtcg ctaggaggag
    20281 atatggaggt acacaattta acacttggaa cgagagcgca aaatgttggg aaaaatgatc
    20341 agaatggagg gatggcttac tcagaaacga ttgtacgtga tgcaatctat aaaaataaga
    20401 atgaaaaagt attgtataaa gttgatccaa tttataaagg gaatgaatta gttcctcgtg
    20461 gttctcatgt cgaagctttc tcagttaacg ataatggaaa aacggtaaac cttaatgttt
    20521 atgtatttaa caatcaaaaa ggaatcaaca tcaactatca aaatggtact tgggtaagag
    20581 attagaaatt gagaaggtga agaattggaa acaataagca tcaatggatt agataaatca
    20641 gttgcaaatc gaataaaaaa aggaaatggc tactatcttc tgaagaaggg taaatattca
    20701 gttgctagag agttagaaac aatgagtatc gtgtatgtag ctaagaatca ttttgacgta
    20761 gaacaggcta tttttgatcc agattctgaa atagggaaat gtgttgaatt atcagagaaa
    20821 actgttagta caaaaggggc gatggtcatt gagagagaaa tgttagacga aatcagagta
    20881 atcattccta aagatacgca gatgtgtgag gacagcataa gtgacgatga accaaaatca
    20941 gaaagaaaat aaggagatat tcagatgagc gaaaaatcca ttgaattaaa ggaatataag
    21001 cgaagcgatg gagaaaaata ttatcctgta gccagttttt ctaaatcagt atatctggtt
    21061 ggacgtgcat tggtagttgg tgcaattggc atatatattt tgcattgggt aacaaaggtt
    21121 gtacaactgc ttgtagatac cttagtaagt attcttgtct cgttaaatga tgttaacgga
    21181 acagatgatt taggattaaa agagatgttt aaagagttac tcaatgcgtt ttttcccaat
    21241 tatttaagtt attcatataa tgtacaaagc ttttttgcaa agctacactt ggtagacatc
    21301 tctattttag tattagaagc aggaacggta ttctttgttg gatatttggt tactttgatg
    21361 ttcaggcatc actatggaga acaaagacca ttttttgatg atatggaaag tagaaggttg
    21421 aaaagagaag ctctagaagc tatggatgtg aagtatgatc caaacaagat tataggagat
    21481 gaaacgaaca acaaaaaaag aaaacgaaac aaaacgcaat tagaatccga tgtgctatca
    21541 acgcttaaaa atatcaaaat ttctgttcat acaagacgtg atctaagtgt caatgaagat
    21601 attcgagaat atatcgtaaa aatcccgcaa ccaaaaggga agaaagttag agaaacagtt
    21661 ttacgacaaa ttcaagattt agggtcgatc ctgacaagag caacaagcgg acgagccaaa
    21721 tttggtgaaa aagaagatga agtttcaggc gattatttca ttttccgagc atcagtaaat
    21781 attgaaagag aacgtaagga aagagtaagt accaaaaaca aagaaacgga aagagcaaca
    21841 tcttcaatcg cagtttcaaa ttcacaattt tcttttccaa tcaatctctt tgccgataat
    21901 tccaaaaata tccaaaacca aaccgaaaaa gctagaaaat atgccgaaac tcaaaagcaa
    21961 tctttagaac tttatctatc ttctgctgac gtacctgcaa gtctaacaga tatgcacgtt
    22021 gggaatacat ctattgaata tttgtttact ttatctcgta ccagaaaaat tcaactaagc
    22081 tcaatgaaag aagaaattga aacttacttg catatatcag gtgtaatcgt gcggatcaac
    22141 gcagggcgta ttcaaattac tttaccatta ggagaaaatg atcgtattcc aattgatgta
    22201 ccatcaatga tcaatagcgt gttttagaag acatcacaaa tagaaacaga aaatacaaac
    22261 taccagttca caaacagaaa agtaccgaga aaagtgagga atagaaatgg ctatgaaatt
    22321 attagagaaa gtcaaaaaga gagccgaaaa agctgaatct attttgctag ataaagaacc
    22381 taatataaag cttaatggaa aattcagtgt caatatgttg aacacaaagg ctaaagaccc
    22441 aacagatgct atttttggaa ttaagcctga tggcacaatt gctcattttt gcatcagcga
    22501 aatgccacac atgttggttg caggaacgac aggatcaggg aaatcagtat tactgaatga
    22561 aattcttgtt acagtaatgt gtcactcaac gcctgatgaa ttaaaaatag gaattgtaga
    22621 cccaaaaagg gttgaatttg gaagatacaa aaaattgccg ttcatgttgg ctgatccaat
    22681 tacagatatg gatgaagctt atgatttttt tgaatatctt gtgatcttga tgcatgaacg
    22741 ttacaaactg atggaaaaag cagggttaca aaacatcacg ctatataacg aatatgccga
    22801 aaaaaatgga ttagatagat ttccctatgt gatcttatta gttgatgaat attcacagtt
    22861 ggttggaaca cataaagaag tagaaggttt gattgttcaa ttaggacaaa tggcaagggc
    22921 agcaggtatt catgttatct tagcaacaca atctcctcga tcaacagttg tcactggtat
    22981 tatcaaagcc aattttccgt cacgtgtttg ccttatggta gcaagtgatt tagaaagtag
    23041 gattgtatta gatgaaggcg gtggagaaag tctaagtcca aagggagata tgcttattaa
    23101 gcttgttgat ggaagtatgg ttagagcgca gggagcttac atcagtaaca aagaaattga
    23161 aacaatcttc aatcacctta gaaatactat gccagaacca gaatatgttg attaccgagc
    23221 aatcgtagct gaaactcgtg gtgagggcgg tgaagacgag gattttggtg gaattggtaa
    23281 agctcctgta agaaaatctg ctaaaagaaa tttgaacaca cctttagcaa atccatcatc
    23341 taaagagcca tcaattgaaa agaagagtgc gtttgctatt aagcttgaaa aaaataaaga
    23401 agaaaaagct aatcagggag ctaagtcact agaaaaactt cagcaagaaa aattagaaaa
    23461 actagaaaaa gcattatcat catcaacttc tgaaactcaa tcttcaaagg gtatagataa
    23521 tggacaatca attacaacta aaaacaagcc taaaaatagt aattcaaatc tctcggtaaa
    23581 tgttgttatc acaactgata aatctgaaaa aaaacaagtg aacggaacta cttcacccat
    23641 catgcaaaaa atgataaagg caaaaaaaca ggtgcagaaa tgattatcat tagttccttt
    23701 ttgagatggc taacaccata taaatataca gatgaaatag ttgagaagcg agcgaagaaa
    23761 aaagttaaaa aaacgatgtt aactcctaaa gaaatgctag atcgtggaat attgggagtg
    23821 aaggttttag aagtaaacca accgtttgaa ttggataatg cgagagatag gttgcttaga
    23881 gctgaagttg atgtcgcata cttttatcat gataaaagca agcatcaaat agctatcaaa
    23941 ttcaatccaa aatcggcatg gcgataccaa gcaagagatg acctaaagcg taggaagctg
    24001 aaaccgatgc tctatcctga aggaaaagag gcatttgata gaacccatat tgttcccatt
    24061 ggatttcatg gtacagagtc agataaccgc ctattggtag ggtggaacag ctcacaaaat
    24121 agagttcaga tcaataaatt tgagaagaag gtaagaaaat ttaacgatcg gaaaataata
    24181 ctttggtttg ccaatattca gaagcaacca gatgggacag caaagtggga agcggtcata
    24241 tttgatgaag atggcaaaaa agtaatgtct gaagtatggc atgataaagc taaatttgtt
    24301 tggcagtaga ggtgaaaatt gtgttaacta acctaaaact atatagagag ggatttaacg
    24361 aaaaagtaag tatcggtgaa tctattgaat atttaggaaa gaagtacatt attattcgta
    24421 ttcttgaaat taaaaaactt tattttagta aatataataa tttagcagtt agagttctcg
    24481 tccaaaaggt tggaaatcca agtgactata gaagtgatta ccataactat aaaacagaat
    24541 catctgtaat taagcgttat aatcaatcaa aaaatgatga gagaatattc aaagtaggag
    24601 aaattattat ttctaagtat gggatagcat acgaaatagt ttctattaaa aaattttatt
    24661 atgaatttgt tgatttggtt gtagaatatt ctgttcgatt gatcgttccg tggagtaaag
    24721 aagaaattaa tgaggcatta aaacatgaac gaaaaagtac attcaaagtt ttagaaggtg
    24781 aaaaaaagtg aaaacagacc ttactaggca agctgagaag tgcttatggc atgaaacaaa
    24841 tcgcatgggt gtatttggtt gtttcgaagt cactatcggt tgggatggca gtgaaagagt
    24901 agattatata acttattcag tagataacac agttcgatgt tatgagatca aagttacttt
    24961 agctgattta aaaagcaagg cgaaacagac ttttatcggt gattataact atttagttgt
    25021 tactagtgat ttgtgggaga aaatgcaaca agacgataaa cttatgcgga aatattacta
    25081 tcaaggcgta tacgtgttca atgaaaaata tcctagcata ataagtgtta agaaagcaag
    25141 gaaacaaaaa gtatcgctag gtacaagatc aactgtatta gaaagcatgg ttcgttctat
    25201 gaatcgtgaa gttaagaaac tctataaagt aaaaccattt tgggagatag cagaggtgga
    25261 aaaaggatga ttcaaacatt tgcaaaaatg agtagaagtg cttggattga aaagctggaa
    25321 aatgatttga gtgttatgcg attagctcca aagaaattca aacgagagat tgcagaaaaa
    25381 gaagcaattt tgtcggtatt gaatgcggta gaactggacg aaccgaaaaa gtcagttgat
    25441 gtacctgaaa atgtagcaga gttactagat tattatcgta attcgtcaga tgtcgatttg
    25501 ttagctctga tattaacatt tcacgattgg tattactgtc aaaatgaaga tactcagcat
    25561 gataaacatg ataaagcagt tgactggtta atagagaatc cagaaaagtt catgcgtgct
    25621 tggctagatg gctatgatag caagaaaaag ccactggaaa gtaatgagga aacgtattta
    25681 acggcagaac aatatgacta tgctagaaaa acaggagatt gggaatctgg taagactttc
    25741 aatcacagaa aagatcaacc aaaaattagt ttttcagata tagccgcaat tatcgaaggt
    25801 atctactatg gacaagccga tgaagagatc ttttctatac aagatttatt agaatttttg
    25861 aacaaaaata aggatagctt tgtagaacta actgattatg aggaggatac agcaaatgga
    25921 aaattatgat ccaaatatgc gatggggtac acatacgctt aaagtttctt ttcaacgttg
    25981 ggattacaag ggatttgtga cttttagaaa agctggaaat tgtaaaggat tagatgtctt
    26041 agcgttggat gaagactatc tatatgacca tccattgacg gataatccaa tcggatttgg
    26101 attgcttcct gaagatgatg agggcaatga atggttcaaa atgattctta caaacgataa
    26161 aggtgatcaa ttatttgtag aagatacttg gtcatatttg agtgaatata ttgttagcat
    26221 taaaatcatt gattttgtag cagataaaga aaaagagatt ggagaaggta aatctaatta
    26281 ctagctaatg aatagcaact ttggaggata agtcaaaaga ataaaaaaag aatactgtgt
    26341 gtcattgcag ggatgtatct ttgcatgata gtatctgcgg ttaccattta ttggttagga
    26401 aatcagcaaa acagtttaat tgttgatctg ggggagttaa aggttggatt acgaatgact
    26461 agatggcttt tattagctca aacagttggt ttagccatat tgacttggat atgtgtggaa
    26521 aaaataacat ctaataataa tcaagagatt gttgaatcaa caaacctaag aataagaata
    26581 actgaacaag caattgaaaa gcaaaaagac aaagaaaaaa aggaaataat gtcaatttat
    26641 ttatctagtg gagcatcatt ttttcatgaa atagattcga tgaaactgag tagtcaagaa
    26701 atcattgata gatggaaaaa catgcctcta ggtggtgagt taatcgttga agatagtgat
    26761 ggttggtcag cttataagaa ggacgagatt gtagctatca cgattttgtt tgaatagtaa
    26821 tatattacca ctagataatc tgaatatgtt gatctgctag tgacgtacca aatgcgttta
    26881 atagaacctt ggtcacaggc agaaattgat aaagcagtaa aattgaaccg attatcaaaa
    26941 tttaacgtat taggaaatgc cttttaaaaa tcgcaataca caaacagaaa aataccaaaa
    27001 aattagcaat gaacgtaggt aagaacatga caataaataa taataaccct aacaaatata
    27061 atgctcttgt ccaatgctat atcgaaaaca aaattacaat gcaagaagtc gctgatgaat
    27121 taggcaaatc aaaaagccta gtatcacacg actttaagca caatgttttt ccgaacccga
    27181 ttgagctgag gaaaaaattt caaaagagag tttatcaaga aatagggaag tggattgatg
    27241 aagggaatgc aattgaccct ttaattgaaa aatatccttt tttaaaaagt aaaaataaaa
    27301 ttcaaaggtt aattcgtcaa ggcgatattc aagctaaaga acctgttttg acaaccaatg
    27361 atttggctaa aataaatctt cataaacaga ttattgaaga ctttgaaaaa aatctttcaa
    27421 caactgaaat tttaaaaaaa tacaatattc cgagaggaaa gctaacatcg actcttttta
    27481 gagaatatgg agaatcggca gttactaaag caaaggaagt agcgaatgag agaaaaaaac
    27541 aaaaatagta gtcgatacct agtagcgact gtagtttttg tttgtgtatt gattttcctg
    27601 tttgattttt tgcagattgg aaaaagcata attggaaata cattgagtgc aaaggaaatg
    27661 gttgctttag gtggtttgta tgaaggacat ttgttcccga atatattcat ttctatcgtt
    27721 tctcatatta acttgtacca tttaatttct aatttattgt tcttagtagt tcttgaagaa
    27781 gcactgaatg atcaatatga gtggttaaca ctattgattg ttttaatcat atcaggactt
    27841 ctaggaagct tagttccttt tctgttacaa agcaacatcg taagtatggg gcttagtgga
    27901 ggtatttttg gatggattgc tttatttgcg attgcataca tccgctttga tagtaaggaa
    27961 catttattac taagtgtttt tatattattg ataatcggca ttttactggt atctactttt
    28021 gaatcaggcg agacaaacgt cttgtcacat gtaatcggat ttgtctgtgg gatgatcttg
    28081 ggatttattc taaagaacat ttcattgatc aagcaaaaga attaaaaggg agtgctaaaa
    28141 atgccaaaaa aaaaagaaaa gaaaattcca gtgtttttgt ctgtctcaga aatgttatca
    28201 cttcaaatac tgttaagaaa aaagattgaa acaatagacg aagaagttag aaataaaagg
    28261 gctatgtacg aacaacgtga agcgctaaga gaacgaatcc actattttga tgatatacac
    28321 actaaaatct atgaagcacg tacaaataac gaagacgaaa gcaaattagg attagctcaa
    28381 caacgaatat ttgaatggct aaatacgaat actgagcaag aaccgttaag agcaattagt
    28441 aatctttttg aatttggaac gaagcattgg tttaacgcag tagaaaagga tgctgactta
    28501 caagaagcct ttgacacgct aagtaaggat agcaaatcca tgcgagattt attgattact
    28561 tatctaaatg agcatgagtt tgaacaaacg aaaaccaaaa agaaaaataa agaatagaaa
    28621 gttgtgatga atcatgctat taaccctgtt aggttattta tgtttaattc ttgctttttt
    28681 tggggcaatc gctataatta ttggtcaatt tcaaacaaat aatccgatta tgcgtaacat
    28741 aggcattgga attgtgattg taacctttat tgtgtttaat gccactaaga tttttggaag
    28801 taacgataaa caatttggta tatcgggagt taataatagt tatgctccag attctttggg
    28861 aagggtagta atagatggaa catttgaaaa tgtaacgagt tataaaaatg tttcgctgac
    28921 agtgaaggaa gatggcaaga aagcatcaga cgacgttctc gctactatta agccgtctca
    28981 atcacaattt gaagtgtttt atacagttaa agagaatagc cctgtacaca agctgacatt
    29041 ggtttttaac gttgatggag aagaatacta taaagatgta aaaataaata aaacatcaac
    29101 gaaaaagatt atcgaatcag cgaaaaaaga agtagctaca gaagaaagta caactgaatc
    29161 aagttctact gtcgaagcgg aactaccaga taaaattgat tttgatcctg aaaaagtcaa
    29221 atcgtttgaa tctgtttctg gttctatggc tgatcggttt aacactgaag tcttcaaagc
    29281 tatcaaaact gttaaattgg atactgaaaa gcaaatgatt aatgttacct tattcaattc
    29341 agttgaatct atggatcagg ataaaaaagt tacggttgca agagaactag gaaaaagagt
    29401 aattggtttg tatcgtggta ctgctaaacc agattcagac ggtgcagatt tgccagttca
    29461 tgtagtttat gaagacggta aagtttttgg agattctaca gcaaacgaag cattaacgat
    29521 taaattggct aaatagccag aaagttataa taaacaaatg gtgtcacacc aataatatat
    29581 aggtgtgaca ccttggagat gatagctgat ggtagtaaat aaagaaaaag ttagaaagta
    29641 tgtgacttta aaaatagctt ctgttaaata tttagaaaag cgagctaaag aagaagaaag
    29701 agcaagaggt aagaaagtaa cagtttcaga gttgttagaa gaattgatcg agaatgatca
    29761 aacaataaga cgtgcattta ctaataggtc tatcagataa caatataatt aataaaaaag
    29821 aaagagtgat ttggtcttag actaaatcac tctttttata taaagtttct ttacactcat
    29881 tccgaataat gttaaaattc tttatatgat atatagttcc ttgtcagagg aagagataac
    29941 cagcaatatt ggagggataa taagtggaaa acaataatga tattagacat tttagcattg
    30001 gggaaagagt tacaacagta agtgaattat caaaggatta tagagataaa gagggaacga
    30061 ttatttctct taataagaat cgaagaggcg tttggcaata tgttgttgat ttaggtgatg
    30121 gcgttacagt tgagtgtaaa gatagtcaac tgatcagaaa taatggcagt cattccattg
    30181 aaaaagcaat tcttgtatac ccaaaagtat caagtaagat catgaatgac tggttgcttg
    30241 gaaaatttgg tgagtacaat aaaaaagaaa tcgcagaagt gatcggcaaa tcttatccaa
    30301 cagtattagg gtatctaaaa aagataacga gtaaagaagc aaaaattgag gatgttttaa
    30361 cagtaagaga gcttcaatca ttaatcgagt atcggaataa acaaataaca aatcctgagt
    30421 accttagaaa agaagatggt ctattgaaac aattggaaca agtaaataaa atccggaaga
    30481 tgcaaaaggt agctccttta agccttgata cctttaaaaa aatctatttg aaagagatca
    30541 aaaaagacta atgtggactg taactttacc gttccaacct gtatctgcac caagaccaag
    30601 tgcaagaagg aatgctgctg atgaaactaa tgacgtagca gagaaaagga tttcaaccta
    30661 ctatgataaa aagtatcttg attatttgaa aaaaataaag agctttatcg aagatcaaga
    30721 tttattagat gatgaatttt actcgattgt aaatgatcct atgggcgcaa ttatggaagt
    30781 ggacttttac taccagatac caaaaagcta taaaaaagtc tataacatta tgaagacaac
    30841 agcgccagat ttagataatt tagtaaaaag tgctatggat ggtattttta acatctcagc
    30901 tatacgagat agccatatca caggcttaat tgcttttaaa tataatgtgg cgaacgaagg
    30961 aaaaacagtt gtgaggatca aacgatattc agaatttgag tgggatactt ctgaaggatt
    31021 gaacggagat atatgggaag ctacatttga ttttaaacca gtagcaacgc caagaccaaa
    31081 gtctaaattt agaggaaatg ggattatcac ttactatcca aaagagtaca acgactattt
    31141 ggaaaatatc aagaatacgc ttaaagagaa agatttggta aatgatcaac tgaaaaaggt
    31201 aatgaatgct cctatggggg ttatagctca gatagattat ttttaccaag tgagaaaaga
    31261 taagaaaaag cttgattcac tgatgcgtac ttcacagccg gatattgata atttagtcaa
    31321 aggaacattg gacggaattt ttaacaagga aataggcatc aaagatagca gggttgtagg
    31381 gttaattgct tttaaataca atacttttga aaagtcgaaa acaaacgtga gactacagga
    31441 gatgggctga tgaataataa tgaaaaaatt cttcatgtac ttgatagctt tgaaataatt
    31501 caagaagaac taaaaaaata tcgggatgtt ttggaacaaa gatatgattt tgtcaatcag
    31561 caaaagagta atcacatgga tttcattctc aatatgaatg acttgaagaa gaaattagtt
    31621 gaacgaaaag aacaagaaaa gttgatcaaa gcttactttg aactcggtga aaaagaagtt
    31681 aaaaatgcaa tggaactgaa tgaagagcgc agggttttag atcagctgtt agaacaatta
    31741 ttggtgatgt ttcaaaaagg acgaatagat gaggatttaa ttgaagaagg attgagaaaa
    31801 tatccttcaa actcaggtat cggaatagtg ctgaaagcga ttgatgaaga agaaattgaa
    31861 gctttcatac cgccagagga ttttgaatcg gcaatggaat atataaaata ttatagccaa
    31921 gggatcactg cttttagaga gtttgatcct gaagatgtta ttcatgactt gaacaaccta
    31981 aaagaatggt gtgaaagtta tggagtcgat gattccggat tggactactt gatcagtatt
    32041 atggaaatcg aagaagagat gccggataag cctgatccaa cagatatttt agaattgatc
    32101 catgaagcta gaaatccaat tgcctatatt tctagaggat atactgtatt ggaatactat
    32161 aagccttata tatcagcgat gaaccattta agacgtgtat taagggaaaa acgtgaatac
    32221 cgctcagtgt tgaatgcaac gaatagatta gaaaaagctg tttcagagct agatgcttat
    32281 tatcgtgaac attacctgca agcaggtggt atgccaagaa atacgaaagc aaatatcagt
    32341 aggtatataa aaaaagctga gtaggcggag ttagcccata aaatggcaaa aggtaacctg
    32401 aaaggggcat tttgaatgaa aagggatgtt tttgaattgt acgtattaga acgtctagaa
    32461 gacggcatga tggcacaaga gataattgat gaaattgatg atatgatcag cagatttggt
    32521 caattggagt tgaatgatgg aaacaaaaat tcggtagtgc taaaacaatt ttggttgaac
    32581 aatttttcgt ataaggattt tatcaaagaa aaggcaaata cattgtactg actaaattca
    32641 cgaggagcag tgatgagagc ttattgctaa gagcagttag gctctttttt gtttgtgtaa
    32701 aattatttgt tttataatac acatttatac acaaaaaaat attgacagat agtacacaaa
    32761 tgtggtagga tatatttg
//
LOCUS       DASJST010000016        31896 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJST010000016 DASJST010000000
VERSION     DASJST010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.016"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..601)
                     /locus_tag="U4B05_000560"
     CDS             complement(26..601)
                     /locus_tag="U4B05_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2049719.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="U4B05_000561"
     CDS             complement(606..1580)
                     /locus_tag="U4B05_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HEN2049720.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="U4B05_000562"
     CDS             1863..2831
                     /locus_tag="U4B05_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2049721.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="U4B05_000563"
     CDS             2928..3221
                     /locus_tag="U4B05_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2049722.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="U4B05_000564"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="U4B05_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN2049723.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="U4B05_000565"
     CDS             3678..4433
                     /locus_tag="U4B05_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2049724.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="U4B05_000566"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="U4B05_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN2049725.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="U4B05_000567"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="U4B05_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN2049726.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="U4B05_000568"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="U4B05_000568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN2049727.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="U4B05_000569"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="U4B05_000569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN2049728.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="U4B05_000570"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="U4B05_000570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN2049729.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="U4B05_000571"
     CDS             9170..9712
                     /locus_tag="U4B05_000571"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN2049730.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="U4B05_000572"
     CDS             9735..11291
                     /locus_tag="U4B05_000572"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN2049731.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="U4B05_000573"
     CDS             11305..12207
                     /locus_tag="U4B05_000573"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN2049732.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="U4B05_000574"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="U4B05_000574"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN2049733.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="U4B05_000575"
     CDS             13650..14072
                     /locus_tag="U4B05_000575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN2049734.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="U4B05_000576"
     CDS             14541..14771
                     /locus_tag="U4B05_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN2049735.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="U4B05_000577"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="U4B05_000577"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2049736.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="U4B05_000578"
     CDS             16182..16358
                     /locus_tag="U4B05_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2049737.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="U4B05_000579"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="U4B05_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN2049738.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="U4B05_000580"
     CDS             16853..17320
                     /locus_tag="U4B05_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049739.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="U4B05_000581"
     CDS             17405..18289
                     /locus_tag="U4B05_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2049740.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="U4B05_000582"
     CDS             18403..18639
                     /locus_tag="U4B05_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN2049741.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="U4B05_000583"
     tRNA            18727..18807
                     /locus_tag="U4B05_000583"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="U4B05_000584"
     tRNA            18814..18885
                     /locus_tag="U4B05_000584"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="U4B05_000585"
     CDS             19011..19181
                     /locus_tag="U4B05_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049742.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="U4B05_000586"
     CDS             19401..20108
                     /locus_tag="U4B05_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2049743.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="U4B05_000587"
     CDS             complement(20167..20811)
                     /locus_tag="U4B05_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN2049744.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="U4B05_000588"
     CDS             20938..21225
                     /locus_tag="U4B05_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049745.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="U4B05_000589"
     CDS             21333..21653
                     /locus_tag="U4B05_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2049746.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="U4B05_000590"
     CDS             21971..23047
                     /locus_tag="U4B05_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2049747.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23763
                     /locus_tag="U4B05_000591"
     CDS             23044..23763
                     /locus_tag="U4B05_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN2049748.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="U4B05_000592"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="U4B05_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN2049749.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="U4B05_000593"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="U4B05_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN2049750.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="U4B05_000594"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="U4B05_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN2049751.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="U4B05_000595"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="U4B05_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN2049752.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29200..30093
                     /locus_tag="U4B05_000596"
     CDS             29200..30093
                     /locus_tag="U4B05_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049753.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="U4B05_000597"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="U4B05_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2049754.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10607 a         5565 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DASJST010000017        31501 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJST010000017 DASJST010000000
VERSION     DASJST010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.017"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..1157
                     /locus_tag="U4B05_000598"
     CDS             288..1157
                     /locus_tag="U4B05_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN2049755.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1366..2391
                     /locus_tag="U4B05_000599"
     CDS             1366..2391
                     /locus_tag="U4B05_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2049756.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2430..3380
                     /gene="manA"
                     /locus_tag="U4B05_000600"
     CDS             2430..3380
                     /gene="manA"
                     /locus_tag="U4B05_000600"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN2049757.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3474..5177
                     /locus_tag="U4B05_000601"
     CDS             3474..5177
                     /locus_tag="U4B05_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2049758.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5179..7212
                     /locus_tag="U4B05_000602"
     CDS             5179..7212
                     /locus_tag="U4B05_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2049759.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7223..7663
                     /locus_tag="U4B05_000603"
     CDS             7223..7663
                     /locus_tag="U4B05_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2049760.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7863..8990)
                     /locus_tag="U4B05_000604"
     CDS             complement(7863..8990)
                     /locus_tag="U4B05_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049761.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8993..9670)
                     /locus_tag="U4B05_000605"
     CDS             complement(8993..9670)
                     /locus_tag="U4B05_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049762.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9934..11700
                     /locus_tag="U4B05_000606"
     CDS             9934..11700
                     /locus_tag="U4B05_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN2049763.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11715..12152
                     /locus_tag="U4B05_000607"
     CDS             11715..12152
                     /locus_tag="U4B05_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2049764.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12165..13316
                     /locus_tag="U4B05_000608"
     CDS             12165..13316
                     /locus_tag="U4B05_000608"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN2049765.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13378..14076)
                     /locus_tag="U4B05_000609"
     CDS             complement(13378..14076)
                     /locus_tag="U4B05_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN2049766.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14069..15421)
                     /locus_tag="U4B05_000610"
     CDS             complement(14069..15421)
                     /locus_tag="U4B05_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN2049767.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15644..17044)
                     /locus_tag="U4B05_000611"
     CDS             complement(15644..17044)
                     /locus_tag="U4B05_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN2049768.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17205..17942
                     /locus_tag="U4B05_000612"
     CDS             17205..17942
                     /locus_tag="U4B05_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN2049769.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18067..18546
                     /locus_tag="U4B05_000613"
     CDS             18067..18546
                     /locus_tag="U4B05_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN2049770.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18543..19310
                     /locus_tag="U4B05_000614"
     CDS             18543..19310
                     /locus_tag="U4B05_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049771.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19540..20109
                     /gene="lepB"
                     /locus_tag="U4B05_000615"
     CDS             19540..20109
                     /gene="lepB"
                     /locus_tag="U4B05_000615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2049772.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20213..20815)
                     /locus_tag="U4B05_000616"
     CDS             complement(20213..20815)
                     /locus_tag="U4B05_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2049773.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20921..21390
                     /locus_tag="U4B05_000617"
                     /pseudo
     CDS             20921..21390
                     /locus_tag="U4B05_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21496..22080
                     /locus_tag="U4B05_000618"
     CDS             21496..22080
                     /locus_tag="U4B05_000618"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN2049774.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22083..23156
                     /gene="prfA"
                     /locus_tag="U4B05_000619"
     CDS             22083..23156
                     /gene="prfA"
                     /locus_tag="U4B05_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN2049775.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23149..23988
                     /gene="prmC"
                     /locus_tag="U4B05_000620"
     CDS             23149..23988
                     /gene="prmC"
                     /locus_tag="U4B05_000620"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN2049776.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23981..24160
                     /locus_tag="U4B05_000621"
     CDS             23981..24160
                     /locus_tag="U4B05_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049777.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24182..25207
                     /locus_tag="U4B05_000622"
     CDS             24182..25207
                     /locus_tag="U4B05_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN2049778.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25398..26642
                     /locus_tag="U4B05_000623"
     CDS             25398..26642
                     /locus_tag="U4B05_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN2049779.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26795..27742
                     /locus_tag="U4B05_000624"
     CDS             26795..27742
                     /locus_tag="U4B05_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2049780.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27876..28505
                     /gene="upp"
                     /locus_tag="U4B05_000625"
     CDS             27876..28505
                     /gene="upp"
                     /locus_tag="U4B05_000625"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN2049781.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28644..28886)
                     /locus_tag="U4B05_000626"
     CDS             complement(28644..28886)
                     /locus_tag="U4B05_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049782.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29007..29079
                     /locus_tag="U4B05_000627"
     tRNA            29007..29079
                     /locus_tag="U4B05_000627"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29040..29042,aa:Thr,seq:cgt)
     gene            complement(29198..29836)
                     /locus_tag="U4B05_000628"
     CDS             complement(29198..29836)
                     /locus_tag="U4B05_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2049783.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29959..30591)
                     /locus_tag="U4B05_000629"
     CDS             complement(29959..30591)
                     /locus_tag="U4B05_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2049784.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30942..31241
                     /locus_tag="U4B05_000630"
     CDS             30942..31241
                     /locus_tag="U4B05_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2049785.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10751 a         5490 c         6329 g         8931 t
ORIGIN      
        1 ctttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga
      121 acagcttttt cacaacctct ttttataaga gtttttactc attaatttac aaaaaacaag
      181 caaaatgcga attttctcac atttttttta acaaaatcaa agttattgct tgaaagaaga
      241 agaatccatc gttaaaataa agcttgacgt agaggggagc aagaaatatg aaagattttc
      301 aattgggggc tgccacctat ttaaaaagtg tagccattcg ggtgaaagac cgagataaaa
      361 tgattgattt ttacaagaac gttgttggct ttgatttgaa aagagaagaa aacgaattag
      421 ccattttagg aacagaagaa gctggaagtg aaatgttgct gctggaagaa agtcctagag
      481 cgaatgatca catgggggtg atcaaaaagc tgaaccgttt ttcattgatt atccctagca
      541 cagaagaaat ggcagatatc ctttgtcgta tccgtaaaat gaattatcca atcaaagaag
      601 ctttagaaga tcatggacgt atggggattt tgctcgtaga cccagaagag aatgaattgg
      661 aaatttacca tgaagagaat aagaacgacc aaagagaaat tccagaaaca atggatcaag
      721 aagcattatt aagaaaatct gaagaaaaat tcagtaaact atcaacaggt tcttattttg
      781 aaaaaatcca tttaaatgtt actaacttaa caaaagaacg ccaatttctt aatcatgtac
      841 ttggtttgaa ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagatttcc
      901 atgttggttt gacagaagct agcggaggcg caattgattt gcctacagat gaagtgattg
      961 gacttgattt tctgaaattc atgatcgata ctgaaagcat ggaaggactg attgctcact
     1021 tgcagcaact atcagttgat ttcttcttag acaaaaagca cacagtactc acaatctatg
     1081 atgctatagg aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca
     1141 ttcgatgcga cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg
     1201 aacggtcata ctatcgctta ttctagagat agaaagagaa aatagtatac cgggggaaac
     1261 tgcgcggttt ttcgcgcagt ttttttgtgt aaaaaaggtt gattcctcat agattaagct
     1321 ttaagcttgg actgtaagaa tctgatagaa aagcaggaga aaaagatgta tccacacacg
     1381 aaacagctta ttttgaaaaa gatggaagaa ggattttatc caggtgtagc agtcgccttc
     1441 ataaaagaaa atcaagtaga aacatttaca gccggctttg cccaagtcgt tcctgaaaag
     1501 cagctgatga cagaagatct gttgtttgat gcagcctcac tgacgaaagt cgttgcaacc
     1561 acttcattag ttttacgtct attagaagat ggaaagatag aactcgatca accgcttcat
     1621 aaatatcttc ctgaatttga aaatcagaca atcactttgc gtcatttact gacacatact
     1681 tctgatatca aaacatggat accaaacaga gataagctat ctcaggaaga attgatcgcc
     1741 gcttatttga agctagaacc aggtgagcaa ctaggaaaag aagtaaaata tacagatgcc
     1801 ggtacgattc ttcttgggtt gatgctagag gaaatttacc aagaaactgc agtggaaatc
     1861 tttcaagaac aagtattgga accattagga atgatgaaca gcttatttct tccttttcct
     1921 tccaaaaaaa tcgttcccac agaacaactg ccttcgggtg aggttttgcg tggacaaact
     1981 catgatccga aagcaagagt tttggctgaa catgcaggta acgcgggtct ctttattgat
     2041 ttgaaagaca gcattacttt tgtccaaatg tatttgaaca agggaaaaat ccgtaatggt
     2101 cgttttttag aagaagagac gattgattct ttactgcaag atcaaacacc gaaaaaaaat
     2161 ggctatcgtt ctttaggatg ggacttaaaa aaggatgtag aagacggtca tgcgattctt
     2221 tttcatactg gttatacagg tacgtttttc ttgattgata taaaaacaca agaagcgttg
     2281 atatttttgt caaatcgcgt acatcctgtc gatcatcgtg cggagtatct catatgtcga
     2341 gatgaaattg ttgaaaactt tttgaaagag aaagcggtat taccgaaata atgctataat
     2401 agctatgaaa aatgagagga gtttgaatta tgcaggaacc tatgttttta aaaccagttt
     2461 ttcaagaaaa aatttgggga ggaagtcgtt tacgttcagt ctttggtttt gatatcccta
     2521 acgataaaat cggagaagat tgggcgatca gtgcgcatcc tcatggtgtc agtgtagtag
     2581 aaaatggcga attcaaaggg aaaagattag atgaactatg gtcagaacac aaggaattgt
     2641 ttggccatcc ttcagaacca gtgtttccat tattgatcaa gatcttagac gcagaagatg
     2701 atctatctgt acaagtacat ccagatgatg cctatgggat gaaacatgaa ggtgaattag
     2761 gaaaaactga atgctggtat atcatcgatg ctgaacctgg tgcagaaatc atctatggtc
     2821 atcatgcaaa gacaagagaa gaactagctg agatgatcaa agatgggcgc tgggatgacc
     2881 ttttgaagaa agtaccagtt aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc
     2941 atgctattgg taaaggaatc atgattttag aaacacagca aagttctgac acgacttacc
     3001 gcgtgtatga ctacgacaga aaagatgcgc aaggacaaac aagagagctg catatccaac
     3061 aatcgatcga tgtaacaact gtccctgcga aaacaccaga attacaaatc aaagaagtcc
     3121 gaaaagggaa ttcatcgatc gtcacttatt tagaaactga gtttttcaat gtatatgagt
     3181 gggatattaa aggaatcaca tctttcaaaa aacaagcccc ttatacattg atgacggtaa
     3241 tcgaaggagc aggagacctt gtcgttgacg gaaaaacata tccattagaa aaaggaacaa
     3301 gctgtattat tccaaatggc gtttctgagt gggcggtcca aggtgaacta gcaatcattg
     3361 cgtctgaacc aggcaaatag ttcccagata gctgaaagca gcagacggtt tttcgtttgc
     3421 tgctttttct acataaaatc cgtccatcaa taaatagaag tctggtgaaa aagatggaat
     3481 acaaatggaa agcaaacaac agcagtcgcg tgtctatgaa acaactgaga aaagctggac
     3541 atcacttaaa tgaaatgatc atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt
     3601 cattagcaac aaaaggtgtg tctggcgatt tgcacaccac attagaagag gtatgccatt
     3661 ggatgatcca gctgctttta cccatcttga tcagttattc aggcgggaaa tcggtagggg
     3721 gacaaaaagg ggcagtcgcc ggtgcaattg cttctttagg gttgatcgct cgttccaatg
     3781 ctccacaaat cgtaggagcg atgattatcg ggccgcttgc cggcatcgta tatgaacaat
     3841 ttgtttctag attccatgtg aaattcaagg ctggatatga aatgcttgtc agcaacctac
     3901 tagtaggact gactggtagc gttatttgta taattggaat cgtttttatt gaaccagttt
     3961 taaatagtgt tcacttgttg ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt
     4021 taccacttgt cagtctgatc ttagaacctc taaaagtact atttttcaat aatgcaatca
     4081 atcatggtgt attgacacct ttaggaatcg aattctcaca aacagtcggg tactcttcct
     4141 tatttttgat ggaggcaaat cctggacctg gtttagggat cttactttct atcctttgtt
     4201 ttgcagaaaa acaggaaaaa gtaaatgcga gtggagcatt gatgatccat gcgattggcg
     4261 ggattcacga aatctatttt ccatttgtct tgctgcgacc gtatctattt ttagctgtaa
     4321 tggctggtgg ggcaagcggc acgttgatat ttcaatggtt agatacggga ttagctgcac
     4381 ctgtatcgcc tgggtcacta ctcgtgattt ttgccaatgc gccttatcca caactagctg
     4441 gggtaagtat agggatagca atcagtacat ttgtgacctt catttgtgca gcgatactat
     4501 tgaaattacc aaataaagaa aaagagaaca aaacggtcaa agaaagggga gaaatcttgg
     4561 aaccatctat cattcattcc gtcatttttg cttgcgactc aggtatggga tcaagtgcta
     4621 tgggagccgc cctgctgaaa aagaaattag cagcagaaaa aatcgatctt tctgtcggat
     4681 acagctctat ctatgaattg aataaccaag taaatcagct ggttatcgtg caacatgaac
     4741 tagcgggtct tgcaagagaa aaagtacctc aaagtcaaat ctatgtactg gatcactttt
     4801 tggaaacaga tatcttgctg tcacgaataa aagaagcgat ccaatttcca aagcgggaag
     4861 aaaaaaaaga gatagcagat atcacagaac aaaaaacgtc gcctgctaat attgtatttt
     4921 tgtataaaaa caacagacgc ggctcacaaa ctatgggcat gacgatttta agtcaattag
     4981 caaaaaaagc gaaaaaacag tttgttatct caaaagaatc aatagaaaaa atcaaaatcc
     5041 aagaagaaac gatttatatc gcacaaagag aactagctgt aactgaacag ctagaagaaa
     5101 agatccctcg gctagttcta gttgaacatt gggtagctac gaatgaatac gaaaaactcg
     5161 tgaagggaga aatataagat gtatttatcg cccagagaaa aacaattatt agcagaattt
     5221 ctcagtagcc cgtcacctgt atctatccaa aaaatgatga atttattgaa ggtcagtaaa
     5281 cggactattt accgcgaatt agataatctt gaagtctcat tgaaatcggt aaatgcttct
     5341 ttgaaaaaag cagctagagg ctcatttgtc attgaggcat cagaagaagt attagagcaa
     5401 ttaaaagaaa atacagatgg gaaaagtaat gacttatcca ctgctaaacg acaacacgcg
     5461 attttagccg agttgttact gacgacagag ccgttgagtc ttgctcattt catggaatca
     5521 tatcgcatca gtaataccac tttttatgca gatatcaaac agttggaaga aagtattcgc
     5581 caactgcctt tggaaatcat tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac
     5641 cgccggcttc tgacagcgaa tgtattagaa ttagaaatca atgaatatga actttttcat
     5701 agtatttctt tcgatgcatc actgaattat ttttttcgat tcgttgatcc gcaacatcta
     5761 tctcttgcac gcaaagtaat cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat
     5821 agaaagcttg aacatttggt tttgatgctg acattgacga tggagcgtgt gacgaaaaat
     5881 catttactga caaatgagac atataatagt ctggcaaaca aagaattact acagacagct
     5941 aaacgattgt tttcacggat cgcttctgag acaaaacaac tttatccagt aaatgagatc
     6001 gtcttttttg caggattatt aaatgacttc acgaactcat ttgatcagaa tttctttgaa
     6061 gagacgttcg atacacagct tgcttattta gtaaaacaac tgattcaaga agtaagtgaa
     6121 gagacgaatg tccattttta tgaagatgaa actttgtaca aaatgctgtt gactcattta
     6181 tctggtgtgt tttcaagagc cgttttacaa gaagagacac tgaccaatcc gattttagaa
     6241 aaaatcatgg ttcaatacga ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt
     6301 tttcaagaaa aaaaattatc cgaagaagag atcgcttata tggtccttca ttttgcgaat
     6361 tcattggaac gaagtccgaa agaaagcaat atcagtattg caggattttc gccaagtgga
     6421 ttagcttcta ctagcatgtt ggaactacga ttgaaaaggt attttccctt catccaacaa
     6481 attcatttct atcggattgc tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt
     6541 gttatttcta catctatttt accaggatac ggtggaaaat atattttagt ctccccgcta
     6601 ctgctagaag atgaagtcaa gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa
     6661 aggcaaaaaa ttacgtataa cagcaataac gagtggatga tagaagatac ttatgaggaa
     6721 acaatgcatt tcatggatca aatcaatcaa ttactcggtc gtttttttat ccaagaactg
     6781 gataatcctg agacgatttc cgaaacgatt cgcagcttat ttccttattt tcctgaagga
     6841 ctagtaacgg atgaagaggt ggttcatcgc cggctgctta agcgatatga acaagcacct
     6901 atcggtattc ctcatacaaa tatggggtta ttccatacat ccagctcatt gatttctaaa
     6961 ccgatctttt gtattttcaa tttgaaacat ccgttgacca ttgaagggat ggatagacag
     7021 ccagtcgatt tgtcccgtat gttagttatg ctggcgcctt cgccaatcca agaaaaagac
     7081 gggattcttt taggaaagat cagtggagca atcatcatga atgacttgaa caccgagatc
     7141 ttccgctcag gaaataaaga aatcgtttat cagttgttag cgaaaatcct gattgaagaa
     7201 ataaagaaat gaaggtgaaa agatgataca gctaccagca gaaaaaatat tgctcgatca
     7261 atcctatcaa aattggaaag aagcgttgac cgagatcggc cagtggatga cccaacaagg
     7321 gggaatcaaa ccggcatata tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta
     7381 tatcgggaat ttcgtagcct tgccacatgg gactgacaaa gatgaagaag tagtagaaga
     7441 aggaatctat ttagtccaag tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa
     7501 aatcgcgaca ctcttatttg tagtggcttt aaaaaaagaa catcagttag cggtgctcca
     7561 agatttagcc tttttctgtt cagacatcga ccaagtaatg gcgctcagtg atgcggaaac
     7621 cattgaagaa gtccaaatga tttttgaaca tggacatata taaccaatgt tcacagtaaa
     7681 aaaagctgcc ttgcatcaag taataacttc aaccaagata aaaataagag gagctatttt
     7741 tgttgggagg ttcttattac tgcggatgca agcttttgaa aactatttat tctagaaaac
     7801 cgtttatttg aaatagggga cggtgacaag gtttttgtca ccgcccctat tatttttgtg
     7861 ttttaatagc tcttttttat atagatagtg cccataccta tgaataataa aacgatactc
     7921 catataccaa gtgtcaagat accattcatg aaagtaacct gttctgaagc tagtggcgag
     7981 aaaatatctc ctcctatgat cacattttga aaatcaacat aagaagttgg caagtaatga
     8041 ctgattgatt ctatccatcc aatcgaagaa aacaagcgat actctgctaa gaaaagacaa
     8101 agaagtgtac ctaaaagatt gactagagaa tgttcaaata aagcagatag aagaaaactg
     8161 attgtactta aaaagagcac ccacaacaac aggaacagaa agctctggaa aagatactca
     8221 tacatcggca ggattcgtat ctcctgacca atgatcgttc ctacaggata acggaagtct
     8281 cccaaaccat ttttgattat tactagtata gagacgatca aaaatggcat cacgagactt
     8341 agcatagctg caccataata aatggtaaaa cggatgctat gtttctttac catactatcc
     8401 agtaatagat tgactagaga gattacctgc tttctttttt cataagagat gatggaagaa
     8461 agaaacaagg cggttataca taaaatcaaa aaactagaaa tcattcctga aaaaatattt
     8521 tcgtagtaat ttgctaaaga gagatgcgct ggcaagtcaa aaatagaata tctttggatc
     8581 tgatgttctt tcatataacg gagcaactcg acacgttttt gttgttcaat gattggaatt
     8641 ccttgtcttt gaccagaaat cagtcgatcc agtatatctt tttcataatg ataagttgct
     8701 tcgaccattt gctggacatt ccctgtgttt tttgcatgta agattgcttc gatcagtttg
     8761 ttgcttttct ctaaactttc cacaaattct ttctcattct catcttcttt caacgtttca
     8821 atcaaacgtt gcatctcttc atagttatga ttcagttcat cgaattctgt ataagcaagt
     8881 ttatgatcaa aaaaaagtgg gaatgcaaag agcacgacca acaatagagg aaataaaaag
     8941 tttactttgt tttttagtaa gagattgcgt tcaaatgtaa gtaaagacat agttacacct
     9001 catataattt acgataaata gctaaaatct cttccatatt tgaggattct gtgacgattt
     9061 ctccattttt tagaaaacat gctttagaac aaatcgattg gatattcgcg atagcgtggg
     9121 aagaaagaag aatcgtcttc ccctctttct gccattgtct gatgaggcta ttcaagatat
     9181 caatacttcc tgggtctaat ccgttcaacg gttcatcgaa taacatgaca gatgaatcac
     9241 tgatcaagta agcagcaagc aagacatgtt gtttcattcc tagagacatt tttccaatcg
     9301 gcaaagtaag gtactcagtc atcttcaatc gttggaaaac agtttggcat tctactttgc
     9361 tctgccaata gtatttgatc aaagtcaaat aatcaacagc cgatagatag tcggataact
     9421 gattcgttga ttctagaaaa aagaaagacc ggcgataatc tgtataagca gtaggagaga
     9481 tgttgtgaag ggacaattga tcataagttg ttggcagcag ttgggcaatg ccattcagca
     9541 aagttgtttt gccaaatccg ttcggggcaa tcaaagcagc tacttctcct gattctacta
     9601 aaagattcaa ttgtcgaatc acacgttttt taccacgttt catgttcatg tttttgatca
     9661 gtaatgtcat agataggcct ccaatataaa atttaataac ataatagaat ggaaaacatc
     9721 taaattaaat gctaacaaaa gttattgttt gcatattata aaaaaattaa aataacagtt
     9781 ttatttaaaa ttgaagtttt ctgataccga aatcttttta ttttgctgac atactcaaac
     9841 ctttggcaca aagtttcgtg ccaaagtacg actatttctc tttttagtaa gcggatacaa
     9901 ttagagtatc aaaaggagag agggagagac aaaatggaac ggacgattcc caaaaacaac
     9961 atttcgctaa aagcgagggt acaaaaatta ggcagtactt tatcaagcat ggtcatgcca
    10021 aatattggag cactgattgc ctggggtgta ttaacggctc tatttatccc agatggttat
    10081 ttaccaaatg aggctttcgc taccatggta agcccaatgc taacttatct gattccttta
    10141 ttgatcggtt atactggcgg aaaagtaatt gctggtgacc gcggatcagt tgttggggca
    10201 attgcaacga tgggtgtgat tgtcggaaca gatattccaa tgatgctagg cgcgatgatc
    10261 atgggacctc ttggtggata tgccatcaag aaatttgatc agctattcca aaaacggatc
    10321 aaatccggtt ttgaaatgct agtcaataat ttttcagcag gtcttatcgg ttttggatta
    10381 gcgttattag gtttttctgc gattggtcca gttgtcgatg cattgactca agcaatggca
    10441 aaaggtgtag agatcatttt atctgcccat ttgattccat tgacaagtat ttttattgaa
    10501 ccagcaaaaa tcttattttt gaataatgca atcaatcatg ggattttgac accattggga
    10561 actgaacaag tagcagaggc tggaaaatct attctattct tgcttgaagc aaatcctggt
    10621 ccaggacttg gcgtattact tgcttttact ttgtttggca aaggagcagc aaaatcttca
    10681 gcaccaggag ccatgatcat tcacttttta ggcgggatcc atgaaatcta ttttccttac
    10741 gttatgatga aaccgttatt attcttatca gttataggcg gaggaatgtc aggaagtttc
    10801 attttccaat tgatggatgc tggactaaga gcgccagctt ctccagggtc gatcattgcg
    10861 attctagcga tgacaccgtt caattcttac cttcctgtta ttttgggcgt gattgttgcc
    10921 gcagctgttt cgtttggtat ttctgcagta attttgaaag cggatgcgaa agatacaaat
    10981 gaagacttcg aaaaatctgt tcaagagaca aaggcagcta agcaagcttc caaaggtgcc
    11041 caaggtattc ctcaaggagc tacattctct ggcgtacaaa aaatcatttt tgcttgtgat
    11101 gcaggaatgg gatcaagtgc gatgggcgct tcgatcttga gaaaaaaagt acaggaagca
    11161 gacttgcctc aaacggtgac caatcaagca atcagccaac tgacagatga ctcgaataca
    11221 ttgatcgtta ctcaggtcga actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt
    11281 tttgtctcag tagaaaactt tttgaattca cctcgatatg atgaaatcat tcaagaatta
    11341 agcagcaagc agaaaacatc agtttctgaa attgagaatg agccagcaga agagtttctt
    11401 tcagcaaaca tcaaacaaat caaagagatt ctacttgtcc acgatgatcg ttccggatca
    11461 gcaacaatgg ggatgaccgt gctcaaagat atattagaca agaaccattt aactatcccg
    11521 ttaagaaaag tcaatatcca agatttaaaa gatgaacctg attcactgat cgtagccaaa
    11581 gaaagtttag cggaaaaagt acagatggct gcacctcaat ccattcattt atcagtcaac
    11641 agcatggtca atccgcaaaa atacgaatcg attgcacaat atttgaaaca gacagcttaa
    11701 gggaggaaaa cagcatgcaa agtttgccat tcgacatgat cgagctcaat cgatctttta
    11761 aaaccaaaga agaagcgatc cgctattgcg gtcagatgct tgtagaagca gggtgcgttg
    11821 atgaggaata tatcgatgcc atggtagata gagacaaaat gttatctgtt tatatgggga
    11881 attttatcgc gattcctcat ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca
    11941 tctgtgtcgt tcaagtacca gatggtgtag attttggtac cgaagaagaa gaaaagattg
    12001 cgactgtttt gttcggtatt gcaggagtcg gagatgagca tttgcaactt gtacagcaaa
    12061 tcgcacttta ttgcagtgat atggacaatg ttgtccagct agcagacgcg ctcagcaaag
    12121 aagaagtaac ggaaaatcta gcaattgctt aaggagagag aaaaatgaga gcagtacatt
    12181 ttggtgcagg aaatatcggt cgaggattta ttggagaggt tttggcagct aatggatttg
    12241 agattacttt cgtagatgtg aatcacacga tcattgatgc tttaaatgaa agaaaagaat
    12301 acgaaatcga attagcagat gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa
    12361 tcaacaatca aacaaatcca gaaaaagtaa tccaagaaat cgctgaagca gatttagtta
    12421 ctaccgccat tggcccgaat atcttacctt ttattgctga gttgattgca aaaggtatcc
    12481 aagaaagaga aaaggaagga agcacaacat ctttagatat cattgcctgt gaaaatatga
    12541 ttggcggttc agcatttcta gaaaaagaag tgtataaata tcttccggaa acatcattta
    12601 ctgacaaata tatcggcttt ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc
    12661 atgaagatcc tttattcgtc caagtggaac ctttcaaaga atgggtcatt gacgatagcc
    12721 aaagaaaaaa taaaaaaatc caattaaaag gggttttata tgtagatgat ctagaaccat
    12781 atatcgaacg caaactattt agtgtcaata ctggtcatgc gactgtcgct tatacaggtg
    12841 ccttgttagg ttaccagacc attgatgaag caatgcaaga tgcgttagtt gtggcacaat
    12901 taaaatcagt tttgcaggaa acaggaagtt tgctgattgc caaatggggg tttgatgcag
    12961 agcagcatca tgcgtatatt gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg
    13021 atgcgatcac acgtgtcgct cggacaccat tacgtaagtt aggttatcaa gaaagattta
    13081 cacgtccagt gagagaacta caagaacata acttgacttg cccacatttg acggcaacaa
    13141 tggggattat ttttaattac tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa
    13201 tgaagatcca tgaaaacttg gaacaattga ttcaagaagt cacaggaatc aatgatccga
    13261 aaacaatcgg gaatatcaag caaaacgtga accgttatgc caagcaagtc gcttaaaata
    13321 ggatacgaaa aaggggaatg tgacgaaatt tttgtcacat tccccttttt cgaataacta
    13381 ggctgtttgt ccttcaagat agggcagatc caattcttta ccgtaacgtt gttctaaagc
    13441 taggcggatc aaacgagctg ccaggttctc atttcttgca aggataggtc catggaaata
    13501 agaaccgaag acatttcggt aaatgacacc ttcagattgg tcttcaccat tatttccttg
    13561 accttgtacc actttcccca aaggtcgttc accttcacct aagaaagtcc gaccattgtg
    13621 gttttcaaat ccataataag tttcattgaa ttcttcgtta tggataacga tatcaccgat
    13681 aaaccggtta ttttcctgac ttaacgtgta gtgatccagt gcactgattc cttggatctt
    13741 ttctccatta gctcccatgt agtagtgacc tagcaattgg tagccaccac aaatggctaa
    13801 catgacgcca tcattttcga tatagttgat caaagaatct ttctttttct ggatgtcttt
    13861 tgaaatgatt aattgctcaa agtcttgacc accgccaaag aaaaccaagt catatttatc
    13921 agcatcaaaa ggttcatgta tactaacgat ctccgtatgg caagaaaccc ccattttttc
    13981 tgctatatat ttcaacatca aaaggtttcc gttatcacca tatgtgttca tcaaatttcc
    14041 ataaaggtgg gcgatattga gttcataatt agccacggtt cattcctcct ttgataaatc
    14101 cttgagcagc tagttcttta cgaagctgca atacagcagt gtaagtagct aaaatataga
    14161 catgctcagt tggcaattct ttgatttttt cgattacttc tggtaattct tttgtttctg
    14221 tcaatttatt ttctggaatc ccggcaactt ttagacgtaa agtcatatct gtatgacggt
    14281 ctcctccaga aataacagct gggatatcca tatcggcaaa gttttcaaaa ttaccatccc
    14341 aaatccagct gacatcgatt ccgtctgcgt aattggcatt taataaagcc actaaagaaa
    14401 atggataagg agctagacca atcatatcga ttacttgatt gatacctaca ggatttttga
    14461 caagtactaa tgtacatttt ttcccattga tattgatgac ttcttgtcgg ccgaatactt
    14521 tctcgtcata tcctaaacct tgtttgattt tatcaggatc gacattgaag tactctgcca
    14581 ctgcagtagc tgctaaagca ttgtagacat tatacatacc gccaacttcg attccgtatt
    14641 catgaccatc gatcacgaaa tctgctgaca cattgtccat ccgtaccata tcagttaatc
    14701 gataatccaa ttcaggacgt ttgaagtcac aatttggaca gtaatatttc cctaagtttg
    14761 cataagtgat cattttataa tgcaagatgt gatgacaatt tggacataag acaccgtctg
    14821 tattgtaatg agccatttgt tcacgatctt cttcatggtc aaagccataa tattttctag
    14881 gattgactgt ttcgatagaa ttgaagattg gtgaatcacc gttactgata atcgttgcat
    14941 ttggtgactt tgcggcaccg tctacaatca ttttataggt cgtatagatt tctccatacc
    15001 gatccatttg atcacggaaa atatttgtaa ataagaacag ttctggtttc atatattctg
    15061 tgactttgct caaacttgct tcatctattt ctaggacagc aaactttttc tgtccttttt
    15121 ttggttttgc ctgcaagaaa gtcgatacaa ttccctgtac catattcgca ccagtagggt
    15181 tggttagaac ttcatcgaat tgctggcgca aaatattgac tgttaaagcg gtagtcaatg
    15241 ttttaccgtt tgttccggtg acaacgacga cttggtagtc ttttgccaaa ctatctagta
    15301 tatgtggatc gactttcagc gctagtttac ctggtaaact tgatccgcct ttaaaaaaag
    15361 tttttaatgc ccattgcgac gtttttccaa ctgcaatcgc aaaatgactg cgtattccca
    15421 ttatgtaacc tccaaaaacg atttgctgac gaataaattc aactaatcat accatatgtt
    15481 gaaaaaaagg ttaaattgct tttcttattc ttaagaaaag gttacggctg aaagtgcatg
    15541 aaatcggaca aaatcgcatg atttaacaaa aaaacagctt gaaacaaaat gattgtttca
    15601 agctgctgtg attttattat tccctcttac ctatattccc attttaaccg ataataattt
    15661 tctcgcttgg atattcatag cctagatctc gtgtttcttt tggcacgaat aacatcaatg
    15721 tatatagcat tccgatccgc ccaataaaca tcagtgtagc aatcgtgatt ttgcccacaa
    15781 cagacaagtc tccggtaatc cctaaagata aaccagttgt tccaaacgca gaagttactt
    15841 cgatgatgat cgagatcagc gtttgttctt ctgtagcaga taaaaaaacg atacaaaaga
    15901 aacacatacc aagagaaagc ataaagacaa cgactgattt tcgcacatca tcttgatcga
    15961 ttctccgtcc aaaaatattg atgttatctt cacttttgag gaaggaatat agatacaaac
    16021 cgatgattgc tacagtagtt gttcgaatcc cgccgcctac agaactagga ctgcatccga
    16081 taaacatcag taaagaaaaa atgatcagtg ttgtgatttg aaaatcaccc aaatcgtgaa
    16141 tctgcagacc agcattacga gtcgtaatcg aataaaacat cgaattgatc catttgacac
    16201 tcatattgga atcttggaat aaatggtcct tttccaatag ataaatcaag acagttcccg
    16261 aaacaaacaa gataacaaaa gctaacacag ctaatttggt aaacaaggaa aaacgaaacg
    16321 gaagcttcgc attggaacgt ttgtataata gccattcccg gcactccatc aatacaggaa
    16381 aaccaattcc tccgacaaaa atcaaaaaca tgatcaggat caagaaaaaa tagtcatgtg
    16441 caaagggctt gatggaatcc ccagtgacgt caaacccaga gttcgtgaca gccgatatcg
    16501 cctgataaaa accgtaaaaa acggcatcac gccaacgatc aaaatagcct ctgtaataaa
    16561 aataaataga aaagaatgtc ccaaaaagta attgaaacca gattaaaata gcaaaagtga
    16621 tccgaatcag tcggacgata ccagacaaac gcggttgatt catatccgtc atgatcagct
    16681 gtcgctgttt cagtgtgatc cgtcgtttag aaaaaatgac aaaagcagta gaaatcatca
    16741 tgatccccaa accaccaact tgaaataata tttccagcag tataataccg ttatcattga
    16801 acacagaatt gatatcaaaa gtcgatagtc cagtcacact cactgtactg attgccatga
    16861 acagcatgtc cacaaaagaa acatggctat ctggttcgcg aaaaatcggc atataaaata
    16921 gaacgagtga taagaccgtc atcaatatat aataaaaaac gatgatttgg atcgatgaga
    16981 aatgattggc agcaaacttc tgtcctcgcc gctgcagcct tcgaaagaat aaatcaacat
    17041 gcacagtact atcctcctga caatagtcgt ttctaccagt atacaagggg aattaggaat
    17101 actcaaagaa attatcaaac agataacaat acagctttcg attaaaatag aaatggctta
    17161 taataaggaa aatcctagaa aaaagaagga ggaggaataa caaattgatt aatgctcaac
    17221 aaatcgtaga aaaaatgaaa aatcaaaaaa taaattatga tacagttctg aaaaaaatga
    17281 tcatgcaatg ggagaaagaa gaagtaaagc ctaagatatt aatacataca tgctgtgctc
    17341 cttgcagtac gcacacattg gaattcatgt ctcaatatgc agagatcacg ttattttttt
    17401 caaattcgaa tattcatcct aaaagtgagt atttgcgaag attacatgaa caaaaacgat
    17461 ttgtagacca atttaatgag aaaactgacc atcatgttgc gctaatcgtt gatgaatacc
    17521 gtccaaatga attcaccaaa atggttttaa caaaccatct tgaaaacgaa aaagaaggtg
    17581 gtgctcgctg cagtgcatgc ttcaatatgc gattagatct tgttgcgcaa aaagcgcaag
    17641 aactaggata cgattacttt ggaagtgccc tgaccatctc gccaaagaaa aatgcagctt
    17701 tgatcaatca gatcggaatg gatatccaaa agatttacgg gacacaatac ttgccaagtg
    17761 atttcaagaa aaacaaaggc tatgaacgct ctttgcaaat gtgcaaagag tatgatattt
    17821 atcgccaatg ttattgtggt tgcgtgttcg ctgccaagca gcaaggatgc gatctgaaag
    17881 aaatcaatcg ggaagcaaaa gcttacttaa aaacatcaac cgtagttttt gaggaaaatt
    17941 aaagaaatct ttcggaaaaa cgtaagaaaa aaataaagaa tttatggtag aaataaggta
    18001 gactgtataa tttgtccgta gtaaagtaag gaaacaagaa taaatttcaa tgaaggaaga
    18061 gataagatgc gaaaagtagc acctattttg aagccaatcg ctgtattaac aatctcgacg
    18121 attgtcgcga ctactacagg tattgtgaat agttccagct accgtgcacc aagttcaagt
    18181 tctagtactg tagaagaaac aacaccaact acttcagtct cgacatctga accaacaaca
    18241 acggaaagtt tgaatgaaac cactacgaca agtacatggg acgatacaac aactagttcg
    18301 tcgtatgatg agacatatac aactgacagc tcaagcgaaa caagtaatac ggtaccagat
    18361 ggcactccat cggattcaac caatccaagt acaagtccgg atacaagtgc tagctccgaa
    18421 acgataccaa gcagtacgac tgtagatgat aacacgcaaa atgatgatgc agcacgtaca
    18481 gcacagcaaa accaagattc aggaaatacc caaacgtata accagcaagc aggtgattct
    18541 caatgacatt tttactagac cttatcaacg gggtgcataa attccttggc tatttagaca
    18601 tcagtcctaa atatcttaac cgtggatata cgattttaag tgtgattccc acgctttatt
    18661 tattgagaat tgtttatgga ctttggcaaa accaaaacta tcttcaattt ttcttatatg
    18721 gacttgcatt tctagtgctt gtttatttta ctgttttaaa tgtattttat tactttttag
    18781 ataaaaacac gaaagccgat gtcactcagc tttttgtgaa gtatttacct gatgaagcgt
    18841 tcaacatcca gacagaagaa tcgatagata atggaaattc gatcgataaa gtgaatacac
    18901 aagaggtcat ggtttcttat gacgaagatt atcaattgaa attagccgag aatatgcggt
    18961 atttgattgg aaatggtgag atcaaaacaa atgaactcgg tcgcatggat ggatttctgg
    19021 tcgatagaaa tacattgtat ccttattact atgtcaagaa aacaggagag aaagagtatg
    19081 ttctaaatat tggacgaagc tactcggaca tggaaaaagt agggaccatt cgacttgact
    19141 caacagaaga cacattggaa cctgtcggat tgttcatcgt cggcggtgat tttgtcaaag
    19201 acgggttacg ctatcatgag ccttatcgtc tgaaattgat tgcaaaaaag gcacagtcgc
    19261 aggaagaacc agtggtttat agtcgtagcc aaagaagaag acattcataa tgtgatgaaa
    19321 cgccgtgtat cagtggttgc gataaaaacg tttattcgga ataaggggga tgtgacacgt
    19381 cttttgtcac gttccttttt tatataacga gaaagataag acgaacacat cacttattct
    19441 ttcctttcat ttataaacat tgtagaataa agaaagtaca acgaagtatt gtgaaaaaac
    19501 gtaataaata acgaggaaaa tacaagtagg aggtaagctg tgaaagaatt tttgaaaaat
    19561 tgggggattt tgatccttgt attagcggct attttattag ctagagtgta cgtgtttaca
    19621 ccagttactg taaacgggca ttcgatggac cctacattaa gtgatggaca acgattgatt
    19681 tcatctaaga tctcaaacta tgaacgaatg gatattatta ctacaaagga accgggggat
    19741 gaagaacgga tgattgttaa acggatcatc ggtatgcctg gagatacagt caagatggaa
    19801 aatgaccagt tgaccattaa tggcaaaaag tatgatgaac cttatctgga tgagttcaaa
    19861 aaagaatttt cagaagataa gttacaagga gaatatgctt atagctcagg attccaagca
    19921 caagcagaaa gttcatccac ctttacgagt gactttgaat atactgtgcc aaaaggaaag
    19981 tatcttgttc taggcgataa ccgcttgatt tcaaaagaca gccgaatgtt tgggttagtc
    20041 gacaaagata tgatccaagg aaaagttgtt ttccgttatt ggcctctttc agaaattaaa
    20101 atcatgtgat gaaaaaataa aaagaagctg tgacatgttt tttgtcacag cttctttttc
    20161 cgaaaattcc ttcttatttc ttggagttgc tcactttttt tgcaatctca ttttaagctt
    20221 tttggctgat ggataagaaa agcgaagcgt ttttgttcat aaatagcttt tccccacgat
    20281 ggaaggtatg actagtagca gagacaaagc ccgttttttc caatcgatta ttcaattctt
    20341 cttggctaaa gccattatgg actttcggat gactaatttg ttcattcttg tcaaagtcta
    20401 cgatgatcaa ttgaccgcca ggagctaaaa tttcataaag ttttgtaaga atattctccg
    20461 tatcaggaat gtgtaaaagt acaagtgata gaaggatgag attagcttgg acagcaggaa
    20521 attcgacaga agcatcagcg tggatcgttc ggacattttt cagatctgct gcttggatct
    20581 tttcttctgc cattttcagc atagtttctg aagcatcggc aatgatcagt tctttgaatc
    20641 tttccgcaag cggtaaacta acaagcccag ttccgcctcc gtaatccagc aggacttttt
    20701 ggtggctgtc gcgcggtaga taagtagtta gttcttcgcg aataatcttg gctaatgctt
    20761 gacggtcagg ggaatcataa tggttagcga tggaatcaaa aatatttttt gtcatcaaat
    20821 agctccttac gtaaaacttt gaaattttaa cttattatag cacgtgaaaa gaaagaaagc
    20881 atgctatact caattcaaaa agggagaatc tagaaagaat atgccgatat cgtttgtaaa
    20941 agatagagag gaaaaaggaa agtgtgtccg agaaatatta ctagacttgc ctgagtggtt
    21001 tgggcttcct gaatcaacgg aaaaatatat cgaggaatca agtaaactac ctttatggtg
    21061 tgaaaaaaga aaagaagaat atttaggctt tatcactctt tcacagacaa gtgaagatac
    21121 tgccgaaatc tattctattg tatgggaata aaaaaagact atcatcacca aggattagga
    21181 acaaaacttt ttaaagaagc agaaggttac gcagcaaaac attacaaata tttgcaagtg
    21241 aagactgtag acgaggggca ctactctata tatgatcaaa ctatttgttt ctacgaatca
    21301 ttaggatttt caaggttgga agtttttcca aatttatggg atgaatggaa cccatgttta
    21361 gttcttgtca aaaaattgga acaaaagtga gcaatagatt taatactaat ctgtgcttga
    21421 tgaaaattct cactgtaaaa aatgctgaaa atcaagtata ataccatagg aacaaactgg
    21481 aaggtgaaaa agagcatggc acaactattt tttaagtatg gcgcaatgaa tagcggcaag
    21541 acgatcgaga tcctaaaagt cgcacataat tatgaagaac aaaataagcc agttgtctta
    21601 atgacaagcg gtattgatac aagagatggt ttaggcgttg tttcaagccg aatcggactg
    21661 aaacgtaatg cgatgccgat atttgaagaa acagacgtat ttgacgtaat tcagaaaatg
    21721 gaaagcaaac cctattgtgt gctgatcgat gaagcgcaat tcttaaaaaa gaaacatgtc
    21781 attgctttta cgagaattgt ggacgaattg gatattccgg ttatggcatt tggtttgaaa
    21841 aatgattttc gaaacgagtt atttgaagga tcaaaatatt tattgcttta tgcagacaaa
    21901 atcgaagaaa tgaaaacaat ctgttggttc tgccacaaaa aagcaatcat gaatttacat
    21961 tatattgatg gaaaccctgt ttatgaaggc gatcaagtgc aaattggcgg aaatgaagct
    22021 tattaccccg tttgtcgaaa acattacttt cacccatcaa tgattacaga aggagaataa
    22081 gcatgtacga tcaattacaa gcaattgaag accgttatga agagcttgga gaacttttaa
    22141 gtgacccaga agtgatttct gatacaaagc gttttatgca gctttcaaaa gaagaagcaa
    22201 acacgcgcga aacagtcgaa gtttatcgtc gttataaaga agtagtacaa ggaatcaccg
    22261 atacagaaga actgcttggt gaaaaactgg atgatgaaat ggctgaacta gcaaaagaag
    22321 aattatctga attaaaaaaa gaaaaagaag tcttagaaga aaaaatcaag atcttgcttt
    22381 taccaaaaga tccaaatgac gataaaaata tcatcatgga aatccgtgga gcagcaggcg
    22441 gagacgaagc agcattattc gctggcgatc ttttcaatat gtaccaaaag tatgctgaag
    22501 cacaaggctg gaaaacagaa gtgatggaag ctaacgttac cggaatcggt ggatataaag
    22561 aagtgatcat gatgatcacc ggagacaacg tatattcgaa attaaaatat gaaagtggtg
    22621 cgcaccgcgt ccagcgtgtt ccttcaactg aatcgcaagg gcgtatccat acttccaccg
    22681 caacagttgt cgtgatgcca gaagctgaag aagtagagat cgatattgaa gacaaagata
    22741 tccgtacaga tatctatcat gcttcaggtg ctggcggaca gcacgtcaat aaaacagcat
    22801 ctgctgtacg tttgactcac ttaccaacag ggatcgttgt tgctatgcaa gatgaacgtt
    22861 ctcaaatcaa gaaccgtgaa aaagcaatga aaatcttgcg tgctcgtgtg tacgataaga
    22921 tccagcaaga agcacaaagc gagtatgatg ccaatcgaaa atcagcagtc ggaactgggg
    22981 atcgttcaga acggatccgg acgtataatt tcccgcaaaa tcgtgtgact gatcatcgta
    23041 tcggattaac tatccaaaaa ttagatcaga ttttagcagg gaaattagat gagatcgttg
    23101 atgcattgat catgtatgat caaacatcaa aactggaagc tatgcaaaat gggtaaaatg
    23161 acctatcgag aagtcctgtc acgggcttct tcttttttag aggatcatgg gaaagaagca
    23221 tatagtatcc aatttttatt tttggaacga aaacaatgga aaaaacttga ttggctcctt
    23281 catatgaacg aagagatttc agaagaagaa cagcggttga tcgaaaccga tctgcgactt
    23341 ctgcttgcag atcatccacc tcagtatttg ctaggatatg cagattttta tgatcatcgg
    23401 ttaaaggtaa ctgaagcaac actgatccct cgaccagaaa cagaggagtt agtcgaatgg
    23461 tgtttagacg agacgcctgg tgtaccgtta gaagtgatag atattgggac agggactgga
    23521 gcaattgcta ttagtttgaa agcagcaaga aaaaattggc atatatctgc tgtggatctt
    23581 tcagaagaag cattagaagt agcgaaagaa aatgctcaga aagaaggaac gaaaatcagt
    23641 ttttatcatg gagatacatt aggaccagta atggatcagc aatttgatgt gattatttct
    23701 aatccgccgt acatcagtcg gaatgaatgg gaactgatgg atgaaagtgt ccggtcattc
    23761 gaacctaaaa tggccctttt tgctgagaac gatggattag ctatttatga aaaaatagca
    23821 gttgaagcac caagcgtact aaaatcaaat ggaaagattt ttttagaaat cggatttcgc
    23881 caaggtgaag cagtaaaaaa aatatttcag caagcatttc cagataaaaa agtggcgata
    23941 aaaaaggatc tttttggcaa tgaacgaatg atccaagttt atggttgaag ttgtggatga
    24001 aaaatttttt atttgttttt ttattataaa aaaacactta tttgacaagg atgtaaacga
    24061 ctttttggtt tatcaacaag tttatccact attgacaaat agattatcca caaacagtgg
    24121 aaaacttgag gataaactca gacaaaataa gaatgtatga aaaaggggtt gtggataaac
    24181 tgtggaaacg aaacgatatg atgaaacaga attgcaagag gcagccgaag cactgaaaga
    24241 aggagaactt gttgcttttc ctaccgaaac agtatacgga ttgggcgcca acgctctttt
    24301 accaaacgct gtcaagaaag tgttttctgt aaaagggaga ccgcaagata atccattaat
    24361 cgtccatgtc gcctcttttg aacaagtaaa agagtatgtg gacaactttc atccggcagc
    24421 agagaagatt gtgaagaact tctggcctgg accattaact ttgatattta agatcaaaaa
    24481 agacactttg ccctcagttg tgacgggagg attatccaca gctgcatttc gtataccgga
    24541 taacaaaaaa acactagaag taatcgaact ttccggtgtt ccgcttgtcg gtccaagtgc
    24601 aaatacgtcg ggaaaaccaa gtccaacgac tgcagatcat gtatatcatg atctacaagg
    24661 aaaaattacc gggattattg atgatggagc tacgcgaatt ggtgtagaat ccactgttct
    24721 tgatttaagt gatccaacag ccatgcctat gattttacga ccaggtgcag taacgaaaga
    24781 acaaatcgaa gcagtcatcg aatctcctgt tgctattgat cagcatcttg taaaagaaaa
    24841 tgaaacacca aaagcccctg ggatgaaata caaacactac tcacccgata cacgtgttct
    24901 gatggtcaga gatggcgatt ggtcaactgc agttcaatgg gcaaaaaata agaagatccg
    24961 tgcaggtgtc atagcaagcc ccgaaattgc ggatcaagtg cggacagata ctgcagctgt
    25021 ttatatgtat aacgacaact cagtagaagc ggcggctaaa ggattgtttg caggtctaag
    25081 aggattagat gaaccaacac ttggattaga tttgattttt gtacaagtgt atccagaaac
    25141 tggattgggg aatgcttaca tgaatcgctt gaaaaaagca gcaggacaaa attattttga
    25201 aaaatgatca attggttatt gtcgcattct tttagtatga tacaatgcta tcaagtaaat
    25261 cttctatcag tgaacgaaaa actaagcgta actaatcgta atagaagaga tacgaatatt
    25321 tggcaatttg tttttgatgg gaaaaataaa attgtaggaa aataaaattg taggaaaata
    25381 aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt tgggctgcga
    25441 tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct gaaaactttg
    25501 tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac gcagaaggtt
    25561 atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa aatttagcga
    25621 ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct cactccggct
    25681 ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg atcttaggaa
    25741 tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc agtggcaaaa
    25801 cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat tataatgtgg
    25861 tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc agtgcttacg
    25921 gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc gcaaaactga
    25981 tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct aatccagttc
    26041 cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct cgaggcggca
    26101 tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta tttcctggta
    26161 ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc aaagaagcat
    26221 tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa gcaatggtca
    26281 aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat aatcatctga
    26341 tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc attttggaca
    26401 gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt ccattcaaaa
    26461 caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa gaagaagatg
    26521 ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat aacgctcaac
    26581 tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg cataaaaaat
    26641 aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc tgtttatttt
    26701 ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga ggtgtaattt
    26761 tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct cgttagaaaa
    26821 ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg aactagcacc
    26881 ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca aatattccgt
    26941 cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata ttcgtctcgt
    27001 acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg gagcaggctg
    27061 ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg ttcgtacgaa
    27121 ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc caacagcaat
    27181 tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa aaaatctgga
    27241 tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta cagtcatgac
    27301 gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt attatccagt
    27361 cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact attttttgac
    27421 aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag agcgtgctga
    27481 aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc cttcagcaat
    27541 gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg cgcgcaaact
    27601 tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc atgattttgt
    27661 catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat tatctacttc
    27721 ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt ttcaaaaacg
    27781 gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg ttttacaatt
    27841 aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa gtgatcgatc
    27901 atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga acaaaggttt
    27961 ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca cgggatatgc
    28021 cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa acgttgtctg
    28081 gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt gacggtatct
    28141 tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat gaagaaacat
    28201 tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt cagttgtttg
    28261 tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat gcattgaaag
    28321 aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag ggagtaaaag
    28381 ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat gaaaaattga
    28441 atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg tttggtacaa
    28501 aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt ttggtcattt
    28561 ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa aaaaccatcc
    28621 caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt aacgattact
    28681 acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat ctcaacaaca
    28741 gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg aattgtgaac
    28801 attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat agttgtatca
    28861 aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta taccacgttt
    28921 tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa tcagccaaat
    28981 agagtatata atttcttttg gataaggcca gcttagctca gttggtagag caacgcactc
    29041 gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa gacagatata
    29101 tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat aaagtatgct
    29161 ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa acgactagca
    29221 atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc gagttgcttt
    29281 taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg atgtgtgcta
    29341 cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca aagatgattg
    29401 ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg agaaaataca
    29461 ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat aacgggctga
    29521 aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc aaaaataccg
    29581 agactgcggc actgattact acattgataa aaaaatattt ggctatattt ttgacaggag
    29641 cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt gtaaatgcta
    29701 taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat agcaacccta
    29761 acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct attttttttc
    29821 ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata gagtcagtat
    29881 acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct aaaaatttat
    29941 cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag caaaaccaag
    30001 tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt caatcatttc
    30061 aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc tagcattttc
    30121 taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat ctgcgcttga
    30181 taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat ttacatagtc
    30241 cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt tagtacctgt
    30301 taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata tacagcctga
    30361 aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat cattggaagc
    30421 aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta tttggttttc
    30481 gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt catagatatg
    30541 aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca tatttaatta
    30601 cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta gcagaaaatg
    30661 agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa cgttcttaga
    30721 attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa taaaaatttt
    30781 tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag tgtattgaaa
    30841 ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac tttgtttata
    30901 gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa agtgaagaaa
    30961 tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc aaagaaaagg
    31021 aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat tatcctaaag
    31081 tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa ttgtctaaac
    31141 cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa gaattaggtc
    31201 gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt attcatctca
    31261 atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat ttgaacagag
    31321 tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta ttttatcctt
    31381 taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg tattttatga
    31441 aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa tatagattga
    31501 a
//
LOCUS       DASJST010000018        31325 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJST010000018 DASJST010000000
VERSION     DASJST010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.018"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..603)
                     /locus_tag="U4B05_000631"
     CDS             complement(<1..603)
                     /locus_tag="U4B05_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEN2049786.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELV"
     gene            complement(693..1238)
                     /gene="hpt"
                     /locus_tag="U4B05_000632"
     CDS             complement(693..1238)
                     /gene="hpt"
                     /locus_tag="U4B05_000632"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN2049787.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(1238..2683)
                     /gene="tilS"
                     /locus_tag="U4B05_000633"
     CDS             complement(1238..2683)
                     /gene="tilS"
                     /locus_tag="U4B05_000633"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN2049788.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(2803..3273)
                     /locus_tag="U4B05_000634"
     CDS             complement(2803..3273)
                     /locus_tag="U4B05_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN2049789.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(3319..3744)
                     /locus_tag="U4B05_000635"
     CDS             complement(3319..3744)
                     /locus_tag="U4B05_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN2049790.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(3811..4080)
                     /locus_tag="U4B05_000636"
     CDS             complement(3811..4080)
                     /locus_tag="U4B05_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN2049791.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(4091..5686)
                     /locus_tag="U4B05_000637"
     CDS             complement(4091..5686)
                     /locus_tag="U4B05_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2049792.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALCRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(5701..9222)
                     /gene="mfd"
                     /locus_tag="U4B05_000638"
     CDS             complement(5701..9222)
                     /gene="mfd"
                     /locus_tag="U4B05_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN2049793.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(9239..9799)
                     /locus_tag="U4B05_000639"
     CDS             complement(9239..9799)
                     /locus_tag="U4B05_000639"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN2049794.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            10152..11126
                     /locus_tag="U4B05_000640"
     CDS             10152..11126
                     /locus_tag="U4B05_000640"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2049795.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            11296..12696
                     /locus_tag="U4B05_000641"
     CDS             11296..12696
                     /locus_tag="U4B05_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN2049796.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(12774..13496)
                     /locus_tag="U4B05_000642"
     CDS             complement(12774..13496)
                     /locus_tag="U4B05_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2049797.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(13575..14081)
                     /locus_tag="U4B05_000643"
     CDS             complement(13575..14081)
                     /locus_tag="U4B05_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2049798.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            14204..16003
                     /locus_tag="U4B05_000644"
     CDS             14204..16003
                     /locus_tag="U4B05_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN2049799.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(16071..17645)
                     /locus_tag="U4B05_000645"
     CDS             complement(16071..17645)
                     /locus_tag="U4B05_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2049800.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(17900..18409)
                     /gene="mreD"
                     /locus_tag="U4B05_000646"
     CDS             complement(17900..18409)
                     /gene="mreD"
                     /locus_tag="U4B05_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN2049801.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(18413..19267)
                     /gene="mreC"
                     /locus_tag="U4B05_000647"
     CDS             complement(18413..19267)
                     /gene="mreC"
                     /locus_tag="U4B05_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN2049802.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(19422..20060)
                     /locus_tag="U4B05_000648"
     CDS             complement(19422..20060)
                     /locus_tag="U4B05_000648"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN2049803.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(20061..20711)
                     /locus_tag="U4B05_000649"
     CDS             complement(20061..20711)
                     /locus_tag="U4B05_000649"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN2049804.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(20724..21623)
                     /gene="rsgA"
                     /locus_tag="U4B05_000650"
     CDS             complement(20724..21623)
                     /gene="rsgA"
                     /locus_tag="U4B05_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN2049805.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(21788..23863)
                     /gene="pknB"
                     /locus_tag="U4B05_000651"
     CDS             complement(21788..23863)
                     /gene="pknB"
                     /locus_tag="U4B05_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN2049806.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYNYSDTVPSGSIISQDPASGEVVAEET
                     YINFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVP
                     GAGTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSS
                     SASTSESSK"
     gene            complement(23860..24600)
                     /locus_tag="U4B05_000652"
     CDS             complement(23860..24600)
                     /locus_tag="U4B05_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN2049807.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(24613..25971)
                     /gene="rsmB"
                     /locus_tag="U4B05_000653"
     CDS             complement(24613..25971)
                     /gene="rsmB"
                     /locus_tag="U4B05_000653"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN2049808.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(25971..26909)
                     /locus_tag="U4B05_000654"
     CDS             complement(25971..26909)
                     /locus_tag="U4B05_000654"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN2049809.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(26902..27393)
                     /gene="def"
                     /locus_tag="U4B05_000655"
     CDS             complement(26902..27393)
                     /gene="def"
                     /locus_tag="U4B05_000655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2049810.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(27408..29816)
                     /gene="priA"
                     /locus_tag="U4B05_000656"
     CDS             complement(27408..29816)
                     /gene="priA"
                     /locus_tag="U4B05_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN2049811.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(30069..30374)
                     /locus_tag="U4B05_000657"
     CDS             complement(30069..30374)
                     /locus_tag="U4B05_000657"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2049812.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(30379..30993)
                     /gene="gmk"
                     /locus_tag="U4B05_000658"
     CDS             complement(30379..30993)
                     /gene="gmk"
                     /locus_tag="U4B05_000658"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN2049813.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         8594 a         6773 c         5775 g        10183 t
ORIGIN      
        1 aactagttct tgtttttctt cttccgcacc cgctacatca gaaaaacgta cgcgattagc
       61 ttttttgtct gcttctttgg cttttgattt accaaagttc atgacacggc cgccaccgcc
      121 accgccgccg ccttgttgtc ccatcatcat gtaaaagaaa aagatgatga ttacgattgg
      181 caagaagcta agcaataggg aaacccatat accgctgctt gattcttctt ttactgttgt
      241 agcaactttg ttttcttttg ccagttctgt tacttggctc aatgttgtat catttggcaa
      301 aatgattgtt gtgaaatggg tagtagtcgt atctgttgaa cctaagatag ctaacccacc
      361 gctgttagcg acggtctgct gttctttgta ttcaccagtg atcttatata caccatttgc
      421 aggctgcact tcaaattctt tgatcttgcc tgcttgtagt tgttctgtga attttgagta
      481 ctcgatatct gacgatcgct ggccgttatt accaaaaatg aaataaacga ccattaccat
      541 cgctaagatt acgaggacat aatacaaggc atttttcatc atgccgttgt tctttttgtt
      601 catagttgcc ctccctaagc gtcaatttaa tttaacagtt ttttatcaat cctgaccatt
      661 ctattgtacc ataatttaat tgagatacag cattaatttg attgataaac actaggtttc
      721 aatacaccga tgtacggaag gttacgatat gcttctgcat aatccaagcc ataaccgacc
      781 acaaattcat ttggtacgtt gaagccaaca taatctgctt cgatatttac tactcttcct
      841 tcaggtttgt caagtagtgt aacaattttg actgattttg ctttgcggta tttgaaaaga
      901 tccactaaat aagcaagtgt tcttccgctg tcaatgatat cttcaacgat caacagatcg
      961 cgaccttcta cattcgtatc taagtctttg acgattttta cttcccctga cgagactgtt
     1021 gcattcccgt agcttgaaac atccatgaaa tccaattcca aatacgtatc gatagaacga
     1081 acgatatctg ccatgaatgg taccgctcct tttaagacac ctactactag gggattggtg
     1141 ttttggtaaa tttctgttaa ctctttgcct agttctttgc aacgaacttg tatctcttct
     1201 tgtgagatca aaattctctc aatatccttt tccaacatca ggttctcctt ccaattgctt
     1261 ctttttgata tttgtaaaga agtatgtagt gtattttatc agtttcttca cgaatactca
     1321 aatatgaaaa ggcaaaaggt atcaagctat atatatttct tttggcatct gtaatgatcc
     1381 aagcatgctg cctttgtgaa ttaggtactt tctgatcgat aaaatatcta gatacttttt
     1441 ttgtgagatt ctctgttaat ttaatgcggt ctcctgcttt tcttttttcg acatacagtt
     1501 gcacatcagc aggaatccaa atgtcatgag aaaatagaga ccatgaatct gttgctttga
     1561 tgtcccctgc attttcaggt ttgaccagac caatccattc tgtttcattc aaatatattc
     1621 cttgttctgg aatcagcaac aagcgctggt cttctgtctg gctagttgct tgtttttctt
     1681 ttttagagag tacaacaaaa tgatactctc ttttcagtat ccaattattg ccaatactga
     1741 gttgccattg gctttttttc tgttgccact ggtctaaaat cgactccatt tgttgttctt
     1801 tcaaaataat tcctgttttt gagaatgctt tgtcgaaaaa ggagtaaaaa aaatagtatc
     1861 gttcagcatc tgtcatttct tcaatcattt ctgcagaaaa tcgaaattgt tcctcccact
     1921 gttcgacttc cttttcgatc aactgtccca tatatttttg aatgacttgg tctgcccatt
     1981 ggatctgttg cgaaaattgc tggaaatgtc tcaacgcctg tgtattttct tgtttcaatt
     2041 gaggaactac caaatgacga agccgatttc tctggatatc taacaattgg tttgtctgat
     2101 cctcaaagta aacaagctgc gattccgctg cataagtata taggtcttct ttgctaaaac
     2161 tcagcaacgg acgaatcagt ctgcccgtag caaatggttg tacttctttg attccagaaa
     2221 aagtatttaa ctgtccccca cgaacgattt tcattaacac cgtctcgatt tgatcatctc
     2281 cgtgatgagc agtcatcaac gtgtcatatt gtccttcttc catcacgcga gtgaattctt
     2341 gataacgaaa gttacgagct tctgtttcta tggcggattt ggctggatct tcccaaatac
     2401 ttacatataa agggagattc ttctcttcac aatatgagcg aagataggca gcttcttctg
     2461 ctgattcttc tcttaattgg tgattgatgt ggacgatacc taaagaaaaa ccgatttggc
     2521 gttgcacttg taaaagtaaa tggagcaaaa ccatcgaatc aacaccacct gataccgctg
     2581 ctaaaattct cgatccttgt tgccagaatc cttgttcatt cccgtgccga ataaatgact
     2641 gctgtaatag cggtgctttt ttcccgcctt tttcaacgtt catcctttcc acaccctttc
     2701 ttcctaaaaa aaagagagcg ggaaaacgtc taaaacgaca attaacctgc cctctgttga
     2761 tactatgacc caaatagtcg cactatcatt atttgcggct ttttagctgc gacgtccgcc
     2821 acggccaccg cgctttcctt ctgtgtttcg cttcaatgaa gataagcggt catcgctatc
     2881 tttcaaaaat gaactcatca atgaatcgaa gtcttctttt tgagctgaag gttgactgcg
     2941 agaaaaagat tttttctgtg ctggacgatt tcggttgtca cggggatctt tttgttgata
     3001 ttttttagga cggctttctt ctccagattc agcaggttgt tcctgcgctt tccggataga
     3061 tagtccgatt tttccatcat catttactga aataacttta actgttactt catctcctac
     3121 acttaaaaca tcatggatgt cttttacata gccatttgac acttcgctaa tatgaactag
     3181 acccgttttt ccagcaccta gatcaataaa cgcaccaaaa tttgtgattc ctgatacttt
     3241 tccttgcagt tttgctccta cctcgattga cataaaaaaa atgttcctcc taattatttc
     3301 ccttttttat attcctgctt attttgcagt tgtagaacta gttgattgat ctgtttcatt
     3361 tgtcgagctg tctgtatttg attctaatat aggataaact ttttcaccgt cttttgagta
     3421 aaagaatttg cttctggcaa gtttagctac atactcatca tctttcagca atgaaacatc
     3481 ttggtgcagc tgatcaacgt ctgcgttgac ttctttcatt tcagtatttg cttcgactct
     3541 caattcgttc aattgatgca tcctttgcat gtcatggaaa atctgcacac ctattatcgc
     3601 gaaaatgacc aaagcaccaa caaaaattgc agccagtctg cgtcttcgaa aaatcagttg
     3661 acgttgttgt tgctgaaatt cagcatactt tttctttgca tattccgtat ctagcgcagc
     3721 gattttattg ctttttttcg tcacttggat tcgctccaat ctttttgatt gtctttttca
     3781 ttataccaat atcgtgaaag cttgtctaat ctatttctct agtgtttctg tgcgattttc
     3841 agaaataatt tcgtacattt tttgtgcatc ttcttttttg gttgattcat gtaattccgt
     3901 aattttcact tccaacgttt tgttgccaaa ctggactttc aaaatatcgc ctactttgac
     3961 atcactggaa gatttcgcca atttaccgtt gatttgaatt cttcctttat cagctacttc
     4021 ttttgctact gatcgtcttt tgatgattcg ggaaattttt aaaaatttat ctaatcgcat
     4081 atcttattcc tcacttttta atttttttat ttaatcgcag gattttctta ccaaaaggca
     4141 acattagcca ttctcgtact gtaaacagac gaagacgaat cactgcataa atgaacacag
     4201 aagccccgat cccaactcct atcacgctga caaaaaaagc agtggaacga tgagttacag
     4261 gaccaaatac tatattcaat acaccataat acagcagcag tactactgtc atacaaagaa
     4321 ggctgaacat caacttccgt ccaaaatgcc gttctttcca aaaagcattt agctcttttg
     4381 tttcaattcg aacaaaatac caaagcgtca cacctaatcc taaaagggta gacaggcttg
     4441 cacctgctgt tcctaataaa ccggtcagaa aagaagtagt caatcctttt accaatagtc
     4501 cccatcctgc gccttttaaa gaagggcgaa acgagttctt gctttgagca atactttgat
     4561 aagcttggat gactgcagtg aaagcaatgg aaaaaacaaa taatacaagc gctgcatttc
     4621 ccgcataatc tttaaacaac gcataattga tataagggag cagcaaggct aatcccaaag
     4681 acgcagctaa tgctaaagcg gtcgtcaatc taagatagat tttggctgtc tgtaaaaact
     4741 gacgatagat ccggttggtc aaatgacgag tcaacgcagg caggaatgtc gcacttaatg
     4801 cagtggcaat cactaaacct aattgtacta aaggctgacc tcgatcatag accccttttg
     4861 ctatcttcgc accgtattct gacagtccgt aaacttccaa agcatttttt acaaagaaag
     4921 agtcgatcag ctggaaaaag ataagcaatc cactataaat cgaaaccagt ccgccttcta
     4981 ccagaaaacg ccggatcaag ggctttgacg gctgtttgaa caatggaaac cgtcggaagc
     5041 ttaacgctcc tccatgaatc ttttgttcat agtagtgaag gatcccatat gcgcataaac
     5101 cgccaaaaac agctccactc attgctgccg tccctgtttg gtacaccgtc catccaagct
     5161 ttctaaaagc aaacgcacta cagacaatca caaaaacacg aacgaactgc tcaactactt
     5221 gagaaacagc agttggttcc atcaagaatc ttccttggaa attccctcga taaaaagata
     5281 gaccaggcat cagcaaaaag gtaaaggaaa ctacttggat cagcggtttc aattgtatgt
     5341 ctcccatcat ccaagcaatc cagctgctaa atgcaaagac aaacaaccac aaaaagacac
     5401 ctgtccaaaa tactaaggga taaagatttt gcaaagcttg ctttcgctcg aaaggattct
     5461 ttctttctgc aacgtacttt gatatgaact gcggaagacc ggataaagct aaagtcattg
     5521 ctatcccata aattggataa acttgctgat aaacataaaa accttcgtcg ccgactaagt
     5581 tttggagggg aattcggcaa agggcactta atacttttgc tatgaaagaa gcaatcgtca
     5641 atacaaatgc tccttgcatg acgcgccgca tttctttatg atccaaatca ttgctccttc
     5701 ctacgctgac agatattttt gctcacgcaa cgctttgaca aacttttgga tttcctgaag
     5761 ccatactgct tcttccattt ggttggggat cgtcaagcgt atcaccatct gttctttttc
     5821 aacgcctaga ctggctttta gttttgtcgc tgtcagtgct tcgaataact gttccacact
     5881 atagcgtttt gttccaactt tacttagagt aaagactata tgcgcctcct gtttccttat
     5941 cgtttctagg agcgctcgat cgccatccat tttgatctgc ccaattgtca agagatgtgc
     6001 tacttcttct ggatattctc caaaacggtc gagcagatca tcttctagtt catctagcat
     6061 ttcttgcgaa tcaagttcac gaattcgttt atagatctcg attttctgcc gttgatctgc
     6121 cacgtatgtt tcaggtagat acgcatcgac accgaggtcg atctcaacag tggtcttctc
     6181 cacttgacta ttctttcctt gtttacgaga aacagcttcg cttagcattt gagaatacat
     6241 atcgaaaccg actgcatcaa taaatccatg ctgctgagca cctaagagat tacctgcacc
     6301 gcgaatggaa agatcccgca tcgcaatctt gaatccagat cccagttctg taaaatcttt
     6361 gattgcctga agacgttttt cactgacttc gttaaggatc ttctgctgct catacataaa
     6421 gtaagcatat gcgacacggc tgcttcttcc tacccgccct cgcaattggt ataacgtgga
     6481 taatcccata taatcagcgt tctcaacgaa caacgtattt gcgttcggga tatctactcc
     6541 tgtttcaatg atggttgtag tgacaagaac atcatattga ccttcaataa attcaaaaag
     6601 cgtattttct aattgcgcct ctgtcatttg cccatgagca taaccgatcc gtgcttccgg
     6661 aacgagttct tggatttctt ctactttttg ttcgatcgtt tctacccgat tatataaata
     6721 gaatacttgt ccacctcgtc ccatttctcg atggattgct tcgcgaatag ccccaggatt
     6781 tttttccata acatacgttt ggatcggata acgattcgca ggcggtgttt cgattactga
     6841 aagatcccgc acgcctaaca tcgacatatg caaagttctc ggaatcggtg ttgcagtcag
     6901 agtcaaaaca tccacttgtg cacgtaactg tttcaatcgt tccttatgtt ttacaccaaa
     6961 acgttgttct tcgtcaatga tcagtagacc caaatcgcta aattcaatat cttttgacaa
     7021 aattcggtgg gttcctacaa caatatcaac ttgacctgta cgcagttgtt cgatggtttc
     7081 tttttgttgc tttcttgttc tgaaacgact taatagcccg atatttacgg ggaaaccttc
     7141 aaatctttcc agcatcgttt catagtgctg ctgtgccaag atcgttgttg gaactaaaaa
     7201 ggctacttgt ttgctttcct ttatcgcttt aaaggccgct cgaagcgcta cttctgtttt
     7261 cccataacca acatcaccta cgagtagtcg atccattggt ttttcttttt ccatatcccg
     7321 tttgatctcc gctgcacttc tcaactggtc atcagtttca gagtagggga aggcattttc
     7381 gaattctttt tggtaggcgt catcaggtcc aaaagcatat cctttttctg actctcttgc
     7441 ggcatataat ttgattagat cgtcagcgat atcctcgatt tttgaagaga cttttcgctt
     7501 ggttttcgtc cattcacttc cgcctagctt attgatgcga ggcgctttgg cttcagaggc
     7561 gacatatttt tggatcagat tcagttgagt aacaggaata aacagtttgt cgtcgttttg
     7621 atacaaaata gtcatatagt cttggtgaac gccatctact tctagcgttt ccatcccaat
     7681 gtatttcccg ataccatgat tggcatggac aacgtaatct cctgctttta actcattata
     7741 gcttttcaat cgctctgcgt ttgttactgt ttgacgtctc gtttgttttt tcgtcgtttt
     7801 ttggaaaatt tctttttctg tgatagctac tattttttct tgaggaagtt caaaaccagc
     7861 ctgcaaagct ccttcaacta attgtacctg tcctctgatc aatgcatttt tttctgtttc
     7921 tactactaat atgtcttcat ctcgaagcat ttgttctgct ttgcgtattc tgtcctttgt
     7981 cggaataaag atcacaacag tctgtttttg cttgcgccaa cggtccattt ctgttttcag
     8041 caatggcatt tgtccgaaga actgttgcat cgtccgatat tgaaaattat ggattgcttg
     8101 gaaacgaaga tttcccatcc ctttttggaa gagtgagaaa aaggttgtgg tgaacttttc
     8161 cttctgcacg attttttgta tatctgctga aaatgtctgc tcagaaaaca cccgcaattc
     8221 gctgattttt tgagtctgcc attctgccgc ttccctttcg atttcccgat ttgtttccat
     8281 gattcgctga taatcatcaa cgactagtaa actatttgaa gggaaataat ccaagatcgt
     8341 cacagattct tggtaaagaa gatctgagta gaattttgct tcttctgtcg ggacacctgc
     8401 ttgccagctt gaagtcagct gaccgaagta atcttgcaaa aactcgcggt ctgcttcttc
     8461 tgtggtcgca gcaagccgtt tttctagcat tttttgcatt tgctctattc catgttctag
     8521 atcctctggt ccgtagactt gttcactcgt tggggaaacc cagatttctt ccgttttttc
     8581 gattgagcgt tgtgtttctg cttcaaaata ccgcatagaa tctatctcta catcaaatag
     8641 ttctacacga acaggatagg ttgtcgttaa cgggtaaata tcgataatgc ttcctcgaat
     8701 actgaattct cccggttttg cgaccatctc ttctcgtaca tagcccatca aagtcaattg
     8761 gcgaggaaga ttttccacgt ctatctcatc accaatatgc caattcaatt gcgcctgtag
     8821 ccaagttttc ttattcggca gtcgtttgtg caaagatgcg actggcaaca aatagatacc
     8881 tgcttttttt tcttgaagtg cgttaagcgt tgcaactctc tcgcctcgcg cttctggaga
     8941 agaaaaggcc atttcagcag aaagcacttc atccactgga aataaatgga tctgatcttg
     9001 cggaatcaca tgttgcagat cttctgctaa ctgattcgca taatacaaat ttggcatcag
     9061 aataatcatg tttttatttg ttttcttaaa cgctcgagca aatagaagtg ttttcgctga
     9121 gcccgctaat cctgtgatta gttgtctagg cattttttct tctaaattgc tgacccattc
     9181 ttttacttgc ggatcttttc cgactaattg aataatatcc attccttttc ctccttcctc
     9241 aattgaatcg attcatcgca tccacgaacg aatgcccttc tatgagatat tcgactgctt
     9301 taacagattg acacatgctt tcttctatca aaggctgatt ttctttgctg aaaggactaa
     9361 gcacatgctg gactactgtt tttttccctt ctggcctgcc gattcccacc ttgattcgat
     9421 caaagacatt cgtattcaaa tgggaaataa tacttttgat accgttatgc ccgccggcac
     9481 ttcccttttg acggagccgg atttttccca ctgccagatc caaatcatcg tagattacga
     9541 ctaattcctc tggataaatc ccaaaataag tcattagtgg gccgactgca cgaccagatt
     9601 cattcatgaa tgtttgtggt ttgactaaaa ggatcttttc tccattatga aaaaattcgg
     9661 caacttctgc ttcgaacgga ttttttttga aagtagcttg atgctcgcgt gcaattttat
     9721 ccacgaccat aaagccgata ttgtgttttg tatattggta tttggtacct gggtttccta
     9781 acccgacgat cattttcatt tttgttttcc acctaattct aaaaagcagc acaaaaggct
     9841 ggactgctaa ttagcggtcc agtccatcga gcttttttct tttttatact tgtcaatgca
     9901 tagtataaca caaaacaagg cgtttcggct aaaagagaat cgttaataaa tgataaagaa
     9961 attaaaaatt tcttcaaagt ttaatgaaat aattgatata taaaaatagt gaaataacgc
    10021 acatgtaagc ttttacatat aatacaacct tgttatctgt tatattttat agccaaatag
    10081 cgtgacatgg ggcttgagaa atgttagcat aagcatggga aaaaacaaat caatggaagg
    10141 attgatacta aatgactgca gctgcagaaa aaaaagatca tcaaaaagtt attcttgttg
    10201 gagacggcgc cgtaggttct agctatgcct ttgctctagt tactcaaaat atcgctcaag
    10261 aagtaggtat tgtggatatc aatactgcta aaactgaagg agacgcaatt gacttgtctc
    10321 acgctttggc atttacttca cctaaaaaaa tctattctgc tacttacgca gacgcacatg
    10381 atgctgactt agttgttatt acagctggcg caccacaaaa accaggtgaa actcgtttag
    10441 acttagtaaa caaaaaccta aaaatcaacc gcgatgttgt cacacaaatc gttgaatcag
    10501 gatttaacgg tatcttttta gttgccgcta acccagtaga tattttgact tactcaactt
    10561 ggaaattctc aggtttccct aaagaacgcg taatcggttc aggtacctct cttgactcag
    10621 ctcgtttccg tcaagcactt gcggaattag ttgacgttga tgcacgtaac gtccatgctt
    10681 acatcttagg tgaacacgga gattcagaat tcccagtttg gtcacatgca aatgttgccg
    10741 gcttgcaaat ctatgaatgg gtaaaaaaca acccagaaat cgatgaagaa gctatggtta
    10801 acctattctt tggcgtacgt gacgctgctt acacaattat cgagaaaaaa ggcgctactt
    10861 tctatggtat cgcagtagca ttagcacgta tcactcgtgc catcttagat gatgaaaatg
    10921 ctgttcttcc tttatcagta ttcatgaatg gcgaatatgg attgaacgac atctacattg
    10981 gtgcacctgc tgttatcaac cgtcaaggta tccaaaaagt aatcgaaatt cctttaagtg
    11041 acagcgagca agaccgtatg gctgcttctg ctaagcaatt aaaagaaatt ctagatgaag
    11101 cttttgctaa actagacgct gaataatctt tgaacatgac ggaaaacatc tattcggtgt
    11161 tttccgtttt tttattttat cacccttatg gtttctaaat tttttcataa agtagagata
    11221 accttcgtct tttcctctaa attatgctat catgagaccg ttccacccta aacttttact
    11281 taaggagttg tcgatatgac aagatcttcg catcgaaaaa aatcgaaaca tgttgctttg
    11341 ctttcagttc ttggtgttct cttagtgctg atcagctttt atacctatcg cagtacatac
    11401 tacacaaagc attttttgcc gaatacagaa ataaatggaa taaatgtcag caatctaaca
    11461 gtcgaaaaag caaatgaaaa attaaaagaa gcttattctg ataaaaaact ggctattaaa
    11521 gaagatggaa aagtctggca ggaagtacca aaatccgagc tcggctacaa agatgatttc
    11581 acttcggagc tttctccgat cctaaatgaa caaaacggat ggacttgggg aatgacttat
    11641 gtctcagcgg cagaaaaaca agaaatagat cctgtcagtc aaaaccacca aaaactagac
    11701 actaccgttc agacattgac aactaagctg actgatttga acaaagaccg gacacctact
    11761 actgatgcaa cgatcgaaaa agcaggtgac tctttcgtta tcaagcctga agtcaatgga
    11821 aatacgatcg acgtcgatgc tgcggtcaaa caattaaaaa gtgcggtaaa ctctggtaaa
    11881 gatacaattg aattgactga attcaaagaa aaaccaaaag taacatctga agacagctca
    11941 ctgaaagaac aactcgcatc catgaatgct atcgcaaatg taaaagctac ttacagtata
    12001 aacggtgaga ctttccagat cccatcaagt gatattatga gttggctgac ctacaatgat
    12061 ggaaaagtcg atttagatac agaacaagta cgtcaatatg taacagattt aggcacaaag
    12121 tataacacaa gtacgaatga cacaaaattt aaaagtacaa aaagaggaga agttacggtc
    12181 ccagttggca cttacagttg gacgatccaa acagacagtg agacagaagc attgaaaaaa
    12241 gcgattctgg caggccaaga ttttactcgt tcacccattg tgcaaggtgg cacaaccgct
    12301 gatcatccat tgatcgaaga tacgtatatc gaagttgatc tggagaacca gcatatgtgg
    12361 tattacaaag acggaaaagt ggcccttgaa acagatatcg tttccggaaa gccaactaca
    12421 cctacacctg ctggagtctt ctatgtttgg aataaagaag aagatgcaac tttaaaagga
    12481 acaaatgacg atggaacacc ttacgagagt ccggtaaact actggatgcc gattgactgg
    12541 acaggtgttg gtatccacga ttcagactgg caacctgaat acggtggaga cttatggaaa
    12601 acccgcggtt cacacggctg catcaacacc ccaccaagtg taatgaagga attattcggg
    12661 atggtcgaaa aaggaacgcc tgtattggtc ttctaacaaa aataatatat aaaaagaaag
    12721 cacccgtacg attggtacag gtgctttctt tttattatct aacaatgtgc tacttacatt
    12781 tcagcagcag taaagacttc tgaaacatca tcatcgtctt ctagcttatc tactagacgt
    12841 tccaattgct ctttttgttc ctcatttaaa tcaatcgtcg tttgtggaat catcgtcaat
    12901 tctgcttgcg ccaaagtgaa ctctttttcc aactcatctc gtacagcagc aaaatcttct
    12961 ggtgcagtat aaatctcgaa tacttcagga gaagtttgca gatcttctgc ccccgcctct
    13021 aatacatcct caaacatcgt ctcttcatca acagtcaacc catcgcgttc aatagcgata
    13081 tatcctttgc ggtcaaacaa gtaactgact gaacccgttt ctcccagtga tccaccgttt
    13141 cgagtaaaag ctactcggac atttgtcgct gtccgattac ggttatctgt caatgcatgg
    13201 actaagattc ctactccacc cggaccatat ccttcatacg tgatttcatc gtaattcgtt
    13261 tcatctacgg cactcgtagc ctttttaatc gcccgttcca cattgtcgtt aggcatattt
    13321 gcagcttttg ctttgtccac aacaagccgc aaagctggat tcatagatgg atcaggaccg
    13381 cctgctttag ctgccatata aatttcacga gaaagtttct gaaagacttt ccctcgtttt
    13441 gcatcttggg cattttttct tccttggata ttatgccatt tactatgtcc tgacatgatt
    13501 tttcctccct tgttcttttg agaatacact tctattctag tactatttca caaaaaaaca
    13561 aagccacagc caatctataa acgcttcttg aatgccgcaa aaagcacaca taatatcaca
    13621 gcagtcagag caccgatcga atccaacatg acatcttgga acataggtgt tcgtccaccg
    13681 gtcatcatct gatggaattc gtccaaacct gcgtaccctg ttgctgaaag ccaagcaagg
    13741 atcgctgtca gccaccaaat ttttagcttg gggaaaacac ctaaaaacaa gctgcctccc
    13801 aaaataaaat aggtaccgaa atgtgcgccc ttccttaaaa agaactctaa aaatttggcg
    13861 tagcccagat ggtcgatact caccatactt ccaccataat taaaacgtat acccgctaat
    13921 gtgtctttaa atggctgatt aggcagccag ctttctaatc gcgaaacttg tgattgctgt
    13981 ccgtaagttt gagaagaact gtaaaataag attataacga ctatcagtgc gattactaaa
    14041 taaatatttc cgtttttcat ttgttttttt gaagaattca taagtcacct ctgtatttta
    14101 tcttagactt tttttgaaga aacaacaatc attttcatag agacttcttc cataaaaaag
    14161 tgctatactt aattttgtat tacaatatga agggagtttt ttcatgagtt attcattaaa
    14221 ttggaatcta gattctattt tttccggagg cagtcattct gatgctttga accaacggat
    14281 gaagcaattg gaagaccaaa tgaacgagta ttatcaccgc gtaacaaaat ggtcacctag
    14341 ttctgacaat gcagagcagt taaacgctat tcttcaattg caagaaacga ttacgaatgg
    14401 ttttacccaa tgcagcagct atattactgc attgctttct gccaatgtca atgattcaga
    14461 tgcaaaggtc ttatcaggca agctttacgc aatgctgcct cgtctgcaat cagccgaaac
    14521 tgtattgtca aagaaatttg cagaaatatc tgacaatgat tggaatcaga tgctttctca
    14581 atcttcgttt gaaacaattg cttttcgttt aaacgagatt cgtcgtgatg gctcacaatt
    14641 attatcagaa gctgaagaaa acattataaa tacgctttct ttagatggtc taaatgcttg
    14701 gagcagtcat tatgatacga ttgtagccag catttcgatt cccttcgaac aagatggaca
    14761 agtagtcgaa ttatctgctg gacaagcctt caataagatg atgggcgatc cagatccaaa
    14821 agtcagagaa acacttttcg cagcatggga agaagcctgg aaagaaaagg cacccctctt
    14881 cacggatact ttgaatcatt tagatggttt tagactttct gactatgaac ttcatggtgt
    14941 cacagatttt ctacaaaaac cattagagta caatcggttg aagaaagaaa cattgtctgt
    15001 catgtgggat acaatccaga aaaacaaaca accccttgtt aattacttga ctagaaaagc
    15061 taatcttttc ggtaaagaaa aaatggaatg gcaagatcaa gatgcaccta ttattttagg
    15121 cgatttgaaa gaaaaaacct ttagttttga cgaagcagca gcatttatta tcgaaaactt
    15181 taacaaattc agtccaaaaa tggctaaatt tgcccaatct gcttttgaaa agagctggat
    15241 cgaagcagaa gatcgtcctg ggaaacgtcc tggtggttat tgtactgagt taccagaaac
    15301 aaaagaatca aggatcttta tgacctacag taattctgtt aatgaggttg cgacacttgc
    15361 tcatgaacta ggacatgctt tccatagcag cacgatgtgg gatttacctt ctttaaatcg
    15421 cgaatatgcc atgaatgtcg cagaaacagc aagtacattc gcagaactga tcgtagccga
    15481 tgctacatta aaagtcgcaa aaacaaaaga agaaaaaatc aatttgctag atacaaaact
    15541 gcaaaatgct ttagctatgt tcatgaatat ccattcccga ttcatttttg aaaatcgatt
    15601 ctatgaagca cggcaagaag gattagtctc agaagataag atcacagaga tgatggttga
    15661 ggcgcaaaaa gaaggctatc aaggagcttt agctacttac catccatatt tctgggctgc
    15721 aaaattgcat ttctttattg atgacgtacc gttttataac ttcccataca catttggcta
    15781 cctctttagt ttgggcatct atgcttacgc caaccaaaag ggagcaaatt ttgaagaaga
    15841 atatatccat ttactacgcg atactgcctc aatgacgacg gaagatcttg cgaagaaaca
    15901 tttgaatgtc gatcttaccc aaccagattt ttggcaagca ggtatcgaca tggtgactga
    15961 agatatccaa acgttcatgg aattgacaga agaatttatc tgatttctat tttatcacaa
    16021 taaaaaaaga gttagtccag aatgggctaa ctctttttga ttaatcattc ttacatgctg
    16081 acagcaaagt caggggcaaa ccattgaact gaaccaactt ttacgctttc gcctggttga
    16141 ggagcatgga tatattgtcc tccgcctaaa gcaatcgcta cgtggtaagt tccgccttgt
    16201 gacccccaga ataacaagtc tccagctttt gcttgagaaa ctgaaatttt tgttccagct
    16261 gattcttgag gaactgtcca accaccgatg tcacgaccag ttacttgcat gtaaacatag
    16321 cgtgtgaacc ctgagcagtc aaagccactt ggatctttac cgccccaaac atatggagta
    16381 ccaatatatt tgtaagcttc tgctacgatt gctgcaccat tcacacttcc agaaggagct
    16441 ggagcaggtg ttacaggttt tggttgttca ggtgttggtg ttggcgttgg agccggagta
    16501 ctacttcctg tgccgtttcc tgtatcaaca ctttgatctg ttgatggtgt tgtaggcgca
    16561 ggtgttgttg attcttctgt tgttgaagat tcaggcactg ttgttgattc ttctgttgct
    16621 gaagattcag gcactgttgt tgattcttct gttgctgaag attcaggcgc tgttgttgat
    16681 tcttctgttg ctgaagattc aggcgctgtt gattcttctg ttgaagattc aggtgttgtt
    16741 gattcttctg ttgctgaaga ttgtgtagct gatgatgatt cttcagtcat tgaagattgt
    16801 gtagctgatg attcttctgt tgctgagctt tgtgcagtag atgaagcttg agtagcttgt
    16861 gcttcagcct ctgcttgttc gcgtgcttct ttttcagctt tttcttgtgc agcttgttga
    16921 cgagcttgtt ctgctagacg ttgttgttca cgaatacgag cttgctctgc ttcagcttct
    16981 gctttttgac ggttcaagtc agctttcttg tcttcagcag ttgcttgttc agcagctaat
    17041 gaagttttca gtacgttcaa atcagcttgt ttagataaaa gatcgccttt ttgagattcc
    17101 aaagcagctt ggttttcagc taattctttt agtttagcat cgttttcagc ttttttgtct
    17161 tctacagctt ttttatcttg tttttgttgt tccatcaagt cgttgtttgc ttttaccatc
    17221 gttgtcattg cttgaacgcg tccaatagca tctgctaatg aatcagcatt caatacagca
    17281 tcgatgtagt ttgagcttgt gttgcttact tgtgcttcgc gtgcttgttt ttggattgta
    17341 tcttcacgtt tttcaatacg ttcttgtaaa tctgcaatgt cttttactaa ttgtgcagat
    17401 tcttgacgca atgtatcttg ttttgctaat aggtcttgtg cttgtgtgtt gatttcagaa
    17461 acttggcttt ctaaggcatc gatttgtgat tgtgcatctg cttgttgatt tttcaagtct
    17521 gcaatctttt gatcttgctg ttgtatctga gaatcaaaat cgtccgccgc ggcagcgatc
    17581 ggtgatgcta cggcagtcaa ggtcattgaa cataccatta ctgctgatat taaactcttt
    17641 ttcactcttc attcctccga ctggcttaat ttaataaata tctaaatata tttaagtctt
    17701 tcttaacgat ataaaaattg taccatagca gtgtgtcaac gatataatag ttatgttaca
    17761 aaaatgtttc aaaacaactt ttaaaaatga caaaactgtt cgtttcagaa aatgatcatg
    17821 aaaaagccgc gttaagtcta gcatttctta agataacaac gattcttttt cgctttcgac
    17881 ctaaaaataa aaaaagaaat tattcatcac tgaataattt cttaaaggga aaaatgaata
    17941 atacaaatat aatcatgttc aacagcagtg taggtcctaa aaaccttgta ataaaatacg
    18001 tgttatttac tactgctaat ttaaaaatca actggaccac catcgtaaac aattcataac
    18061 ccgtgacaaa gatgatcata ctgaaaaatt cagtaaagat gttgacatgg ataacgtctt
    18121 tcattacata catcaaccat acaattaaag ggagagctac tgcatagata ccgataacgc
    18181 caatatagta agcgtcatag atagccccga gaacaatcgt ggtaatcagt aaataacgtt
    18241 tttcaaacgc catactgcaa cataataatg ccaatatcag aaagtgggca tttgacatat
    18301 aggtgccttt cgaccactcc cctagcattc ttgttaaatg accatctatc aacatcaaaa
    18361 agaacaaaac gattggcaga tagtacttca ttgtttcttt cttcaacatg gactaatcct
    18421 ccgctagccg ttggacaatc gtcacaactg acacgtcata catttctgca taaggtttca
    18481 cataaacttc ccgatccagc ccataactat ctggtttcgt tttaataacc gtaccgatcg
    18541 gcaagttagc tggagaattc cctccaagac cggatgtctg gacaacatcc ccttctttga
    18601 tatccgtatc accagttaat tgggtcacca ctaaggcatg gagcttttca tcatagtttt
    18661 taagcaaacc aaacgcttcg ccattagccg aagatacccg tactggaaaa tgattggagc
    18721 tttcattaga ggatgacagt aattcgattt tagacgaagc cgtattgacc tcgatcactc
    18781 ggccaatcaa tcctttttgc gacatgactg ccatatttgc ttcgataccg tctttagaac
    18841 ccttgtctac gatcagcatg tcctgccaac tgtcaggcga acgagtaatg acatttgccg
    18901 ttactttttc gtaactagtc agcgtctgat tcaactcaag ttcttttttc aacgcttcga
    18961 tttctctctc gtaattcttg ttttgctgtg aaacgtcatc atagctatcg attttttctt
    19021 ttagccgctc attttcggca taagttgaaa aaagattatg gacagaatct actccatttc
    19081 gtacccaccg tactggggca gagattgttt tatcaaccat tgcaacactg tcattaacga
    19141 tggattggaa gaaattgggc tttccttcat tcgctcgctg cgcagcggtc acacttagta
    19201 tcgtcaccac gatgataaca ataaccaagg taataataat atttttatta ggattgaact
    19261 tcttcacaaa gacacctcga attaattaga ctcttgtgtt caattttatc actaaatgcc
    19321 gtgaaacaaa aggtaagttg ctcctcttaa gctttttttt agcaaataat agattctgtc
    19381 atttttagcc tgaaagacaa atttttttat ttgtttatgg atcaatcttt gctttgaata
    19441 actgcaatca taccatctga aaaagaaaat tctgcggtat ctgaaagaaa ttcgttactt
    19501 gcataagatg tcggatattc tacttcttta ttttccaata catacttgct gccttttaat
    19561 gtaagattcg agacgggagt caagcagcaa taagctaaat acttcatatc tggtatctgt
    19621 cttactatat gctctccagg caaataataa gacaaataat tttgacaatc tctgataata
    19681 agctgatggg caaagggttt gaagcgaggt tccataccta accagagatt ggctaaaaga
    19741 tgatccaatc ttccgcccgt agctccaatc aaagtgactt ccgcttgcgg gtaacgttca
    19801 aatgttttta agatacctaa ctgtgtatct gtatcgtctt tttctgcagg ggaacgggta
    19861 tactctttgg cttttgcaaa tacttccttc tgctcttcgt tcgacaatga atcaaaatcg
    19921 cccactgcca gatctactgg gtagcctgct tgaagcaggt acagcgcacc acggtctatc
    19981 ccgacatagg catcgtattt ggacagcaga gactgcggcc agttttgcgg attacctcca
    20041 gcagctaata aaattttcat ttagtctaat gcctctttta atcgttcgat ttgtccgact
    20101 ggattttcgg ctccatagat atatgatcct gcaacgaaga catccgcccc tgcatcttta
    20161 cagatttttg ctgtttctgg aacgattccg ccatctactt cgattgcata agtccaattg
    20221 ttctctctac gtagatcagc gagttccgta attttttcta ctgtttcttc aataaagctt
    20281 tgtccgccga acccaggatt gacagtcatg actagtactt gatcagccaa aggcaaaaca
    20341 tgtttgatca tggagactgg tgttcctgga ttgataacta ctccagcttt aactcctaag
    20401 ctttttgtca tttgaagtgc ccgatgaata tgaggagttg cttcgacgtg gacagtaatg
    20461 atatctgctc cagcttttgc aaatgctgga atgtagtttt ctggatcaac gatcatcaga
    20521 tggacgtcta acggtaattt agtaacagga cggattgcct gaacaatatt tggccctaat
    20581 gtgatatttg ggacaaattg gccatccatt acatctacgt ggatgtaatc tgcgcctcct
    20641 ttttccacta actcaatgtc tctttgtaaa ttggcaaaat ctgcactcaa aatggatggt
    20701 gctatcttca ttcttgtttc ctcctatttt cgtttacctt ctttttttgc atacattggt
    20761 tttctttttt cgatttcttg taagaattgc aaataattgt cgtaacgtgt ctgtgcgata
    20821 ccacctgttt ctactctatg ttttacttcg cagccaggtt ctttagcatg catgcactct
    20881 ctaaatttgc aatggacaga agcttctaca aattcaggaa attgtctcgg cagttctcct
    20941 gcttcaattg tcaaaaagtc aatcgagcta aatcctggtg tatctgctac taacccgcca
    21001 tagataggaa tcagttccac atgacgtgtt gtatgccgtc ctcttccaag tgcatcagaa
    21061 atctctcctg ttgctaaatt cagttccggt gatatttgat tcagtagcgt cgattttcct
    21121 gctccagact gacccataaa gaccgatagc cgttctggga agtaacggat cagttctgtc
    21181 gtatcacccg tatgattggg tacaatcacc ggatagccta ttttagtata ggtttgcttg
    21241 atctcttcga ttttttctgg gtcgtctgac aagtcggtct ttgtcaaaaa aataatcggt
    21301 tcgatcgact cgtattccaa ggtaactaga aagcggtcaa gtagattata agaaaatgca
    21361 ggctctacga tacttgtgac aatgattcca agatcaacat ttgtgactgg tgggcgaact
    21421 aattgatttt ttctcggcaa gatttccaga agatagccgt ctgtttgatt ttcactctca
    21481 aagatcacct gatctccgac aagcggcgtg attttccgat tccggaaatt tcctcttcct
    21541 cttgtttgat aggtctctcc gtttgcgtat acatagtaaa aaccgctgat tgcttttctg
    21601 atttgtcctt tgataagtgc cacctccgtt tttgtttatt ttaccatacg aagaggaaag
    21661 taggtgttat tccatgcaaa cttctcaaag tatatggctt gaaaattgat caaaagagat
    21721 agaaacacaa aaaagagaat gcaacaaatc aatgtcgcat tcccttctga tatattttat
    21781 tcagcactta ttttgaactt tctgaggtgc tagctgatga acttgtggag ctggaagatt
    21841 cttcttttga agagttgctt gtgctactgg agctacttga gctgctagaa ctgtctgaag
    21901 agtctttttc agaactagaa gacgctggat ctggtccttt agagtaaatt acgttcacaa
    21961 ccattccttt ggtaattgtc gttcctgccc ctggtactgt acgaatcact ttatctttat
    22021 ccaccgtgtt tgaatattcg gaagtctcat gcccgatata ttctgcaccg acactagcta
    22081 aataactttg tgcttcgctt tttgtatagc cggagatatc attcaattta ggctctgttc
    22141 cttggcttac gacaaaattg atgtaagttt cttctgctac aacttcaccc gaggctggat
    22201 cctgactgat gatcgaacct gatggtacag tgtcgctata gttatagtca taagtagcac
    22261 taatattatt gatattgaat ccttgtgtaa caagccaatt acgaacagtt tcgtagtttt
    22321 ctcctacatg actactcaaa gtcactgttt ctggtccttt gctgactgtg aaagtaatcg
    22381 tgtctgtttt aggatcgact tctgtatctt ctgatggaga ttgcgaaatg atcattccgc
    22441 tttctacctc tgaactgaat tcctcttctt tttgaatcaa gtcagcagaa aaccctaatt
    22501 ctttcaaact tgcttcggca tcttcataac ttttaccact gtaatcatct aatttgattt
    22561 ttttcgtgcc gctactaata tacagagtga cttcgcgttt ttgcttgact gtcgttcccg
    22621 ctgccgggtc tgtcttcact acattacctt cttcgatttt gtcatctgca atttcttctg
    22681 tttttgttgc tacttctaat ttcgcttttg cgagtgtttc tctagcagaa gcttcagaaa
    22741 gatcagaaac atcaggaaca acaacttctc ctcgcccgcc agaagcaaag aatacagctg
    22801 ttccgattcc taaaacagca agaatagcta ataggatcag ccaccatttt ttctgtcctt
    22861 tctttttagg atcaggcaga acttcttcct cctctggcaa ggtttcgttt tctggcttgt
    22921 cttttggcag aggcatagat ttgaatgaat caggcattgg tgcatcttcc gtaaggggtg
    22981 tgataacttt tgtttcatta tccatcacat gaggctgcca tttttcttcg tttgcacgag
    23041 ctggtgataa tactgtagaa agatcatcag acatttcctc ggctgactta taacggtctg
    23101 caggttcttt agccgttgcc cgtaaaatca cgttttccag tgcttgtggc acatttggat
    23161 caagtgcttt tatagatgga agatcatctt gaaaatgttt taaagcaatt gttacagcag
    23221 attcaccatc aaacggtacg ctacccgtca acatctcata aaggatgatc cctaaagcat
    23281 aaatatctga ctgcttcgtt gccatacttc ctctggcctg ctcaggggac agataatgaa
    23341 cagatcccag catcgtattc gtctgcgtga tcgatgtttc agataaagcg atagcaatcc
    23401 cgaaatccgt gatttttaca acaccttcat tatcgatcag tacgttttgg ggtttcaaat
    23461 ctcggtggat gatttgatgg ttatgagcta aagaaatagc agaaaggatc tgctgcataa
    23521 tatttactgc tgtttcataa ggaacaggat aatgtgtttg gatatatcgt ttcaagtcca
    23581 ttcctttgac gtattccatc accagatact gcatgttgtc ttcttcacca acatcgtaaa
    23641 cactaacaat gtttggatga acgagttctg ttgctgctaa agcttctctc tgaaatcgtc
    23701 gaatcgcagt ctggtcattt tgaaagtcaa atcgtaagac tttaactgct acatcacgat
    23761 ccaagattaa atcgtgcgcc aaaaatacat ttgccatacc accgctaccg atatttccag
    23821 tgatttgata acggccattt aattttttgc ccaactcgat catacactgt cctccttata
    23881 ttcgataaga agaacggtga tattatccgc tccgcctgct tcattggcac gatcaatcag
    23941 ctcagttact ttatgagaca atgggccttc ttgattgatg atcgttccta tttcttcttc
    24001 agacagcatg ttcgtcaagc catctgaaca taacagcagt cgatctttta gttgccaaag
    24061 atgagtagaa acatctactt cgactgttcc aggcatgccg acagaacgtg ttaaaatatt
    24121 tttccttgga tggttcactg ccatttcttc gctgatttct cctgatttga ctagttcatt
    24181 gactaaagaa tgatcctccg tcaattgcat gattttatct tcacgaatca aataagctct
    24241 gctatcccca acattggcaa tcgtgaacca gtcatctgac agagcggccg ctacgattgt
    24301 tgttcccata ccgttatatt ccggctgttc ttgtccacgc tgataaatca gcatattttc
    24361 ttcttgaatc gattgaatga accattgggc gattttttca tcgtcaacag cttcttgttt
    24421 ttcccaagct tcgcctagat tcgttaccgc catctgactg gcaatgtctc cggcacgatg
    24481 accacccatc ccgtctgcta atacagcaag cttgagacct gaacggctgg taaatacact
    24541 ggcatagtct tgatttgtgt tacgtcgtct tcctacatct gattgatatt caatctgcat
    24601 tttttcacct cattactttt tgcgcaaaca acaaatgaaa aatccatctg tgtaaaactg
    24661 atgcggatac aaagtcaaca ttttctcttc gattgatgat tgtagcatag gattggtcaa
    24721 aacatcaatc ctctcaaaat cagggtgtct ttccaagaaa gcagaaacaa cgtcttggtt
    24781 ttcttctttt aagatcgtac aagtgctata agtcagaata ccggaagact ttaaagttgg
    24841 tgcgcagctt tctaaaattt ccaattggat ttttggtaaa ttcgctaatt catccggctg
    24901 ttttttataa cgaatatctg gttttcgccg aagcagccca agacctgaac aaggcgcgtc
    24961 tactaaaatc cgatcaaact gctctgcttc gaactgttta ctgacttctc tagcatccat
    25021 cttttgagcg tagacagcag aagagacacc tagacgtgct gcattatctt caatgagttt
    25081 gactttgtgt tcgtgtacat ctaaagcgac cacttctcca ccgcatgacg gatctaaaaa
    25141 agaagcaata tgcgtcgtct tacctccggg tgctgcacag gcatctaaga catgatgttc
    25201 aggctcgatt tgcatagacg gcgctacaag catcgaactt tcatcttgga tcgtcatgac
    25261 gccatcgtgg aacaatgagc tagaagctaa atgccctttt ttcgctacta taccgtatgg
    25321 actgacttgg cttttttcag ctgtgatctc ttcttcttgc aaaacagtga tggcctgatc
    25381 tctggaaagt cggcgtgtat ctactcgggc actagcatgg ctaggctgat atagcgataa
    25441 accgagcttt tcagtttctt cgtatccgat ttgatccacc agtcgtttcg tcatccatag
    25501 aggcaggctg attttgacag aaagtctttc gataggatca gcaatgcctt gcagatctgg
    25561 tgctccttga cgctgataat tcctcaagac accatttaca aatttccctg tcccaggatt
    25621 tccttttact tttgcgattt cgaccgcctc gtttaaaata gcatgtgcag ggactttatc
    25681 taaaaattct aattggtata cagaaagata aagaagcgtc ttgacccagt catccacttt
    25741 ttttgcttta gcgatcaatg gtgcaacgta aaattccaat agtcgctgat ggctaatcgt
    25801 accatagatc aattctgtaa ataaacccac atctttttga tttagctcgc tttgtttgat
    25861 catctcattc aacaaaagat tcgagtaggc gccacccttg ttcactcgct ctaatgtcag
    25921 taatgccata tatcgtactg attttttcat ttttttaggg atttttttca ttatttcacc
    25981 tgctgaccaa tctgaacttg ctgaccgctt ccgttaagga aatcatgtac ggcctgtttt
    26041 cctttaccag ctggctgtaa ttcattgatg gctaagacgg tctgtttacc gcaagcaatc
    26101 catagatttt ttttgtcttt ccggatgatc gtaccaggtg ctttgtctgt agtttctgcc
    26161 aatacttcaa ccgataataa tttccagcgt atgccctcat aggtcgtgaa tgcaattggc
    26221 caaggacgca ttccgcgtac ttggttgtca atttcttctg cagttttatg ccaatcgatt
    26281 gcttcttgtt cccgtgtgat gtttggcgaa aaagttgctt ctgactcgtc ttgaggtact
    26341 ggtttttgtc catccaaaat attgggcaat gtttctagca atagctttct tcctaaaagg
    26401 ctaagctttt caaacatcgt ccctacatca tcttgtttag tgattggtat gctttcttgc
    26461 gcatagattc cgcctgcatc catttttttg atcatttcca tgatcgttac accagtttct
    26521 ttctccccat tcatgatggc ataatgaaca ggcgcacctc cccgatattt aggaagtaaa
    26581 gaagcatgga cattgattgc ccctaatttt ggtacttgca ataacttttc aggcaagaat
    26641 tggccaaatg ccgcagtgac gatcacatca ggcaccaatt caatgatttc ttccatttct
    26701 ggcgaaccgg agattttctc tggctgcagc acacggatac cgtgcttcag agccgcttct
    26761 tttaccggtg tcggcgtaat tactcgtttc cgcccaactg gacggtcggg ctgtgttacc
    26821 actgcctgga tctcgtaacc tgattctatc agcccttcaa gaatcggcac agaaaaagca
    26881 ggtgtgccca taaatactag cttagtcatc aagatgttcc tccatatatt cttcaagttc
    26941 ttcttccggg atctgctgta tcattttctc aatgaacaag ataccgtcta agtgatcgat
    27001 ttcatgctgg aaggcacggg ctaaatagcc aaaagccgtc acttctatct cttctccgtc
    27061 tcgatcataa taacgcacgg tcacttcatc tgctcgcttg accgttccat agacatgcgg
    27121 aatactgaga caaccttcta cgtcaaggct ttcacctttt gcttcgatga tttctgggtt
    27181 aatcagttca aatttctcgc cttcatctac ttcgatcaca gcaattctct tgttttgtcc
    27241 tacttgagga gcagcgatcc ctattccgtc attcgcaatc attgtctcat ataaattgtc
    27301 taataaagcg attgtctcgt ccgtgattac atcgattggc tgagcagttc tttttaattt
    27361 atcattaggg tggatcaata taggatagcg cataatttgg ccctttctta aataaagttt
    27421 aatggttcgt tgtcaattgc cacaaaaaga ccttgtcgct gttctttctg gcttccattc
    27481 aaaatttcat ccaaaacatg aggaagattc ggttcatgtt tatatttgat gattagttga
    27541 taataatatc ggtttttcac tctcaaaatc atgctgggcg ttggccctaa tagcaagctt
    27601 tgagggctta acacttcttt tagctgattg gcaatctgaa agattttctt tgccgctact
    27661 tgttcttcag gatggctggc ggttattttc acagtaaagt aataaggagg atagcctcca
    27721 cgatgccgta catgcatttc ctgctgataa aactgctcat aattttgttt tttagctaat
    27781 tctatggcgt aatgttgagg attgaatgtt tggatcacga cttcgcctgc tttttccgct
    27841 cgccctgctc gtcctgaaac ttgggtaagc aattggaatg tatgctcact agaacgaaaa
    27901 tccggtaaat tcagggaggt atcagcattc aacaccccaa ctaatgtcac ttctggaaag
    27961 tccaaacctt tagcaatcat ttgtgtccct aataatatat ctgcttcttt tgcaccgaat
    28021 ttttgtaaaa tctgttcgtg tgcccctttt cttcgagtgg tatctacatc catacgcaaa
    28081 atcctagctt gcggataaag ttctcggagt tcctcttcta ctttttgagt acctgttcca
    28141 tagtagcgga ttttattgcc gccacaatta ggacagcgat tgggaatagg ttcttcatgt
    28201 ccgcaataat ggcagcgcat tgaacgtgta tccatatgca gtgtaagaga gatatcgcaa
    28261 ttaggacatg gaagaacaaa tccacagtcc ctacacatca caaaagacga atatcctcga
    28321 cgattgagca gtaggacagt ttgttctttt ttgtctaatc gattttgtat tttttcctgc
    28381 aaattagcag aaaaagtaga ggtccgatga ttctgaaact cttcacgcat atctacgatc
    28441 tcaatagctg gaagctgtgc agctgctttg gctcggtggt ttaactgcaa taactgataa
    28501 acgccttttt gagctcgtgc tcttgattct aaagagggcg tcgcgcttcc tagaactatt
    28561 gggcaatgat gatatttact gcgccaaatt gctagatctc tcgcatgata acgcggtgtc
    28621 tcatcttgtt tgtaacttga ttcatgttct tcatcgataa tgatcagccc gatatttttc
    28681 agcggggcaa aaatagcaga tcgtgcgcct actactactt gcgcttcttc ccgctcaatc
    28741 ttgcgccatt catcatattt ttcaccttgg gaaagtccgc tatgtaagac cgcaactgat
    28801 tctccgaagc gtcctttgaa tcgctcgacc atttgcggag tcaatgcgat ttcaggcact
    28861 aacataatag ccgttttatt ttctgacaaa acatcagcga ttgcctgaag atatacttct
    28921 gttttcccgc tgccagtgat tccctccagc aaaaagacct gatctttttg ctgctgacct
    28981 gctgtgatga tttgttcgac agcatttttt tgttctgcat tcagttccaa tgccgtcgtt
    29041 tggtcaaata ctcggtcttt ataagggtcg cgataacgtt ctgattctat aaactccagc
    29101 cagccctttt cttttccttg attcaagaca gcagtactga ttcctttatt tttgaaataa
    29161 gcaattggtt cttcttggct cagttcagat aaacacaaaa gaagttcttt ttgtttcttg
    29221 gcattttgcc gtaattttgc ccattcttct tcgatttgct tttcagttaa caaagaacgg
    29281 accaaacgaa tcgtttttac tttatttcgt gtatggactt catatttgat atctactaat
    29341 tgctcttggc gccagcgaat caattgagaa aggctgcctt cttcttctgc ttccttccag
    29401 gaaatcactt ctcgctgatc aaacaatacc tgaacttctg gtgtgtctgc caaaggatag
    29461 atcaatttat catattctgc tttcatcaca ctcggaagca tcgtttgtag acaagtgatt
    29521 ttgaatgcgt aagtaatttt tttcatataa tcagctaatg aaagtaattc ttcattgacc
    29581 accggagcaa ggtctagcaa acgaataact gccttcaaag atggattagc tgattcttct
    29641 gatttttgga tagccataac aaatccttgg acatgacgat tgccattacc aaaagggact
    29701 tctactcgca tcccgacttg caaagctgtt tccatttcag agggaaccaa ataagtgaat
    29761 ggctgatcgg tctgcatagt aggtacgtcc acaatgactt cagctgttaa tgtcatggtt
    29821 cgtttcctcc tttttttgtc ttttcttatc aagcgacaaa aagttccttg cctattttac
    29881 tagagatcga tggatttgtc ttatcttctg ccaaatccta acaaaaaatt aacagcagat
    29941 aagaaaaaaa agaactgtgt caaaaagttc agacacagcc ttttctacca gacaataagt
    30001 ggcgaatgtt tgaaaaggaa acagctgggt ttgcttttct ttttagatgt tcgctctatt
    30061 tttctttatt acattttttg ctcttcacgg atacgtgatt ccaattcgtg ttgttcacgc
    30121 tctttttgtg cttgacgttc ctcttcttcc atcttcaaac gttcacgttt taattcagga
    30181 tgtggatcat tgactacatt tcctgcatcg atttcttcta aagcttgacc gacgcttttt
    30241 actgaatcaa aagaatctag agtaggctgt gcacttgcat ccaattcatg cgcgcgttta
    30301 cttgctaaaa tcactagaga atattttgaa tttacacgat ctaatagtga gtcaattgaa
    30361 ggttttaaca tcataagact acatctcctt taacatttta atatatttac cgattacgcg
    30421 atctactcga aaatgttcac tggcaataat gtttttgatt cgttcaactg ctagcggcac
    30481 ttcatcattt acaacagcat agtcgtaaag agccatcatt tcgatttctt cttttgcaac
    30541 tttcattcgt tcttcgatta cttcatctgc atctgttcct cggcctacga tccgtgattt
    30601 taattccgct aaatctggcg gagtcaaaaa gatgaataca ccatcgggca ctttttcttt
    30661 gacttgtttt gctccttgga cttcaatctc caagaaaaca tcttttcctt catccaatgt
    30721 cttgttgata tatgacaaag gagttccata ataattcccg acatattctg cgtattccag
    30781 catctctcca gcttcgatca ttgattcaaa ctcttctttg cttcggaaga aataatcaac
    30841 gccttcgacc tccccttcac gcattttacg ggtagtcatt gagatcgaat actgaaaatc
    30901 attatcctca ctctcaaaaa ttgcttttcg tactgtgcct tttcctacac cggatggacc
    30961 agacaacacg attaataatc cacgctctga catgacgacg tcctttcaac tagtcttttt
    31021 gtacttatcg tttattttgc actttttttc tgcaactttc aaggttatct ttaattatag
    31081 ctagaaattg gtaaaaaagc taccctgtac caagaggtcg taaaaaagct gtcctgcatc
    31141 aagtaataag aacagccaaa cataaacagc ttctcatgtt tttcgtttgg ttggggttat
    31201 tatcgcagat gcaagctttt gaacgccgtg tataagaggt cgtgaagaag ccgattagtt
    31261 tcgagtatga agaaaaattt tgtgaaaata gtttctcaca tttttgcata aatttggctt
    31321 aatac
//
LOCUS       DASJST010000019        31015 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJST010000019 DASJST010000000
VERSION     DASJST010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.019"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(405..2480)
                     /locus_tag="U4B05_000659"
     CDS             complement(405..2480)
                     /locus_tag="U4B05_000659"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN2049814.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="U4B05_000660"
     CDS             complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="U4B05_000660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN2049815.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3783..4595)
                     /gene="recO"
                     /locus_tag="U4B05_000661"
     CDS             complement(3783..4595)
                     /gene="recO"
                     /locus_tag="U4B05_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN2049816.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4738..5637)
                     /gene="era"
                     /locus_tag="U4B05_000662"
     CDS             complement(4738..5637)
                     /gene="era"
                     /locus_tag="U4B05_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN2049817.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5652..6047)
                     /locus_tag="U4B05_000663"
     CDS             complement(5652..6047)
                     /locus_tag="U4B05_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN2049818.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="U4B05_000664"
     CDS             complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="U4B05_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN2049819.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6524..8716)
                     /locus_tag="U4B05_000665"
     CDS             complement(6524..8716)
                     /locus_tag="U4B05_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN2049820.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8740..9711)
                     /locus_tag="U4B05_000666"
     CDS             complement(8740..9711)
                     /locus_tag="U4B05_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN2049821.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10417..11796
                     /gene="brnQ"
                     /locus_tag="U4B05_000667"
     CDS             10417..11796
                     /gene="brnQ"
                     /locus_tag="U4B05_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2049822.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11952..12398)
                     /locus_tag="U4B05_000668"
     CDS             complement(11952..12398)
                     /locus_tag="U4B05_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN2049823.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12425..12601)
                     /locus_tag="U4B05_000669"
     CDS             complement(12425..12601)
                     /locus_tag="U4B05_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN2049824.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12763..13191)
                     /locus_tag="U4B05_000670"
     CDS             complement(12763..13191)
                     /locus_tag="U4B05_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2049825.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13283..14143
                     /locus_tag="U4B05_000671"
     CDS             13283..14143
                     /locus_tag="U4B05_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN2049826.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14227..14577)
                     /locus_tag="U4B05_000672"
     CDS             complement(14227..14577)
                     /locus_tag="U4B05_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2049827.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14593..16320)
                     /locus_tag="U4B05_000673"
     CDS             complement(14593..16320)
                     /locus_tag="U4B05_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN2049828.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="U4B05_000674"
     CDS             complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="U4B05_000674"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN2049829.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17604..19040)
                     /locus_tag="U4B05_000675"
     CDS             complement(17604..19040)
                     /locus_tag="U4B05_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2049830.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(19037..20440)
                     /locus_tag="U4B05_000676"
     CDS             complement(19037..20440)
                     /locus_tag="U4B05_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2049831.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20670..21572
                     /locus_tag="U4B05_000677"
     CDS             20670..21572
                     /locus_tag="U4B05_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2049832.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21613..23499)
                     /locus_tag="U4B05_000678"
     CDS             complement(21613..23499)
                     /locus_tag="U4B05_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2049833.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23596..24447)
                     /locus_tag="U4B05_000679"
     CDS             complement(23596..24447)
                     /locus_tag="U4B05_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2049834.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24905..26689)
                     /locus_tag="U4B05_000680"
     CDS             complement(24905..26689)
                     /locus_tag="U4B05_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049835.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26689..28440)
                     /locus_tag="U4B05_000681"
     CDS             complement(26689..28440)
                     /locus_tag="U4B05_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2049836.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28582..28806)
                     /locus_tag="U4B05_000682"
     CDS             complement(28582..28806)
                     /locus_tag="U4B05_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN2049837.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            29058..30644
                     /locus_tag="U4B05_000683"
     CDS             29058..30644
                     /locus_tag="U4B05_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN2049838.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8676 a         6650 c         5522 g        10167 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agcctatttt gtgcctattc tctggtttat ttacatatta gaaattattt tgtgatgatt
      421 tgatctaaac ttgctagcga caaagccatg gcagcaattt gacttaattg cgttaatcgg
      481 ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc
      541 agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt
      601 tttttcattg cctctactgc tttgtgcagt tctttctccg cttcattttc gaacagttca
      661 gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc
      721 aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct
      781 gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca
      841 tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc
      901 tgttgttgcc cttctgataa accaatgccg aaacgatccg tatctttact gataacatca
      961 ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga
     1021 acgacaccgt atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc
     1081 ccaactgcaa agaaagaaaa aatgctatct aatttatctg caatcgctaa aacagaacct
     1141 actgtagact gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct
     1201 gttgctacag caggtttctc gcctttcagc agagcatatt tttcgcccat gatcccttgt
     1261 agttcaggaa attcccctac catgttggtt actagatcaa atttatagat ttcagcagct
     1321 cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca
     1381 atgacttgta cccgctgcat tttctcatag atactaccga ttttttcatg gaaagtaaca
     1441 gtttttagtt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat
     1501 tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac attttccaat
     1561 tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcgttc
     1621 cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca
     1681 gaaagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg
     1741 acttcttcta gtaaatcttc gtctagatca ataacccagt gatttttttc ggctaattca
     1801 tttgcttgtg taacgatcat ttcttgtctt ttttgcggtg aagcaataac gaattgttca
     1861 ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga
     1921 tgtcctcgag aggctctgcc tgttttgaca tctaagatct caaacgggat tacttcgtca
     1981 tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa
     2041 tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct
     2101 ttcgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct
     2161 ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc
     2221 gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca
     2281 ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa
     2341 aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct
     2401 tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca
     2461 agtaataagt cttttgccat cttattcagc ctccttcatg ttttctggat ttgctgtgtt
     2521 tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc
     2581 acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacgg atactgcacc
     2641 gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat acgctggatg
     2701 aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa agttgcttag
     2761 tagcaagtct tggtcactta cttcaaaaga atatttggaa tgttcatatt ctggttgttt
     2821 gaagatctcg ccatatttca caccatcagc ccattccaga tcatagacac tttcaacttc
     2881 ttgaatatat gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca
     2941 aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc catcaagcca
     3001 tacttcccaa cccacaccgg cacaccccat cgatgggttc tcccagttgt cttcaacaaa
     3061 acgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc
     3121 ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg
     3181 gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc
     3241 attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc
     3301 tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa
     3361 tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt
     3421 ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga
     3481 ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc
     3541 agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact
     3601 ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt
     3661 tgcagttttc ttgtttatat aatagatcct ctcagtgaac gatctattta atctacggtt
     3721 aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc
     3781 atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat
     3841 acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca
     3901 tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag
     3961 tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg
     4021 cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg
     4081 ccagatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc
     4141 caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt
     4201 tctgcatcat ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc
     4261 gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa
     4321 ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt
     4381 aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc
     4441 aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg
     4501 ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat
     4561 aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct
     4621 ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca
     4681 acgtgcggat ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca
     4741 gtattcgtct tttcggtagc caaagtcttg cagatagact tttttgtctc gccagtcttt
     4801 ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt
     4861 tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt
     4921 ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg
     4981 cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa
     5041 gactttctca cggatcaatt cagaaacgat aaagtattca ggatgatcag tcacttgatc
     5101 atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc
     5161 gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga
     5221 ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat
     5281 cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc
     5341 gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta
     5401 agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc
     5461 gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt
     5521 ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga
     5581 ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata
     5641 cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca
     5701 atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt
     5761 ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta
     5821 ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc
     5881 cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc
     5941 ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac
     6001 atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat
     6061 ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt
     6121 gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta
     6181 agcttctgct tgttcagcag cacgttcaat agaaatcatg atgtctccta gtacacgagg
     6241 caaaggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag
     6301 agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg
     6361 gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata
     6421 agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt
     6481 tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt
     6541 cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc
     6601 tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag gtcagtccac
     6661 tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga
     6721 ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat
     6781 tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg
     6841 ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat
     6901 gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg
     6961 ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt
     7021 gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt ttgatcttgc
     7081 cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta
     7141 cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt
     7201 ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac
     7261 tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga
     7321 agagtgcacc agtgaacccg cagacaagca ttaaccaagt cgttgcatca ccaaagctca
     7381 ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg
     7441 cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag
     7501 agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa
     7561 tgaaaagtgc agatactgct tggaaaatac cagccatgat ccctgctctt cggttcaaga
     7621 aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt
     7681 gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca
     7741 catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa
     7801 tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatcgaag
     7861 tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat
     7921 ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg
     7981 cagattgttt taattcttct gtctttttct cattcaatac atcattgact acaatgcctt
     8041 gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa tactgaatct
     8101 gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct
     8161 gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt tcagtcattt
     8221 caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat
     8281 tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga
     8341 tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg atggtatcat
     8401 tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat
     8461 ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg
     8521 tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct acttggcctt
     8581 ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa
     8641 aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca
     8701 gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc
     8761 cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc
     8821 aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat gaacaagacc
     8881 acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga
     8941 ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc
     9001 gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta atggtgcgat
     9061 ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa
     9121 agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa
     9181 gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt gcacttcgcc
     9241 tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg
     9301 ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct
     9361 gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc
     9421 aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacga catctggtgt
     9481 acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa
     9541 ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat ggatggttgt
     9601 ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac
     9661 atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca
     9721 tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca
     9781 aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg ttaaaaaata
     9841 gctgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat gtaagttata
     9901 tgaaagtgga gaacaaaata ttccgtttca ttcagaggta ttctctaggt atttttcttg
     9961 tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta
    10021 ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac
    10081 gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc
    10141 atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg
    10201 gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt
    10261 accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt tatattttaa
    10321 ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca acgtttatat
    10381 cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca
    10441 tggaaacaac tattgatggt cagttcactt atcttcggga tgtttttcgg gtcaggtaat
    10501 ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag tgctggcgta
    10561 ggatttgcca tctcaggcgc tcttttccca ttactagcta tcttagctgt agtagtgaca
    10621 aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc
    10681 ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac agctgctact
    10741 gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt aggtatgttt
    10801 ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc cacacggttg
    10861 gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc aatcattttt
    10921 gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga tacttaccaa
    10981 acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc agtagccttg
    11041 cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa
    11101 gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt catgatctat
    11161 tttggtcttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc
    11221 ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc tagcttctta
    11281 ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca
    11341 caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact
    11401 ttcgtctctt ttgtcgttgc aaatgctggt ttggacaata tcatccaatg gtctttacca
    11461 gtattgatgc ttttgtatcc attgtcgctt gcgttgattt tgctttcctt aacagcaaaa
    11521 tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc tgttcccgcc
    11581 gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc
    11641 atgctcgaat tttatcatca tcacgttcct tttgctgcat tgggacttgg ctggatggtc
    11701 ccaacgttac ttgggtatgc tggcagctta ctcttctatt acgcctatcg cctatcaggg
    11761 tataaacaag aagcaaatga actaccagaa gaataaccag atcaaacaca atttaacata
    11821 agaaatatta aacaggaaaa aaacagctag atgaaagaat cctattcttt cgtctagcta
    11881 ttttttagat ctctttttta cgagcacaaa aaaactgcgg acaatgattg tccgcagttt
    11941 aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg gtttccatct
    12001 gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc tttcattgat
    12061 gaagcaccag tttgcgtgat tgcttcttga acaagttgtc gaatttcttc gtcggaaagt
    12121 tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc agctaaatcg
    12181 tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac
    12241 aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt
    12301 atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct
    12361 gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac
    12421 agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg
    12481 gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac
    12541 gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca
    12601 tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca
    12661 aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct
    12721 tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac aattttcaca
    12781 tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct gttcttcgaa
    12841 ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac agataaagtg
    12901 atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct ctccatttaa
    12961 atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat aaatcgtatc
    13021 gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag cggatacgta
    13081 gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc
    13141 gttatttttc aatgtttcta gtgattgttc gatcaacgtc tgttttttca tgacttattc
    13201 ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata aacataaaaa
    13261 aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt
    13321 catctccgac tctgctggag aaactgcgac aaaactagcg caagccacta tggcccaata
    13381 tcctagcgtt gagttcaatt tattccgcag aacatttgtg acagatgaga aaacattgat
    13441 gcaggcactg caagatgcac tagaagaaaa agctcttgtc ctgcacacat tgatcaatca
    13501 agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta gttttgatgt
    13561 gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca ttcggcaacc
    13621 tggcgccctt catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc
    13681 tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg tcgttttgct
    13741 aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa
    13801 agttgccaat ctacctttga tcccacaagc acatattccc aaacagcttt gggagatcga
    13861 tcctaaaaaa atcgtgggac tgacgaataa cccagatatt ttaaacaata tccgtaaaga
    13921 acggatgcgc tcttacggat taaatcctga tactgcttat tccgatatcg aaaaaatacg
    13981 agctgaactc gattttgcga atgatcttta tgaaaaattg ggctgcgtag taatcaatgt
    14041 cgcttcttta tctattgaag aaacagcttc gcttattttg aatgctttag acctagaaga
    14101 ccatagttat tatggaactg aaaccaccga agaaacagac taacaatttt tctttaaaaa
    14161 aactggactg tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct ctatttgcac
    14221 ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacgtgaga
    14281 atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga catggttttc
    14341 tcttaataaa cgcagttgat gcgatacagc agattgttcc atttccactg cttggatgat
    14401 tgctgtcaca ttttgttccc cgtctttcag atacagcaga attttgagac gagtggggtc
    14461 actcaacatt ttgaatagtt ggctgacttt ctctactttt gttttttgcg tgtctggcaa
    14521 tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag
    14581 tctcaagttt ttttattctt ctgtcatctt ttggataatc gtttcataag cggctacttt
    14641 ttcagctgcg tcttctgaag agtttccttt tacaccgata taaaatttga ttttcggctc
    14701 agttcctgaa ggacggatag cgatccagcc attttcttcc agcgtatact tcaatacgtc
    14761 agatggcggt gtcgttagtt tttctacgtt tccttcacgg tctgttctcg tcaactcttt
    14821 gaagtcttct gtttggatga cttttgctcc ggcaaattct gttggtgctt cttcacggaa
    14881 tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg tcacagaaat
    14941 cgttttttct gcgtagtagc cataggtttc aaaaatcgtt tgcaagccat catacaacgt
    15001 tttgccttgt tttttatagt aagctgctac ttctgctaat aagagcaatg cttggatcgc
    15061 gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga atccgaacat
    15121 aaaagtatgg gaatgatctt cttcaaattg ttgaattttt tcagcaatga atttgaatcc
    15181 tgtcaataca ttgaacatgg tagccccata gtgttccgca attgctgtag gtaattcgct
    15241 agaaacgatc gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg attggtgtgc
    15301 ttccaaaata tattgagcaa acagtgctcc gatttggttt ccggtcaata cttcatagga
    15361 gccatttggc aaacgaacgg ctgcacctaa acggtctgca tcaggatctg ttgcaatcag
    15421 caaatcagcg ccttctttct cgccaagtcg tatcgcatac tcaaaagcag agtgttcttc
    15481 tggatttggt gatttgactg tagtgaaatc tgggtctgcg acagcttgtt ctggaactag
    15541 tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt ttcctgttcc
    15601 gtgaagtggt gtgtacacaa gtttcaaatc tttccccatt gtttccacca attcatgatc
    15661 aatcgttact gctttgattt cttctagata agcggcatcc acttcttctc cgatgatcgt
    15721 gatcaatccg ctatgttcag cttcttcgtc agataatact tcaactttta acggattgct
    15781 gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt gtcccccatc
    15841 ttctccgtag actttatatc cgttgtaagc tgctggattg tgggatgcgg ttaccatgat
    15901 tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc tctggtgttg gacgtagact
    15961 ttcgaataca taagatggga tattgtgatg tgctaatgtt ttggctgctt ccatggcaaa
    16021 ttctggtgat tgatggcgtg agtcataagc gatagcgaca cctcggcgtt ttgtttcttc
    16081 gccttgtgta tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg tatagatgtt
    16141 cattcgattg atcccaggcc ctaagattcc ccgcatgcct gcagtaccga attccaacgg
    16201 tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta attgtttttt
    16261 taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt cccaagacat
    16321 gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca cttattagat
    16381 atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta tcaagcacgg
    16441 ttgcaagtat tcgcataaat cggattcagt agatttcacg cgaaaaaaaa gagtaaaaga
    16501 acaattaaac cgttcttcca ctctttttaa acttatgcag ttgtttcttc taaagattcc
    16561 aagtatttat acacttgttg tccagcgatc ccaccatcac caacagcagt agtgatttgt
    16621 cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc ctggtatatt cgtcttcatt
    16681 tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc ctgcttctct aaatggtgca
    16741 gttaatggat ccagtccaac atagatgaat actccgtctg ctgccaaatc aaactcattg
    16801 ccggttttga cattacgtgc tttcacacct gtcactacca tttcattccc aacgatttcg
    16861 tcaacgactg tatcccacat aaaggaaatt ttttcattcg caaatgcacg gtcttggatg
    16921 atcttttgtg cacgtaattc atctctacgg tggacgatga ctacttcaga agcaaattgt
    16981 gtcaaataga tggcttcttc tactgctgag tctcctcctc cgatcactac tagacgtttg
    17041 ttacggaaaa acgcaccatc acaaactgca cagtaagaaa cgccacgtcc agcaaattct
    17101 tcctcgccag gtacgcctaa tttacggtgg atacagcctg ttgcgataat gactgttttt
    17161 ccttcgtaag ttttatcttc acaaataact tctttgtggc tgccgtgatc cttgatatct
    17221 tgcacgattc cataagcatt ttctgtccca aatcttgaaa caccttcata catcttataa
    17281 gctagatcag gacccattat tgaatcgaaa ccaggatagt tttcgatttc tgccgtattg
    17341 ttcatttggc cgccaggggc gccttgttcg atcatcaata cggataagtt tgaacgtgaa
    17401 gcatataaag cagcagtcat cccagcagga cctgcgccaa taacgattac atcatacata
    17461 tgtagattct cctttactta atcaagtaag catactttac agccaatgcg aaaaaaagtc
    17521 tattgttctg cttgcgtttc tcgaatagct ttattatact cgaaaaatcc gctgaaaaac
    17581 aatagacttt tataaatcat tgatcaatca ttcgaaagat tttctccatt tgaatcaatt
    17641 acttttttat accagttgaa tgatttcttc ttcgaacggg ctaatgtgcc atttccttcg
    17701 ttgtcacgat cgacataaat gaatccgtac cttttcttca tctcacctgt accagctgat
    17761 actaaatcaa tacatcccca agtcgtatag cctaatagat cgactccatc taattctact
    17821 gcatctttca ttgcctggat atgtgctgcc agatagtcga ttcgatagtc atcttccaca
    17881 taaccattct catcgggcgt atcgactgca cctaagccgt tttctacaat aaatagtggt
    17941 ttttgataac gatcataaat atcattcatc gttattctca agcctaatgg atcaatttgc
    18001 caaccccatt cacttgcttc cagatatgga tttttgacag aagcaaagat atttcctgca
    18061 gtctgttcca agagttcgtt gtcagtagtc gaaactcgag aagaataata agaaaaggag
    18121 ataaaatcaa cagtatgttc tttcagcaat gcggcatccc caggttccat tttgatctga
    18181 atgccatttc gttccatttc tttcaatgca taagcaggat attctcctcg tgactggaca
    18241 tcaatgaaga aataattttc tcgatctgat tttctagaag cccaaacatc ttcaggattg
    18301 caagtgtatg gataatatgc tcctgctgcc agcatacagc ctactttgtt ctccggatct
    18361 acttcatgag caatcttcgt tgcgatggca cttgcaacca actcatgatg tgccgcttga
    18421 tatttgattt gttcttcatt ttctccttct tcaaaataca gccctgcgcc catgaatggt
    18481 gcatgaagga tcatattgat ctcattgaat gtcaaccaat atttgacaag tcctttatag
    18541 cgattgaaga tgactcggca cagattttca taaaaaccaa cgagttctct ggaacgccac
    18601 gcaccatatt ctttgatcaa atgcatcgga caatcaaaat gtgtaatcgt cacaagcggc
    18661 tcgatcccgt acttttgaca ttctttaaag acatcctcat aaaatttcaa accagattca
    18721 tttggctctt tttcatcgcc cattggaaaa atacgactcc aagcaatgga catccgataa
    18781 gtcttgaacc ccatttctgc aaataaagca atatcttcct tataatgatg atacatatcg
    18841 atcgcaattt ttgctggata aaaatgattt tcatcaaatt caaacatttt cttttttcca
    18901 gtaatgacag gaaaacggtc ttctccgatt ggcacaacat ctacgttcgc taacccacgt
    18961 ccgccttcat cataaccgcc ctcgcattga ttggctgctg tagctccgcc ccataaaaaa
    19021 tcttttctga atcccatcat acttcctcca ttacatgtgt attgcttgat aattctcttg
    19081 ccagttcatc ttggacttcg attccttctg cttttaaccg gctgattgtt tccttgaatt
    19141 gcggtaaata ctgctgatgt gcaataaaca actcattcat gatccgtttt gctgtctttc
    19201 ctgatcgtat aagcggattg atcgtaaacg cttgtaaagc tgttccataa tcaccagtca
    19261 cagctgcttc gatcgtcagc aattccatat tcttcattac ttgcaaccag cctctttcag
    19321 cagcaggaag ggaaccaaac gctacatttc gtgcgccttg tgcgccaaca taggcagtga
    19381 cttctactac acaatctgcc ggcagatcag gaacagctcc atcattcttt gtagatacca
    19441 cgatctgtgt ttctttattt ccatagatcg aagcaatcgt ttcacaggca gcatctgaat
    19501 aataagcacc acctcgtttt tgcaattgtt caggtttatg atccaattct ggatctttgt
    19561 ataattcaaa cagctctgct tctgtttctt tcacttgctg cgctcttgta ccaatcgttt
    19621 gatattcttc taaagcatgt tccagcattt cttcttcacg gtagtaatag cggtggtagc
    19681 cacaagggat catgttcatt tgttttaatt gttctttgaa aaacgggata tcaaagatat
    19741 ttttcggcaa tccattgtct tcatcgtata aacgatcaat caattctgcc gtgcggtcat
    19801 ttcctttatt atctgctact ttgtgccaat ggaagtgatt tagaccggca aacttgtaga
    19861 tcagctcttc ttttttcttg ccaagcattg ttggttctgt catcatcgcc attactggca
    19921 cattgcataa tcctatcact ttatcccatt tgccatatct gataactgct tctgtcacca
    19981 tcccgcttgg gttcgcgaaa ttgatcagcc aagcatttgg acataaacgc ttcatgtctt
    20041 caacgattgc caaaaccact ggaacggtac gaaaagcttt gaatatcccg ccagcaccat
    20101 ttgtttcttg cccaatcatc ccataataac tagggatccg ctcgtctttg attcgtgcat
    20161 ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc atctttcaat gcttcttccc
    20221 gatccagagt tgcatagatt ttgacttcat acggagaagc atcccacata cgctgagaca
    20281 tctgactgac gatctcaagc ttttcttttc cttcttctac atctacgagc cagatttctc
    20341 gtatcggtaa ttcctcatat cttttgataa atccttccat caactcgggg gtgtaactgc
    20401 tgcctccacc aatcgtagcg attttgattc cttttgccat gttctcactc ctttactttt
    20461 tacactttta ttatcttttt tgtaaacaaa acaaacaata gaaattcgtt tctttccagt
    20521 aacatttaca aaagttagtg ttttctactg gatttttgta aatatcttgt caaaaaatgt
    20581 ctttattaag ctatataaca taaatccgtt tacaaatcat tttcaaaaaa gttataattt
    20641 acgtaaaaaa acaagaagaa ggtatctaca tgttattacg tgaaaaaatc aaagaaatct
    20701 ccttttctcc ttcagaaaat gaagtgattc agtttctctt aagcgattct ccagcacttg
    20761 gcgaaatgac catccaagag attgctgagc gatgttatgt ccacccgtcc acactcatcc
    20821 gtgtagcaaa aaaaatgggt tttgatggct gggtcagttt tcgtaatgct tttttagctg
    20881 aaacagccta tcttgaaggg aattttcaag acgtagatgc gaatcttcct ttttccacca
    20941 cagacggtat tatgacgatc gcaaataaaa tggcttcttt agaacagatg acgattcaag
    21001 atacactttc tctcattcat catgaccaac tgcaaaaagc caaacaacta atgcttcaag
    21061 caaaaaaaat cgtgattttc gccagtaact cgaatacttt gatttctcaa gatttcatgc
    21121 tgaaaatgaa gcgcatcaaa atggatgtca cgattgtcac cacttttggt gaatcgttct
    21181 atgaagcgta caattgcacg agtgatatgt gcgggattct gatttcttat acgggtgaaa
    21241 acaatgtgat ccttcgtacc gcagaaatat tgaaacaaaa tcatgttcca attttagctt
    21301 tgaccagtat cggtgattca acgctatctt caatgagtac agccgctttg cgacttacga
    21361 cacgcgaacg actgtattcg aaaattggta attttacgat caatacatcg atttgttatc
    21421 ttctagacgt gttatatagt tgtgtttttg cagaaaatta tcaacagaat ttagtccatc
    21481 taatttctgt aggacaaaaa tttgataaac gccctatcag ctcagccatc atgaaagagc
    21541 ctttcaattt tgaaaataaa gaagtggact aaaaagaata attaagccta gtccattctt
    21601 tcacctaacc gttcaggtca gtgcggtgat aagtgcatcg tctggcccga ccgtctcccc
    21661 ttgattttcc aaaatatcca aataatccgt gtaattggtg acgatcacag gtgtttctaa
    21721 actgtatcct tcctcttcga ttttcttttt gtcgaagcgg agcaacagat cgccttgttt
    21781 cactctttct ccttgctgca ccaagctttc aaaatatttc ccttccaact gaacagtatc
    21841 caatccgaca tgaatcagca attccagacc tgcttcagaa accattccga ttgcatgttt
    21901 cgtcgggaac agggtcatga ctgttccgtc aaaaggggca cgtacttctc ctttttcagg
    21961 gtagatcaag attcctcgac ctagtgttcc ttgtgcaaaa gctgcatcct ttgctgcatt
    22021 cagtggagcg atttgtcctt gtatcggtgt ttttacaatt tctttacctg atcttttctg
    22081 tctatcatct gtttcacctg cttcctcctt ccaaaagaaa aaagtcagcg caaaggatac
    22141 caccatcgca atggcgattc caatcaatac attaatcaca tccgttaaat caccatctgg
    22201 tctaatatag ttgggaattg taaagatgcc taatccacct ggattccaag aagttaaatt
    22261 cattaatcca ctatacgcgc cccctaacgc accaccgaca caggtaaaaa taaatatttt
    22321 tttacgtggc aatgtaaacc cataaatagc tggctctgta atccccacaa taccagaaac
    22381 aatggctggt attgcgattg ctttgtcttt tgggtttcta agcttaaagt agagtgcagt
    22441 aagtgctgca gtttgtccaa aagacactac tgcaacaggt gtcatgatat tttgccaacc
    22501 attcacagaa aattgcatca atcccattgg tacgattgcc caatggagac caaacatcac
    22561 tagaatttgc cagaagaagc ctacgattgc tccataaata actggcgaaa gtgctaaaag
    22621 tgtttcaaat ccagccatta aaatatcagt tgcgaatgtg aatgctggtc caacgagtaa
    22681 aaaacctaag ggcattgtaa taagaacggt gaagaatgga acgaagaaat tcgcgatcat
    22741 ctcaggtact aactttttaa ttggtttctg aatttggcta gctaacaaca caataaagat
    22801 aatgggaatg acagaagaag cgtagttacg cgcaatccag ggaatgccga atactgtttg
    22861 ataaataggt gcttcaaaaa tagtgccaga aaatatggta gtcagtggtg cttctgctgc
    22921 accactcaaa gcttccagcg acaagctagg attcattaat gctgccccaa gcaacatccc
    22981 tacgaattca tttactttaa atttacgggc agctgttaaa ccgataataa caggcataaa
    23041 ttggaataca gaatcaccga tattatcaaa cataacaaat gtgccagaac cgcggtcaaa
    23101 tgcgcctaag aagacaagta atgctgtcag tccacgaatc atcccaccag cagctagaat
    23161 agcaaggaat ggctggaaac agcccgatat tccatcaata agcatatcaa acggtctcat
    23221 tttttctgtt gaaacagcct catcaaaagt aaggtttcct gcttttataa cctcttcaaa
    23281 cacgactggc acatgattcc cgatgacaac ttggtactgt cctcccgctc gcattaccgt
    23341 cacaacaccg tccatatttt tcaagatctc atcatttgct ttttcttcgt tttttaattt
    23401 gaaacgaagc cttgtgatac aatgtgtcaa accattgata ttttccttcc cgccgacatt
    23461 ttctacaatt ttgctcgcta attcttgata ttttcccatt tctattccct ccaatttatt
    23521 agtggaaggt ttagccaact gaatgtcact acccttattt catgcgttct tgttaattta
    23581 tcctcctttt tttatttatt tacaacgatt ctcatgcact aaacgcgcaa tatggatcgt
    23641 taaatacatt ttttcgtccg tggacatctg gtagttgaat atctcggcta aaaagtctgc
    23701 tattttctca acgcaaccaa acgtatcctt atattttttt tgaataacct ggtacaattc
    23761 ctcagaaact tctcccactt ctgatttttc tccatctagg acgcgtgtca gaaaaaaacg
    23821 taggtgtgtg acgaatcgaa aaaaatggac tgaatcttca tcaaatgtaa tcttatagta
    23881 atagcggacg atatttagaa tatcctgtat tgttgtagta aacaggtagg tattgtcttg
    23941 ttggctttga atgccttgtg cattgactaa atgcaatgcg ataaatccgg cttcatcttc
    24001 tgataacttg atgtggtatt tatccgagat tttcttcact gtttttttcc cgatggcata
    24061 ctctttagga aatagttgtt tgatatcata cagcaataca tttggtacaa atattcccat
    24121 tttgtgacgt tcgatagccg tatacaaatg atccgtcaat gcaatataca ccgaatcatt
    24181 caactggact tgtagagcct cttttgtata ttcaatgatt tcacaagaaa tttctaagta
    24241 ctcaagagga atcgcttgtg ccaattcttg gaactgttgt gaaagtccgt catttgtcaa
    24301 ctgaaatttt ttgtcgatgc atgagtcaaa aatcgggtca cccactttct ttttgaatgc
    24361 caaacctttt cccatcacga tcatttcctg atttctttca tccctggtta ttacgacgtt
    24421 gttattcagt actttctcaa tcagcatgtt ttactccttt ccatgatata tagggctgta
    24481 tccaaaacaa aaagaaaaac ccaaaaagcc gtccatggaa ccatgggaga ctcatcaggt
    24541 ttagcttgca tcaggcaatc actatccaag ttgtatcgtt ttctttatat tatcgaaagc
    24601 aagcactttt gtcaacgctt tcttagcggc tattgggcac ttctgactag ctgtcgtgat
    24661 tttttttaaa tctggtaatt acgacactaa cgctcacaga tgaagaatct gggaagctct
    24721 gttttatttt cactctagcg cttaggtcaa aatgctacta tgctactgtc ccaacttcta
    24781 atatacggtg ttccagtacc agcttatcgg tgataagcca aatttatgca aaaatctgga
    24841 aagccttttt tacaattttt ttcttatcac ctaaagctga acggagccct gtcacaacca
    24901 ctttttacac accactgctt tgcagtttgt acatatctgc atacaaacca ttttgcgcta
    24961 gaagtgtttc atgattccca cgttctacta tatgaccttt gtctaaaact aaaatcaatt
    25021 ctgcatcccg aatcgtggac agccgatggg caatcgcaat cgttgtccgt ccttgtcgca
    25081 tttttgtcaa tccttcttgg atcaatcctt ctgtttcagt atcgatattt gctgtcgctt
    25141 catccaatac tagtatttta ggatctgtca cgattgttcg cgcaaaagtg atcagctgtc
    25201 gttgtccgct agaatagctg gcaccgtgtt cgatcacttt tgagtgatat ccttttggta
    25261 attcatcgat gaacttgttt gcttgaacaa actcagctgc atggcgaatc tgttcgtctg
    25321 tgatagttgg attcatcatc cggatatttc ctgcgatatc tccataaaac aagaaggcat
    25381 cttgcaagac caatcccatt ttcttacgta actcttccat tggatattct cgaatgtcat
    25441 gatcatcaat caagatctgg ccttcataaa attcgtaaaa ccgcatcaat acgttgatga
    25501 tcgagctttt tccgcttccg gtgtgcccca ctaatgcaac ggtctctccc ggattagcga
    25561 caaaagagat attgttcaat acattgtgct ctccatcata agaaaagctt acattgcgaa
    25621 actcgatctt acctgaagta atcgcttgat ctgctccagg attttgttgc ggtgcatatt
    25681 cttctgtatc catgattttc aaaatacgac taccggcaac cattccatct gtaaaggtgc
    25741 tcaaaaagtc catcatttga gacattggat taaaaaatcc ctggacatag gtcgtaaatg
    25801 catagatcag tccaacttct acgggagaat gcagcgcatc gatcccaaac aaagtcaacg
    25861 ccaacgcaat cgccaatgca tatagaaaat tgatgatcgg acttaacaga atcgaatttg
    25921 ttttgatcat cgcatagcgc gtgtctaaat actcgttgtt cacttgttca aattctttag
    25981 ctaaacgagc ttcttggcga aactgctgga taatacgcat gccggaaatc gattcattca
    26041 atttcgtgtt cagctgactt aatttttccc gcatattccg gtaaatacga gaactgaatt
    26101 tctggtaata ccagatgacg attcctaaaa tcggcaagaa caccaaagtc cacaaggcaa
    26161 tttctccatt gatttgaaac atggcaatga aggaagaaac gactgcaaag atcccagtca
    26221 ataccatcaa aaagacatac caaaattcaa atagcgtttc tgtgtcattt gtcactcgag
    26281 aaacagtcga tcctgccggt gtctgatcaa aataacgcat acctagtgta tgcagctttt
    26341 caaataatct tacccgaaca tactgatagg tcttcaatga agccatggaa tacaaatacc
    26401 attggaaaaa ccaaatgata cttttgacta tgactccaaa caagtacata ccagcaaaaa
    26461 acaaaataac ttgtgtcgta gccgactgtt tagccagatg atcatccata aaagtttgga
    26521 tgatccttgg caataggaca ttgatcacag acagggcaaa cgcaaataag atagcaacga
    26581 tgaacgttct tctaaaaggt ttggcgaact tcatcagtcg tttgatgatc ttcttctgtt
    26641 ctttaagtgg aatcgatttt gtccattccg attgataatt ttcatccatc aggcctcgcc
    26701 tccttctatc ttcgcttcta atttctgttt ctcccacatt cgttcatacc agccgcctaa
    26761 tgcaagcagc tcatgatggg ttcctcgttc aatgattttc ccttgatcga caacaatgat
    26821 ttcttttgca tgcatcacgc tgcttaaacg gtgagctgca atgatggttg ttttctcttt
    26881 tcttatttct tttaaattat gcaagatagc ttcttctgtt ttggcatcta ctgcagaaag
    26941 tgcatcatct aagatcagca attcaggatt tttgatcaat gctcgagcaa tcgaaatcct
    27001 ttgtttctgt ccgccagata aagagactcc acgttctcca actaaggtat cataaccttt
    27061 tggtaattcc ttgatttcat tgttgataaa tgctaaagaa gccgcatgct ctacttcttc
    27121 ttctgaaagt tttggattag aaaaacggat attatctcga atggtcatcg aaaacaagaa
    27181 gtgatcttga ggcacatagc cgattgatcc taataaagca ttcaatgaat attctttgat
    27241 cgaacatccg ccaaattgaa tttttccttg ataatcatcg tattcacgca tcagtaattt
    27301 gatgattgtc gtttttcccg caccagtttt cccaacaata cctaatgttt ctccttcttg
    27361 gatcgcaaat ttgatttgtt ctaatgctgg tgattcttct ttcggatagt taaaagagga
    27421 aatagctaca ttcagctcgc cttttgccgg agtctgcact gcacccagtt gttccacgat
    27481 atgcgtcttt tcatgcaaaa gttcattgac gcgatcataa gaagcatttc ctctttccaa
    27541 tacgttgaac aatcgaccaa tcgcaaacat cggccaaaca agcataccga tatagctaat
    27601 aaatgagacc atctgcccga ttgagatcgt gccttccaag acatactttc cacctaaaat
    27661 aattgttgca acataagaca gaccgataat caaggtaatg aatggatcaa ataaggagtc
    27721 taaaaaattg actcgtttgt ttttaataat tgcatcttca atcttttctt caaaatccgc
    27781 gagatcttct ttttcttgtc caaatgtttt aatgactttg atcccagtaa tactttcttg
    27841 tgtcttatca ttgatactag aaaaagcttc ttgcgaatca cgaaaagcat cgtggagttt
    27901 tgaccccagc actcgtgaag taacggctag taaaggcaga ggaatcagcg caatcagtgt
    27961 taagcgccaa tcaataaaca aaatcatcgc aatgatcgtt gttccgcccg tgatcactga
    28021 atctgcaaag gtcaagatac cagctcctgc tacattttgg attgcattca aatcattcgt
    28081 tgcatgagcc atcaaatctc cagtccgata tttttggtaa aagagattgt ccatttctgt
    28141 aaaatgtttg aatagctgct gccgaagttc tttctccaac cgagctgcac ttccccaaat
    28201 attcgtccgc cagatataac gaaaaagata ttgtaagaga gctgcaagga tcaataataa
    28261 gatccaccag gaaatgatct ttattgataa atttttatcg gcgatctcat caatgatgat
    28321 cccaatcact tttggaggag ctaactgtac caaggccact gcgaataggg agaaaatccc
    28381 aattaaataa tgtttctttt cctgtcgaaa aaaccaacct aattttctaa aaatcgacat
    28441 tttactcctc ctttgtcttt atcaagagca taggattgtc tgtgcttgtc ttttttgctg
    28501 aatagtctga atagtctaac aaattacgaa ataaaaaacg gactgaaaaa atcagcccgc
    28561 aaaaagtctt tgggtcagaa cctatttttt cccttggttc ttcatttgct gcatcatctg
    28621 gcgaattttc ttctcagatg gtttttgccc cattgacatc atcattgatc gcaacatgtc
    28681 ctcattaacg ggaggatttt tttctaaata gtctttcata tatttacgcg ctaggaaaaa
    28741 gccacctact gcgccgatta gcaaagcaat aatagcaata agtacgacaa tacctgttga
    28801 taccatataa atctctcctt tctatttccg aattgctctc aatgatttat tttactaaag
    28861 tttattgaaa aaagaaaggc gtattcttta gtttcatgca aaaaatagtg gtttttttag
    28921 agaagaacaa aatttctttc attaaccagc aagacacgcc acgaaaaaaa aattaggcat
    28981 accaaatctt tttctttaat tttttctttc tgaggtgtag aatgtaatcg tagataaata
    29041 ggaaaggtgg ttttttcttg aataatcaat ccaacaaaca atttcataag catcgactta
    29101 ttgaacatac aaatggttta tctttagctg aagtaaatgg aacagtggat gtacccaagg
    29161 gaaaaagttt ttttaaaaca cttcttgctt actctggacc aggtgcatta gtagcagttg
    29221 gttatatgga tccaggtaac tggtccactt cgatcactgg tggacaaaat ttccaatatt
    29281 tattgatgtc agttattttg atgtctagtc tgattgctat gcttttacaa tatatggcag
    29341 caaaattagg aatcgtgacc caaatggatt tggcccaagc aatccgagca cggacaagta
    29401 agacattggg tgtcatttta tggatcttga ctgaattagc cattatggca acagacatcg
    29461 cagaagtcat tggtgcagca atcgccctct acctgctttt tgatattcca ttggtaattg
    29521 ccgtatttat cactgtgtta gatgttttcg tccttttatt gctgacaaaa gtcgggtttc
    29581 gtaaaatcga agctttggtt gtctgcttga ttttcgtcat tttattcgtt ttcgtctatc
    29641 aagttggctt gtctaaccct gattggcaca gtttgtttgc cggatttatc cctaacagtc
    29701 acacattctc agactcaccg gtcatcggcg gcgaaactcc gctgactgga gctttaggga
    29761 ttattggtgc aacagtcatg ccgcataact tatatcttca ttcagcagtt tctcaaactc
    29821 ggaagatcga tcaccaagac gaagaagatg tagcacaagc ggtacgcttt tctacttggg
    29881 attctaatat ccagttgtca atggcttttc tagtcaatgc actgctattg atcatgggtg
    29941 tggctgtatt caaaaccggt tctgtccaag atccttcctt ttttggtctg tatgaagctt
    30001 tatctgatcc atcagctatg agcaatggta tcttgattgc tgtagcaaaa tctggtatct
    30061 tgtctacttt atttgctgta gcacttcttg cttctggcca gaactctact attactggta
    30121 cgttgaccgg tcaagtcatc atggaaggct tcatccatat gcgtatccca atttggctaa
    30181 gaagactagt cacacgattg ctttctgtga ttcctgtatt gatttgtgtt ttgtttacaa
    30241 gtacaaaagg aacgatcgaa gaacataccg ctttgaataa cttgatgaat gaatcccaag
    30301 tttttctggc ttttgcattg ccattctcga tgattccttt actgatgatg accaatagcc
    30361 aagcggagat ggggagccga ttcaaaaatt catttgttgt aaaactactt ggctggttct
    30421 ctgtgattag tctgacttac ttgaatttac gaggattacc aggtcaaatc gaaagtttct
    30481 tcggcgagca agccacctca gctcaactag cattggctga tcaaatcgct tatgtaatca
    30541 ttgttcttgt ccttctcctg ttggcatgga cgattggtga tttatataaa ggaaacaaaa
    30601 aatatgaaca gcgtttgcag gaaacgatag aagaaatcga gtagaaactg attcttttac
    30661 acaaaggagg ttgtgtcaga agtgggcagc ttcaagaaat aagaaggaat ttaagaaaat
    30721 tgcttttcaa atttttgtga aatttcggct tatttccgaa gaagttactt ctggaacacc
    30781 gtgtatcaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa
    30841 acagcttttt atgtttttaa cttggttgga cttattaccg cagatgcaag cttttgagca
    30901 ccgtttattc ggaaaaagga actgcgacaa gacttttgtc acagttccct ttcctacgat
    30961 tagtcaagta aaaggtgtaa gaaaaatagc tcataaacga aagcgacaaa atcaa
//
LOCUS       DASJST010000020        30595 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJST010000020 DASJST010000000
VERSION     DASJST010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.020"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            363..731
                     /locus_tag="U4B05_000684"
     CDS             363..731
                     /locus_tag="U4B05_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN2049839.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="U4B05_000685"
     CDS             911..1852
                     /locus_tag="U4B05_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN2049840.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="U4B05_000686"
     CDS             1921..2652
                     /locus_tag="U4B05_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN2049841.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="U4B05_000687"
     CDS             2796..4976
                     /locus_tag="U4B05_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN2049842.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="U4B05_000688"
     CDS             4996..5478
                     /locus_tag="U4B05_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN2049843.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="U4B05_000689"
     CDS             5494..6255
                     /locus_tag="U4B05_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2049844.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="U4B05_000690"
     CDS             6386..7720
                     /locus_tag="U4B05_000690"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN2049845.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="U4B05_000691"
     CDS             8027..8512
                     /locus_tag="U4B05_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN2049846.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="U4B05_000692"
     CDS             8505..9188
                     /locus_tag="U4B05_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN2049847.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="U4B05_000693"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="U4B05_000693"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN2049848.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="U4B05_000694"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="U4B05_000694"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN2049849.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="U4B05_000695"
     CDS             12804..13433
                     /locus_tag="U4B05_000695"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN2049850.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="U4B05_000696"
     CDS             13485..13817
                     /locus_tag="U4B05_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2049851.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="U4B05_000697"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="U4B05_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN2049852.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="U4B05_000698"
     CDS             14643..16016
                     /locus_tag="U4B05_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2049853.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="U4B05_000699"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="U4B05_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN2049854.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="U4B05_000700"
     CDS             17071..17511
                     /locus_tag="U4B05_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN2049855.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="U4B05_000701"
     CDS             17655..18356
                     /locus_tag="U4B05_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN2049856.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="U4B05_000702"
     CDS             18353..19474
                     /locus_tag="U4B05_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN2049857.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="U4B05_000703"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="U4B05_000703"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN2049858.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="U4B05_000704"
     CDS             20853..21761
                     /locus_tag="U4B05_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN2049859.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="U4B05_000705"
     CDS             21796..22068
                     /locus_tag="U4B05_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN2049860.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="U4B05_000706"
     CDS             22079..23125
                     /locus_tag="U4B05_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN2049861.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="U4B05_000707"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="U4B05_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN2049862.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26822)
                     /locus_tag="U4B05_000708"
     CDS             complement(26136..26822)
                     /locus_tag="U4B05_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049863.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26800..27294)
                     /locus_tag="U4B05_000709"
     CDS             complement(26800..27294)
                     /locus_tag="U4B05_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2049864.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="U4B05_000710"
     CDS             complement(27314..28534)
                     /locus_tag="U4B05_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049865.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="U4B05_000711"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="U4B05_000711"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN2049866.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10251 a         5446 c         6594 g         8304 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg ttttt
//
LOCUS       DASJST010000021        28583 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJST010000021 DASJST010000000
VERSION     DASJST010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.021"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..613)
                     /locus_tag="U4B05_000712"
     CDS             complement(<1..613)
                     /locus_tag="U4B05_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2049867.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRL"
     gene            complement(632..1672)
                     /locus_tag="U4B05_000713"
     CDS             complement(632..1672)
                     /locus_tag="U4B05_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2049868.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(1688..3079)
                     /locus_tag="U4B05_000714"
     CDS             complement(1688..3079)
                     /locus_tag="U4B05_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2049869.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            3457..4440
                     /locus_tag="U4B05_000715"
     CDS             3457..4440
                     /locus_tag="U4B05_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN2049870.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(4808..6946)
                     /locus_tag="U4B05_000716"
     CDS             complement(4808..6946)
                     /locus_tag="U4B05_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2049871.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(6985..8571)
                     /locus_tag="U4B05_000717"
     CDS             complement(6985..8571)
                     /locus_tag="U4B05_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2049872.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(8561..9781)
                     /locus_tag="U4B05_000718"
     CDS             complement(8561..9781)
                     /locus_tag="U4B05_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049873.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(9793..10599)
                     /locus_tag="U4B05_000719"
     CDS             complement(9793..10599)
                     /locus_tag="U4B05_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049874.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(10809..12089)
                     /locus_tag="U4B05_000720"
     CDS             complement(10809..12089)
                     /locus_tag="U4B05_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN2049875.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(12133..14166)
                     /locus_tag="U4B05_000721"
     CDS             complement(12133..14166)
                     /locus_tag="U4B05_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049876.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(14200..15051)
                     /gene="rfbD"
                     /locus_tag="U4B05_000722"
     CDS             complement(14200..15051)
                     /gene="rfbD"
                     /locus_tag="U4B05_000722"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN2049877.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(15149..16177)
                     /gene="rfbB"
                     /locus_tag="U4B05_000723"
     CDS             complement(15149..16177)
                     /gene="rfbB"
                     /locus_tag="U4B05_000723"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN2049878.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(16199..16771)
                     /gene="rfbC"
                     /locus_tag="U4B05_000724"
     CDS             complement(16199..16771)
                     /gene="rfbC"
                     /locus_tag="U4B05_000724"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN2049879.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(16785..17651)
                     /gene="rfbA"
                     /locus_tag="U4B05_000725"
     CDS             complement(16785..17651)
                     /gene="rfbA"
                     /locus_tag="U4B05_000725"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN2049880.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(17751..18485)
                     /locus_tag="U4B05_000726"
     CDS             complement(17751..18485)
                     /locus_tag="U4B05_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2049881.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(18463..19290)
                     /locus_tag="U4B05_000727"
     CDS             complement(18463..19290)
                     /locus_tag="U4B05_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2049882.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(19290..20090)
                     /locus_tag="U4B05_000728"
     CDS             complement(19290..20090)
                     /locus_tag="U4B05_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2049883.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(20083..21222)
                     /locus_tag="U4B05_000729"
     CDS             complement(20083..21222)
                     /locus_tag="U4B05_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN2049884.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(21427..22620)
                     /locus_tag="U4B05_000730"
     CDS             complement(21427..22620)
                     /locus_tag="U4B05_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2049885.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(22699..22905)
                     /locus_tag="U4B05_000731"
     CDS             complement(22699..22905)
                     /locus_tag="U4B05_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2049886.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(22898..24040)
                     /locus_tag="U4B05_000732"
     CDS             complement(22898..24040)
                     /locus_tag="U4B05_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2049887.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            24552..25187
                     /locus_tag="U4B05_000733"
     CDS             24552..25187
                     /locus_tag="U4B05_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2049888.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            25529..26164
                     /locus_tag="U4B05_000734"
     CDS             25529..26164
                     /locus_tag="U4B05_000734"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2049889.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            26271..27509
                     /locus_tag="U4B05_000735"
     CDS             26271..27509
                     /locus_tag="U4B05_000735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2049890.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            27736..28155
                     /locus_tag="U4B05_000736"
     CDS             27736..28155
                     /locus_tag="U4B05_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2049891.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            28160..28399
                     /locus_tag="U4B05_000737"
     CDS             28160..28399
                     /locus_tag="U4B05_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2049892.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         8743 a         5649 c         4888 g         9303 t
ORIGIN      
        1 ctaatcgtcc aatgtttccc acaacaaaag tactcattga gatatccaat tcttttctct
       61 tattaacctt agcttctgaa tttttactat aaagctctaa atcaatacca ttatgaacta
      121 tctcaaaggg gtggttttga aacagacttt caccagcttc ttgactaacg gcaaggtagt
      181 ctgttgcatt ttgtaacaac aatttggtta aaaatttacg caatacattc attacaatat
      241 tttgagattt tccatcttca gttgcatggc tatgagcaat tctctgtctg attccattct
      301 ttttggctaa ccataaatta attcccccgc caaaataaac ctgattgtga actatagaaa
      361 aagattcttt ttttagtaac ttattcactt ctattaaata tttaaaaata tttttcttag
      421 gattaggtac aacaaaaata cggcctccta atctttcaat ctctgaatga tagtcttgaa
      481 catctgcata atcttcataa atcaaaaaat caaattgata gtttgtacga tcaattttac
      541 gatataaatt catcatgaaa gactctgctc cacctaaacc cattcgtccc tgaaaatgca
      601 aaattctttt cattatattt tcctccatta actaatatac tcgcttcaaa gcagccatta
      661 accctggcat tttcacttca acgaagcttc caacttttac agcagataaa aatagtggag
      721 ctggcatatt gtaaagcaac tttgttattt tttttgctga agaatccgtc ttagcaattt
      781 gcttggcttc tcggatcact ggatcactca atatctgctt tgcttcgtct ttcttctctt
      841 ttgaagttct tgcagaggta ctataaatag aaaaaataac tgaagttgtc ccttttaaaa
      901 gaagcgccgc cataccgtct ttattaatat cgtctgttac agtatttgct aatgaataga
      961 attctttaaa tgtttcgaac ttgttaggtc ggtattgatt agtgatcccc ttcccacctt
     1021 caaattcata aatatattct acttttgggt tcatgatcac ttgatgacag tattcataat
     1081 aatgtaagtt aaatattcga tcctcacaac tattataagt tttaaataga atttggttat
     1141 ttttcagaat atcgctacga tagcatttgt tccatacaac atacatcaac tgttcattca
     1201 ttaactgata catgtctttt ataaactctt cgtgagttag atagtgacat tctgtaacta
     1261 agttttcttt ttcggaaacg attcttccct cgtccattgt acgaaaaaca aacgaactaa
     1321 caacaagatc cgcttttgga ctcttttcta gcaaatgatc ataatctgac aagatcgttt
     1381 ttactggcgt gtcatctgca tcgaagaata agagatattc tccttttgac tgtcttattc
     1441 cttcgtttct tgctaatccg ggcccttgat tggtcgtagt aatcaattca aaacgttcat
     1501 cattttcaat cagttcttga atgatttcct gtgtttgatc tgttgatcca tcatttacta
     1561 aaacgacttc gtactcttca taatccaatt cttttagaat atcaatcgtt cgaccaatag
     1621 ttgcttctgc attgtatact ggtacgatta tgctgaattt cggattgttc atttttacgc
     1681 ctcccaacta gcgaaagatt ttttcattcg gaaaagcaat ttctgccaat gagatcacat
     1741 tttcgtcttc ttcaagtcct tgagaggaca ttttatcaaa taggatcttg atggtttgca
     1801 gcaagatctt taaatcaagc atcaatgaat attttttgat ataaagtaaa tcaaaacgta
     1861 atttactgtt gtaatcagag acatattttc catagacttg tgcataccca gtgataccag
     1921 cacgaacatt atgccgtaag tagtaataag ggttttctgc attgaactga tcgacgaaga
     1981 aaggacgttc aggacgtggt cctacgatcg acatatcgcc tttcagcaca ttgaagattt
     2041 gtggtaattc atcaaagcgg acagctcgaa taaatttacc tacaggcgtt actcgtgcat
     2101 cattgctctt agctaaaact ggtccagatt ttgcttcggc tgtggcggtc atcgagcgga
     2161 atttatagat tgaaaattcc ttttgatttt ttgtgatccg cacttgttta tagatgattg
     2221 gtcccggtga tgtcactttg actaaaattg ctgtgaataa cataaatggt gaagcaataa
     2281 ctaataaaac aagtgaaacg atgatatcaa acaaacgttt gaatagttca tattcaggtt
     2341 ggattctaaa tccagatgct tcaataatac tttcatcttc gaaattcata atatttggat
     2401 tgatcaatgt caagttttca aatgttgtat tcaaaaacaa ttttttttca tttttagtaa
     2461 ctaattgata gattttgatt ttttcagttt cttcgatatg agaagcaaga tagacaatat
     2521 cgatgtcttt aatcatattt ttgatgtttg cataatagtt atcaagaatg actgcttcga
     2581 ctttatgttt gttgtttttc tttgaaccaa aattttggat tgctggtgcg acatcctttt
     2641 ctaacccaat aatgacgacc tttttatctc gacttacgcg taaataaagt ttatatacga
     2701 gaatacgata gatacttaag aaaagcgtac cgagaacaaa tgaaagtaag ataacaagac
     2761 gtggaaaagc aaaccagcgg cccgcaaaag tcactaccat gatccctagt gtcatcaata
     2821 cctgaccgat gactgtgaca aacacgatgt cactgatcat tcgattataa aaaacatatg
     2881 cacccaacaa aatgttcaac gcaataaaaa tcactgaaat aataattgct gagtgttgga
     2941 aagtttctaa atttcgtaat gggatctctc cacgaaactt cagtaagaaa ctactatata
     3001 ttgctgcatt gtatacaata acatccatta atataatgaa tatccgtcgt gcagcactcc
     3061 attctccatt tttattcata tatacactcc taatcacaaa ataatatctc tattatctta
     3121 ccatagtcac ctttaaaaac ctagtttata agcaaattgt aatggaattt aaaaatgtct
     3181 aataaaaagc tttatacacc tctaatcaag acaaggactc taaattacaa atctccagat
     3241 gaatcgattt tcactaatgt tggtaaatta aagaagtcga agtatttgct gcttttttta
     3301 tccactcata aagtgaacct ttatattttt cagagaatcc atcttcccaa agcgctgtct
     3361 agtccataag aatacctgta ttacgtgaag aatcgaaaaa attgaaatcg atttttaatg
     3421 acattatgct tatagaagaa agtgaggtaa tatcttatga ataaacaaga gttgcatgat
     3481 tttattggtt ttcgacaacc aaatatatgt caaattgtcg catataagga tggggtagaa
     3541 gtttatacag atgaatggaa tggttataaa aaagacgata cttgtcatat catgtcagca
     3601 acgaaaagta ttgtatcttt gctgattggc attgctttag agcaaggatt aatcaaaagt
     3661 atcgatgata aagtattaga ttattttcca gagtataaag tgaaacgcgg ggagaaaact
     3721 atttacgcta ttacaatcaa gcacttgcta acaatgaaag caccctacaa atgtaaaggc
     3781 gatccatgga caaaagtctg ttcaagtgaa gattggacaa aaacatcact agatttattg
     3841 ggaggaagaa aaggtataac cggcgaattc aactatcaaa ctgtctgttt acacatatta
     3901 actggcttgt tgtatcagac aagtaagcta acaactgttg attttgccaa taagtatcta
     3961 tttgatccta tagggatacc aaaccatgtc aattattatg ctgaaacagc tgaagaacat
     4021 aagcagttta caatggataa gtctcctaaa accaatgtct ggttttgtga tccactaaat
     4081 atcgggacgg caggatatgg tttgtgttta tcggcaagtg atctggcaaa aattggtgta
     4141 ttatgcctaa ataaagggat gtataaagga aaacaaatcg taccacccat gtggataaac
     4201 gagatgttca aacctagaac cagtgcttta gaagacttac aaaatatgag atatggctat
     4261 ttatggtggg tattagattc tgagaacgaa atatattcag caattggtaa tagtggaaat
     4321 gttctttata ttgacgctaa gaacaatctg gttatctcca tcacttctta ttttaaaccc
     4381 atgataattg atagagtggc ttttgtagag gaatacctta aaccatttat tttaaaatag
     4441 cctatattaa aataaaatat cgattctttg acagctaggg ttcgtgaaac tttggctatg
     4501 attttgtatc tattggcgct ctgagcatga ataagagcgt gggacaaaag taagaaacac
     4561 ttttgtctca ttctttaaaa acgaataaac ggtgggagca gaagcaactt cttcttaaat
     4621 aagccgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttctcgga
     4681 gttaaacact tctgtcccaa cctcgactta tttttttgag tcggcacctt ctgccttttt
     4741 ttgacaaaaa actataaaaa gccgttatgt gcgagcgaat cacaacataa cagcttttta
     4801 ttatagttta atctggtctc aacgaaaagt cgccgattcc ttcttttgtt aaattatcat
     4861 tgtaaaatgg atcatgcgca atatatgctg gccatttatc ttgcattttt ttgatttcac
     4921 cagcaaaacg tctttgtttt tctggagtat cttcataacc tcttgattta gattcgaaat
     4981 ggtacaattc tgcttgatgt gcatagacat tgtaacgacc cagttcataa actttcaagc
     5041 acaagtcaac atcgttgaat gctacttcta atgtttcatc aaacccgtta acagcattga
     5101 agtcagctgc cttgaccatc atacaagctg cagtgactgc taagtaattg acgtcaatga
     5161 ccaatcggcc gaaataacca caatgtgttc gatcatagtt attcaatgca tgccctgcaa
     5221 caccaccgat tcctaataat actcctgcat gctgcgttgt atcatctgga taaaacagtt
     5281 ttgctcctac acagccgatt cgatcaaact gtgcataaga aaccatcacc gtcatccaat
     5341 ctggctcaat cacttctgta tcgttattca agaataaaaa gtatttgccg tttgcttttt
     5401 ctgctgctaa attgttaatt cgagaataat tgaatggaat gtcaatcaat tctacgataa
     5461 agcggtcttt cagctgatgc ttgtattctg caaataattc ttgcattttt ggatctgtac
     5521 ttccgttatc cgcaataatg atttcataat ttgaataaga cgttttttcg atgatcgaat
     5581 ccacacaagt ttttagatct tcatagccat tcttcgttgg aatgatcaca ctgaccaaat
     5641 cctcttgcaa gacgtcataa gcaatctcat aaaatccaga aattctgcca ggacgtacgc
     5701 ttcctttgat gtttcttcta gacagggcat ctgtcaatgc tttcactccg gaatcataga
     5761 tataactttt ggcttcgcca ttacttgcag ttgatcctgg gatcgtccgc caatgataca
     5821 agactcggtc aatgtggtaa atatgatctg cagggatttg ttctgtcaca cgaaggacca
     5881 aatcataatc ttgagaacct tcatagcctt tacggaaacc gccaagttct ttaacaatgc
     5941 ttgttcgata tacgcctaag tgagagatat aattatttcc catcaatgta tcaggcgacc
     6001 agtctgcttt gaaatgcgga tcaaagcgat tgccatctgc atcgatttta tcttcgtcac
     6061 tgtagatcac atccagttct ggatgtacgt ttagcacttt tgccacttca tataatgcaa
     6121 atggcggtaa ttcatcatca ttatctaaga gtgctataaa ttctccttct gctatttcca
     6181 atgcagaatt agttgccaga ctgatgtgtc cattttcttt tcggaaaacg actttgattc
     6241 tatcatcttt tgcttgatag gattccagac actttcggat agctggatca gtagaagcat
     6301 catcactgat acatagttcc caatgatcat acgtttgatc cagtaccgaa tcaatgcatt
     6361 tttcgagcca tttgatttca acgttataga caggcataag tatggaaatc aatggacggt
     6421 acgcaaatcc ttgtgcttct ttcctttgct ctttcaaatc tggtttctca tgacgactga
     6481 tccactctgc atagtcgatc gatcctggct tcaattccaa tttgacccga cggatcgctt
     6541 tttttacgcc atgagtcctt gcataattta tccctttttt cagtaatctt ttttttcttt
     6601 cccatgaaga ttcaatgccg tcatcataag ggtatttacc gtttaatttc acagctatgc
     6661 cattttgatg tctcgctgtt tggaagtcta agatggcttt tcctctcatt ttccctgata
     6721 aacgtacctt aaaaccgctt tgggtcttaa catcaagatt atataaatga ttgatatcca
     6781 atcttacgac atgtgttgct tcagcaatca cattttcttt ttctaccttt atcgtaggga
     6841 tttctttggt cacttcatca attgcccagc ccacaataag caaatcgcca gtggcccgtt
     6901 cacgagtgat ttgatcgata aatacggcaa tttctttttt ttgcacgatt cgtttactcc
     6961 ttatctttat aagttaatca ttatttacat ttcaatgatc catgattggc cattttgagc
     7021 tactgcttcc actaagaatg aacgttcttg ctcgcaaagg attcgtaact caaatcccgc
     7081 ttctgttcct tttgcgagct gttctgcttg attgacttct tctcgtgaaa caggaattgc
     7141 ttcgaaaaat ggcgattggt cagctgataa cttataggac aagggctggc gatccgaatt
     7201 agcgattccc catccgatga tttttaatat ttttgtttct gcatcccaga ttttctcgtc
     7261 aatacatata gaaagttctt tttcttggat ttttttcgaa gtttctttct tacgtcgtcc
     7321 tagtctatgt tttattgtcc attcatcact ttccaacaat gtgcaatagc gatttgtggt
     7381 tgtttttaac gtatggtcta gtcttgtatt ttcctcaaga agatgatgat tttctctttc
     7441 taattcgtca tttttctcag aaagctgctt tgtgacttca gccatgggac gaatcgtttc
     7501 caacaattct ttcattgttg gtgtcaattc cccgataaag cgatagtgac aatggatagt
     7561 cacttctttc ccagaaatat ccgttagaac gaactctggt aattctttcc catcaaacaa
     7621 ataacaatca tttactgttt tgactacagc attcgaatcg ataaatgtta gtttttctcc
     7681 ggcagcttct gcgctgaatt cgataaagct aggttgttgc gcgaagcgaa acaccattct
     7741 ttctgttgtt tctgatacgg ggaaacttag tgtctcattt tcccctgtga aattattgaa
     7801 tggtatttgt tggattgttt catcttgttt cgtttgctgg gttacttcta acacttttac
     7861 ataattagaa ttttgtggct ctgcaatgga actttgtgct actggatgtt tcatcaatga
     7921 gaaaaagtat tggtagactt caccaaatgg tcgcattttg aaatcatagc gtacagcttc
     7981 cggcaattct tcataagttg attttaattc tgtatcacct acagctaagt acaaataatc
     8041 ttcaatatta atgaataatc ccgctttttc aaaaactttt tggaagccat catgagtata
     8101 aaaagaatta tgggtttcat ctaggagacc ataagaagcc cacggcaatt ggttattgaa
     8161 caaatcgatc atgacagaat tatgcgccaa gttagggaat gtaatcagga tctctccttc
     8221 ttcattcaaa aattctctga ctttcttcaa tgtttttcca ggatctacca aatgttctaa
     8281 aacatccgca aacaagacgt aatcaaatgt ctgtccagca taatagtttg cccactcaaa
     8341 actttcgata tcaccataaa atccatcttg agcaaattcg ctgacaaaat cgaacaattc
     8401 cttatctaac tcaacaatgc tcacttcaca ttgttttgct ccgattaaat gtttcgttaa
     8461 tcggccgttt ccgggaccaa actctaaaac tttacttcct ggtttgatct gtgcggcaat
     8521 ttttcccaca ctgctttgtt catctaggtc catttcaaaa tcatatttca ttgctatgag
     8581 tcccttcttc ccatttatgg tctaagacca cgatgccttc tccgatataa gaaccgctga
     8641 caaacaagtt catgtacttt gtcttatata ccaaaggaac cgttgcattt tcttcaaaga
     8701 ccgcaacgtc aaaataatat tcaccagcta acaaacctaa cttttcatac tccagataaa
     8761 agatattctc tccttttttc caaggaatct tgataccatc tagtagagta ttaagtccgc
     8821 atacataaaa attgtcgact gttcgtagcg caattcctaa aaccggattt ttgattgtat
     8881 catcttttac ttcataagtt actttaacgt atagtttttc tccttgttcc agcatatcca
     8941 ccggattttt gtctttatcc aacaattccg ctttcgtcac gtcgacgata tctggagcgg
     9001 cgaaccgttc tacttggttt ttattccgat ccactgtttt aactgatttt ttctttaaaa
     9061 agttttcata attcgtcgtt acttccatcg tatcgccata ttcgacgact tctccgtttt
     9121 tcaaccaata ggaacgatca caaaaacgac gaatcgaact gatatcatga gaaacgaata
     9181 aaatcgtctt tcccgagttc ttgatttctg taaatttttc catacatttt agttgaaatt
     9241 caagatcgcc tactgccaat gcttcatcca caatcaaaat atcgggattc acattgattg
     9301 ctacagcaaa agctagacga acgaacatcc cgcttgaata agtttttaca ggttgataaa
     9361 tatggtctcc gatgtcagca aattcaatga tatcatcgac catttcatcg acttcttctc
     9421 ttgaaaatcc tagaaccatt ccatttaaat agatattttc atagccagaa tactcaggat
     9481 tgaatcctga tcctagttct agcaaagctg agatttttcc ttcgatttcc atcgtgccgg
     9541 atgttggtgt cagtactccc gtaatgattt tcaacattgt agattttccc gatccgtttt
     9601 ctcccacgaa accgatcatc tcaccttttt ttatttctac attcacgttt ttcaacgcat
     9661 aaaatacatc atgataggat ttcttaaaag gattgagggc ttcacgaaat cgatccgaag
     9721 gttttgcgta catattataa gattttgtga tatcttttag tctaattgca tattctgtca
     9781 tagctattct ccttacagca catctgaaaa atgtggtttc aatctacgga acactgcaga
     9841 gccgattacg aacattacta acgtgaaaac ccagaaatac aaagtatatg ttccatgctc
     9901 ccagaaccaa gcttcactta aaaatgactc acgataacct tgaacaacat agtacagcgg
     9961 atttaatttc aatatgctta caagacctga atcaaactga ttagggctcc ataatatcgg
    10021 taatgtccac atgatcaact gcagcacgat cgtaattaat tgcatgatat caggtaaaaa
    10081 tggttggatc gatgccgtca gccaagtcaa tcctgtcaag aaagcacaca tgcaaaataa
    10141 ataataaatc agctgtaaag acattgctga aggatatata ccatttataa gcgcgacagc
    10201 tatgccaatc agaatgaaga ataagtgggt gtacagattg gataggattt tcgtcgtggg
    10261 caagatacga atattgaata cgactttctt tactaaataa gtatattccc gaaaaacaac
    10321 cgtagttgat gcaaatgcat ctgagaagaa gaaccaggga atcatcccgg taactaaata
    10381 tacgataaat gggtattttg catcgatcat ttgtgctttc agtccaacac caaataccag
    10441 ccaataaatc aatacagtaa atagcggatt gataaacgcc cataagattc ctaaaaacga
    10501 tcctgcatat cttgatttga agtcattgat cgaaaactgt agcaataatt ttctgttctg
    10561 ataaatgtcc ttcataaata aatacaaatc tttaaacatt tgtgttcctc caagcgattt
    10621 tcagtgaatt acagtcaagt gccattttac catatttttg gtaccgagct gcgttcttat
    10681 ttctttctta attttctatg atacaagatt ttctctaacg tttctatttt acagaagatg
    10741 ctagacaatt gcaataaacc aaaaaaggct gcgacggttg tcacagcctc tctcgtattt
    10801 tgtgcctatc aatgatagcg ttggatcctg gaagaatgag cgctatctgg ttgttcctca
    10861 taagatgtat catcataagt catatcgtca tatgcctctt ccataacata gaattcctcc
    10921 gcaacttctg gttcatcata tcgatgaacg gccatagcca acaggatata aataatcgga
    10981 acccatgggt atgaagtcaa tgtatcctcc ataaatgact gcatcatcac cgcaaaaaag
    11041 gcccagataa aagtcatctg cccgttggat aaatctgctt tcaacgttaa gcgaccaata
    11101 aaaaggataa gcagcaaagc agcaatccca aatcctatca atccatagat attcaaaata
    11161 gaattataac tattgtggtt tcctaaaaat tggtttcctc gttggaagat aaatgttttc
    11221 attcctagta aaatttgttc agatttttct cctagcgtct gatagaattt atgccaaata
    11281 tcttctcgac cggtaaacaa attgactttt tcagagacag ccgccagata agaaatcggc
    11341 aaagctaaaa gaatcgtaac cgtgtaagca ataaatggcg cacgaatgac acgcatcaac
    11401 gatttaggta aaagatccaa tatcgcaaaa acaatcaacg cgactaatgc acctctagat
    11461 tggcagaccc aaatcccgag aaaagctgca acaactgcta ctgcaccgat gattcgtaca
    11521 taccatcgtt caaaagaact ggcaaatcca ctgatcaata aagcaagagt catcaaagaa
    11581 cttccgatta tattcgtatt gatccaaata tcagaaagcg aattgcctgg agcccagatg
    11641 ctttgtcctt ctggtacttg gatctctgta aatacacgat ataacgtgat tccgaggaag
    11701 accgaaaaga caatcagata aataccgcga tcccattttg tccactcgat ccatttaatt
    11761 actaaacaaa ataaaataat caaaggaaac agtaagtctc caaataaaga ctcatttctt
    11821 aattcccgaa agtaagaaaa gaaaatgaaa agggcaagcg caacacctag ataaagaaaa
    11881 tcgacgcctt ttgctttata aacatttaaa atgagcaaaa tagccacact aaaagcgata
    11941 agtaatgaac tattgttata tataaacaaa gttgctggaa aacctaaccc tgctgtccaa
    12001 acaaaaaaga ctagcgtcag taacaaaagc aagaaaccga cactcatttt gttgacaaat
    12061 ttttgtacga ttgcttgaat tatctccact ctcctttcta ctaacatttc aggattattc
    12121 tcagctagcc tattactgta cgactagctg attttcctga gtttgtgttt gtggtacatc
    12181 tggataatcc ggatattcta ctttaaaaat catataaccg tctttatccg gtccatatag
    12241 ctgcgtgaac tgcgcttcat ataattggtt gtaatcagtt aatggtcgtt tactgatcac
    12301 atagtcgatc cctaattttt ctgcgtcttc aaaattcaac gtgaccgaaa acatatctgg
    12361 acgatctaaa atgaactttg tatcttcctt agcgataaaa gctcttgtat gagcataccg
    12421 attataatac ttctcgtatt cttctttcgg atcgattttc ttccatgcag ctatatctgg
    12481 ataaaagcgt actgtattga aggatttcac tcccaatgtt ggggtgaagt tatataattc
    12541 atcttcagac aaccaaactt taccaggatc ttgttgatcg attttttgga tttcttttgc
    12601 cagtgttttt tcaaaaatcg ctcctgtacc ttctacgact ggattgacga aaaagccaga
    12661 tacgataact aaggcggcaa aagcgaggta aaacaagcgt ctccagccaa ataatgcaaa
    12721 agctaaaatc actgttccca acacagcaac tgcgatggtt tcaaaatccg aaaaataatt
    12781 atccattttc gaatgcgtaa tcgcatagaa ataagaccat gcatttactg ccaacaaaat
    12841 gaatttatac caaataggaa taacatcttc tctccaaata taagcgataa accagatcga
    12901 taataaacaa gccgcaaaac taaaagtaag gatggctctt aatggaggaa cataagacag
    12961 cagagtgatt tctgctagcc attttggcaa cccaacggta atccaaaaga tagaaaacag
    13021 acaaaaaatc atcaaaatac gtcccagtaa cttttgttcg ctatcttttt tgccaaataa
    13081 cacaaatgga cttgctagga atacagttgg aaaaaagtga tagaacaggg ccacttcaga
    13141 gttattcgaa tagttgatat cttcaaaagg aattttccaa tttgtaagga aagaaaaaaa
    13201 ttcgccaatc gtccactttc ctccagtact tacccgtttt cctggatata aggtattcaa
    13261 agaagctaac aatgcgtctt ttgacgttag ccagaaatga accatgactc cgccaataaa
    13321 taaaactgcc cctccaatca acactacatc caacagatcc catgaaactt tcttgctata
    13381 gtaaataagc aggccaacaa aataaaaaat cagcaagtac gccagcatca cttgatgggc
    13441 tgggtaaatc actaaaataa acccaatacc gaaaataacc accaaaagca tcatcaacgt
    13501 tcgtagccat tttttatcat gattgtagaa ataatgataa aaagcagtca tcagtcccaa
    13561 agtaaagaaa atcaaatcgc ccacatgttg catgaaccac cactggactg ctggagaaaa
    13621 tgtcagcgca aatgcaccga ttagcgatag agcttttttt ctcttcgtta atatcatcag
    13681 tacttcgaat ccaagcaaga ccatgacgac taatttcatc gcccaataaa aggaaagtcc
    13741 tcgatctctt ccgaggaaaa agaatcccca gttgaatggt tttccgataa ccgtaatatc
    13801 ctttacagga gaattatacg caatgatcat gttttgtccg tttaatgaat aatcctcgtt
    13861 caccaacgga aagtcctctt cagcttgtga gaggtaaaaa ggtgtctgca ccagccattc
    13921 gtctgaccgc acttcccgat tctgtccaaa aatcacatca gtttttttcc catcgtctct
    13981 ttgcgacaca tatttatccc aaacaccaag tgaacttccg tttaaattaa aagtaacgat
    14041 gaccacgatg actaaaacgc caataagata gcggagtttt agaaacaggt caagcaccaa
    14101 attcgaatct gcctggttcg cttttggctt ttcttttcgt cgtgcacttc ttgtttgttt
    14161 ttccatatca tgctccaaat tctatttgat aaaaggaaat tacttattca tttttttggc
    14221 agcttccagc atttgcgcaa gtgcttcttg ccaagttggt atgacaaatc ctaactcttc
    14281 tgtctttttc aggctcatga ctgaatactg cggacgtgtt gctttttgcg gaaattcttc
    14341 tgacgtaaca ggtgcaactt ctacatctgt atctttcaat atttcttttg cgaattcata
    14401 ccaagaacag ctattttcgt tcgacaaatg ataaatacca aatggtgctt tttcttcgat
    14461 gacatatacc ataaattctg ccaaagtacg tgtccaagtt ggtcgcccaa actggtcatt
    14521 tactacagtc agtttgtcgc gggtttccgc taggcgttgc atcgtgaaga cgaaattgtg
    14581 tccatagatc ccgaaaaccc aagaagttcg tatgatataa tagtcttcta aaatctcttc
    14641 tactgctttt tcgcctaata atttcgtacg accatattca tttagtggat tcgtttggtc
    14701 gtctacttcg tattctacat ttttgttttt tccatcgaaa acatagtctg tactgatata
    14761 gacgaattga gcattcacag cttttgctgc aagtgctaca tttcttgtac catcaacatt
    14821 gatcttctca tctaattctt ttgcttcttc ttctgcttta tctactgctg tgtaagctgc
    14881 acaatggaaa ataacatcag gttttaattc tgtgatgtaa gccattgttt tctcggcatc
    14941 tgtgatatcc aagtcttttg catctgttga aacatattgc agattttttt catctaataa
    15001 atggcgcaat tctgtaccta gttgcccatt tcctccagtt aataaaaaca tatgattatc
    15061 cgctccttga tttttatttt aaaaaagaga caggcgagcg cctgcctcac atgtgttttt
    15121 ccattaatac agtgacattt tctctgtatt attgtccatt tttggcatat gctgcttcaa
    15181 cggcctcttt ttcttttttc caccagtctt catgttcttc gtaccatttg atcgtgtcag
    15241 ccaacccatc acggaagttc gtaaactcag gcttccatcc taactcttcc cgtaattttg
    15301 tcgaatcgat tgcataacgc aagtcgtgtc ctgcacggtc gtttacatgg tcgtacgcat
    15361 cttttggttg acccatcatt tctaagatca attccatcac ggtcttatta tcttcttcac
    15421 cgtctgcacc gatcagatag gtttcgccaa ttctgccttt cgtcaaaatc agccacactg
    15481 ctgaagaatg atcattcgta tggatccagt cacgaacatt ctttccttcg ccatataatt
    15541 ttggacgaat accgcttaag atattcgtta cttggcgagg aataaatttt tcaatatgct
    15601 gataaggacc atagttgttt gaacagtttg aaatggttgc acgtaagcca aaagaacgca
    15661 cccatgcttt tactaacaga tctgaaccag cttttgtaga agaatatgga cttgaaggat
    15721 tatatggtgt ttccgcagtg aatttttctc cttcgccttc cccatgtcct ggcaggtctt
    15781 cacgtaatgg aagatcgccg taaacctcgt cagtagatac gtgatgatag cgtacatcat
    15841 acttgcggca agcttctaaa agtgtatacg taccaatgat attcgtttgt acaaatggga
    15901 aaggatcttt taatgaatta tcgttatggg attctgccgc gtaatgaaca actgcgtccg
    15961 cttcttttac tagacggtcc actaattctg catctgcaac atctcctacg accaattcta
    16021 cgcgatcgct aggaagtcca gccaagttct ctttgtttcc tgcataagtc agtttatcca
    16081 aaacagtgac atgcacttct ggatgattgt ttaccacgta atgaacgaaa ttagagccga
    16141 tgaatccggc acctccggta acaatgatgt ttttcataag aaaatttctc cttaattctt
    16201 agatttctcc gtaaacaaat ggattttctg cttcaaattc ttttaatgtc ggatgacggt
    16261 gatctttttc cgacataatc gctcttgatg gatcaattgg ccaatcaatc gctaaatctg
    16321 gatcatcaaa tgcgatgccc ccatctgctt ctgcattata gtaattatca cacttataaa
    16381 ggaagttcac atttggtgtc aaagtaacga atccatgggc atatccttta ggtacaagca
    16441 actgtcgatg attatgttca gataagataa atccgtccca ttgaccataa gtcgggctac
    16501 ctttgcggat atccactact acatctaaaa ctgctccagt gactacccga atcaattttg
    16561 tctgtgcagc ttctcctttt tggaaatgaa gcccccgaag gacaccagct tcagctgata
    16621 gtgaatggtt gtcttgaaca aagtcatatt tcaatccatg ttctgcaaat tttgcttttg
    16681 aataactttc tgtgaagaaa ccgcgatgat cgccaaagac gtccatttct acgattttga
    16741 catctgttaa ttttgtatct gttactttca tctgtaagga ctccttattc agcggccaaa
    16801 ttcaataaat attgaccgta ttgatttttt ttcaatggct gtgcaagggc cagcaattgt
    16861 tccttatcga tgtagcccat acggtaagca atttcttcca aacaagccac ttttaagttc
    16921 tgacgttttt caattgtttc gataaaggta gatgcttcaa gcaatgattc atgtgttcct
    16981 gtatcaagcc aagcaaatcc gcgtcccata agttctacgg atagttttcc tttatccaaa
    17041 tatgctttat tgacatccgt aatctctagt tctccacgtt ctgaaggttt gataccttta
    17101 gcgatagaaa ttacctcatt gtcataaaaa taaaggccag ttacagcgta atttgatttt
    17161 ggctgagcag gcttttcttc aatcgacaaa gcttgcatat tctcatcaaa ttctactaca
    17221 ccaaagcgtt ctggatcatt gacatgatag ccaaatactg tcgcgcctgt atcttttgaa
    17281 gctgctcgtt gcaacatttt agacagaccg ccaccatagt aaatattgtc ccctaatacc
    17341 aagcagacac tatcatctcc aataaattct tctccaatga taaacgcttg tgccaatcca
    17401 tctggacttt cttgtactgc atattcgatc tgcagtccta aatcatttcc gtttccaaat
    17461 aattccttga aacgtggtgt atcttgtggc gttgaaataa tcagaatttc tttgattccc
    17521 gccaacatca gagtagacat tggataataa atcattggtt tgtcataaat cggcatcaat
    17581 tgtttcgatg ttgcctttgt taaagggtac agacgtgttc cgctgccccc agcaagaatg
    17641 attcccttca tggatatctg ctctcctttt tgatttcaaa tttcactttg ttattatctc
    17701 atcaattgtt atatttgtca tggaaatatt aaggatttaa acatctttta tcaacgttta
    17761 aaccattttc gtttgatcaa cgccgtaatc agatgaacac gccaagaaat tttttgccgt
    17821 aatttagaat gtgtttcaat ctcactcgcg tttaaatcat gtcttcggta cagagttaac
    17881 ggttctctta ggaacccagt cttctttcct cccagcaaac caatccacat atcatgcatc
    17941 ggcacttctg acgggatcgg caaaatatcg ggttttaaag aagaccggaa agccatacca
    18001 gcaccaatat agtgacttct tatcacatta ttaagccatc cttctttgga atttcgaaac
    18061 gaaaaatacg aatcatggat ctcttgcagc tcgctgtcca caatgattaa atcagaaact
    18121 actactagct gatccggatt ctgctcaaaa tagtcagtga tctttttcac ttttgtctcc
    18181 aaccaaacat catcttggtc tgccaaaaag attagatctc ctttcgtctg ctggatcgca
    18241 taaccaaaat tagcgatcaa tccttgacga ggccccgaaa aaagctggat tcgcttatct
    18301 ttttctgcgt attcagttaa tatatcccat gtatcatcta ctgacccatc atcagaaatg
    18361 actacttcat cttctgctga aagctgagat aaaatactat ctaactgctc tcttaaatat
    18421 ctgcctccat taaaggtagc tatacaaacc gaacgcacga attcacacct tccaattctt
    18481 aacatatgag cgcaaagtca tccgccagat atgccggttt ttgacggtca agaactgttt
    18541 agctgttctt aacaataatt gtcgtcggtg actggataaa tgcctttgat catattcatg
    18601 ataaaatcga ttttctgctt gtaaaataga acggtatcga ttacttgaaa ctttttcata
    18661 gtctaaaacc gaaagatcat gttccattcg tacaggaagg atctccacag ttttttccaa
    18721 ctgatccact ttccaaaaca gccaatgatc caaaaaatct aaagaaaaag caggattaaa
    18781 cccgccaatc ttttttagaa atttgacaga ccaggcaaca cctgaattga ttgccatgat
    18841 tcgttcagct gttgggcctt caacttctac tgcttgagct gtacgattga tataacgatc
    18901 tgaatatagg ggagaaactt gtcggctccc agaaaaaatc atcggaactg cagcaacaca
    18961 ttcatctgta aaggttactt tacgcaatag ttcaaaataa tcgtcgacta gcctcgtatc
    19021 ctgatccaga gtgacgagac aacgtgttcc ttcatctgct tgagacaacg caaaattata
    19081 agccaccgct aaaccaggat tttgcggatc atgatgataa acgatatttt ctttttcaaa
    19141 cagctcattt tgctgccttt gaggactatt atcgtaaata ataagctgaa tttgcttttc
    19201 gctaagtgct ttgcttaatg gtccataagc tggagattga ctgaatagtt gctgataaag
    19261 cacaacgact actaatattt ctggtttcat cagaaaaaac gccaccccca cttattgaaa
    19321 aatttcatca tagaagtcaa aaacacgcga aacagttttg catttcgatg cgcacccttc
    19381 ccgtaaaaat ggataactgc ataaaagggt gtgtacaata acttgtaacc tgctttttca
    19441 aaactcaaac acaagtcgtt gtcttcaaag tacatgaaga aacgctcatc aaaccccttt
    19501 atctctttaa atttatcaat atcagctacc ataaaactac ctgagatcat ccgtacataa
    19561 ctattagcat cagtaggcaa tcctcggcat tcaaacacag ccagtcgttt gtcaaacttt
    19621 tttttgaccc atttgaaagg cacaaagcgc aaaacgtaat caaaaacagc cagctgctca
    19681 cggatgaggt gctgagttgt tccatcttca ttaagaatct taggtgctaa catcgcacat
    19741 tccggatgtt ctttcaataa caagaccaat gctgtaactg tctcttcatc cactaaaata
    19801 tctgggttac aaataatgcc atagcgagaa tcgctagcta tcagattgtg attatgtcca
    19861 taaccaaatc cggaattttc atcatgaaaa tagatatcga tgtagggata tttcctatac
    19921 tctgttagtc tttttttgta ctcgggaagt gaattgttgt caaaaattcg gatttttact
    19981 gctgtttcat cttttacagt tgaaataaaa ttatctagtg tccgaaaaat tttatcactg
    20041 tttgccgtca caatactaat aaggatcggc tggtttaatt tgttattcat ctatatgacg
    20101 gttccatttc ttcaaatatt tctgttttac ttgcgtacgg tactctttat tccctaaaaa
    20161 tcgcaatgtt tttaaaataa actgatgttt ctcattgatc agaccaatca attcaaataa
    20221 taaaattgca gcaaacaaca tggcaataat caatatgatc gttccgattc ttccagtata
    20281 attcatgacc atagctgtga aagaaaacat caaagccatc gcataaatcg tcaggacagc
    20341 acctttatga gtaaagccta aactgattag tcggtgatga agatgttttt tatctgctgt
    20401 agaaaaagaa acacggtttg cttttctcct aatgatagca aaaaacgtgt ctgttaccgg
    20461 cactcccatg acgatcagca aagaaatcga tgaaacaaaa gtcacgttct tcagcccttg
    20521 tagtgaaagg acggccatca taaaccctag gaatagagct cctgtatcgc ctaaataaat
    20581 ctttgccgga taaaaattgt aaggaaaaaa accgataata cttgctacaa gacagaaaat
    20641 aacaactgga atatacactg tagaagcatg gaggaaaaaa tatccaatga ttcctatcgt
    20701 aattaaaccg atcaatgaaa tccctgccgc cagcccatcc aaaccgtcaa tcagattgac
    20761 cgcattcgtg ataccgaaga tccaaaaaat cgttacggga aaactaagcc agcctagttg
    20821 gatatatcca ataaaaggaa gcgtgaagga atcaatacgt atatctgcta caaaataaat
    20881 aataagagaa gcaatcaaga tccctatact tttttgttta ggtgtcagct catatttatc
    20941 atcgagcact cctgtgacta tgattactaa agaagcaatc aagatcggta caatatacga
    21001 ttgagggata attttacgga ataagaataa agtcgatata acaaatgaaa taaagatagg
    21061 cagtccgccc gctgaaggca ttggaacttt atttattctt ctttcatttg gtttgtcgac
    21121 catacctgtt tgtactgaaa tgattttgaa tacaggtgtt aatatcagtg ataataaaat
    21181 tgtcagaaaa aatcttataa ccatactaaa agaaacaagc atatgggcac ctactttaat
    21241 taagagtcat aattattata aattatatat gtctttttga ctatcttttt atatcttctc
    21301 aaatgtccgc aaagccttat tttatcggct ctacgggcat tttgcttttg tggtaaacct
    21361 cgcatatcta ggtctatctt cttatatttt ccctgtcaat cgtggttaaa atcgtggtaa
    21421 atacttctat gcgattagac gctgaacctc tgattttgcg gtatctatgg acgcatgagc
    21481 gtaccagttc attgtgatac tgatgtttga atgccccatg atatactgta aatctttcgg
    21541 gttcatgttc ttgcttgcca gccttgtgca gaatgtatgg cgtagcgtat gcggtgtaat
    21601 atgtggcaag ggattgtcct tgtggtgctt gttgtatttc tttaccatac ggataaataa
    21661 ggcgttataa tcaatggcaa cttttggatt acctttcgga ttgataaaca caaaaccgct
    21721 gtgtccgtct acctctatcg actttatctt tggtcggctg ttgataaccc gttgaaatgc
    21781 ctgtatcgtt tctttgctta atggcacttg ccttatcccg cttttcgtct taggcgtttc
    21841 aatataatag ccctgttcct tactctttag taactggtgg tcgataatca caacttcatt
    21901 cttgaaatca atgtcggcta ctgtcagtcc gcacagttcc gagatacgaa gtcctgtctt
    21961 taacagtatc agcacatcat cataatgctt gtgatacacg ttgtccgtct taataaatga
    22021 caataaggct tgctcctgtt cctctgtcaa tgcgactttc tctttggtat cattttctat
    22081 gacttcactt agcttgaaat caaaagggtt tttcctcaca taatcatctt gtatggctat
    22141 gtagaatgac gcttttaacg agcgtttatg gttgttaatg gtattgtagg aaaagccttt
    22201 gtctttcatg cgtaacgccc attctttagc gtcagagggt tttatcgtat caatgctcct
    22261 agcacctaac ttgtcctctt ttaataaccg catgagttgt tcccgttgtt tctgtgtgct
    22321 tttcttcaca tttgtcctct gtgcgttctg tttggcatag agttggcaaa gcgtcatttt
    22381 cttgcctgtg ctgtcgatac cgtcctcaat atcccgtctt atctgctgtt ccagttctcg
    22441 aagtgagggt ttttcccgtt ttccctttgg tgtcgggtct gtgggtgtca atctccaagc
    22501 atacacatat tttgtgtttc ccaatgcgtc tacatattta tataagtatt ttccgtctgt
    22561 tcgttggctc tctccagtat gcaggatacg acctttgtta tcccgtcttt tttctttcat
    22621 ggtgtctgct cctttccttg ttggaaagag ccttgatatg acttgtgttc atcataacac
    22681 atacaaggct catttgcatt agattgcgtc caatttatca ataacctttt caaactgtcg
    22741 gcgtttaatc tgtatgcggt tgccattcat aatgagccag ccagcgtcct tgttttcctc
    22801 tgccaatcgt cttaacttgt tttctccaat gcggaaatac tttgacgctt cttcaatggt
    22861 aagggtgtat ttttcccata cgggaatatc ctgtgtatta ctcataaaac accccctcac
    22921 tttcgattac gtctgatatg tccgtacaca tctgttccac aagcatttcc tgtttttcag
    22981 aaaggtgggt acattcatca atcattttca tgtagtttga acctgtcagc ctgtcgattt
    23041 cctctatcat cacgattgac ggcatgacct gtctttccag ccattgcaga gtgcgttcca
    23101 tattgacagg tacagggttc atttcaaaag gtaaactgtc atgggaaatg aatatctccc
    23161 acaatggata gtcaggaaat tccacaaaat cggtaatgag ataaaacacc agcccatgag
    23221 ggttatacgt caataacagt tcttcaactg cctgtaccgc ctttttgtca cgcagacgaa
    23281 tttcaaaacg gttgataata tcggtatgtt tctttttcgt tgcctgttcc ttttgttttt
    23341 catacaggca gaaatatttt gtgcttgcct ttgagccgat ataaagggta cttgctaaat
    23401 tacggttttt tccgcttaat tttccacctt gtacattttc atagttttta cagcgacatt
    23461 ccgcaccacc atttctgtat ttttcagata gcacaggaat atccagcaga ccgcacatat
    23521 cattgattgc caagtcaaaa cgtcggataa caccgccaca gtcaagacag cggtttaaga
    23581 atgaatacca gtccctgttc tgtgcctgta agacatattc catgttgcgt gagcctgccc
    23641 cttttaattc taaaaatacg cccatgtgtt catcatcaga agccatgacc ttgatttccc
    23701 cgtaagcata atgctcctta tagccataat acccatagtc ataatggata aaataatctg
    23761 ctttcatgcc aagcaccttt cggataactt ccaatgcgtc agtcgttgga aaacggacag
    23821 acagataatc aacgagcaga taaaaaggct tatcgcagat atagtaattc agacaacgca
    23881 ggataatgtt ctgtgtttcc ttgctggcgt tagcttttcc gttctcaaag cggttgatac
    23941 tatatcttga tacatgggta agacttgcaa gttctccttg tgaaagaccg ctggcaagac
    24001 gtttgttctt catttcaagt gcaaattcta aattgttcat tgtattcctc caatcccaca
    24061 atagccttga aatacctgta aaccaccttt caaaaagtgt gatatgtact atcttcttaa
    24121 aacctctcat tactactcac aggtttgggg gtgtatggtt tttgacttgt cttttgtgaa
    24181 atagtgcccc cctgttagat aacgggggca cagacaaggc tcgcaggctc gccagccgac
    24241 aaagcatttc ttccccttga cacaaggtgt catggcggta agcctgagag gcttccgcca
    24301 ccgtgtcaga aatactttgt cagcaagttc tgttttgcct tttctaacaa gttccatttc
    24361 aaccgatatt catatctacg gatttaatca gataggtatt tctttttgaa tgtttttata
    24421 tttcattcta ttgtgcttat tcgattacaa gaacatagta attcttatat gcttatttgt
    24481 caatatcttt ttttgccttt acaaatcata ttaagcatga tagacttata atagcgaaga
    24541 tataacgaga tatggagggt tatgcaatga aaataagcca tttaggaaat aatatacaga
    24601 ccataagaaa atttagggga atgaaacaac aggaacttgc ggacaaaatc ggtatcaata
    24661 tgcagagcct ttccaagatt gaaagagggt tgaactatcc tgcctatgaa acgctggaaa
    24721 agataatgga agtgctggac gttacgccca atgaattgtt gtcgggagaa tggaagtatg
    24781 tcaatcaatc tgaaaaggaa gtctgtcagt ttttaagagc cgaagaacgt ctaaatgcag
    24841 aactgaaaca tggacactat gataacttct ttgacagcga ggaagaatgg ctggaatatg
    24901 agttagaaaa gttacgggaa tatattacag actatatcaa cgggaaaagc attgaagcgt
    24961 ccgaccttta cccaatcaaa gaatttatcc agcatttgaa gtttcaaaaa ttattagacc
    25021 gctatgatga tttatacagt atggatatgt ttggggaaag catagaggga cataagtata
    25081 agacacctta tcaagtcgtg aaaatgataa acccgaactc aaaagaagat atggaactgt
    25141 tacgagaagt attaaggaat aacaattttg atgatgagga cgagtaactt ttttcctctt
    25201 gcgagaagat acagcaaaca aaaagcccga tcaagagtgc aaagcaccac caatggtggc
    25261 aaagctcttg acagggcttt ttcttttctg ttgtgggcaa tcaagaggaa gaaagggata
    25321 aaatctgatt tgtggtaaat caggcaaagg caacgcaggt atcacagtat gaattgacac
    25381 cgcataagga aagcaactgg aagattgtag aataaatgaa aaaggcttgc attctgcatt
    25441 tttataaaat tgcaagtctt tatattgaca agcataaaat actaagatat actttaatca
    25501 gaccaacggt ttgtattgga ggtaagttat ggcacgaaat aaatatccag aagaaacagt
    25561 aaaactcatt ttagatgttt ctacaaaaat gttttgtgaa aaaggatatg ataacacatc
    25621 attgcaagat attatcaatg aaacaaaact ttctaaaggt gctatttatc atcatttcga
    25681 ttcaaaggaa gatattttaa aagcaatttt tcaaagaatt ggaaatgaaa atgcagtaat
    25741 atttgctaaa attagagata acgatagttt aaacggttta gaaaagttaa gagaaatatt
    25801 taagacagcg gtattcaatt ctaaccaaag cgttttattg acagtttctc ctcaactatt
    25861 aaacaatcct cgttttcttg ctatgcaaat agaacaaatg tatgaaattg tagtacctga
    25921 atttgttgaa cccgttttag aacaaggtat caaggataag tctttggaga ttgaaaattc
    25981 acatgaaatt gcagaagtta tcatggtatt aagtaatgta tggctcaacc cattagttga
    26041 aatgacggat aaagagggta tggaaaaacg ttgcaataca ttcaatgccc ttttaaatgg
    26101 aataggcata gacacattat tagacgaaga aacaatttct gcatttatac agttttgtaa
    26161 ataactgtat ttgttatccc tatacgatag actgctctat aaaagagcag tttatttaaa
    26221 ccattatata aaccgacggt ctgtttgatt aaataaaaag gagagatttt atgcaagcag
    26281 aaaaaaaact ttttaatcgt gattttatac ttgttgttat aggacagata atttctttat
    26341 ttggaaacgc tatcctacga tttgcattac cgtactattt gttaaaagaa actggttcat
    26401 cagctttgtt tggtattgct acagcctgct cttttttacc tatggttatt ttgtcgctgg
    26461 taggtggtat tattgctgac aggacaaata aaagaaacat tatggtcata ctagattttg
    26521 taacagcaat cataatttta gtattttatt tgtcattggg aaaaattcct atcatacctt
    26581 tgtttattgt tgctttgatg ttactttatg gtatttctgg tgcatatcaa ccagcggtac
    26641 aagcaagtat tcctctattg gtatcatcag ataaacttac cgtcggtaat gctataataa
    26701 atcaagtaaa tactttagca aactttatcg gaccagctat aagcggagta gtttttcaat
    26761 tttatggtat caatcccatt ttaattgtta gtattatatg ttttactttt tcagccatta
    26821 tggaaatttt tatccatatt ccacaccaaa aaagacctcg tgaagtaggt gtaattgcag
    26881 ttgctaaaaa cgatttaaaa gatagttata cctttttgaa aaaggagaaa cccattttta
    26941 tatccgtaat ctttttggtt tgcattttca atttggtttt aagtgctgtt ttaattgttg
    27001 gtctgcctat cttaatttct gaagttttaa aaatgccaga tacactatat ggtttttcac
    27061 aatcagcatt atcattaggt ggtttatgtg gtggtatttt aacagctctt gttgcttcaa
    27121 agttaaaact aaaaaacctt tataccttgt tacttatttg ttctgtttct gttttactta
    27181 tgggaatttc gctattttta gaattgcctg caatagtttc ctactgggta gttacagtga
    27241 tgtgctttgt ggctatgggt gtatctacta tgtttgttgt tcaaatttac actcttgttc
    27301 agacacaaac accaccgcaa ttagtaggaa agataatggc tacgctggta gcagttgcta
    27361 tgtgcggtca acctattgga caaatggttt acggtatttt attcgatgta tttatacaag
    27421 aaacatgggt tgttatgttt gtagcttcta ttgcttcaat catgatttca atatattcaa
    27481 aaagaatttt tcaaaagtta gagatataat acaaggggtt aaatagtatg ttaggctaaa
    27541 aacaatatat aaaagaaaat ttgaaatctg ctaaaaggat agtgttataa aagaaagagt
    27601 aatattgcag tatttaaggc tcgcacaaat atatgttttg tgtgggcttt tttcttacca
    27661 tttttctgta tttctaaagg gtataaacgg tattccgttt atatatttca gcattagcag
    27721 aaagggggtg agattatgag gacatcttct ttcgagcata tcgtcagatt acagttcaat
    27781 tctttaatgc tgattgtcat taagaacaaa gtgaaaagtc gaaacagaca gcttgcaaga
    27841 cattctaaac gtgaagtatt gttttgtgaa atagccgaaa cgaaacaagt tgaacgtgga
    27901 acgattgata cctgttttta tgattgtgta tcatttaaag tcttgcactt tacaatttat
    27961 gtatctgatg aaacacttgg tacagtttta catagactgt cagaaaaaca gcgtagtgcg
    28021 attttattac gttattttca aggaatgaac gacagagaaa tttcagaact gtaccatgta
    28081 tcacgttctg ctatttgcag tagaagaact agaggactaa ggaagttaaa aatgcttttg
    28141 agcgaaagga agtaaagaaa tgagtaaaga ttatggctat ccctcttttg aattgatttg
    28201 caaagcgtca agcggagatg aaatggcagt taaagagatt ttgaagtttt atgacggtta
    28261 tatttctaaa ttatgcttgc gtccttttta ccactctgaa agtggtaaaa tcattatgca
    28321 ggttgatgaa gaactaaaag gagaaattca tacagacatg atgaaagcaa tattgaaatt
    28381 tgaaatcgga gtgaagtaat caactgtata aaaaaatcgg gagatacaca gtttcgtgct
    28441 atctccctta gtagttttac ggtcatatca aggctcattc aatcgtggta aattcgtggt
    28501 aagatgagtg tttttctata cttcaaaatg cttgaatgta tagtgtttat gcggataatt
    28561 gaaaattaga tataaaattt caa
//
LOCUS       DASJST010000022        28362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJST010000022 DASJST010000000
VERSION     DASJST010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.022"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(218..1015)
                     /locus_tag="U4B05_000738"
     CDS             complement(218..1015)
                     /locus_tag="U4B05_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN2049893.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="U4B05_000739"
     CDS             complement(1012..2196)
                     /locus_tag="U4B05_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2049894.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="U4B05_000740"
     CDS             complement(2221..3468)
                     /locus_tag="U4B05_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2049895.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="U4B05_000741"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="U4B05_000741"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN2049896.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="U4B05_000742"
     CDS             5448..5762
                     /locus_tag="U4B05_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN2049897.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="U4B05_000743"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="U4B05_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN2049898.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="U4B05_000744"
     CDS             6447..7091
                     /locus_tag="U4B05_000744"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN2049899.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="U4B05_000745"
     CDS             7106..7435
                     /locus_tag="U4B05_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN2049900.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="U4B05_000746"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="U4B05_000746"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN2049901.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="U4B05_000747"
     CDS             8423..9247
                     /locus_tag="U4B05_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN2049902.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="U4B05_000748"
     CDS             9240..9587
                     /locus_tag="U4B05_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN2049903.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="U4B05_000749"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="U4B05_000749"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN2049904.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="U4B05_000750"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="U4B05_000750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN2049905.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="U4B05_000751"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="U4B05_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN2049906.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="U4B05_000752"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="U4B05_000752"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN2049907.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15217..16011
                     /locus_tag="U4B05_000753"
     CDS             15217..16011
                     /locus_tag="U4B05_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2049908.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="U4B05_000754"
     CDS             16001..18043
                     /locus_tag="U4B05_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049909.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="U4B05_000755"
     CDS             18324..18473
                     /locus_tag="U4B05_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN2049910.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="U4B05_000756"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="U4B05_000756"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN2049911.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="U4B05_000757"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="U4B05_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN2049912.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="U4B05_000758"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="U4B05_000758"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN2049913.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="U4B05_000759"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="U4B05_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN2049914.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="U4B05_000760"
     CDS             22890..23972
                     /locus_tag="U4B05_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN2049915.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="U4B05_000761"
     CDS             23972..24775
                     /locus_tag="U4B05_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2049916.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="U4B05_000762"
     CDS             24845..25585
                     /locus_tag="U4B05_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2049917.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="U4B05_000763"
     CDS             25596..26600
                     /locus_tag="U4B05_000763"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN2049918.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28342)
                     /locus_tag="U4B05_000764"
     CDS             complement(26645..28342)
                     /locus_tag="U4B05_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049919.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8920 a         5026 c         5851 g         8565 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg ag
//
LOCUS       DASJST010000023        27679 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJST010000023 DASJST010000000
VERSION     DASJST010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.023"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(64..1341)
                     /locus_tag="U4B05_000765"
     CDS             complement(64..1341)
                     /locus_tag="U4B05_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049920.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1420..2064)
                     /gene="pcp"
                     /locus_tag="U4B05_000766"
     CDS             complement(1420..2064)
                     /gene="pcp"
                     /locus_tag="U4B05_000766"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN2049921.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2263..3207)
                     /locus_tag="U4B05_000767"
     CDS             complement(2263..3207)
                     /locus_tag="U4B05_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN2049922.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3209..3895)
                     /locus_tag="U4B05_000768"
     CDS             complement(3209..3895)
                     /locus_tag="U4B05_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN2049923.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4369..4689
                     /locus_tag="U4B05_000769"
     CDS             4369..4689
                     /locus_tag="U4B05_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN2049924.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4843..5748
                     /locus_tag="U4B05_000770"
     CDS             4843..5748
                     /locus_tag="U4B05_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2049925.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5745..6518
                     /locus_tag="U4B05_000771"
     CDS             5745..6518
                     /locus_tag="U4B05_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2049926.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6699..8123)
                     /locus_tag="U4B05_000772"
     CDS             complement(6699..8123)
                     /locus_tag="U4B05_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2049927.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8334..8780
                     /gene="nrdI"
                     /locus_tag="U4B05_000773"
     CDS             8334..8780
                     /gene="nrdI"
                     /locus_tag="U4B05_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN2049928.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(8845..10827)
                     /locus_tag="U4B05_000774"
     CDS             complement(8845..10827)
                     /locus_tag="U4B05_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2049929.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10841..11737)
                     /gene="murQ"
                     /locus_tag="U4B05_000775"
     CDS             complement(10841..11737)
                     /gene="murQ"
                     /locus_tag="U4B05_000775"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN2049930.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(11740..12801)
                     /locus_tag="U4B05_000776"
     CDS             complement(11740..12801)
                     /locus_tag="U4B05_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2049931.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(13012..13578)
                     /locus_tag="U4B05_000777"
     CDS             complement(13012..13578)
                     /locus_tag="U4B05_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2049932.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(13606..15108)
                     /gene="eat(A)"
                     /locus_tag="U4B05_000778"
     CDS             complement(13606..15108)
                     /gene="eat(A)"
                     /locus_tag="U4B05_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2049933.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15366..16487)
                     /locus_tag="U4B05_000779"
     CDS             complement(15366..16487)
                     /locus_tag="U4B05_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2049934.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16613..17554
                     /locus_tag="U4B05_000780"
     CDS             16613..17554
                     /locus_tag="U4B05_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2049935.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17704..18600)
                     /locus_tag="U4B05_000781"
     CDS             complement(17704..18600)
                     /locus_tag="U4B05_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2049936.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            18869..20209
                     /locus_tag="U4B05_000782"
     CDS             18869..20209
                     /locus_tag="U4B05_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2049937.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20427..20870
                     /locus_tag="U4B05_000783"
     CDS             20427..20870
                     /locus_tag="U4B05_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN2049938.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21374..22792)
                     /gene="nhaC"
                     /locus_tag="U4B05_000784"
     CDS             complement(21374..22792)
                     /gene="nhaC"
                     /locus_tag="U4B05_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2049939.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23195..24418)
                     /locus_tag="U4B05_000785"
     CDS             complement(23195..24418)
                     /locus_tag="U4B05_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN2049940.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24449..25288)
                     /locus_tag="U4B05_000786"
     CDS             complement(24449..25288)
                     /locus_tag="U4B05_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2049941.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25305..25985)
                     /locus_tag="U4B05_000787"
     CDS             complement(25305..25985)
                     /locus_tag="U4B05_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2049942.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(25987..27060)
                     /locus_tag="U4B05_000788"
     CDS             complement(25987..27060)
                     /locus_tag="U4B05_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2049943.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(27116..27354)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(27397..27630)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         8006 a         5806 c         4934 g         8933 t
ORIGIN      
        1 aaatagaaaa tagaggagaa catcattcgt caatgatgtt cccctctaat cttagtatgt
       61 tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc
      121 taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc
      181 tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg
      241 ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc
      301 tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc
      361 ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat
      421 gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg
      481 ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac
      541 ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa
      601 ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg
      661 tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc
      721 ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc
      781 taattttata tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca
      841 gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc
      901 gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga
      961 gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg
     1021 cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg
     1081 gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc
     1141 agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac
     1201 tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta
     1261 ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa
     1321 gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac
     1381 taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt
     1441 tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc
     1501 aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga
     1561 gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag
     1621 caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg
     1681 tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc
     1741 ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat
     1801 tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc
     1861 ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg
     1921 actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc
     1981 atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc
     2041 aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt
     2101 tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata
     2161 acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag
     2221 aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat
     2281 ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc
     2341 gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg
     2401 tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt
     2461 cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc
     2521 aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc
     2581 gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac
     2641 tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg
     2701 ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg
     2761 agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc
     2821 acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat
     2881 aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg
     2941 gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc
     3001 accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca
     3061 gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt
     3121 gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa
     3181 aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg
     3241 ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca
     3301 ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg
     3361 ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc
     3421 agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt
     3481 ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc
     3541 cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa
     3601 tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt
     3661 ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca
     3721 tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac
     3781 cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa
     3841 atgcaaaccc gagaataaca atcaagacgc ctaaaaggct aagataattt tccatatatt
     3901 ttcctccata aaatgacaat atttttttaa ttataaagca aaacacataa aaaatatagt
     3961 tgttttctca cgaaatagac actattttta aatatttttc gttatttttt tatcacaaaa
     4021 attatcttac ttattgtaag gtaaagcttt ttttaattta ttgcccgata atatatagaa
     4081 ataaggtcta gttactgttt tttttaaatg attaataaat aacaacttct ttctatgaaa
     4141 ttgcaaagca ttcatcttct tttccgctaa cgatatatcc gtcagtgctt ggtacacttt
     4201 actaaaaagt atgagattgc cgatatctgg ataggtgtgc tatactacag aaaaagatat
     4261 acaatctgcc accgggtagg ataaagagga tgatcattcc attaggtctt tgaagggatg
     4321 attttcctct ttttgtgtat ttttttatag ttaaggagga atgtaagtat ggcatggatg
     4381 gaattaatca tagcaggaat tttggaagta ttctggtcaa cgatgatgaa atggagtgat
     4441 ggtttttcta aaatcaatta ttctttttac acagtgattg gcatgattgc tagtttttac
     4501 tttttgtcaa aggcaatcaa aagtttgccg atgagtctct cttatcctat ctggacaggg
     4561 attggcgcag ttggttcggt cataattggt gtggtcttgt ttcatgataa gctgaatctt
     4621 tccacttggt tctttgtggg gatgctactc atcagtatca ttggcatcaa gattactagc
     4681 ggccactaga cacttggaat aagtactgat cagaaaaaat aagccttttt atttaatatt
     4741 caattttttt atgaaaaaat aagaaataag tccaatgacc tagcgacttc tccactcatc
     4801 aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc
     4861 caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta
     4921 tcccctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg
     4981 aaagctattt taggactgat ccattactca gatggaacaa tctgtattgg tgaaaatgag
     5041 atttctccct ctagtcataa agggttggaa caagtaggag cattgatcga atacccgggt
     5101 atctatccat tcttaacagg ctatgatcat ctgaagttat tctctgaaac caacgatgta
     5161 tctgctatcg acactatcgt gaatcagctc aagatggaaa agtacattca caaaaaagcg
     5221 aaatcctact cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat
     5281 ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat
     5341 gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac
     5401 ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaagatc
     5461 atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct
     5521 acaacaaatg atctagctgc tcgaacgatc ttgacagaag ctggatatac tttggaagat
     5581 cctgataaaa tcaagctttt aaaagaaaac gaagaattat taggaaccgt gatccagcta
     5641 ttgtctgcac aaaatatcca tgtgacagac gtccagcatc tgacgaatga tttggaatct
     5701 tctctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgaca ttgcttgtaa
     5761 aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag
     5821 ttgttttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag
     5881 ttgaaatgac tgctcagctt tttagtgcca catcttggat tgtctttatc atgattgcag
     5941 ctgcaagcac gatcatttcg atggaagcac aatatggtac attgaaaaat ctcctttatc
     6001 gaaagtattc tcgaggagaa atattagtaa gcaaatggat cacattagtt atttattctg
     6061 tttatttata ccttttagct attattgtca ctgtgttgat gaaactgatt cttttccctt
     6121 cgatttcctt cacggagaag gtttctactg gtcaaacact gatccaaagc ttgttcactt
     6181 atactttggg tagttatatc ggtttatggc tgattttaag tttagtgttg atgattgctt
     6241 gctttatcaa tagttccggc gcatccatct cagcaggaat cgtcttttat tttgcttcgt
     6301 caatcatatc tggtatcctt attgcactca tccaaaaatg ggagtggatc aaatggaatc
     6361 cgatcagtat gttgaatctg caaaaccaaa ttggaaacga agagatcatg aagcaattga
     6421 ctcatctttc tactaatcaa atgctattcg gtaatttagc ttacatcgtt ttgttcttag
     6481 cattaggata tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa aaagaggctg
     6541 tgaaagagac gttcagcctt attgccgatt ggctgtctct taaacaccgt ttattctgaa
     6601 aaaggagctg tgacaacatt tgtgtcacag ctcctttcct agtatgtttt tgaataaacg
     6661 acaggctcac aacctctctt tatacttctt ttatacttct atacgtgaag catcccagtg
     6721 gcaaataaaa ccattgcaat cactccgacc atcgtcacac ctataagtaa aatcttatct
     6781 aagtggatat gttgtacagg ctcgttttgc tccttacgtg ctttgaggta aaaataaatg
     6841 cctggcaaga aaagaacaag aacaagcatt acttcattaa aggctacgac tagcatagcc
     6901 catacttgaa aggcacttgc taaaagccct actaaaaatt ggacccattc tttccgctga
     6961 taagaaagtt tcatttggta caatcctaag aaaagccacg tgaagaaaat agctgctgta
     7021 ccaagtgtgt aagcaaagtt ataagcactg tcagtgaata ataatgaaag taaaaagaca
     7081 ttcgtacata acgtcgtcaa tacgatagca taagttggtg ccccattttt gttgagtttt
     7141 ccccatgctt taggcaaaag tccgtcttca gccatgtttc ccattgtttc aatcggtaag
     7201 atcgtccatg agagccaaac accaactaaa gaaatgatca aaccgccgtt gatcacgact
     7261 gctccccatt taccaaccat catttcaaac acatagccca tagcaggctg cgccttgatc
     7321 gtttgcagct gattcatcgt cattgtccca tatggaagca tggaaaccaa gacgtaaata
     7381 ataatcaagc cgaatatccc tagaatcgtt gctttcccaa catctgattg tttttttgct
     7441 cgtgaaccta cgacagaggc tccttcaata ccaacaaata cccaaagcat caccatgatg
     7501 caactcaata cttgactgga aaacgggatc ggctgtccat tcgcattcaa tataatgtcg
     7561 ctagaaaagt tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac tacaataaat
     7621 aaaaataatg gcactaattt acagaatgtg acgatagcat tgacgaaact agcattttcg
     7681 actccccgat tcactagcca tgcatatccc cataaaaaga ctgtactcat cacgatcgat
     7741 gccagatttt gcccaccttc aaatactggg aagaaatatc ctaatgcgct catcaacaat
     7801 gttgcaaaag ctacgttgcc taaccagcat gtgagccagt aaccccaacc gctgataaaa
     7861 ccagcgaaac ggccaaaacc ttcttctgcg taactaaaga tcccgccttt taaatctgga
     7921 cgttttttat tgatattatt gatcgacaag gagagcatca acattgctgt tcctacgagt
     7981 acccaactga aaatagctgg gccgccagcc gatccggaga tgtccgtcat aataccaaat
     8041 acgccacctc caatcgctga accaattact aaggcagcaa gtggaattaa accaattcct
     8101 tttttctgtt gctctgtatc catgtaagca tcctccaaaa taaaaccgct tttatcaaaa
     8161 agtataacaa acctttttta tttttactta attaataaaa actttaatag aaatataatt
     8221 ctaccggctt ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac
     8281 caaaaaactt aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca
     8341 ttttatatat atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg
     8401 ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg
     8461 aaaatagtga ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag
     8521 acggcggaaa tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat
     8581 atctggaaca tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa
     8641 acttcaataa tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt
     8701 tcttggcaga ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt
     8761 tagcagaaaa caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct
     8821 ttcgtttcta ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt
     8881 tgctttacct gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac
     8941 gatcatggat gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag
     9001 cgtatcaggt gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa
     9061 gtcaactgtg ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc
     9121 aatagcgtgt ttcgttggga agacagagct gactacccca gtaactggag aatagatatg
     9181 tccgttttct ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc
     9241 acttgcttta tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact
     9301 ttcagctgaa gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt
     9361 cgctaattct tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa
     9421 gccaccagca taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat
     9481 caatgggatc aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat
     9541 tccgatcact gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag
     9601 aggtaatgtt actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc
     9661 tcctttgatc atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc
     9721 tgcacccact tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt
     9781 ttcatcaatc atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat
     9841 cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca
     9901 gttgatccca ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat
     9961 ttcaatcaga ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc
    10021 aggtactact ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac
    10081 acctaggatc ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg
    10141 tgcttccgga ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg
    10201 tgttgcacca aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat
    10261 cccgtttttc aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt
    10321 tccggctgtg actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac
    10381 aaatgcagga atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt
    10441 agatggtttt tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg
    10501 gtcttttcca gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc
    10561 taatttcacg cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc
    10621 gatgatgact tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa
    10681 ttcagagaca tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg
    10741 gacaatgctt tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc
    10801 ttccaaagtc agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa
    10861 atccgtttgc ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca
    10921 tgacgatagc cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt
    10981 aatcagcttc tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg
    11041 ttggtttgac atcgaccatc aagttattga atactttacc tataccaatc atactgatag
    11101 tagaaatcat attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg
    11161 tcaaaaattc tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg
    11221 cttgtttatt gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc
    11281 caccgatcac atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg
    11341 tcaaattcag cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga
    11401 cagctacagt cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta
    11461 caccgaatgt cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg
    11521 ctcccatata aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg
    11581 gttcgatact tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca
    11641 ttagttgaac ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc
    11701 gttccgttgt taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat
    11761 aaattgatag ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac
    11821 tttagctact acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc
    11881 acccatatag cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc
    11941 tgtatttctt gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac
    12001 atctcttgca tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc
    12061 atctacaggt tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc
    12121 ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa
    12181 tagatctaga gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc
    12241 ttgatatagt gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc
    12301 ttgaattttg agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg
    12361 acgtggataa taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa
    12421 ttcatcgata tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga
    12481 ccagtcggca atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat
    12541 tgctttcaaa ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga
    12601 aatatcgacc atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg
    12661 cttttgatat gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa
    12721 gccattggat gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat
    12781 aaatattgaa aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga
    12841 ctagagtata tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca
    12901 tttctaaagg ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct
    12961 aagcaaaagc agtgccttga aatattttat ttaaataaca caaaaacata attactcata
    13021 agaaaccaat tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg
    13081 aaaataagca ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc
    13141 ttcgtcttcg atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga
    13201 ctccccattt gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt
    13261 gcttggcagt ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag
    13321 cggcaagctg agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga
    13381 atggatgatt ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc
    13441 ctgcatctgt cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc
    13501 gttccaagtt gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc
    13561 aaagttcccc ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag
    13621 agaaataatc tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat
    13681 ggcaggtttc acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata
    13741 attcaatggt tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc
    13801 cacttttttc cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc
    13861 catcccaagc tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc
    13921 aaattccgcc aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag
    13981 atgttttggc tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg
    14041 gataatggat gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc
    14101 gattgtaaaa tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac
    14161 gcttaaaagt cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc
    14221 gatatctttt aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc
    14281 tttggatcgt ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct
    14341 agattcagta tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga
    14401 ccattcggct ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc
    14461 attttgagcc atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt
    14521 cccttgataa agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc
    14581 gataaatccc cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc
    14641 cgctacttgt tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa
    14701 atgagtgtca tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag
    14761 tgtgctgaat tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc
    14821 gatttcccat atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt
    14881 atcttttgtc tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata
    14941 aggtagtttg ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat
    15001 aagtcctagt ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc
    15061 ttggctgtcg tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct
    15121 cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac
    15181 acacaaaaag acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt
    15241 ccttgtctcc acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat
    15301 gcacggatcc ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt
    15361 gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt
    15421 ttttctaatg tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata
    15481 cttaagacaa ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa
    15541 atagcgatta ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt
    15601 ccgtggatcg tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg
    15661 atcttttttt ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct
    15721 cccaacaaaa aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct
    15781 aaatccacct cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag
    15841 aaagctagcg tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt
    15901 tgacctgtat atggattggt aacatctgca gcgataatca attttacttt agaaacaaga
    15961 acgtttcttc gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac
    16021 ccttctaaag aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt
    16081 cctcctaaag tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct
    16141 cctaaggcat aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct
    16201 aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat
    16261 aaatagctga tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt
    16321 cccctgagcg cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca
    16381 ttgatttttc ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat
    16441 gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac
    16501 tttcttctta agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt
    16561 ccatttttct atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat
    16621 cgcaatagct ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg
    16681 aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg
    16741 gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga
    16801 agaaatcatc gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca
    16861 attagatgat atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg
    16921 tttactaaat ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat
    16981 cttatttggg atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt
    17041 aggtgatgga gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa
    17101 agttgtagaa gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta
    17161 ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga
    17221 ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt
    17281 atctgaattt gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta
    17341 ccaacatatt gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta
    17401 ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg
    17461 gcgaaaagga caaaaatatg atattgctac accatattgt gcctttttga cccaattagt
    17521 ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga
    17581 atcagcagct ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct
    17641 catgatgcca taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt
    17701 tttttatttg ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc
    17761 gttagtgata aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag
    17821 gattggctta aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag
    17881 caaaaaaata ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac
    17941 tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc
    18001 ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt
    18061 aagatatcca ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc
    18121 tctttttata aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc
    18181 accaaaaaat agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa
    18241 tgaactatta tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc
    18301 atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg
    18361 attccaaaaa aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc
    18421 catatcaaat ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc
    18481 tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat
    18541 ggaaggcaaa aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat
    18601 cttttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa
    18661 gccagtaaaa cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga
    18721 aataaatcat tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg
    18781 aaaatttgtt atcattagtc attgttaaat attgctattt attcttaata taaattttga
    18841 ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag
    18901 ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg
    18961 aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg
    19021 gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac
    19081 gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac
    19141 tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc
    19201 ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc
    19261 tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg
    19321 gctggctttt gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt
    19381 cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg
    19441 tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc
    19501 ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc
    19561 gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt
    19621 gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt
    19681 tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat
    19741 tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt
    19801 cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca
    19861 agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc
    19921 ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt
    19981 attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt
    20041 atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat
    20101 cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta
    20161 tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa
    20221 aattttattt tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag
    20281 tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc
    20341 aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag
    20401 gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg
    20461 gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata
    20521 tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca
    20581 tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag
    20641 ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca
    20701 acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta
    20761 aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac
    20821 aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg
    20881 tccaaataaa aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta
    20941 cttctggaac gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa
    21001 cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc
    21061 aagcttttga acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag
    21121 agagacaaat ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    21181 cagctgattg aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta
    21241 agaaaaattt tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa
    21301 actggctttt gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc
    21361 cctttttatg tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca
    21421 caaatttttt tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa
    21481 gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta
    21541 cgaaacttcc ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta
    21601 gcgttctacc taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat
    21661 taccaggcag aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg
    21721 tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca
    21781 tcacggttga gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa
    21841 tcaatgacac agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt
    21901 gttggtttcc agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg
    21961 aagtttgtgg gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg
    22021 ttaaaattgc cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg
    22081 accagattga aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa
    22141 gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg
    22201 ccgggacagt tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag
    22261 ctgctgctag atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg
    22321 aaacaatagc tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa
    22381 atgcaatccc aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg
    22441 cacacacaac aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga
    22501 ccattagagt tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa
    22561 taaaaataaa gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc
    22621 aatctgtcgg gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga
    22681 tcgtgatatt aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa
    22741 ccattaatac aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct
    22801 ctcctttttt agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca
    22861 attcatccgc gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat
    22921 gactccgcgg taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc
    22981 aaatgtatgg actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc
    23041 gactttctga acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta
    23101 ccttccataa gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta
    23161 tttttataac tttcacacac aagattattt tttactagaa agtagcgccc tcttgttttt
    23221 ttcttgctac tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca
    23281 acagatcaag agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt
    23341 tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga
    23401 ttgctaccgt aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac
    23461 cgagacttgt tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat
    23521 gctggaagaa taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga
    23581 acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca
    23641 ctttttcata tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag
    23701 cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat
    23761 tgacatcttt tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg
    23821 cttccaaagc atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa
    23881 atttgatgtc gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg
    23941 gatggacgca taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt
    24001 tagctgatcc gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac
    24061 cagtttcatc aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca
    24121 aattatgccc gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa
    24181 ttgtgggacc gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta
    24241 cttctttttc tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat
    24301 aaaactcata gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt
    24361 ctggtaaacg atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa
    24421 aaaattcctc ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca
    24481 cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt
    24541 ctactatttt ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca
    24601 cataaggacg tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa
    24661 atccagcatc tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac
    24721 gtgctgtttg ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat
    24781 cagaaacagt gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca
    24841 ataacagaag agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt
    24901 ctttgatatc agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag
    24961 tatttccgat cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt
    25021 gatgttggaa agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt
    25081 aatctgtaaa tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt
    25141 tgtcccatac atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt
    25201 cagttgtatc tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa
    25261 tgactacaaa acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt
    25321 ttaatcagtt gattactgat aaattgactt aaaaaaacta agattagaat caataatgta
    25381 gccacaatcg ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct
    25441 aatccgcctg cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc
    25501 aacgccgaga cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata
    25561 ccgattggac tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc
    25621 agtgcgttct cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa
    25681 gctgctttcg caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa
    25741 agcaagataa tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat
    25801 agcagacggt tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg
    25861 attccaatca tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc
    25921 gcttcgacaa attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca
    25981 gtcattttat cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa
    26041 aatatttatt tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt
    26101 ttctccagct aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt
    26161 gccatataaa atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc
    26221 gttcgtctga tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc
    26281 aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat
    26341 aaaatctttg gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc
    26401 aacgattctt ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat
    26461 ctcatgagtg atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc
    26521 aaggatctga agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac
    26581 cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag
    26641 ttgtttagga taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac
    26701 tttttgacga cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc
    26761 aaaaatagtt cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct
    26821 ttcttttcgc aattctttgg gagagagctg actcagattc gtctgattga caatgacttc
    26881 tcctgctgtt ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc
    26941 tgagtaaccg acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac
    27001 tgcctgtatc tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat
    27061 taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga
    27121 ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt
    27181 caagtacgat catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc
    27241 tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac
    27301 cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca
    27361 aaaaaaaagt ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac
    27421 cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt
    27481 tcgttttaac ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg
    27541 ccattttcag cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc
    27601 caaacttact ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag
    27661 gaaaactgaa aagtcaagt
//
LOCUS       DASJST010000024        27473 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJST010000024 DASJST010000000
VERSION     DASJST010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.024"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..323)
                     /locus_tag="U4B05_000789"
     CDS             complement(<1..323)
                     /locus_tag="U4B05_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049944.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNV"
     gene            complement(395..1675)
                     /locus_tag="U4B05_000790"
     CDS             complement(395..1675)
                     /locus_tag="U4B05_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN2049945.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(1766..2218)
                     /locus_tag="U4B05_000791"
     CDS             complement(1766..2218)
                     /locus_tag="U4B05_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2049946.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(2231..2641)
                     /locus_tag="U4B05_000792"
     CDS             complement(2231..2641)
                     /locus_tag="U4B05_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2049947.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2916..3128
                     /locus_tag="U4B05_000793"
     CDS             2916..3128
                     /locus_tag="U4B05_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2049948.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            3131..3343
                     /locus_tag="U4B05_000794"
     CDS             3131..3343
                     /locus_tag="U4B05_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049949.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            3483..3638
                     /locus_tag="U4B05_000795"
     CDS             3483..3638
                     /locus_tag="U4B05_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049950.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3635..3859)
                     /locus_tag="U4B05_000796"
     CDS             complement(3635..3859)
                     /locus_tag="U4B05_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2049951.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3937..4125
                     /locus_tag="U4B05_000797"
     CDS             3937..4125
                     /locus_tag="U4B05_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049952.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            4451..4786
                     /locus_tag="U4B05_000798"
     CDS             4451..4786
                     /locus_tag="U4B05_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049953.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5019..5960
                     /locus_tag="U4B05_000799"
     CDS             5019..5960
                     /locus_tag="U4B05_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN2049954.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5962..6852
                     /locus_tag="U4B05_000800"
     CDS             5962..6852
                     /locus_tag="U4B05_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN2049955.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6895..7695
                     /locus_tag="U4B05_000801"
     CDS             6895..7695
                     /locus_tag="U4B05_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2049956.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7710..8561
                     /locus_tag="U4B05_000802"
     CDS             7710..8561
                     /locus_tag="U4B05_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2049957.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8762..9223
                     /locus_tag="U4B05_000803"
     CDS             8762..9223
                     /locus_tag="U4B05_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2049958.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            9249..9701
                     /locus_tag="U4B05_000804"
     CDS             9249..9701
                     /locus_tag="U4B05_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049959.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9807..10001
                     /locus_tag="U4B05_000805"
     CDS             9807..10001
                     /locus_tag="U4B05_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049960.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            10032..10325
                     /locus_tag="U4B05_000806"
     CDS             10032..10325
                     /locus_tag="U4B05_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049961.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            10355..10795
                     /locus_tag="U4B05_000807"
     CDS             10355..10795
                     /locus_tag="U4B05_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2049962.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            10870..11337
                     /locus_tag="U4B05_000808"
     CDS             10870..11337
                     /locus_tag="U4B05_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN2049963.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            12084..12314
                     /locus_tag="U4B05_000809"
     CDS             12084..12314
                     /locus_tag="U4B05_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049964.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            12473..13306
                     /locus_tag="U4B05_000810"
     CDS             12473..13306
                     /locus_tag="U4B05_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN2049965.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13299..14714
                     /locus_tag="U4B05_000811"
     CDS             13299..14714
                     /locus_tag="U4B05_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2049966.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14726..16255
                     /locus_tag="U4B05_000812"
     CDS             14726..16255
                     /locus_tag="U4B05_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2049967.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16173..17225
                     /locus_tag="U4B05_000813"
     CDS             16173..17225
                     /locus_tag="U4B05_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN2049968.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17227..17544
                     /locus_tag="U4B05_000814"
     CDS             17227..17544
                     /locus_tag="U4B05_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049969.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17671..18294
                     /locus_tag="U4B05_000815"
     CDS             17671..18294
                     /locus_tag="U4B05_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN2049970.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            18309..19199
                     /locus_tag="U4B05_000816"
     CDS             18309..19199
                     /locus_tag="U4B05_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN2049971.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19222..19437
                     /locus_tag="U4B05_000817"
     CDS             19222..19437
                     /locus_tag="U4B05_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049972.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19449..19790
                     /locus_tag="U4B05_000818"
     CDS             19449..19790
                     /locus_tag="U4B05_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049973.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19790..20161
                     /locus_tag="U4B05_000819"
     CDS             19790..20161
                     /locus_tag="U4B05_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049974.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20154..20549
                     /locus_tag="U4B05_000820"
     CDS             20154..20549
                     /locus_tag="U4B05_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2049975.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20551..20928
                     /locus_tag="U4B05_000821"
     CDS             20551..20928
                     /locus_tag="U4B05_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049976.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20929..21537
                     /locus_tag="U4B05_000822"
     CDS             20929..21537
                     /locus_tag="U4B05_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2049977.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21537..21878
                     /locus_tag="U4B05_000823"
     CDS             21537..21878
                     /locus_tag="U4B05_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049978.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22120..24909
                     /locus_tag="U4B05_000824"
     CDS             22120..24909
                     /locus_tag="U4B05_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN2049979.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            24899..25639
                     /locus_tag="U4B05_000825"
     CDS             24899..25639
                     /locus_tag="U4B05_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2049980.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            25636..>27473
                     /locus_tag="U4B05_000826"
     CDS             25636..>27473
                     /locus_tag="U4B05_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2049981.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9804 a         3955 c         5443 g         8271 t
ORIGIN      
        1 acgtttttta caagtacatc atcttttata tattttgaaa ttaatgatag ataaacctga
       61 gttgttcgat aggaactttc ttttacagtt ggcttgtatt tttcatccca tttagttttt
      121 aattctccaa aagttagtgg taattcttca tttcgtttta ttttttctgc gattttttca
      181 ttcaaaagta agttagcttg attccaagct tggcgtgaat tgctagataa agtaacggac
      241 acttttctct ttttttcagt taagggatcc gtataacgtt cggtgtactt aaattttttg
      301 tcatttattt tttctaacca cattatttct attcctttcg aatatacgtt cctattaatt
      361 gttaaaagaa aaagcccgaa ggctttttat atatttaatc ttttgacaat tctaaagatc
      421 gtctatattg ttcagcttta gctaattttg taaatgttac tgttccatta taattttcta
      481 aaacaacttt ctctatttcg tctagtgaaa ctttgaaaaa ttcttttctt tgattaattt
      541 tattaacttc ttgttttcta aaatgattat gcaatttagt ttccaaagac ggagcgtcct
      601 ctgaaaaaat catcgcatga acgtcaaatt caaatggaac cgatgcacta ctcaattctt
      661 ttattctatc cataggttct aatcttctag tcataccaat tttatatatg tcttcaccaa
      721 aagacccaat attactaatt acatacacat aaccggctct agtatttaat tctctttgta
      781 atacattttc acgtataact tctaattctt ttaatttttc ttccagttct tgaattttat
      841 ttgcatataa ttctttttcc acatctgcat tagctttttg catatatgcg attaatttgg
      901 aaatttcaga attgaattga cgttgttctt tatcaatacg tttcttttca cgatcaattt
      961 ctctacgaac tttttcctct tctatcatct gttcttttat tgctttttgt tgttcttttt
     1021 cttcggtaat tttcactaaa taagagtaat gaagttctag ttcttgaagt ttaaactcta
     1081 aaagaccctt ccttaattca gcattgtcat tcttgaataa attattaatt ttatcaaata
     1141 tttttatgat tttttcccta gtcgagtcta tattagaaat agttagttta ttgagtaatc
     1201 ctttcacttc tgaatcaaat ggaatgagca tttttttctt taaattattt ttctctcgtt
     1261 tatttaaaat aagagggaca gaaacagcat cgtcaataat atattccttt tcttttaatt
     1321 ttaacaaaga aagcttattt tttatttcag aagatgaagt ttcttctata tttgaaacga
     1381 taagtgaaga atagacattt tttaatgttt catcatactt ttgatttatt tcatcattta
     1441 aatcttcttt ctcttttttt aataaaataa tatcattttt taacatagta atttgtttat
     1501 ttaaatctga tagtgtttct tccttactta atataaatct ttctctatca tgtaatttat
     1561 ttagtttttc atcatttttt tgttctaatt cataagcttc tttttttatt gtgttagctt
     1621 tcgacagttt agtgattcct ataattaata aaataagtcc aatcaataat aacattcaaa
     1681 actcctcatt tctttgatat aattattgta gagtttactc agaaatgagg aaagagtcca
     1741 tgtgaaagca tgggcttttt tatttttaat gttgtaatac taaatgttca agattatgag
     1801 gaagccctaa atattcaata atttggaact gactcatatc agataaatct tgttcatcta
     1861 ttgttctagt taacatttca attgcaaaca tattagcttc agcttctata ccataaatca
     1921 tacaactaag gttgtttgct ctaaaagctg ctgtgcaaat acctttatgg agtctaacgt
     1981 gggctatttc atgccataat acaaatcttt tcatagaagg tgataggttt tgattaatta
     2041 atatcacata acatctttta ttgtgtatcg tttgtcctaa tgttttattc cctaaatcag
     2101 tttcaattaa atctatatca gaatatttga ttattttata tggattaatc gcatcagatc
     2161 caaactctac aaataatttt tctatttctt ctaatatcca gtatttgttt aaatgcatac
     2221 caaagcccac ctattgacga tattttttag gagtgaattt tttctttgct ttaactttgg
     2281 ttaaggcaag tgcaagttga atggcatcgt ataatttttc cttatcttca tcgtctaaag
     2341 gttcaccata ataatttgtt tcagcatctg aattcaagcc ttcaagtaat ttttcagctt
     2401 gtaaagcaat atcttgttta tctttttctg ttaaatcata ataatggcgt ttatcagtac
     2461 gtcctaaaag ataatcagtt gatacattaa agtaatctgc aactttttgt attttatctc
     2521 cagatggagt acttttattc catctagaca tgcttccatt tccgaaattt aactttcttt
     2581 caagttctgc caacgtcatt gaacgttctt ttaataagat ttttatacgg tctaatagca
     2641 taggatatgc attcctttca aaataccgac aaaaaaagta tttttctgca aattatctat
     2701 ttacaaatag agaatttgca gttatactag ttttgtaagc aagatattta cggcatacaa
     2761 agactaaaag caataaacta atagtctccc cagacatcag taagctcgct ttttgtgcgc
     2821 tgtattttgt atgcttacat aatagcatat ttgcagtttt tgtcaaactt attagacaaa
     2881 aatattagaa aatattcgat aagggggtat gaagcatgac tttaaaacag caagtattaa
     2941 ttgctttagt aaagaaaggt tggagtcaaa gagagttagc tcgacgaatg ggtatatcaa
     3001 ttacttattt acgagatatc ttcatcgaaa aaagaaaacc taaggaacgt ttaaaacaaa
     3061 tcgaagaact tttggatatc aagttagaag tagattctaa tgatcagcca tcagaacagg
     3121 aggcgtaaaa atggtacaac gtttgagcaa agcggagttt aaagagaaat ataagtattc
     3181 tgaaagtact tatcaaagaa gaatgaagga atttaaaaaa tcacgtttta gtgaggggta
     3241 taacgcagtt acttccaaag aaataacaat tgatgtcgat ttatatgaac agtttagaac
     3301 atggcgttct aaaaatcgat ttagagttag agctgtttgt taatagctaa aaaatctaaa
     3361 ttcaccactt ctgaaaagag gagttgctgc tctcctttca agtttgccaa agtccacgtt
     3421 ttttcatttt ggcaactcct cttcttggag gtggtgacgg cgagagacga aggagggatg
     3481 acatgaagga agaatcaaaa atagggattt tgaatgagaa caattacaca agattaatta
     3541 ttgaaacaga agaagggaaa aaagtagcag aagttacatt gaccgatgca actcctgctg
     3601 acggttatcg tattcgatta actcctaaat atgattaacc ttttggtggg tgtggatcgt
     3661 ttccgtgact atcacggctt tgaatttttc catctctgcc atgaataaat agctcagatc
     3721 cttgattttt agaaatctct ctagcgattt ttactgcttc tgattttgta ttagtatgtg
     3781 cagtcgcttt agaatttcca gcacctttga cattccaacc accattggaa ttaggtacaa
     3841 catgttgatt ttttcccata gttttcacct ccatttctat agaaatattt tatcaaatag
     3901 aaatggagaa attcaataaa cgaaaggaga agtgatatgg catatacctt gcaacaagaa
     3961 catcaaattc tcggtttgat taaacaacgc aggaaacaat tacaagatga ccgtgcagcg
     4021 cttagaaaag ccgatgagct atcagatagg caagctgaac taattgcttc tgaacttgag
     4081 gatttgagaa tgctagaaat aaaaaatagg gagattagat tatgaagaag acagacacac
     4141 tttttatagg attcattttg gggttattag tgattgtagc gcaccaaagt attatcgggg
     4201 gaagcttgtt cgcagcattg atggttttaa tcaatctgct tgattcaaaa gaaaggagca
     4261 actatggcac gagaagaagc gctaaaaatc ggtaaagtga ttgctgataa tcggtgggga
     4321 aatagccgcc ctattatttt aagcaagcaa catatcgaaa agcaaaaagc atggcaacaa
     4381 ataaaaaagt gactctgcca gccagcaaag agtcacatac aaatttataa aactaggaga
     4441 attatatcac atgaataaga aaattgaaaa tcttatcgaa gaattgaaac gtgaatgcca
     4501 aaagcaaggt gtttctatta tttgtacagc ccaaaaagaa ggggaactta aaagtctcgt
     4561 ttatggtgag acaacagaaa ttttactttg tcttgctatg caagaagaac atttagatga
     4621 aaatctccca ttgtcagcac atattatgcg tagaattgct gtggatgctt acgaacaagc
     4681 gaaaaatgaa gaagaaaatc aaccttcaaa tcatactttt gttattaata acaaggaaga
     4741 tttagctgat gtgatgactc gtattctcaa gggggaattt caatgatgaa tgaaaaaaca
     4801 attgatctac tcattatttt atttttcacg ttagtgattg tagcgaaaag ttatgtaatc
     4861 tttggcttgt tattcgcaat taccatgctt gtgctttgcg tgttagcaag cagaaaagag
     4921 gttaaacatg aacgagttag caaatcttga caactattta actgatcctg attatgctaa
     4981 accaccatat gaagcaccaa ttgatgagga ggatgaagat gagtaaatct accttagaaa
     5041 tgagccatca agaatggctt gaagaccgta agaaaggcat cggaggttcg gatgttggaa
     5101 caattttagg attgaataaa tggaaatctc cttatcaact atggctagaa aaaacaggac
     5161 aagtagtact tgaagaatca ggaagtgagc ccgcttattg gggtaatgtt ttagaagaag
     5221 tggttgctaa agagtttcag gaacgtacag gcaaaaaagt acgcagaaga aaccaagtat
     5281 ttgaacatcc attacatcca tttttaagag caaatattga tcgggaagta gtgggagaaa
     5341 atgccattct ggaatgcaaa acagccaatc aatttctcgg taaagaatgg gaaggcgaag
     5401 aggtaccgct tagctatctc tgtcaagttc aacattacat gaacgttcta aacaaagact
     5461 attgttacat cgctgtctta atcggtggtc aaaaatttat ctggaagcgg attgaacgag
     5521 atcaagagct gatcgataca atcactgaac aattagtaga gttttgggaa acaaacgttc
     5581 ttggaggtat cgagccaatt attgatggta gtcaagcgac agctgatttc ttgaaagaga
     5641 agtatgcaga tgttgaagac gttcaaacga cgttaccaat tcatttcgat gaactagttg
     5701 aacagaaaaa cgaactcaaa cggactaaga aagaaattga atcagctatc cgtcaagtag
     5761 acaacgagat tatcagcgag ctaggtaaac gtgaagccag tatcggtatc actcaaaaaa
     5821 acatcatcag ttggaaattt gtccgtacga gacgtatgaa ctcgaagaaa ctagcagaga
     5881 aatatccaga tgtcgcaaat gatgaagaga tttataacgt tactgaatca agaaggctaa
     5941 ccgaaaggga gatcaaataa tatggcaaca aatgaatcgt taaaaaatca attggcagca
     6001 aagccacaga aacaagttgc accaggtcaa ttaggactta aagctttgat gaatacacca
     6061 acaatgagaa agaagttcga agaggtgctt catgacaatg ctaatgcttt tatgtcgaat
     6121 gttatgactc ttgtatctaa tgacagttat cttgcagata gtgaaccgat gtctatcatg
     6181 agtggtgcgt taactgctgc aacattaaat cttgggctag ataagaattt aggttatgca
     6241 tatttagttc cattcaatag taaaaacaag caaacaggaa aatgggaaaa gaaagctcaa
     6301 ttcatgcttg gctataaagg atatatccaa ttagcccaac gatcaggtaa atacaaagca
     6361 ttaaatgtga tcgaagttta cgaaggagaa ctaaaaagct ggaaccgact gacagaagag
     6421 tttgagtttg atccaaatgg tagaacgtct gatgaagtca ttggatatgt tggctatttc
     6481 gagttactga atggattcaa aaaaactgtc tattggacca aacaagaaat cgaagctcat
     6541 cggattgcta acaataaaga tcgcgataag acaaagttaa gtggtgtgtg ggcatctgat
     6601 tacaatgcaa tggcacgaaa aactgttttg agaaatcttc tttctaaatg gggaatcttg
     6661 tccatcgaaa tgcaagaagc caccacatcg gatgagagag tccaaagggt tcaagaagac
     6721 ggcagcatta ttgctgaaac agaagttgaa gaagatattc ctgaaagaaa agaagcagag
     6781 gttatatctg aagaaaatga agatgtacaa actggattat ttgatgcatc taatccgccg
     6841 ttaaacaaat aatgagggag ctttctccct tgcttttcta gaggagaaat acgaatgaac
     6901 acaggatatg tcaaattaca tcgaaaagtg atgaattcat tcgtttggac caatccttat
     6961 atgtataaat tatggaactt gtgtttgatg aaagcgagtc atgagaatcg caagatactt
     7021 tttaatggaa aagagataca agtgaacagc ggagaattcg tcacagggcg cgatgctatc
     7081 acatctgaga tgaacgaagg cgtgaaacgt gaacatcaag tgaacagcgg ttctgtatgg
     7141 agatggctaa aacaatttga aaaaaacgga atgttgaaca tcaaatcaac tacgaaatac
     7201 agcgttatat caataaaaaa ctggtctttg taccaaagca gtgaacaaca aatgaacatc
     7261 aaacgcacaa caagtgaaca acaagtgcac acaaacaaga atgaaaagaa ttataagaat
     7321 gaaaagaata ataaaacatc atcgtcgcaa ccacgcaaaa agcgtgttta cgacaccgac
     7381 tcagtttact acattctcgc ggaggagtta ttcaaacaga tttgtcagaa tcaggaaatc
     7441 aaaaagccga atctgcaaag ttgggcagat gacattcgga aaatgatcga gatcgacaag
     7501 cggaccgaaa accaggtacg gggaatgatt gaatggagtc agcacaatgt gttttgggca
     7561 tcgaatattt tatctgctaa aaaattgcga gaaaaatacg acacaatggc agcgcaagca
     7621 aatcgtgatt ataaaacaaa acaaactaaa acgcttgaat acgagaaatt tagcacagat
     7681 gagttgccta tttgagagga ggcgtaggca tggaaactgt tggggaaatc atggaaaagc
     7741 tgatacagaa agtgcttgtc caacgtggcg aatgtcctga atgcggacag cctttgtatg
     7801 gatggcgtac gaagaaccct gatggctcag aacgttgtaa gccaacctgt atgcaatgtg
     7861 ggtataaagc attgcgagtt caggaagact tacaaacaga acgaatttac aacgagagcc
     7921 tgaaagcaag agcaatcaat tttttcaaag gtggttctgt tgtgcctaat caagcgttgt
     7981 ttgattgcac attgcagaat tatcaaattg tcgatcaaga aacaagacaa gcggttgaag
     8041 taaccaaacg ctttgttaat tcagtcttgt taggaaatcc aagtcacctt gttttaactg
     8101 gaaaacaagg aacaggtaaa agtcacctag cgatggcagc ggcttgggaa gtcttgaagc
     8161 gatcaaacta cgataaaaaa atcttattta tcgggttaca ggaaatgctg gatcaaatca
     8221 aattttcgta caacaatcct gaactcagaa aaacgattga gggatcgttg attgcagata
     8281 tcaaaacagc ggatttagtc atcattgatg atatcggttc agaactagga aaagatgcat
     8341 cagatagtcg agcgtttggc ataaacacgc taaattcgtt cttggatgca cgacagaacc
     8401 tagcaacgat tatcacaacg aacttgcttg gtgaagaact gaaaaaagct tacggtacga
     8461 gaacaatatc aagaatgttt gtcaactctg atggatttac gatggtattt tctcaaacag
     8521 cagacaagcg cataaaacca gtcaaaggag cgatcgcatg aatttgacta aacaagaaaa
     8581 aagaatctgt cgtcagataa tgttgcaaat tataggtgat cgtgacatgg acgaattatc
     8641 tcaacaagag agtcgaatgg ttcggaagat aagtaagcta attatttatc gggatacaga
     8701 aagatttctc actgttttag gtaacgtcta cgagaatcca gagttattgg aggtagagta
     8761 attgccaaaa atattagatg catgttgcgg tagtagattg ttctggtttg ataagaacaa
     8821 ttctgacgta acatttatgg attgtcgtca gcagtatgaa gaattatcaa caggtcatgt
     8881 gatcaatgtt gatccagatg tggtcgcaga ctttcgcgat atgccttttg aagatgatgt
     8941 gttcgatatg gttgtatttg acccgccaca cttaagacat gtcggcgaaa actcatggct
     9001 tgccaaaaaa tatggaaaat tagacgaact ttggccagaa gacatcaggc aaggttttgc
     9061 agagtgtatg cgagttttac gaccttcagg aacattaatt tttaagtgga acgaggaaca
     9121 gataccactt tctgatgtgt tagaggctat tggagaacag ccattgtttg gtaataaacg
     9181 aagcaaaaca cattggcttg tgtttatgaa aaaattcagc taacggagga aacaaaggag
     9241 gaaaagtaat gaagattgta ttaaataaat gttttggtgg ctttgggttg agccatgaag
     9301 caaaaatgga aatttttaaa agaaaaaata ttgaagtatt cccatacatc aataacttta
     9361 gctatgactc agatgatgaa tacacgagat acacaggcca aaagctaggt tcgatggatt
     9421 ttatctacta tttcaaaaaa gacccgaaaa tcgacaaagt tacaggaatt tatagcgaaa
     9481 ttgaccgact gtacggaatt gctgatgatt ccagtttttc aagtgattca aacagaggtg
     9541 ataaggacct cgttgctgtt gtcgaaaaac taggaagtga ggccagtgga ccatatgcaa
     9601 gtcttaaagt cgttgatata cccgatgggg ctgagtggga aataagtgat tatgacggcg
     9661 ttgaaactgc acattatggt tttcaaacag gtagtattta gtccactaat gacaactaaa
     9721 gaggaggcag aaggatgagt gaagatgaat actttgaaca gtagtctatt aacgacagag
     9781 atttttaact ggaaaggggt gtagtcatgg acgcatcgga agtagtaaat aaaattaata
     9841 gtctcaaaga ggttttcgga gacgtcgaag tagtgataaa cgcacaaggc tatgacaacg
     9901 aattttttaa gcagattacc gacattagca ttcagcaagg aatggaagac gaagacggaa
     9961 actttatcga tgaagtagcc attttagtga cttgcgagta agtaattgac agctaaagag
    10021 gaggaagcgg aatgaaacta aaagacggat tttactccag cggccacggc atcggcggtt
    10081 taatgctaga tatgccgaca aagaatccta aaacacgtaa gaaaccaaaa ttcaaagtcg
    10141 gtgacatggt tcgctgcgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac
    10201 acgtctataa tcactcagca atcattcgca ttgaaaacac gatggaatgt gataagtggt
    10261 tagcgaaaag caaagagaat ttagcagtgg tgagattggt ggacatggaa gttataaaat
    10321 cttaattttg cttttttaca actatcctac aacaatgagc gttattccac aactggaagg
    10381 agaagaggca attgtgaaca ttttagagaa tatagatatt aagcaaacaa ggaaaaatgc
    10441 tagacgatta ctaaaaagat acagaaattt agaacgtttg gtcggaccag tgaaaataga
    10501 cttttccgtg atgactgtta ccaaaaattt gaaattcaca attgacagtc aaaacgaaga
    10561 aatcattgaa gcgataagta ctcgagattc ggttatcgaa gcattaacac ggctaagtag
    10621 aatccatttc caggtacttt attatagtta ttgttttcct aataagatgt caatgtatca
    10681 aataggagaa aaattaggat attccgatag aactatcgag agaatgaagg cagtagcttt
    10741 ggttgaattt gctgaggcgt ataaatcagg agaactcatt tcacgtacaa aataaaaaaa
    10801 gtcggattcc tccgactatg aataatattt ccgacataag tattatatca taattggggg
    10861 aatcagagga tggtactttt cgatgtaaag aaatatgaga caccaagcgc gaaagatgtt
    10921 gatatggaac gcacaaaaca taatgtcgct gtgtttcttt cagcatatct atcagctaga
    10981 tgtagagtag ggcaacatcg agaaccaaaa gtgacagcat cttattcctt ggttccacct
    11041 tctacagctg atcatatatt tgaagcagaa agaatgctga tcgataaaga agaagcacag
    11101 gaagaatttg agtatctgca taaattgttt gttcgaggct attcagcgat acagcatccg
    11161 cataagcctg atgtgactga aaggcgcaag aagatattct atgatcgtta tatcaatggt
    11221 ctgcccattt atgtaactgc tcaaaggaat aatactagcg aagaatcggt taaagtagaa
    11281 tcaaacagaa ttatcatcca atttgcttca tcgttagaac tggttgcttt caagtagcca
    11341 gtttttacac tttttatacc cttttattac caatttggtt tccattttat acctttttta
    11401 taccaatcac ttacctattc aacgttgtat tatgatagtg tcgaaaaatt aggaaacagg
    11461 atcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa gcttcgatag
    11521 gcagcaacgg aaatattaag aataaggatg tgaatttcaa ctccttctaa attgttctta
    11581 ttatctatca tccgttgctg tctattgttt ttttaattat tcacacgata actaaaggta
    11641 agtgaagaga aatgattcca ttaataattt caatttttgc gctttgtctt aatgtatata
    11701 tgattggatt taaaaatggg caaaataaaa aatagtagca gccaagaata attttatagt
    11761 gtcactgtgg cggaaagggt agacgctaag catgtgtgct aggtcaatgc ttcggcaacc
    11821 atgcaaggtt cgattccttg ccagtgactt tggcaaccga ggcatcggcg gtttaaaaat
    11881 ataggggtgc gcaatttcgt acgcgttttg tgcatcgtgt aggttgctat tacataactg
    11941 gttaggttag attgagattt gggattcggt acaaatgaat cgtcaaatgt ctcaagcaca
    12001 ggatcggaaa cgtccctgcc tgtgcattac atattagatc actctttgag tggtcttttt
    12061 ctttacataa aggaggctac ataatgagaa actactggta tgtatcgcta actaatgaat
    12121 atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtacaa atcaaaggga
    12181 agtactctat tgtcgaaatg gggagagaag cagaaccatg tgagattgat gcgtgcaagc
    12241 tcgtttattg cggtcatggt ttctatgatg aaccaaacat tcaaaataat attaacaaga
    12301 atttgaggga ttagaatgca agaaatggct taaaaatctc caatcaggac aaatcaagtt
    12361 agacaaagtt tccgatttaa agatattgat tgaagctgat ttaatgctaa aagatattga
    12421 aaattagaaa acaaaactca acctaaacag aatgtgaggt ggtgtgtatt gaatggcaag
    12481 acaacgtgat ccaagacgtg atgaagccaa aagaatttgg ttagaatcca acggagaaaa
    12541 gcagttaaag gaaattgcat ctgaattaaa tgtttcagat tctcaggtta gaaaatggaa
    12601 atcgcaagac aaatggagcg ctgaattgat tagtaacgtt accaatggca aaagtaacgt
    12661 tactaatcaa ggtggcgctc ctattggtaa tcaaaatgct aaaggtaaca aaggaaatag
    12721 ccgagcttct ccgccagtgg gtaataaaaa cgctttgaaa acaggtgagt atgaaacaat
    12781 attttttgac acgttaaccg ataatgagaa ggacatctat tctagtttga atgatgatcc
    12841 ttcttttgct ttgtccgaag aaatacgact tcttaaaata agacaacttc gaatgatgaa
    12901 aagaatccaa caagctgaag ctggactaaa tgatgaagaa gtcgaacgat tgcagcagct
    12961 aagaaagatt aaaactccga ttgaaaaaaa tggtaaaaag ctagaaatca agcgtgaggt
    13021 tatgcaagat gtgcagatta gcagaaaaaa acatcgcaaa attgatgata ttctttcaat
    13081 tgaagattca ttgactcgga ttagcaacca gttagctaaa gccatcaagc aaatgaatga
    13141 actttatatg aatgaataca gaactgattt aattaaagct cagactgata agatccaagc
    13201 tgagacaaat gaaattggcg gaaataattc aggtgaagaa atagaagaat ggaaacaggc
    13261 agttttaaat gccgcaaaca aacgggcggt gaaagaaaat gaataatgaa tttattcctt
    13321 ttgctgatat tggttctgcc attgattatt actatgataa accagtagct ttttgccaag
    13381 atattttgca tttgaatcct gatgaatggc aagaaaatgt tttaaatgat ttagctgaat
    13441 tttcaaaggt ttctgttcgt tctggtcaag gagttggaaa aacagcatta gaagcaggag
    13501 caatactttg gttcttaacg tgtcgaccct acgctaaagt aatagcaaca gctccgacaa
    13561 tgaagcaact ttacgatgta ctttgggcag aggtagctaa atggttaaat gatagcttga
    13621 tcaaaaactt actgaagtgg acaaagacca aaatttatat ggttggtgat tcagagcgtt
    13681 ggtttgctac ggctagaaca gcgactaaac cagaaagtat gcaaggtttt cacgaggacc
    13741 atatgttgat tgtggtagat gaagcttctg gtgtgtctga tccaattatg gaagctattc
    13801 ttggtacgct atcaggtttt gataataagc tgttgatgtg tggaaacccc aataatattg
    13861 aaggtgtctt ttatgattcc cataattcag accgtgataa atacagagtt cataaagtat
    13921 caagctatga tagtaaacgt acaaacaaag acaatataga aatgattctt aaaaaatatg
    13981 gaaaagaaag tgatgttgct cgtgtccgta tttttggaga atttccgaaa ggtgcgttgg
    14041 attcatttat cagtcttgaa acagttgaat tggctacaga aaaacaaatt agtgattctt
    14101 tagtcaataa aacaaaggtt gctcatattg gtgtcgacgt agctcgatat ggtgatgatt
    14161 ctacgattct ctttcctaga attgctacca gggcattgga gtatgagaag tattcaaaac
    14221 gtagcaccat ggaaacaaca ggatatgtca tcaacatggc caagaatcta atgagtcaat
    14281 atccgagtat tgataaagtg atgattaaag tcgatgacac tggtgtcgga ggtggtgtaa
    14341 ccgaccgcct agaagaactt atagaagaca aacattatcc ttttgaggtg tttggagtga
    14401 ataacggttc aacatcagaa gacgattttt acgataattt aggtactcaa ctatggggaa
    14461 acatcaagga aatgttagaa gaaaatatga cagcaaatct taacggagaa cagcctgtta
    14521 ttgaattgcc ttctgatagt tcgttaatca aagaattaag tactcgcaaa ttcaaaatga
    14581 caagtagaag tcgtatacgt ttagaaagta aagatgatat gaaaaagcga aatattggta
    14641 gtcccgatat tgctgacgca ctggctttag cgttttatga gccaccaagt cactatcaat
    14701 ttattcaatt ttaggaggtg agcttttgat tagtacagta ttatctttag agagatataa
    14761 gaagctacgt gttaaatatg caacacaaat tgaagatgga atgtttgatc cgaatggttt
    14821 tatagaagat atgaaaccat tttttgctga tcgcgaaaga aaatatctag cttatactag
    14881 cgaaaagaat gaaatagata atagaccaaa gcctaacact gatattgtaa aagttaataa
    14941 taaacttcat gctggtatgt attcaattgt cgtagaccaa gcagtcaatc attttactgg
    15001 tatacctatc aaatgggatt acgatgtatc ggaacaaaaa agaacactca ttcaaagatt
    15061 aaaagataaa ttcttaaaaa atgatataga acttccgaca gttccagaag cttttaacaa
    15121 attaacaagc aatcttgatt ccatgagatt cgcaatgctt gattctgaaa ctgcaacttt
    15181 tcaaggagct tgtggagtag ctttccgatt gctagagcct gtggaagaag atgatggttg
    15241 gaagttaaga gcaagtaata ttgaaccttg gagagcagaa agatatggaa atgccggaat
    15301 ctatatcaaa gaaaagtatg actcttacca gaaaaaattt tttcaagaaa tgaaagttat
    15361 gacaagaaat aaaattctga catatgcttg ctatggtgat ttgaattttg ttacaagcgg
    15421 aacgttcaaa aaaattgatg agaccgataa ccctttgggg acgattacct tggcagaatt
    15481 taaaaataat acgaatcgtt attgtgattt tgaagtagct gaggaaattg gagatgcaat
    15541 tgatcgtgct ttgtctgatc aacaaaacga aatcgaacaa tttaagcttg cgtacatgct
    15601 tgttactggc acaacaatga gcaaaggcac tgctaaagaa atgatgaatc agctagggat
    15661 tatcaactta aaagccccta ctgcaaaagc tgaatatgtc acaaaaaatt tagcaaaaga
    15721 tttcaatgaa tatcatatgg atctattgaa aaaacagttt tacaccattt gtaaagcaat
    15781 cgattttaac gatgaggtgt ttaaatcaaa cagttctgga gaagctcgca agtggcaaat
    15841 cattagccta gaagcgaaaa caaatactaa agagcaatat tttagagaag gattgaaaga
    15901 atgcgcagaa acaattgctg cttttcttaa gtttcatgac aaagtagaaa ttgagcctga
    15961 aaaaattatt tttactttct ctagatcttt gccaactgat ttaagttatt tagctgaagc
    16021 tttacctaaa cttgcaccat acgtatccaa acgaactatt cagagccaaa ttccatttgt
    16081 aacagatgtt gattatgaaa atgaaatgat ggaattggaa agtggaagtg cttatccaga
    16141 tagtgaatac aattttggcg gaggtggcaa tagtgacgat agaaacgaag tattggacca
    16201 aacgtcgaga actggaggat caagcaaggc tcaaacaaga aaatcagaca cttaaaaaat
    16261 taactagtgt atttcctgaa gcactgaaag agatacaagc aaaactatta tcacaagctg
    16321 acttacacaa tatcacttat ccagaaatga tggagtttta cagtacaagt aatcagaaaa
    16381 aatatcgtga atatgtggaa aaaaattata agtcattaaa aatgtatgat gcaaaataca
    16441 aagagtttat cgatgaattt tttccaccat ttgactatgc aaaagtcaat cgcttattac
    16501 aaatacgatc agatgtattt aaaattcttg cagaatatgc gatggatgca gatgtgaatc
    16561 aatatttttc tgatcgctta gaggaaattc ttcaaagaac atattcttct aatgctaatg
    16621 tttttgttca acttttaaac gttgatatac caaattattt accagaaaat gaaattaaac
    16681 attatttgaa ctatccatgg tcaggaaaga cattttcaag aagactttgg ggaaatattt
    16741 catcgcttga acagaaactc tctaatgcta ttgtaaaaag tgttgctagt ggagaaggcg
    16801 ttatacacgc attaaacacc atgagattag actcagaaat ttgtgacatg tttaagttag
    16861 aagaatcaaa gtataacaaa gcgatagaaa atctcgttcg aacggagtat gcaaaatttg
    16921 cacaagatgg tattgaaaaa tcatatttag aaacaggtat tgaggaatac aacgtattga
    16981 ctgcaaaaga tgagagagtt tgccggattt gtggaggaaa ggcaagtaag aatccctata
    17041 aactgaaaga tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt tgtagatgta
    17101 cggatgttcc taatttacca aaattaggaa aggatattga tgaagaatat gaccgtttat
    17161 ttggcgattt attagatgag tttgcacatg attcttttgg aattaatttg aaacggagga
    17221 agtagaatga aagatttttt tgaagcagta ctaacaatta atgtaaatgc tgatattgca
    17281 gaagcctaca aaacagctat tgagtctgag aaccatccta atggcttgag agaccattgg
    17341 aatggcaatt atgcctacgt ggttattggc gatcaaactg ttaattatca agataatact
    17401 ccagttgata agaataccgt taatttaacg attcaattat tatctcattc attaccaaat
    17461 ttaaaagaaa cagttgattg gtatgaaaag atgggatgta ttgttgttag aactgactac
    17521 aaagaaggaa agtctagtaa ttaggctttt ttattttgtc cgaaatgacg ttaaactagc
    17581 gcaatactgg gcttggttga atggtggggc gcaactatta aaactcaaag caatgcgggg
    17641 cgtgaaaacg aatcgtgggg cgaaaggaga atgagcatga aacacaaatc attaatgcca
    17701 atgaatttgc aatactttgc cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa
    17761 tcttttgtgg aatccaatga agaagctcaa aaatttattc agtcacaatc tcaaagtgtt
    17821 gccgacaaac aattggaagc ttggaaacaa aataacttag ataaaattaa acaggatacc
    17881 atcaaggaat atgaggaatc taagaaaaac aagtcacctg agcaaattca actggaaaaa
    17941 ctacaagctg aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa taaagcattt
    18001 gttgcagaac aaattgctgg attagaacta gatggagagt taaaagagtc tatttctcaa
    18061 tttatgctaa ataatcttgt tagttcggat acagatttca ctaagaatgc tgttgaaggt
    18121 tttacaagtg ttttgaatgc aattaatgag aaacatgcag atgcattaaa agaactacaa
    18181 atgaagtctg catttggtgg aactcaacaa tcgaataacc aggttcagca gaacaatgaa
    18241 acattaacaa atccagaaga acaattagga caaattcttc aacaatttaa ctaggagagt
    18301 gaaaaattat gaaaaaaaca tctttaaata atttagagta tttggatatt tcaccagcga
    18361 ttaatgctat gcaagtacca aatacacctt ttttaagcta tttacttggt gctggaaaaa
    18421 cagagccagc aaactcgaca gaaattaaat ggcgtgaata tgatatcaac aacgatgatt
    18481 cttctgaaaa acttgagggc ggagaatatc cagatgctga atcaggtcga acttggttta
    18541 acaactatac tgaaattttt agaaaatcaa cctctgtatc tggtacatta gatgctatta
    18601 atgtgaatgg agtcggaaat gaattaacta atcaagtagc actacgtggt atggaaatga
    18661 aaattgattt gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag
    18721 gtcgtcgaat gaatgggatt ttgaacttga tcaattcagc aaataaggca gaaacagcca
    18781 ctgcgggtgc agtaacaaga aaagatatcg atactttatt taaattgatg tatgaaaaag
    18841 gttatatggg agaaaaacta tgcttgattt ctccagatat gcaggagtta atgactgatg
    18901 agttagatgg aaaatcaaca aaaattgttc agttcgggga aagagtaact tttggattgc
    18961 aaattggaaa tatcgtgtct aattacggta caggtattgc tctactagaa ccatcattgc
    19021 caaaaggaac aattgccgca atcgatacta attatgtgaa actacgtcca ttacgtgaat
    19081 ggagagcaga agaacttgca aaaacaacgg attctagacg tatcggtctt gtaggtgaat
    19141 actctcttga atacaatgct tcaaactctg gggcaatttt aaatttaaag tctgaataaa
    19201 agggagttag tactcccttt tttgatagga ggaattacag tggtaaaaaa agatgaaact
    19261 aaaaaagatg aagtcgtgaa atatagagta ggtaaaacta aaaattttgt tgggtttgtt
    19321 catcctaaaa ctcgtagatt tatcacagca gattcaaata acgaatttat catttctata
    19381 gatgataaag aagcaattgc aattttggaa gatgcaattg atgttaatga aatttaggaa
    19441 gtgatctgat ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa gaagattttc
    19501 cagatttgag tgaagaacgc ataactaatt tattagaaat aattttgcta gaaattgaat
    19561 catacaacac ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg attagtgaag
    19621 tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt agacgtggcg
    19681 atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt ctaggctatg
    19741 gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa tgaatgaagc
    19801 agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga cggacattga
    19861 agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata atcctatttc
    19921 ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg gagagatggt
    19981 aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac taaaaaaagg
    20041 agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat ttgctactaa
    20101 gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga aaaatgggta
    20161 atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa
    20221 ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc gttagtttag
    20281 ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt gtagtaaaag
    20341 ctagaaaact atcaagcggt cgaattgttg gagaagttat aaacaatgaa cattatttta
    20401 aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg aaattcatgt
    20461 taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt aaacaaatgg
    20521 caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt
    20581 agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt tatgcagtca
    20641 actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa tgtaggaatt
    20701 gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa ccctaataga
    20761 aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat tacaatcgat
    20821 gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt tcaacatatt
    20881 cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat gggagaaaaa
    20941 agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt ggaagatgag
    21001 acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa
    21061 ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca agatcaattt
    21121 acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca taaagtttta
    21181 tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg agaagatatt
    21241 gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata ccgttatacg
    21301 gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat tgaaggaagt
    21361 aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc aggacttgga
    21421 tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat cggaaattgg
    21481 tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac agaataatgt
    21541 caaaatatca aacaacgatt aaattaacga aaaaagacga agaaggtaaa tatgagcaag
    21601 tacaatttaa atctgctgaa tttttaccag gaacagttgt agaagatgct gcaggagtta
    21661 tggaagaaat gcaaacagcg actgataagc aatcagttaa aaaagcttta agtcgtgctt
    21721 attcatttat tgcagatact ttatttgagg gacaatttac tggtgaagat tattgcaaag
    21781 gaattgatgc tcgtgagatt gcctcattga caggaaaatt attgaagtct gttactgcag
    21841 gttttgatga aacttataca gagacgaaaa aaaagtaagt gaggcgctca agtcaccttc
    21901 atttaagtat tcgattactt accgagaatt agatataaag acgcaattac tcgaagcagg
    21961 ttggacgtta ccagaaattg aacgtactga cttggatgag ttaatgcgtc tttttgcttt
    22021 tagagatgcg gtaaaagagc atgaagatgt tgagtactat gataatttca ctcaatttta
    22081 ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga tgaatctggt
    22141 tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga aacaatcgaa
    22201 agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt tgctggaagt
    22261 tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat caagggttcc
    22321 ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa tttccaaaat
    22381 atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct tttatctcaa
    22441 ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca acttcttgct
    22501 tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa tgatggaatt
    22561 cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt atctcaaagt
    22621 ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc tcaacttgga
    22681 cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct gaaagaaggt
    22741 ttatctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga aatggatacc
    22801 aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac taagggaatt
    22861 aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga ggtaatagaa
    22921 ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat tattgataaa
    22981 gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc tacagcagta
    23041 tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt gaaattcatg
    23101 atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca acttaaaggt
    23161 ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg cgttggaaat
    23221 tcattaaaga ttatgggggc aatagctgct gctaatccat ttgttttaat tattggtgca
    23281 gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga gcttagaaac
    23341 aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata
    23401 aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa tgcagttgag
    23461 ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg gaaaggtatt
    23521 aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa tgcagtcgat
    23581 agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg gcaaagtata
    23641 aaagattcag ccagagaaaa atggaatgga atcaaaggtt ctattatgtc agttgctggt
    23701 ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct agagacctta
    23761 tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt
    23821 gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac aaaaaataat
    23881 atgattgctg tttggaataa tattaaagaa agtgccataa atatttggga atctataaag
    23941 aatatatttg taagttattt tacaaatatt tattttgctg cactcaatat ttggacaggg
    24001 ttcaagctta cattgataaa tatttggaat gaagtggtaa atcaagctaa atcgatttgg
    24061 ttaaacataa agtatttttt tattaatctt tggattgata taaaatattt tgcaattcag
    24121 aaatggattg aattgaagtt ttctattatt caaacttgga tcgatttaaa atataatgcc
    24181 attactactt ggaataatat taaacagttt ttcaaagata cttggaaaaa tattaaagat
    24241 acggcataca atacgtggat ttctataaaa aataccatga ttaatacatg gaataacatt
    24301 aaggactctt tctggaatat tgttactgga attgttaatt ctgctgaaaa tgcatggaca
    24361 aatctaaaaa atggtgtttc aaatgctatt aatcgggtaa aagaaatctt taattcgtta
    24421 agggaagtca atttatttga aattggcaag aacatcattg atggacttat aaatggtgta
    24481 aaagaaaagt ggaatgcttt gaaagatact atcaaaggta ttgcaggtaa tatcaaaaat
    24541 tggattaaag gagctttaga tattcattct ccatcaagat ggatgcgtga tatggttggt
    24601 aaaaatattg ttcaaggtat tatcgttggt attgataaag agcaaagtaa gttagatcaa
    24661 accatgacaa atttagtaaa aacaccgtct gttcaacctg tagtaaatgg aataagtcac
    24721 ctaccaatta cacaaacaaa gcaaaatata tctgctagtg aaacaaaaga aattcattta
    24781 catttaaatg tttatggaga tttacctgat tcaatggttc ggaagattac cacaaaaatt
    24841 aaaacagaat tgacaagaca aatgaaacga gatgctggtg cagtaggagg aacattatat
    24901 gcaacttaag agaggacaat tttttattaa tcaacattat tcttctgaat ttaatgtgta
    24961 tattcaaaac aggcctgctt ctgtttcagc tagtcgagta attgaattga gagagcgaga
    25021 aggaaatgat tctatcatta ttgataaagg ctattacaaa aatgtgacta gaaagattga
    25081 gtgttactat aaagcacctt caattgatat ggtgcaggag tgggaagatc gaatcactga
    25141 atggttagat atgtcgtctt atagtgattt tattctttac tatgatgaac aatatattta
    25201 tcaagctgta gtaatagaag gacctgaatt taaaggaaca agaaaaacag gaaatattgt
    25261 cccttttgaa tttacagtga gtattcgacc atttaaggaa aattacaatg gtagatttac
    25321 tattcgacaa acagaaactt ttgaaattta taacccagag aagtattctt caaaaccgct
    25381 tattaaattg agtggttctg gagatgcttc tttttatatt aataaggata aatatgattt
    25441 gaaatcgttg gacagagaat tatttataga ttctaaatta gaagaggctt atcgaaaatt
    25501 agatggtaat ttagaacatc aggatcaggt caccttattt ttagattttc catttttata
    25561 tcctggaaaa aatgaaatta aatggacgaa aaatattcat tcatttgaaa taatgcctag
    25621 gtggtggaga aaagtatgaa accaagaata tatagtccta ctgaaacaga tttctcaacg
    25681 aatggcttag gaattttaaa ggattgtaca agatgtggaa tatatgaagt agctaatgga
    25741 aaatatgaat tagagttgga ttatccttta ggaactagat ttgatgaata ttttgaaaat
    25801 gactatcaaa taaaagcaaa gccaaacgat caagaagagt atcatatctt ttttattgat
    25861 gataaagata tagatacttt tttaaataca gtaactattt atgctcagag tcgtacaaat
    25921 cgacttggaa gacgggtagt cactcttgtg gaagtggact ctaaaactgg tcaagaagcg
    25981 atatcaatta ttgaaactaa aatggataaa aaatctgaca tacgacttta ttctgatatt
    26041 acggctgttt ctagcacaat ctttgaagcg agaaatgttt taaattgtat tgctggtgaa
    26101 caaggatcat tgcttcaata ttggggcgga gaaattaaac gtgaaccatt taagctttct
    26161 ttgttaaagc gaagaggtcg caataatgtt ggaacgattc ggtatggaaa agatatgtct
    26221 ggtttgaagg tcaaattaga ttggacaggt ataaaaacaa gaattattcc atatgctgat
    26281 cctcaaagtg aagtaggtac gactaatcga atttatggtt cgccagtaga tagtgcctat
    26341 attaataact atcctgatgt ctatacagaa catgttcagt ttacagaaga acaaggagta
    26401 aaagacatta agagcttaaa taaaatagct aaaaattact ttaaaacgat caatcctggc
    26461 tgtgataagc ctaaagtttc tattacggtt gaatttgata agttgactga tagtgaagaa
    26521 gcgaaagaat ttgcgaagat tagaaattat ggtctgtttg atacatttaa aatatatcat
    26581 aaaaaatata atatttatct tgaatcaaag gttagtggac tacaatacga ttctttatct
    26641 gaaaaaactt tgaaattaga agcaggagac attcaagttg ctttttatca gcaacaagct
    26701 gtaactattc aggataaatt aaaagattat gcaacgaata attatatgag tgattttaat
    26761 gattatgttt cttcaatgat tacaggtcaa ggcagtgcag gaggatatgt agttttatgg
    26821 ccaaaagaaa aaccttccaa catctttatt atggataatc cagatttaga aaaggcaaga
    26881 gaagtactaa gaatgaataa aaatgggatt gctttttcta agaaaggatg gcgggggcct
    26941 tttaattcag cttggacatt agatagtata tttaatgcca attttataca aactggatta
    27001 atcaaagcag atatctttca aaattctttt aacaagactg gagatgtatt gaaattagtt
    27061 aatggactac tccaaatttg gaacaacaaa aagaaaataa tggagttaac taaaaaagga
    27121 atggaatttt ggaattctaa gggttcaatt ggaacgattg gaacaactga ttctgttggt
    27181 aatccttttc ctggggcttc tactccaacc cctattgaag ataattcttt agttattcgt
    27241 acaaatggag acggcaaata tattttgatt tctcctaaag taggaaaagg attagtttta
    27301 ttaggaaatg gtaaagcaat ttattttgga gacttagatg tacaaggaaa acttacagtt
    27361 aaaggaaaag aaataattcc tggacaaggt ggtggtccat cgggaggtgg agaatctact
    27421 ggtgggtatc ctagtgaatt aaaaacagat gcagaaaaga gagcttggag aat
//
LOCUS       DASJST010000025        25743 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJST010000025 DASJST010000000
VERSION     DASJST010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.025"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            35..667
                     /locus_tag="U4B05_000827"
     CDS             35..667
                     /locus_tag="U4B05_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2049982.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(801..926)
                     /locus_tag="U4B05_000828"
     CDS             complement(801..926)
                     /locus_tag="U4B05_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2049983.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1060..1953)
                     /locus_tag="U4B05_000829"
     CDS             complement(1060..1953)
                     /locus_tag="U4B05_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN2049984.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2135..3061)
                     /locus_tag="U4B05_000830"
     CDS             complement(2135..3061)
                     /locus_tag="U4B05_000830"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN2049985.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3146..3907)
                     /gene="pflA"
                     /locus_tag="U4B05_000831"
     CDS             complement(3146..3907)
                     /gene="pflA"
                     /locus_tag="U4B05_000831"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN2049986.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4151..6382)
                     /gene="pflB"
                     /locus_tag="U4B05_000832"
     CDS             complement(4151..6382)
                     /gene="pflB"
                     /locus_tag="U4B05_000832"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN2049987.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6659..9109)
                     /gene="parC"
                     /locus_tag="U4B05_000833"
     CDS             complement(6659..9109)
                     /gene="parC"
                     /locus_tag="U4B05_000833"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN2049988.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9121..11166)
                     /gene="parE"
                     /locus_tag="U4B05_000834"
     CDS             complement(9121..11166)
                     /gene="parE"
                     /locus_tag="U4B05_000834"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN2049989.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11332..11766)
                     /locus_tag="U4B05_000835"
     CDS             complement(11332..11766)
                     /locus_tag="U4B05_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN2049990.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11893..12531
                     /gene="plsY"
                     /locus_tag="U4B05_000836"
     CDS             11893..12531
                     /gene="plsY"
                     /locus_tag="U4B05_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN2049991.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12663..13538)
                     /locus_tag="U4B05_000837"
     CDS             complement(12663..13538)
                     /locus_tag="U4B05_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN2049992.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13620..14411)
                     /gene="codY"
                     /locus_tag="U4B05_000838"
     CDS             complement(13620..14411)
                     /gene="codY"
                     /locus_tag="U4B05_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN2049993.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14429..15829)
                     /gene="hslU"
                     /locus_tag="U4B05_000839"
     CDS             complement(14429..15829)
                     /gene="hslU"
                     /locus_tag="U4B05_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN2049994.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15843..16391)
                     /gene="hslV"
                     /locus_tag="U4B05_000840"
     CDS             complement(15843..16391)
                     /gene="hslV"
                     /locus_tag="U4B05_000840"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN2049995.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16428..17333)
                     /gene="xerC"
                     /locus_tag="U4B05_000841"
     CDS             complement(16428..17333)
                     /gene="xerC"
                     /locus_tag="U4B05_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN2049996.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17531..18907)
                     /gene="trmFO"
                     /locus_tag="U4B05_000842"
     CDS             complement(17531..18907)
                     /gene="trmFO"
                     /locus_tag="U4B05_000842"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN2049997.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18876..20954)
                     /gene="topA"
                     /locus_tag="U4B05_000843"
     CDS             complement(18876..20954)
                     /gene="topA"
                     /locus_tag="U4B05_000843"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN2049998.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21076..21933)
                     /gene="dprA"
                     /locus_tag="U4B05_000844"
     CDS             complement(21076..21933)
                     /gene="dprA"
                     /locus_tag="U4B05_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN2049999.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21989..22756)
                     /locus_tag="U4B05_000845"
     CDS             complement(21989..22756)
                     /locus_tag="U4B05_000845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN2050000.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22749..23609)
                     /gene="ylqF"
                     /locus_tag="U4B05_000846"
     CDS             complement(22749..23609)
                     /gene="ylqF"
                     /locus_tag="U4B05_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN2050001.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23869..24267
                     /locus_tag="U4B05_000847"
     CDS             23869..24267
                     /locus_tag="U4B05_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2050002.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24546..25046
                     /locus_tag="U4B05_000848"
     CDS             24546..25046
                     /locus_tag="U4B05_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2050003.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(25129..25680)
                     /gene="lepB"
                     /locus_tag="U4B05_000849"
     CDS             complement(25129..25680)
                     /gene="lepB"
                     /locus_tag="U4B05_000849"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2050004.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         6795 a         5452 c         4422 g         9074 t
ORIGIN      
        1 ctttatatac ttatcaattt aatttggaga tattatggaa attcaacgta gaaccactaa
       61 ttttttacat ttaggctctt tttttgcaac tttgacgatc ttattttttc tatgttattc
      121 ataccttaag acctcacatg cgccttctag tgcattaggc tggttgcttt tggatcattc
      181 tgatatcaca gaagccgaag aaaatccgtt ttttttgatt ttgtcttctc tttgcggctt
      241 actgttttct gtttatttcg gtgcctattt ttttcatcga catcctggta cgttaaacga
      301 agcttcaagc gtattgattg catttaaaac gttttctttt ttagcaattc ttttttattc
      361 gtttaatcac aatacgcttt tcttctttat catgaatggt ttagtcgtga ttttatccat
      421 ttttacttat tttatttttt cactcatttt tagccaattg aaatgtccgc tcttctttcg
      481 attagttcct gtcacctgtt ttatttacag cttgttttcc ttttttgtcc ttgctttaat
      541 gccactgacc aacccttcga cgatccaagt cgcaggaaat ctgttggata tgctcttcat
      601 cgtcagtcta ggtgtattta tgtggcaaag aaaaaacaaa caaaataaaa agcattttga
      661 agcctgacta ttacgacttc ataaatgctt ttttagtttt aaacacttca atctaaaaga
      721 ataacccaag attggataaa ttattaatga ctcgccattc gtttcttagt gattctggat
      781 tatcaaatgg acttcctaat ctaatcagct agttcttccc ataaaatgcc atacttgact
      841 gaatcataat attgattatt taaaaatcta acttttctta tcactacttc ctttgtcatt
      901 ccacatttct caccattttt ttgcattcct ttgtttccag accatgtagt gaatctaata
      961 ggtggtagat aggcaaaata ctcaaataat tcttttaacc ataaaggaag agaaaggctc
     1021 ctcctgcaag agtcagtata atttttgctt actgctctct taggttttac tcactaaaac
     1081 tgttccatat tctttgatat aagaaaatgc atgcggtata aatttcttgc tcagctcaga
     1141 aaagatttgt cgtttaagaa aggctatttc ctccattgtc aatgaaggat ccaatgttgg
     1201 taattcaatc tcaaaattgt ttttttcgac aataggtaat tctaatgtga atgattcttc
     1261 ttcctttata gtattcgtca caagtagaga ctgatggaaa atactaggcc ggcgattttt
     1321 atatttattc ttacgtatcc tgtcaacgat aacttctatt tgatcaaaaa agtacgattc
     1381 atttacgtct ccaataagtt cggctaattc tttgaatgcc ttcgttacag gaagctgatt
     1441 cttctgtaca aaaaagtaat tagccaaagt aagcgggaag tgttctatag aggaaagtgt
     1501 gataaaacgg tacttctcat ttcctgcagt tgtcctcagc aattctgtct ctcggctgtt
     1561 tcttactcca taaaaatacg cttctttccg ttcatcttcg aaacaatacg cccaaataac
     1621 ttctcccatc gctaattttt gttcatcaac agagagtctc ttcaattgtt cagctatggt
     1681 ttcagcagcc gaagtggctt gttgcaagta atctttagga tcaaatattg gaaagttcaa
     1741 atgatatctt cgttcttccc tcttgattaa tcctgcatcg atcaataaat caagaaaatg
     1801 ggaaaattta ttttgcgaaa attctttttt caattctctt agaatcgttt cttgttctcg
     1861 atgttcatat agataatcgg tcaatgaaga aaaaagcgga tcatcggtca gtttttgata
     1921 aaagttttcc ttattttttg aataatagtt catatatgtt ctcttttcta tccgttttat
     1981 tttacttaaa aggcttttac gtaaaaagga ggaagaacaa gagtcataat tgactgttat
     2041 tctcctccat ttcttcggcc tttagtatgt tctatgtttc tttttatatc aatcgcttat
     2101 ccattcattt tgaatgcttc atactaccat ttgattagct aaatacagcc gtcaattgag
     2161 gtacgacttg ttttttacgt gaaacgacac ctttaagcaa agcacggttg ttggtcaaag
     2221 ttgtttggaa agcttcttct actttttctt ttcctttacc agcaacaagt aattctgaat
     2281 cactgtctaa gatgtttgtg acaactaata cgaacagatc gtagccattt gctttcattt
     2341 catcttccat tgctgcttct agctctgctt gtcgatccat cacttcatta aggtccactg
     2401 tattgacttg agcgatacga acgttggaac tgcccattgg gaaacttttt gcatccaaat
     2461 caagaagaac cgcagctgat ttatcactta gatttgttcc tgctttcaac atttcaagtc
     2521 cataagtgtt tagatcgact tcagcgattg ctgcaagttc ttttgcagct gcttcatctt
     2581 caggcgtaca tgttggtgat ttgaacaaca atgtatctga aatgatagct gagagcatga
     2641 ttcctgcgat ttctttagga attgttactt ggttttcttt atatagtttt aaaataatcg
     2701 tacttgtaca tccaactggt tctgcacgat agtacaatgg attagctgtt tcaaagtttg
     2761 caatacgatg gtggtctact acagctaaaa tatttacttc gctgatatct gaaatacttt
     2821 gttggaattc attatgatcc acaagcatga catctgcatt ttctgcttta gctgactcta
     2881 ctactcgtgg tgcagatagc tgaaagtggt ctaatgcgta ttgtgtttct tcattaggtg
     2941 tacctagagc aactgcttct gtttctttac ctaattcatt ttgcaagtgt gaaaacgcaa
     3001 tggctgcccc gattgcatct gtatctggat tttggtgacc aaaaactaaa atttttgaca
     3061 tgtcattcgc tcctttgtga tttctcctat ttaataatag caaaaaaacg gaaagaagca
     3121 atacttatct tctttccgat gcgacctatc gcgttaaata ttttgtatac tcttctgtat
     3181 gaagcaatcg tttagcattt tcgacacgtt cttttgtagg tggttcgatc ccttctaatg
     3241 gatatttcaa accaagtgtt tcccacttgt attttcccat cgtatgataa ggaagaatct
     3301 ctactctgtc tacatttgac aaggtttgaa taaagttgtc tagtcggatc aaatattcat
     3361 cataatcact tcgctcagga actagtacat ggcgaatcca aaccggtttg ttgatttttg
     3421 acaaatattg agccatttct aaaatatttt cattgtccca atgcgttagt tttttatgct
     3481 cttcattatc gatgtgtttg atgtcaaata acagtaaatc tgtatacttc atcaattctt
     3541 gaaaacgtga aaagaaaggt tcttcatacg taaaaggttt tccgcatgta tccaatgtag
     3601 tatgcatctt ccgctctttt gcttttttaa atagttcgat caaaaaatcg atctgcaata
     3661 gaggttctcc accactaaca gtaataccac ctttatctcc ccagtaaggt ctgaagcgtt
     3721 ctgctttatc taaaagttca tctgctgtgt attctttccc tccgccaata ttccaagtat
     3781 ctggattgtg acaaaattgg caacgcatgc ggcatccttg cataaacacg acaaagcgca
     3841 gtccaggtcc gtctactgaa ccgaaagttt cgatagaatg aacatagccg attgtttttt
     3901 cttccattta agttcacatc ttttctttga tttcatttta agtacaagtt agctgtcgaa
     3961 gcgaagcttt ctgaacaaaa tttttcgcaa ttgccgcttc gttatgtgta aaatttacag
     4021 ggacggaaca tacaagtcct gcccctgtaa atcgtttctt tcaaataagc atactgatta
     4081 ttcgattttg atgcgatggc tattcaacgt cctttcgacg ctaagtcgaa aaactttttt
     4141 cacgaacttt ttacattctg tcgtgtgaag ttctcatcaa gacatctaat tgttgttctc
     4201 ttgtcaagtc acggaattta actgcataac cagatacccg gatcgtcaag ttcggatatt
     4261 tcattggatt ttccattgca tcacgcaata aatcattact gaatacattg acattcaaat
     4321 gataagctcc ttttgagaag tatccatcta agacattacg caagttttcg attcttgtat
     4381 cttgttcttt tccaagacct gaaggattga ttgtttgggt atttgaaatt ccatccaatg
     4441 cacagccgta attcatttta gctgttgagt tcaggctagc taataaccca ctttgttccg
     4501 cattgtaact tggatttgct cctggcgcaa gtggttttcc agcttcacgg ccatcaggag
     4561 tatttcctgt atttttaccg taaacaacat ttgaagtaat cgtcaataat gaagttgttg
     4621 gttttgagtt gcggtaagtt ttatgacgtt tgatttgtcc catgaagtat tccaagatcc
     4681 atgaaactaa ttcatctgca cggtcatcat catttccgta tttagggaag tcgccatcca
     4741 cttcaaaatc aactgctatt cctttttcat tacggattgc ttttactttt gcatatttga
     4801 tcgctgacaa gctgtctgcc gcatgagaga tcccagcaat acctgtagca aatgtgcgtt
     4861 gcaagtcaga atccatcaaa gccagctgag ctgcttcata agaatattta tcatgcatat
     4921 aatgaatgat gttcaacgta ttgacatata gttctgctaa ccaatccatc atattcatga
     4981 agttatgttt cacttcttca aagtctagta tttctgatgt gattggacga tattctggtc
     5041 caacttgggc gccagtcact tcatcgactc cgccattgat ggcatataat agtgttttag
     5101 ccaagttagc acgagcgcca aagaattgca tgtcttttcc gacaactgtt cctgatacac
     5161 agcaagcaat cgcacaatca tcagaacccc attgttcacg aagctgatta tcattttcga
     5221 attgaatcga agaactttcg atagcgattc tgctagcata tgttctgaat ccttctggaa
     5281 ggtgttcaga atacaaaaca gtcaagtttg gttctggtga aggtcccata tttgttaatg
     5341 tatgaagcag acggaaatca ttctttgtaa ctaatgaacg accatctaat cccatacctg
     5401 caatggataa agtagcccag atagggaatc ctgagaataa ttggttatat tcaggtgtac
     5461 gcgcgaattt gaccatacgt aatttcatta ctaagtggtc gatcaattct tgtgcttctt
     5521 tttctgtgat tactccatta tccaaatcac gttggatata gatatcaagg aatgctgaga
     5581 tacgtccgat tgacatcgca gcaccatttt gtgatttgat tgctgctaaa tatccgaagt
     5641 atagccattg gacagcttct tttgcatttg cagctggttt agaaatatca tagccatatt
     5701 tcgcagccat ttctttcatt tgcgctaatg cacgatattg ttcagtgatt tcttcacgta
     5761 aacgaatcac ttcttcactc attgtatgat aaccagtcat atcatgatct tttttctttt
     5821 gttccatcaa atagtcgatt ccatacaatg ccacacgacg atagtcaccg atgatacgtc
     5881 cgcgaccgta agcgtctgga agccctgtaa tgattttgtt tttacgagca gcacgcattt
     5941 cagcagtata agcgtcaaat acaccttgat tatgtgtttt gcgccagtca gtgaaaatat
     6001 gtgtcaattc atcgtttggt gtatagccat tgctttttag cgcattgtta gccatattga
     6061 ttccgccgaa cggcatgaat gcttgtttca aaggtttgtc tgtctgtaga ccgacgattt
     6121 tttccaaatc tttatccaaa tagcctgcat cgtgtgaagt aatagttgat acgatttctg
     6181 tgtccatatc atagacaccg ttacgctcga attggatatc atttaacttt tgcaattcat
     6241 cccataattt ttctgttgct tctgttgggc cttccaaaaa gctgtcatca ccgcgatatt
     6301 cagtgtagtt attttgaata aagtctctag tgtctacgcc ttctttccat ttggtacctt
     6361 taaatccatt ccattgctcc attgtatgga cctcctaaat ttctgtttta tgtaacagtt
     6421 tttacgtgat tagtataaca cagcacccta tctttgcaag ggttttcttg ttttttattt
     6481 aacaattttt atgttttatt tacatatatt taaatcaacg ttgacatacc aacatttaag
     6541 cattgtgaac actttcatct ttttttgcaa acgtataagg atttattaaa aatgaatgaa
     6601 aaagagacgc cacctgtatt aatacagatg gcgtctcttc attaaaatac tacaacagtt
     6661 acgactcgat gaaaatctca ggagcatcga taacttcgat gacttctcca ccttttttct
     6721 catccatcgc aaatgatcca ttcgatgttc gatcacctat agggaaatca gttgggtcaa
     6781 tcgttaactg ctgccctttt tgattcatca aggttatttc ttttgatctt ccttccgaca
     6841 tatagataat acggtgcgga ttttttttca gctcacgcat gaccgtcact ccccgcttcg
     6901 cacgtgaagt ttggctgatt tcttgagcaa gcatgcgttt aacagctcct cgctgagtaa
     6961 cgattacgat cggcgtatca ccttctgcta acacaagcag cccgttgata atcctatctc
     7021 catctttcaa attcatagac ttcactccag ctgcctttgc tccaacaacg ggaacatctg
     7081 ttaaaggata gcgcaaaccc attccttgat gcgttactaa gaaaacatct aagcctggct
     7141 gatcttcttt cagatacaca gcaatcagtt catcttcttc cgcctttagc ttcatacagc
     7201 tgagcggacg gctcttatag gtgcgccatg gttcgaactc tgtcattcgt gtttgcttga
     7261 tcaatccatt cttgctgatg aatacaaatg ttttctctgc ttgaattttt tgatatggga
     7321 aaacagctaa aatcgattcg tctacagaca gattggagat tgtttgagaa atgtgctcac
     7381 cagcatcttt ccatttcaaa tcgggtagtt catggacagg gcggtagatc acatttgctt
     7441 tatttgtgat cagaagaaca tggtccaatg tactcaattc acctgagtat agcaagtagt
     7501 cgccttctcg catcccgatt tcttctggtt tggaagcact ataagaacga aggcttgttc
     7561 gcttcacata gccttctcgc gtcacactga caattacgtc ttcttgtgcc actaagactt
     7621 gcgtatcaat tttgatttct tcgatttcgt cttcgattgt cgttagacga gcggaagaat
     7681 aatttttttt cacttctcgc aattcttttt tcataacaga gaaaagctct ttgtcatttg
     7741 aaaggatttt attcaactct gtgatttgcg caatcaagct ttcggattcc ttttgaagct
     7801 ctgtgatatc cgtgttcgtc aaacggtata attgcaatgt gacaattgct tctgcttgtt
     7861 cttctgtaaa ttgaaagacg tcgactaaat tctttttggc gtcttttttg tctttacttt
     7921 cacgaatcgt tgcgattact tcatccagaa tcgataaagc tttcatcaat ccttctacga
     7981 tatgctgtct tttttgagct ttggctaaat cgaattgact acgttttgtt atgacttgct
     8041 ttcgatgctc gatataactt cttaaaatgt ctttcagtcc aacttgatgc ggagtcatat
     8101 ggtcgatagc gaccatattg aaattgtaat tgatctgtag ctcagtgttt ttgaacaaat
     8161 aattcaaaat tccttgagca ttggcgtctt ttttcaattc gacgacaatt tgcagacctg
     8221 ttctatcact ttcatctcgt acttcagcta ttccatcgat tttcttattc aaacggatct
     8281 catccatttt tttcaccaag gtcgctttat ttacttcata tgggatttct gtgatcacga
     8341 tttgctgttt attacccttc attgcctcta tctttgtttt cgaacgaaga ataacttttc
     8401 ctcgtcctgt ttcataagct tttttgattt catcttttcc ttgcaagatc cctcctgttg
     8461 gaaagtcagg tccaggaata tattccatca atttttcaag gctcgcttgt ggatgatcga
     8521 tcatatagat tgtgccatcg atcacttcag ctaagttatg agtcgggatt tctgttgcat
     8581 atccagcaga tatacctgtc gatccattga cgagaaggtt tgggtatcga gctggaagga
     8641 ctgttggttc tttttctgta tcatcaaaat tccacacgag atcgactgtt tctttctcga
     8701 tatctgctag catctctcca cttaacttgg ataatctggc ttcagtataa cgcatcgcag
     8761 caggtggatc accgtccata ctcccgttat tcccgtgcat ttcgatcagt acttcccgca
     8821 acttccagtc ttggctcatg cggaccatcg cttcataaat actgatgtca ccatgaggat
     8881 gataattccc catgatattt ccgacagatt ttgctgattt acggaatcct ttgtcatacg
     8941 tattgccatc tttgttcatg gcaaataata tccggcgttg cactggcttc aatccatcac
     9001 ggatatcagg cagtgcacgt tcttggataa tatatttgga ataacgtccg aagcggtcac
     9061 ccatcacttc ttctaaagtt aattcttgta cttcttgtcg attttccatc aggtgacctc
     9121 ctattctact tcaaataact gattgttttc atttttatct gctcgttctt catctaataa
     9181 atcattagaa actgaaggag agatttcagt atcctctttt ttatctaaga tactgccatc
     9241 ttcttcgagc gtaaattgca cgtgattttc gatccatttt cttcgaggtt ctactttgtc
     9301 acccatcagt gtggttactc gacgttcagc ttgcgcagca tcatcgatac ggacgcggat
     9361 caacgtacgg gaagtcggat ccatcgttgt ttcccacaat tgttccgcat tcatctctcc
     9421 aagtccttta tatcgctgca gcatatatcc tttgccgact tttttaatca ttgctgccaa
     9481 ttcgtcatct gtccacgcat attcaatgac ttgcttcttt ccttgtcctt tggatacttt
     9541 atataaaggc ggtaacgcga tatatacttt cccagcttca atcagcggtt tcatataacg
     9601 ataaaagaaa gtaagcagta atacttgaat atgggcgccg tctgtatccg catcggtcat
     9661 gatgattact ttgtcgtaat tacagtcttc gatagaaaac tcaggaccga ctcctgcacc
     9721 aatcgtatag atcatcgtat tgatttcttc atttttcaag atatcttgca ttttagcttt
     9781 ttcggtattg atgacttttc ctcgtaaagg tagaattgct tggaactttc tgtctcttcc
     9841 ttgtttagca gatccgccag cagaatcccc ttcgactaaa tacaattcat ttttcttagg
     9901 gtttcgggat tgtgcgggtg tcaattttcc tgaaagaaga gattcgcctt ttttacgctt
     9961 cttcccattt cggctttctt ctctggcttt acgtgctgct tctctcgctt ctctggcttt
    10021 gattgcttta cggaccaaca tctggctcat ttcgctattt tcctgcaaat aaaagcccat
    10081 ttgttcactg atgacgttat ctactacggt acgagcaact ggcgtaccaa gtttttcttt
    10141 tgtttgccct tcaaattgca gaagattttc aggtacacgg atagaaagta cagcagctaa
    10201 cccttctctg aaatcactac cttccagatt cttgtcccgt tctttcagca aaccaacttt
    10261 tctcgcatat tcattataag atttcgtcat cgccgctttc ataccagctt catgggttcc
    10321 cccatctttt gtccgaacgt tattgacaaa agataaaaca ttttctgaat aaccgtcatt
    10381 gtattgataa gcgacctcta cttcgatacc ttctttttct cctgaaaaat aaacaactgg
    10441 tgtcaatgta tctttttctt cattcagata atcaacaaat tctttgattc cttcttcgta
    10501 atgaaaaatt tccttgactg gttcttctcc acgaagatca gaaagagaga ttttaacgcc
    10561 ttttagaaga aaggcagatt ctcttaaccg ttcggctaaa atttcatatg aaaatttcgt
    10621 tgtactgaat attgtatcat ctggtaaaaa atggacagtc gtcccgtttg ctttttttgt
    10681 ttttcctatc tttttcaaag taccgtcagg tttaccgccg tttttaaatt tctgctgata
    10741 ctctacgcca tctctaacga tcgttacaat tagccatttc gataaggcat ttaccacact
    10801 ggctcctaca ccgtgcagtc caccagaagt tttgtaccct ccttgaccaa actttccgcc
    10861 agcatgcagc acagtaaaaa tcacttcaac tgttggtatt ccggaagcat gcattcctac
    10921 aggcatccca cgtccgctat ctgcaacagt tatactattg tcttcatgga tggtcacatc
    10981 aatctcactg ccataacctg ataatgcttc gtcaactgca ttatcgacga tttcatagac
    11041 taaatgatgc aagcctcggc tgtcagtgga tccaatgtac atccctggtc tttttctaac
    11101 tgcctctaat ccttccaata cttggatgga cgagtcattg tattcgttgt ttactttttt
    11161 tgccaaggca aacgctcctt caataatact ttgcggcaaa accgcaccac gattatcata
    11221 ctagaaaagg tcgtgaaaaa aaagagggta gacgagaaat caattttaat tatctcctaa
    11281 ccgctttttc gacattcccc tctatcgatc atctcttctt tgtggtcatt tttatttttg
    11341 gttttctttc aacttaccaa gttcaatctt aatacaacga tccattacga tatctgtacg
    11401 tcctgctttt cttagcatct cagctgcttc tttgttttca atccctaatt gtgcccagaa
    11461 cactttagca tcagttttca aaaaatcacc tgctatttca ggtaaaaatt cactccgtct
    11521 aaatacatct acgatatcga ttggatcatc gatttctgtc aaattcgcat agactttctc
    11581 gcctagaatt tcttcaccag ccaataatgg atttactggt atgattttat atccattgtc
    11641 ttgcaatacc tgtgcaacca tgtaacttgt tcgttctggt ttattgctca agcctacgac
    11701 ggcaatattt tttgcttctt ccaaatagtt gaaaatcgtt tcttgtgttg gattttttac
    11761 agacatttct gccacccctt gcttttcttt ttattccatc tattatactt gtatctttca
    11821 ttttttcaat tgttcctgtt acctattcat gctaaaataa aggatagttt tcaacttgaa
    11881 agagggtaaa aaatgaaatt gatcttatta cttattttag catatctttt aggttctatt
    11941 ccttccggtg tctggattgg caaattgttt ttcaaaaaag atatccgaca acacgggagc
    12001 ggtaatactg gaacgaccaa tacatttcgt gtattaggca aaacagccgg gattgttgtc
    12061 ctgcttatgg atattttaaa aggtacattg gctacttgtt tgccgatgat cttccacctt
    12121 tctgggatta accctctttg gtttggggta tgcgccattc ttggccatac atttccgatt
    12181 tttgctgggt tcaaaggagg aaaagctgtt gcgacaagtg caggaatgct tttaggattt
    12241 aatcctgtat tttttgttta ttcttgtatc atctttatcg ttagtttatt ttgtacaagt
    12301 atggtgagtt tgagcagtat gatcagtgca gtattgatca ctttatctac gatcgtcctt
    12361 ccttatgtag ctcctgtgat tttggctaag cccaattggt tactgacgat cattgccttt
    12421 ttagtttctt cctttatctt ttatcggcat aaagacaata tcaagcgtat cagaaaccat
    12481 actgaaagtc gaatcccgtt tggtttgaat agcagcaaga aaaaatcctg atactttttc
    12541 attccaaaga gataaaacgg gagtgtgaca aatcgatgtc acactccctt attacttaca
    12601 ggatagcttt tttacacacc ttggtaaacg gtgttccctg ctaacttctc ggtcacaacc
    12661 tcttacttca ctgtgatgga atattttgtc ttgaagattt catgggcacc tagttgattg
    12721 atccctactt tttccatcaa gtttccacta ctgttcaccg tgtcagcgat tccgcaccac
    12781 ggttcgatac aaacgaaagg cgcttctttt ggataaggag accagatccc tacataaggc
    12841 atatctttat agcttaaagt tacgctatgg gaagattttt cacttcgaat ggcgtacgca
    12901 tttagacccc ttgtttcaaa aaccaaggca tcttgctcaa aaagttcacg attcaaagcc
    12961 agattcgtat ttgtctgacc taacgtcttt tgatccatat cgatgaatgg gcctttcaat
    13021 ggtaggcgta atcttgattt caatggagaa aatgccagat agaaatcttc aaatgacaat
    13081 ccttcttcaa gaggtacatt gaaagcagga tgtccaccga tagaaaagaa catttctttt
    13141 tcaccggtat ttgccacttg ataatgaacc gtgatgccgt ctcctcctaa ttcatagctg
    13201 atcactaatt caaaatcaaa tggatacttt tctaatgtct ccggcgtact ccttaagatg
    13261 aaactagcca tttcttgtcc atgttcaatg acttcaaaat ccatatcccg agcaaatccg
    13321 tgttgcccca tttgatatgt tttcccttga taaacatact gatcatcttt taaacgtcca
    13381 acaaaaggga ataaaaccgg tgcatgtctg ccccaatacg ctggatcacc ttgccagata
    13441 tactctaaac catgttcttt tgatttcaga ctgatcaatt ccgcaccgtg ttcagcgatt
    13501 tcggcgatca gctggtcatt ttgaataata acagtcattg ccattcctcc aacttcgact
    13561 tactttttaa aatgggaagg ctggaacaaa catttctatc tgccccagtc cccttacttc
    13621 taataactgt gttttgctaa ctcgtcttta aaacgggaat tcaatacttt taaataagtt
    13681 cccttcatgc ctaacgaacg agattcaatg attccagctg attccaattt tcgtaaagca
    13741 ttgacgatca cagaacgagt gatgccgata ctatcggcaa tattagaagc agtcagacga
    13801 ccttcatctc catccaatgc ttcaaagata gcttgtacag ccttcaattc gctataagat
    13861 aatgtgttga ttgccatttg gacagccgtt gcactccgta catctgcttc gatgcttcga
    13921 gacttctgat aaaggatctg cataccgaca accgtagctc catattctgc taagactaga
    13981 tcgtcatctg caaacgattg ttccatacga gataagataa tcgtacccaa acgttcgcca
    14041 gctccaaaga tcggaacgac agtcgtcaga ccaaatggat atttctctct tgactctacc
    14101 ggaaatgcag taatatcact agtaattggg atattagctt cagttttcat caactgtgcc
    14161 actgtatcag tataagattc aggaaattgt ttctcaacaa acattgtttt gattcgttca
    14221 ttgttcacat cgtgacgttc attataacct agtaatactc cgtcacttcc tatgatatag
    14281 gtgttgctgt caagaatacc acctagtgtg acagccattt tatcgtaagg caaatctgct
    14341 tgcatgtcaa aagtattctt ttgctgcaaa agtgtgttta tcttgcgggt tttcgttagc
    14401 aagtctgtca ttgtcgtcct ccgatttttt ataaaatata gcgacttaaa tcttcatttg
    14461 ccgcaattga gccaagtttg tcatccacat atgcttctgt gatcgttatt tctcccattt
    14521 gcatatctgg tgcttcaaat aatagatctt ctagtagttt ttccaaaatg gtatgcagtc
    14581 tgcgtgcgcc gatattatct gtgtcacggt tgacatcgaa agcgatttga gcgatccgtt
    14641 caattgcttc ttttgtaaac gtaactgaca cattttctgt accgatcaac gcgacgtatt
    14701 gttttactaa agcattattt ggttcggtca gaatcttcac aaaatcttca gctgtcaaat
    14761 catctaattc tacccgtatc gggaagcgtc cttgaagttc tggaattaga tcacttggtt
    14821 tagacaaatg gaaagctcct gaagcaatga ataggacgtg atctgtttgg atgatcccgt
    14881 attttgtgtt tacttgtgaa ccttcgacaa ttggcaatat gtctctttgg acgccttctc
    14941 tggatacttc tccagattgc tgtgatttag aagtgatctt atcgatttca tcgataaaaa
    15001 taattccact ttcttccgct aatttgattg cctcactatg gatatcagca tccttgacta
    15061 attttgctga ttcttccttg atcaataact ctaaagcttc tttgactgtt accgtccgtt
    15121 ccactttttt cttcggtgtc aaagcaccta gagtttcatt taggtcgatc cccatttgct
    15181 ccaatccatt gttcatcatt ggtgcaggtt tcttttggtc ttcaacttca atggttactt
    15241 cacgactatc cagtaaaccc ttttgtagtt gttccaagac tgtacggcga ttgattcgaa
    15301 tatcttctgt cacttcttct tgcggttcag cggtattttg ctgactagca ttaaaaagat
    15361 tcatcatttg ttcaaattga ttagtggatt gtttctgttc ttttttgata ccaggtacca
    15421 agatttttac caaacgtttt tctgcatttt ttttagcact tgtgtataca cggctgtact
    15481 gctctttttc tacgatttgg atcgcatttt ctactaaatc ccgaaccatt gattctacat
    15541 ctcttccgac ataaccgact tcagtaaatt ttgttgcttc gactttgaca aatggtgcat
    15601 tgacgatttt tgccaaacgt cgtgcgattt ctgttttacc gacccctgta ggtccgatca
    15661 tcagcatatt ttttggtgta atatcttgtt gcattttttc atctaactgc aaacggcgat
    15721 agcggttacg caaagcaacg gcaacagatt tttttgctgc gtgctgtcct acgatatatt
    15781 catctaattc acttacgatt tgtcttggtg tttgttttaa ttcatacata tctactcacc
    15841 tcttacagtt cttctacgat aatattatga ttagtaaaga tacagatatc tgcggcaata
    15901 tttaacgcat tttctgcaat ttctttcgca ctcatgtcgt tttgagcata gtttttcatt
    15961 gctcgggctg cagccaacgc aaaattccct cctgatccga tagctaagat accatcatct
    16021 ggtgtgataa cttctcctgt acctgaaaca agaagcattt ctttatcatt cattacgatc
    16081 agcatcgctt caagtttttg catagattgc tgtgtccgcc attcttgagc taattcgaca
    16141 gctgcgcgtt gtagattccc gttatattcg tttagtttgc cttcaaattt ttcttctaaa
    16201 gtaaacgcat ctgcaacact tccggcgaaa cctacaacaa cttctccatt gtagatacgt
    16261 cgtactttct ttgcagtgcc tttcatgaca acagattcac ccattgttac ttggccatct
    16321 ccagccatag caaatttccc gtctttttcg acggcacaga tagtggttga atgaaatggt
    16381 gattcgacca tgtttgtttc ctcctaaatt tgttcttttc cttattttta cgctcttgga
    16441 tggaaagaac gatagttttt ttgtaaactt tcttttgtta catgggcata gatctgggta
    16501 gtcgataaat tggcatgtcc tagcaattct tggactgtcc gcaaatctgc cccattattt
    16561 aataaatgag tagcaaatgt atggcgcagc atgtgaggat ggatatcact atccaaactg
    16621 ctttttttga tcatttgatt caagacatat tcaattccag taggcgtgat tggttttcca
    16681 tgatgattca caaaaacgat atcatgctct tcgtgatatt ttgtcatcaa tacctggcgg
    16741 ccttccctga aatattcttg cagagcatct tgagcaaaag aacctagagg agcatatctc
    16801 tctttgttcc ctttaccatg gatcaacatg acctctgaat caaaatcaat gtctgatacc
    16861 gacaaactgc tacactcact taaacggatg cctgacccgt aaaggacttc cagtaaagcg
    16921 cggttcctca aatccaaagg actatctcct gctacgctgt caaaaagtgc ttgcatttca
    16981 ttttcataaa aaaaacgtgg cagtctcaaa tttttctttt tgagatgaac gtaagaaaat
    17041 ggattttctt ttatcacttc ttctttgaga agatactgat aaaacgaacg taggctggct
    17101 atcttgcgac tgatagaatt tctgctatag ctaggacgat gatcagctaa tgcactcaaa
    17161 tagacacgaa ccgtaaaata ttccgccttc agcagatcag cgtctccaga ttcctccaaa
    17221 aaatgtttga aatggagaat gtcttcttca tatgcttctt tcgttttttc tgaatatcct
    17281 ctttcgacaa tcaaataacg caaaaatttt tgttcccacg tggcttctgt cattttctca
    17341 ccttcctttt gtcccagaga ataatttagc acagcgttta taaaaagaca aataaattgt
    17401 cagaatttaa ccaatatttc taagtttgtt cacaattggc gcaattcctc acattttgtt
    17461 tgagaaattc ttaagaaaaa cgcaagaaaa agagcgagac tatccgtctc accctcttca
    17521 tggagtttcc ttactcattt gggattgcag ttttttctaa ttctgttttt attgcctcat
    17581 tagcttttaa agctcgttgt gcaattgctt catatcttgc ttttttgtct cgaatgcgct
    17641 ctggcagttc aggaaacaga ccatagttgg cattcatcgg ttggaaatgt tttccttccg
    17701 catgagtaat gtagtaagcc atacttccaa tagctgtttc ttgtggaaac tcgactggtt
    17761 ccatttcttt tgctaagcgt gcggcgttga ttccagcaag tagtccgctc gctgcacttt
    17821 caacatagcc ttctacacca gtcatctgac ctgcaaagaa cagatcttcg cgtttcgttg
    17881 attggtaagt cggtttcagc aattctggcg aattcataaa gctgtttcgg tgcatcacac
    17941 catagcgaac gaattcagca ttttctaacc caggaatcat ccggaaaaca cgtttttgct
    18001 cgccccattt caagtgagtc tgaaagccca cgatgttgta taaagaagct gcagcattgt
    18061 cttgacgcag ctggataaca gcataaggac gtttgccagt tttaggatct tccaatccaa
    18121 ctggtttcat aggtccgaaa agcatcgttt tgaatcctcg ctgtgccatt acttcaattg
    18181 gcatacatcc ttcaaaatat ttttctttct caaaagattt aagttctgcc acttcggcat
    18241 tgattagtgc ctcatggaaa gctttgaatt cctcttctgt catcggacag ttcaagtaag
    18301 ctgcttctcc tttgtcatag cgggacttca aatatacttt atccatatcg atcgtagctt
    18361 tgtctacgat tggtgcagct gcatcataga aataaaaacc atctgaacca ttgaatgctt
    18421 tgatgctttc tgctaatgga tgagaagtca aaggtcctgt tgctactacg gtgattcctt
    18481 ccggtatttc agtaatttct tcgtttatca ctgttactaa tggatgctct ttgacttttc
    18541 gggtaattgt ttcagaaaaa gaatcgcggt caacagctaa ggcaccacca gctggcactg
    18601 ctgtttcatc tgccgaagtg attactaccg aattcaatcg gcgcatttct tctttcaata
    18661 accctactgc attagccaaa ctgtttcctc ttaatgaatt ggaacatact agttcagcaa
    18721 aattacttgt gtgatgcgcc tcagtcgatt tgactggacg catttcatat aaacggacag
    18781 ggactcctgc ttgtgcgatc tgccaagcag cttcacttcc ggcaagccct gctccaacaa
    18841 catttaccat tttcaatgaa agaacctctt ttctattatt tttggatgtc ttcttcgtaa
    18901 tctccattta cacagactac ttgtttaccg cctttgactt tcttctcgac cagatatccg
    18961 tcacattttg gacaatttct gcccactggt ttatcccaag aagtgaattc acattctggg
    19021 aaacgggaac aaccatagaa tagtctgttt ttcttagatt ttcgttcaat gacttgtcct
    19081 tcatgacata ctgggcaagt cacaccgatt tcttttacga ttggtttcgt attccgacaa
    19141 tcaggaaaat tactgcaagc ataaaacttc ccatatttcc ctaattttat caccatcgga
    19201 tgtccacaga catcacaatc gaatcctgct ggttcatctt tgatttgaat tttttcgatt
    19261 ttttcttccg cattcgtcag ttctttttca aacggtttat agaaacgatc aaccactttg
    19321 acccacgctt ccgtgccttc ttcaatttta tccaaatctc cttccatttc tgctgtgaaa
    19381 tggatatcga cgatttgagg gaagaattca cagattaatg aattgacaat ttctcctagt
    19441 tcagtcggct caaatctttt ttgtgtcaat tttacatagt agcgtctttg gatcgtatca
    19501 agtgttggtg cataagtgga aggtcgccct accccgtttt cttctagtgc acgaatcaac
    19561 gtagcctcac taaaacgtgc tggtggttga gtaaaatgtt gttttggttc aatatcaaca
    19621 gattggacta catcgccttc ttcaagttct ggaagaatat tttctttctc ttctttgccg
    19681 tcatctctac cttccacata cacttgcatg aatcctttga atttgatttt tgaaccgttc
    19741 gcaatgaaga tgacaccatt ttgttctaat gtcactttca ttgtatctaa aatagctggt
    19801 gtcatctgac tagccacaaa acgtgaccag atcaatgtat aaagtttcaa ttgatcttta
    19861 tctaaatatt gtttgagttc atctggtgta cgaagcacac tcgttggacg cacagcttca
    19921 tgggcatctt gtgcgccttg agcatttttg atctttcggt gatcatgtct agaaaattct
    19981 tttccatacg tatcttcaat aaatgctgcg gcctctgctt ttgctgtatc ggcaatccgg
    20041 gtagaatcag tacgcatgta tgtaatcaag ccgactgttc cttgtttacc caaagtgatt
    20101 ccttcatata attgctgtgc taccatcatg gtttttcttg ttcgaaagtt taacttacgg
    20161 gcagcttctt gttgcaggct acttgtagtg aaaggattcg cagggtttct tttgcgctct
    20221 ttcttttcta cttttttaac ttcgaagtct gttccatcaa ttctagcagt gacttctttt
    20281 acactttgtg catctggtaa tttctttttc ttaccgtcga ttccccagaa atttgctttg
    20341 aatttcttgc gagctttttt aaaattaccg tcgatgctcc aatattcttc tggaacaaat
    20401 tctcggatct ctttttcacg gtcaataata atttttaaag cgacagattg gacacgtcct
    20461 gcacttaacc ctttcttgac cttacgccag agaataggac tgatcgaata accaacgatt
    20521 ctgtctaagg tacgacgagc ttgctgtgca tctactaaat ctacatcgat cgttcttggt
    20581 tccttaaaag ctgctttgac tgcgtctttt gtgatttcat tgaagaccac ccgatttttt
    20641 tctttcagat ccaacccaag taggtacgct aaatgccaag caatcgcttc tccttctcta
    20701 tccgggtcac ttgccaagta aactttttga gctttttttg ctgcggcttt caggcttttg
    20761 atgacatcgc cttttccgcg tatagaaata taatggggtt cataattgtt ttcaaaatcg
    20821 atacccattt tacttttcgg taaatctctt atatggccaa cactggccat tactttatag
    20881 tttcgtccaa gatatttctc aattgtcttg gcttttgcag gtgattctac aatcactaaa
    20941 tatttatacg ccattactga atggactcct ttccattgtc gtgttcctac tattaaaaga
    21001 ggattcatac aaatcgtagc taatcatatc cattcagaaa acgtttgtca agaaaagcaa
    21061 atgaaaagta tggtgttatt ctttaaattc tgccaagata tcttcacccg aaaccgtgca
    21121 aactgcaccg tcttggatca aatgatgaca tccggtagaa tgttctgtta aaatatttcc
    21181 tggaacagaa aaaactgttc ttccaagatc caatgcttgc tgtgctgtta tcagagagcc
    21241 gctttttttc ttggcttcaa agacacatgt tcctttagac aaagctgcga taatccgatt
    21301 tctcattgga aaacaatgct ttttcgggcc ggattgagga ggatattcac tcaaaagaag
    21361 atgatcattt gcaatcacct cttgcaagtc acgattttca gcaggataga cttgctctat
    21421 ccctgttcca atgactgcga ttgtccgtcc accatgagag attgctgctt ggtgaccgta
    21481 tgtatctatc cctttcgcgc agccactgac gatcacatat ccagcttcta tcactggttt
    21541 tagtaaataa taaactgttt ttttggctaa agcagaagtg tgtcttccac caacaacgct
    21601 taacatgtta caagaaagta aagatagatc accattataa aacagtacaa ttggcggata
    21661 ggtcaagtga agcagctgag ggggatattt gggatcaaag caagtgataa acgactgttt
    21721 cgattggatt ttgccccagt tagcgtgaat ttcctgccaa cttttgcgaa acagctcccg
    21781 gtgtttggtt attttaccta tcttaatgat gtcttcaaat ccgatttctt tataaggata
    21841 ctgcatcatc cagttagcaa gtgcccattt tccttgacta ctgattcctt ttgtataagc
    21901 aagtttcaat aaattttctt ctatttgata catcataaaa ccgcctttcg tctctctttc
    21961 taataagata cgtaaaagcg gctgtttttt attgtaaata tttttttaca ggtgcaaaac
    22021 ttttacgatg gattggcgta attccttgtc gttcaagtcc ttctaagtgt tcctttgttc
    22081 cataacctgc attatgcgag aaaccatatc caggataaag cacgtcatat tctttcatca
    22141 attggtctcg atatacttta gcaatgatac ttgcagcagc aatcgacaca gatcgtgcat
    22201 cccctttgat tattttttcc tgctgaatag gcaaatccaa ggtcatcgca tcgatcaaca
    22261 aatagcttgg tacaatggac aatttttcta ctgcttggat catcgctaat ttacttgctt
    22321 cataaatatt cacatcatcg attgtctgct gatcaacgat cccgatcccg attgccaggg
    22381 ctttttcttg aacttgttga taaagaagtt ctcgcttttt ctctgataat tgtttggagt
    22441 cattcagccc taatatgatc tcatttttcg gcaagataac tgcagctgcc acgacaggtc
    22501 cagctaatgg tcctcttcct acttcatcaa tgccggcaat ccattggtga ccttgtgcgt
    22561 acccttgctt ttcaaagcaa aacatttctt catatttttc cttaattaat gcttcctttt
    22621 tcaatcgtcg ttcaaaagaa gccagtgctt gctgcacgcc tttgcgtgga tcatttctta
    22681 atgattctag tgtaggatca tctattctct gtatgctttt caatcgttct ttgatacttt
    22741 ggatggattc agtcatcact tttttgctcc atttcttcgc aacgatccag tgtgtatcgt
    22801 ccaagttttc cttgacgaat atcatgcacg actaattcgc tgccacgatc atagtcttct
    22861 ttatacccac gttttgctgt aatatccatc agtaattccg gtgccaattt ttcttcttgc
    22921 tcgattgata aaccataacg atcgctgatt cttcctgaat aatatcgggc aaaaaagtct
    22981 aatccgtaaa tcgctaaatc atctaaatgg acaagctggt ctttgatcgc gcctgtcagc
    23041 gctagttttt tgccgatttc ttcatcttca aatttgggcc ataaaatccc tggtgtgtct
    23101 aacaattcca actcattacc taaacgaagc cactgctgac cttttgttac gccaggctta
    23161 tttcctgtct gtgcaatttt cttgcctact agtcgattca tcaacgtaga tttcccaaca
    23221 tttggaatac ctaagaccat tgcccgaata gcgcgaggct tgatcccccg agcagcatct
    23281 cttgcccgtt tttctttcaa tgcttcttta gccgcaggta ctagaccttt aattccttta
    23341 ctttcttgtg ccgtgatagc taatgcaaga tggccttgtt tacgaaaata ttcttgccac
    23401 agctttgtct gttgtggatc tgccagatcc gctttattca ataaaacaag gcgaggtttt
    23461 tgctgaatga tctgatctag cattggatta cgagaagata aaggtaatcg tgcgtctacc
    23521 aattcaaata cgatgtccac atatttcatt ttttcagaaa cttctcgtcg ggcttttgcc
    23581 atatgtcccg gaaaccattg gatggtcatt gttctctcca ttcctttcat accggaatat
    23641 tccccggctt tacttgtttt tttctttttc aatataaatt cactttttta ttataaagaa
    23701 attccatcta ttagttaagt gaaagtgaac cagtcctcat tctatcacat cttcactttt
    23761 ttgatgatgc aaatttttat tttggtttct atatatgaat ctaaattttt gacagcgttt
    23821 cctaaataag agatgataga cgaacaaaac agaaaggaga cgaaacaaat gaatagtatg
    23881 gtttttgtta attttcctgt tgctgatgtc aatcgttccg ttgcttttta tgaaaagctg
    23941 ggttttaaaa aaaatgaaga attttctact gaagaagctg gttcaatggt atgggatgac
    24001 actttttgga taatgcttct ctctcatgat ttttataaaa aatttataaa agataaagaa
    24061 atagctgata ataaaaaaac aagtggtgct ttgatttcgt ttagtgttga aaatgcggat
    24121 gaagtaaaaa aatttgctca aattgcaaaa gaaaatggtg gtactttcca cgaagtagat
    24181 atgggaatgc ctgaagaaga aatgttttca ttagaagtcc aagatctaga cggaaataca
    24241 ttagaacccg tatggatgaa gatgtaagca gataactaat ctgacgtttt ttataacaaa
    24301 aaaacaaagg cggatgatac gataagtatt ctccgcctct atttttttta ttcgtacaat
    24361 ctctttttta cagtttataa aagcagtctt ctcacccttt ttttaattgt tagatgccag
    24421 tgtatgtaaa tcaccaatgt gtcatgacac attggtgatt ttatggtatc cttttattat
    24481 taactgacaa aggagagaat attttgaaca ctttgattgc aaaagacgtt tttagattgc
    24541 ccgaaatgaa tgcaagattg ttgtcgacct ctgctctttt attagccctt gatgtcatct
    24601 tatacaagat agcgattggt cctgcttatt ttcaaatcac attcggattt ttaagtatgg
    24661 cgcttatggg ttatctatat ggaccgatct gggctggttt gcttgaaatg ttgtcgaaca
    24721 ctttaaattt cacgatattt ggttcaggaa cgtttcaagc tgcttttctt atcacggctt
    24781 tccttggtgg atttattaat ggcttatttt tatatcataa aaagataaac ctgccttccg
    24841 tcatcgctgc ccaattattg attatgatcc cagtcagcct ttttatcaat tcttggctga
    24901 tgtctgtttt gtttggtgca gatttcaaag taatttttct aggtagattc atcaggaata
    24961 tgatccaaat ccctattcaa ataatggttg tttattcatt tatcaaaact ttagaggcta
    25021 gaaaagtgtt taaacgagct gtttgatttt acgtactaag gtagtaaact gattttggat
    25081 taaacaataa agcgtgcagt ctcaacagac tgcacgcttt attgtttctc actggatcca
    25141 ttttatttca tcaagtggat aatagacaaa tcttgcttta cctagaatat cagttttatt
    25201 gatcgtgcca aacgaacgac tatcttttga aactcttcga ttatctccaa gtacaaaata
    25261 attatcttcc ggaagcttgg attgtcccgt tagctcttgt agtgtaaagt ccgtcgtata
    25321 ggatgcattt gcatgatctg atttcaggtt tttagttaga taaggttcct taactacttt
    25381 cccattgatc ttcaattgat cattttggta gctgacattt tctcctggaa gaccgattac
    25441 tcgtttgata taaatcgtgc catctgctaa ttgaaaaaca acaatatcaa atcgtctgat
    25501 ctccgaaaat ttttccatca caaccatatc accttgcttc aacacgtttt ccattgaatt
    25561 gccttccact ggtaccggga tgaataaaaa tccccgcaac atgaacgcaa ggaatacaga
    25621 aacaagtaag tatttgaata caagccagaa gcgattaata tagcgttgtt tttttgtcaa
    25681 tctctatcaa tccatccctc tttttttcat tctttcttta ttataagaaa aatatcaaaa
    25741 aaa
//
LOCUS       DASJST010000026        25536 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJST010000026 DASJST010000000
VERSION     DASJST010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.026"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U4B05_000850"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U4B05_000850"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2050005.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="U4B05_000851"
     CDS             complement(937..1518)
                     /locus_tag="U4B05_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2050006.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U4B05_000852"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U4B05_000852"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN2050007.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="U4B05_000853"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="U4B05_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN2050008.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="U4B05_000854"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="U4B05_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN2050009.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="U4B05_000855"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="U4B05_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN2050010.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="U4B05_000856"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="U4B05_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2050011.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="U4B05_000857"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="U4B05_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN2050012.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="U4B05_000858"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="U4B05_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN2050013.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="U4B05_000859"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="U4B05_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN2050014.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="U4B05_000860"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="U4B05_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN2050015.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="U4B05_000861"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="U4B05_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN2050016.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="U4B05_000862"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="U4B05_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN2050017.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="U4B05_000863"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="U4B05_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN2050018.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="U4B05_000864"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="U4B05_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN2050019.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="U4B05_000865"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="U4B05_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN2050020.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="U4B05_000866"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="U4B05_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN2050021.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="U4B05_000867"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="U4B05_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN2050022.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="U4B05_000868"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="U4B05_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN2050023.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="U4B05_000869"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="U4B05_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN2050024.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="U4B05_000870"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="U4B05_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN2050025.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="U4B05_000871"
     CDS             13972..14157
                     /locus_tag="U4B05_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN2050026.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="U4B05_000872"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="U4B05_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN2050027.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="U4B05_000873"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="U4B05_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN2050028.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="U4B05_000874"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="U4B05_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN2050029.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="U4B05_000875"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="U4B05_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN2050030.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="U4B05_000876"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="U4B05_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN2050031.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="U4B05_000877"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="U4B05_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN2050032.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="U4B05_000878"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="U4B05_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN2050033.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="U4B05_000879"
     CDS             18232..18879
                     /locus_tag="U4B05_000879"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN2050034.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="U4B05_000880"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="U4B05_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN2050035.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="U4B05_000881"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="U4B05_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN2050036.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="U4B05_000882"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="U4B05_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN2050037.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="U4B05_000883"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="U4B05_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN2050038.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="U4B05_000884"
     CDS             20317..21255
                     /locus_tag="U4B05_000884"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN2050039.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="U4B05_000885"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="U4B05_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN2050040.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="U4B05_000886"
     CDS             21935..22774
                     /locus_tag="U4B05_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2050041.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="U4B05_000887"
     CDS             22750..23619
                     /locus_tag="U4B05_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2050042.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="U4B05_000888"
     CDS             23609..24406
                     /locus_tag="U4B05_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2050043.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="U4B05_000889"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="U4B05_000889"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2050044.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8299 a         4712 c         5497 g         7028 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctc
//
LOCUS       DASJST010000027        24975 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJST010000027 DASJST010000000
VERSION     DASJST010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.027"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(43..2187)
                     /locus_tag="U4B05_000890"
     CDS             complement(43..2187)
                     /locus_tag="U4B05_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2050045.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="U4B05_000891"
     CDS             complement(2544..3917)
                     /locus_tag="U4B05_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2050046.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="U4B05_000892"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="U4B05_000892"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN2050047.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="U4B05_000893"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="U4B05_000893"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN2050048.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="U4B05_000894"
     CDS             complement(5365..6378)
                     /locus_tag="U4B05_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2050049.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="U4B05_000895"
     CDS             complement(6459..7058)
                     /locus_tag="U4B05_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2050050.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="U4B05_000896"
     CDS             7244..7945
                     /locus_tag="U4B05_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050051.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="U4B05_000897"
     CDS             7955..10666
                     /locus_tag="U4B05_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050052.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="U4B05_000898"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="U4B05_000898"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN2050053.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="U4B05_000899"
     CDS             complement(12805..13986)
                     /locus_tag="U4B05_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2050054.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="U4B05_000900"
     CDS             14238..15761
                     /locus_tag="U4B05_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN2050055.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="U4B05_000901"
     CDS             complement(15830..16558)
                     /locus_tag="U4B05_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2050056.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="U4B05_000902"
     CDS             complement(16571..17851)
                     /locus_tag="U4B05_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2050057.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="U4B05_000903"
     CDS             complement(17992..18594)
                     /locus_tag="U4B05_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2050058.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="U4B05_000904"
     CDS             18839..19045
                     /locus_tag="U4B05_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050059.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="U4B05_000905"
     CDS             19357..20076
                     /locus_tag="U4B05_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2050060.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="U4B05_000906"
     CDS             complement(20157..20840)
                     /locus_tag="U4B05_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN2050061.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="U4B05_000907"
     CDS             21058..23244
                     /locus_tag="U4B05_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN2050062.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23590..24000
                     /gene="spx"
                     /locus_tag="U4B05_000908"
     CDS             23590..24000
                     /gene="spx"
                     /locus_tag="U4B05_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2050063.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            24014..24370
                     /locus_tag="U4B05_000909"
     CDS             24014..24370
                     /locus_tag="U4B05_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN2050064.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8367 a         4437 c         4872 g         7299 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga caagaatttt
    23401 gtcacacctc ccttatttat tttgaaaatg agaatactat tatcaaaata gagaatatat
    23461 agaaaaattc tcattattca aacgtaaata atcagttatt ttgatttttt ttatatttta
    23521 ttagctaata attggtattt taaaagaaat tactgtagaa ttggattata attaaaaagg
    23581 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
    23641 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
    23701 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
    23761 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
    23821 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
    23881 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
    23941 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
    24001 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
    24061 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
    24121 cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
    24181 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
    24241 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
    24301 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
    24361 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
    24421 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
    24481 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
    24541 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
    24601 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
    24661 taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac
    24721 ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt tgctgtaaga
    24781 tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt gacaattact tgtctctctg
    24841 atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc ctgatgaagt
    24901 agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat
    24961 tcaagacaac aaaca
//
LOCUS       DASJST010000028        24853 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJST010000028 DASJST010000000
VERSION     DASJST010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.028"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..1106)
                     /locus_tag="U4B05_000910"
     CDS             complement(324..1106)
                     /locus_tag="U4B05_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050065.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1171..2007)
                     /locus_tag="U4B05_000911"
     CDS             complement(1171..2007)
                     /locus_tag="U4B05_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2050066.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2025..3434)
                     /locus_tag="U4B05_000912"
     CDS             complement(2025..3434)
                     /locus_tag="U4B05_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2050067.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3448..5271)
                     /locus_tag="U4B05_000913"
     CDS             complement(3448..5271)
                     /locus_tag="U4B05_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2050068.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6114..6440)
                     /locus_tag="U4B05_000914"
     CDS             complement(6114..6440)
                     /locus_tag="U4B05_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050069.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6486..6848)
                     /locus_tag="U4B05_000915"
     CDS             complement(6486..6848)
                     /locus_tag="U4B05_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEN2050070.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6885..7099)
                     /locus_tag="U4B05_000916"
                     /pseudo
     CDS             complement(6885..7099)
                     /locus_tag="U4B05_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7227..7562
                     /locus_tag="U4B05_000917"
     CDS             7227..7562
                     /locus_tag="U4B05_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2050071.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7638..9635)
                     /locus_tag="U4B05_000918"
     CDS             complement(7638..9635)
                     /locus_tag="U4B05_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050072.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9733..10008)
                     /locus_tag="U4B05_000919"
     CDS             complement(9733..10008)
                     /locus_tag="U4B05_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050073.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10240..10974
                     /locus_tag="U4B05_000920"
     CDS             10240..10974
                     /locus_tag="U4B05_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2050074.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11013..12155
                     /locus_tag="U4B05_000921"
     CDS             11013..12155
                     /locus_tag="U4B05_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2050075.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12250..13641)
                     /gene="sufB"
                     /locus_tag="U4B05_000922"
     CDS             complement(12250..13641)
                     /gene="sufB"
                     /locus_tag="U4B05_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN2050076.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13646..14116)
                     /locus_tag="U4B05_000923"
     CDS             complement(13646..14116)
                     /locus_tag="U4B05_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN2050077.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14103..15338)
                     /locus_tag="U4B05_000924"
     CDS             complement(14103..15338)
                     /locus_tag="U4B05_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2050078.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15335..16621)
                     /gene="sufD"
                     /locus_tag="U4B05_000925"
     CDS             complement(15335..16621)
                     /gene="sufD"
                     /locus_tag="U4B05_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN2050079.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16634..17407)
                     /gene="sufC"
                     /locus_tag="U4B05_000926"
     CDS             complement(16634..17407)
                     /gene="sufC"
                     /locus_tag="U4B05_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN2050080.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17604..18452)
                     /locus_tag="U4B05_000927"
     CDS             complement(17604..18452)
                     /locus_tag="U4B05_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2050081.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18476..19162)
                     /locus_tag="U4B05_000928"
     CDS             complement(18476..19162)
                     /locus_tag="U4B05_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050082.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19155..20189)
                     /locus_tag="U4B05_000929"
     CDS             complement(19155..20189)
                     /locus_tag="U4B05_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2050083.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20252..20508)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20584..20922)
                     /locus_tag="U4B05_000930"
     CDS             complement(20584..20922)
                     /locus_tag="U4B05_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN2050084.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20924..21280)
                     /locus_tag="U4B05_000931"
     CDS             complement(20924..21280)
                     /locus_tag="U4B05_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN2050085.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21286..22473)
                     /locus_tag="U4B05_000932"
     CDS             complement(21286..22473)
                     /locus_tag="U4B05_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2050086.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22664..23509)
                     /locus_tag="U4B05_000933"
     CDS             complement(22664..23509)
                     /locus_tag="U4B05_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2050087.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23743..24708
                     /locus_tag="U4B05_000934"
     CDS             23743..24708
                     /locus_tag="U4B05_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN2050088.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7264 a         5228 c         4223 g         8138 t
ORIGIN      
        1 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
       61 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      121 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      181 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      241 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      301 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      361 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      421 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      481 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      541 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      601 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      661 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      721 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      781 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      841 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
      901 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
      961 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1021 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1081 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1141 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1201 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1261 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1321 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1381 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1441 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1501 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1561 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1621 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1681 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1741 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1801 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1861 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     1921 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     1981 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2041 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2101 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2161 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2221 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2281 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2341 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2401 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2461 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2521 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2581 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2641 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2701 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2761 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2821 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     2881 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     2941 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3001 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3061 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3121 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3181 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3241 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3301 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3361 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3421 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3481 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3541 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3601 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3661 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3721 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3781 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3841 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     3901 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     3961 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4021 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4081 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4141 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4201 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4261 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4321 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4381 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4441 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4501 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4561 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4621 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4681 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4741 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4801 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4861 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     4921 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     4981 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5041 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5101 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5161 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5221 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5281 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5341 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5401 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5461 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5521 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5581 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5641 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5701 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5761 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5821 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
     5881 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
     5941 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
     6001 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
     6061 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
     6121 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
     6181 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
     6241 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
     6301 tgtaaaatct gtgttaccaa tggtttcgat gacttctcta atccagatgt acctgtcatt
     6361 tttgaaaaaa tgatacctat aatagccaag acaacgataa ctataatgat aaacttcttg
     6421 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ctcttatccc
     6481 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
     6541 atcatcaaaa tcgtacccat ttgttcatct ttatctcgat ataagtagcc cataataaca
     6601 gcaacagcac caaatacaac aggaataaat gctaacgcta ctaccataga aatccaacca
     6661 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
     6721 attgtctgct cagtactttt ctttactgcg tctgctccag ttgtctcatc tgtttcttgt
     6781 agtgctccgc aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
     6841 caatacatgt cttctcctcc taaaaacatt caattgttta ttagttaact gtttacttta
     6901 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
     6961 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
     7021 acattcattt ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga
     7081 gattacttac ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt
     7141 atttcaactc ataaaatata aaaaaacttc aaataccatt acaaatacaa caacttgttg
     7201 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
     7261 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
     7321 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaaccg
     7381 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
     7441 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
     7501 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
     7561 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
     7621 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
     7681 ccatcatctc ttgttacact tgtaaattga taaagaggaa ctaattcaac tcctgataat
     7741 atcaattctt ttattacttc atctatccct gctccttttg ctaggtagac taaaaaacta
     7801 ctacttgctt tcacattgta tagtctctta atatccttag aagctcgttc aagagtgaat
     7861 ccttgtttaa caaagtagcc tctgttttct tttaaagcat tttccacgct gttattccca
     7921 acattacgta gacgttcaca aacagtgtta gtaagttctt tctctgtcat tagatatgtt
     7981 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
     8041 ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
     8101 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt ttccagaagt
     8161 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
     8221 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
     8281 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
     8341 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
     8401 aaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
     8461 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
     8521 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
     8581 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
     8641 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
     8701 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
     8761 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
     8821 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
     8881 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
     8941 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
     9001 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
     9061 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
     9121 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
     9181 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
     9241 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
     9301 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
     9361 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
     9421 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
     9481 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
     9541 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttctttatct
     9601 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
     9661 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
     9721 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
     9781 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
     9841 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
     9901 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
     9961 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    10021 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    10081 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    10141 acataatacc tcgataaaaa agaggtgtca atatctttgt aaaatttatt tacaacaccc
    10201 ctttttcgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    10261 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    10321 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    10381 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    10441 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    10501 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    10561 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    10621 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    10681 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    10741 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    10801 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    10861 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    10921 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    10981 gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga
    11041 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    11101 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    11161 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    11221 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    11281 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    11341 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    11401 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    11461 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    11521 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    11581 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    11641 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    11701 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    11761 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    11821 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    11881 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    11941 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    12001 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    12061 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    12121 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    12181 tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct
    12241 ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat tgagttcgac
    12301 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    12361 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    12421 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    12481 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    12541 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    12601 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    12661 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    12721 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    12781 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    12841 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    12901 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    12961 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    13021 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    13081 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    13141 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    13201 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    13261 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    13321 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    13381 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    13441 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    13501 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    13561 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    13621 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    13681 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    13741 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    13801 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    13861 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    13921 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    13981 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    14041 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    14101 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    14161 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    14221 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    14281 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    14341 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    14401 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    14461 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    14521 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    14581 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    14641 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    14701 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    14761 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    14821 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    14881 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    14941 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    15001 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    15061 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    15121 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    15181 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    15241 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    15301 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    15361 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    15421 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    15481 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    15541 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    15601 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    15661 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    15721 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    15781 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    15841 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    15901 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    15961 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    16021 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    16081 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    16141 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    16201 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    16261 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    16321 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    16381 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    16441 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    16501 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    16561 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    16621 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    16681 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    16741 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    16801 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    16861 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    16921 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    16981 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    17041 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    17101 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    17161 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    17221 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    17281 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    17341 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    17401 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    17461 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    17521 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    17581 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    17641 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    17701 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    17761 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    17821 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    17881 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    17941 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    18001 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    18061 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    18121 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    18181 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    18241 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    18301 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    18361 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    18421 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    18481 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    18541 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    18601 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    18661 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    18721 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    18781 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    18841 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    18901 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    18961 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    19021 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    19081 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    19141 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    19201 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    19261 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    19321 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    19381 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    19441 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    19501 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    19561 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    19621 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    19681 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    19741 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    19801 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    19861 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    19921 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    19981 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    20041 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    20101 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    20161 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta taatttttca
    20221 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    20281 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    20341 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    20401 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    20461 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    20521 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    20581 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    20641 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    20701 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    20761 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    20821 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    20881 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    20941 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    21001 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    21061 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    21121 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    21181 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    21241 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    21301 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    21361 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    21421 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    21481 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    21541 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    21601 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    21661 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    21721 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    21781 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    21841 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    21901 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    21961 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    22021 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    22081 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    22141 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    22201 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    22261 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    22321 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    22381 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    22441 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    22501 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    22561 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    22621 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    22681 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    22741 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    22801 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    22861 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    22921 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    22981 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    23041 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    23101 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    23161 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    23221 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    23281 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    23341 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    23401 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    23461 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    23521 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    23581 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    23641 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    23701 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    23761 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    23821 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    23881 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    23941 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    24001 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    24061 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    24121 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    24181 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    24241 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    24301 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    24361 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    24421 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    24481 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    24541 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    24601 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    24661 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    24721 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    24781 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    24841 caagaggtcg taa
//
LOCUS       DASJST010000029        24815 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJST010000029 DASJST010000000
VERSION     DASJST010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.029"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(501..1055)
                     /locus_tag="U4B05_000935"
     CDS             complement(501..1055)
                     /locus_tag="U4B05_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN2050089.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1181..3110)
                     /locus_tag="U4B05_000936"
                     /pseudo
     CDS             complement(1181..3110)
                     /locus_tag="U4B05_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3416..4132)
                     /locus_tag="U4B05_000937"
     CDS             complement(3416..4132)
                     /locus_tag="U4B05_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2050090.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4133..5956)
                     /gene="glpO"
                     /locus_tag="U4B05_000938"
     CDS             complement(4133..5956)
                     /gene="glpO"
                     /locus_tag="U4B05_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN2050091.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5970..7466)
                     /gene="glpK"
                     /locus_tag="U4B05_000939"
     CDS             complement(5970..7466)
                     /gene="glpK"
                     /locus_tag="U4B05_000939"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN2050092.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7602..9062)
                     /locus_tag="U4B05_000940"
     CDS             complement(7602..9062)
                     /locus_tag="U4B05_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050093.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9052..10347)
                     /locus_tag="U4B05_000941"
     CDS             complement(9052..10347)
                     /locus_tag="U4B05_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2050094.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10679..11356)
                     /locus_tag="U4B05_000942"
     CDS             complement(10679..11356)
                     /locus_tag="U4B05_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN2050095.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11386..12477)
                     /locus_tag="U4B05_000943"
     CDS             complement(11386..12477)
                     /locus_tag="U4B05_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN2050096.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12578..13012)
                     /locus_tag="U4B05_000944"
     CDS             complement(12578..13012)
                     /locus_tag="U4B05_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2050097.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13596..15053)
                     /gene="ascB"
                     /locus_tag="U4B05_000945"
     CDS             complement(13596..15053)
                     /gene="ascB"
                     /locus_tag="U4B05_000945"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2050098.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15050..16945)
                     /locus_tag="U4B05_000946"
     CDS             complement(15050..16945)
                     /locus_tag="U4B05_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2050099.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17003..18778)
                     /locus_tag="U4B05_000947"
     CDS             complement(17003..18778)
                     /locus_tag="U4B05_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2050100.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18828..20324)
                     /locus_tag="U4B05_000948"
     CDS             complement(18828..20324)
                     /locus_tag="U4B05_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2050101.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20518..22140)
                     /locus_tag="U4B05_000949"
     CDS             complement(20518..22140)
                     /locus_tag="U4B05_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2050102.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22468..23781)
                     /locus_tag="U4B05_000950"
     CDS             complement(22468..23781)
                     /locus_tag="U4B05_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2050103.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24026..24250)
                     /locus_tag="U4B05_000951"
     CDS             complement(24026..24250)
                     /locus_tag="U4B05_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050104.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24270..24692)
                     /locus_tag="U4B05_000952"
     CDS             complement(24270..24692)
                     /locus_tag="U4B05_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN2050105.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT         7135 a         5402 c         4067 g         8211 t
ORIGIN      
        1 tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt
       61 gagctaatac cgaaggtcag ctgattgaac accgtttaag gaagagattg tgaaaaagtc
      121 gcttagtttc gagtattaag aaaaattttg tgaaaatgga tttctacatt tttgcataaa
      181 tttggcttaa taccgagaaa ctggcttttg aataccgttt aaggaagagg ttgtaaaatc
      241 agcggtttga cctgagtatt agagaaacaa acggaaaaat agctgctcat attttgccgt
      301 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc
      361 taccatttaa ctccgaatca caagaaataa tttcaaaaac aacttctcct gtttttggtt
      421 cggttggctt attatcgaga agtagctact gggagtctat ttatcaaaaa aaggaatgcg
      481 acataggtca cacccctttt ttattttctt tgaaacatct ttcggtactc gacagcatac
      541 aagacaaaag attgtaaaag accgtaagtg atgacggcaa aaatgactct cacccacagc
      601 gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa catagatgac agcagtcgct
      661 actttgccat accacttcgc tccacttaat ttgatatttt tagatagccc gattaatcca
      721 gctacaaaca taaagccttc ttttatcaag aatagaccaa acaacagcca aagccacggt
      781 cgataccatg ccatagacaa aatcagaaaa agttgtgtca atttatctgc aaacggatca
      841 agcaccttcc ctaagtctgt tacctgatta tatctccgag caatgacccc gtctagataa
      901 tcagacaaac ctgagacaac aatgaccgct cctgctatat aaaatgactg tctcagcaca
      961 aaataaagat aaatcggcag caaaaaaata cggaatatcg ataaaagatt cggaatcgtt
     1021 tgtatttctt tacgccagtc tgtgcttaat ttcattttat cgccctacct cctcagcact
     1081 cttttctttt tattttagct tacacgggga ataaaaccaa tgatttcact gtttttattt
     1141 ttctttacag acactgatat tatgagctaa tttgagataa tcacttgctg ttttcatcgt
     1201 ttactcttct ttcttcctgt cgtttgcgca tttcttttac tagtacttta tgagcttttt
     1261 ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt ataatcgata aacagactgt
     1321 ctaaatcgta acctgtcggg tacagatctt ctgctgaacg caccagacga acacgctgac
     1381 gaggcacgtg gattattttt ttgtctaaat atacatcaat cgtatcttca cctatatctt
     1441 cataaactaa cgcttctttt tggtattcat tgacaaatat ccgatcacct tttgcaaaga
     1501 gctgacgttc ttttttattc tccttcctat ttgtaactgc tgtcttagga agaaattctt
     1561 ttttctgtgt ccgataggct ttttccgcca agtaacgctc tgcttgatgg atcaatttca
     1621 tcgacatatc cattttatga gcaatccaca aagcttggct ttctcctgtt tcttccaacc
     1681 cgcaactgat atttaggacg aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa
     1741 tcttcatgat ctccggcgaa tttcttgatt tctccataat gggtcgtggc aatgatcaat
     1801 gccccttgct catacataga ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc
     1861 tctgttccac tgccaatctc atctaaaagt accaacgtat tcctttttat ctttttcaaa
     1921 atagcagcga tgttttgcat atgaccagaa aatgtactca acgcattttc taaattttgg
     1981 tggtcaccga catcgacgaa aatctcatca aagacagcaa gctctgtccc ttcttttgcc
     2041 ggaacttgca taccgaacat cgccatcaaa gtcaataaac cgactgtctt caacacgact
     2101 gttttgcctc cggcatttgc tccggtgata atcaagcctc gatagtcttt accgatttca
     2161 acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt gataatgata
     2221 tgttccgatt tattgattct aggtgtgctt ccattgattt ctcggctaaa ttttgcccga
     2281 gcaaagatga tatctaacac cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt
     2341 tctgcgatag caccagtcaa tgccgccagg acttgatatt cttcactgat ttcttcagct
     2401 tttaacaact gatagtgttc attcaattta gaaatcacag ttggttcaat aaaaaccgtc
     2461 gttcttttat ttgattcatc gataatcgtt ccatctattt tatttttata gctggctttg
     2521 ataggaatcg tatagtattc gcctttttga acaatcattg cttcttggat catttctttg
     2581 tttttcggat gacgcaagaa tttcagtaat tttgactgga tttctttttc gattagttgt
     2641 aattgtttgc gtactttacg caaattacgt gacgcgtcgt cactgacttt attatttttt
     2701 attttctggt caatcaactc ttctacattt atcaaatcag gaagatgttt gctataagca
     2761 aacaacaagg gtgtctggta ttgattctta tcaaaaaact ttgtgatcat ccgactgctt
     2821 ctaagaaaat ccgcatattc gatcaaatcg gttggctgca gaatcaaccc ttttttgact
     2881 tgttctgtca gtgcatcgat cctcggcaac cccataaatg gaacatgctg actgctttct
     2941 aagatcaagc gtgcttctct tgtttcttct tgacgatcca agacagtttg taggtttgtc
     3001 gagaccgtca tatcgctgat tcgttttttg ctatagtttc caattgctct tgcttggatt
     3061 tcctttttga tccgatcaaa ttgtaattga ctaatcattt cgttgtccat attctctcct
     3121 tttcttgaag agatacctgt cccaaaataa ataagggcct agagataaat aaaaggatag
     3181 aacacagctt aaaaaagtca tgtcctatcc ttattagaat acaaagatat tgttttcctg
     3241 acaggtaccg tttcataagc acaccaaata aactttgttt attggctatt cgacttactg
     3301 attagatacc agctacactc acaaaacata ccccattctt ctaggtctta tttattttcc
     3361 acttaactat agcaagacac attttggttg tcaaacttta gtatgtttta tagatttaca
     3421 ccattaatga atagacacct accgcgataa ttgctccgat cattggacct gcgattggta
     3481 cccatgaata tgcccaatct gaagttcctt tattagctat tggcaatacc gcatgtgcta
     3541 atcgaggacc aaagtcccga gcagggttga ttgcatatcc tgttggccct cctaatgaaa
     3601 gaccgatggc taagatcaat ccacctacag cgaatacgtt cgttccaggt gcaaattcgt
     3661 tttgtccaaa agctagcaac ccaagaacta atacaaatgt accaattaat tctgtgatca
     3721 catttgctgg ataattccgt atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt
     3781 catctttcgt tgcattccaa tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa
     3841 aagctcctaa aacttgcgca atgatgaaag gaacgacaag attccaacta attgcccctg
     3901 tcatcgccat agctagagag actgcaggat tcaaatgagc tgggcccatg aatccagaaa
     3961 tatagacagc cattgttaca gcgagtcccc aaccaaatgc gatgacaatc cagccagaag
     4021 cctgtgcttt acttttgttt aaattcacag ccgcacacac accatctcca agcaaaacta
     4081 ggatcagtgt cccaataaac tcccccatga tttgagtcat atcactgttc atttatttct
     4141 ccccctttag ttcccgcaga tctgattctg caatcaatgc ttctaaacgt ttagtctgtt
     4201 gcgccttctc ctcttctgtc cattcaaaat atccagcaat tgcatcgatc acaggttgct
     4261 tgatctcatc aagctgatct cgttcaaaca acagatgatt tgtacgacga atgagataat
     4321 cgcctggggc caaaaccatt tcttcttcta aaccataacg taaccgagct gactcagcga
     4381 gactcaaacc aggataaggt gccatttctt ttgctaattc aaagatacgt cgggcattag
     4441 tcccgtaaaa atcagcgata taagtagcat cttcttcctc taaaccggct gctactcctt
     4501 cttttgctaa ttctgtcact gtttcttcta atttagttgg atcgaagttt ccacctgaaa
     4561 tctgatattt tttagagtcg atttctttcg tctctattcc gtattcttct tttaataatt
     4621 ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta atcggtgatt ttcccaccag
     4681 ataaagtcag caaaccatct ggttctcttt ctaaagaact acctctggaa atcgtagaag
     4741 gtgctttttc atcacggctg ttttccaaat ggttcaacac gtcttctact tcagcacgag
     4801 aaactttgtt ttccttatat tcacttactg tatcgacaac tttattgaag ctcttgtctg
     4861 aaatcgatcc attatctcca ccattgtaat cagaaccaga attaccaatc aacagtggac
     4921 gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat atttgcttct ggatagcgat
     4981 gattgatcac atccaataga taatctacat cttcttgtgt gactttcgga tccgtaaaat
     5041 ctccttggta atccgtatct gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa
     5101 aaaccatccg cccatcttgc tttcctgtat cgaagtatgt gggttgcggt acaggcagtt
     5161 ttttcgcatc tacaactaaa tggatccctt tagttggacg cattttagga gagactggac
     5221 gcgtaaaatt caagtttcgt actttatcta cccaaggacc actcgtattg atcactaatt
     5281 ttgctttgat ttcgatcact tcatctgtca gcaggtcacg agctttgacg ccaacgattt
     5341 gatcgccatc atataaaaag ccaaccgctt tcattttact tactagataa gccccatctt
     5401 cggcagcctt tttgatatta tcaatcacta aacgggcatc attgttgcgg aaatctagat
     5461 agacacccgc gccttttagc ccttcttttt tcaaaaacgg ttctctttcc aatacttctt
     5521 ctggtgtcag cgtataattc tcatatttgg ttcctgtcac atttgccagt ttgtcataaa
     5581 ggtccattgc tactttgacc gagaacatat tgaaggttgt tgctccttcg ccctcataga
     5641 ttggcaaaag cattggatct ggttttggga tgtgtggggc aatactttgt acgactgcac
     5701 gttcaccaac tgtgtcagct actacttcca catcaaatgt cttcagataa cgaataccgc
     5761 catgaacaag tttggtcgag cgagaggaag tcccttctgc aaaatcctgc atttcgatca
     5821 atcctgtttt gattcctgat gctgctgact ggactgccac accggcacca gtgattccgc
     5881 caccgatgat caacagatcc aattcttctt gctgcatttt ttgaatgcta tcttgtcttg
     5941 tcttgtttga aaacatatgg atttcctcct tattcaaatg cttgggtagc ttttactgct
     6001 tttttccaac cgttgtaaag tttttctcgg cgctcttcct ccatttgaac ttcaaatagc
     6061 ttgccagctt cataaaattc gcggatctcg tctgtatctt tccaataacc gacagctaat
     6121 cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt tttctgctcg ttggatcggt
     6181 acattcaaga tatctgtttg gaattgcatc agatattcat tgtttgctgc accgccatct
     6241 actttcaata caggaatctt gattccagtg tcatcttgca tcgtatcgat gatatctctc
     6301 acttgataag caatcgactg taaagtagct ttaatgatat cttcttttgt cgtaccacgt
     6361 gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg
     6421 aatgcaggaa caacataaac ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa
     6481 tcgctagctt tttgcaacat ttgaagtcca tctctcagcc attgaatcga agaaccagcc
     6541 acaaaaatac ttccttctaa agcatagtaa acctttccat ttatgccgta accaattgtc
     6601 gtcaataaat tatttttgga taattgcggt ttttcaccgg tattcatgac aataaatgaa
     6661 cctgtaccat aagtattctt gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg
     6721 gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa atgataacct
     6781 tgcgtataac cataaacttc tgagttagaa gtgacttttg gtaacatcac tcgaggaata
     6841 tttagtaaat ctaaaatctc ctgatcccaa tctagctgat ggatattgaa caacatcgtc
     6901 cgactggcat tcgagtaatc agtaacatgt gcttgtccat cggtcagttt ccatactaac
     6961 caagaatcga tcgttccaaa catcaattcg cctttttcag ctcgttcttg cgcgccttct
     7021 acatgatcaa gaatccagcg tatcttcgtt gctgagaaat aggcatcgat cactaaccct
     7081 gttttttcgt ggaaaaattc ttgatagcct tcatttttta agttatctgc aattccagct
     7141 gactgtctgg attgccaaac aactgcatga tagattggac gtcctgtatc tttttcccat
     7201 attactgttg tttcacgctg gttcgttatg ccaatcccag caatctgatt tggtttgatc
     7261 cctgattcga tgaacgctcc ggcaataact gactgtacag aattccagat ttcatttgca
     7321 tcatgttcca cccaactttc ttgcgggaaa tattgcgtga actctttttg agaactgccg
     7381 atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc tagttgtacc ttggtcaatc
     7441 gccatgatat acgttgattc tgtcattgtg attccctcca ttatatgcaa acgctttctt
     7501 taactactct tatcatacta ctgttttaaa aaaagaaact gtcaaaattt tattcttttt
     7561 ttcttttttt gacagttttt tattatgaag ctatttttta atcaattctt ttcttttttc
     7621 agcgtctcta ttcttctttt caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga
     7681 taatattcac actgtttttt gtttctctgg caaaatgtga tcaccaaatc atattcttta
     7741 ccttcttcat aacttccaat ctcaataccg ccgattcccc tcagttctct gatcagatat
     7801 tgttgaaaag caatacgata aatcggcaga ctttctaaat cgattccaat cactactggt
     7861 tttgcaatat aaaaatcgat catcaaaaga acattggcat actggatcat caggtaacta
     7921 agctcactat cttcagctct tttcctcggc aattgctgca ccgctgtttc ctgtaatttc
     7981 gccagtaact gtgttaagga atgcccgagc attttctgct gctgttccaa aagccgatca
     8041 cgctcataga cttcgatctt tccaacaaag aaaaaccatt cattgttgat ctgggaaatt
     8101 tggtagccta ctgccttttc ttctttgatt ttcaaacgac gaggacgata atgccataac
     8161 atcgtttccc gaatgtaagt atctaaaaca gcggttggta actttttgct ccgtgtcaaa
     8221 tcatactgat aaaatatttc ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac
     8281 atcattgttt cgtatacatt cagttctgct gctgttcgac taagatgcat cacgatgatt
     8341 ggatcgattg cttgataaag ccggtccgac tgatagagta atttcttttc tttcaaagtt
     8401 gcgtacgtac ttttttcatt caatagtctt tttttcgtaa tcccgagcca gcaagcaatc
     8461 tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac ggtccaatcc tagaatgaac
     8521 ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg
     8581 aatagttcat aataaaaata tcgtatctgt agctcttctc cgtataattg accgtttttg
     8641 atttgtaatc caaattcctc taagagctgg ttcaattctt tgatctttcg aaaaacagaa
     8701 gactcactga tattaaacgc tgtcgctaat cgaacgatgg atacttgctg atgttctaat
     8761 aaatagacga gcatcttaaa cttcaagctt tcctgcaata aaaacaatag aattttttcc
     8821 aaacttacat cttcgtccaa cacaagcagc gcctgatact catttcgctg tacttctact
     8881 tttttttcca tcattcggcc aatcaattca atatcttcca aatattgatc aaacgaggct
     8941 tttgacagtt ggacatgctc tctcaaacca ttgatagagg ctgttccccc ttgcagaaca
     9001 aggtttcgca aaatcaggat ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc
     9061 ataggcagta ttcgccacct ttgaccacac catatctggc acacggtatt ctggctggaa
     9121 aaatagctca tgttcttcta actgttcaaa cgtccatcct tcttctactg caattgtcag
     9181 ttggtcaaga aggtggaaca tctctttttt gctttggagc tgtgctccaa gaatcttttt
     9241 ttcagaatca aatagaaatt tgagtttaac tttgctttga tttattgctg taagcttgta
     9301 gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc
     9361 tacgcttcct aagaaatagc caaaccactg attgccgact ggacgatagg ttccctgatc
     9421 ctttgtctgt gagccactaa ttgtcttgct gacatttcgt gctgtacgga cagcgttatt
     9481 tactagagaa acatacattt tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc
     9541 aatcgcataa acgtcttttt gagacgtttg caagtagtca tctacccaga tcgttccatc
     9601 atcattcaat gttaattgtt cttcccacat ctgattgtcg ggacgagaat gattagccag
     9661 caaaacaaga tcatagttct cctttctttt ttccgtctct acaatagcgg tttcattttc
     9721 ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt
     9781 tactaacggt tctgtcattt caggatctag ataacgaaaa agaattgact ttcggctttc
     9841 gtaaagatgg atttgttttt ttcttgatgc caacccttca gctatttcga ttcctgcttg
     9901 tcctgcacca ataattgcaa ttttctttgc attcatgatc ttttgctgaa gagccgttac
     9961 gttttgtatc tttttaactg ggtgaataag cgttgattct gcagaaatat ttcgaaactt
    10021 ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa
    10081 aacgactttt ttttctttca atcctattac tgtcttttct gtgtaaacat ggattcgata
    10141 gcggtcagtc agttcttctt tcgtgatcca agaaagttgt ttttcactgg taacctttcc
    10201 ttttaatatc aaattgaacc cgctgggaat ccatcctaac ttcgattgct tttcaatcaa
    10261 atatatttca cttgttggca tcaattggcg aagataaata gccgcatgaa tccctgcaaa
    10321 cgacccgccg acaattacaa tgttcatctg cattcctctt tcttataagg tgttctactg
    10381 ctaacttctc aatcagaaaa cacagtattc ataactcgct ttcaacctta tgatacatag
    10441 cgatgcagca gaatcaaccc tctccatcat accgtgttct aggtctgctt ctcaataatg
    10501 tactagtgga cctaattgaa gagtatcatt tctgaagaga tgtttcaaaa aatgtactct
    10561 tataaaagaa aaaaactcgc cttttacgta cctctgtcca aataggaaac agaaaagaac
    10621 gaaaagacga gttttagcgt gtgtaatgta atagttagaa tagacacatc atttattttt
    10681 aattctttaa tgcttcaaca actgcttcgc ctaattgatg agcagattga ggattttgcc
    10741 ctgtaagaag ccgcccatca atttgaacca cttctgaaaa agctgctcca gactgatagt
    10801 ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg tacttcattc gttgtaccat
    10861 ttgcttcctc ttcttcatta ctgaacccag tcactgtacg tccagctaaa atagattttc
    10921 cagactcatc tgtaactggc aacaatccta caacaccgtg acaaacagct gagaccagcc
    10981 cttcatttcg atagatttgt gaagcgactt tcccaagatc tattgcttct ggaaaatccc
    11041 acattgttcc gtggccacca gcataataaa ccgctgcata atcatttgca tccacatcat
    11101 caggacgtaa ggtatttccc aaggcccgat ttctgaaatc ttcatcttca taataggccc
    11161 aatcgatttc atccatattc aaaacacttt gcggttctaa aggtacatat ccgcctttag
    11221 gactgacaaa atctgcatcg atcccatttt ttactaatac atcataaaaa tgagtcagtt
    11281 cacttaacca caaacctgta ggacgctcga tcttttcgta cttttctaca tttgttaata
    11341 cgatcaatac tttcattttc ccatcctctt tctataaaaa ttgcgttatt gataacgtgc
    11401 tttcaagaaa ttatataatg ggcttggcaa ataaagctct ttatatgctt cttcaaaagg
    11461 tttcgttaca aattcatcct cacggttttc caataatttc tctgcccagt caggatcgac
    11521 gatcatttgc tgtccaattg ctgcaagttc tgcatatttc aatacctgct ctacatcttc
    11581 gcgattgcgg atcccaccga caccaactaa agggatcttt ccttgaatcg tgttatggac
    11641 cacttcaaga atcgatttat cgttgtattt atcagaacga gctattcgat catacatatt
    11701 taatgaaaca tgcagataat ctagttttga ctcacgtaat cgttcaagaa gccagatcgt
    11761 gtcttcaaaa cggatacctg gtgtttcata ttcttctggt gagaaacgat accctacgat
    11821 gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc gttgttaata attcttcaat
    11881 aaaacgataa cgattttcca agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg
    11941 agagaaaaat tgttggatca aatacgtatt tgctccatgc aactcgattc catcaaatcc
    12001 tgattcaatc gctctacgtg tggctttgcc aaatgcttgg attgtttcaa cgatttcttc
    12061 ttcactcatt gctcgtggtg tttccgcatc aggacgtttt gctgcaactg cacttgcaga
    12121 tacaggctgt tctccagata aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg
    12181 aataatcgct tttgcgcctt ctccttggat tgctttcgct agctcactta atcgaggaat
    12241 catttcatca tgagcgatgc tgagttcacc tggccagcct tttccgatat cttggacatt
    12301 tgcagcacca gtaataacag caccaagacc tttagaacgt tgtttataat aagtgatctc
    12361 atcttcggtg atcactccat tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa
    12421 tcggttcttc aattgaactc ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt
    12481 cattcctccc ataattcgct ctgattaacc tatcgagaat catctatcat taaaccactc
    12541 gattagttgt acatgcaact aatttgctta aaaaatttta ttttttttgt aacttatctg
    12601 ttgcatcatt cattgtctgg gctaattctt tacttgcttc atttcccaaa actgctttgt
    12661 atgactgatg aagttcttcc cagctaccat cgacttcctt agcaaattgg cgtccttgtt
    12721 ctgtaagata aactaatgac aagcgacctt cgctaactgt tttgacgtat cctttatatt
    12781 ctaatttttc aacaaaacga gcaatcgttg atggggcgga atagagcatt tgtgcaatat
    12841 ctttttgtgg tattccatct ttttctttta atatcaataa aataaaacca taagttggtg
    12901 ccagacctgt ccttctaaac gcttcatcag ctaatttatt caacatgcga tctagctttt
    12961 tcacgctaaa gaaaagacat tcatctaacg ctttttcaat tggcttttcc atttcatccc
    13021 ctccattggc gtacatatca tagtatgatt tttatttttt gtcaaaaaac acggaataat
    13081 gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc atcttcaaag aatttcggct
    13141 tgttttcgac aaaactcctt ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta
    13201 gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
    13261 cttaataccg aaaaactggc ttttgaacac cgtttatatc agaggttgtg aaaaagccgt
    13321 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt gtcgtttttt
    13381 gagctaatgc cgatggtcag ctgattgtac accgtttatt gagaggtcgt gagcctgccg
    13441 ttgaactccg agtcacaagg agcaatttca aaaaacagct tctcatgttt tatgactatg
    13501 gtgacttgtg atcgaggagt tggcaggaga atactgttta ttcgtaaaga aggggctgtg
    13561 acaagacttt tgtcacagtc ccttatttcc ttttattata ggatttcgcc atttgattgg
    13621 atgacttgtc tgtaccaatc aaatgatttt ttctttaacc gtttctttgt gccattccct
    13681 tcgtcatctt gatcaacata gatatatcca taacgttttg acatttgtga agtagaagcg
    13741 ctgacgatat caatgcagcc ccacgacgta taaccaaata aatctactcc atctttgata
    13801 gcttccttca tctgctcaat atgctgcttc aaatagtcaa tgcggtaatc gtcttgaacg
    13861 gttccatctt cttctacttt gtctatagct ccgagtccgt tttcagctat gaacagtggt
    13921 ttttgatacc tatcatataa ttgattcaaa gcaattctca aacccttagg atcaatctgc
    13981 cagccccatt cacttgatgg aagatacgga tttttaattc ctccagttat taagtttcct
    14041 tggacccctt cccaattttc tggatgagca ctgactgcac tagacatgta gtagctaaaa
    14101 gaaacaaaat caacagggta ggctgctaag atttcttcat cacctacttc tatttttaag
    14161 ttgacacctt tttcttcaaa cattcgttgc gtatacgctg gataatatcc tcttgcttga
    14221 acatcactaa agaacaaaga agttctttgc tgctcaattg ttttttgaat atcatccgga
    14281 tcacaagaat acggaacaag aagctgatag ctgatcatac aaccgacttg tgaacctttg
    14341 ataatttcat gacaagcttt tgtagctaaa gcacttgcta taaactgatg gtgtgcacct
    14401 tggtatttcg cttgttccaa atggggatgg ttttcttcaa ttactcctac gctaacaaaa
    14461 gggtgatgtt ttacacagtt gatctcattg aaagtcatcc aatattttac tttattttta
    14521 tagcgtttga atactgtttc agcgaaacga acgtaaaact gaataagctt tcgattaggc
    14581 catccaccat attctgtaac caaatataac ggcatttcat aatgggaaag agaaacgact
    14641 ggctcaatcc catattttag acattcgtca aacacttgat cataaaaaaa taatcccgct
    14701 tcattgggtg tttcttcatc accatgagga aaaattcttg tccatgcgat agacatccga
    14761 tagcatttaa aacccatttc agcaaataaa cggatgtctt ctttaaagtg atgatagaaa
    14821 tcatttccgt gtcttttagg ataataatac ttgctgttgg gatcctgcgc cgcttttact
    14881 tgctgatgcg tcatcatatg ccattgatct gtccattttt ctttaggtaa atcgctgtcg
    14941 aatgtataca catcagacac agataatcct ttttcatcta catcccatgc accttcacat
    15001 tgattggccg ctgtcgctcc gccccataaa aatccttctg gaaatttttt catatcgatc
    15061 cccctaaact tgctagtttt cttgtttgat tatttttctt atgactagta atgatatttt
    15121 tgaatctact actatttgta acaataacca ttgtggtcgg atcatatccc tctttgatta
    15181 attcttgcaa atctacttcc atcaataaat cgcctttacg tacttttttt ccatcagata
    15241 ctgcactttg aaagtatttg ccatttaaat tcactgtatc aataccgata tggatcagta
    15301 tctctgctcc ttcttcactg actaccccaa ttgcatgttt cgttggaaaa actgttgaaa
    15361 cttccccgtt tacagaactg tagatttttc cgtccgttgg aatgatcgca aacccttctc
    15421 ctaaagatcc actagcaaat gttggatcgt ttacttcaga taaatcaatt atttcacctg
    15481 ctgcaggagt acttaacact atatcctttg aggattcttc ttcgatttca tccttgtagc
    15541 cgaaaagaaa agttagcata caggaactga tcatcgctaa gaaataacta gcaactagcc
    15601 aaacaaatcc ttcaccaata taaatcggga aagacaaaat acttcttgta gcaactgcca
    15661 aagcaccggc tcctgcggca ccagctactc caccaaatat agctccacat atacaagcaa
    15721 tgtaaaatgg tcgtttatat ttaagagtca caccataaac agcaggttca gtgattccaa
    15781 ataatgctgt gataaatgca gataaagata aactttttac tttcttattt ttcgttttca
    15841 aaaatacccc aagtactgct cctgcttgcg cagctacggc agtcatacat gctggaccta
    15901 gcgtatcacg accgtagaaa gaaagattgt tggtcattac tggtacaagt ccccaatgta
    15961 acccaaaaat cactaacact tgccaaaatc cgccgataac agcaccagat aaaacagggc
    16021 taaagttata aacagctaaa tatccagctg caatcagctg acttgcataa tctgcaattg
    16081 gacctacaac taataaaata attgggacca tcacgatgat acaaatgaat ggtgtcaata
    16141 aatttcgaat actttcgtgc aagcttttgc gaatcttagg ctcaatataa gaaagaaccc
    16201 atacagctaa aattgctgga ataaccgtac ttgtatacct tgctagaatc acgggcatcc
    16261 ctagaaatcg caaggttatg ctatcactat atgcactgac aatcgtagga taaacgagag
    16321 ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa cttttttgca gcagtatatg
    16381 ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa atgatataag
    16441 ttcctgattc agtagttaat aaattcaaac tcgtacataa ggcgagtact cctttgatca
    16501 aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc atgtctataa
    16561 aagagtttaa aatgcctttt ttctcagttc ctctctttcc actagatgca tcatcttcta
    16621 gtttcatttg tttggataat acgtcataca cttcatttac tgcatttcct actacgactt
    16681 gaaacattcc tccagcttcg actacagtga tcacaccttc tagttgctct accttctcct
    16741 tatctgcctt cttattatct tttagggtaa agcgtaacct tgttgcacaa tggtaaacat
    16801 tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt
    16861 ccatttttac ttcccccttg ttaattaatg ccgtagcaat ccttcttaca agataagtat
    16921 agttcttaga aaacggtttc tccattcaat tagtacccct tctaatagcg aacgaaactg
    16981 tacaatagtg tgtatggttt acttagccga gcaaatcact cactgcttga aagaaaacaa
    17041 gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct
    17101 gatgtataag tggtatctca agctgatact ctgtgatttt tgtttctact tggcttttga
    17161 taagacagca caacaaagtc tcggcattta acggaagcat aactaggtca gaactaggag
    17221 agattagttg ctgaatggaa tctatctttt tatggatttt tatttgattt tcttctttgt
    17281 agtaatgatc aagcagtttt gttttctttt tttcttcaac tatggtcagc ttttcacgca
    17341 aaatctgata aatctcccaa gattcaaact catctagaac actaggtaga taaatcaaat
    17401 catcccactc aaaaataagc tcttgctcag tagtcaaaat tgcatcatat tccgttattt
    17461 tgtttgattg atttttgaaa agatacactg gttctatccg gcattctttg atccacatgt
    17521 tcatactttg ttgaatctgt ttttttagtg tattcaaaat actaattggc tggttactaa
    17581 caatcagtat ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa
    17641 aataagcgct ttcagctaaa ttgggacgta attgaaaatg tattgggaag aaacgccgaa
    17701 ttaaataaat ttctaaagga taacgggaga tgatcttttg tacaaaatga ttcatggtat
    17761 tatgtccggc atccatcctc gttttcattt gtttgatatg ggttagcaaa aattctgttt
    17821 catggaacaa taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc
    17881 ttagattctt ctctatttct ttgttttttt catcattaat catcaaataa tttaactcta
    17941 atagtcgatt ttcaattgct tgtttttctt gattggaaaa agaatagggt agttctgatg
    18001 ataacttttt tgtgagggtc tctaccatcg gaataaataa tgtatacctg tttgtttttt
    18061 ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat cgtatgaagt
    18121 cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc ttttggacag
    18181 tcggcatgat attggaaaaa attgtactga tttgttgagg aaaccttgcg tgttggatca
    18241 ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca gcaatgattt
    18301 ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg atccaacgca
    18361 aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg tctgctatct
    18421 tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt gaagctcgga
    18481 aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc tgtttagttt
    18541 cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata atgattttct
    18601 tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg ttggtaccta
    18661 aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat actacaagaa
    18721 tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt ataaacatac
    18781 aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa
    18841 gatctatatt caaggttttt accattagac tggataattt gtttataata ttcaaaacta
    18901 tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac ataaataaag
    18961 ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg tccccaccat
    19021 aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg aagatgtatt
    19081 tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg tgcttcatct
    19141 agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt caattcattc
    19201 atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg agatttttcc
    19261 ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat ttctgcttct
    19321 tttttataga cagcgctacg ataataacta aatgcaatat aatctgccgt gttagcttgc
    19381 aagcactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt ccatgtcctg
    19441 taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca tgaattgcgt
    19501 ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata agtcgctaat
    19561 gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt taataattga
    19621 gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatcac tttttttgtt
    19681 agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc agcagctgca
    19741 taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt cactttccct
    19801 tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt
    19861 tgccaccccc catactctcc taccaatgct aagggggttt cataatgact aagagtaatt
    19921 actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa tacgagacca
    19981 gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga gatgcttgtt
    20041 cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa tcgatgataa
    20101 aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat ctcccaaatc
    20161 tttttttctt cattccactg aatactagga tgctggttca tgatcccgat aagcgtatct
    20221 gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt agcagctact
    20281 gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat ctctcctcgc
    20341 tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata ttctaactat
    20401 ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat ttatatataa
    20461 caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc aaacgattta
    20521 ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt ctggtaactg
    20581 tttcagagga taatttgaca caataaccct taaagcttca cgtggaatag tgaacgtctg
    20641 cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata tgcgagaata
    20701 ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga cgatgatgtc
    20761 atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat cttgatctgc
    20821 taacgcttct tctgcattga tttctttata atttggattg actcgaagcc atggaattcc
    20881 agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag cattgtctct
    20941 tcctttcgcc ttgattgatt ccaagatttc ctcttttgta aatccagaag aaatcctttc
    21001 gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga ttgggcaatt
    21061 cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca tatggagcaa
    21121 gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc aacgagaaac
    21181 aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat cacctaaaga
    21241 tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt tcaaatccca
    21301 tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga ccatggataa
    21361 ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc cccatgtttc
    21421 gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct gaagatactc
    21481 atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa tcatatcgat
    21541 cacgtccata cgaaagcccc caataccttt atcgatccag aaattcatca tttgataaat
    21601 ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc tatacaagtg
    21661 cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga atattgagcg
    21721 caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt cccgataagg
    21781 attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag tatgattcac
    21841 caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca gttcttccat
    21901 ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat catacccatt
    21961 gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac ctaaatacgc
    22021 cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt gatttgaatc
    22081 ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt tttgttccat
    22141 gattttcctc ctataaatgg atgtttctat tttttgctta atcatttatt ttagtctctc
    22201 tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct tgttatcggt aacagaataa
    22261 aacaaaaaaa cagctgagga aattccctca actgtttttt gaaaggattg atttatgcag
    22321 ggaagaatgc tgatttcaca acttctttat aaacggtgtt cactcagctg tcccttggta
    22381 ttagctcaaa aaacggcaaa atataagaaa ctattttttc gtttttttct ctaatactca
    22441 ggccagaacg ctgatttcac aacctcttta tttcacattc aatatccaca tgaatcccac
    22501 aaatacaaag aacagaccat acatgattgg agatacttct tttcctcttt tagctgctaa
    22561 catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac tataagtcaa
    22621 aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc cccattcgat
    22681 ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg cagttacttg
    22741 tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga aaatccctgt
    22801 agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg attctacaaa
    22861 tgcaccaaca ggagaagtac caaataagga acccgcaagc atagctgttg aatcagaagc
    22921 taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa
    22981 tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca gcactacgac
    23041 ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa atgttggttt
    23101 caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg tgaccagacc
    23161 taaaatcgtt gtagctgcca tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa
    23221 gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc
    23281 taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca gaccaaggaa
    23341 agcgataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg ggatcgcatc
    23401 aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa
    23461 tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag aataagcaaa
    23521 aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta atacccccat
    23581 taaaacacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc cttcatccat
    23641 tccagaagca cctaatacag ttggatttac aaataagata taagccattg agataaatgt
    23701 tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta tttcgaagta
    23761 ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata aataaagtcc
    23821 gttaaaattt cgatttatta ataaaacaac gtgttttatc atatactaaa acaaaacata
    23881 agtaaacaaa aaattcggat tttaagcaaa atccgaacgt tccatctgat taaatccctt
    23941 tatctcaaag ttcatgaaca ttcactttgg attacaagaa tttctttatt gtgttaataa
    24001 agtttcttca ttctagtctc aaaaatcaat agtgtgattt gattttacca tttcgtttct
    24061 gttcttcttt gatgaattct tttgtatcga caggattcag attatttagt ccgccaaaac
    24121 ctgtatcgac catttttccc cagccttttg tcttcttttt tcctgatttg agattctttg
    24181 ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa ataaccttct aatcttccca
    24241 ttagatccac tgtttttcct cctttttatt caatcagacg actcagcttt tcctataaca
    24301 aaataattgc cttcagggtc agaaaaattg aaagtcaaga tgccattgtt atctgtgatt
    24361 tctcctggtt cttccatgtt ctcgtagata gtttcaaatt catccgtaaa aaatactaaa
    24421 gaaggcggtg gtcctaatac ctctggtgaa aacttctgca caaatacttt tgggaatacg
    24481 cctaattcta attcttttga gatgctgagt acaatactct taaattcttc tggcaattcg
    24541 attgtttcag aaatttctgc accaattttt tctacaaaga atcggcaaat catctctacg
    24601 tcattgacat aaagcatcac acgtgttttt tgtatcatca tttgaccctt ctttctattt
    24661 gattttcttt cctgtctata aactgattcc attatatcac gaaagcgcaa acaccttgtg
    24721 tttgatacta ttcaagaaaa ataaagcaat agtttctagg attgctttcc tccacaaact
    24781 ttttttacaa aaaaaaaacg gcaatgaatg ccgct
//
LOCUS       DASJST010000030        24374 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJST010000030 DASJST010000000
VERSION     DASJST010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.030"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(63..911)
                     /locus_tag="U4B05_000953"
     CDS             complement(63..911)
                     /locus_tag="U4B05_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2050106.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="U4B05_000954"
     CDS             complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="U4B05_000954"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN2050107.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3412..3591)
                     /locus_tag="U4B05_000955"
     CDS             complement(3412..3591)
                     /locus_tag="U4B05_000955"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN2050108.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3638..5461)
                     /gene="cadA"
                     /locus_tag="U4B05_000956"
     CDS             complement(3638..5461)
                     /gene="cadA"
                     /locus_tag="U4B05_000956"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2050109.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5739..8018
                     /locus_tag="U4B05_000957"
     CDS             5739..8018
                     /locus_tag="U4B05_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2050110.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8111..8758)
                     /locus_tag="U4B05_000958"
     CDS             complement(8111..8758)
                     /locus_tag="U4B05_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050111.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8937..9656)
                     /locus_tag="U4B05_000959"
     CDS             complement(8937..9656)
                     /locus_tag="U4B05_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2050112.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9787..10938)
                     /locus_tag="U4B05_000960"
     CDS             complement(9787..10938)
                     /locus_tag="U4B05_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN2050113.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11155..11895)
                     /locus_tag="U4B05_000961"
     CDS             complement(11155..11895)
                     /locus_tag="U4B05_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2050114.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="U4B05_000962"
     CDS             complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="U4B05_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN2050115.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="U4B05_000963"
     CDS             complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="U4B05_000963"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN2050116.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12856..13506)
                     /locus_tag="U4B05_000964"
     CDS             complement(12856..13506)
                     /locus_tag="U4B05_000964"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN2050117.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="U4B05_000965"
     CDS             complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="U4B05_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN2050118.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="U4B05_000966"
     CDS             complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="U4B05_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN2050119.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14949..15476)
                     /locus_tag="U4B05_000967"
     CDS             complement(14949..15476)
                     /locus_tag="U4B05_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN2050120.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15656..16441)
                     /locus_tag="U4B05_000968"
     CDS             complement(15656..16441)
                     /locus_tag="U4B05_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN2050121.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16434..17303)
                     /locus_tag="U4B05_000969"
     CDS             complement(16434..17303)
                     /locus_tag="U4B05_000969"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN2050122.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17304..18683)
                     /locus_tag="U4B05_000970"
     CDS             complement(17304..18683)
                     /locus_tag="U4B05_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN2050123.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="U4B05_000971"
     CDS             complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="U4B05_000971"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2050124.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19135..19611)
                     /gene="accB"
                     /locus_tag="U4B05_000972"
     CDS             complement(19135..19611)
                     /gene="accB"
                     /locus_tag="U4B05_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN2050125.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="U4B05_000973"
     CDS             complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="U4B05_000973"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN2050126.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="U4B05_000974"
     CDS             complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="U4B05_000974"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN2050127.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="U4B05_000975"
     CDS             complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="U4B05_000975"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2050128.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22641..22865)
                     /locus_tag="U4B05_000976"
     CDS             complement(22641..22865)
                     /locus_tag="U4B05_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2050129.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22918..23880)
                     /locus_tag="U4B05_000977"
     CDS             complement(22918..23880)
                     /locus_tag="U4B05_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN2050130.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23890..24330)
                     /locus_tag="U4B05_000978"
     CDS             complement(23890..24330)
                     /locus_tag="U4B05_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2050131.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6666 a         5425 c         4250 g         8033 t
ORIGIN      
        1 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
       61 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
      121 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
      181 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
      241 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
      301 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
      361 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
      421 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
      481 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
      541 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
      601 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
      661 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
      721 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
      781 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
      841 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
      901 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
      961 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     1021 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     1081 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     1141 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     1201 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     1261 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     1321 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     1381 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     1441 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     1501 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     1561 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     1621 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     1681 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     1741 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     1801 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     1861 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     1921 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     1981 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     2041 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     2101 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     2161 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     2221 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     2281 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     2341 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     2401 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     2461 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     2521 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     2581 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     2641 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     2701 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     2761 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     2821 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     2881 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     2941 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     3001 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     3061 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     3121 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     3181 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     3241 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     3301 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     3361 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     3421 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     3481 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     3541 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     3601 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     3661 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     3721 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     3781 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     3841 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     3901 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     3961 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     4021 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     4081 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     4141 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     4201 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     4261 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     4321 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     4381 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     4441 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     4501 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     4561 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     4621 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     4681 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     4741 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     4801 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     4861 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     4921 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     4981 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     5041 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     5101 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     5161 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     5221 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     5281 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     5341 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     5401 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     5461 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     5521 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     5581 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     5641 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     5701 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     5761 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     5821 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     5881 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     5941 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     6001 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     6061 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     6121 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     6181 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     6241 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     6301 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     6361 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     6421 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     6481 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     6541 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     6601 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     6661 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     6721 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     6781 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     6841 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     6901 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     6961 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     7021 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     7081 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     7141 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     7201 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     7261 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     7321 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     7381 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     7441 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
     7501 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
     7561 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
     7621 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
     7681 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
     7741 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
     7801 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
     7861 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
     7921 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
     7981 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
     8041 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
     8101 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
     8161 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
     8221 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
     8281 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
     8341 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
     8401 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
     8461 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
     8521 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
     8581 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
     8641 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
     8701 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
     8761 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
     8821 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
     8881 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
     8941 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
     9001 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
     9061 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
     9121 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
     9181 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
     9241 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
     9301 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
     9361 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
     9421 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
     9481 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
     9541 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
     9601 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
     9661 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
     9721 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
     9781 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
     9841 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
     9901 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
     9961 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    10021 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    10081 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    10141 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    10201 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    10261 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    10321 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    10381 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    10441 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    10501 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    10561 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    10621 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    10681 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    10741 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    10801 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    10861 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    10921 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    10981 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    11041 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    11101 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    11161 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    11221 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    11281 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    11341 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    11401 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    11461 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    11521 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    11581 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    11641 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    11701 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    11761 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    11821 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    11881 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    11941 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    12001 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    12061 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    12121 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    12181 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    12241 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    12301 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    12361 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    12421 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    12481 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    12541 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    12601 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    12661 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    12721 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    12781 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    12841 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    12901 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    12961 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    13021 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    13081 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    13141 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    13201 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    13261 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    13321 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    13381 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    13441 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    13501 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    13561 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    13621 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    13681 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    13741 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    13801 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    13861 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    13921 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    13981 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    14041 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    14101 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    14161 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    14221 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    14281 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    14341 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    14401 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    14461 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    14521 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    14581 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    14641 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    14701 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    14761 caaaaagtcg tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa
    14821 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    14881 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    14941 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    15001 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    15061 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    15121 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    15181 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    15241 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    15301 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    15361 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    15421 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    15481 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    15541 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    15601 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    15661 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    15721 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    15781 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    15841 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    15901 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    15961 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    16021 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    16081 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    16141 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    16201 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    16261 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    16321 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    16381 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    16441 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    16501 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    16561 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    16621 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    16681 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    16741 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    16801 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    16861 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    16921 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    16981 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    17041 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    17101 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    17161 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    17221 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    17281 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    17341 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    17401 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    17461 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    17521 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    17581 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    17641 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    17701 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    17761 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    17821 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    17881 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    17941 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    18001 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    18061 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    18121 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    18181 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    18241 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    18301 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    18361 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    18421 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    18481 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    18541 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    18601 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    18661 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    18721 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    18781 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    18841 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    18901 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    18961 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    19021 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    19081 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    19141 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    19201 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    19261 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    19321 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    19381 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    19441 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    19501 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    19561 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    19621 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    19681 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    19741 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    19801 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    19861 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    19921 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    19981 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    20041 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    20101 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    20161 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    20221 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    20281 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    20341 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    20401 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    20461 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    20521 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    20581 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    20641 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    20701 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    20761 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    20821 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    20881 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    20941 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    21001 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    21061 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    21121 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    21181 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    21241 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    21301 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    21361 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    21421 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    21481 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    21541 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    21601 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    21661 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    21721 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    21781 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    21841 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    21901 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    21961 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    22021 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    22081 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    22141 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    22201 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    22261 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    22321 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    22381 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    22441 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    22501 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    22561 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    22621 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    22681 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    22741 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    22801 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    22861 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    22921 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    22981 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    23041 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    23101 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    23161 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    23221 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    23281 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    23341 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    23401 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    23461 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    23521 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    23581 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    23641 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    23701 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    23761 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    23821 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    23881 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    23941 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    24001 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    24061 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    24121 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    24181 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    24241 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    24301 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    24361 caaatctttt gatg
//
LOCUS       DASJST010000031        24061 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJST010000031 DASJST010000000
VERSION     DASJST010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.031"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(119..565)
                     /locus_tag="U4B05_000979"
     CDS             complement(119..565)
                     /locus_tag="U4B05_000979"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN2050132.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(576..2789)
                     /locus_tag="U4B05_000980"
     CDS             complement(576..2789)
                     /locus_tag="U4B05_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN2050133.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(3022..3786)
                     /locus_tag="U4B05_000981"
     CDS             complement(3022..3786)
                     /locus_tag="U4B05_000981"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN2050134.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3787..4734)
                     /gene="prmA"
                     /locus_tag="U4B05_000982"
     CDS             complement(3787..4734)
                     /gene="prmA"
                     /locus_tag="U4B05_000982"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN2050135.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4747..5235)
                     /locus_tag="U4B05_000983"
     CDS             complement(4747..5235)
                     /locus_tag="U4B05_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN2050136.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5201..5872)
                     /locus_tag="U4B05_000984"
     CDS             complement(5201..5872)
                     /locus_tag="U4B05_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN2050137.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6010..7296
                     /locus_tag="U4B05_000985"
     CDS             6010..7296
                     /locus_tag="U4B05_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN2050138.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7368..7562
                     /gene="ssrS"
                     /locus_tag="U4B05_000986"
     ncRNA           7368..7562
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4B05_000986"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7640..7924
                     /locus_tag="U4B05_000987"
     CDS             7640..7924
                     /locus_tag="U4B05_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050139.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7997..8476
                     /locus_tag="U4B05_000988"
     CDS             7997..8476
                     /locus_tag="U4B05_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2050140.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8699..8842
                     /locus_tag="U4B05_000989"
     CDS             8699..8842
                     /locus_tag="U4B05_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN2050141.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9420..11003
                     /locus_tag="U4B05_000990"
     CDS             9420..11003
                     /locus_tag="U4B05_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2050142.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11440..12624)
                     /locus_tag="U4B05_000991"
     CDS             complement(11440..12624)
                     /locus_tag="U4B05_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN2050143.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12649..13656)
                     /locus_tag="U4B05_000992"
     CDS             complement(12649..13656)
                     /locus_tag="U4B05_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2050144.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13828..14187)
                     /locus_tag="U4B05_000993"
     CDS             complement(13828..14187)
                     /locus_tag="U4B05_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050145.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14159..14530)
                     /locus_tag="U4B05_000994"
     CDS             complement(14159..14530)
                     /locus_tag="U4B05_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN2050146.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(14547..14849)
                     /locus_tag="U4B05_000995"
     CDS             complement(14547..14849)
                     /locus_tag="U4B05_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050147.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(14866..15336)
                     /locus_tag="U4B05_000996"
     CDS             complement(14866..15336)
                     /locus_tag="U4B05_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN2050148.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15311..15589)
                     /locus_tag="U4B05_000997"
     CDS             complement(15311..15589)
                     /locus_tag="U4B05_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN2050149.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15586..16635)
                     /locus_tag="U4B05_000998"
     CDS             complement(15586..16635)
                     /locus_tag="U4B05_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN2050150.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16592..17521)
                     /gene="tadA"
                     /locus_tag="U4B05_000999"
     CDS             complement(16592..17521)
                     /gene="tadA"
                     /locus_tag="U4B05_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN2050151.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17653..19407
                     /gene="ade"
                     /locus_tag="U4B05_001000"
     CDS             17653..19407
                     /gene="ade"
                     /locus_tag="U4B05_001000"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN2050152.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19505..20167)
                     /gene="trmB"
                     /locus_tag="U4B05_001001"
     CDS             complement(19505..20167)
                     /gene="trmB"
                     /locus_tag="U4B05_001001"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN2050153.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20237..21019)
                     /locus_tag="U4B05_001002"
     CDS             complement(20237..21019)
                     /locus_tag="U4B05_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN2050154.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21223..22434)
                     /locus_tag="U4B05_001003"
     CDS             complement(21223..22434)
                     /locus_tag="U4B05_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050155.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22427..23164)
                     /locus_tag="U4B05_001004"
     CDS             complement(22427..23164)
                     /locus_tag="U4B05_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050156.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23302..23736
                     /locus_tag="U4B05_001005"
     CDS             23302..23736
                     /locus_tag="U4B05_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN2050157.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23729..>24061
                     /locus_tag="U4B05_001006"
     CDS             23729..>24061
                     /locus_tag="U4B05_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2050158.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYP"
BASE COUNT         7146 a         5080 c         4294 g         7541 t
ORIGIN      
        1 ttccgactgc tgacaaaaga agcgcttttt gcagacaaaa aagccactta agttagagtt
       61 tatcagtcta acttaagcga cgtgtttcat agtttttact agtgaaaacc agattacttt
      121 atttgttttt agaatcaata atgatcgtta caggtccatc attgacgaga gaaaccgaca
      181 tatctgctcc gaattgtccg gtagcgactg tgatgccgcg acttctgatt ccttcattga
      241 atgattcgta taaaggaata gctgtttctg gtcgtgcggc ttccacaaag cttggtcgat
      301 ttcctttttt cgtatccgca aacagtgtga attgagagat acttaaaatt tgtcctccca
      361 catcttctac actaatattc atttttcctt gttcatcttc aaaaatacgc atttgtgcaa
      421 ttttttttac cagatagtcc acatcttttt gcgtatcggt ttcatgaatg cctaataaaa
      481 caagtaggcc gtagccaatt tttccaattt cttgctgatc gactgacaca gcagctttac
      541 ttacccgttg aacaactgct ctcatatctg tcctcctatc cattcgtccg gcgtacactg
      601 tagacatcag gtactgcttt gattttatcc acgatactct ttaggtggga taaattctgg
      661 ataccaaccg tcaaatggat cgtcgccatt ttatctttat tcgtccgtgc ttcaacgctg
      721 ttgagatttt tcgttagcgc attgactgtt tgaagtacat cattcaataa gcctgaacga
      781 ttatagccgt aaatttccaa atctgcatca tactcttttc gtgtattcga tgtatcttcc
      841 cattcgactt caatcaaacg ttctgctaca tttggtttgt caggctgaac attcggacaa
      901 tctcgacgat gaatggatat ccctctgcct ttagtgatat aaccaacgat atcatcacca
      961 ggaatcggat tacagcagcg actgatacga attagtaagt tttctacccc ttgaatgaca
     1021 acgccacctt catgacgtac tttcattttg tcaggttctt ttttcggctg attcatgatt
     1081 tcttctgctt cttgcttttg ctgctcgatt ttttgttctt tacgttcttt ttcagtcaga
     1141 cgattggcca tcgtcaaggg gctaacttct ccatacccta cagctgcata gagatcatct
     1201 tctgtttgat aattaaaacg atcgagtgct tcttgcagtt tgttcttcgt caaaatatct
     1261 ttaggcgtaa atcctagatc tgtaatacat ttgaccacgg attcgtggcc tttgatcaca
     1321 ttttcttctc gatcttgggc tttgaagaaa cgtttgatct tatttcttgc tttgctagta
     1381 gcaactaatt tcaaccagtc gcgacttggg ccaaatgaat ttggagaagt catgatctca
     1441 atgatatctc cgtttttcaa tttgtaatca agctgcacca ttttgccatt tacttttgca
     1501 ccagtggttt tgttaccgat atctgtatgg atactgtatg caaagtccaa tggtccggat
     1561 ccttttggta actcagtaac gtctcctttt ggtgtgaaga cgtagacttt atcactaaag
     1621 atatctcctt ttacgccttc cataaattca gaagcatcat agctttcgtc ttgaagttcg
     1681 agtatctcat ggaaccagct taattgtttc gtcataccat ctggttctac tttttcattt
     1741 tttccttctt tataggccca gtgtgcagca accccgaatt cagcgatttc atgcatttcc
     1801 tgtgttcgga tttgaatttc taccggattt cctgccggac cgatcacggt agtatgcaaa
     1861 gattggtaca tattagcttt tggcatggca atataatcct taaatctgcc gggcattggt
     1921 ttccattttg tgtggattgc tcctaataca gcataacaat ccttgattga atctacgatc
     1981 actcgaatag ctagcaagtc ataaatttcg ttgaactgct ttttctgatc tttcatttta
     2041 cgatagattg aataaatgtg ttttggccgt ccatagattt ctgcaaaaat ccccagctct
     2101 tctgtcgcta ttcgaatatc ttcaacagta ccacttacgt atttttcacg ttcttctctc
     2161 ttcgtctgca tcaagtggac gatacggtaa tactgttttg gatttagata acgaagtgct
     2221 gtatcttcta attcccattt gatccggctg atccctagac gatgtgcaag aggcgcatag
     2281 atttctaaag tttcctgagc gatccttctt tgcttatctt ctcgcaggtg cttcaacgta
     2341 cgcatgttat gtagtctatc agctaatttg accatgataa ctcgtaaatc ttgagccatt
     2401 gctagcagca tctttcggtg gttttctgct agctgctctt cgtgggattt atattttatt
     2461 ttccctaatt tggttacgcc gtccactaac atagcaacgt catcgccaaa ttcttctctc
     2521 agatcttcta aagtgatttc tgtatcttca acaacatcat gtaagaagcc tgtagccact
     2581 gtatggggat ccatttttaa atctgctaat atacctgcga cttggatagg atgaataatg
     2641 tagggttcgc ctgattttct aaactgtccg tcatgtgcag cagtcgcgta ttcgcatgct
     2701 ttctcaacaa atgctacatg ttcgggaccc atgtattgac taacgagctt tatgacaccg
     2761 ggtcccgtta aaatttcttc ttttgtcaca ggaatcgatc cccctttctt ttttcggtaa
     2821 aacgggaatc gggacaaata tctgtttgat aattgtccca aaatccgtgc agttatacgt
     2881 ttttttacgg aaatgttcgc tttttaaaag ctttttcaaa cgatttttcg tatccattgt
     2941 ataaataatt gtttctatta tacaaattgt tgactatttt ttcaattctt tctgtcgata
     3001 ctcttttatt aatgaagaac gttagctgaa agaaggattc attaattccc attgatagct
     3061 agcagcactt aataaataaa gaggtgctgt ttctgtacga aggattcttg gtcctaatcc
     3121 acaaagtatc cctttgtgtt ctacaaaata atcgacttca tcgcgggata atccaccttc
     3181 tggtccaaag acagctaaga ttcgactatc tggttttgtt tcactaagaa ttttggctgc
     3241 atttgatttt tcgccttgct ttgctgattc ctcataagca accaagacca cgtcataatc
     3301 atctagccgc tcaaataaat cttgtttcgt ttccaataac tctactgatg gcacgatctg
     3361 ccgatgggat tgttccgctg cctcttgagc aatcttttca agccgttgtt gtcgtttcat
     3421 acgttttttt gcatcccatt taacgacaga ggctgatgcc gggaaaccaa gaaaagcttg
     3481 cgctcctaat tcagtggatt tctgaacgat ccaatccagc ttttcccctt taggatagcc
     3541 actcgctatg gtaatcgaac ttggcagttc cttttgattt tcttcttttt taatttcttt
     3601 cagataaaca gcctcttcag taatctccgt gatttctgca acaatggcta tttgatcatt
     3661 gaatgccaaa aaaacttcat cctcgggttc catcctcata acccgtacaa tgtggtgata
     3721 gttttcccct tctaaaacaa aatagtcttt tgattcatat gattctttta aaaaataccg
     3781 ctgcatctat tccacctctg gttttttcaa aataattgca taccagtctt tttgctggaa
     3841 aatttgatcc acgataaacc cttgttctaa catggcatcc agaaccattt gcttcttctc
     3901 ttcaatgatt cctgaaacga tcagcgtgcc tgtttgcttc agtaaacgcc aagcatctgg
     3961 gatcatcaat aagatgatat ctgctaaaat gttcgcaacg attacatcac tttcaatctc
     4021 gattcctgtc aaaagatcgt tagctgatac atggacatct ttggctattt cattcatatc
     4081 catattttct ttcgccgaac gaacggctac ttcgtctaaa tcaaaggcat agacatcttt
     4141 tgcaccaaga tattttgctg caatacttag cacgcccgac ccagttccca catctagcaa
     4201 tgtttcacca ccacgcaaga ctgtttctag tgctgtcaat gtcaaggaag tcgtgggatg
     4261 agtgcctgtg ccaaatgcca taccaggatc tagtgtgata attttttcta gaggatcttc
     4321 tgtctggtag ttttcccagc ttggcgcaac cgtcagcatt cgcgtaatac gcaccggatg
     4381 atagtatttc ttccaggctg ttgcccagtt gctttcctct acttcactaa ctgttacttt
     4441 attttttcca atagctagac caaactcagg taattgtgtg atacgatctt tgataaaagg
     4501 caagatttcc ggtaagaaag tcgtttccgg aaaataagcc atgactagtg cgccttcttt
     4561 cagcgatgta aaatcatttt tatctaaaat ctcaccatat tgatccgact gaaaatgttc
     4621 aacatctaaa gaatcttcaa tcgcgacacc actcgcccct gcctccatca aaatattagc
     4681 aactgcttcg actgcttcac tttcagtttc tacttttact tcattccatt tcatatctgt
     4741 cactccttaa taacttgggt acggtaaata agtatctgct tcattttgct gttttacttc
     4801 tactaattct gctagttgct gattatccca gtgaagctcg aatacagctt cgtcatcgtc
     4861 ttcatccaaa aaatcaagca aatcgctttg cccttgagct aatacttcat ttagatattc
     4921 cactaaagaa tcagctactg attttgccaa cccttttttt ccttcataag ggattactgc
     4981 tagataattg cgttcatcaa aaacagattt ctgaggatta aaaagaagta cgccgtcttc
     5041 aaattcgata attggttctt gcgaaacgat cccttctgca tcatcaatct caaattgtgc
     5101 tttattttct gcaaacaagc gaacaacgat ctcgatcgta tgattttttg tatcccaatc
     5161 taaagctaca tcatagtctg tcacttgttt ttcaattatt tcatctaaat atgttaatag
     5221 atccttcttt ttcattttct ttcctccaac cccaatcttt ctcgatttgg ttttttcttt
     5281 gtaaagagat ataaggattt cccgacacaa caaaacgcaa cggcttttct gtccatacac
     5341 ctttgtttgg gataccgatt ctaggcaaag cattgatctt ttttggtgtc ttttttttct
     5401 caaaaactaa gcgtaacgaa ctatctgcta tagactgacc ataaagttct tgaggaattg
     5461 ctaatgcttc cactaatttt cccggaccgt tgctgatatc tggaccagtt tttccgccac
     5521 gatttttact catttgatca atcattgcgg cgggttcgat tgcacgtatc attactcctt
     5581 gaggaatccc ttctggctga gtaatgatgt tcaaaatccg atgagtatgc atcgtgtaaa
     5641 ggtagatcgt acctggtttt tcatacattg cccgcacacg cggtgttctt cttaaaccat
     5701 agctatgggc ggcctcgtct tctggaccta ggtatgcttc cgcgtctaca atatatccag
     5761 ctaatcttcc ttctggtgtg tcatgctcca aatacatccc caataaatcc cgtgccactt
     5821 ctggcgtcgt tttattccaa aaaaattcat ctatcttttg tttatccgtc atttttctca
     5881 cctactttat catacacaat attagttgga taagctatga aaacaaaaca acagaaggct
     5941 gttctttcta ggcatcttgc taaatatgat ataattaagg gacgtaaaaa caagaaggag
     6001 gaaaatccta tgcaacagcc attggcttat cgcatgagac ctcgaacgat cgatgaagtc
     6061 gttggtcagc agcatctagt cggggaagga aaaatcatac gccgcatggt cgatgctcgc
     6121 atgctctctt ccatgattct atatggacct cctggtactg gaaaaacgag tatcgcaagt
     6181 gcgatcgccg gctctactaa ttatgcattt cgcatgctaa acgccgctac agattcaaaa
     6241 aaggatctac aagttgtagc ggaagaagca aaaatgagtg gtacagtgat cctcttattg
     6301 gatgaagtac accgattaga taaaacaaaa caggattttc tgcttcctca tttagaaagt
     6361 ggccgaatca tcttgatcgg agcgactact gagaatcctt atattaccat caatccagcg
     6421 atccgcagcc gtacacagat ctttgaagtc aaaccattaa cagaacaaga catccagttg
     6481 gcagtcgaac atgctttaag ggataaagaa cgcggccttg gacaacaagc aatccagtta
     6541 gacgaagatg ctcttcttca cctttcgcgt gcaacgaacg gcgatttgcg gagtgcattg
     6601 aatgggctgg aactggctac actatcgact ccttcagata aagaaggaag gattcatctt
     6661 acactgtcga tcattgaaga atgtgtccaa agaaaagctc ttacccatga caaaaatgga
     6721 gatgctcatt atgacgttat ttcggctttt caaaaatcga ttcgtggaag tgatgtggat
     6781 gctgcacttc attatctcgc ccgtttagtt gaggcaggtg atctggcaag tatttgccgg
     6841 cgtctgatgg ttatcggtta tgaagatata ggtttaggta atcctgctgc tgcggctcgt
     6901 acagtgaacg ctgtattagc tgctgaaaaa ttgggattgc ctgaggcacg tatcccacta
     6961 gcagacgtcg tcgtcgatct ttgcttgtcg ccaaaatcca attctgccta catggcatta
     7021 gatgcagcac ttgctgatat tcgagagggc aaagctggag atgttcctga tcatttgaga
     7081 gatagccatt acaaaggtgc gaaatcactc aaccgtggcg tgggttatca gtatcctcat
     7141 cattttgatc aggcttgggt caatcagcaa tatttaccag ataaactgaa gaatgctcag
     7201 tattatcagc caaaagatac agggaaatat gaacaggctc tgggtcaaca gtattaccgt
     7261 ataaaagagt ggaaaaagca tcctccaaaa aactagacaa gaaaacggtt ctgtttctgc
     7321 gagctctctc attgctcttt ttaaattcat atgctactat agaggtggga aatctgtgat
     7381 gttcgaattg tcctctttgt gtgttgaccg aacattttta ttgatatctt gggacccgtc
     7441 ttgtgtcact gctggtaaca tgccttatca tttggtagtt cgaagctacg accatgggac
     7501 ccacctgctt gaattcgcgg gatcaatact acaggacaaa caacggcatt aacggagatt
     7561 accatctttt aaagaataaa gcaatttgct cttggcaaat tgctttttct atttgttgct
     7621 tattttggag gatttgttca tgattaggat tttaatgatt atcgccactt tagtattatt
     7681 attcgttagc tattatttat ttaataaaca ggatatcttt tttgtattga tcaacaaaaa
     7741 tgataaaaat caaggattcc tacaatttta cggagctgca tatgctgttt taggcgtaat
     7801 gggtatttta acagcttttt tcaatcaacg ctttattgcc ttggtctttt tactgatcgt
     7861 aattattgtt tctgccacct tcagtatccg atttgcaaaa aaaattgctg aaccaaagca
     7921 gtaaataatt ctctttttcc ctaaagtgtt ctacaataaa agagtacaat acgattgact
     7981 tgaggagtga atgcttatgt tacaacaata caaaaaaatc atggttgccg tagatggttc
     8041 tgatgaagct gaattagctt ttaaaaaggc ggtcaatgta gctatccgaa ataacggaga
     8101 acttctatta gcacatgtca ttgacacacg ttctttccaa accgtttcat cctttgatgg
     8161 aatgcttgca gaacaagcta ctgaaatggc aaagcaaaca ttggcagatt atgaaagcaa
     8221 tgcgaagaaa gctggcttga ataatgtaac ctctgtcgta gaatatggct caccaaaaca
     8281 aattattgcc agagaaatcc cagaagataa tcaagtagat ttgattatgt taggagccac
     8341 aggattgaat gctgtagaac gtttatttat tggttctgtt tctgaatatg ttattagaaa
     8401 cgcagcttgc gatgtgcttg ttgtccgaac tgatttagaa aaccaattgc cagctaaaga
     8461 ccacaccaaa gcttaacacg tagacgacct gccaactatc ggcaggtttt tttgcacaaa
     8521 aaaccgtgat aaaaacaaaa gacgcggcaa gcactcatcg gtctttataa tgtttaaaat
     8581 aaagggaaaa cagtcaatga gttcaagtaa aataaaaaaa agtgaacacc agggcgatta
     8641 gtcgccctgg tgtggtttaa aacgacctaa gcatgccaaa gcttattggt tcgtcaatgt
     8701 ggaagtttcc gatgaatctg atacgaaaaa atatgaagta tcgatggcgg ataccgattt
     8761 tatcgccaaa ctaaaatttt atttcgggtt cgggtaccgc gagaaggcaa gtgctacatt
     8821 cggtaaatca ttcaagaatt gatgaaaggc taccgcttgc cattgcaacc acaacgatac
     8881 tcctgaacgc gatgttggtg agatttccaa agagaagagc tgtttaatgc aacggtatta
     8941 agcaaatggc aaaaggaagt catgtagaca aagtttttta tcaaacacca tatgatttcg
     9001 cctgaacgct ggtttagctc agaatacaat gatgtggtcc acgagacatt accctcagtc
     9061 agataaaaaa agctctctga cagaattaca catcgataaa tattaagtaa atgtcagcgc
     9121 tagttgaact ttctgacact ttcaggtaca ctggaaaaga caactacacg aaggagggaa
     9181 aatactatga tgcgcatgac taaaataaca agtaatgaaa attaccaacg ataccatatc
     9241 gcatttagcc atgcaattgg ttttacagtt gctgttgacg ggacaagttt gtccaaaaca
     9301 gttgaattca cgtaaaatca atggccatct caaggtattt ttctcatgat aatctgagtt
     9361 taaataacac aggccatgga taaccatggc ctttttgagt ggtctcaagg agagagatca
     9421 tgatcataca agcaatttct attaaaaaaa caatcggcgg gaatccatta tttgaggatt
     9481 tatcctgtca aattgaagct ggtgaaaaaa ttgtcttagt cggtatcaac ggaactggca
     9541 aatcaacctt tttacaaatc ttgaccggca gagaaggtgt cgacagcggt gttatcagtc
     9601 gcaaaaaagg cttaaagatc gggacggtcg aacaagaact tacagtttcc gaagcgaccg
     9661 tcaaccacta cttgctccat tctgccgctg agatacagga cctcaaacag caaatgagtc
     9721 gctacgaagc attgatgaca gaaccagata caaacttaga gaagtgtctg gcccgttacg
     9781 gtgagctgca gcatcgcttt gaagaactag gcggttacgt attagaagac cggattgcag
     9841 cgatcttgca aggcttaggg attccacacc gtcaggaagc acggttaaca gaacttagcg
     9901 gaggcgaacg agttaaagtc gctttagcca aaattttagc agccgatgcc gatttacttt
     9961 tactcgatga accgaccaat catttagatc ttaacagtat tcgctggttg gaaaactatt
    10021 tacagaatac aaaaaaatcg gttctggtcg tttctcatga tcggcaattt ctcgatcaag
    10081 tcaccacaaa aacatgggaa ctggaagaag gtgcattgat tgagtatccc ggaaactaca
    10141 gccgttttcg agtcttgaaa gaagctcgct tggctgagct gacaaaaaat tacgaactgc
    10201 agcaaaaaga agtccagcgg ttgaaagtca tgattcgtcg ttttcgtcag tgggcacatg
    10261 aaggagacaa tgagtccttc ttcaaaaaag ccaaagaact ggaacgccgc ctggcgaaac
    10321 tgacactcgt caaaccgcca cctcccccga aaaatcggct gcaaagcttg tctaatggtg
    10381 gaaaatctgg caaagaagtt ttcatcattc aaaacttgca ccagcagtac gctgatcaag
    10441 tactattcaa agacagcagt tttgcagtct atcgtggaga tcatttagcc atcatcggcg
    10501 acaatggtgc aggaaaatca accttactca aattattact cggtgaggaa aagccttacg
    10561 gtggcaccat caaattaggt agtagcttgc aaatcggtta tttgccgcag cagctgcagt
    10621 ttgccgatcc cgatagccgt ttgctggctt acgccatcac attgactgga aacgaagaag
    10681 tcgctcgacg gcaattagcc aaaagtggct tttatcaaag cgatgtgggt aaacggatca
    10741 aagatttatc cggtggtgaa aaaatccgtt tggcgttgct gaaactcttt ttagaaaaaa
    10801 tcaacgtcct gattttggat gaaccaacca atcacctcga cagctatgct cgagaagaaa
    10861 ttgaagaaat gctgcaagac tatcaaggaa cgctcctagc agtcagccat gaccgctact
    10921 ttttgcagca acacttccaa gaagcactcg ttattgatca ggagcagatc agccgccagc
    10981 cgctcggcca actgattgac taggacgttt tttcgcaaca aaaaacttga ttttcatacc
    11041 tgtgctttca cgattcaggc aaagtacagg tggaaaatca agtttttatt attcgatcaa
    11101 tgaatgtttc ggatcggttt tctagctaaa cataaagcga agaataaagt cgacagccca
    11161 cgaggctgat ctaaaaatgt tttttctttt tacatatgta atgtccctta taaagatgaa
    11221 tgaaaataaa atattgggat tttcctgaaa atcaataaat aaaatgccag tcttttccct
    11281 tttgaccact tttccaagca aaattcccta agaagatcct gtaaaaaaga agggaaattg
    11341 aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa cacatgcaag aattccctct
    11401 tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca gacgctccac
    11461 atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta cttttgattc
    11521 ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat cgatttcaca
    11581 accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat tctcgccgat
    11641 accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac cgccgatgta
    11701 ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt tttcatagtt
    11761 cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac cagatttctt
    11821 gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta aatatgggag
    11881 aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta atggtgtgaa
    11941 tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata caccatttcc
    12001 taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt ttgccgcacg
    12061 ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt tgaaatcttc
    12121 ataataagac accggtaagc tatataaata attttgcttt ggcattgttg agtggaaaga
    12181 tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg ctttgatccc
    12241 cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa tgcggtccat
    12301 tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa caacacggtg
    12361 ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca actgatctaa
    12421 cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga atttttgatt
    12481 atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga cgatcccttt
    12541 cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg aactcccagc
    12601 attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt acagagcaga
    12661 tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt cttttaatga
    12721 agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt ttttctgtaa
    12781 gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca attgaatcat
    12841 tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt gatccgtttc
    12901 taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat atttcattga
    12961 ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg ttaaaaactc
    13021 ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg caaaatcgac
    13081 aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat ttgcttgtgt
    13141 caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca caccaattcc
    13201 ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca agttgcccat
    13261 tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata gttgttcaat
    13321 aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg cctgcaaatg
    13381 ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt cttctggctc
    13441 gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg tctcttttgt
    13501 cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg cattttcgac
    13561 ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag cttccaggtt
    13621 ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt ctagatatat
    13681 tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt caatcgtttt
    13741 cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg ttataacaga
    13801 aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat cattttttac
    13861 ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata gggactgttg
    13921 tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg aactttcggc
    13981 aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat aataattttt
    14041 gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca tcaataaaaa
    14101 cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat aacattttct
    14161 actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag tgtacataaa
    14221 aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt aacataggtt
    14281 gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg atgatatact
    14341 gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg tgactgctct
    14401 tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct ttacgctgca
    14461 aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc aatagactgc
    14521 tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt acttgttttt
    14581 tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac tccattatta
    14641 tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct tttgtgatac
    14701 atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc ctccccactt
    14761 ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac acagacagaa
    14821 gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt caattctttt
    14881 tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt tttcttccca
    14941 ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa aagtaataga
    15001 agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat ttattttatc
    15061 tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa agcctgtttg
    15121 gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa attggccgaa
    15181 aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg gaaaagaaag
    15241 gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc ctgatgctaa
    15301 aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata tagccttcat
    15361 tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag agctcttttt
    15421 gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga ttcctataac
    15481 gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag acgattagca
    15541 tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg gcaaaacctc
    15601 ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa tcaaagccgc
    15661 tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac gtttgaaaag
    15721 ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc cctttgcctc
    15781 tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct ggaactgctc
    15841 agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat gctgaatcag
    15901 actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga aaccttcatg
    15961 aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat atatcggtcc
    16021 aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact tcatcagacg
    16081 ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa cataccaatg
    16141 aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg aaccaataag
    16201 ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg tgagcaatag
    16261 catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa gccgaaactg
    16321 tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta actcgatctg
    16381 taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg cttgcccttc
    16441 ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga tcgtgagtaa
    16501 taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca tggtttgtgc
    16561 aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc caaatatttt
    16621 cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc tccataaatt
    16681 gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct tgataaatga
    16741 caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg attctgacca
    16801 atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct gttagtgctg
    16861 ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt agcaagtctg
    16921 gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt ttcggattga
    16981 tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata gtaatcactt
    17041 gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat ccgactggtc
    17101 cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca aaatctcgag
    17161 aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc actaaactct
    17221 ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt ttttctaaag
    17281 aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc tgtcccaaat
    17341 acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc cagaatgttc
    17401 gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat atatatacgt
    17461 cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt tttatttcca
    17521 ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca aataaaatga
    17581 atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa atttatcttg
    17641 ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag taaaaaaaag
    17701 cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa agaaatcatg
    17761 gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata cgaaggaaaa
    17821 agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg ccatgtgcat
    17881 atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct tcatggagtt
    17941 actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac aggtcttgag
    18001 tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct accttcttgt
    18061 gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa attagcccct
    18121 tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca agcagtagca
    18181 aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa gaaaaaaatt
    18241 gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc agcaggtatt
    18301 cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga tcttggtatg
    18361 tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact accggcgatc
    18421 acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat caatgattta
    18481 gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg aatcgatcca
    18541 ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt gaaaacacta
    18601 ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct taccaagctg
    18661 ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca actccaagac
    18721 catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact aaacgctcaa
    18781 ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca tgtgatcgaa
    18841 attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg acaaggcgaa
    18901 cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga gcgtcaccat
    18961 caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca aggagcgata
    19021 gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa cgacgaggat
    19081 atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt tgtcaacaat
    19141 cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca ggaacctttt
    19201 tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact aggagcagct
    19261 aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat acccgaattg
    19321 aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc accttgtgga
    19381 aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac attacatctg
    19441 taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa gcagaaagtt
    19501 agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact cgataaatac
    19561 gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct tcaaattgac
    19621 tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa aaacttgcca
    19681 gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca tttggacgaa
    19741 gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt tcatgacgtt
    19801 tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg tcagcgaaat
    19861 actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc ggttgttcga
    19921 tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca atataattga
    19981 tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata ccaatctcaa
    20041 tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac aatgttggat
    20101 cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga cgatttcgga
    20161 cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca aatctccgca
    20221 gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt tcttcgttca
    20281 tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa ttcatggccg
    20341 cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa ctgatcagcc
    20401 actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata tccaccgcag
    20461 ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta catactaacc
    20521 aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct attgacggga
    20581 tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg agttcttccg
    20641 gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga ccaccgatac
    20701 gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca tcaatcacgt
    20761 cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac tcttgtgctg
    20821 tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta attccttccc
    20881 ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg tcctcatcac
    20941 gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc cactcttggt
    21001 ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg cagtacttat
    21061 tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc atttaattgc
    21121 cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt actgtcaacg
    21181 ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg acatcttctt
    21241 caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat aagccgctag
    21301 cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa aaataaccga
    21361 cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct gattcttatg
    21421 gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag gtaacagttg
    21481 gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa agtcgactac
    21541 tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac taaaatcaga
    21601 acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt ctcttttaat
    21661 atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa tttcaggaac
    21721 atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt ctacatgcac
    21781 cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt aatatagact
    21841 tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg atagaaacaa
    21901 aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt gttgatctag
    21961 tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc ataatatgat
    22021 cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta atggcatggc
    22081 caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc gaaaagctcg
    22141 ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat ctgctgcctc
    22201 tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag agattatgag
    22261 ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat ataatcccac
    22321 accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat aacgcatata
    22381 tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac tcatgtgcct
    22441 gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc tgaaattctt
    22501 ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag accacaaaac
    22561 gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata ccggctcctt
    22621 cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga tccaagccta
    22681 aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat aagatcatca
    22741 ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga ttagataacc
    22801 gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt atatcgtagg
    22861 ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa gaaggagtct
    22921 ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag gatagatgat
    22981 tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg atggtcgtac
    23041 ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt acatcgaaac
    23101 tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg ataattaaac
    23161 tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa atgacggtat
    23221 tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa gtatttattc
    23281 tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat caatcaagaa
    23341 atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga catctctcaa
    23401 gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat ttttgaatat
    23461 gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca agcattggaa
    23521 aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga actagcctat
    23581 caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga ggatgatttt
    23641 tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa agctattcaa
    23701 gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag gactgttatt
    23761 tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg ggaatgaaac
    23821 aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg atttaaataa
    23881 cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg aatctgtgat
    23941 tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc aagccgaacc
    24001 acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc cagagtatcc
    24061 t
//
LOCUS       DASJST010000032        23363 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJST010000032 DASJST010000000
VERSION     DASJST010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.032"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..564
                     /locus_tag="U4B05_001007"
     CDS             <1..564
                     /locus_tag="U4B05_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2050159.1"
                     /translation="NYHVVDQETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHL
                     SMATAWEILERSNYDKKILFISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVV
                     FDDIGAELGSGVSNSRQFTNNTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRI
                     FKNSEGYALKFQQTADKRIKPVKGSIA"
     gene            561..920
                     /locus_tag="U4B05_001008"
     CDS             561..920
                     /locus_tag="U4B05_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN2050160.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            935..1096
                     /locus_tag="U4B05_001009"
     CDS             935..1096
                     /locus_tag="U4B05_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2050161.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            1093..1398
                     /locus_tag="U4B05_001010"
     CDS             1093..1398
                     /locus_tag="U4B05_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050162.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            1398..1715
                     /locus_tag="U4B05_001011"
     CDS             1398..1715
                     /locus_tag="U4B05_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEN2050163.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            1712..2098
                     /locus_tag="U4B05_001012"
     CDS             1712..2098
                     /locus_tag="U4B05_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050164.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            2095..2430
                     /locus_tag="U4B05_001013"
     CDS             2095..2430
                     /locus_tag="U4B05_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050165.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            2576..3145
                     /locus_tag="U4B05_001014"
     CDS             2576..3145
                     /locus_tag="U4B05_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN2050166.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            3142..3315
                     /locus_tag="U4B05_001015"
     CDS             3142..3315
                     /locus_tag="U4B05_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050167.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            3312..3617
                     /locus_tag="U4B05_001016"
     CDS             3312..3617
                     /locus_tag="U4B05_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050168.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            3641..3838
                     /locus_tag="U4B05_001017"
     CDS             3641..3838
                     /locus_tag="U4B05_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN2050169.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            3835..4041
                     /locus_tag="U4B05_001018"
     CDS             3835..4041
                     /locus_tag="U4B05_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050170.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            4041..4337
                     /locus_tag="U4B05_001019"
     CDS             4041..4337
                     /locus_tag="U4B05_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050171.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            4413..4826
                     /locus_tag="U4B05_001020"
     CDS             4413..4826
                     /locus_tag="U4B05_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN2050172.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5510..5677
                     /locus_tag="U4B05_001021"
     CDS             5510..5677
                     /locus_tag="U4B05_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050173.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5703..6047
                     /locus_tag="U4B05_001022"
     CDS             5703..6047
                     /locus_tag="U4B05_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2050174.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            6052..6333
                     /locus_tag="U4B05_001023"
     CDS             6052..6333
                     /locus_tag="U4B05_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050175.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6436..6750
                     /locus_tag="U4B05_001024"
     CDS             6436..6750
                     /locus_tag="U4B05_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN2050176.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6728..8422
                     /locus_tag="U4B05_001025"
     CDS             6728..8422
                     /locus_tag="U4B05_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2050177.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8442..9620
                     /locus_tag="U4B05_001026"
     CDS             8442..9620
                     /locus_tag="U4B05_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2050178.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9583..10269
                     /locus_tag="U4B05_001027"
     CDS             9583..10269
                     /locus_tag="U4B05_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN2050179.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10269..11429
                     /locus_tag="U4B05_001028"
     CDS             10269..11429
                     /locus_tag="U4B05_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2050180.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNDFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11439..12314
                     /locus_tag="U4B05_001029"
     CDS             11439..12314
                     /locus_tag="U4B05_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050181.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12311..12622
                     /locus_tag="U4B05_001030"
     CDS             12311..12622
                     /locus_tag="U4B05_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050182.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12612..12965
                     /locus_tag="U4B05_001031"
     CDS             12612..12965
                     /locus_tag="U4B05_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2050183.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12955..13356
                     /locus_tag="U4B05_001032"
     CDS             12955..13356
                     /locus_tag="U4B05_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050184.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13349..13753
                     /locus_tag="U4B05_001033"
     CDS             13349..13753
                     /locus_tag="U4B05_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050185.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13765..14373
                     /locus_tag="U4B05_001034"
     CDS             13765..14373
                     /locus_tag="U4B05_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2050186.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14393..14755
                     /locus_tag="U4B05_001035"
     CDS             14393..14755
                     /locus_tag="U4B05_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050187.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14758..14940
                     /locus_tag="U4B05_001036"
     CDS             14758..14940
                     /locus_tag="U4B05_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050188.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14958..18290
                     /locus_tag="U4B05_001037"
     CDS             14958..18290
                     /locus_tag="U4B05_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2050189.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            18341..19078
                     /locus_tag="U4B05_001038"
     CDS             18341..19078
                     /locus_tag="U4B05_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2050190.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19088..21379
                     /locus_tag="U4B05_001039"
     CDS             19088..21379
                     /locus_tag="U4B05_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2050191.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            21403..>23363
                     /locus_tag="U4B05_001040"
     CDS             21403..>23363
                     /locus_tag="U4B05_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050192.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGS"
BASE COUNT         8341 a         3476 c         5040 g         6506 t
ORIGIN      
        1 aattatcacg tagtggatca agaaacaaaa atcgctttag aaaaagcaaa aagctatacg
       61 aatgaggttc tgctgaacca tcctgcacat ttcattctat cagggaaatc aggaagcggg
      121 aaaagccact tatcaatggc tacagcttgg gaaatacttg agcgctcaaa ttatgacaag
      181 aaaatacttt ttataagcta tcaagaatta ttagagcaaa taaagttttc ttataacaat
      241 gctgaactga gaaaagaaat tgaaggatcg cttatagccg atattaaaac aactgatttg
      301 gtggtttttg acgatattgg agctgaatta ggtagtgggg tatcaaatag taggcagttt
      361 acaaacaaca cgttaaacac gctcttagaa gcaagacaga acaaggcaac gatcatcaca
      421 acaaacttat ctggtcctga actaagagaa gcctacggtg aaagaattgt ttctaggata
      481 tttaagaatt cagaaggtta tgcgctgaaa ttccagcaaa cagcagacaa gcgcataaaa
      541 ccagtgaaag gtagtatcgc atgaataaat accgtaataa aaaaactgtt catcgaggta
      601 tcaagtttga ttctatcgca gaagcagagt attacgatct agccttgtgg caagctgaag
      661 cgaatggctg gaaagtaaaa cttcaggaaa gatttgagct gatgccgaaa tttgaactag
      721 acggaaagaa gtatcgcaag atcgagtata ttcccgactt cacattttat aaaaacggca
      781 aacttgtcaa agtcgtagat gttaaaggaa tgcagacaaa agactttaag atcaaggcaa
      841 agctgttttg tcatcaatat caagtgccgt tgatattagc taaaaaatat cggaatacgt
      901 tcaaggaaga gcgtttttaa agaggtggtc tatcatgaca acagaagaag tgattcaaat
      961 gcgaattcga agcattcaac gtgagattga cgatctggaa cgaacaaagg cagtgatggt
     1021 caatgaaacg gcgagaaagg caatcgattt gcacatagag aacttaagaa gggaaattcg
     1081 tagattggag gaatgagcgt ggataagaaa gcagcaatga aacgaatcat cgaactgaca
     1141 cattctgaga attggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa
     1201 tcaatgtgga ctgaaaaaac cagacggaga atgccgagaa aaattgcaat ctggcacggt
     1261 gaccgaattc tagtaacagg taccgctgaa cagttatctg aaattactgg tctgagcaaa
     1321 aacatcatct gggatagagc gaagaatatg gatattgatt ctaaaggaag acagtttagg
     1381 tatgtggagg agaaataatg aatctcatta cacaatacag tgatatcatc ctcaagaaaa
     1441 tcatgatgaa gattcagaaa gacaaaaaat caaaagaacg agcggaatta gttaagttgg
     1501 aaatggctga aacaggggca ggagtgcgaa gtagcaggca ttggaaggca gcagcaaaca
     1561 ttgaatttta ttacaacgaa attcaaaaag ggttcgatca aatgcgtgag ctggatcggc
     1621 aaacaaattg gagcaagaaa cttcatcaag atcgtttcaa atttgtagag aaatataaag
     1681 agatattaga cgaatacatg gagggcagca aatgataccg agatttcgat cgtggtacac
     1741 accatttaaa ggtaaaacaa ttggacaaga aatgaaatat gggcaagcag gaaggttgat
     1801 cactcatgct gaaatggctc cagataaata tgtgcttatg caatccacag gactgaaaga
     1861 taagaatggc gtagaaatat ttgaagggga tgtagttctg gttaatgtaa gtaatggttt
     1921 tgatcattta gttaatgaaa aaacggttgt acaagaatcc gagtttcatt ccggattagt
     1981 ttgtaaatca ttaattagtg gaatggaata tagaatattc agccaccaaa agatgggata
     2041 cgaatatgaa gtcaccggaa atatacacga gaatagcgag ttattggagg tagagtgatt
     2101 atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt cccagcattg
     2161 ctaaattgtg gaattaaaag taagaataga atcattttgt tacggatatc ggctgattta
     2221 gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac acgttatcag
     2281 tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc tgacatacat
     2341 ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc cgatgatttt
     2401 ccaaatttat ggttgaagga ggaacagtaa tgaaagactg gttagaagaa gcaagcgcct
     2461 tagttggctg tttcataaac tgtgcgttta tatctgtaat tattgcactg gtgggatttg
     2521 tatcattgaa agtgattata ttgctttggc agttaatttt ttaggaggaa cagcgatgaa
     2581 taaacaggaa gcaatcaaaa agttagaaag tattaaagtg ataggaaatg atgcactagc
     2641 tgcttgctat aacgagagta taaatgcagg tattacgtta atgaaaaaaa tagacgaacc
     2701 gaagaagcta gttattccac aactcgtggc cggttggctt gagaaatcta cggacccttt
     2761 tacgaaagct gaaaaaatag cgtatttaat caaatctaaa gatggtgatt catattattt
     2821 ctgtgattgg tttgtacgag atggcatagt gacgcaagaa caaggagaag aattactcgc
     2881 ttgggcaacg agacaatcat atgaaacact attgagcttg tacaacggct acgaggtcga
     2941 gaaagagccg ttgtacagtg taataattgc tggcgagtac cttgtcaagg agatcagttg
     3001 cagtaatgaa gttaaactag tatcaacagc ttacctgcca caataccttg cacaccacta
     3061 ccaactgacc gaaaaacaga tcaaagcaat tgatgaaaga tactggccat ttgctgtgcc
     3121 agtggaagag gtggcggaag gatgagtaga cagataatgc tccatatacc agacggaaca
     3181 aaggcaatga cgattaccat tgttgctgca caaaaagacg gtgggttggc gttgaaaaca
     3241 aaaagcattg ataccaaaca gattttagaa ggcagagatg tcgagataga gatcgatgag
     3301 gaggaagcgg aatgaatgtt caagtttatt taaaaagaga tttggccagg tttcctcgaa
     3361 gctgggatac aaattcccac ccatccgagg cagaagaaga gggctatcta atgtctgcta
     3421 acatgataga aattacaaga gatgatgcgg atgagtttga taaaaaaaga tcatggtact
     3481 cggttccgaa tccaatgtat acggctgtaa tagaagatta tcatatatcc gacagattta
     3541 ttctgattga tctagaaagg cctaagaaac gaattaaaca gtatagacgt tgtggatttt
     3601 caacaaagaa atggtgataa caaaacagag gtgaagaaaa atgaacaaca gacaccgcag
     3661 agtagcaaaa ctaagaaatc aagaactgaa tgtagcgaaa gtaaagttcg aacgggagta
     3721 tggtgtttct gcagaagaaa catataaagt ggcaagtcag tgtgttgctg atgcaagtga
     3781 tgctattcgt aagtttggaa tttcgatact agatattaaa tgggaggata cagaatgaag
     3841 atttatgtag taaagtttgg aaatcaattt tacagaagcg atgaacgttc tataggggct
     3901 aacacattat ccattgtaga catactccaa agcgcaagat ggtttgataa tctcgaagaa
     3961 gctaaccaag tttcacgacg acttggtgga ttaacgcaag tatatgaact ggtcactgtc
     4021 gatcatgagg gggtagagta atgaaactaa aagacggatt ttacgctagt agtcatggta
     4081 tcggcggttt gatgctagat atgccgacaa agaaccctaa aacacgtgag aaaccaaaat
     4141 tcaaagtcgg tgacatggtt cgctgtgaag cagaaggatt tatctatcca tttcgtggat
     4201 atgtagaaaa ggtattgtcg aactcagcaa tcattcgtat tgaaaacacg atggaatgtg
     4261 acaagtggtt agcgaaaagc aaagagaatt tagctgtagc gagattagtt gacatggaag
     4321 taatcgagag caaataaaaa agccggatcg ctccgactaa cataataaaa cagacaagtt
     4381 tattatatca cataaaggag cggtttgact tgatgcaatt gttacgagag gtagatttca
     4441 aacagacaag atgtaatgcg agagatgtgc tgaagaactt tcggcgtttg gagcggatgg
     4501 caggtcgctc tttgatagat attaagtctc caattattac ggatatgccg aaggcgccga
     4561 agcacggcaa taaggcagag gacgcgatca ttcagatgat ggatatagaa gcagagagag
     4621 atgcgatttt agcggctttg atggctctta gtctgattag ccgtcagata ctctactaca
     4681 gcttttgtgt gccagatagc ttctcaaact acagaattag ccgtgaagtg ggttattcag
     4741 aaagaagtat acaacggatg aagtcggaag ctctaataga gtttgcagaa gcatataaac
     4801 acggaagaat aattgcttat aaataatttg gcggtttttt ggcggaatga tggcggtttt
     4861 tagctattta ccagtgatat tatggtagtg tcgaaagatt agtgataggt ctgagacaaa
     4921 ataataataa aaggaacatc gttttattat tgtttcacaa ttaagcttcg atagacagca
     4981 acggaaatat taagaataag gatgtgaatt tcaactcctt ctaaattgtt cttattatct
     5041 atcatccgtt gctgtctatt gtttttttaa ttattcacac gataactaaa ggtgagtgaa
     5101 gagaaatgat tccattaata atttcaattt ttgcgctctg tcttaatgtc tatatgattg
     5161 gatttaaaaa tgggcaaaat aaaaaatagt agcagccaag aataatttta tagtgtcact
     5221 gtggcggaaa gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt
     5281 tttatgattt gaccatgcaa tgttcgattc attgccagtg actttggcaa ccgagggttg
     5341 gaaatgggcg ctcaaagtac acgagcaagg cgaggtcgat agtaatcgat ggaatcggtg
     5401 taggttgctt gatagagcta taactgcatc tcgtttatga ggtgtagttt ttacagatta
     5461 gatcactctt tgagtggtct ttttatttta cacaaaggag gtaacaagaa tgtatagacc
     5521 acaatactta gaacagaagt atgaagtaat cactgtgcaa aacggtaacg gtgagatagt
     5581 acgaaagtat agaagaccaa taaagagcga tacatacaaa cgaaaggaaa gcaatgaagt
     5641 tattccattg tatggcaaaa gaatagctaa gcattaaata agattgcgaa aggagacgga
     5701 atatgaccga ggaattctat agatggctat tacagttgat aagagaagat cgtttagtta
     5761 agttctatca gtctcctaaa tggcgcaggc ttagagagaa agcgatgaaa cgagatcact
     5821 atgaatgcca agagtgtaga agactaggta agtatcatag agtagaaaac gttcatcata
     5881 taaaggaagt caaggataga cctgacttag ctttagattt agataatctt atttgtttat
     5941 gtgttgaaca tcataatgaa gttcatggca gatatcttac agcattagat aaacaagaga
     6001 agaagataga aagctttgct aacttcgatg caagtgaaag gtggtaagtg catgatcatc
     6061 aatgataatg gcagagagta tgatacagaa aagattgaag agtattcatc ttatactcag
     6121 ggattaatta aacgtttgat atacgttcgc tatgtaggta ttagggatct gttatcagat
     6181 aactgttgta gtaaatacaa agtgaatcaa gtaagagaag cgttgaataa agataataac
     6241 gtcgaaagaa taaaaaatgt ttttggatat agtattgaag agattaatta ttacattgac
     6301 ttcgctgaag ctttcattcc gatggtgaga taaccccccc ttaaaataaa tcgcaaattt
     6361 tttgggggtg atgaaacgga gggggctgtc aggaaaagag attttttcga actttatcat
     6421 gaaaggaggg ctaaaatgtt taaaaacgaa ttgtctcaaa atcgctacag agaaaaatta
     6481 cgccgctctt taataagcca attggaaagt cagaaaacaa atattgagcc attcttagat
     6541 aatgttgatc gttatatcag tttatgggaa acggcgatat cactggaaga agatatatcc
     6601 gagaacggca ttagactgga gaatggtaaa aagaatgaat cagtagcgtt gcttgtttct
     6661 gtcaacaaac aaatgggatt gatgttggat aaacttgcca ttactcctga attggtaggt
     6721 gaagcaaatg aatcaattcc tgagttataa gcatattgaa aattggttca aagctataga
     6781 agaaggcact atcaaggtat gcaaagagca attattgcta aaaaattatc tagaagaaag
     6841 agtctttact agagaagata tttacttcga taagcagatg gtagaggatt caatcaatat
     6901 accagcacaa tactttccat tcgaattaat tccgtgggaa aaatttctac aatgttttat
     6961 ttatggtgtt cgatggaaaa aagataaaac actagtgttc aatagatatc tttcattaat
     7021 gggacgtggt aatggtaaaa ctggttttgc ttcttggaac aacttctttc tactaaccgc
     7081 taaacacggt attaaaaatt atgatattga tatctatgcc aataatgaaa gccaagcaaa
     7141 gactagtttt gatgatgtat ttaaagtaat taaagatcat cctgatttag ataaaaaagt
     7201 atttaaagct acgaaggaag ttattcaaaa tatcgctaca aacagcaaac ttcgttataa
     7261 cacggcaaat gctagaacaa aagatgggaa gcgaccaggt gcaaaccgct ttgatgaaat
     7321 tcacgaaaat gaagattatt caatgataaa tgtggctact tctggtggtg gtaaaattcg
     7381 agattataga gaattttatg atacaactaa tggtcatgtt cgtggtggtc cgcttgatga
     7441 cattatagaa gaatcaaaaa tgattctttc tggagaactt ggaattgaca aggatggagc
     7501 agaattttct agtttgtttc catttatttg tcgcttggat aacgataatg aagttgatga
     7561 tcccgacatg tgggaaaaag cttgtccaac tattaattac aatgcagatt taaaacggaa
     7621 aatgtttcaa gaatactctc aaatgcaacg taatgctggt ttaagactta cgttcatgac
     7681 caaacgaatg aacagaccta tggaagatac acgatttgct gttgcttcat atgatgatgt
     7741 tctgcatacg aaagaaaaag aatttcctga aaaaatggat gaagtgatag gaacagtcga
     7801 ttttgctgat agacgagatt ttgccagcgt tgggttgcta ggaaaatacg ataaagatgt
     7861 ttattttaca caacatactt ttatccacga atcagccctt cgattacaaa acatcaaacg
     7921 agaggttata gatatttcta tagatcaagg aaaatcacag atcgttcatg gaaaaaatat
     7981 agaagctgat tatattgtag gttggtttct tgaaatgagt aataaatatt atattaaaaa
     8041 aatcgctatg gatatgtacc gtgcaaaaat attgaagccc gctttagaag aagcaggttt
     8101 tactgtggaa attgttcgaa gcggatctgt tacacatggt atgttaaaag atctggttga
     8161 tgaccttttt attaatcaac gtttattttt tggtgacgat gcgattatgc gttggtattg
     8221 catgaatgta tatgaagagc atatttctaa tggaaatata cgctatgaaa aaatagaacc
     8281 tgaaactaga aaaacggatg gctttttttc attccttcat ggtttgaatt ttttagatga
     8341 tatttatgat tctgctcctg taacagtcac aaatagctca gtagaaaata caggaactgg
     8401 atttactcct ctagtattct aacttgaaag gaggtgagaa agtggggatt tttcaaaagg
     8461 cggtaggata cttcacaaaa aaagcaacgg ttcctttaga agaatacttt tgtaaattgc
     8521 aagttgattt tgtgtatcga aaatttgcga ttgaaacttg tattgatttg attgcaaatg
     8581 cgatgagtaa agcggaattc aagtcatatg aagatggaaa aaataaaaag aatgatcttt
     8641 actataggct gaatgtagct cctaataaga aaaataatgc aacagaattt agaaaaaaac
     8701 tgatcaggag attaatattc tacaatgaag tattgatcgt ttctccgtct aataattcta
     8761 gcgaaatatt tattgcggat agttgggatg tcacagaata tgcattgaaa gatgatgtgt
     8821 tttctcaagt gcaaattaac aacatagtcc ttgatagaga atttctagaa agtgatgtta
     8881 tctatataaa atacgcagat caacaaatta ggcaactagt cgatgcgtat tatcaagcgt
     8941 atgggaaact catttctagt gccatgaatg tttacaagcg ctctaacgct cgtagatacg
     9001 tactgaaagg gaatttattc cgaccgcaag acaatacaac acaagatcaa atcaataaaa
     9061 tgatgacatc acaatttaag gcttttatgg aagctgataa tgcaggtgcg gtatttcaat
     9121 tacaaaatga gtacacatta gaagatttca gcggaaactt ccaaagcaat tcaagagata
     9181 taaaaaactt aatagacgac atctttgaga tgacagcagc agcgtttcac gttccgaaaa
     9241 acctactaaa gggagacatg agtgggttat cggatcaagt ggacgctttt ttaatgttcg
     9301 aaatcatacc gattgctgaa cttattcagg atgcgtttaa cgctagtctc tatgaagtag
     9361 aagaatactt gtcagggaat tttgtacgtg tggatacaac tatgatcaag attactagct
     9421 tcaaagattt ggttgacgct attgatgtag gcattagaaa tggagtattt acaatcaacg
     9481 aaggaagaga acgcgttgga aatgatcgct ctgataaggc gatggcagat gaaatattta
     9541 taactaaaaa caatcaacaa gtatcgaaag gaggtgaggc gaatgacgac aatgaaaaca
     9601 tttctagcag taaagaatga aggcgcagta ccgcaaattt ttattcaggg atttattggt
     9661 tctagttggt tctttgaagg gaatactgac aagggaatca aaaatatttt ggatagtcta
     9721 ggtgatcaag aagaaattga agtagtaatt aattcaaacg gtggagacgt atttcaaggg
     9781 attgctattg ggaacttact taagtcaaat aaagcaaaag ttaacgttgt gattaacggc
     9841 ttagccgcta gtgctgcttc aattatcgca atggctggcg atactataaa aatctacaac
     9901 aatgcacaat tgatgattca ccgcgcttcc acatacggag aaggaaatgt cgatgacttc
     9961 cgtacgattg ctgaccaact ggaatcaatt gataaatcgg taaaggcttc atataaaaca
    10021 cgattcaatg gcacagatga agcattgcaa gaacttcttg aaaaagaatc gtttatggat
    10081 gcagaaacag ctttgagtta tggattggtc gatgaaatta tcgatgcaga aaatagctca
    10141 ggtactgaag ctaaaaaaga acaaagcgtt gaagaaattt tgaatgacgt taaagaaaaa
    10201 agagcagaaa aaattgctgc atttacagca gcattaaata aaacatttgg acaaggagat
    10261 gtaaaataat gacagttaaa aatttaaaag gtgtaacagc tgcaagcgac caattgatga
    10321 aagcttttaa agatggtaac gaagaatctt ttagcgcagc tatggtaagc ttatctaagg
    10381 aaattcagga taaaatttta gaagaagcaa cagcaaaaaa tcaagatcaa ttagtattaa
    10441 tgaaccgtgg tcagcgtgta ttaactacac aagaaacaaa attctataac gaagtagtga
    10501 aaaacgaagg ttttgcaggg gtcgaagaat tagtgccagc tactgtattt gaacgtgtat
    10561 ttgaagattt agaacaatct catccactat tgcaaaaaat tacttttgtt aacacaactg
    10621 gtgtaacaga atggattgtg tcacgtggag tcaatccagc atggtggggt aaactttgcg
    10681 aagctgttaa aaaagtttta gataatgact ttgatgtaat taacatgaag cagttcaagc
    10741 tatcaggtta tattcctgta tgtaaggcaa tgcttgattt aggtccagta tggttagatc
    10801 gttatgtccg tactgtttta gtagaatcat tgagaattgc attagaacaa gcaattgttg
    10861 atggtactgg taaagatatg ccagtcggaa tgatgcgtga catgagcaaa caaactagcg
    10921 gagaatatgc tgaaaaaaaa gcagaaccta ttacagcttt agatgctgta actatgggcg
    10981 gtttgatggc gcgactatca aaattcaata tcgaaggtgt gaatgatccg atttatcgta
    11041 atgtgaatcc ttctgatgtg gtcctaattg tgaatccaac tgattactgg tctaaagtat
    11101 tcccagctaa gactgtacta actgctaatg gagaatacgt acaagtattg ccagtaccag
    11161 tttcagattt gcagtcaacg gctgtgccag aaggaaaagc agttattggg gtagcctcag
    11221 attacttcat gggtgtagga tctacactaa aaattgaagc ttcagatgaa taccattttg
    11281 ttgaagacga acgcatttat ctagctaaac aatatgcaaa cggtcaacct aaacgtaacg
    11341 atagtttcat tgtattagat attagcgctt tgggaactac tactacaact acaaaaccaa
    11401 caaccacaac aactacaaca caagcgtagg tgatcagaat gaagtatatt ctttgtcagc
    11461 cggcaatcaa tcggtttaaa tgggagcttg aagtttgttt aactaatctg aagaaactag
    11521 gaatcaaaga tatcgtattg cttttcagca gacacgatga tcagattcct attttttttg
    11581 agaaggaata tggtgttgaa gttcatgtgt acgatgatct gcgggacgac aaagagtata
    11641 ttccttcgat taaaccatat ttatggtgga aatatttaga agaagatcat tcgcgtgagg
    11701 acgaccgata tttctatatc gattcgaatg tcattttcaa taaaagaatt aatttgcgca
    11761 aattgccttc taaagatgat gtttggtatt gtagcgactg ctgtagttat ctaagtcttg
    11821 attatattag aagctgtgaa aacggagaaa atattctaaa agatatggca aacattgtaa
    11881 atgttacagt agaatctttg gaaactataa acactaattc aggaggcgca cagtgggtta
    11941 ttaaccgtcc taaagcgaat tattggaaaa aggtttatct ggattctaat cggctatatc
    12001 gctaccttag agggcaaaaa acaaatatac aaatctggac agccgagatg tgggcacagc
    12061 tttggaacat gatgtatttc aatattggtc ctaaagttca cgaggaatta gacttttgtt
    12121 ttgctactga tccaatagaa aaagttaaag aagtaaaaat cttacacaat gctggagtaa
    12181 caacaaacga tgaagattta tttttcaaag ggagatacgt gacttctacg ccttttgatg
    12241 aagatttatc atttgtaaac aagaaaaaat gctcttacgc atatgttaaa gcaattaagg
    12301 cggtggttag atgacgcctg aacaagtgac tgaagaattg ctaatagctg tgaaggataa
    12361 tatttacgtt acctggaacg aagaagatga gtcaattaaa aagatgatag ctaaaaatgc
    12421 tgtttatctt caaagtaaag tgagtacaac actttctttt tctcctgaaa gcttagaata
    12481 cggattgcta atcgaaagat gtagatacga ctggaatcgt gctttagatg agtttgaaca
    12541 aaatttcgct agtgagttat taggtttcat tcaacattat gcgctacaag aatatattgc
    12601 aggtgatgtg aatggcgaat aatcgtagac tcgaagaaac attcaacgat ggttggttaa
    12661 agattttgac gcaaactacc aaaagaaatg aactaggaaa aaagattggt gtagaagata
    12721 cagaaatcac ttctttaaaa tttagaaatc tttccatgag agatagtgat ataacagcta
    12781 tggatgcgat gggatcgaaa ttaactaaga aagtaaagac tccatttcat ccaatcgcca
    12841 agaaatttaa taaagatcaa tattttatcg taatcgatag tatgcgttac aacgttatct
    12901 atgccgatta cgataatttt tatatctatt tttatcttga aagtgtgggt gaatatggtg
    12961 attgataatt ctaaagaaaa agaacgttta aataagcaaa tttctgctat caaaacttcc
    13021 ttagaagagc attttggcct caaactcttt caagactccg taggcgagga tgagctacct
    13081 gatgatttta attacttcat tctcgaaaca ggagaaatag aaatgatcac tgagccaaaa
    13141 tatagcgtgg gtcaaaatct atatctaact ttctattcag aaaatagaga agatttaaca
    13201 ggagactcac tagatattat ttcattgatt caaaatcgtt cgattcgttt tcagagaatg
    13261 gatcccaatc atttaaaact agagaaccaa gatcgctata tcgatcaatt ggtatttacg
    13321 tttagacgat tattgaagag tgattgtcat ggctaaaaat agttgggagc taaaaataaa
    13381 tggacatgat gaacttcttg tgcggatgga acgctattca agcgagagcg aacgactgat
    13441 taacgaagca ttgaaatcaa aaggttcggc tattgcagtg gataggatta cagaaaaaat
    13501 tcctgtttct gaagcagatt taagaagagg gcaccaacac gcaaaaaata gtcgtccact
    13561 taagactcaa tacattaatt tgggtttcat cattagacct acaagaaaat ttgagtattt
    13621 aaaatatcct gatttgggga taggtacttc taaaagaaat cagccggacg aatttatgag
    13681 aagaggatta ggtcttgcac ttgatccaat tacagaactt ctgattcgtc aattcgataa
    13741 attaaataaa tagggggaac aacaatggct aaaacaaaaa ctgtagtaac aacgttcgat
    13801 aacataagta tcaaacgaat tgcttttaat tttaagaacg caggaaatgc aatcgtaacg
    13861 aattgtaatg gacaattaga tggcgaaaca gaaatgcaaa cggtggttaa aaaatgtgga
    13921 gcgacagaag taaaatcaaa atctaaacca atcaatatga cggtaacaat tactgcacat
    13981 gtaccgatgg aagtttatcg acgtttcaat gggttgaaac aagatggacg tattaaacca
    14041 ggcatttact cttacggtcc tgattccgta ggcgaagatt tctcacttgc tgcagagatc
    14101 gtggatgact tcgaagaaaa tagaaagtta gttggtatgt tagcatgcac ttcgaataca
    14161 ggattaacat tctctattga aaatggtgcg gatgaagtag ctgcgttaga actagaaaca
    14221 aaagttatgc aagatgaatt tggtaaattt tatcatgaag caattgttgc agaacttgaa
    14281 gaagacttaa cagatcaatg gatgacgaat ctatctgctg atgtgattaa aaaaacttca
    14341 gttgtgacaa ctacggccac tcaatcacag taaaaaaaac ggaggtagcg aaatgaacga
    14401 agattactca aaaattgaac taaacgatgg aacaattttg aatttagaac ctaaactgaa
    14461 tatcaagaaa ttattgatga tcaatagaga ttttaacaca gacgagtttg caaaaatgtc
    14521 gatgggaaaa ggctctatgg atattacagt tattcaaggt gcaaaagccg tatatgtagc
    14581 ttatcgtcaa gcgaacatgg tcgattacat ttcattcgat gaatttatag ataaatggga
    14641 ttttgatatg gaggttgcag tagctgtata cagtactatg atgttcaaac aagcacgtga
    14701 tgcctatcaa aaagaattcg aaaaagcaaa taaggaaaaa aagcttcaaa agtaaaaatg
    14761 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
    14821 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
    14881 aacaagagcg cttatgaagg atggaagaac tatgcaataa atcaatccca gaaaaactaa
    14941 aggaaaggag gtaaaaaatg gctaaaaaga gaacggaagc agaagtaact ttcatagcta
    15001 acgatgacgg attgaaatct acgttaaaag aaatcagtgc tgaattaact aaaaatagag
    15061 cagaattaaa actagaacaa gctcaattac aacagactgg ttctgaatca gacaagttag
    15121 gaagtaaatt atcttcttta gaaaagcagt atgaattaca aagtcaaaaa gttgaagtaa
    15181 ctagtcaacg tttagccaat gccaaaaaat attatggaga aaattccacc gaagttcaga
    15241 aacttgaaag agaactgatt aatcaacaaa cagctcagca aaggttatct aatgatatag
    15301 ataaaacgag taaggcattg gctcaatcta aaggtgaatt taaaacgtat gagtcaacta
    15361 tgaaagactt agataatgag caacaacatc taaagtcaag cgcatcatta gtagaatccg
    15421 aatataaaaa atggcaagca actgctggcc agtctgctac agaatctgaa aaattagcaa
    15481 aagcacaaga atatgtcggc aaacagagtg atattgctga acaaaaaatc gaagtgctaa
    15541 aaaaacaatt agatgccaca caaaaagaat ttggagaaac atctaccgaa gctttagaaa
    15601 tgaaaactaa gcttaatgat gcggaaagag aattcgaaga attaagtaat gctgctaaaa
    15661 acgttgatac ctccacagta gatgatatcg gcaaaaaact tgatatgggt aatttaatgg
    15721 aagctactga tcacttgtca gtgatcggag ataagcttat tgatgtaggt agtaagtcta
    15781 ttgaagcagc tggaaaagca caagctatgc aggcccaatt taaacaagtc tttggctctt
    15841 tagaagggga agcacaggac gccgttgagg gaatggctga agaatttgga atgttaccaa
    15901 atacgatcaa gcctgttttt atacaatata cgtcaatgtt taaaggactt ggatatgata
    15961 ccaaagaagc tatggagtta gctggtgata gtactcagtt agcagcagat gcagcagctt
    16021 tttacgataa gtctatggat gatgctagtg aatctcttaa ttcatttata aaagggaact
    16081 acgaaggtgg ggagcaaata ggtttatttg ctaatgatac tcaaatggca gcttatgctg
    16141 ttaagcataa tttgatacca gcgactgaag gagcaaaaaa agccagtgaa gagtcattgt
    16201 tagctgttga aaaagcacaa tctaagtatg ctgatgctat taagaaacat ggtgaaggat
    16261 ctttagaagc aagagaggct gctttaaaac ttaaagatgc gcaagataaa ataaatgaag
    16321 aattaggccc acaaacgcaa aaatggtctg atttagatga ggctaccaag caagcagttc
    16381 gagttcaata tgctgaagat atgcaaaaat tagccggtgc tacaggtcag gctagtaggg
    16441 agtctgatgg tttagaaaac caaatgacaa gagcaaagca agcgttagaa gatttttatg
    16501 cctcattggg tgaagatata ctgcctgtgt ttatcaaagg gttgcaagca ggagcgaaag
    16561 ctttgcaagg attagccgaa tggtggagta aacttgatgg gccaatgaaa aatttcattt
    16621 tagctcttgg aggaattcta gcattattaa gcacattagc tcctgttata accgcagttg
    16681 ttacgatagt tggcacattt ggttctacag ttttgctgcc aataatagga attatagcgg
    16741 gcgttgcagc tgtaatagcg attgtaatta cagcgtttca aaactggggc gcaatcactg
    16801 actggtttag tgatttatgg aaaaaattta ccgattggtt aggtgatacc tgggaaagta
    16861 taaaagaaag tgcctcatca gtttgggatg gagtcaaaga aacctggtct ggatttgtag
    16921 attgggttca agaaatttgg caaggagttt ctgattggtt tggagagtta tggagcggat
    16981 tagttgaagg agcttccaac atctggcaag gagtccaaga gacttggcaa gcattcgttg
    17041 attgggtttc aaatatttgg aacggagtca aagaagtatg gtcgattatt tgggcagaca
    17101 ttgtaggaat tgttcaaata ccatggacat taataacgtc attgattcaa gctggtatta
    17161 atattatcgt gggtatcttt gatgtagctg gacagttatt aggcgcagct tggcaagctg
    17221 tttggacacc tatttctgat ttccttaaaa atacttggga tactatgaca caatggataa
    17281 gcatcgcttg gaatggaatt gtaactacat tccatactat atttgatcca gtagtggcat
    17341 ggtggaatgg tatatggaca tctattagta ctacggcttc aaatatttgg aattcaatta
    17401 gtgcaacagc ttctagtatt tggaacagta tcaagaatac aatcactagc ttggtacaag
    17461 cagctgctac agtaattcaa aatatttggt caactgtatc tagttggtta ggtggaattt
    17521 ggaattcaat cagctctaca gcatcaaata tctggaatag tgtgactagt agtataagca
    17581 atgctataaa cgcagctaaa agtgccattc aaagtgtttg gaatagtata tcttcgtgga
    17641 tcagcggaat ttggaacggt atcaaaaata ctgctttgaa tctttggaat ggaattacaa
    17701 gcactattag ctctaaagta aacgatggaa aaaatgcaat ttcaagcggt tggtccaatc
    17761 taacaggtat tgtttccgac atattcaata atgttaaaag tacaattgct aacatatggg
    17821 aaggcatcaa aaagactgtt agcgctccaa ttgattggat tagagacaaa atcagtggca
    17881 tttttgataa tttgaatatt tctataccac atattccgtt accggaattt atcatggaag
    17941 gcagctttaa cccgctaaaa ggtcaaatcc cccatttgcg tgtaaaatgg catgctaaag
    18001 gaggtatctt tactaaacca actttactag gtggaatgaa cggtgtcggt gaagcaggac
    18061 ctgaagcagt tttacctttg aaaagatctg ttttgcaaga aattggtgat cgtatcttga
    18121 gtagcacatc agtttcatct agggcacaaa cgattcaacc tgtgaataac tacgaattca
    18181 atttcacaat tgatggtaac gcagatgagg ttactatgaa gcaaacaact caacaaatca
    18241 ttgatagcat tacaaaaatt caaaatgata atgcttcggc atggcgttaa acaggagagt
    18301 atttctcctg tttttttagt attaaaaagg atgtgaaaaa atgactgatt gtatacattc
    18361 tataatcgat ggatttcctg attatttgca taaattggct ttagctgaaa gaccaaccat
    18421 accttctcca aaaagacaga gagttgaaac ttctgtttta ggtaggttag gtggcttagt
    18481 acaagattac tcgtttgaag atatgtcgtt tacattgcac tataactatt tagaggatgt
    18541 ggaagaccat caagcgttca agcaatcgtt ttatatcatg cgtcattggt taaactatgc
    18601 aaagaaatta gaattctctg acgatcccaa cgtctattac gttatccaga ctatcgatat
    18661 tggggatgca gaaaacgata ttgttgaatg gggagagttc gatgtaaata ttactgcgaa
    18721 accattcgca agagttcaag aagatgtacc tataaccgta gataaaccac agtcatttaa
    18781 cttgctgaat aatagtttag aagaaagttt tccaaagatt atcatcactc cttcagctac
    18841 ttcatgccag ttcatcttaa atgattatgt gtttagtttc gaaggcttag tagcaggaac
    18901 tgatgtagtc attgatagtg atttgatgct ttgctacgaa gagcaatcgg acggagatat
    18961 tttagatcgg tccaacaaaa tgaagaccat gcaatatcca acgttgcaag tggatattaa
    19021 tcattttaat tgtactggtt tgagcaaaat acaaatttat cgtaacgggt taaggtaggt
    19081 gaaatagatg atcgataatt taataactat ttacgataaa aatgacgcga ataatttagc
    19141 tgaacattta tatgatacgc aaggtttagg cgctttgtca gactggttaa cagctactgt
    19201 tagcaataaa ctaaacggag ccgagatatt tcagggtact tattcaataa acggaactaa
    19261 tgcagatttg attgtagaag gacgtattat tcagtgttat gtagatgaaa atcgagcaaa
    19321 acagcgtcta cggatttatt atgcaaaaac ttctgtaata ggcaatacga tagaagtaaa
    19381 agctgaacct attttcaatg atataagaaa atcggtgttg aataaatatg acagcggaac
    19441 agaaaagatc actgctactc aggcatggca aaacgcaaaa gttttagcga aaccagctat
    19501 cccttcgcag ttttctttct cgtcattagt agatacgctt gctaatgtga agatagaaaa
    19561 ggcgaatttt ttagaattct ttggtggaaa agagggatct attctagatc gatttcatgg
    19621 tgagtttcta aaagataata acacattacg tcatgaaaaa agtctaggta cggatcataa
    19681 aatcaaagcg atttatacta aaaacttaac tggtcttgac ttagagattg atgctcaaag
    19741 tgttttagtt ggagtttatc cattcattag cagctcttca gaaggagaag acgagatcac
    19801 tctaccagaa gaagttattt tcacggatta cgtggatgat tatcctgctg gatatgtttc
    19861 ttttgttgat tttaaagaca aagcgactga tgtagccaca ttaagggaag ctgctaaaga
    19921 ctggttgaaa acaaacatag ataaacaaaa accacaagtg agtggttcga ttgaattagt
    19981 accattgagg catcaaagag gctatgaaaa atttgttgat ctagaaaaag tttcgatggg
    20041 tgacggagta gatgtgtatc atccacagtt aaaagtgaat atgtcagcga gaattgtgga
    20101 atatacgttt aatgttctaa ccaattcata cgataaatta gttgtaggaa acgtcaaaac
    20161 aaacttctta gaaaacacag agaataatgt cagcaatttg attaatgatg ccattgatca
    20221 attgaaaaat ggtggcgaaa tcagtgattt aatcaatgat attgtagatc atcaaactga
    20281 tatgattact ggtcaaaatg gtggttacgt tttattagat cctaaagaag cccctagtcg
    20341 tattttgatt atggacacac cagataagaa tactgcacgg aacgttttgc aaatcaacaa
    20401 cgctggtatt ggtttctcta aaactggcat taatggaacg tatgacaccg catggacgtt
    20461 agatggcgga ttcaatgcct cgtttattac agctggtgag atagtaggga ttactattag
    20521 aggtactaca ttaattagtg atggcactga ttatagaaca agtatagcta atggcaaaat
    20581 gacttggtac tcaaaaaaag ttaacaaaga tattatggag ctagaagcac gtgattatgt
    20641 aagtgctgat gccggcattg tatcatacac catgaaaact ggtggtggtt tcatgattag
    20701 aaatccacag ggtaacttgg tttttagtac gtgggataat ggtaataaca gaccgttttt
    20761 atcttttggt gcgccaaatt tcagatacag taatgctagt tatataaatg atggtgacgg
    20821 taattcttta ggtattgatg gtagtgcagg taattcatgg gaatttaaag tagctggtag
    20881 gactatgaaa tttactagtg acggtatgct aacgttgcca ggttgttttt ttggttcatg
    20941 ggaagatggg aaacttgcta gatttgaaca atcaacggta caagtatata aagattttac
    21001 tgttagaggt actaaaaact caaccgtacc aacagaacat tatggacaac gactattgaa
    21061 cgcttatgaa actccagaat attatttcgc tgattatggg gaagccgtta caggtgatga
    21121 tggtaaagtt cgtgttgata ttgaccccat gtttgctgag acagtaaatc taagtcggta
    21181 tatgacacat gtgacaccta cagaactagt tttgtgtgct gttactcatg aagatgttga
    21241 ccatttcatc attgaaacta gtaagccaaa cgtattggtt agatggaatt tagtggcaca
    21301 tcgactaggg tatgaagatg ttagattaaa agaggataca gcatatgata gcacagtgct
    21361 tgaccaaaaa cgtttttaaa acgaagacaa ggaggtatat aaatggctag cagtttatat
    21421 aatttggctt tagatttcag caaagaatta aactacacca aagctattat ggctcgtcaa
    21481 ggtgataaag ggatcacggt gactgttaaa ccgtttttaa atggcttgca gatggatacg
    21541 agcggcggaa catttacttt aaaaggaact acgccatcta accgttacgt agatagtgtc
    21601 gctactagtg taactagtga agaagtcaca ttttctcttg atggcacatt tatgagtgaa
    21661 gcagggtatt ataaacactg ctatgtagaa tatagaaaag acgatcaaat tttaacgacg
    21721 caagatatca tttttttctc actaggagtg tctgacattt cgcaaggcca agccgatgaa
    21781 tatgtttcgc aattagaaga attgattcga aagtataacg aaacttttga tgcttttctg
    21841 gctgaaatta aaggtagaat ggatagctta aataaacaga ttactgattt aactggtcaa
    21901 gctaaaacac tacaagacaa gttagatgct ctgaaagaag aaatttctaa attaggtaac
    21961 ttacatgtga tgtactccaa cagcatcgac ttcgggggct atgattatag tgggaatccg
    22021 aatttaatgt ccaaactaaa atcgagcgat tttaacgttg gttaccacgg gtcactaact
    22081 aatgttaacg gaatgttaca ttttatgtct gatggtacag gggacattga tatgtttacg
    22141 cgtattaata cacctcagct tgctagtggc aaaacatata ctctgagtgc aaaagttcga
    22201 tttgatgaag gaactacagg agctattaat aaattacgtt tggtatatcg tacatcacca
    22261 ggagaaaata tattattgga agcaaataat acaactatta caatagatga tgtagggaaa
    22321 gaaataacaa tcaaaggcac agctaacgtt aattatcaaa tcacaaattt agatcgtttt
    22381 tatattagtg ttagtttttc taacagggat aaaataaatg gtggatttaa attgtacgac
    22441 atcaaaatcg aagaaggctc aacagccact ccgtatcagc caaacttact agatgaccct
    22501 tactgggtag gtaaaacatc gttgggtaag aatattgcaa ataaaggtac gacgttccca
    22561 attaaatcta gcgcgtacga aatatataaa ggtaacacgg aagaagagct tataataggt
    22621 caaacgtata ctatcacgct taaaggaaca aaacccgcaa gtcaaacctt tgtagcatat
    22681 aattattgga acgttaattt tggagatcta aaaccagttg agggattgac agacgtatgg
    22741 tctctaacat tcacaccaac gaaacttgaa ccgggtttgc ctaaagacct tcgcattttt
    22801 cagtcgccta aagaaacggc cggcgcatgc cgaattgact ggctcaagat cgaaaaaggc
    22861 gacacacgaa ccccgaatat tgagcaatat aaataccgag gaatcggcat gcgagactca
    22921 aacaatccaa aagattatgt atgggatcta gaacctagct atgttgagaa taacctagct
    22981 acagatatca aggttgctga aatcacaggt aaagcaaaca attataccga tgggaaagta
    23041 tcggagatta attcgcagtt gactgcttca attaatgaag tagacaccac agctaaggat
    23101 gctcaaacaa aagcgaatgc taatgcgact gctatagatg aattagacaa taagatcgat
    23161 gaacgcatta atgatacagc tactaccaca ttaacagtta caaacgggaa taccggatca
    23221 gcaaagcttt atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa
    23281 agcagtggtg gaaatgattc aacgatacta acaattccag aaggctatcg gccaccaatt
    23341 agttttgagc aactggttgg ctc
//
LOCUS       DASJST010000033        23158 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJST010000033 DASJST010000000
VERSION     DASJST010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.033"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            32..889
                     /locus_tag="U4B05_001041"
     CDS             32..889
                     /locus_tag="U4B05_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN2050193.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1004..1924
                     /gene="pstC"
                     /locus_tag="U4B05_001042"
     CDS             1004..1924
                     /gene="pstC"
                     /locus_tag="U4B05_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN2050194.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1924..2808
                     /gene="pstA"
                     /locus_tag="U4B05_001043"
     CDS             1924..2808
                     /gene="pstA"
                     /locus_tag="U4B05_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN2050195.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2823..3629
                     /gene="pstB"
                     /locus_tag="U4B05_001044"
     CDS             2823..3629
                     /gene="pstB"
                     /locus_tag="U4B05_001044"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2050196.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3645..4403
                     /locus_tag="U4B05_001045"
     CDS             3645..4403
                     /locus_tag="U4B05_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2050197.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4416..5093
                     /gene="phoU"
                     /locus_tag="U4B05_001046"
     CDS             4416..5093
                     /gene="phoU"
                     /locus_tag="U4B05_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN2050198.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5331..6362
                     /locus_tag="U4B05_001047"
     CDS             5331..6362
                     /locus_tag="U4B05_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2050199.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSISIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6359..7126
                     /locus_tag="U4B05_001048"
     CDS             6359..7126
                     /locus_tag="U4B05_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2050200.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7226..8794
                     /gene="liaX"
                     /locus_tag="U4B05_001049"
     CDS             7226..8794
                     /gene="liaX"
                     /locus_tag="U4B05_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN2050201.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9091..9411
                     /locus_tag="U4B05_001050"
     CDS             9091..9411
                     /locus_tag="U4B05_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN2050202.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9411..9761
                     /locus_tag="U4B05_001051"
     CDS             9411..9761
                     /locus_tag="U4B05_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2050203.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9928..10866
                     /locus_tag="U4B05_001052"
     CDS             9928..10866
                     /locus_tag="U4B05_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN2050204.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10881..11711
                     /locus_tag="U4B05_001053"
     CDS             10881..11711
                     /locus_tag="U4B05_001053"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN2050205.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11755..12780
                     /locus_tag="U4B05_001054"
     CDS             11755..12780
                     /locus_tag="U4B05_001054"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2050206.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12795..13733
                     /gene="galU"
                     /locus_tag="U4B05_001055"
     CDS             12795..13733
                     /gene="galU"
                     /locus_tag="U4B05_001055"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2050207.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13858..14298
                     /locus_tag="U4B05_001056"
     CDS             13858..14298
                     /locus_tag="U4B05_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN2050208.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14301..14903
                     /locus_tag="U4B05_001057"
     CDS             14301..14903
                     /locus_tag="U4B05_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2050209.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14971..16077)
                     /locus_tag="U4B05_001058"
     CDS             complement(14971..16077)
                     /locus_tag="U4B05_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2050210.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16287..17288
                     /gene="ccpA"
                     /locus_tag="U4B05_001059"
     CDS             16287..17288
                     /gene="ccpA"
                     /locus_tag="U4B05_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN2050211.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17624..19969)
                     /locus_tag="U4B05_001060"
     CDS             complement(17624..19969)
                     /locus_tag="U4B05_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2050212.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20248..21150
                     /locus_tag="U4B05_001061"
     CDS             20248..21150
                     /locus_tag="U4B05_001061"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN2050213.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21363..23138
                     /locus_tag="U4B05_001062"
     CDS             21363..23138
                     /locus_tag="U4B05_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN2050214.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7372 a         4123 c         4894 g         6769 t
ORIGIN      
        1 aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat tcttaccaat
       61 aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag gagaatccat
      121 tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg aaacttacca
      181 gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc
      241 tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc
      301 ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga tcactcctat
      361 tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa aactatttac
      421 aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag ttatattgaa
      481 ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg acggagaaac
      541 agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg ttgccgacac
      601 acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag atacgttgaa
      661 tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc
      721 atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat ttttagacta
      781 tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata tccctgtttc
      841 tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat ctttacacaa
      901 aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg ttcaatttgt
      961 acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag atgtgaaaaa
     1021 agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat tcatcagttt
     1081 tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg tagctagtaa
     1141 aggattggcc acctttttta ctgataagat caatgtattt gattttctgt ttggtacaga
     1201 ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat
     1261 cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat ttgccattgg
     1321 tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct tacagccggt
     1381 catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt tatcagttat
     1441 cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag ctggtacatt
     1501 tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg cattaaaggc
     1561 agtgccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat ggcaaacaat
     1621 ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg tttttggtat
     1681 ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg cagcattgat
     1741 gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa tgggaatcgg
     1801 aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct
     1861 attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag gagagctgaa
     1921 ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat agtttcgggg
     1981 atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg aataccgcat
     2041 atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg
     2101 atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag ttttcctatt
     2161 tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct aacagatgtg
     2221 attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg tttattcggt
     2281 ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg tgcattggct
     2341 ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt acaagcggtc
     2401 cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga aacagtgata
     2461 aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct aagttctgga
     2521 agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc acctgcgtta
     2581 gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt ccgtcaagct
     2641 gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc tgatggtgtt
     2701 tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt caacttcggc
     2761 gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg agaagaagca
     2821 aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa gagaaagaag
     2881 tagcactata tacggatgat ctacatgtct ggtatggcga caatgaagca atcaaaggag
     2941 tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc ggctgcggaa
     3001 aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca
     3061 ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga
     3121 tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag tcgatttatg
     3181 acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa
     3241 tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac agcttggaca
     3301 aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg
     3361 cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac cctatttcga
     3421 caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc atcatcgtga
     3481 cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc tatctaggaa
     3541 aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt caggcaaccg
     3601 aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca aaagaaatta
     3661 tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa gggatcaatc
     3721 taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc gggaaatcca
     3781 cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg atcaccggaa
     3841 gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt gaactaagaa
     3901 aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc tatgaaaatg
     3961 tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat caagtagttg
     4021 aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg cataaaagtg
     4081 ctttatcttt atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta
     4141 atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt tctactggga
     4201 aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc gtgacccata
     4261 atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat ggtcacttga
     4321 tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag acagaagatt
     4381 atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa tttgaagaag
     4441 aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc agcgccgtgc
     4501 acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa gttatcgaaa
     4561 acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt gaaatgatcg
     4621 cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg aaagctagct
     4681 cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa
     4741 aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc gattatgtga
     4801 aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa gacgcacgta
     4861 cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat aatgcaacaa
     4921 tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac ttgaatgttg
     4981 ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg atcgtctact
     5041 tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc taaactgaag
     5101 ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct
     5161 gttctgcatc aagtaataag aacagtcaaa caaaaaatag ctcctcatat ttttcgtttg
     5221 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggga aagggtttgt
     5281 gacaaaacat gtcacaaacc cttttcatca tttcatagaa aggataaaga atgcaaaaaa
     5341 aaattagcat catcactata ttttttattt gtgtgcttac ttcatgggtt attcagcatc
     5401 aagctgcttt atatgaagta cctgtcatcg agatcgaaaa agtacgaata aaagaggatc
     5461 atcaattgat tacaggaaga ctgatgaaca cggatcaaaa aggagaatcg atttatttat
     5521 cggttcctta tcaaaaaaat caaatagaaa cagcaaatat aaaagcgaga caaatgtatc
     5581 tagtccaaat gcatggagat gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt
     5641 tcttcttcac aagtatctcc attggcgcca tgttgcttat tggcggaaaa cagggtctat
     5701 ttgctttagg aagtctcatc atcaatatcg ttttactttt acttttgttg ctcatcttta
     5761 ctaaaacaga agggatttct ctattaaata tatctatcct gttcgtcgtt atagggatga
     5821 ttattagcat attggcttta gatggatgga atagaagcag tataatcaaa atttcagctg
     5881 cagttacgag tgttttttta gcatttttta tttgtctttt gacaatgaat cattggaaag
     5941 accaaggact gcgctatgag gaactgaatt atctgacacg accgtatcgt tctgtttttc
     6001 tttctagcgt actgattgga acagctggag gaacactcga taccgttatc acagtcattg
     6061 cgacattaga agaattgact aagcgacaac cagaaataac agcgaaacag ttatggcttt
     6121 caggaaggca agtgggtcga gatgtgattg gctcgatgac caatatcctt ttgttcgtct
     6181 atatcagtgg gtcagttcca tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag
     6241 aaacgataga acagcattta tcattggaat ttttacgcgt cattgctggc agcttaggaa
     6301 tagtattatc gatccctatc tcggtcctct tttttctgac tgtaaggagg ctgaaacgat
     6361 gaatgtactt attatacttg ttgggatttt agcaatcagc gtgctactta ttggcggtac
     6421 gcaaggtatg tatattcttt taggcttatt catcaattta gggatctttt tcctgttgtt
     6481 attcggttat catcagaagt ggccgatact ggcattgtcg gtcataggat ttttattgat
     6541 tgctgtggtt atcttatttt ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt
     6601 gagtatactg atctttttat tttgtttcct actattgctt cctatcactg attttttagc
     6661 gatccaaggt tttacatcgg tagaactaga agaattatct ggcttagata agactttagc
     6721 gattgatttt agattactta cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt
     6781 agatgctagt gtagcaattt ctagtgggac attcgaagta tatcaggcaa atccgcaatt
     6841 atctttttac caattgcgcc attctagttt cgctattgct aaaaaaatat tggcgtcaac
     6901 agtaatgaca cttctttttg cattcatggg aagttcttta gcgttgatcc tctggtttat
     6961 tgatttagac attccttttc agcaagtgat caatgaaaaa tcatttgttc tagaatatac
     7021 gatggctatt cttaccacat tgtctgcact acttgttctt cctttaacct gcgtgactac
     7081 agcatatctc tttacaaaaa agaaagaaca actcaagatg gagtaaaagt tacaacttaa
     7141 gtcctatgac atttttactg aatcagtgca taatatggat gtaaggaaaa gcttgcaaac
     7201 aattttgctt aaggaggcaa tttccatgaa agaaagagaa cgtattttag atttagtgaa
     7261 aaaaggaatc ctttcgactg aagaaggctt agatttatta gaaagcatgg caactgaaaa
     7321 agacgaaaag caaataaaaa aagaagcaga caaggtaact gctagtcata aagaaaaaga
     7381 tcaggcaagt caattgatcg ataaacttga aaacggcgaa gaagaaattt ctgagccagt
     7441 tgatccaaag gaaagagaaa ggcaagatca agaaaactta gaaaagatct tagatgaact
     7501 agcaactgaa gcgaataaaa cttcggcgcg tctagatgaa gtaaatgctc aattcgctga
     7561 caataaggca gcacgcaatg agaaacagga agcattgatg cagctgaata caaaagaaga
     7621 attaggcgag ttgacagaag aagaattagc acagcgtcag acgttagaag cagaaatcaa
     7681 agaattagaa gctagtggcg atacactgtt agaagaacaa ttgaaattag aagcagaatt
     7741 aaaagatatc cgtaagaatc aatggtctga gaaaaaagaa acgtttactg ataagtttga
     7801 attgccagat gattggaaag atcaagcaac agatacactg aatcaagtag gcgaaaaaat
     7861 gtcagaagct ggatcacagt taggcaaatt cttgaagaaa acattccaaa ctgtttcaga
     7921 aacagtgaat gacaacatgg aatggaaaga tgtcagctta cgtgtccctg ggattgcgac
     7981 aacgaaattt gagcatgaat tttactatga agctcctgca gcaagtatcc ttgatatcaa
     8041 ggcagcaaac ggaaacgtga cgttaaaaac gtgggacagc aacgatgtga aagttgaagc
     8101 taagatcaaa ctttacggaa aaatgggtgc tgaaccattt gaagcattct ctgaacggag
     8161 ccagattgag gttaatgaag atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc
     8221 agaccttgtt ttctacttac ctaaacgagt atacgaccac gcagctatta aacttctaaa
     8281 tggcaacatc atgattgaaa cattggaagc taaagatatt tatacaaaat ctacgaatgg
     8341 taacatcatc gtggaccaat taactgcaac gatgctggaa gtagaaggtg taaacggcaa
     8401 tatcgatatt cgaaacggga acatcttaga ttctattatc gaaacagtaa atggtacagt
     8461 gacatttggt gcgacagcag aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct
     8521 tacgattaaa gaagataatt tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa
     8581 agtcgcttta ccagatggca tcggtttgga aggacatgct aaaacaagtc ttggaagtat
     8641 caacagccgt ctgtccaatt acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat
     8701 gttgcaattc cgtcgtgttt cagatggtga aatcacgcaa gtgcaacttt caacgacaac
     8761 aggaagtatc tatttaaaag atacagataa ataaagaggt tgtgaaagag atgttcagcc
     8821 ttgagcaata agaagcgatt ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta
     8881 ttgccgatag gctatctctt gaacaccgtt taccaagaga ttatgaacct gccaattatc
     8941 gccgagtcac aaggaataat ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt
     9001 gtgaccgaga attttgcaga aagacaccga ttaagaagaa ggttgtcaca acgattttct
     9061 tcatttaaaa tagtagaaca gaggtgagac atgatgaaaa aaaagttgac aaaatccaat
     9121 aaaaatgttg ttttgacagg gactttagca ggaataggag aatacatcgg gattgatccg
     9181 actgttatcc gtgtgcttta tgtattcctg agcttagttg cttttggaag tccaatcatt
     9241 ttatacattt tattagcttt gattatccca tctgcgcgtt ctgcacgaac aaactatgga
     9301 cataacaatg catattatga aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa
     9361 aaacgcaaag aagcggaaaa aatcgatgat gacgaatgga gtgactttta atgtcgtact
     9421 ttcaacgtat tttagttaat acgttaacat tcatatcatt ggctgttctt tttcctgcga
     9481 tggtccatgt gagcagtatt tggacggccg ttcttgccag cttcgtattg tctgtgttga
     9541 atatgtttgt caaaccgatt ttgacgatac tgtccctgcc ctttacatta gtcacattgg
     9601 gattgtttac gtttgttatc aatgcagcga ttcttaaaat gacctccttt tttgtgggag
     9661 cagaacattt tagtttttct tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc
     9721 ttgtcaatgt aattgtcagt gaacataaca gtagaaaata aaagaaagct gtgccgaact
     9781 atcggcgcag tttttttatt tagaatcagg aatgcgtttt ctattacatt ggtaattaaa
     9841 taaaaacgtt ttttttaatt ggtagtactt ttggctagac aatgataaaa tgatagagaa
     9901 cactctaata gaaatgaggt agcatgaatg tcagaagttg taaaagttag ccaattagtg
     9961 aaaaaattat ctttggaaat cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta
    10021 actggggaca tctcacgccc aggactagag ttgactggtt attttaatta ttattcccat
    10081 aatcgtttac agctatttgg cagtaaagaa atcagttttg cggaacggat gatcccagaa
    10141 gagcgtttga tcatcatgag aagattatgt agtgaagata tgcccgcatt tattatttca
    10201 agaggattac cggctccaga agaaatgatc caggcagcaa atgaaaatgg gatagctgta
    10261 ctgcgttctc ccatctcaac ttctcgttta ttaggagaat tatccagtta tttagatggt
    10321 cgccttgccc ctcgaacaag tgtccacggt gttttagtgg atgtttacgg attaggtgta
    10381 ctgattcaag gtgacagtgg gataggaaaa agtgaaacag cgttggagtt gatcaaacgc
    10441 ggtcatcgat tgatcgcaga cgatcgagta gatgtttatc agcaagatga attaacggtg
    10501 atcggagaac cgcctaaaat cctggaacat ctaattgaaa tccgaggaat cgggatcatt
    10561 gatgtaatga atctttttgg tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct
    10621 gtttatctgg aagcatgggc aaaagataag aaatacgacc gtctgggctc tgaagacacg
    10681 accgttgaaa tcgcggaagt gggcattcca caaataaaaa tccctgtcaa aacaggacga
    10741 aacgttgcaa tcatcattga agtagccgcg atgaatttcc gtgcacgtac aatgggcttt
    10801 gacgcaacga aaacatttga agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac
    10861 gactagcaag gagtgacaat atgttagcac aaatcaatcg agttgctttt gagcttgtag
    10921 ggattcctat ctactggtat gcattgatca tcgtatcagg tatcattatc gccatgtggc
    10981 ttagcagcag agaggctgtt cgggtaggat taaaagcaga tgatgtgaca gattttatgt
    11041 tgtggggatt gcctatctcc attattggtg ctcgcttata ttatgtttta tttgatcttc
    11101 cccagtatat agctgatcca atccaaatat tcaatatccg ttcaggaggc ttagctattt
    11161 atggcggact gattgcaggc gcgatcacgc tgtatttttt tactaaatat cattttattt
    11221 ctttgtggac ttttttagat atcgctgcac caagtgtatt attagcacag gcaatcggtc
    11281 gttggggaaa tttcatgaac cacgaagcat atggaccaga cacgacgcga agcttcttag
    11341 aaagtctgca tttaccgcaa ttcatcattg ataatatgta tatcgaaggg atctatcgtc
    11401 aaccaacatt tctttatgaa tcactttgga gtttgattgg atttattttg cttttgtttt
    11461 tacgaaagaa accacacctt ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt
    11521 atagtttcgg gcgtttcttt attgaaggat tacgtacaga cagtctttac tttttaggtg
    11581 tgatccgtat ttctcaatta ttatctgctg tgttatttgt cggtacattg attctattta
    11641 tttggcgcag aaaaaaagga aatttaccgg attatgaccg ctcattagga aaaaatcaac
    11701 aaattatctg atttttagag cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat
    11761 tatagagtag cggttttagg accaggctct tggggtactg ctttggcaca agtcctcgcc
    11821 gaaaacggac atgacgttcg gatttgggga catcgtgctt cacaagtaga agaaatcaac
    11881 acccaacaca cgaatcaacg ttatttacca gatcattatc tacccgattc catccaagca
    11941 ttcaccaata tggaagatgc agtaaaagac gcagatgcgg tgctattcgt cattccaacg
    12001 aaagcaatac gttctgttgc aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc
    12061 atccatgcaa gtaaaggatt agaacaggga acacataaaa ggatctctga catcttgtta
    12121 gaggaaatcc cagcagaaaa aagaaaagct gttgttgttc tttctggacc aagccatgca
    12181 gaagaagtag cgaaacatga catcactacg atcacagctg caagtacaga tgcaaaagca
    12241 gcaaaatatg tacaagagct gttcatgaat aattattttc ggatctatac caatcctgat
    12301 gtgatcggtg tagagatggg ggcagctttg aaaaatatta ttgctattgg tgctggcgct
    12361 attcacggct tagggtttgg cgataatgca aaggcagcaa tcatgacgcg cggattagct
    12421 gagatcagcc gtttaggtgt agcaatgggt gccaacccgc tgacctttat cggattaagc
    12481 ggagtaggcg atctagtcgt tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc
    12541 aacttgttag gacaagggca gcccctagaa gaggtgctag ccaatatggg aatggtcgtt
    12601 gaaggtgtga atactactca agcagcgatg gaactagccc aaataatgga aatcgaaatg
    12661 cccatcactc aaacaatcta tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca
    12721 aaagaaatca tgttacgtga tgggaaaaaa gaaaatgaat tctcattaac aattttttga
    12781 aggagcgaat gtatatgaaa gtcaaaaaag cagttattcc agcggcagga ttaggaacaa
    12841 gatttttacc agcaacaaaa gctatggcaa aggaaatgtt gccaattgtt gataaaccaa
    12901 caatccagtt catcgttgaa gaagcgttga aatcaggaat cgaagacatc ttgatcgtta
    12961 caggaaaagc taaacgacca atcgaagatc actttgattc aaatattgaa ttagaaacaa
    13021 acctaagtga aaaaggaaag accgaattat tgaaattagt agaagaaacg acagatgtca
    13081 acttgcattt catccgtcaa tctcatccaa agggtttagg acatgccgtt cttcaagcaa
    13141 gagcctttat tggaaatgaa ccgtttgtcg ttatgctagg cgatgatatc atggaagatg
    13201 aagtaccatt gacacagcaa ttaatcgatg attacgaacg tactcatgct tctacgatcg
    13261 ccgttatgca agttcctcat gatgaaactt caaaatacgg aattatcaat ccaggagaag
    13321 tattggaaga tgggttatac aatgtcaaaa actttgttga aaaaccagat ccaagcaaag
    13381 caccaagtga tttagctatt attggacgtt acttattgac tccagaaatt tttgatgtat
    13441 tgtccgagca acaaccagga gccggaaatg aaatccaact aactgacgca atcgatacat
    13501 tgaataaaac acaacgcgta tttgcccgta aattcacagg taaacgttac gatgtcggtg
    13561 acaaatttgg ttttatgaaa acaagtattg aatatggtct aacacatcca gaagtaggtg
    13621 cacctttacg tgagtatatc ctgtctttag gtaaaaaatt agaagcagaa ggaagcaaga
    13681 tcgatcataa tccaaaagga ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt
    13741 ttctaacgag cttcagctgc taattgaata aaaagtagtt gaagctcgtt tttatttgtt
    13801 taaaaaacac gaaaatgcta tccttataga aaagagagac aagaagggag tttgatcatg
    13861 tcaggaggag aaattgcagg attgatcgca gcaatcgcct ttgcgatact tgtcgttttt
    13921 atcgttcgtg tgttgttgca agtttctaaa actttagaaa aagtagatca gactgtagca
    13981 gaagcaaata cgacgatcga aatcgtcaca aaagacgtag atcttttgtc tagacaagta
    14041 gaaggcttgc tggtcaaaag taatgaactg ctcttggata tcaatggaaa agtcgcaacg
    14101 atcgatccat tgttcacagc agttgctgat ttaagtgaaa gtgtatcaga acttaatcat
    14161 tctagtaaaa acatcgcgac gaaagtcggc ggaatcggta aaacgacagc taaagcgacg
    14221 gttgccggaa aagtcggtgg gacagcgatg aaatttttta aaaacaaaaa acatactgaa
    14281 acgacaggag gaaaataaaa atggctaaaa aaggtggatt tttattaggt gcagtagtcg
    14341 gaggaacagc cgcagcaatc gccgcgttat tgttagcacc aaaatctggt aaagaacttc
    14401 gggatgaatt agctgcccaa gcggatgatt tgatggatat ggcttcagat tacacagata
    14461 tggcgaaaga aaaatcgact gagttaacag gtattgcaaa agaaaaagcg caagatcttt
    14521 ctcaacaagc aggtgatcta gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg
    14581 aagcaggaag tgaatctgac gatatcttgt taaatcttaa agaacaatca gctgacttat
    14641 ctaatcgttt caaaaaaacg gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta
    14701 ttgctaaaga tacttcagaa gatatcatga ttgatgtaaa agacgctgct ggtaaagcag
    14761 gagatactgt caatgaagga atgtcagaag caaaaccagt tgcagatgac acaaaagaaa
    14821 tgatgaaaat ggcaaaagaa gatgcgaaag aagctaaaaa agaaataaaa gatacaatga
    14881 cagattcatc tacaaatcac taaaatcact aaaatcacaa atttatagaa taaaaaggtg
    14941 ggagcggact agctctcacc ttttttactc ttatttttct aaaactagta attcttttgg
    15001 tgtagtcgtg aatggcacac agccatcgct tgtcacatat acacagtctt cgatacgtac
    15061 ccctactttt tcaggaatat agattcctgg ttcaagagag aagcacatcc cttcttcgat
    15121 tactaagtca tttccttcta caagggaagg gaattcgtgg acggttgttc cgatcccgtg
    15181 acctaaacgg tgattgaaat attcgccgta gccatagctg ttgatcacgt ttcgtgcgat
    15241 ttgatcaagt tcacctgctg tcacacctgg acggacggct tcttgtgcgg caagctgtgc
    15301 ttcaagagtg atgttgtata ttttttcttg gaagtcggtt ggtttttgat aagcaattgt
    15361 acgagtagca tcactgcaat agccattcca aacaacgcca agatcgaata acacaagttc
    15421 attcggtgaa atcttcgtgt ttcctggtgt accatgaggg cttgcagcat ttgctcctgc
    15481 aaggactaat gtgtcgaaag acatttgtga aacaccttgt tttttcaatt ggtattcgat
    15541 ttctgcaatg atcgcttgtt cagtcacgcc ttctttcact gctttaaagc caatctcaaa
    15601 tgcaacatcg gcccatttac cagcttcgat cagtttgtct ttttcctcta gagttttgat
    15661 cagttgtaga cgttggatga tggaagctag atcagtgtaa gaatccgttc ccgtcaagat
    15721 atcagagata gcatcgaagc gctcgacagt caaggcgttc ttttcaatcc ctacattgcc
    15781 aggctgacca tagcgagcac gaagttcttt tccgatgatc tcccaagggt tttcgctgtc
    15841 taaatagcca cggacatcat acgcccaact actgttttga gcatcttcaa cttccaaagc
    15901 aggagtgaaa aggaaaggat cttgttcaag agggataaaa agagctagta cacgctcatg
    15961 aggatcactt tcgtatccgg aaaagtaagc gatgtggcca gggtcgctga tataagccaa
    16021 ttcgacttgg tgttgtgcca tccattgacg tagttcattg atttttacgt tattcatttg
    16081 gcaacctact ttctaaatta attttgtatt caataattgt accactgatt tattaaaaga
    16141 ccaacgttct aaaaactgaa aaaagtaaaa attttcatga aaacagagat aaaacggttg
    16201 caattcttga gattatttga tattctattt tagaaatgta agacgtattt tcagaaaaca
    16261 tataaaatat gaataggaga acacaaatgg aaaagcaaac aatcactatc tatgatgtag
    16321 cgagagaggc aaatgtttcg atggctactg tttcacgtgt cgtaaatggc aatccaaacg
    16381 tcaaaccagc aacaagaaaa aaagtcttgg aagttatcga tcgcttggat tatcgtccaa
    16441 acgcagttgc gcgtggcttg gcaagtaaga aaacaacaac agttggggta atcatccctg
    16501 atgtcagcaa tatgttcttt gcttctttag cacgtggtat cgatgatgtg gcaacaatgt
    16561 acaaatacaa tattatctta gcaaattcag atggtaacga ccaaaaagaa gtcaatgtat
    16621 tgaataactt actggctaag caagtggacg gcgtgatctt catgggtcac catatcacag
    16681 acgaaatccg tggagaattt tcacgttcaa aaactcctgt tgtattagct ggctctattg
    16741 atccagatga acaagtaggt agcgtaaaca ttgattatgt atcagcaaca aaagatgcag
    16801 taaacaaatt ggcaaaaagc ggcaataaaa aaatcgcttt tgtcagtggt gcgttgatcg
    16861 atcctatcaa tggacaaaat cgtctgaaag gctataaaga agcattaagc gaaaataacc
    16921 taccatatag cgaaggattg atctttgaag cacaatacaa cttcaaagat ggtctatctc
    16981 tagtcgatcg gatccataat agcggtgcaa cagctgtcta tgtttcagat gatgaattag
    17041 ccatcggtat tttggatggt ttattagacc gtggcgtgaa agtaccggaa gaattcgaaa
    17101 tcatcacaag taacaattca ctactgacag aagtagctcg tcctcgttta acaagtatca
    17161 cacaaccatt atacgattta ggggcggtat ctatgcgcct attgacgaaa atgatgaata
    17221 aagaagaagt cgaagaaaaa acgattatct tgccttatgg aatcgaagaa aaaggatcta
    17281 caaaataata aaaggacaac cattcagagg ttgtgagcct gccgtttaac tctgcgttac
    17341 aaggagcaat ttcaaaaaac agcatttcat gtttgatgaa tattgtgact cgtgattcgg
    17401 agttggcagg agaacaccgt ttattcggga aaaggggtta tgacaaaatt ttgtttaatc
    17461 cttttttttt gtcttaaaaa agagagaaag tggttgaaat tacatttatc gtatataaaa
    17521 caatccgtgt atagatgctg cattatgttc accaccatca aaaaaataag ggactgtgac
    17581 aatactattg tcacagtccc ttattactgt gtattctatt aaatcaatca tctttctcat
    17641 ctgattcttt ggttgcttga ttcgctcttg cataagctga agcggtgttc cagtaatctg
    17701 tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc ggtccatttt
    17761 tcgcccatag cgattcaact gtttttcctg gtgtttggat ggatttttta ttgacagtaa
    17821 cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca tcagatgata
    17881 attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct tgattgatca
    17941 gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca gtattgtcat
    18001 cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc catgaatctc
    18061 ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga tttccgttta
    18121 agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca ctgcgcatca
    18181 tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg ctttcgtgtt
    18241 tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct tcttcataga
    18301 cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg acatctactg
    18361 tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc atctttgcca
    18421 agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct tgatataagt
    18481 gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg aaggtgttcg
    18541 atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct tgccaagttg
    18601 ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt gcaggtccat
    18661 aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag gcatggttat
    18721 tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca gttctgttat
    18781 ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac atgtatccat
    18841 aattttctac gccattttgc aatgcagcat agacatcttt atcgattgtc gaatggattg
    18901 tatatccttt attttggatc gtttgctgtg ccttttgata ataagcgtca ctgaccgatg
    18961 aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt cttgttgctt
    19021 cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt tgttgtggta
    19081 gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc gtaatttggc
    19141 cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca gaaagatctt
    19201 cttttagcgc acctgtgttc gtgtatggac tatacgtgat cgggctttgg ggtaatccag
    19261 cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag attcctcttg
    19321 cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat ggtgagacat
    19381 tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt gcagaaagaa
    19441 tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc tgtttgacta
    19501 attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca gatactaatg
    19561 ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca tcctctgtgc
    19621 tgataattgc agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc gtgcgcatca
    19681 gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt gacgtttctg
    19741 taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg tcttcgacga
    19801 gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc aaaagaagaa
    19861 cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga acaaaccatt
    19921 tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa agggtcacct
    19981 caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa cagacaaggc
    20041 tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg atctttttta
    20101 taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag aacagggcaa
    20161 agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat tgttagtaac
    20221 aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag catgagcgga
    20281 aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc gacttttatg
    20341 atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat gaatattgat
    20401 gccggaaaag cgtacatggc agaaaacggt ctaacaaata tcgtcgttca tgccccttat
    20461 atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt ccaatttctg
    20521 agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact tcacccaggt
    20581 gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg cttgaatgaa
    20641 gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg taaaggtacg
    20701 gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa attgaatgaa
    20761 aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa tattaaagaa
    20821 gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg gcttaaagta
    20881 ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca cgccaatatt
    20941 ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc aatgtttgac
    21001 aatatttcga aaatcttgga aactccctat gtcggcaaag ataaaaagaa agcttacgca
    21061 ccatatggaa aagaaatcgc catgttcaaa gcacaaatat tcgatccaga agtgtttcct
    21121 gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag caaaaaagga
    21181 ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtgcttt ttgcagtctt
    21241 tttcatcatg catcttctcc cccctctttt taaatttaga caaacagggt atactggtag
    21301 aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg aaggacgaaa
    21361 agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat acatacttta
    21421 ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt aaagcaatct
    21481 atggaacaga catgtcggca acctatgatg accagctgct gattcgctat gctcaaaata
    21541 ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa ggaatctcct
    21601 acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt ttgttcggca
    21661 tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatc gtgaagaaca
    21721 agtatcttct tttaagtttc tatttttatt ttttgttttc tccgattagt tttacacatg
    21781 aatattccac tcgaacgtat cgtaatgccc tcgtgattcc ggctgttgag atcattatcg
    21841 cttgtttact agctatttat taccgtaaat atagttccag caaaaaaatg ctgccgtggt
    21901 ttttagggct agctgtttct tttcctttct tttggttcat tcgcgaagat tccatctggc
    21961 ttcttccttt tactgtagtc gcgttaggca tcagtttcgt ccagatcttg ctaggacaag
    22021 acttttcttt caaagaagca aagaagttaa aagaacgacc atttctcctt tcgaaacaag
    22081 aatggctgaa actttcattg tttttactcc ctctagtcgg tttctccatc gtctatcagt
    22141 ggatcgaata taaaaacgag acaacatata gtattgccgc catcaatgac cgttcgagtg
    22201 gcgaatttgg acgtttgatg aaacagctga tccgaataga tgacgaaacg aacttaaata
    22261 aagaaaacag tgagatatgg gtctcgcatg ctgcattaaa taaagcatta gacgcatctc
    22321 ctacatttca tgctgtttct actcgtattg acgaattgta tacgactcat gcgtggaccc
    22381 aaggtggaaa agtaaaagaa ttaccaggag atatcatttt ttgggcattg cgagatgtgg
    22441 taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt tggaaaaaag
    22501 tcaatcaaga attagaagaa gcctatcaaa agggaactat caaaaagaaa aaagaattct
    22561 atctgatggg atcaggggat ggaaagacga tgaatgatct gccaatcgta tttgcttttc
    22621 tgaaatcagg atatgcctat gcatggcgat acaaagattt ccatcaaggg gggaattata
    22681 gtttcggtac gcaagaacaa gtcgaacagg cccaagaatt attgaatctt ccactaatgg
    22741 ataattggat cgatagcagt gatccgagaa cacctgattt ccagttgaca aaaacagcaa
    22801 aagtcgcgaa tgtcatgata aaaatttacc aagttctggc cattcccgtg ctgattgctt
    22861 caatagcagg gatattactt atgatactag gaaccgcatt tgcaaaagct gatcgtgcct
    22921 attatagttc ctctttgatt gtagtcaccg gattaatact gacacaattg gtttttcttt
    22981 tcggcgtctc gtggttttgt tcttatgcgc cagaaaaaaa ggattatttt ttaagtgtct
    23041 acactggcgc aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg ctagttttca
    23101 gtaaattgaa tctgaaaaaa gctttatctt ccaaataaaa acatactaag attagagg
//
LOCUS       DASJST010000034        23116 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJST010000034 DASJST010000000
VERSION     DASJST010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.034"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            242..2713
                     /locus_tag="U4B05_001063"
     CDS             242..2713
                     /locus_tag="U4B05_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2050215.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2816..4552)
                     /locus_tag="U4B05_001064"
     CDS             complement(2816..4552)
                     /locus_tag="U4B05_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN2050216.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4549..5253)
                     /locus_tag="U4B05_001065"
     CDS             complement(4549..5253)
                     /locus_tag="U4B05_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2050217.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5384..5887
                     /locus_tag="U4B05_001066"
     CDS             5384..5887
                     /locus_tag="U4B05_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN2050218.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5847..6185
                     /locus_tag="U4B05_001067"
     CDS             5847..6185
                     /locus_tag="U4B05_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN2050219.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6206..7624
                     /gene="ffh"
                     /locus_tag="U4B05_001068"
     CDS             6206..7624
                     /gene="ffh"
                     /locus_tag="U4B05_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN2050220.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7736..8314
                     /locus_tag="U4B05_001069"
     CDS             7736..8314
                     /locus_tag="U4B05_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN2050221.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8318..8839
                     /locus_tag="U4B05_001070"
     CDS             8318..8839
                     /locus_tag="U4B05_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2050222.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8918..9472)
                     /locus_tag="U4B05_001071"
     CDS             complement(8918..9472)
                     /locus_tag="U4B05_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2050223.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9725..10000
                     /gene="rpsP"
                     /locus_tag="U4B05_001072"
     CDS             9725..10000
                     /gene="rpsP"
                     /locus_tag="U4B05_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN2050224.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10012..10263
                     /locus_tag="U4B05_001073"
     CDS             10012..10263
                     /locus_tag="U4B05_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN2050225.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10388..11179
                     /locus_tag="U4B05_001074"
     CDS             10388..11179
                     /locus_tag="U4B05_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050226.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11349..11870
                     /gene="rimM"
                     /locus_tag="U4B05_001075"
     CDS             11349..11870
                     /gene="rimM"
                     /locus_tag="U4B05_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN2050227.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11860..12621
                     /gene="trmD"
                     /locus_tag="U4B05_001076"
     CDS             11860..12621
                     /gene="trmD"
                     /locus_tag="U4B05_001076"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN2050228.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12757..13104
                     /gene="rplS"
                     /locus_tag="U4B05_001077"
     CDS             12757..13104
                     /gene="rplS"
                     /locus_tag="U4B05_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN2050229.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13477..14139
                     /locus_tag="U4B05_001078"
     CDS             13477..14139
                     /locus_tag="U4B05_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050230.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14218..17445
                     /gene="fss3"
                     /locus_tag="U4B05_001079"
     CDS             14218..17445
                     /gene="fss3"
                     /locus_tag="U4B05_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN2050231.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17559..17873
                     /locus_tag="U4B05_001080"
     CDS             17559..17873
                     /locus_tag="U4B05_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2050232.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17886..18260
                     /locus_tag="U4B05_001081"
     CDS             17886..18260
                     /locus_tag="U4B05_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2050233.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18270..18614
                     /locus_tag="U4B05_001082"
     CDS             18270..18614
                     /locus_tag="U4B05_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN2050234.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18685..20037
                     /locus_tag="U4B05_001083"
     CDS             18685..20037
                     /locus_tag="U4B05_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN2050235.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20097..21239
                     /locus_tag="U4B05_001084"
     CDS             20097..21239
                     /locus_tag="U4B05_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN2050236.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21264..21518
                     /locus_tag="U4B05_001085"
     CDS             21264..21518
                     /locus_tag="U4B05_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050237.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21774..22958
                     /locus_tag="U4B05_001086"
     CDS             21774..22958
                     /locus_tag="U4B05_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2050238.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22955..23092
                     /locus_tag="U4B05_001087"
     CDS             22955..23092
                     /locus_tag="U4B05_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN2050239.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7685 a         4108 c         4972 g         6351 t
ORIGIN      
        1 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
       61 gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc
      121 tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt
      181 tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca
      241 catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga
      301 taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga
      361 acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca
      421 aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt
      481 gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac
      541 aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt
      601 aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga
      661 ccttgtaata gatgacattg ttattttaga gactggatat caaattccag cagatgcaga
      721 ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat
      781 aaccaaagaa ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta
      841 cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa
      901 gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg
      961 gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa
     1021 tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc
     1081 tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa
     1141 gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct
     1201 ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt
     1261 ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta
     1321 tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa
     1381 aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag
     1441 tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca
     1501 attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt
     1561 ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt
     1621 aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt
     1681 caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa
     1741 tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc
     1801 agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga
     1861 acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga
     1921 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     1981 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     2041 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     2101 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     2161 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     2221 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     2281 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     2341 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     2401 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     2461 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     2521 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     2581 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     2641 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa
     2701 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     2761 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     2821 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     2881 taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     2941 aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     3001 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     3061 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca attagatttt
     3121 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     3181 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     3241 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     3301 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     3361 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     3421 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     3481 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     3541 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     3601 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     3661 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     3721 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     3781 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat
     3841 acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat
     3901 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     3961 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     4021 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     4081 gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     4141 tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc
     4201 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     4261 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     4321 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     4381 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     4441 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     4501 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg
     4561 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc
     4621 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     4681 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     4741 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     4801 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     4861 ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg
     4921 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     4981 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     5041 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     5101 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     5161 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     5221 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     5281 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     5341 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     5401 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     5461 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     5521 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     5581 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     5641 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     5701 cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg
     5761 tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga
     5821 atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc
     5881 tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt
     5941 atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag
     6001 tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc
     6061 ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag
     6121 ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag
     6181 aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca
     6241 ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat
     6301 gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt
     6361 tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc
     6421 acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc
     6481 agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc
     6541 aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg
     6601 tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt
     6661 aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat
     6721 cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac
     6781 ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt
     6841 gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa
     6901 cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga
     6961 tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa
     7021 attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc
     7081 tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta
     7141 cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa
     7201 tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa
     7261 aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga
     7321 tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga
     7381 tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga
     7441 agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa
     7501 aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact
     7561 aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa
     7621 ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta
     7681 tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag
     7741 atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt
     7801 tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg
     7861 attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt
     7921 cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa
     7981 aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt
     8041 gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa
     8101 aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga
     8161 tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta
     8221 tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga
     8281 agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg
     8341 tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga
     8401 cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat
     8461 catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc
     8521 agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt
     8581 cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat
     8641 ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga
     8701 atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg
     8761 aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt
     8821 gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc
     8881 ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa
     8941 aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac
     9001 cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg
     9061 gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc
     9121 cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag
     9181 gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag
     9241 gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag
     9301 caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta
     9361 caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt
     9421 tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt
     9481 tcctccttca aactatctat aataatctta caaaagataa gtaactggag caaataaaaa
     9541 cacttactga caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct
     9601 aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt
     9661 acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata
     9721 aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg
     9781 tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac
     9841 ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg
     9901 gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt
     9961 tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat
    10021 ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag
    10081 ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat
    10141 attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac
    10201 ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag
    10261 taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg
    10321 aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga
    10381 acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag
    10441 ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac
    10501 cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc
    10561 aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat
    10621 gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca
    10681 aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt
    10741 gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga
    10801 aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg
    10861 ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga
    10921 aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt
    10981 agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg
    11041 taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga
    11101 accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt
    11161 ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc
    11221 acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa
    11281 aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg
    11341 gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag
    11401 gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg
    11461 tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca
    11521 aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa
    11581 aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg
    11641 aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta
    11701 aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa
    11761 aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg
    11821 gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact
    11881 ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat
    11941 aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag
    12001 acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc
    12061 tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt
    12121 cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa
    12181 cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg
    12241 acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc
    12301 atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa
    12361 acggattctt attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat
    12421 aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg
    12481 caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt
    12541 gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa
    12601 acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg
    12661 ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa
    12721 gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta
    12781 acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt
    12841 cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc
    12901 aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg
    12961 ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc
    13021 tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct
    13081 cgtatcaaag aaatcagaag ataaacacgg aaaacttcct tggaaatctg aaatttgtga
    13141 aaagtccttg tatatcaagg gctttttctt ttttctctat cacagtttta agccattttt
    13201 ggctgttttg gaccattttt caatcatatt cccttaaaaa ttgtcctaga ttgtccgaca
    13261 ttgactatca gtgtcgtcaa aatagggcaa aatttagtag tcagcttgac gacaaatata
    13321 atacgaaaaa taccaaaaca tttaccgaat ttagcaactt ttgaacccaa gtttctcaaa
    13381 gggtcagcta ttagcaaaaa agacaaaaca tgtacttttc tactaaaaag ttgatgtgat
    13441 aggcttctat ctaaataact attaggagtt tttatcgtga agcaaaatga tggacgtatc
    13501 atttggaaaa atcaaagtga gttaaaactg attttaacaa ttaatgaatt catcgaaaaa
    13561 cacggaatta catctagccg tcaataccag aaaaagctat cagaaaatcc aaattctgca
    13621 ccaagtatgt ggttcattaa caaaaaatat ggatcatggg agaatttatt gattagtata
    13681 ggacgagaaa atactgggta tggaaaatgg gctagaatgt cagaacaaga attattggaa
    13741 atagtagaag cctttattaa gtgcgagaag ataacctctc aacgaatgta cgaacaaaaa
    13801 tcagtaggga agaatattcc ttcacttagt accattaaaa agatgttagg agatatacgt
    13861 ccacttttta aagaaaaaaa tgatggatct cgatttactg atttcgaact gctgttagaa
    13921 ctaaaaaatg aaataatccg attaaaacta caggatgatt tatcaatgac aaagtttcga
    13981 aaactagttc aatccccaaa actaccatca gttgatacaa ttatgaaaag aacgaacaaa
    14041 aattgggagg aattgatggc agagattgga tttgattatc gaagaatcaa aatttataag
    14101 cagagaaaca acctatccaa gacaaagaaa actaaatagt atcactctaa atcagacgtt
    14161 caaaattaaa atttggacgt cttttttatt acccaaaaac gaaaggagat ttattttatg
    14221 aaaaagccaa gatttaagaa ttggcgtttg ctcgcaacac tgagtctatt atgccaaaca
    14281 attggtggat cgcttggccc aacgattgcc tttgctgatg aaattactca tccacaaacg
    14341 gtaactgtgg aattggattt ggctcaccag tatgctgtcg aaggtacatt tagcgatggt
    14401 cgtcccatgt cagaagttac cgttccgcac tatgcagttt acaatggtgt gaagcaagat
    14461 attttctgta ttgagccagg ggtgccgatt tacaatgagt tcactcctgg ttatgagaaa
    14521 aatccattgc ctgatatgtc ggagaaagcg aaattagttt ccgttttatg gaaggaagct
    14581 ggtacggatg tggatacaca tattgtggcc cagaagatga tttggcaaga agttaatggc
    14641 tacacgcttc actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag
    14701 tcaaaaatca atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca
    14761 gtgaaaaccg tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg
    14821 gagtttgatg aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa
    14881 ctagtgatta ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca
    14941 gctaatacag gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg
    15001 gcaattgata agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg
    15061 aagattaaaa aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta
    15121 gactttggaa aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca
    15181 ttggatgaga ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct
    15241 tatacgattg acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg
    15301 acttcaaaaa atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca
    15361 ggaactgatc tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa
    15421 gacagtccaa ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa
    15481 acaccaaaag agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa
    15541 gctagtaatg gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat
    15601 caaaccgtgg ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa
    15661 accactttga caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt
    15721 aaaggagctg aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag
    15781 gcttttaaac cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact
    15841 ttagatgaaa agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa
    15901 gaaaccaaag cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa
    15961 aaggtggatg ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa
    16021 gtcattcgct ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa
    16081 actggcttca atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca
    16141 ggtgccgaag atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc
    16201 aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga
    16261 tttcaaaaaa ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac
    16321 actaagagtg aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg
    16381 gtgaccgttc cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg
    16441 atgctctatg atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac
    16501 ggaggcaaag aattgacaga ccttgattct accgagctag ttgataaatt gagctataac
    16561 ttgcatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca
    16621 aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca
    16681 acgttggcga ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa
    16741 caagttttgg ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat
    16801 gaagcaggtg atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc
    16861 aattgtacgg tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg
    16921 caaaccttta ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat
    16981 gtactggatg gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat
    17041 acgaccaaag aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc
    17101 actaaaaccg tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt
    17161 accttcaaag aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat
    17221 ctaaaagaaa aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca
    17281 gaacaaccgg aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag
    17341 acattcccac aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg
    17401 atctttgcga ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa
    17461 caactaaata ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt
    17521 tttgtatcca aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc
    17581 cagatatggc cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg
    17641 aaggatatgg gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc
    17701 aacgagccga tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc
    17761 aagatcaacc actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
    17821 atcgtgggtt taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa
    17881 agaagatgag attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac
    17941 gattttccgc attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg
    18001 aggtaaaaga acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta
    18061 gtatcccagc aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca
    18121 atccaatcat ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt
    18181 tcattaaggc cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc
    18241 aaaatgaagg aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt
    18301 gtctcgatca atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct
    18361 atgatgaaaa aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgtcg
    18421 aagaagattt tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg
    18481 aagtaaatga aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa
    18541 tgccggatgc cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag
    18601 cagtaaatga ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt
    18661 cgattggagg tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag
    18721 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
    18781 gtcttttatt ttcaagagct tagacaattt gatatttcta agctatcact ttctactata
    18841 gaaatcgttc gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga
    18901 agcacgatgt tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga
    18961 caaaagctag cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt
    19021 gaaggatttt tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc
    19081 aaaatctatt accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga
    19141 aaatatcaag atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa
    19201 ctagtagaaa aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt
    19261 gccaatcgaa ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa
    19321 aatcaagtat gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt
    19381 gggaagactt actttttact cactatcatc gaggctttat taaaatctga tgccgagctt
    19441 tttgttcttg atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc
    19501 tattcccaaa aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact
    19561 cgtagcaagg caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt
    19621 ggacttccac caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg
    19681 acgaaggaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa
    19741 tctggtttct ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg
    19801 attcgcgatc aatttaattt ccgggtggct ttgggtcgta tgagtgaact gggatattcc
    19861 atgatgtttg gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat
    19921 gtagatacgg gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat
    19981 gatttcttaa gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat
    20041 aatagtatgt agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga
    20101 catttttaga tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc
    20161 accagtgtat cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc
    20221 atgatacaag taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc
    20281 aatctctcgg accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg
    20341 cgggaaagcg gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    20401 tcgccgctat tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc
    20461 acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg
    20521 tggaatggca agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc
    20581 ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc
    20641 caacatctga tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg
    20701 ggggacaagt ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca
    20761 aaatcgcatt gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg
    20821 aacaaggcaa tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag
    20881 gcggaacgaa gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga
    20941 acagtgtgaa cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa
    21001 gtgtgcatac cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc
    21061 gaaagttaac acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc
    21121 gagcttctca agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag
    21181 ttccggtcat ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat
    21241 gagttgattg gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag
    21301 aagaactgta ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc
    21361 cgtatgtaat tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat
    21421 tggaacgaat tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa
    21481 aagcagagtt ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag
    21541 gcactgaccg cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg
    21601 acggcgttag ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac
    21661 aaaaaataag caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt
    21721 gtagatgtta actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac
    21781 aaaggaattt agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa
    21841 ataaattagc tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg
    21901 gcgtaaccgc atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc
    21961 ccgagttacc tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg
    22021 cgaggaaaat cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt
    22081 acgcctttta ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac
    22141 atgaagaaga taaaggcgtc cttttagaat taaagggtcg tggttgtcgg cagtttgaaa
    22201 cttttttact cgcccaaaag cgtagctggt atgacttttt cgaagattgt ctaaaagccg
    22261 gtggcgtgat gaaacgctta gacctggcaa tcaatgatcg agttggcctg ttagatattc
    22321 ctgatttaac gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa
    22381 gctatcgttc cggggaactg ttgaaacctg atgataaaga tggtatgggc aatactttgt
    22441 atattggcag tcttaagagt gaggtctatt tctgtttgta cgaaaaagac tatgagcaat
    22501 atatcaagtt agggattcct ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac
    22561 tgaaaaatga tcgagcttat catgcaattc aagatttgtt aaaaggtcgc agtattgaaa
    22621 gtaccacatt ttccattatt aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa
    22681 ggcgaactaa ctggcccttg aatgaacaat gggggcgctt cattggacgg aatcagaagg
    22741 aaattcaatt aacttctgaa ccgaagcctt ataccattga acgaacgttg aactggcttg
    22801 gacgacaagt cgcccctacg tggaagatgg ccaaggagtt ggatcggttg aatcagacaa
    22861 cgtacattca ggatatggta aataatactc gactatcaga ccggcacaaa aagattttgg
    22921 agcaacaaag tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tgatggaaat
    22981 tgtgaaagat attttattgg tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt
    23041 gatgcatgca agtgcagcgg cggaccgagc aatggaaaag aaaggaaagt gatgaaatga
    23101 atttcggtca aaattt
//
LOCUS       DASJST010000035        22769 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJST010000035 DASJST010000000
VERSION     DASJST010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.035"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            58..468
                     /locus_tag="U4B05_001088"
     CDS             58..468
                     /locus_tag="U4B05_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN2050240.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            461..1162
                     /locus_tag="U4B05_001089"
     CDS             461..1162
                     /locus_tag="U4B05_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN2050241.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1162..2748
                     /locus_tag="U4B05_001090"
     CDS             1162..2748
                     /locus_tag="U4B05_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050242.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2767..2988
                     /locus_tag="U4B05_001091"
     CDS             2767..2988
                     /locus_tag="U4B05_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN2050243.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2985..3746
                     /locus_tag="U4B05_001092"
     CDS             2985..3746
                     /locus_tag="U4B05_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN2050244.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4126..4635
                     /locus_tag="U4B05_001093"
     CDS             4126..4635
                     /locus_tag="U4B05_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2050245.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4710..5249)
                     /locus_tag="U4B05_001094"
     CDS             complement(4710..5249)
                     /locus_tag="U4B05_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN2050246.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5389..6000)
                     /gene="rpsD"
                     /locus_tag="U4B05_001095"
     CDS             complement(5389..6000)
                     /gene="rpsD"
                     /locus_tag="U4B05_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN2050247.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6262..7113
                     /locus_tag="U4B05_001096"
     CDS             6262..7113
                     /locus_tag="U4B05_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN2050248.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7141..7776
                     /locus_tag="U4B05_001097"
     CDS             7141..7776
                     /locus_tag="U4B05_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN2050249.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7796..8569
                     /locus_tag="U4B05_001098"
     CDS             7796..8569
                     /locus_tag="U4B05_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2050250.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8977..9774
                     /locus_tag="U4B05_001099"
     CDS             8977..9774
                     /locus_tag="U4B05_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN2050251.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9752..10165
                     /locus_tag="U4B05_001100"
     CDS             9752..10165
                     /locus_tag="U4B05_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2050252.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10149..12794
                     /gene="mutS"
                     /locus_tag="U4B05_001101"
     CDS             10149..12794
                     /gene="mutS"
                     /locus_tag="U4B05_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2050253.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12812..14920
                     /gene="mutL"
                     /locus_tag="U4B05_001102"
     CDS             12812..14920
                     /gene="mutL"
                     /locus_tag="U4B05_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN2050254.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14942..15502
                     /gene="maf"
                     /locus_tag="U4B05_001103"
     CDS             14942..15502
                     /gene="maf"
                     /locus_tag="U4B05_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN2050255.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15587..17575)
                     /gene="treP"
                     /locus_tag="U4B05_001104"
     CDS             complement(15587..17575)
                     /gene="treP"
                     /locus_tag="U4B05_001104"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN2050256.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17798..18511
                     /gene="treR"
                     /locus_tag="U4B05_001105"
     CDS             17798..18511
                     /gene="treR"
                     /locus_tag="U4B05_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN2050257.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18588..19034
                     /gene="msrB"
                     /locus_tag="U4B05_001106"
     CDS             18588..19034
                     /gene="msrB"
                     /locus_tag="U4B05_001106"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN2050258.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19204..19806
                     /gene="ruvA"
                     /locus_tag="U4B05_001107"
     CDS             19204..19806
                     /gene="ruvA"
                     /locus_tag="U4B05_001107"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN2050259.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19819..20820
                     /gene="ruvB"
                     /locus_tag="U4B05_001108"
     CDS             19819..20820
                     /gene="ruvB"
                     /locus_tag="U4B05_001108"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN2050260.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20849..21427
                     /locus_tag="U4B05_001109"
     CDS             20849..21427
                     /locus_tag="U4B05_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2050261.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21479..21961
                     /locus_tag="U4B05_001110"
     CDS             21479..21961
                     /locus_tag="U4B05_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN2050262.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22078..22452
                     /locus_tag="U4B05_001111"
     CDS             22078..22452
                     /locus_tag="U4B05_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050263.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7409 a         4072 c         4927 g         6361 t
ORIGIN      
        1 aactttcaga aaaggtttgt tagtaaaatg aaagctatac gtaaggagtg ttgtttgatg
       61 aaattttttc aagagttttt cattattatt ttcttttctg tattaggaga aggcatacgg
      121 atttttgctc atttacctat tcctggaagt atcttaggta ttcttttttt atttttagca
      181 tttgaaatgc atcttgttga tcctgaaaaa atcggagcaa ctggtgattt tttattgaat
      241 aatctagcca ttttatttgt tcctgctggt gtaggtttat tggaatattt tgatgatatt
      301 gcttcgatat ggcctgtttt actgggagca gtcgtcgtgt gttccatcgt cacgatggtt
      361 gcagcgggaa aaacggcaga aggtgtagaa gctttgctgg gctatgtccg taagaaaaaa
      421 gcagaaaggg tatctgtaaa cgaaatggag gagaatcaaa ttgactgagt tttttgtgaa
      481 tccattattt ggcttaagtt tgactttagg agtgtacgta ttatctgaca aatttttgcg
      541 gaaatataaa ctgcctgttt tgaatcctct cgtattatcg attgttgtta ttatcttatt
      601 tttgcatttt tctgggatca gctataaaga ctataatatc gggggcaatt ttttgactat
      661 gctgattact cctgcgacag tggcacttgc catccctctt tataaaaact tccgtttact
      721 gaaagagaat ttttatcctg taatggctgc gattttagca ggaatagtcg caaattgctt
      781 ggttagtgta gggttcggtt acttcttctc tcttcataaa gagatggtga tctcattact
      841 acctaaatct gtaacgacgg ctatttctgt cgatttatct catacgatgg gcggggtgaa
      901 tgccgtgaca cttgctattg ttgtctctac aggtattttt ggttcgctga tcgccactca
      961 tattttccgc ctattcaaga tcgaaagtcc agttgctcgc ggtgtggcgt taggaagtac
     1021 ttctcatgct attggtacag caaaagcaat cgagattggt gaaatagaag gaatcatttc
     1081 cggacttgcg atatgcgtca acggaatatt gaccgtttta ttgctaccgt tattctttca
     1141 gccttttgct ggcttgtttt gatgcgaaaa ccactatttt atgctacaat aaccaaaacg
     1201 ttcgagcata aaaaaggagg gaaagacgtg tatttgtctt ccgagtatct taaacaattt
     1261 gataaagatc tatattataa gattctcctc ctagaaagtt ttgaggatca agcttggcat
     1321 accgctgcac aattagcgca agttgttcag ctggatgccc gcagcgtgtc gaaatacctg
     1381 aacgagctgt ctaaaaacta tcagcagttt agcggtaaga cacacccttt gtttacaaaa
     1441 aatcaccgtt ctggttataa cttttatgat acgctcgatt caattgaaca tgagcgtttt
     1501 ttgatctatc tcgtgcaaag tacgctgaaa ttccagttgc tgcacgacat tttttttgaa
     1561 gaattccata caatgtacca atttgcgcaa aagcattaca ttagtgaatc tactgctcat
     1621 cgaaaaatca atgaatggaa acagcaactg caaacttacg gaattcggct gcaacgaggt
     1681 acatatatcg ctcaaggcga agaagaaatc attcgacttt acttgcatat gacattctgg
     1741 cagttgtttc gagggaaaat ctggccattt gaaacgattt ctcaaatgga cgtaaagaat
     1801 atggcagaac atatcatggc tttttttaat gttcgtctga acgagatcaa aaaaagacga
     1861 ttggaatata tgctaggtgc ctttttttta agaaaatcac agaagcatta tgttgtattg
     1921 aatgagaaaa aaagacggct gatttctgac aatctgttgt tccagcgatt ttgccaggtc
     1981 atggagccgg tatttcctaa ttattttcaa gtagaagacg agttaggcgc attatttctt
     2041 gtactgatga ctcgtgaaga atattatagt gatcctaaga tacggaagaa aatttttgat
     2101 tttcatcaac aagcaaaaac accgcccttt acagcccttt cagaagcaaa agccgctctt
     2161 tctctttatc aggaagagca ggggctgcca gcagaaaatc taaccttcga ggcagaaaac
     2221 tatttattca gcagtcattt tttcgcctat ctttttccta atgcgaaaga aacgattgat
     2281 ggcaatagta gtgattttat taatcattta gttattgaaa acaaggaatt gaaacaatgg
     2341 ctcgtacatt tttttgaaag tagacacaaa catcccaatc atctggcatt taaaaatcat
     2401 gcgtttttga tggggcggta tctgactgtg tttaaaacat taggagcctt tactccacag
     2461 cttccaaaga tcacgatcct tttaatgaca gatttcccat tatttgaaga gcaactgtta
     2521 gaggagggat tacggacatt tttccgtaat gagtatcagc tgatttttct gccaacagat
     2581 tatcggggga gagaagttga tcttctgatt tcgacaagta aagtccaccg caaaccatgg
     2641 gcggatcttg actattttat tgtgactgaa gaactaaaat taattgatta catccaacta
     2701 tctcaaaaat ttgaaatgat tcaaaaacaa aaactattaa aacaataaca aagaaggaga
     2761 aaaaagatga catttgaaga aattttacca gaattgaaaa aaggtgcaaa gattattcgt
     2821 aaaggatgga gtggatttga actttatgtt gttctcgtat ccgatgatga atttgatggc
     2881 tgtccggtga cgccgtactt attgatcaaa acaagtgacg aaggtttttc cagcttcgct
     2941 cctacagttt gtgatatttt agcagatgat tgggcgatcg tagaatgaaa aaccagtatc
     3001 cagatctagc taaacaggtt gtatttatca caggagctgc ttctggaatt ggtgctgcgc
     3061 aagcccgagc gtttcttgaa caagatgctt ttgtatttgg attagatcaa aagttcggta
     3121 aaatggaaga aatttctttg cgatttcctg aaacttttgc ctacgcacta ggtgatgttc
     3181 gacaaatgaa agaactccaa caagcggtat cgaaatgcca aaatcatttt ggtgaagtta
     3241 cgattttact gaatacagcg gggatattgg atgcctacaa acccttgatg gaaactgacg
     3301 aatcgctgtg ggatctgatt tatgagacga atgtgaaaag catgtatcag ctgacaaaaa
     3361 tcatccttcc tgatatgatc cgccaaaaaa gcggaacgat catcaatatg gcttcgattg
     3421 ctggcttgat tgctggtgga ggtgggatcg cttatacaag cgcgaaacat gcgattgtcg
     3481 gatttaccaa acaactcgct ctggattacg caggacaggg tatccgagta aaaggaattg
     3541 ctccaggagc catccaaaca ccaatgaatg cggctgattt tgaagggaaa ggtgagatgg
     3601 cagagtgggt ggcaaatgaa acaccagtca agcgatgggc acagccggaa gaagtagctg
     3661 agctgacgtt atttcttgcc tctcctcaag caagctacat acaaggaacg atcatcccaa
     3721 tcgacggcgg atggttgtta aaataacctt gtgttttttt caagcttctg tatgataaaa
     3781 ctaaagagag aaacaacggt tgatgataaa aaaagtcgat gactgttccc gacattcgat
     3841 gccgaacagc cagttcaaaa aattcttttt aataatcgtt aatattcgaa actaagcggc
     3901 ttttcacgac ctcttgatat aaacgatatt caaaagcttg caactgcggt aacaagtcca
     3961 gccaaatgaa aaatataagg agctattttt atttgaccat tcttattact tgttgcaggg
     4021 cagctttttc actaccttct gcgacacttc agttggaatt ttcttcccat cctttggtcg
     4081 cggtcattac caagataaaa ttatcttgca aaggagaaat agaacatgga aaaaaataat
     4141 acccgttcag ccaaacgctg gacaacgaca gatgtgactc ggatggcgct agtcactggt
     4201 ttatatgtgg cagtgacgct ggttttgtct gtctttagct ttggagtgat tcagattcgt
     4261 ctctcggaga tgttcaacta tctgtccttg tataacaaaa gatacatcgt cgcggtaaca
     4321 gcgggagtag ctttggctaa tattgcttct cctcttggac tgatagatgt tgcagtagga
     4381 agcgtcagca cgctgcttgt attgcttatc aactataaaa tcactagtcg catcaaaaac
     4441 atgaaaatca aaatggccgt gacagcatgc gtatttgctt tttccatgtt tacagtagct
     4501 ggtcagctaa cgattttata tcaactacct tttttcttga attggtgggt gattgcttta
     4561 ggagaattgg catcaatgat tgtcggtggg ataattattt attggatcgg taaaaaagtg
     4621 gatttgtcaa aataaagatc tgtttaattt aaaatagggc agcatggaag agactgaatg
     4681 ttgagtcttc ttccatgctg cttttttgat taatttgtat gaagaaatcg atttagccgt
     4741 tttttcatca agataccgat ataagctgct ccagttgcct tgattgcttc cggcagtaaa
     4801 aacggcagta gtccactggc aaatgcagtt gtaaaagtaa gatcaccact tattttcagc
     4861 cagatcgttc cgcagatcaa tgcagcaaga gagcccatta gattggcaaa gattgcatat
     4921 aagtaatgag catccgttct ttcgatcatc caaccagtta tccacgtttg taagagaaat
     4981 ccaatcaaat atccgccagt tggcccaaat aaaacagcta tacctccagt catccctgaa
     5041 aatacaggaa gaccaatcaa gccaagtaaa agataaactc caacagatat caatcctagt
     5101 tttcttccga gaaaagttgc tgttaatcca atagcaaatg tttgccctgt caaaggaacc
     5161 aaaccaaaag gaatggtcat ctgagaaagg acagaaataa tcgcggcaaa ttccgcagca
     5221 agtatcaatt ctttcaatgt tcggttcata acgccctctt ttcgttaact taatattttt
     5281 atttggttaa cgaaattgta aaacaaagtg attactttgt aaatagaaaa tataaaaaaa
     5341 gaccagctat ccaatagata gcggtctttt tcaagaaagc ttttgaaatt ataagctttg
     5401 gttgtagtat tcaacgacaa gagcttcgtc gatttctgga tatagttcgt cacgttctgg
     5461 taaacgagtc aatgaacctt ccaatttttc agcgtcaaag cttacgaatg ctgggcgtcc
     5521 aacagttgct tctacagctt cttttactgt tacgatgttt tgtgattttt cacgaactga
     5581 gatcacttga cccacttcta cgtggtatga agggatatca acgcgtttgc catcaactaa
     5641 aatgtgacca tggttaacta attgacgtgc ttgacgacga gtcgttgcaa gtcctaaacg
     5701 gtaaacaaca ttgtctaaac gttgttctag taagatcatg aagttaacac catgtttacc
     5761 ttctttgatt ttgctagctt ttacgaacaa gttacggaat tgacgttcgt tcattccgta
     5821 catgtgacga agtttttgtt tttcagttaa ttgcatgccg tattctgatt gtttgccacg
     5881 gctattaggt ccatgttgac ctggtgcgta agggcgacgt gctaattctt ttcctgtgcc
     5941 tgacaatgag atgcctaagc gacgagaaat tttccatgat ggtcctgtat aacgagacat
     6001 taaaatttcc tccaatgaat ataagttttt ggagtaaaat aatccgttga aaaattcata
     6061 atcatatagt ttgttcttca atcttcacct ttgcagccgc ggttacacaa tcgaacccga
     6121 gacggggcaa caaactaggg atgagttatt atttttctgc tgcattattt tacacaaatt
     6181 acagtatatg atagatcatc cacgaatgtc aagataatta gtacataaaa acgctttaca
     6241 aacctaggct gtctttataa aatgagaggg tatgtgcaaa tggaaaaaag cgaaggaagt
     6301 ttaaggagaa aatcaatgaa tccggtatca ccattatttg agaaaatcga tgcttcaatt
     6361 gaaaagaagg cgaatctttt tgaaaacagc ttttcacgtt acgctgttcg agcggttctc
     6421 gcctgtttgt ttttgacttt aggaacggct gtggcatttg cgatcgcgat gaagggggaa
     6481 gatatcgcgc atggtctagg aaagatttta tacgcattta tgttcagctg gggtctggtc
     6541 atgattctat acatgaacgc cgaattagga acatcgaata tgctttatat gaccgtgggt
     6601 gtttttcggc agagacttgc tttaagtaag gcagccaaga ttctatttgc ctgtattttt
     6661 ttcaatatga ttggcggtat attatgtgga tttctaattt ctatgactac gccatttcaa
     6721 gatttaacgt tggacaactt tatgttagaa tcaatagcag taaaactagc aaaaccttca
     6781 gcaacgattt tggtggaagg gatgtttgcg aatatcgttg tcaataccgc tgtactgatc
     6841 agcatgcgga tgaaagagga tgtcggaaaa gtgatcacag tcgtattcat cattttcatt
     6901 tttgcttttt taggatatga acacgtgata gcgaattttc ctgcctttac gcttacttac
     6961 tttgcttctc acggacagat ggatggaatg acagtcagca atgtgttaca taatttgttt
     7021 tttgctttgg caggaaatta tattggtggc ggtttagtaa tgggactggg atatgcttgg
     7081 ctgaatcagt caaaatcatc ctatgtggac taaaaagtcg ttcattaaag gggaagtgcc
     7141 atggaagttt tagtttttcc aaaaaatgag gaaaaagcgt tacaaatgga aaagtacatg
     7201 aaaaatcttt tttcctttgc tggagtacct aagccggaac gcgctctttt acaacaaccg
     7261 ttactggatg agagcaaaaa tttatctatt tctgaagttt tagcttttgt tcattattat
     7321 tatgaacaaa tcgaaagaga gtatcagtat gtagcgatcg atttagtcca gaaaaacatc
     7381 aaacggctta catttgaaga cttagtcgta ttgttgccac tagttcagaa aaaagaatgg
     7441 tgggacagca tcgactcttg gagaaaagta tacagcacat ggtgtaaagc ctacccagag
     7501 cagcgtgaag aagtctttca attgtttgaa aaaaaggaag atttttggct acgtcgaata
     7561 gcaatcacgt tgcaattagg ttataaacag caaacagaca caggactatt gatccgagca
     7621 atagacgaag atcgtcaaac aaaggaattc tttatccaaa aagcaattgg ttgggcatta
     7681 cgcgattata gtaagaccga tgccgattgg gtaaaaagac agctgacaac agatttgagt
     7741 aagttagctg ttcgagaggc aagtaagtat ttataagaaa gcgagacgac aagagatgag
     7801 acgcaaatta tttgcttttg atattgatgg aacgttgtta gattcaaaca aacaagcttt
     7861 agatagcaca agagaagcat tggaaaaatt acgcaaagcc ggtcatctgg tcacgattgc
     7921 tacagggcgg agccgatttc atgcgcaaga gatcattcgc gatcttgatt ttacgaacta
     7981 cattttatgt aatggagcgg ccggtttttt agaccatgaa caagtatata agaatttact
     8041 agatcgcgac cagttggatg cgttcgttca agaagcgcat catgagcaga tcgatacagc
     8101 ttttgtcagc ttagacaata tcaagcgttc ttcttccaat gatgtaacgg tgatggaaga
     8161 agcgatgcat tctttcggtg ctgtcttgcc tgaattagat cagttttttg cagaggaaca
     8221 agaaatatac caagcgttag catttttcga tcaaacatat gatgggaagt tctcttcata
     8281 tgataaaatt cgatttgtcc gctggcatga gaacagtgta gatgtcgtac ctaaaaatgg
     8341 ttctaaagca gcgacaattc tgcacttagc agaacaagta ggcatccctc atgaagacat
     8401 catcagcttt ggcgatggac aaaacgaccg tgaaatgttg cgcatgtcag gaattgggat
     8461 agctatgggg aatgctgtgc cagaagtaca agcagaagct aagatggtca ctgatacaaa
     8521 cgatcaggat ggaatttgga aggcattgaa agagttaagc gtgatttaaa agaggttgtg
     8581 aaaaagctgt tctgcatcaa gtaataagcc cagccaaaca aaaatagctc ctcctatttt
     8641 tcgtttttct gggcttatta ccgcagatgc aagctttcga acaccgttta aggaagaggt
     8701 cgtgacagaa gtggtcagtt tcaagaaata agaagggatt tacgaaaatt gcttctcaaa
     8761 tttttgtaaa attacggctt atttctgaag aagtagatga aacaacgttt attcgggaaa
     8821 tgggactgtg acaagatgtt tgtcatagtc ccattttttt attttatttt tcaaaactaa
     8881 gaaataccag gaaagagttc cttttgtttg accttttctt tcttattgaa aacaactata
     8941 atatagagtg atgttaaaag agaagaggaa tttagtgtgc gggtattgtt tattggtgat
     9001 gtagtaggtt caatgggacg agagatgatt actgaatatc ttcctagatt gaagaagaag
     9061 taccgtccgc aagtaacgat tgtaaatggt gagaatgccg cttctggacg tggaatcaca
     9121 gaaaaaatct ataaaaaatt tttgcaagac ggcgtagatg tcgtgacgat ggggaatcat
     9181 acgtgggaca accgcggaat ctttgagttt gtaaatgaag ctaaaaagat ggttcgtccg
     9241 gcaaattttc cggaagggac gcctggacaa gggatggtct ttgtcaaagt gaaccaagtc
     9301 gaactggcag tgatcaacat gcaggctcgt tcttttatgg tggacttgga tgatcctttt
     9361 cgtaaaatga atgagctggt agaagaagcc agaaaacgta cgccgatcat ttttgtagat
     9421 tttcatggag aaacaacaag tgaaaaacaa gcaatgggct ggtttttaga tgggaaagtt
     9481 tcagcagtgg tcggtacaca tacacacgtt cagacaaatg atgcgcgaat tttgccaaga
     9541 ggaacagcgt atctgacaga cgttgggatg acaggtccct atgatggtat tttgggtatg
     9601 cgcagagaac ctgtaatcga aaagtttctg actgcattac caaaacgttt tgaagttgtg
     9661 gaacaaggcc gatcgatttt aagcggctgt atcttggaat tggatgatac gacaggtcat
     9721 gcaaaagaaa tccagctgat tcagatcaac gatgatcgac cgttcatgga gtagaaagag
     9781 aggcagtttt ttgcagcagg agccggaaat tcaaaaagaa gtaaggaaac tgacaaaact
     9841 tttaagagag aatgaaacga tcattcgcta caaagaactt gaagaaaaga tccagcaaaa
     9901 ccagtattta gctgagttga gagaaaaaat caaacaagcg caaaaagatg ctgttcattt
     9961 tgcccactat gacaaaccag cagcagaaaa agaagcaatc aaacaagcgg atcaattcat
    10021 gcaagaattt gatcagcatc cgcttgttgt agcttataga aaacagctgt tggaagcaga
    10081 tgatctgctt catcatttaa caactatgat acaagaagaa atcaatggac aaatagagga
    10141 ggaaaaacat gcctcaaaaa actaaaaata ctcctatgat ggaacaatat ctaagcatca
    10201 aggcccaata ccaagatgct tttttgttct atcgtttagg agacttttat gaactatttt
    10261 acgaagatgc gatcaaagcg tcacagcttt tggaattaac gttgacgagc agaaatcgaa
    10321 atgcagaaga acctattcct atgtgcggtg tacctcacca tgcagcacaa ggatatatcg
    10381 atgccttgat tgaaaaaggc tataaagtag cgatttgcga gcaggtagaa gatccaaaga
    10441 cgactaaagg gatggtcaag cgcgaagtga tccagctagt cactcctggt acagtcatgg
    10501 acagcaaagg attgtcggca aaagacaaca attttttaac agctgtagtt agtcaaggaa
    10561 atgaatttgg ctttgcttat gccgatttaa gtacgggaga attgaagacg gctcggttgc
    10621 atgatgaaga agctgtttta aatgaagcct ctgcattgca gacaaaagaa ctcgtattag
    10681 gcagtgagat cactgatacc cttcgtgaac agctaagcgg gcgtttaggg ctagtctttt
    10741 ctcaacaaaa tgagatggaa gaaaacgcgg aatttagctt tttgacaagc gaactagtcg
    10801 atccgctgga aaaagaagca accggcaaac tattgactta tttatcggtg acacaaaagc
    10861 ggagtttagc tcatatccaa aaagcagtag aataccaacc agatcatttt ttgaaaatgg
    10921 attattattc aaaattcaac ctagaattaa gccaatctat tcggactggg aaaaagcacg
    10981 gcactcttct atggttgtta gatgaaacaa aaacagcaat gggtggccgt ttactaaaac
    11041 aatggctaga ccgtccgctt atccaggcaa aacaaattaa agcaagacaa gaaatggtcg
    11101 cttctttgct agacagctat tttgaacgta tcgatttgca gtcagcatta acgaaagtct
    11161 atgatctgga acgcctagca ggtcgagtag ctttcggtaa tgtcaatggg cgagacttga
    11221 tccagctgaa aacttcttta gaacaagtgc caatgatcag agggttgatt gaaggaatcg
    11281 atcaaggtca atggcaaagt ctgctttcag aaatgcagcc gatggaccat ctcgtccaat
    11341 tgatcgacca agctatccaa gatgatccgc cgttacaaat aacggaagga aacatcatca
    11401 aagatggatt caatgagcag ctcgatatgt accgttctgc tatgagaaac ggaaagaaat
    11461 ggttggcaga acttgaagca aaagagcgtc aagaaacagg aatcaagaac ttaaaaatcg
    11521 gatttaaccg cgttttcggt tattatatag aaattacaaa agcaaatctt ggaaatgcag
    11581 aactggagaa gtatgaacga aaacaaactt tagcaaatgc agagcgtttc atcacaccag
    11641 aactaaaaga attggagaca caaatcctcg aagcagaaga aaaatctgtt gatttagaat
    11701 atcagttgtt tttagctgtg agagaagaag tcaaaaaggc tatccagccg ctacaagtac
    11761 ttgcaaaagc aatcagtgca gtagatgtgc tacaaagttt tgcaacgatc agcgaacgct
    11821 atcagtatgt tcggccagaa cttgtcagtg acaagcatca gcttctgatc aaagacggac
    11881 gacatcctgt agtagaaaaa gtattaggac atcaggaata tatccctaac agcgtagaaa
    11941 tggcagaaga cgaaatgatc ttgctgatta caggaccgaa tatgtctggt aaaagtacgt
    12001 atatgcgaca gttggcatta actgttctga tggcgcaaat gggctgtttc gttccggcaa
    12061 aacaagcagt tttacctatt tttgatcgga tttttacccg aattggtgca agtgacgact
    12121 taattgctgg acaaagtact tttatggtag agatgatgga agccaatcaa gccctgcgtt
    12181 atgctacgcc aaacagtttg attttgtttg acgaactagg gcgaggaaca gcaacctatg
    12241 acggaatggc gttggcccaa gcaatcatcg aatatatcca tcgtcatgta caagcaaaaa
    12301 cactcttttc tactcattat catgaactga cggtacttga gaaagaactt ccgcagctta
    12361 aaaatgtcca tgttggagcc gttgaaaaag acggagaagt tgtcttcttg cataaaatga
    12421 tggatggacc tgcggataaa agctacggca tacatgtggc aaagatcgca ggattgcctt
    12481 ccaatctact agaacgagca gcagatatcc tgcatacgct agaaaatggc gataacggac
    12541 accatactgt tccagctgaa gtagaagaga aaactaaaac agccaaacag gagcaaataa
    12601 aaccacctgc agaaaaagta aatcaagacc atatacaaga ccgtgtaaaa gaagaaaacc
    12661 aacagctttc tttattcagt gaattatccg aaaatgaaac agaagtcatt gaatcactga
    12721 agcaaataaa tttactagaa atgacgccaa tggatgcact aaacacatta tatgaattac
    12781 aaaaacaact gtaggaagaa ggcggatatc gatggccaaa atccaagaat tatctgaacg
    12841 tttagctaat cagatcgctg cgggagaagt agtggagcga ccagcttccg ttgtgaaaga
    12901 attagtggaa aatgcaattg acgccggcag tactcagatt gatatcttag ttgaagaagc
    12961 gggactgcgg acgatccaag tgattgataa cggagaaggc attttggcag acgacgtaga
    13021 gaatgccttc aagcggcatg cgacaagtaa gatccataat cgcgacgatc tttttcgcat
    13081 acgaacgctg ggctttcgag gggaagcttt gccaagtatt gcctctgttt ctgaaatgac
    13141 tctggaaacg gcgaatcagg aagaaaaaca aggcacctat ctgtcgctga aaggcggaga
    13201 agttattgag catcgaccag caccactgcg tcaaggaacg aagattaccg tcagcaatct
    13261 gtttttcaat acgccagccc gcttgaaata cgtgaaaaca ctgcacacag aattagcgaa
    13321 catcggggac atcgtcaatc gactggcatt gagtcatcca tcgattgctt ttcgtttagt
    13381 acatgatgga aacaaaatgc tggcaactgc tggaaatggc gatctgaaac aaacaattgc
    13441 gggaatctac ggattatcta ccgcaaaaaa aatgctgaaa atcgaaggaa aagatctaga
    13501 ttttgagtta tttggctatg tttcattgcc tgaggtgact cgtgccagta ggaattactt
    13561 atcgacgatc atcaatggac gttttatcaa gaacttttcc ttgaataaag caatcgttgc
    13621 aggttatggg tcaaaattaa tggtcggtcg tttccctatt gctgtattgg aaatcaaaat
    13681 ggacccgctt ttagtagatg taaatgttca tccgacgaag caggaagtcc ggctgtcgaa
    13741 ggaaaaagaa ctaacagagc taattagtaa cgccattcaa aaagcattgt ctcaagaaaa
    13801 tctgattcca agtgcagcgg ataatctacg gtttaagaaa aaagtacctg atcagcctaa
    13861 aacagaacaa atcgaaatcg atttgtccta cgcttttgaa gaagaaaaac ctaaaaagcc
    13921 aagtagtttg aattttgatc gacagacagg tacctttttc gtagaagaac taaagaacac
    13981 atttgttgac agaattgttg ataagtctgt ggaaaatgtt gaaaactatt ctggtttttc
    14041 tgaagaaaac accacttatc cagaaaaaac agcgaaaacg gtggataagt ttggtgaaaa
    14101 tgttgataac tttgtagaaa aaacagtgga aaaatctgaa aagaaagaat ttcccattgt
    14161 ggataacatg gagaaaacaa atgaagaaca agcagataaa tccgtagaag aggatgaaaa
    14221 gatttacgag gaagaattga cactccatcc tgaatttgat ttcactcaag gaaacgacaa
    14281 aacattagaa caagccatca agaaattaga aacggaaaaa ccagtacaac gttttcctca
    14341 gttggaatat tttggacaga tgcacggaac gtatcttttt gcccaaagta gcgatgggtt
    14401 gtacatcatt gatcagcatg ctgcccaaga gcgcattaag tatgaatatt tccgggaaaa
    14461 aattggggaa gtatctgatg atctgcaaga attgcttgtt ccatttgtgt tagactatcc
    14521 aaacagtgat gctttgaaat taaaagagca aaaacacttg ctagaagatg cagggattta
    14581 tttagaagaa tttggtcaaa acagctttat tgtacgggcc catcctactt ggtatcccaa
    14641 tggagaagaa gaatcgatta ttcgggagat gatcgatatg ctgctgacaa ctggaagtgt
    14701 gagtgtaaaa aaattccgtg aagcaacagc aatcatgatg agttgcaaac gatcgattaa
    14761 agccaaccat tatttgaatg aagcacaagc acgtgtgctg ctgaaggatc tggcacgatg
    14821 cgaaaatcct ttcaattgtc cccatggccg tccagtattg attcacttta ccaattcgga
    14881 tatggaaagg atgttcaaac gaatccaaga cccgcattaa gtcaaagaga agagggaaaa
    14941 catgaagatt gtattagcat cccaatcacc aagaagaaaa gagttattgg ctcatatcgt
    15001 ttcagaattc gagattgaac ctgctgatat cgatgaaacg ccatttccag acgaacagcc
    15061 cgtcgattat gttcgtagaa tggctgaaga aaaagcacga actgtctggg aacaagatga
    15121 aaaagaagac catttagtga ttgccagtga cacgactgtt gttctcaatc aagagatcat
    15181 gggtaaacca gaagatctgg ctgaggctga attcatgctg aaaaaactaa gcggggaaac
    15241 acatactgta tatacagctg ttgttttaaa aacaaaggaa cgagaagaac ggatactagc
    15301 agaagcacat gtcacttttt atcctttgac agacgaagag atcaaacgct acttggagac
    15361 gggggattat gcggataagg caggggcata cggcatccaa aatgctgcga gtgtattcgt
    15421 caaagaaatt tctggagatt attacagtat cgtaggattt ccgattggtg ctgtcaatca
    15481 ggcactgaaa acattcattt aaacaaaaaa gcggactatc tgattttcga ttgaaaatcc
    15541 atgatagccc gtttttttta ttgaaaagat tacttatcta ctctgactaa gattttactt
    15601 cagctttacc gactgcttcg cccgggcgaa caagtcctga ttggtcaaga gaaagtcctg
    15661 caactttttc attattggtc aaagcaacaa taatgtcagt tttcttgcca gcttgttcta
    15721 tttctggcaa atccatttca gcaatcaggg tctctggtgt gacaaggtat ccttctttca
    15781 cgaatacatt gaaaggaaca ccctttagtt caacagtatc taatcccatg tgaacaagta
    15841 cttccagccc ctcattagaa agaataccaa tggcatgttt tgtttcaaag acactcgtca
    15901 ctttaccgtt tactggtgca tagactttgc cgtttgacgg ttcgacagca taaccgtcac
    15961 ccatcatttt ttgtgagaaa actgggtcat ttacttcagt tatttccttg atggtccctg
    16021 cagcgactgc gaataatgtt tcttttgttg caactgccgt accagttgct gaaacaggtt
    16081 cgaaagactg tggcgttgct gttgcgccat ttgctgtttg aagttgtaca tcagcagcat
    16141 tttcaggaac agcaggatca atcttattca aaattccctg acgtctaaag atgatcgtca
    16201 agccaaatgg aatgatgaca gcaatgatca ttgcgatgat gaaaggtacc catgtttctg
    16261 cttggatagc tagtattcct ggcaaaccgc ctactccgat cgcgttagct cgaacaccca
    16321 tcaagttagc aaacatgcca gctaatccag agccgaccat tgcagcgacg aaaggataga
    16381 cgtatttcaa attgattccg aacatagcag gttcagttac gcctaagtaa caagaaatca
    16441 ttgctggaat cgagatttgt tcttcttttt tattcccacg atgtaagaag atgatggcta
    16501 agactgcaga gccttgggcg atatttgata aagcaatcat cggccataaa ttcgttgatc
    16561 cgaaatctgc aatcagctgc atatcgatgg cattggtcat atggtggaga ccagtaatca
    16621 ctaaaggagc atacaggaat ccgaatacgg cactaaacaa ccagctgata gaagacgtca
    16681 atccagtatt tacgatattt gagatccagc ttccgactgt ccagccgata ggacctaaaa
    16741 taacgtgtgc agctaaaaca gttggtaaca atgaaaacaa tggaacaaaa atcatcgaga
    16801 tcgactgagg gatatatttg cggaagaaga tttctagata cgctaacata aaccctgcca
    16861 gcatggctgg aataacttgc gcttgataac caatcatctg gacttgagca aaaccaaaat
    16921 cccaaaatgg aatatctgct gcggctgtgg aagctacact ataagcattt aataattgcg
    16981 gagaaactaa cgtaattcct aaaacgattc ctaatatttg tgtagtaccc atcttttttg
    17041 caatactcca agtgatacct actggaagaa aatggaaaat tgcttcaccg ggtaaccaca
    17101 aaaaggcatt gacaccattc caaaacttcg agtgttcaac gattgttcca ccaagacttt
    17161 caaactggat accttcaagt acatttctga atccaaggat caatccaccg acaatgattg
    17221 caggaatcag tggtgtaaag atttctgcta aaacggcaat agcacgttga acgggatgaa
    17281 ggttttgttt tgcagctgct tttccttgtt ctttggaaac cccttcaact ccagatactg
    17341 caacgaagtc gttataaaaa gtagaaacat cgtttcctat gatgacttgg aactgtcctg
    17401 cattcgtgaa catccctttt acgctaggta tatcttcgat cgctttttcg tccgcttttc
    17461 ctggatcatt caggacaaaa cgcatacgag tcgcacaatg actaactgca gcaatatttt
    17521 cttttccgcc gatatcatgt aacagcttct cggcatctgc ttgatatttt gccatatcaa
    17581 taaacccctt tctcttaact tgtctatgca agttgtttct tcatttacta tacttttttt
    17641 cggagagaat tgcaagcgtt ttattaaaaa gtacgagaga aaatgatatc gttttaaaaa
    17701 gcttaaaata agcttttctt aaaaatagga acattgaaat acaaaaagag ctgtagtata
    17761 gtgaagaaat gatggaagaa cgagtgaggg aaaaaacatg aataagttca atgaaatttt
    17821 tttagatttg gaacagaaaa ttttagcgaa agaatatcct ccgcattcgc ttttacccag
    17881 tgagaaccaa ttgatcaaaa tttatggtgt ttcacgagaa acaattagaa aagcgctgaa
    17941 tcttttgact acggaaggtt atattcaaaa aaaacaagga aaaggttcga tcgtactgga
    18001 tcggaatcgc ttcgactttc ctatttctgg attgactagc tataaagagc tacaggagac
    18061 ccaacgtatt ccaagtgaaa cgatcatcca tacgttagaa gaaacagaag tgacaaaagc
    18121 attgaacgag atcactggct gggagatcgg tgcgcctgtt tggcatttga tacgcgaaag
    18181 aaaaatcaat ggcgaagtag ttatattaga caccgattat ctattaaagg aaatcgttcc
    18241 tattttaact ccagcacaag cacaaggatc gatctatgaa tattttgaaa atgagctttc
    18301 tttgacgatt gcttacgcgc aaaaggagat cacagtagaa gaagtaacgg aaatcatgaa
    18361 aacagcgatg gatcttcacg atgacactta tgcagtagta gtcagaagcc tcgttcatct
    18421 ggaagatacc cgctgtttcg agtattctga gtcggttcac cgattagata aattccgttt
    18481 cgttgagttt gcccgacgcc gaaaagtgta aatagttcaa aagaaagcaa attgttggca
    18541 tctgtccact ttggcgctat gataggatca caaagggggc atttttaatg gataaaccaa
    18601 taagaccaga aaaagaagaa ctgaaagaaa agttatcacc catggcttat gcagtcacac
    18661 aagaaaatgc aacagaacga ccatttagcg ggaaatatga tgatttttac gaaaagggga
    18721 tctatgtaga tatcgtcagc ggtgaaccgt tattcagctc tgcagaaaaa tatgatgcag
    18781 gttgtggctg gccatcattc agcaaaccaa taacgaaaag acaagtgcgc gaaaaagccg
    18841 atttttctca tggcatgcat cgaatagaag ttcgaagcaa agaagcagat tctcatctcg
    18901 gacatgtgtt taccgatgga ccagtagatc aaggtggtct acgttactgc atcaattctg
    18961 cctcactgaa attcattcca tatgaaaaga tggatgaatt aggttatggg gaattcaaat
    19021 cacttgtaga ataatggaaa tacacaaaga gcggtacaat acggggattt actcgatcgt
    19081 ccgctctttt tttattcaag cgattgctat ttgaaagaaa cgctccctag actccttttt
    19141 tcacaaggtg aaatgtgcta caataaaggg aacgtatgtg tgcaaaagaa agaggactag
    19201 aatatgtatg aatatctgac aggtgtggtt acctttatca acccctatta catagtagta
    19261 gaaaccaatg gtatcggcta ccagatcgca ctaggaaatc cttaccgcta cagtagcaaa
    19321 ctaaatcaaa aaataaaaat ttatgtccat caggtaatac gtgaagatgc gcatcttctg
    19381 tatggatttg attcgctaga agaaaagcaa ctgtttttgc gattagtaag tgtttcagga
    19441 atcggtccga aaagtgccct tgcaatcatg gcaagcgaag accactccgg tttgattcaa
    19501 gcagtagaat ccagtgatgt cacttatttg acgaaatttc caggagtagg gaaaaagaca
    19561 gcacaacaaa tgatcttgga tctaaaagga aaattcggtg agctgagtat cgatacacct
    19621 tttagtctat ttgatgaagc aaacacaaaa gacgctacag ctctatctga agcaatggaa
    19681 gctctctcgg ctcttggtta cagtgatcga gaaatcaaac gtgtagaaaa acaattaaaa
    19741 gaagtagata atcagacaac ggatgagtat ctacgccaag cactcaagct aatgatgaaa
    19801 aaataaggag gagaccaaat gacagatgaa tacttgttat caccagaatc tggcgaaaat
    19861 gaactgagtt tggaaaaatc tcttcgacca cagctgcttt ctcaatatat cggacagaga
    19921 aaagtcaaac aagaacttca gatttacatc gaagctgcca gaaatcggga agaaccttta
    19981 gatcatgttc tgttgtacgg gcctcctgga ttaggaaaaa cgacgatggc catggtcatt
    20041 gctaatgaga tgaccgtaaa tattcgtacg acgagtggcc ctgctatcga acgtccgggt
    20101 gacttagtag caatcttgaa tgaactggaa gcaggggatg tcctgtttat cgatgagatc
    20161 catcgattac ctagagtggt agaagagatg ctttattcag ctatggaaga tttctatgta
    20221 gatatcatgg taggacaagg gccaaccgca catccggttc atttccctct tcctcccttt
    20281 actttgattg gcgcgacgac acgggcaggt atgctttctg cacctttacg agatcgattt
    20341 ggcatcattt ctcatatgga atattatgac gaacaggatc tgcaggagat cgttttacgt
    20401 tcttcggata tttttcaaac agagatcatt gaagaagggg cattggaaat tgctcgccga
    20461 tcacgaggaa cgcctagaat cgctaatcgc ttgctcaaac gggttcgtga ttttgcgcaa
    20521 gtccaaggag acgggaaaat cgatcgtcct atagcggatc aggcacttac tttgctacag
    20581 gtcgatcagg ctggattgga ttatgttgac cagaaattgt tgcgaacaat gatcgagtta
    20641 tatggcggcg gaccagtagg gttaagtacg ctttcagtaa acataggaga agagcgtgag
    20701 acggtagaag atatgtatga accgtatctg atccaaaaag gatttttaaa gcgtacacct
    20761 agagggcgaa ttgctactgc ttatgcctat gaacattttg gttacgacta tcatatatga
    20821 tttttgcttt tagattggag gaaaatgaat gagtgataca acacgtacga aacaattgat
    20881 caaaaacgta ttgattgatt tgtgccaaac gcaatctttt cgaaaaatca gcgtccaaaa
    20941 aatcgcaaac gaagcaaaaa tcaatcgaca aacattttat tatcatttta cagataaata
    21001 cgatttgctt cgttgggtct attgtgagga tgcattgcat tatttagatc cacaatgtct
    21061 ttcacttgat aattgggaag agcaagcatt gaaaatgctg aaagcaattg ctgagaagaa
    21121 agaattttat gcaaatactg tgctttctga cccagaaatt ttacaaaaag agttttctca
    21181 actgatccaa aaatcattta tcaagatttt tgaacaaatg gatgaagaaa gacaactttc
    21241 tgcaagagac aaactatttt atgctcgttt tttttcttac ggttgcagtg gtgtattagt
    21301 caattggatc acgaatgaat atgaagaaac accgatggaa atcgctatgc agcttttccg
    21361 tttagccaag gatacggaat tatattctta tcgtctttat gcaaaagaag agttggaaga
    21421 acagtaatag aaaaattttt tgttggaaaa gcaataatcg ctaaaaggag atggggaaat
    21481 gacaaaaatt ttatttgttt gtttgggaaa tatttgccgt tcaccgatgg cagaaggctt
    21541 gctgagaaaa cgaatagcat tagaaggaag ggaagcagaa ttttttgtgg attctgctgc
    21601 tacaagcacg tatgaagttg gaaagacacc gcaccctgga acgaaaaaaa tattaaacca
    21661 agaaaaagta gatatgacga atatggttgc tcggcaaatc acaccccacg attttgaaac
    21721 ttttgattgg atcattggca tggatcaaga aaatgtagaa gaactgaaac gaagagcacc
    21781 acgctcagcg caggggaaaa tccatttgtt tttaagtagc attcctggaa aagaaaaaga
    21841 aaatgttccc gacccatact ataccaataa ctttgatgag acatatcact tgatcacaga
    21901 aggcctgaat cattggtgga atatttggat tgatgaaaat aaaataacaa acaatttgta
    21961 aagttaacgc tttcttttta aggcgatgtt agagcatttc cttgtttttt tgtaaaatgt
    22021 taggtaaaat aaaattaatt aagtaacact ttcttagcta aaggaggagt gcattcgatg
    22081 aacgaagaga cagtatcgcc aatgaagaag cttacgaagt tagttactca tacttcgtat
    22141 caactttttg attcttttgt aaatgacact gaagaacatg ctaagaatgt acgattgcaa
    22201 ggaaagttta ttcttgagct gcaaaaagct gctactgaac atagtctagt tatcttacaa
    22261 gtaagagcaa atcgtttgag tagcaagtat gaaacgatct caggctgggt ggcgacaaaa
    22321 acggtcgaaa aagaccgagt gatgatccgc ctacaaaacg aagagcagca gatccggatg
    22381 gttcctgtag aacaaattga aaaaataaca atcctttcac cagcagggga acaggaacgg
    22441 ttatcaaaat aatttatacc agatagaaaa gcacgtatga gcttgtacca tcaatcatta
    22501 ctaatcaaaa ataaccgaat gctttaagct gatatcagta aaacggaacg ttttaggatg
    22561 agtatcatag catgacagca ttcggttgat tagttgaatc gattggatgg tacaagtttt
    22621 tttgataaaa aaaggtcctg ctagccgggg agctagcagg ataaggagtt aaaaatgaaa
    22681 aagtgttgtt agggttgttt gttggtatgc ttatataata gtcattagtt gtgaatattt
    22741 tgtgacgaaa gttttcgaaa gttgttaag
//
LOCUS       DASJST010000036        21939 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJST010000036 DASJST010000000
VERSION     DASJST010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.036"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="U4B05_001112"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="U4B05_001112"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN2050264.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="U4B05_001113"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="U4B05_001113"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN2050265.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="U4B05_001114"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="U4B05_001114"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN2050266.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="U4B05_001115"
     CDS             complement(3374..4681)
                     /locus_tag="U4B05_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN2050267.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="U4B05_001116"
     CDS             complement(4684..5265)
                     /locus_tag="U4B05_001116"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN2050268.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="U4B05_001117"
     CDS             complement(5837..7129)
                     /locus_tag="U4B05_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2050269.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="U4B05_001118"
     CDS             complement(7126..7749)
                     /locus_tag="U4B05_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050270.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="U4B05_001119"
     CDS             complement(7752..8588)
                     /locus_tag="U4B05_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2050271.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="U4B05_001120"
     CDS             complement(8605..9492)
                     /locus_tag="U4B05_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2050272.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="U4B05_001121"
     CDS             complement(9508..10791)
                     /locus_tag="U4B05_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2050273.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="U4B05_001122"
     CDS             complement(11474..12157)
                     /locus_tag="U4B05_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN2050274.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="U4B05_001123"
     CDS             complement(12175..12792)
                     /locus_tag="U4B05_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2050275.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="U4B05_001124"
     CDS             complement(12802..13626)
                     /locus_tag="U4B05_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN2050276.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="U4B05_001125"
     CDS             complement(13619..14179)
                     /locus_tag="U4B05_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050277.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="U4B05_001126"
     CDS             14353..15309
                     /locus_tag="U4B05_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2050278.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="U4B05_001127"
     CDS             complement(15614..16570)
                     /locus_tag="U4B05_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN2050279.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="U4B05_001128"
     CDS             16674..17276
                     /locus_tag="U4B05_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2050280.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="U4B05_001129"
     CDS             complement(17364..18863)
                     /locus_tag="U4B05_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN2050281.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="U4B05_001130"
     CDS             complement(18879..19403)
                     /locus_tag="U4B05_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN2050282.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="U4B05_001131"
     CDS             19701..20450
                     /locus_tag="U4B05_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2050283.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="U4B05_001132"
     CDS             20441..21226
                     /locus_tag="U4B05_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN2050284.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="U4B05_001133"
     CDS             21195..21911
                     /locus_tag="U4B05_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2050285.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
BASE COUNT         6461 a         4737 c         3838 g         6903 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagt
//
LOCUS       DASJST010000037        20801 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJST010000037 DASJST010000000
VERSION     DASJST010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.037"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..300
                     /locus_tag="U4B05_001134"
     CDS             <1..300
                     /locus_tag="U4B05_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2050286.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="U4B05_001135"
     CDS             complement(724..894)
                     /locus_tag="U4B05_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2050287.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="U4B05_001136"
     CDS             complement(1088..1267)
                     /locus_tag="U4B05_001136"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050288.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1277..1501)
                     /locus_tag="U4B05_001137"
     CDS             complement(1277..1501)
                     /locus_tag="U4B05_001137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050289.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1654..2766
                     /locus_tag="U4B05_001138"
     CDS             1654..2766
                     /locus_tag="U4B05_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2050290.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="U4B05_001139"
     CDS             complement(2844..3137)
                     /locus_tag="U4B05_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN2050291.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="U4B05_001140"
     CDS             3305..3619
                     /locus_tag="U4B05_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN2050292.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="U4B05_001141"
     CDS             3640..5619
                     /locus_tag="U4B05_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2050293.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="U4B05_001142"
     CDS             5632..5958
                     /locus_tag="U4B05_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN2050294.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="U4B05_001143"
     CDS             5974..6492
                     /locus_tag="U4B05_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050295.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="U4B05_001144"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="U4B05_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2050296.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="U4B05_001145"
     CDS             complement(7928..9433)
                     /locus_tag="U4B05_001145"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN2050297.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="U4B05_001146"
     CDS             complement(9525..10115)
                     /locus_tag="U4B05_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2050298.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="U4B05_001147"
     CDS             10294..11940
                     /locus_tag="U4B05_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2050299.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="U4B05_001148"
     CDS             11989..12708
                     /locus_tag="U4B05_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2050300.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="U4B05_001149"
     CDS             13187..14134
                     /locus_tag="U4B05_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2050301.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="U4B05_001150"
     CDS             14146..15066
                     /locus_tag="U4B05_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2050302.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="U4B05_001151"
     CDS             15107..16564
                     /locus_tag="U4B05_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2050303.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="U4B05_001152"
     CDS             16909..17523
                     /locus_tag="U4B05_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2050304.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="U4B05_001153"
     CDS             17516..19240
                     /locus_tag="U4B05_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2050305.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="U4B05_001154"
     CDS             19237..20688
                     /locus_tag="U4B05_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2050306.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3510 c         4166 g         6416 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DASJST010000038        20493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJST010000038 DASJST010000000
VERSION     DASJST010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.038"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..562)
                     /locus_tag="U4B05_001155"
     CDS             complement(<1..562)
                     /locus_tag="U4B05_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN2050307.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(749..1399)
                     /locus_tag="U4B05_001156"
     CDS             complement(749..1399)
                     /locus_tag="U4B05_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050308.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="U4B05_001157"
     CDS             complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="U4B05_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN2050309.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2911..3909)
                     /locus_tag="U4B05_001158"
     CDS             complement(2911..3909)
                     /locus_tag="U4B05_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN2050310.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="U4B05_001159"
     CDS             complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="U4B05_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN2050311.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4925..5656)
                     /locus_tag="U4B05_001160"
     CDS             complement(4925..5656)
                     /locus_tag="U4B05_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN2050312.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5660..6925)
                     /locus_tag="U4B05_001161"
     CDS             complement(5660..6925)
                     /locus_tag="U4B05_001161"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN2050313.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7188..10004)
                     /gene="uvrA"
                     /locus_tag="U4B05_001162"
     CDS             complement(7188..10004)
                     /gene="uvrA"
                     /locus_tag="U4B05_001162"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN2050314.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10014..12008)
                     /gene="uvrB"
                     /locus_tag="U4B05_001163"
     CDS             complement(10014..12008)
                     /gene="uvrB"
                     /locus_tag="U4B05_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN2050315.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12267..13769
                     /locus_tag="U4B05_001164"
     CDS             12267..13769
                     /locus_tag="U4B05_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN2050316.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13771..14508
                     /locus_tag="U4B05_001165"
     CDS             13771..14508
                     /locus_tag="U4B05_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2050317.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14553..14726)
                     /locus_tag="U4B05_001166"
     CDS             complement(14553..14726)
                     /locus_tag="U4B05_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050318.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14702..15367
                     /locus_tag="U4B05_001167"
     CDS             14702..15367
                     /locus_tag="U4B05_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050319.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15449..16615)
                     /gene="dnaJ"
                     /locus_tag="U4B05_001168"
     CDS             complement(15449..16615)
                     /gene="dnaJ"
                     /locus_tag="U4B05_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN2050320.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16766..18595)
                     /gene="dnaK"
                     /locus_tag="U4B05_001169"
     CDS             complement(16766..18595)
                     /gene="dnaK"
                     /locus_tag="U4B05_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN2050321.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18646..19209)
                     /gene="grpE"
                     /locus_tag="U4B05_001170"
     CDS             complement(18646..19209)
                     /gene="grpE"
                     /locus_tag="U4B05_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN2050322.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19303..20346)
                     /gene="hrcA"
                     /locus_tag="U4B05_001171"
     CDS             complement(19303..20346)
                     /gene="hrcA"
                     /locus_tag="U4B05_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN2050323.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
BASE COUNT         5820 a         4460 c         3503 g         6710 t
ORIGIN      
        1 ctataaacat caccccaaat aaaacagaaa acagtataag cattataaaa attatcaaca
       61 accttgatcc ttcttttata gattcaggaa acagtataat tttagtacta gataaattgt
      121 ttacaccgat tagtgttaaa atatctttat tcttcttgct aacataaccc tttatgcaca
      181 aaagaacagc tatacttaaa gtaacaaata aataacgtat tccaatagaa aaatcactat
      241 aaatactctc tagcatagga ccgacaattg aagaagtcac tactgcaaat gctggaccta
      301 tccaagcaac attagaatgt tctttctctc ttttcctttg taaatctttg ttgagtatta
      361 atctatttat ttcttctgca ggcaagttaa ttttcataac cttatggggg attatccaat
      421 taaaaatcgg aaaaaggtag cctccccaaa aagtatagtg atctgtgtca atcagataat
      481 aacctgattt tgattccaga ataacatatc tcaaagccga acgaaaattt ttcatgaata
      541 tccataatct cactgtttcc acacctaatt cctccaattc caattaaatg gatttatagc
      601 atcaccaatt atgttaactg cctgaccaac ctcattgata aaattggcac cactatccca
      661 tgcttcaaca atgctatata tctatcaaac aaaaaatgga aaactaaata ctcgcgcaca
      721 taaattttac attctgtttt ttataagact aataataatc attgaaaaaa tttcttatcg
      781 gtggcgcata ttcccaattg attaatgtga tcccatattc tgcaagtatt ttacgagctt
      841 cttctaagtc atagtgagaa aaatgcataa aacctgcaat acgatcacgg actgtgagct
      901 gtggcttgct tccaccatca ttcataaaat atccagcaga gccactactt aaagtgattg
      961 ttacatcttc actttctatt attaagaatt cataatgaaa tgctttcaac tctccactat
     1021 tatttaaaag ttttatactc caacttcgtc taggttgagt tgtcacattc cctctaaaac
     1081 ttcctataaa ttctgcaatc accgtatctt caatcaaaat tctttcctca tctatttcat
     1141 attcaagact aaacggaaat tctccatatg taatctctgg caatggtggt gctgtagttc
     1201 tccaaataaa tacaataact gtgaatagaa aaaatccaac gatcgatata aatgttatga
     1261 gacctgctcg aagtaaaacc cgatataata aaaatgctgt atttcgcttt gctatatcta
     1321 gaaaaataaa aagtactgca atcataatga tagtaaaagt tgtaaaaaag cacatttttt
     1381 gccctccctt tataaacatc gattctcaaa ttctaaacca aatcttgata gttcaagttc
     1441 aggtataacg tataccaacc atacatccca attactattt atgaacttat gattatcact
     1501 gaataaatca ctccatcccc ttgttaaaac ttctacatta attacacctg gttgttttcg
     1561 actagctaat ccaatgaact tatcaaccat ccttatccac caaccacgag aatcttgacc
     1621 agaagtaaga aaaacagttc tcaaggttga tcgagcagtt ccttccagtt tttaattaat
     1681 ctccagttat agataaatac ttgatcctat ctatatcagg acttctatat taagcaaaag
     1741 tttatgtata aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt
     1801 aaaaaacaat agaagccttt caacattatg ttactccagt aaagttggaa ttatagtttg
     1861 acggaagaaa agcaagtata gcttttaaaa aaaactgata cattcgcatc tcctgcttca
     1921 tgtatcagtt ttttctttgg attaaactgc ttcttcacgc agtttttccg caaattcatt
     1981 tattttgcga attcggtgat taattcccga ttttgatatt gcacctgagg gaatcatttc
     2041 ccctaattct ttcaagctga cctctggatg ttgaagccga agttctgcga tttcctgaag
     2101 tttttcagga agggcatgta gaccgacctt tgcttcgatc aattcgatgt tttcgatttg
     2161 tttagaagcc gcatcaatcg tcttgttcag atttgctgtc tcacaattga ctagacgatt
     2221 gaccgaattt ctcatatccc gtacgattcg aacatcttca aatcggagca ttgagttggt
     2281 tgcaccaatc aatgttaaga aatcagcaat tttttctgct ccttttaaat acgaaatata
     2341 gccattgcgc cgttcaagcg ttcgagcatg tagaccgtaa taatttaaca tatcgcaaat
     2401 gtcttggtta tgctgttcat aaatagaaaa tatttccaaa tgataacgac tcgtttccgg
     2461 attattgact gaacctgagg ccataaaagc accacgtaag taagaacgca ttttttgcga
     2521 atttcccata atctcgtccg aaacgtgtcc attgaacatc ataccatcca tgatatcaag
     2581 atccatcaac acatcttttg tgccttgctt caaccggacg atatagacat tgttcttttt
     2641 taatttcatt tttttacgaa cgagtagctc acttcgtacc tgataatgat ctttcaaaag
     2701 agaatagata cgtctggcaa ttgcagcatt ttctgtttgg acgttcaaaa taaactgctg
     2761 gttcacaata cctaaagagc cgttcattcg cagcaaagca gctaattccg ctttcgcatg
     2821 ttctcgatga acttccaaac cagttagttc tttcttcaca tctgcagcaa atgacatcct
     2881 attctcctcc tttctccaac aataaattgc ttaatatttt gaaccgaaaa ctaaacggaa
     2941 caattcttct actactttgt ctccatcgtg gaagacacca ccatctttca attctaaaaa
     3001 gtcggtggaa atcactcgac atccttcatt tctcaatccg ttgaagtcgt gtgcgacttg
     3061 tactagatac tcatcataga tctctgggtc catataatct tcaggcactt tttccgtatt
     3121 gaccaatact gtatcgataa aaggctgatt caaatgcttg tgtaaaacac gtatatgatc
     3181 ggcatctgtg aaatgttccg tttccccttt ttgggtcatg atattacaga tatagacggt
     3241 ttcagcagtt gtcttgagca ttgcttcgcc gatttctggt atcactaaat ttggaaggat
     3301 actggtgaat aaacttccgg gtcccaaaac gaccatgtcg gcgtccataa ttgctgaaac
     3361 gacttttcga gccgctttgg cttcactttt atcatttgtg ttgcagacat acacacggtc
     3421 aatggtttta cggttgatcg cgattttaga ttctcctact gcagtcgttc cgtctttaaa
     3481 aacagcatgc aaggtcaaag gagtttctga agaaggataa acatgtccat ctacatgcat
     3541 catttttgct aataattgaa tcgcctcata agtgcttccc cgcatctcgg aaactgcggc
     3601 aatgatcaga ttaccgatcg tatgattggc taaaaactga tcttcctttt tgaaacggta
     3661 ttggaaaata tcttcataga attgtggcat atccgataac gccactaaga catttctcaa
     3721 gtcaccagga ggagccatag cgatcgattc tctgattgca ccgctgcttc caccatcatc
     3781 tgcaacagtc actactgctg tgatatctgc tccttgattt cgtaaacttt tcaggataac
     3841 tggcagacca gtgccaccgc cgaccactac gatctttggt tttcttatcc ggtaggtttt
     3901 cattttcacg accggttcac cgtctctttc cttttatcct tgtcccgatg tgtcacattg
     3961 actttatagt ttttccctaa agcatttgcc aaacgttcag tcaatgccac agaacggtgt
     4021 tgaccgcctg tacagccgat tgcaactgtc aagctgcttt ttccttcttt aatgtatcca
     4081 ggcaatacag tttccagaag atccaaaaat ttatggtaga atgattgcgt ttcatcaaag
     4141 gacatcacat aatcatagac tggctgatct tttccggtca atggtcttag ttccgggata
     4201 taatgagggt tgggcaaaaa acgcacatcc atcacaatat ccgaatcgat cggcaacccg
     4261 tatttaaaac caaatgatac aaattcgatc cgaaatcctt ggtctccctt tgatttgaat
     4321 gactcattga ttttttcacg taattgtcgt ggtgtcaatg ttgtcgtatc aatgatcacg
     4381 gaagcttggg ctttcaagtc gtctagtatc gcccgttctt tacgtatccc ttcagtgatc
     4441 aaaccgtcca tcgccattgg atgtgctcgt cttgtttctt tgtagcgagc cactagttct
     4501 tcgtctgaag catctaaaaa taagatagaa gtgtcgatga agttcgtatt ttcgatttcg
     4561 atcagcatgt tttggatttc ttcaaagaag gaacgagaac gcaaatccac tcctaaagcc
     4621 atttttgtga ctttacctga ttctttgatc aattcccaga attttggaat caatcgaggc
     4681 ggcatattat cgatacaaaa atagcccata tcttcaaaac tttggatagc tactgttttc
     4741 cctgcaccac tcatacctgt tatgatgact aattgcaaat tatctgtcat atttacgaac
     4801 ccctctcaca ttgtgcataa taaaattata tcatcccggg cgtatcatta ccagttttct
     4861 ttttctttta caaaaagatt tggaccaaat atcttttagg atatttgccc caaatccaat
     4921 ttttttactt ttccgatgct gtatccaatg cctcgcttct ttcagctaat gcacgttcga
     4981 tcgttcgatc cgctaaaata gattgagcat cgatcactgg gtaatgatta tcttcagcaa
     5041 attcatgcat caaagataat tctgtgcaac ctaggatgac tttttcacag tttaggcgct
     5101 ctaccgcttc ctctaagatt tcgtaataga gctcttgatt caaataatcc gattccttga
     5161 tctcatgata gatcaggtaa ttgatttttt cttgaagcgc agtatcagga atcactgttt
     5221 cgaaaccaag atttttgact tcgcgttcat agattccagc tttcatactt ccttctgttc
     5281 ctagaatcgc tactctccct gtcgtatgct gacggacaag ttcatttgct gcttctcttg
     5341 gcatatgcaa gataggaata tccgttgccg cttgtaactc ttcaaaaaag tagtgtgcgg
     5401 tgttacaagt caacacaatg aagtttggtc gcaataaatt ttgtttctca atatcatcca
     5461 gcagaaacgg cattggattt tcttctgatc gatctaaaat ataagctgta cgatcaggaa
     5521 ctgttgcatg attgaataag acataattca aatattcttg atcttttgta gctttcgttc
     5581 ggtggttgat caagcgaaca aaactttctg tcgccatcgt gcccattccg cctaaaatac
     5641 tgaaaaaatt ctccatgaac tagccttctt tcacatgaaa atactttttg aatcttggaa
     5701 tatagttatg gaacatatat ttcatcaaca accagcgctt gattgatcgg tctttttcat
     5761 aaaatacagt agtaccgtaa cgattttctt tgatcatttg ctcagctagt tttttgtctt
     5821 cattttcccg agcatattct aaaaagattt ttttgggcac acccatccat aatcttgctt
     5881 tatctgattg attcgttcca taggtcgttt caacaaaggg tttgttgaac actcgatctt
     5941 ctgtgacaaa acgagccaga ttcaagccgt tcaatgtcac aaagaaactg ctacgacctt
     6001 gtctcaaatt gatttcaaat aatttatatt ctccatctct aggatcatac ttcatatcga
     6061 aattggcaaa gcctgtatat tcgatttttt ccaaaaatgc cttaatggtt tgatagattt
     6121 tttcgttata atctggcatg atcacaacgt aattaccaat cgatgctggt gtaggatctt
     6181 ctaatagtgg atgaccaagg cacatcatcc gaacttgatg atcttcatct acatacgcat
     6241 ttagcacacg catattacta tcatcacccg ggataaaatc ttgtacaatc atttcacttt
     6301 tataaccggc ttcgtatatc cggcctaaaa ttaaatcgaa ctcttctcta gtctctaaaa
     6361 taaaagcttt ttttcttcct tcaaattgga cagaaagata ctccacactg tttgcaggtt
     6421 tcaaagcaac tgggaattca aaaggcaatt cctgttccaa atgtccattt actaacattt
     6481 cttctctgac gatcaatgtt ttaggatacg gaagatcata ttcttcacaa acttcataaa
     6541 agcttacttt attgatcagc ctttcaaata aagaataatc aatatatgga cagataaaat
     6601 attctgacag ctcttgttta tgttgggaga tcaattctgc atatccatca ccacaagcaa
     6661 tcaaaagata ttttttactc ttatcagaat atctttcttt tgccaaacga agcatcgtct
     6721 cgataaatac agggtcttta tcaaatcctg gaataacttc tacattgaca attttactgt
     6781 agcgggtagg ggctaactga tccgaagcat atgcttggca gattttgccg tatgcttcat
     6841 taaaagaacg tgccatacca taaacgttca tgtcgcttcc taataatata gggataaatt
     6901 cttcattttc aatactgttc atcattatct ctcacttact cataatctct tgtttttctt
     6961 ttccaactac acaaggaata tcataccata aagaaaaaag caatcaaagg aaagtcctcc
     7021 gattgctcaa ttcttttttg aacggattga acttatagga agtttttatc tataccaatc
     7081 agtgtagaaa gaactaccat gtaatgattc tagaacactg atattaataa aacaagcaaa
     7141 aattcatttt tttatgtttt tttaactatc tcagctatct atcctcttta taccattatt
     7201 tttttcaaat agtgacctgt ataactttct tttacgttaa tgatttcttc tggtgttcca
     7261 gtcgcaagga tcgttcctcc accttctcca ccttctggtc ctaaatcaat gatataatct
     7321 gctgttttga ttacatctag attgtgttca atcactaaaa ctgtgttacc agcatctact
     7381 aatctttgta atacatgcaa caagcgggcg atgtcatcgc tatgaagtcc tgtcgttggt
     7441 tcatctagta tatagaaatt ctttccatta gagattttgt gcaattcact ggcaagtttc
     7501 atccgttgtg cctcaccacc ggacaatgtc gttgctggtt gccccatcgt cacataacct
     7561 aagccaacat caacaatcgt ttgcagtttg cgatgaatct ttggaatgtg cttgaagaat
     7621 tctacagcat cctctactgt catttccaaa atatcagcaa tgctttttcc tttgtaatgc
     7681 acttctaatg tttcagaatt atatcgttta ccatgacata cttcacaagg aacatagaca
     7741 tcaggcaaaa agtgcatttc gatcttaata attccatccc cgcgacaagc ttcacaacga
     7801 ccgcctttta cgttgaaact aaaacgccct ttcttataac cccgcatttt tgcctcgttc
     7861 gtttgagcaa ataacccacg gatatcatca aatacacttg tataagtcgc tggattactt
     7921 ctcggcgtcc ggccgattgg gctttgatcg atatcgatga tcttttcgat actttcgtag
     7981 ccggaaattg tcttgaattt acctggctta gcagaattct tatttaattt ttgcgctaac
     8041 gatttcttta agatactatt gaccaatgta ctctttccag aaccagacac acctgtgact
     8101 gctacaaatt ctcctaatgg aaattcaaca ctaatatttt tcagattgtt ttctgaagca
     8161 ccagtgatct tgattgcttt cccattacct ttacggcgtt cttttggtac agggattgat
     8221 tttttccctg aaagatattt ccctgtcaaa gaattttcat cattagctac ttcttcagga
     8281 gtgcctgcag ccacgatttg tccgccttgt tctccggccc caggtccgac gtcaatcaaa
     8341 taatctgctg cccacatcgt atcttcatca tgttcgacta caacgagggt gttgccaaga
     8401 tcacgcattt tctttaatga gccaatcagt cgatcgttgt cgcgttgatg caatccgatc
     8461 gatggttcat ctaaaatata caaaactcca gaaagattcg atccaatttg tgtagccaat
     8521 ctgattcgct gcgcttctcc cccagataat gttccggaag cgcgactcaa agttagatag
     8581 tctagtccca cattttccaa gaatgaaagt ctgtcattta cttcttttaa aatcggtttg
     8641 gcaattactt tctcttgttc agataacgtt aagtttgaga agaaatctac tgctttttga
     8701 atagacaact catttgtttg cccgatattc gttccattga tttgaacaga tagagcttgg
     8761 ggattcaatc gataaccttt acatgtctga caagttaatt ctgtcatata aagacgcatt
     8821 tgctcacgcg tgaaatcact gttcgtatca tgataacgac gctcgatatt cttcaatacc
     8881 ccttcgaaag tcgtttctac atcgcgaacg ccaccaaaat cattttcata atggaaatgg
     8941 aaaagatcat ctgtcccatt caagatgatc tcctgatgct cttccggtaa ttcttcgaat
     9001 ggtgtatcta aatcgatacc gaaactttca caagcctgcg ctaacatttg aggataatac
     9061 tgagagctga ttggattcca tggaacgata gcaccttgac taagtgtctt cgttctatct
     9121 ggaatcacta aatcttgatc gacttctaat ttgacaccta aaccatcaca gtctggacaa
     9181 gctccaaaag gcgcattgaa tgagaacagt cttggttcta gttcgccaac agtaaatccg
     9241 caataaggac aggcatagtg ttcgctaaac aacatttctt cttcaccgat cacgtcaacc
     9301 agagcatagc cgtctgctaa tcgcagggcc gcctctaaag aatcaaatag gcgagaacga
     9361 atcccttctt tcacaatgat ccggtcgatg acgatcgcga tatcatgttt tttgtttttc
     9421 tccagctcag gcacttcact taaatcatag atctcaccat cgactcgaac gcggacgtat
     9481 ccctcttttt ggattcgttc gaatattttt ttatgttgtc cttttttctt taccacgatc
     9541 ggtgccaaca cttggatctt tgtgcgctca ggcaaagcca ttacttgatc tgccatttga
     9601 tctactgatt gactggtaat ctcgatatga tcgttgggac aaatcggatg tcctacacgt
     9661 gcaaacaaca agcgtaaata gtcattgatt tccgtcacag tacctactgt cgaccgagga
     9721 tttttacttg ttgttttctg atcaatagaa atcgccggac tcaatccgtc gatgctatct
     9781 acgtccggct tatccatttg tcctaaaaac tgacgagcat aagcagaaag gctctctacg
     9841 tatctccgct gcccttcagc gtacagcgta tcaaatgcta gggaactttt tccagaaccg
     9901 gacaaacctg taacgaccac gaatttatct cgtggaatcg ttacatctat atttttcaag
     9961 ttatgggcac gtgcaccatg aatcgtaatt ttatcgttag ccatgttgtc ctcctacttt
    10021 gaagctttta attctaaaat catatctcgc acattcgcgg ctgtttcaaa atccaaagct
    10081 tttgccgcat ctttcatttc tttttctaat ttcatcaata acgtgtcttt ttcttccttc
    10141 gtcatttctt cgtacgagac ttgtaccgct tcttttgtat catcttccga ttctttcgta
    10201 atagaaatca aatctcggat ttctttaatg atcgtttttg gaacgatccc atgttcttca
    10261 ttgtacttct gctgaatcgt acgacgtctt gacgtttcat ccatcgctag acgcatagaa
    10321 tctgttactt tgtctgcgta catgattact ttaccttctt cattccgagc agctcgtcca
    10381 atcgtttgga ctaaggaacg ttcactacgt aaaaagcctt ctttatccgc atccaagata
    10441 gcaacaaggg aaacttcagg tacgtctaat ccttcacgaa gcaaattgat tccaactaac
    10501 acatcaaatt cacctaatcg aagatctcga atgatttctg ttcgttccag cgttttgata
    10561 tcactatgca aatatttgac tttgatccct aattctttga aataatctgt gaggtcttct
    10621 gccatttttt tcgtcaatgt cgttacaaac acccgctggt ctttttcgac tcgttcattt
    10681 atttcgccta ctagatcatc gatttgtccc atgattggac gaacttcgat cactgggtca
    10741 agcagccctg tcggacggat gatttgttgg ataacggtat ctgtttgttc atgttcatat
    10801 ggtcctggcg tcgcagagac ataaatgatc tgattgacat gtttttcaaa ctcttctaac
    10861 cgaagtggcc gattgtctaa agcagatggc aaacggaaac cgtaatctac tagcatctgt
    10921 tttctcgctc ggtcaccatt atacattcct ctgatttgag gcatggttac gtgcgattcg
    10981 tccgccacga tcaaaaaatc ttcaggaaag aaatccaata gcgtataagg cggttcacct
    11041 tctttacgtc cgtccatatg ccgagaataa ttttcaattc ccgacgtgta gcccatttct
    11101 aacatcattt caatatcgta attggttcgc tgttctaaac gttgagcttc cagaagtttg
    11161 ttttcatttc gcaagacagt taaacgctgt tctaattccg ctttgatatt cgctacggca
    11221 tgctccatat gttcatcatt cgtcacaaag tgagtcgccg ggaagattgc tacgtgttcc
    11281 gtttctccta aaacttcacc tgtcagtgca ttgacttctc ggatacgttc gacctcatct
    11341 ccaaaaaact ccacacgtaa ggcacgttca tctcttgaag ccggaaaaat ctccacgaca
    11401 tcaccgcgca ctcgaaaacg accgcgctga aaatctatat catttctttc gaattggata
    11461 ctgactaagt cacgaatcaa ctgattgcga tctagttctg ctccctgtcg aatcgaaact
    11521 acttgttttt ggtactcaaa aggcgagcct aatccaaaaa tacatgaaac ggatgcaatg
    11581 acgatcacat catttctttc taataatgaa ctagtagccg aatgtcgtaa tttgtcgatc
    11641 tcatcattta cacttgaatc tttttcgata tacgtatcgc ttgaaggcac ataagcttca
    11701 ggctgataat aatcataata agaaacaaaa tattcaacag cattgttagg gaaaaattcc
    11761 ttgaattccc cgtacagctg accagccaat gttttgttat gggcaataat cagtgttggt
    11821 ttattcactt tctcaataag attggaaatc gtatatgttt tccctgttcc agtcgcacct
    11881 aataagattt gtgctttttt cccgccgact acgccatcca ctaattggtt aatcgcttca
    11941 ggctgatcac cagccggttg gtatttcgag acaagctcaa agtgtcggga ggtatctctc
    12001 tcaatcattc ttcgtcctcc ttttcattca ttccttttca ttttacgacc aataaaaaga
    12061 aaaggaaagc aaaggcattc cttactaaaa aattatacca caccgaacat actttcgcca
    12121 cttttttatc ttattataca tgttatgttt tataacataa aatgttagaa actcgcattt
    12181 ttttcatttt ttcgagctta cagtcttgtc tgattcagca cttttcgcct ataataataa
    12241 aatattggaa atttaggagg ttgattatga aaagaaagca tttatttcac tcatttgttt
    12301 tcttaatgac aggacttgtg acacttgcgt ttggtcatgt tgcacaagct gacaacacta
    12361 caacggcaga gtcaagcaca agcgagacaa tgcaaaaaat agagcccaaa aaagacgtat
    12421 acgtaattgc cagtgactct gcttttgctc catttgaatt tcaaaacgca aagggcgagt
    12481 acgaaggcat cgatgtaaaa ttgatggaaa acatcgccaa actgcaagga tttaacatcc
    12541 gaatggacta ccgtggtttc tctgctgctt tgcaagcttt ggaatcagga caagcagacg
    12601 gaatgattgc cggaatgact gtaacagatg aacgaaagaa aagctatgat ttttctgatt
    12661 cttactttga tagcgggatc caattagctg tcaaaaaagg agatacttcg atcaagtctt
    12721 atgaagattt gaaaggaaaa acagtcggag caaaagttgg taccgaaagt gctgaatttt
    12781 taagcgacca tgaatctgaa tatggctatt ctatcaaata ttttgatgct gctgatcaat
    12841 tgtacgacgg gttaaaagtt ggcgcaatcg atgcaatgat ggatgattat ccggtaatcg
    12901 gttatgcgat caaacaagga caagaattgg aaacaccgat caaaaaagaa tctggcggaa
    12961 aatatgcatt tgccgttaaa aaaggacaaa atcctgagct tcttaatatg ttcaatgaag
    13021 gattgaaaga attaaaaaga acaggcgaat acgataagat tattaaagaa tatgtagcgg
    13081 atggttcaga aacttccagt gagtcttcca ctgctgatga atcaacattt cttggactta
    13141 tccaaaacaa ctatcaagca ttgttgaaag gactttggag aacaattctc ctaactttga
    13201 tctcctttgc tttggctata gtaatcggtg tgatttttgg attattcagt gtagcaccaa
    13261 tacgcgctct tcgcacaatt gctagtattt atgtcgatat catccgagga attccaatga
    13321 tggttcttgc gttctttatt ttctttgggt tgcctggtat cgttggattt acgattcctg
    13381 acttccttgc tggtgttatc accttgactc ttaacgcaag tgcttatatc gcagagattg
    13441 ttcgaggcgg gatcaacgcc gttcctgtcg gacaaatgga agcttccaga agtttaggtc
    13501 tttcttataa tcggacaatg caaaaaatca ttttgccgca agcgatacgc atcatgatcc
    13561 catcattcgt caatcagttc gttatctcat taaaagacac aacgatcatt tccgcaatcg
    13621 gtgtagtcga attgctgcaa actggtaaga tcattgttgc acgtacaaca caaagtacgt
    13681 atgtttactt gatcattgcg atcatgtact tgattttgat cacagcattg acaaaattag
    13741 caaaagtttt agaaaagaag gtgaagtaag atggcagaaa aaatcttagt tgagcattta
    13801 gtcaaaaaat acggcgataa cacagtatta aacgacatca atgtatccat caacgaagga
    13861 gatgtcgtct gtatcattgg cccttcaggt tcaggaaaaa gtacattctt gcgttgcttg
    13921 aatcgtttag aagaaccttc tgacggtgaa attattattg acggcgccca cttgatggat
    13981 aaaagcacag atatcaactt ggtgagacaa catatcggaa tggtattcca acactttaac
    14041 ctattccctc atctttctat tttggaaaac atcattttag caccgatgga cctgaagaaa
    14101 gaatctagag aagaagcaga gaaaaaagca atcaaattac tgcagacagt tggtctagaa
    14161 gataaaaaag atatgtatcc tgaaatgctt tctggtggac aaaaacagcg tgtggctatt
    14221 gctcgtgctc tagcgatgaa ccctgacatc atgctttttg atgagcctac ttctgcactc
    14281 gatcctgaaa tggtcggtga tgtcttgaac gtcatgaaaa agttagcaga acaaggtatg
    14341 acgatggtta tcgttacgca cgaaatgggg ttcgctaaag aagtggccaa tcgcgtcatg
    14401 ttcattgacg acggaaactt tttagaagat ggaacgcctc aacaaatctt tgaaaatccg
    14461 caaaatgaac ggacaaaaga tttcttgaat aaagtattaa atatttaata aaaaccaaaa
    14521 atgcacggtc gccatgaagg gaccgtgtat ttttatcttc ttattttctc acctaatcgt
    14581 atcagccgca tcgcttgttt tcctgcacaa acaagcaatc gctctacctg ttccatcgca
    14641 taagaaagta ccatcggttc attgatgata tcaagtacat agtcgatacc accctcactt
    14701 aattgagctt gatttttctt ctccatatta tgtagttgtg ggacaacttt ctgacaatca
    14761 gcaattgtgt caagaacgga tcaatccttt attggagttg ctcgaaaggg tcttctctta
    14821 ttacggacag gctttgctca ctgtacatag acaacaaatc attatcctag tcaatcggat
    14881 ttccagagcc tcattgctat cattgcttga taaaatccag acaaagttta ataaaatgta
    14941 tcagttacag ctaaactttg gaattgggtc gctttgttat acagagcaag agacaccgca
    15001 atctttcctt catgccaaac aagtttgcga atggatagct ttccatcaat ctgtgaacga
    15061 gatccgcttt tttgaagatt tagacctcgg gatcgtttta ccagcaatcc cgtcagatca
    15121 acgaacatta tatgtaaaac gtattttgaa aagcctgaca gaagaagaag tacatttgtt
    15181 caagaagact ttggcatgct tcagtaaaaa taacggaagc atcaaaaatt gctcagaaga
    15241 attatttatt cataaaaaca ccttgcaata tcgattgaac aagttccatt ctctcactgg
    15301 ttatacccct agaaactatg acgattatca tattctaaag ctggcttttt tgttggtaca
    15361 aacatgacaa ctacaaaaaa accaggcgaa catatgattg ttcgtctggt ttttaattat
    15421 taatgactta gcgtttttac tagaaaaact acttccgctt tttcccgcca aaagcatctt
    15481 taaacttatc gaagaaaccg tcttcttgct gttcttcaac tttgatgccg ccagcttcag
    15541 cgaatgcacg taatgcttct ctttgttgtt catttagatt tttcggtgtg atcagtttta
    15601 ctttgacatg ttggtcgcct gttgcaccac cacgtaactt cggagcgcct tttccacgta
    15661 aacggaagtt cgtacctgtc tgtgttccag cagggatctt caatttcaca tctccatgga
    15721 cagttggtac ttggacttca tcccccaaag ctgcttgtac aaaacttaaa ggcaattcat
    15781 agtaaatttc agaaccttca cgatcaaaga tatcactttc ttctacacgg aagaccacat
    15841 acaaatcacc aaatggtccg ccgttttctc cagcttctcc ttgaccagct aaacgcattt
    15901 gttgtccttc ttctacacca gctggtacat tcactttaac tgaatgtgct ttcttctcat
    15961 gtcctgagcc atgacaggtt ggacaaggtt ctttgatttc tttccctgtc ccatgacaaa
    16021 catcacaagt ttgacggctc atcactcgtc caagtggtgt ttggcgttct acattaatcg
    16081 tacctgcacc atgacattta tgacaagtag ttggttgtgt gcctggtttt gcaccgttcc
    16141 caccacaagt atgacagatt tcttcccgat tatacttgat ttctttttct acaccaaaaa
    16201 tcgcttcttc aaaagacaag tcgaccgtat attgcaaatc agcaccttga cgaggcgcat
    16261 ttggatcgac tgaccgaccg cctcctccaa agaaagagtc gaagatatct tcaaaaccac
    16321 cgaagccgcc accagagaag ccgccgaatc caccaaatcc gccaccagct ccaccagcac
    16381 cgtaatttgg atctgtgcct gcatgcccgt attggtcata tgcagcgcgt ttttgcgggt
    16441 cacttaaaat ctcatatgct tctgatactt ctttgaattt ttcttccgca tcaggttctt
    16501 tattgatgtc aggatggtat tttttcgaaa gcttacgata agctttcttt atttcatctt
    16561 ctgatgctcc ttttgagagc cctaagactt cataataatc acgttttgtt gccataggct
    16621 ttcctcctat tggttctcat cattttcacg tcgcttgtta tcatatcata ttttgtttga
    16681 aacaaaaacc gatacttcaa actatttttc aaaagaaaaa gccaaagtag caactttggc
    16741 cttctctcga aattcagtga actgattatt tatcgtcgcc atcgacttct tcaaaatcag
    16801 catcaacgac atcatctgct ccgccttgag ctgcttctgg gttttcttga gcttgttgtt
    16861 gtgccgcttg ttcgtatagt ttaacagtca agttttgaac gatttcgttc aatgcatcgc
    16921 gtttagtttt catttcttcg atgttgtttg cttcaacagc ggctttcaat tcatcgcgag
    16981 ctgtttctgc ttttttcact tcttcttcgt ccactttgcc ttctaattct ttcaatgttt
    17041 tgtccacaga gaatagcaat gcatctacat cattacgtag gtcaacttcc tctttacgag
    17101 ctttatctgc ttctgcattt gcttctgcat cttttaccat acgttcgatt tcttcgtctg
    17161 ttagacctga agaagatttg atcgtgattt tttgttcttt ttgtgtacct agatctttcg
    17221 cagatacatt tacaatcccg tttttatcaa tatcgaatgt tacttcgatt tgtggaatac
    17281 cacgaggagc tgctggaata tctgtcaatt ggaatcttcc tagagttttg ttgtcagcag
    17341 ccattgggcg ttcaccttgt aatacatgga tgtctactgc tggttgatta tctgccgcag
    17401 ttgagaatac ttgtgattta cttgttggaa tcgttgtatt gcggtcaatc aatttagtga
    17461 acactccacc cattgtttcg atacctaatg acaatggtgt aacgtcaagt aaaacaacat
    17521 ctttcacatc accagtgatc acgccacctt ggatagcagc acccatagca actacttcat
    17581 cagggttaac tgatttgttt ggttcttttc ctgtttcttt acgtactgct tctacaactg
    17641 cagggatacg agtagatcca ccgactaaaa taacttcgtc gatttctgat tgtgataaac
    17701 ctgcatcttt tagcgcttga cgtactggta cttttgtacg ttctactaag tcagctgtta
    17761 attcatcaaa ttttgcacgt gtcaatgtca tttccaaatg caatggtcca gattctcctg
    17821 ctgtgataaa tggcaagctg atttgtgtgc ttgatacgcc agataaatct tttttcgctt
    17881 tttcagcagc gtctttcaag cgttgtaaag ccattttgtc ttgagacaag tcaatgctgt
    17941 tttctttttt gaattctgct accatgtagt cgatgatttt gttatcaaag tcgtccccac
    18001 ctagatggtt gtcgccagct gttgacaata catcaaagac accgtctcct agttcaagga
    18061 tagatacgtc aaaagtaccg ccaccaaggt cgaatactaa gattttttcg tcgcgatctg
    18121 ttttatctaa accataggca agagcagctg cagttggttc attgacgata cgttctactt
    18181 ctagaccagc gatcttacca gcatcttttg ttgcttgacg ttgtgcatca ttaaagtaag
    18241 ctggcacagt gataactgct ttttcgactt tttcacctaa atagtcttct gcgaatcctt
    18301 tgatatattg aaggatcatt gctgagattt cttgtggtgt atatgattta ccttctacat
    18361 ctactttgta acctgcttca ccgatatggc gtttgattga tgaaatcgta tttgggtttg
    18421 tcacagcttg acgttttgca acttccccta cttggatttc tccatttttg aatgatacaa
    18481 cagaaggtgt tgtacggttt ccttctgggt tagcgatgat ttttgcttcg tttccttcca
    18541 atactgctac tgcagagtta gtcgtaccta agtcaatacc gataatttta ctcataatta
    18601 acgtctcctt ctttaattga aagtgtaagt tttatatccg cacaattatt gtgcaacaat
    18661 gaccatggtt ggtcttaaca cgcgatcatg taatttgtag ccttcttgta gtacttctac
    18721 aatcgtatcg gctggtgtat cttcagtcgc tggaacagtt tgtactgctt gatgaagatt
    18781 aggatcaaag gcttctcctt tagcagggat tttttcaatt ccttcttctt ccagtgcgtt
    18841 acgcaaactt tccaagacca tttccacgcc ttttttcaaa cctgcccctt gatcatctga
    18901 cacttctgtg gctaatgcac gttccaggtt gtcaattgat ggaagtaatt ttttggctaa
    18961 atcttgtgaa cggtattttt gtaattgctc acgctcattt tttcctcgat ttgccatatt
    19021 tgcgatttca gcacgagccc gcaagtattt atcttccatt tcttctaact cagcttttaa
    19081 tttcgctgtt tcgaattctt ccgcttcagc ttctgaaata cccgaatctt ctgcagcttc
    19141 gacatcgatt tccggttctg gctgagcatc tgtcatttct ttatctagtt cttcttgatt
    19201 ttctttcacg atacagtctt cctttcatta ttcagtcagc tgtttgattg ctcttactaa
    19261 gaaagagaga acgatcagct gcaaatagct tcgttattca cattacgagt ttgacaagtc
    19321 aagcgaacgg tagtaatcag caagtttagc tgctagttct cttcgataaa catcgactag
    19381 accgaacatc cgagaataag gcatactagt aggccctaac aaggctattg tacctcttcc
    19441 atgaccatct atctcgtaac ttgcttgaat catactcata ttctgcaata aatcattgcc
    19501 taattcagca ccaatcttga tatgaatatt gctatccatc ggagtgatca tttgtgtaag
    19561 tccgtctgag tctttcataa aagaaaacat cgatttgaac tgatcaagat cttgacgggg
    19621 ctcataatcc aagagattca tttgtccact gacataaacc ttatcttcaa aaacatgacc
    19681 aagaacggtg tcaaataact gagacatccc ttctgtcgtt tggaaatatt tatgaagaat
    19741 catcggaatc tccgtacgaa gtttatggta caccgtcatc agaggatctc cgactagacg
    19801 gtcgtttaca atctgtacca ttttttctag ttcctgactt cccatatatt cgggaagagt
    19861 gaacacttgg ctttctacat tccccatatc tgttacgacg atagcaataa tctgtcgatt
    19921 attcaagggt acgatacgaa agccagtcag tcggcggtct tttatttcag gtcccaatga
    19981 aaaagtcgta tagcttgtca gctcagataa tatctcagca gattgcttaa taatctcatt
    20041 gatttcatga aattctctgc ctagagactg acgaatcagc agcaaatcat cttctgctac
    20101 gcgagcaggt tttaacaaat gatccacgta ataacgatag cctaaagcag atggaatccg
    20161 cccagaagaa gagtgagtct tcaacagcaa tccttcatct tctaatgctt tcatatcatt
    20221 gcgaatcgtt gctgagctgg ccttgactcc ctcagacatc aatgttttgg aaccaacggg
    20281 cacgccacta ctcgtatagt gttggatgat cagacgtaaa atgtcgtttt gtcgctttgt
    20341 taacatcttc ctcacctctc attagcactc atatattcta agtgctaatc cacttataaa
    20401 tatagcaaga agcccttctc ctgtcaagag aaagactcca ttttttagca ctctaaacac
    20461 aagagtgcta attctaaata attgatgtaa atc
//
LOCUS       DASJST010000039        20285 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJST010000039 DASJST010000000
VERSION     DASJST010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.039"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..3437
                     /locus_tag="U4B05_001172"
     CDS             48..3437
                     /locus_tag="U4B05_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2050324.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3440..4861
                     /locus_tag="U4B05_001173"
     CDS             3440..4861
                     /locus_tag="U4B05_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2050325.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4858..6735
                     /locus_tag="U4B05_001174"
     CDS             4858..6735
                     /locus_tag="U4B05_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2050326.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6827..7669
                     /locus_tag="U4B05_001175"
     CDS             6827..7669
                     /locus_tag="U4B05_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2050327.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7821..8696)
                     /locus_tag="U4B05_001176"
     CDS             complement(7821..8696)
                     /locus_tag="U4B05_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2050328.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8903..9934
                     /locus_tag="U4B05_001177"
     CDS             8903..9934
                     /locus_tag="U4B05_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2050329.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10023..10328
                     /locus_tag="U4B05_001178"
     CDS             10023..10328
                     /locus_tag="U4B05_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN2050330.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10507..12522
                     /locus_tag="U4B05_001179"
     CDS             10507..12522
                     /locus_tag="U4B05_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2050331.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12524..12808
                     /locus_tag="U4B05_001180"
     CDS             12524..12808
                     /locus_tag="U4B05_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2050332.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12834..14207
                     /locus_tag="U4B05_001181"
     CDS             12834..14207
                     /locus_tag="U4B05_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2050333.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14290..16305)
                     /locus_tag="U4B05_001182"
     CDS             complement(14290..16305)
                     /locus_tag="U4B05_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN2050334.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16327..17541)
                     /locus_tag="U4B05_001183"
     CDS             complement(16327..17541)
                     /locus_tag="U4B05_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2050335.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17762..18706
                     /locus_tag="U4B05_001184"
     CDS             17762..18706
                     /locus_tag="U4B05_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050336.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19002..19946
                     /locus_tag="U4B05_001185"
     CDS             19002..19946
                     /locus_tag="U4B05_001185"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2050337.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6933 a         3723 c         3865 g         5764 t
ORIGIN      
        1 tttcactatt cacgtcaaaa cagaaaaaaa gaaaaaggag gggaaaaatg acgaccacag
       61 ggaagaaact gaaagttatt ttcatgctga taatattgag tttatcaaac tttgtgccat
      121 tatctgcaat agcagacact acagatgatc caacagtttt agaaacaatt tcagctgaag
      181 tcatttcgga tcagtctgga aaaaaagcac tgaacatcaa gctaaatgcg aataacacca
      241 gtgctgaaaa gatagaaaaa gaaattggtc tagtcgaaaa ttacttaagt gatgtggaaa
      301 gaaaagaagg agatggctat gcttatcagg taaatagcgg gaaaattacg ttggaaatct
      361 catcaaacac taaacaaact atcgatctga gttttccaat cgatccagca ctttaccaca
      421 gccaggcaaa caagctgatc gtcgataata aagaatatga cattattgat gagacagaaa
      481 ataagaaaga tacagatgtg tcagtaccaa agccagacga aatagaagaa gaatcatcaa
      541 aagaaaacga aaattctgtc agcccattta cattgcctac attatccttg ccagctgtga
      601 gtgtgccatc taatcaaacg attcctacag aatatacaac agatgatcag ggcacttatc
      661 ctaaagccag ttggcaacct acaggaaata caaatgttct tgatcatcaa ggcaataaaa
      721 acggaacaaa tcaatgggat ggtataaatt cttggaatgg agatcctaat gatcggaccc
      781 attcgtatat cgaatatgga ggaaccggta atcaagcaga ctatgcgata cgaaagtatg
      841 caaaggaaac aagtacaccc ggattgtttg atgtttattt gaatgctcgt ggaaatgtac
      901 aaaaagatat cacgcctctt gatctcgtat tggtcgtaga ctggtcagga agtatgaacg
      961 acaataatcg gatcggtgaa gtaaagattg gtgtcgatcg ttttgtcgat actttagcag
     1021 atagcggtat cacagacaaa atcaatatgg gatatgtcgg ctactcaagc gaaggatata
     1081 gctacagtaa cggtgcagta cagatgggtt catttgattc agtgaaaaat caagtaaaat
     1141 ccattacacc ttcacggaca aatggtggta cttttacaca aaaagcacta agagatgcag
     1201 gaagcatgct atccgttcca aatggacata aaaaagtgat cgttttgctg acggatggtg
     1261 taccaacatt ttcctataaa gtacagcggg tacacgcaca atcaagcagc aattattacg
     1321 gaactcagtt ttctaatacg caagatcggc cgggaaatac ttctctaatc tcaagaatct
     1381 atgatgcacc tgaccaaaac aatctatcca gaagaatcga cagtacgttt atcgcaacca
     1441 tcggagaagc gatggcactc aaagaacgag gaatcgaaat acatggtctt ggcatccaac
     1501 ttcaaagcga tccggcagct ggtctctcaa aagcagaagt agagtctcgt atgcgacaaa
     1561 tggtttcatc agatgaaaaa ggcgatcttt actatgaatc agctgatcat gcaacagata
     1621 tctctgaata cctagccaaa aaagctgtac agatctcagc aactgtaagc aatggacaaa
     1681 taaatgatcc aatcgcagaa ccattcattt atcagcctgg tacactttca gtcaagagtg
     1741 tggggacaag tcctacaacg gtcactccat ctatttccat agaaggaaat accatcaaga
     1801 gcaatcagat ctatttagga aaagaccaag aaatccaaat ccattaccaa gtgagaatcc
     1861 aaacagaaaa tgaggacttc catccaaatt tctggtatca aatgaacggc aggacaactt
     1921 tccagccaaa cattgatacc aatgaattag ctgaattcgg tataccatct gctaaagctc
     1981 ccggagtcag tcttcacatc aaaaagttat gggaagaatt tgacaacaat ctagctgatc
     2041 gtccagatca agttactttt gagattcaac gggaacatac gacaaatgct gcagcttgga
     2101 aaaacggata tattcgaatc attaaaccag ctaaagatac aacaaatacg tgggaacgtg
     2161 cagacattga caaattatct gcaaatagcg gagaaagtta tcaagagata ttatcactac
     2221 ctcaatacaa taatcaaggt caagcattca gttaccaaac aatcaaagaa ttacctgtac
     2281 caggatacga ttctcaacaa atagatgcaa tgacatggaa aaatactaaa caattcacac
     2341 cgttaaactt gaaaataacg aaaaattcct ctacaggtga aaaggatctt attggcgctg
     2401 ttttcaaatt aacaggagat tctattgata ctttactaac agatcatggc gacggaacct
     2461 attctcttcc agaaaatgtc aaattgcaaa aagaaatgac ctatacgctg acagaaacaa
     2521 aagctccaga agggcatgga ttaagcaaaa agactacttg ggaaatcaag atcgcttctg
     2581 atggtacggt aaccattgat ggaaaaacag tcactacttc cgatgatacg atccagttga
     2641 ctattgaaaa tccttttgtt gaagttcctg tagcagtacg taagtatgcg atgcaaggga
     2701 cggacaaaga gataaatctt aaaggagcag cattttccct acagaaaaaa gaagcaaatg
     2761 gtacttatca gccaattgac agccaaacaa cgaatgaaaa aggtcttgcc agttttgatt
     2821 cactcacacc tggtaaatat cgagtcgttg aaacagctgg tcctgccgga tatgatactt
     2881 cgccgggaaa ttatgaattc caaatcgata aatatggaaa aatcatttac acgggaaaaa
     2941 ataccgagat gacaaataat gtatggacgc tcactcatca aaatcgacta aaagcgtttg
     3001 atctaacggt acacaaaaaa gaagacaacg gacagacatt aaaaggagca aaattcagac
     3061 tgcagggacc agaaatggac ttagaatcgc caaaagatgg acaagaaaca gatacctttc
     3121 tattcgaaaa tttaaaacct ggaacttata cgctgaccga aacttttaca ccagaaggat
     3181 accaaggtct aaaagagcca gttactatag ttatacacga agatgggtca attcaagtgg
     3241 atggacaaga tcatgaatct gttctgtcac caggagccaa aaacaaccag atttctttag
     3301 acatcacgaa tcaggcaaaa gtaccattac ctgaaacggg aggaattggc cgtttaggaa
     3361 tctatctagt agggatgatt ggttgtgcgt tttctatttg gtatcttttt ttgaaaaaag
     3421 aaagaggggg cagctaaata tgaaaaaact tggttggctt agtatgtgtc tcttcttgtt
     3481 actatttaaa ccagctttta ctcaggtagc aacagaaaca gaaacagaaa tggttcagat
     3541 tactttacac aaattgcttt tcccaaacgg gcaactgccg aaaaatcatc caaatgacgg
     3601 acaagaaaaa gctttattac aaacgtatcg aggattaaat ggtgtcacat tccaagttta
     3661 tgatgtcaca gattcttttt accatctacg ggaaaagggc aaaacggtag aagaagcaca
     3721 agcagagatc gcaaaaaacg gtgcgtcttc cggtatgttt accgcagaag caacaactac
     3781 aactcttaac aacgaagatg gtatcgcttc tttttctctg gccgctaaag atcaagaaaa
     3841 aagagataaa gcgtatcttt tcattgaatc caaagtacca gaagtcgtca aagaaaaggc
     3901 agagaatatg gtagttgttc ttcctgtaca tggacaaaac aatcaaaaac tttcaactat
     3961 ccatttgtat cctaaaaatg aagaaaacga ctaccctgat ccaccttttg agaaggtatt
     4021 agaagagcct agaaatgatt ttacgattgg tgaaaaaatc acttattcct tgcatacgac
     4081 aattcctgta aatatccttg actatcaaaa gttcgaattg tcagatagtg cggatgaagc
     4141 attaacgttt ttacctaata gtttaacgat ttcatcgaat ggagaaaagc tgacagaagg
     4201 ctttgtcata cacaagaaac ctcacggatt tgatgtttta ttttcgatcc cttcgttgga
     4261 aaaatatgct ggaaaaaaac tgaccatttc ttatcagatg cagctaagca gtacagcaca
     4321 ggcgaacaag gaaatcaaca acaacggaac actggatttt ggttttggtg tcagtacaaa
     4381 gaaagtctct gtatatacag ggagtaagca atttgtcaaa atcgagacaa ataaaccaga
     4441 taaacgatta gctggcgcag tattccttat taaaaacaaa gcaggaaatt acctccagca
     4501 aacagccaac ggatacaagt ggacaaagaa cgaatcagat gcgcttcacc tgatttccga
     4561 taaaaatggc gctttttcaa tttccgggtt gaaaacagga agttatcgat taaaagagat
     4621 cgaagcacct tctggttata ttttaagtga aacagaaatt ccgtttacca tttcaacttt
     4681 tctttctgag gataaagagg cggacagtat attgaaagta gtcaataaaa aagaaaatag
     4741 ccgtccattt cttccaaaaa caaacgaaac gaaaaataca cttttaggcg ttgttggtat
     4801 ggtattcgca agctttgcaa tctggttgtt tatcaaaaaa agaacaggag tgaaaaaatg
     4861 aaaaatcata aaaaaataaa cgttatgtta ggagtccttt tccttatttt accattactc
     4921 acaaacagct tcggcgcaaa aaaagtgttt gcagaggaga cagcagctca agtcatcctt
     4981 cataaaaaga aaatgactga tttacccgat cctttaatcc aaaacagcgg gaaagaaatg
     5041 agcgaattcg atcaatacca aggattagcc gatatttcat tttcagttta taacgtcact
     5101 caagaatttt atgcgcaacg agataaagga gcgtccgtgg atgcagcaaa acaagcagtc
     5161 cagtctttga ctcctggtac accagttgct tcaggaacga cagatgctga tggaaatgtc
     5221 actttatctt tacctaaaaa acaaaatggg aaagatgcag tctacacgat caaagaagaa
     5281 ccaaaagacg gagtgtcagc tgccgcaaac atggttttag ctttccctgt atatgagatg
     5341 atcaaacaag cagatggctc ttataaatac gggacagaag aactagatac tatccatctc
     5401 taccctaaaa atacagtcgg taatgatgga acgttgaaag ttacaaaaat cggtactgcc
     5461 gaaaacgaag cactaaatgg agcagaattt attatttcta aagaagaagg aacaccaagc
     5521 gtcaaaaaat acatccaaag tgtcacagat ggattgtaca cttggacaac tgatcaaacc
     5581 aaagccaaac atttcattac tggtcattct tatgacatcg gcaacaatga ctttgccgag
     5641 gcatctattg aaaaaggcca gttgatcgtt aatcatttag aagttggaaa atataattta
     5701 gaagaagtaa aagctcctga taatgcggaa atgattgaaa agcaaacaat cacgcctttt
     5761 gagatcctgg caaatagcca aacaccagta gaaaagacca tcaaaaatga tacgtctaaa
     5821 gttgataaaa caacacctca attgaatgga aaagatgtcg caatcggtga aaaaattcaa
     5881 tatgagattt ctgtcaatat cccattaggt atcgctgata aagaaggaac gcaaaacaag
     5941 tacacaacat tcaaacttat cgatactcat gacgctgctt taacatttga taatgattct
     6001 tcaggaacgt atgcttatgc cttatatgat ggaaataaag aaatcgaccc agtaaattat
     6061 tctgtcactg agcaaacaga cggattcacg gtttcagttg atccgaatta tattccttca
     6121 ttaactcctg gcggtacatt gaaattcgtt tactatatgc atttgaacga aaaagcagat
     6181 ccaaccaaag gattttctaa ccaagcaaat gtcgataacg ggcatacaaa tgatcaaaca
     6241 ccaccgtcag tcgatgtcgt tactgggggc aaacgatttg ttaaagtaga tggtgacgtt
     6301 acatcagacc aaacacttgc tggagcagaa ttcgtcgttc gtgatcaaga tagtgacaca
     6361 gcgaaatatt tatcgatcga cccatccaca aaagccgtca gctgggtatc ggcgaaagaa
     6421 tcagcaacgg tttttacaac cacaagtaac ggtttaatcg atgtgacagg tctaaaatat
     6481 ggcacgtact atctggaaga aacgaaagcg ccagaaaaat atgttccatt aacaaaccgt
     6541 gtagcattta ctatcgatga acaatcttat gtaacagcag gacagttgat ttctcctgaa
     6601 aaaataccaa ataaacacaa aggtacactt ccttcaacag gcggtaaggg aatctatgtg
     6661 tatatcggtg caggagtagt ccttctactg attgctggac tgtactttgc tagacgcaag
     6721 cacagtcaga tttagtcttt ttgcaagaat tggaaaaagg agcagcggat tacgcaaatg
     6781 ctcctttttc ttttattatc aaacagtcat cacaaggaga acacaaatga aaccaaagaa
     6841 aaggaaactt ctagatctgc tgatgctttt cttgcttctc tcaggaatag ggatactagc
     6901 ttatccattt gttagcgacg cgctgaataa ttatctggat cagcagatca tctctcatta
     6961 tcagcaacaa gcggtcaaag aaaatgaaga agtcatggct aaaatccaag aaaacatgac
     7021 aaagaaaaac cagcaattag caaaagaagg tggaaatcca ggtgcagatc cctttactaa
     7081 gaaaaaagaa cctgcgaaaa aggatagaac ctattttgag aaacatacga tcggtatttt
     7141 gacgatccct gagatcaatg tccatttgcc aatttttgat gaaacgaata ctgtgctgct
     7201 agaaaaaggt gcctcattat tggaaggaac gtcttatcct acggggggag aaaatacaca
     7261 tgccgttatt tccagccatc gaggactgcc tcaagcaaag ttgttcacag acttaccgaa
     7321 actgacttta ggtgattctt tctatatcga aatcaatgga cggacactcg cttatcaagt
     7381 cgatcagatc aagacggtag aaccgactga tacaaaagat ctgagaatcg aaaaagggca
     7441 ggatttcgtg acactcctaa cttgtacacc ttatatggtc aacagtcatc gcttacttgt
     7501 cagagggcac cgggttcctt atcatgcaaa agaagttcaa aaagaagtac agaaagtatc
     7561 cgagaacaaa cgatttttcc tgtatggatt gttagctgca tgtatactga ttttcctcct
     7621 tcttatatat ctttttcgtc aatcgttcaa aataagaaac agaagttagc tattcctcat
     7681 ttctataata tatctattcg aatcgttccg taaacaaaca ggctgaggtt tgaaaaagaa
     7741 aacttcagcc tgtttttatg atgatatcaa agagagccta tcaaacacta tctttcttat
     7801 tactaaatga ggatcttttc ttaagttttc cgatattctt taagaaggcc tttttgcatc
     7861 aattcttgtt tgtttttcaa gttgcggata ttctcttcaa aattttgaga atatatcact
     7921 gagaataacg tatttaatac gtaatcgaag gcaatttgag aggagaaagt cgctacttta
     7981 gcaaaatcat attcctcctg cgatataaca agacacatat cagcctgttt ggccatttct
     8041 gaatgctgat ttccggtaat cagcaaggta ggggcctcta catgcttcaa ataggtcatt
     8101 attcgttcat aatgatggat gcgaccgctg tagctaataa aaatggcgca atcctcgcta
     8161 gtcaaattgg ctgcattcca agaagaatca ctgtattctt cagccatgat caaaaactta
     8221 ttcaacttga ccattttatt ctggaatttc cttgccgtga tttgagaatc accttttgca
     8281 aaaagaaaga ttcgctgtgc cttgttcaat actttaccga tttgatctaa atcttcatgt
     8341 tcaatctgca attgtgcttt ttttattgac tggatcgata aatccgccat tttctttgca
     8401 atttgctgag tgctatcttc acttgaaaaa ggaaaattag gatcaatcgt ttgattgaac
     8461 tgagcaatca attgatgaac ttcccgcccc agctcgaaac ggaattcttt aaatccgctg
     8521 aatcccattt ttttgcataa acgaatgata gctgaatggg aagtatacgt agcttttgct
     8581 aattcttcaa tcgtcatgta gactgcagct tcgatattcc tacggatgta atcgacaatc
     8641 cgtttctctg tcgtggtaaa caactcttgt tttgttagtt tttcttcaat aagcatgatc
     8701 tgacctccaa attctatctt actgataaac acttggttcc gctactatct ttttgatttt
     8761 tatccttact ttgaacatta atttcaccaa accgaatgat aacaagtgtt tttctgaact
     8821 tttttaccag aatagctatt ataaatactt tctgcgcttt tctaggatat actgaaaaca
     8881 tcaataaaag gaggcaccac tcatgcttac aatcgcttat cttggcaatg gaaagagtac
     8941 aaatcgctat catttgcctt tttcaagaaa acttacgaac aaaatcaata tcaaaaaaat
     9001 tttttcccca tctgaaccag tgtggaaaac attcgaagac atcgaatata ctgatcagct
     9061 gtctgatctt tgggatgatc ctgagatcca attagttgtc atcactacac ctagtcaatt
     9121 tcattatgaa tatggaaaaa tggcactcga acatggtaag aatgtactag tagagaaacc
     9181 ttttgcagaa acgtcaaagg aagcagaaga gttatttgca ttagcaaaag aaaaaggtct
     9241 atttatccaa tgttaccaaa acagacggtt cgattctgac ttcttgacag tccaaaaagt
     9301 aatcgaaagc ggaaaattag gggatatttt agaagtagaa atgcattatg attatttcag
     9361 accggaagta ccagaaaata cgcatgaatt ttctactgcg aacagctatt tatatggaca
     9421 tgcttgtcat acagtagatc aagttctttc ttatttcggc gaaccagatg atatcagcta
     9481 tgatgtacgt caacttttag ggaaaggacg aatgaatgat tacttcgatc tggatttttt
     9541 ctatggaaat atgaaaattt cgataaaatc cagttatttc cgcatcaaag aacgtccaag
     9601 tttcgtcgtt tacggaaaaa aagggatgtt cgtgaaacaa acaaaagatc gacaagaaga
     9661 acatctgaaa atgttttaca tgcctgatca tgaagacttt ggtatcgatt tgcccgaaca
     9721 ttatggtgta ttgacctata tagatgaaca agggagttat catgaagaaa aagtaatttc
     9781 cgaggttggt gattacagcc gtgtctacga agggatttac gagacattag ttaatggcgc
     9841 agaaaaagtc atcaaagatg aagaaacaat cttacaaatg aaaattttgg aagaaggaat
     9901 caaaagcatt caagaatcag aaggaaaaat atgacatatt tgtaagaatc gggtttgttt
     9961 tttgacgcat aaactgtctt attgtacgat gaaaaagtat agaactacta ggaggaacca
    10021 acatgacaaa ttatgacggt gaaaaacaca aacgattaag aggttcaatg gcccctaaaa
    10081 cagaagaaga agtggcttta gcaggaacta attctggtga aacagaagac aaagaagaat
    10141 cattagctgg cacaaactct ggtgaaacag aaaataaaga aacagcttta tctggttcaa
    10201 attctgtaga agaggaaaca aagcctaatg agcccaatca ctttgaaaaa gatatcgaaa
    10261 aagcggttga agatgtcaaa gaaaaatttg aagaactgac ctcaaaaaat aaacatgatc
    10321 atcattgatc ggacgaaatc aattcataaa agcaggagac atttggtcac ctgctttttt
    10381 tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa aaaaaatcca actgtaagtt
    10441 gtaccaaaca cccctcgatt taaaagcgct accatactag aatataagta ggaggtgaac
    10501 agcgctgtga actacaaaat tagttatctc ctgaaaacac caagagaaat cagtctagaa
    10561 aaagcagcag agatcatcga agtaaataca agcgaggcga tcaaacaatt aactcctttt
    10621 gccgaatatg tggatgatac ccgtatccgt ataaagaaat tccgagcaat cgactgggca
    10681 gaaaaatttt tggatattac tcgatttgaa gctgtgtata ccgaacaaga gcgaagagat
    10741 cttctttatt tgatggttta ttcgactgca acgtctgttt ctgtttttca ttttcaagat
    10801 tttcttcagg tttctaaagg gacagtcctg tcagatatca aaaagataag aaatgaatta
    10861 gaagtggaag attgtcagct gtcgtatact aggaaaactg gttttaggct gctagggtca
    10921 gaagcaatat taagaagaga agcaaaaaat cgcactgctc gtttgatgca gtcacttgct
    10981 ggaagatttg ggctcacgta tttaaccgca accctttcca ttgattatta cgccaaagtt
    11041 agagatttgc tagataaaac gatttcacta tcagatcttc gattcgcacc aaatcgaatg
    11101 gatgaattag cttattattt gagtttgaca accattcgaa ttggaaaaac atccatggaa
    11161 ctggtaaaag atgctgaact gttggcttct cttgcagtca ctgacattag tcgacaagta
    11221 ttgactggcc ttggctattc cgaacatcaa aattcggaga ttatattttt ctctctttgc
    11281 ttagcatcga tcgttgaagg agatgtccag gagcctgcac ttgatttttt gctggaatgt
    11341 tcttcagaag tcatccatcg catggaattc ctgatggccg tagaatttgc ttcgtttcgt
    11401 gaattattga tgaatgtctt ttatcacttg gtccctgcct attttcgtat ttcctattct
    11461 ttttatttac ctaatgtgat gatcgatcaa attaagcatc aatatgcttc catctacgaa
    11521 atgacaagaa aagcattact tccgttagaa agacgaatag ggaagagcat ccctgaagaa
    11581 gagattggtt ttttcactat actatttggc ggagagatca gaaaagcgga cgcagaagaa
    11641 agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg gcattagctc ttcattgatc
    11701 ttaaaaagtg aacttcagca attattcccg atgatctttt ttacagagac taattcagct
    11761 gaccgcttac acgaggttga cgaaacttct tacgatatta ttttttctac tgtacctctc
    11821 acacttactg ataagaaaaa gtctctctat gttctgcatc caatcatgac tgacttggaa
    11881 aaaaatcagc tgatcaatca agtacaaaga gattggctga taccaggatt ttcaatgcct
    11941 tctgcccaag aattgttgtc ggcgctactg ccttatatcg aattaaaaga aggcgtagac
    12001 gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca aacttttaga aaagaaggag
    12061 gatcaacgtc ctatgctatc tgaattatta acagaagaaa tgatccaatt atcggatcaa
    12121 ccaaaaaact ggcaagaagc aatcacattt gccgcacagc cgctattatc agaaaataaa
    12181 atagaagaaa gttatatcaa agctatgatc gagcgcgtgg agcagtatgg tgcgtttatc
    12241 catattggcg atcacatcgc attacctcat gctcgtccag aagatggcgt aaacgaagta
    12301 gggatgtctt tgctgaaact tcaacaacct gtggatctgc ttgataatcc agcccatccc
    12361 attagtttgt tcatctgttt agctgctatc gataatgagg cacatcttcg ggctttggca
    12421 aatttaacaa agctactttc taataaacaa aatcttcaag atttattaca cgcatcaaca
    12481 aaagaagaaa tattagcgat cataagaaaa ggagacgaat gatatgaaaa tttttgcagt
    12541 atgtcaaagt ggattaggaa caagttttat ggtagagatg aacatcaaac aagcacttgc
    12601 agatcttgga gcagatgtgg acgagtttga agtaagtcat acggatgttg gaagtgtatc
    12661 tagcgatatg gctgattatt tcttcattga gcattctttg aaagatacgc taacttctat
    12721 acctgatgaa aagctcgtac ctttgcaatc gatcattgac agcgaagaag taaaagaaaa
    12781 agtaacgaag atcttgtcaa aagaataaaa atgattcaag ggaggaacta tcaatggata
    12841 aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc catcctcgtt tctttgattg
    12901 cactgcttgg tctcgttctt cagaagaaga aattttcaga agtgatccaa ggaactatca
    12961 aaacatttgt cggattccta gtactgatcg gcggtgctgg tttaatcagt aattcactaa
    13021 ccccttttgc ttcgatgttc caaacagctc tgcatactca aggagtagtt cctagcaatg
    13081 aagcagtagt ggcgattgct ttgcaacaat acggtacacc tactgctttg atcatgctag
    13141 tagggatgat cgtgaatatt ttacttgcta gattcacaag atataaattc atttttctta
    13201 caggtcaagc aatgctttat gtttcttgtc taacagcagt cattttagta agcgcggggt
    13261 ttactgcaaa tttgtggatg atcttattag gcggggtctt cgaaggaaca ctattaacta
    13321 ttacgcctgc tctttgtcaa ccgtttatga agaacatcac tggaaacaat caagttgcgt
    13381 tagggcatac cggaaatatc ggttatgctt ctagtggatg gatcggaaag ttttttggaa
    13441 acaaagaccg ttctacagag gacataaaga ttcctaaaag tttcagcttt ttgcgagatt
    13501 ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt cgcacttgct ttgttagctg
    13561 gtcctggatt tgttgaaaga gaactaagca atggagacaa cgctgtaatc tttgcactga
    13621 tccaagctgg aacattcaca gctgggtttg ttatcgtcct tcaaggggtt cgaatggtgt
    13681 tggcagaaat cgttccagca ttccaaggga ttgctaaaaa actcgtaccc gattctaaac
    13741 cagcattaga tgttcctatc gtttttccat atgcacctaa tgctgtatta attggctttt
    13801 ttgttagctt ctttgtagga acactatcca tgtttgctat gataggcttg caaactacag
    13861 ttatcattcc tggggtagta ggacatttct tctgcggagc tgcttctggt gtcttcggca
    13921 actcgactgg cggtagaaga ggggcggtat taggttctgc tttcaatagc ttattgatta
    13981 gttggctgcc attatttatc cttccagtat taggagatct taaaatcgct tcttctacat
    14041 ttgccgatac tgattatcta gtaccaggac ttgtacttgg gaaactagga gaatacggtc
    14101 aaatagcctt gacagttgga atcatcgctt tctttttact tgtcgtatta tctagctttc
    14161 tttttgccgg aaaaagaaca aacattgttt ctaaagaaca gaattaagca aacttcaaaa
    14221 taaaaattgc ttggacaata tcagaagaag atttaaagtc ttctgatatg tcgcaagcag
    14281 ttttttgttt tatgcattta tttttgaata gcttgttaag acaagtggct gttttgctag
    14341 aagaggataa cttttaccat tcattttcac tgttccgcct gttctatcag aagtaatcac
    14401 gatcttatct tttgtttttt caagcgaaat ctttccatct ttgataggta gcgtacaatt
    14461 cagtgtttca aatgaatcta gattaggttg tatcataaat tcttcataac ctggctttgt
    14521 tggatacaaa ccgataaaat agcgtcctaa aagatagatt gggcttgctc cccatgcatg
    14581 gcataagctt tttccataag gatctccata catactgtac ttttcatttc ctgtttgagt
    14641 agggtcgtat tcttcccaga aagttaccgc atcatgagat aacatccctc cccaataatc
    14701 aagaatcgtt tggtagacga tattcgtctg tccgagtttg cataaagcat cttgttcata
    14761 gaatttgaaa tatggagtag taatctgtgt gatagtgtca ttcaataaga cattctttaa
    14821 gatcgattgc tgttggtctt tgttagcaaa atcgaataaa acagcgaaaa tattcgcatg
    14881 acgcgtcaca tttcttctcc cagattcata gcaatcgata aaagcgccaa gttcttcatc
    14941 ccaaaagtag tcaaataggt tttgtttcaa tagttgatat ttttcttgat agagatgagt
    15001 atgataattt tcgttctctc catatgtttc agctaagata tctccgcaag tgatcatgga
    15061 atgataggct ttcagtaata agacttgttc tgcagcaagc gttccttctt tatccatttc
    15121 agaccaatca ataaatatcc aatcttttgc tctcccataa ataaacccta attcattcgt
    15181 ttgctccatc aagtatctca ttaatgattg catcttaggg tagacgatgc gtaaaaattc
    15241 atagtctttc gtcatcatat agtggttttc gataccaatc aaccacaaga tcgaatagtc
    15301 aacgatagta ttcatatgct gcttgatttc attttgtcct cttaatgcta atatcgttcg
    15361 tttattgatt tcctcgtcaa aaaaaagata ttgattgatc atgaatgatt gatacgcatc
    15421 tccagaccaa atccagcgat ctcgtttgat accgtcgata aaaaacaaat cactacataa
    15481 cttgaaggct tcttctgata ctgaccagat ctgattcagt aaagaagaat cgcttgaaaa
    15541 agcagcttgt ttcgaaagag ataaaaattc atgaaaaact tgaagttgaa ccattccagt
    15601 agaaacctct ggtataaaca aatagcgaaa agcgcgttta cgagctttca tttttcttgt
    15661 cattcctatt tgcttatagt agcaataatc gatatccata gcttctacat cggactctcc
    15721 ataacataga gtgatagatt cgatatggtc atccagagat attagttcta cacagccatt
    15781 cactgctcgc ccaaaatcga ataagattcc tccgccaatc tccgtttcag tgactggatg
    15841 gacgcattct ttttgataat agatttgatt tggatctttt gcgatatcga cataaagcgg
    15901 actccagcca gctggatatt cttcaataaa attacttgct gtccagccaa tatctgattt
    15961 tacttcgtct ccctcgataa acatcgctgg tagccctgat gtatgtccta caaagatacg
    16021 aatttttgct ttaccagctg gacaagtcag ccattttcca catggatgtt tttgtccatt
    16081 aatttctaca tacccaactc cttggatcgt cactttaaac atggaaggtt gatcaagaaa
    16141 gtaatatctc ttaaacttga cgttccgatg gcaatcatcc atgtaccagt acgctggcca
    16201 gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt ccatggcgga tctcaaagtc
    16261 tccaggatac caaagccatc tatctttatg tttttcattt gtcattttac acgacctcca
    16321 tcaaatttat tttgttgtca attgttcttt tatttcttca tctactctta tcactgtctt
    16381 tctattcgtc ctgtgtacta ctagaaggct aataagcgcg ctaatcaaca gcaagatact
    16441 gccattgacg aaaacagcaa gtgttttact cccaagagaa gcaaaagaga gaaagagcaa
    16501 accatatggt gcaatgaaag tacctaaatt agcgccaacc aataagattg aagtattgaa
    16561 actctccgcg tacttgggcg cttggatact tacttggttg aataagtaag gaatgaataa
    16621 cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc acatacaggt ttatcacagt
    16681 ggcaatcaat acaaagccga ttcccatcag tagtaaagaa agtgtaagca tatagcgatg
    16741 tgcttttgtc tgaagccagc caaaacaaac tccggcagcc atacttgcca attgcatgat
    16801 tgttaagata ttcgctgcat tagtttcgtt tcctagagaa taagtcgtca ggactgtggc
    16861 taatttaatc gatacagcat tatataccac gatgaccagc aagagaaaaa taccataact
    16921 aatcatttta ggattcgttt tgattacttg tcttgttggg gctgtgccat gcgattgaag
    16981 ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag acaagaatag gtaagctgat
    17041 ggcgtagact aaaaaggtat attgccaacc gaactgtact agctgtcctg caacaaatgt
    17101 cattaaagct gcaccaaatc cttgaaatgc actttgaaaa ccaatcatag aggctcgttc
    17161 atctccgtga taaaaggcag caatcatgct gactgccaaa gaattgatca atccaaagcc
    17221 aatacctagt ccagctcttg aagctaagac aatactgtaa ctttgtgaga agagggggag
    17281 gataccacaa atcgatgcaa tcactaatcc gatgatcaca gtttgttttt gtccgatttt
    17341 cctagcaata aaactactaa tcaaaacaaa aatagtgacc ataaaagagg gagcagtcgt
    17401 taataattca acagcagata aagaacgcgt ggcataagta gctgacatac tgggaattgc
    17461 agacgtgact gcaccagcag aagtcaaaat aagagagact gacagcagag aaaatttcat
    17521 catcttttta ttcataagca tgttcctttc atttttgtaa gcgcttatca aaaatatagt
    17581 atgaagtggt tattctctca atagcggaat cacaggaagt tcatgtccca accatagaaa
    17641 gtctttatga aaccaaaaat agctttgcat ataatagcag tacagcgtga actaataagc
    17701 gaaagaaacc gcttacaaaa aggagggagt caattggaac tagctgaatt agacattttt
    17761 tttgcgtaac gaagaatcaa tcgaaactct gcagcgtgaa caaggaatga atatcaatga
    17821 tttaaactta gcttaccatc ttcagacacc gccaaaaatc caccgagggg atctatttca
    17881 acagggaaat attcatatca gtaaacaccg tcgatatgcg gaagttcccg agcatacaca
    17941 tgattttgtt gaaatcaatt atatgtatag cggtacatgt ttacaaatta ttaatggaga
    18001 aaggatagag atggaagaaa gtgacgtggt gatcattgat aaagaagcta ctcaaagaat
    18061 cgaatacaca gggcaagagg atattttaat caacatctta ttaaaagaag aaaccttatc
    18121 tacagagata ttgaatcatt tggcaagcac aaataatcta ctttcttctt ttttgataaa
    18181 tgcatcaaaa gaaaaagggg aacatacaca attcattcat tttcgcggcg gaaaaacaag
    18241 tcgtttgacg catttgattg aaaatatgct gttgatttat tttgatacat acagtcataa
    18301 gatccgttcg ttaaatctct atacatcact attactgatc gaattgactc atttattaga
    18361 acaacaaagc ttttcatcag cagatggatg cgcagaaaat gagatgctta gagtattaga
    18421 ttatatcgat aagcatttta cttcttgtac gttaaaagaa acagcagctc attttggcta
    18481 caatccggtc tatcttagta ataaattgaa aatgtatttt gatgcttcct ttcaagaact
    18541 ggttctaaag aaaaaattcg actttgcgtt ggaactgatt caagaaacga gtttttcgtt
    18601 ggaagaaatc gctaatcgta tcggatataa acaaacgact tctttattca aattattcaa
    18661 gaagtattca acatgtactc ccaatgaatg gagaaataaa cattaagaag aaaatctttc
    18721 actaaagcat atgaaggaaa attgttttag gtgcgattgc tgtcttatta tatcgttgca
    18781 gcgaattttt ttggatttat tttcagttct aaacttttgg ttgtattatc aggggatata
    18841 ggtgtaaaca tttgtcattt atctgttatg cgtattcagg aattcgtttc catcaaaaat
    18901 tataataaca actattataa ttaacatgtt gataaaatga ataaacaaga ctatactttt
    18961 catgaaagta acaaatagtt atgaaaagga gctgtttatt catgaaaaaa acaagtcgta
    19021 aagtagtaat cgtcggtaca ggttttgttg gaacaagtat cgcttatgca atgatcaacc
    19081 aaggaatctc taatgaatta gtattgatcg atgtgaatca agaaaaagca gaaggggaag
    19141 cattggacct tttagatggt atggcttggg gcgatgaaaa tgtggctgta tggtcaggtg
    19201 gctatgaaga atgtaaagat gctgatatcg ttgttatcac agctggtatc aaccaaaaac
    19261 ctggtcaatc tcgtttagat ttagttaaaa caaatgcgtc aatcatgcgc caaatcgtaa
    19321 aagaaatcat gggatcagga ttcgacggta ttatcgttgt tgcttcaaac ccagtagata
    19381 ttttgacata tatcgcttgg aacgaatctg gtctgccaac atcacgtgtt atcggtactg
    19441 gaacaacatt ggatacaact cgtttccgta aagaaatcgc attgaaatta aaagttgacc
    19501 cacgcagcgt ccacggctat atcttaggtg aacatggtga ttcagaagtt gctgcatggt
    19561 cacatacaac tgtcggtggt aaaccagtgt ttgaaattgt agaaaaagac catcgtattg
    19621 cccaagatga attagatgtg attgctgata aagtccgtaa tgctgcttat gagatcattg
    19681 atcgtaaaaa agcaacttac tatggtattg gtatgagtac tgcacgtatc gtaaaagcta
    19741 tcttgaataa cgaacaagct gtattgcctg tatctgctta tttaactggt gaatacgacg
    19801 aaaaagatat ctttacaggt gtgccatcaa tcgttgacga aaatggtgtt cgtgaagttg
    19861 ttgaattatc aatcaacgaa gaagaaaaag caatgttcag caaatcaaca agtgctttaa
    19921 gagaagtatt gaacactgta ttataagaaa caatgaatat gaactaaaaa tcctaaaact
    19981 gcttaacagc tgaaatagct gttaagcagt tttttattga tatctaatag gtaagcaaat
    20041 aaatagaatg gttaaaatta atcggtccga aaatgtatcc gtacatatga acatatgaaa
    20101 tttaatctgt tgattacaat aagcgaatcg ttatcaatgt ttatctgtgg ttaattaaaa
    20161 aatccttccg caacggcaaa tacaatgatt catatctaat tcgttcatat ttgattaact
    20221 gaataaagaa actggttttt atcattcaat taaacgacaa aaaaagtaag aaaaactgca
    20281 aaatt
//
LOCUS       DASJST010000040        20207 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJST010000040 DASJST010000000
VERSION     DASJST010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.040"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1835)
                     /locus_tag="U4B05_001186"
     CDS             complement(<1..1835)
                     /locus_tag="U4B05_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2050338.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="U4B05_001187"
     CDS             complement(1832..2536)
                     /locus_tag="U4B05_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2050339.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="U4B05_001188"
     CDS             complement(2548..4857)
                     /locus_tag="U4B05_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2050340.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="U4B05_001189"
     CDS             complement(5052..5516)
                     /locus_tag="U4B05_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050341.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="U4B05_001190"
     CDS             complement(5516..6142)
                     /locus_tag="U4B05_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2050342.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="U4B05_001191"
     CDS             complement(6149..6514)
                     /locus_tag="U4B05_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2050343.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="U4B05_001192"
     CDS             complement(6504..6842)
                     /locus_tag="U4B05_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050344.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="U4B05_001193"
     CDS             complement(6832..7158)
                     /locus_tag="U4B05_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2050345.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="U4B05_001194"
     CDS             complement(7139..7417)
                     /locus_tag="U4B05_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2050346.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="U4B05_001195"
     CDS             complement(7419..8783)
                     /locus_tag="U4B05_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2050347.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="U4B05_001196"
     CDS             complement(8796..9365)
                     /locus_tag="U4B05_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN2050348.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="U4B05_001197"
     CDS             complement(9337..10524)
                     /locus_tag="U4B05_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2050349.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="U4B05_001198"
     CDS             complement(10588..12315)
                     /locus_tag="U4B05_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2050350.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="U4B05_001199"
     CDS             complement(12312..12764)
                     /locus_tag="U4B05_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN2050351.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="U4B05_001200"
     CDS             complement(12876..13256)
                     /locus_tag="U4B05_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2050352.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="U4B05_001201"
     CDS             complement(13253..13639)
                     /locus_tag="U4B05_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050353.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="U4B05_001202"
     CDS             complement(13641..13919)
                     /locus_tag="U4B05_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050354.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="U4B05_001203"
     CDS             13967..14695
                     /locus_tag="U4B05_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN2050355.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="U4B05_001204"
     CDS             complement(14841..15317)
                     /locus_tag="U4B05_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN2050356.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="U4B05_001205"
     CDS             complement(15277..15624)
                     /locus_tag="U4B05_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050357.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="U4B05_001206"
     CDS             complement(15611..15796)
                     /locus_tag="U4B05_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050358.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="U4B05_001207"
     CDS             complement(15797..16069)
                     /locus_tag="U4B05_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN2050359.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="U4B05_001208"
     CDS             complement(16066..16269)
                     /locus_tag="U4B05_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050360.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="U4B05_001209"
     CDS             16394..16606
                     /locus_tag="U4B05_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050361.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="U4B05_001210"
     CDS             complement(16639..16833)
                     /locus_tag="U4B05_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050362.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="U4B05_001211"
     CDS             complement(16846..17028)
                     /locus_tag="U4B05_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050363.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="U4B05_001212"
     CDS             complement(17025..17165)
                     /locus_tag="U4B05_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050364.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="U4B05_001213"
     CDS             complement(17655..18020)
                     /locus_tag="U4B05_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050365.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="U4B05_001214"
     CDS             complement(18060..18362)
                     /locus_tag="U4B05_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2050366.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="U4B05_001215"
     CDS             complement(18362..18661)
                     /locus_tag="U4B05_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050367.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="U4B05_001216"
     CDS             complement(18654..18818)
                     /locus_tag="U4B05_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050368.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="U4B05_001217"
     CDS             complement(18824..19093)
                     /locus_tag="U4B05_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050369.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="U4B05_001218"
     CDS             complement(19105..19854)
                     /locus_tag="U4B05_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050370.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..>20207)
                     /locus_tag="U4B05_001219"
     CDS             complement(19851..>20207)
                     /locus_tag="U4B05_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050371.1"
                     /translation="NVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCI
                     CGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGI
                     KLNQKTIKKLNIGGEK"
BASE COUNT         5603 a         4273 c         3141 g         7190 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacatt
//
LOCUS       DASJST010000041        20183 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJST010000041 DASJST010000000
VERSION     DASJST010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.041"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="U4B05_001220"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="U4B05_001220"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN2050372.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="U4B05_001221"
     CDS             1013..2260
                     /locus_tag="U4B05_001221"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN2050373.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="U4B05_001222"
     CDS             2336..2680
                     /locus_tag="U4B05_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050374.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="U4B05_001223"
     CDS             complement(2797..3123)
                     /locus_tag="U4B05_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050375.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="U4B05_001224"
     CDS             complement(3496..4698)
                     /locus_tag="U4B05_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2050376.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="U4B05_001225"
     CDS             4815..5330
                     /locus_tag="U4B05_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2050377.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="U4B05_001226"
     CDS             complement(5529..5975)
                     /locus_tag="U4B05_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2050378.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="U4B05_001227"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="U4B05_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2050379.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="U4B05_001228"
     CDS             complement(7854..8483)
                     /locus_tag="U4B05_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2050380.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="U4B05_001229"
     CDS             complement(8467..8931)
                     /locus_tag="U4B05_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2050381.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="U4B05_001230"
     CDS             complement(9418..10686)
                     /locus_tag="U4B05_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2050382.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="U4B05_001231"
     CDS             complement(10917..11654)
                     /locus_tag="U4B05_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2050383.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="U4B05_001232"
     CDS             complement(11721..12290)
                     /locus_tag="U4B05_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2050384.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="U4B05_001233"
     CDS             12418..13290
                     /locus_tag="U4B05_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2050385.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="U4B05_001234"
     CDS             13632..14504
                     /locus_tag="U4B05_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2050386.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /locus_tag="U4B05_001235"
     CDS             14501..16459
                     /locus_tag="U4B05_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2050387.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="U4B05_001236"
     CDS             complement(16710..17561)
                     /locus_tag="U4B05_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2050388.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="U4B05_001237"
     CDS             17684..18151
                     /locus_tag="U4B05_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN2050389.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="U4B05_001238"
     CDS             complement(18414..18896)
                     /locus_tag="U4B05_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN2050390.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="U4B05_001239"
     CDS             complement(18996..19859)
                     /locus_tag="U4B05_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2050391.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6068 a         4157 c         3662 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc ttt
//
LOCUS       DASJST010000042        20060 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJST010000042 DASJST010000000
VERSION     DASJST010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.042"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(6..866)
                     /locus_tag="U4B05_001240"
     CDS             complement(6..866)
                     /locus_tag="U4B05_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN2050392.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(925..1446)
                     /locus_tag="U4B05_001241"
     CDS             complement(925..1446)
                     /locus_tag="U4B05_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN2050393.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            1724..2485
                     /locus_tag="U4B05_001242"
     CDS             1724..2485
                     /locus_tag="U4B05_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2050394.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            2511..5102
                     /gene="mprF"
                     /locus_tag="U4B05_001243"
     CDS             2511..5102
                     /gene="mprF"
                     /locus_tag="U4B05_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2050395.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(5290..6477)
                     /gene="tuf"
                     /locus_tag="U4B05_001244"
     CDS             complement(5290..6477)
                     /gene="tuf"
                     /locus_tag="U4B05_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2050396.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            6845..7201
                     /locus_tag="U4B05_001245"
     CDS             6845..7201
                     /locus_tag="U4B05_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2050397.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(7358..7879)
                     /locus_tag="U4B05_001246"
     CDS             complement(7358..7879)
                     /locus_tag="U4B05_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2050398.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            8078..11770
                     /gene="nifJ"
                     /locus_tag="U4B05_001247"
     CDS             8078..11770
                     /gene="nifJ"
                     /locus_tag="U4B05_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN2050399.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            11959..12846
                     /locus_tag="U4B05_001248"
     CDS             11959..12846
                     /locus_tag="U4B05_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN2050400.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            12843..14012
                     /locus_tag="U4B05_001249"
     CDS             12843..14012
                     /locus_tag="U4B05_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050401.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            14036..15391
                     /locus_tag="U4B05_001250"
     CDS             14036..15391
                     /locus_tag="U4B05_001250"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN2050402.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(15463..17268)
                     /gene="glmS"
                     /locus_tag="U4B05_001251"
     CDS             complement(15463..17268)
                     /gene="glmS"
                     /locus_tag="U4B05_001251"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN2050403.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            17677..17967
                     /locus_tag="U4B05_001252"
     CDS             17677..17967
                     /locus_tag="U4B05_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050404.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            17980..19005
                     /gene="sppA"
                     /locus_tag="U4B05_001253"
     CDS             17980..19005
                     /gene="sppA"
                     /locus_tag="U4B05_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN2050405.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            19008..19667
                     /locus_tag="U4B05_001254"
     CDS             19008..19667
                     /locus_tag="U4B05_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2050406.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            19824..>20060
                     /locus_tag="U4B05_001255"
     CDS             19824..>20060
                     /locus_tag="U4B05_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050407.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEF"
BASE COUNT         6188 a         3794 c         4146 g         5932 t
ORIGIN      
        1 tatctttact tatattgttc ctccaaacgg ctcatccacc aacctagtgc tgtaccggct
       61 ataaaaagaa caggacaagc tagtacccca gcaactccca atcccgcgta atcggtagga
      121 atgatcataa tcgttgaagc aaaaactaca cctaaaatga aatggaatag cttgctatat
      181 gcctttgaga aaatgtaatc catgattttt gacaatccaa gaacacaaag aatcccacca
      241 attccgatcg gcacaatcac ttcaagtcgt acttctttaa atccgtcagc catcgcttta
      301 tacaatccca tatacaccaa aaaattagaa gggctcaatc ctgggacgat catacccaaa
      361 ccaatcaatc cgccggcaat cagccatgtt ccaaaattct gatcgacttg cccgtcaaat
      421 aattgagcgc cgtataataa aaacaaataa gccagaacaa aagtaacaac catgatgatc
      481 acatcagaag tatctcttcc ttttttccct gcttctttcc ataacgcagg aacagttcca
      541 gcgatacatc cgacaaaaaa ccataatatc gttgtttcat acgcacctaa taaaaaactg
      601 actaaaaaag aaagtagaaa gatgcctgtc actccgccta gaccaacagg cagaaaaaac
      661 aaaacatttt ctttgaaatt ttttgtaagg tgggctaaaa aagaaatcag ccgctcatac
      721 atgccaaaga cagctgctaa tgcaccaccg ctgactcctg gcaagataaa tcctgaaccg
      781 ataaacattc ccttcacaaa ccgtaacatc cagtctttgg ttgtgtaccc cacttcttga
      841 ttttccattt tttgcctttt ttccactatt tgcgtactcc ttctttcatt taattttatt
      901 catgattgaa catttcctgc tattttactt aaagactttt tcataggtct ctattatttt
      961 ttcattcatt ttgacaatat caggataaaa catgttacct ccattatgat agagatagcc
     1021 gccattgtaa agcaaggact ggatccgcca atatcggtat tgtttccctt cctcatttcc
     1081 taaaagtggg ctcagcacat ctttagaata cgcttctgct aattctactg tgttttctcc
     1141 gccatgattt tttacatagg caatataatc tttcccaaaa ttatatgctt ggacagctgt
     1201 ccataaatcg catccttgtt tctttgcttc tactatcatt tccgccaaat atttgacacc
     1261 ttgatcaata ctgtcagctg gattatcaat gatctcagct tctccatgca gactttccga
     1321 gctctgcatg agatctgtcc cttttccttt tgattcggtc aatattatcg ccttgatcaa
     1381 attctcgtag ttttccatat gataattttt tgcagctgtt gttatttcta tttgatagct
     1441 ttccactcgt tttaccgaac gataagttct tcctcataaa aacaacaccg caccaataac
     1501 cgtcagaatg atcatgtaaa aaagaatacg gataatagat aatcgttttc gcttcatttc
     1561 atcacctagt tattttgaca tcttttttta tcttactatt tctttatctt cttggctatt
     1621 tcttccttga tttctgtgta cgccttaaga aaaaatgaat tttcctgtat tttttgacat
     1681 cttacgaaaa tgatcgttgt ttttcagtaa aaacaattgc ccaatgactg atataatcgt
     1741 tatactttac acagtaggtg attatgtgaa aaaagaaaag aaatatgcaa aaatgccaga
     1801 tgggagcgaa atttactatg aaaagagcgg tcaaggcttc cctctctttt tactgcatgg
     1861 caatgacggc agcggccgct ttttttcaga acaggtccct gtactcgaac gctactatac
     1921 cgtgtatcta gtagatagcc gaggacatgg tcgttcgaca aacgaagcat ctatgttgaa
     1981 ttttcagtta atggccgagg atctgaatac gatcatgttg ctggaaaaaa tcgatcaggc
     2041 agattttctt ggatttagtg atggagcaaa tctagcactg gtatttgcca gcagcttccc
     2101 taaaaaagtt catcgattga ttttaaattc tggaaacacg cttgttaaag gcgttcgttt
     2161 ttctgccaga gtcatcagta atattcatta tgcatgggta tggttactct ctttatttcg
     2221 cccaagtctt cgcaaaaatc ttttagttat caaacttttg ctgcatgaca tcgggcttac
     2281 agaaaatgat ttaaaaaaaa tcaactcccc cactttgatc atcgtgggaa aaaaagatgt
     2341 aatcaagcta aaacattctt tgtatatagc taaaactata ccaaaagctt cctttgtgct
     2401 tgtgaaagaa caaggacatg aattagctag aaaagatcct gaacgcttca atcgagaagt
     2461 tcttcaattt ctttctgaaa cataactatt aggaatagaa ggtgatttat ttgttaaaaa
     2521 ataccataca atggctgaaa aaccacctag gactgttcaa aacaatcttt ttgatttctg
     2581 tcattgtcat tattgtcggt gaattgatgt ccatcggtaa aactctatct atccagcaat
     2641 taggagaaac gtttgccact atacctgtat ggaaaagcgg actaatgcta ttgattggtc
     2701 ttatctctgt gttacctatg gttggttatg atattatttt gaatcggatg cttgaacaaa
     2761 agcagaatcc tcgttacctg tttgagacaa gctggctgat caatacgatc aacaatattg
     2821 ccggtttcgg cgggtttgtt agtgtggggc ttcgctctga actatatgga caaaagaaag
     2881 agagcagaaa cgtgatccaa gcattatcga aagttttctt gtttttgatg gctggacttt
     2941 ctatttacag tctgctttct tttttacttg tagtcttcac gccagttgct ccttttttga
     3001 agcaatattg gatatggttg atcggcggcg gtctatattt tcctgtcgtc tttttattta
     3061 ctactttcaa aaagaaagga ttccttggtg gtacctcact taaaactcaa ggacagctat
     3121 tgctagtttc attgttggaa tggaccggtg tcctgtttag tttcctttct gttggttatt
     3181 taatggaaat ccggattgat ctgtggcaaa caattccatt attcattgct gcttcagtga
     3241 taggaatcgt gtctatgatt ccaggagaaa ttgggagttt cgatgtaatg atgattatcg
     3301 ggttgtccgc tattggtgtg ccaagagaaa tagtcgttgt gtggatcttg ctctatcgcc
     3361 tgttctacta catcattccg tttttgattg gaatcgtctt tttcttcaaa aatatcggtt
     3421 ccacttttga tcagcgttat tctggtatcc ctaaacaatt agcaacagaa attgcgcata
     3481 aaatcgtggt tgtcttatta tacttttctg gaatcatgct agtactttct gccactatcc
     3541 cgcaagcttt tacagagttt cgctggcttc atagtttgaa cccattgaaa tttcatttta
     3601 ttatccaatt tcctagtata ctattgggat ttcttttaat cattatgggc agagggatcg
     3661 cagcacgtgt gaaacgagca tatttgccta caatcttctt gattgttcta gctttacttt
     3721 atgttttatt aagtgatttt agtttcacac cggtggtttt cttgtctatt ctgcttctga
     3781 tcattcttgc ttctaaaaac gaattatttc gggaacaatt gatctattca tgggagtggc
     3841 gtacgatcga tggaatcatt attggtgcac tgtccttgct ttacatcgtt attggggtat
     3901 acaatctgcc ggatttccct catcggcgtc accattttat ttcctttttc ttatttcctt
     3961 ctgaaaagat ttggttttcg ggattgttgg ccatcatcgc tgttagtttc atgatcgttc
     4021 tatttgttca tttccttcaa ggagaaaaga aacagattgg cgaagccttt aatgaagaaa
     4081 aggccctgaa gatccttaca acttatggag gaaactccga tagtcaattg atcttcttaa
     4141 aagataagcg gatgtttgcc tatgaaaaag acggtgaacc aactgttctt ctccaattcg
     4201 cttgcttcaa taataaatgt attgtaatgg gtgatccttc tggtaagaaa gaagattttc
     4261 cagaagcaat cgaagctttt atcgaagaaa cagatcgact atgctattta ccagttttct
     4321 acgaaacaag cgaagagatt gttatgattt tgcatgaatt cggctacgat ttcattaaga
     4381 tgggggaaga agcttatgta gacctgaata gttttacaac ttcagggaaa aaaatgaagg
     4441 gcactcgtgc tgtcttgaat cggatcgaac gagaaggatt tacatttgat gtcttacaac
     4501 caccattttc tactgagcaa atgagtactt tcaaaaatat ctctgataat tggttgggtt
     4561 cacgaaagga aaaagggttt tctttaggct ttttctctga agattatcta caaagagcgc
     4621 ctattgcagt tgtcaaaaat acccaagaag aaattgtcgc ttttgcaacg atcatgccta
     4681 cttacacgaa caataaagta ggaacgattg atctgatgcg atatgattct aaaaaagcac
     4741 cttctggcag tatggatttc ttgttcttgc atttgtttgc ctatatgaaa gaagaacata
     4801 tccaatggtt caatttaggg atggcccctc tttcaaatgt cggaacttcc agaaagagtt
     4861 ttttacagga aaggattgca tacttggttt acgagtttgg ttctcatttt tattctttcc
     4921 atggattaag agaatataaa agcaagtacg ctacaaactg ggtctcacgt tatactctat
     4981 attctagaga tagttggatt gcatatgtta tgattgcttt gctgattatt gataatgcgc
     5041 cagttgagca gacacaaggt agaataagtg gtttgaaaaa atggatacga agaaagtatt
     5101 aaaaactgca taccgataga tgaacactct catgatgaat aaaagagact gaagtaaaac
     5161 gaaagttcat gcttctacaa tctcttctta tacaattgaa gaacaaagag cggtccaaac
     5221 agaaatcctg tttggaccgc tcttttattt taaacatttt tcattaccaa aatattcgag
     5281 tcgcgtttct tatgcttcga tctctgtaac gatacctgaa cctactgtcc gtccgccttc
     5341 acgaattgag aatgtcgttc cgttttcaac agcgattgga tggatcaagt caacatcaat
     5401 cgtcacgttg tcaccaggca tgaccatttc tgtatcttct ggcaatgtga ttgttcctgt
     5461 aacatcagtt gtgcggaagt agaactgtgg acgatagttg ttgaagaatg gagtatgacg
     5521 tccaccttct tcttttgtca acacgtacac ttctgctttg aattttgtat gtggagtaat
     5581 ggaacctggt ttagcaagta cttgtccacg ttcgatatcg tcacgttgga tcccccgtaa
     5641 caacacgcct acgttatccc cagcttcccc gtaatctaac gttttacgga acatttctac
     5701 acctgttaca actgcttttt gtgtttcagg tttgatccct acgatctcta cctcatcacc
     5761 gacacgaaca gcaccacgat caatacgacc tgaggcaaca gttcctcgac ctgtaattga
     5821 gaagacgtct tccactggca atagtaatgg tttatctgta tcacgttctg gtgttgggat
     5881 atattcatct actgtatcca tcaattccat aatagcagct tcagcatctg gatcgccttg
     5941 caatgctttt aatgcggaac ctttgatcac aggagtgtca tcgcctggga aaccatattc
     6001 gctcaataac tcgcgaactt ccatttctac caaatcgatc aattcttcat catcgactaa
     6061 atcaactttg ttcaagaaaa caatcaggta ttttacacca acttggcgtg acaataaaat
     6121 gtgttcacgt gtttgaggca tcggaccgtc agttgcagaa acaactaaga ttgcaccatc
     6181 catttgtgct gcccctgtaa tcatgttttt cacatagtcc gcgtgtcctg gtgcatcaat
     6241 atgcgcgtag tggcgttttt ctgtttcata ttctacgtgt gcggtattga tcgtaatccc
     6301 acgttcacgt tcttctggag ctgcatcaat actagcatag tcttgaggat ttgctaggcc
     6361 ttttttgcct aatactgtag tgatcgcagc agttaaagta gttttcccat ggtcaacgtg
     6421 tccaatcgta ccaatgttaa cgtgtggttt acttctgtca taatgttctt ttgccataat
     6481 ttacaaacct cctaaataat atagatcact gaaaaatttc agctctcacc tttttattat
     6541 agtctttttt tgactaaatc aaaaagaata cgtccgactt actttttgta agattcgaac
     6601 taagcttatt atacgtcaat attggtcaaa gtcaactaag agcactcgtc tgtcttatac
     6661 gcgaaaaaag taccttttat aaataaaaaa atgtgttttt tgagaaaaac ttcaagatag
     6721 tccttaactt tcttttgaag caataagaac aaaatgagga aaagtttaaa attcagctgt
     6781 tgccaacaac tttcaataga cgaaatcttc tatttcgtta ataataagga aagaggtgaa
     6841 gtacatgaaa aaattaattg gcgtacttgt cgttttaggc atatttctcg gcggaagttt
     6901 tgcagcttat cattattttt acggcggcga agcttattat acaaaaatca cgacgaatgg
     6961 cgaaatgtca gctacccaag cagataacgg agagaaattc acgacttata cctacaagca
     7021 agcggcttat aataaagatg gtgaaaaaaa acaggtgacg cttcgcgaag aaagagaaca
     7081 tccattaaag atgaatgcgt atcttaaact caaagtcaat cctcgtaaag gtgtattaag
     7141 ctgggaagaa ataaaggctt ctgaagtgcc taaaaatgcg gcagaaaaaa tcgataatta
     7201 gtaaatggtc ttaaaatttt attagataaa aagaggctgg gacagaagcg tttaactcca
     7261 agaaataaga aggagttgct tctgcttccg ccgtttatac gtgtttagag cgtgagacaa
     7321 aagtgttctt tacttttgtc ccacgctcct tattttttta ttttgtttgg atcaattctt
     7381 ttaattcctc tattgaaata gattcatcaa agatttcttt acccaagatg acagttggaa
     7441 caaatcgaat atgtgcttgc tcagcttcgt tgatgacagc ttggctcagc tgttcgtctt
     7501 tttgttctgt cagctttagt ttatctactg caaaatccgc tacttcttgg agagaaagat
     7561 gtttccactc gtcttgagta tcgaatattt gtttgatctc tttgatcgct ttttggccat
     7621 cgctgattgt gagataccgg tgcatcacgt tccctcgctg taagctttct ttttctttat
     7681 cgaacaattt gatcactcgc tgcaattgcc cgctttggac tgcttcttct aatagctcat
     7741 aagattcttc aaaccattgt cggcaatacg gacaagctaa gttgatgaat tcgaccattt
     7801 ttttaggagc attcgatgaa ccataaagga ttcctttttg tgtatttgtt ttcgttgcat
     7861 caatcactga tatatccatg ttaaaacacc tctttcgctt atagtttata catgtctgac
     7921 aaaaaaaggc aacgatttag actgtttgtc taaaaattag atgaccaatt caaatagata
     7981 catgtaaaat atttttataa ataaaagaaa aaaatggcat gttttacgtt ataatggaac
     8041 agataataag agagaatatg aacaaggatg atgaatcatg cgaaaaatga aaacaatgga
     8101 tggcaatacg gcagccgcat atatttctta tgcatttacc gagattgctg caatatatcc
     8161 aataacaccc agttcgacca tggctgaatt agtagatcaa tggtcagcag caggaaaaga
     8221 aaacgtattc ggtcaaccag taaaagtagt agaaatgcaa tccgaagcag gagcagctgg
     8281 tgtggtccat ggatcattga aaactggtgc actgacaact acatataccg cttcacaagg
     8341 gctgcttttg atgataccga atatgtacaa gattgctggg gaactactgc caagtgtttt
     8401 ccacgttgct agtcgggcag tgacgacaaa tgcgttgaat attttcggag atcacggaga
     8461 cgtgatggct gcaagacaaa caggatttgc tatgttagct gaaagcagtg tacaagaagt
     8521 gatggattta gcacctgttg cccatcttgc ttctattgaa ggagaagtcc cttttttgaa
     8581 tttctttgat ggattccgta cgagtcacga gattcaaaaa atcgaagttt tggaatatga
     8641 agaattagct cctctgatca atcaggagaa attagcttca tttcgtaaac gagggatgaa
     8701 tcccaatcat ccgactgtta gtggtacgaa ccagaatcca gacatccatt ttcaacaacg
     8761 ggaaacgatc aacccctatt atgataaatt acctgaaatc gttcaaaaat atatgaaaga
     8821 gatcaaccga ttacgaggaa cttcttatga cttggtgact tattacggag cagacgatgc
     8881 ggaagaagtc attgtttcta tgggatcagt tgcacagacg attgaacaaa cgattgatta
     8941 cttgaatgca aacggacgga aaacaggttt tttgaatatc catctttatc gtccttttcc
     9001 agtggaaaca ttcgtgcaaa agttgcctca tacagtaaaa acaattgctg ttcttgatcg
     9061 aagtaaagaa ccaggggcag gtggagaacc tctcttatta gacgttcaaa gtgcgttata
     9121 tgattctgaa cttcgcccgg ctgttattgg tggtcgctac ggattggggt ctaaagatgt
     9181 gactcccgac caaatctcag cagtttttga tgaattgaaa aaagacccaa gcatacgcaa
     9241 aaaacggttt accataggga tcgtggatga tgtgacttat caatctttgg aaccaaagga
     9301 aagtctggat ttgacagaac cgcaaacatt ccaggccaag ttttggggat ttggatctga
     9361 cggaacagtt ggagcaaata aatcagctat caagattatt ggagatcaca ccgataaata
     9421 tgcgcaaggc tatttttatt atgattcgaa aaaatctggc ggtctgactg tctctcatct
     9481 tcgttttgga gatacgccga tacgttcagc gtatcttgtc gagcatgcgg accttgttgc
     9541 ctgtcacaca ccagcttacc tgcatagcta tgacttggtc aaaggattga agccaggtgg
     9601 cattttttta ttgaatactc tttggagcga cgaacaatta gaaacgcatc tgcccttaaa
     9661 attaaaacgt tacttggctg aaaataacat tagattctat acgatcaatg ccatgagatt
     9721 ggcccaagaa gtcggtttgg gacgccggat caatacggca atggaaacgg ctttttttaa
     9781 attagctgac atcattcctt ttgatgaggt gcttccgctg ctaaaagaag aagccctgaa
     9841 aagctatgga cataaatcaa tggctatcgt agaaaaaaac atacaagcaa tcgatcggac
     9901 gaccgaactg cttcatcaag tcgcgatccc agaccattgg agaacgttgg agatgcctga
     9961 aaaaatcaat aaaaggaaca ctggttatat acaagaaata cttgaaccag tgaatgcaca
    10021 ggaaggaaat caactctctg ttggtacact tgtagcaaat ggaatgacaa ctggcgaaat
    10081 gcctttagga acggcttcta tggaaaagcg gggcgtcgct ttagaagtac ctgagtggat
    10141 tagtgatcgc tgtacgatgt gtaatgaatg tgcctttgtt tgtcctcatg ctgccatccg
    10201 tccattttta gcagatgaag aggaaatgga agaagcgcca gaaggattta tcgtgagaga
    10261 aatgcgaggc gcagacggcg taaaataccg tatccaagtt tcagtggaag actgtacagg
    10321 ttgtggtctg tgtgtagagg cttgtcccgc aaaagaaaaa gctttagtga tgaagcctta
    10381 tgaagaacag aaagaacaag ctgtgaattg ggcttttgca atgacattaa gacaaaaagc
    10441 aaatccagtc aaaggaaaga atactgtctt gtctacacaa ttcaatcaac cgcttcttga
    10501 attctcgggc gcttgctcag gctgtggcga aacaccttat gtaaaattat tgacacaaat
    10561 gttcggtgat cgaatgatga tcgcgaatgc gacaggctgt tcttctattt ggggcggtgc
    10621 ttctcctgct tctccttata cgaccaatga atgtggtcaa gggcctgcat ggtcaaattc
    10681 tttattggaa gataatgctg aatttggtta tggtatgctt ctagcggcac agactcgtcg
    10741 cgaggctttg gcaagttcaa tcaaagaagt attgccaaaa gtatcagaag aactacgaga
    10801 attgatggaa gactggttag tacacatgtt tgagagtgaa ggaacgcagc aaagagcagc
    10861 aaaactgcaa gctgcgatcg aaagcgaacg atcaattttc cctgaactgg aggatatata
    10921 caagagaaag gatctattcg taaaaaacag ccaatggatg attggtggag acggatgggc
    10981 ttacgacatt ggttttggcg ggatcgatca tgtacttgcc agcggagcag acgtgaatat
    11041 ccttgttttg gataacgaag tctattccaa tactggcggt caaacctcaa aagcaacacc
    11101 agcttcggca atcgctaaat tttcggctag tggaaagtat gcttcaaaga aagatctagg
    11161 aatgatggcg atgacatatg gaaatgttta tgtggcgcaa attgcttcag gtgccaatca
    11221 aatgcaaacg atcaaggcat ttgaagaagc agaaaagtat cctggtcctt ctctgattat
    11281 tgcctatacg ccttgtatca cacatggctt gcttggcgga atgaaagaat cgcttaaaga
    11341 agcaaaagaa gctgtttctt ctggttattg gtcattatat cgatataacc ctacaatggt
    11401 cgaaaaaaac aagaacccaa tgactttgga ctacaaaaaa cctgatttct ctgctatgcc
    11461 tgatttcatg aggaaacaag ttcgcttttc ctcgctagat aacgcaaatc caaatcttgc
    11521 aaaagcttta tttgaaaaga ctgtccacga cgctaaaaca cgtttttaca actatgctgc
    11581 actgacggga gaattagaaa aaatcaagaa acgacttgaa ccagaggaat ctatcgatgt
    11641 acccgttaag actagaaaag aaaaaacgac ggaccttgaa gcagaagaaa gaagagctgc
    11701 tagaagagct gccagagcag aacgacgtaa aaaaatggaa caagctacaa aagaaaaaaa
    11761 ggaaaaataa cagaaaaaat ataatgaata attctctgaa gtttaaaaat atttattgca
    11821 gagaaaaata aaacgcatac ttgcaatttt cttgaaaatt atgcgttttt ctcaagaaaa
    11881 atacgagcat atcagcctct aaaattaaaa catatgatat actagagagg aaatctcaaa
    11941 agagaagaga ggctttttat gtcatttcaa atcggggaac tttttaattt ggactattgg
    12001 cagcaaatgt tttccggtga cttgttctct ctgaattttt tggtgaacat acttgatatt
    12061 ctggttgtct ggtatcttgt atacaaactg atccaactgc tgcgaggcac aaaagcgatt
    12121 cagcttctga aaggtgtagc tatttttatt gttatccgaa tcgtagcgga aatcattggt
    12181 ctgcacacct tgtcttggct gatgaaccaa gtcattacgt acggggtgat agctgctatc
    12241 gtgatttttc agccggaaat ccggagagga ctggaacatc ttggacgcag ttcgcttttc
    12301 aaacaatcaa aaagcgaaca gcaggaagat gagaagatga tcttgtcttt tgacaaagcc
    12361 atccaatata tgtccaagcg aaaaatcggt gcgttgatca ctatcgaacg tcatactgga
    12421 ttagatgagt atattgagac agggattgct ttagatgcag atatcactgg ggagctgctg
    12481 atcaatatct ttattcctaa tacaccgctg catgacggtg cagttattgt aaaagaagga
    12541 aagatcgcag tagcaagcgc ctatcttcct ctatcagaaa gtatgttgat ccctaaagaa
    12601 ttcgggactc gacaccgggc agctgtcggt ataagcgaag tcagtgacgc gattaccatc
    12661 gttgtctctg aagaaaccgg ggacgtcagc atcacgttag ataatgaact gatggctggc
    12721 ttatcccagc aagaatattt ggcaatcttg cgtagagaac taattgctga agagaaaaca
    12781 aaaaacaaga aaaacgtgat tcaatctttc ctagacggta tgactaaggg aggggatcgc
    12841 aaatgatctc aaaagagaaa cggacaagca ttctttacag ttttattgca ttgctgttca
    12901 gtattgcgct gtacttcaat gcaaatggcc agtccgttcc aaatacgctt tctggaaatg
    12961 aagcgtataa tcaaacagtt acgggggtgc cgatcaagat ttcttatgat tcaaagcgct
    13021 actatatcca tggatatgaa aatgcagtga ctgtcaaact ttccagtgca aaccgagttc
    13081 agttgaatgc agaggcaaac gaagatacac ggatgttccg cgtcacagca gatatcacta
    13141 agctgggcga aggaacacat gaagttccgc taaaaattca aaatttgagc agtgctgtca
    13201 cagcaaagat cgaaccatcg actattacag taacggttga gaaaaaggta ataaaagatt
    13261 tcgatgtgga aacgttgatt ccttcaacgt tcacaccatc tggatatgaa cttgatgata
    13321 cttccgttag tcctaaaaaa gtctctatca ctacaggtga caaaacattg gcagaaatca
    13381 aacgagtagt agctgatgtg gacgcttcga tggtcacaga tgatgggatt aatagtgagg
    13441 tcccaataca ggcgctgaat gcagcagggg aagtattaag tatcgtctct gatccagtgc
    13501 aagtaacagt gagagctgat tcgatcaagc caagcaagtc ggtacgactt tatgggatac
    13561 agcaaggaac accagctgcc ggggtagaaa gctatgattt cagtttttca aaactggaag
    13621 cagaagtaag cggctctagc gatttgcttg catcgatcgg tgacagtatt gctgtaccga
    13681 tcaatgtctc aggtatcaat catcgaacaa ctcgtaaaat tgaaatacca gtagaagagg
    13741 ggttaagtat caagcccgaa tcagttagtg tggagattac acccgtgttg caaacgtcaa
    13801 gcagtagctc tgcttcgacg agcagtcgga cacaagaaaa tacaagtacg agtagaagta
    13861 tttcatctgc tgattcagga acggaaccat cgacaacaac catttcttcc agttcggaat
    13921 ctgggacaag cagctcgacc acaagtacag aagaaacatc agaaacaagc gaatcaacac
    13981 aaatgagcag aacaaattcc tcagaaaatt aagttaaagg agtaatttta caactatggg
    14041 taaatatttt ggaacagacg gcgtacgcgg tgaagccaac aaggaactta cgccagaact
    14101 tgcatttaag ctgggacggt gcggaggtta tgtcctaagc cagcatgaag aagaaggaaa
    14161 acgcccacgc gtgttagtag ggcgggatac aagaatctca ggtcaaatgt tagaagaagc
    14221 gctgatttca ggacttttat cggtgggtat cgaagtgttc cagctaggtg tgatctctac
    14281 accaggtgtt gcttatctga cacgtttgca aaaagcaagc gcaggggtca tgatttctgc
    14341 ttctcataat ccagcacaag ataatggaat caaatttttc ggtgcagatg gttttaaact
    14401 agtagatgac caagagctag aaattgaagc attattagat gctgaagtgg atgaattgcc
    14461 tcgtccatca gctgaaggat tgggaactgt agaagaattt ccagaaggct tattgaaata
    14521 ttctcaattc cttgtacaaa cgatcaacgg tgacctttcc ggtttgacca tttgtgtcga
    14581 tgcagcaaac ggagcaactt caacaagtgt taatcgtttg tttgccgatc ttgaaacaga
    14641 tttttataca atgggcacaa atccagacgg gttaaatatc aatgacggcg taggctctac
    14701 ccatccagaa cgtttagctg agatggtcgt tgaaaaagga gcagatgctg gactagcttt
    14761 cgatggcgat ggcgatcgta ttattgcggt ggatgaactt ggaaacattg tcgacggtga
    14821 taaaatcatg tttatctgtg ctaaatactt ggcagaaaaa aatcgattga aaaaagatac
    14881 gatcgttacg actgtcatga gtaacttagg attccacaaa gcggtggaag gaatcggttt
    14941 gaaagatgtc gttacacaag ttggtgatcg ttacgttgta gaagaaatgc gcaaaaacga
    15001 ttataacttt ggcggcgagc agtctgggca catgattttc ttagatttta acacaactgg
    15061 agatgggatg ctttcaggaa ttcagttact gaatatcatg aaacagacag gtaagaaatt
    15121 atcagagctt gcagcagagg taacaatcta tccgcaaaaa ttagtgaata tccgcgtaag
    15181 tgataaaaac ggtgcgatgg atgttccggc aatcaaaaaa gtgattgaag aggcagaagc
    15241 tgaaatggga tcagaaggaa gaatcctagt tcgtccttcc ggtacagaac cattacttcg
    15301 agtgatggca gaagcgccta ctgatgagaa agttaattac tatgtcgata aaatcgcggc
    15361 agtagtcaaa gaagaaatcg gactggatta agagatattt tcataaaaaa taaaagaaca
    15421 aggaccagtg tcagatgtct gacactggtc cttgttcttt tattattcta ctgtcactga
    15481 tttcgctaaa ttacgtggtt tgtcaacgtc gaatccccgt aatagcgttg cgtaatatgc
    15541 aagtaattga gtcggtacga ctgtggttag agtagttaga taagggtgga cagttggtag
    15601 caccagctgg tcactttcct tttctaatcc ctctgagaca ataactagtg ttttagcgcc
    15661 acggctttcg acttctttca ggtttcctcg agtatgcgca ccagtcactt cttcactgat
    15721 gattccgatc actggtgttc cttcttcgat caaagcaatc gttccatgtt tcaattcgcc
    15781 agcggcaaaa ccttctgcct ggatataact gatttctttc aatttcaaag cagcttcaag
    15841 agaaacgttg tagtcagcac cacgtccaat gtaaaaggca tttcttgtat cgctgagata
    15901 tacttctgcc aactcttcta aataatcttt ttgggcgatg accgattcca tcgcattagc
    15961 aactaaactc aattcatgag tgatatcgaa tacaagagaa gtttcaaatt cttttttatc
    16021 accgatagct ttggctaata aagttagtac agctatttgt gcagtataag ctttcgttga
    16081 tgctactgcg atttcgggtc cagcatgcaa tagcagtgta aaggatgctt cccgcgaaag
    16141 agtagaacca gcaacatttg taatcgtaag tgaagggtag ccgagacggt tgatttttac
    16201 cagcacttgg cggctgtctg ctgtttcgcc actttgactc aagaaaataa agaatggttt
    16261 ttttgaaaga agaggtgtgt tatagccaaa ttcactggct aaatgaacct caacaggaat
    16321 ctgtgtcaat ttttccaaag tctgtttacc tgctaacccc gcatggtaac tagttccgca
    16381 agcaacgata tagattcgat cgctatcaga caatgtatcg accagctttt gatcaagaga
    16441 gacacgattg tcttctgtta cgtatttttg agcgatacga cgcatgataa taggctgttc
    16501 gtcgatttct ttcagcatat aaaaaggata ggtacctttc tcgatatctg aagcatctac
    16561 ttgtgcttcg aatgaagaac gttctacgat gtcacctgca aaattttgaa taataacttt
    16621 ttctgatgtg acggtaacca tctcttcatc ttcaatctca acgaattcct tcgtatgagc
    16681 aagcatagcc attgcatcgc tggcaatcac attgaatcca tcgccgagtc cgattaatag
    16741 tggactttta tttttagcga cgtaaataac atcaggtgct gtccgatcaa tcaaagcgaa
    16801 agcgtaagaa cctttgatga cccgcaaagc ttttaaaaac gcctcttgcg ttgtagtatc
    16861 cttcgcgaaa gtttcaatta gatgagcgat aatctcagta tcggtctcac caataaagtg
    16921 gtcatttgct aaataagcaa ttttaagttc ttcaaagttt tcgattacgc cgttgtgaac
    16981 gagcacgaag cgtccacttt gggaagtatg tggatgggcg ttttcttcag aaggttgccc
    17041 gtgagttgcc catcgtgtat gaccaatacc tatatttcct gtagtatcaa tatttatttt
    17101 gtttttcaaa ttttgaattc tgccttgtgc tttgaccaga taatcagttg tgccatctga
    17161 gacaaagata ccagctgaat cataaccacg gtattctaat ttttctagac cgtcaatcaa
    17221 tcctggtgtt acatttttta atccaatcat tccaacaatt ccgcacataa aaaacatcct
    17281 actttctata atgaattcgt atgaatgctt ccagctgcag gaaaatcctt ttgttttatc
    17341 tttgcagaca ttttttgtgg attttctgta gagtgcagtc cctagggcca tccgccgaat
    17401 ctttcgataa accctctcct cgtcatctgt ttacacagac ctggcgctat cctaaaaggt
    17461 ataactatcc tcctttttgt tttttatctg gaaacattta aaatcataac cttttgcgaa
    17521 tgttcttgtc aacaaaaaaa gaataaaaaa taaattggta tatatcaaat tctttgtcaa
    17581 atgtcctttt tatgtcaaaa aataatgggt ttttatctat catctttttc aggagttcgg
    17641 tacaatagaa agtaatcgga tagaggagga gtaaaaatgg attcaaaaat tgagatcatg
    17701 acactgggaa tgttaaaaaa acaattgtcc aaattcgaag catcagctgg tgtatcagat
    17761 gatacgaaaa tatttttaga tactggctgg gatagcatcc aagaaatcgc acctgatgcc
    17821 ttggaagttg ctcaagctcg tgagtttacg gtagaagatg aattaacaaa agagtcgttt
    17881 tccgggtatg cgcgggaaga aaaagcagag cggttcgaca cttctgagca gtcagaaact
    17941 gtgatcgtca taaaaaactt gtattagaaa ggagaaagaa tgaataaaag acgttgggca
    18001 gcagttctga ttgctgcagg attatttgtg gtttcgttag tttcagctag tttgactaag
    18061 cctcaggaaa acgatcaaat tgagggattg aacagttggt tatatgggga tgaagaacta
    18121 gctccaaatg tgttggaaga gggagacagt acgcaaaaaa tcgtaaaatt atcagtagat
    18181 ggaacgattg ctgcgggagg gtcttcaggt ttatttgctt ctgaaggtta caatcataaa
    18241 aattttatgg aacaactgaa ggcgattgaa gaagatccaa cagtcaaagg tatcttttta
    18301 gaagtaaatt ctcctggtgg cggtgtctac gaaagtgcgg aaatcgctaa gaaattggat
    18361 acgattcgta aagaacatga tatcccaatg tatgtcagca tgaaaaacat ggcagctagc
    18421 ggtggctact atatttctgc tcaagcagat aaaatctttg caacagaaga aacagtgacc
    18481 ggctcaattg gtgtgatcat gtcaggattg aattattcag gacttctaga aaaactgggt
    18541 gtggaagata caacagttaa aagcggagcg ttgaaagata tgggttcagg aacgcgacca
    18601 gagacaaaag aagaagaagc ggtgcttcaa gcatatatcg acagcgcata tagtcgtttt
    18661 gtagcaatcg tcagcgaggg acggaataaa tctgaagaag aagtcaaaaa aattgctgat
    18721 ggaaggattt atgatggtgt ccaagcaaaa gaagtaggtt tagtcgatga attgggattt
    18781 cctgaagatg ctttaaaagc tatacgaaag gaacaacagc ttgaagatgc ggaactagta
    18841 gaatattctt ccacgccatc tgggtttgct agtacatggt taggaagtaa actagcagaa
    18901 ttccagggat tgaagccttc tgagactagt cagatcttat ccgttcttga gaatctaggg
    18961 acagcggaat caccaaaagc aatgtattat tacggaggtg agtaagcatg tcagagcttt
    19021 caaatgaaaa taaggaacaa gaaaaagata agcaacagca gcctgctgta ggacctgact
    19081 ccaacttttc ccaaaaggta ctgagctctt ttgaaagaga gcctttgacg cccgaagaaa
    19141 tcaaaaagaa acaacgtaaa tggaaaaagt atacggacac aagaaatgaa ccgccaaaag
    19201 caaatgattt tcctaagtat ttctatgcag gtttttttat ccgaatgttt gccttcttgg
    19261 tcgatctttt gtgtatcgga gctattacgc gtttaacact tggtattgcc aaaaatctag
    19321 gttggcttaa tttttcagat tcttatctga gtctctacgg attcacagcc ttacttattt
    19381 atctagcgta ttttatttta ttgacgaaat tcaatcatgg ccaaacgatt gggaaaatga
    19441 tctttggtat tcgagtcatc agctttgatg aagaagaact tagctggtca accgttttag
    19501 ttagagaagg tgcttgccgg ttcattctaa aatttccttt gctgatgtta gggtatcttc
    19561 cagcagtctt taatcagaaa aagcaacatc tgggcgattt tttcactaat acaagtgttg
    19621 ttacgatcaa tctgatcaaa gcgttcaacc aggaagtcaa cagctgatga gcaataaaaa
    19681 gcagtaacag ttgaaagcac cttctaaaag tcagatctaa aatctagctt ttagagggtg
    19741 cttcagttat ttttaattgt catgcttttt ttgttttgct actattgaac atgagctgaa
    19801 tatgagcaag aggaggacac tgcgtgaagc cgattgattt tccagaaaaa tatgagaatt
    19861 taatgcgagt ggcacaacaa gcactagcaa accagcaata tcagcaggca aaagaattat
    19921 ttcaacgagc ttacgagttg aaagaaagct ttgaggctaa tagcctgctt gttttttgtc
    19981 tttacgagtt ggacgagaaa aaagaagcat tgaagcaagc actcttgcat gaaaaacagt
    20041 atcttcaaaa tgaggagttt
//
LOCUS       DASJST010000043        19749 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJST010000043 DASJST010000000
VERSION     DASJST010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.043"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..181)
                     /locus_tag="U4B05_001256"
     CDS             complement(29..181)
                     /locus_tag="U4B05_001256"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2050408.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(372..1250)
                     /locus_tag="U4B05_001257"
     CDS             complement(372..1250)
                     /locus_tag="U4B05_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2050409.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1289..2407)
                     /locus_tag="U4B05_001258"
     CDS             complement(1289..2407)
                     /locus_tag="U4B05_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN2050410.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2404..4503)
                     /locus_tag="U4B05_001259"
     CDS             complement(2404..4503)
                     /locus_tag="U4B05_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN2050411.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4554..5375)
                     /locus_tag="U4B05_001260"
     CDS             complement(4554..5375)
                     /locus_tag="U4B05_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2050412.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5372..6181)
                     /locus_tag="U4B05_001261"
     CDS             complement(5372..6181)
                     /locus_tag="U4B05_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2050413.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6202..6696)
                     /locus_tag="U4B05_001262"
     CDS             complement(6202..6696)
                     /locus_tag="U4B05_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2050414.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6708..7196)
                     /locus_tag="U4B05_001263"
     CDS             complement(6708..7196)
                     /locus_tag="U4B05_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2050415.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7273..9969)
                     /locus_tag="U4B05_001264"
     CDS             complement(7273..9969)
                     /locus_tag="U4B05_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2050416.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10397..11470)
                     /locus_tag="U4B05_001265"
     CDS             complement(10397..11470)
                     /locus_tag="U4B05_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2050417.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11467..12294)
                     /locus_tag="U4B05_001266"
     CDS             complement(11467..12294)
                     /locus_tag="U4B05_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050418.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12295..13101)
                     /locus_tag="U4B05_001267"
     CDS             complement(12295..13101)
                     /locus_tag="U4B05_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050419.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13113..14198)
                     /locus_tag="U4B05_001268"
     CDS             complement(13113..14198)
                     /locus_tag="U4B05_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050420.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14215..14757)
                     /locus_tag="U4B05_001269"
     CDS             complement(14215..14757)
                     /locus_tag="U4B05_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2050421.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15015..16004
                     /locus_tag="U4B05_001270"
     CDS             15015..16004
                     /locus_tag="U4B05_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2050422.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16001..16738
                     /locus_tag="U4B05_001271"
     CDS             16001..16738
                     /locus_tag="U4B05_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN2050423.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16735..17286
                     /gene="coaC"
                     /locus_tag="U4B05_001272"
     CDS             16735..17286
                     /gene="coaC"
                     /locus_tag="U4B05_001272"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN2050424.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17302..17934
                     /locus_tag="U4B05_001273"
     CDS             17302..17934
                     /locus_tag="U4B05_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2050425.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18019..18666
                     /locus_tag="U4B05_001274"
     CDS             18019..18666
                     /locus_tag="U4B05_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2050426.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18671..19654
                     /locus_tag="U4B05_001275"
     CDS             18671..19654
                     /locus_tag="U4B05_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN2050427.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         6062 a         4238 c         3442 g         6007 t
ORIGIN      
        1 atttatttta tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga
       61 ttatgaagtc aacatttcga aaaatgaaca tacattggta agcaactata actattacca
      121 cagacataaa gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca
      181 aggtatacag cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat
      241 ttaatgaaaa caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat
      301 ccaacttcat ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa
      361 acattcatta attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg
      421 attattccgg cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt
      481 tcaacatatc catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct
      541 tgtttcatta caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg
      601 tatgcacatt gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa
      661 ttctggcctt ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa
      721 caatcttggt ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac
      781 atatgcccca tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca
      841 cctccgatac ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca
      901 gcaacatatt caccatagca agccgcattg acatctgttg tccattcaat cggaatattg
      961 aaatattgct ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat
     1021 gtgatatgac cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc
     1081 ccgatgcctg ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt
     1141 tcaactggag ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg
     1201 actgcacaga caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc
     1261 tcccttcgaa tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat
     1321 gacgtttagt atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt
     1381 cttgccgctc aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat
     1441 cttcggtaaa acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg
     1501 cgtcgtcacc aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg
     1561 catctctatt tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg
     1621 gacgtccgaa ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac
     1681 ctgttgaggt tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt
     1741 cgccccacca ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt
     1801 gaccctttaa tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat
     1861 gccaatttgc gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta
     1921 tcgtataaaa aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg
     1981 atgtttggaa aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg
     2041 gcaacgagtt cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac
     2101 tttcaacagc agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa
     2161 caaaactgcc aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg
     2221 ttccagtaat atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac
     2281 ctttgcggct tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg
     2341 gattttcggg agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta
     2401 tactcatctt tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt
     2461 tttcgtatca cttattttca atggcttttc tgacaaattt gaaatactca actcggattg
     2521 cgtacattca atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa
     2581 attatcaaca ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact
     2641 gatcattagg ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg
     2701 cacgacttct ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa
     2761 atctgccaaa caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc
     2821 gctcattaaa acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat
     2881 gcctttgatt tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt
     2941 ctgatataaa tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga
     3001 ttcttctcca tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa
     3061 gattaaccct tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc
     3121 ctcatcaccg attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt
     3181 acaaattgat tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg
     3241 ttctagggct tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct
     3301 gccggtatga tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat
     3361 tggttcccct gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag
     3421 aacctgcgga caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc
     3481 cagtaacgca ctggataacg aattactcag agaatgattg atacctcctg taaattttga
     3541 accaggaaac ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg
     3601 ttcaaacatg gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt
     3661 agcaggatag ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact
     3721 agaaaagctt tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga
     3781 cagctgccca aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc
     3841 agattcctga aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa
     3901 gcttgccact tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt
     3961 ttgctgatcc gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct
     4021 ttcgtgtgtt tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc
     4081 cagtatggga gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct
     4141 aggcaaacag tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta
     4201 gacaatcccg tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt
     4261 gacccaaaca cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg
     4321 aatcgtcccg caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa
     4381 tccctttaca tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc
     4441 gtcgtttaac tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt
     4501 catcttttcc ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg
     4561 attccaagta atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg
     4621 ccgacttttt tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct
     4681 ttaaaaatac tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag
     4741 ctcaaaacgg tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc
     4801 aagatactag cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata
     4861 tatttagagc ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt
     4921 atattgaaca taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg
     4981 atcgctagtc ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca
     5041 gccaatggtc ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct
     5101 ttttcagaat tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga
     5161 atggtcgtcg aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt
     5221 ttgtagaaat gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc
     5281 accggattca ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt
     5341 ttctcatctt tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt
     5401 caagcgttgc atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt
     5461 gtagatctct ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca
     5521 atggtaactc gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt
     5581 tactccaaag catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga
     5641 ctgttaatcc gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg
     5701 ctccaaataa aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca
     5761 taaagaagtg cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt
     5821 tagcagccat cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata
     5881 ctgctagtgg aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga
     5941 acgctgtagc cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga
     6001 cgacacccat aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc
     6061 ccatcaacgc tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga
     6121 acatttcatg tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca
     6181 taatttttct ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc
     6241 catcgccggt gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc
     6301 ttgaatttct gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg
     6361 cggtgtcatt ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt
     6421 cgcaactttt accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc
     6481 ccgcggatct tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt
     6541 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
     6601 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
     6661 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
     6721 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
     6781 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
     6841 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
     6901 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
     6961 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
     7021 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
     7081 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
     7141 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
     7201 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
     7261 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
     7321 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
     7381 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
     7441 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
     7501 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     7561 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     7621 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     7681 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     7741 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     7801 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     7861 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     7921 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     7981 attgattgaa tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt
     8041 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     8101 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     8161 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     8221 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     8281 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     8341 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     8401 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     8461 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     8521 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     8581 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     8641 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     8701 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     8761 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     8821 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     8881 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     8941 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     9001 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     9061 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     9121 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     9181 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     9241 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     9301 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     9361 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     9421 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     9481 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     9541 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     9601 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     9661 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     9721 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     9781 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     9841 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     9901 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
     9961 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa
    10021 aataatttat ctaccaattt agtttttatc taatatgatt tttatgtttt ttcatagcta
    10081 caaatattga actggattca tatattagtt ttattaatag attacagaag ggttaagtat
    10141 atttgcatag tttccaatta ctttatcctt ctgtaacttt ttcaatctta gatttactag
    10201 cccgtctcgg gccctatttt acaatggttt tgtccacttt tctctggtag aaaaacgtgg
    10261 tagaaaaaat aaaataacac aaaaaaagcg gcgtttcatc cgcttttggt agaagattgg
    10321 aaaactcaga tagaaaaagg acgattttta gctctttaga acgttgataa gtccccattc
    10381 ttactgagtt ttgccattat ctcctataca tcttgaattc taagaatagg tcattataga
    10441 tgcctaggta tttcgatcct agatctttgt agacttcaag atgggagatc gtgtcatcag
    10501 atggatagaa ttgttcgtcc gacgaaatgt cttttggcaa taacgctttt gcttcttcat
    10561 ttggtgtaga ataaccaata tactctgcat tttgtgccgc attttccggt tctagcataa
    10621 agttgatgaa atcataggca ccttctttat ttttcacggt ttttggcata acgatattat
    10681 caaaccaaag attggatcct tctggtggga tcacataatg taaatgttca ttttcccaca
    10741 tcatatcggc agcttcgcca gaaaatgtca ctgccacgga ggcttcttca ttgatcatat
    10801 acattttgat ctcatctgcc acgattgctt tgacatttgg tgttaaatga ttcaatttat
    10861 ttgctgcttc tgtcagctgt gccatattct tactattcaa agaatagcct aaactattca
    10921 aagaaagccc cataacttct cgtgcaccgt caatcagcat caaactgtct tttaattccg
    10981 gcttccacaa atcatcccaa tgcttaattt gatttgcatc aaagaattta tcgttatata
    11041 cgatccctaa agttccccaa aaatatggaa tcgaatactg attatctgga tcaaaatcca
    11101 agtctaaaaa tcgtggatca atattattta atcctttgat cttcgaatga tctaatggaa
    11161 gtactagttt ttctttgatc attttttgga tcatatattc acttggtata gcgatgtcgt
    11221 agtttgtccc accttgctgg atttttgtga acatcgcttc attagaatca aatgtttcat
    11281 aattcacttt gtatccatat tctttttcaa atttcgtgat caaactagga tcgatataat
    11341 ctccccagtt atagatggta agtacctttg ctccactcat gccacttgtt ttttcaagtt
    11401 gacgaacacc aagcagcagc accgcaataa tgatgagaat acctaaaaac agagattgta
    11461 attttttcat cgcagccgtg ccaccccctc gcgtactctt ctgtttttct tcagccgttt
    11521 tggacggttt tctttactaa taaagtagta tccaatgacc aatatcatgg agaacaagaa
    11581 aactaacgca cttagcgcat tgatttctaa actgattcct tgtcttgcac gagaatagat
    11641 ctctactgag agcgtcgtaa agccatttcc tgtgacgaaa aatgtaaccg caaaatcatc
    11701 aagagaatag gtaaatgcca taaaatatcc agctatgatt cctggagaaa gataaggcaa
    11761 aatgatattt ttgactactt ggaaattatt agcaccaagg tctctagccg catcgaccat
    11821 tgaatcgttc atttcctgca attttggcaa gaccatcaag acgacgattg gaatgctgaa
    11881 agcaatatgg gaaagcaata cactctgaaa acctaatccg aatcctagta gtgtaaagaa
    11941 aatcaagaaa ctagcaccga taataacatc aggagagact aataagatgt tattcatgct
    12001 caacaacgct gtccgtgcac gtcgtttttt cgtgtaatag attcccatag ccccaaatgt
    12061 cccgatgatc gtggcgatca atgcagataa gaaagccagc atgaacgtat tcaatacgat
    12121 aatgatcaaa cgtgtatctt caaaaacagc ttcataattt tcccaagtaa atcctgtaaa
    12181 gctgttcatc gttcctcctt cattgaagga ataaaaaatc agatagaaga tcggtgcata
    12241 taagaataaa aagacgatcc ctaaataaag atacgaccat ttaaattttt tcatttatga
    12301 gcgacctcct tttttcttct cgcctgtcag gagcatcacg atgaacatag caatgattaa
    12361 aatcacaccg attgtagaac ccatccccca gttttgtgta accaagaaat gctgttcgat
    12421 agccgttcct aaagtgatca cccgatttcc gccaatcaga cgagtcagca tgaataaaga
    12481 aagagaagga ataaatactg cttggacacc acttttcaca ccatttaatg ataacggaaa
    12541 aatcactcgt cggaaggtct caaagctatt cgcgcctaaa tctctactag cactgatcaa
    12601 agaaggattc atctcttcca atgcattgaa aattggcata atcataaatg gaatctcgat
    12661 atacgttgcc acgaacagaa aactgaagtc tgtgaataat atctgtttcg gcccgatccc
    12721 gataaaatca aagaaatgat tcacgctgcc gtgcacactg aaaatcccaa taaatgcgta
    12781 agctttaagc agcagattca cccatgtagg caaaataatc agcatcagcc ataattgctt
    12841 gtgcttcagt tttgttaaaa aataagcagt cgggtaactg atgatcagcg tgaaaaacgt
    12901 gatcaaaaat gcataccata cagaattgat cgtcatagaa agcgatgtcc ccgacgtaaa
    12961 ataagataga taattattca gcgtgaattg cccattcata tcaaaaaaag attgataaat
    13021 gatcattaat acaggcgcga tcacgaataa accgagccac aaaatataag ggacggaata
    13081 gatcctgcgc attgttttca ttcttgtccc ccctattctt cgtagctgtc aagacgtgca
    13141 tcgaattctt cttcagactc gttgaaacgc atgacatgga tatcttctgg ctcaaaggcc
    13201 aatcctactt tgcttccttc tttagctttt ttcgttgaat ggaccatcca ttcattttgc
    13261 tgttcatcat aacagatgat ttcataatgg acaccacgaa acagctgagt atcgaccgtt
    13321 acgactagtt ttcctttatc tggtgtcgtg atcgtcaaat cctctgggcg taaaacaact
    13381 tcaactggtt catttagacg catcccgcca tcgacacatt caaattgctt accaacaaac
    13441 tcaactagat tatcttcgat cattacaccg tccacgatat tgctctcccc aacaaaatca
    13501 gcaacgaaat gattgattgg ctcatcataa atatctacgg gtgtaccact ttggacgatt
    13561 ttccctttat tcatgacaaa aatctcatca ctcattgcca acgcttcttc ttggtcatga
    13621 gtaacaaaaa taaacgtgat tcctaatcgt tgttgcagat cccgcagttc atactgcata
    13681 tctgtacgta gtttcaaatc aagcgccgat aacggttcat ctaatagcaa tactttcggt
    13741 tcatttacaa ttgctcgtgc aatcgctaca cgctgtcttt gaccaccaga catttcacta
    13801 atctcccgtt tttcgtatcc aggaagctgg accatacgca aagcatcttt taccttcttt
    13861 tcgatatctg atttggacat tttcttgatc ttcaatccaa aagcaacgtt atcaaaaaca
    13921 ttcatatgag gaaaaagtgc ataatcttgg aagactgtat ttacttgtcg cttattagct
    13981 ggcaaatcat tcaaccgttt cccttcaaaa tagatgtccc cttctgtagc ttcggtgaat
    14041 ccagctataa tacgtaggat cgttgtttta ccacagccgg aaggaccaag cagtgtataa
    14101 aattttcctt gttcgatctc aaaacttact ttttttaata ctgtttcatc atcatagcgt
    14161 ttcactactt gatcaaatga aatgattggt ttccccacgt ttttaccccc tatattacaa
    14221 atatgattgc gttgcaacaa tcaatacttt tgttcgtttt ttgagatgat tccttaattg
    14281 atgttgtttt gtcgcttgat aatagatcga ctgtcctgct tttgccacat agaccgtctc
    14341 tcctaagctt aactctaatt ccccttctaa tacaaaaata aacgtttcag ataatgaagg
    14401 ttcaaacgtc ttatattctc ctgcaccttc aaaagtcagc aagacaggct ccatttcttt
    14461 ttcattcgaa tcagccacga gccattccaa ttcatacccg ttttcttcat cgaaataaac
    14521 cgtatgatct tcttttgaat aaaccacttg cagattcgct gcttcttgat gaaaaaattc
    14581 ttctggagtc acacctaaga cttcaagaat agaaaagaat gtttccattg atggtgaact
    14641 taaatctcgt tccaactggg aaatataccc ttttgacaaa tctgtgcgtt cgcctagttc
    14701 ttcttgtgtc aaattctttt ggatgcgcaa atttcttaac ttctcaccaa tttccataat
    14761 ttcctccgaa tcgaagtttt ctaatagtaa acttcctgtt tactaccact cgtattagta
    14821 tacgaaattt attgaaaaag gcaacccttt gtaaaatttt ttttgctgaa tatttcttta
    14881 tcttttggaa taaattctag ttgtttgatt ctttattttt cacctaattt ttgtctaaat
    14941 ttacggaaga acaagaataa aagagtaggc tttatggtat gataaaagaa aattttaaaa
    15001 ggagtgtctc acgaatgaac gcttttcgat cttttcaggt aataaaggaa cctgttttcc
    15061 atacagcaat cagtgatctg ccatttactg agttggctga aaatgaagtc ttggtaaaaa
    15121 ttgcctattc tgatgtcaat tataaagatg cattggcaat gagcgagtct ggtcaggtga
    15181 tacgaacata tccgatgact ccagggattg atttatccgg aactgtcgtc caaagtaaca
    15241 atccacgttt ttcaaaagaa gatcttgtac ttgctactgg ttttgggctt ggcgtcaccc
    15301 atccaggcgg ttatagccaa tatcaaaaag taccaggaga ctggttagtt cctctaccaa
    15361 aaaatatgac gctgcgtcaa gcgatgatcc ttggtacagc agggtttact gctatgttgt
    15421 gtgtgaacgc tttaatccgt caaggtatga cctccgataa aaaagttgtc gttactggtg
    15481 cttctggcgg tgtaggcagt acagcgatag ccctgttaca taaactagga ttccagtcaa
    15541 tcattacctt ttccagaaaa gaagagtctg tcacatggct aaaaagtcta ggtgctttgc
    15601 aagtcgtacg acctgatgaa tttctgccag agacaacaaa agctttagga aaacagcaaa
    15661 tcgactatgt catcgacaca gtaggtggcg agcagctaag cagtcttctt ccattgatct
    15721 cttataacgg tgcagtagct ttatgtggca atgctggggg aatcaaacta aacgcgactg
    15781 tcttgccttt tattctgcga aacatccagt tgattggaat tgattctgtc aatgtaccaa
    15841 tagatcaacg tctttcttta tggcaacaga tggcggattt gcagattgca gatgaactgg
    15901 tcgtccaaga gattactctg gatcaattgc ctgaaacagc atctaaacta cttgctggaa
    15961 cacaccaagg aagaacacta gtaaatgtag gtgaccataa atgaagattc taatcacagc
    16021 tggaggaacg actgaaaaaa tcgatcaagt acgtgctatt acaaatcatt cgacaggacg
    16081 attagggcaa gcattagctg attatttagc tgccaatccg gataccacaa tcgattacgt
    16141 gactacgaaa caagcactga aaccgaaaag acactccaat ataacgattt atacgatcga
    16201 atctgcactg gacttatttt tacaattaga agaactaacg aaaaaagaac actacgatgc
    16261 gattatccac agtatggcag tcagtgattt tactcctgct tttagttttt cagaagaaca
    16321 gctggcaaaa aacctaccca cttcttcaac gcaagaagaa ctggacaatt ggtttgcgga
    16381 aaatgaacag acagatacca ctgtttcaaa gatctcttct aacaccgagc atcttgtgct
    16441 cgttttgaag aaaacaccaa aaatcatttc ttatctacgt gaatggcagc caaaagcaaa
    16501 aatcattggt ttcaaactat tagtagatgt accaaaggaa tctcttctgg cagtcgcaaa
    16561 aaatagtctg ataaataaca aaactgattt tattttcgca aatgatttga cggaaatcca
    16621 tggcgagaca caccacggct atctcttatc taaagatgga acagtggaag aagctcaaag
    16681 taaatctgag attgctgcat tgattactga gaaaattaga ctggaggaat caaaatgaaa
    16741 aagatcttac taggcgttac tggaagtatt tctgcttaca aaagcgctga tcttacaagt
    16801 caattcgtca aattaggtgc ccaggtagat gtgattatgt ctaaaagcag cacacatttt
    16861 atcacgccat taaccttgca atctctgtcg aagcgcgcag tccatacaga cgtcatgcaa
    16921 gaatcaaatc cgcaggtaat caatcatatc gaactagcaa aacaagctga cttgtttctc
    16981 atcgcgcctg ctacagcaaa cattatcggc aagctagcaa acggcattgc agacgatctt
    17041 ctttcaacag tcgcaatggc gcttccagtg ggaacaccaa aattgattgc ccctgccatg
    17101 aacacgaata tgtatcaaaa cccgatcact cagcgtaatc tggaaacatt gagatctgtc
    17161 ggctatcagg aaattgaacc aagagaatct cttttagctt gtggagattt tggaaaaggt
    17221 gccttagctg atattcagac gatcatcgaa caggtcatgc tgactcttaa tgaaagaaat
    17281 gcttagaaaa aaggagtact tatgaaaaat acaaaaaact ttacattaac tgcgatgttt
    17341 ttagctattt tgattttatt agctgttacg cctttaggtt ttatccctat cggtccaatc
    17401 aatgcaacaa ccatgcatat tcctgtaatc attgcttcga tcgttttagg accaagacta
    17461 ggtgcttttc ttggcggtac atttggactg atcagcatga tccgtagcac agttatccaa
    17521 acacctttgt cttttgtctt ttctccgttt atcccagtca tcggaacaga acatggaagc
    17581 tggaaagcct tgctgatcgc gtttattcct cgcatcttga ttggggtggt tccttatttc
    17641 gtttataaag gtctgcgcaa actaatgaaa caaaaagcag attccgtttc tttattctta
    17701 gctggacttt ccggttcatt gaccaatacg attttagtga tgaatatgat ctacttttta
    17761 ttccagcaag attatgccaa agtcatcgga acaaatttaa atgctgttta ttcagctatt
    17821 ctagcggtta tttttacaag tggtatacct gaaggaatcg tcgcaggtct tgcgactgca
    17881 gcagtatgca atgtcttgtt aagatacttc aagcaccctt ctcttactaa ctgatagtca
    17941 tcgtagccga tctctgattt ttttctcttt tggtgttact attgaatgga aagatttgac
    18001 gtcgaaagga tgatccaaat gaaaattttt gttgtaggtg caaacggaca aattggccga
    18061 cacctgataa aagatttagc tccaagttcc catgaaattt ttgcaggggt gagagatgtt
    18121 gcttcccaaa cattagtaaa aaaagagaat gtttcgtatg tctcttttga tttaacttgg
    18181 tccgtggaaa aaatgaccga agcattcaaa ggcatcgatg tattgatttt tgctgccggc
    18241 tctcaaggaa agaatcttct gcaagtagac ttggatggcg caatcaaaac agtgatcgca
    18301 gctgaaaatg cgcatgtctc tcgctatctg atggtcagtg ctgtttatgc agatgaacca
    18361 gcaaaatggc ctgagtccat gacagattat tatattacta aacattatgc agatgaatgg
    18421 ctgaaacgaa ctaacttaga ttttgtcatt cttcaaccag tcacattgac taacgatgaa
    18481 gaagttactt ccattcaatt aactaagcct aatgagaagg catcaaaaac gatcactcgc
    18541 tctacagttg ctgctgtact tgccgctcta gtggaagaaa cggatatttc tcgtacgact
    18601 ctagtacttt ctgaaggtag caaagagttg aatacagcat tccaagaatg ggcaaaggag
    18661 gaatagatcc atgcgtgcca tttcaatcaa gcatcctggt tctgtagatg tattagaaca
    18721 aattgaagtg cctgttccta aaccaaaaca aggcgaatta ctgatcaaag tccatacagc
    18781 agctgtcaat cgaacagata ttatgaaaag agaatcagtc tctttgaaac ctccctaccc
    18841 tattttggga gtcgaagtag ctggagaggt agttgagaat aaaagtgata tctccacttt
    18901 tactcctggg acaaaagtat acggattagt caatctaggt ggttatgccg aatatgctgt
    18961 gatgcctgcc gatcgtgcaa tccttctccc tgatacgcta gactatgttt ctgctgctgg
    19021 aatctcagaa gtttttttga ctgcttatca aacactttac tggcttggaa aattacaaga
    19081 aggcgaaact gtcttgatcc atgcaggagc aagtggtgtg ggaacagcag cgatccaatt
    19141 agctagacag ctttctcacg cgaaaatcat cgttactgca ggatcagaag aaaaactcgc
    19201 cttttgtaaa gaacttggtg cagatgaatt gatcaactat aaaaaacaag actttcaaga
    19261 agaggtacga aaaatcactg aaggtcgggg agtcgatgtt attttggatt tcatcggcgc
    19321 ttcttactgg gaaaagaatt tagcttctat agccgttgat ggacgttggg tcttgattgg
    19381 tatgctaggt gggaccatcg tgcctgagat agaccttggt gtattgattg ggaaacgtgt
    19441 ccaactgctt ggtacattat tgacacctag aagtgatcag tataaagcag agctcgcaca
    19501 aacttttatg caaaaagctg ggccttttct agaaaatggc aagattcggc caatcattga
    19561 ccgtacattc caattcagcg cagtcaaaca agcgcatgaa tatatggaag caaacaaaaa
    19621 catcggaaaa atcattttga agataactga ataaaatcat gctgatcaaa aaagaggctg
    19681 ggacagaagc gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt
    19741 tttgtgaat
//
LOCUS       DASJST010000044        19502 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJST010000044 DASJST010000000
VERSION     DASJST010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.044"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(455..1084)
                     /gene="udk"
                     /locus_tag="U4B05_001276"
     CDS             complement(455..1084)
                     /gene="udk"
                     /locus_tag="U4B05_001276"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN2050428.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1168..1863)
                     /locus_tag="U4B05_001277"
     CDS             complement(1168..1863)
                     /locus_tag="U4B05_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN2050429.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1876..2592)
                     /locus_tag="U4B05_001278"
     CDS             complement(1876..2592)
                     /locus_tag="U4B05_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN2050430.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2922..3527
                     /locus_tag="U4B05_001279"
     CDS             2922..3527
                     /locus_tag="U4B05_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN2050431.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3852..4175
                     /locus_tag="U4B05_001280"
     CDS             3852..4175
                     /locus_tag="U4B05_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN2050432.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4357..5727
                     /locus_tag="U4B05_001281"
     CDS             4357..5727
                     /locus_tag="U4B05_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN2050433.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5857..7158)
                     /locus_tag="U4B05_001282"
     CDS             complement(5857..7158)
                     /locus_tag="U4B05_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2050434.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7388..9346)
                     /locus_tag="U4B05_001283"
     CDS             complement(7388..9346)
                     /locus_tag="U4B05_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN2050435.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9523..9789)
                     /locus_tag="U4B05_001284"
     CDS             complement(9523..9789)
                     /locus_tag="U4B05_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2050436.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10299..12425)
                     /gene="pnp"
                     /locus_tag="U4B05_001285"
     CDS             complement(10299..12425)
                     /gene="pnp"
                     /locus_tag="U4B05_001285"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN2050437.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12613..12882)
                     /gene="rpsO"
                     /locus_tag="U4B05_001286"
     CDS             complement(12613..12882)
                     /gene="rpsO"
                     /locus_tag="U4B05_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN2050438.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13133..13696
                     /locus_tag="U4B05_001287"
     CDS             13133..13696
                     /locus_tag="U4B05_001287"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2050439.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13949..14470)
                     /locus_tag="U4B05_001288"
     CDS             complement(13949..14470)
                     /locus_tag="U4B05_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN2050440.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14473..15315)
                     /locus_tag="U4B05_001289"
     CDS             complement(14473..15315)
                     /locus_tag="U4B05_001289"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN2050441.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15308..16033)
                     /locus_tag="U4B05_001290"
     CDS             complement(15308..16033)
                     /locus_tag="U4B05_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN2050442.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16209..16505
                     /locus_tag="U4B05_001291"
     CDS             16209..16505
                     /locus_tag="U4B05_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050443.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17073..18665
                     /locus_tag="U4B05_001292"
     CDS             17073..18665
                     /locus_tag="U4B05_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2050444.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18678..19418
                     /locus_tag="U4B05_001293"
     CDS             18678..19418
                     /locus_tag="U4B05_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2050445.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3697 g         6223 t
ORIGIN      
        1 tgcgacaaaa gagcttcttc aaaaaaataa gtcgaaatct cacacaaatt cgagaaataa
       61 ttttcgtaag tttcttctta tttattgcat ctgaccactt ctgttacaac ctctcgttaa
      121 acggtgttca aaagcttgca tctacgataa taagcccaac ccaacgaaaa acgtgaggag
      181 ctgttttatt tggatgctct tatcgcttga tgcaaagaca gcttttttac aacctctact
      241 tcacggtgct caatcagctg accttcggta ttagctcaaa aaacggtaaa atataaacag
      301 ctattttccg tttcttcttt ttaatactta ggtcaagatg ctgttttcac aacctcttgt
      361 tataaataaa aaaaacagtt ggctggtgac tgttcaaaaa gcagaatcct tgcttttcaa
      421 acggtcgcag tccgttcaac tgtttcgctg cgcactattt atggtttaag aaactagcta
      481 cttttgttgt aatcaaatca atagcaacgt ggttttctcc gccttctgga acaatgatat
      541 ccgcatagcg cttcgttggt tcgataaatt gatgatacat cggtttaaca actgtcaaat
      601 attgttcgat cacggagtcc aatgtgcgtc cccgttcttc catatcccgt ttgatacgac
      661 gaatgatacg aatgtcatca tctgtatcta cgtatacttt gatatccatc aagtcacgca
      721 gacgttcgtc ttctaaaatc aggattcctt ctagaatgat cacttctttt ggttcctgaa
      781 tgatcgttgc ctgacttctt gtatgcgcta catagtcata cacgggtttt tcaatggctt
      841 tatagttcag taagtcgcct acatgctgaa tcagcagatc attatcaaag gcaaatgggt
      901 gatcgtaatt tgtgttcaat cgttcttcaa aactcaaatg gctctgatcc ttgtaataag
      961 aatcttgttc aagcatcatg atggaatggt ccgggaagtt gttgaaaatc gcacggctga
     1021 cacttgtctt tccgcttccg gatccacccg tcacaccaat gataattggc ttgctttttg
     1081 tcattgacta aaacctcttt cgtatccgtt ttttcattct aatctattat agcgaaaaaa
     1141 cacgaaaatg ctatattttt ctgaaaatta atccttttca atttccaata atgaataaag
     1201 ttcctctaat gaatcgattc gataagtagg ctggatttct tcgatttctg gtaatttatt
     1261 tctattgaac caaatcgtat cgatccctgc catatttcct cctttgatat cagaagtcaa
     1321 agaatcacca atgatcacag tttgcttttt atcaaaatta ggaatgtggg aaaagacatg
     1381 atcaaaaaat tctttcatcg gcttttgcgc tccgacagct tctgagacaa agatatcctt
     1441 gaagtaatca agcattcctg catctgttaa tcgccgatgt tgcgtgtgcg acacaccgtt
     1501 cgtcacaata taaagatcag catgcggatg gacctttttc aggatttcta gacttcctgg
     1561 aacaagatca tgaccttggt tcaagtagtg gcggtagcgt ttttccattt gatcactatc
     1621 tactgttttc ccaaattgct ggaaaagtaa cccaaaacga ctgcctatta tttcttctcg
     1681 tgatagtctg cctttttcaa actcctgcca taaatgatga ttcaaagcct tatagctggt
     1741 ttcgatttct ggtgtcaatg tgattccttg ttctgcaaac aaagcactta aagcattggc
     1801 ttcagttaat tgaaaatcta acaatgtatc atctacatcg aacaataaag ttttatattt
     1861 catctttttg acctcctatt taaaataaat acttaacttt ttcatctctg ctaatgtcaa
     1921 cgatcgatac tcgcctactc ctagctgttc atctaattcc aatggtccca tgcttagccg
     1981 tttcaatgcg gtcactttct ttccttgggc taagaacatc tttttgactt gatggaattt
     2041 tccttcttgg atcgtcaata aaaccgtact ttggtgcttg tctgctttga gtatttttaa
     2101 atcagccggc ttacattgat acccatcagc gaatacgatt ccattttcaa atgctgttac
     2161 gtctgccggt gtcacccttt cgttcacaac tacttgataa gtctttgaaa cctttttttc
     2221 tggaagtagc atttcatatc ccaattgtcc attcgaagtg atcagcaata gtcctgttgt
     2281 atctctatct agtcttccca ctggatataa tggttggaaa cgatcggcac ttgcaatcag
     2341 atcgattact gtcgtctttt ctggatctga tacagctgta acagcttttt caggtttatt
     2401 gagaagataa taggactcat ttgtttctaa gtgttcacct gctacttcga tttgatgcag
     2461 taaactatct acatttcgat tttgtcgaaa ttcgactgtg ccatctatca tcacttgctt
     2521 caaagcaaac agccgcttca tttccttgcg tgtcgtttct aatttttctt ctattaattt
     2581 atctaaacgc atgctgtttc ctttcttatt actactaatt aattatacct tgaatattct
     2641 tgcttgcaaa tacatcatca tagttattcc ttgacttaat taacagttca cgttacggtt
     2701 aaatagtaaa gtgaattgga tttctcatac acagcgtaat gacctggtgg agaaaagttg
     2761 aatcctgact ttctaagggg agatgcgcgc atctctcctt ttgcgctttt ttaagcgccc
     2821 tgccttcgat gatgtgcacg agattagcct ttagaattca cttaaataaa atctgtccgt
     2881 taccaaagca gacagtcaca ttttaaggag gaattttggt tatgtcagta tcattagaag
     2941 taagtaaaag agaagtacgt ccccgttcat taagaaacaa acttcgtcac gaagggaaag
     3001 ttccagctgt tgtttatggg tataaagtag aaagtacccc tatctcattc gatggtttga
     3061 cactaagcaa attgttgcgt gaacatggag cgaacacagt catcacgatg gacatcgatg
     3121 gacaaaaagt caatacattg gttcacaaga tccaaacgaa cacttttacc aatcatttcg
     3181 aacatgcaga atttctatct gtaaacatgt cggaagaaac agaagtcgaa acagaagtcg
     3241 ttcttgttgg cgaatcagtg ggtgctaaat ctggtggagt gttagctcaa aacttgtata
     3301 cagtagtcgt atctgctaca ccagacaaat tgcctgaacg aattgaagtt gacgtgacaa
     3361 acttggaatt aggtgattct attacagtag ccgatcttcc aaaaaacgac gattacaaat
     3421 tcgtgaccga cagtgaagag caaatcgctg ctgtagtcgc tcaaagtgaa gaagaaagca
     3481 caggtgaagg tggcgaacca gaagtgatcg gttcaacaga agaataatac tgaaacaatc
     3541 aggtggtgtt agaagcggcg ggtcagcagc ttttaacacg ccttttttat ttctaatcgt
     3601 ttattttcat atggggcttc cacttttgta aatggcttta caaacgaaaa atcctaattt
     3661 accaagcttt tcctcgcata cttttatcgt acctttataa aaatttatac tttgaaaaaa
     3721 agtattcaat tattgttgca tttttcctga ttctttatta gaatgaaccc tcgttgaagt
     3781 aagtatttct agtttatcct aggattaatt ataaaaataa aacattcgtt gattcgaagg
     3841 aggttaaaca gatgtttgaa agttttgata gcagccgcaa ccgttacgct tcagttggtg
     3901 tcgtatcaag tttgccagat gaattgattg acagtatatg gtttattatt gacctagatc
     3961 ttaagggagt cattccatta gaaaatatat taacctttga tttgatcgaa aatcgtggaa
     4021 aagtgacgat gcatttttct caagcaggaa gcaatgtgga aatggatatt gatttgccgt
     4081 ttgcctattc gaaccaattt cctgctcaag tatacgccta tgatgacggt acgagagaaa
     4141 cgatcctttt accaagtgag attagacaat attaaggtga tagataaacg tggatgtccc
     4201 ttattttaca gaccaggcca agttgttgat tctttgactc aattcttggc ttctttttat
     4261 ggagtttcat aaattatcat tgatttctaa ttggtattct caggataaac gctatatact
     4321 actaactaaa ttttaaagat ataaggagtt taaaagatgc gcgatttaac aaaaggaagc
     4381 cccgctaaac tgattttgat gttcactgtt cctttactga tcggaaatgt ttttcagcag
     4441 ttctacaata tgatcgatat gatcatcgtg ggtcagacgc taggtaaaaa tgcgctggca
     4501 gcggttggtg caactggaag cctgacattt ttgatcattg gctttgccca aggtttaaca
     4561 gctggtttag ccattattac cgcacaacgt tacggtgcaa aagattatcg gggtctgaag
     4621 aaaagttttg ctgctagtgt tgtcattagt cttgtcgtca ctgtgatttt aactgtccta
     4681 agtctcctgt ttattcgtcc gatgcttcaa ttgatgcaaa ctcctcctga aatacttgat
     4741 caagcacaaa catttatttc aattatttta ttagggatct ttgcatcagt cagtttcaat
     4801 ttgctttcta atgtcattcg cgcacttggt gacagccgca caccactatt ctttctgatc
     4861 attgcggtca tcatcaacgt cgtacttgat ttgatcttta ttatctattt tggcatgggt
     4921 gtggaaggtg cagctattgc tacagtaatc gcacaagttt cttccagcgt actgtgtcta
     4981 gtgtatatca agaaaaaaat gcctttgtta cagttacgca aaaaagattt caaatttgaa
     5041 aaagaagagt ttgctgtaca cctaaatgct gctttgccaa tggcgtttca atcttcgatc
     5101 atcgctgttg gtgctatcgt gttacaagca gcactaaata gtctagggac agatgtcgta
     5161 gctgctcaag ccgctgctgg tcgtatcgac caatttgcaa atcagccgat gatgtcattt
     5221 ggtattgcaa tggcaacttt cactgcacaa aactatggtg cgaaagaata tggccgaatc
     5281 ttgcaagggg tacgtcaaac tttactgatg agtgtcggat ttagtctctt agcaggtgct
     5341 atcgtcattt tcttcggtca tttctttgtt caattgtttg ttagcccatc agaaacacgt
     5401 gtatttgaat tagcacaaac ctacttcaat atcaatgggt ctctttactg gatcttagca
     5461 gtcctgttca ttttacgcta cacacttcaa gggcttggac aaagcaagat ccctacgatc
     5521 gccggtatga tggaattgct tatgcgttct tttgcagcta tcgttcttac aggaatgtgg
     5581 ggatacccag gagcagcagc agcttctcct ttagcttgga tcggttctgt tgccgtgttg
     5641 ctttactctt atatgcgttc gatgaaacaa ttgaaacaca tgggtgaaga ggaacatttc
     5701 acattagaac atgcagaagc aagatagcct tgattaaaag aaatcgttgc ggctaaaaat
     5761 gaaaactttt attgaaaata tcttcttcgt ttaccgaaaa caggtttttt agataacaaa
     5821 aaggctgtga caaaaaatgt cacagccttt tttcttttat tctatttttc tttccgtgga
     5881 ctctactttg aaataatcga tatgtgcatg ctggtattct aataccgtga aacggaatga
     5941 tgcaatctca atgattgttc ctgcttttat ttctttatat cggctagaag caaagcccgc
     6001 aagtgtagta aaattcgttt cttcaaactc tggaatctct acatgaaaat aacgctggaa
     6061 atcatctaat gttagtttgc cggagatgat ataagagcca tctgactgtt ccgctatcaa
     6121 tgcttcgtcc aaatctgtct catctcggat ttccccgacg atttcttcta aaatatcttc
     6181 aatcgtcact aagccgcttg tccctccgta ttcatctttt aagatagcaa tatgctttcc
     6241 cttttttcgc atgatcgtca ataatttttt tatagggatc gtttcagttg ttacgatcgg
     6301 ctcttcaatc aactgttcta ctgtcagctg gtcatcttta taagattgct tgatcaaatc
     6361 tggcaaggtg acataaccta aaacattgtc ttttgattct aagataaccg gatagcgagt
     6421 atgtccttgt tcaatcgaag cttttatcgc ttctttgacg gtttctgaag cttcgaatac
     6481 ttgcatggac gtacggtcta cttgtatatc ttttgccaag gtatcatcaa attcaaacac
     6541 gttttccaaa taatgatatt cttctttggt caactctcct ttttcataag aattcaatgc
     6601 gactaatgtc agctcttctt gagacaatgt ctcttctcct tcagatacga gacgcacacc
     6661 taatagcttc cctagccctg cagctgaatg attcaatacc caaataaaag gaaacattac
     6721 tttataaaaa taatgaagtg gtttaactac gaacaagacg accttctccg tattgacgat
     6781 actatagctc ttaggaagca gttcgccaat aaccacatcg atatacgtaa ctaaaataaa
     6841 tcccagcgcc acagagattg tactgctaat agccgaacta aaaggtagtt taccgatcac
     6901 tggttctagt aaagccgcga cagatccttc accgacccac ccgatgatca aaccggttag
     6961 cgtaattcct aattgactag ctgataaata gttatctaaa tggtgtgtaa catgaagagc
     7021 tagcttggcg ttctttacat tttccttttc tagttgttcc aatcttgatc gtctgatttt
     7081 cactaaagca aattcacttg ctacaaaaaa tgaagtaatt gcaatcatca aaaataaaat
     7141 aatgaaattg ataaccatat tctcccgcct ttctcttttt ctattgtacc ctttttctac
     7201 aaaaagattg aaaataatgc gtattcatat ttaggaataa aaaaggagat gcggcatttt
     7261 tcagccacat ctccttatca cttcaaaatc attgcgttac ttgttgctaa acagcttttt
     7321 catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc aggtcaaagc
     7381 gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg ataagcggaa
     7441 cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc gaggtagcga
     7501 tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct agagttgtca
     7561 cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct cgccctggtg
     7621 tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa tcatggatga
     7681 cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac agattcactt
     7741 ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat ggctcatccg
     7801 tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta tctaaaatcg
     7861 aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag aagaccacat
     7921 ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc gggatataat
     7981 ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt tcccaaataa
     8041 acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct cggaagaatt
     8101 tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag gtaactaaat
     8161 atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt gctgttgtca
     8221 tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga gacgttcgga
     8281 atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct gggatataca
     8341 tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt ttcaacttga
     8401 ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca atgactgcag
     8461 cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt tggaagaatg
     8521 gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac aaccacgcag
     8581 cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc agacgaatat
     8641 ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc gtagccagga
     8701 aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc agctggatag
     8761 cataataagg attcagtgca cggataactg tccagtcttg actgaagttc attaatccca
     8821 ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt ccgacaatat
     8881 ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg ataatgacga
     8941 tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc aagccttcaa
     9001 tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag gcagctcctc
     9061 cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg tagagtgaga
     9121 aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat ttgattgttg
     9181 tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa tcaggattga
     9241 ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc ggacttgtcc
     9301 cgatgtctcc gtagacgact cccatcgcta caagcaggcc agccattgat atctttttgg
     9361 tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat cctttaggta
     9421 agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat tgcttctaat
     9481 tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt tctttggacg
     9541 ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg cacccaataa
     9601 tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta aaaattcgcc
     9661 gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg ggacttcctt
     9721 tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg accataaaaa
     9781 attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta cgctttattc
     9841 agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg taacaaaagt
     9901 gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg ttgtgaaatc
     9961 agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat attttgccgt
    10021 tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga ggttgtaaaa
    10081 aagctgttct gcatcaagta ataagaacag ccaagtaaaa acagctccta atgtttttaa
    10141 cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc gaaaaaaaag
    10201 gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca
    10261 ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt
    10321 gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt
    10381 tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca
    10441 cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg
    10501 aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc aactttgact
    10561 tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa
    10621 gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt
    10681 ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg
    10741 atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa
    10801 gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg
    10861 atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt
    10921 gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt
    10981 aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct
    11041 ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa
    11101 gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct
    11161 tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga
    11221 cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg
    11281 tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga
    11341 ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca
    11401 tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc
    11461 ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct
    11521 tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc
    11581 tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt
    11641 ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt
    11701 ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga
    11761 acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca
    11821 ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt
    11881 ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc
    11941 aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg
    12001 atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag
    12061 tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca
    12121 aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga
    12181 cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca
    12241 taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca
    12301 cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc
    12361 tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct
    12421 gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg
    12481 ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca
    12541 cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct
    12601 ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac
    12661 gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc
    12721 gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc
    12781 ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta
    12841 ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga
    12901 ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt
    12961 gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat
    13021 taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat
    13081 aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac
    13141 aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc
    13201 tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa
    13261 cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc
    13321 tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga
    13381 ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt
    13441 tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg
    13501 atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca
    13561 caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa
    13621 cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt
    13681 attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta
    13741 taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa
    13801 atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct
    13861 tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt
    13921 tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta
    13981 gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga
    14041 tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg
    14101 aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat
    14161 gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt
    14221 ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt
    14281 ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta
    14341 aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag
    14401 cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc
    14461 cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa
    14521 atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac
    14581 tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt
    14641 cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg
    14701 atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc
    14761 tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt
    14821 ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac
    14881 gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag
    14941 accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg
    15001 agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa
    15061 atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg
    15121 ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag
    15181 ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc
    15241 aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt
    15301 tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt
    15361 cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga
    15421 ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg
    15481 ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc
    15541 catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt
    15601 gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat
    15661 gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca
    15721 aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt
    15781 ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga
    15841 atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc
    15901 cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg
    15961 caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg
    16021 ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg aacaaattta
    16081 ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc
    16141 acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt
    16201 gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt
    16261 tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt
    16321 gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat
    16381 atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta atattagcca
    16441 tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag
    16501 cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc tttttttatt
    16561 gtattacata catttttgtg tataattata ctatcgtttg caatggatag atgattcaca
    16621 ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa
    16681 ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac
    16741 tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag
    16801 tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt
    16861 ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt
    16921 tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct
    16981 ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct
    17041 cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg
    17101 tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa
    17161 aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt
    17221 ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg
    17281 gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg
    17341 tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca
    17401 tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt
    17461 acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt
    17521 tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga
    17581 tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa
    17641 cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg
    17701 caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga
    17761 caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa
    17821 aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag
    17881 cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt
    17941 tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt
    18001 tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc
    18061 gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac
    18121 ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca
    18181 tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt
    18241 ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg
    18301 cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga
    18361 tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt
    18421 ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct
    18481 tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg
    18541 ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga
    18601 aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg
    18661 aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct
    18721 tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga
    18781 cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg
    18841 aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata
    18901 atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca
    18961 atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag
    19021 aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg
    19081 aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat
    19141 tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa
    19201 tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta
    19261 tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata
    19321 aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac
    19381 gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa
    19441 agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg
    19501 aa
//
LOCUS       DASJST010000045        19445 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJST010000045 DASJST010000000
VERSION     DASJST010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.045"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..3658
                     /locus_tag="U4B05_001294"
     CDS             131..3658
                     /locus_tag="U4B05_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN2050446.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="U4B05_001295"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="U4B05_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN2050447.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="U4B05_001296"
     CDS             7446..7787
                     /locus_tag="U4B05_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2050448.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="U4B05_001297"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="U4B05_001297"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN2050449.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="U4B05_001298"
     CDS             9144..11564
                     /locus_tag="U4B05_001298"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN2050450.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="U4B05_001299"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="U4B05_001299"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN2050451.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="U4B05_001300"
     CDS             12998..13735
                     /locus_tag="U4B05_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2050452.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="U4B05_001301"
     CDS             13749..14564
                     /locus_tag="U4B05_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2050453.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="U4B05_001302"
     CDS             14577..15224
                     /locus_tag="U4B05_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2050454.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="U4B05_001303"
     CDS             15240..15896
                     /locus_tag="U4B05_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2050455.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="U4B05_001304"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="U4B05_001304"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN2050456.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="U4B05_001305"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="U4B05_001305"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN2050457.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="U4B05_001306"
     CDS             18238..18756
                     /locus_tag="U4B05_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2050458.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="U4B05_001307"
     CDS             18863..19363
                     /locus_tag="U4B05_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2050459.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6536 a         3339 c         4175 g         5395 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctg
//
LOCUS       DASJST010000046        18838 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJST010000046 DASJST010000000
VERSION     DASJST010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.046"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..435
                     /locus_tag="U4B05_001308"
     CDS             <1..435
                     /locus_tag="U4B05_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN2050460.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="U4B05_001309"
     tRNA            complement(759..842)
                     /locus_tag="U4B05_001309"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="U4B05_001310"
     CDS             1370..1870
                     /locus_tag="U4B05_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2050461.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="U4B05_001311"
     CDS             complement(2060..2404)
                     /locus_tag="U4B05_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN2050462.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="U4B05_001312"
     CDS             complement(2558..3478)
                     /locus_tag="U4B05_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2050463.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="U4B05_001313"
     CDS             3665..4156
                     /locus_tag="U4B05_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN2050464.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="U4B05_001314"
     CDS             complement(4180..6555)
                     /locus_tag="U4B05_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN2050465.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="U4B05_001315"
     CDS             6881..7507
                     /locus_tag="U4B05_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2050466.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="U4B05_001316"
     CDS             7530..8234
                     /locus_tag="U4B05_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2050467.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="U4B05_001317"
     CDS             8426..9259
                     /locus_tag="U4B05_001317"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN2050468.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="U4B05_001318"
     CDS             9456..11384
                     /locus_tag="U4B05_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2050469.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="U4B05_001319"
     CDS             11522..11977
                     /locus_tag="U4B05_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN2050470.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="U4B05_001320"
     CDS             12447..12716
                     /locus_tag="U4B05_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050471.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /locus_tag="U4B05_001321"
     CDS             13116..13370
                     /locus_tag="U4B05_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN2050472.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="U4B05_001322"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="U4B05_001322"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN2050473.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="U4B05_001323"
     CDS             complement(14543..16816)
                     /locus_tag="U4B05_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN2050474.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="U4B05_001324"
     CDS             complement(16809..17348)
                     /locus_tag="U4B05_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN2050475.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="U4B05_001325"
     CDS             complement(17484..17702)
                     /locus_tag="U4B05_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN2050476.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="U4B05_001326"
     CDS             complement(17699..17944)
                     /locus_tag="U4B05_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2050477.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="U4B05_001327"
     CDS             complement(18060..18788)
                     /locus_tag="U4B05_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN2050478.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5789 a         3651 c         3312 g         6086 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaaca aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatct
//
LOCUS       DASJST010000047        18806 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJST010000047 DASJST010000000
VERSION     DASJST010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.047"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..1412
                     /gene="nhaC"
                     /locus_tag="U4B05_001328"
     CDS             45..1412
                     /gene="nhaC"
                     /locus_tag="U4B05_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2050479.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1693..2712)
                     /locus_tag="U4B05_001329"
     CDS             complement(1693..2712)
                     /locus_tag="U4B05_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2050480.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2734..3741)
                     /locus_tag="U4B05_001330"
     CDS             complement(2734..3741)
                     /locus_tag="U4B05_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2050481.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3734..4399)
                     /gene="pgmB"
                     /locus_tag="U4B05_001331"
     CDS             complement(3734..4399)
                     /gene="pgmB"
                     /locus_tag="U4B05_001331"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2050482.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4392..6686)
                     /locus_tag="U4B05_001332"
     CDS             complement(4392..6686)
                     /locus_tag="U4B05_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2050483.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6946..9111
                     /locus_tag="U4B05_001333"
     CDS             6946..9111
                     /locus_tag="U4B05_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2050484.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9415..10248
                     /locus_tag="U4B05_001334"
     CDS             9415..10248
                     /locus_tag="U4B05_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN2050485.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10350..12050)
                     /locus_tag="U4B05_001335"
     CDS             complement(10350..12050)
                     /locus_tag="U4B05_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050486.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12557..13717
                     /locus_tag="U4B05_001336"
     CDS             12557..13717
                     /locus_tag="U4B05_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2050487.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13733..14176
                     /locus_tag="U4B05_001337"
     CDS             13733..14176
                     /locus_tag="U4B05_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2050488.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14659..15189)
                     /locus_tag="U4B05_001338"
     CDS             complement(14659..15189)
                     /locus_tag="U4B05_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN2050489.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15294..15845
                     /locus_tag="U4B05_001339"
     CDS             15294..15845
                     /locus_tag="U4B05_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2050490.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15998..16471)
                     /locus_tag="U4B05_001340"
     CDS             complement(15998..16471)
                     /locus_tag="U4B05_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2050491.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16609..18081
                     /locus_tag="U4B05_001341"
     CDS             16609..18081
                     /locus_tag="U4B05_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2050492.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18078..18788
                     /locus_tag="U4B05_001342"
     CDS             18078..18788
                     /locus_tag="U4B05_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN2050493.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5760 a         3399 c         3884 g         5763 t
ORIGIN      
        1 ttttttcatg gcagggcctt ttcatttgaa ataggagatc gacgatgaat aaaaatattt
       61 ctttaaaaga aagtatcgct gtattgatcg tgttgttggc actcctagga gtactgatca
      121 tcggattcca gctctcgcca catatcccga tccttgcagc tttcatgtgt ttgttgtttt
      181 atggcagatt taaaaagttc acatgggatg agatccatga cgggatcatc aaaggaatca
      241 ctccagggat cattccaatc ctgatttttc ttttgattgg tgtcttagtt gcttcatgga
      301 ttttatctgg aaccatccct acgattatgg tttatggatt taaacttgtt tctgtgcgtt
      361 tctttttacc gacagcattt cttgtttgtg caattgtcgg agtgactgtc ggcagttcat
      421 ttacaacgat ttcgacgatt ggaattgctt ttttggggat cggtcattta cttggattca
      481 atgatgcaat gactacaggc gcagtcgttt ccggtgcctt tttagggaat aactgctcgc
      541 ctttatcaga tacgaccaac ttggcagcgg gtattggagg agttaatcta ttcgaacata
      601 tcgctggtat gcgttatacg gatttcccag cattcgtcct ttcgttgatt ttttatacga
      661 tattaggaca taacagtcat tcagccgatc tagctagtat caatgaaatg atccaaaaca
      721 tgaaagcgag cttttggatc tctccttgga cattgattcc actagttatt ttatttgggg
      781 gggcaatcaa aaaaattccg gctatcccta cattactagc gggatcgaca gctgctctcg
      841 ttctttcttt tatccatgag aaaagtttga cggtcggaaa agcagcagac atcttgatga
      901 atggttatgt cgcaaataca cctgatccaa agctagatga actgctttct cgaggcggga
      961 tcatgagtat gttaggttct gctagtttga ttttacttgc attggcatta ggtgggttac
     1021 tgatcaaata tctgatcgtt gataccatcg ttcaagaatt aaaagaaaag atggaccgac
     1081 cttctcgttt agtcgggttt actgccttga gctgtatcgg aatcaatctg atcgttggag
     1141 aacagtatct gtccatcatt cttccaggag aaacattcaa gcgttctttt gatgacgttg
     1201 gcttatcaaa aacctatttg acacgaacac tagcggattc aggtgcgacc gtcaattctc
     1261 tcgtaccgtg gggagtcagc ggcacattca ttatgggaac gatgaaagtt agcgcgttgc
     1321 attacttgcc attcgccttt ttctcgattc ttgcacctgt ctttacaata ttaggcggtt
     1381 ttttattgaa tcgtcgacgt gtaaaaaatt gaaggaagga aaaaagaggc tgggacagaa
     1441 gcgtttaact ccaagaaata agaagaaact caagaaaatt gctttttaaa tttttgtgaa
     1501 tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg tttagagcgt
     1561 gagacaaaag tgttctttac ttttgtccca cgctcttttt tacagataaa caatgtgtta
     1621 gaagtagtat cttcttttac ataaaaagct gaccatagga taaatggtca gcgtatttag
     1681 tgagtcagct atttagtatg ggttttctaa aggaacgact gtctctcttt tgatcaagcg
     1741 atgagggatg accaaacgta cgccactgct caattcttca tggatcaatt cgcgcagctt
     1801 ttgcatagcg acacgtccta attcggagat atcgatatcg atacttgtaa ggtaagggtg
     1861 tgttaatgtt gagaagatcg agttgttgaa gctaatgacg gagagtgtct ctggaatgac
     1921 atagccatac atttgcgcca actgcatcgt gcgaagggcg aagatatcgt caatgaccac
     1981 caatgctgtg gcttcgtttt ctttcaacgt tttctcaaag gacagataat cctccggatg
     2041 ctctagtact acactggata acgcaggaag accagccatc atcattgctt tttgataacc
     2101 gaaatatcgt tcgaaataca aactttcatg agtcgtgttt gtcacaaaca ggatattctt
     2161 atgtccattc tggatcaaaa attctgttgc ttgtttacct agaagctggt tatcattatc
     2221 tacataaacg atttcgttct catgatgata aggctgtccg atcaaagtga aagggatgtg
     2281 gttttggtat aaataatcaa tgacagtatc ttgactatct gaatacacta gaataaaacc
     2341 atctacttgt ttttgtaaat gcatacgctg gacgttctct aacagcaatt caaaattttt
     2401 agcagtagcg attgctgtag tcatatcata attccgcgct tcttcgttga ttgcttccat
     2461 gatctcaaga taaaatgggt taccgatccg ctcttttgaa tcaagagggg gtaagattac
     2521 gccgatggca ctggaaatgc gttttcctag attgcgggca gtcatgttag gaacataacc
     2581 tagttcatcc ataatttttc ggactttctt ctttgttgtc tcggaaatac taggatgatc
     2641 gttgatgaca cgagagacgg tcgaggtggc aacaccggct tttttcgcca catcttttac
     2701 ggtaatagcc atgataaaaa cacctttctc ttgttattgt gcagagaacc gataaatcgt
     2761 ctgactgatc cgttcttgac caggatggag gttgatcgat ccaaatttgg ggaaatgaac
     2821 gatatcaggg atttcttgcg gttcgattgc taatccgtag tttgaatgca tctgttttcc
     2881 attgacagag aaatcagctt cgaatccagt cgtagaaaat aataccatac ttttattaga
     2941 agttgctata gtcatttggc gaccagaatg cttctcgaat aattgtaagg aagttttgga
     3001 aagcctagga tgatgcaaaa gaaatacatc atctagtcca tctggataac atgcaaggat
     3061 ctcttgcagt gttggggatt ttctaaagtc gattggcagt ttttcttttt ttgtccaatt
     3121 tccagtaggc agtttttctt gatctaattc aagcacacca tctaaaaaga ccgttaactg
     3181 atgttcatag atgtctcttg ttccatttcc tgaaaggttg aagtaggcat ggttggtcgg
     3241 attgcagatg gtttcaacaa ggctactagc agaggttgtc atctctaatt cgttttttgt
     3301 taaacggtaa gtgatcgtag cagtgattgg tcctggatag gtagaaaatt catcttttaa
     3361 gtaaaagacc acacctattg atgatggagt tttgaaaact tctacttccc aaaattggaa
     3421 agaccagcct ttcgtgccac cgtggatatg gtgtgatccg cagtttttct ccaatctatg
     3481 ctcaccccaa gaagcatccc ggatccttcc ggctacaggt ccgacagttg ctccaaaaaa
     3541 gcttttgtct gttagaacat cttcgaaacg atcgtaagac aataaaacat tttctgattt
     3601 tccttctttg tctggtgcga agatttggtg caggcgggca ccgtaattga gaagaacgac
     3661 ttctaagtag tcgttcttca atgtgatttt ctttatttca gtctctggaa ctggtacgac
     3721 ggagatctct ggattatcca tggtcatgcc acttttcttt caagtaatcg atcgtaagaa
     3781 aagtcgtatc agggacaagc gggatctcct ctccaaggtc ttcagcacgt cccacgccaa
     3841 ttggttgagc gccacttgag aggatggctt ggataccggc ttgcgcgtct tcgatcccga
     3901 tacattcttc tgcttttagt ccgacagctt ttgctgcaag gaggaagata tctggcgcag
     3961 gttttccgtt tgttgcttct gccgggtcgg caatagcatc aaagtaaggt gtaagaccca
     4021 ttttttcaag taggaatggc ccgtttttac ttgcagaagc aagagcaatc ttgatttttt
     4081 cttcttttaa agaatccagt aaaggcacga ccccggggaa gacatctttt ggttcgatcg
     4141 tctggatcat ctctaaataa taatcgtttt ttcttttcgc taattctgca aattcttcct
     4201 ttgaaaaaga gtgttctttt ccgccataag ctaaaagcaa agagagtgag tcttctcggc
     4261 tgacgccttt tagctgttcg ttgaatgtgt ggtcgatcgt aatgccgatt tcctgaccaa
     4321 gctttcgcca agcacgataa tggaattcag cagtatcggt aatgacaccg tctaaatcaa
     4381 acaatacccc tttaaacatt tgcttcctcc tttcttagcg ttatttctag acgatccgct
     4441 aaagaataag actgttcata gacgtaaatt tctagaggag aaccttctaa taactcgaaa
     4501 gtaacttgtt catctgtgac tgagatgaac aataaacgtc cgcgataatt gatatggaaa
     4561 gaataagctt tccaagtttt tggcagaaat ggtgcgaatc tcaactgatc atgggcagtt
     4621 ttcatttgag caaatccttg gacaatcgac aaccagctac cagtcatgga agtgatgtgc
     4681 aagccgtctt ctgtgtcgtt attgtaatta tccaaatcca agcgagctgt tcgctggtac
     4741 atttctacag ctttgctttc cattcctaat tctgctgcta aaatcgaatg gatacttggc
     4801 gaaagggaag actcatggac agtcattggt tcatagaaat caaagttttt ttgcttttct
     4861 tcttttgtaa aagtatcatt gaagaaataa atcccctgta gtacatctgc ctgcttgatg
     4921 aagcaagatc taaggatttt gtcccaagac caatgctgat tcagaggaag atcgcttgtg
     4981 ggaatatcgg atacaggcat gagatcttta tctaagaatg tgtcatgctg gacaaagatt
     5041 cctaattctt cgtcaactgg gaagtacatg ttctctgcga tatctttcca tttggctagt
     5101 tcttcttcgc tgatagtgat agcagcttct ttttgataag ttaaataatt ttctcgcgtg
     5161 taacgcaaga cccaagctgc gatcgtattt gtataccaat tgttattgat attgttctca
     5221 tattcgtttg ggccggtcac accgtggatc atgtatttat ttgctcgttt tgaaaaatgg
     5281 acacggtctg cccagaagcg tgcgatttct gctaaaactt ccagtccttc atttttcaaa
     5341 tattccgtat cgccggtgta attcgtataa ttgtagattg cataggcaat cgcgccattt
     5401 cggtgaatct cctcgaatgt gatttcccac tcgttatggc attcgacacc agtgaaggtg
     5461 accataggat aaagcgcgcc ttttaatcct tgttgctgag cgttgtggat tgcttgcggc
     5521 agttgattat gccgatattt cagcagattc ttagttactt caggtttggc taaagcaaga
     5581 taaagaggga cagcatacgc ttctgtatcc cagtaagtgg ctccgccgta tttttctcct
     5641 gtgaatcctt tggggccgat attcaatcgt tcatcttcgc cgtagtaagt agagaatagt
     5701 tggaacaaat tgaatcgaat accttgttgt gcttcttcgt ctccttcgat gaccacatcg
     5761 gcaagcttcc atcgttctgc ccaagccgct gtttgctctt cttttgcttg gatataattg
     5821 tttgtctggt tcgttaattc tgagacagct ttttctgctt gctgcgcttc tggaatatct
     5881 ctactagtaa gaacgataat ttctttttcc actgaccatg tctcaccttg agataattct
     5941 gcagacaagc aaccatgtaa tgctaaagta tcttcgtgat agtcagggat gactgatttt
     6001 ccattaacga aatggcgcat tgctgcagta acagtaaatt gttcaatccc aaagttgttt
     6061 ggaatagttt tagtcgtcaa atagccgatt tcttctatag ttccacgatc aatctcattc
     6121 caaaaatgtt catcatagtt gctgtcttcg ttttgaacat ttccatccaa ttttgatacg
     6181 agtttgactg ttccgctacc ttcaagcatt tctatagtta aatagatata agctgattct
     6241 tttttgacaa tgctcagaaa acgttcgaag gaaatgcgta ctttattagt agaagtagtg
     6301 attgtgaatg tacgagataa tacaccattt ttcatatcta attcccagta aaagtcttct
     6361 ggttccaaag aagccaagtc gatcagctga tcgttgatcg aaatatctat cgcgataaag
     6421 ttcaaggcgt tgatcacttt tccaaagtat tctggataac catttttcca ccagccgaca
     6481 cgtgtcttat cgggatacca gacacccgcc aaataagtac cttgatggtg gtcccctgag
     6541 taagtttctt caaagttccc gcgcatcccc atataaccgt ttccaatact tgttaaagat
     6601 tcttgcagtc gaagattttt tttatctaat tgtgtcgtgc tgattttcca tggatcaata
     6661 gcaaatagtc gtttgatctg tttcattttt ctcctcctaa tgagctgttt tatacgttca
     6721 taataaacgc aaccgattgc acaagtcaat agacaaaaga aaatatttca tagaaaaaag
     6781 cttaaaaaaa taaattttta ggagaaaatt atagaaggga acacacgtat tataggtttt
     6841 atagaatggt atagacagaa aaataaaaaa taaagcattt acaaaaaaga aaacgcacgc
     6901 tataatacgt ttaacgaaac cgattgcgta aaggagctaa acggaatgaa aaagatattt
     6961 agttttgagt tttggcaaaa atttggaaag gctttaatgg ttgtggtagc agttatgccg
     7021 gcagctgggt tgatgatcag tatagggaaa tcaattccct tgatcaatcc tgattggacg
     7081 ccgcttgtca caacgggcgg agtaattgaa aatattggtt gggcaattat tggtaatctt
     7141 catattttat ttgctttagc aataggagga agttgggcaa aagaaagagc aggcggagca
     7201 tttgcagctg ggatctcatt tattttaatc aatcggatca ctggggctat ctttggtgtc
     7261 acaagtgaca tgcttgcgaa tgaagatgca tttacccaca ctctgttcgg tacgaagatc
     7321 atggttaaag gattttttac aagtgtattg gaagcaccag ctttgaatat gggagtattc
     7381 gtagggatca tcgctggatt tgtcggggca atggcttata ataagtacta taattataga
     7441 aaattgccgg atgctttgtc atttttcaat ggaaagcgct ttgttccgtt tgttgttatc
     7501 ttatggtcaa caatcgtggc attgattctt gctgtggtgt ggccaaacgt acaggctgga
     7561 atcaataatt ttggtttatg gattgcagaa tcacaagaaa gcgcaccgat tctagcaccg
     7621 tttttatttg gaacattaga acgtttacta ttgccatttg gattgcatca tatgctgacg
     7681 attcctatca actatacaca gcttggcgga tcgtatgaga ttttatcagg cgcacaggcg
     7741 gggacacaag tatttgggca agatccgtta tggttggctt gggccactga tttagtgaat
     7801 cttaaaggtg caggggatat gtcgcaatat gagtttgtgt tgcataactg gacgccggct
     7861 cgtttcaaag taggacaaat gattggttct tcaggtatct tgatgggaat ggcttttgcg
     7921 atgtatcgta atgtcgatcc tgataagaaa gcaagatata aatcaatgta tttttcagca
     7981 gcgttggctg tctttctaac aggtgtaaca gaaccattgg agttcatgtt tatgtttgct
     8041 gctgtgccat tatatgtcat ttattcagtg atccaagggg ctgcatttgc gatggcagat
     8101 atcttgccat tgcgggtcca ttcatttggg aatatcgaat tgctgactcg gacaccgtta
     8161 gctatcaaag cgggtctagg tggagatctt atcaacttcg tgttgtgtgt gatcgctttt
     8221 ggtgttgtta cgtatttctt agcaaatttc ttgatcaaaa aattcaactt tgctacaccg
     8281 ggacgtaacg gaaattacga caatgacagt gaagaaaccg ttgtatctaa cagcggaaca
     8341 ggaacagcag atcagcaagt ggtccagatc attcatttgc tgggaggaaa agaaaatatc
     8401 aaagatgtcg atgcttgtat gactcggtta cgcgtaagtg taaatgatcg agaaaaagta
     8461 gggactgaag aagcctggaa acgagcaggg gcgatgggat tgatcgtcaa agacaatgga
     8521 gtccaagcag tttatggacc aaaagcagat gtcttgaaat cagatatcga agatctgctg
     8581 caatcaggag cagagattcc agagccagaa atggtttcta caggaaatgg acaagcaggt
     8641 agtaagcaat acttaggtat agaacaagaa ctagtttccg cagcttctgg agaagtgatt
     8701 cctttatctg aagtaaaaga tccggtattc tcacaaaaaa tgatgggtga cggctttgct
     8761 gttattccaa catcaagaga agttgttgcg ccgatcgctg gaaaagtgac aagtattttc
     8821 cctagcaaac atgcaattgg gctggaaacg aaagatggta tcgaagtttt gatccatatg
     8881 ggaatcgata cagtccaaat gaatcagcca gcttttgaga ttgctgtcaa agaaggccaa
     8941 gaagtaggaa caggaacgaa attagctgag atggatcttg atgtgatcca aaacgaagaa
     9001 aaagatccaa cgatcatgat cgtatttaca aacgataaag tagaagaagt agtgatcaag
     9061 caattaggta cagtgacagc tggtaaagtg atcggctcca ttaaaatgta aggagaggtt
     9121 gtgaaaaagc tgtcctgcat caagtcataa gaacagccaa acaaaaatag ctcctcctat
     9181 ttttcgtttg gttgggctta ttaccgagaa gcagcagttt gaatactgtt tagtggagag
     9241 gtcgtgaaaa agctgccctg caccaagtga taaaaacagc caaacaaaaa atagctcctg
     9301 gtccattgag cttattactg aggagttagc aggagaacac tatttgctgg agaggttgtg
     9361 acaaaaattt tgtcacaacc cttttgtttc gaaatgaaga caaggagaaa accaatgaaa
     9421 ttcatgacac tcaacacgca tagctggctt gagccagaac cagagaaaaa attgcaagaa
     9481 ttagcagata agatcctgtt agaagattat gaagtgattg ctttacaaga aatcaaccaa
     9541 ttactggaat ctgaagaagt agaacctgcg aagctaatga agttctgtcc ggtaaagaat
     9601 caagtaccta tccgtaaaga taattttgcc taccgtttag ttcagctttt aaaggaacac
     9661 ggaaaagagt atttttggag ttgggaaatg agccacatcg gttatgataa atatgaagaa
     9721 ggaaatgcac tgcttacgaa aaaaacattg gaaagtcagg tccttaccgt ctcccaattt
     9781 caaaagaaag aagattatca gacacgcaag atcttgattg ggaaaacaaa gatagatgat
     9841 caggacattt ttgtctcgag ctgccacttt tcttggtgga cagataaaaa aagtggcttt
     9901 tattttgagt ggaaaaactt ggaaaactac tttttggaaa ctcgcgtacc actttttttc
     9961 ctaggtgatt tcaataatcc agtagactct caaggttatt atactgtaag agaaagttgc
    10021 cttcttcttc aagattcgta tgttgtcgcg aatgaaaaag gaaaagcagc tactgtggaa
    10081 aagaaaatag atggttggga acaaaataca gaaaaattgc gtatagattt tattttcgtc
    10141 cctgaaggaa tgcaggttaa aaaatatcaa cggatttttg atgggatcga tagtccaatc
    10201 atcagtgatc actacggggt ggaaatcgaa ttagatgtga acgaataaaa aatataacat
    10261 ataacataaa aaaactgatt aaaataaaac tctccaaacg attataccgt ttggagagtt
    10321 ttttgatcgg atgtatttat cccttttgcc taagcacgta aaagcttgta tctatgataa
    10381 ataaataaac atagcaaggt aaaccagcca atcaaagtaa gagaaagaat gaaatagaaa
    10441 tagattggcg tttgataatg aagcgtgagc tcgttgattc ctttttgctg cttcacgatt
    10501 ggggtcccga tatctgttac ttcataatca gtcaatgttt ttccattttg ctgtagcaca
    10561 gtccgatcgt agacgataac agggatatga acagttttgt ccccagtgtt tttccacttg
    10621 attgtcaaaa gaccgcctgg atgtgttttt ataaaagttg tttcgttttc tagtatttct
    10681 tcgttatacc ggtcgtattt attgtctttt gtctccttat agattggcag atagtcaggt
    10741 gtggtttttt ggaaaacgaa caagctggta gacatatctt tgctgaagaa tgttttcctt
    10801 gcttcttccg gcgtatcgaa tagataagta tgtcgactga caaatgtttc attttcccac
    10861 ttttccagat gctggtataa gttctggatc gtctgtgctg aaaaaataag aatgccaaat
    10921 atacttaaaa gacggaacca cttttgttca gaccactggt tcagtgaaag ggcaaggtaa
    10981 agtagaagca atagcgtaaa tggcacgaaa aatcgaaatg gaaactggat caaactaata
    11041 aaaggcacat ttttttcgat caatgtcgtc catggaatca agctagtaga aagaaaaaag
    11101 aaaacaagac ttgtgtacaa cacgattttt tggaataagg tgttcttttg ttgaaagaaa
    11161 ataaaaagca aaaaacctga taatatcaat ataggaaagg ccttagggta atggagccaa
    11221 taactgccat ccagatctat cgccttttga ctcatttctc tgttaacaaa gggaggaaga
    11281 atcttattcc cactgtaaac aatagctaat cctgcccaaa tatttgctgt cagaacaaaa
    11341 taaagcaaga ccgacttaat cagttgaagc agtgcttttc ctttttcagt ctggttaaaa
    11401 aagtaataaa gataaaaagg gatatacatc aaagctaaca tgatcgaaga cagtaaatgg
    11461 atctgtgtca taagtcccac tgagacagcg actttgataa cagggaactt tttatgaagg
    11521 acgaaatcag caatagggat catacataaa ggataaaaac aagtccccca gctagtgaat
    11581 ccttggttga tagaccagta ttgaatagaa tacgtactca tgaataaaac ggctactggt
    11641 acacttaccc aatttcgaac attgacatat ctcagtaaaa tcagcataga aaatcctgaa
    11701 ataagataaa gaatgaaatt tgaaagaatc tgataatgaa accagctacc tgcaagcaac
    11761 acgatcaatc cttgtaaata agcgaaaaat gggccgtaaa cggcattcac gatcctgcca
    11821 ctttgctgga atccataaag ggaaggaaaa taatggaaat ccccatattt aatttgttgc
    11881 gcggcatcat agaaccgatt gtaatggaat ggggcatcag cacccaaaat tagtttatgt
    11941 gtgaataaat gaggcatgag aaatgccaaa gcaatgaaaa aaattgctaa aaatgggcca
    12001 tattttttca attgtcgaac tcctttgcaa actttagata aattcatcat atatcctatg
    12061 cgagaggtag gcaagaaatt agaaaattca tgaaaatatt tatgaatttt acaagaaaaa
    12121 cataagtgaa agttctaagg agagaggatg cgacataagt tttgttgcat ctccttgttt
    12181 tgaaacagaa tttttcttaa tactcgaaac aaagtagctt tttcatgacc tctcggtaaa
    12241 cggtgttcaa tcagctgctt ctcggtaata agctcactag cctagaaaat atgagaagct
    12301 attttctgac tagcgctctt attactcaaa gcataacgca gttttcacaa cctcttcctt
    12361 aaacggtgtt caaaagcttg catctgcggt aataagccca acccaacaaa aaatatgagt
    12421 agctattttt ttgggctgta cttattactt gatgcaggac agctttttca caacctcatc
    12481 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
    12541 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
    12601 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
    12661 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
    12721 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
    12781 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
    12841 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
    12901 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
    12961 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
    13021 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
    13081 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
    13141 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
    13201 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
    13261 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
    13321 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
    13381 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
    13441 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
    13501 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
    13561 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
    13621 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
    13681 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
    13741 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
    13801 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
    13861 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
    13921 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
    13981 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
    14041 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
    14101 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
    14161 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
    14221 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
    14281 ctttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
    14341 tgaggaacta tttttccatt tgtttctcta atactcaggt caaaacgctg atttcacaac
    14401 ctcttgataa acggtgttca aacggctgct tctcggtaat aagcccacta gcctagaaaa
    14461 tatgaaaagc tattttctaa ctagcgttct tattactcaa agcataacgc tgatttcaca
    14521 acctcttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
    14581 acatgaggaa ctatttttcc gtttctttct ctaatactca ggtcagaacg ctgatttcac
    14641 aacctctctt tcacaacctt attttactcg tttataattt tcatcgaagt attttccgct
    14701 acggatatca tctggcattc catcgtatgg ttcttcgcta ataacatctt cattgtctat
    14761 acctgcatca cccatataag tgatcgtagc aaattcagga acaagtaatt ttggataagt
    14821 ttcatatttt tcacgagctg cttccatttc tgcgatatgt tcgttttgga aagtaacagt
    14881 gatttctgga tgttcttcaa cccatttagg gaaaaagatc attccgtgaa gtttcttttc
    14941 atttggcata aagtccaatg ccacgtctgt ccctgttggc tctgtccaag tgattttgaa
    15001 tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct tttacccaac ggcctttgac
    15061 cattccgcca tggatacggt aatcaacagt atggtcattt ttggcatacc attcatattc
    15121 ccaaccattg tcatacgtat agataaagtg agttccaata aaatcttcca attctttaaa
    15181 ttctttcatg tttaagcatc tccttataaa ttaaaacatg taataaacta catgttttcg
    15241 attactcatt tatgatagaa tggatttaaa gatttgtcaa ggagggaaaa ctgatggcaa
    15301 gaaaaaatac acttcgatat attttgttag ggttattaag taagaagaag atgacaggat
    15361 atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt ttggcaagca aaacatagtc
    15421 agatttatcc tgaacttgca aaaatggaag agcaaggaat cattcagcat caagtagaga
    15481 tcacaggtga aaaattggaa aagaaagttt atgaattaac agaagaaggg aaggaacaat
    15541 tggcagagtg gattcataca cccaccaatg aactaccggt taatcgagac gaatttgttc
    15601 tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt agtagaaatt attcagcagc
    15661 agcgtgacct tcatgaagaa aaacgacttc atctattaga gcgaaaaaaa acgatctttc
    15721 ctactgaaaa agaaaaacat gaaaattacg gacactacct gattttagcg catgcgatca
    15781 atcgggaaac agaatatgca aattggctaa cagatgtatt agaagaaagt aaccgaaaaa
    15841 aataaaaaaa tatccgaaca gtgatggaga attcctttct atatgaaaga aacgcagatc
    15901 accgttcgga tattcttatg ttcaaacagg ccttttttca agtatttatg tagcatctac
    15961 ttattaaaaa gcttggtact taggagatta gtggcgttta gagttctttt aacaactgtt
    16021 cgtagtcagc tttttcatcg acaggaagga gcttgtaagt taattcgtct gtctcgttgt
    16081 catgaatgat gtacatctta gcattctctt ctttgaagtt accgatttgt aaaatcgtat
    16141 agcgttcgtc gatcaattcg atatctttga ttgcttttga aaaaacttta cggacatggt
    16201 gcattttctt ttggcggatt tctgcctttt ctgcagcttt tgacaaagta aatccgtcat
    16261 ctaaatattt tttgatcagt tctactttta tgactgtagg taaacgatat tttctaggac
    16321 aattcgcatc agcttcgatc gaacgaatga atcccttttg ttcccaatac cttaattgac
    16381 gtggagacac acctacgata tcacttaatt cactgatgcc aacaaccaat tgatcatttt
    16441 ccaacaaacg tttaagggaa gccccaacca tttttgtcac tccttttgtc ctcttgcttc
    16501 ccttaattat atccgtatta acttatgtgt caagtaggtt gacaaatagc gaatttagga
    16561 ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa ggagaaatat gagtaacaac
    16621 caacccgtag atatttacgg aaaaccatat aatcggacac ttttagttgt tgtcttatta
    16681 attggtacat tttgtaccgt tttgaatcag accttattga cgacagcttt tcctacattg
    16741 atgaaagctt tcgatatttc agcgtctgat gtgcaatggt taacgactgg tttcttgcta
    16801 gtcaacggga tcatgatccc aataacagca tggctgatca ataaattcag ttcaagaaat
    16861 ttatatcttt ctgccatgac gatctttttg atcggaacga ttacttgctt tacagcacct
    16921 aattttagta cattactaat cggtcgtttg atccaagctt gcggtgtggg agtatcgatg
    16981 ccattattgc aaaatatcat gctatctatt ttcccgccag aaaaaagagg atcagcaatg
    17041 gggatgtcag ggatcgttat tggacttgct cctgctttag gccctacatt atctggttat
    17101 atcattgata actaccattg gcgtgacttg tttggtatgg tactgccaat cgttgttctt
    17161 gtcttggttt tagcattctt cctaatgaaa agtgttattc cattgtcaaa tcctagcatt
    17221 gatattttat ccgctatttt atcaacgatt ggattcggta gtttacttta cggattttct
    17281 agtgtaggag atgacggctg gggaagcttg aaagtaattg gtttcttagt tgttggtgtg
    17341 attttcatcg ccttgttctc atggcgtcaa ttacatttgg aacatccatt cttggaactt
    17401 cgtgtattca aatcatctac attcacgatt gcagctgttt tagctggtgt aacaaatatg
    17461 gcgatggtag gggcagaaat ggtcgtacca ttgtatattc aaaatattcg tggagaatca
    17521 gcattccatt ctggcttgat gttgttgccg ggtgcattga tcatgggggc tatgatgcct
    17581 gtgacgggtg cgattttcga taaatacggc gcgaaacgtt tagccatcac agggatgttc
    17641 attttgacag cggcaactgc accatttgct ttcttgacaa aaacgactcc agttttatat
    17701 atcgtgatcc tttatgcgat ccgtatgttt gggatctcca tggtcatgat gccggtaacg
    17761 acttctggta tgaatgcatt gccaaataac ttgatcagtc atgggacagc ggtaaataat
    17821 acattccgtc aaatcgcaag ctcgattggt acagctattt tgatcagtgt gttgacgaat
    17881 gtgacaaaag caaatatccc aagcaaatct gtattggata atacgccatt agcttataaa
    17941 gatcaggcaa tcaatgccac actgtcaggt taccatgccg cgtttatcgt agcgatcatc
    18001 tttgggattc ttggctttat tattgccttt ttattgaaga aaaaagaacc tgttctaatg
    18061 aaagcggggg ataatgcatg attattggag ttttgattgt cagcttgctt gcttttgctt
    18121 taacgaacgt gttcgctaaa aaaacatggc aaaccttttt atctttgatt ttcggactaa
    18181 tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc caatcacttt ggaatggaaa
    18241 aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc tgattcccaa ggagcagata
    18301 tgcttttgta taaagcttta gggaatggaa aagaaaaagt gtatctttac cgaacaaatg
    18361 aaaaacaaga aaaaccaaaa gcaactggta cggataacga aacaaataaa gtagaaaaga
    18421 cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg ggaatacaaa aacgacatgt
    18481 ataaattctg gttcaatatt gctgataaca atcacgaata tgacagccga gtgaatacat
    18541 tcaaaatccc agagacatgg gtagaattaa gtacggacca agcagccaag ttagctgaat
    18601 tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa agcttatgtt caagacggta
    18661 tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc agagcaagaa caacgtacga
    18721 aagccttagc tgctgaattc caacaacaag cattcgcaaa attagtgaaa gaagcaaaag
    18781 gcgaataata ttttgaacac catgta
//
LOCUS       DASJST010000048        18676 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJST010000048 DASJST010000000
VERSION     DASJST010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.048"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(83..481)
                     /locus_tag="U4B05_001343"
     CDS             complement(83..481)
                     /locus_tag="U4B05_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN2050494.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(673..1440)
                     /locus_tag="U4B05_001344"
     CDS             complement(673..1440)
                     /locus_tag="U4B05_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2050495.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(1475..2368)
                     /locus_tag="U4B05_001345"
     CDS             complement(1475..2368)
                     /locus_tag="U4B05_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN2050496.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELI
                     KKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKL
                     LIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIK
                     NTVKYLEKSITSYLPYVNLYD"
     gene            3349..4152
                     /locus_tag="U4B05_001346"
     CDS             3349..4152
                     /locus_tag="U4B05_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2050497.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            4737..5192
                     /locus_tag="U4B05_001347"
     CDS             4737..5192
                     /locus_tag="U4B05_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050498.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            5311..6369
                     /locus_tag="U4B05_001348"
     CDS             5311..6369
                     /locus_tag="U4B05_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEN2050499.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            6411..8360
                     /locus_tag="U4B05_001349"
     CDS             6411..8360
                     /locus_tag="U4B05_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN2050500.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            8363..12004
                     /locus_tag="U4B05_001350"
     CDS             8363..12004
                     /locus_tag="U4B05_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050501.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            12404..12742
                     /locus_tag="U4B05_001351"
     CDS             12404..12742
                     /locus_tag="U4B05_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050502.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            12852..13073
                     /locus_tag="U4B05_001352"
     CDS             12852..13073
                     /locus_tag="U4B05_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050503.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            13093..15249
                     /locus_tag="U4B05_001353"
     CDS             13093..15249
                     /locus_tag="U4B05_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050504.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            15261..15494
                     /locus_tag="U4B05_001354"
     CDS             15261..15494
                     /locus_tag="U4B05_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2050505.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            15484..16350
                     /locus_tag="U4B05_001355"
     CDS             15484..16350
                     /locus_tag="U4B05_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050506.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            16365..18335
                     /locus_tag="U4B05_001356"
     CDS             16365..18335
                     /locus_tag="U4B05_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050507.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            18502..>18676
                     /locus_tag="U4B05_001357"
     CDS             18502..>18676
                     /locus_tag="U4B05_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050508.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         6769 a         2713 c         3573 g         5621 t
ORIGIN      
        1 atattctttg ataatttgtc tttcttaaat ccatacgtcc taaaacaagc tgtttttgct
       61 taatatgatc atctgagaga catcaaatca acttttctct caacaaatca ttcaaagctt
      121 tcgacagatt aatattctct tcagccgcta aaacatttaa atagtgttcc acagtcactg
      181 attttcgatc ataattttgc ttgtttcgag ctttagcaac tttcgtatta gcttttacga
      241 caaataaatt aacattctca gatcgcttga ttcttgcatt taactcttga acaggcgtag
      301 gcgatggaat ttctttttct tgcaactcta aaagcaccaa gtatccttcc aatacttctt
      361 gcgccatctc aaaagcatct tctaaagttt caccatgtgt atataagtct ggaaaatctg
      421 ggaatttaac tgcatattca tttcctttcc attcgaatac tgcacaaaaa aagtatttca
      481 tcgcattgca cctctctaat taaaaaataa tttttgcttt tttgtttcaa aaaaaagaat
      541 cgcctaccaa aaaaattgat aggcgatcac aagctaatta acctctaaag gagattaata
      601 ccttgctccc aaggcactat atatcttagc atacgtctga agcatcttca aacgagtaag
      661 atatattttg cattaaccta attttttttc tctataagtt ttttcatcaa actcactaga
      721 aatattaaac tggacagcaa aattgtaatt tctgcctttt ctaactttag caaaatcaat
      781 attcagcatt gatttttcat taatttcctc aactgattcc cttaaaacac gtctatcaaa
      841 attcgatatt gttttatact cattttctgt tttcgtaatc tcacgaattt taactatttc
      901 aatcagggga ttcttatata tttcagcttt ctcaaaatct cctttatttt tggcgataag
      961 atatttttta taatacatcg ccaaatattt gtataacacg atgctatatt ttttttgaaa
     1021 agatgctaag tcttcaaaaa caaagaacgg atacttcttt ttgattgaga caattaaggg
     1081 aattacatct tctgaaaaac tgaaagatac actatgctct tctaaatccc aatgaatccg
     1141 atcaatagca gatacagtag tatacgtctt atattttttg tctagattcg aaatttcata
     1201 aactgcttgt ctttgcaaag tgttaactat ggtttttagt tccgtttcaa gattttgatc
     1261 aacatccatg taattaataa tcgctttaac aggtaaatct actacgcctg ttttctttag
     1321 ctttgtgggt gagatataac tgaacagcaa ttgataaagt tcatacgact ttaaatccat
     1381 tttcatgtca attgttgcta agaactcctc attgtacttt gtcagttcat cttttttcaa
     1441 aagtttttcc ttccttcacg cattttttca gttgttaatc ataaaggttc acataaggca
     1501 aataactcgt aattgatttt tcgagatatt taacagtatt ttttattgtg taatcctcct
     1561 tatgagtagc aacgtaagat aggtgattga caactgattc aaatcctcta tctttggata
     1621 aacgatcata aagcggataa actttattct gaagtcggct cataagttta agatcgcata
     1681 agtcggcaga actgatcaaa aagtttttga ttagtaattt agtgtatttg ctattcatag
     1741 ctttcaagta cgattgattc attttctctg ttttatcttc tgcgcctttt aaataagcag
     1801 ggtctctttg ttcttgctta taaggtaccg gaactgacat ggtacgtgta agcaagaact
     1861 ttatgtttgt ataagtatga cccttcttga taagctcata gtcaatttta aaatcagtgt
     1921 atttattcag ttgggcaatc ggttctttaa ttacacgtct ttcaaagtct cgaaaagctt
     1981 tatatttttt ctcaatacta aatttctttc gtaaatctgc aatggagaaa acaggatttt
     2041 ttaatcttga atgttttgaa gaagatgaac tagaagacat caacgcaaat aaagcctttt
     2101 tagatacttt gacggtatta ttaaaaggcg gatttttagg gtcaagatgt cctatagcgt
     2161 aaagataaat tttttgtggc atagcatcca tttcaacaat gctatctaca agttgattcg
     2221 ataaagtaac agttaaatca aactttttta gattgctttc ggtagtttca aatttcgggt
     2281 ttacattctt ttctgttatt ttcatacgat tgcctcttct ttctaagctt ggtacgagct
     2341 taaaaacttg gaactggtac gattccattg aataagtata gacctgtttt cgatacgtta
     2401 caataagaaa tgtatcgtaa atttataaca taactcaatg tcagtaaaac acattttatt
     2461 ttttccaatc aaatttcatt gaaaatacag cagaaatcgc tattttagaa cattttcaaa
     2521 ataaaaatcc ttttcaataa aatacgaaat tataaaaatt accaaaatta aaaaacaaat
     2581 atttttctag tttttagaaa taaattcaca tactttttct ttttttggat atattcggaa
     2641 taccacatcg ctttttgtcc acgtttgcat cgctttttgt ccacattcac atcgcttttt
     2701 gtccatgttc gcatcgcttt atgtccaccc taaaatctat ttaagattga tataaagcct
     2761 ttttggaggt tgctaaagtt ttaaagatta aaatatatat tataaatata aataaaaggg
     2821 gaagctgaag cttcctcctt ttcctttttt tcaattattc aaagttacac aagcaaaata
     2881 aactcctaga tagtgttagt tttttttaag attttttatt tttaagattt tgaaataaga
     2941 gaggagtaat cctcctttat taaatatctg ttaatatatc tgttaaatat ctgttaatat
     3001 atctgttagg ggaggcttaa tctcttggga gagtaagtat taaaggggta taaaggtaac
     3061 acttttggac agtaaaggta acaaaagtgg acagtaaggg taacagtttt ggacagcaaa
     3121 ggtaacaaaa gtggacattt gaggtatcac actaggataa gacgttacct tcggttttct
     3181 tgatttggaa ggcgaaaaaa tagaaaatta gattagtaac aaatctttgt tgactgttac
     3241 ctttagttgt gatttaattt aggtaacaag cagaaaaaat tgtttccttt aattagtacc
     3301 agattaacat gattaaagga gcgcaaaaag aagattggag agtaatcaat gtctcaagaa
     3361 gttaccgaaa agattattga tagtgatgta gatttagtat cagaacaaac aaatgcaatt
     3421 gtgcgttata acaatggtct gaatacaaca gctttgcgag gatttaagcc tgttgaaatg
     3481 gatgtgtttt gggctatttt atcaaaaatg aaacgtcaag gaacagcaga agtaactttt
     3541 gatttcaacc tctttcgtta tttggtcaaa tacgacagaa gagaaaagga cagtttttat
     3601 tctgtgttaa aagatttgag tgacaaacta ggtaatttaa cctaccgttt tgaagattca
     3661 gacgtctatg aacagctgtg gctttttcaa aaattccgga tagataaaca gaaggaaaca
     3721 gttacaatta aggcaaatga ggaatttgaa tttatcttaa actcaattgg caaaaacttt
     3781 actcgttttg aacttgataa ttttacaaaa ctaagaagta gctacacaaa agaattgtat
     3841 cgtcatctca tgcaatacag aaataaagag tggcgaaatg gttattgggt agtcaaagtt
     3901 gaagatttta gaagagcatt aagtatacct tcaagttata gaatgacaaa tattgataaa
     3961 agaatactag agcaggcgaa agaagaattt ttagctcctg atgaaaacgg aatacaggta
     4021 tttgaaactt ttgattatga aaaaatctat gcaaagaaag gaagaagagt tgatcggctc
     4081 agattcactt ttagtgagcc agaatcaaac ttgccaacca tttcaatgca taattggtta
     4141 gaagaagatt gagaagaagc aggttgtaat tttaatacaa cctgcttctt taaaaaagta
     4201 tgggtaacac ttttggacaa taaatgcaag aagttttttt gtaagcaagt ttcagtctaa
     4261 ataatatttg aaagaatggc taatctatac aaattttaac aactatgggt aacacttttg
     4321 gacaagtcaa gaaaaaaaac aatttttctt aatttgttga tcgactaaag gtaacacttt
     4381 tggacaacac agaataaaaa gtccggtgat atcagttttg gtaggaaaca tcttaatatc
     4441 tttgtgagat tgaaggtaac atttttggac aagtcaacta tttgttaaaa gttaatatag
     4501 agtaattaga taagtttttt cttttcgtct gataaatgga taatattagt tattttattg
     4561 ctaattagtc tttcatgatg actgaaatag ctaaaataaa tatctcgttg gataattgct
     4621 gatactttaa gggatatcta aatttgctga ttgatttatt tatgttatta tgatattata
     4681 ataacataaa tgtgttacgc ccgaacctca ttttaaaaaa atgaaggggt gagacaatga
     4741 atgctattaa ttggcatgaa tttgttaact atatggagag cttactagat gatctaaaaa
     4801 gttttagtat tgctgatgca gtaaagcatg ggttaccagt tagtacaacg atgaaattac
     4861 gaggattgaa aattccagat gaaattatta agctgagaac taaaacaatt attgaaaccg
     4921 caaaagtcgt tgacaatatg aaccatgaac taaaaggcaa gaaaacaact tacgagaaag
     4981 attttgaacg ttttttaaaa tctgttattg gaaaatatct aggtaaaaaa tctttcagtg
     5041 aattgagaga tgaagagatt gtacctactc caatggttag caaaatccga aaaaacaaag
     5101 ccgaggggaa ttactatctt ggtacaatag caaaaatcat tgattatttg gaaaataaag
     5161 agcaaaagaa aggaacgaag aaaggaagat aatttttgtg ttaaatatta atggggaaga
     5221 atataccgaa atgattcaaa atcccgaacc tagaattacc gaaaaaacgt ctgattgttt
     5281 gctcttttat gaaagggggc agacaaacta atggaaaaaa acgtcaattc aacatctacc
     5341 aaagatcaat acagtattat tggagggttg cttggtgata aggataagag aacacgtcac
     5401 ggattacgca ttatgcttgc aacttttcta ggagtgcttt ttttattaac aggcttgggt
     5461 agctacattt atcaattagg aaactataat tattatctcg tgaacaatac aacacctata
     5521 ggctcttcat tggagtttgc tcgatcagga gcagagatca ctatttcaga tgtgtggaca
     5581 gatcaaaaca gagatgtaac agtagtaaaa ctcggttact cgcaaaacgc tagaaagttg
     5641 ttatctacgc aaggaaaaaa ctacaactta tatatggcaa cgaaagaaaa agatcgtcct
     5701 aaaaagcttg aaatgagcta tgggatgtta agtactgatg gggatgcttt cttattccta
     5761 aaaggtaaat tagatgaaag agcttatcaa gtttttattg caaaccaagt atcactaatt
     5821 tctggtgatg atacagctga cgatgatacc aaagtcaatg tgaatgaagc agattcagtt
     5881 acaaaagcac tttctcaata ttctatgaat gatgttaata atgaaggagt aatttctttt
     5941 gggaaaaaag caaaacctgt taatactcct gataatatca atttcagaat aaatccatat
     6001 tcagattcaa ctaatgtcta tcaaggaagc tttcttacca aagatggcga tattgattat
     6061 tcaaaagttg tagcagttac aagtaagaat aatgttgtaa gtaagttaga agatcagtta
     6121 aatgcttcaa atactaacat caagaaaatt gaagctacaa aagctgagta tctggaacgt
     6181 ttacaacaaa atcctgacga taaagattcg caagaaagta ttcgtcaact agacagtcga
     6241 atggagaccg aaattaaagc aatcaatgat acacaaaaat tgattgatca atacaaaaat
     6301 gctgacttta acaaagattc ttttggagac atgaagacga catttaagta cctaactctg
     6361 actgactaga agtaaattat tcttatttta gaggaagtga acacaaacag atggaaaata
     6421 gtaccgagtt ttttaagaat attcaaactg aatttaatca tgctctcaca ccaatgaaac
     6481 caccgaaaca taagttagtg ggacgtgatg acgaactcca tgagttgtca ctgatcttga
     6541 atagaccgga aacaccgcaa gctatcttgt taggtgaagc cggaacaggt aaaacagcac
     6601 ttgcaagtga atgggctgaa agacagcgac aagatggaaa gaaagtacaa gtatacaaat
     6661 tatcaatcgg acaattgcgt gttgggggag atgatgtcct ccaaacacgt ttagaaacat
     6721 taattccaag attgaaagaa tatgagcgaa ttttaaaatc tgaggacaaa gaaaatgaat
     6781 ttgtcctctt cattgacgag tttcataaag ttgttagctc ttttggtagt ggcaataaaa
     6841 ttgggggtga ttcgctaaaa gaaccgttag caaatgaatc acttcaattg attaccgcta
     6901 ctacaagaaa agaatatgaa aactatatca aaagagatga tgccttaggg agacggttac
     6961 aaccgttaga attgaatgaa ctaaaaagcg atgttgtcgt cgatattttg agacaatggc
     7021 taaatacatc ctctgttaaa gatgatcctc aggcttcttt taaattatcg caaaggattc
     7081 ctgatgaatt acttcaagaa atcgtacaag caaacaagct ttatcgtgag gatttgtccg
     7141 aaccggctaa atcaattgcc gtaatggagg atttgattgc aaaagatcga gtagacggaa
     7201 atgcttataa tcgagaaagt ctattgcaaa tatttggtaa gtataatatt cagctagatt
     7261 ctgattttga tatggaaaaa cttcaagagg aattaaagtc cataaaaggg caaccgttag
     7321 cagtttatac tataacaatg ttgtttaaac gtatgaagct tggatttgat cgttttaaag
     7381 gtcgaaagca accaaaagca actgctctgt ttacaggatc aacaggggtc ggtaaaactg
     7441 caatggctaa agccgtggca agaggacttt atggcgatga aaataaagtt ctcttagtta
     7501 atatgccgga ctacaaaaca aaagatgatg caatgcgttt tagacgactc ataggtactc
     7561 atgtaaggca tgatccttct gcgattattg tgtgtgatga atatgagaaa gctggtgtag
     7621 aaacactcga tgccttttta tcaattttag atgaaggtag gataagtttt actgaaagag
     7681 gatgggatga tcgagaagtt cacgacactg tttcattgtc aaatacaatt ttatttgcga
     7741 caacaaatgc aggtgcggag atgttcgatt cgttaaacag atatagctca ctatctcgtg
     7801 ttacaaacaa ggaaaattct aatgaatata gcactgaact aaaaagagaa taccgaaaag
     7861 tcgcatcagg gatcaaacaa gccttgattg gtgaaggtct aaagcctgaa gttattaacc
     7921 gatttggacg aatagttcca ttttgggctt tatccgaaca aactcttctg gaaattgctt
     7981 ataatgaaat gttgaagagt gctgaattag tacgaaaaca tttaaatata gatttacgtc
     8041 ttccacctag gaaagaaaga gtatatggtc aaaattttta tgcagacgat ctctccatgc
     8101 acttagtttt tgacatggct tcacaagagg attctaaatc agggggtgca aggaatatct
     8161 tgcatttagt tgaatctgag tttgaagaag cgattactga agcaataatt acttatccaa
     8221 acgcaaaaat attcgatgtt aagttgagtg aagccggagc gtttgaagga aaaggtgatt
     8281 ctaatgctga aattattgca aagccttccg ttgctgaaag taaagaaaac aagattgtat
     8341 tttcgattgg aggggcgtag ctatgaaatt caagaaatat ttactacaaa accttagaat
     8401 aaaacgatgg gtcgtaagag gtctagttat tggaggagta ttagcagtta ctttaggcgc
     8461 tccttttgga atgaatgatc caactcctga caatcaagat agcaaacaag aaacagttat
     8521 aaataagaac gttgcatatg cggaaagtaa accggcagaa caaggaatgt ggacactata
     8581 tgctcgtatc ctaatgggag ataaagaagt taatgatagt gccgctaaaa ctggacaaat
     8641 gaatttgtta ggacaaggtg gtatcagtgc cgatttccct tattctgaca tttctaaaca
     8701 aggtaattcg attgcagggg gtaatgcagg tactcaacta gcatctactt tagcaactta
     8761 tagttactac ggatatattg aaacagtctc aggaaatgct attgccagtc aagcatcatc
     8821 cattctagga agtattggta ggacgattgg tagttttatc ggatcaatta gtttaatcct
     8881 ttctaatatt gctgaatcca taaatgaact ccttggaaaa tttttcatga gtatgaatat
     8941 attttctctt atggggtggg gagaaggtgg atcggatgta tctaatccgg tatctaaagc
     9001 attaactagc ttgcttaaca atttgggatt aaataatgaa ttatttaaag cgatagcaag
     9061 cttatctatg ctctttttca cagcaatact aggttatcaa ggaatgaaat tattgtcgaa
     9121 agatggtgtt aactcgaagg gattatcacc agtacttgaa aaatggattg ttcgtatttt
     9181 tgtattgttt atattcttac cagtggcagg atcgctattg ggagtttata tcaacggcat
     9241 ccagtcagca aaaaaagatg gagatatacc aacaaatgtt gtttctgact acatcattaa
     9301 cacaagactt tgggcttcta ctcaaaatct tgctccatca tctgatactg gtggtgatgt
     9361 acctgacgga aagcctacat ccgaaatcaa gttcgttgat acagattatg aaccatctaa
     9421 aaaatctgca atgataagag cgatcaatac tagcagttat gccagacaaa ataatgatct
     9481 agattctaat tcagtcggta tggctctatt aaaagaatgg tcatcgaatg atactttcaa
     9541 tattaatact tatgccggtg atgttaatgt cgccgaaaat gatgcttata tgggaagagc
     9601 gcctgctgtt tggacaggct ataatttagg ggatggaaaa gaaggaagtg caattaagaa
     9661 accaaccaat ttaaatggat atatttggtc agcaaatcaa aatgtgacag acgacaatcg
     9721 caagcctgat ggaaaaggaa cagcgcataa ctttgatgct actcaacctt ggggcgttgc
     9781 taacaatgca acttttagca cacagtcggt agctttattg ctccaatcgg cacttgatac
     9841 atcgggagct aagttttatg cgtataatat cgctccttca ggaattcaag gcagtgctaa
     9901 gaatatgtca acagtaaaaa cagaatggcg tacagcatcc ttagttggtc atggagctat
     9961 gggtaagttt ggctcgtggc tagcgatgaa cgctgaatca atcgttttag cagttcttta
    10021 cttagcatgt gcttgggcga tgttcaatgt gaatatcgtg aaagcattta aacgtgaatt
    10081 tattcggatt tgggaagtag ttctaacagg atcacttgca tcaggagcag gtgtgatggc
    10141 aatttcatta ggtgttgtta caacatctct gatcggacta caattaccta tctttctgat
    10201 tacttttatt cgtgcatttg gtgatactgc tgtgggattc attcctaata cattagttgg
    10261 cgatggcgta gcaaatattg ttcgatctgc tatgatttta ggtgtaggat atcttttagt
    10321 ctttaaaaaa ggcgaaaaag taaataattt gattaatatg cttattggct taccacttga
    10381 agtagcgttt gggtttgatg aacgtgctaa gttattattt ggtaatagaa atgatgctgt
    10441 gcgagttgtg gacggcgttt ctaaagcaat caagaaaagc caaggagcta aaaatcccta
    10501 ttatgatggt ggcaacagct cagtaaaaga tatggttgca ggaggtcttg gagcagttgg
    10561 agcgcaaaaa atggctgaac gtttgaacaa tatgggagat agcaatgctg atgctaagtc
    10621 cattgctgat aaagtaattc ctgaaacacc agaaggaatg tcattcaacg gtggcggagt
    10681 tggttccaca atcaataata cctctttcaa agatttagtt cgtcaagatc aactacgtaa
    10741 taactcccaa caatatattg atcatttagg acgtatggtt gatgaaaacg gaaactttgt
    10801 cgatgaaaat ggtgaaatat taaaagatag taaatacggt aaagatgcta aaggcaactt
    10861 tattgacgaa aaagggcgtt tggtcgatga agatggaaac cttatagata ctaatggtaa
    10921 accattggaa gatagtgaag atggtaatat tggtatggat agcgaaggac gtttaattga
    10981 tgaaaaaggt cgcttagtcg atgaagatgg aaatcttgtt gacgaaaatg gacgcaggtt
    11041 gctaaatggt gattttggtc atgatagtaa cggacgttta gttgacgaaa aaggtcgttt
    11101 agttgatgaa gatggaaatc ttgttgatga gtttggtaat aaacttggta aacacagcga
    11161 tggatataat gtgaatggag atttagtcga tgaggatggg aatatcattg agacgagaga
    11221 tgcgcttgtg gcagatcgaa atagagaact cgaacaacaa cggcatacgc caattgctgt
    11281 tgcaattcct gagaatgaag aagacgagat tactttagat caaactcatc aagaactgag
    11341 gaatgaaatg agtggtgagg acgtgtcaga cagagtgcat cgggaattag gagatggagc
    11401 atctggtgaa agtcagtcaa gagagcttaa cagcaacttt gataatagtg ctattcggaa
    11461 tgacacagta cttaaagaca gagaatcgct aacaaaagaa acggataaag aacaatcaga
    11521 tgtgaagaaa aagttcattc ctactcctaa tgttaatcaa acagataaat atgaaacaaa
    11581 tattggatca ggaatgtcga aagacaatca atcaattaga gaacctgaac tgacaaataa
    11641 aaataataga acaaataatt ctaaatctac ttcagataat tcagggaaat ctaatcaatc
    11701 aaccaagaaa acatcaacag ctaaatcagc cgttgaatat ctatcaaaaa ctggtaaaaa
    11761 tgtttacacc tcagcagata aagctttagc aaaagctcca aaaggtagtc tagcagggat
    11821 aacctatgaa gctaaaaacg aagctattaa aataggtaaa ggaacggcta aggttgccaa
    11881 ggctactgct acaggagcat ccaaaactgg taaagtagtg aagaaatcta ccatgtcagt
    11941 caaggactat attgtaaaat acggtaatca aaaatcaact gaaaataagt ctgaagacga
    12001 gtaaaaagaa ggagcattga tttgctcctt ctttttactt tgatttctaa ctgaagatgt
    12061 caaggaaaat ttctatttat gcaaaaaaat tattttaaat tgtctataaa tgaattaaat
    12121 atttactgtt caaagttgta taattaaaaa gggcttatgc tggtaaagaa cagaattatt
    12181 atctaaacaa aaacttgata aaccagcatt tttagccaat aaaaaaagtg aaataattta
    12241 atggaaaatc tcttttaatc atcgaaaatt tatgttatta tgataacata aagatgtaaa
    12301 tatcgagacg tctttttctt agttagatca ttgacaagtg aacaaataag atttgtcgat
    12361 gatataatta acttattaaa aatattaaaa aggaggtttt tatatgttcg attctattaa
    12421 aaatttggtt agcggaatga cgcctgatat gaaaacttta gtacaagttg ttttgtattt
    12481 cttggcattc gttttttcgt tgaaaccagg attcactgca atgaaatttt ttggtgatgc
    12541 aaaatgggga gatggattaa aatatgttgg ggcgattatt gcaatcttta ttattcctgt
    12601 aattacaacc gttatgcttt acacaatggg tactaaaaca ggtaatgatt tgaaccaaaa
    12661 agctaatatc atatcagcac ttattccagt tgttggtatc tatctagttt caaaatttcc
    12721 aaaaaatgaa ctgttaggtt aaaagtgaat tgaacaaatt tggataatat gccgatttgt
    12781 tcaatttact ttttaccaaa aaactacgct ccaagcgtag tttagaagtg agggaagaaa
    12841 gtaaatgtaa gatggttttt gatagagaaa aaatgttagc tcacgccaac gaagtattaa
    12901 tgagcagtct gaaaggtacc gaactggcaa agataatgaa tatgaatgtt aaccagttct
    12961 atgattatcg aaatgggagt aaaaagattg aaaaagccag acttgaaacg cttattaaat
    13021 tcgaaaaagc atatgtatat atgctagata aacagaaaag aactattgac tgaaaaaagg
    13081 agttttacaa taatggcaaa attcaaagat attaccgaat ctattaatta tgtggcagaa
    13141 acagttcctg tagttgcact ctctttgaag ccacaattac tcgaagtgta caaaaaagaa
    13201 gaaaaagaag cgaaggcaaa aaaagctaaa gcaaaggtat ggaaagaatt cgatgcgttg
    13261 ctgaccaaag atttaccaaa gaaaaattat atggtaattg aagatgtaca aaagctcaac
    13321 agtgagggag ttatagatga ggatgatgct attctcgatt ctgagaagta tgtcgttcta
    13381 ttgaagtcaa gtttgaatta tgtagctgat ctaccagaaa aatttaaaga gaagctatta
    13441 gcaggtgtcc gttttatgga tagtagtggt caattattag ttattccgga tgatcctaat
    13501 gtgtttgaat cagaagttga agaagctaca gacttctata gcgcagtaga gcaattagaa
    13561 gtagctacta atggattaga acgaattgct gagaccttca actcttctga agaagttgaa
    13621 tttgaagccg tagcagaaaa tgtaccagat gaaaaagaag ctctttttgt ggatgataac
    13681 aaaccaaccg aagcagaaaa ccaagaagta agcatagaag aaacacaaat acaaacagaa
    13741 acctaccaac aaccaaaaat tgatgaaaaa cccgaatccc aaagtgacaa agaaaattat
    13801 gtttatgaag ctgaaaaaat cacatctgaa agtgatgtac cgaaaaactt agatgctgat
    13861 tttttagaaa aaaattttga acagatcatt gctgacaaat ttcaacctgt agttttagat
    13921 gagattaatg accctgtcga tttaaacgag gagatgctat ccatacctga gttgaaacca
    13981 ttgctgtttt tagctaaaga gagtatgaac gctcgcatct ctattgctaa taataaaatc
    14041 aacagtgaat taaataagac gaaggagcaa ttgatcctca aaggaaagca aatgttagct
    14101 gatgagcttt tgcggataga ggaagcaacc tcattaaatg gtcaaaatca gtttacaaat
    14161 acactatcgg ttgctaaaga gagctacgaa gcagaagcat ctaaaattga tgatttagtg
    14221 atggaaaaat ccaatatcat tcgtaaagat tatgatatgg cgaaacaggc ttatttggat
    14281 gctgaactaa agaaactaga gcaacggtat gatgctgagc atttaccaga agttaaacaa
    14341 caagaagatg aatatagaaa agcaactgaa gaaagtcttg atcaacagta ccaagaagca
    14401 attgaatcta ttaaaaacga tgcattgaaa caaagccaag aagcaaaaga agttgtggtg
    14461 gaacgaatta tcaattcatt gaaaccagaa attgaaaaat cattaacaga cgtgcaagaa
    14521 aaaactttag agattgtcga aaaaacaaag tcagaaaatg aagctgagtt ccatgataca
    14581 aaagagaaaa caacagatgt ggttgtcaaa tattttgcga acaaagaaga agttgaaaag
    14641 caggtagcta acaacacgaa agaattagaa acaaaacttg tagagcaaca aaaaactatc
    14701 gaagaatata aagatcgaat gaaagaggtc aaagatttag cagagggtta taaattaaat
    14761 agcgaaaagt tggcagaaca aagtgaagct ttaagagaag acttatctcg ttcccgaagt
    14821 gatctagctc aaatggcaga aaaaacgaaa tcattagcag ttgctacaga aaatcaatca
    14881 agttcaaaac gtgattttac aagtaaaaag atggctacta tcatttctgt cgctgcaatg
    14941 gcttgcggaa cgatcttggg atcgtcctac tttattagtc ataataatca acaagcacag
    15001 gctcaaactc aaacatctat ttcgagagtg gaaaattcga ttgggacaaa tacgaactct
    15061 gcttcaacaa atagtagcta tgaagcaaca ccaactacag agttcaaagt tgggaaagat
    15121 gttgtcatca gtattgatgg gaaaacaact aaggctgaag tgatttctat tgatggagaa
    15181 aaagcagttg tgaaaactcc agacggaaat cagtacaaaa ttcaagctca cttatctgat
    15241 aataactaag gagggctatt atgcgagaag aaaaaatagt ttatccagta attcaaaaca
    15301 atcaagaaaa catgacctct tctcagcact tacaggaagc acagctcgtg tatcaggtaa
    15361 atgaactacc acaaacgaac gagacaatga gcaagtcaat atctttaatt ggcttgctta
    15421 ttgttgcaat agtgataact gtaggtgttt accgtaagtt gaacgtgaaa ggaagtattt
    15481 ctgatggcag ataactcaaa ctatggattt aaacataact tacatcaaaa accttctgaa
    15541 acaattgtca gagttgaatg gcttgaaaag catgtcaata gcacctattc tgttacagca
    15601 aaaagattaa ttgtatcagc tatagttgtt gcagtcgttg ttcttggatc aattgtttac
    15661 ttagtttctg atacgattaa actgaaattt atttttgttc caacgttatt aatgataatt
    15721 ggatttctta ttctaattag ccagtatatg tttattgatc catctagtgg ttcattgatt
    15781 tctggttcta ttagactgtt caaatcagta gttaattcag caagagtatc tatggggaaa
    15841 aagaaaccat ttcgaggcac agagatttta aaagtgaaac aagatggaac aatcgtatat
    15901 cgagatggga gtgtagcatt aatgttcaat ctagatgggg ctacatcact aaccgctttt
    15961 ccagaagaag ttatggctca ggaatcgaaa tcaatacaat atcaaaatac cagagatcgt
    16021 tccttagctg aaatcaaaat cacttcatct caacctcaga atacggaaag acaaactcga
    16081 aatatgaaaa tacaaaaacg aaatgcaagg agtaacaaag cagtctttga cataatagat
    16141 cagcaagaac gatttgtatc agaatatatt gatggtgtga aaacaacagt aatccaatat
    16201 cttatcttga aatctcaatc taagatggta ttggatgaag gtgtggaacg tttagaaaac
    16261 tatacgagta gaggacttta ttatagtgtc actcagttgt cagctaaaca aacaaaagaa
    16321 atattatctg atatttatgc actgaaatag aggtgagaga ttaaatggaa attatgggta
    16381 gatcacaact attaaatttc aaaaaaaaga gaaaaactaa aaaagaacaa aaaactagaa
    16441 attcaaaggt agtgaataat acctttgtta gtgatttaac atctttagtt ggttatcaat
    16501 ttacaccatc ttacattagg acaggtaagc ggtggggaac aattgtgaaa ttagtgaata
    16561 cttatggaat gaatagaaat tatggatttg gttggatggt taaagctatt cctgaagtga
    16621 gagtagaagg ggtaaaagcc tatctaattg aagcagataa gccagtgtca caaaaagatc
    16681 aacaagaaat aatgcacgag aatgttgggg gatcaacgaa tagttatgaa aataatttag
    16741 aaggaaaaaa ttcagatgct gaaattagca aaactgttat gcggattatg gatttgaata
    16801 cagcatcttt actagacagc atggcaaata aaattattga ttggaagcta gaaatcctat
    16861 tagtatccga ttctcctaat aaaatcagcg aacagctaat taaactccaa cggttgtatg
    16921 atgaaaaaat gtatggatta aagcttatgt catatggagg agatcaacgt gatctgtttg
    16981 atagactttt aagtgaacca atcgggaatg tctatgagta tacgtctatg agttcggatt
    17041 ttgcaggaaa tgatcatgca attcgcaaag gtttagatga tgcgaatgga tgggctatcg
    17101 gcactatgtc tactagctac gcaagtggtg tagcttttat ggcactggat gattctttta
    17161 caagtaaagt tttagtggca tcacacaaaa acgctacaat taaaggctat gatgaaaaaa
    17221 tctcagcatc atcactttgg gggcaattga ttgctaataa tgcaatggca aatcataaac
    17281 gaacttttca tattgtctta aataaatttg attactttgg tgatccaact ggtagaacat
    17341 ttgttgccaa accttcgtta aacagtgtaa tagaaaaggt taatttggct gaagaaggtc
    17401 ttaactcttt ggagcttttt ggtaatcaaa aggacattgg tgatttatat agacggaatc
    17461 tcgataaact aactcagatt ttttatttgt tagcaggcag aacattagat tctgcaacta
    17521 gagttttatt gaaaaaagaa ttaggtaact tttatataag tcagaaactt tgggatatta
    17581 attatgaaga acgttcaaaa cttttggtga acagtagtga tacaatgcca gttcttgggg
    17641 attttattag ctatttgact aatgctgaaa caaaagccaa aaaatctggt actgaaaaag
    17701 aagtcgatcg tattattgta ttaaaagaca cattgagaac agcattagac agcttctcta
    17761 ggatgtttaa tcaaagaacg acactgaaag aaatcggtaa aagtgacaaa ttacagtttt
    17821 actataactt aggtgatcta agagcagaaa ccaatgttat ggaagctcaa tttttaaata
    17881 cctttgattt catcacatca caagcaaaaa aagatgatat tgttatgatt cacggcttcg
    17941 ataagttatc tattgaaacc gttgggctaa ttaaaaaaag attaacaatg cttgaagaag
    18001 atggaattcg tcttgccttt ctatttgata caatcggttc aggagaagac aatgaataca
    18061 aagaagatat tgaaacagct gatatattca atacagatgg aaaaatttac caaagttttg
    18121 aaagtgattt taattacact atcattggaa agatgaccag tggagagtta atgaagtatg
    18181 aaaaaaaagt tgctcctgct agaaaaggag aagatgatca aaaactttct gttgatttga
    18241 gacagtattt gacaacagga agaagtaata aatttcagat caggagacca gtggatctga
    18301 ctaataattt tgttattgga gagtttattc tttaagtttt atctttgatt ttgagtgtat
    18361 aaggaaagga taagaaaaaa catgtatatt tgagactaaa aacagtctta gagaacatgg
    18421 tacgttttgt gaaaagagaa ctatagaaat ttcataaagt gaaaccttac tgagtgaaat
    18481 aattatagtg gaggttaaat aatgaaacca aacataaaaa ttttagatcg aatatttcta
    18541 ggtagagaca cagaagtaat cctaattcaa catgaagaag gatttgaagt tagtataggt
    18601 attcagaaac ttcaaaaacc tcattattgt aatcagttgt ataaaaactt tactgatgaa
    18661 gaaaaagcga gagttt
//
LOCUS       DASJST010000049        18627 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJST010000049 DASJST010000000
VERSION     DASJST010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.049"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..923)
                     /locus_tag="U4B05_001358"
     CDS             complement(12..923)
                     /locus_tag="U4B05_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2050509.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1300..1938)
                     /locus_tag="U4B05_001359"
     CDS             complement(1300..1938)
                     /locus_tag="U4B05_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050510.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1942..2712)
                     /locus_tag="U4B05_001360"
     CDS             complement(1942..2712)
                     /locus_tag="U4B05_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN2050511.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3130..3546)
                     /locus_tag="U4B05_001361"
     CDS             complement(3130..3546)
                     /locus_tag="U4B05_001361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050512.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3597..3978)
                     /locus_tag="U4B05_001362"
                     /pseudo
     CDS             complement(3597..3978)
                     /locus_tag="U4B05_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4104..4769
                     /locus_tag="U4B05_001363"
     CDS             4104..4769
                     /locus_tag="U4B05_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050513.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5166..7199)
                     /gene="brxL"
                     /locus_tag="U4B05_001364"
     CDS             complement(5166..7199)
                     /gene="brxL"
                     /locus_tag="U4B05_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HEN2050514.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7317..9839)
                     /gene="pglZ"
                     /locus_tag="U4B05_001365"
     CDS             complement(7317..9839)
                     /gene="pglZ"
                     /locus_tag="U4B05_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HEN2050515.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9955..13587)
                     /gene="pglX"
                     /locus_tag="U4B05_001366"
     CDS             complement(9955..13587)
                     /gene="pglX"
                     /locus_tag="U4B05_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HEN2050516.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13651..17322)
                     /gene="brxC"
                     /locus_tag="U4B05_001367"
     CDS             complement(13651..17322)
                     /gene="brxC"
                     /locus_tag="U4B05_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN2050517.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17347..17922)
                     /locus_tag="U4B05_001368"
     CDS             complement(17347..17922)
                     /locus_tag="U4B05_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HEN2050518.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17922..18521)
                     /locus_tag="U4B05_001369"
     CDS             complement(17922..18521)
                     /locus_tag="U4B05_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HEN2050519.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5739 a         3561 c         2610 g         6717 t
ORIGIN      
        1 atttataccg cttatttact acttgctgta ctagaatctg aagtgaatgt actcaaaaca
       61 ttttcaaagt ctttgtcttt gatttttaca ttagcgtctt tcaatacttc gccaattact
      121 ttattctgga aggtactgtc acttaatttt gtgtctgttg caatttcttt caattctttt
      181 ttgtatttat ccatgtcgtt gcctttgttt tggtttttca ccattttaac cacataatat
      241 tccgtagtat aagttgaagc attggtagaa gtgatcacat cagaaacttg accatctttc
      301 aacttaaagg ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga atcaaatttc
      361 acttttccgc catcttcttt tgtcgtagta tcagttgact tgtctttggc taatttagag
      421 aaatcgtctc ccttatcagc agctttcttc acatcttttg catcatcttc acttgctact
      481 tggataattt gagcttccac ttctggatgg aaagattccc aagccgtttt taaatcttca
      541 tcggtcaatt tgatatgttt ctttagacct tcttgaaagg ctagactttg tttgatactg
      601 tctttgaatg tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa tgaatcaccc
      661 aattgtttct ttgtttgatc atattgggaa tcgatttctt tggttgatac gtcatctcca
      721 tatttatctt caaatacttg ataaacgatc aactgttgca cagcggaatc tgctccttgc
      781 atttttttca agctatccat tacttcttgt tcgctgattg taccgccttt ataactaacg
      841 gctgtttttt caccggcatt cgtacagcct gacaataagc cagtgcctag tacgatggca
      901 agtgctaaaa atcttttttt catttatcaa ttctcctact tttcgttttt tgcgaattgc
      961 taaaaacaaa ggtgttcaag atgaaaatca tcaagaacac ctttactaat ctgctcgata
     1021 cgtcctgaat agatataagg ctacacaatc cagaaagttc gctcagcaga ataaatgtca
     1081 agtagcacat cacaccttaa tatactactt cttttgttac cgtttttcaa gtctatcttg
     1141 cgttccgtat ttaacgtaac taattcttag aacaaatgtt atcgattttg tttttcttct
     1201 tccgctgttg ggatattcaa atcagacact tttatgttcg attttccaga catttttttg
     1261 ttgtttttat tagaaaaagt ttgttttttc atttgttttc taaagcatga tcttcttgtt
     1321 tcctattttg gtcagatatg gatcatgcgt gaccacgata actaacgtac cttgggaagc
     1381 ctgttgcgat aagatttcaa cgacttgctc cttatttcgt ttatctaatg atccagttgg
     1441 ttcatcggct aaaatgattt tgctattctt caataagact ctcgcacagg cgattctttg
     1501 cttctcacca ccagataatg tgtagatagg cgtacttagc ttcttgttta tatttacttt
     1561 tttcaatgcc tcatactttg ctgttcgctt atctgaaaca tttaaattcg aaaacttcaa
     1621 tccaatatct aaattaaaat cgattgtttc atattcaatc aacccaaaat cttgaaaaag
     1681 aaaagagagg gcatttcttt ttatcaagcg cttctctctt ccagttaaat gttttgtttc
     1741 ttgattaaat aaataaaatt gccctgaact gaattgatca ttcaatccta atatattcaa
     1801 taaagtactt ttccctgctc cgctctctcc aactacagtc acaaaatccc cagcatccgc
     1861 agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg atttttttcg caccttttat
     1921 cactatcaac ggctccaatt gttaacctcc attcatcagc aataactgac ttttcttttc
     1981 tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg aagctaaaga aaaaaatatt
     2041 aaaaagatat ttaggctctc tttctataag aaaaaggaag attacacttg tcgttcccca
     2101 tttgatgagg ttcatcatga agaaatcttt atacgtttca aagagcgaat aaccacttga
     2161 acgaaagata gcgattttcc gacaattgta gacaaaatat atgcataaca tgacatagct
     2221 caacaagaag aaagaaaaga aagtgagtaa aaatatctgt atcaagtaat gaaatgatct
     2281 gcttaacact ttgacagcta tctcttcagt ctgagtatac ggaaatgata actgcaagtt
     2341 atcacgtaac tgactttctt caatcagctc aaataacctt tctttcgaca aagagcctat
     2401 cttgatcttt aacggtggat agatttctcc gctgagaata tttctttccc aatacgtact
     2461 gtttttcaat gtcagtattt caacaaccgg ataatgttcg atccagggga tagaaggaat
     2521 aaatgtataa atgggttgat tgttttttat aaatagaaat ttcggttctt tttcactgat
     2581 ttcttgataa tattctgtta tttgcgccag gtcatttttt aaatctgtat gtctatatcg
     2641 ttcagggatc aatataacag ggtgcgtttc tttttcggtt atttggatca accttccaga
     2701 gacatcaatc aaaggaaatt tcttaagata attcagattt atttttatcc ctctgccaaa
     2761 ctgttcattt tcttcattca agtaatctga tacattgact aatatacttc cgtttttatt
     2821 caaagtttca tataattcct gatcggctgc tgatgcaaaa cgattggaat aaataaaatc
     2881 cacgtggttt ttcccgatgg ttactggata aaatacatga tagtcctcac cagcgatttc
     2941 ttttggtgtt tcatgggaaa cagctaactg tgaagaagaa aaaacgatag ttgctaattc
     3001 gacattggca acggtcaaaa gaagagaaaa caacaatcca aatgtaaaaa gaatgtattg
     3061 gatatagttg aacgcttctc ttaacgaagc gattttttca actaataaaa gagaaatcaa
     3121 agagataccc tatattccgc tgaacaagaa aacgtacatc tggaacaaat aaagaaattg
     3181 ctccttataa tacataaaaa taaaaccacc aatgattcct gcaattatga atgtaataat
     3241 gattttcttt ttccccaata aacaccacaa tacttgatgt aatgaaaagc cattattcaa
     3301 caataaactg atttctttat tcatcaataa gaaaaaggat gataacaaaa ggaataaaag
     3361 aatcaatcca atagttaaat acactcgata attatatacg tctgtacttg aaataaccga
     3421 atacgaatag ctattttcaa agtcttcaaa aatattagtg gcataatcag tgtgaaatgc
     3481 ttgattgaat cgttccacaa atagtaaaat gaaccgttca taggcttctt tttctctcgt
     3541 catcaagaaa aattcacctt caaaatcttt tattttgatc gattcttcaa tggacgttac
     3601 gcgaaaggag gtattagaat agggtacctt ctcgtgagaa gatttgacat ccccttgata
     3661 atacaatgtc atttcaagct ttggctgtat cttgaagttc caagatgtat ctacttcatt
     3721 ggattcattt actgtttttt tcatataaga aagctcagtt tctgatgaga cattttcaaa
     3781 taccatgatt agctgctcat attcttcttg cccaataaaa tgttgtggca atcgaatcgg
     3841 agtataagta tctgtttgat ggaaaaattc atttgtccga tagagtacct gtttatttat
     3901 aataaccaag gcaaaaccac tcatgatacc ggtcactatg attgtgagta aaactgtgaa
     3961 cttttttttg attatcacca ctctccctta cttaaatgat tctttgataa aatcataccg
     4021 tttttcatca aaaaaacgta tctatcaaat gtataggtca tgtagaataa atctatatac
     4081 tgtagaatcg aaaggagaag aagatgaaca tcggagaaaa aatacgaact taccgcagaa
     4141 attgtaagct tacgcaaaaa gaattaggcg aaaaaatcgg tgtttccgat aaaacaatct
     4201 ctagttggga aaattctcgt acaatgccag accttgaaat gctgtcactt ttacatcaga
     4261 ctttaggtct gccgattgat tttccttcta ttgaatcagc taccgaatca atagaaccaa
     4321 cattgccgga tgaacctctt gttatcaaga aatcttattc atcaaaacaa cccttctcac
     4381 tcaaaaagac actatctttc tttctgctcg tcttttgttt ggtctatctt tttcttatca
     4441 attatcaata taaaaatgaa acggttgatc gattcaatcc ttttttgaaa ccacaaatcg
     4501 gctataccac actccctgta ggatacgtgg aaaattccat cgaacattgg gtatgtaatg
     4561 atatctttgt tcatggtaca tggctgtctt ttcacgctgg agaggtagaa aaaggtaaga
     4621 atcatgctgt aatcaaacac aaaggaaaac acgtcatgaa cgttcgcctg ctcacttggg
     4681 aagccattcc ctcagattac caagaaatcc tcatccaaga ctatgacgat gccttaaaaa
     4741 gaagccctca ggagccagat aggcattaag taggagatga acgtagagat agaaaaaatc
     4801 aatgcacatg atcttatcaa aaaaaaacag gggtacaaat taattgaaaa agctttgaga
     4861 tgaaagttcc taaataaaaa agttcagaat gataggtaaa aaaatgacta actattctag
     4921 tattaggcta tttgtattat tcttctgtta tttttatatc aaaaaatcca gaaataacta
     4981 aatataggta tttctggctt atagtataat atttacgcca cagcccccaa tgattctttt
     5041 tggaatcatg atcaaggacg gacatacaca aaaatatttt gcccattcga tccgaataga
     5101 ttaacgacta atctagtaag taatatagat tggtacgtag tcaaaccagc aaatggaaaa
     5161 gttgtctatt ccaccccaag ggctttaaag acagcatctt ccggcgtttg ataaaaaatc
     5221 aaattaaagg tacccattaa ttctgggggt acagttccaa tatcagcagc gcttatcata
     5281 ggtacaagta cttttttcgc tccggcatct tttgctactt gtaaacagtt agctaaatta
     5341 tctaccttaa tcatagaacc accaattgta atatccccta aaataactaa acttggtaat
     5401 acaggtttat ttaaggaaac tgagcataaa gctattatag ttgataacga taatgttttt
     5461 gtcataccaa tgccattcat atcttgaaca tgaacgacaa aatcttttgt cgtcaaacta
     5521 atagactgac taacacttcc ataattagct cttaaatatc ggtatgccga atccatcgct
     5581 tctcttgctc cagcatctga attgatccca gtacgttcaa actttccatt tcccgaagtc
     5641 atttgcgttt ccaatttata ggtacctaat tgcccagttt tgccataaga taccgtgtac
     5701 aaatgccctg gcttccctaa cccttctgga atcaaagttc ctccaccttg ttcaggaaca
     5761 gaaacatact cttctcgctc ttcttctaaa tcaatgtatg aaaaattgac atcataaaat
     5821 tccattccac caattttctt taattgttct tttacacgac gacgcatttc aagagcaaat
     5881 cttagtattt cttcgacatc acttttttca aatctaccat caggatacaa cagtttcatc
     5941 atcccagata caatccgacg aactgcaatc gtatcccgct gatttaattg agaaccaagt
     6001 ttgaaatatt tatcaaaact atcgccaaaa ctaacttttc gaagctcacg catgacttca
     6061 gaaaaataat ctgtaatgaa gccatagtca tttgtaaaaa aatctggtcg atatttagga
     6121 atttcccaac ctggtaaata acaatgcatc cgatccaaaa aggctgaatc cgtccccata
     6181 acctccggaa atggctcaaa taaattcgaa gttttcaata ttacatcaac gctttgatta
     6241 atatttccta caaaagccat tgatgctgaa gcattctttt cttctttgcc tctagcaaaa
     6301 gatcctgaag ccatgtaatc tttcatgatt tgaacgccat cattatcctt aaatttaatc
     6361 ccagcaactt cgtcaaaagc aacacagtcc cacataccta caagtcccat tgtacgacta
     6421 gccatattat agaaaaggtt tgctactgtt gtttgtccac ctgaaacaag gattgagttt
     6481 ggtgaaattt ctttatatac atgcgattta cctgtaccac gtggtcctag ttcacagaga
     6541 ttaaaattat tttcaattaa tggaataagt cttgctaaat gaagccattt taccctttta
     6601 ttaaattgtg taggttccat accaatggac cgtagaagta agtcaatcca ttcatttgta
     6661 gtaaactcct tgcgagccat cttgtaagcc tcaatgtcta aaccaggcat ttggattgga
     6721 tcaacctttt ttgcaataaa agggctattc caacgatcat tctcatcata ccaatattcc
     6781 atcgaaacaa tacaccagat ccccccacct aataatcgat caaatttttt cggatactct
     6841 tcactaattt gaacattttt taatcccagg ttagagaatt ctgcttcata acaatctttc
     6901 ttttcattta aatgtacagt tactttatca ataactgtat aaccacccaa ttcacgaatt
     6961 tttgctttta ctttttctgc ttcatcagga cgcacatagt ttcgagcaag aatattttta
     7021 acattttcca cacctttttc aaccaatgtt tcatccaatg acgaagcata cataccaagt
     7081 aaatattcca atacataaac aggtacattc gcaccttctt taattttctt tgttaaatct
     7141 tttctgacaa tctttccggg aaagaattca gataacttta aaatttcctg ttgttccact
     7201 agaattcctc catgtgttca gtcattataa tagcaattaa agtatatcat acttaaccag
     7261 taggcttgat aatacaaaag aaaaagtatc cacaaaaaat tgtggatacc tcgtttttat
     7321 atatcaaaat caaatgattc tgcaaaagga ttatctataa tgaattttat tcgctcggtc
     7381 aaatcatttt tttgattttt tatcactaaa taataagtat cttcaactgt atatcgacga
     7441 tttacaaatt caaaaacaaa tttagtaaac cgctgatttg catcttctgt ttttgaatcc
     7501 gcaacataga tttcttcatt tgagatacgt tcaccatatt catcttcaaa ataaagccag
     7561 aaagatgcgg ggtcataaac atcactaatt gcattttttt gatagaattc taacgtggtt
     7621 gataatcctg taattctgcg gttgctagtc attaattcaa ttgctactgg ttcctttgca
     7681 ttgctacctt tggctaccgt aatttctaga acagggacta gcatttcttg aatagatgac
     7741 cctccatgaa cgtagctttg tcccccgcca gctttcttaa aaatacttga agtaatcggg
     7801 acacaaatgt ttctagtgtc atcatttcct aaagaatctc ccaaagagta atttttcgta
     7861 ccaattattt cgtattcttc ttcactaaga ataaaacgac gttctacacg atctttttca
     7921 tttctagatg gattttcaat cttatctact tcttcaacgt ggcttctttt ataaataaat
     7981 ccatgatcag ccgtaactat aaaacgatag atactgccat cattatgcaa gcgattaata
     8041 agtcgggaaa tttcgcttat cgctaaatca caggcctcaa aaactcgatt ttcattcgag
     8101 tgatcacctg ttctatccac ttgatcgtga taaatataaa tgactgattt gccattaaat
     8161 acttctcgta cctcagcctt attcatctta accacatcat cataatttaa agctacagca
     8221 tttgcatctc gttgctggag aatcgcattt cgattggata aaccattcgt ttttttccca
     8281 tcaatcagta cttctgttcc ttcattgtaa gttagttgat tgtttggtaa taaggcggct
     8341 ttaccgaatt cagtaactga aggtaataca gaaagaaagc tagccatgtt tgttgaatac
     8401 ttcttttctc taactaactt acgttctaaa ttcatcccta actcatatcg aaaagcatct
     8461 gaaataataa ctacagtttt aacagagcgt ttcattacag cttgattata aaattttcga
     8521 atggatggac gattcttaaa ttcaaaagtt tgattccaaa tttgtcctga ttgatcaaga
     8581 aacttcgaat aatttgcttc aactaatcga tacaattggc catatttttg cttttcttct
     8641 atatgatcac tattccaaat aaatttccga taatgcgtat caacccaata catttccttc
     8701 tcatattgag ttagcaaatt atccatgttc atcggcaagc acaatctgtt tttatccaca
     8761 agataataag catgtttcaa taattgatac tcataaatat aatccgatgc gaaatgcatt
     8821 ttttcgcgct gctttaaact atcaaaaatt gtttgtcctg caattttcgt tgtagtgtca
     8881 gaagccttta atcgttctgc ataaaacatt aatatttttg catgaatctc agaaaatacg
     8941 tctgcttcaa ttagatcatc aatttctata ctgtctaata atccatcacc atcaatagcc
     9001 tgatatactt ttttagatag ctgttcaaag ctttccatgt aacgtgtatc attcataaac
     9061 tgatccatga aagtaataat gtttgctttc aaatgtagta tttcaaatcc tttgaacttc
     9121 ttcggcatct cttgatcaat ttgataataa aaatagttca aataaagaca catcgtcagc
     9181 ttttccaatg ttggctggct atccgtaaag ccaaatttca tttgaataaa ttcccaaaat
     9241 ttagctgtta actgatactt atcaagttcc cttaaaggat tctccctact ttctgtaaag
     9301 ctttttagaa ctacacgcag taaatcattt aatcgaatac ttttcgattt caacaaaaca
     9361 gccattatca ttaattcaat cgtttctgat ttattaattg tcatatgcgt actaaattca
     9421 ccaaaacttc tcatgcgatc ctttgcatta aagaaattac gatactgttt aactgtctga
     9481 tgaaatctaa gtggaatagc caattcattc atgataatcg ctaagcggtc tgcactaaat
     9541 tcctctgaat aattcaatac actcaacaaa tgattatctt catctttatc atcccaacga
     9601 ggaaatggcg catagattaa atagttagtt tcgggatctt catattccaa taaacgctta
     9661 gttttaaatt gtgcgtcctt ttctaatcga tagatttttg cattttgaat gaccaatgag
     9721 tcaatttctt caagaaaatc ctgatttgaa tcaaaccaaa aaatgatttt cctacatcca
     9781 taattaggaa aacgttgatt taatttttct tcaatttgtt gcgtatttat atctgtcatc
     9841 aatctcacct aatcgttctc tttctatacc ccttatattg acataatttg gataatttcg
     9901 cacgcaattt aagaataaaa aagaaagttg ttagaaaaca actttcttag ataactattt
     9961 ttttggttct ttgacccctt ttgcaaaaat ttggaatttc tcgccggttt taggatcacg
    10021 ctgcaactta tcataattga caattacacc atcatctaag tctaattcaa ttcgttttaa
    10081 tgctaagtga cccaacttct cgtcatactc acgaacttct tttaattgct tctgaatctt
    10141 tacaatttct tttgtcattg catttctttc ttttgggtta tcacttgttg aaatttgttg
    10201 ctgacgcaat tcgattcgat tgtcatacgc tttttgtagt tcgtgtaggt attcagtacg
    10261 tacttttcct aattgatctg gtgtatagcg atgcaagtac attaacgctt taaagccatt
    10321 ttgttttcct gagtccaatt gccaataaat tggtcgattg gagtatgttt ttaaatggtc
    10381 tttgtaaaaa tcttgtacga agtaacgacg aataatttct cgacttgtcc ctttgccacc
    10441 taaagtgtca gcgataaact ggagattttc ttcaagggtt tctttaccaa aagtaatctc
    10501 aacgaaatca atgaaacgat tgacaatgtc aatttggcta tccttgaaat actcatcatc
    10561 ggtgatgagt aaaacattat cagtatcagg tttaaatttt tgatagcggg aaggatccca
    10621 ttcgccgcca gcgaagatga gaccatcttc gtctagggag taacgaccga agatacaacc
    10681 tattgaataa gaaataaaat ccttaatttc cctcgcctta tcaggtaacc ttgcagagat
    10741 atattcatct tgaacctctt ttgttaattc tttttcaaga gaataatgat taatgaatat
    10801 gtcgttcagt tcctcttcat tcgattttag agtattaaat tgattaacca actgcctact
    10861 tagacagtta aatgcctctg agagatcttt tttaaacata taacttaaca aaatgtttct
    10921 tctaaactcg tatgacgttt caagagcatt atagtctttt ctagctatat cgatattttc
    10981 atccactttt tctcttattt tttttaattt tctctcagat gtaactttaa gaaaaggtag
    11041 ttttcccatt ggtccttcat ggtaatccaa ggtgggagaa ataaaagtta aaaagtgatt
    11101 aactaattta gagtttacat agccaaatat ttcttcaaag tcagttatat cctttgagaa
    11161 cagaacagat cctttagttt caaaaattgc ctgattctgc aatctaaaag atacttgtcc
    11221 agatgaaagt gtcgaccaag taattccctt tttaaaatag taattaagat tttgtggtct
    11281 agattttaac ttcccatttt cattaacata attttttatt tcatctccat cattctccca
    11341 gttaaccacg tattcattgt ttccatacca ttttctaaaa cgaccacctt tagttgtagg
    11401 aaaccatttt tttctagact ttttagcgtc ctctaatgtt ttagcatcaa actttatttt
    11461 gttaaattct acttcaaacc aataccgcaa aaatttattg ttatttccgg ttgccaaccc
    11521 ttgttttgca tctgcaacat cgttagttaa ttttccttga gcaaaatcag tgattacgtt
    11581 atcgcttgcc cagtaagcaa tcggcattcc tgggattttt ttgaagttgt cttgattagt
    11641 actataaaaa taatcgcatt caccatgctg atgaattgct tctaacgttt taattttttg
    11701 aacttccatt ccacctttaa aatctgataa ttttatatag cttccatctt gttcatttga
    11761 attttttatc gtaaacgtac aaattggaac ggttgcttct tcaaaagctg aatactccat
    11821 ttgaatcaaa ctagaaatgt ttctttcatt aattaaaaat gagcgtaact tttcatagct
    11881 agaaatgaac atccaaacaa atggcgtcat aaaggaagca tatccattat caatcagcat
    11941 ttggctattc aatttaataa acacagaaaa tagatctttt ttgacatcag aatagttatc
    12001 atttacatat ttttttaatt tcttatccat tttattaaga taaggcggat tagtgactac
    12061 cgctacatat ttatcaacta gcatttcagc cacgtcaaga atgtgcaagt atttttcttg
    12121 tatttccttc atcccataaa gaatatccat attcgtaaat tcgttgaatt ttgatatata
    12181 ctctcttact tttcctaagt attctttagt tattgcctga ttatttttat cgactaactt
    12241 aatgattgat ccaagttctt tcccattttc aaattgatca aaaattaact ctttgtattc
    12301 attccagcgt tcactatact tatcattgtt caattcacta atacgctctt gatactcgtc
    12361 tgtcacttta ggagaatcaa taaactctct taaattcggc gatagacttt ttttcaaaaa
    12421 aagacgatcg tattgacgcc ctttcatcat aattgcaaaa taagacaatt gatacgctct
    12481 tttatcgatt tcaagaccat ataaattgta ttcaacgatt gcacgtgcag catcacgctc
    12541 agaatatcct aacgtaagat aaatttccat caatacatcg aaggcgtaaa ctaaaatatg
    12601 cccagacccc attgcgttat cgataatttt tagttcttct ggttttaaat tacttacaga
    12661 tgttgcttgt tcaaaagcat atttatctgg aattaagtat ttcaacttat ttcttaattc
    12721 actattggga taatgatcta accaatactt tcctaaacta ttgtcaacca tataacgaac
    12781 gacccaatct gtggtaaata gttgagttgc tgccggaata tcttgttttt tgacaatccc
    12841 tttataaata ttcaccactt gattatgtgg ctcactatta tagtactgat acaaccaccc
    12901 aataatctct acctgtccac cagcttgaac atagaaatca tcctctggaa tacttaaaag
    12961 atcagcgatt acaccatctt ttttaatata agaagctgta aacaaaagtt ctgtataatc
    13021 ttctacttct tcaaatagct caggtaagta atcattcaat tgattacaga tttttataaa
    13081 aatcaattga tacagcttgt ccattgcttc tgctgaatta tctgttttcc aagttctgac
    13141 ttgttgctgt tcattactag ataattcttc aaagatatcg gcatcataaa tatctgtaat
    13201 aatatctggc tcaattttcc cacttatttc actagataat acacgatacg tgtcaggtaa
    13261 ataatcattt acctccataa accgaatggc aatcaatcgg ttaaaccacg tataagcaac
    13321 ctcttcgatt aactcttgaa aagcttctga ataagaagtg acagaagcac gttgttttag
    13381 ttctgcaatc aatttgtttc tttgcgctat ttcttttcct tgaattctta ttcctgattt
    13441 ttcgatttct tgtaaattgc tatcaatttt cttcacatct tcaatttttg attcatcaat
    13501 tcctaaacgt tttgctttag tcattacttc atcaattaat tttctacggg cattgaccgc
    13561 gaaattcttt atcgctttct tatccatttt tctttccctt ctatatgtaa cctttgaaaa
    13621 gacaggcacc tttttatgcc tgtcttttca ctagaattga atcgtgattt ctgtattttc
    13681 ttgaagttgt tccttcagtt tttgattaat ttcagctgtt aactcatcaa tatctgctgc
    13741 tgacttaata gttttaacat gaacgttaag tgttctcatt ggtactgttt tctgttgaat
    13801 agtaggtttt actatcgcta tttcttcagc ttttgctgca gagtctattt catctttttc
    13861 agtttcacga atcaagtttg ctgcttcaat ttgtttacgt gcttgttctt cttgacgtct
    13921 gcggttttct tcattatcga aacgttgatt aaagatcaaa tactgttctg ctacgtttaa
    13981 tgataagaaa acaagatcat ctatagtatt cgcttgttca atttttgctt tcagttcttg
    14041 aaattcttgg tcaactattg gtacaaattg atccttaaaa tcttttcctt ctaataattt
    14101 aaatgttgct tgtttttctt ccgttaattt atttaatatt tcctgacttt ccaactctaa
    14161 cagttgatta tattgttcaa taaattcttg aatcaactca tttagtttca tcatatctga
    14221 ataaggatca gcctttttca aaatggtttt catctcatga atcaattgat caatttcttg
    14281 gctagaagtt tgacgccgac ttttttcgat taatctaatt ttatcttgtg cattctccca
    14341 gatttttttc tgagtactat tttgcttgtg gaaattttta attttatcat atgtttcagc
    14401 ccagtcgaat aattcatcgc gatatttttt gattgtataa aaaaagtctt caacatctga
    14461 cgtttgaaca attacttcaa gatatttttt tatttgctca tattcataag cactagggta
    14521 ggtataagtg ttttgtaaat caaaataaga gcgtaaattg tctaattgac cttttaaata
    14581 tttcttgaaa ttttccattg tatgttcatt gttattttca tcaacaatat tacgactttg
    14641 aaacagttct ctaccgattt cgtcaaccaa tttttttgct tttggcgaaa tcaccgcgct
    14701 tggttcaatt tttatctttt cactttctcg tcgtttttga ataatcatta caatactatt
    14761 tggatcatcc atccgtgaaa tttgtacatt gttataaagt aaatcaattt taccagacat
    14821 gaaaagttta gcgatgcacc attccacatc aagttctgtg aatccatacg gttgttttgt
    14881 ttcaaaacga tcacgaattg ttttcatcgt aatcgttagg tgatttctct tttgatcatt
    14941 gataaaattg aaaatttcct gaactgcttg ctcatttgat ggatcttcaa taatttcatt
    15001 tttgctttct ttcactaaat ataagatatc ttctttttct ttaggcgcat caatttggtt
    15061 taatttatgg tatacttctt cagccatttt ctgaagtgct gattggaatt ttgctttaaa
    15121 gtcggagcct gtcagttgaa tttctgcacc gttatagaaa aattttgcag atttcaacag
    15181 ttcatttaat tgatcgttga tacttgcact tagttcctgt aattcaccag atttttccga
    15241 acgaatcgcg tcaaagttct ttactacact cgaataagtc gttgttaaat aaagattcat
    15301 tctagcatac attgctatat catctttaaa ttctttatct gatggcaatt ctacgaataa
    15361 taagttacgg gaactcttaa gcgcaagttc tgcctcttga cctgctaaat cagagtatgg
    15421 tgaaataaaa ttaactgtca tttcagcatt tccagcttgc ttgtatggac gttgatctaa
    15481 cacttgtgca taagtaaacg tataccggtt tttaaattta tttccacctg acatgcctaa
    15541 tttttcatag gcaaaactat ttcggtctag aacgccgtca aaaattcgtt tagatagttc
    15601 tacgatcaaa tcactatctg taatattttg atttttaatc tcacgagtaa tttcctgttc
    15661 ttcatcagtt tggaaaatat aactatctaa gttcttccgc acaaagtttt ccttaattaa
    15721 aaggtccaat gcttcttcta ctcgtttttt agtttccagg cggtcttcgt tgatatttct
    15781 tatcatcaag ctcgtaatat tttcaacatt tggtttgatg gcttgatcaa catatttaat
    15841 catgaataaa attcgtaaga catttacgtt aaagttatct atatcagttg gtctttgaat
    15901 atgagaatta tttgtagctt gtgtaatcac tcggctaatt gctgtatcaa tccaacgttc
    15961 taacgagtaa taaaactgat ctaaagaaac caaactggtt gtatcatttt ccttatttcg
    16021 ttcagcggct tctttaaaca ttgctagtaa tgaacgttca ccagacgata agtttgatcc
    16081 ttgatagctg tgattacgaa tctcggttag aatttgcccc aataaattga attgataggg
    16141 aataaatgga taaacttctg cgtaattttc agcatcttgg aagagcttta attcaggact
    16201 atcaacaaag agtaatttat ttttcaattc tgtttgaaca gtttcaaatt gtaaggctaa
    16261 agaatcattg gcaaactctt ttttcttcaa aatacgttct ttaattactt catcggcatt
    16321 tgctgaagat aaactcacag ttgtggcaaa acgatccttg atcttagaaa aatcattatc
    16381 agaattgtta tcaaagtttg ttacgtgatc aatcgcttcc tgacttgtca ctaataccca
    16441 agccttacct tcacaaattc gacccaaatc ttccacaacg gtttgtaact gcaaagtcaa
    16501 ctcacgatta ccgccgatat attgtccgac ctcatcgact agaaaggcaa tatgatggtc
    16561 tgcttctttg gaatcaagat agcttttaac tagatttgca aagtcatcaa tgcttattaa
    16621 ataatttcct gatgcttttt ctaaaaaatt atcaatagcg gcttcactca taaaatcgat
    16681 tttaagtgcc gcttctttga tatcatcttg gatccaatca aactcatggc gctcttcttg
    16741 ccattttgcc ccattaatct cttcaaaagc agctttgaat gcatcaaact tttcttcttt
    16801 aattaaccga cgttcaaaat ctgctagatg tggaatcgtg cttaatcctt gcatttcgtt
    16861 aaataccttt aaaaaaacat tcacgattcc gtttttgtct tttttaccac ctgcatcact
    16921 ttttgaatca atattaaaca gaataacatc tgtatttatt tgtgagcacc gttccatcgt
    16981 tgccaaagtc agtggatctt tgattttccc atcttctttg aagtaatcaa tggctttctt
    17041 gtcatttatt tctcgatttg cgagcaagta agaaataatt tttaagaaat gagatttacc
    17101 tgaaccaaag aacccctgaa tccaaacacc cattttatct gttggtgtat tgatactccg
    17161 ttggtaggca tcaaaaattg ttttaaagtg tttttgtagt tctttcgtaa ccacgtattc
    17221 ttctaattct tgttgaacat tttcatcctt tgcttgaccg accttaataa ctccttgaat
    17281 atttcggtca attggttttg caaaaatatc tttaattagc atttaatctc ctaccttcca
    17341 tcccaactaa attctaaatg ctcgataata attatcatct ttattggtat taagaattgt
    17401 caaacttaat tcatcatatt cccctggata catcatcaca actggtacgc gatcaaatac
    17461 ctgatgcatt ttatttaaaa tagagtgcgc tctaattaat ggatataatt cgccaattcc
    17521 tgtcaggaac acaactgatt tttctggtgt atgggcatcc acatattcaa aaaatacatt
    17581 ttcttcctca ggtaaatcca ttttcaataa acgaccaatg ctttccacta atttttgtaa
    17641 accacgtcgt tcttccatct taatcgcttt ttcagtgaaa ccgcgttttt ctagatactc
    17701 taacataatc acatagagat tataaacttt cacttcataa ttatcaccaa cagatgttcc
    17761 agatatatca ttaatataat ctcttacaat taactcgtct ttcggatcgt agtcaaagat
    17821 ataatagcct acttcattac ctaaaccacg attcaataaa aaatcttctt tttgaattac
    17881 ttcattcaac ttttccaaat gctcttgaat cgataatgac attataaaat tcctcccagt
    17941 gctagtacaa tatctttacg attcttgtca actaaccatt gaatcaatcg tttatctaat
    18001 aatatcggag tcattcgtcc atattctttt tcccctctga ctaatcctgt ttcaattaga
    18061 ttcgtttgat aagcggcaat aagccgttta atcgtataat ctttccattt tgaaatatct
    18121 tctgaacttc tccgcttctc tgtaaaaaaa gaacggtaat caactggtgt taattgcaaa
    18181 tttcctttac gtagattttc agcatatacc tcagtcataa attcattaaa aagatcatca
    18241 atcataagga tggaaatcaa attcactaac ttttgattat ctatatttaa ctgattgaaa
    18301 agttgcaatc cagcttcatc cagttgacta actctccttt tcacgttctg atagatttct
    18361 ttttgacggt tactgctcac tgcaaaaaaa atattttcat gttcattttt ctctttaatt
    18421 tctatatctg tatatccttc tagtcgtaat tgaatatata acttgaactc tgaaaaccaa
    18481 aagcgtttag aaactagacc agcagaatat tttttattca atagattgcc tcctaatcat
    18541 ctataattca ataagtaaac aagtgttttt tgctcatcgt tagcattcat tttttagtat
    18601 ctactataca atacatattt attatag
//
LOCUS       DASJST010000050        18594 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJST010000050 DASJST010000000
VERSION     DASJST010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.050"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..334)
                     /locus_tag="U4B05_001370"
     CDS             complement(<1..334)
                     /locus_tag="U4B05_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050520.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFA"
     gene            complement(331..1005)
                     /locus_tag="U4B05_001371"
     CDS             complement(331..1005)
                     /locus_tag="U4B05_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2050521.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2181..3479)
                     /gene="asnS"
                     /locus_tag="U4B05_001372"
     CDS             complement(2181..3479)
                     /gene="asnS"
                     /locus_tag="U4B05_001372"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN2050522.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(3519..4709)
                     /locus_tag="U4B05_001373"
     CDS             complement(3519..4709)
                     /locus_tag="U4B05_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2050523.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(4730..5257)
                     /locus_tag="U4B05_001374"
     CDS             complement(4730..5257)
                     /locus_tag="U4B05_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN2050524.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(5304..8093)
                     /locus_tag="U4B05_001375"
     CDS             complement(5304..8093)
                     /locus_tag="U4B05_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2050525.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(8240..8440)
                     /locus_tag="U4B05_001376"
     CDS             complement(8240..8440)
                     /locus_tag="U4B05_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2050526.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(8893..9213)
                     /locus_tag="U4B05_001377"
     CDS             complement(8893..9213)
                     /locus_tag="U4B05_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050527.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(9716..10834)
                     /locus_tag="U4B05_001378"
     CDS             complement(9716..10834)
                     /locus_tag="U4B05_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050528.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11305..12612
                     /locus_tag="U4B05_001379"
     CDS             11305..12612
                     /locus_tag="U4B05_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN2050529.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            12733..13932
                     /locus_tag="U4B05_001380"
     CDS             12733..13932
                     /locus_tag="U4B05_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2050530.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(13959..15227)
                     /locus_tag="U4B05_001381"
     CDS             complement(13959..15227)
                     /locus_tag="U4B05_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN2050531.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(15397..16038)
                     /locus_tag="U4B05_001382"
     CDS             complement(15397..16038)
                     /locus_tag="U4B05_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEN2050532.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(16447..16950)
                     /locus_tag="U4B05_001383"
     CDS             complement(16447..16950)
                     /locus_tag="U4B05_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2050533.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17073..17837)
                     /locus_tag="U4B05_001384"
     CDS             complement(17073..17837)
                     /locus_tag="U4B05_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN2050534.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            17945..18220
                     /locus_tag="U4B05_001385"
     CDS             17945..18220
                     /locus_tag="U4B05_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN2050535.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5314 a         3953 c         3316 g         6011 t
ORIGIN      
        1 gggcaaaaat tgattttatt cttctttttt tagcttccag catcaagtat tctgcaaatt
       61 ttttttggat ttcttccgta ttttcttttg ttactaatgt gtgttggatt agttttcctg
      121 atggtcgctg atccacccgt tcttctgact gttgcacgtc cgcttgcgca aatccgataa
      181 tccgtttttc ttttgttaca gcaacgagca atagtttatt cttatcattt aggtcagctg
      241 ccgtttttgc ttttacgaat gattctggat gatcagtaat cagctgattg atttgaggta
      301 aatcttcttt tactgcccac cgtatttcca tcatttctca cttttctttc gctcattttt
      361 atctccacta aaaaaatcga ctgcggcaat tgcatctatc cagctttgtg caatgatctc
      421 atgtccgcca atcgttgggt ggaccccatc ttctcttgta tatttcgaaa aaccataagc
      481 aatccctaaa gcattgaata tgccatctaa aggaaccagc aatgcgccat attccttagc
      541 aagtttacga acaatttgga ttttagggtc taagtcttta cgccagccat gacggtcgtc
      601 ggttatagga agtacaaacg gctccatcaa gatgatgttg cagatttccg ctgtttgaag
      661 ttcatttagt aattttcgat aaacggattc aaattgctgc gcagatttct cagttccgaa
      721 tatagcctga tcgctagtat tatgccaggt atcattgatt ccgatcaaaa tcgatacata
      781 gtcaggttgt aaagctaaac aatcttcttg ccagcgtact tgcagatctg caattttgtc
      841 tcctcctatc ccacggttca tgcattcaaa tttcatcgtt ggatattggg agattagttt
      901 tccggcaatc aaaagtggat aacctttacc tagatcagtt gcttctgctc ggtctcttcc
      961 tgcgtctgta atgctgtcac cgataaataa tatacgtttt ttcatttgtc aaaaactcct
     1021 ttttgaataa aacgatgatt ttcttcctta tattatccca taagccctat caagacgaaa
     1081 gagaaaatat atgtataaaa aatgacacca gcctagtccc ttcaatcaat tgaatgaacg
     1141 ctcatcaagt gtctagtttt ttatactttt tttcttaacg tgccggattc cccttgattc
     1201 cttcgttgtt tggtccagag aaatcgatca cacctaagct tccgtcaatc agaccactga
     1261 tgacgccatc agaacatata tttataggct catctaagac ttccagtaaa tgttctttta
     1321 ctaatatctc ttctagattg cttgggatag attggtcata actttatctg gtccgatgat
     1381 ttcaaggatg aattaaactt acatatggat cattgcatgc tgaaaatacg gacctactcc
     1441 atcttcgaat gttactatta gtttgttttc aggtactaga tgctgcttta tttcaacagc
     1501 agaagcatca tcaaatcaga tttccatttg aataaccttc tttgtatatg tttgattgat
     1561 tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt tcttttgctt tcttctcttg
     1621 tctgctggaa acttccttta tcccgtattt taaaaaaaga actgcgagag ttcgcagttc
     1681 ttttttttaa ataaacgttg ctctccggcc aactcctcgg tcacaagtca caatagtcat
     1741 caaacatgaa aaactgtttt ttgaaattac tccttgtgac tcggagctga acggcaggcg
     1801 cacaacctct tgatataaac ggtgttcaat cagctgaccc tcgatattag ctaaaaaaac
     1861 ggtaaaatat gagaagctat ttttccgttt tgtttctcta atactcaggt caaggcactg
     1921 atttcacaac ctcttcctta aacggtgttc aaaagccagt ttctcggtat taagccaaat
     1981 ttatgcaaaa atgtggaaag ccatttccac aaaatttttc ttaatactcg aaactaaaca
     2041 gctttttcac aacctctcca ctaaacggta ttcaaaagct tacatctgcg gtaataagtc
     2101 ccgccaattc aaaaacatga acagctgttt ttgcttggct gttctaatta cttgatgcag
     2161 aacagctttt tcatcacctc ttatggataa attcggttca acaaacgtgg gaatggaata
     2221 gcttcacgta catggtcgat accagcaatc caagtgactg ttcgttcaag gcctagacca
     2281 aaaccagagt gcggaacaga accgtattta cgtagatcaa gataccatga atattctttt
     2341 tcatccaaac cgtgattacg gatttgttct agcaagaaat catagtctgt tgcccgttcg
     2401 cttccgccaa taatttcacc gtaaccttct ggtgcgatca tatctgcaca aattaccacg
     2461 tcttctcttg ttggatgtgg tttcatgtag aatggcttga tggcttttgg atagtttagg
     2521 ataaagacag gctgttcgaa tgaattcgca atgaacgttt catgaggtga accaaagtct
     2581 tcgccccatt cgatgtcatc aaatccattt ttcttcagta attcgattgc atcatcgtaa
     2641 gtaatacgag ggaatggcaa ttttgtataa gtttttaaga cttctttgtc tcgtcctagt
     2701 acatctaatg catattcgca atgatctaaa acattttgta ctaagaatgc tacatattgt
     2761 tcctgtactt ctaaactttc ttcttgatga gtgaaagcca tttctggttc gatcatccag
     2821 aattcgatca aatgacgtcg tgtttttgat ttttcagcac ggaaagtcgg accgaatgaa
     2881 aagacttttc cgaaagccat agcagcagct tccatgtata attggccact ttgagataaa
     2941 taagcttttt gatcaaagta atttgtttcg aatagatccg tagtgccttc tggtgcagaa
     3001 ccagtcaaga tcggcggatc gattttgaca aaatgatgtt gattaaagaa ttcatacgtt
     3061 gcacgaatga tttcatttct gatctgcata atcgcatgtt gtcttgaaga acgcagccat
     3121 aaatgacgat gatccatcaa gaaatctgtt ccgtgttctt ttggtgtgat cggataatcg
     3181 tggctctcgc ttaccacttc cacatttgta attccgattt cataaccaaa ttttgaacga
     3241 ctgtcttcac ggatttctcc agttactaac acggatgttt cttggtgtag atttttagct
     3301 gtttggaaaa cttcttccga cacttcgctt tttacaacta caccttggaa aaaggctgtt
     3361 ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc gcttatttgc gacccaagcg
     3421 ccgattgtta ctgtttctcc cacatggttt ttcgaatcga tgatttgaat ttgttccacg
     3481 tatgactctc cttttatatt ccttgaattt cttcaggatt aattttgact tttactctca
     3541 ataaattgct tgattctgcg taccgcttct tctaacgtag cgcgatcagt ggcataactc
     3601 atgcggacat tttctggtgc cccaaagcct tcgccagtca caagcgctac ccctgtttct
     3661 tctaaaagat cttctaccca ttgtgtgaca ttttcatact tacacatgcg tagcgtttct
     3721 tttacattag gaaaaagata gaaagctcct tgcggctttt caagagaaac acccggcagt
     3781 tctgccacaa gcggatataa cgtattcagg cgttcttcaa aggcgttccg catttcttct
     3841 actgtatctt gctcaccttt caatgcttca acagctgcat attgactgac tgctgtcgga
     3901 ttgctagttg attgggaagc aacagcgatc attccattga tgatcacagg atttcccacc
     3961 gcataaccaa tgcgccagcc tgtcatcgca tatgttttag acacgccgtt gatgatgatg
     4021 gtctgatttt tgattgcttc tgaaatcgtt gcaattggag taaactcatt gccattataa
     4081 actaaacggc cataaatatc atcagcaact atcaaaatat cattttttac tgcccattca
     4141 ccgattgcct gcagttcatt cttgctataa atcattccag tcggattcga tggagaattg
     4201 atgattacgg cttttgtttt ttctgaacgt gcttgttcta gctgttcaac agtaactttc
     4261 cagctattgg attcttcccc tttcacaaag acaggaagtc cttctgccag ttttacttgc
     4321 tcaccgtaac tgacccagta aggcacagga atgatgactt catcttgcgg gtcaaggatc
     4381 gtttggaaaa gggtatacaa agcaaatttc gctccgtctg ttacgactgt ttgtgaaggt
     4441 tcatagacaa gtccatagtt tttcttcaga taatcaacga ttgcctgtcg aagctctggg
     4501 attcctgctg ttggtgtata atagctagct ttcccattgc ggattgcttc gattgctgct
     4561 tcttggatgt tttctggtgt cgcaaaatcc ggttctccca cagtcaaact cagcacatct
     4621 ctgccttttg cttttaatgc cttggccttt gccgcagctg ccaaagtgac agacggttct
     4681 aatcgttttg ttctttttga taactccatc ccattcattc ctttaccttt taaattccct
     4741 tgattgtctt gacttcatct ccagattcaa agccaatcag gtaataattc agtttcccat
     4801 ttgtttcttt tcctactact tcccacacag gctgatcatc atacatccct aaagcagctt
     4861 tttcgatttg gatttcagga tgggccgcag caattgtctg ttttgcttcg ttttcagtca
     4921 gtccctttgt ttgatccaat acttttactt tttcaccaga tttaggaatg ataacggcga
     4981 tttcttttcc tttatcattt gtacctgtca gtgaaaagta tgtcttcttt cgagtaaacc
     5041 aataaaaatg atcggtttcc tcaagatttg catatttctt tgcgatttct actgcttctt
     5101 tcttcgcctg caccgccgga cgactggaac gcaagtaaaa aatcgagctg atcacgatga
     5161 ccgctaataa aaatacgatc aagccaagta acacgcgatt aatcgttttt tcctttgtct
     5221 gttttttctt caagggtcgt cgctccttta tcttcattcc ttttcattat agcagatttt
     5281 aattatgaaa aaagacagct cctctaactt ccttcttcat ttttctttaa gaattcatgg
     5341 atatcctgat agatttcctc catagctagc tctttgatcg gtactttttt cggtaatgca
     5401 tggatgatcc gtgcgccata ttttgcagtg actagtcttc gatccaaaat aagcaatacg
     5461 ccacgatctg tctccgtcct tatcaagcga cctaatgctt ggcgtaactt caaagctgct
     5521 ttaggtacgg attcctggta aaaaggattg atcccctctg attctaaata agcgttccta
     5581 gctttgacca tcgggcgaag cggattatcg aagggtaaac gtgtgactac aacgatctgc
     5641 aaagcatctc caggcaaatc tacaccttcc caaaagctgt cagcgccaaa caatacgctt
     5701 ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc ttccaccaaa cccttgagct
     5761 agtatttctc gtcctttttc tagcatagac agatgcattt tctgatagac cttctgcaaa
     5821 acatcatgag aagtgaataa cacaagtatt ggccgatctt cttgttcgat cagacctgta
     5881 agtgctgtct ctaaataatc gatatatgct tgattgctga cttgatggac ggcaggtgcc
     5941 tcttttggca catagatcct tgcctgtttc tcataatcat aaggagaggc aatcacttta
     6001 agaggtgctg cttcaatgcc aagcctctta gagagatagt gtcgattagg gccgatcttc
     6061 agcgttcctc caatataaag aatacgatca tatcgaggat accaaacagt cgacggcaac
     6121 atagccgctt gaaaatcatg gagatgaaaa atcatctgct gcgctgtgtt cccaggaacg
     6181 aaccaatgga tcaaatgatc tcgccagtct tccagccatt gatccatcag caagtattgc
     6241 ttgtctaatt gatcaaatag gctatataaa cttgctaaat cgatccgcat ctggtttgtc
     6301 cagctttctt ggataatctc aaaagtcaga cgcatttgtg actgtagctg ctggatctcc
     6361 tgataaaaaa gctgcaggtg atcaatggct gtcccagatt ccagtggcag ctgatccaac
     6421 atctcttttg tcatcatgac ttcttctgga tactgtccgt taggttgctt gccgctcact
     6481 aaactcatta atccttctgc catacaaatc tgtgcttcgc atagcgcaga aagttcttct
     6541 cgataaatcg ataaccagcg taaggcttca tgttctttcg gtaaatagct ttgtattcgg
     6601 tcaaacagct gatttggttc ttctaattgc tggatttgtt tatggaaata gttaatactt
     6661 aatcgtttgt catttacttt ttcagcgatt tccggcaaat gatgtacttc atcgatcaac
     6721 aaaaatggac tagctggaag caatggttct tttcgctgtg tttcttgtac taaaaacgca
     6781 tgattgacaa tcaaaaaatt actttgcctg attttctttt gcaagaaacg aacaaagtct
     6841 tgttcgtaaa atgactgatt tcttgctaaa aatccaatac ctctgtgcgc gatatctgca
     6901 aagaatgaat ggttcaaatt tgttaaatgt aattcatcca aatcaccagt cttcgtttgc
     6961 gtcagccaaa ctaagaccgc catttgatac aaagcatatt gtttttgttc tggcggttcc
     7021 acaagtgtgc ctttaaaacg ctgcaaatca atatagtggc gatagctttt gataattaca
     7081 ggttgtatgg gctgatctag caattgattc agcaatggaa tgtctttttc tatgatctgc
     7141 tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt tttcaggtgt agccaaataa
     7201 ctcaatggca gcagataacc gattgtttta cccattcctg tagctgcttc gatcatcaaa
     7261 tcttttgatt catcttttgt gaaatggtcg tagaccgcat tcatcagtct gttctgttct
     7321 tttcgataca tcagtgtttt accaaacatt ttttcttttg cttgtttttt tcttggatat
     7381 gtccgctcgc cataatggac ggatgtgaat agttccactt cttttttctg caaagctagc
     7441 ccatcgacga tttctaatga gggatcaagt ggctctggtt tttctttcat ttcttcaatc
     7501 acatgataaa tgaaacgact cgtatccata cctgtgtgtc tacttaatcg cgcaatctgc
     7561 tctaacgtga tgattggcaa ttttctcatc cgttcttcga tcagcaaaag aagttgacct
     7621 gtcacttcgg catcactatc tgcctgatga ggattctcat gaataaatcc aaggctttct
     7681 gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga aaatttgtgc aagttctact
     7741 gtatcgatgc ctggtatctt caaactagga agtccgcatc gcatgagttc atggttcaaa
     7801 aaattataat caaaataaat attatgcgcg acaaatatgg tgtctgccaa aagattgtaa
     7861 atcgtttggg cgacatcttc gaaataaggc gctttttgga cgcgttgatt cgtgatccct
     7921 gttaaatgct ggatttgttt tgaaatcttc cgccctggat gaatatcgat cgaaaaacgt
     7981 gaagtgatcc gacctgattc aatcatgacg cagccaaatt ggatgatccg atcttctttt
     8041 ggattcgtcc cagtcgtttc gatatcaacg actgcataag ttcttggttt caaaatatct
     8101 tcctcttctc ctgcctctaa tgttcttttg aaattatact acaaagtgca gaaaaatact
     8161 ctcatcgctt tttaaagaaa atagacaaaa acgcccagac acgcaaatgt ctgggcgttt
     8221 attttttagt ataggttaat taagctttag taacgtttgt tgcttgaggt ccgcgttggc
     8281 cttcttcaac atcgaaagtt actgcttggc cttcttctaa agttttgaat ccgtcgcctt
     8341 ggatagctga gaaatgaacg aatacatcgt ttccgttttc tgcagtgatg aatccaaatc
     8401 ctttttctga gttaaaccat tttactgtac ctgtttccat aataaaaatc ctccttgtgt
     8461 atcaacacat attttgcaat tacttgaatg gtgtatatct ggaagatgtt tatttgaaac
     8521 aagctaccac agattaccga acaaaaacta ctcctatagt atatcatacc gtttacaaaa
     8581 aagctagagt ttttcgactt tttttattaa aaaaagtttt ctcagcgatt acataaagtg
     8641 aaaaaccgaa ctttttttta aaataaacga catattgttc agttggtgtt tttttgctct
     8701 gatgttcttt tttaaactaa aaaaattatt ctttctgact acttatcggt tctgtttcaa
     8761 cggtcgaggc tggggaagat tctttagtag gtctgatgtt ctcatggtat ttctgatagc
     8821 ctatgacagt taccagaaga acaaggcaaa ccaatacgac caacgagatt agtttttctt
     8881 ttttgctcat atctatacct cataaataac aggatgatgt ccatcatatt tctgagtatg
     8941 agtaaaagca aaagctgcag ctgtagaatg taagaaatgc cagcaacttt cataatcaca
     9001 ggtcggacag taccaaaagt aaccaaatct tggctgcaaa gaccgttctt ctgtttcagg
     9061 aatcgcagaa tcaagatctg gtgtctgtac ataaacattt ttgtacagac gtgtgcctgt
     9121 aataggaaac cgctgtgttt cttcagcaaa aactggctga cttgttaaac ttagggatag
     9181 agacagcaaa caccataaca gtcttttttt cataaagacc tccttatcga taagtttttc
     9241 ccaattaaaa tcatatctca agaacgcccg ttattcacat tttgttcata aatgatgttt
     9301 tttgtatcat aatcgtcaaa aagaggtcgt gaccacgtca caacctcttt tttaaacggt
     9361 gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaaataatt
     9421 tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct cttccttaaa
     9481 cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat atgaagagct
     9541 atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac ctctcataca
     9601 cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgtgaaacta
     9661 tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac ctcttttaat
     9721 aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc aaaataaaca
     9781 cttttccgcc attaatgatc acatcatctg ctaatgcatt caacacttga cgccgatcat
     9841 attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg ataaataaat
     9901 gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg atgaatttct
     9961 tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt gacagcttct
    10021 tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa atcgcaacgt
    10081 ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt tctggtagag
    10141 caaataaata aatcggccat tgttctggat tttcgaaaga atccttcaag tacgtatcaa
    10201 ctgcttggta atagttgacc caatcgatct ctacttcttc atcttttgga tttacgccat
    10261 ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc aaaccgccac
    10321 ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct ggtagagata
    10381 cttcttttac ttgtttgttg ctgacttcat acaatttcat ggagtcgcgg ttcaatccca
    10441 ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa taaggaagat
    10501 cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag gtttggtcag
    10561 cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga tctcctaaca
    10621 aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt ttctcaaaac
    10681 gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc acatcttgat
    10741 gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct tctgaagtca
    10801 attgtgacaa taattcttta tccttttttg ccataaacgt acccctccta aaaaaatcat
    10861 tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata aaacgtatct
    10921 aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt tatcttcata
    10981 atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct gataaactaa
    11041 gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt ttattaagta
    11101 ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca cagcctattc
    11161 ctgtaaaagt acgtaaaaac gaccgctaac cttccataac gtgcgttctt atcctaaagt
    11221 tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt gtagcgaaac
    11281 aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt cctattattg
    11341 gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca agctgaagat
    11401 actttcaaag taaatgcaaa agcagccttt gctgtagatg cgcaaagcgg taagattctt
    11461 tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat catcggtctt
    11521 tacatcgtcc ttgatcaagt aaaagaaggg aaactatcat gggatgataa agtttcaatt
    11581 tcagattatg cagaaaattt gagtgtcacc ccagatttat caaatgttcc tttgcataaa
    11641 gaaaacacct acaccgttaa agaattattc gactctgcaa tcatccagtc agccaacgct
    11701 tcaatggttg ccttggcaga aaaaatctct ggcagcgaag caaaattcac tgaacgaatg
    11761 aaagaacaat tgaaagattg gggtatcaaa gatgcaacga tcgttaatgc atcaggactg
    11821 aataactctt atcttggcga aaaccgacca gaaggtactg gagaaaacga cgaaaatcaa
    11881 atgtctgctc aagatgtggc tatcgttgcc cgtcatttga tcttggattt tccagaaata
    11941 ctggatgtat ctagcactac gacacaaatg tttggcgaaa acacgcaatc accagtagaa
    12001 atggtcaact ggaactggat gctcccagga ttcgtgaatt ataaagaagg cgtggacgga
    12061 ttaaaaactg gtacgactga acttgcagga gcctgtttcg tcggtacgat cactaaaaac
    12121 gatcaacgaa tcatcactgt cgtattgaat gcagacggcc atgcggaaaa cccaagtgcc
    12181 cgtttcaatg aaacagctcg cttaatggat tattgctacg acaattggtc agaaaaagag
    12241 cttggaaaag ccaatgcaag tatccctagt ctaaaaacca ttgatgtaaa agacggaaaa
    12301 gaatcaacag tgcccgttgt gcttaaaagt cctgtaaaag tatgggtacg gaacgatatg
    12361 gatactggaa aattaacaat cacaccaact cttgacaaaa aacaagtcac tgacaatgag
    12421 atccaagcac ctatggataa aggaacaaaa atcggtaccg caacgatcac cttggcagaa
    12481 gataaattag gttatcttga agacaacaac gcgccatcca cagaaatcat cacggataaa
    12541 accgtagaaa aagcaaatat tttcgttatt ggctggcgac gcgtcgcaga atttttcggt
    12601 aacttgtttt aattgaacaa aaagaaaaaa gcatctttcc atgtggaaga tgtttttttg
    12661 ctttttctag tttaaataac agcttttttc atctattttc gttatactgt ataagaaaaa
    12721 ggagttgaat ttatgaatct aaccagagat ctgatttttt tggttctcta tcttattggg
    12781 gtattctcca tcgtttattg gcgaaaacag aaaaaggatc cttccttgtt cttttacagc
    12841 agtacatgga cgtttgcggt aatcttgact tattttgtcg tgttggaaat tttttattcg
    12901 accttttatt ttttgattcc cttagtgttt ttcagtatct ttgccttttc ctatttttca
    12961 gaaaagcgaa ggctgttgaa tggtctttta ttcaatattt tcctcattag ttttggtatc
    13021 tacttgtttg tcttgctata cgaaacacaa aatatttttc ttggcggact gattgccttg
    13081 atcacgatcc cgttactgct tgtctttcta ttcggcattt atggattgat cgtttttctt
    13141 ttttggaacg gtgtcacagt tttacgtaga gagtcgcatt cattagctaa tcttttaacg
    13201 ttgatactag ccatttttct tacattgttt cttgtttttg actttttctt gttaaaatat
    13261 ttaccacaat ggataaatgc tttgtttttc tgcatcccat tgatcttgat ctatctattt
    13321 atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg ccctcgatac
    13381 aaccaagact tcatcgttgt gttaggcgcc gggttgatca atggagaaac agtttctcct
    13441 cttcttgcca agcgaatcaa taaggcgatt gctttttatt gggcacaaag ccgtgcgaca
    13501 ttaaatccgc ctatcctatt aatgtcgggt ggacagggag cagacgaaaa agtaccagaa
    13561 gcaatggcga tgaaacaata tgccatggaa caaggaattc ctgaaagaga tattcttgtc
    13621 gaaaccaatt cgacaaccac tttggagaat atgcgctatt caaaggaaat catggatcag
    13681 caaatgaaag gtccctatcg cgcgatcttc agttccaaca attaccacat tttcagagct
    13741 ggtctctatg cacgacaagc gaaactgaag gcaaatggca ttggggcaaa aacagctttc
    13801 tattacttgc ctaacgcgtt tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa
    13861 agacatatga tcatttgcgg attgcttttc gcaggttccg cattcttatc actgatcact
    13921 ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt tttttctttt
    13981 attctcttga cgattggcaa gaatagtatt ccttttttga cggctacttg ctgtgcagaa
    14041 taaataccgc tgtttcctga acacataaag ctgatcaagc agatcataaa gaagtaagca
    14101 gcagcttgtg aaccaaataa ttcgattccc atcacaaaac aggcaatcgg tgtatttgtc
    14161 gcaccagaaa ataccccgat aaaacctaat ccagcaagaa aaggaacaga aacatggagc
    14221 aaaggcgcta gtactgcgcc aagcgttgca ccaatctcaa atagtggtgt cacttctccc
    14281 ccttggtacc ccgcacctaa agacaagacc gtgaagaata atttcccaat aaaatcataa
    14341 gcatgtgcct gtccattaaa tgattcctgc agtaacggaa gactcaaccc aaggtaacgt
    14401 tgtgtttgca aaatcaacgc tgcggctaca actactgctc cacctatcgt atttcgccaa
    14461 acaggatgag gaatccattg cgcataccgc gcttttaaaa agacgatcga acggctgaat
    14521 acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa cagcaacata
    14581 ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata gctttctgtc
    14641 atgaaatttg caaaaagcgc agcaaagaag ctaggaaata ttgcttctgt tcggacttta
    14701 ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc aaatacagaa
    14761 ctaaaacccg cactgattcc actgataacc aaaatctccc gttctgcttt atccaatcgg
    14821 aataaatgcg ctatattatc agctaatgct ccccccattt gaactgctgt tccttcacga
    14881 ccaacagatc ccccaaataa atgggtagtg atggtcccaa acaaggtcaa tggaatgagc
    14941 cgcaaaggga tcttctcttc tcctccattc ccttgatcga tcactaagtt attcccacga
    15001 ctagaaagac cgccatgata cgcataaaga taagcaaaaa gcgcgccgct gactggcaat
    15061 agataaagta gccaaggatg tgctagtcgg atatctgtca cgaacgtcag gctatttaag
    15121 aaaaaagcag acaagctccc catgaatact ccgagaatcg ctgtgaccac cagccacttc
    15181 aacatgtaca tcaaaatacg cactggactc ggtaattgtt tttccataaa aaattcctcc
    15241 tcaaataaaa aaacgcctac taaagaaagg ggctggcccc agactttagt aggcgtcatt
    15301 agctatctta cgttagcatt taaaggcgaa caccatcacc tattcacttc cattaaaaga
    15361 tagcatattt ttctgtaaaa aacaataaaa ttatcgttat ttctttacac gttcagcaaa
    15421 ttcatagaaa ggagccaatt tcgttaactg ttgattacat tgatcactat catgacaaat
    15481 ataattcccc tttttcgtat acgtgccatc agaaccggct ttcgttgtag aaagaaagag
    15541 cgagacattc gatgttttct gacagatcgc acagattccc ttattaatcg ttggtgataa
    15601 agtaccgctg atccctttta attttccatc ttcgtaataa accatgaatt ttttttgcgt
    15661 ccctggatca ttccaaccga tgtacgtatg tgcttgcaag tcgatttcat tccaagcggg
    15721 ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc tcggctgcat
    15781 aaacggctgg acgatttgct tcagctcctc caatgctcgt tctgcttgcg tttttgtcag
    15841 ttttacatct aaaactgtcg ttaaaaacgt atcgatttct gatggttcgt aacctaatac
    15901 atcgcgaatc ttttcagctg ctagagcctg aaccgtttgt accgtttttg agtcattgac
    15961 agatgagtac gcatttaata actgctctac ttctcttttt ataaaaaaat attgatatgg
    16021 tttcattgtt tgttccatga ttttaaagtc ctttcttcta accaaagtgc atactttgta
    16081 gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga tcccggactc ttttaaataa
    16141 gatacgtttt gcatcttatc atcaccccct ttaatgttca ttcttctatg tacatgattc
    16201 attcgataaa taaaagtaaa ccgtatcttg agcagatagt caactgcaaa cagaaaaagg
    16261 actgtgacaa aagtcgtgtc aaagtccctt tctacaaaca aacggtgttc cagaagtagc
    16321 ttcttcaaaa aataagccgg aatttcacaa aaatttgaga agcaattttc gtaaattcct
    16381 tcttatttct tgaagctgac ccacttctgt cacaacctca atatggtcta ttattggttt
    16441 tacctgttaa atcgaggatt cttttccgaa gtaacgttgc catctcttac gcatacttgc
    16501 tgacataggc tgagcggctt ctttgactgc gcagtatttg tctacgaatg ttacgatata
    16561 tccttctttg tacttaggcg gtgcaatcgt tgcgccccac atgtgcttca aaatgatatc
    16621 tcgctcaaga tccgacaaat cggtgatctt ttctgcattc tttacagcga ttcttggatg
    16681 gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc caatcatagt agaacagatc
    16741 atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag
    16801 ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt
    16861 gtaattcgct aattttttca ctgcttctgt ttctaaaaga tcttctacat aagacatata
    16921 ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt tttgaatttc
    16981 attttcttct gaaattgatc taagtatacc accagtgttc ggattattct agtcgccttg
    17041 gataaaatag ccaaagctta atagttcctt aatcataaag aaaaatgata aagaacaatc
    17101 cctgccgcaa ccccaacatt caacgattcc gcatttcctt tcatcgggat atatacgttc
    17161 tgatcagtca aagacaagat ttcctgactt accccctgac cttcgttccc catgattatt
    17221 gcgaagttat ctgatttttc tactttgtct aaggcgtgtg cttcttcatt taattctgtg
    17281 ccatacactg taaacccttt gtctttcatt aaaggaatca cttctgtcaa atctttttgc
    17341 aacacaggca aatgataatt gcttccctgc atgctgcgca gcacctttgt actataaata
    17401 tctgccgttc ctttgcctaa taaaacacca gccatatcaa atgcatctgc cgtacggatc
    17461 atcgtcccta catttccagg atcttgtaca ttatctaata acagccatcc tcctgaaaaa
    17521 tctggcgtgc cttcagccgt taacgccatc actgctaaga ttccttgtgg tgtaggtaga
    17581 tccgatacag catccatgac ctcatccgaa actagaaaag tagaaatctc tttgtttgcg
    17641 gccagccagt ctccccattc tttcatgcct cgctgtgtca caaaaagttc ttttatttct
    17701 gcatcagacg acgcagcttc ttcaatcaga tggaaccctt ccaaaagata ttcttccttt
    17761 tcttcccgat atttcttttt atgtagtttt tttaattctt tgattcttgg attttttgct
    17821 gattggatct ctttcacatt cctcacctcc ccatgattat atcatggaat aggtgcttga
    17881 gcttgtttca tcctaaaaaa atcggtatca ttaatttata aaataggaaa gaaaggtgac
    17941 gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc caaggcgtag gatttcgcta
    18001 catgaccaag atggtcgctg atcaactagg cgtgactgga acggtaaaaa acgaagatga
    18061 cggctcagtc acgatcgaag caatcggcaa tgacgacatc atccagaaat ttattgaaga
    18121 agtcaagaaa tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagaccc
    18181 gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa aagctggtct
    18241 gcatcaagca ttaaggacag gttgtgaaaa atagctctcc atatttttca acaggctgga
    18301 cttaatgccg tagatgcaag cttttgaaca ccgtcaataa ggttgtgaga aagtcgctta
    18361 gttttgagta gtaaggagaa ttttgtgaaa atggctttcc acatttttgc ataaaatttg
    18421 acttaatacc gagaaactga cttttgaaca ccgtcgataa ggttgttttt gagacgcttt
    18481 gcctcaagta ttaaagatag gtagtaaaaa agctgatcta taacttgcaa taaatttgct
    18541 taacccatat tttccagaca gaacaaaaaa gtttttagtt ttttaaataa gaaa
//
LOCUS       DASJST010000051        18511 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJST010000051 DASJST010000000
VERSION     DASJST010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.051"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..754
                     /locus_tag="U4B05_001386"
     CDS             107..754
                     /locus_tag="U4B05_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2050536.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    883..1099
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1147..3792
                     /locus_tag="U4B05_001387"
     CDS             1147..3792
                     /locus_tag="U4B05_001387"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN2050537.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4102..5415
                     /locus_tag="U4B05_001388"
     CDS             4102..5415
                     /locus_tag="U4B05_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN2050538.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5405..6058
                     /locus_tag="U4B05_001389"
     CDS             5405..6058
                     /locus_tag="U4B05_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2050539.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6113..6804
                     /gene="radC"
                     /locus_tag="U4B05_001390"
                     /pseudo
     CDS             6113..6804
                     /gene="radC"
                     /locus_tag="U4B05_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6954..7154
                     /locus_tag="U4B05_001391"
     CDS             6954..7154
                     /locus_tag="U4B05_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2050540.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7408..9087)
                     /locus_tag="U4B05_001392"
     CDS             complement(7408..9087)
                     /locus_tag="U4B05_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2050541.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9089..9301)
                     /locus_tag="U4B05_001393"
     CDS             complement(9089..9301)
                     /locus_tag="U4B05_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN2050542.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9694..11424
                     /gene="efrA"
                     /locus_tag="U4B05_001394"
     CDS             9694..11424
                     /gene="efrA"
                     /locus_tag="U4B05_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN2050543.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11421..13181
                     /gene="efrB"
                     /locus_tag="U4B05_001395"
     CDS             11421..13181
                     /gene="efrB"
                     /locus_tag="U4B05_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN2050544.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13276..13473
                     /locus_tag="U4B05_001396"
     CDS             13276..13473
                     /locus_tag="U4B05_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050545.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13626..14111
                     /locus_tag="U4B05_001397"
     CDS             13626..14111
                     /locus_tag="U4B05_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN2050546.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14215..14808)
                     /gene="clpP"
                     /locus_tag="U4B05_001398"
     CDS             complement(14215..14808)
                     /gene="clpP"
                     /locus_tag="U4B05_001398"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN2050547.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14988..15515
                     /locus_tag="U4B05_001399"
     CDS             14988..15515
                     /locus_tag="U4B05_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050548.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15606..16343
                     /locus_tag="U4B05_001400"
     CDS             15606..16343
                     /locus_tag="U4B05_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2050549.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16347..17264
                     /gene="miaA"
                     /locus_tag="U4B05_001401"
     CDS             16347..17264
                     /gene="miaA"
                     /locus_tag="U4B05_001401"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN2050550.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17266..18495
                     /gene="hflX"
                     /locus_tag="U4B05_001402"
     CDS             17266..18495
                     /gene="hflX"
                     /locus_tag="U4B05_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN2050551.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3202 c         4023 g         5302 t
ORIGIN      
        1 accgttttat ttgtctcaca cccttttcct tcctacttgt aaccttgctc ccgtgttaga
       61 aacatgttac actaaatatg tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac
      121 agatacactg ccaaaagcaa ctgcgaaaag attaccttta tatttacgct acttgaagat
      181 gctggatgac tcaggtataa gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat
      241 tccttctgca acgatccgca gagacttctc gcaattaggc gagctaggca gaagcggata
      301 tggctatgat gtcccttttc ttatcgatgt attcaataat atccttaata ctaaagaaga
      361 aaaacgaatc gcattagtag gatatggaaa tcttggcaaa gcattgaaac acaacaattt
      421 tagaagaaat gaaaatctaa atatcgtgtg cgtctttgat aatgatccag cactgatcaa
      481 tcgagtaatt gatggagaaa tgatctatcc gattgatcgt ttcgcggaga tagccaaagc
      541 aaaaaatgtg acagtagcaa tctctacggt accaagtaaa tattcacaat ctgctattga
      601 tgaaattgtc aaaggaaatg taaccgctat acttaatttt gcgcctgaca gagtaactgt
      661 cccagcttat gtcaatgttc aatacattga tttgacaacc gagttgcaga cgcttattta
      721 ctttgatgag aattactcag aagttttttc ttaggaaaat ttgagtaggc ttattgatct
      781 tgcgagaact cgtcgtaatt ctttcaaaaa ttttttagta ctaaatgaag tggttttttc
      841 acgaccactt gttgactaaa aaaaaaaacc attgtagaat gaagcataac gctgaacaag
      901 aggagtaact ggcacattca ttcttagaga gagaactatc tgctgaaagg ttctcaatga
      961 caaccagtga aggtagcttg ggagtttctg tcttgaatgg agtagagata gacgagtcgt
     1021 gatcgttaac tcacttgaga ggcaataatg attgattatt gcaatttagg tggtaccgcg
     1081 taaatcatac gtcctagaat acgaaagtat tttaggacgt tttttagttt aaaggaggaa
     1141 aacagcatga cggaagaaaa taacctatca acaaaatata atccgcaaga agttgaagct
     1201 ggacgttatc agcattggtt agaacaagat ttgtttaaac cgagcggaga taaaaaagca
     1261 aaaccttatt caatcgtaat cccgccccca aatgttactg gaaaacttca tttagggcat
     1321 gcttgggata cgaccttgca agatatgctg attcgtcaaa agagaatgca aggatatgat
     1381 actttatggc tgcctggtat ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg
     1441 aagcttagag agcaagggat ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa
     1501 gtgtgggaat ggaaagaaga gtatgcgagc cacatccgcg aacagtgggc taaaatgggc
     1561 ttatctttag attacagtag ggaacgtttt acattagatg atggattatc tgaagcagta
     1621 cgtaaagttt ttgttacctt atacgaaaaa ggtttgattt atcgtggtga atatatcatc
     1681 aactgggatc ctcaagcacg aacagctcta tctgatatcg aagttattca taaagatatc
     1741 gaaggcgcct tttatcatat gagctatgaa ctagcagatg gatcaggcgt agtagaaatt
     1801 gcgacaaccc gtccagaaac gatgttggga gatacggcga ttgctgttca tccagaagac
     1861 gagcgttatc aagcgctcat cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc
     1921 ccaatcattg cagatgaata tgtagatatg gaattcggga caggtgtagt aaaaatcact
     1981 ccagcacatg atccaaacga ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat
     2041 gttatggatg atgatggtac gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt
     2101 tttgctgctc gtaaagcaat cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc
     2161 gatacgatga tccacagtgt gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg
     2221 ctgtctactc aatggtttgt aaaaatggcg ccgttagcga aaaaagccat tgataaccaa
     2281 gaaacagatc aagcagtgga attcttccct ccaagattca atcaaacgtt tttacgttgg
     2341 atggaaaatg tccatgattg ggtaatctca cgacaattat ggtggggaca tcaaattcct
     2401 gcatggtatc acaaagaaac aggtgaaatg tatgtaggga tggaagcacc agcggatagt
     2461 gaaaactggg tacaagatcc tgatgtttta gacacttggt ttagttcagc tttgtggcct
     2521 ttctcaacaa tgggctggcc agatacggaa agcgaagatt ataagcgata cttccctact
     2581 agcactttag taaccggtta tgacatcatt ttcttctggg taagcagaat gattttccaa
     2641 agcttggaat ttactggcga acgtccattt gagaatgtct tgattcatgg attgattcgt
     2701 gacgaacaag ggcggaagat gagtaaatca ttaggtaatg gaatcgatcc gatggaagta
     2761 atcgaaaagt atggagcaga tgctctccgc tggttcttat cgaatggttc tgcaccagga
     2821 caagatgtcc gcttcagcta tgaaaaaatg gatgcttctt ggaactttat caacaaaatc
     2881 tggaatgctt cacgtttcgt tatcatgaat atcgaaggaa tgaccgtaga agagatcgat
     2941 ttatctggag aaaaatcagt ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc
     3001 gaacgagtaa ctgagctatt tgatcgtttt gaatttggtg aagcaggtcg acaactgtat
     3061 aactttatct gggatgattt ttgtgactgg tacatcgaaa tgagtaaaga agtactttat
     3121 ggagatgatg ctgtttcaaa acaaaccacc cgtagcatct tagtccatac acttgatcag
     3181 atcctacgtt tattgcatcc aatcatgcca ttcgttacag aagaaatatg ggaacatatt
     3241 cctcatcagg gaaattctct agtagtggca gaatatcctg ttgtacgtcc agagtttgat
     3301 gatgaaacag cttctaaagg gatggaagta ttgaaagaat tgattcgagc cgttcgaaat
     3361 attcgttcag aagtgaatac accattgtct aaaccaatta cgttgctaat taaaacaaac
     3421 gatgctaaaa tcgatcaatt tttagttgaa aatacaagct atatcgaacg cttctgtaat
     3481 cccgaagagt tgacgatatc aagtgagatc actgcgccag atctagcgat gtcagctgtt
     3541 ctgacaggtg cagaaatctt cttgccgtta gcaggattga tcaatattga agaagaaatc
     3601 aaacgattag aaaaagaact ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa
     3661 ctttctaatg aacgttttgt ttctaacgcg ccggaagaag tagtagaggc agagcgtgca
     3721 aaagaaaaag attatttaga gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt
     3781 actattcaat aagaatagaa aaagagagtg tgaaatcagc tagctattac taaaagctag
     3841 ctgattgaac atcgttacag gaaagagttt gagcctcact cttagcttcg attcataagg
     3901 agatgtgaca taaattttgt cgcatctctt ttttagtcta ctcttattat gtaggagaag
     3961 gagggaggtg gatcatgtat caagagtcaa taaagtagtt gataaaatcg ttcctctctc
     4021 tgtttctttt ctctttgtca tacttttatt tttcttatgt gttctatttg ttataataaa
     4081 agagaaatgg gggaattgtt gatgacgatt gaagaagcaa tagaatggat ccatagtcga
     4141 ttaccatttg gttcacgtcc tggccttgac cgagtcgaag ctttattaga agtggtaggg
     4201 catccagaga gaaaagtaaa aacgatccat atcgctggaa caaacggaaa aggttctact
     4261 gtagcttatt tgagaagcct attagaagag gctgggctga cagtaggtac atttacttca
     4321 ccttatatcg aaacgttcaa tgaacggatc gcaatcaatg gcaaaagcat tccagacaat
     4381 gatttgatcc agcttgtaaa aaaatatcag ccactgatca aacaacttga tcaatttgaa
     4441 gcagtagctg gtattactga attcgagaca ttaacagcaa tggcatttga ttattttttg
     4501 atgaagaaag tggacgtagc gattatcgaa gttggcttag gcggcttatt ggattctacc
     4561 aatgtagctg taccaatgct gacgggaata acaacgattg gacttgatca taccgaaata
     4621 cttggagata caattgaaga aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt
     4681 ccagtagtaa caggaaatat tgccccagaa gcttttactg tcatccaaca aacagctaaa
     4741 gcaaaaaatg caccgcttta tgtatacggg gaaaattata aaataaacta tctgcatcct
     4801 gatgagatgt ggggagaagt atttgaattt tttaatgaaa taggaaagat ccctcaatta
     4861 accactccgc ttttagggca acatcaagct gaaaatgcag gagtagccat cgaattgtat
     4921 tttgtttact gtcaattgga aaagctgcct ttccgagaaa aagatgtttt gaagggattg
     4981 aagaaatctc gatggccagg aagaatggag cgtttgagta acgagccatt agttgtttta
     5041 gatggtgccc ataatacgca tgcaatgaac cggcttgttg agaatttgaa aaaagaattc
     5101 aaaggatatc aaatcaacat tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg
     5161 ctagaacgat tgcagcaagt accaaatgtc catatttatt tgacgacctt tgaacatcca
     5221 aaagccatcg aattaagtga agtatcagat ttagcaagtg agagagtatc aatcgtttca
     5281 ttgtggcaat tcggattagc agatatattg gaaaagatga caaatgaaga cgtattagtt
     5341 gtgaccggtt cactttattt catttcagaa gttcgccatt tattaataca attaggagga
     5401 gatcatgaag tataatgcag tgatttttga catggatggt ttgctgttcg acacagagtt
     5461 agtgtattac gaagccacac aagtagtagc agacaagatg ggttttcctt atgataaaga
     5521 cctgtattta aaattcttag gtgtttcaga tgaagaagta tgggcgaatt atcatatgat
     5581 tttctcggaa tttggcaaaa caagtgtaca aagattcata gatgattcat accaggaaac
     5641 gcttgaacgt ttttcgcaag gagcggtaca aatgaagccc ggtgtgttgg aactcctctc
     5701 ttttttagaa gaacatcaga ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat
     5761 tgaactttta ttgaagaaga acaatctgtt tgaacgattt gagacgattg tttctgcaga
     5821 aaatgttcaa cgagcaaaac cagacccaga aattttcttg ttagctaatc agaagctagg
     5881 tacagcgaag aaagaaacat tgattttaga agattcacaa aatggtattt tagcagcttc
     5941 tgcggctgag atcccggtag tcatggtacc agatctgctt ccaccatccc aagaattgat
     6001 caaaaaaaca gctggggttg tttcttcttt acatgaaatc cctgcattgt taaaataaaa
     6061 atccatctct tttcgtatct tattttagat aagatagaaa ggagatgttt ttttgggaaa
     6121 acgattgatc aaagaagtac ctactagctc tttaccaagg gaacgtatgg aaatttatgg
     6181 cgcggaagct ttatcggatc aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc
     6241 ttatagtgtg atgtcgattg ctggaaatct gttaaaaacg tttggaggac tggcctcttt
     6301 acgacaggct actttgcatg agttagaaga gattcgaggg ataggacgcg tgaaagcaat
     6361 cgaaatcaaa gccttgatcg aattaggtcg acgaattcag acggatcatg aaagagcggc
     6421 tccaccggtt cgtaccagct acgaattggc ccagcaattg attctggaaa tgaaagatca
     6481 caaacaagaa catttggttt gtatttattt ggacacgaag aatcaagtga tcttaaaaaa
     6541 acagtgttca tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat
     6601 gctgtacgtt attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt
     6661 attccatcgc agcaggatat gaattttacc aaaaggatac aaaagtgtgg agaaatgatg
     6721 gggatcactg tactggacca cttgatcatc ggacgcaaac gatattttag tttgagagaa
     6781 gaaggaatga tggaagagaa gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca
     6841 attatattgt aattttgttt gggaatagaa gttgaaacat ctgttgcacc tctatcgtac
     6901 acgagcaatg ttacaattta aattagtgct tttggcacga ggaggatatt attatggaaa
     6961 acggaacagt aaaatggttc aacgctgaaa aaggatttgg atttatctca cgtgaagatg
     7021 gaagcgacgt attcgttcac ttctcagcta tccaaggtga cggattcaaa acattagaag
     7081 aaggtcaagc agtgactttc gacgttgaag aatcagaccg cggcccacaa gctgcaaacg
     7141 ttgtaaaagc ttaattttat ctgtaaatca aattgatggt agacatcaat ttttacgaga
     7201 gaacaaagcg agtaacaaaa atacctgcca caagagacaa tttctcttgt ggcaggtatt
     7261 tttttgtatg aaaaagtaaa taaatttaag aggctatgaa agagccacct tgtatcaagg
     7321 aataagaaca aacaaaaatg gcttctcatg tttttcattt gttttctctt atattcagga
     7381 caaggtgctg atttcataac ctcttattta ttcttcatct ggtgtcatta ccattggcaa
     7441 aatcattgga tgtctttctg tacgttcgaa taagaaaggc tgaagcgcgc cagtcatcgc
     7501 ttctttcaat ttatattctg aacagtcagg tccattcaaa cattctctta aagcgtggaa
     7561 taagattcgc tgagcttcat tgatcatttc tcctgattct cgcatgtaga caaatccacg
     7621 agagagaata tctggaccag ccaagatttc ttttttcttg atatccactg tcgcgacagc
     7681 taatacaaga ccttcttctg aaagcaggtg acggtcacgt aaaacgacat taccaatatc
     7741 accgatcccg ctaccatcga tatacacatc tccagccaca aaatgcccag caggacgcgc
     7801 agaatcgcta gttaacgcta aaacatcgcc attttccatg atgaagcagt tttctgcagg
     7861 aacacctaca tcttgtgcta gagaagcatg tactttcaac atgcggaatt ctccgtggac
     7921 aggaataaaa tattttggct tcatcaaacg aagcattaat ttttgttctt cttgtccacc
     7981 gtgaccggaa gtatggatgt tattgatttt tccgtgaatc acttctgcac cagcttcaga
     8041 cagcaaattg atcaaatgat tgacacttgt tgtattacca ggaataggtg aacttgaaaa
     8101 gatcactgta tcgtctggct ggatttggat ttgacggtga gtaccattcg cgatgcggct
     8161 caacgcggcc atcgcttctc cttgagaacc tgtacatagg atcatcgttt cattggcagg
     8221 caagcgattg atttcgtgtg catctacaaa tgtgccgtct ggaactttga tatatcctaa
     8281 acgttgaccg ttgacgatgg cattttccat gcttcgtccg aaaacagcga ttttacgacc
     8341 agttgccaca gcggcatctg ctgcttgctg caaacggaag atgttagaag caaagctggc
     8401 aaaaatgata cgtccttcga tttttgaaaa gatcttcaaa atagaatgcc caatcgtttt
     8461 ttctgattta gtaaatgttg gaatctcagc atttgtacta tcagataaaa ggcacaagac
     8521 tccttcttcc ccgatacgag ccattttatg caagtttgct ggttcgccaa caggagtgaa
     8581 atcgaatttg aagtctccag tcgctacgat atttccagaa ggtgttttca ccacgatccc
     8641 taatgcatct gggatactat gggtcgttct gaaaaagctg acagaagtct ttctaaaacg
     8701 gatgatcgta tcttcattga tctcatggag ctctgcatct cgaagcaagc catgctcatc
     8761 taatttgttt gtgatcaacg ctaatgcgag aggaccagca tagataggaa tatttgcttg
     8821 tttcagcaag tagggtattc cgccgatgtg gtcttcgtgc ccgtgagtaa tcaccaaggc
     8881 ttttactttg tgtaagtttt gtatgatgta gctgtagtcg ggaatgacat agtcaattcc
     8941 aagtaaatcg tcttccggga atttgatccc ggcatcaatc aaaatgattt catcttggaa
     9001 ttgtacccca tacgtatttt tcccgatttc acctaatcca ccaatagcaa agacgcctgt
     9061 ctcgttattt ttgatggaaa gtttcatatt aaaactccac taatgagaaa tcagcatttt
     9121 ctttttcgta ctctagatga cttccttcta gaagttgaac gaattcaata ttgtatgggg
     9181 tattttcttc cacacgttgg cgagcgtcta catcgctttt tgcctcaatg taaagagatt
     9241 ttgtttcttc ccgtttaggg ttgcgtactt ttgtttcttg ataataaact ttatagatca
     9301 ttgttttgtc tcctttatct gcgtaaagca gtgatttagc tagttgattt ttttagacaa
     9361 cacaaaacga ggggacaagc cacctggttt ttcatatgct ttgactagaa tactggattc
     9421 gagtcaaaga gacaagcctc gtttttgctt atctgttcgg atattttttc aaccgtacct
     9481 tctgtataaa aaatatacaa caattaattg ttattctagc ataaaacagc aaataagtat
     9541 agaaatcaac tagattgaaa gaaaaaatca atgtttatca tcccgctata atcataatga
     9601 cttttttatg agagaagtgt tctattaata ggctcgttgt gctatcattt ttagtaatga
     9661 aaatgtaaca aaagattaaa aggagatgag aaagtgaaac taatgtggca ttataccatg
     9721 cgttataaga aattcttgtt tttcaatttc atatgcgtat ttggttttat tttgattgaa
     9781 ttaggacttc ctacgatttt agcacgtatg atcgatgtag gaatccgcaa tgatgatttt
     9841 gattatgtga agcaacaagg gatatttatg atcgttatta ctgtaatcgg tattattttg
     9901 aatatcttgt taggttattt tggttcgagg attacgacta atatcgtagc agatattcga
     9961 gatgatttgt tcgaaaagat ccaaagttat tcgcatcagg aatacgaaaa tctaggcgta
    10021 tcttcattga tcactcgtac aacaaacgat gcttatcaga tcatgttgtt cctgcaaaat
    10081 attttacgta tcggttttat ggcaccaatg atgttcattg tcagtttgta catggtcatg
    10141 cgtacaagtg tgacactcag tttgtatgta gtaggtgcat tacctttact attattggca
    10201 gtcgtagcga ttgctaaggt gtctgaaccg ttatctaaaa agcaacaaaa aaacttagat
    10261 aaaatcaaca gtatcttgcg ggaaaatctt tcaggattgc gtgtgattcg tgcattcgtc
    10321 aatgaaaagt ttgaagaaaa acgttttgct gtagtcaatg agaattatgc ttctagttca
    10381 aaaagcttat tccgtttgat ggctgttgca caaccgggtt tttatttctt atttaatatc
    10441 gtaatggtat tgatcatctg gagcggggca gtgcaaatcg atgcaggcga gcttcgtgta
    10501 ggagatttga ttgcgtttat cgagtatata ttccatgcct tgttctcatt catgttattt
    10561 gctagtgtgt tcatgatgta tccaagagca gctgtttcag ctggacgtat ccaagaagcc
    10621 ctagatgcca aaccttcgat tgtagaagat ccaaatggta tatctgatac aaaaactaaa
    10681 gggtatatcg aatttcaaaa tgtaacattt gcttatccag gccattcgca agagccagtg
    10741 atacgaaatg tcagttttac agcctctcca ggagagaccg ttgcttttat tggaagtacc
    10801 ggtagcggaa aatctacgtt gatccaattg atccctcgct tttatgatgt gagtgaagga
    10861 aagatcttga tcgatggagt agacgtgaga gattatcggc taagcaagtt gcgagataag
    10921 attgggttta ttccgcaaaa agcgctgcta tttactggaa cgattgcaga taatttgcga
    10981 tacgggaaag aagatgctac gcaagaagaa atggaacgtg cagctgagat tgctcaagct
    11041 tcagaattca tttctcgaaa accagatgga tttaatgaac atttatcaga aggtggttcc
    11101 aatttttcag gtggacaaaa acaacgattg gcaatagcca gagccatcat acgccgtccg
    11161 gaaatctata tttttgatga cagcttttct gcattagatt atcaaacgga tgcgaagtta
    11221 cgtgctcgtc tgaaaaaaga aacgacagaa tctactgtat tgatcgtagc acaacgtgtg
    11281 ggaacgatca tgcatgcaga taagatcatc gtattaaatg aaggagaagt cgtcggtatg
    11341 gggacgcatc gggagttgtt ggaaaaatgc ccgatttatt atgatattgc cgcttctcaa
    11401 ttatcaaagg aggaattagc atgaaaaaaa ccttttcttc cttaaaacgt ttagctcgat
    11461 atatcagacc gtatcgtgag acttttcttt tagtgatcct atttacagtt ctgacagtgg
    11521 ctttcaacgt ggctttgcca tatgttacag gacttcctac tacagaaatc agccgtaata
    11581 ttgccaatgg cgaatcaatc aatttttctt atatattcaa atgtctcatt tggatcacag
    11641 ttgttggaat agggtattgt atttcgcaat tactttctgg tgttttgatg acaaatgtcg
    11701 ttcaaagtgc catgcatgat ttgagaagag acatagaaga aaaaatcaat cgtcttccgg
    11761 tttcatattt cgataaaaac cagcaaggaa atattttgtc tcgtgtgacg aatgatgtag
    11821 atgcagtcag cggtgcttta caacaagctt ttattggtat cgtaaatgcg atattgggaa
    11881 ttacgttggc tgcagcaatg atgttttata tccagccgat gatggcgctg atctctatga
    11941 tcatgattcc aacatcgatc tggatctcaa aacgagtgat caatgcttcg cagaaatatt
    12001 ttcaaagcat gcagaattct cttggtgagc taaacggcta cgtccaagaa aatatgacag
    12061 gttttagtgt attgaaagta tatggacgcg aaaaagaaac attggaagga ttcaaaaaag
    12121 ttaatcacag cttgaaatac tttgggtttc gtgcagcatt catttctggc ttgatgatgc
    12181 ctctagttca attaactgcc tatgctactt atattggtat ggctgttcta ggaagctttt
    12241 atgttattac tggggcgatc gttgtcggtc aactacaagc attcatccaa tatatttggc
    12301 aaatcagtca gccaatgggg aacgtgacac aattgtcttc agttttgcaa agtgcatcag
    12361 cggcaacgaa acgtgtattc gagattttag atgagccaga agaaaaagaa aacaaagtag
    12421 atgcttcact gccagaaacg attttaggag atgtgacatt cgatcatgtc gattttgcat
    12481 atgatccgaa gaaaccatta atccaagatt tgaattttga agtgaaagct ggtcaaaccg
    12541 tagctgtggt cggtccgacc ggtgccggca agacgacatt gatcaatcta ttgatgcgtt
    12601 tttatgacgt agacaaagga gcaataagga ttgatggaat cgacaccaaa tcaatgagtc
    12661 gcagtgatgt gcgttcgcta tttggtatgg tcttgcaaga tgcatggctt tatcaaggaa
    12721 cgattgcaga taatatccgc ttcggaaagc ttgatgcgac agattatgaa gtagttgatg
    12781 ctgcgaaaac agcaaacgta gatcatttta tccgaacgat gccagatgga tatgatatgg
    12841 ccatcaattc tgagggagac aatgtgtctt taggacaaaa acagttgctg acgattgccc
    12901 gagcagtggt ctccgatcct aaaatcttga ttttagatga agcaacaagt tcagtagaca
    12961 cccgtttaga agcgttgatt caaaaagcaa tggatcgcgt gatggaggga cggacaagtt
    13021 tcgtcatcgc tcaccgttta tcgacaatcc gtgatgcaga tctgattcta gtgatggatc
    13081 aaggtcagat cattgaaaaa ggaacgcatg agagtttatt ggcacaaggt ggattttatg
    13141 aaaaactgta taacagccaa tttgctgagg aagcagaata agtcatataa gaataaaaca
    13201 gtttaaaact tattttttgt tctctattct tgataatggt aaagtaaagt tatcaagaaa
    13261 agtgaggaca cggagatgga acagttttta aatgaaaaaa ttactgaagc caaaagagaa
    13321 gttgatcgtt taacgcacgt ctgtcagacg aagccttttg attcagctga tttttttgaa
    13381 aatgagtttc aaatcgaacg cttggcagca caaatagaag tgtacgaatt aattttaaaa
    13441 aaagtaatag aagaaaatat agatgtacaa taaaaacagg taaaacgtca gttaccagat
    13501 actgtaaata gacatctggc ctgagcaggt tttacctgtt ttttttctta tgaaataaat
    13561 atatgctatg atacctttat tcaataggat tagtttctac aaaaaatgaa aggagaaaaa
    13621 taaatatgga acggtttata gcatcaaatg ccacagtgat tggtgatgtg acattgtcag
    13681 aagatgtgac gatctggtat caagctgttt tacgcgggga cagtaattgg ataaaaatag
    13741 gacaaagaac gaatatccaa gatggtacaa tcatccatgt cgatcatgat gcacctgtag
    13801 atattgcgga gaatgtgacg gtaggacatc agtgcatgct acatggctgt acaatcgaaa
    13861 aaggggcgct gatcgggatg gggaccacga tattgaatca tgcggttatt ggtgagaata
    13921 gtttgattgg tgctggatca ttagtaacag aaggaaaagt tattcctcct aatgtcttgg
    13981 cttttggtcg cccagctaaa gtgatccgtc ctttgacaaa agaagaaata caaaaaaaca
    14041 aagaaaacat ccagcattat gtcgagatag gtcggatgca tgcgcaagga aaattcaagg
    14101 agcaaagata ggcagtagca tccataaaat aaaaaaggat ggtcagtttg agttgaccat
    14161 ccttttttat ttggtttaga taagagagct taaaaaagct tcctttgaaa cagattaatt
    14221 taatgaagaa ctgttttcca tgacttcatc gatcaatcca tatgcttttg cttgttcagc
    14281 tgtcatatag ttgtcacgat ctgtatcttt ttcaatcact tcaagcggtt gtccagtgcg
    14341 ttcagctaaa attttgttta agcgttcgcg tgtttgcaaa atatggcgtg ccgcaatttc
    14401 gatttcagtt gcttgccctt gagcgccgcc aagaggttga tggatcatga tctcagcatt
    14461 tggtaaagca aatcgtttac cttttgtacc agctgtcagc aagaagcttc ccattgaagc
    14521 agccataccc ataacaattg tttggacatc tgctttgaca aagttcattg tgtcgtaaat
    14581 agccatacct gctgtaactg aaccaccagg agaattgata taaagataga tatctttttc
    14641 agaatcttga gcgtctaaga aaagcaattg ggcgatgatc gaattagcaa gatcatctgt
    14701 tacttggccg cttaacatga tgatacggtc tttcaataaa cgtgagtaga tatcgtaagc
    14761 tctttcacct cgtgaagatt gttcaatgac tgtaggaatt aaattcataa tagagttcct
    14821 ccttgatttc gtttagaaat agtgtaccat aatttagttt ctaataccta aaatgtagta
    14881 tacatttttg gtcaaaaaag gtcaaataaa aaacttaggc aaattagtcg cataaacccg
    14941 ctttatggta tgataagcag agaaacaaaa caaggaggaa caacagattg atagttggta
    15001 ttactggcct tatcggagtg ctgacaggtg ctgtagttgt ctatatcggc atgatactgc
    15061 aaatgcgtca cttggaaaat aaagaacagc gtagacgtga attggaattg aaagtcaaag
    15121 aaatcgagac gctgaatcaa ttaaataaaa agatcaacga gatcttgcaa aaacggacaa
    15181 cgctgatgga agaatatgtc agctttgatg catttgatga ttgttatatc acgattgacg
    15241 attttgcgta tctccaatca ttttcagcgc aaaatagttt ttatctgcca aattatattt
    15301 tagacgagct atttaagaat atctcccatc gaaaagtgat tttatctcca gatgaaacaa
    15361 tgaagattgg tggatatgct tataaaggcg gacgcgtgat tttagagaat tttacggata
    15421 gtctgactga aatgatcaat gaaaaaaaag ctcagttgaa gactatgacg cgccagccga
    15481 ttggtttctt ctcgaaagat gatgtttgga gctgatattt tgaagcattg ccgttgcaca
    15541 tgagagcgtg gtaatgcttt ctttttttct taatgataca atagaactga acggagtgaa
    15601 gaaagatgtt acaaatctat gcccatcgcg gaagcagcgg cacccaccca gaaaatacgc
    15661 ttcctgcatt tgcagaagct gtacgagtag gtgcagatgg tatcgaacta gatgtccatc
    15721 taagcaaaga tggttatttg atcgttatgc acgacgaaga agtggaccgg acgactaacg
    15781 gtaaagggct gattcgagaa aaaacattgg aagaactcaa aaaattgaat gcagggggca
    15841 aattcagcaa agaatttcca gcagctaaag ttcctgaatt gagagaagtg ttaggactgc
    15901 tcctccaaaa aaattaccga gggatgctaa tgattgaaat taaaactgat cagtatgaat
    15961 atcccggtat cgaagaaaaa ttagccgaaa cattaaaaag tagagattgg ccttttcaac
    16021 atgcatattg cagctttcat ttggattctt tggaaaaaat cgcattactg gaaccggaga
    16081 tacagctgga cttattgatg agtacttctg ataaaaagat ccaactagca gcagaaaccc
    16141 cttatataga aggagtacat ccaaaaaata cttggttgtt cgaacatcca gtccaggcag
    16201 ctgattttcc aaaagcaatt cgtgcatgga cgattaacga tgaagaagaa atggccaaat
    16261 gtcaagaact caaaatagat ggaataataa ctgattttcc ggagaaagct cttcggtgga
    16321 aacaatcaac gaaaagtagg taaaatatgg aaaaagtatt agtgatcgtc ggtccgacag
    16381 cagtcggtaa aacagcgcta agtgtggaac tcgcacaact atttggagga gagatcatca
    16441 gtggtgattc attgcaaatc tataaaaatc tggatatcgg tacagcaaaa atcaaagaag
    16501 aagagatgaa taatgttccg catcatttga ttgatgtgat cgaacctact caacgttact
    16561 cagccgctga ttttcagcat tcaggtcgac aattgattac cgagattgcg aaccgagggc
    16621 acctacctat catagctggt ggtacgggtc tctatatcca atcccttcta tatgattatc
    16681 agttaggagc gacagaagaa gcgaatcctg agattagaaa aaagtatgaa acgttagctg
    16741 aaacaattgg ttcccaagca ttatggaatc agttgaaaga aaaagacccg gcagctgctg
    16801 agaaaatcca ctggaacaat caacgcaaag tgataagagc tctggaagtt ttagagacga
    16861 caggacatag tatcacggca cctaaagaag agccaaagca attgtatgat tacctcatga
    16921 ttggattaga tacgaagcgt agtattcttt atgaacgaat caatcaaaga gtcgatttga
    16981 tgctagcaga aggtttagta gcagaagcgc gtaaggtgta tgagcttgga gaagtccagg
    17041 ccagtcaagg tattggatat aaagaatttt tcccttattt tgaagggaaa gaaacattgg
    17101 aagaagcaac agaacaagtc aaactacatt cgagaagata cgcgaaacgt cagttgacgt
    17161 ggtttcgtaa ccgtttggat gcacagtggt ttgatcttat aagtgagcca aatcaaaaaa
    17221 gtcagattga gcaaacaatc aaaaaatggc tggaggcgga ataaaatgga agaaaaagtg
    17281 atcatcgtag gggtagaaac cgaagcaaac cagcgttact tttcagagtc aatggaagag
    17341 ctagttcagc tgacgaatac cgcatcagga gaagtcgtat tcacaattac acaaaagaga
    17401 ccacaagtgg atcggcaaac gattatcggt aaagggaaat tacaagaatt agttcaacaa
    17461 gcggatgcgc atgaagcgga tttgatcatc tttaatcatg agctaactcc gagacagagc
    17521 caactgatta ccgatgctgt tggtcttccg gtaattgatc gcgtgcagct tatcttagat
    17581 atttttgcga tgcgtgctcg ttcgaaagaa gggaagctgc aggttgaact tgctcagtta
    17641 gaatacctgc tgcctcgttt agtagggcaa gggaaaacat tatctcgtct tggcggtggg
    17701 atcggaacaa gagggccagg tgaaacaaag ttagaaacag accgccgaca tatacggaat
    17761 aaaatttttg ctgttaagaa agaattgaaa gaagtagaag ctcatcgaga gcgcaatcgc
    17821 caaaaaagaa aaaatagcga gatttttcag attggactta ttggctacac gaatgccggg
    17881 aagtcaacta tcttgaatct attgacacaa gcggatactt attcgaaaga tcaattgttt
    17941 gcaacgttag atccattgac taagagatgg cgttttgctg aaggatttga gattactgta
    18001 acggataccg tcgggttcat ccaagacctg cctacgcaac tgatcgatgc tttccattct
    18061 acattagaag aaagtcaaaa tatggatctt ttgctacatg tagtcgatgc tagttctcca
    18121 gatcgtattt tacaggagca gactgtctta aaattgatgg atgaactgaa tatgaaagaa
    18181 atgccgatac tgactgttta caataaggca gaccagatcg acccagcgat gttcacgcct
    18241 acgctattcc caaatgtatt gatttctact cagacgaaag aaggtaagat ggcgttgatc
    18301 gaagcaatca aacgtcagct gatggaacta atgacccctt atacactttc tgtgccaagc
    18361 aacgaaggac agaaattaag cgagttgaga agacaaacaa tggttttgga cgaagcgtac
    18421 atggaagaaa caaacgaata tgaagttaaa gggtttgcaa aaaccaattc aaaatggtta
    18481 agaaaaaacc aataaaattt tattttttta a
//
LOCUS       DASJST010000052        18080 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJST010000052 DASJST010000000
VERSION     DASJST010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.052"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..134)
                     /locus_tag="U4B05_001403"
                     /pseudo
     CDS             complement(1..134)
                     /locus_tag="U4B05_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(176..1302)
                     /locus_tag="U4B05_001404"
     CDS             complement(join(176..1074,1074..1302))
                     /locus_tag="U4B05_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2050552.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1360..1734)
                     /locus_tag="U4B05_001405"
     CDS             complement(1360..1734)
                     /locus_tag="U4B05_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2050553.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYGVSIKS"
     gene            complement(1982..2818)
                     /locus_tag="U4B05_001406"
     CDS             complement(1982..2818)
                     /locus_tag="U4B05_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN2050554.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(2829..3758)
                     /locus_tag="U4B05_001407"
     CDS             complement(2829..3758)
                     /locus_tag="U4B05_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN2050555.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            3950..4303
                     /locus_tag="U4B05_001408"
     CDS             3950..4303
                     /locus_tag="U4B05_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2050556.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(4377..5033)
                     /locus_tag="U4B05_001409"
     CDS             complement(4377..5033)
                     /locus_tag="U4B05_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2050557.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            5259..7193
                     /locus_tag="U4B05_001410"
     CDS             5259..7193
                     /locus_tag="U4B05_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2050558.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(7279..8502)
                     /locus_tag="U4B05_001411"
     CDS             complement(7279..8502)
                     /locus_tag="U4B05_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2050559.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(8496..10394)
                     /locus_tag="U4B05_001412"
     CDS             complement(8496..10394)
                     /locus_tag="U4B05_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN2050560.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(10388..11506)
                     /locus_tag="U4B05_001413"
     CDS             complement(10388..11506)
                     /locus_tag="U4B05_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2050561.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(11589..12422)
                     /locus_tag="U4B05_001414"
     CDS             complement(11589..12422)
                     /locus_tag="U4B05_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2050562.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(12415..13332)
                     /locus_tag="U4B05_001415"
     CDS             complement(12415..13332)
                     /locus_tag="U4B05_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2050563.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(13559..14683)
                     /locus_tag="U4B05_001416"
     CDS             complement(13559..14683)
                     /locus_tag="U4B05_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2050564.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            14866..15774
                     /locus_tag="U4B05_001417"
     CDS             14866..15774
                     /locus_tag="U4B05_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2050565.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            15915..17528
                     /locus_tag="U4B05_001418"
     CDS             15915..17528
                     /locus_tag="U4B05_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2050566.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5681 a         3509 c         2996 g         5894 t
ORIGIN      
        1 gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc ccaacgaatg
       61 atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta cagaagagtt
      121 cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga gacggttaag
      181 ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga atcgagctat
      241 ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga tttgcttctt
      301 caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca tggaaagatt
      361 cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt attttgttgg
      421 ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca gtgtgtagaa
      481 tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga atgacacaat
      541 ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt aaatccatga
      601 ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg tcagctgccc
      661 atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct tttgaaatga
      721 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      781 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      841 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      901 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      961 agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa tccttacacc
     1021 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
     1081 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
     1141 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
     1201 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
     1261 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
     1321 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
     1381 ataagtgaat tcttttatca aataaccact aggcgtaatc tttcgattaa taacagttat
     1441 tgctttattc ggatgagacg ttagataaac aattttacac ttttcattct tttctctaag
     1501 catttcgcct agttcgatac cgctataata ggcgttcaaa tcgatgtcta ggaaaaaaat
     1561 atctgaggaa tagatttcat tatttttaat gaattcaaca aattgtataa cgttcaatcc
     1621 ctgaacgatc tcaaatttcc aaggacttaa taaaattctg gattcttcca aaatttctac
     1681 tagcattttt tcaaaaacca aactatcatc caaaataaat atacgcgtta ccataaaaag
     1741 tacctccact cgatatttta ctatcaaaat catttcatac aacttttcaa catacatgtt
     1801 aggaataaat tgtaattctc ttcacaatct tttccaacac ctttgaaacc gatatcaaat
     1861 actttcagga tgctgcttct aaaataaata tcaaacaaat gaatacatca tgcaaaaaaa
     1921 gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct atgtttattt attttgttct
     1981 tttaaaaaga acgatacaca tactgatttt tcggagcaga gactttctca gcgtttgtaa
     2041 caactacaga tgggatttca cgcttgaatg gttgataata gtctgattgg atggttcctt
     2101 ctattttata ccattcatca tttttaaccg ttactccttc cggcatatgc gtcaacaaac
     2161 caaagactcc tgaatctgcc acacaatgga taatgccgaa tcggaacaag aagatatctg
     2221 tattgctatc gttcggtgtt tggtaaacaa atccttcata gctgattttt ttgccaatga
     2281 attcgctagg gtaattataa attagctcca taacttctaa atagttttca tctgtgatct
     2341 tgatagtttg cttatcccca tacttttctt tcaactcatt catttggtct aagtagtctg
     2401 atttgttgaa ataaatactg gtatccggct ttaagtattg tgtctgcatc tccggatccc
     2461 ctactgattc tttacttact gggaaattaa atcctttggc ttctacgatc gtagtgtcga
     2521 gactaactgt tggaaacaac gtccccacga ttactggcaa agctagcagt acataagcga
     2581 ttccacgttg ataaggtttt gacaacccat ggtcatgatc gtgctcgtgt gccttttctt
     2641 ctgcttttcc gtttttgacc cacaaataca gctgaactaa agccaaaaga gcagataaaa
     2701 tcattgaaag aatagctaag tagcgataat ggacattgat atattgatcg agtttgccag
     2761 aaacttgcaa gtacatcatc aattcgaaat acccagataa aatcaaaaac cgcagcatat
     2821 tttccctcct aaataaataa tgtgaatcca ataacgctaa gggcaatcag tccgatcagc
     2881 gataaaataa agcgtcctgt aaaatatcgt ttcatcatca gtagattttt gatatcgacc
     2941 attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc cgaacagact taacaaagaa
     3001 gatcctataa acgcatctgc ttctgagcaa agagacatcg taaaggccaa taacagcatg
     3061 atgataatgg ctaaaatctt tgttgagccg agcgttagca taattctagt tggtaaataa
     3121 gtctgcatcg cagctgcgat taatccgcca atgatcaaat aacgacctgt atcaaaaaat
     3181 tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga tcagctgatt ttttggttca
     3241 ttcttctcac tttgttcttc ttcatggaca tgatgatggt gattttctgc tactttagac
     3301 gtgatttttt ctttcaatac agcgtctttt tgaaaatagg caagccagac accaatcaca
     3361 agggcaacaa aaaagctgcc tatcacccgc cagcttgcca tctgccaaga attgccaaat
     3421 gcgataaaag tggaaaaaat cacgattgga ttgatgatcg gagcagtcag cataaaagca
     3481 aacgctgtgt gaactggtac gcctttttta acaaattgat gaacgatcgg tacgatgccg
     3541 cattcacatg aaggaaagaa aaatcctaag atcgaaccag taaaaataga gaagatacga
     3601 ttttcaggaa gccatctctt cacacgttct ggggtcaaaa atacttgtaa ggcgcctgag
     3661 ataagacagc ctaataatac gaaaggtaat gcttcaataa cgatcgataa gaaaatcgta
     3721 ctcatctgca aaaccgaatt tggaataaaa gaaaacatga gtttcctcct ttttatttct
     3781 agttcattcg ctttgcaaat atacgctttt attcaaaaaa aacaagttgc tctgtaaatt
     3841 tttacacaac tcttattttt taccaaagca cttcaattga ctttttctgt tattcccatt
     3901 aaattaaggt ctcatacata tcacttctat cataaaaggg agaataaaaa tgaaacaaac
     3961 cactaacttg actgaagttc aagacatact gcaacagtca tccgttgccg tcatttatct
     4021 ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca cgactagagg aacttctcac
     4081 acattatgat attccggcac ttcatttgga tgcatttgaa acaccagaag tagctagtaa
     4141 atttgaagtg ctgactgcgc cagttgtcct gatttttcat caaggaaaag aagtctttcg
     4201 gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac tgacagcaga
     4261 agatgattcc ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt ccaaacagaa
     4321 aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt caatcgcttt ttatttttat
     4381 tctgctgtat tttcggaatg cgcatttgcg tcagaatgca agaaatagat caaggtttgc
     4441 aattcattcg tcaaatcaac attttggatc aatacatctg acggagcatt caatcgagca
     4501 gcggtaaagt tcaagattcc tttgattcct gcttccgtta actgattgac tacttcttgt
     4561 gctctttcag cgggtatcgt taaaattgct acttcgattt gttggatctt gatttgttct
     4621 gacatatctt ccattggata aacggggata ccgtctacga tgcgcccaac gatttcctca
     4681 ttcacatcaa aagcacaact gatacgtatg ctattacttt gatggaattt gtattttagt
     4741 aaagcacttc ccaagttccc tacaccgatc agcgcaacat ttgtcaattg atcgtcgttt
     4801 aatgtctttg caaaaaagtg cataagatct tctacatcat aaccataccc acgttttcct
     4861 aattcgccaa aataagaaaa atctcgacgg atcgttgcac tatctacttg aacggcttca
     4921 cttaattcag ttgaagacac ttttttcttt ccagcattat ttaaaatcct taaatagcga
     4981 taatacaaag gcagtctttt agctgtcgcc tttggaataa tatgatcttt cacgaaatga
     5041 cctccacgta ttgtctaatt acatctttga tttctccatc atagcagtat ttataggaaa
     5101 aaatcaattc cattgctcag atattgtgaa attttattct ttattcattt atttatcatt
     5161 tctgcaaatc tcgcagtata atggaattta tggtagacta ctaattgaaa aatagttttt
     5221 tatttgagga gaattcacaa agtagaaaag aggattccat gatattatta caagccaatc
     5281 agattgctcg ccattttggt gcagaagtct tatttgaaaa catacattta gaaatcagta
     5341 caggcgctcg tgtcgcttta gttggcagaa atggcgctgg aaaatcgact ttattaaaga
     5401 tcattgctgg aattgaagca cctgatgaag gaaagatcgc taaaaataaa acagcgactc
     5461 tcggatacct cgctcaggac acaggacttg attccaatga aacagtttgg aacgagatgc
     5521 tgaaagcttt tgaacaagtt agacaaatgg aagagcggat gcgcgagcta gagattttca
     5581 ttagcgaagg tacgccagac acacctgctt atagttctta tttaaaagaa tacgaccggc
     5641 tgcaacacga tttttcagat cgaaacggct atggttatga aaatgaaatt cgttccgtat
     5701 tgcatgggtt taaattcgat tcttcatttt atgatcagaa aatcgatact ttatcaggcg
     5761 gacagcgcac acgtttagct ttagctcgca tgcttttgca acaccctgac attttgattc
     5821 tagatgaacc aacgaaccat ttagatatcg agacactttc ctggcttgag aattatctgc
     5881 aaagctatac tggtgcatta ttgattgttt cccatgaccg ttatttttta gataaagtag
     5941 tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt aactattcga
     6001 aatatctaga tttgaaagca gaacagttag caagtgaatg gaaggcttat gaaaagcagc
     6061 aaacagaaat ccataaatta gaagacttcg ttgctcggaa ccttgttcgc gcctctacca
     6121 caaaacgagc gcaaagccgc agaaagacat tagaaaaaat ggaccggctg gatcgtcctc
     6181 aaggggatga aaaacaagca aactttctat tcagtataga aaagccttct ggaaatgtag
     6241 tcttgcaaat cgaagatgcc gctatcggat atgataatgt caccttatct gaaccaatca
     6301 atctagatat ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt atcgggaaat
     6361 cgacgttgct gaaatcaatc attcaccaga tcccttttat ccgtggaaaa gaaacgctag
     6421 ggacaaatgt caccatcggc tattacgatc aaggtcaagc tgatctgcat acaaacaaga
     6481 cgatcctagc agaattgtgg gatgagcatc ctaccactcc agaaaaagag atccgaagtg
     6541 ctttaggtgc tttcttattt tctggcgaag atgtagaaaa accaatccct ctgctaagtg
     6601 gcggtgaaaa agcacgggtt gctttagcca agttagcgat gaacaaagaa aattttttga
     6661 ttttggatga accaacaaac catttagata ttgacaacaa agaagtatta gaaaatgcat
     6721 tgatcgatta tgacggcaca ctgctttttg tttcccatga ccgatatttc atcaatcgga
     6781 tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta ggagactatg
     6841 attattattt agagaaaaag caggaagaag aagaaatcgc cgaattgttg gcacaagatg
     6901 agccagaatc tgctcctgct cccacgaaca aatctgatta ttaccaaagc aaagaacagc
     6961 aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt tgaagaagaa atggcgcgga
     7021 tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc gccagaaaac ctggaagatc
     7081 acgtaaaact caatgagctg aaccaagatt tagaagctgc tagacaagat caagaaacaa
     7141 aattgacgga atgggaagaa ctaagcttgg aattagaaga atatgaaaat taacgaaaaa
     7201 accaaaacag aaaccattca tgctgttttg gtttttcttt ttttatttta tcaaacaaaa
     7261 attccatatt tacctttttc accaataaga agaccaatta ctagtcattc tcactaaagc
     7321 ttctaaataa tagtaatcac cccaaataca aggtgtattt accccattat ttttgggctt
     7381 attataaaca gactgtgtta aaaagccatc ttcttcttcc gttgtagcat atctttcttt
     7441 caattgtcgc ataattttcc aaacagcaga ctggtataag ttcttttctg ttttgtctaa
     7501 tagacctgat tctattaatt ctaataatcc acaaaccatg atcaccatac tagatgtatc
     7561 tttttcttct ctgctagaat catcaaagat caaatcccaa taacaaatgg tatcttctgg
     7621 caaccgattc ataaaataat tggctaatat tttgcttaaa tcaaaatatt tttgttcttt
     7681 caaataacga taacttaatg caaaaccgta aatcccccat gcttggcctc tagcccaaca
     7741 ggaattatca ctataacctt gatgcgtact tccatatctt ggttttccat ctttgacatc
     7801 catataaaat gtatgataag ttgaaccatc ttctcttact aaataatctg ctgcattttg
     7861 aatatgagcc tgagcagctt ttttatattt ttgatttcca gtttcttcac tcgcccaata
     7921 caataatgga agattcatgc aacaatccat gatcattcta ccttgttgtg aaggatcact
     7981 aggatctccc caagcttgta taactcctgc cttctcccaa aaacgctgaa gtaatgtttc
     8041 tgcagcatca attgaagctt ccttcatttt tcgatttccc gttattttat aatctgctac
     8101 ggttgataaa gaatacaaaa aaccaagatc gtgatgatta gtgataattt gacagtcaat
     8161 cctgtggata aaatctatat ctgctttttc agctgtttgt tgaaataaag gatcacccgt
     8221 cttctcaaat aacaaccaat taattcctgt ccaaaatcct tctgtccaac caatattgtc
     8281 tcttaatgga taacgtcctt ttataggaca ctcgcttggg aattgatact gactgttact
     8341 gatacgtttg attaacctct ccgtgtgtct gcgacaacgc tcgatctcat tattccattc
     8401 tttttccatt aattctttat aacattcatt tatccatttt tctggatgta ttatctcttc
     8461 aaacatcact ttatcaagca tttccccacc agcctttagc ataattttag tcttttttta
     8521 ttgtttttat tttggatagt tagcccggtt gtcccatcaa aatcaatttt gggacgattg
     8581 ttccatactt ctaaactata atcccgatct tgatgagcaa ccactcctgt tccgagccaa
     8641 tgggttcctt ttccaaatgt accctctaat ccaggaataa aagtgttttc ccatgtcatc
     8701 aagtttgtat tgggcaaacc cataatagct gtggattttc gtttaatagt atcgtcactt
     8761 aaacagataa tacctgaaaa gccagcttca ctcaccctat agttttcctt gtcttcttct
     8821 atgattattc cttctttctc aggagaacat tcaatagcaa accctgtctc atatagaaca
     8881 tattctgtat ctaattctac tttgtgtacg cgaatctgcc agccttcaaa aggaataagc
     8941 caagttctga tttttgcctg taaagtaggt tcccatgtag aggcaagtac ctctttctta
     9001 catacaatgt ctcgtacatt atttcgaacg atgaactgtc cataggtttt gatgctctca
     9061 ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa tccctagcat tgaatccatt
     9121 gccatttctg ctttcaattg attcgctcgc ggtatcgaaa aaccaaattg actagaataa
     9181 gcaaatttta aatatttttc attggtcaat gtatggaaat ttggccctaa ttgtcccgca
     9241 ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca aattggcttc tgaaagaagt
     9301 ttagttcctt cacgaatagg cataggcatt tctttagccg tccaatactc atggtcttca
     9361 ggtagcgcca acaataaaaa aatcttattc agccataatg gagataacgt actattatag
     9421 ggttcagtca atgtcaattg agggtaggta taaccaatat tcaggatacc tttttcatca
     9481 aatatttctt tttgcatcca ccatcttata ttccgattga ctaacccctt gatttcacct
     9541 aatttaaaag gcaaaagatc ggcccacaac atagctgacc aaaagcttga aactgcaaaa
     9601 cgataaatca tgcttcgacc aaaaggcaca tttgcaccat cttccgcaaa aaaatgaata
     9661 tagtctctag aaaaatcaat agctcggttc ttatatatct ggctaatggg cttttctggt
     9721 tctagtacgc tataaatcaa accataataa tgaaatgcaa aaggattata ataatcttgg
     9781 cgacctcttg aactgtctcg ataccagcca ttttgaagat aacaattttc tatattatca
     9841 aatgcttctt tcagtgctga ttctttaact ggataaccaa atttttggat aattgttccg
     9901 acaattatct taaaaaattg ccaattacca tctgcacatt catattcaaa aatttgcgag
     9961 aaccaatgaa taatctggct tttttctcct gtatctaatt ttttccataa taattgttca
    10021 tgatgatata acgctaataa cattgcgggc atttctacca tacgctggtc tctttcatgt
    10081 atttctcccc aataatcggg atgggaagga tctgttccag cttttattcc tgatagaatt
    10141 aatttcaacc atcgttcatc taattggtca attgcgggcc ccgctcccca taaaacccga
    10201 gagaatcctt ccattcctgc aattttattt ccatatccta ccccttgatg tatataatca
    10261 atttgtgcgc cacttgctcg ataaaacggc tggagtggtt gatttatctt tttaaataag
    10321 tcaaagtaat cctgtctggt ccttaatagt ggtactggat gtttcattgt attttctcct
    10381 ctttttacta ccaaatagct aattccgact gttctaatcg aagtaagcct tcaataaaaa
    10441 aataatcaga atagatcagt ggcacattat aagtcagttc tctatggtat gcttctgctc
    10501 cgcctccaat gatcccatct tcatccatat tccaatttgc atagtgcttt tctgtctttt
    10561 ggagtatatt gatagcagct tcatgataca tttttgatcg tacagaggaa acatatcctg
    10621 ctaattccag aagaccgcat gcagcgcata ctccagcact ggtatcaata ttcgaatctt
    10681 ttacagcaaa atcaatgaga ggaatataac cagttttttc gattttctga ataaaattta
    10741 ctgctatctt ttctgctata gctagatagt acggattttt tgtgtaacga taacttaacg
    10801 cgaatccata taaagcccat gcatttcctc tgctccaaca agaattctca ttgtatcctt
    10861 gcccgccaag taaccgaata tatttccctg attctgcatc aaattctgcg atatgcccgg
    10921 ttgatccatc tttcctcact agatacttag ctgttgtatc tgcatgctgc cgagcaattt
    10981 tagagaacct cggatcacca gtttccctcg aagcccaata cagtaatggt aaattcatca
    11041 tactatcaat aatcatccaa ccaggtttat ggtcattcca tgctactaaa tagttccctg
    11101 aaatattata gcgtccagca agtaccgtag ctgcatgtaa ccctagttgt cgactatgtt
    11161 tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa gaataaaaaa cctacatcat
    11221 gatgaacgtc tgaaaaatgg tacagggcag attctaataa ttgttcttgt tgtattgctg
    11281 cctctttgaa cttttctcgt tttgtcttgt gaaagagctg ccataaaata ccactccaaa
    11341 aaccattggt ccaccagtcg attcctctgt cctttattac gtctgtatat tttccgttgt
    11401 aagaaatata tggaatttta ttccccattc tgtctaattc cgcatctatc ttttcagaag
    11461 ctacattcaa aatttttctt gaccattcct tcgtctgttc attcatggat atcccgccat
    11521 tcttttaatt tttatgaatg tggagttgct cctagaacga actagatggt taacattcgt
    11581 tatttttttt acttatttgc agctccaatg tcattctcct taaatttaga ctgcgcccac
    11641 acataaatca agaccattgg gatcactgaa gttgctaaaa ttagcataat tgcgccataa
    11701 ttaattggct cacctgtact agaaaccgtc atatttttga taattaaggg aaccgtatat
    11761 ttatctttat ctgaaagcat gactaatgga aatagatatt gattccaact tcccataaag
    11821 ataagtaaac cactagtaat aatcgtaggt gataaaatcg gtacagcgat tttcgtaaaa
    11881 atcacccact cattggcacc atcgattctg gcagcctcca tcagtgctgt aggaaattga
    11941 ctgctatatt gtcgcatcat aaacactgaa gttgtactgg caatcgaagg gatgatcaca
    12001 cctgcataag tattcgataa tcctaaacga ctcataatgt aaaataaagg aatcaatgtg
    12061 gtaaaaaaag gcaccatcct agatagcata actgtattga acaacagttt attccccata
    12121 aaacgatact ttgctaatgc ataacccgcc attgtagaac tggctgtagc taaaactgta
    12181 ctgattacag caataacaat cgaattccaa ataatcaagg gataattata tctcgccatg
    12241 atggcttgcc agttttttaa tagcatatcc ccaaacttga aattgattcc gccacttaaa
    12301 atttcttgat tactttgcgt tgaagctaca atcatatata aatatggcat caaaaatatt
    12361 aaggcaccaa acacaataat gacgtacgta aaaattctgc tttgtatctt ttgtttagtc
    12421 atttttatca cctccgaatt taaattgaat agccgataat ataacagata caaacaccat
    12481 cgtccaggca atagcagagg cttttccaaa atcaaccatt tcaaaacttg ttttatataa
    12541 attgactcct acgaccaaag ctgaattttc tggtccattg gaagtcccaa agaatatatt
    12601 tgggatattg aaattaccca agccattgat agtggctaaa actgtggcca ttaaaatgat
    12661 tggctttagc atggggatcg ttaataaaaa gaattgccga aatttcgatg ctccatctat
    12721 ttcagcactt tcgtatatct ctttagaaat cccttgaaga ccagctaaaa ataaaatagt
    12781 gttatatcct acactaagcc aaatcatcgt gatgatcatt gatattcgta tccaaaaagg
    12841 atttgttaac cactgtacac cgttaattcc tagcgcattc aaaaatccat tgaataaccc
    12901 agtcggattg aaaaaagtca tgaatacgga agctacagcc accgttgatg taatggttgg
    12961 aatataatag acagttctaa aaaatccttt tccttttaac tgtgctgaat tcaataaaac
    13021 agcaaagtaa atagctaata gcatcgttat tggaattgat cctgccatga taatcaaaac
    13081 attcgtgtaa gatttaataa acaatggatc tcggaagata gctagataat tgtcaaatcc
    13141 aacaaattca aatgagtagc caattttcat aaaactaaaa gcaaacgaga taatgatggg
    13201 aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg accaatccat aagcactttt
    13261 tttatgagac gtatctgttg cttttttctt gctgttcttt attgtcttgc ttggtaacga
    13321 agattgcatc atcgttaagt cctcctaata ttttttaatc accagttaat tttacgccgt
    13381 acttatgagc aaaattttct gcttctttta gatatgaatt ttcaaaatca cctgtttgcc
    13441 aatagttata tgcagcggta gtcaaataag acgtagcatc tgaataagtg gctggtaaaa
    13501 cagtatttga tgtattttca ttgattttat tcaatagttc atagtatttt tgtcctcctt
    13561 agtagtcaaa ctttttctca gctgcttctg tttgataggc caatgagtta gatgtagcca
    13621 cgcccagctc cagtgcgttg gctaagcatt cttcatctgt cagcataaat gtaagaaatt
    13681 gttgtgccac ttgtggattt tttgcatctt tagggacata aaaacttgat cctcctgata
    13741 ctgcaccttt tcctggattt tcttttgaaa aaggaatcag tggagccatg cgccatttgt
    13801 cagatgcatc aggaaaattc aaagacatat tcgtagcata ccagccgcca gcaacaaaca
    13861 ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc gtagaaatgt acaatatctg
    13921 agtcgatcat ttctttgatt aactttgcag catgtttcgc ttctttagta ttcagacgga
    13981 tatttccttc ttcatcaagc aaagattgat tctgctgggc tagcatgttg ataaacaatt
    14041 cttggtcccc agattctggc aaggcaattc caaaactacc agttttttct tttaatgctt
    14101 ttgccacctg gatatattct tcccaagaac tgatttcttc aaaatctaca cctatttcag
    14161 caaatttttt tggttgataa taacaaagtg ctggggtaaa atcactagcc caaggaataa
    14221 tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt ttgatcataa aactgatcgc
    14281 catatttgtc ataaaatcca tattcatcag cactataaaa agcatcttga aatttttcca
    14341 gcacacctgc tgctgaagtg tctgccatgg agataatatc cggcatatct tcgtgtgtta
    14401 ccaattttgg ggtgatcatt tgtactgttg ctgttcccga agctacattc acaattttta
    14461 agtcaatatt tgctccagtt ttgcggttat agatttttgc agcattttca acatagtttg
    14521 tcagtttgcc gtttgcgaca tatacttcta atgtagtggc tttttttatc attggatctg
    14581 cgatgtttgc tgttacttct tcgttagttt ttccacatgc acttaagctg aataataggg
    14641 taatgtttag caaagctatc accaatttgt atcttttttt catgtctatc ccttctttct
    14701 tttcaagttc ttatataaaa cgctttcact tatgtatatt aacggaatta gtaacacaaa
    14761 tatttatata aaaaaatatt ttacttgtcg ttttggcttt tcatttttgt tataatagtc
    14821 attaattata aatagaaagc tttttatata aaaagagaaa ggaaagtggt tcttttgtta
    14881 acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa cctattccat agatacccat
    14941 cttcatttat ctgctgctcc ctattttcga ctactaataa atgaaagcgc taatttatat
    15001 gctgaatttg cacataaatc tatagaacta gataatcctt cccttttatt cattgataaa
    15061 cagcaagaag tccaaatctt ttctactcaa aaggaaacac aaacttttat cattatccag
    15121 ttttattttg atgagcaatt attagcacat attacgggac aatctttttc tatagatcaa
    15181 gaaataacat ctatcattac ttttctgaaa aatgaacagt tcaaaattga ttatctaaaa
    15241 catagtaagc cagctatttc ctatgatcag gaaatcaatt cttttttcat actatgccaa
    15301 cagatgagca ttcatttgct ttccttatta gttacttatt gtctgcaaaa aattcaattg
    15361 tcttccttac caaggcatta tcaacaggaa cttgctttat tgacaatacc ttcaaatgag
    15421 aaagtcattc ctaaaaaaaa gcatattctt tattcagatg aattagttga tccaatcata
    15481 cgatatttag aagaacatct ttcagaaaat tttttgcttg aacaattagc ccaaattttt
    15541 tttatcagtc cagccagctt aaaaaaacat tttagaaaag tgaatggaaa aagtgttatt
    15601 agctatttta aagaactaaa aatggaaaaa gcaaaacgtc tcgtgctaga aaatgaatta
    15661 acctatacag aaattgcaga tatattagga tttcattcga tccatcattt ttcttcagct
    15721 tttaaaaaat atacagggtt atccccttca aagtattatc tatccttaaa atagaaaaaa
    15781 atttttaggt tttatccaca aattatttct ctatattttc tgtctattca aaataaacaa
    15841 taattttccg gcacaattat ttgctgtttt ttaccataac atagtacgat caacgtagaa
    15901 aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt agctggcggt ccttcatttg
    15961 ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat tatctatgaa cgcggagaaa
    16021 atatttctta tgcaagttgt gctttgcctt attatttagg aggtgtgatc actgaccgtg
    16081 actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa aaacaacatc gatgtcttta
    16141 ctaaacacga agtaacagca atcgatcctt ctactaagcg attaacagtt aaagacctat
    16201 ccacaaatga agaaataaaa acagattacg ataagttgat catctcttct ggtgctagac
    16261 cagattatcc ggatattccc ggagtttttg aagcagaaaa cggctttgtc ctccgtagtg
    16321 tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa aaatccacaa catgtcgtca
    16381 ttcttggtgc aggtgttatg ggtctggaat tagctgagaa tttcaagcat cgcggcttag
    16441 acgtgacttt aatcgatcaa ttgccacaag tcgctttccc ttatgatcca gaaattgcta
    16501 atttagttta tgacaaattg ctgaaagaag gattagccgt tcatttagaa acaagagtta
    16561 ctgagatccg tgataaaggt cgagaaatag tattatcaga tggctccgtc ctttctgctg
    16621 atatgctaat ttttgctgtt ggtgtttctc cgaagaatga agtggtgaaa gcaaccggca
    16681 tacaattatc agatacaggt cagatcattg ttgatgacca gttacaaacc aatctgccgg
    16741 acatctatgc gattggcgat attatcgaaa cgactagtgt agtgactggt cagccgatcc
    16801 aaagtatgct ttccagtgcg gccaatcgtc aaggacatat gctggcagat attttaaatg
    16861 gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt cgcaaaaatc tttgatcata
    16921 cagcaagtta tgcgggaatg acagaacatg aattaaaagc atcaggcata acaaattata
    16981 aaactgtttt tatcactccc tttgaccatg cctatttcta tccagaagct acaagattaa
    17041 atctaaagct gatttttgat gcagatagcg gtcgtatttt aggtggacaa gcatttggag
    17101 aaaaaggtgt cgataaacgg atgggagaac tttctgtagc aatcaccgga aacttgacag
    17161 tctttgattt gcctgatttg gagttgcctt actctccacc gtattctact acccgtgacc
    17221 cgttgaatat agctggttat gtcgcaatca atcaaatgac gaatatcgta gaaacgatca
    17281 aagcaagtga tatacccgaa aacgatttga aagaagcgtt ctttttagat atacgtgaac
    17341 ctaataaagc accttctggc agtatctcgg caacaaaaaa tattccaatg aatgagttac
    17401 gagatcgaat caatgaaatc ccaaaagata aaaaagttta cattactttc agaagaggat
    17461 tgaatactta tacttctgcc cgaatcttgg caggtttggg tatcaaagcg attttgattg
    17521 aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag gagttatttt tacttgagga
    17581 gtaaaaatga aaaagtatat agggttgttg gttggtatgt atatagaata gaagataaat
    17641 gtgaagaaat tgtgcattat ttcttcacat ttattaggtt atagtttaag aaagtttaaa
    17701 cattgcgcaa tttaattatt ttagctgttt cttttcataa aaaaacatgt tgtgactctg
    17761 agacaaatca tagtccatag tgatgtctct agagtaacta cgcctaatat tacgaggcat
    17821 catattaatc tatcagcact agtacctaaa ctagcatccc tttcgaatat tttttcttat
    17881 ataataaagt ttgctttcac tcctctaatc tcaccaaatc gactggcttg cgttgtgtga
    17941 tactaggaac tgattggttt agaacgactt ttctattgta atcatatttt tcgccatcct
    18001 cgttgtcttc taatccttca atgaatgatc cctaatgtgt gtattaaata atttaatttt
    18061 tttgttccta tttatttaac
//
LOCUS       DASJST010000053        18022 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJST010000053 DASJST010000000
VERSION     DASJST010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.053"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            214..498
                     /locus_tag="U4B05_001419"
     CDS             214..498
                     /locus_tag="U4B05_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN2050567.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="U4B05_001420"
     CDS             complement(815..1198)
                     /locus_tag="U4B05_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN2050568.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="U4B05_001421"
     CDS             complement(1200..1784)
                     /locus_tag="U4B05_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN2050569.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="U4B05_001422"
     CDS             complement(1831..3306)
                     /locus_tag="U4B05_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN2050570.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="U4B05_001423"
     CDS             3487..3798
                     /locus_tag="U4B05_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050571.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="U4B05_001424"
     CDS             complement(3913..6249)
                     /locus_tag="U4B05_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2050572.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="U4B05_001425"
     CDS             6391..7059
                     /locus_tag="U4B05_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2050573.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7043..8104
                     /locus_tag="U4B05_001426"
     CDS             7043..8104
                     /locus_tag="U4B05_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2050574.1"
                     /translation="MRRLFKDEKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRH
                     SISPAESAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFS
                     IRMDEQQKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLL
                     QAPNLSDEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREV
                     IVFFQPKWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFE
                     TATEIVLEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRI
                     DYSSIEGVGTTAMIRLSKQ"
     gene            complement(8341..9222)
                     /locus_tag="U4B05_001427"
     CDS             complement(8341..9222)
                     /locus_tag="U4B05_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2050575.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9381..10109
                     /locus_tag="U4B05_001428"
     CDS             9381..10109
                     /locus_tag="U4B05_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN2050576.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10265..10582
                     /locus_tag="U4B05_001429"
     CDS             10265..10582
                     /locus_tag="U4B05_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2050577.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10575..11183
                     /locus_tag="U4B05_001430"
     CDS             10575..11183
                     /locus_tag="U4B05_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN2050578.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11238..12029
                     /locus_tag="U4B05_001431"
     CDS             11238..12029
                     /locus_tag="U4B05_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN2050579.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12167..12655
                     /locus_tag="U4B05_001432"
     CDS             12167..12655
                     /locus_tag="U4B05_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN2050580.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12706..13578
                     /locus_tag="U4B05_001433"
     CDS             12706..13578
                     /locus_tag="U4B05_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2050581.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="U4B05_001434"
     CDS             13754..14116
                     /locus_tag="U4B05_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2050582.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="U4B05_001435"
     CDS             14125..14877
                     /locus_tag="U4B05_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050583.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="U4B05_001436"
     CDS             14891..16693
                     /locus_tag="U4B05_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050584.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="U4B05_001437"
     CDS             16970..18010
                     /locus_tag="U4B05_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2050585.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5657 a         3126 c         3723 g         5516 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa agctattttc
     7081 acaattatgg atctattttg ctagtattgt cttcatttcg atcttagtga cactgctgtg
     7141 ttttctcggt tttattttct tattgtctcg acattctatc tcgcctgctg aatcggctgg
     7201 aaaaccaacg ttatacccgt tattcgtctt tgcggggttc agtatggtcg tgggtacagg
     7261 aatatcgatt tttgttgggc ggcgtatcct gcatccaatc agtgcgctag ggacaaatat
     7321 gagtctggta gcaacgggag atttttcgat tcgaatggat gaacagcaga aagtagcaga
     7381 agtacagcaa ctgtataaag atttcaacgt tatggtccaa gaactcaata gtatcgagac
     7441 actgaggaat gactttgtgt ccagtgtatc ccatgaattc aaaacaccac tagcaacaat
     7501 ccaaggatat gttcaattgt tacaagcccc aaatctctct gacgaagaac gccaaatatt
     7561 tttatatcgg attatcgaaa gtatcacgca gctttcacaa ttaacagaga acatcttaaa
     7621 attgaacaaa ctggaaaacc agcgtatcca actggaaaag aaagagtatc gattagatga
     7681 acaaatcaga gaagtaatcg ttttttttca gcctaaatgg gagaaagaac agctagaatt
     7741 ggatatcgaa cttgctgcag taaattacac aggcaatgaa gagtttttgt accaagtgtg
     7801 gctgaatatc atggataacg ccataaagta caatcaaatc aatggacaga ttcatatcaa
     7861 actatttgag acagccacag agattgtact ggaagtgacc gattcaggtg taggaatgaa
     7921 tgaagagacc agagatcgaa tgtttgaaaa attttatcaa ggagatacaa gtcgtcaaat
     7981 ttctgggaat gggctaggac tttcactggt taaaaaaatt ctagaactgc atgatggacg
     8041 tattgattat agcagtatcg aaggagtagg aacgacagca atgatacggt taagtaaaca
     8101 ataggaaaaa gaataaaacc cctaaaaatt ctattgaagg tttttagggg tttttagtcg
     8161 tttttttgtg attagggaaa tgttcttacg gttcttttgg cataaaaatc gctgctttct
     8221 tattgaacta cgagaaatgt tagaggtgtc attgaaacaa ggacatgatg caagaaagag
     8281 agcgaaagtc atgcgtcgct ctcttttttt gggtggatag atggacagaa aatgtagggc
     8341 ttagttttct tcttgattta aatactccca aaatgtatct gaagccaaag aatgctcacc
     8401 gactttacga gtaaatgcaa gataactctc tgttgttgga gaaataggga gaatcacagt
     8461 attttctttt aatagattct tgactgtttt ttccataaca atagcaatcc ccatgtcatt
     8521 tgcaatcata ttgatgatca agtcgattct tgctccttta taagagattt ttggttcaaa
     8581 tccagcttcg cgacacagat ctagtacagg ttgatacaag ttcgtagtgg ggccaaggag
     8641 caaaaagttc tcctttttca gttcgcttag atccaatttc tttttactag ctaaaggatg
     8701 atttttaggt agaactgcta caaaatcgtc tgtttcagta ggcaaccact caatcgaact
     8761 tggtggggcc tggaaacttc tagcaaagtg gatcgtgtca ctatgttcat tggcgtcatt
     8821 gtaagttaat tcgacacttt ccacttcttt caactggact gtaaattcag gatggcgttt
     8881 caaaaactcg gtgattttgg aaaaaccttt ataattgatc attgtcggta tcgttaagat
     8941 atttaatgaa agattgttgt ctttcgtata ggtttcaagc acttcttgca tagcatggta
     9001 ttgttctacg acatttttta catatgggag caataagctt ccagcttcgg tcaattcgat
     9061 ttttctatgg gcgcgttcga ataatttgac gtctaattct gtttctaacg aaagaatctg
     9121 tttagaaata tttccttggg tggtgaattg tcgatcagca gttttggtga aattcattgt
     9181 ttcggctaaa tcaagaaaaa cttcgaattt atggatatcc atcatattcc tcctacttat
     9241 tccaataaag aatactttaa cacaaaaaat gaattggtgt aatcttctct gtctgttaca
     9301 atctttcttg cagcgaaagc aataattaca gaaatttttc tgattttttt cgcttcgtag
     9361 aaaaagagag gagttttttg atgccaactg gagttttgat caatgtaggt tccgttttgt
     9421 ttggcggact aattggcgga ttagtaggaa ataaattaag tgaacatttt aaagcacagc
     9481 tgacgatggt ttttggtgtg tgttcgatgg ggatgggaat ctattccatt gcgccgatga
     9541 aaaatatgcc ggctgttatt tttgctttag tgataggaac agcgattggt ttgattgttc
     9601 atttaggtaa tgggataaat aagggggctg cgctgatgca agtacctatt tctaaaatct
     9661 tccctagtga aaagcttggt atgacccacg atgaatttat cagcacatta gtgacggtca
     9721 ttgttctatt ttgtgcaagt ggtacgggga tctatggaag tttagattct gggatgacag
     9781 gagacagtac gattttgatt tctaaatctg tccttgattt tttcacagca gctatttttg
     9841 cgtgtaattt agggtatgtc gtttctgtga tagcaattcc ccaattcatt attttttata
     9901 ttttgttctt attggcaaaa tttatttatc cattgactac accagacatg atcttagatt
     9961 ttaaagcttg cggcggattc ttgatggtcg ctaccggttt tagaatgatc aacgtgaaaa
    10021 tgttcccagt agctgacatg atacctgcaa tgatcgttat catgccactg agctggatgt
    10081 ggacaaattg gattatgcca ctgttataat aaaaatcaaa gagaagccat tttttgctga
    10141 ctaaaaagga tcaaatcttt tagtcagttt ttttgtattt gacaaactga ttttgaaact
    10201 atattatatg ttgtataata tagattgaaa taaaatatta cacttgagaa tagaaggtga
    10261 cgtaatgcac atccagattc caactgttct tcttgatggt actgtgctag cagcattaag
    10321 caaggaagat atgtatgggt atgccttaac aaaaatcgta caagaaagtc tctctgtcag
    10381 tgaatctaca ctatatccgg ttttgagaag attgaaaaaa gaaggatatc ttgagaccta
    10441 tgatgaacct tttgaaggca gaaaccgtcg atattactcg ttgacaccaa tgggaagtcg
    10501 tcatttagaa gaaattattg atgaatggat tgtttttcga gattctttag accggttatt
    10561 ggaggagagc acaaatgaat gaatatttga atgaagttgt ggatcatctt caagaaattc
    10621 ctgaagacga tcgttttgat ttgatccagt attatgagga atatttttta gattctggaa
    10681 aaacattaga tcagatcatt gatgaatatg gtacaccaaa gcagtttgcc ttaaaattaa
    10741 aaatcagtta cttttcagaa gaagaacaag cagaaaacga gttagtgaaa aatccaaaaa
    10801 gacaaattcg cctgatttgg ttaatcatta tcggtttatt tgcttctcct gtcctcattc
    10861 cactagcact ggggtttata ttggttattg ggggactatt ggttgcttta ctagcgctca
    10921 tttttagtat ctatgtttta tgtatcagta tcttaggtgt tggcctattc atgatcatct
    10981 caggaatcag tgtgtttgga caatcgattc tgagtggatt attctttatc ggtgtcggtc
    11041 tgttcgccac aggaagcgca gtatttattt cgccactgtt gctgaaagga acaaaagggc
    11101 ttttttatgt catgatgaac tttgttaaat ggatgggacg acgttttatt acaaaaagag
    11161 atctgcattc agcgggcgtt tgaaggagga cacataaatt gagattgaaa cattttttga
    11221 ttaccggtat tggtctgatg cttactggtg gcctcctagc aggtgcaact tatacattag
    11281 gcgctcaaaa gagtctgact tgggataatg gtcctcggtt gatcgatatg gttactgaga
    11341 caaaaaaaat caaggatgca aacaaaatcg tcatccatgc agaaaatcaa acagtttcgg
    11401 tcatacgcgg attggatttt gagataaaaa cgacttatga taagacagaa aagccagaaa
    11461 taaaggagaa aaatggcgtg ttgactgtga atgcagtcaa aaaatcaaca ggtgcaatca
    11521 ttagtttaga agacacaagt gcacaaatca ctattgttgt ccctgcagat catgaattat
    11581 ctgaattgac aatcgaaggg aataatagtg caattgatat caacgaagta tcaatcaaaa
    11641 aagtgaatac taagacacat gatggctggg tatcgttcaa tgaatttaca ggaggaacaa
    11701 taactgcgaa agatgaatta ggctcacttc gattagatga ggccaatgtg gataacttga
    11761 tcgtcacagg tgaaaacagc aacttccatc tagaagacag tttgatcagc aaagaaggaa
    11821 aagtcatcct aaacggtggc aatttatctt tgcatgagat ggaaaacagt ggctatacta
    11881 tccaaacgtc tggtgaatct tatattgaaa aagactacca gccaataaca aatactttgt
    11941 tagaacaagg acagaaaaag ctaaagatca ctgctaaaga cacatcgatc aatattacga
    12001 cggatcaaga ggacaatgaa acagattgat cctgtactca actggatatt ttaagaaaaa
    12061 acagcaaaga tccttccaat ctttgctgtt tttcatcgct taacgcacat aactatagta
    12121 aaataagaaa gaaaatatct aaaaagaaaa cagggtgaaa gagtatatga aggtagaaga
    12181 aggtcaaaga gaaaagttaa aaacagaaat ggaaagatta catacttata tcactcaact
    12241 aagccaaacc ttctatgatc cagacaaaga aaaagtgatg gttaattacc cgaacaattc
    12301 agaaggtcgc caattagagc aagtgtacca tgaagttttc aaacatttat tgacggtgaa
    12361 gaaagagctg gattattatt ctttaccgat tatcgataca ggaatattga agtatgatgg
    12421 gaagaaggaa cgatttatct ttaaatctgt tcgggaagat ttgcctcttt cagcagggat
    12481 ggatctagag gtcttggtag aagactattt tacagaagaa aagcactggg ttcgaacaaa
    12541 attggattat ttaccgcagg cagccggagg gacacaggca agcggttggt acatcactga
    12601 agacaaagag ttagaactag aaggtgtaat ggcgcgtatt cgaaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcgt
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatattctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat ggaaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aa
//
LOCUS       DASJST010000054        17860 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJST010000054 DASJST010000000
VERSION     DASJST010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.054"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..636
                     /locus_tag="U4B05_001438"
     CDS             133..636
                     /locus_tag="U4B05_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN2050586.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="U4B05_001439"
     CDS             763..1800
                     /locus_tag="U4B05_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050587.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="U4B05_001440"
     CDS             1815..3512
                     /locus_tag="U4B05_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEN2050588.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="U4B05_001441"
     CDS             3505..3966
                     /locus_tag="U4B05_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEN2050589.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="U4B05_001442"
     CDS             3979..4839
                     /locus_tag="U4B05_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050590.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="U4B05_001443"
     CDS             4877..5266
                     /locus_tag="U4B05_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2050591.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5250..7700
                     /locus_tag="U4B05_001444"
     CDS             5250..7700
                     /locus_tag="U4B05_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2050592.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="U4B05_001445"
     CDS             7705..9813
                     /locus_tag="U4B05_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2050593.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="U4B05_001446"
     CDS             9810..10814
                     /locus_tag="U4B05_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN2050594.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="U4B05_001447"
     CDS             10833..11744
                     /locus_tag="U4B05_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2050595.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="U4B05_001448"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="U4B05_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="U4B05_001449"
     CDS             complement(12406..12621)
                     /locus_tag="U4B05_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050596.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13047)
                     /locus_tag="U4B05_001450"
     CDS             complement(12880..13047)
                     /locus_tag="U4B05_001450"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2050597.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13392..14831)
                     /locus_tag="U4B05_001451"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="U4B05_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="U4B05_001452"
     CDS             complement(15379..16050)
                     /locus_tag="U4B05_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN2050598.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="U4B05_001453"
     CDS             complement(16399..17850)
                     /locus_tag="U4B05_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN2050599.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         5819 a         3007 c         3463 g         5571 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc
//
LOCUS       DASJST010000055        17721 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJST010000055 DASJST010000000
VERSION     DASJST010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.055"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..858
                     /locus_tag="U4B05_001454"
     CDS             46..858
                     /locus_tag="U4B05_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN2050600.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            855..1655
                     /locus_tag="U4B05_001455"
     CDS             855..1655
                     /locus_tag="U4B05_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN2050601.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1816..3084
                     /gene="rseP"
                     /locus_tag="U4B05_001456"
     CDS             1816..3084
                     /gene="rseP"
                     /locus_tag="U4B05_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN2050602.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3152..4861
                     /locus_tag="U4B05_001457"
     CDS             3152..4861
                     /locus_tag="U4B05_001457"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN2050603.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5073..9431
                     /locus_tag="U4B05_001458"
     CDS             5073..9431
                     /locus_tag="U4B05_001458"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN2050604.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9576..10049
                     /gene="rimP"
                     /locus_tag="U4B05_001459"
     CDS             9576..10049
                     /gene="rimP"
                     /locus_tag="U4B05_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN2050605.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10072..11247
                     /gene="nusA"
                     /locus_tag="U4B05_001460"
     CDS             10072..11247
                     /gene="nusA"
                     /locus_tag="U4B05_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN2050606.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11269..11562
                     /locus_tag="U4B05_001461"
     CDS             11269..11562
                     /locus_tag="U4B05_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN2050607.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11559..11870
                     /locus_tag="U4B05_001462"
     CDS             11559..11870
                     /locus_tag="U4B05_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN2050608.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11883..14189
                     /gene="infB"
                     /locus_tag="U4B05_001463"
     CDS             11883..14189
                     /gene="infB"
                     /locus_tag="U4B05_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN2050609.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14216..14563
                     /gene="rbfA"
                     /locus_tag="U4B05_001464"
     CDS             14216..14563
                     /gene="rbfA"
                     /locus_tag="U4B05_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN2050610.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14720..14893)
                     /locus_tag="U4B05_001465"
     CDS             complement(14720..14893)
                     /locus_tag="U4B05_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2050611.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15341..17703
                     /locus_tag="U4B05_001466"
                     /pseudo
     CDS             15341..17703
                     /locus_tag="U4B05_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
BASE COUNT         6038 a         3153 c         3900 g         4630 t
ORIGIN      
        1 aatacttaag atagtgggaa tcttttaagt ataggaggaa aaagaatgtt acgttttttt
       61 ccgcaaaaaa acaaatatat tcaagaaaaa agcgaatttc aatttaatac cgatggacca
      121 attcctcagc acattgccat catcatggac ggcaatggtc gttgggcgca aaaccgtcgt
      181 ttgccacgag ttgctggcca taaagaaggc atggaaacag taaagaaagt cacaaaaaaa
      241 gcatcaaggc ttggagtgaa agtgttgacg ctttatgcct tttcgactga aaactggaag
      301 cgaccaaaag atgaagtaag ttttctcatg caactacctg tcgacttctt tgatacgttc
      361 gtaccagaat tgattaaaga aaatgtcaaa gttcatgtca tgggttatga aaacgtattg
      421 ccagaacata cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa caatactgga
      481 atggtattga attttgcttt gaattatgga agcagagcag agatcgtgac tgctgtccaa
      541 gaaattgccg aagaagtagc aaaaggagaa atacacgcag aagaaatcga tgatgaattg
      601 attgctaagc atttaatgac aggtttcttg cctaaagagt tacaagatcc tgagctgatg
      661 atccgtacaa gtggagaaga gcgaattagt aattttctgt tatggcagat cgcatatagt
      721 gagctgtatt ttacaaaagc gctttggcct gattttgatg gtgcgcatct tgaagaagca
      781 atcgcctctt atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac agaagatgag
      841 aaaggggatc agtcatgaaa caacgtgtgg tcacagcagc cgtggcgcta gtcttgttta
      901 ttccgattat atggatcgga ggcatagcgg ttgagcttac agcagcaatc ttagcagtaa
      961 taggagttta cgagctgttt cggatgaaag gattgacact cttaagtttt gaaggagttt
     1021 tatccgccgt tggtgcagtg ttacttgttt tgccaaaaga aagatggttt ttctttcttc
     1081 cagagaatgt aagtacattt atgttatttt atcttatagt tatgattctt ttaggaacat
     1141 cagttatttc taaaaatacg tatacgattg atgaagcagg ttttcctgtg cttgtcagct
     1201 tgtatgtggg aataggtttc cagaattttg tcaatgcacg aacagaaagc ttggctgttt
     1261 tgatatttgg tctatttatt gtttgggcaa ctgatatcgg agcatatatg gttggtcgac
     1321 gttatggtca gcataagtta agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc
     1381 tcggtggtat tggtagtgct gttgtagtcg catttcttta tttattgcta tacccagcaa
     1441 aagaactatt taatcatggt atgtttgtca tgcttctgat gacgattatt ttttcagcag
     1501 tcggacaatt tggtgatttg gtcgaatctg caatcaaacg tcattatgat gtgaaagact
     1561 cgggaaatat cttaccaggc catggtggaa tcttagaccg tttcgacagt ttgctgtttg
     1621 tatttcctat catgcattta tttggcatta tttagtaagt atacctgcgt ttctttgtga
     1681 atttcataaa agacgcaggt ttttattttt cattatattg atactattgg tgtctttggt
     1741 atactatggt aaaattatag tgttttatcg ttgttttata aaaaacacaa aaaacaagta
     1801 aaggagaaaa aagctatgaa aacgattctg acatttatca tcgtttttgg tatattagtg
     1861 attgttcatg agtttggtca tttcttcttt gcgaaacgat caggaatcct cgttcgcgaa
     1921 tttgctatcg gtatgggtcc gaaaatttat gggcatcaag caaaagacgg gacaacatat
     1981 acgcttcgcc tattacctat tggcggttat gttcgaatgg caggcaatgg ggatgatgaa
     2041 acagaaatgg caccaggaat gcctctttct cttttactaa attctgacgg catcgtagag
     2101 aagataaatt taagtaagaa aatccaattg acaaatgcta tcccgatgga attgagccgt
     2161 tatgatttag aagatgagct gactatcact ggttatgtga atggagacga aacagaagta
     2221 gtaacctacc ctgttgatca cgatgcgacg attattgaaa acgacggaac agaaatcaga
     2281 attgcgccaa aagacgtaca gttccaatcg gctaaattgt ggcagcgtat gctgaccaac
     2341 tttgctggac ccatgaataa ctttattttg gcaatcctgt tattcatcat tctggcattt
     2401 atgcaaggtg gtgttcaagt gacgaacact aatcgcgtag gagagatcat gcctaatgga
     2461 gctgcggcag aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga cggaaaagaa
     2521 atccattcat ggaatgatct gacaacagtc attacgaaaa atccggataa aacattagac
     2581 tttaagatcg aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa atctgtagag
     2641 tcaaatggcg aaaaagttgg ccaacttggt ataaaagccc caatgaatac aggttttatg
     2701 gataagatca ttggagggac gcgtcaagct ttcagcggtt cattggaaat tttcaaagcc
     2761 ttaggatcac tatttacagg tttcagttta gataaattag gcggacctgt catgatgtac
     2821 caattgtctt cagaagcagc aaatcaaggt gtaacaactg ttattagttt gatggcgctt
     2881 ctttcaatga atctcggaat cgtcaatctg cttccgattc ctgccttaga tggcgggaaa
     2941 ttagtcttga atatttttga agggattcgc gggaaaccat taagtcaaga aaaagaagga
     3001 atattgacat tagctggctt tggattttta atgttgttga tggtgctagt tacatggaac
     3061 gatattcaac gattcttttt ctagaaaaag ttgacaaata gggtataata acaaacgata
     3121 actattttga ggaaataaag gagtatatag aatgagacaa tcaaaaatgt tgattccaac
     3181 attacgcgaa gttccaaatg atgcagaagt attaagccat caaatcttgc taagagctgg
     3241 atatatccgc caagtatcag cgggtattta ttcatatctg ccattagcaa atcgtgtatt
     3301 ggaaaaactg aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg tagaaatgct
     3361 gatgcctgcc atcttgccag cagaactttg ggaagaatcc gggcgttacg aaacatatgg
     3421 accaaacttg tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg gaccaactca
     3481 cgaagaaact tttactgatt tgatgcgtaa tgaaatcaat tcatataaga aattaccttt
     3541 aaatctttat caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct tcggtttgct
     3601 tcgcggaaga gagtttatca tgaaagatgc gtattctttc catgcaacgg aagaaagttt
     3661 ggacgagaca tataaagatt acgaaaaagc ctacaccgaa gtattcaagc gttgtggctt
     3721 agaatttcgc tcgattatcg gagacggtgg cgcaatgggc gggaaagact caaaagaatt
     3781 catggcgatc tcagaaatcg gggaggatac gatctgttat tcgactgaaa gtgattatgc
     3841 tgcaaacttg gagatggcta caagctacta tgtatcaaaa aaatcgcatg aaacacagct
     3901 tgaattggag aaagtagcta cacctaacgt aaaatcaatt gacgaagtag cagatttctt
     3961 ttctgttgaa cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg aacaacctgt
     4021 ccttgttttg gtacgcggag accatgaagt caatgacgta aaactgaaaa actttttatc
     4081 tgctgacttt ttagaagaag caacagaaga agaagcacaa aaatatctag gtgctgagtt
     4141 tggttctgtc ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag accgttatgt
     4201 ccaagattta gcgaatgcca ttactggagc gaacgaaaca ggattccact ataaaaatgt
     4261 gaatccagaa cgcgatttca ctgtactttc ttatgaagat cttcgttttg tacaagaagg
     4321 agatccttca ccagacaaca atggtgttct tgcctttact cgaggaatcg aaatcggaca
     4381 tatcttcaaa atcggcactc gttacagtga atctatggga gcaactgtac tagacgaaaa
     4441 tggacgagaa aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc gtttattatc
     4501 agcaatcgta gaacaacatt cagatgaaca aggaatcaac tggccaaaag gaattgcgcc
     4561 atttgatttg catatcgtcc aaatgaatct aaaagacgaa taccaaacaa acctgacgaa
     4621 tgaactagaa gaagcgatga caaaagctgg ctatgaagta ctggttgatg acagaaatga
     4681 acgcgcagga gtgaaattcg cggattctga tttgattggc tgtccaatca gaatcactgt
     4741 cggtaaaaaa gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg gcgaaatggt
     4801 tgaagtacgt aaagaagaat tggtgaatac tttaccaatc ctcttgaacc aaggcgaata
     4861 ataagcgtta aaacacagaa aataaaaata gctgaatcag ctgtttttga caatctgatg
     4921 attcgctttt aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt aatgtaaaac
     4981 tcagaaaaaa cgagtatact aaaaggtact gaaaaagaag aggataagat gcgatcaatt
     5041 gcattgtttc tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat ttatttgaaa
     5101 aactgttgga acaaatccaa ctagaggaag cagaaaaaaa tcatccactg cttactgctg
     5161 gtgaaatgga tagtgtagtg gtccatcgta aatcgcgttt atgggaattt acgctgcatt
     5221 tttccaaaat tttgcctatt atgctatatc ggtcattgac tcagcatctg actatcgctt
     5281 ttaaagatat tgcacaagtc aaactgaata tcttagcaga tgaccagaca ttcgatgaac
     5341 agctcttgca agattactgg gcccaagctt tggaaaatca gcaatgcgat acaccactag
     5401 cacagcaagt attgaaaacg caggtacctg tgataaaaga tcgtaaagtc atcttgccaa
     5461 tcgacagtac aggagctatt tcttatttga agcaacaata cttgccatta gtagaagagt
     5521 tgttcgtcag ttacggattc ccaaaatttc gtatcgaacc agaagtggat gagcagcaag
     5581 cggaacgagt attgaaactt tttgaagaac gcaaacaaga acaggcagaa gcctttatga
     5641 agcaagcagc tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga aaagagcaat
     5701 cgccagctct tgaaggaccc atccacatcc agctaggacg aaatattcca gccgatgaac
     5761 caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt gaggggtacg
     5821 tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact ttgaaaatca
     5881 cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag
     5941 tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt caagaagata
     6001 cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa catacacctc
     6061 gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat agcaatatga
     6121 gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcaggg aaatggggac
     6181 accgagctat cgccattaca gaccacggtg gtgcgcaagc ctttccggaa gcacatagtg
     6241 ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc gtagatgatg
     6301 gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca tatgtcgtat
     6361 ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag ctagccgcag
     6421 tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat ccaggacatc
     6481 ctttgtcacg aacaacggta gatctgactg gtatcacgga tggaatggtt cgaggatcaa
     6541 agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca attttggtag
     6601 cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg tatggtattc
     6661 ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg tatcctcaat
     6721 tcaaacgttt cggattaggt gtgttatcta aaaaatttgg tgtcagcttg gaacagcatc
     6781 accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt gtgaaagagg
     6841 caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc ggagaaggag
     6901 attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat caggcaggac
     6961 tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac
     7021 gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg
     7081 ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca agaaaccgag
     7141 caaaattcta cgattatatt gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc
     7201 aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg gtagaaatcg
     7261 gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg aacgaagaag
     7321 atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc
     7381 atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca tttcactttt
     7441 taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc gcagatatct
     7501 gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca ggttcagaag
     7561 atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat ccgttacctg
     7621 agatcattca aaaacgatta aaaaaagagt tgaattctat taatggtaat ggcttctcgg
     7681 ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc tatctagtag
     7741 gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc actgaagtta
     7801 atccgttagc ccctcattat tattgtccag agtgccaata ttccgagttt tttgaagatg
     7861 gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct
     7921 taaacaaaga cggtcatgat attccatttg agacattttt aggattccat ggagacaaag
     7981 tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat aactatacga
     8041 aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca gtcgccgaca
     8101 aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag tttcgaagtg
     8161 cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc ggccagcatc
     8221 caggggggat catcgttatt cccgattata tggatgtcta tgattttacg ccgatccaat
     8281 atccagcaga cgatcaaaat tcggaatgga aaacgactca ttttgatttt cactcgattc
     8341 acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt attcggatgc
     8401 ttcaagattt atcagggatc gatccacaaa cgatccctac ggatgacccg gaagtgatgc
     8461 ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat tccaaaacag
     8521 ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgcta gaagaaacgc
     8581 atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt acagacgtat
     8641 ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg gaagtaatcg
     8701 gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc gggatggcat
     8761 ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat gaattgcgag
     8821 aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc gattcttgtt
     8881 caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg atggctcttc
     8941 gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac ttttctgtac
     9001 gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa
     9061 tgaaagaaat cacggacaaa ggattagatg cttccgtaaa agaaaagaat caactgacag
     9121 tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg attgatttgt
     9181 ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca ccatttagag
     9241 ctgtcccaag tttgggaacc aacgtggcaa aacaaatcgt tgaagcaaga aaagacggtc
     9301 catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat
     9361 atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta tcgttattcg
     9421 atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt atggtatagt
     9481 tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc tttttaatgg
     9541 aattacgcac ttaatttttt aaggaggcga aacttttgag tagcgtcgtt gaaacagtca
     9601 cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa gtcgaatttg
     9661 taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc ggtatcgata
     9721 tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca gcagatccag
     9781 atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag cggccattga
     9841 aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc ttataccaac
     9901 cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct gaacagctta
     9961 cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga aaaaatattg
    10021 caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa aatgagtaaa
    10081 gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg
    10141 atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg ccaagcacaa
    10201 aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa
    10261 gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc acttttaatc
    10321 aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa agattttggg
    10381 cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga agcagaaaga
    10441 acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa
    10501 cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa
    10561 caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt ttatgtttcc
    10621 cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca tccagaccta
    10681 ttgcgccgct tatttgagca agaagtacca gaagtctatg atggaatcgt tgaaattgtc
    10741 agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat
    10801 atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc aatcgtcaat
    10861 gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc tgtattcatt
    10921 gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac aaatccaaag
    10981 gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa acgcggacaa
    11041 aacgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc agaatctgac
    11101 atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa
    11161 gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt caacaatgaa
    11221 acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat gaaacaaagg
    11281 aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa agagatggtc
    11341 cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa aatgcctgga
    11401 cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga taaaaaaatt
    11461 ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact tcttgactat
    11521 gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt aatcggcaaa
    11581 aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac
    11641 tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca accgatgcaa
    11701 gcgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt ccttgctttg
    11761 agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag gtattcggaa
    11821 taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag gaaggtgatt
    11881 gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct agtaaagacg
    11941 tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt gcgattacta
    12001 gcaatgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg
    12061 cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa
    12121 caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc ggtcaaggga
    12181 gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg ggaaatacca
    12241 gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact aacaataaca
    12301 acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat cgttataata
    12361 aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca ccacgcaaat
    12421 tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca gatattgcga
    12481 aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg
    12541 tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg gactatggca
    12601 tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc ttcgaggcag
    12661 aagaagtcga tccagataaa ttagtctcac gtccgccagt cgtaacgatt atgggacacg
    12721 tagaccatgg gaaaacaact ttattggata cattacgcca ttcacgtgta acaagcggcg
    12781 aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat ggtaaaccaa
    12841 tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa
    12901 gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg ccacaaacag
    12961 ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct gtaaataaaa
    13021 ttgataaacc aggtgcaaac cctcaacatg ttatgcaaga attaagtgag tatgagttaa
    13081 ttcctgaagc atggggtgga gaaacgatct ttgttgagat ctcagcgaaa ttcggccaaa
    13141 acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat ttaaaagcag
    13201 atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc
    13261 ctgtgtcaac cttacttgtc caagaaggaa cacttcatgt tggtgaccca atcgttgtcg
    13321 gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc gataaagaag
    13381 caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc
    13441 gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa cgcgcgaaac
    13501 gtgcattatt agaaaaccgt gctgccacaa gccgtgtgac tttggataac ttatttgaaa
    13561 gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat gtacaaggtt
    13621 cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa
    13681 tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca gcagccagca
    13741 acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg caagctgata
    13801 ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa gaaatcgaaa
    13861 ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg
    13921 ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat gtaacagaag
    13981 gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta atctatgaag
    14041 gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa atgggttatg
    14101 aatgtggtgc aatgattgaa aaattcaatg acatcaaagt agatgacgta attgaaggat
    14161 ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa tcattatggc
    14221 taactaccgt gaccgccgag tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg
    14281 caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga aagttacggg
    14341 ggacttacag caagcaacga tctattacag cattttatct gacttagcat cagataagaa
    14401 aacagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt tagggcacaa
    14461 tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg
    14521 caaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa ctcccgcaaa
    14581 tcctgtgaaa tcaggggttg cgggattttt ttgttttcat taagacgtat tacctaagag
    14641 ttccatgact ttagtcttgc ggcgtgtcag ctaaccaaaa aagagaaaaa gaaaagtaaa
    14701 aaattcgact ttcttttttc tagtagtata taaatttttg catatagata aagatcaaga
    14761 taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat agccaagtaa
    14821 ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg ttgacagtta
    14881 aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga tttggtcacg
    14941 atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac caattcacat
    15001 attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag gaattgctcg
    15061 atccagcccc agttttccag caatgtgtta actggcccag cgtcattcag caaattatgc
    15121 atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag ttttttggat
    15181 caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct gttcatttgg
    15241 ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat tgggagtatc
    15301 tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt ctagtaaaga
    15361 atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg ttgggcaact
    15421 ttatcttaaa gataacccct tactgagacg tacactgaaa cccgaagatg tcaaaaaaca
    15481 tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc atttgaaccg
    15541 agtcatcaat aaatatgatt tgaacatgtt ttatattgaa gggccaggac acggcggaca
    15601 agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc cagaagtaac
    15661 ggaagatgag acaggaatgc aaaaattatt caaacgattt tcatttcctg gaggcattgc
    15721 ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc ttggatactc
    15781 tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg ctgtcgtgat
    15841 tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa atgtctttat
    15901 caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg caaaaatagc
    15961 caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact ttaacggact
    16021 tggctgggaa cctttcttta ttgaaggaaa tgattcagaa aaattaaatc ctgttatggc
    16081 tgaaaaaatg gatcaagcaa tcgagaaaat taaatccatc caaaaagaag ctcgcctgaa
    16141 gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca caccaaaagg
    16201 atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc gagcacacca
    16261 agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac tccgttggct
    16321 gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag aagaaatcag
    16381 agaaatcgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca atggtggaat
    16441 cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat ttgaaaaacc
    16501 aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg agatcattga
    16561 gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa accgattgaa
    16621 tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag aaaatgatga
    16681 atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc aagatgaagg
    16741 gttcttagaa gggtacgttc tgactggacg gcatgttttt ttgcaagtta cgaatcattc
    16801 ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatgcggaa atcacatgac
    16861 ctttcttggc gaaatgatta tccttcgcta aatctcattg caagctcaac agtcttccag
    16921 caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt ggcagaaaag
    16981 aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct cgcagtcatg
    17041 gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa acatccgcgt
    17101 gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt gaaaatcatt
    17161 gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc tgcaggaacg
    17221 gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt cccaaaacta
    17281 aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag tcaagatccg
    17341 cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc gatcttattt
    17401 gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca taaccatcat
    17461 ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga catgcgtgta
    17521 gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat caaaggaagt
    17581 caagcagaag attttgcgaa aaaaatggat cagaaattac aagaacatca aaactatatt
    17641 cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa tcttgaccaa
    17701 taaaatgata aaatcgatcc c
//
LOCUS       DASJST010000056        17713 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJST010000056 DASJST010000000
VERSION     DASJST010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.056"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="U4B05_001467"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="U4B05_001467"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2050612.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="U4B05_001468"
     CDS             complement(1197..2216)
                     /locus_tag="U4B05_001468"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2050613.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="U4B05_001469"
     CDS             complement(2849..3853)
                     /locus_tag="U4B05_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2050614.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="U4B05_001470"
     CDS             complement(3866..4915)
                     /locus_tag="U4B05_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2050615.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="U4B05_001471"
     CDS             complement(4932..5753)
                     /locus_tag="U4B05_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2050616.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="U4B05_001472"
     CDS             complement(5740..6519)
                     /locus_tag="U4B05_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2050617.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="U4B05_001473"
     CDS             complement(6536..7006)
                     /locus_tag="U4B05_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2050618.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="U4B05_001474"
     CDS             complement(7018..7437)
                     /locus_tag="U4B05_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN2050619.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10295)
                     /locus_tag="U4B05_001475"
     CDS             complement(7518..10295)
                     /locus_tag="U4B05_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2050620.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            10486..10710
                     /locus_tag="U4B05_001476"
     CDS             10486..10710
                     /locus_tag="U4B05_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050621.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="U4B05_001477"
     CDS             11489..13204
                     /locus_tag="U4B05_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050622.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..14970
                     /locus_tag="U4B05_001478"
     CDS             13207..14970
                     /locus_tag="U4B05_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050623.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(15010..15546)
                     /locus_tag="U4B05_001479"
     CDS             complement(15010..15546)
                     /locus_tag="U4B05_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2050624.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(15869..16291)
                     /gene="mgsA"
                     /locus_tag="U4B05_001480"
     CDS             complement(15869..16291)
                     /gene="mgsA"
                     /locus_tag="U4B05_001480"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN2050625.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            16500..17609
                     /gene="ugpC"
                     /locus_tag="U4B05_001481"
     CDS             16500..17609
                     /gene="ugpC"
                     /locus_tag="U4B05_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN2050626.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5222 a         3630 c         3099 g         5762 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtggc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg
    14941 ctctatcaag cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact
    15001 gcttgttttt tatttagcgt ggatcgctaa acttcttaaa atttttttgt aaggaatact
    15061 acgtcctacg taataaatgg caattgattg aatcggtaac catattactg tttttaacag
    15121 gcggggcgcc caaacgaccc agctgaacaa tggcacatgg tacatcaacg caagccataa
    15181 tggtgtcaaa aataagttga tgatcagcgt gacactaagc gtagccagaa tggcattttt
    15241 ccaagtgatc tcttttcgat agaaaaagaa tccgtagatt gctccttcaa taaatgcatt
    15301 caatgtaaat ccaataaaga atgcagattt cgagaagagg gccattccta ctaaatcagc
    15361 taaaactgcg cctatccctg accaaaaagg accaaaaaga atgcccatga tcacttgtgg
    15421 aataaatgtg acactgattt taataaatgg ggtctcgatt gctaccagtc gggacaaagt
    15481 gaccatcaat gcaatcaata agcccataat agcaatcata cgtgcgtcta accggtgttt
    15541 cttcattttt agcatctcct ttaagagctg ctacatcgta tcatgcagcg aatgcgaaaa
    15601 taaaaccgcg aacattacgt ccttcgtcca agaggcaaca tcccatcctc ttagcactta
    15661 acgctttatt ttctactctg tattattttt gggatctttt cccacagaaa ctatagcata
    15721 ttttcacatt tactgcaaat acgaaaaact aatattattg tttatattat ccaatacgtt
    15781 cgtattatat cgcatattat cagtttgttt agcttttata gctacatata ggcgaacatt
    15841 aaacgttatt tttccatacg cttatccatc aaaatgaaag tggatgcttg tctatttcat
    15901 ggacgattct tcgaaaatct aggaagccgg cttccagtcc tcttagtagc acctcagctg
    15961 ttccgatatt cgtagctaac ggaatctcat agacatcaga taaacggata agtgctgtta
    16021 catctggttc atggggctgt gcagccagcg ggtctcgaag aaaaatcacc aaatctaact
    16081 gatcctccga aatcatggct ccaatttgct gatccccgcc taacggtccg gatttaaacc
    16141 gatggatcgg caagccggtt gcttccgtga tcctctgccc agtcgtcccg gtagcaaaaa
    16201 gttcatgttt ttccaatatt gttttatacg ctgttgctaa tttcaccatc ataggttttt
    16261 ttcggtcatg agcgatcaaa gcgattttca taagagacac tccttttgat attgttaaca
    16321 tacttattta ctccagtgta tcagttttta tttcttatgg atatttatta aagcttaaaa
    16381 ctagcacatt gacaaaaaaa agaaaaaatt cttgttcaac atgacattga agttagttaa
    16441 cgctttcatt tatactttaa atagttaata aaactaaaaa agaaagtgtg gaacgtatta
    16501 tggtagaaat ggctctgaaa aacgtatata aaaaatatga caatgcagaa aattattctg
    16561 taactgattt taatctatca atcaaagacc gtgaatttat cgtttttgtt ggtccttccg
    16621 gttgtggaaa gtcaactact ttgcgaatga tcgcgggact ggaagatatc tctgaaggag
    16681 aactaaatat cggcggaaaa gtcatgaatg acgtggcacc taaagaccgt gatattgcca
    16741 tggttttcca aaactacgca ttgtatcctc atatgactgt ttttgataat atggcttttg
    16801 gcttgaaatt acgtaaatat gataaagcag acatcaaaaa acgtgtggac aatgcagcag
    16861 agatcttagg tctgacagaa tacttgaaac gaaaaccagc tgctttatct ggtggacaac
    16921 gccaacgtgt ggcattggga cgggcaatcg ttcgggatgc aaaagtcttc ttaatggatg
    16981 agccattatc aaatttagat gctaaattac gtgtagccat gcgtgctgag attgccaaac
    17041 ttcaccagcg tcttgaaaca acgaccatct atgttactca cgatcagaca gaagcaatga
    17101 ctatggctga ccgaatcgtt atcatgaaag acggatttat ccaacaaatc ggtacaccga
    17161 aagaagttta tgatacaccc gtcaatatgt tcgttgccgg ctttattgga tctcctgcca
    17221 tgaacttctt taatgtgaca ttgaaaaatg gtgttatcac agatggacat gggcttaagc
    17281 ttcgtattcc tgaaggaaaa aacaaagtat tagttgaaaa aggttatgaa ggaaaagaag
    17341 ttatcttcgg tattcgtcca gaagacatcc acagtgaaca agtagccctt gaagcttcac
    17401 ctgaagccgt agtgaaatca gaagttgtcg tttccgaatt acttggagct gaaacgatgc
    17461 tttacacaaa aactggttct actgagttca tctcaaaagt agatgcaaga gatttccata
    17521 aaccaggcga aatagtcgac cttgccttca acatcagcaa aggccacttc tttgataaag
    17581 aaacagaaca agtcatcaaa cttccataaa ttgccccctg ttagagctgt gccagaagat
    17641 gttggtgcag ctctttttag gacaaaaaac tctcatctta gaggatttat tatccattac
    17701 tagatgagag ttt
//
LOCUS       DASJST010000057        17663 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJST010000057 DASJST010000000
VERSION     DASJST010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.057"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            118..1083
                     /locus_tag="U4B05_001482"
     CDS             118..1083
                     /locus_tag="U4B05_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2050627.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1182..2354)
                     /locus_tag="U4B05_001483"
     CDS             complement(1182..2354)
                     /locus_tag="U4B05_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050628.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2341..3486)
                     /locus_tag="U4B05_001484"
     CDS             complement(2341..3486)
                     /locus_tag="U4B05_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050629.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3500..4432)
                     /locus_tag="U4B05_001485"
     CDS             complement(3500..4432)
                     /locus_tag="U4B05_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050630.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4549..5628
                     /locus_tag="U4B05_001486"
     CDS             4549..5628
                     /locus_tag="U4B05_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2050631.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5625..6243
                     /locus_tag="U4B05_001487"
                     /pseudo
     CDS             5625..6243
                     /locus_tag="U4B05_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(6353..6580)
                     /locus_tag="U4B05_001488"
     CDS             complement(6353..6580)
                     /locus_tag="U4B05_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2050632.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6683..9271)
                     /locus_tag="U4B05_001489"
     CDS             complement(6683..9271)
                     /locus_tag="U4B05_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN2050633.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9291..9950)
                     /locus_tag="U4B05_001490"
     CDS             complement(9291..9950)
                     /locus_tag="U4B05_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2050634.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9999..10502)
                     /gene="trmL"
                     /locus_tag="U4B05_001491"
     CDS             complement(9999..10502)
                     /gene="trmL"
                     /locus_tag="U4B05_001491"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN2050635.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10723..11589)
                     /locus_tag="U4B05_001492"
     CDS             complement(10723..11589)
                     /locus_tag="U4B05_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2050636.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11698..12339
                     /locus_tag="U4B05_001493"
     CDS             11698..12339
                     /locus_tag="U4B05_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN2050637.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12401..13762)
                     /gene="mgtE"
                     /locus_tag="U4B05_001494"
     CDS             complement(12401..13762)
                     /gene="mgtE"
                     /locus_tag="U4B05_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN2050638.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13780..14679)
                     /locus_tag="U4B05_001495"
     CDS             complement(13780..14679)
                     /locus_tag="U4B05_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2050639.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14681..15478)
                     /locus_tag="U4B05_001496"
     CDS             complement(14681..15478)
                     /locus_tag="U4B05_001496"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN2050640.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15456..16160)
                     /locus_tag="U4B05_001497"
     CDS             complement(15456..16160)
                     /locus_tag="U4B05_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN2050641.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16291..16866
                     /locus_tag="U4B05_001498"
     CDS             16291..16866
                     /locus_tag="U4B05_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEN2050642.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16984..17661
                     /locus_tag="U4B05_001499"
     CDS             16984..17661
                     /locus_tag="U4B05_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2050643.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5231 a         3792 c         3142 g         5498 t
ORIGIN      
        1 ttttttgaaa aaacaagttt ctttcattca aaaaatagaa aaagcttgct aattattggt
       61 taaccagcaa acaggagtat taaaatatct tgttttattg aatagcagga ggaaatcatg
      121 aaattccatt atcatatatt gatcaatcca tcggcaggca gcggaaatgg ctataaagta
      181 gcagaaagaa ttttaccggt attaaaaaat aagcatattg actatacact tcattatagt
      241 gaatacaaag gacacgatgc tgagattgct gaaactttgg caaaagaaac attgattcct
      301 tggatagaag aagaacagaa aacaatcgat actttccccc ttttgatcat cgtaggaggt
      361 gacggcacct tacatcaggt acttgatact ttctatcaga tggaagtaga attcccagtt
      421 gcttatattc cagcaggttc tggaaatgat tttgctcgag gggcaggcct tccaaaaaat
      481 cccaaaaaag gacttcagct gattttagca gctcaatcac cagaaaaagt ccacataatg
      541 gcttatgaag aaaaaataag tgaaaaaaaa ggaatagctg ttaataattt cggtatagga
      601 ttagatgcag ctatcgtcca tgcgacaaat cattcttcca caaaaaagcg attgaacaaa
      661 tacaacttag gatcattttc gtatttattc tctattctcc gtgctttatt cactcaaaaa
      721 ggatttccaa ttctggtaga tgcaggagga aaacaattaa gtttttcaaa cgcattttta
      781 tgtacagcaa cgaagcatcc tttttttggc ggaggaatcg cgattgttcc aactgctgat
      841 gcgtctaaac cagtcattga ttttgtcgtg gtcgaacgaa tcaatttctt taaaatcttg
      901 tggctcattc ttttacttat acagaaaaaa caattgaaat cgaaatattt ccatcactac
      961 acaacaaaca gattacgtat cgtttcaaca acaccgcaac atggtcaaga agatggagaa
     1021 gaaatggaaa aacgaccttt tgatatcaca ataaccacaa ctaaacagtt gttttggtgt
     1081 taataaaaga ggttttgtca gaagctacat ctggaacacc gtttattcgg aaaaaaggac
     1141 tgtgacacga cttttgtcac agtccttttt ttactatgca gttaattctt tgcccaattg
     1201 ggttgccatc gatagtttct ttctttaccc aaaataaatt ggatcattga aaacaccgca
     1261 gcaaaagcaa ctaagacaca accattgaac caaaaagatt ctgcaaatgt ttgattgtaa
     1321 ctgagtgttt cactgatcaa gttattattt tgaacaaacc aatatgttgg tgtgaatgcc
     1381 gctattttgc taaccatttc tggcaagtat tctgctggta caaacacccc accgataaag
     1441 caactgcctg tgataaaaat atctttgatc ccaccgctgg tttctgagtt ttttaccaat
     1501 gaagtgatac aagccgaaaa gctaatgatc ggcaataaaa acaagaaagt atttaaaata
     1561 tgataccata ccgttatatc ccacgaggtg tggatcacta acagaaccat tagaagtaag
     1621 aagccactga acaacagcag cgagtaacta atcaaactaa aggttacttt gcgattcaag
     1681 tttcgtttgg taatgggcgc actctggttt cgtttactga tctctttgcg attaaacgct
     1741 agattgatga cgctgaaacc gctaaaaatg gatagaaaca acccataaga caagaaatta
     1801 aaggttgtgc ccgtcaacag ctttttcatc cgctgactat atcccgcact tagctcaact
     1861 tctccagtaa ccgagagtgc catttgtgtc tgttccatag ctgcatcgat tgttttatct
     1921 tcttcttgaa acagaatcag tgtttgcaaa aaggtgttca cttgctgtgt aacaactgtt
     1981 ttagaaaagg catctgggcg actctgtatc ttgatcgtcg ccatctttcc tacttcgact
     2041 tgttcggtaa agtctgcagg cagatgtaag ataatgttta cacgattaaa ataaagagca
     2101 tcatcgattt ctttttgtga ggttccatcc aatgtaacga ctgtttcctt gtcattcaaa
     2161 tatttacgaa ggccatcgac tacaggcgac tcttcttcac taatcaaggc gattttcgca
     2221 tcttgcagag aggtattttc aacatcaata ttttgcgcaa aaaagaatgt ggcaacaaca
     2281 gtgataatta cacctatcag taaattcgat ttatttgctt taagcaatgt ccaaatcgtt
     2341 ttataaagcg tcataacgca cccctctttc catccaagca tttcctaacg caaaaaggaa
     2401 aataaatccg atcaaccata gaagattggt ataaaaacta gccaaatcag tgtagaaata
     2461 aagtttatac aaactttcgc tgatcaagtt gacgagattt aattgaccaa caatcggaat
     2521 atgaagatca atccaatact tcacttgttc cgaccccatc ataccagcaa aaaagctcat
     2581 cgcaatcgtc actgaaatca agatgctatc cttttgcacc agattcattt ttggtatcaa
     2641 attaccaacc aatgtaccta acaaaatcgc acacaacgac cccagagcgg taacaatcat
     2701 caaccattgc caacgttcac caaagtctac ttgatagata aaacgaaaga cagctaacat
     2761 aatataaatc tctgtcaaaa caatcgtaaa gcttgctgcc aaattagcga ttgaaactaa
     2821 cgattgcttt ctaggaatca aggacaagcg aataccattt gacgattgat ttgcttgctg
     2881 atcctgtaca ttgcggaccc cccacatcgt cccgtagaga atcgccattc caatcagagt
     2941 gaagaaataa aaacttttga aactcatatt acgacctgca tgaacttctt gaaaagatac
     3001 ttcttgttca aaaacagtca tgatgggcgc gagatcaacg ttctccactt ctgccacttt
     3061 actcgaaatt ataccaatac gctgtaaaaa ttgatccatg acgtttttca tcaacgtctg
     3121 actcattcct gcttgaccca catggagcga aacagatgaa ccatcaatga gataaaaacc
     3181 agccaaatca tctgcattta gcttttcatt cgcttctact tccgatagtt cctgtacttc
     3241 aaataatggt ttatcattga cttcgacttg ttctaagact gtggtaaaat tttccgaaag
     3301 cgctgtttga tccgctttga ttacaattcc agtattaatc gtttcaagat tgccaccttg
     3361 atcaatctcg ccaaaggccg ccataaacat caatcctaaa agaattggaa aggccaatgt
     3421 ccaaaacaac atggatttat ttcgcagcag tatttttgtg cgatatttat aaaaatgcca
     3481 aaacattatt tcccctcctc tagtctctca aatccttgcc agtaatctct aaaaagacat
     3541 cattcaagga cgcttctttg gttgctattt ttccgtaact gatcttttct tcttgtaaga
     3601 ttgtaagaat cggcaacaat actgtattta gttcagtagt tgttacttgg agctgacctt
     3661 ttttataagc gattccatca gaagctattg ttcgctgaat cgctgacaac acattttctg
     3721 ggagtaatgg gatgtcgatc gtcaaacgat ccgccgtccg taccatatct tttagagatt
     3781 ctttggttcc tttagcaatc acctgtcctt gatccatgat cacgatctgg tcgcaaagaa
     3841 tttctacttc ttccatgtaa tgtgttgtat agacaatggt tgctccttgt cgattcagct
     3901 ccttgatact ttctaaaatt ttattccgac tttgggggtc tacagctact gtcggctcat
     3961 ctaaaaaaat taaccgcggc ttgtgggcaa tcccacatgc gatgttcaac cgacgtttca
     4021 agccaccact aagttctcct ggacgaaacg aaacatattt ttctaatccc actaattgaa
     4081 tcacctcttc aatcaaggct ttccttttct ctttttcgga aacatataaa ccgcaaaaat
     4141 aatcgatatt ttcttgcaca cttagttcgt caaagaccgc aacgtcttga ggaacgaccc
     4201 caatcattgc cttgacagca taattttcag gagtcattgt tgtcccgaac aatttaatgg
     4261 ttcctttgtc ataatttaac aaagaaagca tacagttgat ggcagtcgat ttcccactgc
     4321 cgtttggacc taacagacct aagacttctc cttcttgtac ttgcagatca aaatgattca
     4381 atgctacaaa atctccatag cgtttgacta atttatcaat ttcgataatc atcatgaaaa
     4441 actccttcca aatttcttgt tgatctgact ataataaagt gcaagaaaag aaactagtga
     4501 cgattggcta aaccacacat gacatttgtc acaatgaagg agtctgctat gcataaatct
     4561 cggctactca acgaaactag ctttctactg gttatctcat tgcttttgct ggtaagtcaa
     4621 ggattcgttg ctatgcaagt tatctattta ttgatcgccg ttttattcac tggtgtcagt
     4681 tatttactgc ctacaaagcc aaattggatt ttccaagtta ttctatttct tttatgtgcc
     4741 tttttacctg atcttgtcct gtttctgcca gcgacgatgc gagtgatcgc ccctgaagaa
     4801 agccaccttg attacttgtt tttgttaggt tttttgttga tttcgacggt tggtaatcat
     4861 ccccccatca aggtcgcctt tgtcgtaatt ttgctgtttt tttcctttta cttacgaaaa
     4921 aaagatcaac gaacgaccga tcttgagaaa aagtacattg tctccaaaga tactcattgg
     4981 gaaaaacaac aagagttatc ggaacaaaat aaagcattgg ccctgtcaca agaaacacaa
     5041 ttagccttgc aaattgtgga agaacggaac cgaattgccc gagacatcca tgacaatgtc
     5101 ggtcatctgc tgtcaagtgc gattttacaa attggtgcgc ttgaaatgat caatcaagaa
     5161 ccaaaactaa aagaaccatt agaaaattta agtgccaccg ttcacactgg gatggatcgg
     5221 attcgtcaaa gtgtccatga cctccatgag acatcgatct cttttcaaca ctccttagag
     5281 tttctgatcg ctgattttcc ttcttctgtt acgattgaag gaaacttgtt tgaaggattg
     5341 aatgaaacgc agcaaaactg cttcttaatg gtcatcaaag aagccttaac caacagtatc
     5401 aaacatagtc atgccaaaac cgttgtttta cattttcgaa cgttgccggc cttttatcgc
     5461 ttgcaaatca cgaatgatgg caccagccca caaaaaaatt ctgctcccgg tattggtttg
     5521 aaaacgatgc gtcaacgtgt tcaagaacaa aagggacaag tccatatttt ccaagaaaac
     5581 cagcaatttc agttaacgat cattttacca agggagggac accaatgata acagtagtca
     5641 ttattgatga tgatccattt gtgacgaaat cgttacaaac cattttagaa agcacgaaag
     5701 agatccgtgt gttaggcatt ggtcattgtg ccaaggatgc gcgaaaccca tcgtcccgat
     5761 gttttgctga cggacatccg catgcctaaa caaacgggca tcgatgctgc caaagaaatt
     5821 ttggctaaat ttcccaaagc catccttttg cttttgacga cattcaaaga tgaagaatac
     5881 atccgcgaag cattttccat cggtgtcaaa gggtatttaa tcaaacagaa cttgcaagcg
     5941 attattcctt ctgtcaaatc cgcttataat ggacaggccg tatttggtaa tgaaatcatc
     6001 gagactttca ctcaaatgat gaagaataaa ccgacgattg atagtcatgc ttatgcttct
     6061 tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg ccgggaaaaa caataaagaa
     6121 attgcagatg ccttgtactt gagtgacggc actgttcgca attacatcag ccaattacta
     6181 gaaaagctag atttacggga tcgcacgcaa ttggctatct atttttatca gcatttgcaa
     6241 taagtcttat ctaacacaaa aaaacctgaa tgatgctcct tcgcatcatt caggtttttt
     6301 tcacatcttc tattcaattg tcccgcaaac catttcctca cgattgaaag aatcaaggat
     6361 tcgcagggct gccaggattc gttggattgt tcggcgtgtt aggcggaaaa ataggcgcct
     6421 tgtctaacgt tttttctagt tttgattctt cacgatcgtc tcgttctaca tcaggacttt
     6481 cattcaattt gaattctttt tccatctcat ctaacttttt aaagtcattg ccagattctt
     6541 ttgctaattt cgctaattta tcttcggttt ctttagccat aaagtaacgc cccccttttg
     6601 atttcagctt agcatatcac actgaaaaaa caaaagaaaa cgctaaccaa cgatcatttt
     6661 tagctatttc tcatctttaa aatcaagcat tttttgccgg tataaaatcg tagggagaac
     6721 tgttttccat accaatcatc ggatctacta actggttatc gatcacttct gctgtaagaa
     6781 cgaaatcttt agaaacagca ggttctacct cttctttctt ttcttctcca acaaatgaag
     6841 aaaacagatc tggttccact gtttccatta cttggttttt cgtttcattc gtcccactgt
     6901 catcttctac aggtagcttt tcttttacaa cccgtggctc ttttgtatag ttcccatctt
     6961 caaatggatc atttccgacc aaagaatctt cataagctgc tagtttggtc cggagagtga
     7021 tattcatttt atcattatcc atgatccttg atttcaacgt agtcatccgg cttgctgatt
     7081 cattcgctac acaagtgaca taggcttgct cttcccctaa caaaatcaac agttccttac
     7141 tgcgagtcac tgccgtgtag agcaaattac gctgaagcat ccgctgatat tgatggacca
     7201 tgggcaaaat aaccatcttg aattcactgc cttgtgattt atggattgag caacaataag
     7261 aaagagtgat tttattccat tcgtttcttt tataagtgac ctcatttgca tcaaactgga
     7321 taactaattc atccacttta tcttcagaat cttttgccaa gatgataccc gtgatttctc
     7381 ccatatctcc attgaataca ttcaattcgg gagtattgac taattggagg actttgtctc
     7441 ctatccgata aacggtgtcg ttccaatgta cttccttttt tccaggtttc cctggattga
     7501 atatctcctg catcatttta tttaacgcat cgatccctgc aggtccccga tacattggtg
     7561 ctaatacttg gatatcttgt gcacggaatc ccttattttt cgctttttcc acaattttag
     7621 agatcaaggg ttcaatttga taagcatcac ttcggaaata ggaacgatct ttttgattct
     7681 ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg ggcaagagga ataatactag
     7741 aaccatcacc ttgccgataa atctgattga gttccatttt gggaatttgt tcgatttcca
     7801 aaagatcatg gagtacttga ccaggaccga cagagggaag ctgatctttg tctcctacta
     7861 agataacttg catattcgtc ggaatcgctt tgaataatgt gttagccagc catgtatcga
     7921 ccatagacat ctcatcaaca atcaaaagtc cgccttctaa ttctttcgta ctgactgaag
     7981 gtgttttttc ccgtccattc agccccagaa gacgatggat cgtactggca ggcagacctg
     8041 tcgtttcgtt cattcgttta gctgctcggc cagttggtgc tgcaagtaga ataggaaaaa
     8101 tttcttgggt atattttgaa ggatccaatt ccagtccgtt caactctgca aaaagattga
     8161 cgattccgtt gatcacagta gttttccccg tacctggtcc tcctgttaaa ataaatagag
     8221 gggaacggat tgcttcagcg atagcttgtt cttgcgaagg tccatactgt atgccgaata
     8281 ttttttcgat acgccgaata ttcttctgca cttctttttc aggataatgg atttcttttt
     8341 tacgactgag aaggcgctgg atcgaattac cgatccccca ttcagaaaag tacaaactgt
     8401 tttcgtaaag attagtctct tcttgttgta tctttccttc ttcaacaagt tggataatta
     8461 cagtagcaac ctgatcaggc tggatttcaa ttgggcgact tgtttctaaa atacggatcg
     8521 tttgctgaag caatgtctcc gccggtacgt atgtatctcc tgtttgaact gcatgctgaa
     8581 aaatctgatg caagatagct gctcgaattc ttctcggtga atctgctgca ataccgattt
     8641 gttcggctag attatctgca cgtttgaacc cgattccttc tatgtcttcg actagctggt
     8701 acggattttc ttcgatgata tccaaagact cgttcttata ggcctggtag atactgaaag
     8761 ctaactgact accgaacccg taacgattca gtcccatgat cacttgatcc atcccgtggt
     8821 ttagccgaat cgtctcagca agcatttctc tttttggctt cgtcaaaccg gagatttgtt
     8881 ttaataccga aggatcagca atgatttttt cgatagcatc ttctcccagc aattctacaa
     8941 ttttttctgc tgttttcttc ccgattcctg ggaatttttc gctggaaaga tacgctacga
     9001 tcccatttgc agttgttggc ttttcttgct catatctttc gagtttaagc tgccttccgt
     9061 atttaggatg atccactaat tctccataaa aacgataagt ctcattttct tgaatatcgc
     9121 cgaaacttcc ggtgactaca atctcttttt cacggtaatc agctgtggtt tcactgatgt
     9181 gtaccaaaag cactttgtaa aaattactag gattttgaaa aaaaatcgcg gcaactgtac
     9241 caacgaaata aggcatttct tctggctgca tcggaagccg cctccttctt ctatagtaaa
     9301 ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt tccatacttt cagttcatat
     9361 ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca aactactatt gaacaatcca
     9421 cccatttcac gcaactcact tagctctttt cctgtcatcc attgaatggc tgcagttaaa
     9481 gaagctccat gtcctacgaa taaaagtggg ccatctttat gtcgggcaac cgcatctgct
     9541 acaacagttt cgatacgtgt gattgccgat tcaatcgttt ctccgtgaaa aggagaagga
     9601 tcatacagat ctaaatgatt acgcaaattc ggcagattat ccggatattt ctggtacatt
     9661 tcatcgatcg actgaccttc taaagtaccc agccctagtt ctcttagctt atctgtataa
     9721 aaaatctcta ccggacgagc aagctctttg ttgatacctt ctgctgtatc acgtgctcgc
     9781 tttgctgtac tggaataaat tgctttaaaa ggaacatatt ttaccttttt cccaaatgct
     9841 ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc cattgctccc ttgaaaacgt
     9901 ctttcctgat tccattgtgt cttcccatgt cgggtaaaat atagttccat acgcagcaaa
     9961 ctcctgtctt tcttttattt tactctataa taaactgtct aatccagctt gtcattttca
    10021 tagtgatgcg tcaattctaa attagggaat ccttgctggc gaagtgcttc atacactatc
    10081 attgcagctg tattcgataa attcaatgag cggacatgct catcattcat agggatgcgt
    10141 aaacactttt cttcgttttc acgcataaat gtttctggca aaccggttgt ttcttttcca
    10201 aataaaaaga agtgctcttc tccatcagca aaattttcgt cactatatac tttatgggca
    10261 aattttgaga tcaaatgtag tttacgatta cctaaaaatg ttaaaaaagc ttctaaatta
    10321 ctgtggtaag taatatttac atcattccaa taatccaacc ctgcccgttt tagatgtttg
    10381 tcatcagttg aaaaacctag tggctcgatt aaatgaagcg gagtattcgt ggctgcacac
    10441 gtacgggcaa tatttccagt gtttgcaggg attaatggtt caaataaaac gatatgattc
    10501 atcaggatca ttcctctcat atttatgttc gtttctattt tctgacaatt tgatttttgc
    10561 ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag tcgttacgtt agtgaagctc
    10621 ttctcactaa cttttatcag aacaggaacc tgataaaaac caatgaaaaa acaacttgtt
    10681 gctaatataa caccctcaac ttttttttgc aacctctact cattaaaaac tgctagaaaa
    10741 acgttttctc ttagttcctt ttaataattc tatgtcaatt gtaatcttct tcaaaggatt
    10801 cttatataag tctgctttga ttcgctcttc tacctgccat tctaaaggtg acatggccaa
    10861 aagatctttt tgattttcct ctggtatctc aaattcatag gttacacgct gacgttcagt
    10921 taatttcatt gcttcagcaa acttggctac tacacgttca ttggaataac tctgtttttc
    10981 aggttgattc ggataaaaag cctgacgtag ttcctttaaa taattttctt gtggcaccac
    11041 cttcaacact tctccatctt ctgagagtac acgttggaat tcttggtaat gagaaggcga
    11101 aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac ggcaaattgg tcaaatcagc
    11161 tacacaccaa aatgcttcgg cagattgttc tgtagctgcg tagactcctt cttttgaaat
    11221 atcaaatcca acagcatttt gaatcttttt caattttgaa agttcattca agaagctacc
    11281 ttctccgcag ccaacatcta atagcgagac ttcttcaggt aaatgctgcg caatagtctc
    11341 aataacgggc tgatacatgc cagattggat cattctacgc cttggaacga acattccttg
    11401 atcgtattct gttttaaccg catgggataa aaaataaagt gttccttttt tggaaaggtc
    11461 aaaacggtga ttatttttgc atatcaagcc attatcagac ttttttactt cttcttggca
    11521 aaccggacag cggaacagct gatgattttc tgccaaaaac tggcgtcctc ggtcaatttt
    11581 ttttaacata gttccccctc atttctgttt tatcttaccg ttagatgctc actttggcaa
    11641 ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag ataaaggaga agattagatg
    11701 atcaaagaat tttgcgctga aaactacaca tctattcctc tagcaattgc tagaggagcc
    11761 gatcggatcg aattgtgtga caacttagcg gtcggtggca caaccccgag tactggtgtg
    11821 atcgaagaaa cgttaagtta tgctggggaa aaagatatcc ccgtaatgac gattattcgt
    11881 cctagagggg gcgacttcgt ctataacgat atcgaattga aaatcatgca tactgatttg
    11941 attgaagcaa agaaattagg gacggatggc gtagtatttg gttgtcttac tccttcaggc
    12001 tggctagacg aagaggcctt agaactattg atcgaaacag cagaagggtt gcagatcacc
    12061 ttccatatgg cttttgacag tattcctgaa gatcggcaat tcgaagcaat cgattggctg
    12121 gtagaacatg gtgttgaccg tatcttgact catggcggtc ctgctgacaa aactattgta
    12181 gaaaatttcc cacggcttaa agaattgatt gcctatacag atggccgctt aatcattttg
    12241 cctggtggtg gtgtcactgc agagaatgca gcacatgtag ctaaagaatt gaacgtaagc
    12301 gaagtacacg gaaccaaaat cgtggatctt cagttataat cttataaaca aaaaaacatt
    12361 tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt ttacataaaa tagcccataa
    12421 ataaagaagc aatattgaaa taaatcagta cactcgtcaa atcgctcaaa gtcgtgataa
    12481 agggaccgct tgctactgct ggatcaaatc ctagacgatc cattagcatc gggattaaac
    12541 tacctgcaag attcgccact gtgattgcaa atagcatagc catccctatg acaaacccca
    12601 agataaaatt atgtttccaa aaacctacga ttagaaaaat cgtgatcccc gtaacagctc
    12661 ctgtgacaac gccggtcaag acttcagcca aaacaagtcg aacaaatcca tgatctttct
    12721 catcagaaat cgcaagtcga cgaacagcca cagctagcga ctgggttcca gcattaccgg
    12781 cagttcctgt aatcagcgaa atgaagactg ctaaaatact cgcttcactt accaagtctt
    12841 catagtgact gattaaagaa gcggtcgaca tacctaaaaa caacagagtg atcagccaag
    12901 gcaaacgtcg agacgctgcc tttactggat tttcattaat ttcttccaca ttgaccccgg
    12961 caagtcctga ataatcgcta gcagcttcat catcaataac gtcgatgata tcatcgactg
    13021 tgacgattcc taacaaatga tcatcataat cggtaacagg aagagctaag aagtcataat
    13081 ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac atgaactgaa agcactcgtt
    13141 cgctcatcag atcagaaatc attgcgtcgt catcattcac gattaggtcg cgcagagaaa
    13201 taacccctac tagctgacct tcttgattaa cgacatagat gtaatagatt gtttcagcta
    13261 catctgcttc gttcttcagc acgtacatcg cagagcgaac agtttgattg gctacgatcg
    13321 aaacaaattc agttgtcatg atcgcgccag ccgtttcatc ctcgtaatgg agaagttcct
    13381 tgatttcact ggcatcgtct gccgacatca aacttaaata tttagcaatt tgctttttat
    13441 ccagcgtatt aagcaagtca acagcattat ctgtatacat ctcagaaagc atatcagctg
    13501 catagctagg acgcatttca tctaggtatt ctttcatatg ttcgttgtct tcttcaatca
    13561 catcgaacat atccgctaat tctttaggcg acagatagga atagatcagc tgcctgttct
    13621 cttctgtaag agattggtag aattgcccct gctcatagat atgcatctct aaaaaactgt
    13681 ttctgaatgc ctgaatatta ttttccctta aatcttgagt caactgggca aatctttcct
    13741 ctaattcttg attttcatcc aaaccaatct cacctcgcct tattcttcaa accgtctcac
    13801 aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc agcaactctt ctgtaaatgg
    13861 atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt tttattccca gatccattcg
    13921 cccgccatac atatcatcac cgagcagggc acagccaatt gcggaaaaat gaactcttat
    13981 ttgatgtgtc cttcctgtat gcagctggat atcaactaac gcgatttctg ttagtcgctt
    14041 ctctatccag tactcagtct gtgccgaacg tccttcatca gagaccattc gcttcaatag
    14101 cgaagaataa tctcgggcga tcggcaaatc gatatgtcca tgtgccgata aatctgaaag
    14161 ccttccactg actaaggcct gatatttttt cacaaacttc ttttcacgaa gttgcatatc
    14221 taattttgcg tgcgcaaatc catgctttgc aaaaagcatc aagccagatg tatcccgatc
    14281 tagtcgcgtc accacatgga ttacttggtt ttcgtaacct tgttttttat aataagcctt
    14341 tacacgatta gccattgtcc catttggatg gtattgggca ggaatagacg aaacgcctgc
    14401 tggtttattt actaccagaa cgtgttcatc ttcatacacg atatcgatcg gtgtttcatc
    14461 caaaagtacg gtttcatgtt ctccttcagc aggaatcaca atggttacct tatcatcctt
    14521 agctagagaa aacaagacat tttctacttg atcattaact ttgatttgac cgccttgaaa
    14581 ttttattttc gctaacagac cctttgagat ccctttttct tttaaaaaat attttacttg
    14641 ctgagaatga tctttctcat aatcccatgt aaactgcata tcaatcctca ctaataaagg
    14701 catctttcac acgatgccaa aaatgcatat gtcgataaga agcaaaatgg atccgttctt
    14761 ctgctattcg ataataaatt gagcggatat ttgcttttgc gacattcaat tgatcaaccg
    14821 ttacaagata atcttctgta ttttccaatt ttatttctag ccattcatca tgggcaatca
    14881 cgatcggaga acccaaagta cgaaacactc gattattcag tgaagcaatc tctgccaact
    14941 gaaaggcatt gatactagga tggatcacag caccgccgac acttttatta tatgctgtcg
    15001 aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa actttcaaac agctcgtttt
    15061 tgatgtatac atcggctacc atcgtacgat tggcccgttt aatcgttgat tcattcagtg
    15121 ctaaaaaatg ctggtccggt gtttcatcca aataactgat ccgaacatcc agcaagggat
    15181 aactaacact ttgttcccgg ttcgttcgta agctgtctac tagttcttcc agctcgtaat
    15241 cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac gcccaagaaa cgcacatcat
    15301 tcagacaatg actgtagtga tggaacgccg acaataatgt gccgtctccg ccgactgaga
    15361 tgaccaattc tggctcatga tcatctcgtt gaatccctgc ttgttctaaa agtgcgttca
    15421 aacgttctgt cacttctata gtctttgctt ctttattatg aacgatcgct actttcatcc
    15481 cctgcctcct ctctttcgct ttctttcttc aaaagcgttc gatagtagac atcatctgat
    15541 tttcctcttc catgggagaa taaccgctgt gcttcttgga tttcttcacg gatactggac
    15601 atctcttcat ccaactgata ggcagcttct gccgcacgct gaagtcgttc actcatttct
    15661 tctggaaaaa cgccttgata tttataattc agcgaatgct caatcgttgc ccagaaattc
    15721 atcgcaagag tccgaatctg gatttccgcc aaaattttct tctcaccgct gatcagctgt
    15781 acaggatact cgatcaccag atgataagaa cggtagccac ttgctttttt atttgtgatg
    15841 taatctcgtt cttggagaac tttcatgtct tttcttttac ggatgatctc aacaacttga
    15901 tggatatctt ctacaaactg acacatgatc cttaacccgg caatatcaga catttcttct
    15961 tctaaacgtc ccattggaat atgccgtaat tgtgattttg ttacaatact atcgactggc
    16021 ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat tttgttcacg gaactgtttg
    16081 cggatccccc gcagtttcac tttcaattct gatacagttt gttcatatgg tgctaaaaat
    16141 gcttcccaat ctcgttccat agctgatacc tctacttcat taacattcat tgtttattct
    16201 accatatatg caaagttttt tcgtactctt gatttatgca ttgaccaaaa aaagaaaatt
    16261 aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa tgcttgaaat cgaatataaa
    16321 tctatgctga caaagcaaga gtacgagata ttcatcgctc actatcaatt aacagataaa
    16381 gatttccgtg tacagactaa tatttatttc gataccgttg atgaacagct gaagaaacaa
    16441 aattgtggcc tacggatccg tttcgttggt catcaagctg aatatacatt aaaaacacct
    16501 gctgaaaaag ggcgtctcga aacaacggat acctttataa cagaagaagc agaagcattt
    16561 atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc aaaaactttc tgatttgaat
    16621 atcgatcctg cttcattaat ccaaaccgga aagcttacaa caaaacgagc tgaattccct
    16681 attgaagaag gacttttagc tattgatgaa agctggggag acaatctaca cgattttgaa
    16741 ctggaactag aggtagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa
    16801 cgtttttctt taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa
    16861 aattagaaac ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta
    16921 agttatagga tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt
    16981 catatgatcg aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa
    17041 caacagttat tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa
    17101 tttcgctttc ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga
    17161 atttctcaga acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca
    17221 ttggactgca aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa
    17281 ttgcaagaag cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata
    17341 tttgaagaaa tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt
    17401 aaagaagtat tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct
    17461 tctgcagtcg tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa
    17521 ggtacaagta gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa
    17581 gtttttaatg gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat
    17641 ttacacttgc tttctaatta aaa
//
LOCUS       DASJST010000058        17649 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJST010000058 DASJST010000000
VERSION     DASJST010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.058"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(42..1181)
                     /locus_tag="U4B05_001500"
     CDS             complement(42..1181)
                     /locus_tag="U4B05_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN2050644.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1580..2878)
                     /gene="eno"
                     /locus_tag="U4B05_001501"
     CDS             complement(1580..2878)
                     /gene="eno"
                     /locus_tag="U4B05_001501"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN2050645.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2994..3749)
                     /locus_tag="U4B05_001502"
     CDS             complement(2994..3749)
                     /locus_tag="U4B05_001502"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN2050646.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3853..5043)
                     /locus_tag="U4B05_001503"
     CDS             complement(3853..5043)
                     /locus_tag="U4B05_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN2050647.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5153..6154)
                     /gene="gap"
                     /locus_tag="U4B05_001504"
     CDS             complement(5153..6154)
                     /gene="gap"
                     /locus_tag="U4B05_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2050648.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6195..7286)
                     /locus_tag="U4B05_001505"
     CDS             complement(6195..7286)
                     /locus_tag="U4B05_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN2050649.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7435..8769)
                     /gene="rpoN"
                     /locus_tag="U4B05_001506"
     CDS             complement(7435..8769)
                     /gene="rpoN"
                     /locus_tag="U4B05_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN2050650.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8829..8900
                     /locus_tag="U4B05_001507"
     tRNA            8829..8900
                     /locus_tag="U4B05_001507"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8862..8864,aa:Arg,seq:ccg)
     gene            complement(9092..9976)
                     /locus_tag="U4B05_001508"
     CDS             complement(9092..9976)
                     /locus_tag="U4B05_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2050651.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10063..10962)
                     /locus_tag="U4B05_001509"
     CDS             complement(10063..10962)
                     /locus_tag="U4B05_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN2050652.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10979..11485)
                     /locus_tag="U4B05_001510"
     CDS             complement(10979..11485)
                     /locus_tag="U4B05_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN2050653.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11674..12294)
                     /locus_tag="U4B05_001511"
     CDS             complement(11674..12294)
                     /locus_tag="U4B05_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN2050654.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12370..12807)
                     /locus_tag="U4B05_001512"
     CDS             complement(12370..12807)
                     /locus_tag="U4B05_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN2050655.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12970..13800
                     /locus_tag="U4B05_001513"
     CDS             12970..13800
                     /locus_tag="U4B05_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN2050656.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13784..15151
                     /locus_tag="U4B05_001514"
     CDS             13784..15151
                     /locus_tag="U4B05_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2050657.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15170..15979
                     /gene="yidA"
                     /locus_tag="U4B05_001515"
     CDS             15170..15979
                     /gene="yidA"
                     /locus_tag="U4B05_001515"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN2050658.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16022..16465)
                     /locus_tag="U4B05_001516"
     CDS             complement(16022..16465)
                     /locus_tag="U4B05_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN2050659.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            16599..16970
                     /locus_tag="U4B05_001517"
     CDS             16599..16970
                     /locus_tag="U4B05_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2050660.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(17139..17567)
                     /locus_tag="U4B05_001518"
     CDS             complement(17139..17567)
                     /locus_tag="U4B05_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN2050661.1"
                     /translation="MPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQPD
                     FTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERMNK
                     TKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
BASE COUNT         4839 a         3671 c         3202 g         5937 t
ORIGIN      
        1 aatgctaatc tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa
       61 ataattcctt gatatttttc gatcatatcc aaataattat cgatttcaac atactcatct
      121 acttgatgag gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta
      181 gctttcgtaa attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg
      241 ctgaattgtt gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt
      301 ttgttgtaat cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg
      361 attgattgca gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc
      421 ccttggagct gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta
      481 acattatgga ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt
      541 tcagcatttg ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct
      601 ggcatagagc tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg
      661 tgcgtataca tcaaactgta attggttggc tctccgatga tcaaggcatc taagtcatct
      721 acatagcctg ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg
      781 gctaataatt tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca
      841 atcaccattg ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc
      901 ccatggattt ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg
      961 acatccatat gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca
     1021 atcaaattat ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc
     1081 aaaagcttat tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg
     1141 atgatctctt gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca
     1201 tttcttttta ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa
     1261 tgaagaaaga gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata
     1321 cgagaggact tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga
     1381 tgctagttat tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta
     1441 aacatcatat acagaagcta gttgcaaaaa aactctctac ctgctgatat ttcagcaagt
     1501 agagagttcg attacttaac taagtgatca tactcgacta tctttcaata gtcgatcatg
     1561 tatcttcatt agaaaagaat tatttgtttt ttaagttgta gaaagatttc aaacctttgt
     1621 attctgcaac ttcaccaagt tggtcttcga tacgtagtaa ttggttgtat ttagcaatac
     1681 ggtcagtacg tgatagagaa ccagttttga tttggccagc gtttgttgca acagcgatat
     1741 cagagattgt tgaatcttct gtttcaccag aacggtgaga tacaactgct gtgtagccag
     1801 cttctttagc catttcgata gcttcgaaag tttctgttaa tgtaccgatt tggttcactt
     1861 tgataaggat tgagttagca attccttttt cgataccttc agataatttt tgagtgtttg
     1921 ttacgaacaa gtcgtcacca actaattgaa ctttgtcgcc taatacgtca gttaatttct
     1981 tgaatccgtc ccagtcgttt tcgtctaatc cgtcttcgat tgagatgatt ggatatttag
     2041 aaactaattc ttcgtagaat ttgatcattt cgtcagttgt tttttcgcct tcgcctgaat
     2101 cagctagtac gtaaacacct ttttctttgt cgtagaattc tgaagaagca gcatccatag
     2161 caagaacaac gtctttacca ggtacatagc cagctttttc gattgcttcg atgattactt
     2221 caaaaccttc ttcgtttgaa ccaaggtttg gagcaaatcc accttcgtca ccaacagaag
     2281 ttgctaatcc gcgagctttc aagattgaag ctaatgcgtg gaatacttca gcacccatac
     2341 gtagagcttc tttgaatgtt ggagcgccaa caggcatgat catgaattct tggaagtcga
     2401 ttgagttatc agcatgtgat ccgccgttga tgatgttcat cattggtgtt ggcaatactt
     2461 ttgtgttgaa tccgcctagg tagtggtata aaggtacttc taggtagtca gcagctgcac
     2521 gtgcaacagc gatagaaaca cctaaaatag cgttcgcacc taatttacct ttgttaggag
     2581 ttccgtctaa agcgatcata gctttgtcga tagccatttg gtcacgaaca tcgtagccaa
     2641 tgattgcttc agcaatgatg ttgtttacgt tgtcgacagc tttagttaca cctttaccgc
     2701 cataacgagc tttgtctccg tcacgtaatt caactgcttc gtattcacca gttgaagcac
     2761 ctgaaggaac cattccgcgg ccaaaagctc cgctttctgt gtatacttct acttcgattg
     2821 ttgggttacc acgtgagtct aagatctcgc gagcgtaaac atcagtaata attgacatgt
     2881 tttgtctctc ctttgagttt gttttttcct aagggatttc cctcatatac gattttagtt
     2941 aattttgctc attagtgcaa acatttcttg catttttcgc aaattttcaa caattatttt
     3001 acagcgtcta acaatgacaa gaatgaatct gcttgcaagc ttgcgccacc gactaaagca
     3061 ccgtcaacat tttcttttgc catgtattca gcaatatttt ctggttttac agaaccgccg
     3121 tattggatac gaactgcttc tgatacttct tttccataaa gtttttcaac tgtttgacgt
     3181 acaactccac aaatttcgtc agcgatttgt gcgtcagctg atttaccagt accgattgcc
     3241 cagattggtt cataagcaat aaccatgttt gacacctgtt cagctgaaag atcagctaaa
     3301 ccagctgtaa tttgtccttc gatccattca gcagttttgc ctgcttcata ggtttctaaa
     3361 gattcgccac agcataggat tggtgtcata ccatttgcaa agatagcttt cgctttttta
     3421 ttgatgtctt cgtctgtttc atggaaatat tcacgacgtt ctgaatgtcc gatgatcaca
     3481 taatgaacac ctagatcagc aagagctgct ggtgaagttt caccagtgaa cgcacctgag
     3541 ttttcccagt agcagttttg tgctgagatt tttagttctg taccttcagc tgatgtcatt
     3601 aattcttgta agaataatgc aggagatccg atgactgaat caactttgtc atttgaaggg
     3661 attttgtttt ttactgcttc agcaaattct tttgcttctg atgcagtttt gttcattttc
     3721 cagttacctg cgataatagg tttacgcatt gaggaacaca ctccttaatt tattgaaaaa
     3781 tggctaggga gtcatgaaaa ctcccttcac cttttgttcg attactcgtc agaattgagc
     3841 aaacttggtc gtttatttat cgttgattgc tgctagtcct ggtaattctt taccttcaag
     3901 caattccaag cttgcgccgc cacctgttga gatgtgtgtg aatttgtcag caaatcctaa
     3961 ttgttctgct gctgcggctg agtctccgcc accgatgatt gttgttgcgc cttctaagtt
     4021 agcgattgct tcacaaacac cgattgtacc tttagcaaag ttactcattt caaatacacc
     4081 cataggtccg ttccaaacaa ctgtttttgc accttgtaat gtatcagcaa ataattttac
     4141 tgatgctggg ccgatgtcta aagccatcaa accatcagga atgtctcctt cgattacttg
     4201 tgtttcaaca tcatttgaaa attctggtgc acagacattg tcgattggca agacaagttt
     4261 gtcgcctgct ttttcgatta attcttttgc tagttccact ttatcagctt ctactagaga
     4321 gttaccgatc ttgattcctt ttgcttcgta gaatgtgtaa gtcattccgc caccgatcaa
     4381 gattttatct gctttaggaa tcaagttttc gattacgccg attttatcag aaacttttgc
     4441 gccaccaagg attgcaatca ttggtcgttt tggttcttct actgcttcac cgatgaactt
     4501 gatttctttt tccatcaaga atccagcaac tgttgggata ccagttgaag cgatacctac
     4561 gttagaagca tgtgcacggt gagctgtacc gaaagcatcg ttaacaaata catcgcctaa
     4621 tgaagcccag tatttaccta gttctgtatc atttttgctt tcttttttac cgtcaatatc
     4681 ttcaaatcgt gtattttcga atacgacaac gtcgccgtca ttcatgttgc ggattgcttc
     4741 ttctaattgt tctccgcgag tttcaggtac gaatgttact tctttaccta ataattcgcc
     4801 tagacgtttt gcaactggag ctaaagattt tccttcttta tcttcttctg tttttacacg
     4861 tccaaggtga gagaaaagga ttgcttttcc gccttgttct agaacgtagt tgattgttgg
     4921 caatgctgct ttgatacgag tgtcgtcagt gatcacgccg tctttcaaag gaacgttgaa
     4981 gtcaacacgg acaagaactt ttttgtcttt taaatcgata tctttcactg ttttcttagc
     5041 cattttattt cctcctgaat aaacttatta tcaaaaaaag cggaagaagc gcgattgctt
     5101 ccccgctttt aaatgtacat gtctataatt tacacttgta cgtttgtcta tcttataagt
     5161 tagcgaagta ttctaaagta cgtactaatt gtgcagtgta tgacatttcg ttgtcgtacc
     5221 aagcaacagt tttaactaat tgtttgtcgc caactgtcat tactttagtt tgtgtagcat
     5281 caaataatga accgtaagtc ataccaacga tatctgaaga aacgatttcg tctgtgttgt
     5341 atccgtaaga ttcgtttgaa gcttctttca ttgctgcatt gatttcgtca acagttactt
     5401 ctttttcaag aactgttact aattctgtta atgaacctgt tggtacagga acacgttgag
     5461 cagctccgtc taatttaccg ttcaattcag gaattactaa accgatagct ttagcagcac
     5521 ctgttgagtt aggaacgatg tttgcagcag cagcgcgtgc acggcggaag tcacctttag
     5581 gatgaggtcc gtctagagtc atttggtcac ctgtgtaagc gtggatagtt gtcattaatc
     5641 cttcaacaac accaaatttg tcgttcaatg ttttagccat aggagctaaa cagttagtag
     5701 tacatgaagc acctgagata actgtttctt taccagttaa tgtttcgtgg tttgtgttat
     5761 aaacgattgt tggaacatcg tttccgccag gagcagagat aacaacgcgt ttagcaccag
     5821 ctgttaaatg tttttcagct gctgatttag aagtgaagaa accagtacat tctagaacga
     5881 tatctacgcc tagttcgccc catggtaatt cttctgggtt gcggttagct aatactttaa
     5941 cttcttttcc gtttacgttg aatgaacctt catgaacttc aactgttccg ttgaaacgtc
     6001 cttgagttgt gtcgtatttt aacaagtgtg ccaacatttt agcatctgtc aagtcgttga
     6061 ttgctactac ttcgattcca tctacatctt ggatacgacg gaatgctaga cgtccaatac
     6121 gtccaaaacc attaatacct actttaactg tcatttaaga tttcctcctt atgaaaatca
     6181 aaaaaatatt tattttaaag ggttacccct tttaaaatct catttgcagc agcttcatca
     6241 gtgatgagcc acgtttgttt tggtgcattt ttcatataag ctcgaattgc tttagctttt
     6301 gattttccgc ccgcgattgc catgatgatc gggacttttt ccaaatcttg caattctatg
     6361 ccgattctag ggaccttata aacgacttca cctttttcat tgaaaaagta gccaaaagat
     6421 tccgcaacag catttgcttg tttcagcatg actaattctt tttcagtcat cttacgacgt
     6481 gctgccatgt gtaatgcacg cccaatacta tgaataacac aattcgcttc actaatcaag
     6541 tttaagactt cttggataga tggctcattg agtaaagaat tgtaagttgc cagacttaat
     6601 tgttccggaa cataaagtgc acggtggtta ccgtttgctt tagcagccat tcttgcactg
     6661 acagaatttg cctgtacagt tatcgcttca ccaatcccgc ctcttgcagg gacaaacagg
     6721 ttatgccgtt tcttattttc cagattagac aattgttcgg ctaccacagc catagtagtt
     6781 cctcccatca cagcgatgat attctcgcct tctggaagta gtcggtctag ggcttcgctc
     6841 agcgcttcgc cgaatgcatc agccactttg gtctgctctt cgctgtttcc tgaaacaacg
     6901 atacagcgtt ggttaccaaa atattctgcc aactgttgtt cggtttgatg cgtacctaaa
     6961 agctgatcca tgaagttttc aaggctttga taaacttctt cgcccttagt tgtcagcgtc
     7021 ataccgcttt ttgaggtgtc gatcaaattc agatttttca aaagatctgt ttctgttcgc
     7081 aatactcgtt ctgtgatccc catactttcc gataaacttc gacgtccaat cggctgcatc
     7141 caatagatat ttcgtaaaat tttatagcgt tcttgcaaca cttcgataat atctggtgca
     7201 acagattcaa tcatttttat ttcatccagc atagcgatct ccttagttgg acactttacg
     7261 tccaatgtag acttaaaacg acccatatac gataaaaaaa gaaattaact attcgaattt
     7321 cacatcaata aagggaaatc tcaacctcac gactgtaagt ctaacagttt tgctctcgtt
     7381 tttcaactaa aaagactaga aaaagtgaaa tactttttct agtcttttga ctgattagtt
     7441 atcataacgt tttctttttg atgaactcgg gattcctaat aaatcacgat attttgctac
     7501 tgttcgtctt gagatcgcta tctcatcttt tttcagcaat tcaaccaatt tctggtcaga
     7561 caacggcttg tttttgtctt cttgatcgac taattcctgc agctttttct tcgctgtatc
     7621 tgctgaaagg tcttctgtct gttctgtact attcgtagga attcgtgtag tgaaaaattt
     7681 ttttaattca aatacaccga agtctgtttc caaatatttc ccgttgactg cacgactgac
     7741 cgtggattca tgcacatcta tctcagttgc aacttccttt aacgtaagag gcttgattgg
     7801 acgttccttg tcgataaaga aagctttttg ccgggaaaca atgacttgag caacccgcaa
     7861 aattgtatcc ccacgttgta ggatcgtttt cttcaaccat tcgaattcct gtttccgttc
     7921 ttttaaataa ttttcagttt ctttatctgc ttgttgtgac atttgtttga agtaactctc
     7981 ttgaaagcgt atatttggct gattttttcg gtttgagata acttgtacat ttttatcgtc
     8041 gtccactaat acccggacat caggaataat gtacaattcg ctgctacggt cgaaaatccg
     8101 gccagggctt ggactcaacg tttgtaaata gtcgaatatc tgctgaacag catgtagatc
     8161 aacagaaaaa cgttgtgcga tttctttcca ttttcgctcc accagttcct caaaacattc
     8221 ttccaacacg atataagcta attccggcgc ataatcatct cgttctgttt gaagcatgag
     8281 acattcttgc aaactacgtg cacctactcc agctggttct aattgctgaa tcagagtcaa
     8341 cgcatcgagc atttggatag gcgtagctcc aatctctttt ccggcttctt ctaaactaat
     8401 cgtcaaaaat ccgttcaaat cgatgtattc tacaagataa agaacgattt tcctcaaaaa
     8461 agtatctcgg taattcaaat ggacttgttc gatcaatgaa tcaaataaag acccttttgt
     8521 atcaggaatc tgatttaggt aatttgtctc ttctccttca taagaagatg aagaagcttt
     8581 tatgtaatct ggctgccatt ccggctcaac aacttccacc aatggatttt ccattgcttg
     8641 attctcaacg aatgccaaca gctcctcact gttatattgt aaaacttgga tggactgttg
     8701 caactgttgc gtcatggcta gtttctgtgt ctgctgctgt ttttgtgaaa aattttgctc
     8761 aaatttcatt tttttgtcca atccccttgt aattttttat ctgtttagtt acaattatcc
     8821 cagtgcttgc gtccttagtg tagtggatat cacgtaagat tccggttctt gagacggggg
     8881 ttcgattccc tcaggacgcg ttctactgct attttagcat aatttacgaa tagttgcttc
     8941 tttattttta agtgaaaact gtccgccaaa gaaaattggt ggacagtttt ttgttgtacc
     9001 tgtatagata ttgttaagcc tttgcaagta aggtagttat gtgttcaact acttattaaa
     9061 atgtgttatt tattaaagat acttgtctca cttactgaac tggccgatga tcgtttctgc
     9121 cataatagtt ctcgacagcc gttggatgcc ctaataaatc cccaactgtc gtgatgcgat
     9181 acccttgctc tttcagataa tcgatcactt gaggaacggc acgaatcgtt tcaggataga
     9241 tatcatggaa taaaataatt gccccatcgt atactgttgc ttggatcttc tgcaaaatca
     9301 aatcaggatt tcttgtcttc caatcttctg aatcaacaga ccattcgatg atcggtcgct
     9361 ggatgatcgt tgctcctaca ttcgtgacag aaccataggg cggacgaaca aaagtggggg
     9421 ttttgccagt gacttttttg atctcagtgt ctgctttcgt cacttcctcc gtcatcgact
     9481 gggcagaaag agtggccaga ttctgatgat cgtatgtgtg attgccaatc tcgtgccctg
     9541 cttcagctgt ctgcttcacg atttcaggat gctgcgccgt attttctcct aagacgaaaa
     9601 aagtggcatg cacgtttttt tcttttaaga ttcgcagaag ctgcggtgtt gtggtcgctc
     9661 ctggtccatc atcaaagctg attgcgacgc ttttgacagc cggatctcgg ctcgtttttg
     9721 tactgtcttg tgttgattgt gatgactgac tttgtacctt ttccgctgat tcttgaatgg
     9781 cttctttgac gttagctgtt tcagttgatt ggcttttaat ggttgattgt tctgattgtt
     9841 tgaaagattg tgacgaatgc tccgaagagt gtgtttgtcc ttttacttcg gtgatcaagg
     9901 aggccaccga agtaatttcg aaaaacaaaa gcacggataa agcaataata ctgataacta
     9961 aataatttct tttcactata tctcttcttt ctctttttct cttcttatta tttacttgta
    10021 cttacttgaa aattgaaact gctagtcgca actacttctc gtctaatgat ccaaataatc
    10081 ttccgatgcc aaaatcgttt ttgtcacgga cttaggaatg acaaattctg gtgtacccat
    10141 ataaccagga gcaatttcgt attgagaaaa agtcaaaact aattgatgct ctttgttcaa
    10201 ataaaaactt cgtgtaggcg taacaagtgt tggtttttct atcgtccctt cttgaatatc
    10261 ttgctcagtc caatagtact tagatggatc ttccttgact tgttcggcca tctgccgttt
    10321 gacttctttt gtcaaaacag atagataggt gtcatctttg aataaaagtg gaagtgagag
    10381 ctggacactg tttttcttgt cgattgtgtc gtatcttttt tcaacatggc tatctgctgc
    10441 tgtttgggtg atctgccttt caatcacgag aaaccggtta tcgtctacga ttttctgata
    10501 atctcccaaa acttggtaat gatccgtttc aaaatttcca tattcttttt cagctttttc
    10561 aaaatctgct tgtccttcgc gaaagtattt ttgatttaat ccatgaattg ttttattttc
    10621 tttctcattt tgtgtattga tcttcggtac ctgaatatcg attttttcag atcgatcagt
    10681 aaagcgttcg cctaagatca attcaatgac tgatcctaag atgggtaaat cttctgcaaa
    10741 gcttcgaact tgactattaa aaagaaggct acttgttgta atcattacga tagccaaaga
    10801 tactccgaac ccttggagac gtctctttct ccgctttgct tttataaatg attgctcttc
    10861 gataataaaa cgctggcgga tctcctcttt aagccctttg ggaattgctt gttctttata
    10921 agttttcttt agttttttta aattttcttt ttgggaatac atggcactct tctctccttc
    10981 attctaggat ttgacgtaaa gcattcaatg agcggtacat tttcgtttta actgtgtttt
    11041 cattcatatc caagatagtc gcaatttctt tcaaacgaaa tccttcaaaa aagtagagga
    11101 tcagaatctc tttttccgga ctgtctattt tatctaaaat catatggagc tctgcaacgg
    11161 aagtttctaa aggtaactgt tgggggatat cttgaatttc aaataagtcc acaggcgtcc
    11221 ttttttgtct gcgccaacta tctaatgccg tgcggatcaa tatccgataa aaccatgcac
    11281 taaaatgctc gatctctttt tctttttcaa aatagttcag ggctttatga aaactatctt
    11341 gcatcacatc taaggcctct tctttatcat gtacgtaatt catcgtgata cggaacattt
    11401 tttgataatc agtttcgatg agtccattca gcaggtgacg gtatttttcg atctgccgtt
    11461 ttttcttata tccagtaaaa atcattctcc actcctttct gcctcactta atatgacgta
    11521 caaggtgcgc taaaagtttc aattttttta ttttttatgt atttacttgg gatttttata
    11581 tacaaaaaag aaggtgtgac aaaaatcgtg tcacacctcc ttattctgat aggctgatcg
    11641 tctctgtcac aacctctcgt aagaaaataa tgtttattct ttttcagagt cagaaaattc
    11701 ttcctcttca taatcttcat catcatcgtc ttcgtctatg atacttaaat cttcttcgat
    11761 atttcctggc aagtcttctt catcatctgt cgggtcatct gctccgattt cttgcaagtc
    11821 agaattataa gcagcgattt cttcatcttc atcatcatcg tcatcaaaca gatcatcgtc
    11881 atcttcttct gtcagatctg tatcttctgg gtcatcgtca ttgtaatcga tcgcgtcttc
    11941 atcatcattg ataaaggcgt tgactttctt gcgtttacgg cgacgtggag tatcttcatc
    12001 ctcttcgtcc attccatggt ttacttcttc atcgatcgaa tcgatagcat accatgaacg
    12061 caagccccaa cgattatccc ctaatgagat aaagctgccg tcaatattca agtcagtata
    12121 aaattgtgcg agtgaatcac gaatttcact gtctgatttc ccaagatagt tttggatctg
    12181 gttagctaaa tcagaaaaat ccatgacatc gccacgttgt tctaagatcg cgtgtgctac
    12241 ttcgatcatt gataattcgc ttttgtttgc accttcaaat acactaattt ccaatcagga
    12301 cacgtccttt cacagtctac tccttatcat acaaaatcag acacaaaaaa tcaaactttt
    12361 cttttatatt tacgctgtaa aatccaaggt caaataatat tcgccgattt tttccggtcc
    12421 catgaacaga tcataatcga ttcgaacctt ccctgctgtg ggacggtctt tcaaactaac
    12481 atgcagttct ttcgtaaaag tagcaatctg gatcataccg tatggtgttc gataggatgt
    12541 ttctaaccgt tctttatagc caaatttcaa acgcatccgc atttctcctg cacggatcag
    12601 ctgtacatga ccatctggaa ctaatttgat cgttacagga atctcatcgc ccgtctcctc
    12661 ctgaatctct ttataacgta tgtacagcgt atcgccaatt gtcaccattt gccctttcaa
    12721 atcaaaataa aaatcttgca cttccccttg ctgttttact tttgtacgca atttgattga
    12781 aatcggtgtt ccttgtgata attccattat tcattctcct ttacttctca tagctacatt
    12841 gttcctagtt tcttttgttt tgtcaaccaa cttgtccatt tttacagaaa accttttttc
    12901 caaaacaact ctttgatttt agagtataat attgtagaaa atatgaattt atacaaagga
    12961 gccggttgaa tgaaccctgc gattcgacta gatcagcaca tttatcaaaa aaacgacttt
    13021 tccccttttg cattaacaga tatattaacg gaatattcta aagaacacca gcaaatcttt
    13081 ttacatttct ggcaatatga ctgcacagtc atcttaggta tgaaggatgt tcgaactcct
    13141 tttctaagtg acgggatcca aacactgaaa gatgcaggtt acgctccagt gatcagaaat
    13201 tcaggtggat tagggatcgt atcagatgaa ggaattttga atatttcttt gattttcccc
    13261 caaagtgaca caaaaaaaat aaccatagat gacgcttacg agcaaatgct cctattgact
    13321 aaatacgctt ttccaaaggc accgatacaa gcattcgaaa tcacccattc ttattgtcct
    13381 ggaacttatg atttaagcat ccatggcaaa aaatttgccg gtatcgctca gcgtcggatc
    13441 aaaaatggga tttctgttat gatgtactta agtgtcaacg gtaatcagga aaaacgcggg
    13501 caagtcatga agcgatttta tcaatctgct ttaaaagaag actttggtac aaatggctat
    13561 cctgcagtgg atcctgctac aatggcaaca cttgaagaac tgcttgacca accccttttt
    13621 gtaaaagaag tcaaagaagc atttgcaaaa gcttttgaaa agctgtattc tgcaagtgat
    13681 ttacaggatt ctaccgactg gctaaaaaaa gaaatccagc tggagcaatg gaacaaccaa
    13741 atccagagaa tggaacaacg taataacata aaggagctac attatgacaa ccccttataa
    13801 atatcaaatg ctgcctatgg aaaaagtttt tcgtgatcca gtccataact atatccatgt
    13861 ccagcatcaa gtaatcttag atttgatcaa ttcgaaagaa gtccaacgtc tacgacgcat
    13921 taaacaatta ggcacttctt ctttcacttt tcacggagca gaacatagcc gttttactca
    13981 ttcattaggc gtttatgaga tttcaagacg aatctgcgat atcttttctc gaaatttttc
    14041 aaaagaaaaa ataggcaacg gaggctggga tgatcaggaa cggctggtga ctttatgtgc
    14101 ggcactgttg catgatgtcg gtcatggtcc ttactctcat acatttgaac atatcttcca
    14161 tacgaatcat gaagccatca ctgtagagat catcacttct ccagaaacag aagtgtatca
    14221 gattcttaac cgagtagaag aaggttttcc tgaaaaggta gctagtgtga tccaaaaaac
    14281 ttaccccaac ccacaagttg tccaaatgat ttctagtcag atcgatgcag atcgaatgga
    14341 ctatttgctt cgggatgctt atttcactgg aacagaatat ggaacatttg atcttacccg
    14401 aatattacgt gtgattcgtc cttatgaagg cgggatcgct ttttccatga gtggcatgca
    14461 tgcagtggaa gattatatcg ttagccgata tcaaatgtat gtgcaagtgt acttccatcc
    14521 tgtttcccgt ggtatggaag taattttgga gcatctgctt catcgcgcac acgagttgta
    14581 tcaagaagat gccagcagtt ttgaactcca ttctcaatta cttctgccat ttcttgacgg
    14641 aagctttact ttagaagaat acctcaaact tgatgatggg gtcttgggca cttactttac
    14701 tcaatggtct gaatctgctg atcctatttt aaatgattta gctaaacgtt ttttgaatcg
    14761 taaaccactg aaatctgcta ctttttctgg caaccgggat tcaaaattag tccaagaact
    14821 cactttgtta gtagaaaagg tcggatataa tcctgtttat tacactgctg tgaattctag
    14881 ctacgatcta ccttatgatt tttatcgccc agaacaaggc agacatcgca cacaaatcga
    14941 gattttgcga aatgacggaa ctttgataga attatcacag gttagtcagc ttgttgctgc
    15001 attagcggga caagaacaag gagatgagcg atttttcttt ccaaaagaaa tggttgatcc
    15061 gtccttgcgt gatcattatg atttatttga cgaaacttat caagaatttg ccagccatat
    15121 ccgtaatggc gcattgattg aaataaatta aaatatattg gaggaaatta tgtctatcaa
    15181 actagtcgct atcgacatcg atggtacttt attaaatgag aaaagagaag taacaaaaga
    15241 agtaaaagaa gcgattgccg ctgctgttgc aaaaggtgtt tcgatcgttt tatgcactgg
    15301 ccgtccctta ccaggtgtcc aggaacagtt aaacgagttg aacctttttc aagataatga
    15361 ttacgttatt acttataacg gggcactcgt acaacaaacg aaatcaggta aaatcatcgc
    15421 ccgtcacgga ctgacacatg aagatttcct agagatcgaa gtgatggcac gccgtgtcgg
    15481 cagtcatctc cattcgatcg atgacaaagc gatctacacg cctaatcgag atatcagcgc
    15541 ttatacgatc cacgaagcaa gtttagtcaa gatgccgcta aaatatcgaa cacctgaaga
    15601 aatgacacca gacatgaaca tcgtcaaaat gatgatgatc gacgaaccag aaattttaga
    15661 tgcagcaatt gcgcgtttac cgcaaacttt ccgtgataaa tacacaacgg tcaaaagtgc
    15721 gccctattac ttcgaagtac taaataaaga agcaagtaaa ggtgcggcag ttgcgaatct
    15781 cgcacagcac cttggtattg atcaagatga aatcatggct atcggagata acgagaacga
    15841 cttatccatg attgaatatg ctggtctagg agtagcaatg ggaaatgccg tttctcttgt
    15901 caaagaagca gcaaatgtca tcacttcatc aaacgacgag cacggtgtag ctgaagcaat
    15961 caaaaaatat gtactgtaat aaataaaaaa actgagagaa agctgatcta ctcgctttct
    16021 ttcagttttt atttttttcg atccatctct cgactgccca tcggtggcta ccacgtttca
    16081 atttcacaag cgcttcttga gggctcaccc accaaatttt attggtcttt tcagttggtt
    16141 ctcctatacg cttccaagaa tcagcaacaa agaaatatcc tggattataa taataagttg
    16201 cacgataatt cgagtaaaaa tactcatcag cttctcccag ataagaagaa atttctgcca
    16261 caattcccat ttcttctatc agctcacgga caatcgcttc ttctttcgtc tcttctcctt
    16321 caatttcccc gccaggaaga aaaaaagcac catttggagc ctcgattact gcaatctgct
    16381 gatctctttc tatgacaata tatgctgcat aacgggtttg ataatcagca aattgctttt
    16441 tttcaccaaa aattggttta tccatgaata gtttccacct taacagtttt attttctatc
    16501 cattttacat gagaaatttc tcatgaacaa gcacaaatca actatttcct tacttctttt
    16561 tgttatgatt gagtaaaagg aaaaggagag atgaatctat gaaaatggct cacacatgtg
    16621 ttcgagtgaa agatcttgaa gcatcaatcg aattttatca aaaggcattt ggttttgaag
    16681 aaagccgccg ccgtgatttt ccagaaaaca aatttacact tgtttatttg acacttcctg
    16741 gggatgacta tgaattagag ttgacttaca actatgatca tggtgcgtat gatctaggag
    16801 acggctatgg tcatatcgct atagcagctg atgatctaga aaaattacac gagcaacata
    16861 aagctgctgg tcttgagatt actgacttaa aaggattacc tggcactgcg ccttcttact
    16921 actttgtcat tgaccctgat ggttataaaa tcgaagtcat ccgaggatag ttagtatggg
    16981 gcattttact cttgattgaa taaaaaacaa acaccaataa ttaaaatgag acgcaaactt
    17041 ttcattaaat tgaaaaagtt tgcgtctttt tttaacaacc attgtagcta taaggttgcc
    17101 actctccacc aatttatttt taattcattt gaaagaaatc aaataaacga ggtaccttta
    17161 gaaaacaaaa ctgcttttcc tcctattatc acacgatctt cttttaattc acattctaga
    17221 tccccgcccc ttgatgacat ttgataagct gacaattttg ttttattcat tctttctgcc
    17281 caataaggaa taagattaga atgtgctgat ccacatactg gatcttcatt gattgttaat
    17341 tttggaaaga aggttctaga aacaaagtct ttttgcacac tttctgctgt cacgatgact
    17401 cctattccta attcaaaatt tttgatagct gtaaaatctg gctgcaattt agccaccgtt
    17461 ttttcatctt ctaatacgaa aaataaatca cgtgctaagt aggcttcttt aatcttagcc
    17521 ccaatcgctt cttcatattc agacaatatc ggcacttttt tcggcataat actaggaaaa
    17581 tccatgtaat aataatcttt aattcttgtc acaaatagat taccgctttg agtagaaaat
    17641 ttaatcgtt
//
LOCUS       DASJST010000059        17006 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJST010000059 DASJST010000000
VERSION     DASJST010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.059"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..125)
                     /locus_tag="U4B05_001519"
     CDS             complement(<1..125)
                     /locus_tag="U4B05_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN2050662.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="U4B05_001520"
     CDS             complement(229..540)
                     /locus_tag="U4B05_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2050663.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="U4B05_001521"
     CDS             complement(542..739)
                     /locus_tag="U4B05_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN2050664.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="U4B05_001522"
     CDS             complement(1133..2860)
                     /locus_tag="U4B05_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN2050665.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="U4B05_001523"
     CDS             complement(2853..4046)
                     /locus_tag="U4B05_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2050666.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="U4B05_001524"
     CDS             complement(4233..4877)
                     /locus_tag="U4B05_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2050667.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="U4B05_001525"
     CDS             complement(4971..5105)
                     /locus_tag="U4B05_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN2050668.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="U4B05_001526"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="U4B05_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN2050669.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="U4B05_001527"
     CDS             complement(7236..7709)
                     /locus_tag="U4B05_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN2050670.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="U4B05_001528"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="U4B05_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN2050671.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="U4B05_001529"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="U4B05_001529"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN2050672.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="U4B05_001530"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="U4B05_001530"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN2050673.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="U4B05_001531"
     CDS             complement(11756..12895)
                     /locus_tag="U4B05_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2050674.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="U4B05_001532"
     CDS             complement(13204..13917)
                     /locus_tag="U4B05_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN2050675.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="U4B05_001533"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="U4B05_001533"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN2050676.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="U4B05_001534"
     CDS             15106..16638
                     /locus_tag="U4B05_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2050677.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="U4B05_001535"
     CDS             complement(16829..>17006)
                     /locus_tag="U4B05_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2050678.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5323 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DASJST010000060        16980 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJST010000060 DASJST010000000
VERSION     DASJST010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.060"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..649)
                     /locus_tag="U4B05_001536"
     CDS             complement(20..649)
                     /locus_tag="U4B05_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050679.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1100..1984)
                     /gene="rsmA"
                     /locus_tag="U4B05_001537"
     CDS             complement(1100..1984)
                     /gene="rsmA"
                     /locus_tag="U4B05_001537"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN2050680.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(1977..2546)
                     /gene="rnmV"
                     /locus_tag="U4B05_001538"
     CDS             complement(1977..2546)
                     /gene="rnmV"
                     /locus_tag="U4B05_001538"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN2050681.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(2539..3315)
                     /locus_tag="U4B05_001539"
     CDS             complement(2539..3315)
                     /locus_tag="U4B05_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2050682.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(3470..4792)
                     /locus_tag="U4B05_001540"
     CDS             complement(3470..4792)
                     /locus_tag="U4B05_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2050683.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            4967..5554
                     /locus_tag="U4B05_001541"
     CDS             4967..5554
                     /locus_tag="U4B05_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050684.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(6033..8042)
                     /gene="metG"
                     /locus_tag="U4B05_001542"
     CDS             complement(6033..8042)
                     /gene="metG"
                     /locus_tag="U4B05_001542"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN2050685.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            8310..8588
                     /locus_tag="U4B05_001543"
     CDS             8310..8588
                     /locus_tag="U4B05_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050686.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8685..9506
                     /locus_tag="U4B05_001544"
     CDS             8685..9506
                     /locus_tag="U4B05_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2050687.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(9791..10801)
                     /locus_tag="U4B05_001545"
     CDS             complement(9791..10801)
                     /locus_tag="U4B05_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2050688.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(10813..11766)
                     /locus_tag="U4B05_001546"
     CDS             complement(10813..11766)
                     /locus_tag="U4B05_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2050689.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(11756..12826)
                     /locus_tag="U4B05_001547"
     CDS             complement(11756..12826)
                     /locus_tag="U4B05_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050690.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(12832..13875)
                     /locus_tag="U4B05_001548"
     CDS             complement(12832..13875)
                     /locus_tag="U4B05_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050691.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(13876..14817)
                     /locus_tag="U4B05_001549"
     CDS             complement(13876..14817)
                     /locus_tag="U4B05_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050692.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            14901..15248
                     /locus_tag="U4B05_001550"
     CDS             14901..15248
                     /locus_tag="U4B05_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN2050693.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(15318..16979)
                     /locus_tag="U4B05_001551"
     CDS             complement(15318..16979)
                     /locus_tag="U4B05_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2050694.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4844 a         3504 c         3038 g         5594 t
ORIGIN      
        1 tttttttgag agatcatgtc taatatgtcg ttttcttcgc gtacccttga atcgactctt
       61 tccgattttc tttttttgct gccgtaaata cggggatatc aaaatatttg ctgagtgttc
      121 ctgctataaa ataagcaata ttataatcaa ctaaactgtg gataagccca ccaatcccca
      181 tcaagatgaa tactgtataa aaatatcctt gagaataata tgtttcaggc atattaccaa
      241 taaagtaaaa gatgctgact actagcattt ctgctaaaga atgaatcaca ccgatcacaa
      301 aattgaaccc ttggaaacgc caattagcaa aagtaaactg tccatttttt aaaacgatac
      361 tcggatgctt ttgcaagtac caagccccaa tcaacgcaaa cacaagatgt gacaatgctc
      421 gcaatgcgat gataaccggc gttgttaaaa agaaaccaaa tgtcgtacct agcgcaacag
      481 ccactgcaac tcctggtgta aaaaacatcg caataaataa tggcacatga gacgccaaag
      541 taaaggaagc tggtggaatc atgattttag gcatgaccat tgggataatg acccccatcc
      601 caatcaataa agcagaaatc gttagttttc gaactgaatt ttttctcatt ttttctctct
      661 cctaatatgt cttgacacat aaatgtcgac ttagtataac tcacttacta ataactgtca
      721 agacacatat tcttttctaa atccatacaa aatgagtgtg agtatcaagt ataagaattt
      781 tttgatcaag aaacaaaaat aggagatgtg acaaaatttg tgtcacatct ccttgttcgg
      841 cataatagtg gtcaaaagct tgcatcaagt aataagacca actaactcaa aaacatgaga
      901 agctgttttt tgaaattgtt ccttgtgact tagagttaaa cggcaggccc acaacctctt
      961 tcttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaacaaa cgaaaaacat
     1021 gaggaactat ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacaact
     1081 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
     1141 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
     1201 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
     1261 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
     1321 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
     1381 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
     1441 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
     1501 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
     1561 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
     1621 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
     1681 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
     1741 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
     1801 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
     1861 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
     1921 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
     1981 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
     2041 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     2101 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     2161 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     2221 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     2281 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     2341 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     2401 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     2461 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     2521 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     2581 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     2641 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     2701 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     2761 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     2821 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     2881 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     2941 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     3001 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     3061 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     3121 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     3181 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     3241 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     3301 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     3361 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     3421 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     3481 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     3541 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     3601 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     3661 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     3721 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     3781 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     3841 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     3901 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     3961 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     4021 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     4081 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     4141 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     4201 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     4261 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     4321 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     4381 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     4441 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     4501 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     4561 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     4621 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     4681 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     4741 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccatcc
     4801 tctttcttct ttgaaatttg gtataatttt cagtataact gagaatattt taatttacca
     4861 ttcttttttc tccactttca gtgaaaaact ccctcttaag acggatgtat ttctttttta
     4921 tttttcgtat actaaaccta ccttttccac gaaggaggaa cagtttatgg attttaagcg
     4981 ttcttatccg aaattagcag attatttatc caaccgggtc gatttttccc gtaaagaaca
     5041 acagcagtcc cattggcatt ggttcacatt aattttattt ggattaatga ctgttgcaac
     5101 gttatcacaa aaactaatat tgctgacttt gttcatcgca atcgttgcat tgatcaaagg
     5161 tcctgtgatg atcttgtggg ggatgttgta ttcctttttg gttgggcttt ttccgcctat
     5221 cggtattttg ctttcagcga ttttcttttt attgaatatc ggaacattta caaagaactg
     5281 gcggatgacc ttagtcggga tctattttta tttctatcct tttggagtga tggcacttag
     5341 tgaagtcatg cattgggata atcattggtt tatagctggc tccttgcttt tagggttgat
     5401 cttgttacac gtgatgttga caaagctcta tcaacattat ggtatcggac gtacgatttt
     5461 ctggtatgtc ttttcgatcc catttgcact gcttactgct ctcttaccaa gccgtttgaa
     5521 gacaaaaata aaaggttacc ataaaatcaa gtgataggat ttttacaaaa taaagaaaac
     5581 aatcatcgaa gtcaaagccg atgattgttt ttttatctat attttcaatc acacatataa
     5641 gagcacaaac taattgaaca taaagtgata gaacttataa ttaacacatc aacgctactg
     5701 ttagatataa atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca
     5761 ataaaaaaga gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt
     5821 ttttctgttt ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc
     5881 gtacttcttt ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc
     5941 ttcaagtaat aagaaggaat ttacgaaaat tgcttctcaa atttcggaaa aagggggtat
     6001 gacacaacta gtgtcatacc ccctttgaat ttctatgcga tttctgagcc atttggcatg
     6061 gattttggtg cttcgattac ttgtaaagag ccatctggtg cttctgcaga aaggatcatt
     6121 ccttgactga tttgtccacg catctttcgt ggtttcagat ttgctacaat gacgactttt
     6181 ttcccgatca attcgcttgg atcaggataa aattcagcga ttcctgacaa gatttgacgg
     6241 tcttggctgt ctccagcatc tagtcggaat tgcagcaact tatctgcccc ttctactttt
     6301 tgacagttga tgacttctgc tacttttagt tctacttttt caaatacatc aaatttgatt
     6361 tgtttttctt tcgttgaaac aagttctgtt tcttctggat cccatttgac tgtatcttcg
     6421 tttgtttgag taccttcaga cattttttct tgaataaaag caacttcttc ttccatatct
     6481 aaacgtggga agattggtgt accttttgca actacttttg ttcctgaagg aaattcgcca
     6541 aagtgtaatc cttcaagatt cattgtttca gagtccaatc ctagttgatt gaagatcttc
     6601 tctggcgttt ctgtcatgat tggctgaagt aatatcgctg cgatacgcaa agattcagct
     6661 aaatggatca tgacactatt caattcagct ttcttttctt cgtcttttgc taatacccat
     6721 ggttctgttt catcaatgta tttgtttgca cgagaaatca gcacccagat ttcagccaat
     6781 gctgtattga attccatttt ttccattgca tcatggtaac gtccgacaac gttagcagcc
     6841 gtcgtagaca gttcactatc aaatggagtg acaagtgagg catagtcagg aacgagtcca
     6901 tcacaatatt tgttgatcat agcaatcgtt cgattcaata agttccctag atcatttgcc
     6961 agatcataat tgacacggga aacaaaatct tctggcgtaa agacaccatc tgacccaaac
     7021 ggaacagcac gaagcaaata ataacggagt gcatctaaac cataacgttc cacaagcatt
     7081 tcaggataaa cgacatttcc ttttgattta gacatttttc cgtctttcat caatagccag
     7141 ccatgcccaa atactttttt aggtagaggc aaatcaaggg ccatcagcat gattggccaa
     7201 tagatcgtat gaaagcgaac gatttcttta ccgaccatct gaacatctgc aggccagtat
     7261 ttttcgaata gactttcatc ttcggagcca tatcctagtg cagtaatata attggataat
     7321 gcatcgatcc atacatacac cacatgtttc ggatcactct tgactggtac tccccaagta
     7381 aaagttgttc gtgaaactgc aagatcctct aatcctggtt tgataaagtt gttgatcatt
     7441 tcgtttttac gagattctgg ctggatgaat tcgggatgtt cttcatagta ttccaataga
     7501 cggtctgcgt atttgctcat gcggaagaag tatgattctt cttttaccaa ttctacttcg
     7561 tgtccacttg gcgctttacc accgatcacg ttaccttcgt catctttgta cacttctgca
     7621 agttgtgttt ctgtaaagaa ttcttcgtca gaaacagagt accagccttc atattcacct
     7681 aagtaaatat ctccctgttc taataaacga tcaaatattt tttgaacagc tgctttatgg
     7741 taatcatctg tcgtgcgaat gaatttatca taactgatat caagcgtttt ccataatttc
     7801 ttgacatcag ccgccatttt atctacatat tcttgcggtt ttacacctag ttcttctgct
     7861 tttgtttcga ttttttgacc atgttcatcc actccagtga gataaaacac atcaaagccc
     7921 atcaaccgtt tataacgagc aattgcgtca catgcaatcg tagtatacga gttgccgata
     7981 tgaagtttgc cgcttggata atagattggc gtagtgatat aaaaagtttc cttttctgcc
     8041 acggggctct tcctccttta tcgcatgatg cgaattcgtt tccttgctag tataccataa
     8101 aaaaagcatg ctgttagctt tttttcaaaa tgaaaaagac tggataaacg acttctatct
     8161 acgttcaatc tatcctttaa aaggatgaat aggttgaata aatgcgggtt tttgttttgt
     8221 ttcatatcta ctaaaaaacg gctttttatt tctcccctca cacgtggtat aataaagact
     8281 atctatctta gtaagggact ggaacaatca tgcagaagaa acatcttttt tttacactta
     8341 gtattgcttt tctttcatta gcacatttaa tattcagcta tttttatatt cgtatgtatg
     8401 gttattttaa cctccatggg catttgaaca gttttatgac agcagcttgg attttgcgtt
     8461 ttatcattga cgtatatatc gttatttgtg gcttctttgc cattcgtgaa gagcggtata
     8521 aagtattgcc gttttatctg cttttctttt tattcaatct catacttcca tttatttttc
     8581 atatttaaat gataacctag atttttcaag taaatccttg gttttttttc agagtctgct
     8641 acaataaggt aaaaagtact tttactgtca atggaggtgg tcagatgaac cgttttgctt
     8701 ctaaagaaga agaaaaatta tattatgaac ataaagcaag tgaagaagaa tttcttaact
     8761 ggtatcaaaa acaggaacgt ccagaatatg acaagccctc tttgactgtc gatatcgtac
     8821 tgatgtgcta taacaaagaa gaagatcagc tgaaagtttt gttgatccaa agaaaagggc
     8881 atccttatcg aaattcatgg gcattgcctg gtggctttgt ccaaaaggat gagtcaactg
     8941 gagaaagcgt cttaagagaa acaaaggaag aaactggtgt ggttatttca aaagaaaata
     9001 ttgagcaact gcatacattc agtacgccta atcgagatcc gcgaggctgg gtcgtcacag
     9061 tcagctactt agcctttatt ggagaagaac cgctaatcgc cggagatgat gccaaggaag
     9121 tacgctggtt cacactagaa cgtcatagta acaagatcca tttatctagc ggagaagtgg
     9181 ctatctcgct tgatttagtc actggagagt cttctggcaa agatactttg gcttttgacc
     9241 atagtcaaat catcttgaaa gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc
     9301 aagttctaca agttttaggt aaagatttca cgattacgga agctcgaaaa gtatttgcta
     9361 aattcttagg gatcgattat aaaacgatcg atcattccaa ttttaagaaa gctatgctcc
     9421 aatatttcga tgagatcggt gaacgtcctg taggaatcgg ccgtccttca aaaatctatc
     9481 agctcaagcc ggggcaccat gaataaggtt gtgagcaaaa gattcagacc taatcattca
     9541 agaaaaaacg caaaaacaac tctttgtgag ttacgttttt tgtttcagaa ttttgatgta
     9601 ttcgtaaact ttctgaataa aaaattaaca atagcagaaa aagctcgtac gaactttttc
     9661 tgctattgtt ttattggttt gtcataatta gtgagctttt cttcaaaaga aaaattccta
     9721 gaaacaaaag tcattaagac tttttattaa caaggctaac cctaacgctc ctatcagttt
     9781 cattcatttt ttatcccttg ataaccgaat gtctgcccat tttttctact acttggctct
     9841 ttcttacgga attgctttct tctgctccgt cggtattgat caaaagaaat tttttattat
     9901 accattggga cttataacgc aattttgtta ttaatttatg ccagtcgtga atagaaaaat
     9961 cagcatgtgc ctcttctttt ggaaaagatt ctacaaataa gtagtcatat tgtttgacca
    10021 atttcccaga taattgttca agataatctt ctcgctggtt cttttgtttc agataaagat
    10081 ccattacttt tctcttttgt ttctgataat tgcttgcctg atctaacctg atctttctat
    10141 ggtgagcgga gcggactttc acattcaatt tccgctgtgc atgttgcagc ttttcttctg
    10201 tctggtgaaa ttttggtaaa gttacttcta gaggtcgaga tgtttggatc aatgtcttcg
    10261 gatgataggc gatcccgatc catttagcta aagattcttt ttttactggc gaaacatcac
    10321 aaagtagtgc gacataaaat tcttcattat atcgagcaga aatcgtagcg gagcgaatcg
    10381 atccttcaac tggccgatgc tgatttatct tgattagttc tttttgtttt ggtaacttta
    10441 agtagccatt ttccagatag atcgtacggg tttggttgtt ggtcgtataa gattgccaca
    10501 tacttttttt cgttttaagc tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag
    10561 cccgatccaa attaagctgt gcatttgcta aagcaagact gtctacttct tttaagaatg
    10621 ggtattcttt ttttaatgta gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc
    10681 aacttttctg ccgctttttc agcaaatggt tataggtaaa tcgagcacat ccaaaagtac
    10741 gaatcaattg ctgtttttgt tcttctgtcg gatacatacg gaatttaaaa gcttttagca
    10801 tctatgtccc tcctagttag caaccagaag agattccttt tgtcgtttta gtttttttgc
    10861 gtaatatggg acttcctgct cagttgcata gacaaaatgt tctggtgcga tttcacgaag
    10921 ttgacgtact tgtccgtcat ctggttcagg ttgatatttg attcttctgc gttttctctc
    10981 atggtctgga tctggtagag gaatagcaga taacaaactt tctgtataag gatgaacacc
    11041 gtgattatag atttcatcac ttgttcccat ctctaataac aagccgttat gcattacacc
    11101 gattcggtca ctaatatgtt taaccatcga caaatcatgt gcaataaata gataggtcaa
    11161 ttgatgttct ttttggagat cttgcaacaa attgaccact tgggcttgga tcgacacatc
    11221 tagtgcagaa attggttcat cacaaatgat aaaacgcggt tttactgcca atgctcgtgc
    11281 aataccaata cgctggcgtt gcccacctga aaattcatga ggataacgag taccgtggct
    11341 tggattcaat ccaacagttc tcaatagatc atcgactttt tttgcacgct cttcagatga
    11401 tttagctagt ccattaacat caattccttc ggcaatgata tctctgactt tcatacgagg
    11461 attcaacgaa gcgtatggat cttgaaagat catctgaaca tcatgccgga atttcgtcaa
    11521 acctttttta tctttgatct tcgttatatc ttgaccgtcg aataatatct caccatcagt
    11581 aggttcattc aagcgaatga tcgtacggcc agtagtggat tttccgcttc cagattctcc
    11641 aactaaaccg aatgtttctc cttcataaat gtgaaagctg atgtcattta cagcatgtac
    11701 ttcattcttt tttcctacat tgaaatactg tttcaatcct ttaacttcaa gtagtttatt
    11761 tggcatcagc aattcctcct tgaccgtaag tatgtttttg tttctcttta aagatagccc
    11821 agcgacgttg gatttcttct ggcggtgtca ctttgggtgc ctgcggagca agtagccaag
    11881 ttgctgcctt gtgtgttttt gatagttcaa aaaatggtgg ttcttgttct gcatcgattt
    11941 tcatcgcgaa ttcatttctt ggatagaatg cgtctccttt tggcgggttc aataaatccg
    12001 gtggagaacc aggaatagca tacaatcgat cgttcgcact atctaatgtc ggcattgaac
    12061 caagcagtcc ccaagtatag ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa
    12121 cttcaacgat tcttcctcca tacatgacag cgacacgatc agcgacattc gctactacac
    12181 caaggtcatg agtgataaag ataattgatg tatcgatttt tgtctgaatt tctttcagca
    12241 gctccaaaat ctgtgcttga atagtcacat caagcgctgt tgtaggttca tcagcaatca
    12301 ggatttccgg ataacatacc agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg
    12361 agaactgatg cggataactg ttgatacgtt tctcagcttc gggtattcca actaatttaa
    12421 gcaattctaa tgcagctttt tgtccctctt tttttgaaac tttattatgc tttctaaggg
    12481 actcagcgac ttgcttccca atgggcatcg tcggatctaa agaggtcatt ggatcttgga
    12541 aaatcatcgc tatttcttta ccacggattt tttgcatctc tctttctgat tttccaacga
    12601 tatcgttacc tttaaagagg atttgaccag catctatatt ggcgttgctt gacagcaagc
    12661 gcatgatgct tctggtcgtc actgattttc cacttccaga ttccccaacg atcgccaatg
    12721 tttctccttt attcaaatga aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg
    12781 tatcaaaaga gatttctaag tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt
    12841 cattttagga tcaaaggcat cacgcaatcc atcagcaagt aagttaaagc caatcatgat
    12901 aaccgataaa gttactgccg gaatccataa tagataaggc agatacagga atgtcttgta
    12961 cccttcgctc aacatcgttc ctaatgaagc tgacggaggc gtaagaccta aaccgataaa
    13021 gcttaaaaag gcttcgaaga aaatagcaga tggaatacta aacatcattt gaataataat
    13081 gacactagag atattcggca aaatatgttt gaatgcaatt ttcattcttg attcacctaa
    13141 tgtcaatcca gctaaaacat attcttgatc ttttaatttc attgtttgtg cacgaacgat
    13201 acgtgccatt ggtatccagt ttgtgatggc catagcagcc acgattgaaa aaattcctgg
    13261 ttcaaaaacg accaacatca aaatcatgac aacgaggttt ggaattcctg ataagatttc
    13321 caaaaaacgt tgcatcacgt tatcgacacg tccgcctaac aaaccagaaa ccaacccgta
    13381 agctacacca atcgttacat ctagtaatgc agcaataaat gcgatcagca acgagattct
    13441 cattcccata aataagcggc ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa
    13501 atttacgttt gaagggacgt ttgcttgagc atatttatca acaagttcac caccaaccat
    13561 tgcttttcca ttcaatccat cgatattgat tcccggaata cgaggaggca aattgatata
    13621 agccacgttt tgggcagttg gatcatgagg agaaacaaag attgtgatca cagagatcaa
    13681 aatgatgacc aatagtaaaa ccattgaaat aacggcagct ttgtttttct ttaaacggcg
    13741 ccatgaatct tggataaaat tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt
    13801 atttttttgt aatggaagaa attcatccgc agggatatct gcaactgttt cataacgctt
    13861 tggtatcatt tccatttatc ctcggctccc ttctgaaaca cgaattcttg gatcaactag
    13921 accgtacaat agatccacga tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt
    13981 tacagccatg atcgtaggat aatcatttgt tgtgattgac ttaacgaatt gttccccgat
    14041 accaggaata gcaaagatat tttctaccac cagtgatccg gtcatcaatg ctacagctaa
    14101 cggtcctaat aaagtcatca atggaatcaa actatttcga agaccatgtt taaaggcgat
    14161 ttcccaacgg ctcaatcctt ttgcttttgc taattccaca taatcactat gcagtacttc
    14221 caccatttcc gtacggataa accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat
    14281 tgttggtaag attgtgtaca caaagccatc ccattttgcg attggcaaaa cttgtaattt
    14341 gattgcaaag atatattgta ataaaacagc aaatacaaag tttggaatgg aacgacctaa
    14401 tatcgctacg agtgtagaag atgtatctgc ccatgtattt tgcttcatgg cagagatcgt
    14461 tcctaaaatc gtccctagga ctgttccaaa gataattgcc tgtaacccta actgcaagga
    14521 tggtccgatt cttccagcta acaaatgtgc gactggctgg tttttaaatt ggaaggaaat
    14581 tccaaaatct ccttgaagca aattagaaag ataaatccca tattgtacga taactggttt
    14641 atccaaaccg acttgtttat ttagcatctc gattgtttca ggactcaaac gttcctgatt
    14701 cgtatatggc gtgcccggca gcaattgcat caagaaaaag gtgattgttg caattaacca
    14761 aagcgtgatg atcatgaaaa agacacgttt taatacatat ttgatataac tattcactaa
    14821 tgtttccccc tagcttttga cttctgacag atgtttcgct acattattag ataatgaacc
    14881 aaaaataaag gagcaatcaa atgaaaaaaa aatctatccc ctacgcagta gcttttctac
    14941 tgattctggt tattttaata aaaaatctca tcaaccattc ctttacactc attcaattat
    15001 ctaatgatct attcttatgg tcattgccgt ttctgattat cggcggtttt ttatgggtct
    15061 tttcttcggg tttcttcgat cattttcagc gatcggtcca tcttgcacga acaaggaacc
    15121 gaaagaaaaa accagaattt tccacgctct cgtctgccag ctacggtatg tacagctttt
    15181 ggctgatcat tgccggaata ctaattgctc tttctgctat tttcatgctt ttttctttgt
    15241 taggataagt gaatatgaga acgaggttgc gacatttgcc gcagcctcgt tttttctcat
    15301 gctcacggta ttaacgatta ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct
    15361 gccgcatggt gaacgatacc tttgattttt tcattcacta ggtgaccttc tgctttttgg
    15421 tacaccggaa cgacacccat atcatcagag atgatcttga ttgctttttg gaaatcatcc
    15481 caacgggctt gttcatcgcc agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat
    15541 tctgcattgc tccagcgacc acggttgtat gaattacctg tgacaaacaa atcagtaaag
    15601 ctgcttgggt ctgcatagtc agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa
    15661 gttgagcggt caagacgtac agagaatgga acaggtgttg gtttgactgt tacgccatcc
    15721 aagttttctt ggattgagtt ttgaacatat tcgattactt ttttcgatga atcatcatca
    15781 gaagccatca aatcaaattc taaagaatca acgcccaatt cttttttcgc tttttcccaa
    15841 tattcttttg ctttatcttt gtcataagaa actaattttc cagcttcttt tgcaaagtct
    15901 tcgttcgttg ttggactttt taccatatct gatgggatca atcctgtaga gacaactgat
    15961 ccgtcaccta gtacttgttt tactaaagct tcacgatcga tagaataaga aatagctttt
    16021 cttaggttca cattgttgaa tggtgaattc ttttcacgtt gattgaattc gatatagctt
    16081 gtccgcgctt ctttaacaga agtataagct ggatcgtttg cgttttgctg tgctaactca
    16141 ccagacaaga tcacgtcatc tgcttggcca tctttgaata agttcaatgc agtagaagat
    16201 tcttttacta cactgacttt gatttcagaa agttttactg tatctttgtc ccagtaatga
    16261 tcatttttct tataagtcca ttctgtatct gttcctggtc catcaaaatc ttctaatgtg
    16321 aatggtccat tataaacagc tttatcactt gcagatgcat aggcatcccc attttcttcc
    16381 actacggatt gtttttgtgg gaaaaatgat gggaaagcaa gcagatattg gaaatatggt
    16441 gtttgtttcg tcaatttgat ttctaattca tagtcaccaa ctgctttgat ccctagttct
    16501 gatttatctt tctttccagc tgtaatgtct tctgcatttt caactggcgc atacaggtaa
    16561 gcatattcag aagctgtagc tgggtctact gttcgttgcc agccataaac gtaatccgct
    16621 gctgtaacag gatcaccatt tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt
    16681 aatccgtctt cactgacttc tgccaactcg ctagctcctg ccggctgtgg tttgctgtct
    16741 tcatcaagac ggtaaattcc ttcataaacg ttattcaatg ctgtaaaact gattgtatct
    16801 gttgcaactg ataagtcagc tgtaggcatt tcttgaggca cgactaagtt aaatacgcct
    16861 cctccatctg ctgaaccgct ttttgctgaa ctactgcttg tggctgaact gccgccataa
    16921 catccagcaa gtacaaggcc gcataccgca accaaaccaa atgtaaattt ctttttcatt
//
LOCUS       DASJST010000061        16941 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJST010000061 DASJST010000000
VERSION     DASJST010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.061"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="U4B05_001552"
     CDS             <1..1152
                     /locus_tag="U4B05_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEN2050695.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="U4B05_001553"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="U4B05_001553"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN2050696.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="U4B05_001554"
     CDS             2728..3003
                     /locus_tag="U4B05_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN2050697.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="U4B05_001555"
     CDS             3009..3776
                     /locus_tag="U4B05_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN2050698.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="U4B05_001556"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="U4B05_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN2050699.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="U4B05_001557"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="U4B05_001557"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN2050700.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="U4B05_001558"
     CDS             7920..8222
                     /locus_tag="U4B05_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN2050701.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="U4B05_001559"
     CDS             8225..9388
                     /locus_tag="U4B05_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2050702.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="U4B05_001560"
     CDS             9414..12842
                     /locus_tag="U4B05_001560"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN2050703.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="U4B05_001561"
     CDS             12863..13987
                     /locus_tag="U4B05_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050704.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="U4B05_001562"
     CDS             13977..14414
                     /locus_tag="U4B05_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2050705.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="U4B05_001563"
     CDS             14398..14736
                     /locus_tag="U4B05_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN2050706.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="U4B05_001564"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="U4B05_001564"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN2050707.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="U4B05_001565"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="U4B05_001565"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN2050708.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="U4B05_001566"
     CDS             15893..16936
                     /locus_tag="U4B05_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN2050709.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5738 a         2932 c         3739 g         4532 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacattg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact a
//
LOCUS       DASJST010000062        15961 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJST010000062 DASJST010000000
VERSION     DASJST010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.062"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="U4B05_001567"
     CDS             226..894
                     /locus_tag="U4B05_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN2050710.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="U4B05_001568"
     CDS             complement(1094..1324)
                     /locus_tag="U4B05_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050711.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="U4B05_001569"
     CDS             complement(1451..2833)
                     /locus_tag="U4B05_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2050712.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="U4B05_001570"
     CDS             complement(2989..4176)
                     /locus_tag="U4B05_001570"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN2050713.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="U4B05_001571"
     CDS             complement(4183..5214)
                     /locus_tag="U4B05_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2050714.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="U4B05_001572"
     CDS             complement(5260..6684)
                     /locus_tag="U4B05_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2050715.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="U4B05_001573"
     CDS             6859..7764
                     /locus_tag="U4B05_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2050716.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="U4B05_001574"
     CDS             7855..8532
                     /locus_tag="U4B05_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2050717.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="U4B05_001575"
     CDS             8529..8699
                     /locus_tag="U4B05_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050718.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="U4B05_001576"
     CDS             8813..9709
                     /locus_tag="U4B05_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2050719.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="U4B05_001577"
     CDS             complement(9764..11134)
                     /locus_tag="U4B05_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2050720.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="U4B05_001578"
     CDS             11372..12118
                     /locus_tag="U4B05_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2050721.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="U4B05_001579"
     CDS             12115..12954
                     /locus_tag="U4B05_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2050722.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="U4B05_001580"
     CDS             12955..13905
                     /locus_tag="U4B05_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN2050723.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="U4B05_001581"
     CDS             14046..14798
                     /locus_tag="U4B05_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050724.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..>15961
                     /gene="queG"
                     /locus_tag="U4B05_001582"
     CDS             14817..>15961
                     /gene="queG"
                     /locus_tag="U4B05_001582"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN2050725.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRK"
BASE COUNT         5174 a         2994 c         3220 g         4573 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 t
//
LOCUS       DASJST010000063        15186 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJST010000063 DASJST010000000
VERSION     DASJST010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.063"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..409
                     /locus_tag="U4B05_001583"
     CDS             68..409
                     /locus_tag="U4B05_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050726.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(402..593)
                     /locus_tag="U4B05_001584"
     CDS             complement(402..593)
                     /locus_tag="U4B05_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEN2050727.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            943..1155
                     /locus_tag="U4B05_001585"
     CDS             943..1155
                     /locus_tag="U4B05_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050728.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            1162..1386
                     /locus_tag="U4B05_001586"
     CDS             1162..1386
                     /locus_tag="U4B05_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050729.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            1413..1946
                     /locus_tag="U4B05_001587"
     CDS             1413..1946
                     /locus_tag="U4B05_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN2050730.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            1933..3177
                     /locus_tag="U4B05_001588"
     CDS             1933..3177
                     /locus_tag="U4B05_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEN2050731.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            3190..4689
                     /locus_tag="U4B05_001589"
     CDS             3190..4689
                     /locus_tag="U4B05_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2050732.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            4694..5620
                     /locus_tag="U4B05_001590"
     CDS             4694..5620
                     /locus_tag="U4B05_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN2050733.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            5747..6349
                     /locus_tag="U4B05_001591"
     CDS             5747..6349
                     /locus_tag="U4B05_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN2050734.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            6362..7285
                     /locus_tag="U4B05_001592"
     CDS             6362..7285
                     /locus_tag="U4B05_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2050735.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            7303..7566
                     /locus_tag="U4B05_001593"
     CDS             7303..7566
                     /locus_tag="U4B05_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050736.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            7578..7907
                     /locus_tag="U4B05_001594"
     CDS             7578..7907
                     /locus_tag="U4B05_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEN2050737.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            7904..8230
                     /locus_tag="U4B05_001595"
     CDS             7904..8230
                     /locus_tag="U4B05_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050738.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            8214..8552
                     /locus_tag="U4B05_001596"
     CDS             8214..8552
                     /locus_tag="U4B05_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2050739.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            8552..8944
                     /locus_tag="U4B05_001597"
     CDS             8552..8944
                     /locus_tag="U4B05_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEN2050740.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            8955..9467
                     /locus_tag="U4B05_001598"
     CDS             8955..9467
                     /locus_tag="U4B05_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEN2050741.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            9513..9881
                     /locus_tag="U4B05_001599"
     CDS             9513..9881
                     /locus_tag="U4B05_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HEN2050742.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            9935..10222
                     /locus_tag="U4B05_001600"
     CDS             9935..10222
                     /locus_tag="U4B05_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050743.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            10239..13358
                     /locus_tag="U4B05_001601"
     CDS             10239..13358
                     /locus_tag="U4B05_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2050744.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            13391..14158
                     /locus_tag="U4B05_001602"
     CDS             13391..14158
                     /locus_tag="U4B05_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEN2050745.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            14155..>15186
                     /locus_tag="U4B05_001603"
     CDS             14155..>15186
                     /locus_tag="U4B05_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2050746.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNR"
BASE COUNT         5438 a         2294 c         3237 g         4217 t
ORIGIN      
        1 ttctaaattg ttcttattat ctatcatccg ttgctgttta ttaatttatg tattggaggg
       61 aaaacgaatg gaagtcgagc aaataaagta tcaaggtatg gatcgggaac ggttgattga
      121 tattattgag caacaacgtt tagaattgat ccaaaaagaa gctgtatgta aagagtacaa
      181 aaaacattta gaacaagtaa ttgagtatca ttcagtagaa aagtacagat cagtggtgca
      241 aaaaaataga gcaacgagtc ctgatcctcg tcaagatgta tcgattagta tcaagacgcc
      301 agtatttaat tctgttaaat cagaaaagct agaaagtatc tcaactgctt tggtagaaaa
      361 agcagctgaa atgaactccc tagcaaatga agttagtcgt cttatttagt cacatcgata
      421 acttgagagt ctttggtgat tccgtttttt atagaattaa tggtttgttc agcggaatat
      481 ttagtcaagt atgtttcgct agttgctact acttcattgt tatcggactt aataacgaag
      541 taatattggc cattagttgc ttctcttata acaaagtaca aatttttcac caccttttaa
      601 tttatttcag cggaccactc gctgataaat aaaattatac acttagtatt tattttcaca
      661 atattaattt gtcactgtgg cggaaagggt agacgctaag catgtgtgtt aggtcaatgc
      721 ttcggcaacc atgcaaggtt cgattccttg ccagtgactt tggcaactga gggttggaaa
      781 tgggcgctca aagtacacga gcaaggcgag gtcgatagta atcgatggaa tcggtgtagg
      841 ttgcttgata gaactataac tgcatctcgt ttatgaggtg tagtttttac atattagatc
      901 actcatcgag tggtcttttt attttggaag gggagtaaaa aaatgaacga aaaccaatta
      961 agagagttgt ttaaaacgaa tgaagcaaac aaaacaatgg aggcgacatt ctacgaaact
     1021 caaaaaagct tagcgttaat cgcaaaacaa gctaagtatt tctacgatca gcttattctg
     1081 caaggattta atgacgcaca ggctatggaa tttatgatgc gaaccttttc tgccagtaac
     1141 caacagaaag agtgatacgt aatgaggaac tactggtatg tatcattatc aaataaatat
     1201 ccgcaaccaa acgcagatga tccaattaga gttgtccagt cagtccagat taaaaagaag
     1261 tactccatca ttgaaatgac cagagaagct acgccaaaag agattgataa gtacaatctt
     1321 cgttactgcg gccatggata ttttagtgag cagaacatac agacaaatat tgagagatat
     1381 tgttaataaa attgaaaggt ggtggcttga atgtggtaaa tttgacacca aaacaaaaag
     1441 cttttgcgga tgaatatata aaaaatggtg gtaatgctac tcaagccgcc attaaagcag
     1501 gttatagcaa acgatcagca agagtaatag gtaaggaaaa cctaactaaa cctaacataa
     1561 tacagtatat taatgaacgg ctaaatccta tcgaaaagaa gcgcaaatta agtgccgagg
     1621 acgctttaaa tgaattgata gatatttggc aaggagaagt acaaataagc gtgagcaagc
     1681 aaatagaccg cttggagaaa aacaaggtta ttaaacatat gcaatatgaa tatacaccag
     1741 atttagaaag caaattgaaa gccttggatt tgtatttgaa gtataaatcg ctgttatcac
     1801 aaacgcaatt agaaaaagct caaacagaaa taaaattaat gcaagcaaaa ttagaacaat
     1861 tacagataaa ctcagagcgt tctaccgaag aaaaacttga tgagttgtta gaaaagatta
     1921 gtggtgaatt agatggcact agttgatatt tataacccaa agcaaatcga cgtgttaaat
     1981 aaaaccatta agaatgattg gtttattact ttattacatg gagcaaaacg ttctggaaaa
     2041 acaaaaataa acaatgattt gttcttgttt gaattgcgac gtgtgcgaaa gattgccgat
     2101 gaagaagaca ttaaggaacc aatgtatatc ctagcaggag tttcaagtgc aacaatccaa
     2161 aagaacatct tacaggaact atacaacatg tacagcatag aacctaaatt cgataaacat
     2221 ggaaacttta agctatttgg cgttaaggtc gtacaagctt atacaggaaa cattggcggt
     2281 gttggcgcaa ttcgtggtat gacagcatat ggcgcttata tcaatgaggc atcgctagct
     2341 agacaagaag tatttgctga aatcgtttca cgttgttcag gaacaggagc gagaatacta
     2401 gctgatacaa accctgacaa cccggaacac tggctaaaga aagagtatat cgacaattca
     2461 agcaaaaata ttcaatcgtt ccactttgaa ttagatgata atacattttt atctgaacga
     2521 tatcgtaaca atattaaaga atcaacacca agcggcatgt tttatgatcg tgatatcaaa
     2581 ggtttatggg tttctgcaga aggtgtcgtt tatcgtgatt ttgatgctag caagcactac
     2641 atccagtcaa aagacttacc acctttgagc aacttttatt gtggtgttga ctggggatat
     2701 gaacactggg gttcaatcgt agttataggt gaaacggatg acggaacagc ttatttaatc
     2761 gaagaacatg ctactcaatt tgaagagatt gattattggg tagatgtggc aaaaggcatt
     2821 caagagcgtt acggcttacg agtacctttt tattgtgact ctgcgagacc ggagcatgtg
     2881 gctaggtttg taagagaaaa gattaatgct aaaaatgctc ataaagcacg gttatctgga
     2941 gttgaggaag tcgccaagag atttaaacaa gataaattgt ttatctgtca agatagagtg
     3001 atgaaatttc gggatgaaat ttatcaatac atttgggaca agaaaaaagg cgagccaata
     3061 aaagaatatg atgatgtgct agactccgtt cgatatgcga tatatactca tgagcttctt
     3121 aagaaaccaa aagttaatgt caacgaaaag attaaacgta ttaaacgcat gttttaagga
     3181 gtgtgaaaaa tggataaggt aaacgagttt gaatacggag ctgatataca ttattctaat
     3241 gacgtgaaca caaattatgt aaagtttagc gtagactcaa atcttcacta tagatttagc
     3301 tcagcagaag atttacttaa cgatttagat actttagcag caatgataaa acatcatcat
     3361 gaatatcagg taaaaaggct aagtgtatta gatgattatt ataaagctag aaatacaaat
     3421 atcatggata accgtaggcg cagagaaaaa gaaaaagcgg atcaccgatc agcacataac
     3481 tttggaaaag ttctttgtac gtttgatgtt gggtacaaca caggcaatcc tataaaagtg
     3541 caaatcgagg acacaaatca acaaaaagaa atcgaagagt ttaatactaa taatgacata
     3601 gatgggttaa atgctgaact ctggcttgat atggataagt atgggagagc ctatgagatt
     3661 atctatcgag attcagatga tacagattat gttgatttgg ctaatgtatt tgaaacgttt
     3721 gttgtatatg atactacagt aaaacgagag cctattttgg ctgtacggta tcctaagaca
     3781 agattcaaca aggatgctga taaacagtac attcaaccaa tcgtatacac aaaagaaaaa
     3841 agtatcactt atgatgagac gacactaaca gcaattgagt taaagaatcc ccaggatgaa
     3901 ccgcatgaat ataaagaggt acctattaca gagtattctc ctaatcgttt tcggatgggc
     3961 ttgtatgaag atgtactatc tttgattgat ctatacgatg cagggcagtc tgataccgcc
     4021 aattatatga ctgatctaaa cgatgctctt ctagttatta gtggtgatat tgaagcagca
     4081 ggactatcca cagaggacgc catcaagcag aaagaagcga atatgctttt gcttgagtct
     4141 ggaactgatg tgaacggtaa taaaacaagt gtgactgcag gatatattta caaacaatat
     4201 gatgtgaacg gtgtagaagc atacaaatac agagtacgca aggatattca cgaaatatcc
     4261 atggttcctg atcttactga tgacaatttt tccggagtgc aatcgggaga agcaatgaaa
     4321 tataaattat ttggatttga acaaatgacg gcaacaaagc aaaggctatt caaaaaaggc
     4381 cttatgcggc gttatcgtct tttatttagc ctaaaatcaa gtatttctga aatggataac
     4441 tccgatttga aaggcttacg tgtaatattt acgcctaatc tacctaaagc cattctggaa
     4501 gagttgaaat ctttggttga tgctggagct gaactcagtc aagagacgat cttaggactc
     4561 gcttcttttg ttccagatgt acaggcagag ttgaaacgag taaataaaga aacgcaaaag
     4621 cagattggca tttttgattc agatggtgaa gaagtaatta acaacaaaaa agatgaaaca
     4681 ggggggtgat taaatgaact cccaagaata ttggatcaaa cgggaaaagg aatggcaaaa
     4741 gcaacaaatt aaagatgata aaaagcgcat ggcagaaatt aaaagtcgca tgcaatacgc
     4801 acaagatgcg atacaaaaag aaatagacgc gcagtgggac agtttctcca atggccagaa
     4861 aatcactcgt agcgaagcga tgaagcgtgc tagtgaaatg gatgtcaaag cattcgctcg
     4921 caaagcaaag aagtatgtca aagagaaaga tttttctcct acagcaaacc aagaattaaa
     4981 gctatacaat cttacgatgc gtgtaaatag attagagctc ttaaaagcta atatcgggct
     5041 tgaattgatt tcactgttta atgaattgga taagtacttt tcgaatgaat taacaaaagc
     5101 tggtttagct gaattgaaga gacaagccgg tattttagaa atgactattg cttcaagtgg
     5161 atatgcaaag ctgatagaac tagtaataaa cagctccttt ttgagtgatg acgtgtcttt
     5221 tagtgatcgc ttatggatgt atcaatctga attgaaatca gaattagata ggttgttagt
     5281 cagaagtata acgatgggga aaaatcccaa gcaacttgca tctaaattgg cagaatattt
     5341 aacagctgaa ggacgagaaa acactaagtt caacactcaa cgtttgatgg tgactgaaac
     5401 gactagagtt caggtaggga ttcaagaacg aagttataga gatgcaggca ttacccagta
     5461 catctatata gcagaaccaa cggcgtgcaa actatgtata ccgttaaata atcaagtttt
     5521 tgatgttgcc gatatgcagc caggaagtaa cgctcctaat atgcatccat tttgtcgatg
     5581 cagtacagca ccttatatag aacgaatatc aagccgttaa cacaaattaa cggcttttta
     5641 ttgtgccttc ttacagctta caggcgttaa agagaaagct attttcggct gaccggcgta
     5701 actggtcaaa tttatcgggt agcggcgtaa ccgtggagga ttaatcatga aaaaacgttt
     5761 atttatgcca atgaacttac aatttttttc tgaaccagga gatggtggat ctggtgatga
     5821 gggacaacaa ggaaacctac cagctggctc acaagagaca ccgcccgaag caaaagaaga
     5881 aaacaatact ggcaaaacat tttctcgtga tgaagtagcg aaaatgatcg ctgctgagac
     5941 gaataaagca aaagcagcgt gggaaaaaga actagcagca aaaaaagaag aagctaaaaa
     6001 gctggcaaaa atgaatgcgg aagaaaaact acagcatgag ttggagcaaa aagaagctga
     6061 aatcgctgaa ttaaagcgtg gacaggcact atctgaaatg acgaaagaag cttctaaaat
     6121 gctgacagat gcaaatttac cacacgatga tgatttactt ggtctgattg tttctgatga
     6181 tgcagatgcc acaaaacaag ctgtagcagt catcactaac tttgcttctt tgattaagag
     6241 agaaaacgca agacaaacac caccaaatga aggtggacaa tttacagcat cgaaaaatac
     6301 taaagaaaca gtggctaaac tagctgctaa aaatcgaatt atcaaatagg aggaaaactt
     6361 aatgaaaaag aaacaacttt taccaatgaa cttgcaaatg tttgctcaaa catgggatcc
     6421 agataatgtc ttggtatatg aaacgaaaga gggaaaaatt cctgataaat ataatacgct
     6481 cattttgagt gaagttatgg aaaattctaa gatcatgcag ttagcaaaat acgaagaaat
     6541 gactgacaaa gaaaagaaat ttgaatactt tgcagaagga ccaggcgcat actgggtggg
     6601 tgaaggtgaa aaaattaaaa cgtctaaacc taaatggatg caagctacga tgactgcaaa
     6661 aaaactcggt gtcattcttc cggtttctcg tgaatattta aattataaat tatcagattt
     6721 ctttgaggag atgcagccaa aaattgctga agctttctat aaaaaatttg atgcagctgc
     6781 cttattaaat aaagaaaacc catttcctca gtcactagac ggatcagtta ttagtgcggg
     6841 gaatgtggtt gaaggcggat tgacttatga taatatccta gccttagaag acaagttagc
     6901 agaaaatgaa ttcgaaccta atgcgtttat ttcaaaccga aaaaatcgta cagaattacg
     6961 ttctgcagct caaacagtcg ggtcaaatgt tgagtttatt tatgatcgct ctactaatac
     7021 aattgacgga ttaccagtag tagaccttaa gtctttagat aaaggaacac tttacgctgg
     7081 agactttaat tacatgtttt atgggatccc atataatatt tcatttaaga tttctgaaga
     7141 agcccaattg tctactttaa ctaatgaaga tggaacccca gttaacttgt ttgagcaaga
     7201 actgattgct ttgcgtgcaa caatggatgt tggatttatg attgtaaaag atgaagcatt
     7261 tgggaagatt tccccaaaag cgtaacgcct gctaccggta ttgtgccaaa tcaaaagaca
     7321 tggaccggta aagtaggcga tactaaaaca tttactattt cagctgtgcc tgcagatgct
     7381 agcgatgcag ctgctgttgt tgcagctact acagcaactt caagtgatgg agctatcgca
     7441 acagtgacca aaaatgaaaa tggtggtttt gatggaacga ttgcagcaga agggtcagca
     7501 acattcacat ttacttctgg agaattcact acttcaatca atgtgacagg tcaacctgct
     7561 agttaggaag taaaaatatg atgattgcag atgatattaa aaaacttctt aaaggaacaa
     7621 tagatgaaaa gcttgaagtt attgagcgaa gaacgaatga gcgtatgaaa accttgttaa
     7681 atacgcaaga agttcctaaa gaatttgaaa cagttgtata tgaagtatcg ttgaaaagat
     7741 tcaatagaat tggtcaagaa ggtatgcagt catattctca agaaggttta tctatggctt
     7801 ttcctgactc ggatttttca gagtatcaaa atgagattga tgaatttaag cgtaaagatc
     7861 aggaagagtt gtacaagcca aagcgaggga ggtttaaatt tgtatgagat ttacagatga
     7921 aattatattt gttaaacgtt catctgactc taaatatgat ccagatctcg gtgagtgggt
     7981 tgaaggcaaa ccagaaagaa caagaacaga ggcaaacgtg acagatattg gcactgatag
     8041 aagtgtgact atttttggta gtgtggaaga aggggcgaag gtcattagga cgcagcctct
     8101 tttttctatc cctacatttg actatatcga gattgaagga aagacttggc aacaaaaaac
     8161 agctagaaat ccagcatata gaaatagttt aattgtgcaa gaggtggttc ttgatgaagg
     8221 cacaacttga atataaagga atcgatcagc tgatgcgaca tctgaaaaaa gcagcaacgc
     8281 ttaatgacgt tcaaaaagtc gtgaaaagta atactgctga aatgactgaa cgaatgcaaa
     8341 aaggtgcgcc agtggataca ggttacttac gaagatcaat aaacatgaat cttttagaag
     8401 ctggtttaac tggtattgta ggaccgacag cagattatgc accttatgta gaatatggca
     8461 ctcgatttat gtcggcacag ccttatgtta gaccagcgtt taattaccaa aaagtcaaat
     8521 ttatggctga aatgaaagcc ttggtgaaat gatgattaag acaagagatc aatcaatttt
     8581 tgatgaactt tttaaaatat cccaaaacaa acttggatat aaaacatacg attacaaaac
     8641 tttagaggat gttggttatc cttttgtgga atttgagaac actcagacaa tccatgaagt
     8701 aaataaaact gacattaaag ggtctgtgat tgtggtttta tccgtttggg gattacagaa
     8761 gaaacgaaag cagttgtcag atatggcatc tgctcttttt aatgaagcta gattgataga
     8821 agccacagaa ggctattatt gggctttaaa ttatcaagca agtggaattc aagtgatgga
     8881 cgacacaaca acgaatacac cgctaaaacg ggcggttgtc acacttgaat ttagaattag
     8941 ataggaggaa gaacatggaa gcattaaaag gtattgatgt cattttgctt tatcgcttat
     9001 tgaaaaaaga aactcaggaa gccgcttgga aaatggcatt tcaaacagaa cacgaaaatg
     9061 gtttatcaag agattcagac tctacagtga caaaagatgg aaacgttcaa agtttaagcc
     9121 cggttgaata tgatttttcg gctacttcaa tagttgctaa aggagattct catgtagatg
     9181 aaatgaaaca agctttatta aatggggata tcattgaaat ttgggaaatt aataaagcag
     9241 aacagggaac agatgataat gcaaataagt acaaagctac ttattaccaa gcatatgtgt
     9301 ctgaatttac tccatcggct gctgcagagg ataacgttga attaagttta tcatttgcag
     9361 taaatggtgt tggtcaagat ggttatgcaa ccttgacaga agatcaagcc gatgttgttc
     9421 aatatgcatt caaagatacc gtgaaagcaa cttcgacagg agcataagag ggcttagatg
     9481 ctctcttttt tattttagga ggatgaaaaa cattgaaatt aaaaattaaa ggtaaagaat
     9541 attcgtttaa atttggcact aaatttgtac gtgaattaga taaagtgatg cctttcgtcg
     9601 atggaaatat ggaatttgga atgggactct ccgcaaaagt cttaccagag ctacgttctt
     9661 ataacgttaa cacgttatca cgagttctgg aaatagcaaa tagaactgaa gatgaatcta
     9721 ttacattaga tgaattggat gattacatcg atgaagttaa agacattgaa aaattgtttg
     9781 atgaagtcct aaaagaattg gcggagtcga acgcgggaaa gttagcggtc cgaaacctga
     9841 atcagaaatt gaaagaagcg gaaaaacaac aagtggaata gattctgcac tggcatacga
     9901 acaaattctt atcaattctt ttcgatattt gggaatgacc aatatctcag atatcgaaag
     9961 aatgacgtta tatgaataca acattcgtat gactgcagcc cagttatctt ggcttgacaa
    10021 agaaaagttg attcacgaat tagcgtgggc aaatcagcaa gtccaagcgg agaaaaaagt
    10081 aggcaaaaag acagttcctg tatatcgatc ctttgaagaa ttcttcaatt atcaaaaaat
    10141 cgaagattca atcatgggag tttccgaact ttcaaaacaa gataaaaaat tccaaagctt
    10201 actaactaaa gctaactctt gaggaaagga ggagaatcat ggaacaattt tctgttgaag
    10261 ccttattaaa agccacagat agtggatttg taaagacttt taaagatgca caagatgctg
    10321 ttaaaacttt tgaaaagaat tcaaatagta tgacaaccgc tgttggtaaa gtgatgcaag
    10381 gtactggtgc cgcaatgaca aagtatatta ccactcctct tataggagta ggcgtagcag
    10441 ctgctaaagt tggtggcgac ttcgaagcac aaatgagtcg tgtaaaagct atatcaggag
    10501 caaccggcga cacattcgaa cagatgaaac agcaagcgat tgatctagga gcaaaaactg
    10561 cttttagcgc aaaagaatca gctgctggaa tggaaaactt agcttctgct ggatttagcg
    10621 cacaagaaat catgaaagca atgccgggtc ttttagactt agcagctgta tctggagggg
    10681 atgtggctct agcttctgaa aatactgcta ctgctttgag aggatttggt ttagaagcaa
    10741 gtgaagcagg acatgtcgct gatgtatttg ctcgtgctgc tgcggatacc aatgctgaag
    10801 ttggagacat gggagaagca ttgaagtatg ttgctcctgt agctaattca atggggattt
    10861 ctttggaaga aactgcagca gctattggta ttatgagtga cgcaggcatt aagggttctc
    10921 aagcaggtac aacgttgcga ggagcattgt ctaggttagc aaggccaaca aaggctatgc
    10981 aagatacaat ggataattta ggtgtttcgt tttatgatgc tgacggtaaa atgaaacctt
    11041 taaaaactca agtagaatta cttaaaaaag cttttgaagg cctgacgcct gaacaacaac
    11101 aaaatgcttt agtaacacta tatgggcaag aatcattatc agggatgatg gctttgattg
    11161 ataaaggacc tgattcattg ggcaaattaa caaaatctct gaaagattct gatggtgcag
    11221 ctgacgatat ggctcggacc atgcaagata atatgaattc ttccatcgag caaatgtttg
    11281 gagcttttga gtcagcagct attgtaattc aaaagattct agcaccatcc atcaaaaaag
    11341 tagcagatgc catatccggc ttagtagaaa aatttgtaag tgctccagaa tcaactcaaa
    11401 aattagtagt ggccatagga gcaattgtcg ctgctatagg accgttaatt tttatgattg
    11461 gttcagtaat tatatggatc aatagggtga aagtagcttt taaagcttta agtgaaagtt
    11521 caaaattgtt tagtggatta agtaaagcaa tgggtcttct tacaaatccg gtttttctgg
    11581 ttatagctgc ggtagcacta ctcgttgtag gtttcatcta tctttggaat acgagtgaag
    11641 attttagaaa cttttggatt ggcttatggg agggaatcaa gtctgctgta agctcggcag
    11701 tagaatggat tcagaatgca tggaaatcta caggagaatg gtttaacaat ttatggaagt
    11761 ccattaaaga aggcgcagac aatgtttgga ctacagttca agaagcccct gggaaagcgg
    11821 cagattggat taaaaacaaa tggactggaa caaaagagtt cttttcaaat ttatggtcaa
    11881 gtattgcaaa ctctgcttca gagatttgga atagtttaaa agaaggtgtc atatcagtta
    11941 ttgatgattt agtttcaagt gctggtgaaa aatgggaagg gtttaaaaat actatatcta
    12001 ctgcatggaa aacaattaca agtaaaatca aatctggttt tgattttata ctaaaatata
    12061 ttggtccatt tgtaagtagc ttttcagatg tgttctctaa tatagtgaaa gcaataacaa
    12121 gtatatttgc tgaggttaaa aacataatag taaatgcttg ggaaatcatt aagtctttaa
    12181 tagctgcgcc actactgttt attatagatt taattactgg tgacttcgaa caaatgaaag
    12241 aggatttaga tctaatctgg aacacacttg tccaatcagt ggtaaatatt tggacatctg
    12301 taaaaaatat atttacggaa tatatcggtg caatagtaaa tagtgccgtt agtttatgga
    12361 ctggattcat acaaagtatt tctaatattt ggaatgaagt agtttatcaa gcgactatga
    12421 tttggattga tttgaaacta ttttttacta atttatggat tgatattaaa tacagtgcaa
    12481 ttcaaatgtg gataaatcta aaattctcca taattcaaac ttggattgat acaaaatatg
    12541 gtgcaattga actttggaat aatctaaaac aatggttttt ccaaacggtt aataatatcg
    12601 tgcaaactct tataaaaagt tggaacagct taaagcaagg aacgatagat ttattcaaca
    12661 atacagttca aggtgctaaa gacatttgga cttcattcaa atcttggatt ggtgatttaa
    12721 ttactggaac caaagataac atcattcagg gatgggaaaa cctaaaacaa ggtactatag
    12781 atactttcaa caatttagta aatggtgctc aagaggcatg ggataattta gtaaatgctg
    12841 ttagtgacac ggttgataga gtaactggct ggtttgataa cttgaaaaat atcgatttac
    12901 tagcagccgg aaaagctatc atggatagtt ttctagaagg gttacaaaat gcatggaaat
    12961 ctgtgcaaga ttttgttgga ggtattggtg attggattcg tgaacacaaa ggacctatcc
    13021 aatacgatag aaagctattg attccagctg gtcaggctat tatgaacggt ctgaataaag
    13081 gtctgacagg aggattcaat gacgtacaga atactgttgg aagtatggca gactttatcg
    13141 cggaactttt caatgcaaat tctgatgtag atatagctgc aaatttgaaa aatgcaaata
    13201 aaaacattgg tgcacaagtt gaacataaaa taaatatggg tggctctact aaaccagctg
    13261 tatttaaaat caatcttgga agacaatcgt ttagattgtt tatggacgat atttcacaag
    13321 ctatgggcga aggtgcagat attaatctgg aattttagga gggaatattt tggatcaacg
    13381 agaaaataaa atgtactcat tcaaagatac aaccattaat ctcactagtt ctaaacgatt
    13441 ccttccgacg tctgccatga tgtacgatgg aatgtattta gaagatttga ttgatggtta
    13501 tcaaacactt acggtggaag gtagagaaat gctttctgta gaagttgaac agcaagagat
    13561 acaaattggt tcaatcatta caaatcagaa aataccttca agaacactaa aaataacata
    13621 taagctggaa gatagagatc cagaaaaact acagtttaaa ttcaaagaac tgttgaatta
    13681 tttataccgg aatgaagacg tggaaattag gtttcatgat gaattagatt attattacta
    13741 cggtcgctat acatcaactg atactgttcc aggagactcc aactcgatta tttcgagttt
    13801 taatgtattc tgtgcggatc cactaaagta tacgaaagag tgtgttagtg atggctatat
    13861 tggaaatccg atacagtttc ctataacacc aagaaaaatt gaagttactt tatccatgaa
    13921 taattcaatc aaaattacaa acggagaaca aaatatcacg ataactgacg cggcaataaa
    13981 aacaggagac gtgttggttt ttgatttttc cgatgagcag gtaactgtaa acggagaaga
    14041 ttgtacttct atgattgatt tagaaagtga ttttgagaac ttttatctta agcaaggtca
    14101 gaagataact agcaataatg ggaagcttaa aatattctat aggggggcga caatttgagt
    14161 gagacagttt atttctttga tcacttgcaa aaacttatta aaagaaaaaa tacaagaagt
    14221 ttgattgaag tctcccaaga aaaagaaatt agttctgata agagtgatct aatgaaagat
    14281 actctttacg ttacgacaaa atatgataaa gaaatagagg atgcaagata tatggcgatt
    14341 cgtgaaaacg agtcgtcttt ttcgttgtat cgaattacta aagttagcga cccatttgaa
    14401 acattagagt ttacagggtt aggatttgcg acaaatgaat tagatgctta catcatcaaa
    14461 gatattaggc cgagtgggca gcccttaaaa aatgttcttg atcgattgat tgaatttact
    14521 gaaggaaatt ggcgcgttgg tcacgtagaa gcaatgttac caacagtaac tgcaactttt
    14581 tactatgtct ctgtaaaaga agcgttgaaa gaattgcaaa ccttaggtat ggaatttgtc
    14641 tttaggtgtt ctttgaattc tgatggaata aaggataaat ggatcgaagt atatgaacaa
    14701 attggcgaag aatcgaatac acgtttcgta tatggtagta aagcattaac agttgtaaga
    14761 gagatagata gaagctcaat ctcaacttca atgataggtc gtgggcgagg cgaagaggtt
    14821 ggtgacggtt acggtagaag aattgaattc actgatgttg aatggaaaaa gtcgaatggt
    14881 gatcctttag ataagcctaa aggccaaaat tggcttgaag atccggaatc aactcaaaag
    14941 tatgggatac cacaaaaaga tggatcaatg agaaaacgag aaaccgtagt agtgtttgat
    15001 gatatagatg atccaacaga tttacttaaa aatacttatt caaccttaat cgattctgct
    15061 agaccgttag tacaattcaa agctgaagtc actggaggag atgtgatagg aaatacagtg
    15121 actattcaca gatacgataa aggttatcac tataaaactc gtatttataa aactacattc
    15181 aatcgg
//
LOCUS       DASJST010000064        14776 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJST010000064 DASJST010000000
VERSION     DASJST010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.064"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..1772
                     /gene="yfcC"
                     /locus_tag="U4B05_001604"
     CDS             33..1772
                     /gene="yfcC"
                     /locus_tag="U4B05_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN2050747.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1929..3113
                     /gene="iadA"
                     /locus_tag="U4B05_001605"
     CDS             1929..3113
                     /gene="iadA"
                     /locus_tag="U4B05_001605"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN2050748.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3224..3427
                     /locus_tag="U4B05_001606"
     CDS             3224..3427
                     /locus_tag="U4B05_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2050749.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3528..4190)
                     /locus_tag="U4B05_001607"
     CDS             complement(3528..4190)
                     /locus_tag="U4B05_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050750.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4203..5705)
                     /locus_tag="U4B05_001608"
     CDS             complement(4203..5705)
                     /locus_tag="U4B05_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2050751.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6253..7932
                     /locus_tag="U4B05_001609"
     CDS             6253..7932
                     /locus_tag="U4B05_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2050752.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8059..8538
                     /locus_tag="U4B05_001610"
     CDS             8059..8538
                     /locus_tag="U4B05_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN2050753.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8757..9437
                     /locus_tag="U4B05_001611"
     CDS             8757..9437
                     /locus_tag="U4B05_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN2050754.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9445..10776
                     /locus_tag="U4B05_001612"
     CDS             9445..10776
                     /locus_tag="U4B05_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2050755.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10791..11753
                     /locus_tag="U4B05_001613"
     CDS             10791..11753
                     /locus_tag="U4B05_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN2050756.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11864..12202
                     /locus_tag="U4B05_001614"
     CDS             11864..12202
                     /locus_tag="U4B05_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN2050757.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12281..12988
                     /locus_tag="U4B05_001615"
     CDS             12281..12988
                     /locus_tag="U4B05_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN2050758.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13091..14572)
                     /locus_tag="U4B05_001616"
     CDS             complement(13091..14572)
                     /locus_tag="U4B05_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050759.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4680 a         2544 c         3174 g         4378 t
ORIGIN      
        1 aaattactga aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac
       61 cgaaacgcga cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag
      121 tcatctttgc atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata
      181 ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct
      241 tccgttatgc ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag
      301 atcatccagc agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg
      361 ctgacggaga aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg
      421 tgctttacga tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag
      481 ccaaaatatg gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag
      541 gactagtatc tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg
      601 ttggaggtgc ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt
      661 ttatcagtaa aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt
      721 cattcggtgg cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg
      781 ttccatttgt cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg
      841 catctcaagt cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag
      901 ggattgctgg aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg
      961 ttactgcatt agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag
     1021 aaagttcgtt gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg
     1081 atgaaaataa aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc
     1141 ttgtctggat cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc
     1201 aattctttgt gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa
     1261 tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag
     1321 caatcgtagt aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg
     1381 ttccttcagc tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag
     1441 ctgtcattgg tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat
     1501 caaattcagg acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag
     1561 gtgtatccag acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt
     1621 ttacacccgt atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa
     1681 aatgggcaag attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca
     1741 tcatgattgc cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa
     1801 tccaaacagt agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa
     1861 gatcctatcg tttggattat tttttaagat cctatcatca gttatactta aagaaagagg
     1921 aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa
     1981 tggatgtact gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct
     2041 acgaaggagt ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca
     2101 ttgactgtca tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc
     2161 cagaagtcac attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg
     2221 gaacagacgg agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag
     2281 cagaaggaat ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa
     2341 cagattctat catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg
     2401 cagtatccga tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag
     2461 atgctcgtgt aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg
     2521 gaggaaaaag aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca
     2581 cacaattttt gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat
     2641 ttgccaaacg tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga
     2701 agacagatgg tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt
     2761 ctttagataa tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa
     2821 acaatcattt cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag
     2881 aagctgtgca aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc
     2941 cagcacgtat attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc
     3001 tgtgtatctt ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa
     3061 tggtccaaga aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag
     3121 ctctaactat cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta
     3181 ccctgaaggt agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg
     3241 acgttctaaa gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc
     3301 aacagacgac aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa
     3361 agaaaaagct cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa
     3421 aaaataaaaa ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg
     3481 cgagacagat atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt
     3541 ttttgaaatt ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc
     3601 tcatgagtca cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg
     3661 acgatatctt gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt
     3721 tgatgttcac agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt
     3781 ttggtcacct gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa
     3841 gcaacttgtc gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg
     3901 tctagagccg ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt
     3961 ttacgataac ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct
     4021 tcttttggtg catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt
     4081 gctcctaaaa tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc
     4141 gcatcttgcg gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc
     4201 ctttagttta ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag
     4261 acagaaaaca gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg
     4321 acattgattt ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct
     4381 tgttgaccat cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca
     4441 ggagcttgct ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg
     4501 gcattcgcga cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata
     4561 gccacttcgg taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag
     4621 acgcctaact catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata
     4681 ataccggcta cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa
     4741 ggttccaaca ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg
     4801 atcagcttct cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa
     4861 atagcagaat ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa
     4921 gaagtgtaaa gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt
     4981 gtttcgctgg attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca
     5041 gtactcgtga ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa
     5101 tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt
     5161 tttgctgttc catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga
     5221 aagtctgctt gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca
     5281 tcagtagttt ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct
     5341 tttgccatag ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct
     5401 attttttccg cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt
     5461 tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga
     5521 ttggcactta aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct
     5581 gcagcgcttc gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca
     5641 aacaataaca atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa
     5701 ttcatatttt gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct
     5761 agcttaaatc ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc
     5821 actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga
     5881 agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca
     5941 tagttgttct aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg
     6001 aacagctgaa tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt
     6061 taatgaagta tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta
     6121 attttcctgc tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa
     6181 tggctcatat atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa
     6241 gaggtgaagt ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga
     6301 aaaaacatgt atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa
     6361 tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa
     6421 gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg
     6481 gcacttcctt acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc
     6541 gagttagcaa aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat
     6601 gtggtagatg agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc
     6661 actcatacga taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt
     6721 tatacgggag attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca
     6781 cgattggcgg aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca
     6841 gaaaatcctc agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc
     6901 aaatattggg aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa
     6961 caagtattgg atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc
     7021 gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg
     7081 cttgttacgt tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact
     7141 ggccgaatgg gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat
     7201 gtcaagattg aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa
     7261 accatcgtgg cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc
     7321 tctgagaata tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat
     7381 ttgatgaaac caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat
     7441 gcagatttgg ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga
     7501 gacgtgctgg aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat
     7561 gtaatgattg atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa
     7621 atcttgtcag aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa
     7681 atcatttcaa aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat
     7741 ctgatgaaag aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat
     7801 gattttgagt ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt
     7861 gaacaaacaa aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga
     7921 aataaaaaat aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt
     7981 tttttgcgaa aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag
     8041 aataaaggga gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa
     8101 aagatcagct atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa
     8161 ctacaaaaaa gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac
     8221 aaaactgggg tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg
     8281 agacaagtga cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat
     8341 acaggatatc cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat
     8401 tgttttttag cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa
     8461 atcgggagaa gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac
     8521 gctgatttaa ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt
     8581 gagattatat ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac
     8641 atactgtact atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc
     8701 aagtgatgaa atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga
     8761 aaattacgta tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga
     8821 tgattgaccc gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga
     8881 cagattggtt gctgattact catggtcata gtgaccacat aggagatatg attcctattg
     8941 caaaagaaaa tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca
     9001 gaggagtaga ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg
     9061 taaaattcgt acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata
     9121 tgggcgagcc tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg
     9181 atactgctta tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt
     9241 tcttgccgat tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa
     9301 caattcgtgc caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag
     9361 atcccgaatc ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg
     9421 aaacgctgga agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt
     9481 gaaagtttac ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc
     9541 agtgaaggaa cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca
     9601 attcaacgag tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg
     9661 acatttggcg aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta
     9721 gacaaggtct tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat
     9781 attacgccag gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg
     9841 gaagatggcg ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag
     9901 ctagctgatg atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca
     9961 accatgatca accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt
    10021 gatatctata tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat
    10081 tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat
    10141 cgtttgatgg ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc
    10201 gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt
    10261 agccatcaaa tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca
    10321 ttggaaggct tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa
    10381 ccgcagattg cagatacgat ttcggatcag atatctggaa atatccatac gattgatact
    10441 gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg
    10501 gtcaatggcg ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca
    10561 caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat
    10621 tatttgcggc tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc
    10681 ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa
    10741 gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac
    10801 agcaagaaat agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc
    10861 gccctgatcc tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt
    10921 tcccagaaaa agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag
    10981 aaatgcaaga agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag
    11041 ctaattcacc cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg
    11101 accatcatcc caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta
    11161 gctgtagtga gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg
    11221 ttaaggctgc acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc
    11281 cctccacgac gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg
    11341 caagtgcttt gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt
    11401 atctttatca aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag
    11461 aaattcttga taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg
    11521 gagtgattga cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc
    11581 gcgtacgttt acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag
    11641 gaggccatcc attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct
    11701 ataaagagat ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg
    11761 ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt
    11821 ccgttacaat agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg
    11881 cccagaatgt ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg
    11941 cgggaacgaa tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt
    12001 agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa
    12061 agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt
    12121 agaaggcgat cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc
    12181 ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac
    12241 taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta
    12301 tggtttttta gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc
    12361 gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt
    12421 gactggtaca caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt
    12481 tcaacaaatt gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt
    12541 gtctcaagca actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg
    12601 cggacatgcg gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt
    12661 tccagcagct aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct
    12721 tagctggaac tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt
    12781 ccaattggtg acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag
    12841 tgaaggcgga ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc
    12901 agcactgact tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt
    12961 attgttcggc ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc
    13021 tgagctaata ccgagagtta gctgattgaa taccatttat ttggaataag aggatgtgac
    13081 aagaattttg tcacatcctc tttgttttgt tctcataacc ttttttcata tacagttcat
    13141 gaaccgcttt tattaacttg ccaaataaca ctgtattgtg cgttgtatcc agatagaaag
    13201 tctccgtttc atctgaaatt ttctcatgct gatctgtaat gatcaaatca gcatgttctg
    13261 aatatgcggt aattttaaca gcatctttgc gaaataactg ctgcaaatgg ctttgaagat
    13321 gataatttcc tgtaaactgt ttggttatct gcaaatggat gagtaactgc tctttcgttt
    13381 ctgtttgtag taagtgttgg atcaattgac taaggtattg actgatttgg tttctttccc
    13441 cattttttga aaaaattgta tcgatttttt ctcttaattt cttttcctgt ataaaagaac
    13501 tagaatgatt ggacgtggct tctggaaagc aaagtgtaga aaaactactg ctggattcac
    13561 ccaacagttc gaaaaaaaca actgccaaca caagaaaata aaaataaatt ggtattttat
    13621 tttctaaatg gttgagagga aactgttgag ttaagtaagt catcaattga ccgacttgtc
    13681 gacaaataag gtttttgctt gtggaaaata aaaaaccaat ctcctgtttt tgtcgaaaag
    13741 agatttgatt tggaaaaaaa caatagaaaa aataattaaa gtaggctctc tctgtttctg
    13801 tctcttcaca agggcgactc actgaaaaag aagtaaagta ttctgaataa tcatgtgtat
    13861 gctgcaataa gtccatcaat tcagtgattt cagaagaagt ttttggaaaa tatgcctgtt
    13921 gtttgtttcg taatatgaat aaagttaaca gcaagtagat atttttttct tgttttgaaa
    13981 ttttttctat ttgctcatct gtactgttca tttttagtgc atttaattca agtgaggaaa
    14041 atggccaatc gttacctcca aatgtatctg aaagaaaaaa gtaaagatag agacggatag
    14101 ataactcatt tccttcaaga gtacagcaat tttttacaga taggatccgt aaatggaatt
    14161 ttattaagga atgattgagc tgttttgtca gtttttcgag ataggagggc gatatataaa
    14221 gttgttcaag taaatatttt tttggtacag cagtttgtgg ataattgatt agtaacatca
    14281 aatagttaaa acgattggat tcttttaggt agcataattt taacttagaa aaaacagcta
    14341 actttttagt ttgatcggtt tctttggttt ggatctctcc tttatgatta gaataaataa
    14401 attcatcaat caataaaaat tgcttgagtg atacatttat ttttcggatc agttctttga
    14461 ttgtccgttt tggaatatgt aagtcattgg ataattcttg taaagaacaa ttattttttt
    14521 gaatgaccat ttgtagaaca agtaactctt tttttcttgc ttcaattagc ataaatacct
    14581 cctttttatt ctgtttttgt atatagttag acaaaaactg tgagtggaaa gtcatttcag
    14641 tctgtgttat tctcacagag tgtcgccttc ttttttatat aacgttattt aatttttttg
    14701 ttaccttgtt tattctattg tttatttcct agggtatttt gtcaagatat tcttgttttt
    14761 aaggcggaaa aattga
//
LOCUS       DASJST010000065        14398 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJST010000065 DASJST010000000
VERSION     DASJST010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.065"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U4B05_001617"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U4B05_001617"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN2050760.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="U4B05_001618"
     CDS             complement(2260..2511)
                     /locus_tag="U4B05_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN2050761.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="U4B05_001619"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="U4B05_001619"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN2050762.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="U4B05_001620"
     CDS             3461..3679
                     /locus_tag="U4B05_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050763.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="U4B05_001621"
     CDS             complement(3968..4480)
                     /locus_tag="U4B05_001621"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN2050764.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="U4B05_001622"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="U4B05_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN2050765.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="U4B05_001623"
     CDS             complement(6933..7376)
                     /locus_tag="U4B05_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN2050766.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="U4B05_001624"
     CDS             complement(7377..8165)
                     /locus_tag="U4B05_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2050767.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="U4B05_001625"
     CDS             complement(8162..9103)
                     /locus_tag="U4B05_001625"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN2050768.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="U4B05_001626"
     CDS             complement(9280..9867)
                     /locus_tag="U4B05_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN2050769.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="U4B05_001627"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="U4B05_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN2050770.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="U4B05_001628"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="U4B05_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2050771.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="U4B05_001629"
     CDS             complement(12784..13719)
                     /locus_tag="U4B05_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2050772.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..>14398
                     /locus_tag="U4B05_001630"
     CDS             13905..>14398
                     /locus_tag="U4B05_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN2050773.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPHFNSEF
                     GHLEG"
BASE COUNT         4032 a         3198 c         2546 g         4622 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gcctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctcatt tcaattctga gtttggtcac cttgaagg
//
LOCUS       DASJST010000066        14141 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJST010000066 DASJST010000000
VERSION     DASJST010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.066"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="U4B05_001631"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="U4B05_001631"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEN2050774.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="U4B05_001632"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="U4B05_001632"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEN2050775.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3872)
                     /gene="iolC"
                     /locus_tag="U4B05_001633"
                     /pseudo
     CDS             complement(2837..3872)
                     /gene="iolC"
                     /locus_tag="U4B05_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
     gene            complement(4029..5045)
                     /locus_tag="U4B05_001634"
     CDS             complement(4029..5045)
                     /locus_tag="U4B05_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2050776.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5147..5344)
                     /locus_tag="U4B05_001635"
     CDS             complement(5147..5344)
                     /locus_tag="U4B05_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050777.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5316..6635)
                     /locus_tag="U4B05_001636"
     CDS             complement(5316..6635)
                     /locus_tag="U4B05_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEN2050778.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7376..8356)
                     /locus_tag="U4B05_001637"
     CDS             complement(7376..8356)
                     /locus_tag="U4B05_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN2050779.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8369..9247)
                     /locus_tag="U4B05_001638"
     CDS             complement(8369..9247)
                     /locus_tag="U4B05_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN2050780.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9263..10063)
                     /locus_tag="U4B05_001639"
     CDS             complement(9263..10063)
                     /locus_tag="U4B05_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HEN2050781.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(10338..10487)
                     /locus_tag="U4B05_001640"
     CDS             complement(10338..10487)
                     /locus_tag="U4B05_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050782.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            11455..11781
                     /locus_tag="U4B05_001641"
     CDS             11455..11781
                     /locus_tag="U4B05_001641"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2050783.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11762..12697
                     /locus_tag="U4B05_001642"
     CDS             11762..12697
                     /locus_tag="U4B05_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2050784.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13326..13577
                     /locus_tag="U4B05_001643"
     CDS             13326..13577
                     /locus_tag="U4B05_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEN2050785.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT         4010 a         3119 c         2393 g         4619 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa agcagcgctt
     3181 tcattatctg acatgttagg ctggatcaac ttctcagtta gatcgaactc ttctcttgaa
     3241 cccatgataa tgtctgattc cttagcaatc atcgaatagt aaattgaaat atcatcgttg
     3301 ttcttccagt tataagcacg gtaatcaata tcaaaaataa ttttgacatt attccttttc
     3361 gccagcatca ctgccttgat tacggcttct ctggacgggc tctctgctag tgcagttcct
     3421 gagattaata gtgcttttga ccgtttcaag tagtcctcat caatatcctc aacgctaagc
     3481 tgcaggtctg cggcttgatt tcgatacatc aaaatattac tttcctttga tgagagcatt
     3541 tcggtaaaag tcagccccag cttttcacca tgctgcgctt tgctgatctg agacgtatcg
     3601 atgccttgtt cattgaaata atcgatcaca tattcgccaa actgatcgtc cgagactgtt
     3661 ccgataaaac cagctttcat gccatgttta gtaacaccca cagcaatatt ggcaggagat
     3721 ccgcccacaa atttttcaaa catatggacc tttttcaatg gtttaaactc ttctttgact
     3781 tgatcattgt aggcaggatt aaaatcaata gcgactcttc ctaaaagcag caagtcaaat
     3841 tcttttgatt ggtcaaactt tatataactc aatatatcca cctctttatc cgttcttcta
     3901 tttctgaggc aagtatagca cgccaaaaca aagcgtgcaa tatttatttt agaacgcaat
     3961 attgcgtgct gtttgaaaat gacggcaaaa aaaacgtgaa tcgccttaca tcacgattca
     4021 cgcctagatt aaatattgta ttttgtctta ataattatat cagtaaatag cgcatctttt
     4081 gtaaccgttt tattattttg cagctgattc gctaacatca gtaatgaacg atagccttga
     4141 acaaatccat cttgatcgat tagaaaatca acctgatcgt ttttcagcat ctcgatattg
     4201 ttcttagtca aatcataaat gattacaaag ggccgcttgc tttcatcgat tttttccaac
     4261 gctcgggcaa ttcccgcttg tccgccagaa aagacgacta cgccatctaa gtttgaataa
     4321 ttctccaagg tgttgcttaa aatcttttca acctcagcac tcgaatcaaa gctgctctga
     4381 acacccgtca gctccagttc aggaaacgac tctttcaatt cttcaacaaa gcctgccacc
     4441 cgtaagctgt tgacgctatt tccaaagtag ccggtaatcg ctagaacctt gccctttcct
     4501 tttgtcagca tacctaacaa tcccgcagcc gtttttccgc ttttgaagtt gtccatccca
     4561 acaaaacact gtcgattcgt accaagaata tccgaattaa aggtaatgat gaggatgcct
     4621 tgatcggtca attgattaat tttttctctg ataccatcac tatctactgg catgatcgcg
     4681 atccctttta ctccaagctc caccaatcga tccaacgcct gcagctgggc cgcttcatcg
     4741 accccgtcta cctcttctaa aaaacaatca atgtttcgct tttttaaatc cccgataata
     4801 gattccagac ctgatcggat cggaatcata aaagaagatt tcgccaattg agtgacaatc
     4861 cccagcttga gcgttgtctc ttttatgacc ttctttttct ttggaacata gttcatttcc
     4921 ttggcgattg ctctgattct ttctgcaacc tcaggattga tccttcctcg atcgttcaat
     4981 gcccgatcca ctgtccccct tgacactcct gctttctcag ctatttcttt aattgtaccc
     5041 gccatcttgc ctctcctttt ttcctattgt gtttatttta tcacaaaact taagaaaacc
     5101 accggagaat gaattctccg gtggactttt tgtatgatta accttcttat tgaacatcac
     5161 tgatcttaaa ctctattcca agggcgtttc tataatattc tggatacatc tccttcccac
     5221 atttttcaca ggtaaattgg ggaggattgt tcggatcatt tgcaatatct gcatcatcta
     5281 aaaattttac tacatccatt gggattgatt ctttttcacc acaacttaga catataaatt
     5341 tcacgttggc gttcccaatc ccagcgaatt gctttaaaag ttttttcttt ttcttcttca
     5401 tactgttgcc tgaattcttt ttggtcaatt tttcgattct cctctcgcat aaaccgcttt
     5461 gcatggttat ctttcgcata tttaataact aaccgtccat ttttgcgcac actcatccca
     5521 acgaattcat agtactcacc gcaacaagaa cattctaagg gattttcacc aaattcttcg
     5581 gttattcttt cttcccagga ttttggtctt aaggcctcat tcagattgat aaacagcttt
     5641 tttactcgtt tctgatactc cttcatgacc tttttcatta atgttttgat tctacgacta
     5701 tagattccat agcgacgaat cgttttgaaa ttcttatttt gaatatggcg aatcagtgca
     5761 gctatgaatt cgtctatact catggtcatc gtctctttag tattcgttcg tttgtccatg
     5821 tagttgaaca tgacagtgtc gccatcatac attaagattc ggcttaatgc gatcgggcca
     5881 cgcttcatat aacgactgat gtattggagt agtgacttta gatttgtcct gctcctttta
     5941 ggagcattta cgtaaaaccc ttctccattg ttcttgtacg cagcttgtaa tcttgattga
     6001 actctttgct tgtcccactc acctaacgtt cgtctaatta gtttcaatac agcatttttc
     6061 cagtattttc ttaacataga aaaaggtaaa taatcgtatt cttcccaact tccatctctt
     6121 gttaagccac ccatagtaac aaccatatga acatgcggat taaattctag ttttgatcca
     6181 aaggtgtgga ttgtagctac tatacctgcc ttcacctttc ttttctcaaa ataggcaaga
     6241 ataatttttg cggcttcatc cattagtcct ttcagtaagg aatctcgata gttttccatc
     6301 ataaagattt cccgtaaccc ctcatcgatc gtaaagatta cgtgccgatg tgtcacttta
     6361 aacatatcat tcgccacaac cgctgaccac ctttggcttt cacctgttgc acacgttgga
     6421 cagaactttc cacgacattt caagggaaca aactttgttt gatgacatcc ctcgcagaca
     6481 aacagtcggt atcctttttg aatatctcca caatatctaa acttgtttac ttcccgtttg
     6541 acgactagcc gaatctttcg cccgtatttc tcaacaaagt tatcccaatt atggtgttcg
     6601 tcaaaaaaga ttgcttcgag aatattcgct ttcatagtaa tattttactt gttcaaaaac
     6661 aaaatgaaaa gggctaaaag actggtagtg cagccttttt agcccttaac tttttatact
     6721 ttctttactg tctaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt
     6781 attcaaattt gtcttcgcca acacaatttg acgaagagcc ttttctttca ggaagacttc
     6841 ggtaattttt aggagttcta cctattcttt taaccgttta ttctcttttt ctaagcgtgt
     6901 aatttcattt ctgcgaatgg atttcatgac ttcctctacc gagaaaagta cgttatccat
     6961 atttttcaac ttcttgaact catcaataca cgctattaga gcgagcttct agctatagac
     7021 taacttcttt aacagaaaac ccttcttccg ttaccagttt tacagcggta attttaaata
     7081 tttcatcaaa tgaacgtctt ggcataatcc agatcttcgc tttctcttaa tttggtgtca
     7141 attttagtat aacccgtcca acttattttt taactaagtg cccggttttt cttgacaact
     7201 ccacccaaaa gttagatttt ttggtctaag ttttagggtc gcttcattac tagaattcaa
     7261 caagagatgt tttttatttt atttaaatta tggggtcagt ttccatttga ttacaaactt
     7321 ctttgttttc tttttttaaa cgcaataata aaggttttaa tttcagcctt tttaattaga
     7381 aaatcaattt tcccaaatta ttttctttag ctttcttaac gatttctgtc gcaactacca
     7441 catctaatac agccgaacca actgttttga agatcgtgat ttgttctcca cttgtccgac
     7501 cgatcaattc ttcattgatc agttggccta gttccccatg atagctgtct ctttgaatat
     7561 acccatcttg cagcggtgaa atgaagtccc cagcttcttt tagaacaccg tccatggtat
     7621 caaaaataac aacatctgcg gctttgatta tttctcgtgg aatttcacac atttccggtg
     7681 tgtaagcacc gactccattg atgtgcgcac cttttttgac ccactcagca gagaatgtcg
     7741 cacgttttga agtggtcaca cttgtaatga tgtctgctcc ttcaacgcat tcttgactgg
     7801 tttttgttgg gcgcatcgtt acagaaaatt gttgcatcat tttttcagca aattggctgg
     7861 cacgctcaaa atcaatatca aaaatccgaa cttcttctaa tttacgaacg gtcaacattg
     7921 cttctaattg tgactgtgct tgtccgccgg ttccaatcag cgcgccaatt ttagcatctt
     7981 ctcttgcaag aagttctgtt gctgctcctt gaacagcgcc tgttcttaat tgtgtcaaat
     8041 aagtaccatc caaacaagcg gataccatac ccgtttctgg atccaatacg atcatcgttg
     8101 ctggtacact tggtaagttt ttcttgatgt tttcaggata cacggaaacg attttgacac
     8161 ctagcgcgcc ttcgccacct tctacataag caggcatata taagctttgt ccgttgcttt
     8221 ttggcacatc gatattggtg cgaagaggca cacttgcctt tcctgctgaa tacaatgaca
     8281 gtgctttttt gtccgcatcg atggcttctc tcattgaaaa acttttgata atatcttctt
     8341 ttttaagaaa taacatttga atcgctcctt aattagctgc tgtaactttt tttaattgac
     8401 tgcccaacat cgtatatgca ctgtatagta agaccaaaat cacgatccat ttcaggatat
     8461 aggtatctaa agatttgact aggaatacag cgaccaatac ccccaataca ccgaaggttg
     8521 aagtaaaaag tgtgattttt ctgctgtatt cattaaattt aatgaattga acactgccga
     8581 tcggtactga gaaggtacaa gcccccatca taataggaaa ggctgcaact ggattcaagc
     8641 ctagcaagta gacagttacc atcgttaacg cataagaacc aatgccgata ttgttcaatg
     8701 caccatagac aaagagtaaa aatcctgcca agattaattt tccgccatac aattcactgg
     8761 ctgttccgtt tgatggaatc cagttcattt gtcctgcaaa aatcaacaat gcagcaatga
     8821 ccaatcctat gccgacaaac agtttgatcg ttttctctgg taacttcacg acaaatcttg
     8881 gtcctaaata tgcaccgata atttggcaga taatacaaac agctagtgtc ttgatcccta
     8941 cgttgatggc agtgatgtaa gacaatgcca tgacggctac cggtactaca cattgggtat
     9001 tcaacgttcc gggtagtttt ttcatgctga cccatttcaa ctttggataa aggacggttc
     9061 cgatagcaaa gtcggaaacg ccaaatgttg aaagaaagaa gatgataaat gacgtgaagg
     9121 gcaacagacg catatcccct ggttcttctt tgaaattttg tttgtttttg aaaaggtctc
     9181 ttagaaagta aataataaaa tatagattga ttgcgacaat tagtgtcagc aaaatattcc
     9241 gaatcataaa aaattcctcc tactagtaaa tctttctgat tccctcaaag ccctcaccat
     9301 attttgcttt aagttcttca ggagattctg caatcacttt tagtgtatat gcatcatgtt
     9361 tgacatattc ttttgctaga ttgaccatgg cgtggttttt tgtccaagtg acatcggttg
     9421 attgcagttc ttcgttaatg gtaccaaaca cgacttcttg ctcatcggcg acgatgttga
     9481 tccatcttgc tccaaagtca gcaagtttgt ctgtttcaaa accatgaata taatactttt
     9541 ctaagggaag atcatattca tgggtagaac tgaataaaat cagtacaaac ttatcaactc
     9601 ttttttcagc ttgctgcaat gcctttagta gtgaatccgt gaaattctca tgccaaactt
     9661 gaatgagcaa actttctttt gcgtttttca ccaaatgctc tactttatcc agaacatact
     9721 cgataccatc aactttccac agcatttctt cttcatcttt ttctttgatc atccctagat
     9781 tcttggtaag aaaagaaaga tcatttttaa cacttttttc taatttctct aaaaactcat
     9841 ttgccgagat ggcatgataa agtttaggtt ctgatttatt aacgacaatc aaattctttt
     9901 ttaatagttt ttccaaaata ttatatactt tcgagcgtgg cacacctgag attttactta
     9961 cttcatatcc tgtaagatta cctttttcta atagtgtggt atacactctt gtctccatct
    10021 ctgaaaaatc atattttttc atgatttgaa taatcgtttc cattgattaa tcctcacttt
    10081 atttttagta gctactacgg taacaactat aaattctttt tgattatctg tcaagagtta
    10141 gttcaataac acacatataa taattagctt aaaaacaatc tttttattaa aaagaaacat
    10201 tataatatga atgtttgtga aaaaataata caacttagtc attctttaca acgatttata
    10261 gaaaaacaac ctctttaaat ataaaaggta cgggttactg attacttgta aatctttacg
    10321 ttccagtttt attctttcta aaaaatatac tcacttcttt tcttagactt ctttttttga
    10381 ttctcctctt tatggtcttg tccttttttg ccttcaaaat atttaaataa acccctttca
    10441 aaagcatttc taggttttgg acaataagga atgacttttt tcttcatata aatacctcat
    10501 ttaactattt gatatcatca cattatcaaa aaaaataggt tttttattaa aactatcttg
    10561 acctttcggg agatttcact gtgatttatt tttattccca tacttttttc gttggtcact
    10621 ttcgtatacc taatctaagc gatcatcggc gacggtgctt ttacaacgaa tggcaggaca
    10681 aaacaagggg cggaaaaact tttttgccta tttcataaaa aactttttcc tacagcaatc
    10741 ccttgttttg tcctaccaca cgttgtgaac ccaaagcacg ccgacgaacg cttagctata
    10801 cgaaaatgac ctccaaaaac aaaaaagtga ggtgaataga aacaaaaaga aattgaaatc
    10861 tcccaaaatg tcgtccgcct cggagacaac ttgctgctga aaatggttgg ttaattcatt
    10921 taagtattaa ataagataaa tcatcaaatt aatcggaaaa atagcatgat tggccttatc
    10981 aaaaataaac tgtaaaaact tatatttcta tgagtggtgg aacaatgcta ataccaaatt
    11041 aatttatcga ttacccacct gcacaaacca aagatagcat tattaggaaa cgaatcaatc
    11101 agcgaatcca gcattataat gaggatacta aaaataaagt tttcataatt ataaatccgc
    11161 cttcctacga aaggcaccaa tgaggtgatg aaaaccttag agcgctttct ttcttttttt
    11221 attccgtgtt accctttcct tgcaggtccc tggtacacta cagaacccga ggaggtgagt
    11281 ggcggggctg tatagcggca accccgcata attatatggt gtcggtcatc cgttaaggca
    11341 tcaatgaatg gcgcaaaccc gtagcccgta aacttatctc ttccgaagtt gactcgattt
    11401 cgccggatga ccagtcccta ccaaacctgt actatttttt tacaggaggt tgctatgttc
    11461 aaaatctttc gtaaaaattg ttgtggcttg gatgtccaca aaacgtggat ctatgcgtgt
    11521 atcggaatca cagatgcgaa tggtcgtaca gtgtataagc aagctcgctt ctcttccttt
    11581 acaaaaggtc tcaatgaatt agctgaatgg cttgctaaat acaactgtaa ggatgtgtgt
    11641 ttggaatctt ccggtaaatt ttggattcct gtttttaata ttcttgaaaa aacttgtttt
    11701 gtcacactgg ctcatccgaa gtacaccaaa ccccagaaag gaaataaaac agatcgcaaa
    11761 gatgcaaaaa tggatttgtg atctctttat gtgcgacatg atcaaaccca gttttattcc
    11821 tggtcttgaa atccgccagc ttcgtgatct aatgcgttat cggacgaaac tgaccaacat
    11881 gaaaacaagt gaaaagaatc gtgctttaaa ctgcttaacc gtttctaatc tgaaactaga
    11941 tgatgttttt agtgatgtgt tcggaaaatc atccacttct atcatccaat acttgttaga
    12001 acatcccggt gaatcatttg atgttcgacc atttatccat gcccgttgta aaactcccgt
    12061 tgaagaaatc caagcggcag ttgagggggc gatttctgta cctcaagcga ttaagcttcg
    12121 ccaatgcctc aaccatatta acgagattga gaagcatcga gaagagatca atctagagat
    12181 tcaagctctt gcagaaccct tctcccctgt tttggaacta cttcgtacca taccgggctt
    12241 agacactcag ccaataacag cgattgcgat tctttcagaa ataggaccgg atatgtccgt
    12301 gtttccaact tcgaaacact ttatttcttg ggctggatgc tgtccaagaa acgatcgcag
    12361 tgcccgacga acaaaatcaa cacgtatctc tcgtgcaggg cagtcgttga aaccgctact
    12421 tgtacagatt gccaatgctc ttctcagatc caaagaacat ccagaattta aaaatcgcta
    12481 tcgtagaatc aaagcgaatc gtggacacaa gaaagctatc attgctattt gtaagatgct
    12541 cctgaccgct atctggaatg tcttatcaaa ggtggaaccc tattctgcta aaggatatct
    12601 ggagccaaaa cctgcgaaag aagagaaagt tctgacgcaa tcccaagctc tggacctttt
    12661 aagaaaacgc ggatatacca ttatagatag tgcttaaacc aaagttacgt tgattcttcc
    12721 taaatagact cgttgtcttc catttcaaaa attttgaaaa cggtaggctt atttagtgta
    12781 ccgttcttca ggtatcttca aatagattta ttttcaaact tatttccttt cttcttttac
    12841 tctcctatag cttagcattt aagtgggcac tcgtgtcacc ttgctagata gctatgaatg
    12901 ccgtaataag cctcctagta tataggcaga ctgccaatta taactgattc aatccacatt
    12961 atgataaaat ccaccttttc gtttcatatg tgttgaaata tcacatatgt caaaagtgca
    13021 cgagtctttt cattcagtgc tttgtaaata ctcaaaatta ttccttttca ctttaaaaaa
    13081 aagataatta gtacaaaatc agaaatgagg aaagtattag gatggactag aaaggagttg
    13141 ttgaatttgt tgcaacagat tggtcattta tcaattaaaa attaggtgaa gttttattca
    13201 tcactgcagc tgctgtttcc cagagaataa aacgtctaga agaaactggt cagttactgt
    13261 attatcaagc agcaattgac tacgaacaag cttgattact aaccaaagcg tttatccacc
    13321 tgttgattaa atcaaatcaa aaatcaggtt tttatacgtt cataaaagca atctccagtg
    13381 tactcgaatg tgactgtatc ataggtcctt attcgatgtt aatcaaaatg gggtttcctt
    13441 ctaccgaatc attggacagt tttatcaatg atttatcaaa gtttggacga accaacaccc
    13501 aaatcgtttt ctcgactctt gtttccttaa tggtttatca gttctggaat gtttatcatg
    13561 cgcttttatt aaaatagaaa agagcttttt tcatgcgaca aagcagtgat catttattgg
    13621 cacatgcaat ctttgtggat ccattttttg aaaatacgat atttagtttt gacccaaaaa
    13681 tctttaaata cgtatttgat aatttatctt aacaaccaga taattttatt cagcaccgta
    13741 ttatctatat tgaattctcg gaatcaagat aaattttttt ccgtttttgt ttctttttta
    13801 atcttcttct tgttattaat agtgattagg atgatattcc tatattgatc tttgataatt
    13861 agatataatt tttgaataac gttccctgct attcgagcta gaaatacaag tacgccatga
    13921 cctagttagc gacaactact tagagatttc ttttgttctg ggacaacaaa atgccatgac
    13981 aatagcagta aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt
    14041 cctatgtgct tacccaaagc ggttcaaaaa ttttaaacta ttattttagg agatgatttt
    14101 ataaaatcga aagcctactt gaataaaaaa caagcctgtg a
//
LOCUS       DASJST010000067        14036 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJST010000067 DASJST010000000
VERSION     DASJST010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.067"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..268)
                     /locus_tag="U4B05_001644"
     CDS             complement(26..268)
                     /locus_tag="U4B05_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2050786.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(300..857)
                     /locus_tag="U4B05_001645"
     CDS             complement(300..857)
                     /locus_tag="U4B05_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2050787.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(870..1058)
                     /locus_tag="U4B05_001646"
     CDS             complement(870..1058)
                     /locus_tag="U4B05_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2050788.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1071..1637)
                     /gene="amaP"
                     /locus_tag="U4B05_001647"
     CDS             complement(1071..1637)
                     /gene="amaP"
                     /locus_tag="U4B05_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2050789.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2054..2227
                     /locus_tag="U4B05_001648"
     CDS             2054..2227
                     /locus_tag="U4B05_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050790.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            2867..3340
                     /locus_tag="U4B05_001649"
     CDS             2867..3340
                     /locus_tag="U4B05_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN2050791.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3349..3576
                     /locus_tag="U4B05_001650"
     CDS             3349..3576
                     /locus_tag="U4B05_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2050792.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            4212..4583
                     /locus_tag="U4B05_001651"
     CDS             4212..4583
                     /locus_tag="U4B05_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050793.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(4839..5081)
                     /locus_tag="U4B05_001652"
     CDS             complement(4839..5081)
                     /locus_tag="U4B05_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2050794.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(5113..6015)
                     /locus_tag="U4B05_001653"
     CDS             complement(5113..6015)
                     /locus_tag="U4B05_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2050795.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(6028..6216)
                     /locus_tag="U4B05_001654"
     CDS             complement(6028..6216)
                     /locus_tag="U4B05_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2050796.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(6230..6793)
                     /gene="amaP"
                     /locus_tag="U4B05_001655"
     CDS             complement(6230..6793)
                     /gene="amaP"
                     /locus_tag="U4B05_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2050797.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(6831..7724)
                     /locus_tag="U4B05_001656"
     CDS             complement(6831..7724)
                     /locus_tag="U4B05_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2050798.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(7802..8725)
                     /locus_tag="U4B05_001657"
     CDS             complement(7802..8725)
                     /locus_tag="U4B05_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2050799.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(8774..9124)
                     /locus_tag="U4B05_001658"
     CDS             complement(8774..9124)
                     /locus_tag="U4B05_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2050800.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(9157..10059)
                     /locus_tag="U4B05_001659"
     CDS             complement(9157..10059)
                     /locus_tag="U4B05_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2050801.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(10052..10909)
                     /locus_tag="U4B05_001660"
     CDS             complement(10052..10909)
                     /locus_tag="U4B05_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2050802.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            11243..12052
                     /locus_tag="U4B05_001661"
     CDS             11243..12052
                     /locus_tag="U4B05_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2050803.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            12092..12589
                     /locus_tag="U4B05_001662"
     CDS             12092..12589
                     /locus_tag="U4B05_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2050804.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(12974..13047)
                     /locus_tag="U4B05_001663"
     tRNA            complement(12974..13047)
                     /locus_tag="U4B05_001663"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13011..13013),aa:Ile,seq:cat)
     gene            13234..13557
                     /locus_tag="U4B05_001664"
     CDS             13234..13557
                     /locus_tag="U4B05_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050805.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            13721..13975
                     /locus_tag="U4B05_001665"
     CDS             13721..13975
                     /locus_tag="U4B05_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050806.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT         4053 a         2623 c         2317 g         5043 t
ORIGIN      
        1 caacataagt ttttcttcaa tttgcttata atttacgtgc aaagaatgaa acgactaata
       61 ctaaaatgat tgcaccgatc aatgatggaa tgatagccat tcctgcaatt gttggtcccc
      121 aagttcctag taaagcttgt ccaattgctg aacctactaa accagcaatg atgtttgcga
      181 tgatccccat tgatgatcct ttattcgtga ttgctcctgc aattgctcca atgataccgc
      241 cgacgattaa tgcccaaata aatcccattt taaattcctc ctaactgact tcttacttct
      301 tattgtacac gtggttcgtt attttctttg actttttgtg cacctttgcc tacagcagat
      361 tttgctttat ctgtttggcg tgaagcaaat tgtcctgtgg attttgctgc ttctgttact
      421 ttgtcttgta ctgtttcttg atctttttgg tattcttctt gtgttttgat gtctgcaaca
      481 ttcacattca cttcgataac atctaaatgt gtcatattgt ttacttcagt actgatcaat
      541 tctttgattt ggttgtaaat atcttcgatg tctttaccgt attctacgat cacatctaga
      601 tccacagcta cttgtttctt acctacttct gtattgatac ctgctgttac gttatctgtg
      661 ttgactaatt tttcagccat atttgagaaa aatccgccat ctaatgtcaa tagaccatct
      721 acatctttca atgcataacc gatgattttt tggatgactt tgtcttcata agtcaattca
      781 ccattcatca tgcggttttc tcctttttca tttgccaaaa ctgatccatt tgttgtattc
      841 atgtttttgt tttccatctt tgtttcctcc tatgatttaa aaaattgatc aactattcct
      901 gactgtttca aataaaaacc gattcctgcg ccaattgctg tcatgattat gatcaacaat
      961 gtcttgaaaa atccaatcgt taaccataaa acagctagca gtagtccaag acccccaaag
     1021 ataataggga gcttgtatgc tgaaaatagt tctttcatta tttggccctc ctattccact
     1081 cgagagtgct tgtagtctct tgtatcttct tgttcttcat aaccgctgta tgtgactgcc
     1141 actttgaccg tttcttttgt tccgagtact ttagttactt cttcttcgat atcttgcatc
     1201 aatacacctg ttttccctac taatgaagaa gttcgtttca agtctccgtt gacatggata
     1261 tggattcgat ttttcgttga acgtatccgt actttaggtg aatgaatgaa ctcttcttcg
     1321 tgtaaatggg aacgtaccaa cccttcgatc gcttttttgt cgattgttag tttgccatct
     1381 tcccgtttca taacgaaagt tcctcttgac tttggataga aaagaacgac tagcaagatg
     1441 ataatagcaa gaacggcaaa tactacagct gcccagaaaa tatattgttg catgtagtaa
     1501 ttcgttaaag taaagaagcg aaatcgttcc aattgttcag gcataatata cggcgcattg
     1561 atcaacgcaa tcgctagaaa tactgaaagt agcagtaaac tcatgataac accgattatt
     1621 tttaacccct tattcatttt ccacctccta taaacttatt tttaccctgt aaataactct
     1681 accgacaatc acagtataga tagcgaacag ttagcatagc atcagtaaat aaagcgctac
     1741 caaacattgc tggatcataa cggcgtttta ttgccagtct gatactgaaa taacaattgc
     1801 ttacttattc tctatttact tttgattgct ggctatactt aaaggatatt attcaagatt
     1861 aaaaattgca aagattatgc tcagttatta ttacaagatt ctatttattt attaaatttt
     1921 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
     1981 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
     2041 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
     2101 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
     2161 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
     2221 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
     2281 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
     2341 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
     2401 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
     2461 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
     2521 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
     2581 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
     2641 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
     2701 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
     2761 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
     2821 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
     2881 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     2941 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     3001 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     3061 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     3121 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     3181 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     3241 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     3301 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     3361 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     3421 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     3481 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     3541 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     3601 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg cctattattc
     3661 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
     3721 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
     3781 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
     3841 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     3901 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     3961 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     4021 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     4081 cattattttg ataataatga ttatttattt aaaaatacat tagataacat ttgttacgac
     4141 acgaaaaagc ttcacaaaca aatatcgtta ttttaaaatg agggcgaact cacacaatta
     4201 ggaggaatag tatgccattt attgtttact tttttctttc attactcgtg atctatattc
     4261 cattaccaac atttttcctg ctcgtcaatc gtctagcaga gcaacatgat acgattgctg
     4321 ctcttacgaa actttgttta cttctatttg tttgtctaga ttactttacg agctattatt
     4381 ggctaaaaca gacaagagta aaaaagagat attatttctt ccttgtcttt ttcaatctct
     4441 ttgagtttct gatccattgc tttttacttc atcagtatca tacgatccat ttgtggatca
     4501 tctgggtttt caaagtggtc gtattatttt gtatgttcac ctttcctatc tcaagaaaat
     4561 ttagggaggc attgttttat taaagaagct tctctgtatc aataaagaca catttaagga
     4621 aaaactttta aaaatctaat tgggaatttg cccgcataaa taagaggctg tgacaaaagt
     4681 cttgtcacag cctcttattc cgtataaaca gtgttcaaaa gcttgcatct tcgataatat
     4741 gcccaaccaa atgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg
     4801 aagaacagct ttttcacgac ctctttttaa aagtcttttt acagttttcg agcaaagaat
     4861 gatacgatta gtactaaaat gatggcacca atcaatgatg gcacgattgc cattcctgca
     4921 atacttggtc cccaagaacc taataatgct tgaccaagtg ctgatccaac taaacctgct
     4981 acgacatttg caataatccc catcgaagaa cctttattcg tgattgctcc tgcaattgct
     5041 ccgataattc ctccgacgat aagtgaccaa ataaatccca tgtttcgttc ctcctaatgt
     5101 tttacttcct tattactgaa cccgtggttc tgtattttct tttacttttt gtgcgccgct
     5161 tccagctgct gattttactt tatctgtttg attcgaagca aattcaccgg tttttttcgc
     5221 tgcgtcagtt actttgtctt gcaccgtttc ttgatctttt tcaaattctt cttttgtttt
     5281 gatgtccact acgttagcgt tgacttcgat cacttctaaa tgtgtcatat tttttacttc
     5341 tttgccgatt acttcttgca tttcttcaaa gattttttcg atatctttgc catattccac
     5401 aatgacatcc atatcaactg ctacttgttt tttaccgact tctgtttcga tcccagccgt
     5461 tacattatct gtattcacaa gttttcctgc cacattagag aagaaaccac cgtttgcaga
     5521 taataaaccg tctacttgtt caattgcgat tccgatgatt ttttgcacaa ctttatcttc
     5581 ataattcagc tcaccgcgca ttgaatcttc ctgttcagaa tcatgctttc cttggttatt
     5641 tgtatgatca gaaccaagtt ttccttgtcc gtttgtgtgg tcagaactaa gtttttcttg
     5701 tgctttcgca tggtcagaag gatgtttttg ctcttccaca ggtttagaag gatcgattgc
     5761 atcatgttgt tctggaactt tagattctgg agttcctgga tgatgaggtg catgcttttc
     5821 tgcatcatgt ttcttctctt ctactccttg ttgaacgtta gggactactg gatctggatg
     5881 tgcagatact ggtgctgttt tgttcttttc atcgttcaca ccatttgatt ggtttggtgt
     5941 cgttgggtct ggatgtgcag acactggttt ttggttgttc attttattgt tattattttt
     6001 gttattgtta ttcatggatg tttcctccta agatttaaaa tagtgatcta agattttcgt
     6061 ttgtttcaga taaaaaccga tagcgacgcc gatcgctgcc atgatcaaga tcaataaggt
     6121 cttgaaaaag cccacggtga tccataaaac ggctaatatc aatccaagta ccccaaagat
     6181 gatagggagc ttgtatgtcg aaaatagttc tttcattttt ggtccccccc tattctactc
     6241 gtttgcggtc attgcttcgt ttagctggtt cttcaaagcc tgtgtatttc accgatactt
     6301 tcactggctc ttttgttccc aatatgtgtt gtacttcatt ttcgatttct ttcatcaatg
     6361 ttcctgtttt accaatgagt gctgaagttc gtttcaactc cccttttaca ttgacggcga
     6421 tcttgttttt cgttgctttg acatttactt ttggtgaatg gatgaattca tcttcatgca
     6481 agtgagaacg aaccattcct tcaattgctt ttttatccaa cgtcaactcc ccatcaccat
     6541 gtttcagtag aaagtttccg attgtttttg gataaaacag gaaaacaagt aacgtgataa
     6601 ttgtcaaaat agcaaatgca acggcaagcc aaaatacata ttgttgcacg tatgaattag
     6661 tgatggaaaa gaatcgaaac ttctctagct gaaaaggcaa tggataggtt gcacttaata
     6721 gtatcgcaaa caaaagcaca gatagcaaaa gtaatgttcc gatcactccg atcgttttta
     6781 cacctttatt cattttcgca cctcctcata ggggtgaaac gatcaacttc ttataagttg
     6841 atcgcttctc caccagttac tccgtaaatt tgggctgtaa cataacttgc tttattcgat
     6901 gctaagaaaa cataaactgg tgctaattct cctggctgac ctgcacgttc tagtaaactt
     6961 tgctgaccaa attcagggat cgaatcagaa ggttgtcctt cgtctagttg taatggtgtc
     7021 cagattggac caggtgctac accatttaca cggattcctt taggtgcaag ctgttttgct
     7081 aaaccgatcg taaagttagc gattgaagct tttgttgctg cgtaatctaa taaatgttcg
     7141 cttggattga atgcttgaac agaagttgtt gttacgattg ctcctcctgc tgggatatgc
     7201 ggaacagctt ctttcactaa agcaaacata gcgatgatgt ttatcataaa tgtgtctttg
     7261 acttgctcaa gtggtaactc agcaatagac tgacaagaaa tttgctgtcc tgcgtttagt
     7321 accatcgtat ctagcccgcc aagttcactc acggctttat ctatgatttc tttaggtgct
     7381 tgttcgtcac gaagatcaaa aggtagtaag acaatcttac gtcctgcatc tttcacgtat
     7441 tttgctactt cctctgcatc tttttcttct cctgggaaga attgaagagc gatatctgcc
     7501 ccttctctag caaatgcaat cgctgctgca cgaccgatcc ctgaatctcc accagtgatt
     7561 agtacacggc ggttttctag ttgattattg ccttcatagg attcttctcc acaatcagga
     7621 acaggaatca tattctgctg taaagctggt gtatcctgat cttgtttagg aaatttctcc
     7681 gtatggtaca agtttcgcgg gtcgattaat tttggttctg tcattgctgt attcctccta
     7741 gtttttaaat tgtattgcca aaacaaattt ctttgtttga tacctgtttt aaatagattc
     7801 tttaatttgt ataatctttc cggtataaat ggtaacctaa aactactgct gctatcaaag
     7861 agccaaagac tacgagtata aagcctagag cagaactttt acccggtaat cctcctgtat
     7921 tcattcccca aatccctgcg aacaacgccg gaacggaaat gattaatgca gctgaatcta
     7981 gatatttcat gatatgattt agattattgt ccatcatccc gttgaataga tcaccaatac
     8041 tctctaataa atctcgatag atctcgatca ttttttctgt ttgtcgctgt ctcaaatgga
     8101 catcatagag tagctgttca tcgtctaaac gtttgatgaa agtttcagaa ttccataatt
     8161 gatccattgt ttcccgttga tctcttaagg tatgatcgat atatacgatg tctctttcaa
     8221 gatctgcctg tttatacagt tcttcatttt ccgttgtttt acgagctgct ttatccaaag
     8281 cgtcaattcg ctttttcatc tctcttagtt ctttcacgta atgggtgtaa atatctaata
     8341 atgtatacgt gatgatcttt tcaaacgagt ttaaatgatt tccatgcttt gtcagcagcc
     8401 gatccacaaa ttcggttttt ctatcgatac aagtaatcaa gagattatcg gaaagtacaa
     8461 aagatattgg cgttaatttt ttttcgatac gattatctga ctgatctaga ttaatcacta
     8521 ccaaagaaaa tggatgctct agtttcgtct cataaaggcg ctccatccga gaaacttctt
     8581 ccggatattc ggttcctacg aaaatatcat caggaagctc gaattcttcg attgcttgct
     8641 caatctcttc gggctttgca ctatcaagta cgagccaatt atattttttt tcatctgttt
     8701 ctattacatt cttttcttca tccacaaagt agtatctcaa tgtatacctc ctttttggac
     8761 tttctcgaaa ctattagctg aagaagcagt tctttaattt gatattataa tcgagtactg
     8821 cgatttcatt ttctaatgaa aagctgtcaa gctgtttgtt gaagtccatg ttcaatcgtt
     8881 taccgaaata attttgctct aatttagatg tatagatgtc caataatgga tgctcgattt
     8941 tagtcgcata ttctccaaat tccgaaacaa aaggaatgat ttgaaaaaag ctgccttttg
     9001 caaaagcacc tttgtccctt agaaatggat cggaatcatt gataagcgcg agtgcgattt
     9061 ttgtattttc cccaaccttt tcaattaacg tctctactgc atttttagac attgtaattt
     9121 ccattcctac tccccctctt cagttgttgt cttctattat ttaacaacta catctgtttt
     9181 tggttgaact tttgcatatt ttggatattt agggaattta gctacacaac ggaaagccgt
     9241 catccctgca tatttttcgt aatatttctt cgttggccct tcaaaatcct tgctccacgg
     9301 tttcacatgt cctgaaaaat ggataatgta aggattgttg cgagtttctt cgtattcacg
     9361 ttcacctgct gctgttggat gtttttttgc ttttgccatg atatagcctt gtgcattcca
     9421 acgcggatga agtggtaacc aacggtcatg taagatagca tttaatgcat cttggtcatg
     9481 gaatcgtaat ttttctggat tatgttcgat gaaatctaat actttttgcg taatattttc
     9541 atctaaccat tttttcacat tgatcagcat caaacctgag ttgaaataac gcattgattt
     9601 agctgaaatt ttcatttttt ccagtcgttg atggaaccca gcgtcttcaa cagctgctac
     9661 gattgaatca ccgaagtcaa gacgccataa tttagagata tcttgtaaag cgatcatgtc
     9721 gcaatccata tataagatac gttcaacttc tgtgccgcgg aacaattccg gaatcgcaat
     9781 acgataataa gctgtttctg gaatacgatc gctgattacc acatttgtaa agaattcttt
     9841 attgattttc aagaattcca catctgagtt cattcgtgcg tttttcactg agaagcgtaa
     9901 aagctgttta ctttcaaaat cgatatcatc gtcaatcaca tagaaccgaa caaatttaga
     9961 cgggttgcaa ttatctaata ctgagacaaa taatgcagct aaatgtggta cgaattttgt
    10021 gttacaactt gcgactactg ctatttcttt tttattcatt gatctcgcct actttcgtta
    10081 gagtactgtg tgctaatttt tttaagtata aattggtata cggatgatct ttcaacgtat
    10141 tccaaggttt atcatgtcct gtaaagtgaa cgatcgaagg tttttcattt ccttctttat
    10201 actgacgttc atatttttca tttggtgctg gatgacgttc aaaaatcagc gaagtctgca
    10261 tgttccattt tggatgaagt tgttcccaat cttcataaag tacagcattc aaagcatctt
    10321 ggtcgtgata gatgattcga tcgccatttt cactgaggta atggatagtt ttttctgtaa
    10381 tttctttttt attccattga tcaatgtcaa tcaccatcac tcctgaattg aagtacaagt
    10441 catcggaatc gattcctaat ctttccaaag ctttcacttg gcctggatcg ataatagcgc
    10501 cgattgtttt tccatttaac ggctcatcat atagttttac gatatcgtct agtactaaca
    10561 catctgaatc taagtaaagg actttcttat aatcctcttt agctaataag tttggtaaag
    10621 aaattcttaa gtaagcggta gttgtgatat ggtcactgac taggaagtct tcgtagatat
    10681 ctttttccac tgtcaggaat tggatacttg cattacttga atatttattt accgtttctt
    10741 ccaatccttc tttactatat tctgatagac catcgtcaat cacataaaat tttatgcgtc
    10801 ttgttttatt gcaattttct aatgcagttg cgatcatcac gctcaagtaa ggagcataat
    10861 tttcgtctga agcagttacc acaggaacta cgccgtatct cttctccatc aaaatcactc
    10921 ctttttctaa tctttttcta acctgttcta catctagaat attcctcatt tggttttaaa
    10981 gcaaaatata tgctttgttt tcattgaata ttttaaaatt tttttatcac aaaacgcatc
    11041 ggtaagcgct aactattcgt ttttcatggc tttttattca aaggtattga ccaaaaacta
    11101 ctatatatag ccaccttttt cttaagaatg ttttttgaaa aaattcataa tgacaaagca
    11161 agttagtttc atttttttaa attctcattt aatcttaagg taacaggaaa atagatatgt
    11221 aaaaagaaag taggaagata aaatgaaact agcagcgatt gatttagacg gtaccttgct
    11281 tgatcgaaat ggaaagatac cagaagaaaa tatccaagca cttcggaaat ttgatgaaaa
    11341 tggcggaatc gtaaccgtcg ctaccggaag gaacagcatt tctgctaaag atattttttc
    11401 cgaattagga atcggtggct atttgatttc ttccaatggt tcactgattg ctgaaatgaa
    11461 ggatggaaaa atggcgaaag ttctcagacg ttcaaaaata gatagcgata cactgagaaa
    11521 agccttcttc tatgctcaag aagcgaaaat atccatcatt gctagccgag aaacccaaga
    11581 tgatcagctg acattcgatg aatcaaatct agtcaaagat gacccttatt atgaacattt
    11641 caatttacag aaccattctt ttgatcaaat tttaaatatg ttggaagatc cttctcttag
    11701 ctatcttaaa ttagcattaa cagataagag tgaagaaaaa ctacagaagc tacaaagaga
    11761 tctgcaagca gatggtatcc aatcagtatt ctctgatccg catttcttag aaatcacgcc
    11821 tacaggtatc actaaagctc attcacttgt ttttctggcg gattaccttg gaatcgaatc
    11881 gagtgacatc atggcttttg gcgatcaaga aaacgacatt gctatgttga attacagcgg
    11941 gttgagtatt gcgatgggga atgcacagcg acaagtaaaa gaaattgcaa accaagtaac
    12001 actgacgaat gaagaagctg gtgttgctta tattttaaaa aacttctatt aaaaagaata
    12061 aaatgacgat atgaatgtag gaggaaagaa aatgttagtt attattgata tgcaaaatca
    12121 tattcttgat ccaaacagcg aattttatat tccagagtca gatcaattgg cagaacgtat
    12181 tgctcaaaga ttagctaaag cacgccaaaa taatgaatat attctcttca cacgggatat
    12241 accagttgaa ctgaaggaca aagaagaaga acgagaagat ttgcaattga tccctcttct
    12301 ttctccacaa gaaaatgaac gagtgattaa aaagtattac tttacgattc cacctgaaac
    12361 tttagctgaa atccaggaaa ctttatttga aagcaaagaa gaacaaaaag agatcgaagt
    12421 agtcgggatt gaaacaaact tgtgtgtact ctctaatctg ataggcctgc agagtgcttt
    12481 tcctgaagca gacttttttg ttgatccaat gttagtctcc agtagaaaac acggagaatc
    12541 cgctttagag ctgttgaaag atttcaacgt ctctatcgtc gaatcataaa aatatctatc
    12601 ttcatttacg atatttaaca cacaataaaa aaagcacgca gaactgtttt agtcctacgt
    12661 gctttttcta tcaaagtaga ttggcttttt tatgacacag agtgaaacgg caggctcacg
    12721 acctctttct taaatggtgt tcaatcagct gaccctcggt attagcttaa caaacggcaa
    12781 aatatgagga actatttttc cgtttgttaa gctaatactc aggtcaaaac gctgatttca
    12841 caacctcttc cttaaacggt attcaaaagc ttgcatctgc agtaataaga acagccaagt
    12901 aaaaacataa gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt
    12961 tcacaacctc ttatgtacct tgtaggactc gaacctacga ccggacggtt atgagccgtc
    13021 tgctctaacc aactgagcta aaggtacatc ataaataaaa tttatttttg ttacttttca
    13081 actatagcac gaatgacaaa ctctcacaag tttcaaaata taagtaaaat aatattgtta
    13141 ttcttatttc ttttacttgt gtcataacta ttttgttact ttaattttta tttgcttatt
    13201 ttagaaaaaa tgttattctt ttatttattc tcaatgttag gagtgattat tttgccattt
    13261 attgcgattt tattcgattt agttgcggca gcagcttatt ttctccaatt aaattatcaa
    13321 acctcaggaa ttctgtttat tggaatgatt tttcaaggtg tgattaccct tttgcttctt
    13381 gtcatgatgc tcagctataa aggaaaaaga tatgcccgta ttcaaacaaa agtatttgtt
    13441 aaatatgttt ccattcgcta tgggatcatc attctttcct ttttagtgaa tgcgatcgtc
    13501 ctctttttat atgtattgaa ttacttagat atcaatccat tgattttttc tcgatagata
    13561 agacacattc agcggaacag ttcctgctta tcagcagaaa actgttctgc tttttcattt
    13621 ttcattgatc tattcttctt ttattttcct agcatttcat ttgtgatgta tgcaataaca
    13681 tgttatcttt tattatatga atcgttgagg agtccgttac atgattacgt tggtgagagt
    13741 tttgttttgg gtgcctgcgg tcgcactggt cgcaagtatt gtttatctta tgaattggaa
    13801 caaagaacgg ttttatttag ctattttgac tcttccggct atctacttta tgtggaaagt
    13861 ttttaattac aactactttg aacccgatag tgtttttata gaagaattat caggactggt
    13921 tttgtcttta ttgatcgtca ttctttattt gatacgttta aataaaaaac attgagtact
    13981 attgattgca tgtgtagctt tctatagtca ttataactat tctactataa tgaaag
//
LOCUS       DASJST010000068        13990 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJST010000068 DASJST010000000
VERSION     DASJST010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.068"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            555..2366
                     /locus_tag="U4B05_001666"
     CDS             555..2366
                     /locus_tag="U4B05_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050807.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEARPTDSMKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTLETPENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2427..3887
                     /locus_tag="U4B05_001667"
     CDS             2427..3887
                     /locus_tag="U4B05_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN2050808.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3898..6102
                     /gene="topB"
                     /locus_tag="U4B05_001668"
     CDS             3898..6102
                     /gene="topB"
                     /locus_tag="U4B05_001668"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2050809.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6118..6267
                     /locus_tag="U4B05_001669"
     CDS             6118..6267
                     /locus_tag="U4B05_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050810.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6342..6935
                     /locus_tag="U4B05_001670"
     CDS             6342..6935
                     /locus_tag="U4B05_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN2050811.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6948..7847
                     /locus_tag="U4B05_001671"
     CDS             6948..7847
                     /locus_tag="U4B05_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN2050812.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7850..8347
                     /gene="ssb"
                     /locus_tag="U4B05_001672"
     CDS             7850..8347
                     /gene="ssb"
                     /locus_tag="U4B05_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2050813.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8713..8973
                     /locus_tag="U4B05_001673"
     CDS             8713..8973
                     /locus_tag="U4B05_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050814.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9418..9624
                     /locus_tag="U4B05_001674"
     CDS             9418..9624
                     /locus_tag="U4B05_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN2050815.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9672..9875
                     /locus_tag="U4B05_001675"
     CDS             9672..9875
                     /locus_tag="U4B05_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050816.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9891..10328
                     /locus_tag="U4B05_001676"
     CDS             9891..10328
                     /locus_tag="U4B05_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050817.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10321..11028
                     /locus_tag="U4B05_001677"
     CDS             10321..11028
                     /locus_tag="U4B05_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050818.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11408..11587
                     /locus_tag="U4B05_001678"
     CDS             11408..11587
                     /locus_tag="U4B05_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050819.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11912..12175
                     /locus_tag="U4B05_001679"
     CDS             11912..12175
                     /locus_tag="U4B05_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN2050820.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12169..12519
                     /locus_tag="U4B05_001680"
     CDS             12169..12519
                     /locus_tag="U4B05_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2050821.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12543..13157)
                     /locus_tag="U4B05_001681"
     CDS             complement(12543..13157)
                     /locus_tag="U4B05_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2050822.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13471..13893
                     /locus_tag="U4B05_001682"
     CDS             13471..13893
                     /locus_tag="U4B05_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050823.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT         5171 a         2289 c         2844 g         3686 t
ORIGIN      
        1 aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa ctagtacgat
       61 tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa atgacgaacg
      121 gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa gaacgaatcg
      181 aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac gtggaaaagc
      241 ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga ctggatatga
      301 cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa ggtgaatcgg
      361 ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta gaacagctga
      421 aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg atcattgaca
      481 gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa ggctatcgaa
      541 aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa taccaagcca
      601 cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga ccaactgatt
      661 ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa aatgatccag
      721 aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc gatcaactca
      781 aaacattgga taaccaatac gccgcagaag caggttatta taaattctat tttgaggaag
      841 tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc gtcaacagta
      901 atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag aaggaacaaa
      961 aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa gaaacaaaaa
     1021 caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa gttaaatccc
     1081 tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa gacgtcaaag
     1141 aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa ttattgattg
     1201 aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa aaagaaggaa
     1261 tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg gtcaaagggt
     1321 tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa gataaaaaac
     1381 gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt gaaaaagtgt
     1441 ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt ccgaaccgtg
     1501 tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg attcaagcgg
     1561 tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc tatcaaagag
     1621 aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa gaagaaatcg
     1681 tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat gaattagccc
     1741 atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg caggcagaaa
     1801 tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa gctattcctt
     1861 atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct gcggaacgtg
     1921 ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc atttctaatg
     1981 aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg aagcaggagc
     2041 ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt tcactttgga
     2101 tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta catttgaagg
     2161 atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga aaaagttatg
     2221 gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat ccattccgat
     2281 tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat tatgaacaac
     2341 aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa aaacggatag
     2401 tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt attgatcgat
     2461 gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta gtcaataaag
     2521 gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga aaacaacgtt
     2581 tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa ctttttttca
     2641 tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat tttattctaa
     2701 agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa aagtacaaag
     2761 atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg aaggaagaac
     2821 gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt gccgaactaa
     2881 agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat catgcggtcg
     2941 cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat caaggaacgt
     3001 tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat aaaaattcga
     3061 cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa ttttttgaga
     3121 gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac gatacgtggt
     3181 tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt gaggcagtat
     3241 ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg gataatgacc
     3301 gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat ccatttacga
     3361 atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca aaagaataca
     3421 aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct attatggcca
     3481 tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct cacaaagtaa
     3541 atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt aatttgaaag
     3601 atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat ggtacgtacg
     3661 attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt ttctcttata
     3721 aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta cctgaagtaa
     3781 aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc agaaagcaaa
     3841 aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat cgtcatgatg
     3901 aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga tagcttctct
     3961 aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc tggtgaaact
     4021 gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat gtacgatgaa
     4081 aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta tcactatcat
     4141 gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca atcggccgac
     4201 accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg gacgatcatt
     4261 caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa ctccttagaa
     4321 aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac ctatccaaaa
     4381 tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa cggtagccca
     4441 ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt aggcagagtc
     4501 caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa tttcaagaaa
     4561 gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt tgatgcgaag
     4621 cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt aaaagaaaag
     4681 ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga gaaaaaaagc
     4741 gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa tcagctcatg
     4801 aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg gaaatatcta
     4861 agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta tctgttggat
     4921 cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc tattcataca
     4981 ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat cattcctaca
     5041 aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca agcgatttac
     5101 gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta tgaagaaaca
     5161 acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt accgatagat
     5221 ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga agaagagccg
     5281 ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt ccttgaaaaa
     5341 gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat gaaaacagct
     5401 gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga aggaatcggt
     5461 acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata tatcaaggta
     5521 gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc tgtggaaaaa
     5581 gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt gttaaagatt
     5641 ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt tgtctctcac
     5701 ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac gttacaagaa
     5761 gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg tccaaaatgc
     5821 caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga agcctgtgat
     5881 tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct gaaggagatt
     5941 attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa gggttcgttt
     6001 gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc tgaaaagaaa
     6061 aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt taatcttatg
     6121 cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat tcgttcagaa
     6181 caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca gcaacggatt
     6241 caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca ctatcatgat
     6301 atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag aaactgaaag
     6361 aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa ttaggaatcg
     6421 cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt gatcgtggtt
     6481 attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa cgtttttcca
     6541 ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg aatatgccga
     6601 aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt acaaaagagc
     6661 agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa gtagagggaa
     6721 cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt tgtgatattt
     6781 ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa gagtacatgg
     6841 aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta catgtagaaa
     6901 aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg acaccgacac
     6961 aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca cagttagtac
     7021 aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag tataaaaatg
     7081 atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat agaaggacga
     7141 caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa ttgaagagta
     7201 tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct attcaaggaa
     7261 tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg gtggctaatt
     7321 tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct atttatcctg
     7381 aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag attccagctt
     7441 atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt ggcacagata
     7501 attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa aatatcgata
     7561 cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat acaaaaccgt
     7621 tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat ttggaaaagc
     7681 agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt acagatacag
     7741 tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa gaaagaaaac
     7801 ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga tgatcaataa
     7861 tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg cgagtggtac
     7921 tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa acggcaatcg
     7981 agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa tggtgacatt
     8041 agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttatgacaa
     8101 ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc aactattaga
     8161 aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac
     8221 caccactttt gaacaacggg atacagcgac gcctaataac aatggattga acgctttaca
     8281 gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg atgatctacc
     8341 gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata atttagtcaa
     8401 caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt ggttttcttt
     8461 tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca aatttacaat
     8521 caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa acaaaatcaa
     8581 aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg tagcagacag
     8641 caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta taattttagc
     8701 gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt aataaaaacg
     8761 caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa ttgtgtagtt
     8821 gcagatattg aaattatcga taaattgcta attatggatg atggaaaata caatattgcc
     8881 gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta tgttagaaat
     8941 cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa tcctaatgta
     9001 ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc tttttaggaa
     9061 ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc gccgttcgag
     9121 aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa gcaagacaaa
     9181 atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct ttattgattt
     9241 gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa gggtaaatgg
     9301 cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca acaaatcaat
     9361 aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga atcaatcatg
     9421 cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa ctatcatgca
     9481 ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag ctatcgtttg
     9541 tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta tattttgatt
     9601 gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact gtaaaagaag
     9661 tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa gctttactta
     9721 ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact atgctatttg
     9781 aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg gaaacttatg
     9841 aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat atggctcaaa
     9901 attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa atggaggata
     9961 tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa agattgtcgg
    10021 tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg acgtactatc
    10081 gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc ttactatgct
    10141 tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct ttagaactaa
    10201 cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa tggttagatt
    10261 attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag gacaacgaaa
    10321 atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt aacaaacaaa
    10381 gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg
    10441 aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac gagtcaaacg
    10501 aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga aacggtaaaa
    10561 aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt ttacctagaa
    10621 aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca ctattactca
    10681 gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag
    10741 ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag atttttgacg
    10801 tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt acccgaagca
    10861 tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta
    10921 actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa tgaacttgac
    10981 cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc atttataccg
    11041 aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat ggttcttatt
    11101 tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag cgggcaaagc
    11161 ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc tgttgttcgt
    11221 actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa acttcgtagt
    11281 attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag cccttgtgag
    11341 tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat gaaaggagag
    11401 agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag atgaaaagag
    11461 acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta agattatgtg
    11521 tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac cagcactaaa
    11581 aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt gttcctacta
    11641 tttagaatat tttctatgtc ctttttattc cattttgtat caataactat gaaatcttta
    11701 aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc ttaaacatat
    11761 tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga ttcatggaat
    11821 gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt atgtatgtta
    11881 tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa aaaaatgggg
    11941 aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa ttcatacaga
    12001 agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa ttatcgaagc
    12061 ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca ctgatgaaag
    12121 aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat ggtgagaaaa
    12181 cctagacaag gcgatattct attattgaat acagcacctc gatctggaca cgaacagaca
    12241 ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag taacgttgtt
    12301 acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa tataaactca
    12361 gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac aattgattat
    12421 gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga ggagttattg
    12481 atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag cagcatgatc
    12541 aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg tctcgtaaca
    12601 ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc aacgatgttt
    12661 ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt aggacgtcct
    12721 ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg tttagattcg
    12781 gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt aatgaattta
    12841 tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat gatcagctgc
    12901 acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt tcttccaagt
    12961 cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag tgcttcttga
    13021 aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat tctctctgca
    13081 ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga tgagacacgt
    13141 atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa acaccctaat
    13201 catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg gtatacctta
    13261 tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt taaatggcaa
    13321 tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat taaaaatata
    13381 ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta agaaaacgtt
    13441 ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt atctttaggt
    13501 attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc tgatagtaca
    13561 aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga taggaacgga
    13621 atagccattg gtaacccaac taacgactta actgttactg aaaattattt aatagtttct
    13681 ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat gatttttgat
    13741 aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt
    13801 tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa caattggatt
    13861 gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga atttgtgaac
    13921 acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa
    13981 tcagctttac
//
LOCUS       DASJST010000069        13976 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJST010000069 DASJST010000000
VERSION     DASJST010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.069"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..663)
                     /locus_tag="U4B05_001683"
     CDS             complement(52..663)
                     /locus_tag="U4B05_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2050824.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(786..911)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(993..1709)
                     /locus_tag="U4B05_001684"
     CDS             complement(993..1709)
                     /locus_tag="U4B05_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2050825.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1715..2284)
                     /gene="scpB"
                     /locus_tag="U4B05_001685"
     CDS             complement(1715..2284)
                     /gene="scpB"
                     /locus_tag="U4B05_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN2050826.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2268..3065)
                     /locus_tag="U4B05_001686"
     CDS             complement(2268..3065)
                     /locus_tag="U4B05_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN2050827.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3035..3406)
                     /locus_tag="U4B05_001687"
     CDS             complement(3035..3406)
                     /locus_tag="U4B05_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2050828.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3425..4312)
                     /gene="xerD"
                     /locus_tag="U4B05_001688"
     CDS             complement(3425..4312)
                     /gene="xerD"
                     /locus_tag="U4B05_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN2050829.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4408..4881)
                     /locus_tag="U4B05_001689"
     CDS             complement(4408..4881)
                     /locus_tag="U4B05_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2050830.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4986..5849)
                     /locus_tag="U4B05_001690"
     CDS             complement(4986..5849)
                     /locus_tag="U4B05_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2050831.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6045..8144)
                     /locus_tag="U4B05_001691"
     CDS             complement(6045..8144)
                     /locus_tag="U4B05_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2050832.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8364..9473)
                     /gene="rpoD"
                     /locus_tag="U4B05_001692"
     CDS             complement(8364..9473)
                     /gene="rpoD"
                     /locus_tag="U4B05_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN2050833.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9497..11338)
                     /locus_tag="U4B05_001693"
     CDS             complement(9497..11338)
                     /locus_tag="U4B05_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN2050834.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11520..13835)
                     /locus_tag="U4B05_001694"
     CDS             complement(11520..13835)
                     /locus_tag="U4B05_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2050835.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
BASE COUNT         3679 a         3016 c         2407 g         4874 t
ORIGIN      
        1 gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac
       61 tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc
      121 atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat
      181 cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa
      241 aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag
      301 agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac
      361 actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc
      421 tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa
      481 cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc
      541 actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa
      601 aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt
      661 catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa
      721 tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa
      781 ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc
      841 tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc
      901 tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa
      961 cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg
     1021 acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct
     1081 ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc
     1141 acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga
     1201 tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct
     1261 tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact
     1321 ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt
     1381 agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc
     1441 atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact
     1501 cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc
     1561 acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt
     1621 acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg
     1681 tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa
     1741 agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca
     1801 gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata
     1861 tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac
     1921 gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg
     1981 gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg
     2041 tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat
     2101 ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta
     2161 attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt
     2221 cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg
     2281 tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt
     2341 tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact
     2401 tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt
     2461 atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt
     2521 ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa
     2581 tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg
     2641 ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca
     2701 gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct
     2761 tcttcccata attcttcatc gtctgtgacg aattcttgga caggaagcaa tgtcttgctt
     2821 ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt
     2881 gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca
     2941 tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat
     3001 acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta
     3061 gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact
     3121 tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca
     3181 aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt
     3241 gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt
     3301 acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata
     3361 gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct
     3421 atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat
     3481 gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca
     3541 aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta
     3601 cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc
     3661 gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa
     3721 gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag
     3781 gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa
     3841 gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt
     3901 ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct
     3961 tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga
     4021 tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc
     4081 tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct
     4141 ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct
     4201 gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca
     4261 atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc
     4321 ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc
     4381 tgagcaaaga aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata
     4441 gcattcctga cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg
     4501 ttcttctact acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc
     4561 gcagttcaag catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata
     4621 acgggctaat ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc
     4681 aagggttcga taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa
     4741 gatttcttct gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc
     4801 tctttgcggt gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc
     4861 cgaaatagca ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa
     4921 aaagatataa cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg
     4981 gattctcaat tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct
     5041 tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc
     5101 atgatttctt ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct
     5161 aaaatggtta aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat
     5221 gaaagattca gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct
     5281 agacgaggtt cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt
     5341 aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc
     5401 atttcttctg ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc
     5461 caaatacggt cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc
     5521 catagctctg tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga
     5581 ccgatatcga caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaagcgtaa
     5641 tgaccgatac ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg
     5701 taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca
     5761 aaggtttgtc cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct
     5821 gtaaatatag ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct
     5881 taatacattt ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt
     5941 tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat actccccttt ttcgaataaa
     6001 cggtgttaaa cggcaggctc acagcctctt tcatttgcct aaaattaatt gctcccattt
     6061 cctccgaacc atccagtttt acagttgatg gttacgatgt aaaatggcaa agcactttct
     6121 ccaatgcctt tcatcaatgc agattttgtt gaagtggcat atagattata atagccttcc
     6181 ccattttcga ttctggcttt ttctaatctg taacctcctt caacgatata tccacgttgg
     6241 atacatgtag caatcacttt gctttggaca cctggtgccc acgtccatgc tggattagat
     6301 gcatcggcaa tatcacttgc tctggcagat ttgattggct gattcgctga gtttgaagag
     6361 gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg
     6421 gcttgagctg cagctgctgc ttgagcggct tgttttgctt gttcttcagc tgcttttttc
     6481 gcttcttctg cttttttctt ttcctcttct tcttttttct ctttatcagt gagcatctta
     6541 tctacttgtt tcaattgttc attcaactga gcagatagat ctgtattttt cactttttct
     6601 actgcttctt ttgccgtctt gtattggtcg cgtgttgctg aatccactac ttttccgtct
     6661 ttaaagacta tagctgtttt ttctttagct gtttgcaatt cagcatattg atttttagct
     6721 tctgtttgtg cttgttgaat caattttcca aaatctgtgt tatccgtttc gattgcttgg
     6781 atctctttat ctgctttcaa cttgatgtca tctgctagat gatcatcgac gatggcaggt
     6841 gctatgaacc actcattcac tttttggata tctcggatct tagattggat atcttggtaa
     6901 gttgtttcta actctgaata tcctttttca ttcttaagac tggataaatc attttttagt
     6961 tttgataaat cttgatgaat catatctgca cgaataaatt gatggtgatc atctgtataa
     7021 aaggcagcaa gtttctcatg aagttcttcc atttgttgct gtctttttgc ttctgctgct
     7081 acttcggctt ctactttttg atgatggtca taataagccc agcttcctcc accgagtaga
     7141 agtactgctg ctaaaacgag ataaggtact ttttttgatt ttttcttttg cgctttatct
     7201 ggatctgttt tttctaagtt tgattcttga tgtgtttttt cagtatctgg cacgattgtt
     7261 attgatttcg cttctgcttc aaacagctta ggctggttct cttctttttc tatttttgct
     7321 tcaagatcag taccctcttt gcttacagat tgattttcag catcggattc actatttatt
     7381 tcatcgggct ctgttgactt tttgtctgtg ttttcgagta tttctttttt ctctgcttcc
     7441 ggcagtttat tcgttttttc cttttctaat tgtttttcag taaatacttc agttttttct
     7501 gtgatatccg tttttacgtc ttttattttt actgtttctt tacctgtggc gttttctcta
     7561 tcagagtctt tttcagtttc ttgttccttt tctggctcta ctttgttttc ctgtgcaatt
     7621 ttttcactga tcattttgag cagctctttc gcgtgctctt cttttgtatc ttcacgatgt
     7681 gctttgatat aagcggatag tatcgaattt tcttcctcat tatcgacgat ttctttcagt
     7741 tcagctactc ttcttttttg ttttgattca agcgtttctt cttgattctt tcctgattct
     7801 tccgaagtct catgaacagt tgctttcttc tgttcatttt tctctatatt agttacttga
     7861 tcgtgagcag gtggttcttc gtcttctggt tctgcttttt cgaataattc cattacagat
     7921 tctagaggta aatctttcaa ttccgaccat tcgattggat cgtttaattc tgtatcagga
     7981 aagactgcat cttcagttga tttatcctgc atagctgcat ctgtatttgt ttcttcaatt
     8041 gattctttta tttgctgatc ttctgcagtt atttgatgac cgcagtctgg acagatagta
     8101 ttctgctcca tttttttcga tccgcaattt ggacattttt tcatcactta gcgatcggct
     8161 cctttactag gtttacgcta ccatttaatc tatcatattc tatacgtcac gccaatagct
     8221 tatttttagg atttacttaa aaataatgaa tgaatttcct tcctatagaa aaaaaagctt
     8281 gagactgcgt agtctcaagc tttttttctt ttaaaataaa ccaaacagtt ttactgattt
     8341 ctcttctggt caatagtcca cgcttattct aagaaatctt ttaattgttt tgaacgagaa
     8401 ggatggcgca attttcttaa tgctttagcc tcgatctgac gaatacgttc ccgagtaaca
     8461 ccaaatactt tccctacttc ttccaatgtt ctcgtccggc catcatctaa accaaaacgt
     8521 aagcgaagga cattctcttc ccgatcagtc aatgtatcta gtacatcttc caattgttct
     8581 tttagtaact cataagctgc gtgttctgct ggacttgttg cttcctggtc ttctatgaaa
     8641 tcacctaagt gagagtcatc ttcttcaccg attggcgttt caagggaaac aggttcttgc
     8701 gcgattttca agatttctcg gactttttct gttggaagat ccatttccgc accgatttct
     8761 tctggcgtag gttctcttcc aaggtcttgc agcaattgac gttggatacg tatcaattta
     8821 ttaattgttt caaccatatg aactggaata cggatcgtac gtgcttggtc agcgattgct
     8881 cgagtgatgg cttgacgaat ccaccatgta gcataggttg aaaacttaaa tcctttgcgg
     8941 tagtcaaatt tttcaactgc cttcatcagt cccatgtttc cttcttggat caagtcaagg
     9001 aactgcatgc ctcgtcctac ataacgttta gcgatactta ctactaaacg aaggtttgct
     9061 tctgccagac gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc taacgctact
     9121 tcttcttccg cagttaaaag ggagacacgg ccaatttctt tcagatacat acgaaccggg
     9181 tcattgattt ttacaccggt tggcgcagaa agatcttctg tttttgcttg ttcagctttt
     9241 ttctcagcac tcttcaagct gtgaattgat ggatcaccat tttcatccac cacactgata
     9301 cctgcgtctt caatcttttg gatcaatttg tccatttcat ctgcattcaa agtgaatggt
     9361 gtagcaagct gattggataa atcatcatat acaacttgtc gtttgggttt gttttctttg
     9421 atgaaagctg ctactgctgc ttcatacgtt tttttatctg ttccatttgc cataaaagaa
     9481 ggcccccctt gagtagctat ttggcttgtt ttaactgttt cgttaaattg atgatttcaa
     9541 tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt
     9601 tttttaatgt tatttcatca gctaagccag atttgctgat cacatctagc aagtcttgga
     9661 tttctcgtgg cgaactttct tctgccaccg gtaaatattc gatttccacc accaaccgcc
     9721 ttaaattttc atcttgcaaa aagtctaaaa actctgcaag gttgaattca taatgttgtg
     9781 agacatatgc atcgaataaa aaataaactt ctgcatatgc atcatgaata aactgaaatt
     9841 ctcgttcttt taaaatatta cggacagaaa cttctttaaa tgcacgatat aatagaatct
     9901 gctctgcctt ttctacttgc gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa
     9961 cctgagtgtg ttgactttcc tgaggttctt gacgattttg ctgatttgtc cttcgaagat
    10021 cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa ctctgttgac agttgagata
    10081 gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt aattcttcga
    10141 ggtattcgat cttgtctttt tcattatcta aatttttccc ttgttttaaa taacgtgatt
    10201 taaaagtaaa gactgtttcg cgcccatgag ccactaattc ttggaaagat gcttctccat
    10261 acttacggag atattcatca ggatcaagct tttctggaac gactacgaca gaaagtgcta
    10321 atcgactgtt ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt
    10381 ctccgtcata gcaaaagacc aattctttcg tcagcttttc catttgttgg atctgttgag
    10441 ctgttagact tgtccccata gaagctaatc cagttttgat accagcctgc catgcagcaa
    10501 tcacatccat aaacccttcg aacaacaaaa tttctccgtt tttccggata tcgcttcttg
    10561 ctttatcaag attaaataaa acttgtcttt tattgaacag ttctgtttct ggactattca
    10621 agtatttggg ctgatctttt tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac
    10681 cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac tctttgataa aaacgatcag
    10741 acatacggcc atcatcatgc gtgacaaaca atccagagcg ttctaacaat tcatttgaaa
    10801 ttgattcatt cttgaatact tgctgaagaa aagaacgctc attaggtgca aaacctatgt
    10861 tgaattcttc aattaattct aaagacaacc cacgactttg caaatattct aaagctggct
    10921 gtccagcttt tgtatgcaat agcatgtgat gatagatttc cgctgctttt tcatgagcag
    10981 caatcagttt ccccgtagca gattcaggat tttggacagc tatagtacgc tgacgccatt
    11041 gatcttctaa cggtatctgt tcgaagtctg ccacttttac cactgcttct ggaaaactta
    11101 gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat
    11161 gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt acgttcttca tgaaaaggac
    11221 acagccccaa gtaattttta ccagactttt tcaactggac atattgtcca atcacctcaa
    11281 cgatattcgt ttgactgcgt actgtttcga taacctcttg aggaatccgt tgagccattt
    11341 tttcaccccc tgctctattg atatcttttg ggtttaacag aagatatttc aggagaataa
    11401 ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta aaggacacaa ttatacatgt
    11461 ttgattgtat catatttcat tgctttttca agtataacac atttctatct cattaagcat
    11521 caatttgcta ctcttttcag taactgattt tgaatatcag ccttccagta caatactcga
    11581 agaacaatat ttacgagcat gaatacaaag aagtatccta cgctctctcc caaccctaag
    11641 tttacttcca aaattccatg gaacagcatt gcagctacat atatccctac gatcgtcgtt
    11701 actgctagga agatgcgcaa tgggttgaag ggataacaag cttttacaac tgccatgcaa
    11761 ctgatcccaa tcagcaagta atacatcaaa gtagtagttt cagcatttcc ccatgcgaat
    11821 gtttttccaa gtagatacac cgcaatgata ttcactacta caagcaaggc atttggtaaa
    11881 gcattaataa gtgctgtagg caaataacgg taagtgattt ttcttttatc ttgttcaaaa
    11941 gataaaaaga aggaaggata cccttcaatc gctaaatcaa tcagcgtgat ttggataggg
    12001 ataaacggaa atccaattgc tgtaacggca catataatcg ataaaataaa tgaataaatc
    12061 gttttgataa aaaagatgcc tgagactttc gtcacattgt tgaccactcg gcgtccttca
    12121 aacaggactt ctggcaatgt agtaaaatca gagtccaaca ataccagatt cgcaatctga
    12181 cgtgttgctc catcaccttc tgccatagca atgctgcaat ctgcttctct taacgccaaa
    12241 acatcattca caccgtcgcc tgtcattgca accgttctgc cgctttcttt caattcattt
    12301 accagcaatt tcttttgctg cggactcact cgtccaaaaa ccgtatagcg atgagcagct
    12361 tctcgcactt ccatttcttc agtaatttga gaaagatcga catacgcatc ataattcggt
    12421 aatcctgcac gtctagcgat attggagaca gtaaaaggat tgtctccaga aatcactttc
    12481 agatccacac cttcctcatg aagataagct agtgtttctt gagcgttttt tcggattgga
    12541 tcatcgattt caaacagagc aaccggctca acaaatgctg gtttttcttc gttcaattgc
    12601 tgatttggag cgatccccaa cataagtacg cgatagccat tcgcttgggc tgtttttaga
    12661 ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta cacgactccc
    12721 agttctggaa attccatcgc gccccatttg cgatcagaag aaaatgcgac tttgtttgtc
    12781 acatcatacc gattagaaag ctcaaaataa tctctcatag cccgcatagt gatgttgtta
    12841 tcactgcttt ctgccagata cgtccccata atttctggga tggctttctc gtacagcgga
    12901 tgtagtgctt ctacccgctg gatactcatt ttcccttcag taatggtccc tgttttatcc
    12961 aagcataatg tatcgacatg ggccagcgtt tcaattgaat acatgtcttg aacaaggatt
    13021 cgtttttttg ccagtttggt tacccctgtc gttaatgcaa tacttattag caataccaaa
    13081 ccctttggaa gcatgcctaa caaagctgct gcagatgcaa taacagaaat tttgatagaa
    13141 gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa
    13201 ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt cagaacgaat agctttgtga
    13261 gtctttgcct cgctagtcaa gcggacagca taattatctg cacctactcg gataacttca
    13321 gccatgacag aaccgcttgt caaaaagctt ccagaaagca attcagctcc agtttctttg
    13381 acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg tccagatata
    13441 acctgtaaat cggctggtat ctgttctcct gctccgattt ttaccaaatc tcctaatacc
    13501 agctctttag tagaaatcga ttgttcttta ccgtcccgga tcactagtac ttctgattgg
    13561 gaaacaatcg ataatttccg tacaagattt cttgcatgta tttcttgata aattccaata
    13621 acgatattga cgacaataat tgctaaatac gccatgttgg aatacgcacc tacaaataac
    13681 agacaaatcc caataccaaa gttcagaaaa ttgaataacg tcaatatatt atcgctgaat
    13741 atttgttttg tactttttga tgcatcttcc tggtagtcat tttgctggcc agaagccatt
    13801 ttctgaacta cctcttgctg tgtaagtcca ttcattttct ttttctccat tttccgccaa
    13861 ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca agattaaaaa caacttgttt
    13921 ttataaaatt tatagaaaaa cacacgaatg attttacaaa aaagatgcca accttt
//
LOCUS       DASJST010000070        13890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJST010000070 DASJST010000000
VERSION     DASJST010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.070"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(19..843)
                     /locus_tag="U4B05_001695"
     CDS             complement(19..843)
                     /locus_tag="U4B05_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2050836.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(816..1496)
                     /locus_tag="U4B05_001696"
     CDS             complement(816..1496)
                     /locus_tag="U4B05_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2050837.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1519..2457)
                     /locus_tag="U4B05_001697"
     CDS             complement(1519..2457)
                     /locus_tag="U4B05_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2050838.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2702..3565)
                     /locus_tag="U4B05_001698"
     CDS             complement(2702..3565)
                     /locus_tag="U4B05_001698"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN2050839.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3707..6280)
                     /gene="mprF"
                     /locus_tag="U4B05_001699"
     CDS             complement(3707..6280)
                     /gene="mprF"
                     /locus_tag="U4B05_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2050840.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHLTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6421..7227
                     /gene="proC"
                     /locus_tag="U4B05_001700"
     CDS             6421..7227
                     /gene="proC"
                     /locus_tag="U4B05_001700"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN2050841.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7306..8862)
                     /gene="rny"
                     /locus_tag="U4B05_001701"
     CDS             complement(7306..8862)
                     /gene="rny"
                     /locus_tag="U4B05_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN2050842.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9273..10322)
                     /gene="recA"
                     /locus_tag="U4B05_001702"
     CDS             complement(9273..10322)
                     /gene="recA"
                     /locus_tag="U4B05_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN2050843.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10431..11672)
                     /locus_tag="U4B05_001703"
     CDS             complement(10431..11672)
                     /locus_tag="U4B05_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN2050844.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11912..12490)
                     /gene="pgsA"
                     /locus_tag="U4B05_001704"
     CDS             complement(11912..12490)
                     /gene="pgsA"
                     /locus_tag="U4B05_001704"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN2050845.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12699..13622)
                     /locus_tag="U4B05_001705"
     CDS             complement(12699..13622)
                     /locus_tag="U4B05_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050846.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         3969 a         3099 c         2429 g         4393 t
ORIGIN      
        1 gcttcttttc tttataattt agttattttt tcttctgaag gcaaccaata ttcgtttgat
       61 gatatttaca atcaggaaca aagcaataaa gactaatgtg attgttgctc ctggaggtgt
      121 atctagataa aacgagctag ttagtcctga cagcatccct agaaatccta cacaaacact
      181 gatagcaatc acgacattga agcttttgcc taatcgcata ccgattgctg cgggaagaac
      241 catgattgcg gatatcaaaa gtgcaccggc aatcgggatc atcacagcaa tagccacacc
      301 agtaatcaca ttgaacatca tcgacatcaa atgaacgggt agcccatcca cgtgggcggt
      361 atcttcatca aaagtcagaa catacatcgg ccgtttgaat aaaaagaaca atataccgat
      421 ggcaacgaat aagattccta aaaagatgac ttgctcccat gtaatggtca cgatcgaacc
      481 aaacagatag gattggatac ttgttgctga attccctccg ctcaaattca tcaatactaa
      541 cgctagcgcc aaaccaccag ccattaaaat ggcaatggag atttctgaat aggttcggta
      601 gatcgttcgc agatactcca atatcacagc tgctaataca acgactagca tggtcgtaag
      661 tgtaggatta aggttcaaaa agaatcctaa cgctacccct gctaaagaga catgagaaag
      721 cgtatcagcc atcagcgatt gtcttctgat cactaaaaac acacctagca tcggggcgat
      781 tccggcaatg aagatagcgg caagaaacgc tcttctcatg aattcatatg aaagcaacgc
      841 cattgagaat cctcctttct caccagtcgg atctgtctgt ccgcatactg tttgatatct
      901 tcatgatcat gggtgatcat tagaactgat ttcccatgga catgcgcact atgtcggaga
      961 agacgataaa attcatttct ggattcttca tccatacctg ttgttggttc atcaagaaca
     1021 aataagtcag ggtcagtagc aaaaatacgc gctaaactga ttcgctgctt ttgtccgcca
     1081 gacaattcac ctattcgccg atttcgcata tcccacatcc ctacagcttc taaagcacgc
     1141 tgtacatgtt gatgatcttt ttcagagaga cgcttgaacc agcggcctct tggatatctt
     1201 cctgaacgga ctaattcgat caccgtacta ggaaaacctg cattgaaaga tgcaacttgt
     1261 tgaggaatat aaccgatact taattttttt ccttcttgat ttttctcagc aatctttatc
     1321 attccttttg aaggtttcag taatcctaac gatgctttca ccaaggttga cttggctgct
     1381 ccattttctc cagttaaaat gacaaattca cccgcatcta catgataaga gacatcttct
     1441 agtacaggtt catcatcata gtaaaaggtc aaatcagata tttcgatata acgcacctta
     1501 caacctccat tctaaaactt aattgatact ttcttttaaa gctttcagat ttttttccat
     1561 gacagaaata taattttcgc ctgctttgat ttgttcatta gtcaagcttt ctaaaggatt
     1621 caacacttct agcttcactc ctgtttcatt cgacaatgtc tcagctacct tcgaagaggc
     1681 attttcttca aaataaatca ctttgatgtt gtttttcttc acaaattctt ttagctcagc
     1741 taagcgactt ggtgtgggtt cctgatctgg tgaaacgccc gaaatcgcta cttgttttaa
     1801 gtcgtattga ttcgctaaat aagcaaaagc agcatgctga gtgacaaatg tgcggtttgt
     1861 tgcatctttt agctcatcgg agtatttttg atccagtgct tcaagctttt tgatataagc
     1921 atcggcattc ttcgtaaaag tttccttttt atctggatat ttttcgccta attcttcagc
     1981 aatcgttctg acttctttta tagctaatac cggatcgagc caaacatgtg gatcgtattc
     2041 atgtgaatga ccatgccctt cttctgattc ttcatgttcg tctgcttctt gcgattctaa
     2101 acggtcgatt cctttagctg cttcgatcat caaggtctgt ttgctatcca ctgaatcctt
     2161 taacgaatcc acaaaaaatt ccatgtctga actattatac acgaaaacat ctgcatcgga
     2221 aatttctgct ctttcttttg cacttggttc gaaatcatgc ggctccgttc cagcagggat
     2281 caaaagtttc acgtttcctt catcccctac gatctctttt gtaaaatcat acatcggata
     2341 aaaagtcgta acgatcgtca aatcctcttt tttgtctgct tccttgttcg tatttccaca
     2401 tgctgcaaac agtaaagcac ctatcattac cgtagctgtc aaaagatatt ttttcattta
     2461 tatgctcctt tcaatgaaca aatcggaatt ttacgattta ttaacgtgaa ctaatgtatc
     2521 aaaataaaaa aacacagtca atagaaatcc atcaatttct gatttaagca tgtatattct
     2581 tgattaaatc gtaaaatgga tgagttgctg ctcatatcta gccatttcac tacaataaaa
     2641 aactgaagaa taccatttta ggattcctca gtttttcatc acctatttta cttcttatat
     2701 tttattgcag ggttcgtact aaataaactt cctcacaaaa accttttaac ccattatata
     2761 ctctctgcgc tcttgaataa tgttgacaca aggcaaaaac ggttggtccg cttccactca
     2821 tcaatgcagc atctgctccg tacttgatca tacgctcttt gatttgatgg atgactggat
     2881 gccgctttcc cgttatatct tctaaactgt ttcccatttt tttgatcatc tgctgataat
     2941 cttgctctcg gatggcttca gcaagtccgt ggatgtcttg atgttggatg gcttgcgtat
     3001 ctacttctct aaacactgta cgtgtagaga cactcattct tggtttgacc aacacaaccc
     3061 agcactgtgg catagaaggt aatacttgga tttcttcccc tttgccaccg acaaatgccg
     3121 tacttccgta gacacagtaa ggaacatctg taccgacttc caaaccgatt tctgccagtt
     3181 cttctaacga acagcccaga ttccataatt ggttgatccc tcttagagca gctgcacagt
     3241 ctgtacttcc tccgcctaat ccagctgcta ctggaatatt cttcttaaca aagatcttga
     3301 caccggtttt gatattgtat ttatttttta gcaataaagc agcttgataa acattgttcc
     3361 gttcatctac cggtaaaaat gctttatttg tttctacaat gattttgtct tctgttattt
     3421 cttcaaaaaa tagatgatct gccaagtcta cgctcgacat caccatttcc aactcatgat
     3481 acccatcttg ccgtctatat aatacatcca gtcctaaatt tatcttggct ggcgcttttt
     3541 caatcaattc catcctctca cccattcgaa aagtctaagt cgcaagtatt ctatcatagg
     3601 gaaaaataaa agtatagctt atgtctgcct agtggttgat tttgtcatcg tttcaacagc
     3661 tttcacagaa ttttctcttc ataatccaaa caagttctag tgattcttat aattctgtga
     3721 gcacttcttc tgccaaactc ggaacatttc ctttttcttt tcctatcaga ctgagtagct
     3781 gcatggtagc aaatccgaaa ttacttcctt tcctataagc aaaataacgt ccttcccaat
     3841 gatcgacata ttttaatttc ttttcataag cagactggaa ggaatagaaa aaattaccat
     3901 aattatatac gatatgcatg actttttctt gaaagaaaga aaaatctgtt tctccaacat
     3961 tagctaatgg agctaagcct agattggctt ccgagtagcc cattttctcg tatacaataa
     4021 acagattggc taacagatag tcagctaatt cctcagattc tttaggtaag acccgcaaca
     4081 aatcatatcc cgcctgcttc ttcgtcatcg gctgagcggt gatgaagcca acgacttttt
     4141 gatctttacg gacaataccg acaccgctag ataatacata tgatttatca aatcgtccca
     4201 cagagaagta tttttctcgt gcaccttgaa gccatttttg tgaaacttca tttacagctt
     4261 gatgtaattc ttccggcagt tgatcgtacc aagtaaaatg gtatccttct ttcatcaaac
     4321 gttgatattc aagttgctga ctcagccgag acaagtcttc tgttgctaga tcaacaatcc
     4381 ctgcttcacc tactttcgtg aggtgaaaac ccaattcatg aagcatcact acgtagcttt
     4441 ctgatatctt ataaaacgca agctgatagc ctaaacaatc tgcttgtttc ataaactgat
     4501 tcgttgctgc ccgcatcatt ttaggattgc caactggatc tcctaaaaca aagcatttgt
     4561 ttcctttgat ctgataacca aaaacgactt gatcttggtc gtcttcttgg taataaaaat
     4621 aactgtaacc tgacaagcga agctgatggc ttgtagctgt gcctccgtat gtttccaata
     4681 aacggtcaaa acggtctttt tcgtactcac tgccaagctt cgtagtatct tcagctaaat
     4741 attgataaag cgccactaaa gctagcaatg agatgcccaa accaataagc cctgagaacc
     4801 acacatcatc tgaaggaaac aaaataaaat gatttcctgt tattcgaccg actaaaaagc
     4861 gtccgctgtg atagcctgca attgcgtaaa cgataaacaa aaagccgaac aagctagcat
     4921 caaagattat agctccccaa gaataaacga attttgttcg ataaaattct tttctggata
     4981 accacacagc taacagaatc aagacatata ccaatatcaa ctgccaagat aacgtacgtg
     5041 tgattgtatt taaaatacca aaagatagta aagcaatcgt aggcagaaat gcctttttga
     5101 cttttactga aagtgctcta gcaaggccga gtaataaaaa gccaaccaat agattcactg
     5161 tctgatctaa aaaattgaat gagaacggca ataatacttg aaatagccga ctgattgcag
     5221 acagattagt gattgttgat agaagtacca tcatgatccc cgcaaaatag acagcactta
     5281 cgagcacgat atgcgcaacc ttttggaaaa acagtcgcgg aatgttatct aaaaatcgat
     5341 tgattttgac tcctgtatgt gtgatgaaca atagaagtcc cgtcataaaa ggtagaacat
     5401 aataaaacaa ccgataatat aatagccaaa ctactgctac atcttgccga acaggcaatt
     5461 gactcattcc taagatcatc aaaacgtcaa aagtccccat tccacctggg accatcgtca
     5521 gcattcctat caatgtagca atcatgaaca taggatagat cgaaagcatc gaaatatcta
     5581 ttctcatcaa cttgccgatg atcaaaaata ccgcaagggc tcccgtccat tgtccaaagg
     5641 atgccagtag cagaccaagc aagccttttg gataaaactc gaagaacaat gtctttctct
     5701 ttagataagc aaaaaagaaa atgactggtg ctaaaaaact accgccgaaa agccagatcc
     5761 aatagcttcg aaaaatactt tctgactgaa taaaaaagac atcaataaat gtaagaaacg
     5821 accatacaga caaaccagtc agcataaata gtgcgacttt tgatacagtg gctaaaacca
     5881 tttttctatt tgtctgcttt ccataaaaac tagcccgcag acttgctcct accacaccac
     5941 caaaaccggc gagattgttg atcgtgtttg ttacccacgc agcaagaaga aactcttttc
     6001 tttccatttt tggttttcct tgtttttcta agactctgat cgtcacccaa tcatacaaca
     6061 acatcggagt gacacccagc agtccagcca gaatcatgag caggatcgtc atgcggcttt
     6121 gttgtcccat cctttgccaa atatcttgcc aactcatgcc attagcaata tttgcgactt
     6181 gatttgccac aaagactaac acggagccga aaaaaatcag ttttaaaatc agactatgtt
     6241 cctttatcca ttgcagcatc aagtatcgtt tatttttcac attttcccat tcctaacttg
     6301 tatacttttg tgtcataccc caatgttatc ttatttattt taatttttcc actttagacc
     6361 tgttctttgc ttgcgatgat agaaaaagaa agatatccta atgctaaagg agaggataac
     6421 atgaaagtag gatttatcgg cacagggaat atggcacaag ctattattga tggtttatta
     6481 aaaaaaggaa cagtctcgaa agaagaggtg ttcgtttcca gtggacattt tgaaaatgca
     6541 caaaaatttg ctgctgaaac tggcgtccat gcatgcaaaa gtaatcagga agttgcatca
     6601 caatcagata tcgttctttt agccgtgaaa ccaaaaatca ttgaaaaagt attggaagaa
     6661 ctaagagaat atagccagaa agtcttattc atttctattg cttcaggaaa atcattaaca
     6721 gatttagaaa cagcgcttga agatcctcag gcctccatcg tacgtgtgat gccgaatgtc
     6781 aatgtcagcg taggagcagg cgtttctgcg atttgcaaaa atgaagctgc cacggaggaa
     6841 cagcttcagc aagctacgat gatttttgaa gcaatcggca gcgtctatcc tttagcagaa
     6901 tcagatttca gtacattcat tggccttgct ggcagctcac ccgcattcgt ttacatgctg
     6961 atcgatgcga tgggacggac tggtgtactg aatggtttgt ccaaacagac agccactgag
     7021 attgctgcaa aagcaattat gggttcttgt caaaaattcc tttcaagtga taaaaatcca
     7081 tgggaacttg tagaccaagt ttcttctcct ggcggtacaa ctgttgcagg tgtagttgcc
     7141 ttagaagaag caggattcat cccagcaatc atcaaaggaa tcaatacgac gatcgagaaa
     7201 gatcttgaga tgttgaataa aaaataatga tagcaatgtt ttttcgtgcc ttacaaaaaa
     7261 agaagagctg tggcttttgc cgcggctctt cttttgtatt ttttattatt tagcataatc
     7321 agttgcacga gtttcccgaa taacgattac tttgatgtgt ccaggataat ccagttcttc
     7381 ttcgattcgt ttacgaatat cacgtactaa acgaacagcg tctaaatccg agatttcttc
     7441 tggcttgacc attacccgaa cttcgcgtcc agcttgaact gcgtaacttg attctacacc
     7501 ggcgaagctg ttcgaaatat tttccagact tcttaaacga cggatgtagt tttctaatga
     7561 ttcacttcgc gcaccaggtc ttgctgctga gagcgcatct gctgcagcta caaggacaga
     7621 gataacagaa gtagcttcca catctccatg atgagaagcg atcgcgttga ttactactgg
     7681 gttttctttg tacttggcag ctaattccgc accgatctca acgtgagatc cttcaatctc
     7741 atggtcaagt gctttcccaa tatcatggag caacccggca cgttttgcta attggatatc
     7801 ttcgccaagc tctgctgcta agataccagc taattttgca acctcgaccg aatgcttcaa
     7861 gacgttttgt ccataactag ttctgaatcg cagacgtccg atgatcttga ttagatctgg
     7921 atgaagtgta tgagcaccga cttcaaacgc tgcctcttcc ccgtattcgc gaatacgttc
     7981 atccatttct ttacgagatt tctcgaccat ttcttcgata cgcgctggat ggattcgtcc
     8041 atcttgtatc agtttttcca aggtcatccg tgcgatttct cttcggattg gatcgaatcc
     8101 ggaaagaaca actgcttccg gtgtatcatc gatgattaaa tcgataccag tcagcgtctc
     8161 aagcgttcga atattacgac cttctcgtcc aatgatacga cctttcattt catcattcgg
     8221 aagggtaacg actgaaacag tcgtttctga aacttgatct gctgcgcagc gttgaatcgc
     8281 taatgataat aagtttttcg ctttgcgatc tgcttcttct ttggcacgct gttcagattc
     8341 ttttaccatt agagttaact catgattcag ctcttcttca gtcgatttca tgatgacatc
     8401 tcgtgcgtcc tcttttgata aaccagcaat tttttctagt tctttttgtt gatcttcaat
     8461 cattttttca acgtcttttt ctcgctcttc aattaattgc tgttttgaac taagtgcttc
     8521 ttctttactt tcaagtgagt tctcgcgttt ttctaacgag tcatctttac gattcagcgt
     8581 ttgttcgcgt tgaatcaaac gattctcttg agattttaat tctaacttac tttctctcag
     8641 ctcactttca acttcagaac ggtatttctg attttcttcc ttagcttcta acaatgcttc
     8701 tttttttaga gtttctgctt ctttttttgc actatcgatg attccagcag ctgatgattg
     8761 tgcaccagct atttctttct cgtgacgtga ctttgcgacc ataaacccta aaccaagacc
     8821 gacaattaaa ccgatgatag cgaggagaat gttgaatacc ataaaatcca cctccatact
     8881 atctttttca tttttatcat ctatacagct tctatgataa attattctaa actcgtcaat
     8941 atgcatcttc gcatatatgt gtacaaaaaa catgcacaac tttattctaa tgtttgtacc
     9001 ccgtagtgtc aactttaaat aaacaaagaa acggctttct acttattcat taaacgatgg
     9061 tttttaaaat aaatgacact agtttcatat aggtaaacct ttaaattgag aaacttttct
     9121 attaaattat tctttgtaga tgaacagata tttttatttt atgtacccac tcgtataatt
     9181 aaataactca cgatttcttt attcaataaa aaaacggcag gtcaaggaga gtacctactc
     9241 ttcagaccag ccgtttttgt ttatcataat ttttattctt ctactaaagg caattcttct
     9301 tgtccttttt catcttctgg gacagcgact tcttcaccaa taccataagc tgctctaact
     9361 agagcagaaa cttctgccat catttcagga tgattagcca tatagatttt agcgttttcc
     9421 cgtccttgac caatccgatc ttctttataa gaataccaag caccgctttt atcaacaatg
     9481 tctttttcta ctgccatgtc tagaagttct ccttcttgtg agatccctaa accatacatg
     9541 acatctactt cagcgatttt gaaaggaggt gctactttgt tttttacgac tttgatcttt
     9601 gtacggttac caacaatatc tgtaccttgt ttcaattgtt ctgcacgacg tacttccaaa
     9661 cggatcgttg cgtagaattt caatgcgcgt cctccaggag tgatttctgg attcccaaac
     9721 atcacgccta ctttttcacg gatttggttg atgaaaatag cgattgtttt tgttttgttg
     9781 atcgaaccag agagtttacg caatgcttga gacatcaaac gtgcttgtaa cccgacatgt
     9841 gagtcaccca tttcgccgtc gatttctgct cgtggaacta aagcagcaac tgaatcgact
     9901 actacgatat ctacagcccc acttgatact aaagcatcag cgatctctag accttgttct
     9961 cctgtgtcag gctgtgaaag aagtagttca tcgatattca cacctaattt ttgtgcatat
    10021 tgcggatcta acgcatgctc agcatcaatg aaagcggccg ttccgccatt tttttgtact
    10081 tctgcaatag cgtgtagtgc aactgttgtt ttacctgaac tttcaggtcc atatacttcg
    10141 atgatacgtc cacgggggta accgccgaca cctaaagcga catctaacgc aagagagcca
    10201 cttggaattg tagagatttg ttggtcgatt ttttcgccca atttcataat agagccttta
    10261 ccatagctct tttcaatttt tttcagtgca gcatctaagg ctgctttacg atcatctgcc
    10321 aatacgataa tcctccttat atttacaaat cagagcaaca tttcattatc cttatcatat
    10381 cttttttagt gaaaaaaagc aagaaaaaac cgaacaaata ttcgcattta tcattttctt
    10441 aaaagctgtt ttctcaaaag atcaaatcct tgcataaccg cattatatct gatagcagca
    10501 cgatctctgg caaagtgata tttttccgaa accacgccgt ctttagatga aatagcgatc
    10561 catactgttc ctgcagattg accttctaac gtgtccggtc cagccactcc ggtgaaagct
    10621 acactgtaat ctgttcttgc tatctcacgt gcttgaatgg ccatctgctc cacacattct
    10681 ttgctgactg ttccatattc tgctaatagc tccggatcaa tctccagaaa accagctttt
    10741 gtttggagcg agtaggtaac gaatccgcct ggaaatactt cagatacacc agcaatgtcg
    10801 cctaatgcgg cttggaaggc gccggctgtc aaactttccg ctgctgttac agtctgcttg
    10861 ttttctttca gtagttccac tacgactttt gctagcgaat tatcatcgcc gtatccataa
    10921 aaatattccc ctactcgttc ttggattttt tcttctaatg ctaaaagagc ttgattccct
    10981 gcctgaacat catttgtttt cactgtcagt cgtaatgtca cttcatttgg tttggcatat
    11041 ggtgcgatcg ttggattgat ctgtgtctca atcagatctt ttagctcagt gaccagtcgc
    11101 gattcaccaa tcccataaaa gcgcaaaact ttagaaatta gtttttcttc agaaggaaaa
    11161 cgttcctcta gtaaaggacg tacttgttcc acaaacattg gtttcaactc attaggcgga
    11221 ccgggaagaa ggatatacgc atgtgtatct gtttgataaa aagtccctaa agcaagccct
    11281 gtacgatttg gtagaggaac cccgccttcg ataatctggg actgttgcag attgtttttg
    11341 gtcatttttc gatgggttgt ttcaaaatat gccaataatt ttttgtatcc ttccgtattt
    11401 tgtatgagag acttaccgag gtgagccgca gttgcttctt ttgtcaggtc atcttctgtc
    11461 ggtcctaatc cgccacataa gagtatgagt tcacttcttg tttcagctag agcaatcaac
    11521 tcttctaaac gctgctgatt atctcctaca actgtttgat aataaacttc gatacctaga
    11581 tcagctagct gttctgacaa gaaagtggca ttcgtattca cgacttgtcc tagtaaaagt
    11641 tctgttccga cagctattat ttctgctttc attacttccc gctcccttat atagtatacg
    11701 ctgtattttt atattttttt atttaaaaag agggcaagga gctgtgacaa aaatcttgtc
    11761 acagctcctt gttccaaata aacagtgttc tcctgccaaa acctcggtca taagtcacaa
    11821 tatacataaa acatgaaaag ctgttttttg aaattatccc ttgtgactca gagttagacg
    11881 gcaggctcac aacctctcca ctaaacgata cttacattga tcctttgaac acaccgctgt
    11941 ttttgatgaa gtaatccaca cctgaataga tagtaaagat cagacaagca taaagcatga
    12001 tttgatctac tggaataccg atcaggctga atgggatatt attgataaac agcaagataa
    12061 tagcaaccat ttgtgtagcc gtcttgactt ttcccggcca agctgcagcc atgacttctc
    12121 catgttcgac aagtagtaaa cgcaagcctg tgacggctaa ttcacgacag acaatgatcg
    12181 ctactaccca accaggggcc tttccttggt caaccaacat gataaaagca gtcataacta
    12241 gcattttatc tgccaacggg tccgcaaact tgccaaaatt agtgacaagg cctcgcgcac
    12301 gggcaatttt tccatctaac cagtctgtga tactagctaa agcaaaaata actgcaccta
    12361 ctagctgtgt gacagccagt gtggtgtctc ctacgttaaa ttgtccccaa tctaaaggta
    12421 aacctacaac caaaatgaaa atcgggatca tacaaattct aagtacagtt aatttatttg
    12481 gtaaattcac actatcgact cctttcttcc ctattctacg tgactatcag cgaagtttcc
    12541 accgatatta tttacttttc caaatgaatt tattcaaagg agtattttta ttgaaattga
    12601 tctttttgaa ttaacgaaga aggaccgcga cacagatttg tcacgatcct tcttttttat
    12661 acctacaagt aaacagatgc gtaaactaat ttatacgatc attctgcgta ttgaagagta
    12721 aggttcacat tttttggact ctgattgttt tcccctggat taactggtac ggattctcca
    12781 ttcgctttga tcgttgcatt gcttgctgca ccaatcgtga tggtcgcatt tgttgctgtt
    12841 tccggtaaca cggtactctg tgtttcattt gctgccagtg tcccttgata aacataggca
    12901 ttgtctacta gcacacctaa ccatacacga ttgacagcag taaattcaag ggttacaggc
    12961 ttggtagcgt ctgtgatatt tactgcgatg gcactgccag aatcttctcc tgtagctatt
    13021 gccattttct tttcttcact tgattttgtt gttgattcta ctgtagaaga ctctttggtc
    13081 tgcgtgcttt gctctgagga agaggactcc tcgactgatc cttctacttt aagcgaagaa
    13141 gctgtgtctc cgattattgg atcagcttga cgatcttgcc aagtcatata gcccacgaca
    13201 acgacaatcg ccaaagcaac caatcctaat aaaatgacag gaagacttgt ccaaaagcga
    13261 cttggatgcg attcttcctg atgcattgct tttctagaag tacttactgt ttcaggctgc
    13321 ggacgttcag ggaccgatcc tgtcaatttg cttttcccat caaaaacatc gactaaatgg
    13381 tctccatctt ctcctacagc ttcagcgtat tgccgaataa atgcccgcac gtaaaattta
    13441 ccaggcaatt ggtcaaaatc atcattgtcg attgcttcca aataacgttt ttgaattttt
    13501 gtaatctgct gtaattcatc taaagatatt tttttattca ggcgtgcttg ccgtaatttt
    13561 tctccgatgg tttcaaaagc cactcgttca tcttctccag tctgtttgat tctccgaagc
    13621 atctgctgtt gcaacgaaag acgttatgtt tgggtgctgt gacaaaagtc atgtcacagc
    13681 tccttttttc cgaataaaca ttgttctcct gccaactcct cggtcacaag tcacaatatc
    13741 caataaatat aagaagctgt tttttgaact tgttccttgt gactcggagt taaacggcag
    13801 gctcacgacc tctttcttaa acggtgttca atcagctgtt cctcggtatt agctcaaaaa
    13861 ccggcaaaat ataaggagat atttttccgt
//
LOCUS       DASJST010000071        13671 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJST010000071 DASJST010000000
VERSION     DASJST010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.071"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..137)
                     /locus_tag="U4B05_001706"
     CDS             complement(<1..137)
                     /locus_tag="U4B05_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050847.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIETSLLVN
                     L"
     gene            complement(664..736)
                     /locus_tag="U4B05_001707"
     tRNA            complement(664..736)
                     /locus_tag="U4B05_001707"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(701..703),aa:Lys,seq:ctt)
     gene            1117..1686
                     /locus_tag="U4B05_001708"
     CDS             1117..1686
                     /locus_tag="U4B05_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN2050848.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1673..2533
                     /locus_tag="U4B05_001709"
     CDS             1673..2533
                     /locus_tag="U4B05_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2050849.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2723..3427
                     /locus_tag="U4B05_001710"
     CDS             2723..3427
                     /locus_tag="U4B05_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2050850.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3432..5267
                     /gene="walK"
                     /locus_tag="U4B05_001711"
     CDS             3432..5267
                     /gene="walK"
                     /locus_tag="U4B05_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN2050851.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5264..6580
                     /locus_tag="U4B05_001712"
     CDS             5264..6580
                     /locus_tag="U4B05_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050852.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6581..7450
                     /locus_tag="U4B05_001713"
     CDS             6581..7450
                     /locus_tag="U4B05_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN2050853.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7505..8314
                     /locus_tag="U4B05_001714"
     CDS             7505..8314
                     /locus_tag="U4B05_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2050854.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8417..9046)
                     /locus_tag="U4B05_001715"
     CDS             complement(8417..9046)
                     /locus_tag="U4B05_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN2050855.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9216..10508
                     /locus_tag="U4B05_001716"
     CDS             9216..10508
                     /locus_tag="U4B05_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2050856.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10584..11246)
                     /locus_tag="U4B05_001717"
     CDS             complement(10584..11246)
                     /locus_tag="U4B05_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2050857.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11471..11755
                     /gene="groES"
                     /locus_tag="U4B05_001718"
     CDS             11471..11755
                     /gene="groES"
                     /locus_tag="U4B05_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN2050858.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11806..13431
                     /gene="groL"
                     /locus_tag="U4B05_001719"
     CDS             11806..13431
                     /gene="groL"
                     /locus_tag="U4B05_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN2050859.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4570 a         2348 c         2924 g         3829 t
ORIGIN      
        1 tggagattga ctagcaggct agtttcaatt gctaaaaaga ctacttcagt atcccagact
       61 ttatataaat ctttgacaaa tttttctgat ggatcgtatt taaaccaaag cagatgttcc
      121 tctagctcga ttgtcatgtt tttcacctca acgaaagtat acgaactaat gtttgttttg
      181 taaaggataa aaatagggta gcaaccggta cccaaactag tacccattta tcaaaaacga
      241 cgaaatttat gtaaatagaa aaataagaat aaacattgat gtatcaacat ttatatatct
      301 ttagaacaaa tgaaaagggt ggttctcata agaaaaacat gttcgcactt ctaggcaaac
      361 caggatttga agaccttgct aaagaattga acgaacgcct gtaaaataag gatttgtcgc
      421 tagatttaaa aaatcagtga tagaaacatg tttgctttaa aatgaaacta gtgatagttt
      481 tagtgatagt gaacttaaaa aaagcactca atttgagtgc ttttttattg ttattaaaaa
      541 ttaaaaaact aaaatacata cccgaatttc atacccgaaa tataaaaaat gtcatcacgt
      601 acatcttttt cgaaactacc gaagtagcca aagatgtact gatgacgtgg gtttggcacc
      661 caatgggccg tgaggggctc gaacccgcga cccgctgatt aagagtcagc tgctctacca
      721 actgagctaa cggcccgtac aaaaagtact taataatagt agcactaaga attttaaaat
      781 gcaagactac atttaaagaa aatcagaaaa atctttactt ccttatagga aatcattcat
      841 agattattca catttgctcg ctattatata agcataccaa caaacaaagt tcttgcaaga
      901 gaacaaaccc taacactttt ttcatttttt actcctttcc cgccgtctcc ccggcgggtc
      961 tcctgttctt aacgataaaa gacaagaagg cttagccttc ttgttttttg ttttcatgga
     1021 atcccgctaa tcaaatcacc tagttaaagc aactagattg aatgtttatc ataagtctga
     1081 tatcatgttt atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg tcagcaatta
     1141 atggagtggg ggaaggaact agaaacattc cgtctgcctc actgggaaga actccctgac
     1201 ttagaacttt atatggacca agtcagaacg ttagttgatc gttatctttc acctgtgata
     1261 caaggagaaa agcatccatt gcttacctct tctatggtaa acaattacgt taaactaggt
     1321 ttgataccag caccagttaa aaaaagatat aacaaagaac acgtagcatt tttacttgca
     1381 atcactacgt tgaaacaagt attgactatt cctgaaatca aagaagggat attgttccaa
     1441 ggaaaaacag taggaattcg tgaagcctat aatctatttt gcgatgaaca agaagcagct
     1501 gtgtggatgg tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt cgaccaggca
     1561 acccctgtag aatatattgc tgtaagagcg gcaactttat cgtttgcaat gaaacttctt
     1621 gcggaaaaaa cgattgttct agaaaccgaa tatttaaaag aggagaaaac aaatgaaaaa
     1681 caataaaatc gcaattttag tagattcagg tacggatgtg ccagaagaat tgataaaaaa
     1741 atacgagatg tatatgatcc cattgaaaat tatttataaa gatcaggtat atacagataa
     1801 aattgacatt acctctgaag agatctatca acgtttgcca gaggaaatcc ctggtacatc
     1861 tttgccggat ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag acggttatga
     1921 aaaggtactt gcggtaacaa tttccagtgg attgagcggg acatataatg tggttcgttt
     1981 aattgcacaa cagcaagaag aattggaagc atatgtacta gatacgaaaa atattggtat
     2041 tggtgcaggt ttttctgcga tccaagcagc aaaatggctg gaagaaggag tagaatggga
     2101 tcaactgatc agtaatctga atgagttagt cgaaagaaca aaagttttct ttaatgttgc
     2161 gacattggaa tatcttcaaa aaggtggacg gatcggttta gttgcttcta ttgtaggaac
     2221 cgctttaaag cttaacccaa tcatctcatg taatgaagaa gggatttatt acacagtagg
     2281 aaaagctagg ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca aagaaaaaat
     2341 cggtaatgca aaggtcttca acttagctgt tgcccatggg gatgcgaaag aagaagcaat
     2401 tgaaatggaa gcgagactta aaagagagtt cccacaagca aaccaaatct actttggcca
     2461 gatttctccg gcattagtag tccacactgg tccgggatta ttgggtgtcg gtgtacaagt
     2521 tttagaagga taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt ttcctttttt
     2581 attgaagtta gaacaaaaaa taataaagta ttttgcactt tcattttaat tttctctttt
     2641 gttttatagg caaagatgaa acaaaagcag aaatacggta taatgagaag gtatgacaat
     2701 aatatataag aggtggctct ttatgaaaaa agttttagtt gtcgatgatg agaagccgat
     2761 ttcggatatc gtcaaattca atttagccaa agaaggctat gatgtttata ctgcatatga
     2821 tggggaagaa gccttggaaa aagttgctga ggtagaacct gatttgattt tacttgattt
     2881 gatgctacct aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa cttatgatat
     2941 gccgatcatt atggtaacag ccaaagattc tgaaattgat aaagtcctag gcttagagtt
     3001 aggcgccgat gattacgtga ccaagccatt ttccaaccga gaattagttg cacgtgtcaa
     3061 agcaaatctt cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag cacctgcgga
     3121 attgacgatt ggtgatttga cgatccatcc agaagcttac atggtgacga aacgaggaga
     3181 aacaatcgag ttaacacatc gtgaattcga actactcttt tacttggcga agcaccttgg
     3241 acaagtgatg acaagagaac atttattaca aacggtttgg ggttatgatt attttggaga
     3301 tgtacgaaca gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag ataatccaag
     3361 ccatccaaat tatttagtga cacgtcgtgg tgtcggttac tatttgcgta acccagaaca
     3421 ggagtaattg gatgaaaaga aaagtccgct tttttcgatc agtcaatttt aagatcgcta
     3481 ttacctttat tctgatttta ttgatctcga tcgaaattat tggtgcctac tttatacgtg
     3541 gtttagaacg ttccacgatc aatacattta ttaaagatat gaatcaaacg gtggaatcat
     3601 tagcaacaac aatcagcccg gaattgaatc gaaaagataa tgcagatgat gaagaggtaa
     3661 atgcgaatat caaaagattt attgaaaaca gtgcgactag cgatatcatc gaaatacggg
     3721 tcgttgacga aaaagggatc atccgaggaa caaccgatgt caatgaacaa agcgctgtgg
     3781 gaaagaaaaa tgattatgtt gatatcaatg atttcacgac taaacgttat gttgctttag
     3841 ataacgacga taaacgggtg aatatcaatg ttcaaccgat cctgtccccg accggcgatg
     3901 ccgttatcgg ggctctttac gtcaaaagta atatcgaaca aaaatattcg gaaatcaata
     3961 acacagcagt cattttcttt actgcctcac taattgcggc atttatttca atgatcgtat
     4021 ctgttttagt tgctcgttcg attacacagc cgatcggtga aatgagagaa caagccattc
     4081 gaatcgctag aggagactac agtcggaaag tcaaagtgca tggacaagat gaacttggac
     4141 agctggctga taccttcaat cagttagctg aacgtattga agaagctcaa gatacaatgg
     4201 aagctgaacg caatcgtttg gatagcgtgc tatcccatat gacagacggc gtcattgcga
     4261 cagatcgcag gggtaaagtc attacgatca atgatatggc tgtttccttg ctagatatca
     4321 aaaacgagga agcaatcgga caatcgattt tgacactttt agatatcgaa gaagaatata
     4381 cgttgcggaa attattagaa gatccaaacg aaatggtact tgaccgttca accagtacat
     4441 tggaaagtga tcagatgatc ttgcgagtag acttttctat gatccgtcgc gaatcaggtt
     4501 ttatcagcgg attagtagct gtcttgcatg atgtgacaga acaagaaaaa acagaacggg
     4561 aacgtcgtga attcgtctct aatgtttccc atgaacttcg tacaccgttg acaagtatgc
     4621 gtagttatat cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta gcaccaaatt
     4681 ttttgaaggt aacgctagat gaaacagacc gaatgattcg aatgatcaat gacctcttaa
     4741 atctatctcg catggataca ggtaataccc agcttcaatt agaatatgtc aatttcaacg
     4801 agttagtgaa ttttgttctt gaccggtttg atatgatggt gggaaatcag gaaaaaaatt
     4861 ataagatccg tcgtgaattt acgcaaagag atctttgggt cgaagtagat acagataaaa
     4921 tcatccaagt agtcgataat atcatgaata acgcgatcaa gtattcacca gatgggggaa
     4981 cgattacttg tcgcttatta gaaacgcata ataacgtggt tttaagtatc accgatcaag
     5041 gattggggat tcctaaaaaa gatctgaacc gagtgttcga gcggttctat cgagtagata
     5101 aagcacgtgc aagagcacaa ggtgggacag gacttggatt agctatttct agagaagtga
     5161 ttaaggcgca tcgaggcgct atttgggcag agagcaagga aggaaaaggg tcgacttttt
     5221 atatctcgct gccatatgaa ccttatgagg aggaatggtg ggaatgagaa tctctgaaaa
     5281 agtcgttcgt ataggactca ttttgatggt tgcgttaagc atatacttct cttatgcaat
     5341 ctggctgagc ccagcaggaa aaagttcaat caacgtagat gagacaaaca ctcaaatgat
     5401 cgagtctcaa agcaatcgaa aaacttccga agtctttctt ccgcttcatg ttacatggca
     5461 ccattcggga atgatccaag aaaccaacag tgaaaatctc gtgtaccgat tgcaattgat
     5521 catcgataaa gcaagatttg gaaagctttc tgaagtagtt tcaggagaca aatcacagtt
     5581 tgaacaggtg aaaaatctga actctggttt tgagttggcc tataatgcgc cttttttgct
     5641 aagagaatac aaggaagcat ttgatttaga tttagatgtg tcgaacttgg caaaagaaag
     5701 cgaaaatatg tattttacta gaatacaatt cgatcaagga aataaaaaaa tccgattttt
     5761 gaattattcg aaccatctcg tatatgaagc aaatatttcg ttgaattgga cagatatcaa
     5821 taaagaatta acagcaaaag acacaaaatg gacagaaatg acaacagaac catcgacgat
     5881 cgacacgcaa tatgctacga aacattcgat caaactgaaa aagtatagtt atatcctgtc
     5941 acagcaaccc tatacacttt ttagaaatgc ttttttccag aaaccagata gcgtgaaaaa
     6001 taatgatgaa agcaatgaat tgatttttta cggtgggaat gaaacaatgt ctatccattc
     6061 ggaaacgcaa gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa atgctagtgt
     6121 attttctgaa agtttcccct atattagcaa attaggcagt tctttaggga acctcagata
     6181 ttttgaccgc agtgacaata cgatcgatta tcggatattc gtggaaggct tcccggtttt
     6241 tgggacagac agcagaggac agattggtgt ggaagttggc ggtgaaacga atggttctgt
     6301 gcaagtgaat atccaaacca gtttgaatac gattcaagtg ccgatacctt cagacgaaga
     6361 agtagaactt gcaagtacga atgaaatcat ccagcagtta aaagctaagg gagcagatag
     6421 caacaagatt ggttcagtga tcgttggcta tacatggcaa aatgtcaaag aaacgaatcg
     6481 tgtcgttgat ctcttacctg aatggtatgt gaaatataaa gataactggt attcagctaa
     6541 cgaccttctg gaaaaattac ctggattgga ggcgaattaa ttggatttca aaaaaatcga
     6601 atggatattt ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca gttatcatga
     6661 cgcggtatac caagaaaaca atgtcattcg ctctaatcag actgaatcaa tccaacaacg
     6721 tctggctagc gacgatatca cttacacggg gaagatttcg tctgagaaca agcaaggcta
     6781 ttatcttagc gcggagcaga ccaatcttag cacttcatta gcgaattatc gcaaggctgg
     6841 taaaagcggc ttgctggcaa gtggtgtgtt tttagacggt gatgttttga cgaaatcttt
     6901 atccagttca gatgctgaaa agaacgtgat cgatccagat aaaattagta gttccttgga
     6961 agatttttta tctaacaaag acaatatttt attcggtaat gattatgtgt acacgaaaaa
     7021 cttttctcat ttagaagaag aaccactaga aatccaagca agccaagagt atgaaggcat
     7081 cccattcaat gatgatacag caaaaatcgt tctttcttta gaaaaaaacg ataatgatta
     7141 tagcatcacg aaatacatgc agacgcattt atctgatatc gagcagctgc gcgaaaagac
     7201 ggaactgtat acggaagagg aagcaatcaa tacattgtac atcaataaca aaataccaag
     7261 aggatcaaaa attttatggc gtcagctagc ttattcctgt attctgaaag tccgggaaaa
     7321 aaatgtgtat gttcctgttt ggcatgtagc gatcgaaaca agcgataaag tggtccaaat
     7381 tgagagtgtc aacgctttca gcaatacgat cgtgacaaac aatactgtac caaaagtgga
     7441 agatcaataa aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg gaagtaggag
     7501 tcaaatggct gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg gaagttcggg
     7561 taattcactt tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag gacttagcgg
     7621 taaaaaaatc acttccttga tggctcaaat tgatcggaca ccggaccaat tagatggaat
     7681 acttgtgacg catgaacatc gagatcatat acatggcgtg ggtgtcttgg ctcgtaagta
     7741 tcatttggat gtttttgcca atcagcagac ttgggaagcg atggaaccac tgattggaaa
     7801 tgtaccagtg gaacaaaaac atctatttga gatggggaaa gtcagaacat tcggcgatat
     7861 cgatattgag agtttcggcg tctcccatga cgcagcggct ccgcaatttt atcgtttcca
     7921 taaagaaaat cggtcattcg tcatgttgac ggacaccgga tattgcagtg attatatgcg
     7981 aggtgtcatt gaaaatgccg atggttattt gatggaaagt aatcatgatc ttgagatgtt
     8041 acgcatggga ccttatccgt ggaatttaaa acagcgtatt ttaggagata gaggacatct
     8101 ttccaacgaa gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga ccaaacgaat
     8161 ctatcttggc catcttagca aggaaaacaa tatgaaagaa cttgctcatt taacaatgga
     8221 aaatgttttg aaagaaaaag attttggcgt tggacatgat tttgaaatct atgatacaga
     8281 tccagatgaa gcaactgagc tattttcgat ttaaacagtt caatcaataa aaatataaaa
     8341 agcagaagat aaaatcaatc agggttggtt ttatcttcta tttttatttt tttagcatat
     8401 taaaaaaata accgtattat cgactgacta cactgatgtg ctgcttttca tgtttctcat
     8461 ccagtatctc atctactaat gcaatcgcgt aatctgccat gctgatcgta ctgtcgccag
     8521 cagcgtttct ttgtaaataa tcatcactta acaaataagt acctgtacgt ttgccatgtg
     8581 gatcaaataa agaagctgga ctgatataag tccaatgtag atttttagat tgtttcaatt
     8641 ttaacaatcc ttcgtacata ttgaaagctg ttggatagaa gggtgcattt ttaggtactt
     8701 ggtcaatcat acgagtcgtc ttttctgtat ctaagaataa cgaagaggca cccccaacga
     8761 caattaagcg tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa gaagtttgat
     8821 gcatttcttc ttttcctttg ggagcgttga aagcatcaac gacaacatca aaagatgcaa
     8881 gatcagtagt tgtcaactca aataaatcct tttgtataaa cggaacatct gatggcaatt
     8941 tttctggatg acgcacgatt gccgtcacgt ctaaatctct ggacaatgct tcgtctaaaa
     9001 taaatgaacc tgcatggcca gtagctccaa tgagtgcaat tttcataaaa aacctccttt
     9061 tttattcctg tccattgtaa gcagttgctg aatattcgtc aaagaatccg atttttgaaa
     9121 agctcagtgc gcattccttt catggttcgt gatcattggg tatagttaaa ttaagaaaac
     9181 tgattgataa aaatagaagt tgaaggagat aagaaatgaa agagtggtta gatcgtgcct
     9241 ttttaccaat gataacatct gttgtgaatc ttagaccctt aactgctttg cgtaatggaa
     9301 tgacttacac gatcccaata attattgttg gctcgctgtt tttcttattt gctcattttc
     9361 ctgtggatta catcgctcag tggatagaca aaacaggatt ttctatgtat tttacacaag
     9421 tataccaggc tacatttgat gtgatagcct tttggatcgt ttttgccatt gcatacgctt
     9481 atgcaaaaga agaagacatc gatcgcttac cagtcggcat aacagccttg tctgcttatc
     9541 tactgcttat tcagccacta aatactcaga tagatactgt ctggacaggt gctgaaggga
     9601 tactaggagc agttacaact gggctcatca tcggatgggg ctatgcacgt ttagtaagac
     9661 ttgcgaaagg gtggaagcaa agagagaaga tacctgaaaa tgtttggcag tcttttactt
     9721 cattgattcc aatcattgtt atagtcactt ttacgcttat cggttcagcg gtttttcaac
     9781 agttgttcag aatgactgtt gttcaaggag tatggacata tgtccagctt cccattcaat
     9841 cgttcataga cacgttgttt ggtgtgcttt tattaggttt ttttgttccg tttttatggt
     9901 tatttggtct tcatggatct gcgatggtca atgggttggt tagtcccgtc ttgcaagcga
     9961 attcattagc gaatgcagaa atcttagcct caggacaaga gttgacggtg gcaaatggcg
    10021 gacatatcgt tactcagcaa tttctcgatc agttcatgac ggttacgggt gctggattga
    10081 cgttaggggc tgtatttttt atgacggttt ttgcgaaatc gaagaaatac agagaattag
    10141 gaaatcttgc tcttttacca gcctttttca acatcaatga atcaattatt ttttccacac
    10201 cgatcgtgat gaacccgatg atggctgttc cttttatttt cgcaccggtc gtttcaggat
    10261 tgattaccta ttccgcttta tattttggat tcgttccttt atttacagca gtccaagtcc
    10321 cttggacaac accgccgatc ctctcgggtt tcttagtagg cggagtgcct gcagcgattt
    10381 tacaaggtat cgtgttgtca attagttttt tcatttattt cccatttttg aaaaaaatag
    10441 attctgcaaa ctgcaaaaag gaaagacaga caaaaaatga tggagcagca gaaaagatga
    10501 acgactaaaa aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc aatctaggat
    10561 gacaggagta tttatagtca tgtttatact agcccttttt ggacagctaa ttgaacaaga
    10621 acaactaatg tgttcattcc tacgtgtgtg atcatcgatg tccagatccg accagttgct
    10681 ttgtactcta ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg accatcatta
    10741 tgagcaaacg caaacagcaa agagctgatc actgctccga cccaaaaatt tgaatatcga
    10801 ctgatgatcc ccagtatact gcggcggaat acgaactctt ccatgatggg tccagcaaca
    10861 gttgtcgcca aaataaaagc gggtgcttcc atgatcatct ggatgatatt ttgtgtattc
    10921 tcggaagggg ttgctttgcc aaacaataca gattctaaca tgacagcgat gctttgcaga
    10981 atcaatgcga caaagattcc aaccaatcca taaataacaa tctttggagc agaagctttt
    11041 tttaccgctg attcgatcgc taagggttca cgttgcttaa aataaaaaat agctaggaca
    11101 acggcgccta gtatatacat gactgcagtt gctgtgatca attcagacgt agattttaca
    11161 agtcctatcg aagctaggat caagggtgag ataaaaatta gaccatagca aaaaatgctg
    11221 aggaagctat atttttttaa agacatgtaa ttcctccttt taagggataa caagcatgca
    11281 aggcaagctt atatcgattg atttcaatta tatcataatt tcaataagca gctgtcagaa
    11341 taaaatttct tttctttgat ttaaaagctt gcaaaaaaga gggagaatga gtattattaa
    11401 aaatgtaggt tagcactcga aggttaagag tgctaacaga ttaaaaaaag agttatcgga
    11461 gggatttatc gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg caaaagaaga
    11521 agaaaaaact gtcggaggaa tcgttttagc ttctgctgct aaagagaaac cccaaactgg
    11581 gacagttgtt gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag ttcctgccgc
    11641 tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag taaaatacga
    11701 aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg gcgattgttg agtaaatttg
    11761 gaagataaaa aaataatcaa aaataatcaa tgaggtgaat caatcatggc aaaagaattg
    11821 aaatttgcag aagacgcacg cgcagctatg ttacgcgggg tagataaatt agcagataca
    11881 gtgaaagtaa cattaggacc taaaggccga aatgtcgtac tagaaaaatc ttacggctct
    11941 ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga agatcatttt
    12001 gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga tattgcagga
    12061 gacggtacaa cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg tttaaaaaac
    12121 gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc aacaaaagca
    12181 gcagtcgaag aattgcataa tatctcaact gttgttgatt caaaagaagc cattgcacaa
    12241 gtagcagctg tttcttcggg ttctgataaa gttggacatt tgattgctga tgcaatggaa
    12301 aaagttggaa acgacggtgt catcacaatc gaagaatcaa aagggatcga aacagaacta
    12361 gatgttgtag aaggtatgca gtttgatcgt ggttacttat cacaatatat ggtaacagat
    12421 aacgacaaaa tggaagctgt tctagaaaac ccatatatct tgatcacaga caaaaaaatc
    12481 tctaacatcc aagatattct gccattgtta gaacaaatct tgcaacaatc acgtccattg
    12541 ttgattattg ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt gaacaaaatc
    12601 cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg tcgtaaagca
    12661 atgcttgaag atatcgctat tttgacaggt ggtacagtaa ttactgacga tctaggttta
    12721 gaattgaaag atgtaacaat cgaaaactta ggaaatgctt ctaaagttgt tgtagataaa
    12781 gacaatacaa cgatcgtaga aggttctgga gaaaaagaag caatcgaagc tcgtgtgcaa
    12841 ttgatcaaaa accaaattgc tgaaacaact tctgattttg accgcgaaaa attacaagaa
    12901 cgcttagcta aattagcagg tggtgtagct gttgttaaag ttggtgcagc tactgaaaca
    12961 gaattgaaag aattaaaatt acgcattgaa gatgcattga atgctacacg tgccgcagta
    13021 gaagaaggaa tggtctcagg tggtggtaca gctctagtga acgttatcag caaagtttct
    13081 gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg tgctttagaa
    13141 gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat tgttgataaa
    13201 ttgaagaacg tggaactagg cactggtttc aatgctgcta ctggagaatg ggtaaacatg
    13261 gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca aaatgccgct
    13321 tccgtttctg ccttgttatt aacgacagaa gcagtcgttg cagataaacc agaaccagca
    13381 gcgccagcag ctcctgcaat ggatccatca atgatgggcg gtatgatgta agaaaaagca
    13441 acttgcttat ctacaactga tcattagtga aagacagata aaaagaggct ggaacagaag
    13501 cgtttaactc caagaaataa gaagaaattc acggaaattg cttttcaaat ttttgtgaat
    13561 ttcagcttat ttccgaagga gttgcttctg cttccgctgt ttatacgtgt ttagagcgtg
    13621 agacaaaagt gttctttact tttgtcccac gctctttttt gtgtgtaatt a
//
LOCUS       DASJST010000072        13659 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJST010000072 DASJST010000000
VERSION     DASJST010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.072"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(275..967)
                     /locus_tag="U4B05_001720"
     CDS             complement(275..967)
                     /locus_tag="U4B05_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2050860.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1404..1973)
                     /locus_tag="U4B05_001721"
     CDS             complement(1404..1973)
                     /locus_tag="U4B05_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2050861.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2047..2370)
                     /locus_tag="U4B05_001722"
     CDS             complement(2047..2370)
                     /locus_tag="U4B05_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050862.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2524..3336)
                     /locus_tag="U4B05_001723"
     CDS             complement(2524..3336)
                     /locus_tag="U4B05_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050863.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3690..4538
                     /locus_tag="U4B05_001724"
     CDS             3690..4538
                     /locus_tag="U4B05_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN2050864.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4683..5624
                     /locus_tag="U4B05_001725"
     CDS             4683..5624
                     /locus_tag="U4B05_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN2050865.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5614..7785
                     /locus_tag="U4B05_001726"
                     /pseudo
     CDS             5614..7785
                     /locus_tag="U4B05_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7897..10068)
                     /gene="copB"
                     /locus_tag="U4B05_001727"
     CDS             complement(7897..10068)
                     /gene="copB"
                     /locus_tag="U4B05_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN2050866.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10088..12274)
                     /locus_tag="U4B05_001728"
     CDS             complement(10088..12274)
                     /locus_tag="U4B05_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN2050867.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12274..12483)
                     /gene="copZ"
                     /locus_tag="U4B05_001729"
     CDS             complement(12274..12483)
                     /gene="copZ"
                     /locus_tag="U4B05_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN2050868.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12496..12936)
                     /locus_tag="U4B05_001730"
     CDS             complement(12496..12936)
                     /locus_tag="U4B05_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN2050869.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13010..13549)
                     /locus_tag="U4B05_001731"
     CDS             complement(13010..13549)
                     /locus_tag="U4B05_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2050870.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3846 a         2847 c         2420 g         4546 t
ORIGIN      
        1 acatttgtat tttatcaaag ttttattata cttgtcaatt tgtgtacatc ctaaaatata
       61 agctatttgt ttacttctgt agaatatata ctttgttgtt agataagttg cacatgttta
      121 tagaatctaa ataataaatt tttatgtacc gcccctcaaa gtgaactgaa ccccaaaagt
      181 tgaactattt aatggactgt ttccgatatt ctactggaga tagtccatta aatagttcaa
      241 cttttggggt tcagttcaaa gaggggggga ttttttatcg ttgtggaata tttaaatacc
      301 aacgcttgtc atgaaaatct tgtgctccac ccttagtatt tccttctgga tcgtttgtcg
      361 cacgcatcat gacgtatact ttcttattag ggaaatcacg catattgaaa gatacatgat
      421 agccaacatt tcctaaagta ttataagctt gatttacgtc tggtctataa atcccatcag
      481 ctcttactct agctagttct tttccagtat tgtagtccat aatgaaaata tactcgtatt
      541 tataattagc aatgtgccat ccagctacat gcaagtttgc gttttcgatt tctccgaact
      601 gatcaatgtg ggcgtaattt gttccatctg tcagcgtaga atttgcagca cctgctcgag
      661 ttggatcaat tactggttta tcctccgaag tagttggatt atcatccgta aaaccatgag
      721 ccaaatcata ggctagtttt tctttactta cacccatttc agaaagataa ccgtaaggat
      781 ctgtatgatc gccccaaata ttttgtgtta cccataaatg cgatttgatt cctggttggt
      841 tataaggagt gtctaatgtt aatggaatac catatttcat tgctgaatct cttgccaatt
      901 caacgtatac tttatagttc ttttcaaaag tttctttatc atgtgtatgt tgtaactcga
      961 tttgcacagg acttttggct cacgaggttg tcctactcta catcttgctg atagatatgc
     1021 tgaaaggaaa acaccaacat tatgttttgc tttttcagct agaacgagac aagcaggaat
     1081 acctttagaa gatatcaaag gttttttagg tcataaagat gtttccacta cacaagtcta
     1141 tgcccatatt tctcccgaag tgaaaaaaag atcaatgaat caactagaaa actatataga
     1201 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
     1261 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
     1321 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
     1381 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
     1441 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
     1501 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
     1561 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
     1621 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
     1681 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
     1741 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
     1801 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
     1861 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
     1921 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
     1981 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
     2041 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     2101 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     2161 atcgatgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     2221 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     2281 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     2341 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaagtgc
     2401 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     2461 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     2521 aagtcattct tggtaaagta cttttgtcag ctttgatttg ctttcgctct caaattcgct
     2581 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     2641 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     2701 tgctagataa tttttttctt ttattggcaa aatctgctcc tctctaaata gcaattcagg
     2761 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     2821 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     2881 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     2941 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     3001 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     3061 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     3121 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgaa tgggacagtc
     3181 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     3241 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     3301 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     3361 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     3421 tatttaaata cgcaaatctt cctcattatt ttttatttta ctcaatttaa tcgtattctc
     3481 atttcaaaac gaatgtactt taaaaaaaca taataagatc actttgcttg taggatgtat
     3541 tcccttccat tacaatgaaa taaatagcga ttttatgaga aggtgatgtg aaaatggaag
     3601 aaaaaataga agaatccaaa caaaaaatcg atcagttgat ataagaatta gaaaaagcgg
     3661 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     3721 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     3781 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     3841 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     3901 ttaccacgat cttgttagca gatgagatca atcgtacgac tcctcgtact cagtcagcac
     3961 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     4021 atcatttttt tgtcttagct acgcaaaatc caatcgagta cgaaggaaca tatcctttgc
     4081 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     4141 acgaactttt gctacttttg gataaattcg aaaaagtgtt ggataactta aatgaagtat
     4201 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     4261 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagtcatgct gccatccagc
     4321 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     4381 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     4441 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc
     4501 atactcttgt tgcacaaatt cctattcctg tgaggtagat tgaggtagac aatggaaaaa
     4561 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     4621 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     4681 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     4741 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     4801 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     4861 aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt tggcagccaa
     4921 aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat agtctaggac
     4981 ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg cctgcttttt
     5041 gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt
     5101 accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat tctttcaacc
     5161 gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa tacgattatc
     5221 aagaagaaga atgttctcct cttttttgct tttggggttc gcccagtcca aaatttgaac
     5281 ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag ccggaacttc
     5341 tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat tttgcttctt
     5401 tggaagctgg cgatgagaaa gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa
     5461 tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa acattgtcaa
     5521 aagatcgaac ggtttatctt gtttatttcg tagaaaaaca actcatggtc caagaaaaag
     5581 acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaacggga aaaatggctg
     5641 cgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt agtagctaat
     5701 caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct tattgtctgc
     5761 tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat aggttgtttg
     5821 tttttatatt ttccttcgaa gacattttcc atattatgga tcttacagtt tttttcgtca
     5881 gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc agaaaagaca
     5941 gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt ggattacgac
     6001 tattggtacg caccctttct ttctttgatg gcttacttca tgatgcttac cgtttttcgg
     6061 aaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt gtatctggca
     6121 gcaaggcaat ttttctccat ccggttgttg tcgggaaaca ctcgattcca gtcattgact
     6181 acagttctgc ttatttgttt cttaactttt agcgctatct tgcctttata tcttccgaaa
     6241 gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg
     6301 ctttatgaca cactccatga gtatcaatta accggaagtg caagaacagg cttcagtgag
     6361 aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt tacagcgaac
     6421 caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg ttctggatgg
     6481 atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga catcacgagc
     6541 taccaaaccg ttatagaaga caaagaaaac attacattga ccctaaaaaa acaagttttc
     6601 ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag tggacaaacg
     6661 ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc gacacgttct
     6721 gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag cagtcagtta
     6781 aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa
     6841 cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat tcgtaaagtc
     6901 gaagctgtct aacagtattt aaaaacaagc ggaaatttcc gctattccaa aacggatacc
     6961 gggcatactc caaaagaaca cgattatgtg gattatttcc tctttgattc aaaagtcggc
     7021 tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg tatcccagct
     7081 cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca agggaatgag
     7141 gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt tcctggattt
     7201 ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc acaaccagtt
     7261 gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag cgaaaatatg
     7321 gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac aacactcaca
     7381 ccagcttata aaatcgtctt attcagcctt ggagtgtgtt tatccgtctt gttgttcttt
     7441 ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct tcattttttt
     7501 gcttctacta atacgattgc atatcaaaaa cttcttaacc ctttggaaac attggtaccg
     7561 cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga attcaagcta
     7621 gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg acgacaagca
     7681 gatccggtaa aattcgataa aaaaacctat ttagaaataa cgcatataat aagaaaaatg
     7741 gtatacaaaa aaattctgac aaaagggagc cgcttcaaaa aataagataa aatttctgga
     7801 gaatctactt ctggaatgcc gagaaccagc tgattgagta tcgattactc gaaaaaaagg
     7861 ttgtgacacg ttttttgtca caaccttttt tcattattat ctgattttca atgtcaaagc
     7921 atttagagct acaactaccg tacttaacga catcaagaca gcaccaactg ctggacttaa
     7981 tatgatacca attggcgcaa gaattccggc tgctagcgga attgcaagaa tattgtaacc
     8041 tgccccccac cagaggttct ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa
     8101 atgcaaaata tcttttggat cgctgtttgt caatacgaca tccgcagaat cgattgcgat
     8161 atctgtccct gcaccgattg ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt
     8221 gatcccatca cccaccatga ttactttttt cccttggtca aggtatttct ggacaacagc
     8281 ttctttatca tctggtaata aaccgccata atattcttcg atccctaaat aatctgctac
     8341 tgcttgggca gcgtttttat tgtcaccagt aagcatgaca ggagtgattt ttctttcttt
     8401 caatgttcgg ataaattctt ttgcttctgt ttttactacg tcacctaaag caacgattgc
     8461 agctagatga ttttctaaaa tcaggaagct gatggtattt ccttgttctt ggtaaggttt
     8521 caataatgct tgttctacgt ttaatccgag acgctcaact tctttttcat taactatttt
     8581 cacttgttgt tttttgactg ttgcctctag cccgactcct gaaagatttt ttagattttc
     8641 agcttgataa ggcttgattt catgttcttt caaataattc ataatgccaa cagccaatgg
     8701 atgattggca ttcgcttcca aggctcctag atattgaagg atttcatttt tattgaacgc
     8761 atcatcaagt acttcgacac ccgttacagt aaattttcct tctgttagtg ttcctgtttt
     8821 gtccagcatg atcacatcta gatcattggc ttgctccagt gcattgcggt ttttcaaaag
     8881 taatccgttt ttagctgcta ttgatgtgga acgagcaaca actaatggga tagctaaacc
     8941 tagagcatga ggacaggcga tgataaagac tgttaccata cgttctaatg cattaggaag
     9001 atctgtcaag aacaaccagg cgatgaatgc caggataccc acaattaatg cgacataaaa
     9061 cagccatttt gccactttgt cagataatga ctctagcttg gatttttctc cttgtgcttt
     9121 tcgcaccatt tccataacct tcgctagata accattttca ccagttccag ttacttcgat
     9181 ttcgatcgtt ccatccccat tgatagaacc gccaatcact ttgtcattgg cttgtttttt
     9241 gattcctctt gattcgccag tcacagctga ttcgtcaaca attgtttcac ctttcaagat
     9301 tacaccatct gttggcattt tatcaccaga ccggacgatc aaacgatcac cttcgtttac
     9361 ttctttcaat gaaactgtct cttcttttcc atctttcgtc aagcgtttca ctgattctgg
     9421 cagtaattct gctagttttt gtaaagcatt gctggcattc gacacagcat tcatttcaat
     9481 ccagtgacct agtagcataa taacgatcaa agtagccaat tcccagaaga aatccatgac
     9541 gtgtgtatgt ggattgagca gatttgcgat aaacgaatag acactataaa tatatgcaac
     9601 tgtaatcccc atcgcgatca gtgtcatcat agcaggactt ttttgtttca attccatctt
     9661 agctccactt aaaaatggct gaccgccata gataaatagt atagttgcta agatcaatac
     9721 gagccaatca gaaccaggaa acgttacttg aaatggaaat tccattccca tcattggtga
     9781 aaaaagaatg attgggatag ctaggataag cgatagccaa aatttttgtt tgaaattccc
     9841 catgtgcatg gaatgatcca tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat
     9901 ttgtttgctg tttgctatat ctccatggtt atgtcccatg tgcatggaat gatccatctg
     9961 actatggtca tgtttcatct ccatctgttt ttcatgagat tctgtacttt ctttgtttac
    10021 tacttctttt ttatgctcat gttttttctc catatcgttc ccattcattt ttactccatc
    10081 ccttctctta ctttttcatg tgtcgattta agcacaggga gttcaacaat acactgacgg
    10141 aactaaacgc catcgctcct cctgcaatga ttggattcaa aaagcctagt gcggcaaaag
    10201 gaataccaat cgtattatag acaaaagccc agaaaagatt ttgtttgatt tttctcaatg
    10261 tcaacctaga caactcgatt gtttgtgcaa tggatgccaa actgctattc atcagcgtca
    10321 catcggctgt ttccatcgca atgtccgttc cacttcccat tgccataccg acatcagcta
    10381 atgctaaggc cggtgcatca ttgattccat cgcctgccat accgactttt ttgccatctt
    10441 tctgaagttt ttctacgtag gctgctttat cctctggaag tacttcagca aagatatgct
    10501 ttggatcaat accaacttgt ttaccaatcg tttcggctgc cagtttgttg tctcctgtca
    10561 gcataaaaac atctaatcct ttattctgga gctgcttgat tgcttgtttg acttctagtt
    10621 tgatttgatc agccacggca atcaacccga tcacttgctt ttcatcagct aaaaacatga
    10681 ctgtttttcc ctgctgttct aattctaacg cgtgttcttg gtattcatca aatgataggt
    10741 tcaattcaat taatcgttta cgtgtacccg caaaatagcg tactccgtta atcgtaccgc
    10801 tgattccagc acctgggtgg gctgtaaaat caatcatatc gtatgttgct gcttcttgca
    10861 gttttccgta ttccacgatt gctttaccta gtggatgttc agaagcttgt tctaaagtat
    10921 aaaaaataga taggacttct ttagaaccga ctagatctgt aactttgggc tttccttctg
    10981 taatcgttcc agttttatcc aaaacaatgc tgtctaagtg agcagctgct tctaatgctt
    11041 ctccgccttt aattaaaatt ccatttcgtg caccaacacc cgttcctacc ataatagctg
    11101 ttggagtcgc taaacctaat gcacatggac acgcaatgac taatacagag acactatgga
    11161 tcaatgcgag ttgccaatca ccagtgatca aacctgtagc tatgagcgtg ataaacgcga
    11221 tgactaatac gatcggaaca aaaatccctg agatccgatc tgcgattttt tggattgggg
    11281 ctttgctccc ttgagcatct tccaccattt gaatgatttg cgctaatact gtctgcttgc
    11341 cgacttgtgt aacttccatg tgaagtagac cattcgtatt gattgtcccg ccaaagagcg
    11401 tgtcatccgg attcttttct acaggaagat tttcaccagt aagcatactt tcatctatcg
    11461 cactgcttcc tgaaataatt cgaccatctg ctggaatctg ctcaccagga cggatgacta
    11521 gttgatcacc aacgactact tcttcaatcg ctaacgtttc ttctttcccg tttcttatca
    11581 cttgggcggt ttttgtttga agagacatca attgtttgat tgcattacct gttttcgttt
    11641 ttgctgtatg ttccaaatat ttgccgagca aaatcagcgt gatgatcata ctgctgcttt
    11701 caaaataaag atcttggggg tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg
    11761 cagccgatgt tccaattgct accaatacat ccatatttgg tgctttcgtc ttgattgcat
    11821 ggtacgcacc tttataaaaa cgagcaccga cataaaattg gacaggagca gacagcacta
    11881 actggacgat tggaaaatga aaaaagtgaa caatcgccgc atggcttcct aacatcatag
    11941 caatcattga caacatcaac ggcaaagtca aaaccgtact caatataaga tcgaatagca
    12001 tttttttcaa atacgcttgc ttttcttctg caattttttg tttgtgggct tcatcgtaca
    12061 aaattgcacc atagccgatg ttttctacgc tttggattaa tttttcagtg gtcgtccctt
    12121 catatttgac acttgctttt tcagtggcca agttgactgt ggcattgacc acaccaggtt
    12181 gtccgttcag ctctttttcg atcctagcag aacaatttgc acaagtcata cctgtaatga
    12241 cgaacgtatc cattttggtt tctgctgttg ccatcaaatc acctctgctt tataacccaa
    12301 tccattgatt gcttgacaaa tcttttcgga ttgaactttt gtttcatcaa acttgaccgt
    12361 accgtttgct tttttcaaat ttactttcac tttttgcacg ccgtcaagtg cgcttactgt
    12421 ttcttctact cttgctgcac aatgattgca gctcatacct tcgattgaaa attgttgttt
    12481 cattttttct tcctcctatt gatgttgttt acattcacat tgacctggga tacagttaca
    12541 ttcgatcgta tctactggtt gcttttgttc cagttgtttc aaaatctcat tgatatcttc
    12601 ttgagtcaaa gtggcttctt ctactagatc cgcaatcgtc tctccaatcc tttttgcaca
    12661 gatatgggaa aaaagatttt ccgtcgcact gcgaactgtt tccatctccg agacagctgg
    12721 ataataaatg aatttttttc cttcttgttc tgtccagatc acttcttttt ttaccaaacg
    12781 acccagcaaa gttttgatcg ttgctggttt ccaatccatc gaatcagcta agatctgcgt
    12841 gatttcttgg gctgtcgtct gtccaagtgt ccaaataaca cgcatgatct cccactctga
    12901 atcactgatc tttactggcg ttttgacttt ttccatttat ttcacacctt tcgtttacaa
    12961 atgtaatcat tatcttatga atacagatta cacttgtaaa caaaaactgt caattatttt
    13021 tgctctctat tttcaattta gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga
    13081 tcagtttatt tcttgcatta gctacttgga tcagctgctg tattttcggt tcattgtttt
    13141 cttcaaaatg ccagataaaa gataaaaatt ctaagtattc cctgtttagt ttctccgaaa
    13201 atgattcagg catatctggc cgagattttt tgtttatctt tctttttaga gaacgccaaa
    13261 ctactcggta gatggcgaga taacgcggca ctttaagcca aatgatctta tctgcttctt
    13321 taatcctttc ctctaatgta gaagtataat ttccttcaac gatccatgac ggattagata
    13381 ccataaacaa ttgctgttct tgcaaaaacc acttttttgc ctgaatactg tagtctgttg
    13441 tatgccataa aaggtctagc ggcagtagcg gaaagtcggt gatttctttg atcttccttg
    13501 cgagggtcga ttttcccgac ccactttgtc caatcacgat gatcttcatt cttctccctc
    13561 acttctcttt ggaatttggt tcgatcatac cacttataac gccgcaaaaa aacaagggac
    13621 tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataa
//
LOCUS       DASJST010000073        13551 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJST010000073 DASJST010000000
VERSION     DASJST010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.073"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1020)
                     /locus_tag="U4B05_001732"
     CDS             complement(73..1020)
                     /locus_tag="U4B05_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN2050871.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1210..1479
                     /gene="rpsN"
                     /locus_tag="U4B05_001733"
     CDS             1210..1479
                     /gene="rpsN"
                     /locus_tag="U4B05_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2050872.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1655..2017)
                     /locus_tag="U4B05_001734"
     CDS             complement(1655..2017)
                     /locus_tag="U4B05_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2050873.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2028..3149)
                     /locus_tag="U4B05_001735"
     CDS             complement(2028..3149)
                     /locus_tag="U4B05_001735"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN2050874.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3165..3515)
                     /locus_tag="U4B05_001736"
     CDS             complement(3165..3515)
                     /locus_tag="U4B05_001736"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN2050875.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3645..5156)
                     /locus_tag="U4B05_001737"
     CDS             complement(3645..5156)
                     /locus_tag="U4B05_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2050876.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5478..6830)
                     /locus_tag="U4B05_001738"
     CDS             complement(5478..6830)
                     /locus_tag="U4B05_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN2050877.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6983..8059)
                     /locus_tag="U4B05_001739"
     CDS             complement(6983..8059)
                     /locus_tag="U4B05_001739"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN2050878.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8255..10000
                     /locus_tag="U4B05_001740"
     CDS             8255..10000
                     /locus_tag="U4B05_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN2050879.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10280..10717)
                     /locus_tag="U4B05_001741"
     CDS             complement(10280..10717)
                     /locus_tag="U4B05_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN2050880.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10701..12074)
                     /locus_tag="U4B05_001742"
     CDS             complement(10701..12074)
                     /locus_tag="U4B05_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050881.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12396..12884)
                     /locus_tag="U4B05_001743"
     CDS             complement(12396..12884)
                     /locus_tag="U4B05_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2050882.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12906..13508)
                     /locus_tag="U4B05_001744"
     CDS             complement(12906..13508)
                     /locus_tag="U4B05_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2050883.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3875 a         2941 c         2457 g         4278 t
ORIGIN      
        1 aagctaaaac attttttgtc tttgccccta caaaaatgtt tcagcttttt tcgtttttta
       61 ttcctgtatt tcttattttt cgaatgacag gatgattttc ccagcaggag gatgtccttc
      121 aactagctga tgtgcttgga tgacatgctt aagtgaagca gacagattca ttgaaataaa
      181 aacatgaaag gctccttttt ggtaggcttt gattatgcca gcaaaggctt ctgcatctag
      241 atattccttt cttggaacaa acgattcata aaagccttcc tttttttgcc gcaaatctag
      301 atctgggagg tcatttaatg caacatacct gccgcctggt ttcatgatct ggatgccggt
      361 ttctcctttg atacttcctt tcgtcgcatc gattacaagg tctgcctgat cagcaaattg
      421 cagacctgga tttgttttat cataagcagc aaatgctgaa gctcctaatt tttttacctt
      481 ttcctcattt ttactggatg cactggttaa gattcggatt cccttttcat ggagaagctg
      541 gatcagactt gacccgaccg cacctgatgc tcctagtatc attacagtat ctgtcggctg
      601 gatttccgtc aaatggttga ttagattata agccgtaatt cctggcgtga ccattccggc
      661 tgcttcttcc caactcattt tatctggaat ttttgctact tttgctgatg gcaacacgac
      721 ttcttctccg taagtcccgc cgactgcatg gacagctact cgatcaccta catgaacagt
      781 tgtgacatct gaaccaatct ctgtcacaat accagcgcca tcattacctg gtacataagg
      841 gaatttcaat gttcgtatct ctttcatttc tcctaaacgc aaggcaacat cataaggatt
      901 cacactgaat gcttttattt ctacacgaac atgtccagaa ccgacttctc tgggatgagc
      961 atcgatttct tcaaaaacat cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat
     1021 ggtcaccatc tccctttttc tttaagttta cgtttatgcc tttattttga caaaaaaaat
     1081 gtcttttcgt tttggcgcat gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt
     1141 taaattattt atctaaaagc gtaaagatta cgatttacgc taataaaaaa taattggagg
     1201 aatttgttga tggctaaaaa atcaaagatt gccaaagcaa aaaaacagca agaaacgatt
     1261 gaacgctatg ctgctttacg ccaggaatta aaagcgcaaa aagattacga aggcttgcgt
     1321 aaacttcctt tagatgctcg tccagaacga ttgaaaaatc gtgatttaat cgatggacgc
     1381 cctcgcggat acatgcgtaa attcgggatg tcccgtataa aattccgtga attagcacat
     1441 aaaggtttga ttccaggagt aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta
     1501 aaggaggagg gcgcatggac cactccttgg atcatcaaaa aagatacgtt cgcttcttct
     1561 agcagaaatg ctggtactag aagcagacgt atcttttttt acgcacagct ggtccttttt
     1621 tatatgtaaa ggctgcatgg ttacttcgct atatttattt agtaacagga gtcagtgctt
     1681 cgatacccac actaattttc aatgctcggt caacttctgc cataatgggt cgatccaaat
     1741 ggcagacctt ttctttcaat cttgatttat cgatcgttct gatctgctcc aacaggataa
     1801 cggagtctct ttctattcct gttccagtag ctttgatccc aatgtgagtc ggaagcttag
     1861 gtttagccat ttttgccgtt atggccgcaa caatgatcgt cgggctaaaa tggttaccta
     1921 gattattttg aataaccaaa actgggcgtg ttccgccttg ctccgatccc acgactggag
     1981 aaagatcagc aaaataaata tctcctcttt ttaccatagg atcctcctta ttcttgatat
     2041 tctcttggta tccgctgtcc taatccgcaa gccacttcat agtggatcgt acctaattga
     2101 tctgctatat cctgcatagt gatttctttg tctgcatttt tgccgataag cgtcactttt
     2161 gttccgacag gaaattcttg aggcaatcgg atcatcagtt gatccataca tacacgccca
     2221 atcgtttcac agtagttccc ttctaccaat agagaaaaac cctgcatctt tcgtggccag
     2281 ccatctgcat aaccgatcgg tatcgttccg atccactctg cttcaggagt gatatatgtt
     2341 tctccatagc cgattccttc acctgctggg agttttttga cttgaatcaa ttcagaaacc
     2401 agctcaagtg ctggctgcaa tggataaact tccggtagtg cgtgcccaga gggattaagt
     2461 ccgtacattg ctactccata acgaatcata ttgccgattg tttcatcatg ccataaagcc
     2521 gttgcactat tgctgacatg tacatatcga ggaagttcgg gaagtttttt cagcacttct
     2581 ataaagcgtt ctttttgcag attccaatat gtatcatctg cctgatctgc tgtagaaaaa
     2641 tgggtaaata aaccttccca ttctaataca cgactttctt tgatgaatgc agctgcctct
     2701 tttacttctt caggtgtaca aaatccgata cgacccatcc cagtgtctgc ttttagatga
     2761 atcgaaagag gcgtctctag cttacttttc tctaaaacag cagctgctgc ttgcaaccat
     2821 tcccgtgtac ctgcagttac tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa
     2881 ggaaccatat tcaagataag gatgggctga gtaaatccag cttctctcaa ctcgactcct
     2941 tcatctaaat ttgaaacaca aaagcccgaa gctccacctg caactgctgc ctcagctgtt
     3001 tcaatagctc catgaccgta tccgttcgct tttactactg caaacaattc ttttccttgc
     3061 ggaagtcttg caacttcatt tgctacattt tcagttatag cttttttatg tatcactgct
     3121 ttcgttgggc ggtgccaagc gacaaccatc gtttttcctt ctttctagtc ttccaaaata
     3181 atttgtgcaa aagctgtttc atttgtatga gtaatactga cccatacatt gccttcatga
     3241 ggtgaacgcg tcacaacagg ctgaccattt tcatttttta aaatctcgac atcttgaaag
     3301 gtaacttttc cgatcccagt tccccaagct ttagagaaag cctctttgca agcgtaacgc
     3361 ccgcctaaaa attctacttg gcgatgaaaa ggcaaggact ggaatagttt catttcatca
     3421 gaggtcaaga ttcgtgcaat gaattttggt ttttcttcga tcagccgagt gatcctagga
     3481 agttcgactg catcgatccc aattcctttt atcatatgct tttctctttt caataaattt
     3541 agaacactct cattctacca aattataacc attttgccac aaataagtcc acaaataaca
     3601 aaaatgaaag tttctcttaa aaaagagaaa ctttcatttt atgtttaatc gttatttgaa
     3661 cggattacaa atccgcgttt tttatcattg tgacgtttac cgccatcttt ttggctgtta
     3721 ttatttttgc tataaccgcc ttttgatttg tttccacgat aattaccgcc gtcacgacga
     3781 ttacggttgt ttccgccacc acgtttgcca tttttattgt atcctttttt ctgcattggc
     3841 aatgggcgtt ctggtgtgat cttcactggc actgcatcag ctggatcttt tgctgtcgtt
     3901 tttagcaaaa gtgcaactaa atcttgtgca gaatattctt ctaataattt atctgctgtt
     3961 tgcaggtatt tatctagacc attttctgca agttttgttt cgatttgttc aactgcagca
     4021 cctaattgac ctttgaatgc ttctttttcg gttggtggac gtaaagtcgt catccgtttt
     4081 ttcgtcaagt tttcaatcac atgcaagtag tccatttcat ttggtgtaac gaaagtcaca
     4141 gacataccgc cttttccggc acggcctgta cgtccgatac ggtgaacgta gctttctgga
     4201 tcttgaggga tatcatagtt gtacacatga gtgacacctg aaatatctaa tccacgtgca
     4261 gcaacgtctg tcgctacaag gatatctaaa tgaccgctct taaatgaacg caatacgctc
     4321 attcgttttt gttgagacaa atcgccatgg attccttctg cacggtaacc gcgtgcttct
     4381 aatccgcgtg ccaattcgtc tacacggcgt tttgtacgtc cgaagacgat tgttaattca
     4441 ggtgtttgaa catcaaacaa gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg
     4501 acatagtatt gatcgatcaa gtcagctgtc atttctttcg ctttgatctt cacatgatgt
     4561 ggatttttca tgaatttaac gccaatattc ttgatagctg gcggcatcgt tgctgagaaa
     4621 agtaatgttt gtctttgatc aggaacttgt gaaatgattt tttcgatatc ttccaagaag
     4681 cccatattca acatttcatc tgcttcatcc aaaactaatg tttgaacggt tccaagtttc
     4741 aatgtgtggc ggttaatgtg gtcaagcata cgtcctggtg tacccacaac gatatgtgga
     4801 cgatctttca agccacggat ctgacggcca atatctgctc cgccgtaaac ggcttgtaca
     4861 cgtacttttt tgtcgcgccc taaacgatat aattcttctt gtgtttggat agctaattca
     4921 cgtgttggtg cgataactaa accttgtaat tcgtgacgat ctggatcgat tttttctaac
     4981 attggtaatc caaatgcagc tgttttccct gtacctgtct gtgcttgtcc aataacgtct
     5041 tttccagcta atgctagtgg aatcgtttct gcttggatag gcgttgcttc ttcaaaacca
     5101 gcgcgttcga ctgatttcaa caattctggt gataattcta gttctttaaa tttcaaatga
     5161 atcctcctat atggttgtta actgaggatg tagctttttt gctaccccgt cgatttgttc
     5221 ggtcaaagtg tattgtttct ttttgacagt ggccacgcac gtttttatcc attcttagcc
     5281 cctgtggcac caccgaaaaa tcactttgcg aagtttaggc attagaaaag ccaaacgggt
     5341 cgcgttcagc tttatcatta ttatgacatc tggcttcaac aagttgtttc tatggcaaaa
     5401 ccagtccaat ttctcttcat ctttcggcaa taggttattc taacatagtt tttcgttttc
     5461 tgcaagttta cagattttta tttcatttgc tgaagttgtt ctaccacttc aaataagccc
     5521 atgctgttgc tgccttttag cataatacta tctgttgctt caagattttg tttgatagca
     5581 tcaatcaatt tattcttttc tttttccaaa tagaacacag gtagttgggg gtatttttgg
     5641 ttcaattctt cttttagtgc atgcatttgt tccccataaa ggaaaatcat ggcgaatttc
     5701 tcaggatcga tatgttcact catttgcgca tgcatattta aagactcttt ccctaattcc
     5761 agcatatctg ctaacacagc gattctgcgt ccttttgttg gcagattttt aaaagtatct
     5821 aataccagtc ccatggctgt tggatttgca ttgtagacat cgctcaaaat agctgcacca
     5881 tttcctgccg ttagccattc ggttctgttt tgagtgatag aaacatgggc gagcccttgg
     5941 cgtatctcag gtatggacag tccaaagaag cgcccgatcg tatacgcaat caaagcattc
     6001 gttacattat aacttccagg cagcgggatc gtaaatgttt ctccttctac tacaaaatcg
     6061 gtttgtgctt ttccttttgc tgtcacttcc gcatgaacat cgccatttcc aataccgaat
     6121 gttgtgactg tttgtactaa tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc
     6181 acaacgagta atccgtctga tacaagtccg tcagtgattt ccattttggc tttagcgatt
     6241 ccttctcgcg acccaagatt ttctatgtgt gcttcaccga tcattgtgat tgctgcagct
     6301 tttggttgag ccataagtga aagttccgtg atttcattgg cgtgatccat gcccatttcc
     6361 aaaacgatct tttctgtatc ttggggcatg tgcaaaatcg tgtaaggaag accaatatta
     6421 ttgttgtaat ttccttgcgt cttatatgtt ttgtattttt cagacaagac agacgcggtt
     6481 agatctttcg tggtggtttt accattgctt cctgtaattg caacgacttc tgcgcctact
     6541 ttttccaagt aataggttgc caattgctgc atggctttca aaggatctgc tacaggaata
     6601 actggaaaat tttttggagc ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg
     6661 tttgtgatcg cttgctggat aaattcatgt ccatctcgcg taccagcaag aggaacgaat
     6721 aaacttcctt cggttatctt ccggctgtca aattcgacat tggtaatctt tgtcagtgca
     6781 gagcattcgc tgcccacggc ttgtgcaatt tcttgtattg ttagattcat tgagatctct
     6841 cctaaaaaag gttgtgaaaa aagcgtttgg cctaaacggc gggttcgcat gactctcact
     6901 tccaagtaaa tttctctatt cgtatttaga agctagaatg aacgcggtta cgcaatcact
     6961 ccagcctctt ctttatttct ctttattcat ttttttcaaa aaaagattga cgctgatggt
     7021 atcgattcat tcctaactgg atcagttctt caatcaaatc accgtatttc aagcccatat
     7081 tttcccataa gagtgggtac atactgaact ccgtaaatcc tggcatagag tttaattcat
     7141 tcaggaataa ttcattttta tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac
     7201 ctaacatcgt gtaagctaac ttcgcgtact cttgcgcttt ttgataaact tcttctggca
     7261 cttcggctgg aatctgcatt tcgattttat tattgatata ttttgcttca taatcataga
     7321 atgctacgtc ttttacgact tcaccaggca aagtcgtccg aacgtcttca tttcctaata
     7381 cagcaacttc gatttcgcgc gcttcaattc cttgttcaac gattgctcga gaatcatact
     7441 gataggctgt tgctaaagca ttttgcagct cttctcggtt ttctgctttt gtaatgccga
     7501 cactagaacc catattcgcc ggtttgacaa acatcggata aagcaaagaa ccttcacatt
     7561 gatcaaatac ttttttagga ttttctttcc attgattctt aagtactggt acataaggaa
     7621 cttgcggcac accagcagct tgtaaaatat acttggtcat gattttatcc atggcacatg
     7681 cactggtcaa tacgcctgcg ccgacataag gcatattcaa tgtctctaag aagccttgga
     7741 tcgttccatc ttccccgttt ggcccatgta aaactggaaa aacgatggct ccttcttctt
     7801 tgatttcgcc cggattgatc acttttcctg taaagccttc ctctgtctgt ccacttgggt
     7861 cccatgaaag atgcaagaca tctttgctgg caggtttttc tgttagtaat ggacctttga
     7921 cccattgtcc ttcttttgta ataaatacga gttgaacttg gtaataatta taataaatgg
     7981 catttaaaac tgaaaatgcg gaaagaatgg acacttcatg ctctgcgctg cgtccgccat
     8041 atagtaaagt aatcttcaaa agattgtcct cctgtattct aaatcgttca gtgatttact
     8101 ctcatatttt agcacaaaac aaaaataaac gaataactta tttataaaaa aacgagccag
     8161 cttctaaaag aacgagcgtc atttattttt tgtcagctag attatttgtc ccgtttttta
     8221 taaaaagtta tgataaagaa aaggagggat gatgatggcg caaacaaatc cacgcagccg
     8281 cttggcagaa atcattcgag tgttcatcaa ttacaatgta gtgccaaact ttgtacagca
     8341 aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta ggccccacct tcatcaagat
     8401 aggacagatg ctttctgttc gagaagattt gctttcttct gccttcactc aaacattcaa
     8461 aacattacaa gatagtgtac ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac
     8521 agaactttct ctttcattat cagatatatt cgatgatttt tcaaaatcgc cttttgcctc
     8581 tgcttctatg gggcaggctc atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa
     8641 gatccaacac cctaacatcg ccgaagagat ccgacttgat cttcaactat ttgaacgagc
     8701 gatccctttg atcaaataca ttcctgaaac tagcgtagtt gatctaaaag gggtactcca
     8761 agaagtaaaa cgttccttga tcaatgaaat ggacttctta aaagaaagtc aaaatggcga
     8821 acaattctat caaaagaaca atggctggaa agagatccgc agccccaaga tttatgacgc
     8881 tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt
     8941 aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca agcagctgaa
     9001 acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa caggtgtttg atgatggttt
     9061 tttccatgct gatcctcatc cagggaatct gctattccat gtactaacaa aagaagaaca
     9121 gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt
     9181 tcgagcctcc acttctgcag aagaccctgt tgcgccatat accatcaact acatcgattt
     9241 tggtatgatg gggcatcttt ctgcaggact taggcaaaaa ctcacacaag ccgttttagc
     9301 gctgtatacg aaagatgctt atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga
     9361 aggctcgttt gatgaaagtc ggttccacca agaactgaca agttttcttg aacagtatta
     9421 tgattctcct attgatgaaa tcaacttgca agaagtattc acacatgtcg tgacgatttg
     9481 tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag catttgggac
     9541 attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc
     9601 ttttgctcaa cattattttc tcactcatct ggatgtagaa gatacattga aacaaagcgg
     9661 attagactta ttggaaggga tgaaggctgc gcctaagatc cctcaacagc tccaccatct
     9721 attagaaatg tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa
     9781 attattgagc aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc
     9841 attgatcgta gggtcctccc tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt
     9901 ttccttatta gggatattta cgtatgctat tgccgcattc gtcattattt ttttggcaat
     9961 cgatgcattg atccaaatgt ataagaagag gaaaaagtag aaaaaggttg tgacagaaag
    10021 aatcagcttc aagaaataag aaggggtttt acataaattg tttctcaaat ttttgtgaaa
    10081 attcggctta tttttcgaag aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa
    10141 gctgttctgc acccagtaat aagaacaatc aaaaagggaa catggaacac atttatcctg
    10201 tttccctgtt ccctttttga tgtattcgag cataaataaa ataccttcgt tacctactat
    10261 cttctgtact tggtaaacct tagtcaattg ccttttttaa cgcttgacga aaagacatat
    10321 gatctttctc cgtaaagggt tttggaccct ttgtccttat gccagctttt cttaactttc
    10381 cactatgata acgttgttct agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag
    10441 agtgatggaa cactcgcaca cccttcggca atacaagtga agctaaacca taatcttgcg
    10501 tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt ataatccgca ctatcagctc
    10561 cgcggtctac gtagacgatt tggacatgat tagcgtattc tttggtcgta taatggtcca
    10621 cactggtcac gataaccacg tcaagaccat aggattctcc taattttatg acatcttctt
    10681 ttactggaga accatctcca tcaatgaata gcctcattct ttttcgcctt cttccataaa
    10741 tgataaacag ttactaatcc gctcagtaaa agaccaacat tgatccattt tgccaagata
    10801 agcagttgta cactttctat cggcactgat tcatggttca gcgtgatcaa taatagatgg
    10861 attggctctc ccatttcttt tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg
    10921 atcacactcc ctttattttg agtagtatac ttgacaacga tccggcgatc cattggaata
    10981 gctgtatcag tttgataata ttccttattt agccagctgc ctgttactac gtgccagaaa
    11041 ctaagcagaa agacacctat cgcaaaacca tttggaatca agaggaccaa taaagaagaa
    11101 ctcagtccaa gcaattgcat agccaatgga gaccatgtta aacgtttcaa aatccatggt
    11161 ccgactattt ttgctgcaat caagcctgct gcataaggca aatacataaa aatcgaaagt
    11221 ttttctaatc cgtacagtat gccaacataa aaactgctaa ataaaaaaag gaaattccct
    11281 agcattccat ttagaaaagt aaacagattt agccagcttg gcaacttcca ttccttcttc
    11341 tggtgtccta aaacaaacac gaacaagatg aacagcagca agaaaccata aatagcaaga
    11401 tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca aaagaaacaa taagccaaag
    11461 aatatcaaaa ataaaacaag ctctcgcgta gctaccagat ttcttttgat gtctttgaaa
    11521 tccagttcgt aacgcggata atgactaacc gtatggtagg ccatgacgta aaaaagtgta
    11581 taaatagtaa agcttgctgc aatctgagtc ggtgcaggca actcaatcgc ttgatacagg
    11641 agaaatagcc agatagctgc aaataagtac tttttcccat ccatattgat aaaaccttgc
    11701 gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa gccatgctgc actcagccct
    11761 agcaaaacac ttgaaaaata aaataaagga aaataaaata cgcctaatat tccaagcaat
    11821 gaaccagcac caccaatcag cagagcgatc atcagcaacg tgtaactatc tagaccaaat
    11881 cgtagactgt tgactaaaaa tagtccagtc atccgaaagg cataaaacag cgtaaatggc
    11941 aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa ataaaagata aggaataaaa
    12001 ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg
    12061 agtttttcga acatgacaat ggctccttaa acagtcttat aactaatctg ctcttcgatt
    12121 ttagcattaa aggggaaata aaccaatttt acagaatata ttcttgtaaa aaaaaataca
    12181 gaaagaagca taagcttctt tctgcatttt tacggtactt gattactcaa gcagacaagc
    12241 ccttctccac aaaagtcttg cccgacaccc ctgcatccgg caggtgataa agatagaaat
    12301 caattatcct gtgcctccaa aatcaattag gagctctgta aaccagagtc cttctcgacc
    12361 cgtcgcataa atttacttta acacagttcc tatgtttatg cgagagcttt tttcatttta
    12421 taatcgacca tttgaaatcc ttgaccgata tctgaaataa gttcttctgt aatctcaaat
    12481 ccttcatgct tataaacctc gattgcttga tgattcccct gattcactcg caaaaagatc
    12541 tctctttcct ttgtttgcgc agcaagttct tcataccagc gaaaaactga agaagtcaat
    12601 ccttgaccac gtactttatt tagcaaatat aatttactta aataaatttt tccatttatc
    12661 tcttcataag cagtgtaacc gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca
    12721 ccttctgtca gttctttttg aatctgtttt tttgactgat aagttttcag catgtaatcc
    12781 acttgttttt gaccaatgat cggcacaaaa gcttccggcc atacttcttg tatgactgcc
    12841 atcagctcat ccagttcatt ttctgttact tttatttttt ccatacaccc acgtcctttt
    12901 ttacattata aatgaccttg cgcttgatgg aataaaaaga gcatgatcat gctatacaca
    12961 cccaccgacc atggtttcaa gaaaatcaca atctgcataa attccctaaa agataatttc
    13021 gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa agggaagcag caaggtaaca
    13081 actagtactt ttttaatatc tttcgttttt cttgtcagca attcatcaaa tttctgatac
    13141 ttttttggcg ataaaataag ctggacaaag gctacgccat agtaacggac aagcaaaaat
    13201 ccgattgctt ccccaatcac taggccaata caactatata aaagcccaaa tccattgcca
    13261 aacatcagca tgcccgctac tgaggagatc cctcccggca aaataggaat gatcacttgg
    13321 atcacttgca acagaacaaa tatcatcact gcatacttgc caaattgctg gataaacgct
    13381 tgcaaagacg tgctcgatcg aaaaatccca tgatgataag cataaagaat caacacaaag
    13441 aaaaaaaaca gaccaataat gggcatgatc tgaagacatt tttgtaaaac tgatcctttt
    13501 ttagacatct atattcccca catctttttt catttattaa aactgcctcc t
//
LOCUS       DASJST010000074        13174 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJST010000074 DASJST010000000
VERSION     DASJST010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.074"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(53..1864)
                     /locus_tag="U4B05_001745"
     CDS             complement(53..1864)
                     /locus_tag="U4B05_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN2050884.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1861..2226)
                     /locus_tag="U4B05_001746"
     CDS             complement(1861..2226)
                     /locus_tag="U4B05_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN2050885.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2389..2784)
                     /locus_tag="U4B05_001747"
     CDS             complement(2389..2784)
                     /locus_tag="U4B05_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2050886.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2802..3764)
                     /locus_tag="U4B05_001748"
     CDS             complement(2802..3764)
                     /locus_tag="U4B05_001748"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN2050887.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3764..3976)
                     /locus_tag="U4B05_001749"
     CDS             complement(3764..3976)
                     /locus_tag="U4B05_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN2050888.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(3997..4695)
                     /locus_tag="U4B05_001750"
     CDS             complement(3997..4695)
                     /locus_tag="U4B05_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN2050889.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4715..5257)
                     /locus_tag="U4B05_001751"
     CDS             complement(4715..5257)
                     /locus_tag="U4B05_001751"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN2050890.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5389..6393)
                     /locus_tag="U4B05_001752"
     CDS             complement(5389..6393)
                     /locus_tag="U4B05_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2050891.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6390..7379)
                     /locus_tag="U4B05_001753"
     CDS             complement(6390..7379)
                     /locus_tag="U4B05_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2050892.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7376..8182)
                     /locus_tag="U4B05_001754"
     CDS             complement(7376..8182)
                     /locus_tag="U4B05_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2050893.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8348..9304
                     /locus_tag="U4B05_001755"
     CDS             8348..9304
                     /locus_tag="U4B05_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2050894.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9381..9899)
                     /locus_tag="U4B05_001756"
     CDS             complement(9381..9899)
                     /locus_tag="U4B05_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2050895.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9987..10136)
                     /gene="rpmG"
                     /locus_tag="U4B05_001757"
     CDS             complement(9987..10136)
                     /gene="rpmG"
                     /locus_tag="U4B05_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2050896.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10364..10810
                     /locus_tag="U4B05_001758"
     CDS             10364..10810
                     /locus_tag="U4B05_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2050897.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11005..12900)
                     /gene="asnB"
                     /locus_tag="U4B05_001759"
     CDS             complement(11005..12900)
                     /gene="asnB"
                     /locus_tag="U4B05_001759"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN2050898.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4094 a         2804 c         2238 g         4038 t
ORIGIN      
        1 aaaaatacgc atgaagactt tctaaaaaac agataagtca tcatgcgtat ttttatttgt
       61 cagtcaatat aagttgtgct attttatctg cctgtctcag attccatgga tctgtataag
      121 gtctagctac tgctgcagtt agccaatcca caccttcaac aggtattcca tagcaataaa
      181 agtcagactg gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta
      241 tagcttctga atgataactt cctgtattat ctgaaaatga aaaagggcga acaagtttgt
      301 tttccttcaa attttttatg acaggattac ttgaacgaga aaagcttgtt tgtggcaatc
      361 gagcttcaat caaatagcga ctgctgattt ctttatcttg tagagaaata acatagcggt
      421 tgtgcttttg ctgaatatca aatggcggtt cgatcaaatg aaaaataccc gcatctatca
      481 gtgctgctaa ttctttcgtc cgaatgattg gcggaccgat cgttaaaaag ctatttagat
      541 gagtaaacca tccccatagt atttctttgt attccttagc agaaaaaacg tgccattcaa
      601 tagcctgccg gattggatca cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc
      661 ctgtgcaatt gcctttttcc gcttctttta tctgcccttc aataaatatt cggctagctt
      721 ctgaaaaggg ctcattctga taggcaagag gagtttctaa atacgaccaa tcccatctat
      781 aagggattag tgctggatgc tttccgagac actcctctga agagagggag agaaaatcat
      841 gctcaaaatt tttctttgga atcggcaagt gatattcttc gatgattttc ttgtaataaa
      901 acaactcaac gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa aatcccgact
      961 gatgccattg ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt
     1021 gcttttgatt tcttcctcga gggtaataag ggagccccct tcttgatcca ctgtatacaa
     1081 tcggttcctt gcctgatgga tgatagacca gttttccctt ttcgttctcg aagcgccctc
     1141 ctcttgatac tgtaaacaat ccgacataat caaaaaaact taagcctaga cctcttaaaa
     1201 agacattttc tttaggcggt atcttcgaaa gatctacatc tgcaggatta gaaggaagct
     1261 gatacagtaa aacatttttc tttgcgtagt ctacccacac tctttcttct tctgtctcat
     1321 cattttccca gtgaccgctt gctaaaacaa gctgatcgat agaatagctt tgctgatctg
     1381 tctgtaaaag ataaccatct tttcttttgc ttactttttt tactaaagaa taaacaaact
     1441 gaatagaagc tggaaattct tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt
     1501 ataaacatcg agtagattgc tgatcttttt ccagacgatt gatttcttct ataattagtt
     1561 gcttattttt ggttaccgtc tgttcgatat aatctgtagc aggtccttta ctccattgaa
     1621 acaagttggg tccctgtgtt accaatcctc cactggacaa tgtttcatct gtaaaaagcg
     1681 tcacttgttg agaaacagaa ttcattaata actccggtgg ctgattcaac cgccaaactc
     1741 gacctccagg accattggga tcgaaaagaa aaagttcaag agaatgtctg tcctgattat
     1801 tatggagaat cctttctgct acacttaatc ctctaggtcc ggcaccaatg atccctattt
     1861 tcatttgcat gcctccattt cgataaattt tttcaataaa acagcatggt tctgatgttc
     1921 attttttgca ccataaacaa aagtaatgac tggatgttct ttcataagtt gactgagttt
     1981 aaaaatagct tgcttctttt cttcttcgaa aatcaattct tgctggtatt tctttgcaaa
     2041 tgaagcaaat cgttctggta catggtcaaa agttttgcgt gcttcattac taggtgcaat
     2101 ttctttcaac cacagatcta actgcgcttt ttcttttgaa attcctcgtg gccagaatct
     2161 atccacgagt attctatagc catcctcttt ttcagcatca aggtaaacac gttttaattt
     2221 gatcatagtt gtttccttct ttctaccttt tcgcttagct tatatatgta gataatagca
     2281 caaaataaaa aaaatgaacg gcacaactat ccattgcgcc gttcatgtga tcttggtcaa
     2341 aatctaatga ttgattcggt ttttaaagct gtcatacaat catctgtttt atactttttt
     2401 acttttgatt tttccagacc atccgtcata tccaccctta agtaaataaa tctcttgata
     2461 acctgcttta cgtaatttat ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat
     2521 atagacaggc tggtcttttc gaatagatcc taatgaatct ttcaaaacag tgtaagggat
     2581 gtttcttgca cctaaaatat gcccagcatc aaacacatct ttttcgcgaa catcgattac
     2641 ttgtgctttt ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt
     2701 ggccattact cgtagataga cttcccaaag gatgattgca agcacaatca atagcaaaat
     2761 gatattaatt acccataaaa ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct
     2821 aatgaggcag caccaatgac accagcttca ttaccaagct ctgctaattt gatttttgtt
     2881 gattctctta cttgcgggaa agtaaattct ttaaaatatt tctctacacg gctacgtaaa
     2941 aattctcctg ctgccgatac tcctccacct aacacgatac ttgacggatt taatgtattc
     3001 cccaagtttc cgcaagctaa acctaagtag aagcatacgc gatcaacaac catcaatgca
     3061 aacggatcgt cttcttgagc catttcaaag atatctttac ttgttacttc ttctccgttg
     3121 tctagcattg ctttcagttt tgaatcccct gcgtattctt ctgccaagaa acgacctagg
     3181 cgtaccacac ctgtagcgct agatacagtt tctaaacatc cgcgtttacc gcaagtacat
     3241 tcgaaccctt caggatccac agtgatatgt ccgatttctc ctccagctcc gctcacgccg
     3301 tgaatcaaat ttccgccagc aacgatacct ccgccaacac ctgttcctag agtaacgaat
     3361 acaacgtctg gatcgttttc tccagcacct ttccatcgtt cacctaaagc agcaacattc
     3421 gcgtcattat cgatagcgaa tttgattcct gttcctgcct cgatcagttt tttcacaaat
     3481 tgaacttctg tccagtttaa gttatacgca ccaataaccg ttccagcttc gctgtccacg
     3541 cttcctgggg tacccatgcc gattccgata aagtcttcgg ctttcatatt atataaattc
     3601 aaacgatgat tgattgattc aatgatttca ggaacgatat gcttaccatc atccaaaata
     3661 ttggtatcga tgctccattt ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt
     3721 gctgttgttc cacctaaatc aatcccaata atcttcttgt ccattacaaa tttcctttct
     3781 ttcggctttc ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat
     3841 catgatcaat caaacggcta tcatacagat tacgcaattc gatagtcatt aattcaatgt
     3901 catagatgcg tgcacccaca tagataaaga tgccaaattt tttgaacagc tgctgtacat
     3961 catataagct ctccatcttt aagcacttcc ttcatattat acaagtaaac catatttttt
     4021 taaacccagt aaaatacata ttacaaaaag aaatacaaaa actagtgtag aaagaatcct
     4081 ttggtgtatc gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa
     4141 tccgccaatt agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa
     4201 aacaaatgtc aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt
     4261 atgtttaaaa tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact
     4321 tgcaccacca gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat
     4381 cccgctgaac atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg
     4441 ccccataaaa tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa
     4501 gattggtgtc actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc
     4561 ggcataataa gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg
     4621 tatccctaaa aaagcatagg tccataacgg tcgtcttaac catgcacgca attttatttg
     4681 ctgttgataa ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct
     4741 tctgatcaaa gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac
     4801 atgttttttt atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc
     4861 gataaccggc tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca
     4921 aatagagctt ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact
     4981 gttcatccat ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt
     5041 tcttttgttc tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg
     5101 ataaagtcgt ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat
     5161 agattcgctg ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga
     5221 ttcctttccg gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat
     5281 aaaatggtat aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag
     5341 aataaaaaga agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag
     5401 cgcgtgtttt tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat
     5461 caaaattcct gtaggtactt ccccacttgg tagaatcact ctagcaacca tatctgcact
     5521 aagcagaaaa atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg
     5581 tacgactagc ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac
     5641 cgctacacta ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc
     5701 cacgttaagg cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg
     5761 tgcataaagt cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata
     5821 aggccagctc gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc
     5881 cttagataat tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat
     5941 accggacaat aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata
     6001 gacacagcaa gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag
     6061 cgaatagtgc gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat
     6121 gcccaatata ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga
     6181 aacgccaaga caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct
     6241 aacaatcagc gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag
     6301 gtaaataaaa ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag
     6361 caagaaatag aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt
     6421 aaaaacggaa cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg
     6481 atcatcctcg ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca
     6541 ggtagaacat aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc
     6601 ccaataaatg aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga
     6661 cagaaaacaa tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca
     6721 ctcaaatgaa tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata
     6781 tataaaggaa acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac
     6841 caaaaagcaa gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta
     6901 aaaaaagaac taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc
     6961 gaaaactgca aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt
     7021 agaaacacag aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac
     7081 gaagcacccg cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcactcct
     7141 tgcatcaatg caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga
     7201 ggcaaacgaa tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac
     7261 gcctgccaaa aatctgcttt ttccacagtc gctgcgccat ttgaaagagc aacaaaaaaa
     7321 ataatcaaaa gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata
     7381 attgatcctc ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc
     7441 ttggaagcgt gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga
     7501 tcacttcttc aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat
     7561 ctgaaaaacg tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca
     7621 tttgattgat ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg
     7681 ttgtcggttc atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga
     7741 cacgctgggt ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac
     7801 ctgtagcatc caacgcccac tcaattgctt catgatcttg cgtgtccatg ccactgaaca
     7861 gtttttgata agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt
     7921 gttcactagt ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag
     7981 agatatcaga gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac
     8041 ttttcaataa agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt
     8101 caggaatcgt aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt
     8161 cagctgctct taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg
     8221 acgctgtttc aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg
     8281 acagcgttcg aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag
     8341 gaaagaaatg aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg
     8401 agtactgagc gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac
     8461 gacttcatcc aatagtactc atacagtcac agatacactt ggacataaag tagagattcc
     8521 aaacgcgccc aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga
     8581 aaaacctatc gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga
     8641 tttaaaggat gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga
     8701 gcctgatttg ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata
     8761 tagcaaaatc gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca
     8821 actaaaagat gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga
     8881 ttatcaaaaa tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac
     8941 agctgcagtg ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag
     9001 tggaagaatc gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc
     9061 caaaaaagcc acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc
     9121 tgactacatc atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg
     9181 gaagaatcta aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg
     9241 gctatataac ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa
     9301 ttgattttaa ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta
     9361 acaaactaga cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc
     9421 catttttgaa aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct
     9481 ataagggcga agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta
     9541 tcgatcaatg ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta
     9601 ccacccgaaa catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc
     9661 gaatgcatta cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg
     9721 atatggcgat aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact
     9781 aaaattcgat ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt
     9841 aacaaaccat ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata
     9901 ttatccgctc cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca
     9961 gcaaggaatt gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt
    10021 tcgcgtgggc aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt
    10081 gttagatagt tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt
    10141 ttccctccca tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat
    10201 gattttttct agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata
    10261 aatacaacgg ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac
    10321 ttgtttatat agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa
    10381 ggactttcct gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat
    10441 cgcaaatgca ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga
    10501 tcaagcaaaa aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga
    10561 aaaaaatcca agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga
    10621 ttcagatgtg atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa
    10681 aaaggcaagc aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc
    10741 acaaaacaaa gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat
    10801 cgattattga acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt
    10861 cgaaacaatg gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg
    10921 aagagctgtt ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc
    10981 ttttttattg atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg
    11041 aatcgtttca tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat
    11101 ttttctttgc tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc
    11161 ttgatcaaaa aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc
    11221 gtgttcttct ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga
    11281 ccattcttcc ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg
    11341 attcaataaa aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa
    11401 aggtactcgg acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc
    11461 ttttggcatc cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc
    11521 ttctgttttc tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt
    11581 tggtgttaac acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata
    11641 ttcttttgca ggagcaagtg attcatataa atggatccgt ccatggaagt ttttcatttt
    11701 tccgatttta cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc
    11761 aatcacacgg atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag
    11821 ttcatccgcc ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa
    11881 aaagtacaaa ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa
    11941 agggaatgct ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa
    12001 tagttcggtt agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat
    12061 tgagaaagaa tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga
    12121 taagaaagaa ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt
    12181 ttcgtcgatt ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc
    12241 atcccaatat tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg
    12301 caaacgatag acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat
    12361 ataaggtttc aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact
    12421 tttgatttct gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat
    12481 cccaaagtgg tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc
    12541 aaacattccg cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag
    12601 gacttctgta tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa
    12661 aggctggtaa ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta
    12721 aataggctga gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac
    12781 atgtttatcg atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat
    12841 tgtattaata attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat
    12901 ttaactttca catcctaata taaaattact gtttatttca ttcatatgca tcttatcata
    12961 gaataaatag cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg
    13021 aaagtttctg tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt
    13081 tgaggctacc ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa
    13141 ccagcaaaaa aagacacgac ctaaagatcg tgtc
//
LOCUS       DASJST010000075        13085 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJST010000075 DASJST010000000
VERSION     DASJST010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.075"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..725
                     /locus_tag="U4B05_001760"
     CDS             <1..725
                     /locus_tag="U4B05_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050899.1"
                     /translation="LYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLFKNAKEELILNSD
                     YEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEEELLKQEIKELKG
                     IRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHKNILLVGGHVNFQ
                     NKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYRVMSEISNKDKIF
                     YLDNHVNMNLTIKRIYDLLSLI"
     gene            776..1147
                     /locus_tag="U4B05_001761"
     CDS             776..1147
                     /locus_tag="U4B05_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050900.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            1232..1432
                     /locus_tag="U4B05_001762"
     CDS             1232..1432
                     /locus_tag="U4B05_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050901.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            1566..2792
                     /locus_tag="U4B05_001763"
     CDS             1566..2792
                     /locus_tag="U4B05_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050902.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            3389..3784
                     /locus_tag="U4B05_001764"
                     /pseudo
     CDS             3389..3784
                     /locus_tag="U4B05_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3862..4901
                     /locus_tag="U4B05_001765"
                     /pseudo
     CDS             3862..4901
                     /locus_tag="U4B05_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            4914..5852
                     /locus_tag="U4B05_001766"
                     /pseudo
     CDS             4914..5852
                     /locus_tag="U4B05_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            5845..6192
                     /locus_tag="U4B05_001767"
     CDS             5845..6192
                     /locus_tag="U4B05_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050903.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            6507..7364
                     /locus_tag="U4B05_001768"
     CDS             6507..7364
                     /locus_tag="U4B05_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN2050904.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            7384..7944
                     /locus_tag="U4B05_001769"
     CDS             7384..7944
                     /locus_tag="U4B05_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEN2050905.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            8833..9600
                     /locus_tag="U4B05_001770"
     CDS             8833..9600
                     /locus_tag="U4B05_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEN2050906.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            9626..10171
                     /locus_tag="U4B05_001771"
     CDS             9626..10171
                     /locus_tag="U4B05_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050907.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            10172..10738
                     /locus_tag="U4B05_001772"
     CDS             10172..10738
                     /locus_tag="U4B05_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050908.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            10753..11001
                     /locus_tag="U4B05_001773"
     CDS             10753..11001
                     /locus_tag="U4B05_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050909.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            11020..11259
                     /locus_tag="U4B05_001774"
     CDS             11020..11259
                     /locus_tag="U4B05_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050910.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            11276..11860
                     /locus_tag="U4B05_001775"
     CDS             11276..11860
                     /locus_tag="U4B05_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050911.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            11967..12248
                     /locus_tag="U4B05_001776"
     CDS             11967..12248
                     /locus_tag="U4B05_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN2050912.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            12238..12579
                     /locus_tag="U4B05_001777"
     CDS             12238..12579
                     /locus_tag="U4B05_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2050913.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         4919 a         1774 c         2619 g         3773 t
ORIGIN      
        1 ctctatatgc aattgatcag aataccgagt accgacaaat tgactatttg ctagtttttt
       61 atagtttgtt atatcagttc gttttaactc ggttgtttaa aaatgctaag gaagaattga
      121 tcctgaattc tgattatgaa atagcaaaac aaattgcaag tattagaaaa gaaaacaaat
      181 ctgaagtaat caaattaaga aaagaattaa gtattgcgag taaagaaaac ggtcggttag
      241 aatctttagt caaatcggca aaaaaagaag aggaactttt gaagcaagag attaaagaat
      301 taaaaggaat cagaaaagaa cttgaacagc tgaggtttga aaataaagta ttaaagcaat
      361 tgcaggagca agaacataga caatcattaa atggtaaaac tgaagagcct tcactgtctg
      421 aaatgatagc ttatctaaaa cataaaaaca ttcttttagt gggaggacat gtaaattttc
      481 aaaataaatt aactgaacta ttaccaaata ttaaaatgtt tgatgtaagc gaaaataatg
      541 gaaagatact taaaaatttg aatacgtgtg atgcgatttt tctatataca gattacttga
      601 atcatggtct atattatcgt gttatgagtg aaatttctaa taaagataag atattttatt
      661 tggataatca tgtgaatatg aacttaacaa ttaaacgtat ttacgatctt ttatcactta
      721 tataaagaga gaagtgataa ataggacaca aaatttggaa agtaggtaag tcactatgag
      781 agaactaact ttatcttcta aggaattaga aaagaaaact gctttattaa acgaaatatt
      841 tagtgctgat tgtataatcc aaagtttaaa agaaagttca ttttcaaaac taacagatca
      901 ggaaaaaatt tcttatatgc taagtttaga taatctagga actaaatatg atgaatcagc
      961 aagtagcgat aagcttttga ttgaattagt atcaagtctt agtagttttg gaaaaatgaa
     1021 aagatttaat gtgatattag aaatttttgc ttggttaatc ccttcattta tttggttttt
     1081 ttatattttc atagttgaat ctgagttaat agccaaggtc tctcttggac tgttaattcc
     1141 gatataaata ggttcagcaa tttactttaa taacttgtat acacctattc aaataaaaaa
     1201 gcagttatta catgaaggat taagtggtaa tatgttaaaa agaattgcat gggataaaga
     1261 taataaaaca gatattgagc ttaaagaata taaatacaat tatttctcga gatgtataga
     1321 tagaaagtta ctgtttaaaa aagaaggatt agaatttagt tttgatgaag atgaatttga
     1381 aaaattagtg atggactggc gtaagaattt actggatacg gtacaattct aaaagcaatt
     1441 gaggtcgaaa ttccgatact tgaatcatga tctatattat cgtgttatga gtgaaatttc
     1501 taataaagat aagatatttc atttggacag tcatgtgaat atgagcttaa caattaaacg
     1561 tatttatgat cttttatatg tttataaaca ttaatgaggt gctaaaattg acaacaataa
     1621 ctatgacaaa aaaaatagaa gtatatgttg atggatcaca agaagaaagg gattcctttt
     1681 ttgatttttt ttggaaaaat agtagagaat atgtaaaagc gttgaatctg gtatataaac
     1741 agctaatgtt tgaagagttg ataaaagatg caattaaaga aatggatcaa ggttatgctg
     1801 agagagaaga aagtattcaa aatcggattg aaaagttagc atatactgat ccaatagatc
     1861 aaaaaaaact tgataaagca aaagctgaat tatctaaatt gagaaatgaa aaaaataaag
     1921 aagcgagaga aatattaacg caagcgatag ggttgaagag tcaaacgaga acacgagatg
     1981 tagcaaaaaa attagacttt gactttggag attttgttga ttccgcaaat atgaaagcta
     2041 gtaaagattt tggcaatgat tttatcggaa ttgtgaccgg acaaagaacg cctagaatat
     2101 ataaaaatag aaacaaatat ggattattac cttttagagg tcgagatatt aaaatccata
     2161 aagatgataa gttttatatt tatttaccta aaggctttag gtttaaagtg aatttaggta
     2221 gcaagccaag aaaagcaaga gatgttgctt cagttttaca acacattatg gatggcgatt
     2281 acaaattaaa tcaaagtaat cttatttttg atggaagaaa attatttttt aatctaacaa
     2341 tttcttttga aaaaagctat aatgaaatgg aattcaagaa aggtaatgaa ttgatcgtaa
     2401 actttgcaga tttgtgtagc ggtgaatgtt taattaatga taaatctgta atgtcttttg
     2461 gtgatagcca atatataaga aacttcaaaa gtaaaatttt agcaatgaaa tcaaaagaac
     2521 aaagccgagg agtcttttct aagggcggtc atggtcgaaa aagaaaatta gatcaagatc
     2581 gatgggaagc aataaagaaa agagaaagta attttgttaa aacttataat aatcaatgtt
     2641 ctgcaaaaat cgttaaaact gctattcgca ataaagctca aattattcgt atcattgaac
     2701 cagataaggc ttatggcgaa tgggcttact atcaattgaa agaacaacta gtaaacaaag
     2761 gaaaaagata tgggatagaa gtcagaagtt agtaaattaa acaatcgttg atttggcaac
     2821 gaaatagtga gggaaaatcc actcactgaa gggtaaactc cgtcaagttg tgtgaccaga
     2881 cggaaggacg aatgttgttg aggttttaga accatataaa acagcatgtt tctcaaattt
     2941 ttaggataag ggaaattgct agcagtttgt tttagaacca tataaaacag catgtttctc
     3001 aaatgagtac tttaaattaa ttgatggcgt tcctgcttta gaaccatata aaacaacatg
     3061 gatttaaaac aagaagtaag tcagttctcc ttttacttta gtaaggaaaa ataaagtatt
     3121 ctcaataaaa cttaaaataa gcaaaccttt gttttaaaat gattgtcaag ttaaaaaaat
     3181 ctgggatcat ataaaatagt atagttctca aatatgatta gaaaccctat taaagagcaa
     3241 tattaagcca acttacttaa atcgtttaat taagaacaat aatctctttt agtcagactt
     3301 ttagtctgac ttttttgtat ttccaaaatt tattctttac aaaaacgaac atacgtttgt
     3361 ataattactt gtgaatgggg gaataaaaat gtttgattac agtagagaac cggtgaatga
     3421 ctatttttta atcgacatga agtctttcta tgctagcgtg gaatgtattg aacgaaattt
     3481 agacccaatg actacaccgt taattgtcat gagtagatcg gacaacactg gttcaggtct
     3541 gactctagct tcttcaccta ctgcaaagaa aaaatatcat attactaatg ttagtagagc
     3601 tactgattta ccttatccat taccaaaaga acttcatatt gttccaccac gaatgaactt
     3661 atatataaaa aaaaatatgg aagttaatca gatttttaga aagtatgtcg cagatgaaga
     3721 tttattgatt tattcgattg atgaatcaat tttaaaagtg acaaaatcct taaatttatt
     3781 tactatataa attgagttaa attgtattcg tttttcctcc tataggcgta tactaaagaa
     3841 aacacttgag gagtggggaa catggaaaca gaacgccaaa tccgtgaatt ggaaaaagcg
     3901 atgaatacga cagacaaccg ccggttgtac gagcgtttcc tggcagtcaa gttggttctt
     3961 gaaggccaca cccggaaaga agccgggcac accatcggcc gcgacgagca tactgtcagc
     4021 cactatgtga aaaactactg ccataatgga atccaaggtt tagtctccaa aaaacaaagc
     4081 ggacgacctt cacggctgac agatgaacag gaagcggaac tggtccaaat cgtcgcctac
     4141 cacactccgg aggaagtagg tttcaaatcc cgtgccaatt ggacattggc gattgtgtgt
     4201 gacctgattc tccgggaatg gagttatgga tatacgcaaa aaggcgtggc ggacctcctt
     4261 caccggctag gactaagctg gacccgtccg acgtataccc tcaaaaaagc ggattccaag
     4321 aaacaacagg tatttttgga agaaaccttc ccgagcctaa aaaaaaattg ctgaatagcg
     4381 atattgacca cctcctattc gaggacgaaa ccatgatccg ggattaccaa gccattggct
     4441 gcacctggtt tctccgcggc caccagcgaa aaatccccac ttatggacaa cacaaagggg
     4501 taaaattgat aggcaccttg gattatgaaa ccggtcggat catttgtaca gaagaagggg
     4561 catacgacgc agcggccttc ctccgttttc tggagcggac gttgcgggaa taccccaccg
     4621 gaaaaatcgt gatggtattg gataatgccc ttatccacca cgccaagctg atccagtctt
     4681 ttcttgaaga aaacgctagg cgcctggcac tcatcttcct ccctccctac agccctcagt
     4741 tgaacctaat ggaaggcgtt tggaagtggc tcaaggaatc ggtcatcaac aatgtttttt
     4801 ttgaccacgt ccagaaaatc aaacagtcgg tccgcggttt cttagccgat gtaaacgagc
     4861 gtccccttga ggtgattgac cgactgtgcg tgcggatgta aagtatttaa ttcaacctat
     4921 atagttcagg aacaagaagc gagagaagaa gagaattagc taaaataatc aaagatgaga
     4981 ttcagaaaga aacaggtcta atagcaacag tcggaatagg agataaccca ttattagcta
     5041 aacttgcact ggacaatgaa gcgaagagta gtaaagaatt tatagctgag tggacttatg
     5101 agactgtacc tgaaaaaata tggaaaataa acgagatgac tgacttttgg ggcattggat
     5161 cacgcatgaa aaaaagattg aatcatatgg gaattatgag tatagaagat ttagcaaatt
     5221 ggaatcccta cacaattaaa agtcagttag gtgtaatcgg actccagtta tatttccata
     5281 ccaatggaat tgatcgtacg gatattgcta ttccaccaga accaaccaaa gaaaagtctt
     5341 atgggaattc acaagttcta ccaaaagatt atacgaggag agaagaagta gaactagtag
     5401 tcaaggagat ggcagagcaa gtagccattc gaattagaca acacaattgc aaaactggtt
     5461 gtgtacgctt agcaattggt acgtcaattt tggaagaaaa atcaggtttc tcgcaccaga
     5521 tgaaaatacc aattacagat aatacgaaag aattacaaaa ttattgtctt tttttattca
     5581 ataagtttta tgaggggcag gaagtacgac atataggagt aacttatagc agattagtat
     5641 acacagattc cctacaatta gatttattta gtgatccaag aaaaaagatt gctgaagaaa
     5701 atttggatag gattattgat aagattagac aaaaatatgg atttacagct attatacatg
     5761 cttcatctat gttagaaggt gcaagaagta ttacgagaag cactttaata ggaggacacg
     5821 caggaggatt aggaggtttg cataatgatt gaatataacg atagaggacg tttaaaatgg
     5881 tcgggatttt atttgtctga tcatactgaa agaatcaatg cagacaatgt acaacgtgct
     5941 aatcataatt tggcaaaaga acaaatgacg actgaagaga ttgcgaaaat actgagaact
     6001 gctgtcataa aaaatagacc cgtctgcatt caaaaagagg aacgaaacgc tgaaggttac
     6061 tatccagatg atgtagtagg aaaaattatt ggatatgacg agcttggtgt atttgtggaa
     6121 gaagaaaaag ttcactatga tgaaattagg aatgtttctt tctcggaatt attaaaatgg
     6181 agtcagatat gaacattatt aaaaaatacg aattcggtta tattacgtat gaacagctaa
     6241 tagaggaatt atggggatat ggtcaacaat taattaatga agtggggatt gattgctttt
     6301 gtttctatat tgaatccgga gcaggatacc ataagaacag atactatata tttccatatc
     6361 caccaaaata gtctcttaga tatttttaaa ttttctccaa atactttcaa ttattgtgaa
     6421 atatttccct ttaattaatc aaatttttta taaaagtaat atataaaata aattgcttga
     6481 agtattatat ttagatatca taatttatgg aagaaaaatt aattaaaaag gatgagatta
     6541 taatggggtt actggttccg agagctaata cttatactat acctttaacg aaacgccaat
     6601 taattgctaa aagatatcaa aggattacta gagccatcaa tagagagttt tggaattctg
     6661 aaagtgatac tgctcacagt ttatacgtag gttcttatgg gagaggaacg gcaattagta
     6721 ctagtgatat agatataata gttgaattac cgatggcaga atttgacaga ttcaaaaatt
     6781 atttatcaaa tggcccgtct aagttattac aagttataaa aaacgcattt caggaaatac
     6841 taccaaatag tgatattaga gctgatggac aagttgttaa aataaatttc catgatggaa
     6901 tcaaatttga aatagttcca gcttttaatg aaaaagatta ttggggtgag tcaaaagggt
     6961 ttatttatcc agattcaaac atgggaggga actggaaagc tacaaatccc aaaaaagagc
     7021 aagaagccat gaaattaaag aatacaaaaa gtaataattt attatatgct acgtgtaaac
     7081 attttcgaca tgtacgagat acagaattta cgagttatca tctttcagga atagtaattg
     7141 acagtttcgt atatgaggct atgggaaatt ggaaattcgt tgaaaataac agtgggggtc
     7201 aaaacattag ttctgtatct tatgagacag ctttattaga atactataac tcacataaag
     7261 ttatgggcgg tttaaattta tactctcctg gaagcaatca atttgttaat tctgatagta
     7321 gtatcatatg tttagaaaaa gtattaaaaa aaatagcttt ataaagtttt atggaggaac
     7381 aacatgggag acgaaaaaaa gcgcttggaa gttcaattac gtgaagcata tggacgagta
     7441 acgtatactt acaccactca tttaaagcaa gccgataata ttatttctga caataaaatt
     7501 ttaaggtgtc ttcaaattgg tttgtcagcc cttactactg gaggggtact tagtaccatt
     7561 attactgata atagtgagtt aacctttgtt gctggattat gttcaacaat ttctcttgga
     7621 ttaaacttat actttaagaa ttttgatctt gctgaagaag ctaaaaatca tcaaattgtt
     7681 gccgataaat tatggttgat cagagaagaa tacgtaagct tactaacaga tttaaatcgt
     7741 ttaagtgttg aagatattgt gaaaaaaaga gatcaattaa tgaacgaaac tcatgaagca
     7801 tataaaaatg cccctaaaac aaatagtaaa agctatgaat cagctcagaa agcattaaaa
     7861 tctgaagaag agcaattttt tacagaagag gaattggata agatgttacc agagcatctt
     7921 agaaaaaaga gttctaaata ttgaactctt tttttatgct taaaattctc ttgtaatttt
     7981 tctattcatc tatatttaag aagaaatatg atgatagaaa catctcctaa agccaccgca
     8041 agccgttcta agcatagctt agcccgacat tgctagatat gctcatctca ccatgaacgt
     8101 ttcctaaagc caccgcaagc cgtcctagcc attgcctagt ccgacatcgc tagattagtg
     8161 catctcgcca taaaagtttt ctaaagccac cgcaagccgt tctaagcata gcttagcccg
     8221 acattgctag atatgctcat ctcaccatga acgtttccta aagccaccgc aaaccgtcct
     8281 agccatcgcc tagtccgatt ttgcttgatc ggttccattt ctgtttattt agtgggtcaa
     8341 caccgtaatc ttaaatagcc atcgcctatt taagattact aggtctgctt tgaaggtaaa
     8401 aagtctttca ttcttcaaga cttttaggct ttcaagctac gcctgaaagc attgaaattc
     8461 atcgctcttt atttggtttt gccatttata attccttacg cccattatct ttttcgcctg
     8521 tcataccttg tcagaagtgt ttagagctta tctcatcttt ggattaatac aagtggcgta
     8581 aatttttttc taaacgcaag cgttacgaaa ataatttact taaacacttg tattaatccg
     8641 gcagataaga acgcattcta atcactcccg acaaggcaag acaggcgaaa aagataaata
     8701 aatgaacgaa aggaagaaac ataaaatgca aaaccaaata gcgatgaaga aaaaaccacc
     8761 tccctctaaa acaatccaaa caaaaaagaa ttttgaaaaa ccaaaaatca aaaacagaaa
     8821 gaagcgttca acatgagaga tagaatttta gaagttaatc aagaaatggt aaacaagtta
     8881 acaaatatca gtgggaacta tgaaaagtac gatggtactt tacttgataa ctatattttt
     8941 cacgatacac aaaatatcag aattggcaga agtaaaccga gagcgttcat tctcatcaaa
     9001 gagaaatatt tgaatgaatg gaatagcacc tatgaaatgg ttatgactga taactttgaa
     9061 aaggtattgg atttcagaaa tgctttgttg ggttctgata tgggaaatag cagtcttgaa
     9121 tctattgaag ttgaaaacaa gaccgagaat gaaggtgaag gattgattaa gaatgtcttg
     9181 aaaatcgaac aaaaatatat cagtgaagat agaagttttt atagagtcac aagctcggtt
     9241 ggtctagcaa actttgctac taacttaatt ggaaacaatg ctagtgaatc tttgctagtg
     9301 atgtgtctga atacaaagaa tgaggtcatt gcttatagcg aagttttcaa aggtggaatt
     9361 aatcaatcag tagcgatacc aagagagatt tttcaactag ctttgctaaa caattcagca
     9421 aaaatcgcag tatcacataa tcatccgagc ggatatgaat ttccaagtga aaatgacaaa
     9481 agttttacta cgaatttgaa aaacgcagga aaaacaattg gtattgaatt gttagaccat
     9541 atcatcgtaa caagtaaaaa cacaagctat tactcatttc aagaaaatgg ttctttataa
     9601 aaattagaga aaagaggaaa acaagatggg cgaaagaaca caagcgttaa ttacagtcaa
     9661 atatggtaac agcaaaacga aagcaagcgt gtcgattcat tatcagtggg ggtatggtcg
     9721 atatatgctg atggacgtat tgaatcaagg aatttattta atcaataaag catacgactt
     9781 acgagataaa tcaagagaag aattagcaaa aggaatcttt gatagaagta caggagttat
     9841 caattttgtc acctatgaat ctggagtgag tgcttatgat gaaacagggt ttggtgggcg
     9901 ctatgaacct tatcaagaaa cgattgaaga tgtgatgaag ttttcagata acaatgatgg
     9961 ctatgctcat ttagagatgg tgttcgatga aaatggattt agtctagatg ataaaaccag
    10021 attgcgattg tttgattttg agggtcaaca gataggacta aacaagtttt gtgaaagcgg
    10081 aaaagagttt gctacgaaag attttattaa gggatacaga ttattgctag aaaattatgg
    10141 aataaaccta gaagaagaga gcgtgaaata agtgtttgaa aaaagaaaag accagttccc
    10201 taatttagaa gcgaaaagtc aaaaagtgat tgatggtgaa atgattgaat tattccaaaa
    10261 aagttatgga accatagaaa ctaccgcaag ttgcaaatac tactttcaag gaaagagaaa
    10321 cacgtttaaa ggctttgccg attctgatga agaattggca aagaaaatca ttgattggct
    10381 tgtcagcagt gaacacgtag caagagaaga taccgttaga acaacgatta atggggaagt
    10441 cctaacaggt actagatgtg tttgttgtaa tcatgaattt gtagaagatg aaggttgcta
    10501 ccgaatcaat gaatatggtg attttatttg ccagagttgc cacgagatag aatttccaac
    10561 cgaaagaagt tggtttgaat acatgctaca agattatgag atggaaacag aaaatggtga
    10621 agtagttttt tgtaccaaag aggaattgag taagagaagc gatgatgaaa ttgaagattg
    10681 gtgtgtgtgg ttagtaggga cagatgattg cgttgcatat tacacaacag cagaatagga
    10741 gtgtgaatca gcatggaagg aatggatttt atagatcatg aagatttgat tgattttggt
    10801 tatacatgga aaggaatggt aggtattagc agaagtcttg ctaatgcctt ttacgaaaga
    10861 aattatgcag tctatgtcct ttatgatgac aatacagaaa gccttgttga tgaagagtat
    10921 aaattggatt tagagaatgt tttatatggg atagaaaaag aagatttagc aaaatatatt
    10981 ttttcatggt tgggtcaata aacgaaagag ggtagcaaca tgacaaaatt caaaagtaaa
    11041 ggtatgcaat ggtgttctta ttattatgtt gggaatgatg aagagctttt gagaaaagcc
    11101 aaacaaaaag gttatccaga taagatgtta aaacagattg gctattacat gccagaagag
    11161 agaatagaaa aagaatcttg taaggttctt tttgatagtg aacagctatt agttttgtta
    11221 actcaaaata aattgatttg tcaatttgaa aataattaat atggggtgaa gaaccatgaa
    11281 tgagaataag atggtattaa gtgaatacgc aagattgaga accattaatt ggttgaatgg
    11341 tatgcaaagt agtggcgctt ttgatggatt tgatgaggag aatatggact ttgaaagttt
    11401 atcgaatgag gatattagat attattttat cgaatggtat tttacagaac cagatgaaga
    11461 ggtggaagat gaaccagaat taattttaga acgtcaaaga attttattca gtgaacaaga
    11521 tagtaagagg ttgtatggtt tatatgactt acaactgaaa ggattaaaag cgcagtatgt
    11581 tgctgatagt tacgatgata tggtagaaca gggggcagat agggttgtca cttattcaga
    11641 agatgatgaa gaggaatggg aaaactattc agatgatgag atatgcttta atgatgggta
    11701 tatgcctatt gaaatcaccg aagaagaata tgataaagtg tcggatagcg atgagattgg
    11761 tatgtgtacg gttgtaagtg gattggatat agatgtccaa agagtagagg ttgcatacca
    11821 gaagatgaaa gaacgtataa aaagtaaagt agaatcttga tttgattgga ttgtttgcaa
    11881 agtagctaat taagagtata atataatcat aaatgtagtt aatggatata ataatgagta
    11941 tattatactc aaaagaggtg aacgaaatga aggtaaaaaa tccaaaagaa aaaagcagaa
    12001 ttcaagtcgg aatagacaag gatttaaaag aaaacgcaga aatgatattg gaagagctag
    12061 ggttaaatcc aacaacagca atcacaatcc tttataagca agtggtcgct agaggtgagt
    12121 ttccagtaga gataaagcta agcgaagagg aaaagcaagg aatcagattg caacagctaa
    12181 ctatggatat gccagttgat gttctagata cagatgaaaa gttagaagag tggttcaatg
    12241 aagcctaatg agatagtaac ggtctatgtt gcatttacag ataaaaatgg tggtaaaaga
    12301 agacctattt tggtggttag tgataaagaa gatagagtag aattttttgg aatcacaagt
    12361 caatatgaga ataaatcaga ccaaataaag agagtgtatt ttcctattgc agaatgggaa
    12421 aaagtaggtc taaagaagca atcatggatt gacgtagggt ctttaaaggc tattcccaaa
    12481 agccgagaga acatatcatt taaaaaggtt ggtatgctat cagctattga tacgaaaaag
    12541 ctaaataggt ttatcgagag attacaaaga aaagtctaac aaggcttttc tttttttgtt
    12601 tatatgtata ttaagatata cgtttatata aacgtattta tgtatattaa aatacacgta
    12661 aatatatacg tgattataaa aattaagaaa atattaagta ttgttaaata gtgataatta
    12721 gtaatatacg aattataaga tacattttat atttaatgta tgtaaataaa taaaatgact
    12781 gacggatatt gaatagtaat aaataacaat aattcagacg gatacagtga gtgaagtagc
    12841 ttgctacgaa cgaacaggca gaatgaaacg gtagtttcat tagagcaagt cgcttgcgac
    12901 gtagcgttac cactgatgta actggcaagt tacatagaga gagtttgctt gcaaacgaac
    12961 gttacccact tgaatagaaa acaaaaataa catattagtg aattttgaat aggagccaca
    13021 actcaaccaa ccgcttgcgg tgggtgagtc ccacggagga aaagacgaac gaacggtcgc
    13081 gtagc
//
LOCUS       DASJST010000076        12938 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJST010000076 DASJST010000000
VERSION     DASJST010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.076"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(369..995)
                     /locus_tag="U4B05_001778"
     CDS             complement(369..995)
                     /locus_tag="U4B05_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050914.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="U4B05_001779"
     CDS             complement(1290..1625)
                     /locus_tag="U4B05_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2050915.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="U4B05_001780"
     CDS             complement(1612..1896)
                     /locus_tag="U4B05_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2050916.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="U4B05_001781"
     CDS             complement(1952..3475)
                     /locus_tag="U4B05_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2050917.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="U4B05_001782"
     CDS             complement(3468..4643)
                     /locus_tag="U4B05_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2050918.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="U4B05_001783"
     CDS             complement(4647..4832)
                     /locus_tag="U4B05_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050919.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="U4B05_001784"
     CDS             complement(4798..6492)
                     /locus_tag="U4B05_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2050920.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="U4B05_001785"
     CDS             complement(6489..6962)
                     /locus_tag="U4B05_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN2050921.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="U4B05_001786"
     CDS             complement(7031..7186)
                     /locus_tag="U4B05_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEN2050922.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="U4B05_001787"
     CDS             complement(7320..7700)
                     /locus_tag="U4B05_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2050923.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="U4B05_001788"
     CDS             complement(7704..7919)
                     /locus_tag="U4B05_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050924.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="U4B05_001789"
     CDS             complement(7922..8329)
                     /locus_tag="U4B05_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEN2050925.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="U4B05_001790"
     CDS             complement(8595..10070)
                     /locus_tag="U4B05_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEN2050926.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="U4B05_001791"
     CDS             complement(10060..10908)
                     /locus_tag="U4B05_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEN2050927.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="U4B05_001792"
     CDS             complement(10945..11307)
                     /locus_tag="U4B05_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050928.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11308..11466)
                     /locus_tag="U4B05_001793"
     CDS             complement(11308..11466)
                     /locus_tag="U4B05_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050929.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11607..12231)
                     /locus_tag="U4B05_001794"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="U4B05_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="U4B05_001795"
     CDS             complement(12228..12539)
                     /locus_tag="U4B05_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050930.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="U4B05_001796"
     CDS             complement(12583..12876)
                     /locus_tag="U4B05_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050931.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
BASE COUNT         3547 a         2713 c         2041 g         4637 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctc
//
LOCUS       DASJST010000077        12927 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJST010000077 DASJST010000000
VERSION     DASJST010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.077"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..1982
                     /locus_tag="U4B05_001797"
     CDS             99..1982
                     /locus_tag="U4B05_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2050932.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2057..2341)
                     /locus_tag="U4B05_001798"
     CDS             complement(2057..2341)
                     /locus_tag="U4B05_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2050933.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2331..3065)
                     /locus_tag="U4B05_001799"
     CDS             complement(2331..3065)
                     /locus_tag="U4B05_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN2050934.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3194..3817
                     /locus_tag="U4B05_001800"
     CDS             3194..3817
                     /locus_tag="U4B05_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN2050935.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4009..5130
                     /locus_tag="U4B05_001801"
     CDS             4009..5130
                     /locus_tag="U4B05_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN2050936.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5137..8265
                     /locus_tag="U4B05_001802"
     CDS             5137..8265
                     /locus_tag="U4B05_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN2050937.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8284..11478
                     /locus_tag="U4B05_001803"
     CDS             8284..11478
                     /locus_tag="U4B05_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN2050938.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11624..12331)
                     /locus_tag="U4B05_001804"
     CDS             complement(11624..12331)
                     /locus_tag="U4B05_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2050939.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4438 a         2248 c         2689 g         3552 t
ORIGIN      
        1 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
       61 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
      121 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
      181 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
      241 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
      301 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
      361 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
      421 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
      481 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
      541 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
      601 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
      661 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
      721 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
      781 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
      841 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
      901 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
      961 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
     1021 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
     1081 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
     1141 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
     1201 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
     1261 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
     1321 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
     1381 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
     1441 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
     1501 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
     1561 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
     1621 aagatttgtt gaaagacact gttctgatcg gactaggaac gaatggctca ttctcggaag
     1681 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
     1741 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
     1801 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
     1861 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
     1921 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
     1981 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
     2041 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
     2101 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
     2161 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
     2221 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
     2281 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
     2341 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
     2401 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
     2461 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
     2521 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
     2581 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
     2641 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
     2701 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
     2761 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
     2821 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
     2881 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
     2941 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
     3001 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
     3061 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
     3121 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
     3181 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
     3241 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
     3301 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
     3361 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
     3421 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
     3481 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
     3541 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
     3601 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
     3661 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
     3721 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
     3781 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
     3841 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
     3901 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
     3961 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
     4021 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
     4081 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
     4141 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
     4201 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
     4261 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
     4321 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
     4381 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
     4441 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
     4501 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
     4561 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
     4621 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
     4681 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
     4741 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
     4801 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
     4861 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
     4921 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
     4981 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
     5041 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
     5101 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
     5161 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
     5221 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
     5281 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
     5341 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
     5401 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
     5461 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
     5521 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
     5581 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
     5641 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
     5701 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
     5761 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
     5821 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
     5881 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
     5941 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
     6001 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
     6061 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
     6121 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
     6181 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
     6241 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
     6301 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
     6361 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
     6421 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
     6481 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
     6541 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
     6601 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
     6661 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
     6721 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
     6781 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
     6841 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
     6901 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
     6961 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
     7021 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
     7081 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
     7141 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
     7201 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
     7261 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
     7321 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
     7381 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
     7441 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
     7501 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
     7561 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
     7621 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
     7681 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
     7741 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
     7801 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
     7861 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
     7921 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
     7981 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
     8041 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
     8101 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
     8161 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
     8221 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
     8281 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
     8341 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
     8401 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
     8461 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
     8521 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
     8581 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
     8641 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
     8701 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
     8761 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
     8821 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
     8881 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
     8941 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
     9001 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
     9061 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
     9121 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
     9181 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
     9241 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
     9301 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
     9361 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
     9421 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg cgaagtcatc
     9481 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
     9541 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
     9601 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
     9661 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
     9721 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
     9781 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
     9841 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
     9901 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
     9961 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    10021 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    10081 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    10141 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    10201 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    10261 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    10321 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    10381 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    10441 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    10501 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    10561 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    10621 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    10681 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    10741 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    10801 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    10861 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    10921 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    10981 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    11041 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    11101 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    11161 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    11221 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    11281 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    11341 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    11401 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    11461 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    11521 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    11581 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    11641 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    11701 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    11761 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    11821 ctttctcagc tagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    11881 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    11941 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    12001 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    12061 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    12121 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    12181 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    12241 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    12301 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    12361 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    12421 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    12481 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    12541 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    12601 taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta
    12661 tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat
    12721 aagcgttata ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa
    12781 taaaaaacat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag
    12841 tacagtttta tacgctgttt catatgttta gaaaatttga attagatttg aaaactctaa
    12901 tttttaaatg ataaaaaaag cgagaat
//
LOCUS       DASJST010000078        12792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.078, whole genome shotgun sequence.
ACCESSION   DASJST010000078 DASJST010000000
VERSION     DASJST010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.078"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            102..950
                     /locus_tag="U4B05_001805"
     CDS             102..950
                     /locus_tag="U4B05_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2050940.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1596..1802
                     /locus_tag="U4B05_001806"
     CDS             1596..1802
                     /locus_tag="U4B05_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN2050941.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(2004..3005)
                     /locus_tag="U4B05_001807"
     CDS             complement(2004..3005)
                     /locus_tag="U4B05_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2050942.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3010..4923)
                     /locus_tag="U4B05_001808"
     CDS             complement(3010..4923)
                     /locus_tag="U4B05_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2050943.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5091..5597)
                     /locus_tag="U4B05_001809"
     CDS             complement(5091..5597)
                     /locus_tag="U4B05_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2050944.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5757..6197
                     /locus_tag="U4B05_001810"
     CDS             5757..6197
                     /locus_tag="U4B05_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2050945.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6223..7380)
                     /locus_tag="U4B05_001811"
     CDS             complement(6223..7380)
                     /locus_tag="U4B05_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050946.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7383..7739)
                     /locus_tag="U4B05_001812"
     CDS             complement(7383..7739)
                     /locus_tag="U4B05_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050947.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8037..9011)
                     /gene="sgrA"
                     /locus_tag="U4B05_001813"
     CDS             complement(8037..9011)
                     /gene="sgrA"
                     /locus_tag="U4B05_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN2050948.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9207..10046)
                     /locus_tag="U4B05_001814"
     CDS             complement(9207..10046)
                     /locus_tag="U4B05_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2050949.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10231..11118
                     /locus_tag="U4B05_001815"
     CDS             10231..11118
                     /locus_tag="U4B05_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2050950.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11712..12406)
                     /locus_tag="U4B05_001816"
                     /pseudo
     CDS             complement(11712..12406)
                     /locus_tag="U4B05_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(12390..12788)
                     /locus_tag="U4B05_001817"
     CDS             complement(12390..12788)
                     /locus_tag="U4B05_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2050951.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3865 a         2510 c         1933 g         4484 t
ORIGIN      
        1 caaaaatatt ttttgtttga ttttttccta ctctccttgt atctttaaca ttaatgagaa
       61 cggaaaaaat gataatctta ttatgaaaat gaggaattct catgagcgtc tccataagac
      121 tacaaatcaa aacaatgtac aacgacttca gcccaaaaga acaggctatc gccgattata
      181 ttttagaaaa tccaagcaaa gtaagtcata gtcctattag caatttagca agtgaactgg
      241 gcattgctga ctctactttt tttcaattta caaaaaaact tggttataat ggatttaaag
      301 attttaaaat ggccatgttg atccaagaaa atgatctatc tgctatttct atccacgaaa
      361 atattcagaa aacagatact gagctcacta tggcgcaaaa ggtgttcgag tctagtattt
      421 caactttaaa tgacacaaaa aagttgttac aacaaaagga tctaaaaaaa gcggctacta
      481 ttatcaatga atctaaacgt gtctattttt tcggtgttgg cggttcagag attgtagcaa
      541 ctgacgctta tcataaattt ttgcgttccc ctatcgcaac ttctcacagc acggattacc
      601 atattcaatt gatggaagct tctttattga cggaaaaaga ctgtgctgtt ttgatttctc
      661 acacagggca atcaaaagaa acaatccata ttgctgaaac cgtaagaaaa actggagcaa
      721 aaacaatcgt tattactagc caagctaatt cttcccttgc taaacttggc gatgtcgttt
      781 ttatttctat ttctgaagaa acagaattcc gatctgaagc tttagcttcc cgtatttctc
      841 aactaagcat tttggactct ctatatgtga ttttgatgtt ttacaacgaa aaagaatcac
      901 gtcgttctat cgctaaagtt cgacatacta tttctgaaat caaagaatag caccataaca
      961 gaaaaagcga aaaatccata aaacaaaaaa agccactacc aaatatttgg tagtggcttt
     1021 tttggttctg caagggtggt acagaacatc aatagtcggt gggttagaac gtatgtcatc
     1081 gcatctttgg agaaggttgt attggtatta ttcgagttac ttgttagatg cgataacaaa
     1141 gttagtatac agtagtaaaa aatttatctt ttcttttttt tattattttt gtttcttatt
     1201 ttctaaaaaa ttaaaaaaaa ttttggtgaa ttaagtattt ccttgataac actggctttt
     1261 ggcctcattt taggttatca tttgtaattt tatttcacag ttgatatgat tgttatgtaa
     1321 aataaaaaag tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat
     1381 cttttctatt atattcccat attttctatt attggtctag acgtatgatg gtaaactttg
     1441 tacctatctg attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg
     1501 ctagatgatc attttattca catcctaaca aacttgtcac atttatagat tcttaatcta
     1561 tttatcatac aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc
     1621 tttttttcgc tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca
     1681 ataacaacgg aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc
     1741 ttcttgcctc cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct
     1801 gacttataaa atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc
     1861 gaataaaagg tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa
     1921 ccatgaaaag cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac
     1981 aacctctttt gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt
     2041 tcttgcgctt gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc
     2101 tccgcataat ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct
     2161 tccatttgtt tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa
     2221 cgatccgcat gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta
     2281 ttatcaaagt aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact
     2341 tgagtaagtg cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg
     2401 ttctccaaag tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac
     2461 aaatattctc cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa
     2521 tctgcttgat cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact
     2581 ggtgtaggat gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc
     2641 gtgtctgaac atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat
     2701 gcgcctccat gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag
     2761 atatccatat tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt
     2821 tgttcgattt tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg
     2881 tcaaattgtt ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat
     2941 acagtgacta aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat
     3001 ttcattcatt taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa
     3061 aaatccacct gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct
     3121 tttttttgca tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc
     3181 acttgattga tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc
     3241 ttgctttttt ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt
     3301 ctcattaaaa atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt
     3361 cttttgctct gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc
     3421 tttgagtaga cttttattgt tttcatccac atactttacg tttatctgat catggaacac
     3481 tactttcaaa aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt
     3541 tccatgccct gctccttcta ctaataaaag atctttatac cctcctgata actcatataa
     3601 tttttgcgcg ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg
     3661 caatgaatct gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg
     3721 atcaaaatag tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat
     3781 gatgtctaac tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt
     3841 aaaacgtagt tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt
     3901 gacaggaata tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata
     3961 aagtaaaatc ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc
     4021 atctgactca tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga
     4081 atcaacaaat aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat
     4141 cccgttattc caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg
     4201 atcgacataa tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg
     4261 atgaagttgt tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc
     4321 tgtacctcca agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag
     4381 ataagtagca atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag
     4441 ctcagtgact aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat
     4501 ttctgtcatc tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt
     4561 accgatatca tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc
     4621 gtcgtttgac atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt
     4681 cgtttcttga tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt
     4741 ttgtgaagta ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc
     4801 ccgagattcc gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt
     4861 acttacagca aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt
     4921 cactcctttt tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa
     4981 ataaatggtt tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc
     5041 agaatggata aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc
     5101 aaaaatgtca gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct
     5161 ttcataaaag gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct
     5221 aatccatgag caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat
     5281 tcgatcgaat gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa
     5341 ttcgcatggg tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc
     5401 tcccatggtt ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt
     5461 ttcccaatcg tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat
     5521 tcgtgtatcg gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg
     5581 tcggctgtgt ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt
     5641 atctagctag ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact
     5701 tttcgactat ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga
     5761 tgattaaatt gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta
     5821 tcgtccatga tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg
     5881 aatttatcca cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt
     5941 ctattagtta taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata
     6001 ttgagattcg tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa
     6061 aacttttgac tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa
     6121 agcaagagct caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc
     6181 tttttgcaat gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa
     6241 tttttccaat atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg
     6301 cgcccaatca ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc
     6361 atatgccttt ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga
     6421 tgcactatat tttattgggc gttcagtcat agataatatc cttttctttg cccataattc
     6481 ctgtgtttta ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa
     6541 aatatccctg actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt
     6601 attaagctct ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat
     6661 cactggaaat atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt
     6721 cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata
     6781 atttttttct gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga
     6841 cgattcaatc cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc
     6901 caaataggtg ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat
     6961 gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc
     7021 ttcttttgtt atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga
     7081 gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc
     7141 tatactacgc gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt
     7201 tatacttgat aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa
     7261 aaagtatcgg atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga
     7321 taaatgatag tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa
     7381 acttatataa ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa
     7441 tccagtaaaa tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa
     7501 aactgagaag aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat
     7561 tctggatacc tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg
     7621 gtgtagttta gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa
     7681 caatagttca aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa
     7741 gtacatactc ctttttatct attatttaga cttattccga cagctacatg aataacgtac
     7801 aaatagattc gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta
     7861 ttcttgctct tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta
     7921 aacaaaaata gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa
     7981 atattcactc aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat
     8041 ttcactcgat ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca
     8101 cccatccatg taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt
     8161 ctattatctg tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc
     8221 tgttttagca cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg
     8281 ataggtacca aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt
     8341 cctgtgctac tctcactact catgcttcct gtgctacttt cactactcgt actctccgta
     8401 ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt
     8461 tcgctgctcg tgttttctgt gctaatttca ctgctcgtac tctctgtact gctttcaccg
     8521 ctcgtgtttt ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca
     8581 actgtccaac tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca
     8641 atcaaatcaa ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt
     8701 attgtatact caataccagc ggtccattgt acgtctttta aataaacttc cccaataact
     8761 ttttctgaac ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca
     8821 ttcacgcttt tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca
     8881 atccgatcag ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta
     8941 ctgattccaa tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct
     9001 gttttcttca tttctatcac tctccatata attaccttct atttgataaa ggaattatat
     9061 aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt
     9121 attcgcttga atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc
     9181 gatatcagcc ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc
     9241 tttgttttat atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt
     9301 gatatagatg cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc
     9361 agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct
     9421 tgtgtccaat tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag
     9481 ataagtgcgc tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg
     9541 ttaggattca atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac
     9601 ggaccaaatc taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc
     9661 cccactgcaa taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa
     9721 tctaaccgat acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt
     9781 ccaacaacga gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa
     9841 tcttctattc tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct
     9901 aaatgtccga ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc
     9961 gcaagtgcat tattgacaag aattcctaaa acaaaaccaa caaagactat taaaacagat
    10021 ccaatcaacc gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc
    10081 cttttctttc ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt
    10141 tttacgaact aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc
    10201 ttctgttaga ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta
    10261 attatgtgcc tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa
    10321 acgattgcaa cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa
    10381 gttaaatcaa ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat
    10441 gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa
    10501 aaactcggtc caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat
    10561 aagaaactct ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta
    10621 acagataaag atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc
    10681 attctatttt cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa
    10741 aatttctcca agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga
    10801 aaaatgaatt ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt
    10861 aaactaaaaa acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag
    10921 caaagctatt atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa
    10981 tacaaatctg agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg
    11041 gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta
    11101 aaaaatatat tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt
    11161 gatttttgga aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg
    11221 gccttttttt atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat
    11281 ttaaaaaagt tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat
    11341 gcttccctat tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc
    11401 gactgaaaag ttattttaaa aaagactaaa aatatccctt ctggacttgt tcttcttatt
    11461 ctgttatact agagaaacac gaaagatatt tttatgcgac acttttcatt tgtttttatg
    11521 aaatttattc aaaactggcg taacttcaga gttacaagga tgtattagag gttagggaat
    11581 acatcgttta aatgagaaat aagtaatagt tagtaatagt cacccaaact ttaaattaat
    11641 gaatattgaa tcgtcagata gcctcctcga gttttcgaga aggctatcgt ttattttatg
    11701 gagaaattga ttcagaagta atcagcgacc ttttttagtt caccaatcgt ttctgcttgg
    11761 aaagtttgca cccaatcttc ttggaagccc ctcactgctt tatcaatcga gggcatctct
    11821 aatacttgat ctagtagctc tttacctaac gttatcgctg agctccagat cgaatggctg
    11881 tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac
    11941 gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca
    12001 tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta
    12061 attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg
    12121 cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta
    12181 tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga
    12241 taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc
    12301 cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac
    12361 aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca
    12421 ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta
    12481 tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg
    12541 atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac
    12601 atttgtttat caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat
    12661 tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt
    12721 attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag
    12781 atcgtcatct ct
//
LOCUS       DASJST010000079        12740 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJST010000079 DASJST010000000
VERSION     DASJST010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.079"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..542
                     /locus_tag="U4B05_001818"
     CDS             99..542
                     /locus_tag="U4B05_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2050952.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="U4B05_001819"
     CDS             complement(548..1732)
                     /locus_tag="U4B05_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN2050953.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="U4B05_001820"
     CDS             2000..2698
                     /locus_tag="U4B05_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2050954.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="U4B05_001821"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="U4B05_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN2050955.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="U4B05_001822"
     CDS             complement(3508..4041)
                     /locus_tag="U4B05_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050956.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="U4B05_001823"
     CDS             complement(4338..6719)
                     /locus_tag="U4B05_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2050957.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="U4B05_001824"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="U4B05_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN2050958.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="U4B05_001825"
     CDS             7420..7959
                     /locus_tag="U4B05_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN2050959.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="U4B05_001826"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="U4B05_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN2050960.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="U4B05_001827"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4B05_001827"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="U4B05_001828"
     CDS             9066..10232
                     /locus_tag="U4B05_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN2050961.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="U4B05_001829"
     CDS             10278..11774
                     /locus_tag="U4B05_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN2050962.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="U4B05_001830"
     CDS             11855..12538
                     /locus_tag="U4B05_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2050963.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4273 a         2230 c         2630 g         3607 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    12661 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    12721 gcaggagaac accgtttatt
//
LOCUS       DASJST010000080        12738 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJST010000080 DASJST010000000
VERSION     DASJST010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.080"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B05_001831"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B05_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2050964.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U4B05_001832"
     CDS             complement(323..892)
                     /locus_tag="U4B05_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN2050965.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U4B05_001833"
     CDS             1078..1980
                     /locus_tag="U4B05_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2050966.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B05_001834"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B05_001834"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN2050967.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B05_001835"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B05_001835"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN2050968.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="U4B05_001836"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="U4B05_001836"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN2050969.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="U4B05_001837"
     CDS             8548..8733
                     /locus_tag="U4B05_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN2050970.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="U4B05_001838"
     CDS             complement(8795..9991)
                     /locus_tag="U4B05_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2050971.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="U4B05_001839"
     CDS             complement(10161..11597)
                     /locus_tag="U4B05_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2050972.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="U4B05_001840"
     CDS             11890..12699
                     /locus_tag="U4B05_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2050973.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaatt
//
LOCUS       DASJST010000081        12687 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJST010000081 DASJST010000000
VERSION     DASJST010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.081"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..649)
                     /locus_tag="U4B05_001841"
     CDS             complement(287..649)
                     /locus_tag="U4B05_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050974.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="U4B05_001842"
     CDS             complement(723..1103)
                     /locus_tag="U4B05_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN2050975.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="U4B05_001843"
     CDS             1167..2360
                     /locus_tag="U4B05_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2050976.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="U4B05_001844"
     CDS             complement(2437..3021)
                     /locus_tag="U4B05_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2050977.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="U4B05_001845"
     CDS             3222..4220
                     /locus_tag="U4B05_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2050978.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="U4B05_001846"
     CDS             4332..4841
                     /locus_tag="U4B05_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN2050979.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="U4B05_001847"
     CDS             complement(4871..5530)
                     /locus_tag="U4B05_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN2050980.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="U4B05_001848"
     CDS             complement(5532..6296)
                     /locus_tag="U4B05_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN2050981.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="U4B05_001849"
     CDS             complement(6304..6981)
                     /locus_tag="U4B05_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN2050982.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="U4B05_001850"
     CDS             complement(7018..8403)
                     /locus_tag="U4B05_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2050983.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="U4B05_001851"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4B05_001851"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="U4B05_001852"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="U4B05_001852"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN2050984.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="U4B05_001853"
     CDS             9342..10199
                     /locus_tag="U4B05_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2050985.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="U4B05_001854"
     CDS             complement(10280..10615)
                     /locus_tag="U4B05_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050986.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="U4B05_001855"
     CDS             complement(10682..12052)
                     /locus_tag="U4B05_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN2050987.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="U4B05_001856"
     CDS             complement(12130..12666)
                     /locus_tag="U4B05_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2050988.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3769 a         2574 c         2118 g         4226 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttca
//
LOCUS       DASJST010000082        12670 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJST010000082 DASJST010000000
VERSION     DASJST010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.082"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U4B05_001857"
     CDS             6..305
                     /locus_tag="U4B05_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2050989.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U4B05_001858"
     CDS             378..1739
                     /locus_tag="U4B05_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN2050990.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U4B05_001859"
     CDS             1761..2021
                     /locus_tag="U4B05_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2050991.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U4B05_001860"
     CDS             2035..2787
                     /locus_tag="U4B05_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN2050992.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U4B05_001861"
     CDS             complement(2881..3486)
                     /locus_tag="U4B05_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2050993.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U4B05_001862"
     CDS             complement(3539..4513)
                     /locus_tag="U4B05_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN2050994.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U4B05_001863"
     CDS             complement(4891..5610)
                     /locus_tag="U4B05_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2050995.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4B05_001864"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4B05_001864"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2050996.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U4B05_001865"
     CDS             7161..8558
                     /locus_tag="U4B05_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2050997.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U4B05_001866"
     CDS             8639..9079
                     /locus_tag="U4B05_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2050998.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U4B05_001867"
     CDS             9227..9553
                     /locus_tag="U4B05_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2050999.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U4B05_001868"
     CDS             9554..10303
                     /locus_tag="U4B05_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051000.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U4B05_001869"
     CDS             complement(10383..10838)
                     /locus_tag="U4B05_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2051001.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="U4B05_001870"
     CDS             complement(11265..11540)
                     /locus_tag="U4B05_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051002.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="U4B05_001871"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="U4B05_001871"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN2051003.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4054 a         2175 c         2588 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DASJST010000083        12605 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJST010000083 DASJST010000000
VERSION     DASJST010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.083"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..524)
                     /locus_tag="U4B05_001872"
     CDS             complement(<1..524)
                     /locus_tag="U4B05_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2051004.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKT"
     gene            666..1598
                     /gene="pfkB"
                     /locus_tag="U4B05_001873"
     CDS             666..1598
                     /gene="pfkB"
                     /locus_tag="U4B05_001873"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN2051005.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1580..3043
                     /locus_tag="U4B05_001874"
     CDS             1580..3043
                     /locus_tag="U4B05_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2051006.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3040..3498
                     /locus_tag="U4B05_001875"
     CDS             3040..3498
                     /locus_tag="U4B05_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2051007.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3495..4469
                     /gene="lacD"
                     /locus_tag="U4B05_001876"
     CDS             3495..4469
                     /gene="lacD"
                     /locus_tag="U4B05_001876"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN2051008.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4910..5638
                     /locus_tag="U4B05_001877"
     CDS             4910..5638
                     /locus_tag="U4B05_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2051009.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5656..6852
                     /locus_tag="U4B05_001878"
     CDS             5656..6852
                     /locus_tag="U4B05_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2051010.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6845..7840
                     /gene="lacD"
                     /locus_tag="U4B05_001879"
     CDS             6845..7840
                     /gene="lacD"
                     /locus_tag="U4B05_001879"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN2051011.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7837..8778
                     /locus_tag="U4B05_001880"
     CDS             7837..8778
                     /locus_tag="U4B05_001880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEN2051012.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            8954..9433
                     /locus_tag="U4B05_001881"
                     /pseudo
     CDS             8954..9433
                     /locus_tag="U4B05_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9446..10336
                     /locus_tag="U4B05_001882"
     CDS             9446..10336
                     /locus_tag="U4B05_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2051013.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10323..11132
                     /locus_tag="U4B05_001883"
     CDS             10323..11132
                     /locus_tag="U4B05_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2051014.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11146..11547
                     /locus_tag="U4B05_001884"
     CDS             11146..11547
                     /locus_tag="U4B05_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2051015.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11593..12543)
                     /gene="yidC"
                     /locus_tag="U4B05_001885"
     CDS             complement(11593..12543)
                     /gene="yidC"
                     /locus_tag="U4B05_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2051016.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         4027 a         2046 c         2770 g         3762 t
ORIGIN      
        1 gttttctcac cgttcaaaga aacaaaaata acgctgtctt tcttagttag tttccgactg
       61 atgcttttga taatttctgg atcagtatgc atctgacaat tttttcccgt taattgaagt
      121 tttaccagca tttcttggcc aatcatttct gaaaatcctc gggcaaagac aaaaacccgg
      181 tcagcaaatt tgattctttg tattgcatct tcaatgttcc cgatttccaa catattgatt
      241 gtacgtacca cttcttgttc attttttaga atggctcggc ggattccttt gtctacttta
      301 tctaaaaatg aaaaattgat gttcttgttt tctccttctt ttagatgatg cttaaacgaa
      361 gtaaaccctt catagccttt ctttttcatt gtccgcacaa tggtggctgt tgagacattt
      421 gcttgttcac ttagtttcac gatggaatag ttagggatct ctaggatgag gtgttcgata
      481 aagttccaga ggtactgctc tgtttcactt aatttgtgat tcatattctt tccttctttc
      541 accaaaagtt agttaaaaaa agcacgacga gtttataaat gtaaaggttt tctttgtgtt
      601 gggacgatta agaaaacatt ttctatttcg cgtatttcat aatagaatca aggaggtcgt
      661 tggttatgtc tatctacaca tgcacgatga atttggcaat tgatctattt attgaaacag
      721 atgagatgca tccttttatg gtaaaccgca caaaagagga tgacattcag gcaaacggaa
      781 aaggggtgaa tgtgtcacta gtattaaaga tgctagattt cccaagtacg gcattaggtt
      841 tttctgctgg gttcacagga aagtatatag aagactattt acagcagaaa caaatccaaa
      901 cagagtttat tgaagtgcct ggtatgacgc gtatcaatgt atttacacaa gtcaatcaaa
      961 cgggagaaga atacaaatta gtgaatcaag gacctgaaat accgcagaca gctgttcata
     1021 attttttaaa tcaaataaga gcattacaag caggggatta tctttgtgtt tctgggagtt
     1081 taccaagagg gctgtctcca aaggttttaa tagaaatcag tcggatttgt ttgattattg
     1141 atagtagtga tcaagaagtc atggattgtt taccctatcg accgtttttg ctgaaaccaa
     1201 atgaggaaga actttcttct tggttcggac gaaaaatggt tacagatgaa gattactttg
     1261 tttatggaca acgactagta gagtcaggcg cagaaaatgt tttgttatcc ctcggtgaga
     1321 aaggagcgat tctttttacg aaagacaggg tgattcgagg aaactcgcca aaaggaaaag
     1381 ttgtcaatac agcatgtgca ggtgatgcca tgttaggcac ttttttagca ggatacatga
     1441 atcgacgatc gcttgatgaa acgttacgta aaagcatagc agcaggcagc tctactgctt
     1501 ttcgtaaagg actgactgat tttttggatg tggaggaatt aagtaaacaa atcaaaatca
     1561 aggaagagag gttcgaaaga tgggaaaata tcaactaatt gcagcaaccg gttgtccgac
     1621 aggaattgcg cacacctaca tggcgcaaga ggcattagag caagcagcta ggaaaaaagg
     1681 catcacgatc aaagtggaga cccatggaca aattgggatc gagaatgaat taacggctga
     1741 agaaatcaaa caggcagatg ctgtcattat tgccgcagac aaagatgtcc attcagaaag
     1801 atttgtggga aaacgagtca ttgatgtttc tgtcagtaca ggaatcaagg atgcacaacg
     1861 gctgattgac gaagcattgg caggaaaagg tactgtctta gcagataatg aagctgctga
     1921 tactttagaa ccagaagcga taagtggtgc taattttggt cgtagcatct ataaaaattt
     1981 gatgaacggt gtctctcata tgttgccttt cgtggtagct ggcggagtat tgattgctat
     2041 ttcatttgcc gtttggggaa tctattcttc tgatccagat catgcacaat acaatgctac
     2101 agcggcaatg gtaaaagcag ttgacgatgc agcaatggga atgatggttc cagttttagc
     2161 agcttatatt gcagaaggga tcgcgaaacg tccagggtta gtcgttggat ttgttggtgg
     2221 aatcgtagcc atgaatggcg gtactggatt tttaggaggt attttagctg ggttcttagc
     2281 aggttatttt atcttatttt tgcaaaagct actgataatt ttacctaaac aactagatgg
     2341 cttgaaggca atcgtcttat atccagttat tggtgtagca tttattggca tcgtgatgtc
     2401 tttgctggca ggtccaatga aggctataaa tgaaggaatg atgagctttt tagctggatt
     2461 tgaatattcg aatccacttg ttcttggcgt gattgttggc tgtatgtgtg cgtttgatat
     2521 gggaggaccg gttaataagg cggcatatgt aacaggaaca gctcttttga cacaaggaaa
     2581 cacgactttt atggctggtg cgtcagcagc ttgcattgca ccaccgttga ttactggttt
     2641 tgctacttta ttgtttggta aatattttga tactaatgat cgtaatgcag gtttggttaa
     2701 ttttatttta ggatcaactc atattacgga aggagctatt ccgtttgctg cgaaagaccc
     2761 gatccgagta ttaccaacga tgatgttagg ctcatcaatt gctgctgtct tgacctatct
     2821 gtttggtgtc caagtaccag ccccacatgg cggtttttta gtgttacctg tagtgacggg
     2881 aaaaatccaa tgggttacag cgattcttat tggttcgatc gttggtggag tgatactagg
     2941 tttgatccaa aagaaaagag taactgataa agacaagaag agggataata aagcaacaaa
     3001 tcgtacagtt atcgaagaag gaagagcagg aggtttatca tgacaatcat tcatgaagag
     3061 aacatttttt tacaacagcc attagataat caagaagaag catttaagtt tttagcaaag
     3121 caaacattct gtttaggaat cgctcatgac gaaaaagaag tatatcaaaa acttctggaa
     3181 agagaaaaag aaggtactac aggaataatg tcaggttttg ctatccctca tgcgaagtct
     3241 caaacaatca atgaacctag tttgattatc gtcacactga aaaaaggaat tgaatggaac
     3301 agtttagata gacagtcagt tgattttatt tttgcgttat ttatcccaga ttcagaagca
     3361 ggaacgactc atttgaaatt attgtctctc gttgcacgat cattgatgcg aaaagaggtt
     3421 acagaacagt tgagagctgc aaaatcaaaa atagaaatag ctacaatatt aactaaagaa
     3481 ataggtgaag atatatgaag aaaatgagta tagaaaaaca ggaacgtttg aagcgattga
     3541 cggatgaagc tggaattatt agcgcgctag ctatcgacca acgtggtgct ttgaagaaaa
     3601 tgatcaacca atataaagaa gctacatcag aagatatcat ccaatttaaa cagatggttt
     3661 ctcagtacct gactccgtat gcttccgcta ttttactcga tccagaatat ggtctgcctg
     3721 cagcagcgct tcgagagaaa gaatgtggtt tgttgctagc ttatgaaaaa accggctatg
     3781 atgcgaaaat gccaggccga ctaccagatt tactgaatag ttggtcagtg aaaaggttaa
     3841 aagaagtggg tgcggatgct tgtaaatttt tattgtacta tgacgttgat gaaccagtag
     3901 agattaatgt gcaaaaacaa atagttatag aacggattgg ttcagaatgc gaagcagaag
     3961 gcataccttt ctttttagag ctcctttctt acgatgcaac tattggcgat ccgtcttctc
     4021 ttgactatgc aaaagtaaaa ccgcataaag tgattgaagc catgcgagaa ttttcaaaag
     4081 aacgttatca agtggatgta ttgaaagtag aagtaccagt gaatatgaga tttgtggaag
     4141 gatatgggca agatacttgt tatagtcaag cagaggcatc cgcgtatttc tatgaacaat
     4201 cgaaagtgac tgaacaaccg tttatttttt taagtgcggg agtcagcgct gcactatttc
     4261 aagatacatt gcgttttgca aaacaatcag gttcgacatt caatggtgtc ttatgtgggc
     4321 gagcaacatg ggcgaaggga gtagcacctt ttgttaccga aggcgaagaa aaagcgagta
     4381 gttggatgtt agaagaagga aaacaaaata ttaaagaatt aagtacacta ttagaagaaa
     4441 cagcaacttc tatttatgtt aacaagtaag agaaaggatt gtctgagtga cagattcaaa
     4501 aaataattag ttatttaaaa aaatgtttgt caaattttat agaaaaaaag tataaaactg
     4561 caaaagttat ctaaaactaa taataacatt tgtcattaat aagctatttt tagttgttgt
     4621 aaatgatatt ggaagtaata aaacgatagt tgtaggaata tcatagggat taatgtggat
     4681 cacgctgctc gataagttag accattttga cggatcgctt atgtggttcg tctttttttc
     4741 tgttctttta ttggtaactg gtatttttga ggtatctttg tctattcaag atataaagct
     4801 ttaaagagct gttataatag cttttttggt ctaaaaaacc tttgacaaat gattaataat
     4861 tcactattct tgtgttgtgg ttataaccag taaagaagag gtgattttta tggagagctc
     4921 aaataaagga aaatcattgt attatcaact agttgattta ttgaaggatc gtatcgaaac
     4981 acagatgact ccgcatgata agctgttatc tgaaagagaa ctttgtcaac actatggggt
     5041 gagtcgtacc accgtacgtc tggctttaca ggaattggaa aatcaaggtt atatttaccg
     5101 aagacatgga aaaggtacct atgtctctga tttgtctgat aagtcatcga acttagctgg
     5161 agcatatagc tttacagaag aaatgcgcga aatagggcgc gtccctaaga cggtcatttt
     5221 agaatttaga caaatggaag ctaataagag aattgctaag aatttgaaca tccatttagg
     5281 tgaaactgta tttaaactta aaagattgcg tttggcagat gaagagccat tgatggttga
     5341 gcgttcttat ctaccggcga aactatttat gggacttgat ttgccgttac ttgaaagtaa
     5401 accgttgtat gatgtgtttt cacaggatta tgaacaacaa gttcaattag ctgatgaaga
     5461 gttttatgca agtatcgtgc aaaaaaaaga tgctgttgtg ctacgaatcg ctgatggcgc
     5521 tcccgtctta catctgacga ggataaccta taacgtgaaa aatgagatta tcgaatatac
     5581 agatagtgta gcaagagcgg accagtttca ttataaaatt cggcatatcc gaaaatgatt
     5641 gagagggaga acaacatgtt tgagctatcg aagaacaaat taaaacaaat aggtgcagca
     5701 atcacgacac aagaaataaa acaacaacca gatctctgga aagaaacaat cgatagattg
     5761 atgcaaagcc aagaaaattt agctctattt tttgatcaaa taaaaaaaga agcaaacgga
     5821 agaagaatac gtgttatttt tacgggtgca ggaacgtctc aatatattgg ggatacgctc
     5881 ttaccctatc taaatgaaca tggagacaat cagtttttta tcttccacag ttttggcacg
     5941 acagatattg tagcttcacc aaaagaattt ttgtttccaa atgaaccgac tttattagtt
     6001 tcatttgctc gcagtggcaa cagtccagaa agtgtagcgg ctgtgcaaat tgcaaatgaa
     6061 caagttgaaa cattgtttca tttgacgatt acttgtgcta aagaggggat gttggcaaaa
     6121 aacgcacagg atgataaaca gaattatctg ttattgatgc cagagagagc aaacgatgca
     6181 ggttttgcta tgacaggaag ttttacgtgt atgttattga gtgctttatg gatctttgat
     6241 caaacagcaa tggagaaaaa agaaagatat cttgaacaat tagttaaaat cggtcaagat
     6301 cttctttcaa gagatcaaga aattcaaaag ttattacctg atttttttga acggatcgct
     6361 tatctgggtt caggaagttt ggcaggtgcg acaagagaag cacaattaaa aattttagaa
     6421 ttaacagcag gtaaagttgc tacaattttt gattcttcga tggggtttcg tcatggacca
     6481 aagtcttttt taaatagcga gacactcgtt gtaggttttg tgagcaatca accatatacg
     6541 cgaaattatg acttagatat tttagaggaa ataaaagaag atcgggtcac tgccaatgtc
     6601 attgcgattg cgcaaccggg tactattgat ttctcaggta tgactgtgct attggaagac
     6661 tcggtgagta aattgcccga tgcctatctt gcattagcga tgattatgac tgcacaaatc
     6721 attgcactct atacttcaat caaagtagct aataaaccag atacaccatc tccaacaggc
     6781 acggtcaatc gagtagtcaa gggcgtcatt attcataagt atgaaaccga aggagaaatg
     6841 acagatgaat gaagaaaaaa atagggcaat ggagagacta tcaacaaaag atgggattat
     6901 tagtgcatta gcgatcgatc agcgtggtgc tctaaaaaaa atgatcaaat cgttgggaaa
     6961 agagccaagt gaagaccaaa tcagtgaatt caaagaacta gtatcagctg agctgacacc
     7021 ctatgcttct gctattttat tggatccgga atatggattg ccagcagcaa aagtcagagc
     7081 aaaagagacg gggttattgt tagcttatga gaaaaccggc tatgacgcaa ctacgcctgg
     7141 tcgtctacct gatttattag cggattggtc ttctctcaga ttgaaagaac agcaagcgga
     7201 tgcagtaaaa ttcttacttt actacgatgt tgatgaagca cccgaaatca atcatttgaa
     7261 gcatgtgttc atcgaacgtt taggtagtga gtgcgaagga gaaaatcgtc ccttctattt
     7321 ggagttagtt tcttatgacg cacaaataag cgatagttct tctccagagt atgcgaaagt
     7381 caagccccac aaagttattg agatgatgaa agaattttct gatccaaaat ataaagttga
     7441 tgtgttgaaa gtagaagtac cagtcaatat gaattatgtg aatggctatt ccacaggtga
     7501 atttattcac agcaaagaag aggctgctgc cttctttaag gagcaaagtg aagcgactcc
     7561 attaccattc atttttttga gtgggggtgt ttcaacagaa ttatttcaac aaacactgat
     7621 ttttgctcat gaatcgggtt ccaccttcaa tggtgtattg tgtggacgtg cgacatggaa
     7681 agatggggtc aaagcatacg tcgaaaatgg aaaagacgga gctattcatt ggttaagaac
     7741 aaatgggaga gaaaacatcg agactttaaa taaagtaata aatgaaaccg ctaccccttg
     7801 gttcaataaa acaaaaagca aaaaggtgaa gatgaaatga ttacgacagt taccttgaat
     7861 ccttccatcg atatcgcgta tacaattgaa gaattaaacc tcgacaaagt caatcgagtt
     7921 gaaacagtca ataaaacagc gggaggaaaa ggagtgaatg tggcgagggt tctaaattta
     7981 ttaggagaag aggtgatagc tactggttta ataggtggcc agcacggtga atatctaaag
     8041 aaccagctag atcagttagg gatcatgcat cagtttgttc caattaaagg agagacaagg
     8101 gattctattg ctatcctgca tagttcgaga caaacagagc ttttggaacg aggccccttc
     8161 ataacaaaga aagaacaaca gacatttacc gatcatttcg agcagctcct cgaaatatca
     8221 gatattatga cgatctcagg tagtttaccc caaggatgcc ctcttgatta ctataagcaa
     8281 ttggttgctt tagctgataa aaaagggaaa gacatattac tagatacttc tggtaaattt
     8341 ttgcttgaag tattggaagg taaggagaag ccactactta tcaagcccaa tctggaagaa
     8401 ttaggagaac tgattggcca acgtatctgt tcttcgattc aattagaaaa aattcagtcc
     8461 ttattagctc atccaatatt tgaaggagtg ccatgggttg ttgtttcttt aggagagaga
     8521 ggtgctgtgg caaaagttgc tcagtgtttt tacgaagtca ttattcctaa aatatcagtt
     8581 gttaatcctg ttggttcagg tgacgcgacc cttgcaggat cggcccatgc gttatcaaaa
     8641 aggcaatcaa tcgaagagat tctcgcattt tctatggctt gtggtatggc aaacgcgcaa
     8701 caagagaaaa ctggctggat cgaccctatg aaagttacga tgttgatgaa acacattcaa
     8761 gttaagaaaa tcaaatgaac ttaaatataa aagaaagcgt tttctttagt tgggaaggaa
     8821 tttgttctta gcttaaaaat gaaatataag aaagaaaaaa tgaaaacgtt atctttgaaa
     8881 cagaagggat atttgatgat gtcacagttt taattgaaca ataaatagaa aaaatagata
     8941 aggagaatgc atgatggcaa ttattggtgt aagaatcgat ggacgtttga tacatggata
     9001 agtagcaaat ctttggacaa cgaaactgaa tattagtcga atcatggtcg tggatgatga
     9061 ggtagccaca aacacaattg aaaaaagtgg attgaagtta gcgactccag caggggtaaa
     9121 attaagcatt ttgccaatcg aaaaagcagc aacaaatatt ttagctggca aatatgactc
     9181 tcaacgagta ttgatcgtag caaaaaagcc agatcgaatc ttatctttag tagaaaaagg
     9241 agttcctatc aaagaagtaa atgtggggaa tatgtcacaa acgaatgaaa cacggtccat
     9301 tacaaaatca atcaatgtgg tagatgcaga tatcgaagta tttaaaaaat tagatcagct
     9361 tggtatacat ttaattgctc aaatggtacc aaacgataaa gcagaagact ttatgaatct
     9421 tttaatcaaa taggaggaat gatccatgga tattcaatgg tggcagatct tattattaac
     9481 attatatgca ggttatcaaa ttttagatga attacagatc tattcttcaa tgagtgcgcc
     9541 agtgtttgca gggttatatg caggtttagt aatgggtgat ttaaacgcag gtttgattat
     9601 tggtggcagt atgcagttga cagttttagg agtggggacg tttggaggcg catcaaaaat
     9661 agatgccaac tctgggacga ttttagcaac tgccttctcg atttctcttg gaatgaatcc
     9721 agaacaagcg attgctgcta ttgcagtgcc tgtagcaagc ttaatgatcc aattggatat
     9781 tttagctaga ttcgctaaca catactttgc ccatcgtatt gaccgtcacg tagaagacat
     9841 gaattatcaa gcaatcgaac gcaatttctt aatgggagct ttaccttggt cgctctctcg
     9901 tatgattcct gtatttttag ctttagcatt tggtggtgga ttagttgaaa atgttgtttc
     9961 tgttttaaac gggaatctaa aatggctagg cgatggactt tcagtagcag gagctgtttt
    10021 accagctgtt gggtttgcca ttcttctgcg ctatttacca gtaaaaaaac atctatctta
    10081 tcttatttta ggatttacga tcacggcatt attagcaacg ttattctcta atgttcaatt
    10141 attaggaaca agtgtggcaa gcgttgttaa agatcttccg gcaactttca attcgctgcc
    10201 gatgcttgct gtagcattga ttggtctagc actagcaatg atcagttata agaatgatca
    10261 aaacaaagac catcaaccat tagctagtga gccatcacat tctgttgagg gggaaatcga
    10321 agatgacgaa atctaattac aaattaacaa aagaagattt taagcaaatc aatcgaagaa
    10381 gtttgttcac ttttcaatta ggttggaact atgagagaat gcagggttct ggctatttat
    10441 atactatctt gccacaacta agaaaaatat atggagacaa tacaccagaa ttaaaagaga
    10501 tgatgaaaac acatacgcaa ttttttaata cttctaattt ttttaataca atcattactg
    10561 ggattgatct tgcaatggaa gaaaaagagg gaattgcttc aaaggaaacc gtatcggggc
    10621 tgaaagctgg attgatgggt tcctttgcag cgattggaga ttccgtattt gctgctttga
    10681 ttccaacgat ttttggggct ttggcggcga atatggcaac tgatggaaat ccgataggtg
    10741 tgtttatatg gatttttgct cagatcgttg tcatggtttt tcgttggaag caacttgaat
    10801 ttgcatacaa agaaggaatt tccttagtta ctactatgca atctcgctta actgcattaa
    10861 cggacgcagc cactttatta ggggtattca tggtaggggc attagttgcg acaatgatca
    10921 atgttaaatt ttcatgggca ccaacgattg gcgatgtgac attgaatatg caaaactata
    10981 tcgatatgat cttacctaaa attttacctg ctgggatcgt cggtggtgtt tattggttat
    11041 taggaaagaa aaacatgact cctactcgag cgatttttat cgtattaatt gtgtgtgttg
    11101 ctttatctgc attgggtgtg atttcgaaat aagggagagc gaaaaatgag tagacaattg
    11161 gtattgatta gtcacggaaa attttgtgag gaattgaaaa aaagtacaga aatgatcatg
    11221 gggcctcaag aggatatcta cacgttacct ttattaccag aagatggcac tgaaacatac
    11281 aaacaaaagt ttctcactgt cacagattcg ttaaatgatt ttgtggtact atgcgatctt
    11341 ttaggtggta cacctgcaaa tatcatttct aaactaataa tggaaggaca acagattact
    11401 ctttatgctg ggatgaatct tccgatggtc attgaattta tcaactcaca aatgattggt
    11461 tcagagcccg agtttgttcg tgcaggatgt gaaagcattg tctctgtcaa cgaattaatt
    11521 aatggaatag cagaagagga agagtaacaa aaaagatgag ggcttagctc tcatcttttt
    11581 tgtcctatgt ttttatttcc gcttctgctg ctttcctgcg ttgcggcctt tgttgtttgt
    11641 actgtttttt ggttgtttga tttgttttgt tgcttgtttg ttgttagctg gctttgctga
    11701 ttctgtcacg tcttttcttg gagtgacgac ttgttttggc gggttttgtt tcaactcttc
    11761 tgccacttgt gctttcaaac gtggtttcat cataacgttt gtgatgaatg tttgcagaca
    11821 gctgaacaca ccaccgacta cccagtaaag tgtaacaccg gctggcgcac tgatcgacat
    11881 gaagacgatc atagcaggag agacgattag catcgtacgc atcgtctttt tctgttcttc
    11941 aggaataccg atcgttgaga tgtatccttg aagcagatag gcgacccctg caatgacaac
    12001 aagtaccata cttggtgagc ctaaatcgat gccgtagaaa gatgattccc gaataccttc
    12061 tgtataacgt gccgtaaagt acaaggcaga gaagatcggc atttggatca atagaggcag
    12121 acagccgatt ccgccggtca tactcaatcc gttttcgcgg tatacttgct gcatttctgc
    12181 ttgtgctgcc atttgttctt cacgtgttgt tgcagctttc aatttttgtt gggctgcttc
    12241 aacttgtggt tttaacgctt gcattttttc agattggatc attgtttttt tcgattgact
    12301 aatacctagt ggcaagatga tgatacggac gatcacagtc attgcaataa ctgcccagcc
    12361 ataagaccaa ttgaaattct caactagata tgtaatcgct tggcccatag gatggacgag
    12421 tactcgataa acgattcccg aagtgtcagg gtttccgttt gaatccatcc gcacacagcc
    12481 agataaaaag agcaggatac tgacaagtcc ggatcccaat aaccagtttt tccatttatt
    12541 catagaatac ttcctcttct ataattttta cacaaactta actatactaa aaaagcgcat
    12601 ggaat
//
LOCUS       DASJST010000084        12576 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJST010000084 DASJST010000000
VERSION     DASJST010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.084"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="U4B05_001886"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="U4B05_001886"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN2051017.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="U4B05_001887"
     CDS             1286..2461
                     /locus_tag="U4B05_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2051018.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="U4B05_001888"
     CDS             2465..3106
                     /locus_tag="U4B05_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2051019.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="U4B05_001889"
     CDS             3103..4020
                     /locus_tag="U4B05_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2051020.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="U4B05_001890"
     CDS             4017..4688
                     /locus_tag="U4B05_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2051021.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="U4B05_001891"
     CDS             complement(5229..5717)
                     /locus_tag="U4B05_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN2051022.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="U4B05_001892"
     CDS             complement(5918..6184)
                     /locus_tag="U4B05_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2051023.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="U4B05_001893"
     CDS             complement(6184..6447)
                     /locus_tag="U4B05_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2051024.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6923..7108
                     /locus_tag="U4B05_001894"
     CDS             6923..7108
                     /locus_tag="U4B05_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2051025.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7250..9073)
                     /locus_tag="U4B05_001895"
     CDS             complement(7250..9073)
                     /locus_tag="U4B05_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN2051026.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="U4B05_001896"
     CDS             complement(9671..12376)
                     /locus_tag="U4B05_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN2051027.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3615 a         2615 c         2378 g         3968 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgc
//
LOCUS       DASJST010000085        12432 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJST010000085 DASJST010000000
VERSION     DASJST010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.085"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..190)
                     /locus_tag="U4B05_001897"
     CDS             complement(<1..190)
                     /locus_tag="U4B05_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051028.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="U4B05_001898"
     CDS             complement(200..1015)
                     /locus_tag="U4B05_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2051029.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="U4B05_001899"
     CDS             complement(1277..1720)
                     /locus_tag="U4B05_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN2051030.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="U4B05_001900"
     CDS             complement(1854..2192)
                     /locus_tag="U4B05_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2051031.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="U4B05_001901"
     CDS             complement(2180..2557)
                     /locus_tag="U4B05_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2051032.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="U4B05_001902"
     CDS             2781..3974
                     /locus_tag="U4B05_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2051033.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="U4B05_001903"
     CDS             complement(4140..4562)
                     /locus_tag="U4B05_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051034.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="U4B05_001904"
     CDS             4957..5151
                     /locus_tag="U4B05_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051035.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="U4B05_001905"
     CDS             5148..5594
                     /locus_tag="U4B05_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051036.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="U4B05_001906"
     CDS             complement(5804..6976)
                     /locus_tag="U4B05_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2051037.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="U4B05_001907"
     CDS             complement(7182..7997)
                     /locus_tag="U4B05_001907"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2051038.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="U4B05_001908"
     CDS             complement(8147..8593)
                     /locus_tag="U4B05_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN2051039.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="U4B05_001909"
     CDS             complement(8590..9594)
                     /locus_tag="U4B05_001909"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2051040.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="U4B05_001910"
     CDS             complement(9634..9963)
                     /locus_tag="U4B05_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN2051041.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="U4B05_001911"
     CDS             complement(10089..12416)
                     /locus_tag="U4B05_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2051042.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4217 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aa
//
LOCUS       DASJST010000086        12387 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJST010000086 DASJST010000000
VERSION     DASJST010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.086"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            405..962
                     /locus_tag="U4B05_001912"
                     /pseudo
     CDS             405..962
                     /locus_tag="U4B05_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1020..3059
                     /locus_tag="U4B05_001913"
     CDS             1020..3059
                     /locus_tag="U4B05_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN2051043.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3168..4319)
                     /locus_tag="U4B05_001914"
     CDS             complement(3168..4319)
                     /locus_tag="U4B05_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN2051044.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4473..5003)
                     /locus_tag="U4B05_001915"
     CDS             complement(4473..5003)
                     /locus_tag="U4B05_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2051045.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5102..5452)
                     /locus_tag="U4B05_001916"
     CDS             complement(5102..5452)
                     /locus_tag="U4B05_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN2051046.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5609..7138)
                     /gene="ahpF"
                     /locus_tag="U4B05_001917"
     CDS             complement(5609..7138)
                     /gene="ahpF"
                     /locus_tag="U4B05_001917"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN2051047.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7148..7711)
                     /gene="ahpC"
                     /locus_tag="U4B05_001918"
     CDS             complement(7148..7711)
                     /gene="ahpC"
                     /locus_tag="U4B05_001918"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN2051048.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASAPIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7855..8517)
                     /locus_tag="U4B05_001919"
     CDS             complement(7855..8517)
                     /locus_tag="U4B05_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2051049.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8701..10347
                     /locus_tag="U4B05_001920"
     CDS             8701..10347
                     /locus_tag="U4B05_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2051050.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10338..11420
                     /gene="uxuA"
                     /locus_tag="U4B05_001921"
     CDS             10338..11420
                     /gene="uxuA"
                     /locus_tag="U4B05_001921"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2051051.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11528..>12387)
                     /locus_tag="U4B05_001922"
     CDS             complement(11528..>12387)
                     /locus_tag="U4B05_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEN2051052.1"
                     /translation="SVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
BASE COUNT         4130 a         2291 c         2328 g         3638 t
ORIGIN      
        1 ttaaagattg ttaaatttca ctatttcatt tcaaattcca ttgcctaatc attaggaaaa
       61 gtctatcatg aactcgtaaa tagaatatag taataacctt gttaggtgag gctcctatac
      121 aaacataggc tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca gaaactgttg
      181 gattaaggct ttttccaata gctaagaatg ttttctttac gttgtatagt gctaaaactc
      241 ggacgatgag atgatgctag gtcagaattc ttgttcgaaa aggtttctga ccttttcttt
      301 atatttttgt gactgactta tttgatgaga caaactcgct cctcacctga caagtggatt
      361 gctgtgtgag gaggagatgc ggtgaaaaaa gatcgattgg tggaggtcgc tcggattcac
      421 ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct atcatgagag
      481 attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct tactcttatg
      541 ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa gctccctatt
      601 atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt cctggtggag
      661 tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac aaaggagagc
      721 aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac aaagaaggag
      781 acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag cagctgaaag
      841 acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt agtatccaaa
      901 cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa gacatatgtt
      961 agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa
     1021 tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga
     1081 tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa gaaacccaag
     1141 cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac aagggagatt
     1201 ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa
     1261 aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa ggacttcaga
     1321 tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca tataaagcaa
     1381 agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca ttagacagaa
     1441 atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa atggaaggaa
     1501 atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata gatcgaaatg
     1561 gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca agtaaacttg
     1621 gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc gacttaacag
     1681 aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac tttgtcccat
     1741 tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt caagaagtag
     1801 acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac gtgggagata
     1861 tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa atcggtcgct
     1921 caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg aaattaagca
     1981 ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt caaaatggca
     2041 aagatatcaa attgacaatc gatgcgaagg cacaaaaaat agcttttgac agtctaggag
     2101 gaaaagctgc atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta
     2161 gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa gattacaaag
     2221 cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt tatgctcctg
     2281 gatctacgtt taaaatgatt acagcagcaa tcggtctcga caacggcact atcgatccaa
     2341 atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg ggatctttta
     2401 aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct ttgatctatt
     2461 ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag
     2521 gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg aatccagcac
     2581 aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact ggatatggac
     2641 agggcgaact tctaattact cctatccagc aagcagcaat gtattctgtt tttaccaaca
     2701 atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat aagaagaatg
     2761 tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt gtgcaagatg
     2821 taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg aaaactggta
     2881 cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc ttgtttgctt
     2941 tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg
     3001 attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat tatcaataaa
     3061 aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc cttttttcaa
     3121 ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca cacttcataa
     3181 actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa taaaatcacg
     3241 ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa
     3301 atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa tgaactatgt
     3361 tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt caaaatcgct
     3421 acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa atcaacttct
     3481 aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc ctctacattg
     3541 tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa aaaagcacca
     3601 atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca aataactagc
     3661 gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag taggaaaggc
     3721 gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca
     3781 agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct gaaacttaga
     3841 actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga tccttctttt
     3901 gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga agttgccgca
     3961 agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac
     4021 cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc aggtcggaaa
     4081 tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa taagatcaca
     4141 ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa tccagcttgg
     4201 cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa tctccgcgag
     4261 atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat aaattccatt
     4321 ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat tattgaaaaa
     4381 ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg attgttggtt
     4441 tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt tcttattctg
     4501 atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa tatttggaat
     4561 agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat caacaacaga
     4621 atactactat aaacactcca acttttagct tttgtattca tgctgaagaa caactggaac
     4681 aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac gattgcaggt
     4741 gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa cagaccgata
     4801 ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa caacccaagc
     4861 aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga agggtttagc
     4921 acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta tttttgaata
     4981 ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt aacgtgaact
     5041 agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa
     5101 atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg atcattttat
     5161 ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt acaccacaaa
     5221 caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca aaaaattgtt
     5281 cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa cccgtcagcc
     5341 aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct tttgctacat
     5401 acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc ataatcagtc
     5461 acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga gaagaaaaag
     5521 cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc cttttttccg
     5581 ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg aatgctccta
     5641 aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga ctatctgtac
     5701 aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg gtcactattt
     5761 catttcttgg attcatttcg atcgttctat ctagccaatc cgtattaggg agtaagccga
     5821 ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg cgatcgatat
     5881 aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa
     5941 cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct tttaattctg
     6001 gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt gctgcttcaa
     6061 caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa gggccgtcac
     6121 agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt acattgatag
     6181 aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat ataccgttag
     6241 ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt tgattcatca
     6301 taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat tttggacctt
     6361 ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact tgtccgccga
     6421 aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata gctgcactac
     6481 tagctgctgg tccaccgcca atcactaata catcataagg ttctttttca aacaactctt
     6541 cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg cgtccgctac
     6601 taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta tcatcgattt
     6661 ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt aatacagcta
     6721 atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta acataactgt
     6781 caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca ctgattttcg
     6841 gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg
     6901 ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa aaactaggtg
     6961 tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct atttccttcg
     7021 taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa aaaacaatag
     7081 gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg tctaacatat
     7141 ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg ccactttctt
     7201 tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca gcgcgaatct
     7261 tatcaatcgg tgcactagcg tcacgaccga ttccatctgc attgatttcc atagcttgaa
     7321 cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc tcttcatcca
     7381 aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg tattcgattt
     7441 taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta tcggtagagc
     7501 aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt aagtcttcta
     7561 attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata ctccagttcc
     7621 ccataagatc tttttcactg acttctaaaa attctccttg atgataagca tgtgcattaa
     7681 attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata attatttatt
     7741 tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt atttaataaa
     7801 gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg
     7861 ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa
     7921 gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa
     7981 atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca
     8041 gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc
     8101 tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc
     8161 atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg
     8221 tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa
     8281 gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac tttgaggtat
     8341 cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc
     8401 gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc
     8461 atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc ttttcatctt
     8521 tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct
     8581 ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat
     8641 ttttattgca taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa
     8701 atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa
     8761 atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc
     8821 catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg
     8881 gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt
     8941 gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga
     9001 gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt
     9061 tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact
     9121 tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg
     9181 attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct
     9241 gcgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat
     9301 aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg
     9361 caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt
     9421 gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa
     9481 atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat
     9541 attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc
     9601 aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc aacggtagat
     9661 aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt
     9721 atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg
     9781 ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc
     9841 attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt
     9901 cctgatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt
     9961 gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata
    10021 ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca
    10081 ttcatcccga gtccagaccc actattgaca gactttcagc ataagctttc cgatattcaa
    10141 ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa
    10201 gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc
    10261 atcaagatgg tagcaggacc gaatgccgta cgaaaaacat tacaggatta cttacaagaa
    10321 catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga
    10381 ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg
    10441 atataccagt aggagaggta tggcccaggg agaggatcaa agaactaaaa gaagaaattg
    10501 agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa
    10561 ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg
    10621 cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat
    10681 ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata
    10741 ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttgaat
    10801 tgccgggatg ggaacctgag cgcttagctc atatcaaatc cttgcttgaa gcctatgcat
    10861 cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa
    10921 catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt
    10981 ttggattacc tcgaatcgta aagaatcgag aagatttaga ttggttgtgc cgcatagtag
    11041 acagccctta taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg
    11101 atgtatatgg gattttagca gaattttgtc aaagagatcg aatcccattt gcacatgttc
    11161 gaaatattaa atttttagct aatcgagatt tctatgaggc accgcataaa tccgaatacg
    11221 gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt aacgaataca
    11281 tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat
    11341 atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa
    11401 acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg
    11461 ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt tatgtatctt
    11521 atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt gcttcttctt
    11581 ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct tctggtgtga
    11641 tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc aaagggcagt
    11701 cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc gattcatttg
    11761 aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca cgcgtatagt
    11821 tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt actaggtctt
    11881 caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt tgattttgtt
    11941 ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaac acgctatgtt
    12001 cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta agaaatccat
    12061 tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt tcaggtataa
    12121 acaaatcaaa tacagtacaa aaggactcca ccatttctct tgaataaaga acatgaggaa
    12181 cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat tcgattttcg
    12241 tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa attggcagat
    12301 taggtcgcaa aatatctaca gagggatcaa taaaaaagtc aatatattca tactctgtga
    12361 attctttttg tacgtctttc acagaag
//
LOCUS       DASJST010000087        12295 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJST010000087 DASJST010000000
VERSION     DASJST010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.087"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            16..2034
                     /locus_tag="U4B05_001923"
     CDS             16..2034
                     /locus_tag="U4B05_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2051053.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2104..2310
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2461..4875
                     /locus_tag="U4B05_001924"
     CDS             2461..4875
                     /locus_tag="U4B05_001924"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN2051054.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5223..6776
                     /locus_tag="U4B05_001925"
     CDS             5223..6776
                     /locus_tag="U4B05_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN2051055.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7157..8941)
                     /locus_tag="U4B05_001926"
     CDS             complement(7157..8941)
                     /locus_tag="U4B05_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051056.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8956..10677)
                     /locus_tag="U4B05_001927"
     CDS             complement(8956..10677)
                     /locus_tag="U4B05_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051057.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10777..11538
                     /locus_tag="U4B05_001928"
     CDS             10777..11538
                     /locus_tag="U4B05_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2051058.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4028 a         2197 c         2472 g         3598 t
ORIGIN      
        1 gttggaggat agaagatgga aaacattgca agaaaagaac ggagacgttt agaacagacc
       61 aaacgttttc gaaaagcaaa acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt
      121 tctgtcgctg ccccattagt acagccggtt cctgttcaag cggatgaaac acctgttcag
      181 ttcggcgcaa gaatcaatac agcggcattt atcacagaaa ttgctactta tgctcaacca
      241 atcgctcaag ccaacgatct atatgcatca gtgatgatcg cacaagcagt tgtagaaagc
      301 ggttggggca gtagtgcgtt atctcaagca ccttattata atctttttgg gataaaaggc
      361 agctatcaag gacaaaccgt ctacatggat acgcttgaat ttttaaataa tcaatgggta
      421 aataaaaagg agcctttccg acaatatcct tcttttgctg aatctttcaa tgacaatgcc
      481 tatgtactta gaaatacttc atttggcaac ggttattact atgccggtac ttggaagagc
      541 aacacgaaat cctatacaga tgcgacagct tgtttgacag ggcgttatgc gactgatcct
      601 ggttacgcag gcaaattaaa taatatcatc gcaacttacg gattgaccca atatgataca
      661 ccagctagcg gaaacgcagg cggtggagcg actacaggtg gcaataatac tggaagtgca
      721 ggtagtactg gcggttcaag caattctggt aatacaaact caggttcagc aacttcgacc
      781 acttatactg tccaatctgg tgattctgtt tggggaatct caaataaatt cggtatcaca
      841 atggatcaat tgatccaatg gaacaacatc caaaacaatt tcatttatcc aggacaaaaa
      901 ctgacaatca aaggtagcca ggaaaatggc tcttcgacga ataattccgg taacaatacc
      961 aactcttcag gaaatgctgg tacatcaaac ggaggacaaa caactggtgc gaaatacact
     1021 gttcaatctg gtgattctgt ttggaagatc tcaaatgatc acggaattac gatggctcaa
     1081 ttgattgaat ggaacaatat caagaacaat ttcgtttatc caggacaaca gctaatcgta
     1141 tcaaatggcg gttcttctaa tgctggagca aatactggct caaatactgg gaatacaagt
     1201 ggctctagtt cgtccagctc aagttcttct aatacagctt caggaacgaa atatacagtt
     1261 aaagccggag actctgtttg gagcgtctca aataaatacg gaattacgat ggctcagcta
     1321 atccaatgga acaatatcca aaataacttt atctacccag gacaacaact gactgtttca
     1381 aacagtggat caacaaacag cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac
     1441 agcagttcag ttaatactgg cagcagttcg tctcaaggca cttataccgt gaaagcaggt
     1501 gaatctgttt ggagcgtggc aaataaaaac ggcatcacga tggatcagtt aatcgaatgg
     1561 aataatatca aaaacaactt catttatcca ggacagcaat tgatcgttaa aggtggaact
     1621 tcggcaaata caaatacagg ttcaactact tctgcagcga aaccaaatac accgaatacg
     1681 tctgctacga caagtacatc atccactggt aatacgatgt ataccgtgaa agcgggcgag
     1741 tcagtttgga gcgtagcgaa caaacatcat atcacgatgg atcagttaat cgaatggaat
     1801 aatatcaaaa ataatttcat ctatccagga cagcaagtga ttgtgaagaa aggttcatct
     1861 caatcaacaa accaacaatc agttactggt tcgaaaacct atactgtgaa atctggagaa
     1921 tctgtttgga gtgttgctga cagtcatgga attacaatgg ctcaactaat cgaatggaac
     1981 aatattaaga acaactttat ttatccaggt caaacgttga ttgtagctaa ataaaatagc
     2041 tgttcgaaga aataacgatt gaattccaca aaaatatttg ttactataag gctacttaaa
     2101 tatgacaaca cttgaaaagg aagtagtaaa aagactgaag tttcagagag cgcatggctg
     2161 gtgagaatgt gcacggatgc ttttgaactc gcctttgagt tgcccttgct gaatacccga
     2221 gtaagcaagc ggcggtgacg gccgttacaa cgttcgaggc cttttcacta aggtaaaatt
     2281 aggtggtacc gcgtagatta cgtcctataa aaagagcaat ctttttatag gattttttta
     2341 tttcttttgt gaagtttgag aaaacaaagc aaaaggaaat aaaaataaac agattctgcc
     2401 ggttttttcg ttatatttat aatagaaaca agtgttttca atagaacagg aggacagcaa
     2461 atgagttaca atcataaaga aattgagaaa aaatggcaaa aatactgggc aaaaacgaat
     2521 actttcaaca cccatgatga tcctgaaaag ccaaaatttt acgcactgga tatgtttccc
     2581 tatccatccg gtcaagggct acatgtaggg catcctgaag gttatacggc aacggatatc
     2641 ctttcacgtg tgaaaagaag tcaaggatac aacgtgctgc atccaatggg gtgggatgca
     2701 ttcggtctgc ctgctgaaca atatgcgcta gatacaggaa acgatccagc tgagtttacc
     2761 caaaaaaata tcgaaacatt ccgacgccaa atcaattctt taggatttag ttatgactgg
     2821 aatcgcgaag tgaatacaac tgatccggag tattataaat ggacacaatg gatctttacg
     2881 aagctttatg aaaaaggtct agcttatgaa gcagaagtag cggtaaactg ggtacctgaa
     2941 ttaggaacag ttatttcaaa tgaagaagtg atcgatggga aaagtgaacg tggtggctat
     3001 gatgtagtca gaaaaccaat gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc
     3061 ttattggatg atctagaatt agttgattgg ccagaaagca ttaaagaaat gcaaagaaat
     3121 tggatcggtc gttcagtagg agcaaatatt gaatttaaag tggctggtac ggataaatcc
     3181 tataccgtat ttacgacacg accagatact ctattcggtg caacttactc ggttcttgca
     3241 cctgaactag atcttgttag agaaattacg acccctgaac aaaaagctgc tgtagaagct
     3301 tatatcgaag agacagctaa aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa
     3361 acaggagtat ttactggtgc ttatgcaatc aatccagtta atggcaagga aatcccaatc
     3421 tggatcgccg attatgtatt atcttcatac ggcacaggag ccatcatggc tgttccagca
     3481 catgatgaac gtgactttga attcgctcaa actttcggac tagaaattct tccagttatc
     3541 gaaggtggag atgttgaaaa agctgcttac acagaagatg gtacgcacat caactctgaa
     3601 tttctagacg gcatgaacaa acaagaagca atcgataaaa tgaatgaatg gctagaagaa
     3661 aatggcgtag ggaaaaaaga agtcagctat cgcttacgtg actggctatt ctcccgtcaa
     3721 cgttactggg gtgaaccaat cccaatcatc cattgggagg acggaacagt cacagctgtc
     3781 ccagaagaag agctaccatt acggttgcct aagacaaaaa acatcaagcc aagcggtact
     3841 ggagaatctc cattagcaaa tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt
     3901 aaaaaaggcc gtcgcgaaac gaatacaatg ccacaatggg caggaagctc atggtattac
     3961 ctgcgttata tcgaccctca taacaaaaaa gaactagcag attatgaaaa gttgaagcgt
     4021 tggctaccag ttgacatcta cattggtgga gcagaacatg cggtacttca tttactatat
     4081 gcacgattct ggcataaatt cttatacgat atcggtgttg ttcctacaaa agaaccattc
     4141 caaaaactat acaatcaagg aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct
     4201 cgtggtaatg tagtcaatcc tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc
     4261 atgtacgaaa tgttcatggg gccattagat gcttcgattg cttggagcga aaatggctta
     4321 gaaggaagcc gtaaattctt ggatcgtgta tggcgtttga tcgttgatga gaacaataaa
     4381 atgcgtgacc gtatcactac attgaatgat ggcaagcttg ataaagtgta tcatcaaaca
     4441 gtgaaaaaag tgacagaaga ctatgaaaat cttcatttca atacagcaat ctcccaattg
     4501 atggtcttta tcaatgaagc ctataaagtg gacgctctgc cttatgaata tatcgaagga
     4561 tttgttcaac tactcgcacc gattgctcct cacatgggtg aagaactatg ggcaatcctt
     4621 ggaaatgatg gtggtatttc ttacgcacca tggccaacat acgatgaagc agcacttgtt
     4681 gaagacgaag tagaagttgt tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt
     4741 ccccgagact taggaaaaga tgagctagaa aaagcagcat tagccaatga aatagtgcaa
     4801 gaatatattg aaggcaaaac agtcagaaaa gttattgcgg taccaaacaa gttagtcaat
     4861 atcgttgcta attaattggc tctttgtcaa taaggactga tgagttgtgc aaaatcaaat
     4921 ctgggccaga atgaacttca ttctgtctca gattttttgt tatttcaaat tgattaattg
     4981 attgatcaaa aaggttttga ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg
     5041 aatggtcttg taagagatct ctgtaatcat caaatttttg tctattaatc ttagtatttt
     5101 ttgatgtata aactacctga aattgagagt acataccaaa ttcataaatt ttttattagt
     5161 gggactagtg attgtatgga ttttgacgca taatgaatga agtatgagaa aaggagagac
     5221 aaatggatca agaactattt attatcgaag tattatcagc taaacaaaaa attatcgaga
     5281 aagcgaaaaa aagagcgaaa agaataatgt tcaagctttt aagcttagta tttttctctg
     5341 gtttgctttt tttcggagct gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt
     5401 cagctgaagg gatgcaaaaa ttaaataatt tgtatcactt ttcgtgggga gttggcggaa
     5461 taacaattgt tgtgcttatt gtttttcttc tacttatttg gctccgttac catcaattgt
     5521 cgcgtcattt cgatcaatgg cttagtcaaa gagcacaaca gtttttacat aataaaccaa
     5581 cttctcaaga tgatgactat ctttattttg agaagacttc gacgagtcaa gcttttcgga
     5641 tacaaaaaag tgattgccag ttattgctga gatcacttga tgatttacct atctacttag
     5701 gcgaaagcaa atcaaaattc ggaatgaaca cattccatat ttttttacta gaaaacgaac
     5761 ccagatggga aaaaagccag tcttcgcctg gtggatacct gacaactaag atgagtacat
     5821 tagtcactgg ttttttcctc attcttttag ctggaacaat ctattatggg agatacactc
     5881 agccaaaaat gtcttctcat ataaatgctg cttctcacat gacaatgaac tcaactaacg
     5941 atacgcatat acaaaagaca aatgaagggt tgttagctga tcctaagcaa aaggtaaatt
     6001 tgacaacaca aaagataaat gatctcaaac ttgatacaga gacgcactct ctctatatga
     6061 caacagatag tggtgctaat tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg
     6121 gcgaatacac cctgacaacg gggctgattc ctgaaggtta ttggatggac aacacatatg
     6181 atatttccgc cgatttctca tggtttatct attcttctgg ggatgatgta cgtctgctga
     6241 cttcaagaga taatggtaaa acatggcaaa cgtctattat cacccaatca gttcagccga
     6301 tacgttatcg aaaagcggta ttttatggca acgatggtct atttatctgt acatcagcca
     6361 gtgggatgtc tgcagaaact ttgtacattt acactactat ggatggcgga atcacttgga
     6421 taaaaagtgg aagtacaatg atcaatcagc cagtacaaaa tgtcagcttc gttacaactt
     6481 ctttaggttt tgtttcgaca cgtgaaagtt tgttttatac aaacaacgga ggacgctcat
     6541 tcaaagaatc agttctgtcg attccggaaa actatcagac aggggggata gatctttttc
     6601 aatcaccaaa tgaagttact caaataagcg cgaatacgtt agagacaaaa ttttatttgt
     6661 tgaaaaaagg accaatcgat gcagggaaaa tgtttgcctg cctcttccgt tcaactgata
     6721 acggggaaac atggcaattc gttcagcaac tatcgcaagt agaacaagga gattaaagaa
     6781 gagagattgt gagcctgacg ttcagcaccg agtcacaaga aacaaattca aaaaacagct
     6841 tctcatattt gttgaatagt gtaactcgtg attcggagtt gacaagagca acaagaagcg
     6901 attttgaagt atagcttctc gtattttaca agatcgtgtc ttattgccga taggttgtct
     6961 cttgaacacc gtttattcgt aaaaagggat tgtgacaagt cttttgtcac tacttaccga
     7021 acgaaactga aggagctatc caaaaagtat cattagtatt attctttgtg tttctagtgg
     7081 caccactagt acatttaaaa aagctatcga tcatggacta atcatacatg atcgatagct
     7141 ttttagataa tgtttgttac gctatctgaa attgactatt ataaagactg gcataaagcg
     7201 ttggtgattg gagtagttca gaatgtgttc ctttttcgac gatatcgcca ttcttcatta
     7261 caaggatcat gtctgcattt tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc
     7321 tatcttttgt cacagcttcc attgcttgct gaatcaatgc ttctgttcgt gtatctacgc
     7381 tagaagttgc ttcatctaaa ataacgacag gaggattcgc tagaatgact cgagcaattg
     7441 tcaataattg ttgctgtcct tgagataacg tgccgttttc gctagaaatg atcgtatcat
     7501 atccgtctgg cagtgttcga ataaagtgat cacattgtgc gatttcagcc gcttcgataa
     7561 tttgttctct tgtggcatcc attttcccgt aggcgatatt atcagctacc gttccttcaa
     7621 acagccaggt attttgtaaa accatcccaa aagaacgacg tagttctcca cgtgaaagct
     7681 tggtaatatc aagaccgtcg aattcgatct ttcctttatt cagttcataa aaacgcatca
     7741 gcagattgac taaagtcgtt tttcctgcac cagttggacc aacgatcgct accgtttgtt
     7801 ttggctttac tgaaaaatcg acttgattca tcaatatttt atctgatgtg taaccaaact
     7861 gtacattttt gaaagctatt tttcctttcg gttgttctaa aagtcttgca tctggaaggt
     7921 ctggaatttc atctgtttca tctaatatct caaatatcct atcgattgct gccattgcgg
     7981 attggatcga gttgatcaca taagaagcgg tagaaattgg ttcagaaatc tgattgatgt
     8041 actgaagata agcttgtaag aagccgatcg tcacaccacc tgatagaaca agcatagcac
     8101 cgaataccgc acttatgatg aaggcgagtt gattaatgaa acgaatggcc ggatagatag
     8161 cacagttcat aaattgagca cgtttaaaag cgttgtattg ctttttattt acttcgtgta
     8221 tagcagcttc tgcatgagct tgctgattga atgttttgac cactagatta ccagctaaga
     8281 tttcttccat tcgattattt aaaacaccca gttcatactg attctgttca gaaatctgtt
     8341 tgtttttgtt cgcaatctta gttgtaacaa ttgaactgcc aacgatcagc aataagacga
     8401 gcaaagtaag cttgctttcg atataaaaca gcatcagacc agcagcagct attgttacga
     8461 cagaagtaaa aaattgattg atacctgtca ataacacttg agaaagttga ttgagaccgg
     8521 tagttgtccg actaatgatg tcacctactt gatgattgtc aaaaaacgac atcggtaatg
     8581 ttttgaattt ttttgtgatt tcttttcgca ccctcaatgt gatcttttcg cttaacgctg
     8641 ccattgtccg ttcctgaata aatgaagtga tgctgcttaa gactgaacag ccgatcagca
     8701 ataagacagg gaacaacaat gcttgtttga tatcactgaa agaaagctgt ccaatacctg
     8761 tttttcttat ttctgataag agatcatcga tcccgattcc catgatcaag ggcatggcta
     8821 cgactaatag gttcccgaat aaactgcata gaaacaatcc gtaaaaatat ttatgttcag
     8881 gtttgatcat tttccaaaaa cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca
     8941 tcgtatccct tctttctata ttccttgtga ttttgcaaac tcctgataag tttggcaatt
     9001 agacagtagt tcttcatgag taccttgtcc aacgattttt ccttcatcta aaacaatgat
     9061 gttatctgct tgttggattg tactgaggcg ttgagcaaca atcacgagag ttttctccga
     9121 catgtgttta tgaagggctg ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa
     9181 actatcatcg aatacataga tctctgctgg tttgatcaac gctcgagcaa tacatatccg
     9241 ctgtttctgg ccccctgaat aattagaacc gccttgagca acaaagctgt ccaatccgtc
     9301 aggaagggtg tccactagtt cttctaattg agcgatcttg atcgctttag acaactcttc
     9361 tgttgaggca tattcatttc ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa
     9421 agccttctga gggacataac tgatgtggga gcggatctgt tcttgagtca tttgagtaag
     9481 aggaatacca ccaagggtta cttttcctat cgtagggtca ctaagtctca agagaagttt
     9541 cgccaaacta ctttttccag atccggtccc gccaacgatc gctgttgttt ttcctttagg
     9601 tattgtgaaa tcaatatctt ctagaactgg atcagagatt tcagtatatt taaatgtgac
     9661 atgttcaaaa gcagctgcat ttgtctgatc aaaggttggt tcagctactg ttttgatcgt
     9721 ctcttcctca atttcgatct ctgcttgcag tatttcttct atccgtcgta atgaggcaaa
     9781 cgatttaggg ataatgacca ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat
     9841 agagtattcg actacagcag tcacgacacc gatttgtaat gctccttctg ctacaagatt
     9901 tccgccaaac cacaaaatag aagcataaac gattcccatg agcaaaaaag caattggagt
     9961 gacccaagca aatattcggt tcactttgat catttgatta gcatagtcag caaagctctt
    10021 attcgttcgc ttttcttcat atgattgatt atcaaaggca cggatcatat taatcccagt
    10081 aaagaattct ctcaatgtga tggtgatccg atccattttt ggctggatac tcaaagataa
    10141 aggtgcccct cgtttcatca gtcgataaac aatccataca taaaaaacaa cagagatcaa
    10201 tgtaacccat gttagtaata gagaataacg aaacatcatg agaatggtga aaacgcaaat
    10261 aaccggtgct ggtaagatca tttggcaaaa taaaacaatc atttgctgga cattatcgac
    10321 atcattcgtc attcttgtca aaagagatcc agaactaaaa cgactgatat ccttcattga
    10381 cagctgttgg aatttacgga aatatttctc ccgcgtttgg aatccaaacc tagcggaagc
    10441 caatgctgaa aagtaactag aggcaactgc agcgattgtt cctagaaacg ctacaagcag
    10501 catttcaact ccaatttttt ttataactga ggcttcgcca cttgctatac ctgtgtcgat
    10561 cagctttgct actaattttg gtacacctaa tgtaccggcg atttctaaac agatcatcac
    10621 aaaggtaagg agagcgagtc ctttgttttt ctgtaagaat gtttttaaga tagacatatt
    10681 attcctcctt ttttcctgat acttttcgat tctatacctt caagtaactt gaaggtcaat
    10741 agaaaaaaga ataaactgaa aaagaggtga gaaaaaatga aaaaaatgta tacgattggc
    10801 caagtatcgg aactatttga tttgccaaaa tcaacgatcc gctattggga tgaacaagga
    10861 ttaatccgtt caagtagaca agaagagaat gattatcgat tgtttgatat cgatgatatt
    10921 tttatgcttt atgatatcgt tttttataga aaattagata taccgatcaa acagatgaag
    10981 aatttatatg gaaaaacatt gacggagtta tatgagacac ttgatgaaac agaaagaaga
    11041 atccataaag aactggtggt gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa
    11101 cagttgaaat tgatgatcga tacaaacgag gaagaatttc ctgtagaaga gatccctttt
    11161 gactgtatga tctcaacgga gtttgaagat attgtggaaa tcaaaaaatt tctacctaac
    11221 tattcaagct ttgggatgat gagtatgcca gcttccagtt cacagacgat gtatggattt
    11281 tttatcgacc ctagtgaagt acatttattt actcaagacg tgatttggga gaaaaaagat
    11341 acggctgttt atcgtcgatt cttattgaaa tccgagatga accatgcgga acgtaataat
    11401 attttagaaa tcagagaaag aatgtatgaa aaaggatgga aaacaggaga agtgatcggc
    11461 cagtatctat tgacgaatac ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg
    11521 atagaaatga aaaaatagga aatatgtgtt taagataact cgaaaatgat ggttgtttta
    11581 gttataaaga aaaattttct ggttcgatta cgggtttaat gtgggggatt attagtcgtg
    11641 gatagataga attttagttt tttagaggaa atatgtcttt aaaaatggaa tggttgcaat
    11701 tcttagcgat atatacgatg taattgtcct cttaaaaaag tatggaaaaa taattgtatt
    11761 gggtgttatg tataattcat ggtgaactga gactaatgag aagaaaaatt catattgtta
    11821 tgagtgagat tgtatcagat gagaatgata aattaaatag gtgcataaaa gcattacact
    11881 taaaatacat tgttgcttac attattacaa tatgttaata aggcaaaaat aaatagatgg
    11941 attgaataaa catatttttt aatcaaatag tccctactgt gactttcctc agctatcggt
    12001 gatactaccg atagcatttt tttgcttata aaataattat aagtatttag agatttttct
    12061 tctaacattc ttcaaacaag aaaaatcaca aaggttattg ttatttttat atattctttt
    12121 taagaatgat tgttttttta tagaaatttt aaaaaataaa acacgttatt gatggatagt
    12181 tagtttgtta tttggtaata ttatagaagt tgactggtag tgatttattt ttcgtcgttt
    12241 tataaaaatg ttattttttg gaaaaattca caaatatgta acgaatgttt ttctg
//
LOCUS       DASJST010000088        12226 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJST010000088 DASJST010000000
VERSION     DASJST010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.088"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..566
                     /locus_tag="U4B05_001929"
     CDS             <1..566
                     /locus_tag="U4B05_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEN2051059.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /locus_tag="U4B05_001930"
     CDS             967..1572
                     /locus_tag="U4B05_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN2051060.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="U4B05_001931"
     CDS             1588..2160
                     /locus_tag="U4B05_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2051061.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="U4B05_001932"
     CDS             complement(2412..2669)
                     /locus_tag="U4B05_001932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051062.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="U4B05_001933"
     CDS             complement(2795..3610)
                     /locus_tag="U4B05_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051063.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="U4B05_001934"
     CDS             4127..5452
                     /locus_tag="U4B05_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2051064.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="U4B05_001935"
     CDS             5445..5795
                     /locus_tag="U4B05_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051065.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="U4B05_001936"
     CDS             5792..5962
                     /locus_tag="U4B05_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051066.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /locus_tag="U4B05_001937"
     CDS             complement(6866..7141)
                     /locus_tag="U4B05_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN2051067.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="U4B05_001938"
     CDS             complement(7113..8066)
                     /locus_tag="U4B05_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2051068.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="U4B05_001939"
     CDS             8678..10171
                     /locus_tag="U4B05_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2051069.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="U4B05_001940"
     CDS             10306..10602
                     /locus_tag="U4B05_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN2051070.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="U4B05_001941"
     CDS             10703..11554
                     /locus_tag="U4B05_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051071.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11858
                     /locus_tag="U4B05_001942"
                     /pseudo
     CDS             11575..11858
                     /locus_tag="U4B05_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4205 a         1873 c         2286 g         3862 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca cctggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaacggttc tgttgcaaag ttttaaatct actatcaaat
    12181 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatc
//
LOCUS       DASJST010000089        12204 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJST010000089 DASJST010000000
VERSION     DASJST010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.089"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(166..330)
                     /locus_tag="U4B05_001943"
     CDS             complement(166..330)
                     /locus_tag="U4B05_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051072.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            625..1953
                     /locus_tag="U4B05_001944"
     CDS             625..1953
                     /locus_tag="U4B05_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN2051073.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2155..2988
                     /locus_tag="U4B05_001945"
     CDS             2155..2988
                     /locus_tag="U4B05_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN2051074.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3004..3741
                     /locus_tag="U4B05_001946"
     CDS             3004..3741
                     /locus_tag="U4B05_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN2051075.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3896..4864)
                     /locus_tag="U4B05_001947"
     CDS             complement(3896..4864)
                     /locus_tag="U4B05_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN2051076.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5096..5929
                     /locus_tag="U4B05_001948"
     CDS             5096..5929
                     /locus_tag="U4B05_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN2051077.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5963..6802
                     /locus_tag="U4B05_001949"
     CDS             5963..6802
                     /locus_tag="U4B05_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2051078.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6828..7388
                     /locus_tag="U4B05_001950"
     CDS             6828..7388
                     /locus_tag="U4B05_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2051079.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7587..9308
                     /locus_tag="U4B05_001951"
     CDS             7587..9308
                     /locus_tag="U4B05_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN2051080.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9473..10615
                     /locus_tag="U4B05_001952"
     CDS             9473..10615
                     /locus_tag="U4B05_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2051081.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10631..11842
                     /gene="thiI"
                     /locus_tag="U4B05_001953"
     CDS             10631..11842
                     /gene="thiI"
                     /locus_tag="U4B05_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN2051082.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4155 a         2049 c         2537 g         3463 t
ORIGIN      
        1 gtcgtaggtt cgagtcctac tgccggagta aataaagctt atgcaaagaa aaagaaatcc
       61 aatcacttgg gtttcttttt cttttattat tatgatgaat caagctttct gtttttgtca
      121 cgtatgttgt aatctttacg aaagagtaga gttggctact cataatcaac catacttccc
      181 atcatcgcta aatacgaaat aaatcagcat tcctacacca atcaaagcac aagctgcacc
      241 aagaaaaggg atgacattcc caaagttatc tgtattcata agtatgccac cacccacaaa
      301 caaaaagccg ccagcaatca ggtttttcat tttattcatc tcctttctta cgttatagta
      361 tttccttctt tcaatgagta ttttgaaagc tatcttattg tatgatgttc ttctaggcct
      421 cttctttatt atacaggcca ttttcgttat ctcatagaaa tattcctttt tatctgaaaa
      481 ctattgtata ccgtatgaca tttttcttca tgaagttgta agtatattct cattcaaatg
      541 aaaatttcat catagaagag attgtatttc taaattctta agagtaaaat acgaagtata
      601 acttggattt ggaaggatga ttggatgaaa gctattacgc ctgatatgac aacaaaattt
      661 aacaaacagt ttcaagcaaa tatcaaacag caggcattgc agcgggcagt tgtgaagaac
      721 ggaattactg cttctgctga aaatgtttca gcacatgtgg agaatacacc ggtattttct
      781 atagatttgg ctacggggaa agtggctaat caaatgcaat cggggcgttg ctggatgttt
      841 gctgcactga acacattccg tcataagatg ctgaatcaat tccatttaaa agagtttgaa
      901 ttatcacaaa attacacttt tttctgggat aaatatgaaa aggcaaatta tttctatgaa
      961 aacatcattg caacaggaaa agaggcaatc gatagccgga aagtagcttt cttactcgct
     1021 actcctcaac aagatggcgg ccaatgggat atgatcgttt cattattcca aaaatatggc
     1081 gttgtgccta aatcggtgat gccggaaagt gcgaatagtt caaattctcg agatttgaat
     1141 aactacttga acaaattact gcgtaaagat gcagtagtgt tacggaaaat ggttgctcaa
     1201 ggagaaacat tagacgctat tgaagaaaag aaagaagaaa tgctagaaag catttataac
     1261 ttcttagcta tctcattagg aacacctcca aaagaattcg attttgagta ccgcgatgaa
     1321 gaaaagaact atcatcttga ccgaggactg acaccacaaa ttttttatga taaatacatc
     1381 ggtgtaaaat tagatgatta tgttagcgtg atcaatgcac caacaaaaga caaaccattc
     1441 aatcgttctt atacagtaga gatgctagga aatgtcgttg gcgggaaaga agtcaaatat
     1501 ctgaatgttg atatggaaac tttcaaaaaa ttagcgattg ctcagcttga agaaggagaa
     1561 tctgtttggt ttggttgtga tgtaggtcaa tcttctacta gaactagtgg catcatggcc
     1621 ttagatgctt attcaatgga tgatctattt gatacggatt ttacaatgac taaagctgaa
     1681 cgtttagatt ttggagaaag tctgatgacc catgcgatgg tattgaccgg tgttgactta
     1741 gtagacgggc aatcaacaaa atggaaagtc gaaaatagct ggggcgaaaa agttggaaag
     1801 aatggcttct ttgttatgag tgatgcctgg atggacgaat atacgtacca aatcgttgta
     1861 agaaaagatt tattgacgaa agagcaatta aatgcgttca atgaagaacc aattgttcta
     1921 tctccgtggg atccaatggg tgctttagca tagacgagta tttcaatgaa gagtatgtag
     1981 taaagataaa aagagctggc ttaagccagc tctttttatc tttaaagtcc attccctgtt
     2041 ttttatggtc cctgtgctat aattctaaat aaaggataag gattggaaat aatcctttta
     2101 tcattaaaaa tatgatgacg aagtagaata aaattgggag taggggggaa agtcatggat
     2161 atccaaactg ttttatctga tctgaaatta aatggtaaag tgattccagt tgttggtggc
     2221 gatgttaatc agacctatcg tataaaaacc gaacatcgtg cttatttttt aaaaatccat
     2281 ccgaatgtaa aaaaaggatt ttttgaagca gaagtagacg gattaaaaga actgtcagca
     2341 ttcgtacgtg taccggatac gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta
     2401 atggaatgga tcgagccggg aaaaggggat caacgagatc ttgctgcagc tttagctaat
     2461 ctccatcagc aaacagcgcc acaatttggt tttcgtaaag ataactattt gggtacactt
     2521 gttcagaaaa attcttttga agaagattgg tggacttttt tctttaaaga tcggcttgaa
     2581 tcccagattt ctttggcaga agaaacgaat cgatggaatg tacagcgtca agaaaagtat
     2641 ctgcgattta aagaacgtgt attgaaatcg gttgaaccaa aaaagatcac tccacgcctc
     2701 ttgcacggag acctgtggag tggtaatgtg ttctttgacc agcaagggca tccagttttt
     2761 gtcgacccag ctgtttctta tgggaatcgg gaacaagata ttgcaatgag ccagttattc
     2821 ggtggtttcc gaccggaatt tttagatgct tatcaaacta tatttccatt agaaaaggga
     2881 tggaaagacc gactgccgat ttaccagtta tattatcttt tggcacattt gaatatgttt
     2941 ggagaaagtt acggttccca agtagatcaa ttactagaaa atttttaaga ggagggcgtg
     3001 tttttggcaa aaaaatatac caaaaagcat gaagtttcat attatgagtg tgacatcaac
     3061 caaacgatga catttccttc gatgttagga gtagttatca aaacgtctga agatcaaagc
     3121 gaccagttgg gaagaggcag tgaattcgtc aatagctttg gacttacttg ggtcatcacg
     3181 aattattcca tggagattaa tcgtctccct agagtcggtg aagtaatctc agtaacgact
     3241 caagccatgg agtacaataa gtttttctgt tatcggaact tctggatcca tgatgaagat
     3301 gggaaagaat tagtcaaaat agaatctgtt ttcgtattga tggattttgt gaatcgaaaa
     3361 atgagcagtg ttaatgaaga gatcattgct ccttttgaga gtgagaagat caagaaaatc
     3421 aagcgtcagg aaaaaattga aaagattgat tcgggcttca tgttgccata tcgtgttcgt
     3481 ttttatgata tcgatagtaa tcagcatgtt aataatgcga tgtattttaa ttggatcatt
     3541 gacgtattag gatatgactt tttgactacg catgttccag aaaaagtaat tattcgattt
     3601 gataaagaag tagagtacgg gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa
     3661 aatggtgtca aaacaaaaca tgaaatccga atgcaagaac agctgtattg tgaagcaaat
     3721 attatatgga aaaataaata aaaaagaggt tgtgacagaa gggggcaact tcaagaaata
     3781 agaagaaatt tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa
     3841 gaagctattt ttgtcacagc ctctcttttt aagtttagct attagataat gatgtttagt
     3901 tttggataaa cgaaatcaat tcttcacccg acaaaccata gtttacagca atcaatagtt
     3961 ttaaacgtgc tttttgacta ttaatactgt tacagaaaat aatccccatt ttttctagtt
     4021 cttttccccc gccttcataa tcataaacag gctctgcgat tccattgaaa caacgagaaa
     4081 caagtactac agggattttt tggacaagaa gcttttccaa agcagctaag gcttgtggag
     4141 ggagattccc cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg
     4201 cttccaataa gtccccttgc atcccagcat atgctttaac gatcgggata gtcccatcta
     4261 cagcggaaat gtccagtcgc tttgtttcta aaagttcctg caagaataaa attcgattct
     4321 tagttactaa gccgatggga cccaatgtag gtgttcggaa agtagcaaca tttgtggtat
     4381 gggtctttgt cacgtagcgt gcagaatgga tctcatcgtt catgaccacc aaaaccccct
     4441 tatctagcgc ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta taaaggccgt
     4501 cacttccaag ttcattactt gaacgcattg ccccagtcaa aacaattggt aatttgttgc
     4561 caatcgttgt atcaagaaaa aaagcagttt cttctaatgt atctgtaccg tgtgtgatca
     4621 ccactccgga tatacctgat gcataagcgg acaagatacg ttcttttaat accaacatgt
     4681 gagaaggcat gatgtgtggt gagggaagat tgaagatatc ttcaactaat aattctacat
     4741 gcttggggat tgtgatttct gcatctaata atgggtttgc cacatcagga ctaacaccgc
     4801 ccgtgagcat gttttctttc atggcgatcg ttccgccggt gtgtagtaca agtatttttt
     4861 tcattttaca gacttcctta cgaataattt tgcatagccg ttaaatctta ttgtatacta
     4921 tataaaagag ttttgctatc agtgaagaat aacgaatagc ctaacttatc ataaaaaagc
     4981 tgaccttatt aaaatggaaa agactcaacc ttgacctcct aatacaatgg agtatctggt
     5041 tgagtttctt ttacaagaaa gcagccaagg aaatcaatca cgaaggtggc cggaaatgat
     5101 cagaatcgga ttaacatcgt ttaaagaaca tgacaaatta actggtaaaa agaataacac
     5161 tctttatgaa tatgcagggt ttctaccctt agtggaatta gatactgcct attatggaat
     5221 tccccgaaag tcctcagttg agaattggat aagtgaagta cctgaatcat ttcgctttgt
     5281 agtcaaagtc tatcaaggaa tcagcggtca aggagactgg aagccttatt atcagaccga
     5341 agaagagatg attgcgcatt ttttagatgt tatgtcgcca atggtagatg ctggaaagat
     5401 gttttgcttt ttagtccaat tcccctcatc attcaaatgt acgaaagaaa atgtccagta
     5461 cttgcgcaaa cttagaaagt ggtttgaggg ttatcctgtt tctatagaat tgcgggatta
     5521 ttcttggtac agcgatgaat ttcttgaacg tacaagaaac tttatgtcta gagccgcttt
     5581 ttctttagcc atcatcgatg agccacagtt gctgaataca acgattcctt ttgatccatt
     5641 tgtcaccaat ccgaactttg ctctttttcg attccatggt aggaatgcgc aaggatggca
     5701 agcaaatgat aaagagtggc gtaaaaaaag aacgttatat cgttattcag aaaaagaact
     5761 gcaagaacta gcacatgaag tcaaagtagt tgattctaag acaaaagagg taggtgtgat
     5821 ttttaataat aattctggtg gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt
     5881 aaatatcaag ccagatggac tcaatccaac ccaaatggat ctgttttaag ggcttatttt
     5941 attcaagaaa ggggcgaagt ctatgatcaa agctattttt tttgatattg acggaacatt
     6001 ggtcaccaat cgttcaaaag cgttggaatc aacaaaacaa gcaattgaat atgctcggaa
     6061 aaacggcatt ttatgcggtg tagcaacggg gcgcagccca gtaaagatca aagaaatcat
     6121 tgatgaattg gaattagata tgtatgtcgt gtataatggt caactggttt ttactgcaga
     6181 tcgcacgatt atcgaccacc cttttgaaca aaaagtattg gaacatattg ttgaatttgc
     6241 agatgagaac catcgacaaa tcgtttttgg tgcgagaaat cggttagatg gatcaacgac
     6301 gatgctatta ggacaatcga tttttatcaa gcgactagtc agctttttac cgcgtaaatt
     6361 cccggtacgt ctgatgaaaa aaatcctcca ggtttttagc cctcaccgac aaaaggatcg
     6421 ttatgaaacg ttagagatat taaaagaacc aatttatcaa tgtatattgc tcagtccgga
     6481 gtcagaacag aaaaagttag aacatcgctt tcccgagtgt acgtttcagc gttcaaatac
     6541 gtatactgta gatatcatcc caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc
     6601 aaatgctgtc gaaattgaaa tggaagaaat tatggcattc ggcgatcatt acaatgatat
     6661 cgagatgctg aaaggtgtag gaattggagt ggccatgggg aatgctcaga tcgaagtaaa
     6721 acatgctgca gactttgtga ctcaatcaaa tgaagaagat gggatatacg aaggactgaa
     6781 acacttcggt ctgatttatt agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga
     6841 caccttttca aaaagtagaa acattccaca aaacttttga tccaagaaga ccagagatac
     6901 ccacggcttt tacagaaaat gaagctcata cacgtgctgc gttcaaaata gaagaacttg
     6961 ttgaatttgt tgcagctact tcacctaata aagaacaact gcatgaagct gttatgctgc
     7021 tgcacgaagc attagataaa gcagaagaaa aaatacttgg taaaacacag tggggaagtg
     7081 atccgctggt tgaacaagta gatgcactga cagatatcct ttactttact tatggttctt
     7141 tttcattact aggaatagtg cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg
     7201 ggaaattatt tccagatgga aaacctcatt atgaccctgt aacaaataaa gtgttgaaac
     7261 ctgaaaactg gcagatggaa tatgctccag aacctaaaat cgcggcagaa atccaacgtc
     7321 agattcaaga agcgattgct aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa
     7381 aagagtgatc agagtttcac tatttttgtt atctttatac gatggaaaaa caaaagattt
     7441 tcgtttaagt tagtgaatgc atgaaaatag tatgctataa tctagaatag atgtcttttg
     7501 cacgagagac gtattatttg gatttatgcc aagcagaacg ccatctgttg cataaaagat
     7561 gcatactgaa aacggaggaa ccataaatgg gaaataatgt tatcatcgga atcgtcattg
     7621 caatcattat agtcgccatt gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc
     7681 aagatcgatt ggatgcctta gaaaaacgca aggaagaatt atttgacttg ccagtgattg
     7741 aagaagtcga tgatgttaaa aaaatgcatc tggttgggca aagccaaaat acctttcgtg
     7801 aatggagtca aaaatggaat gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa
     7861 ttttcgaagt ggaaaacctc aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg
     7921 ttgaagcaga agctacaatg gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc
     7981 taaaggaatt gcgggaaacg gaagaacgca actcactagc tgttcagcag gcacttgacg
     8041 tctatgaaga actgaaagaa aaagtcagag aagacgagca tgaatacgga ccagctcttt
     8101 ttgaattgca aaaacagatc aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa
     8161 acacttctgg agaccctgtt gaagcccgcg aggttttgga taaagcagaa aaacatacgt
     8221 atgaagtgga agctctgatg aaaaaaattc caccgctgta tgaggagctc cataccactt
     8281 tccctgaaca acttgaagaa atcagtgata cttacgagaa attgatggaa gaacaatatg
     8341 tattcccaga agaagatctt gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt
     8401 cattagccaa tcttgaaaaa acagaagttg aaacggtcga atttgaaaac cgtgaaacag
     8461 ctgatttgat tgattcgtta tatgatatct tagaacggga aatggaagct cagagatatg
     8521 taaaaactaa ccaatcaacg attgcagagt atatcaaaca tacaacaaag aataaccgtc
     8581 aattattgat tgagctggat cacacatctc aaagctatac attgaaccac aatgaaattg
     8641 gccgtgtacg aggtttccaa accgaagtgg aagaaatgga acgtcaaaat gaacagatga
     8701 ttccacagat ccgtcaacac gaaattcctt attcagaagt gcgcacgttc tataaaacag
     8761 tcttcaaagt cttagaagat atcgaaaagc aacaagtaga gatcgacgac tcattacatg
     8821 aattgagaaa aggcgaaaaa gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc
     8881 gcagtttgaa acgttttgta gaaaaacaac gtttgcctgg cttgccgaat gattacctag
     8941 aattcttttt tgttgctact gaccgtattg aagaactaag tgttgtcttg aataaaattc
     9001 gagtaaacat ggaagaggtc aatcgtttag ttgctctatg tgaagaagat ctagaattat
     9061 tagataagaa gacacatgat ttagtagatg cagcagcatt aacggagcaa atgctccaat
     9121 atgcaaatcg ttatcgtcat acacacgaag aagtgagaga agcaattgat aaaagctact
     9181 atttattcaa taaagaatat cactatcaag aagcattaga cgaaatcggt acagcattag
     9241 agcgagtaga acctggtgct ttcaaacgca tcgaagattt ttattttaat catccagact
     9301 tagtataaag caaaaaagtt agtaaaccag caagagcaag acggattatt gtcttgttct
     9361 tgtctttttt tgtatatttg aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc
     9421 accttggaca accgttataa tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata
     9481 ttttgataac agtgcaacaa ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac
     9541 cagtcagcgg attattggga atccatcaag cttgcatgat ttaggcaatc aagctaaccg
     9601 gttgttgcag caagcaagaa agcagatcgc tgatttgatc catgtagaac cagaagaaat
     9661 ctattttact tcaggcggaa cagaaggaga taactgggta ttaaaaggaa cgatgatcga
     9721 aaaaagagaa tatgggaatc atatgatcat ttcaggagtc gaacatccgg ccgtcagcga
     9781 gacagcagaa caattaaaag aattaggaat cgaagtaagt atcgccccag tggataaacg
     9841 cggatttgta cgtgtcgacg aattgaaaga attaataaga aaagagacag tattagtctc
     9901 tgttatggct gtgaataatg aagtaggatc gattcagccc atccaggaaa tcagtgatct
     9961 tttggatgat tttcctaaaa ttcatttcca tgtagatgct gttcaggcga ttggaaaagt
    10021 accattctcg caatggttga cagaaagagt tgattttgca acattttcag ctcataaatt
    10081 ccatggccca cggggaactg gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt
    10141 actgacaggt ggagggcaag aaagaaatca gcgaagtggg acagaaaatg ttccaggtat
    10201 tgtggctatg gttaaagctt tacgtttatc attagagata aaggaacaaa aacctgagca
    10261 gacacaacgc ttaaaagtag ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat
    10321 tttttcagaa ggaactgact atgcaccgca tattttatgt tttgctctaa aaggaattcg
    10381 cggcgaagtt ctcgtccatg ccttagaaga aaaacaaata tttgtttcta ctactagtgc
    10441 ctgctcctca cgtaaacata tggcaagcag tacattgcac gcaatgcatg taccaaatga
    10501 tctggctaca tctgctgttc gaatcagctt agatgaaaac aattcgcttg cagaagtcga
    10561 gcaatttatg attattttca agcagcttta taagaaattt tctgcaatca attaaaagag
    10621 gagtgttctt gtgaaataca gtgaaattat ggtccgttat ggggaacttt cgacaaaagg
    10681 aaagaatcga aaaagcttta tcatgcaact agcacaaaat gtcagacaag ctttgaaaga
    10741 ctttccagaa atcaaaatcc atgctgatcg ggaccggatg catcttttat tgaatggcgc
    10801 agacgggcag ctagttattc caaaactagc aaaaatattt gggatccaaa atttctctcc
    10861 aagtattcgt gtagaaaaag atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa
    10921 agaaatttat actgggaaag aaacatttaa aattatcgct aaaagaagcg atcaccaatt
    10981 tgaattgaca agtaatgaat tgaatcaaac attaggtaat gctgtttttg atattttccc
    11041 gcatatccaa gttcaaatgc gtcaaccaga tattccatta cgtgtagaga ttcggcgtga
    11101 tggcgcttat ctatcgtatg aaacgatcaa gggagctgga ggattgcccg taggaacaag
    11161 cggaaaagga atgcttatgc tttctggggg gattgattct cctgtagcag ggtactttgc
    11221 catgaagcgg ggagtggaaa ttgaagctgt tcattttgct agtccgccgt atacaagtga
    11281 acaagcactg caaaaagcaa aagatctaac agccaaacta gcaccttatg tgggaacgat
    11341 ccattttatc gaagtgccat ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca
    11401 aggatattgg atgacaatta ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg
    11461 tattcgtcat ggtttagtca ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac
    11521 tttgcaaagt atggtggcta tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc
    11581 gacaatggat aaacttgaaa tcattgaagt agcagaacag atcgatacat ttaatctttc
    11641 tatccagcct tttgaagatt gctgtacgat ttttgctcct ccacagccaa aaacacgccc
    11701 tcaactggaa aaggtccatc aatatgaaga gcgtttagca atagatgaaa tgatccaacg
    11761 agcactagct ggcttgaaga tggaagagat acaagcagaa tcgtctaatg aaaaaactga
    11821 agaattttca gaattgctat aagaggttat gaaaatagtg ttttgaccag agtattagag
    11881 aaacaaaacg aaaaaatagc tccatgtatt ttaccgtttt tgaactaatg tcgaagatta
    11941 gctgattgaa taccatttat caagaagtta tggaaaagcc ggttcttttt tactaactga
    12001 aaaataaaat tttatttaga gatcaagcta cagttgataa atgatataaa aaagagaatt
    12061 tcccatgaaa tagcgaggtt gtgacagaag tgggcagctt caagaaataa gaaggaattt
    12121 acgaaaattg cttttcaaat ttttgtgaaa tttcggctta tttttcgaag aagttacttc
    12181 tggaacaccg tgtatcagag gttg
//
LOCUS       DASJST010000090        11858 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJST010000090 DASJST010000000
VERSION     DASJST010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.091"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(113..694)
                     /locus_tag="U4B05_001954"
     CDS             complement(113..694)
                     /locus_tag="U4B05_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2051083.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(877..1503)
                     /locus_tag="U4B05_001955"
     CDS             complement(877..1503)
                     /locus_tag="U4B05_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2051084.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1525..2856)
                     /locus_tag="U4B05_001956"
     CDS             complement(1525..2856)
                     /locus_tag="U4B05_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN2051085.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3011..3412)
                     /locus_tag="U4B05_001957"
     CDS             complement(3011..3412)
                     /locus_tag="U4B05_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2051086.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3498..3704)
                     /locus_tag="U4B05_001958"
     CDS             complement(3498..3704)
                     /locus_tag="U4B05_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051087.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3799..5301)
                     /locus_tag="U4B05_001959"
     CDS             complement(3799..5301)
                     /locus_tag="U4B05_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN2051088.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5319..5774)
                     /gene="argR"
                     /locus_tag="U4B05_001960"
     CDS             complement(5319..5774)
                     /gene="argR"
                     /locus_tag="U4B05_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2051089.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5872..6489)
                     /locus_tag="U4B05_001961"
     CDS             complement(5872..6489)
                     /locus_tag="U4B05_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN2051090.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6710..8083
                     /locus_tag="U4B05_001962"
     CDS             6710..8083
                     /locus_tag="U4B05_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2051091.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8158..9270
                     /locus_tag="U4B05_001963"
     CDS             8158..9270
                     /locus_tag="U4B05_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN2051092.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9342..10979)
                     /locus_tag="U4B05_001964"
     CDS             complement(9342..10979)
                     /locus_tag="U4B05_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN2051093.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11147..11755)
                     /locus_tag="U4B05_001965"
     CDS             complement(11147..11755)
                     /locus_tag="U4B05_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2051094.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3133 a         2497 c         2141 g         4087 t
ORIGIN      
        1 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
       61 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
      121 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
      181 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
      241 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
      301 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
      361 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
      421 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
      481 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
      541 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
      601 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
      661 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
      721 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
      781 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
      841 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
      901 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
      961 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac
     1021 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     1081 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     1141 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     1201 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     1261 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     1321 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     1381 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     1441 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     1501 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     1561 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     1621 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     1681 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     1741 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     1801 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     1861 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     1921 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     1981 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     2041 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     2101 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     2161 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     2221 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     2281 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     2341 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     2401 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     2461 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     2521 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     2581 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     2641 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     2701 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     2761 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     2821 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     2881 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     2941 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     3001 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     3061 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     3121 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     3181 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     3241 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     3301 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     3361 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     3421 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     3481 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     3541 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     3601 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     3661 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     3721 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     3781 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     3841 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     3901 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     3961 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     4021 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     4081 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     4141 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     4201 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     4261 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     4321 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     4381 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     4441 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     4501 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     4561 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     4621 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     4681 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     4741 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     4801 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     4861 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     4921 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     4981 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     5041 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     5101 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     5161 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     5221 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     5281 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     5341 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     5401 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     5461 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     5521 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     5581 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     5641 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     5701 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     5761 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     5821 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     5881 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     5941 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     6001 ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc
     6061 acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt
     6121 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     6181 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     6241 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     6301 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     6361 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg
     6421 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
     6481 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
     6541 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
     6601 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
     6661 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
     6721 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
     6781 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
     6841 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
     6901 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
     6961 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
     7021 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
     7081 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
     7141 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
     7201 gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt
     7261 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
     7321 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
     7381 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
     7441 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
     7501 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
     7561 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
     7621 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
     7681 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
     7741 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
     7801 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
     7861 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
     7921 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
     7981 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
     8041 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
     8101 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
     8161 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
     8221 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
     8281 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
     8341 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
     8401 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
     8461 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
     8521 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
     8581 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
     8641 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
     8701 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
     8761 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
     8821 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
     8881 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
     8941 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
     9001 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
     9061 acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc
     9121 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
     9181 ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc
     9241 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
     9301 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
     9361 aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca
     9421 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
     9481 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
     9541 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
     9601 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
     9661 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
     9721 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
     9781 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
     9841 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
     9901 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
     9961 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    10021 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    10081 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    10141 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    10201 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    10261 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    10321 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    10381 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    10441 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    10501 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    10561 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    10621 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    10681 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    10741 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    10801 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta
    10861 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    10921 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    10981 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    11041 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    11101 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    11161 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    11221 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    11281 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    11341 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    11401 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    11461 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    11521 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    11581 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    11641 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    11701 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    11761 actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata
    11821 tttcataggt aaaagaaaca tgacatttaa tgacaaaa
//
LOCUS       DASJST010000091        11680 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJST010000091 DASJST010000000
VERSION     DASJST010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.092"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            187..3414
                     /gene="fss3"
                     /locus_tag="U4B05_001966"
     CDS             187..3414
                     /gene="fss3"
                     /locus_tag="U4B05_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN2051095.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3526..3840
                     /locus_tag="U4B05_001967"
     CDS             3526..3840
                     /locus_tag="U4B05_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2051096.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3853..4227
                     /locus_tag="U4B05_001968"
     CDS             3853..4227
                     /locus_tag="U4B05_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2051097.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4228..4581
                     /locus_tag="U4B05_001969"
     CDS             4228..4581
                     /locus_tag="U4B05_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN2051098.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4650..5666
                     /locus_tag="U4B05_001970"
     CDS             4650..5666
                     /locus_tag="U4B05_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN2051099.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6260..8134
                     /gene="ltrA"
                     /locus_tag="U4B05_001971"
     CDS             6260..8134
                     /gene="ltrA"
                     /locus_tag="U4B05_001971"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2051100.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8260..8616
                     /locus_tag="U4B05_001972"
     CDS             8260..8616
                     /locus_tag="U4B05_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN2051101.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8661..9803
                     /gene="dcm"
                     /locus_tag="U4B05_001973"
     CDS             8661..9803
                     /gene="dcm"
                     /locus_tag="U4B05_001973"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEN2051102.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9793..10082
                     /locus_tag="U4B05_001974"
                     /pseudo
     CDS             9793..10082
                     /locus_tag="U4B05_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10338..11522
                     /locus_tag="U4B05_001975"
     CDS             10338..11522
                     /locus_tag="U4B05_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2051103.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11519..11632
                     /locus_tag="U4B05_001976"
     CDS             11519..11632
                     /locus_tag="U4B05_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN2051104.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3915 a         1996 c         2587 g         3182 t
ORIGIN      
        1 gtttgtgttc taaaatgtgt tctaatttaa aggcagtata tttcgtattt tatattaaat
       61 ttttaggctc attcattctc aaatccattc tatttagtca ataaatattt taaaaagact
      121 ttataaacaa agatgttcaa gcgagcatct tatttttttg tgcctaattt gaaaggagaa
      181 catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt gagtttggct
      241 gttcaatcaa ttgtaggagc aattgctcct acgattgctt ttgctgatga aattacccat
      301 ccacaaacgg taaccgtaga actagattta gcacatcaat atgctgttga aggaactttt
      361 agtgatggtc gtccaatgtc agaagtcact gttccgcatt atgcagtgta caacggcgtt
      421 aaacaagata ttttctgtgt tgaaccagga gtcccgattt acaatgagtt cactcctgga
      481 tatgagaaga acccgttacc agatatgtcc gataaagcca aattagtttc cgttttatgg
      541 aaaaaagtag gtacagatgc agatactcat attgtggcac agaagatgat ctggcaagaa
      601 gttaatgggt atacccttca ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca
      661 gccattgagg caaaaatcaa tcaagccatt gccgattatc agaagaaacc aagcttccat
      721 aatagtacgg ctaaaacagt attagggcaa tcgactactg tgacggatac gaataatctg
      781 aatttgtcag agttcgatga ggttgtggaa aataccgcaa acattgatta tcgtgtgaat
      841 ggcaatcagt tgataatcag cccaaatgcc aattcaaaag aaagtggtgt gttgacgctt
      901 aagaaatctg ctggtaccgg aacacctgtc gcctataaaa aggctggcca acagacttta
      961 atggccgggg cgatcgataa gccgaacact tatacggtca aaattgatgt ggaaatggag
     1021 ggttctttga agattaaaaa agttgataaa gaatcgggtg atattatacc aggaacggtt
     1081 ttccatttag actttggaaa aactttaccc gcaaaagacg tgaccaccga taaagaaggc
     1141 attgcgacct tagatgagat tcctcatggt acaaaggtaa ccattactga aaaatcggtg
     1201 ccggcgcctt atacgattga taccacaccc atgactgcca caattaaagc aggtgaaacc
     1261 atttcagtga cttcaaaaaa tacgcgagaa aaaggtcaaa ttattctgga taaaaccgga
     1321 attgaaacag ggagtgatct ttggaacgat aattactctt tagcgggcaa tacgtttgcc
     1381 attcgcaagg atagcccaac tggtgaaatc gtccaagaaa tgaccacgga tgaaaaaggt
     1441 catgcagaaa caccaaaaga gattgccaat gcgttggaat tgggaaccta ctatgtgact
     1501 gaaaccaagg ctagtcatgg ctttgtgaat actttcaaac cagtaaaagt cgaattgaat
     1561 tatgtcaatc aaacagtggc tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatt
     1621 actggtgaaa ccactttgac aaaagaagac aaagataccg gtcatgaaac tcaagggaaa
     1681 gctgagttta aaagagctga atacaccttg tttactgtta aggatggaaa agcggtcaaa
     1741 tggaccgaga actttaaacc agaattgttg aagggaacga aagcttctga tgaaaccgta
     1801 actttgactt tagatgaaaa gaatcaagtt gccgttaaac acttagcgat taatgagtat
     1861 tactggcaag aaaccaaagc gcctgaagga tataccttgg atgaaacaaa gtatcctgta
     1921 tccatcaaaa aggtggatga tagcgaaaaa aatgctgtga ttactcgaga tgttgcggca
     1981 aaagaacaag tgattcgctt tggctttgat ttctttaaat ttgctggatc aactactggc
     2041 actgccgaaa ctggcttcaa tgatctaacc tttaaagtgt cgccattgga aggaaccaat
     2101 gaaatcaccg gtgccgaaga tgaagcgacc acagcttata acgagcaatt agggtttgat
     2161 ggttatggca agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaagcc
     2221 ccagaaggtt ttcaaaagat tacgccatta gaaatccgtt ctacttttaa agaaaataaa
     2281 gaggattata ccaagagtga ttatatcttt actattaccg aaaaagggca aaagcaaccg
     2341 attaaaacgg taacggtccc ttatgggaat ctaaccaatg agaatttctc agtcagtttg
     2401 aaccgtttga tgctttatga tttgcctgag gaagaagata gcttaacttc tcttgcgact
     2461 tggaaagaag gagataaaga attgactact cttgattcta ccgagctagt cgataaattg
     2521 agctataatc tacatgaaat caaagaagac tggtatatcg tagctcaagc catcgatgta
     2581 gatgctacaa aagctgccca agaaaaagat aaaaaagcca aaccggtggc gattgcggaa
     2641 acaaccgcaa ctttagcgaa taaagagaaa acaggaactt gggagattct gcataaatta
     2701 actgtcgaac aagttttaga caagaccatt gtcttattca actatgtgta tgaaagcaaa
     2761 gaagcctatg aagctggtga tgagccggta gcgaaggatg ctagcttgaa caatcaagcg
     2821 caaactgtta attgtatggt ggaacgtcat gtttccatcc aaaccaaagc gcacttagaa
     2881 gaaggttctc agacctttac tcatggtgac gtggtagata tgtttgatga tgtatccatt
     2941 acccatgatg tactagatgg ctcaaaagaa gcttttgaaa caattctgta cgctttattt
     3001 ccagatggta caagtaaaga aatttggaaa tctggcaaga ttgattacga agtgaatgac
     3061 aaagaattta ccaaaaccgt acttgcggaa aaagtggata caagtaaata tccagaaggt
     3121 acaagtttta ccttcaaaga aattaactat gacaaagatg ggaatatcaa tggaaaacac
     3181 aatgaagatt tgaaagaaaa atctcaaact ttaacgccca aaaaagttcc gacaacacca
     3241 agtacaccag aacaatctga aatgccaact gttccaagtg gctctcaaga atctagtcct
     3301 acagtgaagg cattccctca aaccggggag aaaacttcca atgtgctact gttcattggc
     3361 tttagcttga tctttgcgac ggctggctat tatttttgga atcgtcggaa ctaaggtgat
     3421 aagatgaaac atgtaaatag tagaaaacaa tacgcgcgac cgcctcctaa aaaagtggtc
     3481 gcgctatttt tgtattcaaa actaacaatg aaaagaggaa aaaatatgga attgaaattt
     3541 gttgtaccag atatggtcga gacgttcgga aaactaacat acggtggtga aggtgaagtt
     3601 ctatcggaag ggtatggtcg aaatactaca attgttggtc gaagttacca tctgttttca
     3661 aataaacaac gggccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat
     3721 tttgaccaag atcaaccact aaaagctgta aatccccact taattgccaa aggctatgaa
     3781 attgagaatc gtgggtttac cgattacgtg ttgtatgttg atgatttagt gaaagcatag
     3841 gaggaaaaga agatgagatt aacagaaggt attgtcgtag attcaggatt aacctttggc
     3901 aagttgcgtt tttccgcatt acgtcgagaa gtacgcaagc aaaatgagga cggaacggtt
     3961 agtaacgagg taaaagaacg gacctataat ttgaaatcct ccgcgcaagg tcgaatgatc
     4021 caagtcagta ttccagcaaa tgtaccctta cgcgagtttg cttatgatgc agaggtagag
     4081 ctggtcaatc caatcgtgga tacggtcgcc aattatgttt ttcgggaagg aaccaccgtt
     4141 aattggttta tcaaggcaga tgatttagtc ttgaaacgac aactgaatca aggaaaacct
     4201 acgaaccaaa atgaaggaaa gaaataggtg aagaatatgg aaattaatgt tgaacatatt
     4261 ttagattgtc tcgatcaatt tggcaaaggt gagctaacgg aggaacaact cactaacgca
     4321 ttaacctatg atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac
     4381 gatgccaaag aagactttga tgtactggat tggttagggg aagaagagtc tttctttatg
     4441 gttgtcgagg taaatgaaag tttatgtcgg caggcagaat ctgttttgga agaaatcgat
     4501 gtggagatgc cggatgccat cgaaagattt ttaaaacaat tagtggaaac gaaacaactt
     4561 cccgtagtag taaatgacta gcataacttt ttgaaattgc ccaatgaaat cctattgggc
     4621 tttttcgact ggaggtggaa accatcaaaa tgtacaaagg acatcgcata cgcgcaggag
     4681 atcaacactt gatttatcac tttgttcttg gctggctaat ctttttgttc attggttgga
     4741 tgagcgtctt ttatttacaa gaacttagac aatttgatat ttctaaggta tcattttcca
     4801 ctagtgaaat tggctggtct atgaaggatg taatctgctt gctaggaagt ctcgctcttt
     4861 caggaagcat gatgttactt tatatacact tttttcagga tcattggcga agtctttggc
     4921 atcgacaaaa gttagctcgg atgattctag aaaaccattg gtatgaagta aagcagactc
     4981 aaggtgaagg ttttttcaaa gatttgaata gtagttggac caaagagagt atcagttact
     5041 tccccaaaat ttattatcga atgaaagatg gcttactttc tattcgcgtt caaatttcac
     5101 tgggaaaata tcaggatcag ctattgaagt tggaaaataa gttagaaagc ggactgtatt
     5161 gtgagttagt agaaaaagaa ctcaaagact cctatgtgga atacaccttg ttatacgata
     5221 tgattgccaa tcgaattggg atagaagaaa tagtggcaga aaatggggcg ttgcgattga
     5281 tgaaaaatca agtatgggcg tatgattcct tgccgcatat gttaattgct ggcggcacag
     5341 gtggtgggaa gacctatttc ttactcacca ttattcaagc actattgaag tcagatgcag
     5401 aattgtttat cctcgatccc aaaaatgcgg acctcgccga tttaggtacg ataatgcctc
     5461 acgtatactc ccaaaaagaa gaaatttctg aatgtgtgga agatttttac gaacgcatga
     5521 tagctcgtag taggtcaatg aaagaaatgc ccaactacaa aacaggagag aattatgcgt
     5581 atctcggact tccgcccaac tttttaatct ttgatgaata cgtggcttat atgggtgcga
     5641 atcgttttcc tacaagcatc gaatagatgt gaaatggaag cctattgaga ttatcaatag
     5701 tgacaactaa tgttttgaga gatggaatga aagccttcaa gttgagctga aacatccccg
     5761 ctaatactcc tacatgctct aatcaatgaa gtagattggt tttggtatga agctaggtga
     5821 agtcgtctga aatctgccta agtgcttgag cataaggctg actatagggc gggcggctga
     5881 aaaagataac tatggtaaga atgtctgaaa atgactgacg aatctgcgaa tgtacgggtc
     5941 taaaaggctg atcttattga aagataggaa catacggacg tatggtgaga taccgacaag
     6001 taaggttcca tgatatgaaa atcgggatag ctaacgaaga agctataatt tcacaggctc
     6061 atagtagata cctaaggtta tacatgatag ctaaaaacat tggaacgatg taagcagaaa
     6121 actatcaatt aaatgactac ttctagatag gtgcatataa gtaataacga aattgctttg
     6181 tttctgtgaa agtaagggca cagtaccgat gaagcgtcta acaaatgtgg agggatagcc
     6241 ctaagtctat ttaataacaa tgaatgttga aacagatgaa gttcaaggat ctggtaagaa
     6301 cgtgggaaaa tccgttatgg aggtgccacg cttgaacaca aaaatacgtt actgggaata
     6361 ttatgatcta caagaaacat tcgatcagct atttactcaa agtagaaacg ggaaaaagtt
     6421 ttatcaatta tatgagttaa tcatttctga aaataatatt cttttagcat accgtactat
     6481 caaggcaaac aaggggtctt cgacaccagg aacggattcc ttcacaattg ataactataa
     6541 ggaaatgaac caagcagagt ttatccatct tattctcagt catttagaaa attacaaacc
     6601 aaaatccata aagcgagtaa tgatacctaa acccaatggt gagaaacgcc cattaggaat
     6661 tccatgcatg attgatcgaa tgatccaaca gatgttcaag cagatactgg agccaatatg
     6721 tgaagctaag ttttatgaac atagctacgg tttcagacca ttgagaagtg caaagcatgc
     6781 tctaggtcgc attatgtatc tcataaacat cagtaaaatg cactatgcag tagacattga
     6841 tatcaaaggt ttctttgata atgtaaacca ccgtttactg ataaaacaat tatggaatat
     6901 aggaatttgt gacaaacaag tcttagctat tctttcaaaa tctctgaaat cacccattca
     6961 aggagaaggt atcccgagca aaggaacgat ccaaggaggt atcatttctc cactattatc
     7021 taatgttgtt ctaaacgact tagaccattg ggtctcaaag cagtggcata cctttgaaac
     7081 aaagtatccc tatactaaag gttacaataa atttcgtgct ctaagagata caaatctaaa
     7141 gcaaggctat attgtcaggt acgcagatga ctttaaaatc atgactaatg attatccgac
     7201 agcattaaaa tggttccacg ctgtcaagct ttacctaaaa gacagactta aattagatat
     7261 ttccaatgag aagtctaaaa tcgtcaattt gcgtaaatgt aagtcggaat ttttaggatt
     7321 cgctatttgc gtgaaacaaa aagggaaaaa atgggtttgt aattcacgta tttctaacaa
     7381 aaaaaaggat cagattaaag aagagattaa acaaagaatc aaagatattc aaaagagtcc
     7441 tacagcacaa aatgctctcc tgtttaatgc tctcgtgctc ggtgttcata attatttcag
     7501 gtatgccact catattaatt tagatttgaa taggatgaac tacgttcttt cattcactac
     7561 ttacaaccga ttatcaaatt gtagtacaag atgttacccc aacaaagctt ccccaactta
     7621 caaaaagttt tactcaacaa cgatgaaaac gtataaagta gctggagtct atttgtaccc
     7681 tctttgcgac gtatctacaa aaacaattta cggatttaat acggaggata caccgtttac
     7741 tccgtttggt cgtcaaagat tggagcacaa aagcctacag tctcttgtgt tccaagaatt
     7801 aagaaaattg atggaatcga aaataccgaa tagaacggtt gagtatttag atagtagaat
     7861 atctcgttac tcaatgaaat caggcaagtg tgagattaca aaacaatttt tgccagcaaa
     7921 agccgttcac tgtcatcact atttgccaaa aagtcttggt ggagacgaca aattcgataa
     7981 cttgagaatt attcataagg atatccatct tctgatccat acaacaaata aaatgataat
     8041 cgatcactac gtgcatgaac tgaaactggt gccagagcaa atagcaaaaa tcaaccttta
     8101 tcgaaagatg tgtaatctgc aaaacattca atagaaaata aatagatgga acgccgtatg
     8161 aggtgaaagt ctcacgtacg gtgtggagcg ggggaaaaga tggagattat atcaaattct
     8221 tacctatcgc tatagatgtt aacgacaaaa gaaagtgcgg tgattttgaa taagcttaaa
     8281 caaatcgtga tgttaggccg tcaatctggt ttctttctga ttctagcttg tcaaaggccg
     8341 gatgcgaaat atctaggaga tgggattcgc gatcagttta acttccgtgt ggctttgggt
     8401 cgtatgagtg agcttgggta ttccatgatg tttggagaag tcgataaaaa tttttttatg
     8461 aaacgcatca aaggacgagg ttatgtagat acagggggca gtgtgattag tgagttttat
     8521 acgccacttg taccaaaagg gtatgatttc ttaagggaaa ttagcaacat tgttggatta
     8581 accgttcata cggaaagaga aaataatagt gtgtagccta ggaatatcaa taatcctggg
     8641 cttttttatt gaggtgattt atgacatttt tagatttatt cgccgggatt ggtggctttc
     8701 gcttaggtat ggaacaagcc ggtcaccagt gtatcggttt ttgtgaaatt gatgaatttg
     8761 ctcggaggag ttataaagca atccatgata caaggaagga ggtggaaatg catgacatca
     8821 caagtgtatc agacgaattt gttcgatccc tcggaccagt ggacatcctt tgcggcggct
     8881 ttccgtgcca agctttttca attgcgggaa agcggcaagg attttccgat actcgaggta
     8941 ctttattctt tgaaatcgct cggttcgcca ctcttctcca acctaagttt ctattccttg
     9001 agaacgtccg gggattgctc aatcacgaag gaggggctac gttcgagacg atcctccgaa
     9061 cgttggatgg attggggtat gatgtggaat ggcaagtgct taactcaaag gcctacgttc
     9121 cccaaagccg tgagcggatc ttccttatcg gacattctcg agatgcatgt accgaacaag
     9181 tatttcctat cattggatcg tcttcaacat ctgatcaaaa catcagaaac ttggtaaata
     9241 tcaatccttc caatcgagga atgggtggcc aagtctatgg ttcagatagc gtagcaccaa
     9301 ccttgaccgg tgatgaagga attaaaattg ccttaccggt aaaggatggg atttctgtag
     9361 cgggtatgtt gtcgggaaat tttgaacaag gcaataggat ttatgaggta accggaacag
     9421 cccccacttt gtcaacgaaa caagggggaa cgaagattat gattcggaaa aatgaaacag
     9481 aatatcaaga ggtgaagcca ggggacagtg tgaacctggc ttttcctaat tccacaagtc
     9541 gccgaggtcg tctgggaaaa caaagtgtgc ataccttact aacaggagac caacaagcgg
     9601 tagtcacgga tcagtaccag attcgaaagt taacacctag ggagtgctgg cggcttcagg
     9661 gctttccaga ttgggctttt gatcgagctt ctcaagtcaa ttcagatagt cagttgtata
     9721 aacaggccgg aaactcagta acagttccgg tcatttttga tattgctaga agattaaagg
     9781 gggtaaaagc atatgattta tgacgagttg attggagaaa tctactggat gattggcaag
     9841 attcaggcag atccgaaatt ggaagaagaa ctgtaccggc tcaattttga tattcgcaaa
     9901 aacggggtga aagtccaggt gattcgtatg tagtggatga ggaaacggat gctcgaatgg
     9961 agctcaatca agtcatcggt gaattggaac gaattgcgga ccttgccaaa gaacctgata
    10021 tccgccaata cttgtttgag ataaaagcag agttggaaat tgagggtatc accgcggagt
    10081 aatagggaaa taacccactg ataggcactg accgcggggg atttgcgaag cagaaatcct
    10141 ccgtggtgct gtgcctgttc atggacggcg gcagccggcc atgatgtctc accccccgta
    10201 tctaacaggg gggtacaaaa acacaaaaaa caagcaagta accataaaaa atgcttgttg
    10261 tatcaaggat attaagtaat ttgtataggt gttaactcat ttggaaaagt taacaccttg
    10321 aatttggagg tgaaaacttg gcgcaacgaa atttagatta tcggttatta aaagaacgac
    10381 gcaacgaata tggcgtttca caaaataaat tagctactgc ttgcggactt agccgcccat
    10441 acctaaatca gatagaaaac ggtggcgtaa tcgcctctac aaagaccatg agaaaaattt
    10501 ttgatcaact ggaaagtttc aatcccgatt tacctttaac gttactattt gactatgtaa
    10561 ggattcgctt tcccacaacg gatgtgcgga aaattattca agaaattctc catttgaaat
    10621 tcgagtatat gcttcatgaa gattacgcct tttactccta tcaagaacaa tatgtcatgg
    10681 gcgatattgt agtgatgtta tctcatgaag aagataaagg tgtccttttg gaattaaaag
    10741 gtcgtggttg ccggcagttt gaaacttttt tactcgccca aaagcgtagc tggtacgact
    10801 tttttgaaga ttgtctaaaa gccggtggcg tgatgaaacg cttggattta gcaatcaatg
    10861 atcgagtggg gctactggat attcctgatt taacgaaaaa atgtcagaag gaagaatgta
    10921 tctccttgtt tcgtaccttc aaaagttacc gttccgggga actgttgaaa gctgatgaaa
    10981 aggatggtat gggcaatacc ttatatattg gcagcctcaa aagcgaggtt tatttttgtt
    11041 tgtacgaaaa agattatgaa caatatatca agttagggat tccattggac cagaccgaaa
    11101 cgaaaaatcg ttttgagatt cggttgaaaa atgaccgtgc ttattatgcg attcaagatt
    11161 tgttaaaagg tcgtagcatt gaaagtacca cgttttccat tatcaatcgg tatttgcgat
    11221 ttgcggataa agtagatgga aaacggcgaa ccaattggcc cttgaatgaa caatggggtc
    11281 gttttattgg acggaatcgg aaagagattc aattaacttc tgaaccgaaa ccgtatacca
    11341 ttgaacgaac attaaattgg ctaggtagac aagtcgcccc tacgtggaaa atggctaagg
    11401 agttggatcg attgaatcaa acaacgtata tccaggatat ggtacagaat gcgcgactct
    11461 cggaccggca taaaaagatt ttggagcaac aaagtatggc aatcgaaaac ttgattgtat
    11521 gaaaagagga attttgatgg aaattgtaaa agatatgtta ttggtacttg gtggcagtat
    11581 gcttggtgtt accgtgatgt gcttgatgaa tgcaagtgca gcagcggatt gagcaatgga
    11641 aaagaaagga aagtgacaaa atgaatttcg gacaaaattt
//
LOCUS       DASJST010000092        11563 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJST010000092 DASJST010000000
VERSION     DASJST010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.093"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(734..1912)
                     /locus_tag="U4B05_001977"
                     /pseudo
     CDS             complement(734..1912)
                     /locus_tag="U4B05_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1942..2352
                     /locus_tag="U4B05_001978"
     CDS             1942..2352
                     /locus_tag="U4B05_001978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051105.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2433..3053
                     /locus_tag="U4B05_001979"
     CDS             2433..3053
                     /locus_tag="U4B05_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051106.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3754..4038
                     /locus_tag="U4B05_001980"
     CDS             3754..4038
                     /locus_tag="U4B05_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051107.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4041..4958
                     /locus_tag="U4B05_001981"
     CDS             4041..4958
                     /locus_tag="U4B05_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2051108.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4951..5733
                     /locus_tag="U4B05_001982"
     CDS             4951..5733
                     /locus_tag="U4B05_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2051109.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5734..6414
                     /locus_tag="U4B05_001983"
     CDS             5734..6414
                     /locus_tag="U4B05_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2051110.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6407..7303
                     /locus_tag="U4B05_001984"
     CDS             6407..7303
                     /locus_tag="U4B05_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2051111.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            7498..9147
                     /locus_tag="U4B05_001985"
     CDS             7498..9147
                     /locus_tag="U4B05_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2051112.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9741..10676
                     /locus_tag="U4B05_001986"
     CDS             9741..10676
                     /locus_tag="U4B05_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051113.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            10669..11439
                     /locus_tag="U4B05_001987"
     CDS             10669..11439
                     /locus_tag="U4B05_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2051114.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3662 a         1920 c         2414 g         3567 t
ORIGIN      
        1 taatgaaaaa cgagaaggaa gatgacttta aagtagttta gtatgaaggt cgattgataa
       61 aacgatgtgg attaatgcta aataatgagt aatttttcaa taatacaaaa actgtgtaca
      121 gtattagaag tagagaaacg ctagcgtcga aaaaaagata tttttcgtga aaagtaatag
      181 gggctaaaaa gaaagtatac aatttattta tgtttctaag catataaaaa agagcgttga
      241 aaggaatatt tccccttcaa cgcttctttt ttagaagatt ttaatgacca tctcaacttc
      301 attttcaggt ttaggtaatt catggaagct tgtgtttatc gtgcttaatt gatcaactaa
      361 ccatgtttta ctttttgact cgtagacaaa tctaaaatta atatcattgt tctcgtgttg
      421 aagaggtttc tccaaataat ctggtgaata ttcatagtca acgtaacctg ctaatacaaa
      481 agaaagcagt ccattattta gtgtcagatt taacggatgg tcgtaagctt ttattttttt
      541 aatgtcatat tgtacatctt atgaagactt aatgactact tgtagtcaat agatttaaag
      601 tatttatttt ctcatttatg tatcaagaat aaatactgat ataataaaaa ttatatcaat
      661 gttttgatga agtaatttgg agggagcgtc aataattttg tgtaaataac tcgtccttct
      721 gcaaaataat gggttactca gtaaacattg aagctaatgt atccgtcact tgttggaagc
      781 ctttatggct tctatttaag aatttttgat tgtatgtatc aaagatggat actaggaaac
      841 gctctagtga ttcttcattt tgaaactgct cttttctgcg gctgtatttc ttgatttgtt
      901 tcttgaaaga ttcgattaaa ttagttgagt aaatgcttcg gcggatacca ggcggaaact
      961 cataaaaagt caataaatct tgatttttta tcagtgactg cgtcacttta ggataagttt
     1021 tttgctactt ctcaatcata ctccctaaaa aggtatttgc ctcttccttt gagctagctt
     1081 gataaacagc cttaaaatca tcacagattt cttttcgatc tttgacacgt actttatgag
     1141 caatattacg ggaaacgtga acacagcaat gttggtattt agctttagga taaatctggt
     1201 gaatcgtatc tttcatgcct tttaagccat cggtaataaa cagtaagaca tcatgaaccc
     1261 ctctggagta aatgtcctgt aacaactcat tccaaacgta ggtcgattca gtcggagcaa
     1321 tcgcataact tagtacttct ttagtgccgt cttctcgtat accaatggca atatagattg
     1381 cttctttgga gacggtttgc cgttttaaag gaatataagt agcatccatg aaaatagcga
     1441 catacttatc atttagagtt ctaactttaa atgcatttac ttcttcagtc agcactttag
     1501 tcatgttgga catggtttgg ggcgtgtagt gatgcccata cattttctca atcaagtctg
     1561 cgatttccga catggtaata ccttttcgga agaggtgaat gacggtctcc tctaagatat
     1621 cattcgtccg tctataagca ggaacagtct gttgcttgaa ctcgccgttg cggtcgcgcg
     1681 gaatctggag atgaagttcc ccgtactcgg tcttgaccgt acggtcatag gagccgttac
     1741 gagaattccc cgtgttaaaa ccaatgcgat cgtacttttc gtaatcgagg aaagccgtta
     1801 actccgtttt gagaagcgta ttgacagctt tttccaaatg gacacggaat aattcattca
     1861 aatcgccttt gttcgctaga gtctttagaa tttctgtagt aaaatcgttc atatggaagt
     1921 cctcttttct gtgaatgtgt tgtggtaact ctattctaca gaagggactt ccttttttct
     1981 atttacacaa aatattttac actctcgtca aatataaaaa ctagtacgaa attaatttgt
     2041 aaattattta agcttgataa aataagcaat atcgatgatg taaagtccaa gatattaaca
     2101 agaaaagtaa gcgaaccagt aaagctaacg gcacaagata aaaaaaatgg aattgttctt
     2161 tatggtgatg atgcaaagta taactataat tgggatacct cctcagaagc tgaggtatta
     2221 agtattccta ctaccgatac ttcaggggaa aaagtcacct ttaaaataaa ttttataacg
     2281 gcaatttcta agttggagta tgggaagcaa aaaaagagag ataacaatga tcaatatcta
     2341 cctaaaaaat aacgaattaa tgatgcatat gttaaagttg tttttttgaa ttaaacaact
     2401 caatttattc agttatatgt tcatcgcagc aagtgaacat taatcgagtt ataaaattaa
     2461 taggagctaa aaatataaat aaagttcgat cagaatttat ttttccagaa gattttttta
     2521 actggttttt ttatcaattc agtgaatgca agcgactctt aggtgagaat tattacttaa
     2581 aaggaatcgg cggatttatg gggaatgtgg ctgatgaaca aaatattata aaaggaatat
     2641 ctgaacaaac atcagagctt acagtaacta aagcatttat aagtaatggt gaaagtttga
     2701 aaaatgtaac tgcgcgaatt agaaaagaaa atgaagtaga cattgttttt ggaattgacc
     2761 atgaatgtaa ttcagtgata tatgtaacac aatcagcaaa tttaattctt tttgagaatg
     2821 gttgcaagga cgattttatg gtggtctatt tgtacgatat actaattcca aaacttcgtt
     2881 ccttatatat gtcttctgaa agtatttttg tgagtgaaca aaagggatct ttctcgaaaa
     2941 aaataggtat tgaagtaatt gaatcgatta tgtgtcataa tcatattacg ctagaagaat
     3001 tagaaatttt gatagggcaa aaaaataata acacgaaaat acaagtttct tagaaatcat
     3061 tttttattga gatatctaga taaatgattt attcttcaat caaccaactc gccaagctga
     3121 ggtaatgttt tgaggtaatg ataacataca actttttata ccctagtaaa actctataaa
     3181 caaagactta ttaatttcgc atgaaataca ttcctgtaaa atgcaataaa atagagtttt
     3241 gtttaaccag gtcttaagaa agcccgtaaa gcttcacagt tctcaaaacg ttaattgcga
     3301 ttatcgttcg agttcacgag ttacgagaat cgtactgggc gaagcaatgc tgagcgcagt
     3361 taccttaatc ctaacttttc tggcactttc cacaatttgg aaagtgcctt ttttgtgttt
     3421 ttagctatgt taataggtag gattcttggt ctataatatc attcgatcta taaacaaacg
     3481 ggaaaaagat aaagtatcat caaacaatat tatttatccg cttctgaggt tgtgacagaa
     3541 gtggtcagct tcaagaaata agaaggaatt tacaaaaatt gcttctcatg tttgataaat
     3601 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcggaa gaagggattg
     3661 tgaaaagtct tttgtcataa cctcttattt ggtagctaat tgtaaatccc aaacggcatg
     3721 tattattttt cataagtccg tttttaagga ttaatgtttt cttttgcttt ttatatggtc
     3781 tggcttcagc gagaagttcc aacgcatata caaggacgtc tgatgtcaac gataacaacg
     3841 attaccatgg tggccagtcc acttggatta gcactgtttg gtgcgctctt tgattggcag
     3901 acaactcaac cacgtctgcg agacagtctg atttttcttg aaattcaatt ggaaaattta
     3961 cactttcttt atacatttct ggctgctttc tttatacccg tcaactatac ttcacttatc
     4021 aataagaaag aaggataagt atggattttg gaaatgaaac ggtcttgaaa gcaactggta
     4081 taaccaaaaa atacggtgct gccaaagcat tagataaagt atcgatcgaa atcaaacgtg
     4141 gaatgatcta tggtttgatt ggggagaatg gagcgggtaa gtctaccttt atgagaacta
     4201 ttatgggttt gatctcgatt gatgaaggtt cgatcgaatt atttggaacg acggatttgc
     4261 aggcagcaag acgacgtatg ggtcaatcga ttgaaacccc tgctctttat ccagaattaa
     4321 ctgcaagaga taacctacgt atccaagcag cgaacggcgg tgtgtcggat agagaaatcg
     4381 aagacctgct gaaaatgatg cgattggaaa atacagggaa aaagaaagca aagaatttct
     4441 ctttaggtat gcgccagcgt ttagctattg caaatgcact gatcacgaat ccagaatttt
     4501 tgattttgga tgaaccgacg aatgggatgg atccagcagg gatggcagaa atgcgagaaa
     4561 tcatccaacg tttggtaaaa gaacggggca tcaccgtctt gctttctagc cacttgctag
     4621 atgaattgtc acagatcgcc actcactacg gtattttgca tgaaggacac ttgatcaaag
     4681 aattatcgaa agaagaattg gcacaagaat ctcgtcaatt tataaaaatt gacacttcgg
     4741 cgacggaaca agcagtaacg gttctcgact ctcttggtta tagggattat tttgtccaat
     4801 ctagccgtgt tatccagctt tttgaaggaa ttgaccaagt agcagccatc aatcaagcat
     4861 tggtagaggc aaaagttcct gtggatggta tccacctagt tgggcaaaaa ctggaagatt
     4921 atttcttaca attaactgga ggcaatccac atgtttaatc tattaactgc tgaaaaaata
     4981 aaagtatgga agagccgtaa aatgtgggtc tcactaggac tgatggtcgt acttccgctt
     5041 ttttattctt ggaacgaatg gtatatgcat acaaaatacg gcaatgaatt aaaccaagca
     5101 acggatacag tcatcaacgg tgcaacaggg attttaatgg tggaaaaaat ggctgcatgg
     5161 attcttctgg ctttcgcagc atttgcgtgc ttttatattg gggaagaatt tcaaaacggt
     5221 acgattcgca atacgttatc tttaggacgg aatcgaatga cttattatgt gtcgaaactt
     5281 ctcgttacgc ttttgatcac aacacttggc gtggttttga ttacggggct agccatgatt
     5341 gcttacacac ttgctttcgg atttggtgaa gttgagggaa tcaaaaacta tggaaattat
     5401 gttctaaaag tatttcctgt attgcttctg ctgatattag cgacattatc tgttccagtg
     5461 gctttgacat tcatcactag aagtaccagt gtcagtttgc ttctttcttt tctttatatc
     5521 atgggaacgg ccttcgttcc cggtgttttc gcaaaaatca aaggcttgga atttctaacg
     5581 gagtggttta cggaaacgtg gctcatgtat acggactttg cgcaacaagc aacttattcg
     5641 caagcaccga aaatgattct ggtcagtcta gtaacaatcg tggtatcatc agctctagga
     5701 atgtttattt tccaaaaatc tgacattaag taggtgttcc tgctggaaac tattttgatt
     5761 atcgaagacg atgaagctgt tcattcttta ctggaggagg ttcttgaaca gagatacaaa
     5821 cttcttgatg cttattcagg tacggaagga aaattattgt taagcacata tcctgtcgat
     5881 ttgatccttc tagatctcat gcttccaggg ctttcaggtg aagcattgtt agcagaaatc
     5941 cgacagacat cgaatgttcc gatcatcgtt ctttcagcaa aaagcgatca aagagataaa
     6001 gtttccttgt tggcagcagg agcggatgac tatgtgacga aaccatttga tatcgaagaa
     6061 ctgttgttac gaatcggcat ccaacttcgt catcagactt ctgtccaagt aaaagatctg
     6121 tcgcaacgca tctattacaa ggaaatctta ctggacaaag agtcgaggga tgtaaatgtg
     6181 aatggtgtgg ctgtccattt gacaggaaga gaattcgatc tgctgaagct ttttttagaa
     6241 catccgaaaa aagtattttc gcgagcaaat ctttatgaaa cagtttggca agaaccatat
     6301 tttgatagtg agaaaacagt gaatatgcat atttccaatc ttcgagggaa actagcaaag
     6361 gcacatgctg tgtatattca tacggtgtgg ggagtggggt ttagatttga ttaagtggct
     6421 gttgcttctc gttgttatct ttatgggaag ttttattttc ttcctgcaaa gggaaatcaa
     6481 gagattaact aagaaaatcg ccactctttc tgaacgacaa gaatatggtg gtcgtctttc
     6541 agtcgaattt cgagaaaaga atctaatgga cctagtagat gcactgaata catgggtgga
     6601 tgaaaatgaa caaaaaggac aagcatttca agaaatggaa gaaaacatca gactatcgat
     6661 cgtcggcctt tctcatgatc tcagaactcc tttgactgct attaacggtt atgtacaatt
     6721 actggaacag gtagaggata cggagaaacg ggagaagtat ttaacgatca tcaaacaatc
     6781 tgttgcaaga ttactcagta tgacggatca gttttatgat cttgctcgaa tagagacgaa
     6841 gcaaaaagaa atggatctcc atacgctttc tttttctaaa gaggtggaag aagtattttt
     6901 ctctttttat gaacaatttt ctgagaaaaa tatcgaggta gcgttcgctg aacactcgat
     6961 aaatacaact gtattagcag atccgctcat gttgctgcga gtgctgcaaa atatcataca
     7021 gaatttattg cgttatgcag atggacaagc aaaggtgaca ttttcaaaag aggattcatt
     7081 cattcgatta acagtggcca atcctatcaa accaggagcg caaacctctg ttgaaaaagt
     7141 cttccagcgt ttttataccg aaaataccag ccgcacaaat acggaatcta gcggaatcgg
     7201 gctttacctg tcaaaaaaat tggtggaagg aatgggagga gagatgaccg ctagattaga
     7261 cgggggcatc ttctccatct ccgtcaagtt gcggagggtt taattggctc taatttacct
     7321 gaagtgaatg acaaaaaatt gtatctcgtt ttaaagacat cttctttttt ggaaggtgtt
     7381 tttattttgg tgtgattgat acgaattagg taacgaagac agttattttt gtaccccctt
     7441 ttcaaatagc gcaacagtgc tatactaaaa ctaacaaatc aaggaacgga gagggatatg
     7501 ggccaaagag aatttatcgt tttaattatt attgcagttg ttattttatt agttgtttta
     7561 gacatttttt cacgtttgaa agtaaaggaa acagttcgta gtaattgggg aaaaattcct
     7621 tatcaaccga gatttgataa agaagaaagt ttgaaggatg cgtggctcac ggagaaaaaa
     7681 ttccgttcat gggatagtga aatcgacgat cttacttggt atgacttgga tatgttcgaa
     7741 gtatttgaag ggatcaacag tacctattct agtgtaggtt cagaagcgtt gtatcagcgt
     7801 ttacgtagct ttgattttgg agaagattat cagttagaaa aactaattgc tttttatcag
     7861 gaaaatcctc aactgagaga aagaatccaa tatcaattcg ctcgtttagg aaaaaaagat
     7921 cataattttg caaaacaata tctagcggat ggaaaaagta aaaagattgg acgcgtaggt
     7981 ttatttattt ttcttggctt gcttccgctg gtgggtgtag gtttgcttct tttagggcaa
     8041 gtactaggaa tctatctagc aatcggtggc ttagtattca atacggtcta ttatctagtg
     8101 aagaaacaaa cactagaaac agagctgaat agcatgagtt atttggtttc aacgatttca
     8161 gccgccaatc agttagcaaa aatgacgaca ccacttcaaa aagagctgaa acagcaaact
     8221 aaaccattaa gagacatccc taaattaggt ttttcatttc gaatgaagag cgggagtgaa
     8281 gcagaattga tttttgaata tgtcaacatc atgttcatgc tgccatttat ctcctatcat
     8341 ttcgttctca ctaaattaga aaagcattca actgaagcca tcaagctgtg ggaattgctg
     8401 ggagaattgg aagttgcagc agccgttttg aattatcgca cctatatgcc gatcacttgc
     8461 cgaccagagt ttactgaggg cggtgtgaca gcgaaagata tctatcatcc attgttaaaa
     8521 gaagcggtag ttaatccagt caactggcag aagaatacat tagtcacggg ttccaatgca
     8581 tcaggaaaat ctacgtatgt caaaagtata gccatcagct gtattctagc acagacgatc
     8641 caaacagcga ttgcagaaca gtttaccctg caacctggac atgtgttgac ttccatggca
     8701 atcgaagatg atttatttga aggcgacagt tattttgttt cagaaatcaa atcgatcaaa
     8761 cgtttgctgg accaagtggc ttccaaagaa cgctgctact gcttcgtaga tgagatttta
     8821 aaaggaacaa atacgatcga acggattgct tcttccgctt cagtcgttcg gtggcttgcg
     8881 gagtatccat ctttagcatt tgtcgccaca catgatatcg aattgacaga gattttaaag
     8941 aattattgtg agaatgtcca ctttgaagaa caagtaacag aaggaaaagg aatcagtttt
     9001 gatttcaaat taaaagaagg accttcaagg acccgaaatg cgattgcctt gctgaaagtt
     9061 ttgaattatc ctaaatccat agttgaacag gcccaaaaag aaagtttgtt gttcgatgaa
     9121 cagcgtcagt ggtatccgtt tgattaactt tatcaaacga agcatgatgc gtgggtccaa
     9181 aaagacgatt atcaaaagaa gtacattgat gccaaaaaag tggcagaatt caatgggaac
     9241 gagtctgctg tccagatgat cttggaagat ttagcaaaag acgatacaaa ataaaccacg
     9301 ctgtcttcct tctagcagct tttatcgaaa aaacttagca tcagcgacga gacgcacgtt
     9361 ccatggcaaa gttctccgta aaacaaacag cgaaaacaga aattgaaaat aactatttcc
     9421 attttattta tttcggttaa aataaaagaa aaaagctttc gataaaagga cggaggaaag
     9481 cttaacatga ataaaaaaga caagaacctc agttataagg atcttctata tgtcatcgta
     9541 gtagttgctg tcggtacttt tatgatttta aaagttccag agttgtttag atagttattc
     9601 tatcgaagat gatcatgaac tagaaaacac atctcctctt tgttagcagg tgtgtttttg
     9661 tgtggaagcc aagttgtttt ttcatctgct gtattccatg atacaattaa gaatcatgtc
     9721 tataaggaga taggaaataa atggcaaatg cgcttgaaat aaataatttg aaaaagatct
     9781 atggaagtgg ggtcgaagca ttgcggggaa tcgatctttc ggtgaaagaa ggagatttct
     9841 atgcgctatt agggccaaat ggagctggga agtctacaac gatcgggatt attacttctt
     9901 tagtcaataa aacatctgga aaagtgaatg tcttcggtta tgatctggac aaggatctag
     9961 ttcgagcaaa gcaacagatt ggattggttc cacaagagtt caattttaac ccttttgaaa
    10021 cggtccagca aatcgtcgtg aatcaagcag gctactacgg tgtctctcgc aaagaagcga
    10081 tgaaacgcag cgaaaaatat ttgaagcagt ccaatctttg ggaaaagaga aatgtccgtg
    10141 cacgtatgct ttccggggga atgaagcgaa gactgatgat tgctagggca ttgatgcatg
    10201 aaccacgttt gctgatcttg gatgaaccaa cagcaggagt tgacatcgag ttgagacgcg
    10261 agatgtggga gtttctacga gaattgaacg aaaatggtac aacgatcatt ttgacgacgc
    10321 attatctaga agaagctgaa atgctttgtc gcaacatcgg aatcatccaa tcgggtgaac
    10381 tgatcgagaa tacaagtatg aaagagcttc tgtcaaaact gcaatttgaa acatttatct
    10441 ttgatttagc gccatatgat accaaacctg tgattgaagg atatgcttgt acatttgaag
    10501 atgagcagac aatcgctgtc gaagttgaac gaaatcaagg ggtgaacgga atcttcgatc
    10561 agcttacaag acaaggaatc aaagttcttt ctatgaggaa taagtcgaat cgtttagaag
    10621 aactgttctt gaaaatcaca gaagaaaata atcatacgga ggagaaaaat gtttaatctt
    10681 tattttactg cattaaaaag tttagctatc aaggaaacga accgctattt gcgtatatgg
    10741 gtccaaacac tggttccgcc agtgatcacg acatccttgt attttgttat tttcggaaaa
    10801 atgatcggtg ggcggatcgg tgacatgggt ggtttttcat atatggaatt catcgttcct
    10861 ggtttgatta tgatgtctgc tatcactagt tcgtatgcca atgtttcttc gtccttcttc
    10921 tctcaaaaat ttcagaaaaa tatcgaagaa atcttggtag cacctgttcc tacgcatgtg
    10981 attatttggg gctttgtgat tggtggtgtg ggtcgcagta ttctcgtcgg ctctctcgta
    11041 acgatcattt ccttgttctt tgtaccactt catgtgtatt catggttcat ggtcatcatt
    11101 acattattga tgacggcaat cttgttttca ttagcaggac tgctcaacgg gatttttgct
    11161 cagtcttatg atgatgtatc gatcgtgcct acatttgtct tgcagccgtt aacttatcta
    11221 ggtggcgttt tctatgccat ctctatgtta ccgccctttt ggcaagctgt ttcaaaagtc
    11281 aatccgatcg tttatatgat ctccggtttc cgttacggat tccttggaac gatcgatgtg
    11341 ccagtagcaa catcgatgat cgtacttgtg ttattcatcg ttgttttgta cgccgttact
    11401 tggtatttga tcaataaagg aagaggattg agaagttaga aaagaggttg tgaaatcagc
    11461 gttttgacct gagtattaga gaaacaaacg ggaaaatagt tcctcatatt ttgttgtttt
    11521 ttgagcgaat accgagggtc agctgattga acaccgttta gtg
//
LOCUS       DASJST010000093        11393 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJST010000093 DASJST010000000
VERSION     DASJST010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.094"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1031)
                     /locus_tag="U4B05_001988"
     CDS             complement(<1..1031)
                     /locus_tag="U4B05_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2051115.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="U4B05_001989"
     CDS             complement(1113..1802)
                     /locus_tag="U4B05_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2051116.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="U4B05_001990"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="U4B05_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN2051117.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="U4B05_001991"
     CDS             complement(2329..2832)
                     /locus_tag="U4B05_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN2051118.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="U4B05_001992"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="U4B05_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN2051119.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="U4B05_001993"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="U4B05_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN2051120.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="U4B05_001994"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="U4B05_001994"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN2051121.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="U4B05_001995"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="U4B05_001995"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN2051122.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="U4B05_001996"
     CDS             complement(6444..7463)
                     /locus_tag="U4B05_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2051123.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="U4B05_001997"
     CDS             complement(7636..8541)
                     /locus_tag="U4B05_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2051124.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="U4B05_001998"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="U4B05_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN2051125.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="U4B05_001999"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="U4B05_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN2051126.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="U4B05_002000"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="U4B05_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2051127.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="U4B05_002001"
     CDS             9754..10398
                     /locus_tag="U4B05_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2051128.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="U4B05_002002"
     CDS             complement(10454..10957)
                     /locus_tag="U4B05_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2051129.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3315 a         2369 c         2084 g         3625 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcc
//
LOCUS       DASJST010000094        10964 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJST010000094 DASJST010000000
VERSION     DASJST010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.095"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..421
                     /locus_tag="U4B05_002003"
     CDS             <1..421
                     /locus_tag="U4B05_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051130.1"
                     /translation="VILVGCSNEKNKQTNQDNGVLKSGTMWKEEVGGLVYNLKIIDET
                     TWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFINKSDSLFIVVPYEKNG
                     VKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            418..660
                     /locus_tag="U4B05_002004"
     CDS             418..660
                     /locus_tag="U4B05_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN2051131.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            678..980
                     /locus_tag="U4B05_002005"
     CDS             678..980
                     /locus_tag="U4B05_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051132.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            1050..3113
                     /locus_tag="U4B05_002006"
     CDS             1050..3113
                     /locus_tag="U4B05_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051133.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            3113..5725
                     /locus_tag="U4B05_002007"
     CDS             3113..5725
                     /locus_tag="U4B05_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN2051134.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            5722..8253
                     /locus_tag="U4B05_002008"
     CDS             5722..8253
                     /locus_tag="U4B05_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051135.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            8303..8635
                     /locus_tag="U4B05_002009"
     CDS             8303..8635
                     /locus_tag="U4B05_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051136.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            8636..9229
                     /locus_tag="U4B05_002010"
     CDS             8636..9229
                     /locus_tag="U4B05_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051137.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            9392..>10964
                     /locus_tag="U4B05_002011"
     CDS             9392..>10964
                     /locus_tag="U4B05_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN2051138.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         4057 a         1721 c         2259 g         2927 t
ORIGIN      
        1 tgttatttta gtcggatgta gtaatgaaaa aaataaacaa actaatcaag ataatggcgt
       61 attgaaatca ggaactatgt ggaaagaaga agtaggtggt cttgtttata atttgaaaat
      121 tatagacgag actacctggg aatactctga gagtgtatgg catccagatc ctgtacaaat
      181 aacagtcaag aggcaaaaag actataaagg tttagaaaga tacaaaatag tagattcagc
      241 tggtgttagg gaatttataa acaaatctga ttctttattt attgttgttc cctatgagaa
      301 gaatggcgta aaaaaaataa tttttcttgg atctagcaag gatgaaaagc gaacgaaaga
      361 gaaattgatt cacgatggat cacaatccaa taaatataag ttacaaaaga ctagcgagtg
      421 attgctagtc tttttatttg tcctgaagga ggaatcacag tggcaaaagc taaaacgcca
      481 tttgaaaaag ccgcgtctaa aatcttagaa cgccgcggtg tatctgaaac acagtggaaa
      541 caagaagtcc ttgaaaaagc acaatttgac ctattacggg gaaaagacaa ggacttagaa
      601 ggatatatct atcaacgtga acgtgaaaag cttgttttcg atgaaattat taaacactaa
      661 aaggagcgaa caattttatg tttgaatcaa ttacaatgaa ttttttaaca caaccgaaag
      721 tagtagctgc ggttgatatt tttagagcat tacaaactgc cgcaactagt ttaaaaaaca
      781 atctgatgat ttttgctgct gctgcggccg ttgcttccat tgttattggg ggtcttttct
      841 tcctatttgg acgaagagga gctgaaaatg ggaagatgca cgtaggctat gtattgatgg
      901 gggtaggagt tattttagca gcagcttcta ttgtggtttc gttatttgga ttgtttggac
      961 aaacacctca aggattataa gaaagtaaaa aagtaggagt aagaagtagt cgatactttt
     1021 tactcctttt ttatgaaagg agaatactca tgtactttac gattgcaggc aataccaaaa
     1081 cagataaaga gttaataaaa aaagcaacga aggaagtagg aaagcgtact tcttttttac
     1141 cgaaaacaat taaatattct gtcattgaaa cggaagaaca gctacatgtg ttagggaaag
     1201 tactggaaca cgaaagtcga tcttctgtta agttgactct cacgttacat gacacagaag
     1261 gaaaaataga gaaagctggt acagaatacg tttttggccc actggattat caaatttcag
     1321 aaggtttcta tcctacattg atggatttaa tccgccttga tgtgtttaaa gaaacggatg
     1381 aaactatgag tgtggaagag tcttatcgaa acgaaaaact tttacaagtt ttagaagaag
     1441 agttagagag cgaccaaaga aaagtgaatc aagggagtga agtcgaagta aatgaagaag
     1501 taccatggga acgaccaata tttccaaaga aggagtcaaa agaaaaagag acagtctatg
     1561 ttgacgaaca agctgatatt ccgactcaga aatcgatatt tgaggaagag aacgatgtga
     1621 tgggtcaatt ggaggtagat ccctttgaag aaacaatgga ggatgaagag acggccgaag
     1681 tagaagttga tccagttgaa gcgaatgaag agtcaacatg gactgaaggg gattctcagg
     1741 aatatattga accacttatc agtgaagaag tagaaaaaaa cgaaatagag atacaaaagg
     1801 caactgtatt tgattatatt ttggatcaat atccgccaga gcatgaatgg atgaaagaaa
     1861 aaaacaaatt atttttaaat gagctttatt cgaaatatca attaccccaa acctatcaag
     1921 catttttaga gagtaaagaa gaattaatct cacagggtaa aggcacactt ttagaacagt
     1981 tagagtctgt ccaatcaaca aattggcatg agcttgcaca agaagattta ttagccacct
     2041 ttgaagaacg tcaacaagaa acagatcaag aaattaatga gtatgcgacg gaacagtcgt
     2101 ctaattggga gaaagcaaaa gaggaactaa ataaagaaga acagcgagtg attgaagaag
     2161 aagtacaaaa aattaaagag cgatacgaaa agaaacgcga caatgcgtat tccttgtgtg
     2221 tagcaaagat caataatttt actcagacga atcaagaagc gttatcgaaa gaaaaagaaa
     2281 acttgttaaa agaacgtatt aaagaacgaa aagaagaatt ttaccgaggt ttgcgtacag
     2341 ataagctaaa gattagcaaa gaattaaatg aacaactgaa taagctatat caaacaacga
     2401 ctgaagttct tatggaaaaa tacgaaaaga tccagcaaga attacaagtg caaattccaa
     2461 aatggaaaaa agagtatgaa attcaacaac aagaattgaa agaacaagaa gaaaaacaac
     2521 ggcaaaaaga aaaagagcgc gaacaattag aactaaaaaa acgtgaacag gatcttgcgg
     2581 aacaacaaat ggcaatagaa aaacaacggt taaaccaaga aaaggaaaaa gaagcagctg
     2641 ctcgtaagga acgtcaagaa caaatggata cattgcgaca agctcagtta aattatctag
     2701 cacaacctca accaacgcct tcagtagctg ctgttcaaac cccaatgaac aaccaaacaa
     2761 atctaactcc ttcaccacag cgttccttta acggatggat ggtgggatgt attgcaagtt
     2821 taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat caatcttcac
     2881 aggctgattc tgtggccaca acgacgtcaa tcaaggaaga agcccaagaa caagcaagaa
     2941 aagagtatga acaacaatta gaagaattat tgaatcgtac caatcagtca acaacagagt
     3001 caagcatgga aaatagcaaa gagagtgatt ctagtaaggc taaagaagga tcaagcgatt
     3061 caactgtaca atcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa taatgcgtgt
     3121 tagagaaaca acgaagcgaa aagacaaacg attcaattta gaaaagaata ggtatctttc
     3181 tcatttctat cgctatactc cctcaggaga agagctacca gaaaaacatg acaaacgcat
     3241 ttcgaaaaag attttttata gtggacttgt atacctccct ttagagtatt tagtaacaag
     3301 agtaccagaa gttttcaacc gattggaaat tgttaaggga agcttacaag gaataaatct
     3361 gtttcaatta caggaaatgc cacaggtctt tcattttgat tcagctggtt tttggaatcc
     3421 atttactcct acaactagtt tgggtacacg tttagcaatt tcgacagttt gtgtggttcc
     3481 agctgtactt atgggattga aaatggacat gtggtttcgt cctctagcag atggacaaaa
     3541 aggggataat cgattaatga ccttaaaaga aatccaacag atttatccgc aaatacctga
     3601 aacaaaaact acttttccag gttatggagg tattccaatc acacattaca aaaaatattg
     3661 gtacattcaa acagatactg ttaatacgtg tattgttggt acctctcgat cagggaaagg
     3721 tcagattgaa gtgttagcca ccattgacaa tttatctcgt gcagaaaaac aaagttcaat
     3781 ggtcgttaat gatccaaaaa cagaattata tgttggtagt aaagatgaac tagaagctag
     3841 agggtatgat gtatatgcct ttaatatcac cgatccactg caatcaatga gtgataatcc
     3901 attggcactc atcgtgaaat actggaaacg aggagacata gatactgcaa cacagttaac
     3961 caatactttc acaaatacga tttattatga tgccaacgct tcagataaca aatactttaa
     4021 cgaaaacgcg cagaaagctg tcaatgcgct tatttttgcg ttgttagaac aagctgatca
     4081 aacaggagat tactccaaat tgacaccgaa taatctcgtt gagcttcttt ctgagattgg
     4141 cggttttaat tattatccag atccagacaa tgaattcaaa cagttgaatg ccttagatga
     4201 atttatgaat cagttgccac ctggaaacat tgcaaaaaaa caatatgggg caacgaaaat
     4261 tggatctgat aaagcgaagg gaaatatttt atcaacggca atcacgggat tgaatccatt
     4321 tactctaacg aaaattgcaa agatgacttc tcaatcaacg attgattata aatcggttgg
     4381 gtttccaaaa tacttagatt tgaaattaca tgaaagtctt ttaaatcaac gagtcgtagt
     4441 agaattttat cataagggaa agaaatttca tgaagaacgt gtcaaagtag gctataaagg
     4501 attttgtgaa atcaattttg attgtcattt agttgcaggt gatcaagttt gtttaaaata
     4561 cgatcatcaa ggaaaagaat atgtggcttg gtatgaattt tctcaaagag tattgaaaga
     4621 cgaagagggt aatatcgttt atcggaaaaa acgaggagaa acccatttgc cagaattaga
     4681 aaaagaagcc gttctaacat tagatgagga gaaaagtaac ttattagtag agtctattcg
     4741 gatgcactat accgataaac caatggctat ttttatgctt acacctgatt atgatccatc
     4801 taaccatatc attgttagta tttttcttag tcagttatat aaagaattat ctacgcaatg
     4861 tgttaagact aaaggagata aatgtcaccg caggattcat tttattttag atgaatttgg
     4921 gaatatgcca ccaatggata atatggaagg aatcatgacg gtcacagctg gccgaaacat
     4981 gttgtgggat ctgtttattc aatcttatca gcaactttat gcgaagtatg gagaacaaaa
     5041 cgcagcgatt atcaaagaaa attgtcagac acatatttat attatgtcga cagatgataa
     5101 taccatcgaa gaattttcta aaaaagtggg gaataagacg gttgaacaag agaacagtac
     5161 catcaattca ttaggtatga atactcatat caatcgcaat gttgattcag atcgtatctt
     5221 aactcctgaa agaatttcta cttttttaga aggtgaaacg attgtactcg ctcctttaaa
     5281 acgacaagat ttgaaaggtc ataaaatacg gccgtatcct atttttaatc atggggaaac
     5341 gaatcttcct tatgcttatc aatatattga agatttcaat ccatcgtggg atatcaatga
     5401 tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa ttgattatga
     5461 ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg atcaattaga
     5521 agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tctcttgaga atgagacggc
     5581 taaaaaattg ttaattcaat tttacgaaga acaagcagca gatcgacttc aagaatatgt
     5641 tgaaaagttg atcaaaaata aagaaatttc tgaaatgatt gggaaacggt tgttattgga
     5701 atctgaaaag gtggtgaaag aatgatcatt ggaggaattt tttctggcat agccgactgg
     5761 tggtcaggct tattcggaga aggggatgca aaccccaaaa ccgtaacgaa agtcttacaa
     5821 gattatggta attatctgca gtatcaagat gtatttagtt ggatttggaa cacttttctg
     5881 tggggattga ttaaaggatt atataatctc aatactttac tagaaaaaac catttatcaa
     5941 agctttgatt taaaagactt attgaacgcc gctggggtaa atgcactatt tcaaagtttg
     6001 attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg cttgaaattt
     6061 tttatgagta aacacccacc aaaagtcaaa aatatcttgg ttaatttatt tatggccatg
     6121 atcttgattt ttggtgggag ttctttaatc gatgaagggt taaatatcag taaaagcttt
     6181 tacggagacg tcactgcagc aaataaagca gataaaacgg gttcacctgc ttttcaaatt
     6241 attcaaaata atgtgatcga tgtcagtaca ttactagcaa ctgacccgaa taaagtagaa
     6301 tccctgccaa cagacaaaag aaatgcttta acagcgaaaa attttaaaac agcgaatatc
     6361 aatgggatta ttactcctaa acaagctaaa gaaatggcgg ataaagataa gagtggttta
     6421 tcagatttac aaaaggatcg gttaagagcg ttacaatacc gattggaact agatgataat
     6481 gggaaagaaa ttcctgttca aattagtgac gaaggcttag caaaatatgt ctatacttca
     6541 gggtatcgtc gatacagtag taaaccaggt attatcttag ctggtgaatc ttctttggcg
     6601 gtggcctatt tatttatcct atttacgatc gttacttgca ttattgagct ggtatttaaa
     6661 aaattctatt tagtgattgc cgcgtcaacg gactttgaga caggccaacg gatgaaaagt
     6721 gcgatagaag atatttctca atccttcttg ttattggcgt ttacgatttt agaattacga
     6781 atctatacat taatgctttc aggtatcggt gatttgcatg cggcaggaaa gatcaatgga
     6841 ttcttgtatg ttgttgcttt aattgtgcta actattgcgc tattcaaagg ctctcaatcg
     6901 gttacgaaga ttttcggtgt tgatacgagt ctgaaaaacg gcagtaattc tcttcttagc
     6961 atgtttgcct taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa ccttgcaagt
     7021 gctggtaaga gtggtttaag ccatttaaat gatattcgta aaaatggatt tggccatcga
     7081 aaagatgtta cccctgataa cggggaagaa caatcagacg ataaagctac aactgtacct
     7141 aatagtaaga aaacagtttc cgaaatgttc gggaaagcga aaaatggtgt ttctaaagcc
     7201 gcagaaggtc ttggttacgt caatgaacgt gggttaaaag gaacagctga agatcttaca
     7261 gaaaaagcca aagacacctt agaagagaaa ttagacggca cgagagctaa agaattggct
     7321 gaagctgtga agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc ttcagaagtc
     7381 aaagaaggac taaaagatgc gtatggtgaa ggacaagtta atgcagcagt aaaagctaat
     7441 acaacaaccg aaaaagaaaa tcaagaaggt atagtgattc cttcagtcaa agatcaccaa
     7501 gaagcgttgc actctaatga aaattcgatt gaagagaaaa attccataga agaaagaaat
     7561 tcagaagaag gaaaaaatgg acagttacca aaaactgatc cagaacaata tcgctataaa
     7621 cctaacagtg accttgaaaa tgagaagcga atttcagatt tatcaaaagt aaatcaagaa
     7681 gacaaagcag aaaatgaggg acagttacca aaaactgatc cagaacaata tcggtataga
     7741 tctgttaact ccgaaaatga aaaacgcatt tcagatttac caaaacgaaa tcaagaagta
     7801 caaacggatg aggattccat cgttctgcca aatgataatg gaatcgaagt tggaactgaa
     7861 aaatcaacga aaattggttt acctaaggca caaaatttac caatgaaaga tagtcgtttg
     7921 aatcaattca aggagaatga tcaaagcttt gttcaccaat caactgcagg tactgttcaa
     7981 atgaaaccaa gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta caatttatct
     8041 gaaagtggtg agacagggaa tgcgtccgtt caaaatgttg tgacaagaga agctgcacca
     8101 aaacctattc aaaacgttca tacaggttac aagccagcaa atgtggcatt tactgcacca
     8161 acaaaaataa atgtgacaag agaaactaaa aacaggaaca atgcattgaa agaacaacta
     8221 ttgagggata tcaaacagga tatcaaacca taaaaggcct acaagttagg ccttttattt
     8281 atatgaaaag gagaaatcaa ttatggataa atatataaac atgaaaaacc ctccaagatt
     8341 tagtggagaa tggaagtttt tcgctttttt ctttttagat ctattaacgc ttggtggctg
     8401 ctttttttgg gcgaaaatgg tcaataatct tgtccactta ccactctttt ttgcactctt
     8461 gaattatggc ttatggattg cgatagcgtt agtgattgtt tggcgaccct ataataaccc
     8521 aggtcaacga caaatttttg cgattatcga gtctattttt tttgcggaca agaaccatta
     8581 tcatgcgtta gatcctaacc gaaattatca tggtacgtat aaggaggatg aataaatgcg
     8641 agtcaaagga caaaattcag ttgaaaggaa atccattttg cagtttggaa aaccagaaga
     8701 gcgctctatc ttggacttat ttccgtataa atcctgtaca gaagagggag tcttaattac
     8761 tagagaagat cattttcaac gttattatcg tgtagtttct acagacgtgg aaggattaaa
     8821 tgaacaagag aaactagaac gaatgaatca gttgaccacc gtcatgcgta cgtatgtgcc
     8881 aacaattaag ttagttagtt tgaccacaga gacggatcta acggaacaaa tcgtgcaaaa
     8941 gcggcaatta ttagataaaa atcgtatgga acagtcctta ggaagaaatc tacaacgatt
     9001 aaaaaaatat gaaaagaaat tagtcgaaga aatccaggaa ttgaaacgag cagaacaaga
     9061 acgtcctgac ttaagcttct ttttcgtcat tgaaggaaag acaatcaagg atttacaaac
     9121 cagaaaccgt cagttaatgc gatctagtgg cattttaggg ttaaaaccac tctacaaaaa
     9181 agaattgatc aagattctgt atcgaatgaa caatatgaat gatgagtagg aatggagaag
     9241 agagaatgaa atatccgttg atatggttag aaaaagatgt tccgaaggaa gagaccacta
     9301 gaaaatttcc tcacacttta agcctgggta agttgggaaa agggcaatct tttaaccaag
     9361 taaagaatca aacaagaaga ggtgactgta catgttagaa gcaattaaaa cagctttttc
     9421 accacaaaat gatcaaaaag tacttgatcc attaaagcaa ctaggaaatc gggaaataga
     9481 aattttaaaa tccaaagggt atgatttaga ttttttaaaa caagtgatgc cacaaggtgg
     9541 gttaaatttt gatgaagaat atgtgacgta tggtaatggt tattcacgat gtgtgacagt
     9601 ttatcactac acaaaaaatc ctacgctttt ttggttggcc aatctcatga acaatcctca
     9661 tacagtggcc acactcgatg ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa
     9721 tcgtgcctta gatgaattaa atgatcgatc agataatgaa cgaaaagcga ctgaccgaaa
     9781 taaaagcgca gaagaatacc tggaactagc ggaatatgct cgtaaattga cttctaatgg
     9841 ggaaatttct aagcaagtca aagtgagagt acatgtttac ggaaacacga ttcaagaagt
     9901 agatgaacga gcgcaggaac tgatcgaaaa tctaaaaggg atggattatc gtgcggtgac
     9961 ttatctgcat aaaaccaaac gagattggca atcgatgttt accatgttcg atgaacaagc
    10021 tgaatggttc ggaaatatca aagggcaatc actaccatcg ttaaacattg gcggaggttt
    10081 cccgttccac caccaaacgc taaaagatcc aggagggaca caatatgggg aaaccatgac
    10141 aggcggccca tttgtctgga atgcgacgta taaaaatagt gtacggttgt ctactaatac
    10201 cttgcttcta ggaatgatgg gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc
    10261 ccatttagct gcaggagact ttatttgggg ctttgaaaag ggaaaagact ttattccttt
    10321 cttaaaggaa tataacggta ttatggtgcg tttagacgga tctgacggaa tgatcaatcc
    10381 tttagagata tttgccacac ggacgtatga tgaagcatcc agtttatatg acgatggatc
    10441 agtcaaagat ttgaagatca atgaagcagc gtcctatcaa acacacttag acaaagtggt
    10501 ctatcaggta cagttagtga gtcctcagct aaaaggaacg atgaaagcag aatttaagac
    10561 ctacttaaat cgtttttatg aagagtatgg aacagtacca cgcggcttta cttcctcaaa
    10621 ttcgcgttca aatacggaga cgcaagtcac aggtaaagat ccagaagctt atccgacgtt
    10681 caaagaattt ttagactttt taggacaact ggaattacca ggtgcttccc aagagaaaaa
    10741 gaatcgaaaa gaagaaatgg aatccatcgt agaatcctta tgtgagacgt atggcatgat
    10801 ttttgatggg catagtacga ttcgacacct tgaccaacaa cagttggtct tttttgacat
    10861 tgactctatt acaggcttag atcgaggggt acaacaatgt caaatgtaca tggctatgac
    10921 gttgatttgg aatcaggcat tgattcaagg aagaagacaa cgac
//
LOCUS       DASJST010000095        10908 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJST010000095 DASJST010000000
VERSION     DASJST010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.096"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..736
                     /locus_tag="U4B05_002012"
     CDS             8..736
                     /locus_tag="U4B05_002012"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN2051139.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            736..990
                     /gene="purS"
                     /locus_tag="U4B05_002013"
     CDS             736..990
                     /gene="purS"
                     /locus_tag="U4B05_002013"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN2051140.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            992..1663
                     /gene="purQ"
                     /locus_tag="U4B05_002014"
     CDS             992..1663
                     /gene="purQ"
                     /locus_tag="U4B05_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN2051141.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1664..3886
                     /gene="purL"
                     /locus_tag="U4B05_002015"
     CDS             1664..3886
                     /gene="purL"
                     /locus_tag="U4B05_002015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN2051142.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3871..5310
                     /locus_tag="U4B05_002016"
     CDS             3871..5310
                     /locus_tag="U4B05_002016"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN2051143.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5333..6385
                     /locus_tag="U4B05_002017"
     CDS             5333..6385
                     /locus_tag="U4B05_002017"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN2051144.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6382..6960
                     /gene="purN"
                     /locus_tag="U4B05_002018"
     CDS             6382..6960
                     /gene="purN"
                     /locus_tag="U4B05_002018"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN2051145.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6957..8492
                     /gene="purH"
                     /locus_tag="U4B05_002019"
     CDS             6957..8492
                     /gene="purH"
                     /locus_tag="U4B05_002019"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN2051146.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8508..9782
                     /gene="purD"
                     /locus_tag="U4B05_002020"
     CDS             8508..9782
                     /gene="purD"
                     /locus_tag="U4B05_002020"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN2051147.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9904..10818
                     /locus_tag="U4B05_002021"
     CDS             9904..10818
                     /locus_tag="U4B05_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2051148.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT         3582 a         1853 c         2604 g         2869 t
ORIGIN      
        1 gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac
       61 ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt tgaatggcgc
      121 gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa gtgcaatttt
      181 ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt ctaaaaccga
      241 acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa atattgcagc
      301 aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca aaccaatcat
      361 tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag agcatatcta
      421 tttcttacaa cttgcaaagc cggaagaaat cagtgagctg aaagcccaag cgttggctat
      481 taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag atttcaagct
      541 agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac cggatacttg
      601 ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc gcagagatct
      661 aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac taactgaaga
      721 aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta
      781 gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga aacaatcgaa
      841 gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga accaatcgat
      901 acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga aacataccga
      961 tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg tttttccagg
     1021 ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg ctgatgccga
     1081 atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac tgccgggagg
     1141 attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta caatcatgga
     1201 agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca atggattcca
     1261 gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt ctttgcattt
     1321 tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt cagagtatga
     1381 agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt tctgtgacga
     1441 agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata atgacaacat
     1501 caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg tgttaggaat
     1561 gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg ggaaacggtt
     1621 ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga
     1681 accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat tgactgatga
     1741 agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata cggaaactgg
     1801 actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac cggttctacg
     1861 caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt
     1921 cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc atccttcagc
     1981 cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag atattttcag
     2041 catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat tagataatcc
     2101 aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg gcaattgtat
     2161 cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag gaaacccatt
     2221 agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag ggcaagccgc
     2281 tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg
     2341 agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt ctgctgtaca
     2401 agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat taatcttgca
     2461 tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg tttcatctag
     2521 tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg aagtcccaca
     2581 aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag aacggatgtt
     2641 gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga agtatgatct
     2701 agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat atcatcaagg
     2761 agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag tttatcataa
     2821 agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt atcagccagt
     2881 tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga ttgcttcaaa
     2941 acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg tccgtccagg
     3001 aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta tgaccacaga
     3061 ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag cagtggcaga
     3121 agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg actgcctgaa
     3181 ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag ctgacggaat
     3241 tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct ctttatataa
     3301 tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg gattgattga
     3361 ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa tctatatatt
     3421 aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc ttggaagaat
     3481 cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag agttagtatt
     3541 gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag gtggcttggc
     3601 aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac agtttgaatt
     3661 gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag tcgcaccgga
     3721 aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag gtaaggtgac
     3781 ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag aaactgcagt
     3841 agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa
     3901 gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc atacttcggg
     3961 ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga
     4021 aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga tcaaagaaaa
     4081 ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac agcaggcaat
     4141 ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc gatcggctta
     4201 gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga ggaaaatggg
     4261 gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg acgaagtgaa
     4321 gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg tggctttgcc
     4381 tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca
     4441 ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac ttgtgcatta
     4501 gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt gatcatcaat
     4561 gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat ttgttcgatg
     4621 gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt gcatactgca
     4681 cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt
     4741 ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag tggaatccct
     4801 tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca gccaacgcct
     4861 gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt
     4921 aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag acggatcgtc
     4981 catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg
     5041 aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat tgcggcaaat
     5101 cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa
     5161 gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg attgtgtatg
     5221 gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa atatcaggaa
     5281 tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt atatgaattc
     5341 aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg aagtagtaga
     5401 acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg ctataggagg
     5461 attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg
     5521 gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac acgatacaat
     5581 cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt
     5641 atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg aaaatgtagt
     5701 aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg gagaaacagc
     5761 tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg ctgtagggat
     5821 agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg tgttgcttgg
     5881 aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa ttttctttga
     5941 gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac ttggcgaaga
     6001 attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca aagaaaagaa
     6061 aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt
     6121 accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc caatattcgg
     6181 ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct tcaatatggg
     6241 catcggtatg gtcctggcag tccaagaatc gaaagtaaaa gaaatagtac acgaaattga
     6301 gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt
     6361 ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa cggaagcaac
     6421 tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat tgactggttg
     6481 ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca
     6541 gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc aattttgcat
     6601 aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg aatcattgga
     6661 ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc tcttttacct
     6721 gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa agaaacgggt
     6781 gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg gcaggaaaaa
     6841 gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca tagagtagag
     6901 catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta tctgaaatga
     6961 caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc caaggtttga
     7021 ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg gataaagctg
     7081 ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg gatggccgtg
     7141 tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat ctccctagcc
     7201 atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat
     7261 atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct attgaaaaca
     7321 ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct
     7381 ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag tatgggcata
     7441 cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact gcggcatatg
     7501 atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg gaaaaattga
     7561 cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat caagccgctg
     7621 ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa caactccatg
     7681 ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg atcatgaagg
     7741 aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt attgggacag
     7801 catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt tcaatctttg
     7861 gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag atgcataagc
     7921 tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa ttactgcaaa
     7981 aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat caaaatcgag
     8041 aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa
     8101 atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat
     8161 tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg
     8221 aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca gtaaaaattg
     8281 cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat gcttattttc
     8341 caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc attcagccag
     8401 gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat ctagcaatgg
     8461 tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg aacattttgg
     8521 tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc agtccgaagg
     8581 tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt atcaattgtg
     8641 tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa gaggctattg
     8701 attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat gattttatgg
     8761 cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa
     8821 gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc agaagttttg
     8881 aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag
     8941 cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg
     9001 atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg
     9061 tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt aataccaagg
     9121 tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat aaagggccaa
     9181 atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag atgattgaaa
     9241 aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta ggacgctctt
     9301 tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat
     9361 tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg aagagtgact
     9421 ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag tggcagcaag
     9481 aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa tatgagacag
     9541 gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct ggagtttcct
     9601 caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa gcatacggtg
     9661 agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa acgacagatg
     9721 gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg atcgtgaagt
     9781 aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg ccgattttag
     9841 tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata aggaggagtt
     9901 cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt gatcttaggc
     9961 tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac
    10021 ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga agaaatcttt
    10081 gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca aacaaaatgt
    10141 ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga atctgtagaa
    10201 aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca tcgtccagat
    10261 acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa
    10321 gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat
    10381 gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac tggaatgatc
    10441 gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga cggtagtgta
    10501 cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata ccagtatgga
    10561 ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca aacattagaa
    10621 gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg gattttacgc
    10681 catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat caaggaaatc
    10741 gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta ccgtgctgct
    10801 ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa attaaaaaga
    10861 cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttt
//
LOCUS       DASJST010000096        10765 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJST010000096 DASJST010000000
VERSION     DASJST010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.097"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..578
                     /locus_tag="U4B05_002022"
     CDS             <1..578
                     /locus_tag="U4B05_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2051149.1"
                     /translation="PIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDFS
                     LVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVADY
                     LLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPLL
                     FEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            578..1507
                     /locus_tag="U4B05_002023"
     CDS             578..1507
                     /locus_tag="U4B05_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051150.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1504..3240
                     /locus_tag="U4B05_002024"
     CDS             1504..3240
                     /locus_tag="U4B05_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2051151.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3303..4139)
                     /gene="pheA"
                     /locus_tag="U4B05_002025"
     CDS             complement(3303..4139)
                     /gene="pheA"
                     /locus_tag="U4B05_002025"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN2051152.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4136..4645)
                     /locus_tag="U4B05_002026"
     CDS             complement(4136..4645)
                     /locus_tag="U4B05_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN2051153.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4638..5933)
                     /gene="aroA"
                     /locus_tag="U4B05_002027"
     CDS             complement(4638..5933)
                     /gene="aroA"
                     /locus_tag="U4B05_002027"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN2051154.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(5946..7037)
                     /locus_tag="U4B05_002028"
     CDS             complement(5946..7037)
                     /locus_tag="U4B05_002028"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN2051155.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7054..8220)
                     /gene="aroC"
                     /locus_tag="U4B05_002029"
     CDS             complement(7054..8220)
                     /gene="aroC"
                     /locus_tag="U4B05_002029"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN2051156.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8222..9286)
                     /gene="aroB"
                     /locus_tag="U4B05_002030"
     CDS             complement(8222..9286)
                     /gene="aroB"
                     /locus_tag="U4B05_002030"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN2051157.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9319..10341)
                     /gene="aroF"
                     /locus_tag="U4B05_002031"
     CDS             complement(9319..10341)
                     /gene="aroF"
                     /locus_tag="U4B05_002031"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN2051158.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         2976 a         2384 c         1985 g         3420 t
ORIGIN      
        1 tgccaatacg cgtaattctt atcgatgatg aacagacgat tttagatgga atgaaacagt
       61 tgatcaattg gtctgagcac gggtttcaat taatagggac tttttccagt ccgctgaaag
      121 cgcttgattt ttctttggta catgcaatcg atatcgtggt cacagatgta atgatgcctg
      181 aattaaatgg aatcgaatta tcgcggctat taaaacagca acaacctgaa acacagatcc
      241 ttatcctctc tagctatgat gaatttgata tggttaaaga ttcttttaaa gaaggcgtcg
      301 ctgattatct tttaaagcct cgattgagtc ctgatttttt tctaaattcc ttaactgccg
      361 tttcacaaaa aatcaaaaaa aagcccaaca aaagcccaat caccaatcgt tatgagcaga
      421 tctgcgagaa tctttcacag cagattgctg gacaaactcg tccattgctt gaccctcttc
      481 tttttgaaca caccttgttt ttctgttata taccgaaaaa cggccattca gttcaaaaca
      541 aggctctaaa caaaaacagt cgatcaccca ttcattgatg gatagcgacg attcgtttac
      601 tatttatcct gtttctacct tttcggaaga atccggctat gtaatcaata ctgattccaa
      661 agatacactt aggaagtttg ttcaaaagtt tcgtcaagaa gtttttccag gtgatttttt
      721 tgttgtttct gattcttttg caatccaaga tatcaatgaa atattccaag agatcagaaa
      781 ttgcaccaaa ggacaacgtt tctatcataa gggaaagaac cttgcttatc agcatgaact
      841 tcttccgtta ttaaccaacc acgagcagca agcaagtagt tatttaacaa aaatcgttca
      901 taaagactac gctttagctg tcaaagaact tgagaatttc ttcacgacga tattggaaca
      961 gcctattgat ccagcttatt tgaaacatga ggcaatcaat cgtttctatg cattactcaa
     1021 cgctttggct gacgagcatg ttgggaatga agaactttct ttgctcaaga tcgcccttcc
     1081 ttctttatta gccgatgctg agtatttcga agatttttct attcttgtca aagaaacact
     1141 tgagaaactc acccactttt ctgcaaatgc aaaaaaagac aatttcgata tcttgacttt
     1201 gatctacgag ttcgtccagg aaaattatga gaaagaaatc aatttacaac ttttatccga
     1261 aaaatatcat ttttcatata gctatctctc agccatcttt actgaaaaat ttggtatcaa
     1321 cttttcaaaa tatctaaaaa aagtccgtat cagtaaagcc aagctctttt tgacggaaac
     1381 acggcttact ttgacggaaa tctgtgagaa agtcggctat accgaactgg gttatttctc
     1441 cagagttttt aaagaagaga caggaatgac accttctcaa taccgtaaag gaacttttgt
     1501 aaaatgagaa aaaaaatcaa agagcatgaa ctctttgcca aacttctgtt cattttgttc
     1561 atcggtcttt ttctgcaagc catcgtcatt tctttgttta tttatcatcg atcaagggat
     1621 gcctatatcc agctgttcaa tcaatcgaat gatgtggtac taaaaaagat tcaatctgaa
     1681 tttgaaagtt tgaatgatac aatcgagaat acattagccg cttttgattc taatccagct
     1741 gtcaaaagct atttttccaa tgatccagcc caacatatgg aacagcttca gcaactaaga
     1801 acaatccaaa aaatgaatga ctctctttca aaaatccatc caatgatcga ttacgatgta
     1861 ttgatctttg gagaaaatgg aagaactttt gtcggaaacg acatgctcac tgccgtatct
     1921 gccgattctt tttttcaatc tgcgattgct cagagagtga atgaacgtgc tgctgataca
     1981 caaatgcttt ttgcacacca cggattgact cttcgcgata agaaagcacc cagcgtcttc
     2041 tttgtcaaaa aactgaaaaa tacattgaat cacgtttacg gatatgctgt tttatcgatc
     2101 tcatccaagc aactagcgaa tctttttcaa tctgtagtaa atcctgagat atcgaagatc
     2161 tccttcatga acgaagaaca gacgatcatc gcatcaaatg aagaaaatac aattgggagc
     2221 cgatcaatga ttttcaaaaa actaacagta ggtgaaacaa cgatttcaaa tggacagcag
     2281 ttgacccgtc ttcctctgta ccgccaggat tgtacattac tcagtcaagt cgatcttcat
     2341 tctttatcta accaaatggg cgtgattttt ccgattattt tgtataatat cgtgacactt
     2401 gcttttgctg gctcattggt atttatgtat ttggatcgac acacaaaatc gatctaccgc
     2461 ttgattgatt cgctaaaacg gctagaggaa gatggcttaa aagctactgt tcctgtacaa
     2521 ggaagccatg aagtccaatt gctagggaat acgatcaatc aattgctaag taatgctcag
     2581 catcattatg aacagacgat ccaagacgaa aagaagaaac gttcgcttga aatccaaact
     2641 atgcaagcac agatacagcc ccattttatt tataatacgc tgactagctt gaaatttttg
     2701 atttggcaga aaaaaacaga tgaagcagtt caaggaatcg acagtttcat tactttacta
     2761 agaagtacga ttgggaaaaa agaagaagtg attccagtca aggaagaaat caaaagcgta
     2821 caaagttaca tcgatctttt gagtatgcga tatggcgaaa gaatctcggc taaaataatg
     2881 attcctgatg aactgcagtc attatctatg ccgaatatga tccttcaacc aattatcgaa
     2941 aacgcctatc ttcatgcttt tcacaataaa acgcaaggat tcatcacggt gtacggcaaa
     3001 acaaaaaatc agcttctttt attcgaagtg attgataacg gcgatggctt cgatactgaa
     3061 aataccaaac aaaaaaatga tcatttttca ggaatcggca tcagccatgt agatgagcgg
     3121 atacgcctaa tgtatggcga tgcatatggc ttaacgatcc aatcagtgat tgggacagga
     3181 acgactgtaa cgatttgtct accgcttctt cctaaaaatc aatcagaata tcaaaaataa
     3241 agaatagcca agcgctgatt tttttatttt aagttaaata aatcaatcag tattcttgac
     3301 tttcatctac gcgtctcttc tactgtcagc actggataac agcccaatat ttgactttct
     3361 cctccgagca tcttgatttc ttggagcgca ttctctacta atgtcattgg acggtccaac
     3421 aacaaatcga tcacgaaaaa atattctccc aagctagttt tcaatggtcg ggattcaatc
     3481 ttacttaagt tgatttcccg ccagccaaaa gctgccagca ttttatgaag catccctggc
     3541 cgattagcag gtagtgtcaa gatgacactc atttttactg gagctgactg ctggctggtc
     3601 atctttcggt caccgacgat ccaaaaacgt gtctggttca actcattatc ttggatattc
     3661 tcagctaaga tctccaatcc aacatgctgt gctgtttcta atgaagcaat cgctgctgcg
     3721 tcttctttcg gatgttctgc tacatacatt gctgcagcag ttgttgattc tgttgctact
     3781 aaaggaacat taggataatg cgtttctaaa aactgttgtg attgtgctag tgcttgtgga
     3841 tgtgaaagga tttttgtaat ctttgttgct ggattgccta aaagctgctg cttgatagga
     3901 agaacgattt cacttttaac ttcgacttct ggatgtttgg aaagaagatc gattgtgtga
     3961 tgaacagctc cttccaatga attctctaca ggaacgacag ccagatcgac ttgtttgcga
     4021 aacaaagcgc gcaaacaaac agggatggaa gcatacgcag tcaattgttc tgtaggaaat
     4081 agctgacaag ccgcctgata agtgaatgaa ctttctggcc ctagatagct gacgttcata
     4141 tgcttttgat cctttctata atggtatcga tgatcttttc tggtgtttga ttggtcgtgt
     4201 tgattcgatg tgtcgcactt tcttcatacc aagaaagtcg ttgtgccaat aatgctttta
     4261 tttcttctct tgtttttcca agagctaaag gtcgaataga cgtttcatct tgttggatac
     4321 gatcaactag acagtccgct tctgcctcta agtaaagaac aatcggctgt gtttttaaaa
     4381 aacgccgatt ttctgcactt tggacaatcc ctccaccagt cgcgataatt ttgtcttcat
     4441 ttgacagctt tcttaaaata tctgactcca cttttcgaaa gcttttttca ccgtaatagt
     4501 gaaaatattc ggcaatcgac aattggatat gttcctcaat ctttgtatct aaatctacat
     4561 actctttttg taaacattga gctaaccctt taccgattgt tgtttttcca gcgcccatga
     4621 aaccaatcag caaaatgcta gccatgagct tctcctcctg acacaagttc tgctaaatcg
     4681 tcaaaaaatg ccggataaga gattgaaaca gcttcagctc cttctagttc gcaaggtcca
     4741 tcagctatca aagcagctac ttgcagcatc ataccgattc gatgatcgcc tctgctggac
     4801 acttttgcgc catgcagagg tgtcggccca tggatgatca gaccgtctgc tgtcgctttt
     4861 atctttgctc ccattttctg taattcttct gccaccgcat caattctgtt ggtttctttt
     4921 acttttaatt cttctgcgtc tttgatgatc gtgattcctt ctgcttgagt agccactaaa
     4981 gctagaatgg gcaattcatc aatcagacgc ggaatgattt ctccaccaat cgttgctttc
     5041 ttcaaatgac tcgtcttgac acttaaatca gccgattggt tctgttcatt gtgattcgtc
     5101 tgggtaatcg ctgcgcccat ctcctccaac acatctaaga taccagttct tgttggattg
     5161 accccgacat tttttaacaa cagttggctt tccggaacaa gaagtcctgc tgctagaaag
     5221 aaagcagctg atgaaatatc acctggaaca gttatctgtt gaccagttaa tttttgcggt
     5281 ccggtcacta tgatcgtttt atcttccact gtgatccttc cgccaaattg acgaatcatt
     5341 tcttccgtat ggttacggga acgttctttt tcaacgatta cggatgtgcc ttctgcttgt
     5401 aatgccgcaa acagcaaagc agatttgact tgcgcactgg ctacaggcat attgtatcgg
     5461 ataggtgata aagagcgggt tcctgtcaca gtgattggta gatactggtc attttcttct
     5521 ccttgcaagt cagcgcccat ctcgcgtaaa ggttccatca ctcgccccat tggacgttta
     5581 gacaacgaag catccccgaa cagagtagtc gtaaaaggct gtccagccaa aatacccatc
     5641 aataaacgta tggtcgtacc ggagtttccc atgtcaattg attcggttgg ttggacaaat
     5701 gagtggctac cgcgaccatg aatcttgatc acctcttcct cttcttcgat ctcgacacct
     5761 aattgttgga aggcatgtaa agtactcaga caatcttctg cgcgcaaaaa attttgtata
     5821 gtcgtcgttc cttcagcaat tgctccaaac atgatgctgc gatgggaaat tgatttatct
     5881 gctggtatcc taacagtccc tcttaatcca tgtatttgtt gtaataattg catttatcca
     5941 gcctcctatt tgatccagca atgataactg gtattagaca taatgattct ttttgcttgt
     6001 aacaaatcct gttggttttt aaaactgatt tgcagtattc ctaaaatatc ttccctggtc
     6061 tcttggatct tcaaattaat caaagaaact cccgcttttc cgatcagtcc agttacttct
     6121 gcaataacgc caggttcatc tggcacatcg ataaataaat caaaaaaggc aggaatcgcg
     6181 ccttgcttat ggacaggcaa ctggtctcga ctttcttttg cccgattgaa aaaagaaaag
     6241 atttccgatt ggttctcagt catgatccat tctgctattt gtgtcatttc ttgttgccaa
     6301 tctgccaatc gttccaataa tatctgctta ttgctcaaca aaatatctgt ccacatgaca
     6361 ggatctgagg aagcgatcct agtgatatct cgaaagcctc ctgctgccag ttgtttggct
     6421 cgcggatact cttgattgaa ttgttctgcc tgattgacta agccagctgc cagaatatgt
     6481 ggaaaatgac ttagcattcc tgttatacgg tcatgttctc ttgctgtaag agttacatat
     6541 tttgctttgg ttccgcaaaa tagttcttgc agtttttcaa catctttttt cgtcttttcc
     6601 aaaggtgtaa agatgtaata ggcattttca aatagtttag catctgctgc ctgtatacct
     6661 gatttatgag aaccagccat tggatgtcca ccgacaaacc gaagattttt ctgctcagcg
     6721 gatgtcacga cttgctgctt cgtactgctg acatccgtaa tgatgacgtg atcagacaag
     6781 ttgattcttt caaggacatc aagataagct aaggttgtac ggacgggaac ggctaataag
     6841 ataacgtctg cttgttctgc acctgtttca aaatcacttg caacgtaatc aacgatctgc
     6901 tgttctactg cttgctcacg ggtttttgac tgactatccc aaccaactac tatcagttct
     6961 ggattggtct gacgaataca aagtgccata gaagcaccaa tcagtccaag ccccacgata
     7021 aaaacatgtt gtgccatctt atttgccccc tttttagtaa tgtttcaagt agtcacggta
     7081 ctccttcact gcagctttca actcaacaag cgaatcactt gggaattttg acaatagctc
     7141 tgtcgctacc acagtggcta caacattttc cgcaacgacg ctagctgcag gaactgctgt
     7201 actatccgaa cgttctacac ttgctttgta aggttctttt gtatcgatat caacactttg
     7261 aagtggcttg tacaatgtag gaatgggttt catgaccccg cgaatgacaa gtggttcgcc
     7321 attggtcatt ccgccttcga agccgcctag attattggta cgtcggaaat agcctctttc
     7381 ttcttcccat aggatctcat ccatcacttc tgagcctggt aatgaaccag aagtgaatcc
     7441 gaccccaaat tctacgcctt taaaggcatt gatactaacc acagcttgtg cgattttggc
     7501 atctagcttc gtgtcccatt gtacgtagct tcctaatcca gctggaacgc ctccaacgag
     7561 cacttctaca attcctccga tcgtatctcc atttttcttt gtctgatcga tcaaatcttt
     7621 tattctttgc tcaactgctg gatcaacaac tcgtacttct gacgcttcag aaatttcttt
     7681 gatttcttga atcgagatat ttccaggtat ttctgcttgg attccaccaa gacaaatcac
     7741 gtgtcctgct acgatgatgt caagttcttc cagaattttc ttggcaattg cgccgattgc
     7801 tacacgcatg gtcgtctctc ttgcagagga ccgttcaagc acattccgaa gatctcgatg
     7861 atggtatttc ataccgccta ccaaatccgc gtgtcctgga cgcggacgtg caactcgccg
     7921 cagtgattgg tctttttcag gcacttctgc tacagacatg acagttgtcc aatttttcca
     7981 atctttattt tctaccacta aagtgacagg tgaaccaatt gttttgccgt gacggatgcc
     8041 agacgtgatc cgaacacgat ctttttcgat cttcatcctt cctcctctgc cgtaaccagc
     8101 ttgtctgcga gcgagttcgt gattgatttc ttcttctgac agaggtaatc ctgctggtag
     8161 tccttcaata atggctgtta attctggtcc atgggattct cccgcagtca tataacgcat
     8221 cttacagtcc tcctaagtaa tctttgaatg tctctgtggg aacaggaacg atttttgctt
     8281 gcccaatctt ttctagcaag atgatattca attggcttcc tcttgctttc ttatcatggg
     8341 ttatcgcgtg gaataattca tctggatgac ttagatcgta cgtaatcggc agatggaatt
     8401 tttgaagtat cctttctaaa gcagctgtcg atccagttgg agacaagccg attttttctg
     8461 catgagaagt gatggctacc attccaatag ctactccttc tccatgactg actaccccat
     8521 atcctgctgt attttcaatt gcatggccaa ttgtatgacc aaaatttaaa ataagtcgat
     8581 ttccttggtc aaagacatct tcttccacta cttttcgttt gatttccaac gtagcagtaa
     8641 tgattggaac agcatgttcc agaagatctt tttcgtcttt cagctcgttc agctgtttcc
     8701 aaagcgttgc atctgctata gctgctgatt tgatgatttc agcgattcct tctcgtaaac
     8761 gacgtaccgg caaagttttt agaacctcag gatcaatcaa tacaccagct ggctggctaa
     8821 atgttccgac aaggtttttc gctttgtttg tattgactgc tgtcttcccg ccaatactag
     8881 aatctacttg tgcaagtagc gtagtcggaa tttgaacgaa atggatccca cgcatgaacg
     8941 tcgaggccac gaatgctgct agatcaccca ccattcctcc accaagtgca agtatcccat
     9001 cgctgcgggt aaaatcatta tctgctaaaa aatcgtataa ctcagctgct tgttctaaag
     9061 atttactggt ctcacccgcc ggtacgattg aaaggactgg ttcaaaatta gcttgtctca
     9121 attggaacat gattttttct gcatatagtg gagcaacgtt tgtatcagtg atcacagcta
     9181 ctttttgagg tctccaaatc gtttgaagcc atgatccagc ctgttctagt gcgccttttt
     9241 caatatgaat atcgtatttt tggttatcaa catgtacagt aagcattcgt ttcacttctt
     9301 tcttttaaaa agctggaatt aatcgagtgt ctgaatcttt tccattgctt ctttcatgat
     9361 cgcaacttct ttcatcatgc gtgagtaagt cttttcattc aacgattggg gaccgtctga
     9421 ccaagcattt gcaggatcag gatggatctc tacgatcatg ccatctgctc cgcttgccac
     9481 acccgcacga gccattggtg gtacaagttc ccaaattcct gtaccatgac ttggatcaac
     9541 aatcacaggg aaatgagtta attttttcat caatgggaca gcacttaaat caaacgtatt
     9601 acgtgtcgct gtttcgtaag tccgaatccc acgttcgata aaaatgacag gagatttgtc
     9661 ttcgactgca atgtattctg cagcattaag ccattcattg atcgtaccgg aaattccacg
     9721 tttcaatcca acaggtttac ctgttttacc gacagcagat agcagtttaa aattttgcat
     9781 attacgtgcg ccaatctgta ggatatcgct gtactcagcc accatatcga tatgcccttc
     9841 atccatcact tcagtaatca ctttcattcc aaattcatca gccgcttgac ggatgtattt
     9901 caatccttct tcttctaaac cttggaaagc atatggagaa gtacgtggtt tgaaggcacc
     9961 acctcgcaga atttttgcgc ccccggcttt ggccattcgc gcacattctc tgatttggtc
    10021 taatccttcg atagaacaag gaccagccat cgtcacgaaa ctgccgtcac caatttttac
    10081 tccgtctaca tccacgactg tattgccagg atggaattct cgagaagtca atttgtatgt
    10141 gtttaaaata cgcacagcat tttctactcc ttcatagcgt gtaaatgctc cttcttgtaa
    10201 acttcgcgta tcacctttta gtccaatcac tactcgttct gttccttgat cgattgagac
    10261 tttgaatcct tctttttcga tatgatcgac gacttttttt atttgttctt ccgttgcttc
    10321 ttttttcata attactatca ttgtttttcc cgctttcttt tgtttactta tctatcttgg
    10381 ctgattttct ctactttttc aaggtggttt tacattaatt cctactaaat gaccgtgtcc
    10441 tgttgttctc attttcgctc cttctttcct acttatgacc cagataatca acaaaaaaag
    10501 atgtccctgt aggaaattgc ctctacagtg aacatcttca acgagcctta aaaagcgctg
    10561 caaaaatata aaatagccac tatagatgaa tttctctacg tggctgtaag tcgtgtttaa
    10621 aaatctttag ccatcataga tacaaacctg gtttgtttgt acccacgaag tcatgaatac
    10681 aaacctatct aaaatagcta aagtaatacg tattcagttt tgtaagtttt ctagatttca
    10741 taaaaaatcg ctccaaactg atgta
//
LOCUS       DASJST010000097        10505 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJST010000097 DASJST010000000
VERSION     DASJST010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.098"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..2275
                     /gene="pcrA"
                     /locus_tag="U4B05_002032"
     CDS             41..2275
                     /gene="pcrA"
                     /locus_tag="U4B05_002032"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN2051159.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2292..4328
                     /gene="ligA"
                     /locus_tag="U4B05_002033"
     CDS             2292..4328
                     /gene="ligA"
                     /locus_tag="U4B05_002033"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN2051160.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4434..4739
                     /gene="gatC"
                     /locus_tag="U4B05_002034"
     CDS             4434..4739
                     /gene="gatC"
                     /locus_tag="U4B05_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN2051161.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4739..6211
                     /gene="gatA"
                     /locus_tag="U4B05_002035"
     CDS             4739..6211
                     /gene="gatA"
                     /locus_tag="U4B05_002035"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN2051162.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            6208..7638
                     /gene="gatB"
                     /locus_tag="U4B05_002036"
     CDS             6208..7638
                     /gene="gatB"
                     /locus_tag="U4B05_002036"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN2051163.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7648..8703
                     /locus_tag="U4B05_002037"
     CDS             7648..8703
                     /locus_tag="U4B05_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2051164.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            8814..10187
                     /gene="rlmD"
                     /locus_tag="U4B05_002038"
     CDS             8814..10187
                     /gene="rlmD"
                     /locus_tag="U4B05_002038"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2051165.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            10190..10318
                     /locus_tag="U4B05_002039"
     CDS             10190..10318
                     /locus_tag="U4B05_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2051166.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         3630 a         1890 c         2335 g         2650 t
ORIGIN      
        1 gctataatta acaagattga taaaagaaag gatgttgtta atgtcatcca aagctgaatt
       61 attaaacggg atgaaccccc gtcagaaaga agctgttctt cacactgacg gaccattact
      121 gctaatggca ggtgcaggaa gtggaaagac gagagtgctg acacaccgca ttgcctattt
      181 gattgaggaa aaagaagtca atccttggaa catcctagcg attactttta ccaataaggc
      241 tgcaaaagaa atgaaagaac gtgtcaatgc gattctcgca tctggaggcg aagatgtctg
      301 ggtatcgact tttcactcga tgtgtgtgcg tattttaaga agagatgttg attttatcgg
      361 gtataaccgg aactttacaa tcatcgattc ttctgaacag ctgaccttga tgaaacggat
      421 actaaaagaa ctgaacattg atccgaaaaa atatgatcct cgaagtatat taggaacaat
      481 ctctcaagca aaaaacagcc tgcagacccc tcaggatttt gcaaaaatgc aaggcagtta
      541 ttacgaagag attgcggcaa aatgttatgc tgcttaccaa aaagagcttc aatataatca
      601 atgtatggac tttgacgacc tgatcatgaa tacgattcga ttatttgaag aacatcctga
      661 ttcactcacg tattatcaaa acaaattcca ttatattcat gtagatgagt accaagatac
      721 aaatcacgca cagtatacgc tagtcaattt attggcaggt agatttagga acctgtgcgt
      781 cgtgggagat gcagatcaaa gtatttacgg ctggcgcggt gcagatatgc agaacatctt
      841 ggattttgaa aaagattatc cagatgctgc agtaattttg ctagaacaaa actatcgctc
      901 cacgaaaaac atccttagcg cagcaaacca agtcattgaa aacaatagca atcgaaaacc
      961 aaaaaacttg tggacagaaa acaaagaagg aaacaagatc acttactacc gtgcagataa
     1021 tgaacgagac gaaacccgtt ttatcgtaga ccggatgcaa gaagagattc gaagcaatca
     1081 tcggaattac ggcgactttg ccatcttgta ccggacgaac gcacaatctc gtgtaatgga
     1141 agaaactctg ttgaaagcaa atattcctta taaaatggtt ggagggcaca agttctacga
     1201 tcgtaaagaa atcaaagata ttttagcgta tttgaatgtg ctggtaaatc ctcaagattc
     1261 aatcagtttt gagcgtatcg ttaattcgcc caaacgaggc atcggaccag gatctatcga
     1321 aaaactacgc agttttgctt ctcttcatga atggccactg cttgaagcag cgcaaaatgt
     1381 ggatctggca aatatcggtg gaaaagcagg acagcaatta ggtgcatttg gagagatgat
     1441 ccaagaagtc actcagatga tcccatactt gacagtgaca gaattgacga aagaggtttt
     1501 ggatcgtagc ggatatctag aagacttaaa aatccaaaat acattagaag cacaagcacg
     1561 catcgaaaac ttggaggaat ttttaacggt cacacaagaa ttcgacaaac aattcgaaca
     1621 gcaaaatgaa gaagacgctg atgcaccaga agaaaaacta acggtcttct taaatgatct
     1681 ggcacttgtt tccgacattg acaatttaga agaagatgct tcccaagtga cattgatgac
     1741 tttgcatgca gccaaaggat tagaatttcc agttgtattc ctgattggtc tagaagaggg
     1801 ggtttttcct ttatctcgag cattgatgga agaaagcgaa ctggaggaag aacgccgtct
     1861 cgcatatgtg gggatcacgc gagcagaaga agcgttgtat ttaacaaatg ctttctccag
     1921 aacattatat ggacggacgc aatataaccg accttctcgt tttgtagaag aaatcgatca
     1981 ggagttactg gaaatcgaag gcatgcgtcc tacgcctaaa aaaacgcctg tattcgctaa
     2041 aaaaacagca tatagctata aacagccaga gacagcggtt gttccctcta aaagtgctac
     2101 tggaggggaa aataacagct ggaaacctgg agataaagtc aaacacaaaa aatggggtct
     2161 aggaacagtc gtccgagtaa gcgggacgtc gaaggatcta gaactagatg tggctttccc
     2221 aagtcaagga gtaaaacgtc ttttggctgc ctttgcgcca attgaaaaag cttagtagtg
     2281 gagggtaaat gatggaacat gaaatgacga ttgacgaagc tgctaaacga gcagaagaac
     2341 ttcgtacaag actgaaccaa tggtctcgcg aatactacgt agaagataaa ccaacagtgg
     2401 aagactatgt ttatgataaa gaatacgcag aattagtagc aatcgaggaa caatatcctg
     2461 atttgatcac gtctgattct cccactcaac gagtaggcgg aaaagtatta gaaggttttg
     2521 aaaaagtcac tcatgatatt ccgctttaca gtttgaacga cgttttcagc aaagaagagc
     2581 tgatagcttt tgatcaacgt gtacaaaaag cagtgggacg tgtagttgac tattgctgtg
     2641 aactaaaaat agatggactt tccgtttctt tgcgttatga agatggaaat ttcgtgagag
     2701 gtgcgactcg cggggatgga actgtaggag aaaacatcac tgaaaaccta aaaactgtcc
     2761 ggtcggtccc aatcaaatta aaagaaccaa tgaacatcga agtaagaggc gaatgtttca
     2821 tgccaaaacg gtcatttgtc caattgaacc aagacagaga agcagaggga aaagatatct
     2881 ttgcgaatcc tcggaacgcc gcagctggtt ctttgcgtca gttagattct aaaatcacag
     2941 cgaaaagaaa tctggacact tttttataca cagtagctga tttcggtcct atgcaagcaa
     3001 aaacccagta tgatgcgcta gaagagctag aaaaaattgg attccacacg aatcgggaaa
     3061 aacgtctatg tcattcgatt gatgaagtgt ggtcatacat tgaagaatat catgacaaac
     3121 gtgtggattt gccctatgaa atcgatggaa tcgtcatcaa agtgaatgaa ttctcgctac
     3181 aagatcaact tggttttact atcaaagcac cgagatgggc agcagcttac aagtttccgc
     3241 cagaagaagt tgaaacgcta atcgaaaaca tcgaatggac agtcggtcga acaggggtag
     3301 taacgccaac agctatcatg acaccagtcc gagtagcagg aacgacagta agccgtgcca
     3361 gcctgcataa cggtgattat attaagttga aggatatccg cttgaaagat acagttctga
     3421 tttataaagc gggagacatc atcccagaag tttcgcaagt cgtgttagat aaacgaccta
     3481 aagacagcga agaataccaa ttgcctacac attgtccagt ttgtggaagt gaacttgtcc
     3541 atttggatga agaagtagcc ttacggtgca tcaatcctaa atgccctgct caaatgaaag
     3601 aaggtttgaa ccactttgtt tcaagaaatg cgatgaatat cgatgggttg gggccgcgcg
     3661 tactagaaca gatgtatgat aaaaaactag tagcagatgt tgctgatctt tacaaattga
     3721 cagaagaaga gttattgacg ttggacaaaa taaaagaaaa atcagcaaat aatattttga
     3781 cagcaatcga caacagcaaa gataattcgg tcgagcggtt gattttcggg ttaggaatcc
     3841 gacatgtcgg agcgaaagca gcaaaaattt tagcagaaca tttcggtgat ctagaaacat
     3901 tgagtaaaag tgactatgag tcgattattg cgcttgatac gatcggtgat atcattgccg
     3961 acagtgtagt cacttatttc agtaacgaag aagtccatga attgatgaat gaattaaaac
     4021 aagcaggcgt caatttcgaa tacaaagggc ttcgaaatgc ccaattgcaa gaagtcgagt
     4081 ctccttttaa agaaaaaaca gtagtcctta ctggtaaact aacacgattt actcgtgaag
     4141 aagcaaaaga gacgatcgaa aatcttggcg ggaaagtgac tggtagcgta tcgaaaaaaa
     4201 cggatatcgt ggtagcagga gaagatgctg gcagcaagct gaccaaagca caagaattag
     4261 gaatcgaagt ttggacagaa gatcaaatgg cagatgcatt ggctaaaagt cgctcagtag
     4321 aagaatgaaa aatgagtttt ttcagacaaa tttcaataaa caatttcaga cacggcactc
     4381 ttgtgttaaa atagagagca aagtttttaa ctcgtttaga aagaaggggt agtatggcga
     4441 ttagtgaaga acaagtgaaa cacgttgcca aattagcaaa acttgcgttc gcagatgaag
     4501 aacttgcaag ttttaccgac caattaggta aaatcatcga tatggttgaa atgctggaag
     4561 aagtagatac ggaaggtgtt ccatttacgt cgaatgtggt tgaaacagta aacgtgatgc
     4621 gacaagatcg ggcgcaagct ggatggaacc gcgatgaatt attgaaaaat gtgccggaaa
     4681 cagaagacgg ctttatcaaa gtacccgcaa tcatcgataa tggggaggct ggcgcataat
     4741 gacagaatta cacaatcgtt ctctagaaga actgcatcat atgcttgtat cgaaagaaat
     4801 cactgtacaa gatttgacaa aaacaacatt tgaacgaatc aaagaaacag agcctgaggt
     4861 cgaggcattc attacattga acgaagaaaa agcattggaa caggcaaaag ctttagatga
     4921 aaaagggatt gatgaaagca acgtccttgc tggtattcca attggtatca aggataatat
     4981 cgtaacaaaa gatatcttga caaccgctgc atctaagatc ctttacaatt ttgaaccaat
     5041 ttatgatgca acggtcatgg ataaaatcta tcaagcagat atgatccctg ttggtaagct
     5101 aaacatggat gaatttgcta tgggtggaag tacagaaact tcttatttca agaaaacaaa
     5161 aaatgcttgg gacccaacaa aagtacctgg tggctcttct ggtggttctg cagcagcagt
     5221 tgcagctgga caaattccag tttctttagg tagtgatact gggggaagta tccgccaacc
     5281 agcagctttt aacggcatcg tagggatgaa acctacttat ggccgtgtct cacgttttgg
     5341 tttgattgcc tttgcttcat ctcttgacca aatcggacca atgacccgta cggtgaaaga
     5401 taatgcatta gcattgacag ctatcagcgg ttatgacgaa aaagacggta cttcttcagg
     5461 cgtttcagtt cctgattttg cagagggact gacaggcgac atcaaaggaa tgaagattgc
     5521 tttacctaaa gaatacttag gcgaaggtgt ggcccctggc gtaaaagaag ctgtattgaa
     5581 agcagcagaa acattccgtt cattaggtgc tgttgtggaa gaagtcagct tgcctcattc
     5641 aaaatacggt gtggctgttt attatatcat tgcctcatca gaagcaagtt ccaatttgca
     5701 acgattcgat ggtatccgct acggttatcg ctctgaaaat gtgaaatcat tagatgatgt
     5761 atatgtcaac tcacgttcag aaggattcgg tgaagaagta aaacgccgta tcatgttagg
     5821 tacattctcc ttaagcgcgg gttattatga cgcttacttc aaaaaagcag gacaagtccg
     5881 tacattgatc aaacgagatt ttgaaaaagt ctttgcagat tatgacttga tcatcgggcc
     5941 atcttcacca acagttgcat tcggaatcgg tgagaacatt aacgatccaa tcacaatgta
     6001 catgagtgac atcttaacga tccctgtcaa cttggctgga cttccaggta tgtctgtacc
     6061 ggcaggttta tcagaaggat taccagttgg attgcagatc atcggtaatt attttgatga
     6121 aaaaacgatg tatcaagcgg catatgcgtt tgaacaagca acagaattcc ataaacaaca
     6181 accagctatt ttaggaggga aagacgaatg aattttgaaa cagtcattgg actagaagtc
     6241 cacgtcgagt taaaaaccaa ctcaaaaatt ttctctccag cgccagctca ttttggtgca
     6301 gaaccaaaca gcaatacaaa cgtgatcgac tggggttatc caggcgtgct gccagtaatg
     6361 aataaagctg cgctggaatt cgggatgaaa gcagctttgg cattaaactg tgaaatctct
     6421 aaagatactc attttgaccg gaaaaattac ttttacccag ataatccaaa agcatatcaa
     6481 atttcccaat ttgatcaacc aatcggtcat gacggatgga tcgaaatcga agtagaaggt
     6541 aaaaagaaaa aaatccggat cgaacgtgtc catttagaag aagatgctgg aaaaaacatg
     6601 catggtattg gcggttattc ttacgttgat ttgaaccgac aaggcacgcc tttgatcgaa
     6661 atcgtttctg aagctgatat gcgttcacca gaagaagcat acgcttatct agaagcattg
     6721 cgatcaatta tccaatttac agaagtcagc gatgtgaaaa tggaagaagg ctctatgcgc
     6781 tgtgatgcca atatttctct tcgcccttat ggacaagaag agttcggtac gaaagcagaa
     6841 ttgaaaaact tgaactcttt atctttcgtg aaaaaaggtt tagcttttga agaaaaacgt
     6901 caagcaaaag ttcttctttc aggaggagaa atccaacaag aaacacgccg ttttgacgaa
     6961 acaacgaata aaacagtttt gatgcgtgtc aaagaagggt ctagtgatta tcgttacttc
     7021 ccagagcctg atatcccgaa attcgtgatc gatgacgaat ggattcaaaa agtaaaagct
     7081 agtttgcctg agatgccagc ttctcgtcgc gaacgctata tccgtgagtt tggacttcca
     7141 gagtatgacg caatggtatt gacacttaca aaagaaatgt ctgatttttt tgaagctgtt
     7201 ttggaaaatg gtgcagatgc caagcaagca tctaactggt tgatgggtga agtttctgct
     7261 tacttgaaca gtgagaaact ggaattagct gagacaaaat tgacacctga gaacttagca
     7321 ggaatgattc gtttgatcgc cgatggaacg atcagctcaa aaatcgccaa aaaagtcttc
     7381 cgtgaattga tcacaaatgg cggagaagcc caagaagtag ttgaaaaaaa tggttgggtg
     7441 caattatctg atccgaacaa actactgcca atcattaatg aaattttgga taacaaccaa
     7501 caatctgtcg atgatttcaa aaatggaaaa gatcgtgcgg ttggcttcct agtcggtcaa
     7561 atcatgaaag caactaaagg acaagcaaat cctggtgtcg tgaataagct acttaaagat
     7621 gaattagcga aacgatagag gctgaaaatg aaaaaagcac gcgtaattta taaccccacc
     7681 tcgggaaaag aattattgaa aaaaaatttg gcggatatcc tacaggcatt agaagaagca
     7741 ggatttgaag ccagcgccta tgcaactaca ccagaaccag attcagccaa gaatgaagca
     7801 tgtagagtcg cagaactagg ttttgatcta gtagtggcag ctggtggcga tggcacaatc
     7861 aacgaagtgg ttaatggaat cgctccgctt gaacaccgtc ctaaaatggc catcatccca
     7921 gctggtacaa cgaacgacta tgctcgagca ttaaaaattc ctagagataa tattaaagca
     7981 gcagcagaag tgataaaaaa acaccaaacc gtaaaaatgg acatcggcct ttctgataac
     8041 agttacttca tcaatatcgc tgcaggtggc tatttgacag aattgactta tgaagttcca
     8101 tcagaactaa agagtatttt tggttatctt gcttatcttg cgaaaggagc agagatgctc
     8161 cctcgagtaa agccaatcaa aatgcgcttg aagtatgatg aaggggaata tatcggaaat
     8221 gcttcgatgt ttttcctagg gctgacgaac tctgttggag gattcgaaca gatcgcgcca
     8281 gatgccaaat tggatgatgg gaagttttct ttaatcattg tgaagacagc aaatatcttt
     8341 gagatcttgc atattgcagc actcatgctg aatggcggtc gacatgtcga tgatccaaga
     8401 ataatctata ccaaaacaag cagtttgttc gttgaaacag aagatgatat caatcgacca
     8461 gtaatgatca acttagatgg tgaatacggc ggggaagcac cgatgcattt ccaaaatctt
     8521 catcaacaca ttgagttctt tgccaatacc gatcagattc ctgatgaagc aatcacaggt
     8581 acagatgaag aagaattaga ggttgtcagc aaagaattcg taaaagaagt cgaacgttta
     8641 acggatgaag atatcgacgg tgacggaaaa atagcagacg aaacacctaa tcaaaagaat
     8701 taatcaaaag ccggtgctga acgtagtaag gtcagtgccg gttttagttg tgtaattagc
     8761 tgatttatag tagagttcag taggattgaa aaataggctt aaaggagcaa aatatgacag
     8821 aacagcaaat agtaaagaaa aacgaacgat atacagtcga aatcgtcgac ttgagttatg
     8881 aaggaatggg ggtagcaaaa atcgatggtt atccattatt tatcgaaaat gcactccctg
     8941 gagaagaagt agaaatacta gtagtaaaag caggcaataa gtttggatat ggaaaagtcg
     9001 aaacatttat tacacaatca cctgatcgtc agcctgtaga aaacaatgca ttacttcgca
     9061 taggtatcgc acctttagcc catttgaagt atgaacaaca gcttgtcttc aaacaaaaac
     9121 aagtagagca tgtaatggcg aagattgcta agatgccaga agttcctgta cttccaacgg
     9181 tcggaatggc agaaccattc ggctaccgta ataaagcaca gatccctgtt cgccgtatcg
     9241 atggacagtt agccaccggt ttttacaaaa agaatagcca tgatctagtc cctatcgaag
     9301 atttttatat ccaagatcct gtcatcgatc aagcaatcaa agtagtcaga gatatcttgc
     9361 aacggttcca agtacgagga tataatgaag aaaaaaatga aggacagctt cgtcacatca
     9421 ttatccgtcg tggtcattat tcccatgaaa tgatggtcgt tttagttact cgtaaagaaa
     9481 aattctttaa atccaaggaa attgctgaag ctattcatga agaattgcca gaagtggttt
     9541 cggtcatcca aaatatcaat gaagaaaaaa caaatgtgat tttaggagac aaagaaaaag
     9601 ttttatatgg cagagactat atcgaagatc agctattagg aaaaacctat cgaatctcag
     9661 caaagtcatt ttatcaagta aatactcaac aagcagagta tctgtaccaa acagccatcg
     9721 agtttgccga gttagataaa aaagacattg tgatcgacgc ttattcagga ataggtacga
     9781 ttggcttgtc tttagcagac agagtggata aggtgtatgg gatggaagtt attccagaag
     9841 caattgaaga tgcacagttc aatgctttaa ataataaaat cgaaaatgtt cattatgaag
     9901 tcggaaaagc agaaaaagtt atgaaaaaat ggcaagaaaa agggatcaaa ccatctgtga
     9961 tcgtagttga ccctccacga aaaggattag atcatcattt tattgaatca gcaatcgaaa
    10021 tgtcaccaga acgaatcgtc tatatctcat gtaatccggc aacttttgca agagatgcta
    10081 agttatttgc agaagcaggt tatgccgtga agaaagttca gcctgtagat cttttcccgc
    10141 aaacccatca tatcgaatta gtcgcttcat tcgttaaagg agaataaaaa tggaccaaaa
    10201 agaaaaaatg aaagaattga tcaaaaagaa acaaggtgga ggccgatcca aacaaatgga
    10261 aacacctaag aatgatcgta aaaacatgag aaaaggccct aaaatcttta ataaatagac
    10321 gtgctaaaga aagactaaga agtgttaact tttgttattg tttcttagtc tttttagtta
    10381 gtctacatta ttttggcgat ttgccaataa ggactgttga gttgtgcaaa atcaaatctg
    10441 ggtcagaatg aacgtcattc tgtctcagat ttttcgttat ttcaatttga ttaactgatt
    10501 gatca
//
LOCUS       DASJST010000098        10358 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJST010000098 DASJST010000000
VERSION     DASJST010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.099"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1459
                     /locus_tag="U4B05_002040"
     CDS             5..1459
                     /locus_tag="U4B05_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEN2051167.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1535..2695
                     /locus_tag="U4B05_002041"
     CDS             1535..2695
                     /locus_tag="U4B05_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEN2051168.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2808..3815
                     /locus_tag="U4B05_002042"
     CDS             2808..3815
                     /locus_tag="U4B05_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2051169.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3839..4864
                     /gene="iolG"
                     /locus_tag="U4B05_002043"
     CDS             3839..4864
                     /gene="iolG"
                     /locus_tag="U4B05_002043"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEN2051170.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4881..5771
                     /gene="iolE"
                     /locus_tag="U4B05_002044"
     CDS             4881..5771
                     /gene="iolE"
                     /locus_tag="U4B05_002044"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEN2051171.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5794..7389
                     /locus_tag="U4B05_002045"
     CDS             5794..7389
                     /locus_tag="U4B05_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HEN2051172.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7405..7719
                     /locus_tag="U4B05_002046"
     CDS             7405..7719
                     /locus_tag="U4B05_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2051173.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8180..8905
                     /locus_tag="U4B05_002047"
     CDS             8180..8905
                     /locus_tag="U4B05_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2051174.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            9038..9880
                     /locus_tag="U4B05_002048"
     CDS             9038..9880
                     /locus_tag="U4B05_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2051175.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            9880..>10358
                     /locus_tag="U4B05_002049"
     CDS             9880..>10358
                     /locus_tag="U4B05_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2051176.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN"
BASE COUNT         3203 a         1804 c         2374 g         2977 t
ORIGIN      
        1 ataaatgaca agatttttaa gaaattatat tgatggtcgt tgggtcgcta gtcgctcaac
       61 agagtttgaa gaagtaatga atccggctac agaagaagcg ctggcgaatg taccgttgtc
      121 tagcaaagca gagacagccg aagcagttaa agctgccgat gctgcgtttc aaacttggaa
      181 aaaagttcct gttcctgttc gcggacgtta tctgttcaag ctgcatcagc ttttaacaga
      241 tcataaaagt gaattggcgc gtttgatcac gctggaaaat gggaaatcat tgtccgagtc
      301 actgggagaa gttcaacgag gaatcgaaaa tgtggagcac gcagcgggaa tcaccaattt
      361 aatgatggga gattctttat caacagcggc gactgatatt gagatcacga attatcgtta
      421 cccaatcgga gtaatcggag ggatcacacc gtttaatttt ccaatgatgg tgcccttctg
      481 gatcttcccg atggctctgg cggcaggaaa tacggtggtg ctgaagcctt cagaaaaaac
      541 accattgttg atggagaaaa ttgttgagct ggttgaaaaa gcaggctttc ctagcggtgt
      601 ttttaatgtt gtctatggtg cccacgatgt tgtcaacaca ttgctgcaag atccactggt
      661 aaaaggcatc agctttgtag gatcaaaaaa tgtcggtgaa tacgtttacc gtgaaggaac
      721 aaaaaatcta aaacgggtac aagcgttgac tggagcgaaa aatcatgttg ttgtgctgaa
      781 agatgccaat attgatcggg cagtcaagga ccttattggc ggagcttttg gttctgctgg
      841 tgaacgctgc atggcagcct ccgtcattgt tttggaagag gaaatcgcgg atcagtttgt
      901 tgaaaaattt aaggctgcgg caaaagcgat caagatcggc aatggcttgg acgaaggggt
      961 ctttttagga ccagtgattc gaaaagatgc acagcagcgg acctttgact atatccaaaa
     1021 aggggtcgat gaaggagcga aactggtact agatggtcgc gaaaacattc cagaaaaagg
     1081 ctattttgtt ggaccaacga tttttgataa tgtcacgatg gatatgaaga tctggcagga
     1141 tgagattttt gccccggtcg catcaatcgt gcgggtaaaa gatttgatgg cagccgttga
     1201 gctgacgaat gaatctgaat ttgcgaatgg ttcgtgtctg tataccaaca acgcatcagc
     1261 gattcgctac ttcagagaaa atgttgatgc tgggatgctg ggggtcaatc ttggggtgcc
     1321 tgcaccgatg gcttggtttg ccttttcagg ctggaagcac tctttctttg gtgatttgca
     1381 tgccaatggc aaagacagtg ttgagtttta tacccatcgg aaagttgtta cagcacgtta
     1441 tgcgcaaagt gagatttaaa gatttgcttc gaagctctgt taaagagctt cgaggattct
     1501 gttataggct ataagccaga gagaggtttt ttctgtgaat attcaactga aaatgcctgc
     1561 caatttaaga attggcggcg gaacaatcca atttcttatg gaagaatcag aacaactagg
     1621 caacaagccg ttaatcatta gtgatacggt tatggaacag accgggcatc ttcaaaagat
     1681 caaggagctg ttgggaaaaa cagatgatca attagcggag tatacagaca ttcaagccga
     1741 accgacgcaa gaaaatgttt atgatgcgct gaagcttttt cagaagcatc aatgtgatca
     1801 tatcattgct tttggtggcg gcagctgtct ggatacagcc aaggtggtag ccgttttggc
     1861 tgctaatcaa ttaagtctcg aagcgattca tgcaagaaat tttgatcagc tgcaagcggg
     1921 cattccgctg attgcagtgt caacaactgc tggaactggt tcggaagtaa ccgatgtggc
     1981 agttattacc aatacaaaaa cagcagtcaa aatgatgatg aaggatctcg ttttcatgcc
     2041 acagatctca atcgttgact ctgattttac gaaaactgta ccaccaaaag taaccgcggc
     2101 aacgggagtc gatgcgctat gtcacgcttt ggaatcttat gtttcagtca aagccaatga
     2161 agtaacgcaa ctattttcac tggaagcgat gaagaaaatt ttgaacacgc tttctcaggc
     2221 atatgaaaat agcgacgaca ctgaagccag agaacaaatg agcttagctt caacggaggc
     2281 ggggattgcc ttttcgaatg cgtcagtcac attgattcat ggaatgagtc gaccggtggg
     2341 ggcacttttt cacattcctc atgggatatc aaatgcgatg ttattgccgg tcttttttga
     2401 attctcaaag gatgctattc agactgagtt agcgacgatc gccagattta tttatccggc
     2461 agccgaagat ttatcgctgg aagaggctgc ggattatctg attaaaaaaa tccaagagct
     2521 ctgtgagcag ctgtcgatcc caacgattcg agattatggt attgatcagc aagcgtttat
     2581 ggcagcctta gacaaaatgg ccgcggatgc gttgaccagc ggaagtccgc aaaataataa
     2641 aaaggtgcct agtcatagtc aaattaagga attatacgta caagcttatg ggtagtaaaa
     2701 ggcggagcaa agagagggct ctacctttta agaataaaag ctacacagga ataattttgt
     2761 aagcgcatac tatgtgtgct taaacaacag aacaggggga taagtaattg agagtaaaag
     2821 taggcgttat cggaacaggt gcaatcggca tcgagcatat cgacaggatc aatcagaaaa
     2881 cacaaggggc ttatgtgaca gcagtctcag atatcaatgt aaaagctgct aaagaggttg
     2941 ccaaacgtgt cgatgctaaa ttctttgaaa ccggagaagc attgatcgct tctgaagatg
     3001 tggatgtcgt tgttgtgact tcttgggacc cgacccatga gcagtatgtg ttagaagcaa
     3061 tcaagcatgg gaaatatgtt ttctgcgaaa agccattagc gatggatgca gcaggctgcc
     3121 gccggatcgt tgatgcggaa gttgcttatg ggaagaaatt ggttcaggtt ggcttcatgc
     3181 gccgctatga ccgcggctac attgaactga aagaagcaat cgaatcgaag caatacggag
     3241 aagcattaat gatgcattgt gcccatagaa atcctagcgc agatgaaaac tacacaacac
     3301 cgatggcgat cagcaacacc gcgattcatg agattgatgt gcttcgctgg ttgctaaatg
     3361 aagattatga atctgttcaa atgatcttgc cgaaaaaaac cagcaactcc catgccgatt
     3421 tgcatgatcc gcagctgctg atctttcaaa caaaatccgg tgtaacgatt gatttggaaa
     3481 tctttgtgaa ttgccgcttt ggctacgata ttaaatgtga tgtggttttc gaaacggcag
     3541 aagtaggaat gacggaccca gcctatacac gaattaaatc ggaagaaaaa aattacacac
     3601 ctgtttcacc agattggcag acccgctttt tggatgccta tgattatgaa ttcaagctgt
     3661 ggatcgattc gatcaaaaat gatgcggttg tcggtcctac cgcttgggat gggtatgtag
     3721 cagcgatcac catgatggct tgtcatgagt ctcgtgaaac aaaaacaaaa gtaatgattc
     3781 aaatggatga acgaccagca ctatacaatg agtaattctc agaaaacgga ggtatttcat
     3841 gaacaacatt agagtaggca tcgtcggttt aggaagatta ggaaaagtac atgcacaaaa
     3901 tttagcaaca ggtgtcagtt gttgtgagct tacggctgcg tgcagtttag ttgaaacaga
     3961 attagcattc gcacagaaga aattaggcgt caagcatact ttttccagct atgaagaaat
     4021 ggtcaaaagt tccgtgatcg atgcagtctt tattgtttca ccatcaggct ttcactgtga
     4081 ccaagtccgc ttggcgatgg aaaatggcaa gcatgtcttt actgaaaaac cattaggcct
     4141 tgaagttgaa gatattaaaa agacccaaaa agtcatcgaa gagcatcctg aacaggtctt
     4201 catgttaggc ttcatgcgcc gctatgatga gtcgtatcag tatgcgaaga aaatggtaga
     4261 agatggcgaa atcggcgaaa tcacactgat tcgttgttat gggatcgatc cgagcgaagg
     4321 cttggaaagc tttgtcaaat ttgccatgaa taacgacagc ggcggcttgt tcttagatat
     4381 ggcgatccat gatattgatt tggttcgctg gtttaccggc caagaagtcg atcaagtgtg
     4441 ggcgattggg aaaaatgcgg cgtatccaga attggatcag gctggtgagc ttgaaactgg
     4501 agccgcaatg atgagcctga gcgataaaac aatagcttta ctagttgccg gcagaaacgc
     4561 agcccatggc tatcatgtcg aaacagagat catgggaacc aaaggaatgc tgcgggtagc
     4621 gcaagcgcct gagaaaaatc ttgtgacgat catgaacgat caagggatca tccgtccatg
     4681 ttcacaaaac ttcccagaac gattcagaga agccttcatt cgtgaagccc aagaatttgt
     4741 ctcatgtatt caagaaaaac gtcaaccagc ggttaatgca gaagatggat tgcaatcaac
     4801 taagatcgcg ttggcttgtc aagaatcatt cgatacgaag agtttagtac aactaaaaaa
     4861 ataagcagga gtgtgagaat atgaccaaac aaattcaatt agccatcgca ccaatcgcgt
     4921 ggaccaatga tgatatgccc gagcttggta gtgaaaatac ctttgaacaa tgtatcagtg
     4981 aaatggccct tgctgggttc accggaacag agattggcaa caaatatcca aaagatccag
     5041 aggtgttgga acaatatttg aatattcgcg gcttaaaggt tgccagtgca tggttcagtg
     5101 cgtttctgac aactaaacct tttgaagaaa ctgccgaagc cttcaaagca catcgggatt
     5161 tcttacatgc gatgggcgct aaagtcatcg tagtagctga gcagggtcat agtatccaag
     5221 ggattatgac agcgccagtc tttgatgata aaccagtttt cactgatgaa gagtggaaaa
     5281 aactggcgga aggattagaa aaattagggg atttggccca cgaaaaggat atggagatcg
     5341 tttaccatca tcacatgggt accggcgttc aaacgacgga agaaatcgat cgtttaatgg
     5401 aaatgactga tccagacaaa gttaaattat tgttcgatac gggacatttg gtcttttctg
     5461 gtgaaaatcc aatcgcggtt tacaaccgtc atcaagatcg tatcaaacac atccatttca
     5521 aagatattcg tcaagaaacg gctgaagaag tagaaaagga aaaagacagc ttcttaaacg
     5581 gcgtgaagaa aggcgtcttc acggttcctg gtgatgggat gattgatttc actccaatct
     5641 ggacagcaat ccatgccagc gactatgaag gctggatcgt agttgaagca gaacaggacc
     5701 cggctaaagc caatccattt gaatatgcag tcaaagccag aagctacatt cgagaggtca
     5761 ccggtcttta atctattgaa aaggagctag acgatgaatc ttttttctat aatcagtttc
     5821 ctagtgattg tcatctgtgt atggttattt gcctattcta gatccagaag tgtcgatact
     5881 tctggatcag aagggttctt catgggaggg cggagcttaa ctgctttgcc aatcgccggc
     5941 acgatcatca tgacgaacct gtccaccgaa caaatagtcg gacaaaatgg tcaaagttat
     6001 catgcaggga tggaagtaat ggcctgggaa gtaactgcta cgattgcgat cgtagctttg
     6061 gctgtgatat tcttgccaaa atattttaaa tacggcatca atacaatctc agatttcatc
     6121 gagattcgtt atgatacggt taccaaacga attatttcga tcttatttat tgtgacctat
     6181 atgacttctt tcttacccgt ggttctttat tctggatcgc tggtgttcaa taagatcttc
     6241 catattgatg agctgctggg cgtccagccg atcgtcgcga tcataattgt agcgatggca
     6301 attggtattg tcggcatcct ctatctgctg atcggcggct tgtcgttaag tgccaatagt
     6361 gactccattt atggagtcgg tctgctggct tgtggtctgc tggtcccaat tttaggctta
     6421 atgaagcttg gtgacggcag ctttgttggc gggatcgaac acgttgtggt aaacacgcca
     6481 tggctgctta actctgtggg atcgatcgac tcggctgttg ttccatggcc gacactattt
     6541 acagggatgc tgtttaacaa tctgtatttc tggtgtacca atcaaatgat cgtccaaaaa
     6601 gcattgtccg gcaagaattt agctgaggca caaaagggcg ctttcctagt cggcgtcttc
     6661 aaaatcttag gtgcgttatt ccttgtcttt ccagggatcg tcgctcgcaa tatttttggc
     6721 gatagtttaa tgtcaaatcc agataacgcg tatccttttt tagtcagtga agtattgcct
     6781 aaaatgatct ttggtatttt cgccgctgtt atctttggcg cgatcttatc atcatttgcg
     6841 ggagctttga acgcgacatc gaccttgttt tcactggatt tttataaacc gcttatcaat
     6901 aagaaagcaa atgataagca aatcgctcgt gctggtaaaa cagtaacggt cgttgtgggg
     6961 attatctcca ttatcgtagc gccattcatt tcttttgcac cggctggatt gtatcagttc
     7021 gttcaagaat tcaatggact atacaacatg ccgctgctcg tgatcattct ctttgccttt
     7081 tattcaaaaa aagcaactgc ttttggggca aaaacgacga ttgccgtgca tatcgtcttg
     7141 tatgcactat caaaaatgct tttccaaaat atccatttcc tttatgtttt gacggcatta
     7201 ttcgttctgg acattatcat tttgatggtg gtttccaaat tgaaacctga tggagaattt
     7261 gtgttgaact cattcgatac aaaggtcgat atcgagcctt ggaaatacac caaggtgact
     7321 gctatagcat tggtagtcat tgttatttta acttatgtgg cattttcacc aattggcttt
     7381 gcaggctaaa aacaggaggg tattatgcat aaggatctat tgattatttg cgagaccgga
     7441 atcagtgcgt cgctgctcgt atcaaaaatg cttgaaacga tcagagccaa aaatctatcg
     7501 tatgagatag attatgcaac gatccgaaac gttgaagaaa aactgaatta taaagactat
     7561 gacgttttac ttttaacgcc gcaggtcgca cgttctgagg aaaaagtaaa agaaatcatc
     7621 aaaaaggaaa aatgtaccgc tcagatcgtt tatatcaaac aagaagattt tcgttatatg
     7681 aatattgaaa acatcattgc aagcatcgaa gaagattaac gagaatcttt gattattgag
     7741 tgtcgaggag gtggtatatg aaaagatggc atgttcgccc cgatcacccc aatatgaagg
     7801 gcggacttat tagaaatacc caatcttgta tttattcaat agggtaagcg gacctcaata
     7861 gccagctcat ccccaaaaag tcgaatggag acacttaaat gagtgtacct attcgacttt
     7921 ttgagactct aagtcaaatc gtgttgttct cattaaatca ctgaaaggaa atgcttcggc
     7981 gtttcctttt ttctttttgt ttttcttcgt gttttgaaaa ttacttacta tttactatac
     8041 gagtagtttc aaataatttt gacatcctaa tcttcaacaa cagaaaaaaa tctaaattat
     8101 aaaatttagg gttgactaaa atataatttt tgtataccat atgatcgtga taatcattat
     8161 catttagaag gagaaagtta tgttagaagt gaaaaagctt aatgtttgtt ataacgattt
     8221 tttagcattg aacgagattt ctttaaaaat acaagaaggt gctttaacag gtattattgg
     8281 tccaaatggt gctggaaaat ctacattgct taaagcaatg ttaaatatta tccctcatca
     8341 gggagaggtc tccattaata atgaagatat caataaaaaa ctggtgaaga ttgcatatgt
     8401 agagcaaaaa gctgatattg attttacttt tccaataaaa gtaaaagaat gtgtatctat
     8461 gggaacatat gccggaatga aagtttttca aagaattaaa aatgcagaat ggcaaagagt
     8521 ttcaaaggct ttggaaaagg tcgatatggg aaaatattct aatcatcaaa taggagaatt
     8581 atctggagga caatttcaac gtgtactttt agcaagatgt cttgctcaaa atgcggattt
     8641 catattttta gatgaaccat ttgtagggat agacttagtt agtgaacgaa taattatgga
     8701 tacgttaaaa gaattgaaac atcagggaaa aacagtgcta attgtgcatc atgatttaag
     8761 caaggtaaag aagtattttg ataatattat tcttttgaat cgaaatctaa ttgctcacgg
     8821 aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa gcctatggag atactatatt
     8881 tattggagag gggggaacgg cttagcctct caatccaata aatatattat ctcaaaagaa
     8941 atgagttaat accaaattaa aataacgatg gttcataggg ttatccaatc aaaaccggaa
     9001 ggttaaaacc ctaactctaa tatacaaggg gaataatatg cttcaaaatt ttattaatgg
     9061 tctgtatgac tttcactttt tacagaatgc tctaattaca tctgtagtta tagggattgt
     9121 ttctggaaca gtaggttgtt ttattatttt aagaggaatg tctcttatgg gtgatgctat
     9181 ttctcatgct gttttgccag gagtagcaat ttcttatatt ttaggtatta atttttttat
     9241 tggtgcgatt gtatttggtc tattagcttc tatcattatt acttttataa aaagcaatag
     9301 tgtaataaaa ggagatacag caattggtat tactttcagt tcttttttag cattaggagt
     9361 tatccttata ggagttgcaa atagctcaac ggatttattt catatcttat ttggaaatat
     9421 tttagctgta caagatattg ataagtggat tacaattggc gtttctgttt tagtgttggt
     9481 tacagttgtt ctatttttta aagaattatt aatcacttct tttgatccat taatggcaaa
     9541 agcaatgggg atgaatgtca gtttttatca ttatctatta atgattctat taactttagt
     9601 agcagtaaca gctatgcaaa gtgtgggaac tatattaatt gttgcaatgt taattacacc
     9661 agctgctaca gcatatttat atgcaaacag tttaaaaact atgatattta tttcggcgac
     9721 agtcggagcg tgctcatcat tgctaggttt gttcattggt tatagtttta acatagcagc
     9781 aggatcaagt attgttttaa cagcggcatt cttatttgta attagttttc taatctcacc
     9841 aaaacaaaaa tttttaagaa agaaggagag aaagttatga tgagaaaatg gaaagcagta
     9901 ctgggaagtc tggggatttt gattgctctt tttatattcg gggcatgttc aacaaatagt
     9961 aaagacaaag atacagtggc ttcgaacgaa aaattaaagg tagtagttac taattcgatt
    10021 ttagcagata ttactgaaaa tatagcaaaa gataaaattg atttacacag tatcgtacct
    10081 attgggaaag atccccacga atatgaacct ttgcctgaag atgttcaaaa aacttcaaaa
    10141 gcagatttga ttttttataa cggtgttaac ttggaaactg gaggaaatgc ttggtttaca
    10201 aaattagtaa aaaatgcgaa caaagaggaa aacaaagact attttgcagc aagtgatggc
    10261 atagatgtta tttacttaga gggtcagagt gagaaaggga aggaagatcc ccatgcttgg
    10321 ttaaatttag aaaacggtat tatttacgct aaaaatat
//
LOCUS       DASJST010000099        10339 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJST010000099 DASJST010000000
VERSION     DASJST010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.100"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..329
                     /locus_tag="U4B05_002050"
                     /pseudo
     CDS             1..329
                     /locus_tag="U4B05_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(231..947)
                     /locus_tag="U4B05_002051"
     CDS             complement(231..947)
                     /locus_tag="U4B05_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEN2051177.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1071..1256)
                     /locus_tag="U4B05_002052"
     CDS             complement(1071..1256)
                     /locus_tag="U4B05_002052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051178.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1483..2756
                     /locus_tag="U4B05_002053"
                     /pseudo
     CDS             1483..2756
                     /locus_tag="U4B05_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2863..3894)
                     /locus_tag="U4B05_002054"
     CDS             complement(2863..3894)
                     /locus_tag="U4B05_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051179.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3901..4731)
                     /locus_tag="U4B05_002055"
     CDS             complement(3901..4731)
                     /locus_tag="U4B05_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2051180.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4728..5543)
                     /locus_tag="U4B05_002056"
     CDS             complement(4728..5543)
                     /locus_tag="U4B05_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2051181.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5540..6364)
                     /locus_tag="U4B05_002057"
     CDS             complement(5540..6364)
                     /locus_tag="U4B05_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEN2051182.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6354..7454)
                     /locus_tag="U4B05_002058"
     CDS             complement(6354..7454)
                     /locus_tag="U4B05_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2051183.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7632..8417
                     /locus_tag="U4B05_002059"
     CDS             7632..8417
                     /locus_tag="U4B05_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEN2051184.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8450..8833
                     /locus_tag="U4B05_002060"
     CDS             8450..8833
                     /locus_tag="U4B05_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN2051185.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8909..9040
                     /locus_tag="U4B05_002061"
     CDS             8909..9040
                     /locus_tag="U4B05_002061"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2051186.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9009..9245
                     /locus_tag="U4B05_002062"
     CDS             9009..9245
                     /locus_tag="U4B05_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2051187.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9323..10294)
                     /locus_tag="U4B05_002063"
     CDS             complement(9323..10294)
                     /locus_tag="U4B05_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN2051188.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3043 a         2436 c         2006 g         2854 t
ORIGIN      
        1 actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt
       61 ctatgctatg attgtgggca gaccttttct gcgaaaacgg acttagttga tgaacatcat
      121 caactaacaa aggaactgaa acaggcgatt ctcatagaac tgtacgaaaa tcaatcacgt
      181 aatctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag
      241 attccacgac attggagccg ctcaaacggc attatcatcg actgtttaaa cgaccaatta
      301 gtcaaacaga aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca
      361 ccttccatat ccagagcagt cgccacatca cggggatgga tgccatccaa attaaaagca
      421 accacgccag tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct
      481 tgtaatttag gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat attctccata
      541 ccaatctcat tcaagtaatc gattgctcct gcaatgttcg gagtaccagc ttcaaatttc
      601 catggcagct ctttccatga actatcgtaa agattgacaa aatctatcat ttcaccgccg
      661 aattcgactg gctccatttg ttctaacaat tgacgtttgc catataatac accgattcct
      721 gtcggcccgc acattttatg cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca
      781 tcaactgtca tatgcggtgc tgcttgggca ccgactggac tatcctcaac tacaacagcc
      841 tgttcaaccc tcagccgttt caagcacaac aaataacctt ctggatctgg tttgccgacc
      901 tgaagatcat cgatcgtcac aaaggaatca attttttcct taatcatcgg atgtgcttca
      961 aaaaaatagt ctagctcccg cagttcagaa ttgagactac tccgattttc cagtgctgct
     1021 gatccgctgc tcaaaatgtt cagcatccac tggtctccca accgcttgac ttatcccttc
     1081 ataaaaggac agttctgctg ccgtcccaac accttgttga tagtctgcaa aaggaatttt
     1141 atagccgaac tgctccagta aataggcttc agtaaatagt gatgaatact ctcgtaaaac
     1201 atccattcac tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc
     1261 tttccccctt acccaaaaca agcaaaagct gtgtcctgct ctctctacag tatcagcata
     1321 aaaactgccg tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt tctaggactg
     1381 atagtaaatc attctatcag tccttttgag cataaaaaaa caaaacatac agaagctccg
     1441 ttagaattga atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatctt
     1501 atcaaactat tactaggaat caaagatgga aacattcaaa tcactcatgt cgagaagaaa
     1561 aaaatacatt caatcaacag tatcgttgtt tctgccacgc tcattaaaac gattcagccc
     1621 tgtcctcagt gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa
     1681 tcgctggtcc ttctcaataa gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa
     1741 cggtacctct gtcgtggctg tcacacctat tttacggcga atacctacat tgttgaaaaa
     1801 aactgtttta tcgccaaaca agtacattat aaaatattgg aggaattgac tgaaaaacaa
     1861 gcaatgacga cgatttctaa acattgcggc gtttcttggt caaccgtttc gcgaacactc
     1921 gcttctctgc tccccatgac aaacgtgaaa ataaattggt tgcctcgctg ttcattggta
     1981 gatgaatttc gttcattgaa aaatcaagtc ggtccctatt ctttttcttg tatggatggc
     2041 gatactggga aattactgga tattcttcct tcacgtaaga agaaagatct catttcttat
     2101 tttatgcaat ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac agatatgaac
     2161 gcctcttatg ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc
     2221 tttcacatcg tcaagcacct cattcgcagt tttaaggata tttgactatg agtcatgaaa
     2281 agcttcgacc gaaacgatcc aatacaagcc aaacactatc gacaagtgaa ggctttatct
     2341 cgacttctga tcaaacgaca ggacacgctt gtttatgata aatggacaaa atggcgtaat
     2401 tttggttggg cctatttaac cgaaagtgaa gtcgtagaac gtctactttc tacttctgat
     2461 gaactacgaa tagcttatgc ttactatcaa gaaattcttc aagcttttta tgacaaagaa
     2521 gcggacatct tctttcagct tgtaaaaaca atgcccaagt cggttccaag agaactgcat
     2581 catataaaaa aagcgtttat aaactatgaa tccggcattc gtttagcctt agagttgcct
     2641 tactcaaatg gcaaaataga aaatctgcat acccacatca aagcattgaa acgaatagct
     2701 tacgggttag gatgagaacc agaattttct taatcaatca tctgatcact tattaaacat
     2761 aaaaaaacat ctaaagcaga aaatcaattc taccttagat gttcttatat cactctatca
     2821 gtcctatttg acaaagagcc agaaatgttt gccgactata cactagcttt gttgtgcaaa
     2881 atgctgagta tcaatttcca cagggattgc tagaatatct ttcagctgaa tgtataaggc
     2941 atcgctaatt ttctcttccc gatcgttcaa ccgatccact aaaatttctt tgccctccag
     3001 cgtttgaaaa acataccgtt gaatgttgcc taaaataaat gattccttcc atagaaccgg
     3061 aatcttaacc ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta aataataaat
     3121 gcagcgattg cccttagcgg aaaactcgtg ggtatactga ttcagctcat accattcgaa
     3181 acgattatag ttgccgataa attccgcaac aaatcggctc tgaggattct gatacaattc
     3241 cttcgaggtg gaaacctgag ccacccgccc agcttccatc acaaaaatcc gatcactgat
     3301 tcgcatcgcc tcttcttggt catgagtcac aaaaatcatc gtcatcccta gcgattgctg
     3361 gatagcgcgc aaatcccgct gcagctgttt tctaattcgg gcatccaaag cacttaatgg
     3421 ctcatccagc agcaggactt tcggttcagt gaccaatgag cgagccagtg ccacccgttg
     3481 tcgttgaccg ccagaaagaa actggacatt tttatctgcc tgctccgtca tatccaccaa
     3541 tgatagcatc tcaaaaacct tttcctgaat cacctcagga ctcagcttct ttaccttcaa
     3601 accaaaggct acattttcaa aaacagtcat agttggaaac aatgcatact gttgaaaaac
     3661 aaagccgata ttgcgatcct tcgtaggctt atcggcaata ttctcattgt ccagcacaat
     3721 ctcaccgctc tttggttgtt ccaatcccgt gatacagcgc agcaacgtag actttccgca
     3781 cccgctggga cctagtaaag taaccagctc acccttttca atctgaattt catcaatagc
     3841 taaaatctgt ttttcatcaa aaaacagatt gagatcatgt atttctaaaa acattttccg
     3901 tcacaactcc tttaagggct tctttttcga ctgcttctct tccctcgtct gcttaaggga
     3961 cagctgttgc ttttgtcgat aagtcagcat aaaggtagtt aaagcaattc ccaccataaa
     4021 cagcaaatac aaaaccatca cagcgctagc caaatggccg ttttcgttca tccgccgcag
     4081 catatagatg cgtaccgtct gaaatcgacc gccaatcaac aggttcgtca gcatgtactc
     4141 cccaaaaacc gaagaaaaaa tcatcagaga aactagggtc acaccgatac ggatgtttgg
     4201 aaacagcacc tggataataa tcgtagacat cggggcgccc aaggtttctg ctgcatccac
     4261 taattccttc agattaatta agcgcatctg gttgttaaga ctaagataga caatcggcaa
     4321 cgccgtaata aacaatgcac ctaccaacac tacaaacatt gggataccag tttcagcata
     4381 cgttcgaagc aaagccgtta ccaaaatcac tcctggtaaa gcatacggca gcaggagcag
     4441 cttttccagc caacgattca aacgagggaa atacaaatga atccaaataa tggtgggaat
     4501 catcaacaac agaatcgtca ccaagaccac acctgctaat aaaagtgagc gacccaccgc
     4561 agcaataaat tccctgtccg tcagcagctg ttggaaccat tgcaaggtaa aatcactagg
     4621 taaaagcgat tttgaccatt tagtggagct ggcatacaaa atcgttccaa taatcggcag
     4681 caagtaatac aggacaaagc agaacaaaat cggttttatt aactttttca cgtctgcacc
     4741 accttgcctt tcttttgaat aatcaacatt gccgttacca gcaggacacc taaagcgact
     4801 gccaacgcac tgcctaaatt aggcttggcg aacacatctc ccgacactaa agccgcaatt
     4861 cgaatcgtta aaagatttac attactgctg gtcagggcat acgctgtttc ataggtcccc
     4921 atgccattag caaataagat gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa
     4981 gccaccttga accaagcata cgctgatgaa gcgcccagca attgtgcgga ctccagccat
     5041 tcctttttca cttccttcat gctgggatac aaaaacaaga cccctaacgg aatttggaaa
     5101 taggtatacg tgatggtcaa tccccaccaa gaatacaaat tgaagtccgc tagaaatggc
     5161 aaaatcattt tcagcacacc tacattcccc aacaaaataa taaaagagaa tgccagcgga
     5221 atcccagcaa aattagctgc caaattggaa acaaaggtga ttttctcctg caatttagtc
     5281 gatagattca ttaagcataa acataaaata aacgtcccga ttaacccaat cacactggag
     5341 ctgacagcaa tcagtacgct gttaacaaag gcctgtgtgt aatatttgtt gctgaaaata
     5401 tcactgtaat tggccaatgt ccactggtta gttaaatcac tgtgcaagct gcacagaacc
     5461 ataaaaagca gcggcagcag taaaaatgca cagaagataa aggaggcagg aaccagaacc
     5521 aagcgttttt tcatctcttt catcccctca tcaattcttg aatgtcctgc attttctctg
     5581 acggtgcaat tcctaataac cagcaaaaca atggagccgc cagcagctgt ggtacagttt
     5641 gttccttcag cttttggcgt ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag
     5701 ccaagctgcc gccatgcagt ccgtgcgcat ccatcccgtg atccgcagtc accacgactt
     5761 gataaccttt ttgcagccat tcaggaaggt aaatagccaa taatctgtcc gcctgattaa
     5821 ccgctgctgc atattcatga gaatcggcgg taaatttgtg accgacatca tcaatattca
     5881 tcgaatgtac gaccaaaaag tctggctgat actgattcac taaataatgg gcatccgcaa
     5941 ataaatgcga atcagggtaa gtgtcctcac tataaaagat gccatgctga atcagtcgct
     6001 cctcctgatg ctggatgcga tcaaccaaac gattataggg ggcttgattg tacaactcgc
     6061 tgtaccagtg gtaagcggcg gctcctgttt ttccgccagc ttccttaact aattcaaaca
     6121 gcgaccactc tttggatcgc tgagcatggt aattactata gatgccattt tcataggctg
     6181 gaacgccggt catcagtact tcgtataacg gacgtgaatt gctgggcagc tcacttttta
     6241 ccttcaataa gcttgcttgc tggtgctcaa ctaaatgatt catgaaccca agctgggcga
     6301 cggctgtgtc gtagcgacag ccgtctagta taactacaat cagtttgtca agtctacttt
     6361 gcataggtca gtacctccgc ctgccaaagc tctggaagct ctacagaggt cttgttccaa
     6421 gcttcattgt cttcaactgg caccgccgct tcataagcat cctccggcag taatttttca
     6481 gctacttctt taggcagctc taccttgcga ataggtcgcg catagccctt tgccaaattg
     6541 atttgccctt catcagataa aatatattcc cgtgctagct ttgccgcatt gggatgaggg
     6601 gcatttttat tgattaaggt tgtatacccg ctaatgatgg agccgtctgt tggcacagtt
     6661 acctcaaaac gctcggagtc aatttggtca cgataattta gtccattgaa gtcccaaaca
     6721 actgccacat cgatttcacc tttttcaaga tttgctacgc tggcatcaac ggtggacaaa
     6781 cggccttttt ctgccatttt tttgtagtaa ttcagacctg gctgaatatc cttttcgtca
     6841 cctccgttgg caatcgccgc agccaataca gcaaattgtg cttggttggc aatcgtcaca
     6901 tcccccacgc taacttgata gtcaccctct tgcagctcct tccagctagt cggtgcttgc
     6961 ttcacattct ctttatcggt gataaaggaa atggttcccg tatagcttaa caaccaatcc
     7021 ccatcatcat cctttgccca atccggaatt tcctcccaat agttggtctt ataaggtaag
     7081 gttaactctt tgctttcagc caacggaccg aagctgatac cgacatcacc aatatcggcg
     7141 gttccatttt ttccttctga ttcaaactta gccaattctt ctgcactgga catatccgta
     7201 tccacgtgac ttaagccgta ttctttttca aggtcgttcc aagtgcctac ccaatttgcc
     7261 caagtatccg gcattcccaa gctggcaatc tctccttctt ccttggcttt ttctataatc
     7321 gtatctaaag agtcgtctgc cgcagccgat tgcttcgttg cttcacctga gccgcagcct
     7381 gtcagcagga tacttactgt tgctgcacaa aataaaccga tagtcatgtt acgaattttt
     7441 tcattcattt tcattcattc ttctcccctt tcgataattc cattctaaca aagctttgta
     7501 atgagagaat cagtttcttg aaaaggtttc atgaacaatg taaaggttgt atatggtttg
     7561 taaatttcga cgaattcccg ttaaccttct aaagcttttg cggtatgata gtagaaaaaa
     7621 ggaggctccc tatgcagtat tatgatcagc atctccacac gtatttttcc tttgattcag
     7681 aggaaaaatt tgaaaactat ttagcctatc agccggagtt tttcgtttct acggatcact
     7741 ttgatttaaa caatccatct accggaaaag atgacattcc ggattatcca gcctatataa
     7801 acaaactgga ctctttaaaa gaaacctatt ccaccaaatt tctgcgaggt atcgagattg
     7861 gcgttgttcc aggacaagaa gagcggatcg tggattacct cgctcagcat ccttatgatt
     7921 taaagctggt aagtattcat caaaatggcc gctttgatta tatggacgat gtggttttaa
     7981 ccaaagatcc cattcaaact accaaggaat attttgatca gatgttgcag gtcttgacgg
     8041 attttccaga aggagatatt ttgactcact ttgattatgg attacgccgc ttcaccttca
     8101 cagcggaaga attcgcggag cattttgagg ctcagctgct aacgattttc aaaaaggttg
     8161 ttgaacgaga attggcgatg gagttaaatg ccaagagctt cttgaaatac ggcaacgaag
     8221 acctttaccg ttatgccgtt cctttgtatc aaagtgtcgg cggaaagctc tttaccttag
     8281 gctcggatgc tcatgttgcc gaagattatc agctgggctt taaaaaaatg agtcagctgt
     8341 tggcagactg tcaggtagaa aagttgctag tcattcaagg aaaagaacgc cgcctgacgc
     8401 cgctgccgat tttgtgattc ctatataata gaagaaagtg gtgaaaaaaa tgagcaacct
     8461 gtgggactac aatcaagagg cacccatcca ttatttaatt gcgcgacatt gggatgcgtt
     8521 gaaaattgag gcggtttgcc gatccttgtt agcagccgtt ccaaaacaac aactggaaaa
     8581 ttttttggtg gctgacagct tgcagcggga aaaagttcag gcttattttg cagcctttaa
     8641 agatcagcct ttggaatatt tgcatgcgca gtttcatctt ttttatcaag tagccgcccc
     8701 agatgactac aacgatttac gaggacagct gcaattgacc tttcaagcgg atgaaaccgc
     8761 ctataccgtt ttattgggaa tggctcgcct tggtgatcag gcgaaggtcg agtggcggat
     8821 tttcgatatt tgatggcagg atcaaggcgt ttcagttgca aaccttcctc tctttcaatt
     8881 agaaaaaccg cagcaggaag aagtttgtgt gctggtcacc gatgctggtg aaattcgtct
     8941 ccgcttattt cctgaccaag ctcccctggc tgtagccaac tggcgctccc tagctgagca
     9001 aggcgtttat gacaacacac ctttcggctc gtgtcattaa ggaatttgtt attcaaggtg
     9061 gcgctttgga tggttctggt gatgaagctg tttccagcta tgagggcttt ttcgctgatg
     9121 aagtgcatcg cggactttat cattttaatg gcgccttggc cttaggggac cacggccccc
     9181 acaccaacgg caaccagttt tttattgtgc aaaacacaaa ggcgcaggct gacctgctaa
     9241 tgtaaagcaa gcatacttgg agcttggagg actaccggag ctggatggtc gttatactgt
     9301 ttttggacag gtataccaag ggttagaaat cgtagcagcg atcgcagctc agccgacaaa
     9361 cagtgaagat gtaccgattg agccagtgtt cattcgacaa attaacttcg aaaaaacatc
     9421 gtaaaaagac actcactgac ttcagcaaaa gctctattct tgagctggct gcagctagtg
     9481 agtgtctttc atttatccat aagataaagc ttggattcaa taaataaata aatgttttgg
     9541 ttaaagctgt gaccagcaaa tgaggaaccg tgcgagccag ttgatctgcc aatgcaaaca
     9601 tttctttagg tgaaggcgct ggcgtgtctg ccaacggata ctccagtccc aatgcttggt
     9661 aaccgtgcat accatagcag tggtacggta gtaattctaa cggtacgaac cgatcaagat
     9721 ctgccacaaa acaagcaatc gtcagcagct cggtcaggtt catattgata cctggaacca
     9781 caggaactcg aattcgcact tcgcagttag gatactcaat taaaaagcga cgaatattct
     9841 tcaaaatttg ttggttatct aagccggtcc attgtttgtg gagcttgctg gtaaacattt
     9901 ttacatccac aaacaatgta tccacatagg gagctacctt ttggatttca ttataagcac
     9961 cacaaaagct ggtttccagt acggtattga ttccaatgta tttcgatttt tgcaaaattt
    10021 ctttagcaaa atccgcctga accagtgcct cgcctccgct aatggtcact ccgccatccg
    10081 agtgaaagta aaaaacagca tccttctcaa tctcatccat tacctcttca gcggtcatgg
    10141 tctggccaaa tcctttttca atctgcttgc tttgtgattc gggaatcgaa caccaggcac
    10201 agcgtaatgg gcagcccttt aaataaacaa cggtccgtaa accagcacca tcatgtagag
    10261 aaccctgttc tattcttaat actgttcctt ccatatagat ttttgaatta cacactagtt
    10321 taaactaaaa cacacaagg
//
LOCUS       DASJST010000100         9901 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJST010000100 DASJST010000000
VERSION     DASJST010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.101"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="U4B05_002064"
     CDS             278..787
                     /locus_tag="U4B05_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2051189.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="U4B05_002065"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="U4B05_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2051190.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="U4B05_002066"
     CDS             2322..3302
                     /locus_tag="U4B05_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2051191.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="U4B05_002067"
     CDS             3424..4842
                     /locus_tag="U4B05_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEN2051192.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="U4B05_002068"
     CDS             4913..6382
                     /locus_tag="U4B05_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2051193.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="U4B05_002069"
     CDS             6392..6859
                     /locus_tag="U4B05_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2051194.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="U4B05_002070"
     CDS             6895..7767
                     /locus_tag="U4B05_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2051195.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>9901
                     /locus_tag="U4B05_002071"
     CDS             7848..>9901
                     /locus_tag="U4B05_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2051196.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLS"
BASE COUNT         3430 a         1434 c         1977 g         3060 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 c
//
LOCUS       DASJST010000101         9873 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJST010000101 DASJST010000000
VERSION     DASJST010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.102"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(22..267)
                     /locus_tag="U4B05_002072"
     CDS             complement(22..267)
                     /locus_tag="U4B05_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN2051197.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="U4B05_002073"
     CDS             complement(381..959)
                     /locus_tag="U4B05_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2051198.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="U4B05_002074"
     CDS             complement(1036..1566)
                     /locus_tag="U4B05_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN2051199.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="U4B05_002075"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="U4B05_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN2051200.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="U4B05_002076"
     CDS             complement(2423..2818)
                     /locus_tag="U4B05_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN2051201.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="U4B05_002077"
     CDS             complement(2815..4227)
                     /locus_tag="U4B05_002077"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN2051202.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="U4B05_002078"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="U4B05_002078"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN2051203.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4846..5085)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5082..6542)
                     /locus_tag="U4B05_002079"
     CDS             complement(5082..6542)
                     /locus_tag="U4B05_002079"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN2051204.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="U4B05_002080"
     CDS             complement(6712..7869)
                     /locus_tag="U4B05_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN2051205.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="U4B05_002081"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="U4B05_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN2051206.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="U4B05_002082"
     CDS             complement(9336..9863)
                     /locus_tag="U4B05_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN2051207.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DASJST010000102         9810 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJST010000102 DASJST010000000
VERSION     DASJST010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.103"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(33..2153)
                     /locus_tag="U4B05_002083"
     CDS             complement(33..2153)
                     /locus_tag="U4B05_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2051208.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="U4B05_002084"
     CDS             2383..3561
                     /locus_tag="U4B05_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN2051209.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="U4B05_002085"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="U4B05_002085"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN2051210.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="U4B05_002086"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="U4B05_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN2051211.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="U4B05_002087"
     CDS             complement(4914..6167)
                     /locus_tag="U4B05_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2051212.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="U4B05_002088"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="U4B05_002088"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN2051213.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="U4B05_002089"
     CDS             7745..9091
                     /locus_tag="U4B05_002089"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN2051214.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="U4B05_002090"
     CDS             complement(9399..9785)
                     /locus_tag="U4B05_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2051215.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1782 c         2011 g         2891 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactagt gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttgtctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat
//
LOCUS       DASJST010000103         9797 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJST010000103 DASJST010000000
VERSION     DASJST010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.104"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            25..351
                     /locus_tag="U4B05_002091"
     CDS             25..351
                     /locus_tag="U4B05_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2051216.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            419..1132
                     /locus_tag="U4B05_002092"
     CDS             419..1132
                     /locus_tag="U4B05_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN2051217.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            1442..2294
                     /locus_tag="U4B05_002093"
                     /pseudo
     CDS             1442..2294
                     /locus_tag="U4B05_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            2415..4280
                     /locus_tag="U4B05_002094"
     CDS             2415..4280
                     /locus_tag="U4B05_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2051218.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            4328..5737
                     /locus_tag="U4B05_002095"
     CDS             4328..5737
                     /locus_tag="U4B05_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN2051219.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            5845..6546
                     /locus_tag="U4B05_002096"
     CDS             5845..6546
                     /locus_tag="U4B05_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2051220.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            6705..7184
                     /locus_tag="U4B05_002097"
                     /pseudo
     CDS             6705..7184
                     /locus_tag="U4B05_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            8032..8607
                     /locus_tag="U4B05_002098"
     CDS             8032..8607
                     /locus_tag="U4B05_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN2051221.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            8607..9047
                     /locus_tag="U4B05_002099"
     CDS             8607..9047
                     /locus_tag="U4B05_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2051222.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            9063..9764
                     /locus_tag="U4B05_002100"
     CDS             9063..9764
                     /locus_tag="U4B05_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2051223.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         3289 a         1585 c         2163 g         2760 t
ORIGIN      
        1 aaatgattgg aagaaggcaa gcaaatgaat agcatggaaa aagttaacca gtttagagat
       61 gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg ctatttctat ttctttggaa
      121 gcatctgaat tgttagaact ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa
      181 ttagaacgaa tcaaagaaga actggcggat gtattgatct attcatacat gatggcagac
      241 aaccttcagc tggatctaga tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa
      301 tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca cagagtttta gaaatgtgtc
      361 attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat
      421 gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc ctcttgttcc tcttgttatt
      481 agtaggacta gctttgattt tcaatgaaca gatcaaggat tatcttgtca gagagacagg
      541 agataaatat gctatagcga atgtaacaaa agaagatctg aaaaagaata acgataaaga
      601 tgtcagtttt gactttgatg cggtagaacc aatgacgaca gaaggggtca tgaggtcgca
      661 gatgagagga acagatctac cagtgattgc tagtatcgca gtcccttcag tttcaatcaa
      721 tttaccgatt ttcaaaggct tggataatac gtcactactt tacggagcag gaacattatc
      781 tccagatcaa gaaatgggaa aaggaaatta tgccttagca agccatcgtg caacgaaccc
      841 tgaattgttg tttacaccgc tggaaaattt agagatgggt gccaagatct atttgacaga
      901 tttagaaaat gtgtatacat acaaaacatt ttttaaagaa aaagttgctc ctacagatac
      961 ccaattactg aatgaagtcg aggaaaaaga aattgtcaca ctaattactt gtggcgacat
     1021 ggacgccgtc acgcgactag ttgtacaagg agaactggaa agtgttacac cgataaaaga
     1081 tgcgacagat gatatgagat ctgctttcaa tctagagaca aaaacatttt aggatactgg
     1141 gacataagtc gttacagatt aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa
     1201 tttgctgaaa aaaagaggaa cgcaaagagg tgcatctctg atttggttgt tttctagata
     1261 gacttgtgtt ccagtttttt tattttttat atgaaaaggc ttgcataaat aaaaattcga
     1321 tggtatgatg attttgaata agaaatggat tgttactgta cggcaggcaa aaccagaatt
     1381 cagctaagaa acagtaggcg aattctaggt ttttttattt ttcttgagag ggcgaagcaa
     1441 aatgaaaatt gataaaattt tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga
     1501 agaagtcgtt tgtatgggaa aaggactagc ttttcaaaaa aaagattggg gacgagattt
     1561 cgccagaagc ggtccaaaaa gaatttattt taagagattc ttttgctaaa aaccaattcc
     1621 agcagctgat ggctgatatt ccggcagagg agatcgaatg ggtcaaacaa gtagtcgaac
     1681 tggctgaaga taaactaaaa gttgaatttt cgcctaatat ttatctgaca ttgaccgatc
     1741 atcttcatta tgcgatcaca cgagcaaaag aaaatatcca actgcctaat ccattattat
     1801 ttgaaacaaa aaaattttat ccgaaagaat atcaagcagc gaaagaggct cttttgttcg
     1861 tgaaacaaaa gacgggtgtg gaactttctg aggatgaagc cggttttatt gcctttcatt
     1921 ttgtgaatag ccaacaagga aacgaagaca tgcaagtgac gatgaccgct accacaatgg
     1981 taagagatat actgagtatc atcagtaaat tcttcggcat cgtgttcgat gaagaatctt
     2041 tgaattatca gcgtatcatt actcatctgc agttttttgc ccagcgttat ctcaaagggg
     2101 aaggatctga cgagcaagat gaatttctct acgcattagt tcaaggaaag tatccaaaag
     2161 cgtttcattg tgtcgaacgg atcaatgagt atcttcgaca aacgcaaaaa caggaaatgg
     2221 ggatcgcaga gcagatttat ttaacgattc atattcaaag agtagtaagt gagaaaaaaa
     2281 gcatccaatc ataaggattg ttactgtatg gcaggcaaaa cctggattga aaaaataggt
     2341 tcacgaatgt ttgtgactgt ttttttcaat ctgggttttt attttttaaa taaaaaagaa
     2401 agaaggaaaa aacaatggat aatcaagcag ttggtcgtcg tgtatgggaa gcggtaggag
     2461 gacaaaagaa tgtcaaaagt ttagtacatt gtgctacgcg gttgcgattc agattaaaag
     2521 atgaatcttt agcagatacg caaaagttaa aagaagaccc ggacgtgatc caagtcgtac
     2581 aaagtggcgg tcagtatcaa gtagtgattg gcagtaatgt agcagatgtt tatcaatcga
     2641 tcgtggatga ggaaggtctt gcagtttcgg aggatggaaa agaagagtcc aaaaatattt
     2701 tgaatcgtct gatcgatatt atctctagta tatttacacc ttttctaggg gcgatggcag
     2761 ccgccggggt attgaaagga ttcttatcat tggcaacggt gatgggctgg ctgacagctg
     2821 atagcggtac ttatcaaata ttgtttgctg cagcagatgg tgtctttacc ttcttacctg
     2881 tcatgcttgc ttttacagca gctaaaaagt tcaaagccaa ccagttttta gcagtagcaa
     2941 ttgctatggc acttgtgtat ccagctatca cagcagttgc tggtgcggga gaagctatca
     3001 gtttctttgg gatcccagtg attctttcac catctggtta tacttcttct gtcataccaa
     3061 tcattctagc cgtatgggtt caaagtaaat tagagccatt cgtaaaaaaa gtgattccgc
     3121 aatttctgca aatgatttta gttccgcttg tcgttttagt agtgatggtt ccattaacat
     3181 ttcttgcttt aggaccaatc gggacggttg ccggtaatgc tttaggcggt ctcttcaact
     3241 cgatttatgg atttagtcca atcgtagcag gtctaatcat gggaagttta tggcaagtat
     3301 tcgtgatgtt cggcatgcat tggggattcg taccgatcat gtttttgaat atcgaacaat
     3361 atgggtttga tgtattgatg ccaatgcttc ttccagccat cttggcacaa ggtggggctg
     3421 ctttagctgt agcattgcga acaaaagata cgaaacttcg agcattaggg atttcttcta
     3481 cagtgacttc gttatttgga atcactgaac caactgtgta tggtgtgaca ttacctttga
     3541 agaaaccatt cattgccgct tgtatttctg gaggtattgg tggagctatt atcggatttt
     3601 ccggagtaaa agctttctcc agcagtttgg tcagcttatt gacgattccg actttcatta
     3661 atacggtgga tggtgtagag tcaaatgtga cagtagcagt aatagctaca ggaattgctt
     3721 ttgttcttgc attcgtaggt acattgattt taggatttga cgaacaaaca caagacaatc
     3781 aattagaaaa caaacatgcg aatgcaggag aaccgattac ttcagcacgg cataccttaa
     3841 aaagtccttt aacgggtaaa gtactgccat tgagtgaagt accagaccaa gttttttctt
     3901 ctggcgtgat gggaaaaggt atcgctattg atccagaagt aggagaacta gttgcacctg
     3961 cagacggaga gatcacgact atttttccaa ctggacatgc tgtagggatc acaacgactg
     4021 acggagcgga gattttgatt catatcggga tggatacagt agaattaaat gggaatggct
     4081 ttgagatctt agtcaaacaa ggggatctcg tcaaagcagg agatttattg attcgttttg
     4141 acatcgaagc aattagagca gctggctata gcgtaatcac tccagttgtc attacgaata
     4201 ctgatgcatt tgccgatata cttgaattag accaaaaaga gattattgca aacgaagatg
     4261 ttctggcaat tgtaaaataa ggaggaaatc aggatgcaat cacgtttttc agaaaaattt
     4321 ttatggggtg gagcaactgc tgccaaatca attagaaggg gcttatgatc gagacggaaa
     4381 aggactatcc gttgcagatg ctatgcctgg aggtaagcag cgctttgcga ttatcggcag
     4441 tgaagaattt gactggacga ttgatcaaga aaaatatatt tacccgaatc atcgaggaat
     4501 cgatcattac gatcgtttca aagaagatct tgccttgttt gcggaaatgg gattcaaatg
     4561 ttatcgtttt tcgattgcct ggacacgaat ttttccaaat ggagacgaag gtactcctaa
     4621 cgaagcggga ttagaatttt atgatcagct gattgatgag tgcttaaaat atgatattga
     4681 acctgtgatc acgatttctc attacgaaat gcctcttcat ttggcaaaag aatacggtgg
     4741 ctggaaaaat cgaaaactga tcgacttcta tgaacgattt gctcagacag tattagagag
     4801 atacagcagt aaagtcaaat attggatgac tttcaatgaa atcaattcgg cctttcactt
     4861 tccagcttta agtcaaggat tagtaaaaag caatggagca ggagagtacc aaaatatttt
     4921 ccaagcatgg cataatcaat tcgttgcaag tagcaaagcc gtaaaaatcg gccacgaatt
     4981 gcgttcggat atccaaatcg gctgcatgat tatctatgca acaacttaca gcatcgatgc
     5041 caatccagtc aatcaggcag caacaatgat ccaaaaccaa gaattcaatt ttttctgcac
     5101 ggatgtccaa gtacgtggag aataccctgc atacacagcg cgtacccata aaaaatatgg
     5161 agtcgatccg gaaaaattag agcaaacgga aaaagacttc cagttgctaa aagaatatcc
     5221 agtggactac atcggattta gttattatat gtctacggca atcaatgaga ctgatccaca
     5281 agcagcaact tcagaaggga acttgctggg aggagtaaaa aatccgtttc ttgaggcaag
     5341 tgaatggggc tggcagatcg atccagaagg gctgagaatc gcattgaatg aattatacaa
     5401 tcgttaccag aaaccattat tcattgttga aaacggtctt ggagccattg atcatatgga
     5461 agaggatggc acgattattg atgattatcg gatcgattat ttacgtcgtc atatcgaagc
     5521 gatggctaat gcagtagcag acggtgtaga tttgatgggc tatacgcctt ggggatgtat
     5581 cgatcttgtc agtgcatcaa caggagaaat gagtaaacgt tacggcttta tctatgtgga
     5641 tctagacgat gaaggaaatg ggacactgaa tcgatcaaag aaaaaatctt ttgattggta
     5701 taaagaagta attgcctcaa atggcgtaaa cttgtaatta aagcagctga atgaaaaaga
     5761 acacacttct cacatgtgat ttgtgttctt tttttatttt ttatgtgcta ttcttttagt
     5821 aatagtaaag caaagggtga cgttatgaaa aggacacgtg tactatactt ggaagtcgca
     5881 gataaaatta aagaagatat tttctcagga aaatatccag tcggttcgat gttgccgaca
     5941 gaaagtgaat tagaagaact gtttcaagtc agcaaaatca ctgtccgaaa ggcaatcgaa
     6001 ctgctggcat cagatgaata tgtcgcaaag aaaagtggtc gtgggacgac ggttctaagc
     6061 aatcgtcctt acaatcgatt atccaaagcg gcaagcttta cgcaaatctt ggagaactcc
     6121 cgatatgaag tccggaaaga aacgctggat ttgcaattgg tccatataaa gaaggaagat
     6181 cctttgtttt cctttttcgg tgagcaagct gtttgtttta agcgcttata ttatttgaac
     6241 aatcagcctt atatttattt tgttcattat cttccacaag aaatgatgca attcagcaga
     6301 gaagaatttg gcaaagaatc tttgtatcgt ttgctgacga aaaaaggata tactattgac
     6361 tcattttctg atgatttctc ggcagtattt ttaacagaag aagaacaagg aattctgaaa
     6421 acagacgcac agatcggcat caaacggata cggaaatcgt ctacgcaaat tggggaaatg
     6481 attgaatatt cggaggcggt ctaccatacg gagctgcatc cttaccatat tgattatgaa
     6541 acataaataa aaaaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca
     6601 aacaaaaata gcttctcata tttttcgttt ggttaggctt attaccgcag atacacggct
     6661 gtttctggat taccatttat tctggataag ggggtgtggc acgattttca gaaggaatct
     6721 atgaaccaat cactgtattg attttagctt cgatggtaac cccattcggc ttgacgatcg
     6781 cttatttctt agggaaaatc attcgtaaaa atatactcaa tcgtcaagaa atcgatacat
     6841 tgaagaccgc ttttcctatg gggatctgtc agatcacaga gggatgtttc cctatcgtat
     6901 tgaacgactt ggcacgcaat gtcatagcaa cgggagtcgg tgggacagta ggtggcggat
     6961 taagcatgtt ttggggcgca gatagccgta ttccggcatc gggaatgttt gcagtcgcta
     7021 cgatgaccag accatgggca ttcattggtg cattgttagc tggttctttt gtcacaggaa
     7081 taaccatttt attattgaaa aaaccagtag atccgaatgc agaaatcatc caagaagaaa
     7141 aagaggaaga agacatatct tgggatgatt tgacaattag ttaggagagg cagaatggat
     7201 tacaagaaaa aagtacaata gatgtacctt gagacaggaa tggtatgaaa gaatcagagt
     7261 tagaaagaat cgaatataca gattttggtt taggagcgat cgaaactgtc cagagcatcg
     7321 gcaccctcct tgaaatgacg atgaaggaaa aatgagaacc tttcgttgtc gaaagggtct
     7381 ggtatacctt atgtcgaagg aaaggaaaca aaaaaacttt cctgtactat tccaaaaagg
     7441 aaagaaagtt attatacagt aaaaaaagaa attattcttc atgctagaga tcagtacacg
     7501 ttcgagccca atatcaaaca ctagttccag gcaggcgcaa caggtgcggt tatcactgaa
     7561 ttttcttcca gcagtgacga ttcaagtgat atttttacaa atccagctat ttatcgttaa
     7621 caaaaagatt tttaggagaa gctgatgaac aaaaatgatc aaattttgat cgtttagttc
     7681 gtcggctttt ttatttagag aaacatcatt tatcagtgaa acataacacg agaacaaaat
     7741 gattttttcg ttaataaaag aaacaagtat ttgttgttta aaataataca cgttattggc
     7801 atataggacg aaatcccagt atcttctata agtaagtcat ttcatatcga tgaaaacacg
     7861 ttatttattt ttgttttatc tataaaatgg ctgaaaaatg atttttttct aaatactata
     7921 tatagtggcg tctatcctat ataccactaa aaaactacaa aatctagtct tttattttaa
     7981 aaaaacacgt gtagaatgaa gatagacttt tgagttaggg agtgtttttt tatgaaactt
     8041 actgtttttt gcggttcccg ttttggtaat aaagaaagtt acaaatttat tgcgcaaacg
     8101 ttaggaaaat acatggcaca agaagaaatc gaactggttt acggtggttc atactccggg
     8161 attatgggag tgattagtaa aacagtactg gaaaataacg gaaaagtcac aggtatctat
     8221 ccgaatgggc tttttgaaac tgaattgcct ggaaaagatg ttactacatt tattcctaca
     8281 gaaacgatcg atgaaagaaa ggtattatta tttgaaaagg gagatgctgt cctcgttttc
     8341 cctggaggtc taggtacatt ggaggaattc tcacaacttc tttcttggat agcaatcggt
     8401 ctgacgcccg acaagccaat cggaatattg aatattggcg gttattacag cggcttgcaa
     8461 caattattag aaacttttgc aaaagaggat tttatggata aaaaatggtt ggatcaagta
     8521 atcttttcca acaatccatt ggaactggtc aatgaattac gagcagtatc tattaaaaat
     8581 cagttattat tgaaggaggc aaaataatga gtcaaatata tttagcagga ccattttttt
     8641 cagaggagca aatcgatcga gtaagtcgta tcgaaaaagc gttggaggaa aacaaaacag
     8701 tcacaagttt ctactctcca cgtcatcacc aagaaagcaa ttatgaacta ttcagcgctg
     8761 gatgggcgca ggaagtatac gaaaaagata tggaagaact gacaaatgca gaatttgttg
     8821 ttgctatcct tgactttgag catcaaacaa tcgatccagg aacggcttac gaattaggtg
     8881 tggcaactat gttgaaaaaa ccgatgatca ttgtacaaga agagacggta cctacgaact
     8941 tgatgattac acaaagtctc catacgtatt taaaatcaga ccaagctgta cgtgaatatg
     9001 attttgaaac attgcctgtt gaaacttacg taggcgagta cttatagaag aggaagggtt
     9061 gtatgtcagt gattttgatt tcaggaacga ttggtgcagg taagagcagt ttgacggata
     9121 tgcttgcaaa agagattgat tctaaaccat tttatgaaaa tgtcgaagat aacgaagtat
     9181 tgccgctctt ttattcaaat cctgagcagt acgctttttt attgcaaatc ttctttttga
     9241 acaagcgatt tttagcaatg aaaaatgctt tagtgaacga tgacaatgtg ctagaccgat
     9301 ccatctatga agatagcctg ttgttccatc taaacgctga tttaggtcgg gtaacggata
     9361 tcgaagtaca gcagtacgat agcttattag atacgatgct aaacgagtta gatgatgttg
     9421 ctccaaaaaa acggcctgat ttgatggtcc atatcaaagt ttctttggac acgatgctag
     9481 aacgtatcaa aaaacgtggg cgtgactatg aacagctaga aagcgatgag acgttatata
     9541 cctattatga aacgttgaac acccgctata atcaatggta tgaagatttt gatgtatgtc
     9601 ctaaaataca agtggatggg gataaattcg attttgtaga aaatccagaa gatgcaaaaa
     9661 aggtgatccg tcagatccaa gaaaaattag ctgaattgga aggaaagcct gctggaaaat
     9721 attttacgga taaccagcat gcaaaaggtc aagaattagg ctaaaaataa aaaaccaaac
     9781 gacgggaaag aaaaatt
//
LOCUS       DASJST010000104         9486 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJST010000104 DASJST010000000
VERSION     DASJST010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.105"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..74
                     /locus_tag="U4B05_002101"
     tRNA            2..74
                     /locus_tag="U4B05_002101"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="U4B05_002102"
     tRNA            78..150
                     /locus_tag="U4B05_002102"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="U4B05_002103"
     tRNA            155..225
                     /locus_tag="U4B05_002103"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="U4B05_002104"
     tRNA            238..311
                     /locus_tag="U4B05_002104"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="U4B05_002105"
     tRNA            323..394
                     /locus_tag="U4B05_002105"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="U4B05_002106"
     CDS             660..1853
                     /locus_tag="U4B05_002106"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN2051224.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="U4B05_002107"
     CDS             1983..3455
                     /locus_tag="U4B05_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2051225.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="U4B05_002108"
     CDS             3538..4326
                     /locus_tag="U4B05_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN2051226.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="U4B05_002109"
     CDS             complement(4393..5061)
                     /locus_tag="U4B05_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN2051227.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="U4B05_002110"
     CDS             5176..6888
                     /locus_tag="U4B05_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051228.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="U4B05_002111"
     CDS             6891..8663
                     /locus_tag="U4B05_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051229.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9486)
                     /locus_tag="U4B05_002112"
     CDS             complement(8745..>9486)
                     /locus_tag="U4B05_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2051230.1"
                     /translation="YNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEI
                     KKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNK
                     ITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISK
                     NGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHED
                     SKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2852 a         1585 c         2048 g         3001 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cgggattata ttttaatcca
     9421 tatcgacggt tggcgaattt aaaaatcatt cctgtaaaag tttttaaatg tattacataa
     9481 ttatat
//
LOCUS       DASJST010000105         9483 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJST010000105 DASJST010000000
VERSION     DASJST010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.106"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            14..916
                     /gene="gnd"
                     /locus_tag="U4B05_002113"
     CDS             14..916
                     /gene="gnd"
                     /locus_tag="U4B05_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN2051231.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            918..1919
                     /locus_tag="U4B05_002114"
     CDS             918..1919
                     /locus_tag="U4B05_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN2051232.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1916..2200
                     /locus_tag="U4B05_002115"
     CDS             1916..2200
                     /locus_tag="U4B05_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN2051233.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2233..3030
                     /locus_tag="U4B05_002116"
     CDS             2233..3030
                     /locus_tag="U4B05_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2051234.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3044..3877
                     /locus_tag="U4B05_002117"
     CDS             3044..3877
                     /locus_tag="U4B05_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2051235.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3895..4752
                     /locus_tag="U4B05_002118"
     CDS             3895..4752
                     /locus_tag="U4B05_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2051236.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4774..5283
                     /locus_tag="U4B05_002119"
     CDS             4774..5283
                     /locus_tag="U4B05_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2051237.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5320..5721
                     /locus_tag="U4B05_002120"
     CDS             5320..5721
                     /locus_tag="U4B05_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051238.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5833..6186
                     /locus_tag="U4B05_002121"
     CDS             5833..6186
                     /locus_tag="U4B05_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051239.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6220..6663
                     /locus_tag="U4B05_002122"
     CDS             6220..6663
                     /locus_tag="U4B05_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2051240.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6680..7765
                     /gene="uxuA"
                     /locus_tag="U4B05_002123"
     CDS             6680..7765
                     /gene="uxuA"
                     /locus_tag="U4B05_002123"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2051241.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7758..8471
                     /locus_tag="U4B05_002124"
     CDS             7758..8471
                     /locus_tag="U4B05_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2051242.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8678..8827)
                     /locus_tag="U4B05_002125"
     CDS             complement(8678..8827)
                     /locus_tag="U4B05_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051243.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9223..9423
                     /locus_tag="U4B05_002126"
     CDS             9223..9423
                     /locus_tag="U4B05_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2051244.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3182 a         1495 c         2107 g         2699 t
ORIGIN      
        1 tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc
       61 tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt
      121 tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac
      181 ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat
      241 gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc
      301 ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt
      361 ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg
      421 aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag
      481 aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg
      541 aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga
      601 gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg
      661 ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg
      721 gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc
      781 tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga
      841 tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt
      901 tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg
      961 gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta
     1021 ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca
     1081 ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc
     1141 tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac
     1201 accagacaat cgtatctatc atacctgctt taattgaacg aaatgctgta gcagaaaata
     1261 atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg
     1321 acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg
     1381 gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc
     1441 gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg
     1501 caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga
     1561 atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa
     1621 atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg
     1681 gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac
     1741 gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct
     1801 acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc gaacagatca
     1861 gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat
     1921 gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga
     1981 aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt
     2041 cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga
     2101 aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg
     2161 aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa
     2221 ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct
     2281 actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga
     2341 caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc
     2401 caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca
     2461 aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg
     2521 aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa
     2581 gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct ttcagagatt
     2641 atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag aaatgcggca
     2701 gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat
     2761 ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa
     2821 atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg
     2881 ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa
     2941 gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg
     3001 gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt
     3061 agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg
     3121 aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat
     3181 catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg
     3241 ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt
     3301 accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt
     3361 cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa
     3421 atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct
     3481 ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt
     3541 aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat
     3601 tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat
     3661 cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa
     3721 atctttagat attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt
     3781 tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc
     3841 ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct
     3901 gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa
     3961 gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg
     4021 ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct
     4081 ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc
     4141 ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa
     4201 gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg
     4261 gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca
     4321 atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc
     4381 tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc
     4441 cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga aatgctatca
     4501 gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca
     4561 ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg
     4621 ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga
     4681 ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc
     4741 ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg
     4801 gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat
     4861 tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa
     4921 aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc
     4981 tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa
     5041 acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt
     5101 gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca
     5161 gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa
     5221 gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca
     5281 taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt
     5341 cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa
     5401 cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc
     5461 attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa
     5521 gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc
     5581 atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt
     5641 ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc tcatcacgta
     5701 ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag ctactttttc
     5761 ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca
     5821 tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg
     5881 tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa
     5941 aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct acagttttcg
     6001 ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa
     6061 aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa
     6121 acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt
     6181 cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt
     6241 acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg
     6301 agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag
     6361 gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta
     6421 attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag
     6481 aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat
     6541 catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa
     6601 gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt
     6661 tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc
     6721 gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct
     6781 gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt
     6841 tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa
     6901 ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt
     6961 gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa
     7021 aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat
     7081 cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt
     7141 caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta
     7201 tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat
     7261 tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga
     7321 actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc
     7381 agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga
     7441 aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag
     7501 aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg
     7561 ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat
     7621 acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta
     7681 tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa
     7741 accactggga gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt
     7801 tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt
     7861 atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag
     7921 aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca
     7981 atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag
     8041 aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata
     8101 cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa
     8161 tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt
     8221 gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga
     8281 tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt
     8341 tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata
     8401 aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt
     8461 tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg
     8521 agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga
     8581 acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg cgtttaaaga
     8641 gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt tttaattctt
     8701 gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa
     8761 cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga
     8821 atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa
     8881 cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa
     8941 agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt
     9001 tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc
     9061 attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg
     9121 taatttgagc gatatatcta catagataaa actccttttg cctatcaaat atttattgca
     9181 acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta
     9241 aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta
     9301 tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca
     9361 gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct
     9421 taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa
     9481 aat
//
LOCUS       DASJST010000106         9432 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJST010000106 DASJST010000000
VERSION     DASJST010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.107"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..680
                     /locus_tag="U4B05_002127"
     CDS             <1..680
                     /locus_tag="U4B05_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2051245.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="U4B05_002128"
     CDS             800..1000
                     /locus_tag="U4B05_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2051246.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="U4B05_002129"
     CDS             1076..1273
                     /locus_tag="U4B05_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051247.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="U4B05_002130"
     CDS             complement(1570..3117)
                     /locus_tag="U4B05_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEN2051248.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="U4B05_002131"
     CDS             complement(3201..4004)
                     /locus_tag="U4B05_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2051249.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="U4B05_002132"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="U4B05_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN2051250.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="U4B05_002133"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="U4B05_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN2051251.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLVALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="U4B05_002134"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="U4B05_002134"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEN2051252.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="U4B05_002135"
     CDS             complement(6389..7318)
                     /locus_tag="U4B05_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2051253.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="U4B05_002136"
     CDS             7513..8196
                     /locus_tag="U4B05_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN2051254.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="U4B05_002137"
     CDS             8357..9400
                     /locus_tag="U4B05_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2051255.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2894 a         1933 c         1868 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcgact aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DASJST010000107         9241 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJST010000107 DASJST010000000
VERSION     DASJST010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.108"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..656
                     /locus_tag="U4B05_002138"
     CDS             33..656
                     /locus_tag="U4B05_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051256.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="U4B05_002139"
     CDS             1106..2155
                     /locus_tag="U4B05_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2051257.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="U4B05_002140"
     CDS             2152..3033
                     /locus_tag="U4B05_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2051258.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="U4B05_002141"
     CDS             3036..3806
                     /locus_tag="U4B05_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2051259.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="U4B05_002142"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="U4B05_002142"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN2051260.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="U4B05_002143"
     CDS             5117..6280
                     /locus_tag="U4B05_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2051261.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="U4B05_002144"
     CDS             6397..6807
                     /locus_tag="U4B05_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2051262.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="U4B05_002145"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="U4B05_002145"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2051263.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         3208 a         1576 c         1999 g         2458 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 c
//
LOCUS       DASJST010000108         9127 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJST010000108 DASJST010000000
VERSION     DASJST010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.109"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            143..1402
                     /gene="lysA"
                     /locus_tag="U4B05_002146"
     CDS             143..1402
                     /gene="lysA"
                     /locus_tag="U4B05_002146"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN2051264.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1389..2189
                     /gene="dapF"
                     /locus_tag="U4B05_002147"
     CDS             1389..2189
                     /gene="dapF"
                     /locus_tag="U4B05_002147"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN2051265.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2192..3073
                     /locus_tag="U4B05_002148"
     CDS             2192..3073
                     /locus_tag="U4B05_002148"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN2051266.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3070..3786
                     /locus_tag="U4B05_002149"
     CDS             3070..3786
                     /locus_tag="U4B05_002149"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN2051267.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3792..4487
                     /gene="dapD"
                     /locus_tag="U4B05_002150"
     CDS             3792..4487
                     /gene="dapD"
                     /locus_tag="U4B05_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN2051268.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4503..5504
                     /locus_tag="U4B05_002151"
     CDS             4503..5504
                     /locus_tag="U4B05_002151"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN2051269.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5501..6862
                     /locus_tag="U4B05_002152"
     CDS             5501..6862
                     /locus_tag="U4B05_002152"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN2051270.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6822..7973
                     /locus_tag="U4B05_002153"
     CDS             6822..7973
                     /locus_tag="U4B05_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN2051271.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7948..9117
                     /locus_tag="U4B05_002154"
     CDS             7948..9117
                     /locus_tag="U4B05_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2051272.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
BASE COUNT         2998 a         1605 c         2065 g         2459 t
ORIGIN      
        1 ttattcttat ttttaaaaaa atatttcgaa atatactaga caattcagaa aattttgttt
       61 aatattattc caataaaatt caaatcttta tcaaatgaaa ttccaataaa aacggaaaaa
      121 aagtgaaaag aggaatgaaa aaatgacaga attgataatt ggcggagtca cagcaagcac
      181 attggcagaa aagtataaga cacctctgta tgtgtatgat gaagaaaaga tgagacaaac
      241 gatgctgtca ttcaagcgag gctttgtatc gaaacgtttt gatacaaaag tgctttacgc
      301 ctctaaagca ttccaaacga ttgagatgct gcatttagtt cgtgaatatg gattcgggct
      361 agatgttgtc agtggtgggg aaatctatac cgctttgcaa gctgattttc ctagagatac
      421 tatctacttc catggtaaca acaagacgcc agaggaactg atctatgcac ttgaaaatga
      481 tttgttgcat attgttgccg ataatttgat ggaagttgaa ttattagcag aattaagcca
      541 acgctattta aaaaaaatct gtatcatgct tcggttgaat gtcggagtag aagcacatac
      601 tcatgaatat atcgtcactg cgcatattga ttcaaaattc ggcatgagct atgaaagtaa
      661 agagtgccaa caagcccttc agttgataaa agaaagcagc tatctagagt tagaaggatt
      721 tcatgctcat atcgggtcac aaatttttga tgttactgct tggttagctg aaatcgacaa
      781 attgacgaat tacttaaccg attttgacca acctctttcc ttgaatcttg gcggagggtt
      841 tgggattcgt tatacacaaa atgatcagcc gatcccagtc gaagaggcac taaaaaaact
      901 gattgaatat acggaagaag ctttggaaaa acgaaaactg accattcgtc aattactgat
      961 cgaaccgggt agaagtattg ttggagaagc tggtacaaca ctatacacga ttggtttcat
     1021 caaaaagaca ccacataaaa aatattactt tgtagatggc gggatgacag ataatatccg
     1081 tccagctctg tatcaggcag aatacgattg tgatttagca gcaaaactgt cggaagaaaa
     1141 aacagagaaa gtgacgattg caggaaaaat gtgtgaatca ggcgatgtgg taattaaaga
     1201 agcttatttg ccgaaagcag aatcgggtga tcttttagct gtttattcca cgggagctta
     1261 tggatactcg atgagcagca actacaatcg agcgacacga ccagcagttg tttttgtcaa
     1321 taatggccaa tcaagactga ttgttcgcag acaagcattt tctgatctac taagggggga
     1381 agtttcctat gatccttcat aaatataatg gctgcggaaa tgattttatc ttattagatt
     1441 acgcagaaga cacagattat agtcaattag ccgcttcttt atgcacaaaa gaaaaatatg
     1501 atatagacgg attgatcgcc gtaaaaatcg atccgttgga aatgatctat tacaacaaag
     1561 atggcagccg cgcgccgatg tgcggaaatg gcattcgctg ttttgctcga tacgtaaatg
     1621 atcagaacat gatgaatgaa caggaattca acgtagagac attagcaggc acaagaaaaa
     1681 ttacggtgtt ggatccctca cctttttatt gcagcgtaga tatgggagtg ccagatgatt
     1741 ctccggaaaa aatcaaagct aatcaaactt atccaataaa aaaccaggaa atagagatca
     1801 atggtgaaaa agtaacgatc actagtttat ttttaggaac gattcatacc gttgtttttg
     1861 ttgaaaatgc gcgtgaggaa gtctggagaa aaagaggtga actgatctgt catcatcctt
     1921 tgttcaaaga aaaaaccaat gtcaactttg tagaagtagt caatgaacaa gaattgatcg
     1981 tccgtaccta tgaaagagga gtaggttgga cacttgcttg cggtacaggt tgctgtgcct
     2041 cttatgtggt tgctaaggaa actgggaaaa tttcagcaga tcaggtagtt gttcacttgg
     2101 aacaaggaga cttgatgatc tctggtgagg atgcgataca aatggctggc ccagcagttc
     2161 atgaatggac aagaatattg gaggaatgaa gatgataaaa ggttcgatcg tggcgctgat
     2221 cacgccgatg aatgaagagg gatcagtaga ttatgctggt ttggaaaaat tgatccaatt
     2281 ccatttagat gaacaaacag atggattatt agttttaggt acaacaggag aatcttctac
     2341 gctcacacaa tctgaggagg agcagatcct tcaactgaca gtaaagaaag tggctggccg
     2401 tgtgccggtg attgcaggag ctggaacgaa taatacgaaa gaaacgattg aaaaagcgaa
     2461 gcattttgct tctttggggg cagacgcact tttggtcatc accccttact acaacaaaac
     2521 aagtgatgca ggactagctg ctcattttac agcaattgca gaagcctcac cgattccgct
     2581 tattttgtat aatgttcctt ctagaacagg catgtcgatt ccaatccatg tattagtaaa
     2641 tttagcagaa catcctaata tcattggttt gaaagaagcc tcaggagata tggcttatgt
     2701 gatggatgcc gctcggttga ttggagaaga attcttcctt tacagtggaa atgatgattt
     2761 gatcctacca gtaatgagtg taggaggaag cggtgtaata agtgtatggg cgaatatcca
     2821 acctaaaata gtccatgaat tagtaaaaga tacgcaggac ggaagatggc agcaagcaaa
     2881 agaaaagcaa ttaaatgcac ttgaactgat ccatgccttg ttcagtgaga ccaatcctat
     2941 tcctgtcaaa gcagccatgt cattattaga cttaccttcc ggacctctac gcctgccttt
     3001 agtttctctt tcaaaagaga aaaagaaaca attagctcag cttttgttaa aagaaaggac
     3061 ggttgaaaaa tgaaaatcgg actgattggc agtggacaga tgggaaatag agtcgaagaa
     3121 gttgcacaag aaagaggaga tacggtcctt cttttacgga cggcacctaa agaagcaact
     3181 acgcttgctt ttacggaact gccagaactg cttattgatt tttctcatcc agataattta
     3241 gagaaaatcc tttcgtatag cttatcatat caacttccgt tagtcattgg cacgacaggt
     3301 tatacacaat cacagttcca agaaataaaa gaacatgcca aacatgtacc tgtgatgtat
     3361 agtgctaatt tttcgttcgg catcatggtg atgaatcaac taatcaagca agcagctgca
     3421 atgttgcaag gctggcagat cgaactgatt gaaaaacatc atagtcgtaa aaaagatgca
     3481 ccatctggta ctgcaaatat gctgcttcaa acaatccaag aagtgcaaaa gctgcagccc
     3541 gtatacaact ggcaagggaa aaaaagagaa gaaaaccaaa ttggtgtaca cagtattcgt
     3601 gcaggttctt tacctggtga acatgatgta ttgtttgctg ctactgatga aatatttacg
     3661 atcaaacatg aatctttttc taatcgaatc tttgctgcag gcgcagtaga agcagcggac
     3721 tggttgaaag atcagtcacc aggttattac aaattagaag atttattaat ggacaaaggg
     3781 gtgtaaaaaa cgtggaagct caagaaatca tccgctacat tcaaacggca tctaaaaaga
     3841 cgcctgtgaa agtctatctg aatgtaaccg agccaatcga atttaaagaa agtaaagttt
     3901 ttggtgaagg aagcagcttc gttgtttttg gtgactatga gacgattgct ccggttttag
     3961 aagctgaatc agaaaaaatc attgaaatcg aaatcgaaac tgctgcaaga tacagcgcag
     4021 ttcctttgct agatattaaa aaaattaatg ctcgtatcga accaggtgct attatcagag
     4081 atcaagtgtc aatcggcaac aatgccgtga tcatgatggg agcgattatc aatattggtg
     4141 cagtaatcgg tgaaaacacg atgattgata tgggtgctgt cttaggtgga cgagcgacag
     4201 tgggcaaaaa ctgccatatt ggtgcaggag ctgtcttagc aggtgtgatc gaaccagcat
     4261 cggccaaacc ggttatcgta gaagacggcg tactggtagg tgcgaatgca gtgattgttg
     4321 aaggtgtcca tatcggaaaa gatgcagtcg tggcagcagg agcagtggtt ttagaagatg
     4381 tggcagcaga aactgttgtc ggtggaatac ctgcacgtgt attaaaaata agtgatgatc
     4441 agacaaaaga aaatacagca ttgattgctg cgttacgcga attatagagg agatggagaa
     4501 aaatgcgcga aatagatacc tgtattgtgg gggcaaccgg attagtcggc caaacgatgc
     4561 tcaaagtgtt agaagaatat gattttccag taggacgatt gaaattatta gcttcaaaac
     4621 gttcagccgg taaagtttgt gtattcaaga aaaagaaaca cactgtacag gaattgacag
     4681 aaacttcatt tgaaggatat gatttagcgc tattttctgc aggtgcttct gtatccaaag
     4741 agtttgcgcc aatcgcacga agtcagggac tatttgtcat agataattcc tcagcatggc
     4801 gggaagattc ggatattcca ttaatcgttc cagaggtcaa tctggaagac tatacattga
     4861 atcggctgat tgcaaatcca aattgttcaa ccattcaagc ggtacttccg cttaaagctt
     4921 tacaagaagc ctttggtatc cagcgagtca attattcgac ctatcaagca gtttctggtt
     4981 ccgggcaaaa aggcattgaa gatcttcatc gtacgcaaaa tgacgaggaa ccagttttgt
     5041 atccttataa tattagccaa acggtgattc cggaaatcga tcagcctttg gaaaatgggt
     5101 atacaaaaga agaacagaaa atgataaatg agacccgtaa gattctgcat cagcctgctt
     5161 tgccagtatc agcaacctgc gtacgtgtgc caatcgaaaa tggacatgga gtctctgtgg
     5221 cagttcaact gcaaaaagag tttacgacgg aagaaattag agagtgtctc agccagtttc
     5281 cagggattca attagaagat gatctagcag cacataaata tccgacgagc attcttgcta
     5341 gaggaacaga tatggtttat gttgggagga tcagaaaaga cacttcttta gagaacggat
     5401 tgttgttcta tacgactgcc gataacatac gcaaaggagc cgctgcgaat gccgtacaga
     5461 ttgctgcagc tttaatgcag aaaatagcgg aggtggcaag atgagtaata cgctgaaagt
     5521 agcgaaattc ggcggaacat cgatgtctca tgaagagcaa ttcaaaaaag tccgtacaat
     5581 tgttctttca gatcccaatc gccaagtgat cgttgtttct gctttaggca aacgtacgtc
     5641 agatgatgac aaagtaacag acttacttta tttgatttat gcttaccttc agcatcatgt
     5701 tgattggaca cccctttgga aaaggatttg tgatcgtttc attcaagtaa gagatgttct
     5761 tgccctgcag ttacctattg aagatgaact agcacgcata cagaaacaac tggaagacga
     5821 aaagataact gaagattatt tagtaagtag aggtgaatat ctgactgctc aactgatggc
     5881 tgagtatctt ggttaccagt ttattgacgc aaaagacctg attttctttg actatgatgg
     5941 gaaaatcgat caggaaaaaa cagcacagga aatcacaaaa gcatttaaaa gatatccgaa
     6001 aatggttgtt ccagggtttt acggggcgaa ccctgcagga aaagaaaaat tattgggccg
     6061 tgggggaagc gacattaccg gcgcgatact ggcgaagatg ttgcaagcgg aaagttatga
     6121 gaattggacg gatgtttcag gaatcatgat ggctgatccg cgaatcatcg atcatcctaa
     6181 aatcatcgag gaaatctctt ttagagaact gagagagatg gcttatatgg gggctaacgt
     6241 tcttcatgaa gcagctgttt tcccagttca agaggctgat atccccatcc aaataaaaaa
     6301 taccaatgat cctttcgctc aagggacaaa aatatcgaat cacgaaatag aaaaggaaaa
     6361 tggactgaca ggaatagctg gacgcaagga ttttctttcc atcactttat tcaaacgtca
     6421 tatgtccgat gaaatcgggt ttatctggaa agcaatgagc atttttgcta aacatgagat
     6481 atcaatcgaa catattcctt cagggatcga caatatcgga gtgatcgtct cagcagaagc
     6541 gattgccgat aagttatttc tcatcacgaa agaattaaaa gaaacacttg gcgtggaaga
     6601 aatcgtcgtg atcgaagact tagctttgat ctctgtcgtt gggggtccgc ataaagaatt
     6661 gattggatta tctgggaaag tgttatcgat attgaacaaa ttagaaattc gcacatcgat
     6721 cttatcacaa ggagcacagg agttgaatct gatcatcggt gtaccgaata atcaatatga
     6781 aacagtagtg aagggtatct atgaagggat ggtgaatacg gatgctgaaa gaatatcgaa
     6841 tggcactcca tcaaattcct gaaatcgggt ttcatgaatt caagacaaaa aaatatttat
     6901 atgaacaagt aaaaaacagc gaggccgtta tccatgaaat cggcgaaaca ggactactcc
     6961 tttattttga taatcagcaa gaagaaacga ttgcttttcg aacagatatc gatgcactgc
     7021 cagtcaaaga agaaacgaat ctctcttttg cttctaagca tgaaggttat atgcatgcat
     7081 gtggtcatga cggacatatg gcgatgcttt tagggttgac tgactttata actaaacaca
     7141 ttgaggatct tatttataat gtagtactga tctatcagcc atcagaagaa atttccggtg
     7201 gcgcacaaag cgttattcag tcaggattgt tggataaata tgaagtacaa gcaatatttg
     7261 gctttcatct atggcctgga ctagctaagg gagaagtttt ctctcgacca ggacctttga
     7321 tggcacaaag cagtgaaaca gacattatca ttaaaggcca gtctgcacat attgcttcta
     7381 gtgaaaaagg aatcgatagt ctggaggcag ctgtacgatt tttgaagaat gtttatgatt
     7441 ttgaccggca attaccggca gagtggaatc atctaatgaa atttggccaa atccaaggtg
     7501 gaacgatccg aaatgtttta gctggagaag ttattgtatc cggaagtatc cgttcttaca
     7561 gttcagatat gcaaaattat ttgaagaggg gattagacca tttagcagaa cagtttcact
     7621 ctacagctac tatttctttt cgctataatg acggctatcc agctgtaatc aaccatagag
     7681 gcttatatgc agcactgtta gattcaaaca aactacatga attagatgct ccagttcttc
     7741 aagccgaaga ttttggcata tatacgcaac aatatccatg tgtatttttc tttttaggtg
     7801 tcggtgatac ccctgcttta catgatccga cgttcgattt tgatatggat gttttagaaa
     7861 atggtgtaga atggtacaaa acgattcttt ttacagaaaa tcttcttgta aaaatcgata
     7921 gcagttcaaa agaggagcga gtaaataatg atgaatttat ccaatcgatt tagtcctagg
     7981 ctttctagaa tagaagtatc aaaaatacga caattcgatc agcaaatatc gtcgatacca
     8041 gatgtgatca aactcaccct gggagaaccg gattttccaa ctcctgaaca tgtcaagcaa
     8101 gcaggaatag cagcgattga agaagacttt tctcactata ccggaatgag aggattagag
     8161 gaactgcggg aagcggcttg tatatttcag caacagcgtt atggattaac ttatgatccg
     8221 caaacggaag ttttgacgac agttggtgca acggaagcga ttgcctctgc gttgttatct
     8281 gttttggaag aaggagacaa ggttcttatt ccagcgcctg cttattcagg atatcagcca
     8341 ttagtggaac tagctggtgc tgaattgatc ccgatagata cttctgacac agggtttgtc
     8401 tgtcagccgg aacaatttga acgagcattt gaacaatatg gatcagctgt caaagcagtc
     8461 attttaaact atcctaacaa tcctactggg acgacattgt ctgcaaaaca attgaaagca
     8521 atcgctgagg ttttaaaaaa atatccggta tttgttataa gtgatgaagt atatgctgaa
     8581 ttgacttata gtggaacgca tatgtccata gctacttatc ttccagaaca aacaatcgtg
     8641 ataagcgggt tatccaagtc acatgcgatg actggttggc gagtgggctt tatttttgct
     8701 caaaaagccc ttattgacga actaattaaa gtccatcaat atctggtgac tgccgctaca
     8761 acgatgtcgc aaaaagctgc ggctgaagcg ctaataaatg gggtgaaaga tagcgagaat
     8821 atgaaagagc aatatcaaac acgaagagac tatctggtcc agcaattggc gcctttagga
     8881 tttgaagtga cccagccaaa cggtgcattt tacttgtttt gcaaactccc agctgccatt
     8941 caaactgact cttggcagtt ttgtgtagaa ttagcggaac aggcaaaagt tgcgtgtatc
     9001 ccaggaattg catttggccc agaaggagaa ggttatatcc ggatttctta tgctagcagc
     9061 atggaaaaat tacatgaggc ctgcaaacgg atcaaagact ttttaacaga acagtgattc
     9121 atagatt
//
LOCUS       DASJST010000109         9043 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJST010000109 DASJST010000000
VERSION     DASJST010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.110"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            88..477
                     /locus_tag="U4B05_002155"
     CDS             88..477
                     /locus_tag="U4B05_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEN2051273.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            653..1396
                     /locus_tag="U4B05_002156"
     CDS             653..1396
                     /locus_tag="U4B05_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051274.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1389..2969
                     /locus_tag="U4B05_002157"
     CDS             1389..2969
                     /locus_tag="U4B05_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN2051275.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3220..3699)
                     /locus_tag="U4B05_002158"
     CDS             complement(3220..3699)
                     /locus_tag="U4B05_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2051276.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3715..4467)
                     /locus_tag="U4B05_002159"
     CDS             complement(3715..4467)
                     /locus_tag="U4B05_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2051277.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4584..4654
                     /locus_tag="U4B05_002160"
     tRNA            4584..4654
                     /locus_tag="U4B05_002160"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4616..4618,aa:Gly,seq:ccc)
     gene            5015..5740
                     /locus_tag="U4B05_002161"
     CDS             5015..5740
                     /locus_tag="U4B05_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN2051278.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5995..6261
                     /locus_tag="U4B05_002162"
     CDS             5995..6261
                     /locus_tag="U4B05_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN2051279.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            6258..6689
                     /gene="ruvX"
                     /locus_tag="U4B05_002163"
     CDS             6258..6689
                     /gene="ruvX"
                     /locus_tag="U4B05_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN2051280.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6714..7019
                     /locus_tag="U4B05_002164"
     CDS             6714..7019
                     /locus_tag="U4B05_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN2051281.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(7100..8245)
                     /locus_tag="U4B05_002165"
     CDS             complement(7100..8245)
                     /locus_tag="U4B05_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2051282.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(8306..8953)
                     /locus_tag="U4B05_002166"
     CDS             complement(8306..8953)
                     /locus_tag="U4B05_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051283.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
BASE COUNT         2892 a         1549 c         1732 g         2870 t
ORIGIN      
        1 ggcttaaccg tctctactaa aatgtgtcca agatattgac tcaaatccac ttatgaaaaa
       61 aataaaaaaa gcatccaaag ttacttattg aaagaaaaag aaaactatcc tgttcaacat
      121 gctacgaaaa aaaggtggac aactgtccgt tatggtaata gcgatcacta tatccattac
      181 gatgaaatcc tttatctaat gagcatgcca ggttataaaa ataaaatagg cctagtattg
      241 aaagatgaag agattctcgt aaacggcaca attaaaaaat ataaaaaatc actcgaatat
      301 gagtatttat gtaaagattt aaaagcttat atcattaata ctgaaaacat tacatctatt
      361 agcagagtaa acgaaatagt aacatttgca aatggagaac aactagagat gggaaagaga
      421 agtattgata aactaaagaa ttttatgcaa gaaaagatag aaaatgaatc attttaaaga
      481 aaacaaagac tcatatctta cctgttagaa gagtgttttc ctgtagaaga gaacaaccca
      541 agtagcagcc tagtcgtttc tatccttttt tgttatgcac tctttttgac atttactcca
      601 ccaataacga agggtacaat aaaaagtaat aacaaacaaa aggagtacag aaatgattga
      661 attcatcaat gtaagtaaaa gttacgggaa aaaacaagca ttaaaagact tgtctctctc
      721 gataagacaa ggggaaattt ttggattttt aggacataat ggtgccggaa aatcaactac
      781 gattaaaagt ttagttagta ttattgaacc atcatcggga acgattttag tagatggaat
      841 gaaattgacg gaaaatcgat tgagtatcaa acaaaaaatc ggttatgtgc ctgattcccc
      901 agatattttt ttacaattga cggctggaga atattgggac ttgatttcag ctgcctatga
      961 attagacaca caaaaaaaag aaaagcgttt agcagaattg acagcgcttt ttgatatgta
     1021 tagtcatcaa aatgaaacaa tcgcaagttt ttcacatgga atgcgtcaaa aaacgatttt
     1081 gatcggcacg ttgctgccag atccagatat ttgggtattg gatgaacctt tgcaaggact
     1141 tgatcctcaa gcagcatttg atttgaaaga aatgatgaag gctcatgccg caaaaggaaa
     1201 aacagtcatt ttttctacac atgtattgga taccgcacaa cagttatgcg ataaactggc
     1261 tattttgaag aaaggtgaat tgatttatca gggggcagtt agtgaattat tgaatggctc
     1321 acctgatgaa acgctagaaa agatttattt gaaaatggct ggaaggcaag cgacaggaga
     1381 tggaccgtat gtataagcga caattattag aattaattcg agttaatctt ttatatgtta
     1441 atccacagct cacgaataaa gcaagagaga aaggaaaaaa gggaaagcga ttaactatct
     1501 atcttttgtc tcaaaatctt ttttcagggc tgattttttt actgatttac ggatttgcta
     1561 tgtttgctat gaactttgtt aaattgccag gtttctttac ttattacgtg gcactttttg
     1621 gtatgttagg tttaactcaa agtatatcag tcatttataa tatctttttt gaaggaaatg
     1681 acctgcaagt atttttgcct ctgccttttg gacaagaaca ggttttttca gcaaagattt
     1741 tggtagttgc tttgaccatc attccttttg tttttccgct tctagtcatg tttattttaa
     1801 ccggatggcg agctgatcta gggattttgc ttactatcat tatctcaatc cttttgtttt
     1861 ctctattttt gattattctt ttttgtctgg ctagtttgat tgtttttggt ttggctcgaa
     1921 cagcagtgtt taaaaaacat agaaaaacag tttctagtat cttgcttggc agctcgatgc
     1981 tgatcgcatt tgctggaatc atgtggatga atcatcaaac ggggactatt gaaaatgagt
     2041 taacagatcg gcatccgatt tcgatattat tgccattcta tgatgctgcc agtcagccac
     2101 tttctcaaca aggtttattg ggttggggaa tactagctat cacggctctg ggatttttcc
     2161 ttgctgtaaa acagctgata gtaccaaagc ttacagagca actgactgat tcaaacagtg
     2221 agttcaaaat aaaaagaacc cataaaaaga accaaaatgt gcatcagctt ttatttagtt
     2281 acaatgctca attagtaaaa gaacctaatt tgattatgca agttctttct tcttccttgt
     2341 taactccaat catttttatt cttgcttttg ccctaggagg cgaaataaaa ttaactgatt
     2401 tagataataa gttaatcggt gttgttttcc tggtagggat cgcattggct ttcttaacag
     2461 ttaatcagac ttcttttatc agcaatctta tttctttgga tcaagaaaat tttctgttta
     2521 tccgttcttt gccaatctct atgaaccaat atttaaaaga aaagttcaga ttcggatgga
     2581 tattgcagag ttttttaact ggtgggatcg cattattaag cggactgtct tttcagctgc
     2641 ctttgttttt tatccctagc ttgctatttg gttctttatt cggctgctac ttattgtcac
     2701 tacgttattt cgctagagat tatcggttgc tgctgctgaa ctggaccaat attaaccaat
     2761 tatttaacag aggttcagga agtttgggtt tagtcgcaac tatgatgggt tcattgatca
     2821 tcagtattat tcttatcgtc ttatacggaa tgttggcttt gttttatcca ttttggttag
     2881 tgaatattcc tgtgatcggt ttagttctaa tactttccgc tctatggatc gtttattatc
     2941 aacgaacgtt ttggaaaaaa tttgaataaa gaagaggggc cgtgacaaaa atcatgtcgc
     3001 agcccctttt ttacagatag agggtgttca aaagtcagtt tctcagtatt aagtcaattt
     3061 tatgtaaaaa tgtgagaagc tatttttaca aaactttttt taatactcat aattacttct
     3121 tattgctcct gcggttactt gtcgcaatgt ttgtgagata ttttgtcatc gctcaaggct
     3181 gaacgtctct ttcacaacct tttctttgtt acacttcgtt tatttaaaaa gccgtttttt
     3241 tcgtttcatc atgattgcgt cacgctttac tttgaaatta cggatgttat ggttaaagac
     3301 ttctgataat acaagaccta aagcgattgc tccagcaatc ataattgctt ggaccgcata
     3361 atgagccgct tctcgatagg attcagtaac gagatttcga accgcttgat aagctaaacc
     3421 accagggact agaggaacca tcccgggaat attgaaaatc gttacgggca ttttcaaacg
     3481 tttagagaaa agatcgctga tgaatgctac tcctaaagaa ccaagcaatg aaccaaaagc
     3541 agcagatccg cctatttgaa cacagatcca gtagatcatc cagccaaaag caccagaaag
     3601 accacaacaa atcaatgaac gcctaggtac atttgtaata acggcaaatg ccgcagtcgc
     3661 taaaaaacta aaagaaaact gaataaatat cgtccccata cttgtgttcc tttcctaata
     3721 aaaaagctgg aaaataaaag caatcgcaaa accgatcatc gtagcagtca tcatcgcttc
     3781 tgttccgcga gaaactcctg agacataatg tccagctagc aaatcacgga tagcgttcgt
     3841 aatttgaact ccaggaacta aaggcatcac acatccgata atgattagat cctgattgct
     3901 tcctaagcca aaacgtacgc tgtagatagc ggctatgcca atcaataaag aagataaaaa
     3961 ttccgataaa aatttgattc taaaaaattt caaactatat agataagctg cgtatcccaa
     4021 tcctccaatg actaaagtca caggtaaatc ggtcatcttt ccgccaaata acaccataat
     4081 tgttccactg atcactgccg cgctaacgat ttgcaaccag acaggaaaga aagaacggtc
     4141 tcggtcgatt gcttgcagtt cgcagtaaag ttcttctaat gttagctctg aagcaacata
     4201 tttacgtgaa agatcattta cttttactac tttttctaaa ttaatcgaac ggttggtaat
     4261 ttgttccatt cggatggtcg aggtacgatc aagaccaata aagagaccgg tttgagtgac
     4321 atagctgacc aaacggaaat ttccagaagc taaagcaatt cgactcatcg tatcttctac
     4381 ccgatacatc tccgcattgc tttccatcat gattttccca gcgagtaaac aagtgtccag
     4441 caacagatct aattcttttc ccgtcatagc atcttctcca ataattgatt gattagtttt
     4501 attttactac aaaacatacc agaagcgtaa agaataaaaa aacttgagga aggaataaaa
     4561 gaggcgtata ctgtttttgt attgcgggtg tagtttagtg gtaaaatccc agcttcccaa
     4621 gctgatgtcg cgggttcgat tcccgtcacc cgcttaaaat aggttgtgaa aaagctgctc
     4681 tgcaacaagt aataagaaca gccaagcaaa aacaacttct tatgttttta acttggatgg
     4741 acttattacc gcagatgcaa gcttttgcgc accgtttacc aagcggttgt gaaatcagtg
     4801 tattgtcttg agtatgaaga aaatttttga ataataataa ataccttaca gatagagtgg
     4861 tgactgatta gagacgagga atccgtctct tttttgtatt tttaagccat cttaaaacag
     4921 gttttatttt ttgctgtttc tatttctttc tagtatttat ttatagggga acatatgtta
     4981 agatttaggg agctgttagt aaaggagtaa agaaatgagc aaaaaaacaa ccaaggcgat
     5041 cgtggggatc gtcgctgctg tgattgcagg ggcattcggt gtttccacca cacaaaaaga
     5101 ttcagatatc tttcagcaga ttacaactat ttttgattcc acaccacaag cttctcaagt
     5161 agcggatgtg ccagaatatg atggaaaaca tcaagaaata gaaatcaatg ggaatcagcc
     5221 ggaatttaca aaagaggatc ttagcctttc caaaggaaca tgggaaagct attcaaatat
     5281 tgataaattg aacagagtag gacaagccaa tgctatgctt ggaaaagatt tgttccctac
     5341 tgaaaagaga gagcctttgt atattgaccc gacaggttgg aaacaaaaga aattaagtga
     5401 cgggcaatgg ttatataatc gcagccattt aattggtttc cagctgactg gtcaaaataa
     5461 caacattaaa aacttgatga cagggacacg ttcgttgaat gctccataca tgcttgctca
     5521 cgaaaatgat attgcggcat atatcaaaga aacaaatcat catgtacgct accgtgtcac
     5581 tcctcatttt gaggggaatg aactggtagc caggggagtg caattggaag cagaaagcat
     5641 tgaagacaaa aaaattgaat tcaatgtctt tatctacaat gttcaagatg gctacacgat
     5701 caattatgag acaggacaag caacaaaaac aaaaaattag tagcagctat tcgaattttt
     5761 tatgaatatt atgaaaaaat tgtgtaaaaa catacattca aagaaatata gcgtatactt
     5821 tatgaaaaga gtacttttga taaagctaca gaaaaaagga aaaaaacata caaatagtgt
     5881 ttcttaattt attcggaagt ttggaatatt tgtttgtagt tttccctttc ttcgagtaaa
     5941 ataaggttgt agaaggtaag ctggaattac cttgcgggaa agaggtgtag aatcatgggt
     6001 tttacagatg aaaccgtacg ttttggtttt gacgatagtc gcaaaaaaga agtgagcgaa
     6061 acattagcga ttgtttatcg ggctttggaa gaaaaaggct acaatccaat caatcagatt
     6121 gtcggctact tgctttctgg agacccgtcc tacattcctc gttatcgtga tgctcgaaat
     6181 ttgattcgcc gtcacgaacg cgatgagatc atggaagtaa ttgtcaagga ctacctctca
     6241 aatcatggag tgaatttatg agagtaatgg gcttggatgt cggttctaag acagtaggtg
     6301 ttgctatcag tgaccccttt ggctggacag ctcaaggagt agagatcatt cggatcaatg
     6361 aagcagaaga agagtttggc tttgatcggt tgaaggaatt agtagcacaa tatgaagtag
     6421 accggtttgt agttggatta cctaaaaata tgaacaattc catcggacca agggcagaag
     6481 cttccatgtc atacggagaa aagatcaaag aactattctc cttgccagtt gattatcaag
     6541 atgaacgatt aaccactgtt caggcagaac gtatgctagt agagcaggca aatacttcaa
     6601 gagccaaacg aaaaaaagtc atcgataaat tagcagctgt gatgatctta caaaattatt
     6661 tagacggctc aggtaagcaa ttattttaat aataaaagaa aagaggtaat cttatgacag
     6721 aacataacca ttcccacgac catgaaggac acgaacacat cacattggtg gatgaccaag
     6781 gaaatgaaac attatatgaa atcctactaa cagttgacgg acaagaagaa tttggacgta
     6841 actatgttct tttatatcct gctggtgttt cagaagatga agatgtagaa ttacaagctt
     6901 atgcatacat cgaaaacgaa gatggaacag aaggcgagtt agaacaaatc gaaacagacg
     6961 cagaatggga catgattgaa gaagtattca atacattcat ggcggaagaa gaagaataga
     7021 ctataaagct aggagaaacc ttgatatgat agggtttctc ctttttttat tttttactgt
     7081 atgccatatt gtatgccatc tagtcaatat ttaagtgttg tactaatttt tcaacagtct
     7141 tttctttcgt ttcttttgcc aagtgaccgt aacaatcgat cgtctgacta atttttgtat
     7201 gccgtaatct agtttgaact tctaccatag tagctccagc gtccaagagc attgaagcat
     7261 gagtgtgtct tgctacatgc acaccgagaa atggaaccat agctttttca caaatagtaa
     7321 tcaatcttgc ataaatagcg tggtgtgtga ttagttcttt atagatacca caaaaaatca
     7381 tagtcggatc agttacacca agtgtcagca tatatttccg ctgattaatc tgccattttt
     7441 ttaatataga aattgttctt tcatctagtg gaagaacccc agcactacaa tcagttttag
     7501 gatcactgat tttatagcca tttttggatt ggcttagtgt tttattgatg gtgaccgtat
     7561 tttcttgaaa atcaatgtct gaccaattca atgcaagggc ttcactgatc ctaaccccgc
     7621 tataaaagag aaagcgtagt aacgttttat caaagtcgtt tcgataagtt tcaggtttgc
     7681 tgtttaggta atcaaacaag gtgatcagtt gttctttact gtaaactttg accttttctt
     7741 tatgcttagt ggtggttctt ttaggcatta atacgttgtc tagtggatta gtctcaataa
     7801 tcccaagatt gatagcgtag ctaaaaatac gcttagaagt tgaatgaagt accttgtatt
     7861 ggtcagtagt ttcagcccag ctatttacaa tttgttgggc tttttttacc gtgacttcat
     7921 tcacacgaag atttccatat tttggaagaa tccactttct aaacttgatt tcagttttca
     7981 tgaaggtggc ttctcggaca gtggttttat atgtttctag ccacaagtca taaacttctt
     8041 taaacgtttc gttgttcgct tttttaatac cgtttttatc gtagtccact tgcaagcgtg
     8101 acaaggcaag ctgggcttct ttttttgttt tgtaacctcg tctacgaacc ttatcaggtt
     8161 tgccagtcat tttgttcatt ccaagatagg cttcatagag ccaaaattta ccttttttag
     8221 tttcatattg cttgaatgtt gccataatag ttttccctcc atagtgtagc aagccgtggg
     8281 ggcggttgga ggaggcagtt tattattatt ttgaatagtg tttatttagc tcagtaacaa
     8341 gagctgtgaa ctcttttaga agatcatttt cataaaattc ttgatactct tttttctcat
     8401 ctgtattttt ttctgaatca ttaattaatg agtcatatcg taaaagcgta tttagaagag
     8461 ctgcgagata agcaattggg gtactcccgt caggaaattc aattcgagat cctgttttaa
     8521 gatattttat tgcgtttgtt aaataaatct tctgtgagac agtcaggtta tcttcttcga
     8581 tgttattgat agcattagaa agattagaat caattataga gttatttttt tcaatagttt
     8641 ttttcaattg cttttcaagc acatttcgct taatttcggg catcatatgc atatctctta
     8701 atgtataaga tccgtttaat agataggtag tggatacatc taataccttt gaaagttgtt
     8761 taagcctggt gttattggga agattatagc cattttccca attactaacg gcacctttac
     8821 tggccggtgg ggataattta cctccaagtt gttccattga gagtcccttt tcaattctag
     8881 ccagtctaat tctttgtcct gtctccactg cttcggggta tttttttcta gccacatttt
     8941 caccaccatt catatatttt ctctactata tcacgattta aatatgtgtg aaacataaaa
     9001 gttcaaaaaa gtatgttaaa attcttgcaa atgaaaaggt atg
//
LOCUS       DASJST010000110         8923 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJST010000110 DASJST010000000
VERSION     DASJST010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.111"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..859
                     /gene="purR"
                     /locus_tag="U4B05_002167"
     CDS             41..859
                     /gene="purR"
                     /locus_tag="U4B05_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN2051284.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1362..2735
                     /gene="glmU"
                     /locus_tag="U4B05_002168"
     CDS             1362..2735
                     /gene="glmU"
                     /locus_tag="U4B05_002168"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN2051285.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2829..3800
                     /locus_tag="U4B05_002169"
     CDS             2829..3800
                     /locus_tag="U4B05_002169"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2051286.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3806..3908
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3974..4543
                     /gene="thiT"
                     /locus_tag="U4B05_002170"
     CDS             3974..4543
                     /gene="thiT"
                     /locus_tag="U4B05_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN2051287.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4624..5397)
                     /locus_tag="U4B05_002171"
     CDS             complement(4624..5397)
                     /locus_tag="U4B05_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051288.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5664..7364
                     /locus_tag="U4B05_002172"
     CDS             5664..7364
                     /locus_tag="U4B05_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN2051289.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7334..7552
                     /locus_tag="U4B05_002173"
     CDS             7334..7552
                     /locus_tag="U4B05_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN2051290.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7572..8462
                     /locus_tag="U4B05_002174"
     CDS             7572..8462
                     /locus_tag="U4B05_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN2051291.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8480..8764
                     /locus_tag="U4B05_002175"
     CDS             8480..8764
                     /locus_tag="U4B05_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051292.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3008 a         1529 c         1883 g         2503 t
ORIGIN      
        1 aatgtaaaac gataaaattt cttaaaagga gttggaatag gtgaaagtac gtcgcagtga
       61 acgtctagtt gacatgacac agtatttatt gaatcacccg catgaattga tttccttaac
      121 aagctttgcc gaacgttatg aatctgctaa atcttctatc agtgaagatt tgggaatcat
      181 caagaaaaca tttaaagaac gagggtatgg aaccttggaa acaatcccgg gtgctgcagg
      241 tggtgtattg ttcatccctg aaatttcata tgaagaggcg aaaaaatata ttgaatctct
      301 agcagaacgt ctatcagaac aagaccgtct gcttccagga ggatatgttt atctctctga
      361 tttgttagga gaacctgaac tgctgcgtca agttggaaga atcattgctt ctaaatacct
      421 tggtgaaaaa atcgatgctg taatgactgt agccacaaaa ggcgtaccta ttgcccaagc
      481 tgtgtcctat tatctgaatg cgccattcgt tattgtacgc cgggactcaa aaataacaga
      541 aggatcaaca gtaagtgtca actatgtgtc tggatcttct gaaagaattg aaaagatgga
      601 actgtctaag agaagtttga aacgtggttc acgtgtcctt gtagtggatg atttcatgaa
      661 aggcggcgga acagtcaacg gaatgaagag cttgattgaa gaattcgaat cagaacttgt
      721 tggcatcact gtatttgccg aatcaaaatt caacggtcat cgcgccatcg atgattatac
      781 gtcactgctt tatgtcaaag atgtagatac tcaaacgaaa aatattacgg ttgtgccagg
      841 aaattatttt tcagaataag aggttgtgaa aaagctgctt agtttcgagt agtaagaaaa
      901 attttgtgaa aatggctttc cacatttttt cataaatttg gcttaatacc gagaaactgg
      961 cttttgaaca ccgtttatct agaggttgtg aaatcagcgt cctgtcctga gtattagctc
     1021 aataaacgga aaaattgctc ctcatatttt gccgtttatt gagctaatac cgaaggtcag
     1081 ctgattgaaa aacgtttaat gaagaggttg taagcctacc gtttaactct aagtcacaag
     1141 aaacgatttc aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag
     1201 ttggcaggag agcaccggtt accaaataga taggcccgtt atggtctatc tattttttta
     1261 tccagaaaac gctgggataa aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg
     1321 gataaactga aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa
     1381 ttattttggc ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc
     1441 ccgtttctgg taaacctatg gtagaacata tcattaatcg ggtcagtgaa acgaaaccag
     1501 atgaagtgat tacgatcgtt ggacacggag cagaacaagt taaagcacaa ctaggagaac
     1561 gcagtaaata tgcgttacaa gcagaacaat tgggaacagg acatgctgtt ttgcaagctg
     1621 cttctttctt ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat acaccattat
     1681 tgactactga aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcaa
     1741 cgatcttgac ggcacaagca gaaaacccaa caggatatgg acgaatcatc cgtgaccata
     1801 ttgggatcgt agaaaaaatc gtggaacaaa aagatgcaac accagaagaa gctcttgtac
     1861 aagaaatcaa tacaggaact tactgttttg acaacgaagc tttgttcgat gcgttaagca
     1921 aagttggaac aaacaatgct caaggtgaat actatctaac agatatcatc gaaatcttaa
     1981 aagaagaagg ccatacagtt gctgcttatc aaacggatga ttttgaagaa tcaatgggag
     2041 tcaatgatcg gatcgcgtta gctaaagcaa atgagatcat gagaaagcga attaaccaga
     2101 tgcatatggt caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg
     2161 tagaaatcgg tccagacaca ttgatcgaag caggtgtaca aatccaagga aatacagtca
     2221 ttggttctga ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg attgaggatc
     2281 acgtcgtcat tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg
     2341 gaccttatgc acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact
     2401 ttgtggaagt gaaaaatgct caaatcggca aaggaactaa agtcggtcac ttgacttatg
     2461 ttggtgatgc tacgctggga gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact
     2521 atgatggaaa gaacaaacat catacgactg tcggcgacca cagctttatt ggttctagca
     2581 cgaatatcat cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa
     2641 ttacagacaa tattccagaa tatgcgctgg caattgcgag agcaagacaa gtcaataaag
     2701 aaggatatgc aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta
     2761 tttaaaaaat atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg
     2821 aggtccccat gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca
     2881 atcgcccatt agcagagaaa attgcagcag cagtcggcgt cgagttagga aaatcatctg
     2941 ttactcaatt tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc
     3001 atgtctatgt tattcaatca acgagcagcc cagtcaatga taacttgatg gagttgctga
     3061 tcatgattga cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc atgccttatt
     3121 atggctatgc gcgtcaagac cgtaaagcac gtgcacggga accaatcaca gcgaaattag
     3181 tagcaaacat gattcaaaaa gctggtgcaa caagaatgct gactttggac cttcatgcag
     3241 ttcaaatcca aggtttcttt gatattccag tcgatcattt gatgggtgcg ccacttattg
     3301 ccaattattt cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc
     3361 atggtggtgt cacacgggca cgtaaattag cggaattttt gaaatctccg attgcgatca
     3421 tagacaaacg ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt ggacaagtca
     3481 aaggcaaaac ttgtgtactg atcgatgata tgatcgatac agcaggtacg atcacattgg
     3541 ctgccaatgc tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt acacatccag
     3601 tattatccgg ccctgcttta caaagaatag aagattcagc tatcgagcgt ctagttgtta
     3661 cagactcgat ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg
     3721 gcggactaat gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattgt
     3781 ttgaaacaaa aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt
     3841 gaatctattt ctaaaccctt cgtacctgta tcggttatgc gagcgtagga attgtgaata
     3901 aacagctgtt tgactatttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg
     3961 aggaagtttt tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt
     4021 agcgatggtg ttgtcgttga ttccgatcca gatcggcagc agtttttcta tttcattagg
     4081 gcagattcca ttgactcttt ttgcgttaag aagagggaca aaggcaggga tgctggcagg
     4141 tttcatttgg ggcatcctac attttccgtt gggacaagtt tattatttaa gtgtcgttca
     4201 agtgttgatt gagtacccga ttgcctatac ttttgcaggc gcagcagggc tcatggcttc
     4261 aaatgtccaa aaaggctatg tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt
     4321 tggtgcattg attggcgcac tttccagata tttttggcat tttattgcag gagtggtttt
     4381 ctggggagcg tatgcactat gggggatgaa tccttggctc ttttctttcg taatgaatgg
     4441 tgccagcggg ttgagtacag ctattgtaac gatcatcgtt ttattaggga tccaacaaac
     4501 gactccttcg atttttgtgc cagataaacg acaggagctg taaataaaaa agaagcaaga
     4561 aacttgagac agaatcatgt cctagtttct tgtttccttt ttattttttg ataagaaata
     4621 gatctacttc aatggcagat aagaaagatc ggcatcttct accaattgcc agtgtcgatc
     4681 ggtgacagag tagattaaag tagcgatgat ttcctcttct cgataccggg tgccttttaa
     4741 tttccaaata taatcttcaa tcacatagat tgggatacga tatttaccat cgatcaaacc
     4801 aacttgatca aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa
     4861 ttccacactg attttttcaa caggtgtttc ttttttgaaa aaagattttt ttacattgat
     4921 cttatctgac aagatcgaac ttaatttact agcaaaaatc ctataaaatt catctaaata
     4981 gcgataataa atccgattca gctgatcatt aagatcgaag tatacaaaat tattctgtag
     5041 tttataaaaa aacggcgaat gaagatgtgt tttcatatga ccaaaatata ataattctga
     5101 aatttctaaa ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga tccacttcaa
     5161 ttcatcgaat ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc
     5221 acggattact ttcagccctg tatgggattc atattctcca gcagaagcag atggatcaag
     5281 caagagcaag ttttgaggaa aatgcacgat actgtttgca aagtcagtag cagtcagccc
     5341 ttttgttaaa acagcattac ttgtggtatc aatatgtaca taaactgact cagtcatcat
     5401 gttcctcctc ttatttcctc ttaatctctt ttattttaca acatttgcat taaaaaagct
     5461 tgtttctttt attttaaaat tccattacaa aatcacgttt tcattgaaaa cactctcatt
     5521 ttcaattaat ttttgtgatt tttgtatttt tttatttaaa ggaggtttta tgaattgttt
     5581 tcttatggaa tctacaaagt cgaatacgag actcatgacc tttttatgac gtactggtat
     5641 acttgaagaa aaaggagagc gcgatggaga taaaaaaagt ttacttttca cagaaaatgt
     5701 cgctgtattc gcctatccag caagcaaaac aaccccttcc attttttcaa agtcatcaag
     5761 aaagtagaga cgctttttgg cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga
     5821 aaaaatatac cgtctatttt actattgcta aaagtagagg agatgttatt tttaataatc
     5881 tgttgattga aggtgaaact tttgaatgga aaaaattttt tcggtatatg gaagcttgcg
     5941 ctcgtttttt tattaaagaa aactgttgtt ttttatttca atctcctcta tcagaagaat
     6001 ggttgcgtat ttttcataaa aacggctatc aaggaaccac acaattaaat aaaaagctgg
     6061 tctatcatac agcacttgtt ttaggtggag gaggagcgca cggtgcttat cagattggtg
     6121 tgtggcaggc gctgaaggaa catgatatca actttgagat tatcacaggt acctcggtag
     6181 gtgcgttgaa cggcgcattg attttacaag gagatatgaa aaaagctgtg aatttatgga
     6241 aaaagctgtc gacaagacaa gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc
     6301 gtgaacgctt ctatcgagaa agacgccaaa tgacgaaaac agcactaatt gaaggcgggg
     6361 tttcttctgc tccattggaa aagatggtca aagacaattt ggatttgtcc ttactcaaac
     6421 ataatcccaa aatacgttta tatacggtgt ctactaaact gccagaacta gcggaagtag
     6481 tgactgatat ccaacagaca aaaacagaag aaataccaga ttggatactc gcctctgctt
     6541 cattttatcc cgctatggct tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt
     6601 atcgcaacaa aatacctatt gatattgcga ttaacaaagg agcaacagag gcatttgtcg
     6661 tagatgtgca agggccaggc ccagcgaaaa aaattcgtat gccagacatt tttatccatt
     6721 ggaaatgtca aacattgtgg acactaggca gctttctgct ttttgatagc caaaggaacc
     6781 aattaaattt acaattaggc tatctggaaa tgaaaaaacg attaggctat tactatggga
     6841 actggtatac atttcattct gttaaacctg caagaacata ctggcgagga tttttgtctt
     6901 acttaacaaa agaaattcag ttagaattat cttttttcaa aagcataaaa ttttggcaaa
     6961 aactgcgtaa tttatataag aacagggtcg taccagagac atgcggattg gcgatgctag
     7021 aacttttagc aaaaaaacat tttttactgc ccaatgaaat ctatcaagta gacatgatga
     7081 ttcaaatgat ctgccaacaa gatataaaaa gtatgccgga tgatttattg gcacaaattg
     7141 gtcaattaag tactgaagaa tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg
     7201 agcgcacgaa aactgtttat tttgattatc tgctacaagg aaaacgcaag gatcgtttgc
     7261 agcatgaatt gcttaagcaa ccagttgaca cattactgat cctatattta tattatctca
     7321 aggaggaaca accatggcac aagaattttc atatgaaatc gtagaagaaa tcgctatatt
     7381 gtcagaaaac aacaaggggt ggcgtaaaga actgaaccta gtcagctgga atggtcgccc
     7441 gccaaaattc gatcttcgag attgggcgcc agaccatgaa aaaatgggga aaggcctgac
     7501 attgacgaac gaagagtttg aacaactgca aaaagcaata gaaaatatgt aatgaggaag
     7561 gaccctgtcg aatgaaaagt atgactggtt ttggcaaagc catccgcgaa acaaaagcgt
     7621 atcagttaga tattgaaata aaaagtgtca accagcgttt tctagatgtt caaatacgaa
     7681 gtcctaaaat cctgaacttt gttgaaaacg attttcgtca gcttattaaa cagcagttaa
     7741 gccgcggtcg aatcgaagta tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga
     7801 tcgtcatcaa ctgggaattg attgattcac ttatcactca attgacagac ggaattcaac
     7861 aacgatacgg tagtcaaaat caagtgtcaa ttagtcggat ggcggaacgc ttcgcgctgg
     7921 acgaaaactt cgtaacggta gaagaaaaac cggaagagag catggaagaa ttggcacagt
     7981 tagctctaga tacattgaaa agcgcacttg attctattga ggcgagtcga gaaaaagaag
     8041 gtcaagcttt agctttagtg ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa
     8101 aactcgatag ttttgtagaa atctatgaaa aggaatttca aataaggtat caaaaaaagc
     8161 tggaggaatt tcttggagcg aaagtcgatg agcaacggtt gttgacggag ctagccatct
     8221 tgttggaacg cggagatatt cacgaggaac tggatcgtct ggcgatccat gtacaaaaac
     8281 tagatgaact tatcgaagca caatgtcctg taggtcggga attagatttc ctgatccaag
     8341 aaatgaatcg ggaaatcaac acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc
     8401 aagtcgtcca gtctaagacg atcttagaaa aaatcagaga acaagtacaa aatattgaat
     8461 gacgaaagag gtagagataa tgaaatatta tgacgtaacg ttccacgaat taagtggaaa
     8521 aactgtggtt aaacgggata tcccttctga aaaaaacggc tttgacgtat ggaaagatgc
     8581 ctgtgcagac tataatgaga acgaactgtt tattttgatc aacgacggtg cttacgtgac
     8641 gatgaatcgg aaatttattg ttcgaattga cacagaagag gtagaagatc cgacagaaaa
     8701 agcacgttct cgaaaagatg agatcatggg tgtagtgaat actttgtcca atatgggctt
     8761 ttagcaagga tgctgaacaa agaaatacaa taaataatca gaattggatt tcttcgctta
     8821 gcttctttgc tctaggaata catttaaagg ttgtgacaaa attcgtgtca caatcttttt
     8881 ttattttgaa taagtgatgt tcaaaaacca ttttctcggt att
//
LOCUS       DASJST010000111         8888 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJST010000111 DASJST010000000
VERSION     DASJST010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.112"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(230..604)
                     /locus_tag="U4B05_002176"
     CDS             complement(230..604)
                     /locus_tag="U4B05_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051293.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(626..1003)
                     /locus_tag="U4B05_002177"
     CDS             complement(626..1003)
                     /locus_tag="U4B05_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051294.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(993..1388)
                     /locus_tag="U4B05_002178"
     CDS             complement(993..1388)
                     /locus_tag="U4B05_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN2051295.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(1455..1640)
                     /locus_tag="U4B05_002179"
     CDS             complement(1455..1640)
                     /locus_tag="U4B05_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEN2051296.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(1722..1976)
                     /locus_tag="U4B05_002180"
     CDS             complement(1722..1976)
                     /locus_tag="U4B05_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051297.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(1998..2243)
                     /locus_tag="U4B05_002181"
     CDS             complement(1998..2243)
                     /locus_tag="U4B05_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051298.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(2250..2534)
                     /locus_tag="U4B05_002182"
     CDS             complement(2250..2534)
                     /locus_tag="U4B05_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051299.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(2637..2819)
                     /locus_tag="U4B05_002183"
     CDS             complement(2637..2819)
                     /locus_tag="U4B05_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN2051300.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(3025..3564)
                     /locus_tag="U4B05_002184"
     CDS             complement(3025..3564)
                     /locus_tag="U4B05_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051301.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(3677..3943)
                     /locus_tag="U4B05_002185"
     CDS             complement(3677..3943)
                     /locus_tag="U4B05_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2051302.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(3945..4166)
                     /locus_tag="U4B05_002186"
     CDS             complement(3945..4166)
                     /locus_tag="U4B05_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEN2051303.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4392..5210
                     /locus_tag="U4B05_002187"
     CDS             4392..5210
                     /locus_tag="U4B05_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2051304.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            5214..5510
                     /locus_tag="U4B05_002188"
     CDS             5214..5510
                     /locus_tag="U4B05_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051305.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(5692..6045)
                     /locus_tag="U4B05_002189"
     CDS             complement(5692..6045)
                     /locus_tag="U4B05_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051306.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(6065..7120)
                     /locus_tag="U4B05_002190"
     CDS             complement(6065..7120)
                     /locus_tag="U4B05_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HEN2051307.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            7336..7734
                     /locus_tag="U4B05_002191"
     CDS             7336..7734
                     /locus_tag="U4B05_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051308.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            7737..7910
                     /locus_tag="U4B05_002192"
     CDS             7737..7910
                     /locus_tag="U4B05_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051309.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         2874 a         1402 c         1495 g         3117 t
ORIGIN      
        1 aatatattaa aagctctcag aagccttctg agagctttta aattgctatg tattgtactg
       61 catctgctca aattgatttt cttttcatgc ttaccatgta tctaattttc catcaagtaa
      121 tttttagata tgtattgagt aataaatagc aaatttgctt gatataagct cgaattaggt
      181 gctttgtaat gtgttaggat aaaattatac aagaaaatgt caaaagctct cagaacgctt
      241 ctgagagctt tttatcggta ggcttgttta acgtgatctt gaaggatacc cttttgatgt
      301 ttctctcgat ccctgcatcg ctaattacta agtgttcttc aagtgtgatg taggtatata
      361 aattaagcat caaaatggtg tagataagca aaaaactccc taaaactaag tgttgattat
      421 aaaaatctga cttataaaaa agaactgaga atgttagtgt tgattcccaa aaaagaaaca
      481 aaattttgta tttccaaaga tagtggatca agcgctgtgc tttacccatc tcaaataatt
      541 tataataacc tgctaattga tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt
      601 tcatctttct cttctcctgt ttcaattaat ctaaaatttc agattgagca agcaactcat
      661 tgtggtagtc ggtttgaaat tcaaataaca attttgcctt cttaacgagg tttaaagcgt
      721 tcgttagtga ttcacttaat gcttcaacat ctttaactga ttttgcatgt ttcatttttt
      781 cagtgtgcgt atagcagaat tcaggtattg agttaggggt tgcctgttca ataaacatct
      841 caattcgcaa gttattatcc tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa
      901 cgctaatgcc agttgtatct tttaatagat tattgattaa cgttcgagag aaatagttta
      961 agtgtgtagc tttcataggt tcggtctcct ttttactttt caagcaactc ttcttccagt
     1021 gctttttgaa gtagttgaga gaagtttatg tttttctcct tccctaaaag aactaaccat
     1081 tccggaacac taacattttt tctaattgtt tttgtattta agcttcttcg gaaaagttta
     1141 atgtttagtt ggatgattac taccacttgg ttggctttca aagttaattc ttccactgct
     1201 gatggtttag gaaattctgc atctggtaca ctatatagcg ctaatcctaa agcatcctta
     1261 gcgtttaaat atgcttcttc aagactattt ccaaaggtag atatttccgg taagtcaggg
     1321 aaaacgatgt tataagcttc tccttctttc tcgaagattg ccggataaaa aatatttttt
     1381 gtcttcattt cctcgctcct tttctagtgt aagagaggat agtatgccta ataccaaata
     1441 ggaaggcaag gctgttaaag ccctgcttgc ttccagatat tagcttctgt tcctagtggt
     1501 acatcaccat tgtggatcgg aacaacgacc gttcttttag tcttggaatt ataaaatttt
     1561 gcatgactgc cagtttgtcc agtctttttg aagccgttag atttgagaat ttttacaatt
     1621 tctcttgctg tcttaggcat agctaccctc cctttacaaa atctattata cacattgttg
     1681 tgtatggaat caatagaaaa agaggatgtt ttattgcaaa actaataaaa tgatttattg
     1741 gttttttcag caaattgaat gttattagcg ttgcaaatat tcatgatttc ccaagtatca
     1801 tcagaagtat aggtatctaa gaattcattg acagttacgg ttgattcgta tggttcataa
     1861 gtcaaagcgt tctccaagtc ttttgatgaa ataatcaaga ctttttcaat ttcctcccct
     1921 tcttcatcta tgaaaacata ttctaaagtg atttgttctt catcggtata attcatattg
     1981 tgttctcctt tcttgtacta agaaaaatgg atatttccaa ttgcatccca ctcggcacca
     2041 ttgtggtata ggacgtgacc gagcgttacg cctttgaaat gttcttctaa atacatccaa
     2101 acgcattcaa ggctagtacc tgctttgaga ttgtagaagt ctgcatccaa taccttttca
     2161 gaagttatcg gatcaatgcg agttggtact tcaataaatt cagtccaaat ttctgcaaaa
     2221 gtcttacctt ccaaagtcca cactccctat caattaattc caaaagcaaa tagagaaatc
     2281 aagcgaccgt caatagtcac ttcaacaact gaaaatgccg ttgaaacagt tttaacatct
     2341 acactttcca agttcccgat gaaggaatct ttgtgcttta gaatagcttt ctttagctgt
     2401 ctgttataca cttgccgact gttaaacttt ttgaattctc gatccttgat cagctgttta
     2461 acgatccctg cattaattgt ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa
     2521 gtagctgtta gcatcttgtc actccttcct aaaagttaat tagcttgttt acaaatacat
     2581 tatacacatc ggtgtgtata atgtaaagag aaaaaggtgc taatggatca gctgttctaa
     2641 acaaaagaaa acgataaagt aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct
     2701 agtccggttg ctttgcttgt gtgattttcg gctttcttat agatcaaata ggaaattatc
     2761 agtgatattc cagtaaagaa taatgtacta gctgtcacta aaagaaatag gttaatcata
     2821 cgatacactc cttctgtaat attggtatca gatacgttta aatgcgtttc tgagctgttt
     2881 tggatcagat ggtgatgttt ttagccctac cctaaaaaca aagcccagaa aagaatttaa
     2941 aagcgttcgt acctaatacg tttatattca tatacatata cataaacata aacgtgtata
     3001 cgtttatgtt tatgtattta agttttaggc tacttcctta acatctcctg ctgatagtcg
     3061 agtgaaccga gtgagtgatc catcttgtag tcgaaaaata aagctacgtt tgagtttata
     3121 ctgatagaca gcgacatacg atccatccca gattgagtaa gttttacctg caactaataa
     3181 gtcatcaagt tttggtttat tgctttcttc tttaattcta tctagttgtc gcttaaccat
     3241 tttttcttga tcaccgacaa agaatttcaa ccattttttc ggaattttct taaagtaagc
     3301 aggactgtca tttaagaagt agattggttt gtacatttca tctcgaaaga tgaagctgtc
     3361 aatatcgtag gttccttctg catctttgaa aaggataatt ccatagatct tcgttacttt
     3421 ggtatctacg atttccaaac catttttggc gtaccctttc ttttcttcga tcacttcatt
     3481 tgctgatgaa taatcaccat accatcccat aaaaaatcgc tccttttgaa agttaattag
     3541 cttgtttaca cttatattat acacaccggt gtgtataata tcaagaggat attgttcttt
     3601 tttaggtaaa gtgggatcgt aaacagtgat tttgttgggt taacaaaaaa acagacaagc
     3661 cttatcagct tgtctgttac ttataaactt cacttctgtg accaatagac aatataagaa
     3721 ttgtcacttc atgctcgcta atatcagcta ttatacgata tgcacctacc ctatatcgcc
     3781 agtagcctga tttattagct gttagacctt taccatgctt tcttggatca tcagtgccta
     3841 caagattttt ctcgatccag tttaggataa cctttgcctg aaacttatcc atttttttta
     3901 gcgctttttg agcttcacgt tcatatctta cttggtaagt catcttataa gcctaactct
     3961 tttagcatat cttcgtggga aatactttga tcgttttcct catgctcttt cattagttgc
     4021 ttgtaggtgg caaagtctac taacgtttct gctgtttgca gggaattact acgcataaat
     4081 tcagaaatgt tatctccgtt gaattcagca actttttcaa gaaaatcttt ttcttcttta
     4141 gatacacgaa tacttattac tggcatagtt tatcaactcc ttttcaatta cattgtacta
     4201 cgattgtatg acaaatgcaa tagttatata tttgaatata tacataaaca taaacatata
     4261 catatatatg tttatgttgt agaatataaa agcaaatata aatataaaca cgtttatgta
     4321 tataaacata tacataaacg tgtttatagt gtataatgga gttacaaaag gttaataagg
     4381 gaggtttaat catggaaaag aagttgtcaa tcattactgg aaacattgaa aaaggtggaa
     4441 caggtaaaac aacaacaatc tacaatctag gtggatatgc tgtcgaaaat ttaggtatga
     4501 aggtgctgta tattgatgaa gataagtcgc aaaacctatc aaaacgattt cctgatgctt
     4561 tcagtaaagc gaaaagagag aatacaatca atcatttata tcttcatgga tcagctgaac
     4621 ctttagctat aaatgagaac atggatattt tagtagcagg tgaagattta aaacaagttg
     4681 agatgcaaat gcgtgatatg cctaacaaca gaaccctact gtttagatgg atcgttgtta
     4741 actatgaaca gcttaaagag aagtatgatc taattctgat tgatactcat aatgatgcag
     4801 gtctactagc tcaaaatgct tgggctgtat ctgatcttgt cgtaggcatt tctgatccat
     4861 ctaaagatgg ttttgaagct ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac
     4921 agttgattga tattaagact ggtgaaacac tgatggaaac taaatacgct acgatcggta
     4981 gcaggatcaa acataatact aattcaagtc gtgagttcct agaattggta ccaacgttat
     5041 ctaattatct tggatatgta caagagaagg aattgcttaa tgcaactagc ttagaaggta
     5101 agaatattgc tgagttcgct aaggataaga atatctatcg taagaataag gatttctttg
     5161 aaagcacgtt tacgctatac aacaaaataa ttgatacggc acaagaataa ggagtgattg
     5221 aagtgaaaga aaatagattt aaaaaattcg ctgaagctga agtagaagaa gttcctgaag
     5281 tggttgaagc agttgaagaa cctaaacaaa cacctaaaga aacaacaagt tctttggtag
     5341 ctaaactaaa aagtgatagt tctgaaaaga agaaaaatta tcaattcacg cttcaaccat
     5401 cacaaagaaa gaaattatct aaaatcagcg aaaagttagg aattcgatca gattcagctt
     5461 tacttggtga aatgatagat aacttgtatg atgaagtttt tggtgagtga tcagtttaaa
     5521 gctgatcact ttttttattg gaataagcgg ttaggtatag cttaactaaa tttcgtatac
     5581 gtgtttatgg atatatacgt atacatgttt atatacatgt atacgtatat gtttatgttt
     5641 gtatttgtac ataataaaaa ggctaggtat tgtaacctcg cctttttatt tttaaagtaa
     5701 gtctttgtta cttggtgcat tgaacccttt tagcattggc ttttctttct ttgctgaatg
     5761 atcggatgta accgttgaag gatgctcttg ttgaataggg ttatgatcag ttggtttctt
     5821 ctttacttct gctactttgc tatcgtttct atcgttgcct gcttcaataa ttgtaacttc
     5881 attgttcttt acacgccgat ataggttcaa agcttttttg atcgtctgag agtatttaac
     5941 gcctagtgtt tcatattcgg attcaagaaa ctctgctaaa ggtaactcgc taaccttttc
     6001 tctattgatt ataactacat aacgctctga ttttccctta ttcatgttga tctccttcgc
     6061 tgttttattg agttaaacca aacttgtaaa agccttttac attagaaaat tcactgtctt
     6121 caacaaacaa ggtatgcgga attttatctt ttacaagttg tttgtcgata atagaagctc
     6181 caccaccagt aaagacaact ttatctaagg ttgtgaagtt tttgaatgta gctaaaactt
     6241 ctgcaacaac catattggtg tatgttttta tttctctttc aactacttct gaaatatcaa
     6301 aactttcatt tccgcttggc ttaaatagat atacgccttt ttctttgcct tctcggataa
     6361 tcttttcaag ttggaaagca ttaggactga tcggaaggtc aagcatattt acgatacgat
     6421 tatataaagt aaaaatgccg gtatcctttt gttcacgatt gttttcgtca atttcaaaat
     6481 tttggacaac atcaaaaagt ttcgtgccac caccaatatc aacaaaaccg atcttctgac
     6541 tgatcaatgt ttcatcttgt acactaccat catcagcaag taggaggtta tatagtgttc
     6601 caatcggctg tggcagtacc caaacggtat ctacgtttat attataagcc ttaccgtcaa
     6661 cttcaactga atgttgtcgt gtggcttgtt gcatcacgta tttgacgatt gtttcggtat
     6721 agtcggatga aggcagacct aaaactagat taacggttaa aacgtcatca gattgatctt
     6781 ctaaacgtgc taggttagct aaagagaaag ataggatatt tttgtaggtt tcctgcttgt
     6841 atctgctgat cccaaaacct agtgattcta tcacgtagtc atcttttcct agctcgtgta
     6901 tatccttccc aaaataatag ctttcagtag tgtttactag gctatattca gaaacgttta
     6961 gtgatcgttt taatgaggaa cctatattag aaaaacgtcc tgaattaatg atatttttgt
     7021 tcaaaatacg agagggaagt gagagtggag aaacgtgtgt actcatttct tttactgatt
     7081 tattccctaa gtcaacggaa aacaataatg gttctttcat ggtatgacct ctttctgttt
     7141 gtgtttatgg tatttcggta cgaaaatagt ttacacctat ttactattag ttgcaactgt
     7201 ttataagaaa aaaatgcaat cataaaagtc taattaatta taataaagtg taaataaaaa
     7261 ggtgtaaata aatgcttgca aatttatctt attatgatat tataaaatca taaattatat
     7321 taaggagtga tcctgatgcg attgagaaga aataagaatt acactaccat tattttagaa
     7381 gattttactg atcaagaatt acttattgtc ggtaaaacaa aagcagattt tatcgaaaag
     7441 ggaaatgctt caatagtaaa agcttcaaag gttgctgtcg aaaaatattt tgattcttta
     7501 gaagaaagtg ctaaggaaat ttttaatgga gagaaagctg gtagctatga gttagacggt
     7561 attagattta gcttgatccc aacaactgaa aatattattc cgaagaaatt atattatatc
     7621 ttttcatctg aaaaacatac ttcaatgaag ccttgtattt ttgattggca atggttgatt
     7681 gatgctgtat tggatgtaaa atgttcaaaa tacaatatta ctttggggtg ttaattatga
     7741 gtatgctagt tagaaaatta ccattatctg taagagaatt tctattttac ttggtctgct
     7801 taatcgcatc agggtttaag ctgatcgaac atatttttat ctttgcttat gtattagcaa
     7861 tgatcgcatt tttatctggc ggttggttaa ttatttattt gttaagctaa gttttagatc
     7921 aaaaataatt agtaaattaa acgataaaat aaccgttaat aaatgtaatg aaaggacaac
     7981 gcctaacaat cagcttgtaa taagattata aaataacata atatcaaaaa tagtgctttt
     8041 taaaggacaa attaagtgta tcaacataaa cgtgtatatg tatatgttta tgttgatact
     8101 ttttttgtct taatttttta tttttaaaat tctatttact aatgctgtat agatagtgat
     8161 cagaaaaaac aaaatagcga gattaagcta tttattagtt catatattat attcagatta
     8221 atatgatcca gtattaatct gaaggcaaaa ccaatatatt tctacggact cttgtaaaac
     8281 tatttaaatt ttcctgctaa taaaataacg aaaatagaat atttagcttt tttataccat
     8341 tttacgataa catgatttca tttgtaatat taaaacattt aaatttttaa ttattctttc
     8401 aaattagatc aattaatatc cttataaaag agcataataa atggttttta catcgttatt
     8461 tattgatatt aatctcatcc aaaaaaaatg aaaacgcata cttttatggg tgattgaatg
     8521 tgattttaat acatatacca attaaaagaa aactttttag atttttactt acaaatttat
     8581 aaaaaaacaa gataattgat actttgattt tgcgaacatt ttatcttgcc gcctaattca
     8641 gctttaatat catgattata tcagtaacaa atcgcttttt tagctaattt aagcatcaaa
     8701 taattattct taatcagggg aaaaataact aataaatgga ttttgggaat aggtaaaaac
     8761 ttgatcggtt tctggctaaa aataacgtga aaaaggaaat ataaaataac atggagcgtg
     8821 ggaggaataa agaaatttta cacgaaaggt gcgcagggat gtaaagaaac ttaacacgaa
     8881 acaaaagt
//
LOCUS       DASJST010000112         8715 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJST010000112 DASJST010000000
VERSION     DASJST010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.113"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(4..369)
                     /locus_tag="U4B05_002193"
     CDS             complement(4..369)
                     /locus_tag="U4B05_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN2051310.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(516..1289)
                     /locus_tag="U4B05_002194"
     CDS             complement(516..1289)
                     /locus_tag="U4B05_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2051311.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(1463..2323)
                     /locus_tag="U4B05_002195"
     CDS             complement(1463..2323)
                     /locus_tag="U4B05_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN2051312.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(2323..3741)
                     /locus_tag="U4B05_002196"
     CDS             complement(2323..3741)
                     /locus_tag="U4B05_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2051313.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(3798..4247)
                     /locus_tag="U4B05_002197"
     CDS             complement(3798..4247)
                     /locus_tag="U4B05_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2051314.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(4692..4955)
                     /locus_tag="U4B05_002198"
     CDS             complement(4692..4955)
                     /locus_tag="U4B05_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN2051315.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(5054..6349)
                     /gene="rho"
                     /locus_tag="U4B05_002199"
     CDS             complement(5054..6349)
                     /gene="rho"
                     /locus_tag="U4B05_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN2051316.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(6346..7617)
                     /locus_tag="U4B05_002200"
     CDS             complement(6346..7617)
                     /locus_tag="U4B05_002200"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2051317.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(7783..8652)
                     /locus_tag="U4B05_002201"
     CDS             complement(7783..8652)
                     /locus_tag="U4B05_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN2051318.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         2387 a         1939 c         1523 g         2866 t
ORIGIN      
        1 gatttattcc attttccgtt catctggtac gatgccttga cttagcaacc acttgtggaa
       61 gaaccattct gccacaccga tcaatagagc aggaatcaat actttcatcc aaatatcgac
      121 tgtatcattc ataaaatata gatagagcca caagataact gcagctgaac cagcatctgc
      181 gatcgtcgct gcgatgttcc ctactttacg taagatcaac agatccccta gcacatagat
      241 catgactaca ataattaccc caacaatcgt tgaatccata aagctaacac cgtataaacc
      301 tgtcagcacg acccacagaa tcgcccagat catgattgcc ttcactacca acgccttcac
      361 atgtttcatt ttctgcactc ctttctcctg gataaaaaag taagattatc taacaagata
      421 accttttcat tattatacca tcggctgaga gaagcgagca aagataaggt cctatctaaa
      481 aatgaggaag ccacttagaa cattcacgta ataatttatc cgtacagaca ttcgatatgg
      541 tgttccttta ctttcgcata ctgttctttt gtcagttctt tacttgtcac cagataatcg
      601 atttcatgaa aagcaatggc ttgatgcatg aatttatgtg caattttttg ctgtgtaact
      661 aaaacggcaa gccggtctga ttgttttcta aaagcttttt tgaccattgc ctctccttca
      721 tgagtttctg ttactccttg gctgaaatct agcccgcgac aactcattat cgctaaatcc
      781 gcataatatg acaatgcatc gttataggct tttgaaccgt tgattgttgc tcgtttcgga
      841 acaattgccc ctcccaatac agatactttt gccgttgtat attcactcaa catagcagct
      901 gtgacgatgc cattcgtaat gatctgcaaa ccatcaaatc ttgctaggtt tctgactaag
      961 tgtaaagagg ttgtactgga atccaagaat aagaccatat catctcgaat caaagaagaa
     1021 gctgtatctg caatcttacg tttttctgtt tcttcttcta atttggtgat cttcatcagg
     1081 taaggcacgt caatttttcg attgtcaata agcatggcgc caccgcgaac tctacggatc
     1141 aatccacttt tttccagaaa aacaaggtca cgtttaatcg tggcttcgct ataatttact
     1201 aactcgatca aatcggtgac tcttgcaaat tggttgtttt ttagataatt cacaatctcc
     1261 tgctgtctat tctccacttc attcaacata aaatcacccc atttacttta tatagtaaca
     1321 ggaaaaaaga ggttgtgaca gaagtgattg gcttcgagaa ataagaagaa atttttcgaa
     1381 aaaactactt ctgaacatcg tgtgtggtta agagggtatg acaagactat tgtcataccc
     1441 tcttgaccac acatttttat ttttagtata aactagcctt gcctactgtg ttcaaaattt
     1501 ccattttatg tttgaccact tcttgtacct ttttattagc tgctggataa attgcattcg
     1561 gctcgtacat cgatggattt tcactcaata tttctctcaa agcattgaaa aagacgcttt
     1621 taagatctga gctcaaattc actttggcaa ctccgtattt tactgcttcg ctgacttctg
     1681 cgtccggatt acctgaaccg ccatgtaaaa caaatggaag atcgatttgt tggttcaatt
     1741 ctttcagtaa gtcgatattt aattttggag ttctgttttt cggatacaag ccatgcgctg
     1801 ttccaattgc tacagcaagt gtatcgattc ctgtttcttt cacaaaaatc ttcgcttgat
     1861 caggatctgt ataaatgatt gtttcagcac ctccttcagc agaaccatta ttcccaatcg
     1921 ttccaagctc agcctccact gagacattta ccgcatgagc taacgcaacc acttttttcg
     1981 taatctgtac attgtcgtcg aatgatttca aagacgcatc gatcatcacg gaagaatagc
     2041 cgtttcgaat cgcccgtaag acatcttcta tcgtaccgcc atgatccaag tggataacga
     2101 tcggcagttt acttgtataa gcataggctc ttactgccgc aacaaactca tcacttaaat
     2161 aggcgatttc atcaggatga atttctaaga ttactggtgc ttctaatctc tctgcttctt
     2221 cgatgattgc tttcatcata tcatagctgc aaatattgaa ggctggtact gcaaattgat
     2281 tctctttagc cactttcaat aactctttca ttgtatataa cattacgctt ccacctcatt
     2341 tgttagattt tcatttttcg tccacttgtc ttgttttttc tctttcactg tttttttcaa
     2401 tagtccaagc ataaaggctg tagtgactgt acctgatgca attgccaaga catacatagg
     2461 taaattgttc ataacatttg gaattagcat cacccaaatc cctccatgag gtgccattga
     2521 agtaactcct actgccatgg aaatccctgc agcgactgct gaacctgtca tcaaactagg
     2581 tatcacgcgg atcgggtcag ataccgcaaa cggtatagca ccttctgtaa tatacgaagc
     2641 gcctaaaatc cagcatgatt gacctgaagt ccgttcttct ttggtaaatt tatgtttgaa
     2701 taataaagtg gctaaagcaa tacctagggg aggtgtcatt cctgcagcca tacaagcagc
     2761 aatcggtgca tttactccag ctgacatcaa accgatcgag aaagtcgaag tcgatttatt
     2821 gatcggccca cccatatcag ctgccatcat gatcccgata agtaatccga acaatagccc
     2881 ggatgtattt cccaagctat tcagccaatt tgtcaaagca tccaacaccc atgtgatcgg
     2941 ttgttcaatc acaaatacca tcaaaagccc aataattaag atcgataaaa aggggacgac
     3001 aatcagctga taaatagcag aaaattcttt ttttacgcta attttatttg ccagaaaatc
     3061 acagatatac cctgctagca cgccaccaat cattccgcca agaaaacctg aaccacctgt
     3121 agaggccagt aatccggcaa caattcctgg ggcaaaacct gcttttccag caatcgatgt
     3181 agcaatcccc gcgcctaacg caggaaccat catggcaaac gctgtatctc ctccgatcct
     3241 tgaaaatgca gctgctaaca cattatagtc tgctgagttg ggatcagccg ctgtaatccc
     3301 aaaggcaaaa ctcagtgcga tgatgattcc gcctgcgatc acaaatggaa gcatgtaatt
     3361 cacaccattc atgaaatgat tatagattgt cggctttttc tcatttgcag ctggttcttt
     3421 ttcattagac acaacgaata cgccaatatc tctcaatgct tcgtctatta ctacttgcgg
     3481 ttctttgatt gctcggcttg tagagacacg ctttactttt ttcccggcaa atcggtccaa
     3541 ttcgatttct ttatctactg caagaatgat tgcatctgcc tctttgattt cttgttcaga
     3601 taactgattt tctgttttta caccttgtgt ttcgaatttt gcttgatggt ttagatttct
     3661 tgctgttact tctaattttt ctgccgacat ataagtatga gcaattccag tcgggcaagc
     3721 agttacacca acgattttca tgattatttt cctccttttt cttcactatc gatactcgga
     3781 caaagactct actattttca ttcttgacta atctgtctga tgatatctgc tggcgtagtg
     3841 gcttctttta ttccctttct aaaatctgca ttcatcaatc gtctggagag tgtgctgaga
     3901 atctctagat gcatatctgc tgatttttca ggaactacga tggcaaaaat atattcaatt
     3961 ggcatttcat ctatcccgct atagtccgtt aatttggctg tatgaaaaaa acacagtgct
     4021 ggttttgtca catatttgct ttttgcatgg ggaatcccaa atccatcttc tattcctgtg
     4081 gagatctcct cctctcgttt gaatagtgcc ttaacgactt tttgaacatt tcttacctta
     4141 tttgtttttt tcaacacatc ccctattcgc tcaaataatg atccttttga atctatcgat
     4201 ggatcgaaaa caatcagttc ttctgcgatc aattgatcaa acctcataaa aaacctctcc
     4261 cccacaaaaa atgttaacgt tttcttggaa ctgaggtcag tatagcatcg ctagctgtac
     4321 cgttcaatat tatgaatgaa cagaggaaaa agacccatca tccgcgtgta taagtcatta
     4381 aaatgaacga tctagctcac aaacaatcgc aagtctataa caaaacaaaa agccttcctt
     4441 gaaaggaaag cttagcttct aattttaggt aaatggtttt ttacttcgaa ataacccctc
     4501 ttcatttcgt taatataagg tgtttttatc aagtggataa ttaaaactat atactagttt
     4561 ttcattgttt tgtgcattat gatcctacga ctttttcagc atcaaacgaa gttttaaatt
     4621 ggcaaaaaac ataaaaaaac agactatccc tatcagatag tctgctaaac gttgagttat
     4681 cagcgttttt attattccgc agcgtttgcg tctttgagac cgtatttttt gttgaaacgg
     4741 tccacacgtc cgtctgcttg tgtgaatttt tgacgtccag tgtagaatgg atgtgagtca
     4801 gaagttactt cgacacggat gactgggtat gtattaccgt cttcccattc aactgtttct
     4861 tgtgaagttt ttgttgaacc tgacaagaat ttgaaaccag ttgttgaatc catgaatact
     4921 actgggtggt aatctggatg gatgttttct ttcatgattt tttcgctcct ttgccctgct
     4981 ccatttgctg aaccagagtt gatttgtcga tttacaacat taacatctta acatgtttca
     5041 gtcaaggttg caattatctt ttcagatttc tggtcggatt ttttttacca aaggagacat
     5101 cttgaaattc tttgaaaaat tgctgattgt tttttgtttt acgcaaaaag cggatcagtt
     5161 gttccgtata ttctaatgaa tctccagtca tattttttcg aagtttccaa atttcttcta
     5221 attcttcact tggcatcaat aattcttctt ttcgggtacc tgattttttg atatcgatgg
     5281 ctgggaagat ccgacgttca gataattcac gtgaaagttg taattctaga tttccagttc
     5341 ctttgaattc ttcatagatc acgtcatcca tacggcttcc tgtatcgact aaagcggtcg
     5401 ctaaaatcgt caaactgcca ccttcttcga tgtttcttgc tgccccgaaa aatttcttag
     5461 gtttatacaa tgcggctggg tcaatcccgc cactcaatgt acgtccgctt ggtggaacga
     5521 ctaagttata ggcccgagca agtctagtaa tactatccat caagataacg acatctcgtt
     5581 tatcttcgac taaacgcatt gcgcgttcca atactaattc agaaactcga gtatggtttt
     5641 gcggctgcag atcaaatgta gaagatacca cgtcgccttt cacgcttcgt tctagatccg
     5701 ttacttcttc cggtcgttca tcaatcagta gaacgattaa ttctacttcc ggatagtttt
     5761 cggtgatacc atttgcgatt tcttttaata cagaagtttt ccctgctttt ggcggtgcga
     5821 cgatcaatcc acgctgacca aaaccgattg gagaaaagat atcgatcatt cgggtagata
     5881 gtctttgcgg tgttgtttcc agttgaagct gtctttgcgg ataaagaggt gttaaggctg
     5941 gaaaatgcgg acgttctttt gcttcttccg gatcttttcc attaacgctt tccacgtgca
     6001 tcaaaccata ataacgttcc gattctttag gtggacgggc ttttcctgcc actttgtccc
     6061 catttctcaa actgaatcgt ctgatttgtg aggcagaaat atagatatct tctgcacttg
     6121 gtccataatt gattggccgt aaaaaaccgt aaccatcttg accaacgata tccaatattc
     6181 cttccatata ataaaatcct tgtttctctg cttgtgcacg aatcacagct agagataatt
     6241 cctttttatt catcttactg taataaggaa ttttaaattc tttggcataa ctataaactt
     6301 cttttaacgt tttgttctct agttctgcca ttgtcaaata atcgctcatt cagctcccac
     6361 ttcctctatc atttcaattg atgcccctaa ggctgttagt ttctcgataa tatgatcata
     6421 accgcgtaag atataatcta cattgtaaat cgtcgttgtc ccttctgcca atagtccagc
     6481 aatcactaaa caagcacctg cccgaagatc agaagccacg acttctgtgc cgtgaagttt
     6541 atttggacca ttcacgatga tcatatttcc ttccacggta atatcagcgc ccattcgtgc
     6601 tagttccggc acatggttca cacgtttcgc atagatcgta tcgatgattt ctgaagtgcc
     6661 tgtagtcatc aatagtaacg cagtaagtgg ctgctgcaga tctgtggcaa atcctggata
     6721 aggataggtc atgatgtttg cgggttttaa ttctttcgag gggtaaactt cgatttcatc
     6781 ttcgctgatt ttcatatgga caccaatttc ctgcaatttt gctataaagc tttccaaatg
     6841 ttcaaagatc acatttttca ctttgatacc atttcctact gcagctgcta atgctagata
     6901 ggttcctgct tcgatgcggt caggaatcat gaaatgccga cagccatgta atgtttcgac
     6961 tccttcgata cggatcacat ctgtacccgc tcctcgaact tttgccccca tattattcag
     7021 taaagttgcc acatctatga tttccggttc tcgtgcagcg ttttcaatta ctgtctgtcc
     7081 ttttgctttt acagcagcta acatcacatt gatcgttgct ccgactgaaa ccacgtccat
     7141 gaaaatccga ttgccacgca atcctttatt ttctgtccgt aaatacatgg caccatgttc
     7201 atttgtcact tcagcaccta gagcttcaaa tcctttgacg tgtaggtcaa tcggtctagg
     7261 gcctaaataa cagccacctg gcaggcctac taccgcttcg ccaaatttac caagcagtgt
     7321 tcccataaaa taataagaag cacgcaaact attgattttt ccgtgcggca tcggaatcga
     7381 tacgacttct cgaggatcga tttctagtat gttgttggaa aaatggacag ttgcgcccat
     7441 gatctctaaa atttcaatta atgaatgaac atcttgtatg tccggcacac cttccaagat
     7501 cactggggaa tcagctaaga ttgcagctgg aatcaatgcg actgcactat tttttgcacc
     7561 gctgatcgtt acttcaccag ctaaaggtcg gtttcctttg attacgattt ttttcatctg
     7621 tttccacctt gtttattaat cttttttcat ggatatgctt gtattttatc atagataaaa
     7681 aaaaagaaac aagcatggtc tgccgtttta agtaaaaaag aatggagagc gacttcaaaa
     7741 gtcgttctcc attcctatat atcgtatctg ttatgtggat gcttacgctt tgttagctga
     7801 accgaaccag tcgatatgag atttagcatc ttcaacgatt gctgctttac ctggagctaa
     7861 taatttacga gggtcgaatc ctttaccttc tttatcttta ccagcttcga tatatttacg
     7921 agtagctgca gcaaatgata attggaattc agtgttaaca tttacttttg agatacccat
     7981 tgagatagct ttttcgattt gatcttgagg aatacctgat ccgccatgta atactaaagg
     8041 catgtctgga cctacagcat cagcaattgc ttgtaagtgg tcaaaagcta atcctttcca
     8101 gttttctgga tattgaccat ggatgttacc gataccgcaa gctaagtagt cgatacctgt
     8161 agcaaccatt tgtttgcatt cttcaatatc agctaattcg ccgttaccga tgattccgtc
     8221 ttcttctccg ccgattgaac caacttcaca ttcaactgag ataccttttg catgtgcttt
     8281 ttcaacaacg tctttagcta atttcaagtt ttcttcaaat ggtagatgtg aaccatcgaa
     8341 cattactgaa gtatagccta attcgataca ttccaaagca gcttcgtaat caccatggtc
     8401 taagtggata gcaactggaa cagtgatgtt cattgcttca actaaatcag tgattaaatc
     8461 tttacagatt ttgtaaccac ccatgtattt agctgcaccc attgatgttt ggataagtac
     8521 tggtgcttgt tttgcttcag ccgcttcaag gattgcttga gtccattcca agttgtttgt
     8581 gttgaatccg ccgacagcgt aaccgccttt acgagcagct tttaaaaatt cagctcctga
     8641 tactactggc ataataaaat tcctcctaag ttttgtaaaa ctaaatttgt taagacaatt
     8701 cttaaccaac gaccc
//
LOCUS       DASJST010000113         8546 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.114, whole genome shotgun sequence.
ACCESSION   DASJST010000113 DASJST010000000
VERSION     DASJST010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.114"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="U4B05_002202"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="U4B05_002202"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN2051319.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="U4B05_002203"
     CDS             complement(1098..2201)
                     /locus_tag="U4B05_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN2051320.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="U4B05_002204"
     CDS             complement(2291..3247)
                     /locus_tag="U4B05_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN2051321.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="U4B05_002205"
     CDS             3528..5462
                     /locus_tag="U4B05_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2051322.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="U4B05_002206"
     CDS             5919..6335
                     /locus_tag="U4B05_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2051323.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="U4B05_002207"
     CDS             6349..6915
                     /locus_tag="U4B05_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN2051324.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="U4B05_002208"
     CDS             6938..7924
                     /locus_tag="U4B05_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2051325.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8546)
                     /gene="scm"
                     /locus_tag="U4B05_002209"
     CDS             complement(8079..>8546)
                     /gene="scm"
                     /locus_tag="U4B05_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN2051326.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2519 a         1644 c         1735 g         2648 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt tacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgtggt agaggtagta
     8521 tctgtactgt cacttgaagc tgatgt
//
LOCUS       DASJST010000114         8493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJST010000114 DASJST010000000
VERSION     DASJST010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.115"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="U4B05_002210"
     CDS             complement(<1..173)
                     /locus_tag="U4B05_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2051327.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U4B05_002211"
     CDS             complement(593..1714)
                     /locus_tag="U4B05_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN2051328.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U4B05_002212"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U4B05_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN2051329.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U4B05_002213"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U4B05_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEN2051330.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U4B05_002214"
     CDS             complement(4304..5215)
                     /locus_tag="U4B05_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2051331.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U4B05_002215"
     CDS             complement(5232..6239)
                     /locus_tag="U4B05_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN2051332.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U4B05_002216"
     CDS             complement(6236..8365)
                     /locus_tag="U4B05_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2051333.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="U4B05_002217"
     CDS             complement(8370..>8493)
                     /locus_tag="U4B05_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2051334.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DASJST010000115         8421 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJST010000115 DASJST010000000
VERSION     DASJST010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.116"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            471..2108
                     /locus_tag="U4B05_002218"
     CDS             471..2108
                     /locus_tag="U4B05_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051335.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="U4B05_002219"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="U4B05_002219"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2051336.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="U4B05_002220"
     CDS             complement(3184..3819)
                     /locus_tag="U4B05_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN2051337.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="U4B05_002221"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="U4B05_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN2051338.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="U4B05_002222"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="U4B05_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6417..>8421
                     /locus_tag="U4B05_002223"
     CDS             6417..>8421
                     /locus_tag="U4B05_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2051339.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQ"
BASE COUNT         2782 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa a
//
LOCUS       DASJST010000116         8415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJST010000116 DASJST010000000
VERSION     DASJST010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.117"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            193..1716
                     /locus_tag="U4B05_002224"
     CDS             193..1716
                     /locus_tag="U4B05_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN2051340.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            1953..3293
                     /locus_tag="U4B05_002225"
     CDS             1953..3293
                     /locus_tag="U4B05_002225"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN2051341.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3446..4183
                     /locus_tag="U4B05_002226"
     CDS             3446..4183
                     /locus_tag="U4B05_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN2051342.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4348..4569)
                     /locus_tag="U4B05_002227"
     CDS             complement(4348..4569)
                     /locus_tag="U4B05_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN2051343.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4600..5631
                     /locus_tag="U4B05_002228"
     CDS             4600..5631
                     /locus_tag="U4B05_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051344.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5618..7021
                     /locus_tag="U4B05_002229"
     CDS             5618..7021
                     /locus_tag="U4B05_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN2051345.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            7087..7722
                     /locus_tag="U4B05_002230"
     CDS             7087..7722
                     /locus_tag="U4B05_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2051346.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7771..>8415
                     /locus_tag="U4B05_002231"
     CDS             7771..>8415
                     /locus_tag="U4B05_002231"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN2051347.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIK"
BASE COUNT         2780 a         1438 c         1807 g         2390 t
ORIGIN      
        1 aaaggaaacg ctacaatgaa aaagaagaaa gctttaaaaa cctgaaaaaa gaggagggaa
       61 tagggaaaaa cgaacttttt gaacaaaagt tttacaaaat gcgtgttatt atttttgttc
      121 tgatactctt tttgataaaa taacattaga gccaaaattg ttgtctctaa cggaaaagaa
      181 aggaagaaga agatggaaca aacaaaaaag aaaaaatttc aaatgccttc agcgtatacg
      241 gtcttattta ttattattgc agtaatcgct gtacttactt ggtttatacc agctgggcac
      301 tatagtgtag acgatgcagg caacatcgtc gctggatcat atgaacgagt acaacaaaat
      361 cctcaagggc tgtgggatat tttcatggcg ccaatcaatg ggatgctagg tgtaccagcg
      421 ggggagctaa ctaaagaaac acctgctgca atcccaatct ctttcttcat tttggttgtt
      481 ggtggatttt taggtattat caacaaaaca ggggcactag atgccgggat tgcttcagtt
      541 gtgaagaaat tcaaaggaaa agaaaaaatg cttattccag tattgatgct gttatttgca
      601 ttaggcggct ctacttacgg aatggcagaa gaaacgattc cattctatgc tttactgatc
      661 ccagtcatga tggctgtagg ctttgataca gtagtagctg tagcaatcgt attagtaggg
      721 tctcaagtag gctgtctagc ttcaacggta aatccttttg caacaggcgt agcttcacaa
      781 gctgttggga tttcgatggg tgaaggtatt ggtcttcgat tcttgatgtt cgttattttg
      841 ttagctattt ctattgtata tgtgtatcgt tatgcatcaa agatcgaaaa agatccaaca
      901 aaatcattag tatacaacca acgtaaagaa gatatgaagc atttcgatat tgaagcaatc
      961 ggtcgtcaag aagtaatcac gaaaaaacaa aaacatgtta atgtattgtt ctttattact
     1021 tttggtatca tgatcatcag tttgattcct tggacaacct tgaatgcgaa tttcacgatt
     1081 tttgaatcat tgacaaactg gttaacaaac cttccggtac taggtacggt tctaggtaaa
     1141 agcatgcttc cattaggtga ctggtacttc ccagaaatta ctatgttatt cttagtaatg
     1201 tccatcgtag tagcaatcac ttatggcatg aaagagtctg aatttgtctc tgaattttta
     1261 gctggtgctt ctgatttgat cggtgtagca atcgtagtag ctgttgctcg tggtatccaa
     1321 gtggttatga acaatggatt aatcacagac accatcttac attggggcga acaaggatta
     1381 agttcattat cttcaagtgt attcatcatt attacattta ttttctatat ccctatgtca
     1441 ttcttgattc cttcaacttc cggcttagct gctgcaacaa tgggaatcat gggacctatg
     1501 ggtgaatttg ccggcgtcgg taaggattta gtaatcactg cttatcaatc tgcatctggt
     1561 ttagtaaact taattacacc aacatctgct atcgtaatgg gtgctgtagc aattgctcgt
     1621 ttcgatgttt cagtgtggtg gaaatttacc ggtaaattaa ttgcgatatt atttgtagcc
     1681 atctgtgtga tcctaggtgt agcagcaatg ttctaaacct agacaaatac aggtacaagg
     1741 tctatcataa gaatgaagaa accgataaac ggctatcgat aagcacgtgg ataagtcaat
     1801 agctccattc agtgagtgtt ttttctttca tcatttgtta agaacattct gcttgctgat
     1861 ggagcaggct taaaacgtca ttatttgaga agaagaggtt gtgtcggagg aatttgacat
     1921 gacccttttt aacatattag gaggataata tcatgaaaaa atttgtgaca gaacaacatc
     1981 atgaacaagc attagaatca ttgagcgaat tgatccgaat tccttccgta ctagatgaag
     2041 cagattcagg acaaggacat cccttcggta aaaaagtgat tgaagctttg gataaagtat
     2101 tggaaatcag tgaaaagcta ggatttagaa catttaaaga tccagaaggc tattacggtt
     2161 attcagagat tggctctgga gatgaattat tcggtatctt gtgtcatatg gacgttgttc
     2221 cagcagggga cgaaaataat tgggaaacaa aaccattcga tccaactata aaagacggct
     2281 ggttagttgg tcgcggatcc caagatgaca aaggtccttc tattgcggca atgtatgccg
     2341 tcaaagcttt gattgatgct ggcgtggaat tcaagacacg tattcgtttt atctttggta
     2401 cagatgaaga aaatttatgg cgttgtttag aaaaatataa cgaaaaagaa gaaggaatta
     2461 cgcaaggatt tgcgccagat gctgaatttc ctttaattta tgcagaaaaa ggcttattgc
     2521 aagcttactt gacaggacca ggaacgaacg agttttctgt aaaagctgga ggagcgttga
     2581 atgttgttcc tgatgcagct ccttactctg gagaaaagct ttctgaagta aaagaagcat
     2641 tgaaaaaaca cgaattcgat ttcgaagaac aaggtgaagg catcgtcgtt cttggtaaaa
     2701 gtatccacgc gaaagatgca gcacaaggag tgaatgctat ttctcgtctg gcaattgctc
     2761 tttcagaagt atttgatttt ggcccaatca atttcttagg aaaattagtt caagaaaatg
     2821 caactggtga agctgtcgtg ggcaaaacag aagatgaaca atccggcgag ctaacgatga
     2881 actttgcaag tttggaaatc acaccagaac aaacaaaaat cggtgtagat atgagaatcc
     2941 ctgttacatt caagaaagat gatttagtag ctaaattaac taaaacagca gaaaaatatg
     3001 ggttgactta tgaagaattt gacttccttg attcattata cgtgccacta gacagtgagt
     3061 tagtgaaaaa tctattgggg acatatcgcg atattactgg agacatgact gaaccgtttg
     3121 tatcaggcgg cgctactttt gctcgtacga tgaaccagtg tgtggcattc ggtgcgatgt
     3181 tccctgatac tcctgacttt atgcaccagg cgaatgaacg atgggaatta agtagtatgt
     3241 ataaagcaat ggaaatttat gcagaagctg tgtatcgttt atgtgctaaa taaaaataaa
     3301 catacaaaaa gggcggatat tcaaatggat attccgtctt tttttatttg atgctggaaa
     3361 gtttgcattt ctctcttatt tttttaaggt ataatcatac ttgattaaga aaaaggggtc
     3421 actcagatag aaaaggtggg agaaagtgaa aatatttaga cgaatactaa ttggcttagt
     3481 catcatttta ggattactcg gtggagccgg ttattactat atacagcaaa atacgtacga
     3541 accatcgtca gaagcgcaag tgttggtaaa agaaggaaaa aacaaagaag attacttatt
     3601 ttttgaagga gagaaaaaga atcatggaaa gtctccatta cttatttttt atcctggtgc
     3661 tctcgtagag ccagaaagtt atagtatttg ggcgcaagat ttagcaaaaa acggcttttc
     3721 agtcgctatt gtcaaaatgc cacttgatct agcagtgatg ggcggaaaca aagcggataa
     3781 ggtcaaagaa gattttccaa attccgatta tgtgatcggt ggccattcat taggtggcgt
     3841 aatggcaagt cgttatgtca ataagaatca ttcagacaaa catttaaaag gggtattctt
     3901 catggcaagt tatcccgaca aaaaaggaac attgacagaa tcagctgttc ctgttttatc
     3961 gatcacggcg acaaaagata aagtattgaa tcaggaagct tatcaagaag ctaagcagta
     4021 tttaccggaa agaacagagt atgtatcaat caatggcgga aatcatggag gttttggcag
     4081 ctatggtgag caaaaaggag atggagaggc agaaatttct aacgcagacc agcaaactgt
     4141 gatcgtcaat acattggaaa actggctgaa aaagttgaaa taatataaat gggtcctata
     4201 gggtccgtga aaaaactagt caattaacta tagatagcaa atgaatcttt caaaataagt
     4261 cgggatttca cggaacaaga gaaacagatc ccggcttatt ttcaagttta gagaacttag
     4321 tgtacgtttt taaagtttcg tttatattta tattttattt tctaataaaa tcgctcgaac
     4381 tggacatttt tgatacgctt tttttacata tgtttcttct tccggcatca cgaactgttg
     4441 atgagcctct ggttcttggg caaataaaac gattccttct tcatcataat cgaaaatatc
     4501 tggcgcttcg atttgacaaa gaccgcatgc aatacagcgt tcaggtacaa gtttacatag
     4561 tagtgacatt tctaaccatc ctaacttaag gagaataata tgattgtttt tattttatcg
     4621 ctattttcat caggacacaa gctgcggatt agttctcttt accaactcct tgtgggaaaa
     4681 cgaacgactt cagtacttat ttatggattt acccacgaac ttttgtttat ccataacagt
     4741 tttccagaat tgaagcagga taagttttat caaattttgc aaaaaattgc gcagcaaggt
     4801 tggatcgaaa taaatgaaaa tgaagcaaag ctaacgcctg caggggctga tatgttgtcc
     4861 gaacatcaaa tagaacatac tggtttgcgt tttgaccgct atggcagaac aggggaaact
     4921 agttggcggc tcattaaatt tgctgttcaa gtaatttcta atctggcaac gggtattcaa
     4981 gactatcttc cagcagagac aagtcctttt tatactttcc aactaaaaaa atggcttagt
     5041 gaaagccaat tgccacgtga aattttgatt gatactgcgt atgagagttt ggtgcagctg
     5101 ttttcggaga taccagaaga agcagcagat tttttagcta accaattatc tggaaatgag
     5161 cgaacagggc tgcttcctta tcagctggca aaaaacaatg atgagtcagc ggtttatctg
     5221 caacaaagca gatgtatcca ccttttgtta gcacaaatcg aaaaacggcc agattcgctt
     5281 tggcacgctt tgatcgattc attattacag caaaatttca atcaaagcat gatgatcacc
     5341 aagcagatgt ttatgaatgg tcagacaatt gaccagatca tggcaatccg tcatttaaaa
     5401 agaggaacag taacagatca tcttattgag tgggccttgt tttttgatga ttttccatac
     5461 gagtggattc tctctgcaga aacaatcgaa cgtttggaac caaataagga ttccgtgcga
     5521 gagtggcgct ttagcgaatg gaatgtagat ggacaattag attatggaga atttcgtttg
     5581 tatcaaatct atctattgag aaaggaagcg atacagaatg ttaataaatg aattaaaaaa
     5641 atattttgga tttgattcat ttcgtcccgg ccaagaagaa attattcagg cgttgcttga
     5701 tggagaagat actctcgcta ttttgcctac aggaacggga aaaagccttt gctatcaatt
     5761 gacggggtat ttaatggaag gtctggtcat catcgtgtca ccgctgcttt ctctaatgga
     5821 agatcaagtg acgcaattac aaaaacgagg ggagaaacga gtagctgctt tcaatagttt
     5881 actttcgcaa tctgaaagaa gatacgtcct ccgtcatcta tcgcaatata aatttttgtt
     5941 tttgagtccc gaaatgttga ctaatccttc tcttttagag catctgaaaa aacaaaatat
     6001 tgcgttatat gtagtagatg aggctcattg cgtgtctcaa tggggagttg atttccgtcc
     6061 agaatatcaa cagttaggaa aaatccgaaa gcttttagga aacccggtca cactggcact
     6121 aacggcgaca gcaacagatt tagtagcaag agatatacgt catgtactat ttgatcgacc
     6181 accaaaagag atcagacaat cagtcaatcg gcaaaatatc tcgctttttg ttcggaaaac
     6241 gcagcaaaaa gaacaagaac ttgaacactt catggaaaga gcacaaggag cggcaatcat
     6301 ttattgtgca acaaaaaaag aagtcgaacg attgtatcat ttattcaggg aacgttttac
     6361 agttggatac tatcacggtg gtcttgatgc agctcaaaga agacaacttc aacagcagtt
     6421 tgtaaaaaat cagctgcagt ttctcattgc tacgaatgca tttggtatgg ggatcgataa
     6481 atctgatatc cgttatgtgg tccattatga tttgccagac agtctagaaa actatgtaca
     6541 agaaatcggt cgagcaggca gagatcagga agaaagtgca gcaattcttt tttatcaaag
     6601 tggggatgaa cgtatccatt atttctttaa tcaactttct agagaacagc gacaaagttt
     6661 tgagctttat ttggaatatg ccacagagca agctcccttt gatgagctgc aaaaaaaatg
     6721 gatggagctg atccagcagt ctgaaaaacc agaaaattgg gtggaacggc tcaaacgtca
     6781 ggaaaaagaa aaagaattcc gcctgcagca gatgcttcgc tatattaatg aagaaaactg
     6841 ccgcagaaaa ttcatcttag cttattttgg cgaaaaactc tccgaaaagc cgcagaattg
     6901 ttgtgatatt gatggagcaa aagcagaact tctgccaaag aggaagcttt tttctaagac
     6961 tgaacaactt cattggggaa cgattttact aaatttattt aaaaaaaaca tggaacattg
     7021 attttttagg acaagacact ttttcctatg gtataataca cacgatagag tttaggaggt
     7081 aaaacagtga gcagaaaaga tagacatcaa tctacagaaa ataacgaaac acaagaacca
     7141 tgggaacaac caatctacga tacagatgat gaagtttcat caagaagttc tcagagacaa
     7201 caaaagaaag gaaatacatt atttctttcg ctatttttga ttttactggt cttatgtatc
     7261 gctattccag caggtgctta tttctggatc agaaatggat caagcaacaa tgcaacagca
     7321 gcaacttctt ctgaaacttc gacatcagta gtagaaagtt ctacaagtac aacaaaagag
     7381 gagtcaactg agacttcaac agtagaatct agtacaatcg cggaagaatc tactgtgtct
     7441 caagatacag gtgtaacgtc agaaagcagt attgcagcag agacaacacc gacttcctca
     7501 agtgaggcaa cttatcaagc aggggattca agcagcaatg cagcagaaac aacaccttct
     7561 tcatcagctg cagccggttc aacaacgact gttcaagcag gcgaaggtcc aaaacaagtg
     7621 gctgagcgtg cagggatcac cactgatcaa ttgtttgaat tgaatggctt agatccaaac
     7681 aactttatgc tttaccctgg tcaagaatta agaattaaat aataatagcc gcgctttgcg
     7741 gctttttttc tgagaaaaag gagttatatc atgacacaaa tcagtattgc aatcgacggg
     7801 cctgcttcat ctgggaaaag taccgttgcc aagatattag caaaagaatt gaattatatt
     7861 tatacagata ccggtgccat gtatcgcgca gtaacttatt tagctattac gaacaatgta
     7921 tctttttcag atgaagaagg gctagtttcg ctgatcaaaa agtatcctat ctctttttcg
     7981 caacaagaag atggacagca tgtattcata gcatcaaaag atgtgacttt agaaatccgt
     8041 caaccagatg tcacaaaagc tgtttctgaa gtctctgcac atggaaaagt cagagaagaa
     8101 ttagttgctg tccagcgaaa aataggggaa cgcggtggcg ttgtcatgga cggaagagac
     8161 atcgggacag ctgtattacc gaatgcagaa gtgaaaatct ttttagtagc aagcgtaagt
     8221 gagcgtgcag aaagacgttt caaagagaac caagaaaaag gaattccgac tgatcttgaa
     8281 acactgacga aagagataca ggaaagagac cattatgatt caacaaggga cgtctctccg
     8341 ctgaagcaag cagaggatgc tgttcgtatc gatacgactg gcaaatcgat tccagaagtc
     8401 gttgcagcaa taaaa
//
LOCUS       DASJST010000117         8251 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJST010000117 DASJST010000000
VERSION     DASJST010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.118"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            628..2721
                     /locus_tag="U4B05_002232"
     CDS             628..2721
                     /locus_tag="U4B05_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2051348.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            2881..3747
                     /locus_tag="U4B05_002233"
     CDS             2881..3747
                     /locus_tag="U4B05_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2051349.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(3769..4206)
                     /gene="ndk"
                     /locus_tag="U4B05_002234"
     CDS             complement(3769..4206)
                     /gene="ndk"
                     /locus_tag="U4B05_002234"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN2051350.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            4865..7543
                     /gene="mgtA"
                     /locus_tag="U4B05_002235"
     CDS             4865..7543
                     /gene="mgtA"
                     /locus_tag="U4B05_002235"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN2051351.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            7630..>8251
                     /locus_tag="U4B05_002236"
     CDS             7630..>8251
                     /locus_tag="U4B05_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2051352.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEG"
BASE COUNT         2686 a         1417 c         1743 g         2405 t
ORIGIN      
        1 tatcaagagg tcttgagcct gccgtttaac tctgagccac aaggagcaat ttcaaaaaat
       61 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      121 gtttaccaag aggttgtaaa attagcgtcc tgacataatc taataccgag ggtcagctga
      181 ttgaacaccg tgtatcaaga ggtcttgagc ctgccgttta actctgagcc acaaggaaca
      241 atttcaaaaa acagcttctc atgttttata aatattgtga cttgtggccg aggagttggc
      301 aggagaacac cgtttaagga agaagttgtg aaaaagctgt cctgcatcaa gaaataagga
      361 gctgcgacaa gaattttgtc acagcctctt tttttgtacg aataccgaga aactggcttt
      421 tgaataccgt ggagtgaagc ggtcaccaca agacttttat ctcttcatta gtttaatcgc
      481 ttacatcgca gcggttttat gctgtgaaaa gtggacggaa taatctttag aaagaataaa
      541 aaaatcttaa gataacaact tttttaggaa attttcattt ttcaggagca aaataagaaa
      601 aaatgtgaat agagagaagt gatatacgtg acacaactta aaaaattgtg ccaaaatcgt
      661 ttagcaatcg ttttgacagc tgttttcttt ttctggctga agacgatcat tgcctactat
      721 gcagattttt cattaggtgt ggaaggtacg atccagtact ttatcttgtg gatcaaccca
      781 attgcgacta ccttattgtt ttttggcctt tctttgtata ttaaaaagcc aaagccagcg
      841 ttagctattc ttttgatcat tgatatttta aatacgcttt tgttgtactt gaatattatt
      901 ttttatcggg aattcacaga tttcattaca gtcaaatctg tattaggctt ttcgaaagtg
      961 tcgcaaggat tgtcaggaag ctccttttct ttgatgaagc cccatgatgt catctactgg
     1021 ctggatatcg ctgtgttcat tgggctactg gtttggttga aggtcaagaa aatacctatc
     1081 aaaagcaacc ctgtcggcaa accgatggct atcgctgtta cctgtctttc agggcttatt
     1141 ttttcaggaa atctagcgct gagcgaagcc aaccgtccac aattgctgca gcgaacattt
     1201 gaccgttcat atattgtcaa atatctagga atcgatgcgt atactattta tgacggaatc
     1261 aaaacaggaa tgactagcag tgttcgcgcg catgcaagca gtaatgggat tgatgaagtt
     1321 ttagattata caaagaaaca ttatgcagag ccaaatcccg aaacatttgg gatcgctaaa
     1381 gggaaaaatg tgatcgttct ccatttagaa agtttccagc agtttttgat taatatgaag
     1441 gtagacggac aagaagtcac accgttttta aatagtattt tccagaatca agcaacgatc
     1501 agctttgaca atttctttca tgaagtagga cagggaaaga caagtgatgc tgaaaatatg
     1561 ttggaaactg gcacatttgg tttacctcaa ggctcattat tcacagaact tgggtcagac
     1621 aatgtctttc aagccgcacc agcgatcctt ggacaaaagc aaggctatac aagcgctgtt
     1681 ttccatggga atgtagctag tttttggaat agagatcatg tatataaaaa cttaggttat
     1741 gacaatttct ttgatcgatc ttattttgat gaatccgacg aaacgttagg ttatgggata
     1801 ttagataaag atttattcag agaatctgcc caatatcttg agcatctaca acagccgttt
     1861 tacacgaaat ttctttccgt aaccaaccac acgccttatt atacggatga caagaatttc
     1921 gactttccat ctttgaatac tggcaatagc acagtagata attatgttcg aacggcacat
     1981 tatctggatc aatcattgga acaatttttt acgtatttga aaaaatctgg tatttaccaa
     2041 aattcaatct ttgtgattta cggggatcat tttgggatct cgaatacaga taataaagat
     2101 ttagcaagcg ctcttggtaa agatccagat acatgggatg aatttgacaa cgcacagatg
     2161 caacgagtgc cattgatgat ccatatgcct ggttatacta aagggacagt caatcatgaa
     2221 tacggtggag aaatcgatgt tttgccgaca ttactacatc tattgggaat cgatgataaa
     2281 gattatcttc attttggtac agacttatta tcttctcagc atgatcaagt agttgctttt
     2341 cgaaatggga actttgtgac acccaaatac accgtcctag gaggaaaagc ttacaataat
     2401 cagacaggag aaatcatcga tacgaaagca gcaggaatcg atgaggaaat ctccaaagat
     2461 caggaaaaag tcaagactgc gttaagtctt tcagataagc tgaatcagga aaatctcttg
     2521 cggttttatg tgccaaacgg atttgagacg attgatccga aaaaatatga ctataaaaat
     2581 gaagcggaac gtaatgaagc aatcgaaaag caaaaaggcg aacattcgac aagtgtcttt
     2641 tcaaaaaatg gtaataaaac aacaaccaac ttatatccag taacaagaac agacgttgaa
     2701 cagcaaaaaa ataatcaata aacaatcaaa ccaaccttca gtagttagac aacattctaa
     2761 tgcttgaagg ttggtatttt ttatgaaaaa aacatttttt tatttttttc gtgtattttt
     2821 cctttttaac gtatctattt attgaatgtg gaaaaattta aattaaaaag gagaactatt
     2881 atgtatgatg gtgaattgat caagaaactt cgagtcaaca agaagttgac acagtcccaa
     2941 ttagcggaag gaatctgttt taagacttct ctagtcggga ttgagacaaa ttcagtgaaa
     3001 aaaatgtcat ttgtaacatt gagatcattt ttagagcgaa tgaacatgac acttgcagaa
     3061 tatgaatgga tgcgaaacca gctggatgaa ccaataaagt taaagaaaaa aagacgaatg
     3121 ctagatcggg tacaagaaga cagctttgat ccttataaag agatcagcaa taaccgtagg
     3181 cattttaaga aaacagaaga tctgtattat ttggttttga atcttcacat ggtcgaaaaa
     3241 acagaagtat ctgagacaga atttgaattt ttacgttatg aatgccgaat cattgaagaa
     3301 tacttgatgc agataccaga ctatggtcaa tttgaactat ctattctagc tgattttcct
     3361 tggatattca gtgatcggtt cattgagaac aattatttga aaatcaaaaa aagaatgcga
     3421 cgattagcac agttgaacaa tcatgagcag tatctcttta cttttttaat gaacctgact
     3481 tcgttttata tagaaaatgg tcggttgaaa aaggcacgta gtgtgaatga agatatgaaa
     3541 cggagcttag cacataaaga aagaagtaca gtgatctatg aaacattgat ggcagattat
     3601 tatcaaagat tgatttctgc tgctttaggg gaagaaaaga cgaaagacta ccaagaattc
     3661 ttcacagtgc tcgaatatat gttaggaaag aatcaacgga ggtccttaga gaaaaaactg
     3721 ctggccattg ctgaacccag caactagacc agcagtttag aatttgtatc acttcgccgc
     3781 tttttcagac ggtttgttta tatcgaatgg cgggaaaaag cgggcgatct cttttaccgc
     3841 agcatcacgg gaatcagaag cgtgaatgat gttttcagtc cccggcagcg cgtagtctcc
     3901 tcggatcgta cctggaacag catcggcagc ttttgttgct ccaaccatct tacggacgat
     3961 atcgatcact tcctcgccga ccagcacaag ataaacgaca ggaccggaag tcatgtaatc
     4021 gatcagttca tcaaaaaaac tgcgctcagt caagtgctga taatgttctt ttgctaattc
     4081 aggagtcata gtttcaaact tcatttcagc aatggccagt ccctttgctt caaaacgttg
     4141 gatgatgctt ccgactaaat ggcgtctcac gccgtctggc ttgataataa caagtgttct
     4201 ttccataaca gcacctcgtt tcctactata tttctattat agcgggaggt ggaaaatgag
     4261 acaaataaaa ccgcgtgaga caggaatata acgtgtgtat tttaaaaaaa gtatgaaaaa
     4321 catggaaaat tgatttgaca tcctcttttt tagttgttat attaacgaag gaaaaccgaa
     4381 taagatttgt taggtgaggc tcctatacaa acataggcta ctgcccaaaa acgtcgagag
     4441 acgccaatgg gtagaacagg aattgtcgag ataaggcttt tcataaggta gctaaaagca
     4501 ggtatctgct ttttacgttg tacagtgtca aaactcaacg atgagatctg agaagctttt
     4561 tttaatatgc tttttcaaac ctttcgctga gtatttggcg agaggttttt ttgttgccgc
     4621 aaatccccat cggtgaaaaa taaaaaggag gtggtctaat tggaaaaata actattcgaa
     4681 aaatgattgg aagtcatgaa ttaaaattac ttagtaaagg agatgagaac aaatggatga
     4741 cagtcattcg aaaaacaagg atctagagga aaggccaggg ctattatcta tctgttctca
     4801 gcccctttca gttttttctc tatcgatccg atgaatagct aaagaccaaa agcagcgaga
     4861 acagctgtct tttggacaca aaggagagat tgagatggaa aaaaagaaag atacacaaaa
     4921 aatctatgca gcttttgctg gaaaaacgaa acaagaatta tttgaactat tccatgcatc
     4981 agcaaatgga ttgacagaag aacaaatcga acgttcaaga gaaaattacg gtgaaaatgc
     5041 gatttcatat ggcaaaaaaa caccattcat tgtagaaata ttgaaagcat acatcacgcc
     5101 atttacatta gtgttgatcg cattaggaat tatttcattc atcacggaat atgtattagc
     5161 agctcctgga gataaagatc ttttcggtgt agccattatc ttcactatgg ttatcgtcag
     5221 cgggacaatg acgttgattc agtccgtacg ttccaatcaa gcggctgaaa aactgaaatc
     5281 acttgtgaaa gtaacggcag ctgtaaaacg taatggaaag tacacagaga ttcctatgga
     5341 agaaatcgtc tgtggagatc ttgttcggct ttcagcaggg gatatgatac cagcggatat
     5401 gcgtttattg tccgcaaagg atttattcat ttcccaagca gcattgacag gggaaagcta
     5461 tccagtagaa aagcatgcga aagtatgtga agacgatcag caaagcgaaa caagttacga
     5521 aaatcttgtc tttatgggaa gtaatgtcat cagtggaagt gcggaaggaa tcattgtttc
     5581 tgttggaaat gaaacactat ttggtcatgt agcagaaacg ctatctgaaa aaccaattca
     5641 atcaagcttt gaattaggca tccataaaac atccatgcta ctgatcaaat ttatgtcatt
     5701 gatggctcca gcagttatcg tcatcaatgg gcttacaaaa ggggattggc tagaagcttt
     5761 tctttttgga ctatctgtag cagttggtct aacacctgaa atgcttccaa tgatcgttac
     5821 gacgaatttg gtcaaaggag caagtgtgat ggccaaaaaa ggaacagtga tcaaaaattt
     5881 gaatgccatc cagaactttg gtgcaatcga tgtgttatgt acggataaaa cggggacatt
     5941 gacacaagat aaaatcattc ttgaatatca tatggattgt gaaggaaatg aagatgatcg
     6001 tgttttgcgc catgcgtatc taaatagcta ctatcaaaca gggctgaaaa atttgttaga
     6061 tgtggctatc attgatgaag caacagaaac tttagataca gataagatca attatcaaaa
     6121 agtggatgaa attccttttg attttgaacg tcgtcgtatg agtgttgtgg ttgctgatcc
     6181 agctggtaaa acacaaatga tcacaaaagg tgcgattgaa gaaatgctga acatttctaa
     6241 atttattgat cttggcggta ctgttacacc attaacaaaa gaacgaaaag aagccgtact
     6301 tgcaaaagta cagtcactta atgaagatgg cttacgagtg attggtgtcg cacaaaagac
     6361 caatccaagc gtcgttggag aattttcagt caaagatgag tcagacatgg ttttgattgg
     6421 ttatcttgct ttccttgatc caccaaaaga aacaacgaaa caagcgttga aagctctgaa
     6481 agaacatggt gtagctgtaa aagttctgac tggggacaac gaattagtta cacgttcagt
     6541 gtgccgtcaa gtaggtctag agatcaatga attggttacc ggtgagaaaa tctcggaaat
     6601 gagtgatcat gaattagcac aagtagctga agaccatgaa gtattcgtaa aattgaatcc
     6661 tcagcaaaaa gcacgtttga cgacagcttt acgtaaaaat ggtcatactg tcggattttt
     6721 aggagatggt atcaatgacg ctcctgcaat gaaagtggct gatatcggta tttctgtaga
     6781 tacagctgtg gatattgcga aagaatctgc agacgtgatc cttttagaaa aagatttgac
     6841 gattttggaa agaggccttc tttctggacg agaaactttt gggaatatca tgaaatatat
     6901 caaggctaca gcaagttcta attttggcaa tatgttttcc gtgctgattg caagtacgtt
     6961 cctgccattt ttaccaatgc tgcctttaca acttttgttt ttgaatctga tctatgatgt
     7021 ttcctgcatt tccttgccat gggatcggat ggataaagaa tatttggacg agccaaaaaa
     7081 atggaatgca tctagtattg gggaattcat ggtttacttt ggtccaacaa gttcgatatt
     7141 tgatattact acttacttgt tgatgtattt tgttatttgt cctgctgtag tgggcggaag
     7201 tttccataca ttggatagtt cacagcgagt gattttcatc gcattattcc atgcaggatg
     7261 gtttgtcgaa tcactatgga cacaaacctt agtgctccat gcactgcgta caccgaaagt
     7321 accatttatc caaagcaacg caacatttgc catgtttgtt atcactacat taggaattat
     7381 tgtcggttct gtccttcctt ttacaagttt tggtgctgaa ttaggcttga tgccacttcc
     7441 tggcaattac tggttatggt tatttgctac agtcatcgct tatctggcgc ttgtgacagc
     7501 tgtgaagaaa atctatatcc gtcgattcca agaactctta taaacgagaa ggctggttct
     7561 ttgcttctgt tctttttgga ccagtcgtgc taaactatgt gtattgtttt ggaaaggatt
     7621 aatgtataga tgaaaggtgc aatatttgat ttagatggtt tgttagttga cacagaaaaa
     7681 acgtatcgtg atggctggct ttggggattt cagcaatttg atatccatat cccaaaagaa
     7741 atcgttgacg cttggggcgg aaaaaactgg aaacaaagtt ttgatatttt agtgaaagca
     7801 gcaggtagcc ctgaaaaagt acaggaagtc cgagcgaagc gtgaagaata tttttatcaa
     7861 cagctgacaa gcggagccat ccacttaaaa ccatatgcaa aagaaatatt ggcagactta
     7921 aaagaacgtc atatgaaact aggcattgct acgaccactg ttacgaaaag agcaacagat
     7981 atcttggatc actttaaatt agctgattac ttggacacgt ttacattcgg agacgaagtg
     8041 tcggagaaca agccttcacc gattccttat ctgacagctt tggaacgaac aggattagaa
     8101 gctagttcag cctttgcagt ggaagattct ttagtaggag caacagctgc ttcacgagca
     8161 ggattaggcg ttgttttgat acctgattcc agttttgtcc gaaattatac cgtcgaagaa
     8221 aaggaaaaat tgaaacttct tgcggaagga a
//
LOCUS       DASJST010000118         8203 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJST010000118 DASJST010000000
VERSION     DASJST010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.120"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            84..278
                     /locus_tag="U4B05_002237"
     CDS             84..278
                     /locus_tag="U4B05_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051353.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            547..846
                     /locus_tag="U4B05_002238"
     CDS             547..846
                     /locus_tag="U4B05_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051354.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(943..1377)
                     /locus_tag="U4B05_002239"
     CDS             complement(943..1377)
                     /locus_tag="U4B05_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2051355.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1609..2319)
                     /locus_tag="U4B05_002240"
     CDS             complement(1609..2319)
                     /locus_tag="U4B05_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2051356.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2309..3169)
                     /locus_tag="U4B05_002241"
     CDS             complement(2309..3169)
                     /locus_tag="U4B05_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN2051357.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3174..3812)
                     /locus_tag="U4B05_002242"
     CDS             complement(3174..3812)
                     /locus_tag="U4B05_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN2051358.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3827..4126)
                     /locus_tag="U4B05_002243"
     CDS             complement(3827..4126)
                     /locus_tag="U4B05_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2051359.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4169..5653)
                     /locus_tag="U4B05_002244"
     CDS             complement(4169..5653)
                     /locus_tag="U4B05_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2051360.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5673..6134)
                     /locus_tag="U4B05_002245"
     CDS             complement(5673..6134)
                     /locus_tag="U4B05_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2051361.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6152..7219)
                     /gene="ulaG"
                     /locus_tag="U4B05_002246"
     CDS             complement(6152..7219)
                     /gene="ulaG"
                     /locus_tag="U4B05_002246"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN2051362.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7437..>8203
                     /locus_tag="U4B05_002247"
     CDS             7437..>8203
                     /locus_tag="U4B05_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2051363.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDS"
BASE COUNT         2539 a         1706 c         1321 g         2637 t
ORIGIN      
        1 atcataaaga tatagataca taatcatttt cacttacata tatttgaata ttaactaaaa
       61 acagcaagaa agaagaggat caaatgtatt atgtagaagt aaaaacaaaa ggcgtcaaaa
      121 acaaacaata tgtgaaaggt atcagtaacg aatatccttt acttggttca tggaaagaag
      181 ctgctccttt ttccaaacca tgtgccataa aaataaagaa cgagctagaa aaagaactga
      241 cttgtggtaa agctgttgta gatatcatcg aaaagtaaat ccaatgtata gaaaggaaga
      301 tcacacatga aaagcgacgt acaaattgct aagattatag aattagaaga agaagacgca
      361 gtgttgacaa ttgaaaaaaa ataaatgttt gtataccata tgattatttc aatatgtatc
      421 ctggaattgg agatctcgta gagctatatc aagatgaatc aaatatactt gtcattcatc
      481 atgaaatata gagacttata aaattgttta tttttataat gtatcagtat agaaaggaaa
      541 taaaacatgg attatactgt tgaatttgac agtgaactac ttaagcaatt tccacaagaa
      601 gaacatttca tgtataaaga agcaatttcc cacaaagtga cagagtattt ggagcataat
      661 ttttttcgta taaagccagt gcgtactcgt tcggcaaaag gaatatacga gatgaaagtc
      721 aaaattaata aaaaaaatta tcgcatagca tttgctttaa aaaaaaatca tgtgttcatt
      781 ttttatatta gttcgaattt gcaaaaaatt gtatttgata aagaggtgag taaaaaaata
      841 aaatagctga gcattggagg atagattgag tgatactaag tctatcctct tgtttttcta
      901 aagaactaag ctagaatttt ttcttttgta cgttattata tgttatttgt cattaccaaa
      961 aagcaccttt tttcttctag tatctatgac ataagaaata ccacccattt cctttacttg
     1021 aacaaatcct actgattcat aaaatttttt tgttttctca gtatcatctg tcaataattc
     1081 gatttggact ggtccataac tagtgatctt ctccaaagct tcattcaaaa gcactttgcc
     1141 tattccttgg cgctgatacc tcggatgaat gaggatatct tgaataaatg cgatcgtcgc
     1201 aaaatcagtg atccaacgaa cgattccgat aatcttccct tccccattac gctttatcag
     1261 agattcagat tgttcaaatg ctctagctaa ccttgcagtg tcttttgtgt aattcgtcca
     1321 accaacgctc ttatataatt cagaaatctc atcgatcata agggaatcaa tattcatttt
     1381 tttcttcctt tcttatacaa ttctccctct aataatacag ttctttgact atttaatcta
     1441 tcacatataa ctctcctgta aagattctct cgattcctta aaattcctct ttccttttag
     1501 aatgcatact ttttgataaa taaaaaggtt gtgataaatt ttcttcacaa cctctgtttc
     1561 tctctatcaa gtgttatctt ttaattttct acccttaccc tagcatctct attgcccgta
     1621 gtatgcatac tttccatgtt tgcgaaaata atgtttatct agtaaatatt gcgggatttc
     1681 acatatattg gggttgatca gctcgttttc tttggccatc agacaaattt catctaagat
     1741 caaactgttt tctactgctt tcataggtga atccccccat gtgaacggtc catgtccata
     1801 gacaagtact ccagggactt cgtttggatc taattttctt tccttaaacg tctcgacaat
     1861 cacattccct gtatgaactt cgtaagcttc tcttacttct tctttcgtta attggcgtgt
     1921 acaaggaacc ttaccataaa aagcatcagc atgagtcgtg ccataagctg gcaaatcgcg
     1981 tcctgcttgc gcccacatga cggagtgcgt ggaatgagta tgagtaatag cagttatatc
     2041 ttcaaaagtt tgatacaaaa caacatgtgt ggggagatct gaagatggtt ttaagctatc
     2101 ttcttctatt atatttcccg acaaatctgt taccaccatt tgatctgctt gcatgcattc
     2161 atatctgaca ccgcttggct taatgacgat gactcctagc tcacggtcca cttcactcac
     2221 attgccccaa gtcaatttga ctaatccgta tcgcggcaat gccagatttg cttcaaggac
     2281 acgttgcttc attgctgcaa ttttttcttc acaagacatc atagccagcc tccttcaatt
     2341 tagaacttaa gtagtctttt gcctgctgga tttctcttcg aaagtcttgg cttttttcac
     2401 tccacatttc aatcagaaat gttccatcat aatctagacg tttcaaatgt cttaataatc
     2461 caagaaaatc aacacaaccg ctaccaaaag gaacatcacg aaactggcct gaatattctt
     2521 ttgttacagg gtaagtatct tttaagtgga tagcagtgat ataatcgatc cctttctcaa
     2581 gttctcgagc cggattattt tccggccatg cggacaaatt gcctaggtct ggatatactt
     2641 gtagaaaagg tgaatgaatc tgttctttga tttctaaaaa ttttgaaatg gagttcataa
     2701 atggatcatc catgatttcc acggaaagaa tgatgccata tttagaagct tcctttacgc
     2761 tttcttttat tccttcgata aagtcttctc tggaattcat tgatttctct tcataataaa
     2821 catcgtatcc agctatttgg attactttga cgcttagatt atgcgccaaa tggattgctt
     2881 ttttcattag ttgtttcgct gcttttttct tttctggatc agcagaacca aacggaaatc
     2941 gccgatgcgc acttaaacaa atcgaatgga tacgtacacc ggagctgaac atctcttctc
     3001 tcagctcagc tatcttttct tcagaccaat ctaatcgtgc taaccgctca tccgtctcat
     3061 caatcgacat ctcgataaaa tcgaagtcca tttcttttac aagggaaaaa cgttctttcc
     3121 atgaaatatc tttgggtaac gctttttcat aaatcccaat ttgagccatt tccttacccc
     3181 cagatacgaa tgatctcatc tttgaagttt tgtgcggcct tcatcggatc atttgctgct
     3241 gtaataccac gaccagtgat aaaggtatac acatcgatcc cttcaaataa gtgtaatgtg
     3301 tgtggattta atccaccagt tacagaaaca ttaaagccta gttcaatcag ttttttgatc
     3361 ttcgataaat ctttttcacc ccaagtttca ccagataaca gcgcatcacg gctttgatgg
     3421 tagattacct gccggatacc gatgttatgc caatccatgg cttgttcaaa agtccaattg
     3481 ccgtagagtt ctacttgcaa ttcacctatt tctttttgtg ctgcctccat agttggaatc
     3541 gttgcacaac aaatgactgt catccagtct gctcctgctt ttgcaacatt actcgccact
     3601 gttcctccag cgtctgcaca tttcgtatca gcgactagtt ttttatctgg gaacatactg
     3661 cgaatacagc gaatcgcttt ttgtccttcc tgtaagcaaa gaattgtgcc cacttcaata
     3721 atatctacta catcaccaac tttcataaca tctcctaatg catgttctaa gtcattgtga
     3781 tctaacgcaa tttgcaaatt cggcctgctc atcttagttc cttcctttac ttgttttttt
     3841 ggtaccactt tgtttcagca aatttttcac tgatttcttt gactgacatc acatttttga
     3901 taccgataac tgtaactcct tttttctctg cctgagtaaa catattcaaa aaattgactg
     3961 gcgtaaaaac tacatcgtat tggccagctg aactttttcc ctctgaaatg gcacaatgat
     4021 ggattttggt aattggtacg tttagttctt tcattgcttt ttccacactt ctcatcatca
     4081 tcaagcttgt tcctgatcca tttgcacacg atactaaaat tctcatctta atccctccat
     4141 tatgagtgtt gtctaatcag attttctttt atactctctg cttttctgca tatgtgtcat
     4201 agtcttctac aatcaaaaaa taaccatcag gatttttccg atattgtaac tgcggaatag
     4261 ccaataaacc aatgacaact acggcaattc cagcataacc taggactttc atgattactg
     4321 taaacaatgg ccaagctgtt gcccagtcga acatcccgat ataaccaccg tatgttgaca
     4381 tcccaatcca tgtagcgaat aatgctgaac cgcctacttg tatgattcct gaaagaaacg
     4441 ggaacaggca agcggctttg aatccacctc ggttattggc ataaactgct ataacggcat
     4501 tatcaaagaa caacggaatg aaaccggcta tgacgatcgt tggtgatttc aataaaatca
     4561 gtaaaccaat catcaaaaac tgtcccaaag caccaaacaa gaacccaatc gttaccgcat
     4621 ttggcgaacc aaagccaaaa gtagcagcta cgtcaattcc tggaacagct cctggtaaaa
     4681 tcgtgtttga gattccttgg aaagattgag ttagttcatc gacgaatgtc cgaacaccta
     4741 attgcaagat cgccagatat accgcaaaat ttaaagaagt cgtcataatg tagaataaga
     4801 aactctgccc ttcttgcatg aattgcgctt gaatcaaata atctttgcct agtacaatca
     4861 gaatgatccc aaagaaaaat agcatgagta acgaagtagc aaccatattc tcattgaaaa
     4921 tcgacaagaa tcctggtaat ttgatatctt caattttttt gtcttgcttg attgatctat
     4981 ttttcttttc atcccgcttt ttcatccact cagctaattt tgcaaatata aagattccga
     5041 acatttgctg atgtgccact gcaaatccgg caccttctgt caattcctgc gtgattccta
     5101 ctgttaagtt agaagctact gtccaataac aacctaaaat cagtcccata aaaatcaatg
     5161 cttgtatcct acctagatca ggaaagcaaa acagtaaaat ccaaaaagcg gtagctgctt
     5221 gttgaacctg aacgtttccg gtagtgaaaa cggaacgcag tttcgtatat ttctgaaaac
     5281 ggactaataa gatatttaaa acaaatgcaa tcagtaataa aatcatcaca tctccaaagg
     5341 tacggccaaa tgtctccatt aatccagcat ccacagcatt ttgtccaaaa taaggatctg
     5401 taaccatggc atccaaattg aaccgttcct ttagtccaac caagattggc cgaaaattat
     5461 tgaccaaacc accagagcca acagtcaaaa taaagtaacc gaccgttgcc tttaagaatc
     5521 cagcgatgca ttcatgaagc gggcgtttca gcaaaatata gcctaataac acgataaaac
     5581 caatcaggta tgctggctgc gtcaagatat ttgttgcaaa atacgtccaa atgcttaaca
     5641 aaaattcccc catctttttt ctcctctatc aattaattat attctgccat cacaacttgg
     5701 taatcatcga tggagttcgt agagatgaga cgctcaacca atccatctgt cattaataat
     5761 tcggataatt tttggatatt ttctagatgt tcttctggat cttttgctgc gagtgtaaaa
     5821 aacaaactag ccgttttctc ctcattctcc ccttcacttt caaaacggat tggctgtttc
     5881 aacttggtaa atgaaatagc tgttccaaaa acaccatcac ttttttctga agaatgcggc
     5941 attgctactt gaggtgcgat cacaatataa ggaccgtatt tttctacaca atggatgatc
     6001 tcctccacat acgtttcatc aataatttcc ttatcgatca atacaagaca gctttcggcg
     6061 atagcttcac gccaatccgt gatttcttta tctgggtaat gaatcaattg atggtcaaaa
     6121 aagtatttca gcaagaaact ccccttcttt cttataaaaa cgacggaaac ggtgtatcat
     6181 tttcaatcga gaagacatct tcaaatcctc gataaaaatt aaactcaaga tcatctttgt
     6241 ttgtcggata gatgaatttc ccgcctactt gccagatata aggtttaaat ccatacttca
     6301 ggcgatcctt tttgaatttc caaatttcag tgatctcttt tggatcagcc ataaaattag
     6361 accaaatatc gtagtggaca ggaatcacta cttttgcatt caatgactcc gccatgcgca
     6421 gcatatcaac agaagtaact ttgtctgtta tccctcgtgg gttttcacca taggccccta
     6481 aacagacatc gattttgtgt tcattaccat gtttggcaaa taaattggag taatgtgaat
     6541 ctcctgcatg ataaagattc cctcctgatg tttcgaataa atagtttact gcaatttgat
     6601 ccatctcctg aggcattttt ccttttaata cctcttctgg atcttcacaa gtgaccaacg
     6661 ctgtccgatc aaacgcttct aaagctacta cattgatgtc tttgattttg actgcatcac
     6721 ctggctttac tgtaatcgtt ttttcttcag gaattcccca tttttgccac gtgcgaacca
     6781 cttcttttgg cccaatgaat gttgcttcag gacaattttg atgaactgct gcggctgtat
     6841 ggatatctag atgatcggaa tggatatgtg taacgattaa agcgtctaca ttttttatcg
     6901 caaaaggatc gattacgaaa ggctgagtac gtaaattagg ctgcatattt tgaacgccac
     6961 tcatacgcat catctgatgg ccttttttca tcatgccatt cccatgacta cgttttcccg
     7021 tcccacacca tagatcacat aaaatattcg ttccttcatg acttttcagc caaattcccg
     7081 tacagcccag ccaccacata gcaaaagttc caggatccac tacttctgtt tcgatttctt
     7141 cattcaagta tgtgccccac tcaggaaatg tttctactaa ccacttttct tttgttactt
     7201 cattgacagt tttactcatt tgcttgctcc ccccatatcg ttgttccaac ttacaagtaa
     7261 atattaatgt gtatcagcat ccatgaaaac ccttttatat gaccgtttgt tttttatatg
     7321 attttctttc agtaatcaat gattgtttca gtataataaa gacagatatg aatgtttaaa
     7381 tgaacaacta gtcaaataaa tccatggatc aagatgtccc tcaggaggca aaagtcatga
     7441 aaaactcact tgtcaatatt gaaaaacgtc aaaaagaaat tttggaactg cttcagcaga
     7501 ctgaacaact atcaactttc gaattagctg aatcccttga tgtatctatt agtacgatac
     7561 gaagagattt aaatttatta gaagaaaaaa acgagatcat acgcaattac ggttattgtt
     7621 cttataatta caaaaaccag actgattttg atcaatctgg tcctgaaagg attaaacaag
     7681 cgattgccag agaagctagt aaatacatta acgattatga tacgctgttt atcaattcta
     7741 gctcgactgc gttgaaaaca ctggattttc ttcaatctaa ccatttgaca gtcattacaa
     7801 acaacgtgaa aatcaattac tctccgcaca aagataattg tagttatatt ttaactggtg
     7861 gagagatgcg ctttccaaaa gaagcactag tgggcgacat cgcattaaat acgatttctt
     7921 cagtcaatgc agacatttgt atcattggct gtagcggagt cgatgtagta catggggtca
     7981 caacaaagat attaaatgaa tcaacgatca acgaaatgat ggtcaacaag acaatcaaat
     8041 gtaagatttt agtcgctgat catcgaaaaa tcggactgac atcaaagttc aagattgctg
     8101 atatttcagt ctttgattat ttgatcactg ataaattttg ttctgaaaaa gtattagagg
     8161 agatcagaaa tacggggata aaaaccattc aagtagattc att
//
LOCUS       DASJST010000119         8165 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJST010000119 DASJST010000000
VERSION     DASJST010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.121"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..809
                     /locus_tag="U4B05_002248"
     CDS             108..809
                     /locus_tag="U4B05_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2051364.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1418..2434)
                     /gene="tsaD"
                     /locus_tag="U4B05_002249"
     CDS             complement(1418..2434)
                     /gene="tsaD"
                     /locus_tag="U4B05_002249"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN2051365.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2431..2895)
                     /gene="rimI"
                     /locus_tag="U4B05_002250"
     CDS             complement(2431..2895)
                     /gene="rimI"
                     /locus_tag="U4B05_002250"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2051366.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2902..3444)
                     /gene="rimI"
                     /locus_tag="U4B05_002251"
     CDS             complement(2902..3444)
                     /gene="rimI"
                     /locus_tag="U4B05_002251"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2051367.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3428..4150)
                     /gene="tsaB"
                     /locus_tag="U4B05_002252"
     CDS             complement(3428..4150)
                     /gene="tsaB"
                     /locus_tag="U4B05_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN2051368.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4341..5321)
                     /locus_tag="U4B05_002253"
     CDS             complement(4341..5321)
                     /locus_tag="U4B05_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2051369.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5345..6829)
                     /gene="galT"
                     /locus_tag="U4B05_002254"
     CDS             complement(5345..6829)
                     /gene="galT"
                     /locus_tag="U4B05_002254"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN2051370.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6841..7830)
                     /gene="galE"
                     /locus_tag="U4B05_002255"
     CDS             complement(6841..7830)
                     /gene="galE"
                     /locus_tag="U4B05_002255"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN2051371.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8078..>8165
                     /locus_tag="U4B05_002256"
     CDS             8078..>8165
                     /locus_tag="U4B05_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN2051372.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKT"
BASE COUNT         2312 a         1731 c         1445 g         2677 t
ORIGIN      
        1 tttgtaaagt attaaaactt gtttctaatg ttggtatgtt aaaactttac aaattcttta
       61 aaaaaagtga caataaatat agtgaacaat aaggagagga ctttttaatg cgtgttaata
      121 aaatcgattt tcacttttca aaactcagat atcaacctga ttttgaacat ttctcggatg
      181 aagagttcca acagatcaag gaacatactg ttcttcgttc ttataaaaaa ggacagatcc
      241 ttttcgacga tggtgatccc agacaacggt tgtatatctt gaccaaagga ctcgttcgtt
      301 tagaacgtta tgatgaaagt gcaacttatt actactatga ctatgtgaat acaaataacc
      361 tttttcctat gtcaggaatg tttggagaag agacctacac ttatacagct caagcgatga
      421 ctgatattga aacattctac cttcctgttt cctactatga aaaattagca cgaaaaaatc
      481 ccgaacagtt gatctatatg atacaaaaaa tttctaaagt agttgaaata catgaattgc
      541 gtatccaaat cggattgacc tctagcgcct ttgaccgggt aaaacaaaat ttattcattt
      601 tgaaagaaga attgggcgta ccaacaaaag atggtcatat caccattcct tatccgatca
      661 ccttaaaaga attagcagtg aatagcggca cgacacgtga aactgctgga caagtaatca
      721 aacaactacg tgattccggg ctgttagatt atgacaaaaa gcgttttact ttctgtaaga
      781 cagctactac tccagctatg agagaataac gcgaacaaaa ataagcagtg accctgataa
      841 atatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg cagttttaga
      901 tcactggaca agactcaaag attttctatg tgtgtttcag ttatgtaata ttcactgttt
      961 gcaaaaaata ctgttagatt aactagatga agcgtcttgt cctcatccga tctagattat
     1021 tcgatattcc tcgacaataa acgattactc agtgagggca atcagaggaa cggataatga
     1081 taaataagct gtaaaaaata gcaaaataac aataatgatt aaaagtatgt aaaaaatagg
     1141 gctttgacaa aaacgtgtca cagccctatt tcgattaaac ggtgtgtacc tgtcaactct
     1201 tcggtcacaa gtcataatgt tcataaacat gaggagctgt tttttgaaat tgttccttgt
     1261 gaatcagagt taaacgacag gcttacgaac tcttgattaa acggcgttca aaagcttgca
     1321 tacgcggtaa taagaacagc caggtcaaaa acatgagaag ccgtttttcc tgggctgttc
     1381 ttattacttg atgcagggca gctttttcac aacctcttta ttcagataaa accaagcttg
     1441 gatcagcatt caactgatag ctagcaaaat ttcttttttg catttccaca tgtgctgctg
     1501 ctccaatcat tgcagcatta tctccacaaa gtcgaagagg gggaatcact agttctacct
     1561 cagggagttt agctgccaaa gcatcctgca atccttcacg caacccttga ttggccgcta
     1621 cacctccagc tataatcagt tgcttgactg gataattttg acaagctcga agtgttttag
     1681 taatcaatac atcgatcaca cttgcttgaa agctagctgc taaatcattt ttgtctagat
     1741 cttctccacg ctgttgtgca ttatgcacca aattgataaa tgcacttttt aatccgctaa
     1801 aactgaaatc ataattatct tcatggatca tcgctcgcgg aaaatggtaa ttatcttttc
     1861 cctgatgagc tagctgatcg atttcttttc cgcttgggta agaaagacct aatactcttc
     1921 caactttgtc ataagcttct cctgctgcgt catctcttgt ctccccaatg atttcatatg
     1981 atccatcttc ttgcatatag actaattctg tatgtcctcc gctaacaaga agtgccatca
     2041 atggaaactg gaatggttta accagacggg ctgcatagat atgccctgcc atatggttta
     2101 caggaataag cggcaattgg tgtgcccaag caaaagcctt agctgcagaa ataccaatca
     2161 gcaacgagcc gacaagtcct ggtccataag tgacagcgac tgccgataaa tcttctgccg
     2221 acacaccagc ttctaccagt gcatcttcca agcataaggt gatttgttct acgtgatggc
     2281 ggctagctac ttcaggtact acgccaccaa aacgtttatg actgtttatt tgagaagcaa
     2341 cgatgttaga taagatctct gttccatttc taacgaccgc tacactcgtt tcatcacaac
     2401 ttgattcaat cgccagaatc aattcttcat tcatttcatc gtctccgttt ttaactttct
     2461 gctcataatg atcgcatctt ccgtcggatt gcgataatat gatttccgtt tacctaatat
     2521 acgaaacttt tctgattcat aaagttttct tgctgcttca ttagatagac gtacttctaa
     2581 aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc
     2641 atgccctttt ccttgttctg aaactgcaac agcaatattt gtgatctcga cttcatctat
     2701 tactttcgag tatccaataa agccacagat ctggttatct tcagctaaaa gcagataatc
     2761 agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg
     2821 ataagcattt tcgctaatca tccatacgca ttctgccaag tacgcttcat caaaatcttt
     2881 ttttctgcaa atcaactaat ctcactcatt ttctaaaagt tttatcatat ccaacgcatc
     2941 ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt gcttgaaacc ccaatttccg
     3001 ataaagtctt tgtgcatctt gattactcat acgcacttct aatgacatcg tctcacagcg
     3061 attcattata gcaaagttct cgatttcgtc aataagcagc gaaccgattc gtttacgctg
     3121 atatgccggt cgaacggcaa tattcgtgat atggcaatct aaaccgaacg ctcttgaacc
     3181 gataaaacca atcattcttc cattatcaaa gatacctaga tatagatgag gaagcggtga
     3241 cttcaattct gatataaagg cactttttgt ccatggcaat tcgccattat agacttctcg
     3301 ttcgacatcc agcagctctt tgatatcttc tgtagtcaat tctcttagta cgtaacgacg
     3361 atcgatttgt tcgactgttt tttctgtata aatcatttta tcttgaaaaa agcttctggc
     3421 atacgctcta aattttttca acataacttt cgtctccagg tgtgtgcgtc tccaaccatt
     3481 tttcttctgc ttctactcgt tttaaatagc gaggaagaaa agcttgtttc tctgcaactg
     3541 gttcaactaa agcacctaat tgagccacag ttacaccact tggaatatcc catgctggaa
     3601 ctgtattaat catcgcacta ggcaatattt cctttatttc agagaagaat ttgtgacaat
     3661 cactcccgac gaaataaata gcttcattca gcttttgtag ttgtgtgaac aaatcagaag
     3721 cagaaatatg tacatcttca agtacagttt ctaatctatt ttctttccag cggtatgccc
     3781 cagcgtatac attttttctt cgagcatcaa acatgggcac aataatatta tccacaccga
     3841 tgcaattagc cgcgatggtt ttcagactag atactcctac tagctcttta tctaatgtat
     3901 aagcaatcgt ttttgctgtc gttacaccta tacgaagccc tgtataagaa ccgggaccat
     3961 cagaaaccgc gattcggtca atgtcttttg gacttatttg aagatctgca aacaattggt
     4021 cgattgcagg catcagtgtt acactgtggt tgcgtttgat atttgtttgt atttgtccaa
     4081 gaatttgttc gtcttctacc acaccgatcg ccaaagtttg gtttgcagta tctattccta
     4141 atgtaatcat atatttgatc ctttctgcta tgacattctc gccttccatt gtagcatact
     4201 tttttattct gaggatgaaa ataccaaagg caaaactagt acaataatac atgattatct
     4261 gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa gctgcggtat cactccattt
     4321 tcccacagct tttccatctt ttatttcgtt gattctcgaa taatcaactc tgtttccaca
     4381 ataatctttc ttgaaaccgg tgctttggaa ttacttaatt gaagaatcgt ctccactgct
     4441 agctctccca tccattcggt ataaactttt acagtagaaa gcgagggcgt tacatatttc
     4501 gctacactga catcattgaa gcccatcaca gaaatatctt ctggtaccac taaacctttt
     4561 tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat tagctgcaaa aagcgcatca
     4621 ggaacatgcg gattcgtttt taaatattgc ttcactgctt gatatcctga ctcgacagaa
     4681 aaatctgctt cgataattgg accttcgtcg tagccttttt ctgccaaaac ttgttgaaat
     4741 gccataattc tgggatcttt tagccgctga tggtttttct tcgtatattc tgttccagca
     4801 agcatagcga tatgttgcgc tttatgggag acaaagtgtt caatgacttt ttgaacactt
     4861 gacttaaagt cgacaactag agagtcgatt ccttgagaag tgccgtcaga atctaccaaa
     4921 agcaaggcat tttgggatcc tttcatttct aataactgag cttcgtcgaa ttttcctagt
     4981 gcgatcgttc catctgccgg aagatcacta atggaatccc acgtttcttt caataaagta
     5041 acgtttgctt cttctgcctt tttttcgatt cctaggcgaa tagataaata ataaagatct
     5101 tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa gcgtttgctt agtatttgct
     5161 gcatttttac gatgtttcgt ataatttaag cgttctgctg cttcaaaaat tttttgcttt
     5221 gtggctaagc caacggaaag atcaggatca tagttgagaa ctcttgaaac agtagcagga
     5281 gaaactcctg ccaactgcgc aatgtcttta attgttgcca tttgtctctc ctcctttttt
     5341 acgtttataa aatcgcagta aatcgttcaa acgctgcctg tcctttttca tctcgcttga
     5401 atactccagc gtcttccagt acacgtgcaa aaacctgacc tacagatttt tgtacgacat
     5461 cggtgaccgt atctttcgtc aaatcaggat gttctttttt taattgttct gcccacggac
     5521 gatgatattc agccatctgg ttttcttctc ctaataagaa atgctctact tctactagtt
     5581 ctggtttcaa acgaggtggt aaaacagcaa gccccattac ttcgatcaac ccaatgttct
     5641 cctttttgat atgttggaca tctttatgtg gatgaaaaat cccatcagga tgttcttctg
     5701 aaacattgtt gtctcgaagg acaaggtcca actcaaagta attgtccctt cttcgtgcaa
     5761 ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa agctctgata gatactgttt
     5821 catccgaata tgctttccat ttttccaata ctgagttagc tgcttcaata agcaagtttt
     5881 tctctgttga ttgcaaacgg atcacagaca tcggccattt cacgactcct gcgatgactt
     5941 gcggataatc ttctagtttg ataagttttt ctatcggtgc ttttgccata ggaaattcat
     6001 gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc tccaacaatt ggtaagtcag
     6061 cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat ccgaagcagt ctagcaaatg
     6121 tttcctttgt gattttcatc gggcggtgtt gttctgataa aatgatcgaa tgttcattgt
     6181 aataagcata aggggaatat tggaatcccc agctttcacc gtctagattc attcgaataa
     6241 tccggtgatt ggtgcgggct ggatagttca agcgacctct atatccttcg ttttccatgc
     6301 aaagcatgca ggtaggatag tctactttta ccgcctcgcg ttctgctgcg atttgttttg
     6361 gatctttttc tggcttagat aaattgattg tgatttccag ttcaccatac tccgactctg
     6421 ccggaaattt gatgtttttt gcgatggctc ttgttttgat gtagtcattt ttcttactta
     6481 aattaaagaa atactcagtt gcttcctctg ggcttttcga atagtgctgt gcaaagaatg
     6541 cattgacgac tgaaggtggc ggtgtgagaa aatccattaa ttgcgcttca aaaatctctt
     6601 gttctgctgg caattctgaa agaacaccat tttctcttgc cttagcgaca agctgatcca
     6661 ataagtctac agattcttcc ttaactggtc ttacttcaat gctttctaga gcatcttctc
     6721 ctaccatccc taaaagacga ttctgtaaat ataagcggtc catttccatc catccgccag
     6781 cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact gattgacata tctcattccc
     6841 ctattcttca taaccatttg gatggctgac atgccaatcc catgctgttt tgataatcgc
     6901 tttgatatct gtgtattcag gtttccagcc cagaatctct ctcgcttttt cacttgaagc
     6961 gactaatcgg ctaggatctc cagctcttct tggtgctatt tctgctggaa tttcttttcc
     7021 tgtcacttct ctagctgctt ctagcatttc cttgactgag tatcctttgc tgctgcctaa
     7081 attgaagaag ttgctttcat ttccttcttt caagtattcc aaagctaaaa tatgtgcagc
     7141 aatcaaatct tctacttgaa cataatctct gatacatgtt ccatctggtg tatcataatc
     7201 gtctccataa acagccaagg cttttctttg accaagagct acttgtaaaa tgattgggac
     7261 aagatgcgtt tctggtgtat gatcttctcc gatagatgca tctgcttttg ctccggctac
     7321 attgaagtag cgcaaagcga catagcgcat gccatatgct tggtcacacc acttcatcat
     7381 tttttccatc atcaatttac tttcaccata cggattttta ggattagctg gtgtatcttc
     7441 tgtgattgga gactctttcg gctctccata agttgctgca gtagaagaga atacgatttt
     7501 attcacgttg aactcgtgca tgacttcaag taagatttgc ataccgtata cgttgttgtt
     7561 gaaatacatc aatggttttt cgacagattc tcctactaaa gagcttgcag caaaatggat
     7621 tactccttca atcggttcct tttcaaatac ggaacgtaga aattctttat cccggatgtc
     7681 tccttcataa aaatgagcat ctggatgtac tgcttgtttg tgacctgtga gcaaattatc
     7741 taccacagct acttgatacc ccttttgaac tagctggtct acagcgtgcg aaccgatata
     7801 accggctccg cctagtacta aaattgtcat aaaaaacact cctttgatta gtaaaattgt
     7861 gtttgttgat tctatataat ttaaaaaacg aagtttactt ttatggtgaa aacaaacaaa
     7921 gtaaaatttc gtaaattcat atggtaaatt tataataaaa catttagtaa acattttcaa
     7981 gggttttttg caaaggttta caaacaaacg taaaactagc ctttttttct cttttgttcc
     8041 ataatagata taacaaataa taaatgaggt ggattcaatg aaaaaatttg tatctggaat
     8101 attagtagga agtctggcaa cagccgctgc cgttgctggt ttggttgctt ctgtgaaaaa
     8161 aacag
//
LOCUS       DASJST010000120         8133 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJST010000120 DASJST010000000
VERSION     DASJST010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.122"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(184..1950)
                     /locus_tag="U4B05_002257"
     CDS             complement(184..1950)
                     /locus_tag="U4B05_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051373.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1910..3664)
                     /locus_tag="U4B05_002258"
     CDS             complement(1910..3664)
                     /locus_tag="U4B05_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051374.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3774..4343)
                     /locus_tag="U4B05_002259"
     CDS             complement(3774..4343)
                     /locus_tag="U4B05_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN2051375.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4361..5776)
                     /locus_tag="U4B05_002260"
     CDS             complement(4361..5776)
                     /locus_tag="U4B05_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2051376.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5787..6455)
                     /locus_tag="U4B05_002261"
     CDS             complement(5787..6455)
                     /locus_tag="U4B05_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2051377.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6586..7170)
                     /locus_tag="U4B05_002262"
     CDS             complement(6586..7170)
                     /locus_tag="U4B05_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2051378.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7505..7735)
                     /locus_tag="U4B05_002263"
     CDS             complement(7505..7735)
                     /locus_tag="U4B05_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN2051379.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(7750..>8133)
                     /locus_tag="U4B05_002264"
     CDS             complement(7750..>8133)
                     /locus_tag="U4B05_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2051380.1"
                     /translation="LDKEAKSKFASIPNDKKMIVTSEGCFKYFSKAYNVPSAYIWEIN
                     TEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNIPIYSTIFTDSIAE
                     KGQDGDSYYAMMKWNLDKIAEGLSK"
BASE COUNT         2734 a         1474 c         1076 g         2849 t
ORIGIN      
        1 tcgccaattt tttaaaactt tgattattcc ttatattata agaatagaca ttaagtgaac
       61 taattcaatc atactgattc tttcgtgtat tcaaaattag atgctgttta ataaaattat
      121 tctttttgaa aaagaattat aactatatcc caaaaattgt aggttaattt aaagcctatt
      181 tcatcatcgt gattgagctt ctttttcata gacgaatttc tcagatagtt tagttttctt
      241 tatgagattt ttgtatattt ctgaccttcg atacaactca ctatgagtac cttgatcaac
      301 tatttttcct tgatccatca ctacaatttt atttaccttt tcaatagact tcaagcgatg
      361 cgatataatt agtactgtct tatcttgtat taaacgattt aaactttcct gaatacgttt
      421 ttcattttcc gcatctaaac tggcagtaat ttcatctaaa attaaaattg gcgcattttt
      481 caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct ccagaaagtg aacttccgtt
      541 ttctccgacc aatgtatgat aaccgtcagg tagtttttca ataaagtctt cgcagttagc
      601 taaacgagct gcttttttca cttcctcatc tgacgcttga ctatttccaa tacgtatatt
      661 ttccaaaata cttgtattaa acaaagttac ctcttgaaaa acaatagcga ttttgctaaa
      721 caaagaagct ggagatacac gtttaatatc atacccatca attaaaatac agccttcatc
      781 ataatcatac agtctagaca ctaatttcaa gatactagtt ttcccggaac cactagcacc
      841 tactaacgca gtcacttctc cttgtttagc cgtaaaagag atatgatcta aaattggggt
      901 attgttatca tatgagaaag agacatctcg caactcaatg tcaaaagatt tcagaggact
      961 atctgatccc tcttggatac tggtttcctt catagctcta attcgctgta ttttaggagc
     1021 taaataaaat atttcaagca ctgcttcttt catagaatcg aaggaatcct ttatttttat
     1081 tgcggctagt aagtaaccaa cgacgtagag tatagtcact tcacccgtta acagtaagtg
     1141 aacacctact agaatgacta ctgctaaact aacatatgag aaaatagagg ataaggccat
     1201 gactgaaaaa gtacttaatt ctacctttaa atgaatcctt tcactctctt ccattttttt
     1261 gaaaagtctg tcttgaactt ttttagatag attaaagcta ttaatctctt gctgcaattc
     1321 tatagtttct tgaaattctt ccgagttttc tctcaaagta tcgtaatatt tagtattggc
     1381 tttcgtttgg gatttctttg ataacaagat taagacaaaa ctaaataacg ttggcaaaat
     1441 aacagctaat cccattttga cattaccaac caaaagcatc actgaaataa aagggaaaaa
     1501 cagagccata ccgccggctt taggtattgc atgactcatc gcatgctcaa taccttcaac
     1561 gtcagacatg atagtttgag ataaatctga taaattatgt tttgaaaaat aggataatgg
     1621 taaatttgac aaatcgtctg caatttgcat tcttaaatgc gcactttctt tataagtact
     1681 attatataag ttttcatact ccctatctaa caaccaatac atgaaaagca aagtaacgaa
     1741 cgatactgta aaataaagcc aaggcggctt tacattttct aaaactaatt cgtctatcag
     1801 catcattata atgaatgctg gtaccatatt aatgcagtat acaaaaaagc tagaaataga
     1861 tgctttaacc aaatcttctg cgccctgatt agttaaagcg aatttgtttt tataaaaact
     1921 tttcattgtc aagcctccaa tcatttgcat tctcataatt agtgaccaat tgtttataca
     1981 cttgatgttt agccattaat tcattatgat ttccacgttc tactattttc ccttctgaca
     2041 agacaagaat ttcatctaca gatttaatgc tggataatcg atgagctatc ataataactg
     2101 ttttttcttt catcagatgt tgaaaagcct tttgtagttc atactcatta tctggatcaa
     2161 tagatgcact ggcttcatcc ataataacaa tctttgcatc ttttaaaatt gctctggcaa
     2221 tagctatacg ttgtttttct cctcctgaca gataaactcc tttactacca attaaggtat
     2281 gctctctttc gggaaatttt tctaaaatcg attcgcaacc ggctaatctt agcgcgtcaa
     2341 atactttact tgctggcgct tgaggatttg ctatagaaac gttatcgtaa atactctgat
     2401 tgaatagttt cgtatcttga aagacaaaag caattgtgtt aataattgtg tcttttgaat
     2461 agtgatttaa aggcttacca ccaattttaa tctgtccctt atccacattg tagaaacctg
     2521 aaattagttt agctatcgta ctctttccgc ttcctgattt tcctactaat gcataaattc
     2581 ttttttgttc taaaactaag ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc
     2641 tgacattttc aaatgcaatg tcacaatttt taatctgtgt ctcgttccca tattttagtt
     2701 ggtcttgctt catttctaaa taaagggagt ctagtgtttc gactgcatat cttgcttcaa
     2761 acaagtgcat acttacatac attattctca tgaaagaaac aaaaataatt ccacttaaaa
     2821 aaaacaacat caacaagtca acagctaata tcttgggagt ggtaagagaa gttataaaaa
     2881 aggttatagg aactatcaaa atgcaaacta tgccaaaaaa tagccattga aaaagaacaa
     2941 aaggtgtttt cccactcaaa gaatacttat atgcaaactt agcataatcg ttaattgctt
     3001 tattcaaggc tttaaatgat gttactttag caccaaatat ctttataact tgaattcctc
     3061 ggatatattc taccgtttca gagcttaaca catctaacga ttcttgatat ttttgaataa
     3121 aatttgtatt ctctgtcatt ttctttaaca agaatacacc aattactaac atggcgatta
     3181 gaaccaatcc aattttaaaa ttaatgaaaa agcctaaggc aactgataaa acaggtgtta
     3241 ctatggcttg agcactatct ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt
     3301 tgtcatcaat tgtctttcga atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg
     3361 cttctgttaa accgctaatt cccttttttc tcaaattagt ctctaagcga aacccaagga
     3421 tatgagaaaa taagccagaa aaaatataaa ataaagctcc taaagttaac caagcaacag
     3481 tttgaaccgc ataactctta gctaaatatt catctcctac aataattaga gacttaagaa
     3541 atttgtaaat actgtaatat ccatagactg ttaatacgac agaaaaatca gaaaataaag
     3601 tggctaacca tccgtagtat ttcattttag ggacataact aaataatttt ttgtatactt
     3661 tcattttttc ctcccccgca ttcaatttct tctcttattc gtttacaact gttaatgctt
     3721 tccgtaatag cgtaaattag agatgtacaa acagatagaa ggtaaactac gttctatttt
     3781 ttgtattttt ttaagacttt tcttccaatc aatacaccaa tcatagataa acttgctaca
     3841 actaacactg aaatgattcc ccatatacct accgcatagt ggatcattgc atccattgtt
     3901 gcagcagatt gtcctttatc tatccaatct tgacgaaaac gatctgtaaa gaaccatata
     3961 ggaatccact gtccggcagc actgcctaat tgcattagag cccaagacgc aacttgagga
     4021 attgctcgat catatccaaa tacagataga ataatatcag ctaagatcgc catactaatc
     4081 gaactgataa accaaggtaa gtacgccccg cccattaacg taaaaaataa caataaaaca
     4141 attgtgtaaa tagtaaaaat tccttttcta gggtagttat agctaatata aacaaatatg
     4201 attccagata ataatccaaa aatccctggg gatatgctgt gaccaaacgc cccaccaata
     4261 ctactaacca ttattgaaaa aaagtaaatt actagggaaa taactgtagc taaaccaata
     4321 aaaatataat cttttgtttt catatatatt ctcctcaatt ctatttcgtt atgtcattaa
     4381 aaatctgttc tatcaaagtt gaatcttcta ctctaagttt tatatctttt tttattccac
     4441 cattttttaa ataaataatt ctattgcatg ttttataaag aaattccgga tcatgagtgg
     4501 ctataactac tgcacccttt tcttttaagt ctttaataag gtcagcaatc ctttccatat
     4561 taatataatc caacccactt gtaggttcat caaatagaaa taaattagtt tttgacaatg
     4621 aagctataga aaccaataat ctttgttttt ctccaccgga taaatcaaac ggtaattgat
     4681 ctttcatttt ataaagttcc atttttttta aaattttttc ttgctgatct ttactaacta
     4741 tttcattccc cagggctaac tcgctcctca ctgattctgt gaaaaattgg taatccattt
     4801 cttgcattac taaaaacggc gatgacaaat tttttttatt ttcgattatc tttcctttgg
     4861 tagggcgaat aatcttcatg agtattctta gtaaggttgt tttccccaca ccgtttttcc
     4921 ctaccaaacc aataacatct ccaactttga catcaaaatt tatgtctttt agaatatttt
     4981 cataagtaat ttcttcaact tttaatacat ctgcacttac cttttcgata tagggaactt
     5041 caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt attgttttca gtttttaaca
     5101 actgaatagt tgtagttttt acagttctat ttttaacaaa tagaacttga tcaattaatt
     5161 ctttcaaata gtaaaaacga tgttcagcta taacaatagt aaatccttta ttttttaaat
     5221 cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa gacggttcat
     5281 ccaatataat taatttagag tttagcgata aactacatcc aatagaaatt ttctgccgtt
     5341 caccacttga tagcgaaaaa atgtttttat ctaatatatt tgaaagatct aataattcag
     5401 ctaattcatt gattctttta tccatatgct cttttgtaaa tccaaagttt tccatcgaaa
     5461 atactaactc actcgttgta tttgttgtaa agaattgtcc tctaggatct tgacttacgt
     5521 agccaatatt ttttgatata ctttcttgtg ttaaggattg tagcgagtta ttttcaaata
     5581 aaatttcccc aaccaatttt ccgtcataaa gtcttggaat aagtccattc attaacttta
     5641 atagtgtgga tttcccactc ccagaagaac cagtaatcaa tgtaagacta ccttttttta
     5701 ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa aaagaaacat
     5761 tgttaaattc aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg
     5821 gaaaacagga tgatatagtc taataagctc atgtttgttt tatagtaaga acttcttttc
     5881 acaggagatt ctatcccctt tgttattgca ttagcggtca tttcttcagc aatcatagtt
     5941 gcacgtataa gaacgggaac taacagaaat tccaaatatt gcagaggctg aaaaaaagac
     6001 aagcgaatgt ttctcatttt taaagattct ttaatcattc ttatttcaga acgataagtc
     6061 ggaaagaatc tgatcactac tgtaatactt aacattattt ttttcggtaa atgtattttt
     6121 tctaaagagg ataataattc attgctagta tagacactaa ccaacaacca tgagatcatt
     6181 acgaccggca acattcgaaa tacaatatat aaggatgtcc caaaaaatat agtaaaaaac
     6241 tctatcttga tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg
     6301 aaataaatac atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa
     6361 caccccactt gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt
     6421 aaagtaatca aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc
     6481 gcactgttat cttaagctga taaaaatgca ccgttattca gataacagtg cgatcttatc
     6541 aaaaagcata actgagaaca attctcaatg caagcttttt tttaattaac tcagtaattc
     6601 aatccaccca ggaacagaaa attgatacaa tcttctggtt aatagcatag tcttttcttt
     6661 tgaatagcta tgcttcaata tatcatagaa tgtagcgtag aatgagtaaa tgtagaagtg
     6721 gatttcttct tcattaaaga ctaaattttg attgatcatt ccgatagtct ttaacttatc
     6781 tagacaacag actgtctttt ttgtcacaaa tgatgtaatt ttttcaacaa tatcttgata
     6841 tttggttcca gctgattgga ataaaaccaa atcgaatata cgtttattct caaaaatata
     6901 attaataaat ggatctaatt cattgaagtt ctctctccaa cttttctcta tatctatatt
     6961 ttctaagttt aagaggtatt tggtaaactc ttcattatac atattaataa gcgcattaat
     7021 atgcggctga ataatttctt caaataacgc ttccttagaa gtaaaatgtt tataaaaagc
     7081 acccgtagtt acgcctgcgg tcaagcacag ttgccttatc ttagtttgct gaaatcctac
     7141 tttttcaaaa tggcttagtc cactttctat taatttttgg tgagttgtaa tataatctct
     7201 ggtttctatg ggtacttcct cctaataaga tgaattaact atattatctt acacaggact
     7261 tgaagtttca atcgttaacg tctattactg aactcattcc ttagagctaa actaatgaga
     7321 aagagcaaaa taactgatac aaaaagcaat cctacaagaa ttaatagact catctaaatc
     7381 attcaccttt tctaaaataa tccgagaaag atttaaaaaa attatatcca gctattagga
     7441 taagtcctat cataatcact ccaatagcca ttttcttatc ctccctaaaa ataaagcctt
     7501 ttacttaaaa cctttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa
     7561 atcctatatc taaatagtcc tcatatctaa gtttagtcag catctcactt acagatgctg
     7621 gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata
     7681 attcctgaat tgctagtaaa taaattagta tagtagttgt tttaatttgt accataccta
     7741 ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc
     7801 atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata
     7861 gataggaata ttggtatctt ttgatactgt tttcattggt ctatcgtcca cactgctttc
     7921 tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc
     7981 tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc
     8041 tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat
     8101 tgaagcaaat ttagatttag cttctttgtc tag
//
LOCUS       DASJST010000121         8037 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJST010000121 DASJST010000000
VERSION     DASJST010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.123"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="U4B05_002265"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="U4B05_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2051381.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1227..2330
                     /locus_tag="U4B05_002266"
     CDS             1227..2330
                     /locus_tag="U4B05_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2051382.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2523..3590)
                     /gene="rlmN"
                     /locus_tag="U4B05_002267"
     CDS             complement(2523..3590)
                     /gene="rlmN"
                     /locus_tag="U4B05_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN2051383.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3820..6267)
                     /locus_tag="U4B05_002268"
     CDS             complement(3820..6267)
                     /locus_tag="U4B05_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN2051384.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6456..7892)
                     /locus_tag="U4B05_002269"
     CDS             complement(6456..7892)
                     /locus_tag="U4B05_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2051385.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2233 a         1740 c         1375 g         2689 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata tagattatct
      901 tttgctttaa aaaaaaccaa ttatgaattg acaaataaaa cctgttttca tataataatc
      961 ttaattttta gaatttttag ataattacaa aaaagcaaag ggaatcggta gaaatactaa
     1021 gaggaacaaa aattcattat tgtcgttttc aatgtttttg tacttgttcg tttttaggat
     1081 acttattgat gatttcaagg tgttcatgcc accttattca tcacttatcc tgtcttaggt
     1141 tcacctctac tccatctaca gccgcagtca gatcgatcac tctttaaaat tttaacccgt
     1201 ttcaacgaag aaagaaggaa atgaatatga gtcaaagtgt tttcttacca aattatacca
     1261 ttggaacaga tgcgtatgaa aaagcaaatg gaatcgtcaa agaatatggg acaaaagtag
     1321 tgctgattgg agggaagact gcactttcaa aggcagaaaa caaaataaaa gtagctttga
     1381 cacaggaaat agaggttgtc gccagtctct ggtacggggg taattcaact tatggaaata
     1441 ttgaacagtt gatccagcat gaaagtgtcc aaaaagcaga cgtcctgttt gcagtcggtg
     1501 gtggccgtgc ttgcgatact gtaaaagtca ttggtgaaaa actagctaaa ccagtcttca
     1561 cctttcccac tattggttcg aactgttcgc ccgtcactgc ggtatgtgtc atctataacg
     1621 aagaaggcgt aatggacgga ctcttctttc cttcaaaacc acctgtacac accttcattg
     1681 atacagcaat tattgccgaa gcacctgata aatacttatg ggcaggtatt ggcgatgctt
     1741 tatccaaaga agtcgaatct actttttctg ctcgtggcga tgatctaggc tacacggata
     1801 gattaggaat ccaaatcgga aaaatgtgca ttgatcgtct cttgacttat ggtacgcaag
     1861 cattgaaaga tagtgccgac gatcgtccct catatgcaat tgaacaagtc gtttcggata
     1921 tcattgttac taccggattg gtttctgttt tagttaacaa taactataat tctgctatgg
     1981 cacactcatt ctattacggt acgactgttc tagaaaaaat ggagggatac cttcacggag
     2041 catctgtcag ttatggtgta ctgaccttat tgatgatgga tcagcagttc gatttgtttg
     2101 ataaaatcta tgtgtttatg aaaaagaacc aacttccagt atcgttaacc gacatgaatc
     2161 tcaatgtcac gaccgattta gaagcaatct tagataaagc catggcaaca aatgatatta
     2221 aacatgtgcc ttatgaaatc acccgagaaa aatttaaaca ggccattctt gacgtagaaa
     2281 cttatcatgc tacacaagaa agccagcaag gatataagaa aatcagttaa ataattgcta
     2341 aaacccccat caagcaggct gtcatcaaat tgaggacatt tctttgcttg atgagggttt
     2401 ttcttgtcaa aaaaagcttt atagctttcg ctatagaatc ttttctctta tttaagttga
     2461 acagttcctt aattaagaaa actgctcctt ttatggtaat tgaaaaaacg agtaggatac
     2521 gattatttgg caactgtttt tttcatttgc ttgcttcgca attgtccgca agctgcatca
     2581 atatctgtac catgttcttt acgtatcaca cagtttacgc cgtttttctt caatacatca
     2641 taaaatctca atacatcagc ttttggactg cgagaatatt gatcatgttc actgactgga
     2701 ttatatggaa tcaagttgac atatgctaat tttttcttat cttttaatag atctgctaat
     2761 tgttgcgcat gctctggtct atcatttact tgattcaaca tgatatattc aaacgtgatt
     2821 cgacggtttg ttttctctaa atactcatcc acagcttcca tcaatttctc aattgggaaa
     2881 cttcggttga tccgcatgat cgatgttcgc acctcattat tcggcgcgtg caaggaaata
     2941 gccagattga cttgcaagcc gttattagcg aattccttga ttttgtgtgc taatccacta
     3001 gtggaaactg taatgtgccg agcaccaatt gccaatcctt tggcatcatt gatgatatgc
     3061 aaaaagtcca ttacattgtc gtaattatca aatggctcac cgatccccat tacaacgaca
     3121 tgtgaaacgc gttcgccaag attgcgctca tcaaaataat gttggaccat catgatctgt
     3181 gcaacaattt ctccagcagt cagatcacgg ttctttttga gcagcccgct tgcacaaaac
     3241 gtacagccga tattacatcc tacttgtgtc gtcacacaaa ctgacaagcc atattcttgt
     3301 ctcatcagta cagtctcaat catgttttta tcagggagtt caaacaaata ttttactgta
     3361 ccatccgaag cttcttggac gaccatctgt tttagaggat tgatgataaa atgctccgat
     3421 aatttttcga tcaattgttt cgatagattc gtcatttctg tgaaattact tacacgtttt
     3481 tgatacaacc attcccaaac ttgggcagct cggaattttt tctcgccgtt ttctataaac
     3541 caattgatta attcttcatt cgtcaagcca taaatggact gtttttccaa agtattttcc
     3601 ctctttttct caaagtttcg ctacatacta tacatgattc atttccttaa tgcaatactt
     3661 cacaaataaa tcgaatagaa ctttaatctc acaaaaacta aaaagacaag acatcatacc
     3721 gtattagaaa agatggaaaa aaaccatttt ttcttttggt atcatcatgc cttgtctttt
     3781 tattggttat tcgtttttca acccttcatc acacaagttt tacatgtcat tatcgtccgg
     3841 caacgtttct gatgtttctt cctgctcaat gaatactttc cgaggcttgc ttccttctga
     3901 cgggccaaca actccatgag cttccagttc atctactaaa cgtgctgctc ggttgtaacc
     3961 gatccgaaat cttctttgga gaagggaaat acttgccgtt tgcatttcaa cgacaagtgc
     4021 ttttgcttct tcaaacagct catcttgtgg ctgttctgga gcacctgtcg tttcaacttc
     4081 atctgtcggc atcattttct cttcataatg agcttcttgc tgatctgtaa caaattgaac
     4141 gacccgttct acttcgtggt cagaaatgaa tgctccttgc acacggatcg gtttattctc
     4201 acccattggc aagaatagca tatctcctcg ccctaagagc ttttctgccc cattagaatc
     4261 aatgatcgtc cgagaatctg taccgcttga taccgcaaat gccattctgg aaggtacatt
     4321 tgctttgatg attcctgtga taacatccac acttggacgt tgagtagcta ggatcatatg
     4381 aatccccgct gcccgagcca tttgcgctaa acgaataatc gcatcttcaa cttcattgct
     4441 ggctaccatc atcaaatcag ctaattcatc tacgatcact acgatgaatg gtaaggttgg
     4501 atgtttcgtt ccgttttcca agtttttctg ttgaacaaat tcattgtaac cagaaatatt
     4561 tcttactccg gttgccgcga atttttcata tcgttcttcc atttcttgta ctactttttg
     4621 taaggcttgc gctgcttttc tagggttcgt cacgaccggc gtcaacagat gaggtatgcc
     4681 gttgtacata ttcaattcaa ccattttcgg atcgatcatc atcagtttta cttcatgggg
     4741 cttagcacgc attaatatac ttgtgataat cccattaatt gctactgact tcccactacc
     4801 agtcgaacca gccacgagta agtgaggcat cttcgaaaga tcagccgttt gtacccgtcc
     4861 agaaacatca cgtcctaaag gtacttctag taatttatct gggtggtttg gttgtgcttc
     4921 gatgatttct ctaaaggaaa ccatactgat ctttccattt ggtacttcaa taccaatcag
     4981 tgatttacct ggaatcggtg cttccatccg gacatctttc gccgctaaag caagcgcgat
     5041 atcatctgtt aagttcacaa ttttacttac ttttacccct acagccggct ggacttcgaa
     5101 ttttgtgacg gatggtccta agctggcttt gacgaccttg gcatcgacac cgaaactttg
     5161 gaaagtttgt tctaatacgc cgatattttt ttcaattttt ttatactcat cactttgatc
     5221 cacagttggt atagaatcaa gcaaatcaac tgtcggtaat tcatagtctt gattttcctg
     5281 ttctgcttca atctcaaatt ctaatgaggt accgtcatcc tctggcaact catctgaaaa
     5341 accttctgct gtattggtct gttttggtgt atctggtact ggtttctcta gattcgcagg
     5401 atggatattt tcttgaaaac tgtcgattgg aacaaaactt agctgctccg gctctgtatt
     5461 ttctggttcc tgccattcat cagcagtatt ttccctttgc tggctcaccg cttttttctt
     5521 ctcatattct gcagcttctt tttctgcagc ttcttttgca gctaagcgcc ttgcttcttt
     5581 cgcttctgcg cgctgtttca ttctctcttc ctttgccgct tgctttcgag cttgcttggc
     5641 aggatcacct tccaataatt cctgtactcg ttctccaatt gattggatac catttaacag
     5701 ctgatagcct tccagattac tgaacaggaa tgctcctcct gccagtaata gcactgctgc
     5761 taaataactt ccaggctgtg caatcaagaa gtaagtaaaa tgataaagga ttgctccaat
     5821 catcccacca ccaacgttat tgtctacttg actttgtttt atatcactgg ctaacaaatc
     5881 ccatgtcgtc cccatgatat ttggttcccc agtatggagt ttgccaaata aatgggcatg
     5941 taacaataat aagataccga aataaaagag tatgccgcca aaccagcgtc ggtttttacc
     6001 aattgaaaac tctgtattct taatgacaat ccaaaatcct aatacggcta ataaaagaca
     6061 gaggatttgg taagtatttc ctgcaatgat ccgaaagccg tttgcaatca atattcctaa
     6121 aaaacctaat tttaaaaagc cgaagacagc aaataaaatc aaggcaaacc caacaccaat
     6181 cgtcagcatt tgttcctgct gctgtttttg ctttttcgtt tttttcgtct ttttcctttt
     6241 tttagtaggc cgtttctttt gtgccattta ttatccctca tttcatcaag tttcattata
     6301 caaaaaaaag taagggaact aaagttttgt ttgaaagaac tgacgctttc tttcattttt
     6361 cagcttattt tacagttttt cttaatttct aaatgatttt atcaagaaaa ggcacggcca
     6421 tatccagccg taccttcttg ttttaagtat gtgaactaaa ggttctctcc attttcttca
     6481 atcagttttt ggtaccagta ataactgtct ttcttatatc gttccaaaga tccttcttgt
     6541 gtttcgtctt gatcaacata tacaaatcca tatcgctttt gataaccgtt cagccagcta
     6601 agcaaatctg tataagacca ggtgcaatag cctaggactt ctgctccatc cgaaattgct
     6661 tctttgatgg ctttgacgtg gctttgtaag taattgattc gatagtcgtc atggatttgt
     6721 ttatcttctg tcaacttgtc atactctcct aagccatttt ccgtaatcag taccggcatg
     6781 cggtaacggc ttgtgatacg acgcagtccg atccttaatc cttctgggtc aatctcccag
     6841 tcccaatttg ttcgttcgac aaatggattc tcaacttttt tatacattcc aggaactcca
     6901 gtttcttctg atgaaccttt ttttcctgtc gtattcattt ttccagcacc aactccgttt
     6961 aatggattga atgcattcgt cgccgtttga tagtaattga tcccaagaaa atctggttta
     7021 gcagattcta gcaattctgc atcgccagct tcaatcgttg gtgcccatcc tttttcttct
     7081 aagaacctca tggcagcaat cggatatttt ccccacatat agacatccat ccaataattg
     7141 gccatcaggt cttctgcatt ttcagcagcc agaacatttt caggattact gtcaattgga
     7201 taatttggcg tatacgcaaa gcttggtccg atttttccag gcatttctaa ctcgtgaaat
     7261 ttattgatga cagatgcatt tgctaaatta gcaatatggt taacttcata cattcgtttc
     7321 gggtctgtca cacctggcgg atgtgctgct aacaaataac ctaatgaagt gaagatattt
     7381 tgttcattca aactaatcca gtaatggact ttcccgcgga atgcctcaaa taatgtttct
     7441 gcataatgag tgaaatcttc aatgatctga cgtgattccc aaccattata ttcatcttgc
     7501 aatgcttgtg gaagatccca atgatataaa gtcaacatag gttcaatatc gttttcaatc
     7561 aattcgtcga tcaatctctc ataaaaagcg agcccagctt gattgacttc tcctcgtcca
     7621 ttaggaaaaa tccgtgtcca agctacagag aagcggtaag ctttcagacc ttgttctttc
     7681 attaaagcaa tatcttcttt ataacgatga tagtggtcta ctgccaaatc accgttcgtt
     7741 cctttaaatg tctttccagg tatccgtaca aatcgatccc agactgattc tcctttccca
     7801 tcttcttgcc aagctccttc tacttgatac gctgcagaag cagagcccca cagaaaatct
     7861 tttggaaatg aggtaagttc tttgtgttcc attcatctct tcctttccta taaccttcaa
     7921 tcgttcctct tttagaataa cacgaaaccc cttccacaac aaaataaaat tatgtaaagg
     7981 aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggct
//
LOCUS       DASJST010000122         7978 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJST010000122 DASJST010000000
VERSION     DASJST010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.124"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..171
                     /locus_tag="U4B05_002270"
     CDS             <1..171
                     /locus_tag="U4B05_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2051386.1"
                     /translation="INYGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQV
                     PTSKKEDLNTML"
     gene            185..958
                     /locus_tag="U4B05_002271"
     CDS             185..958
                     /locus_tag="U4B05_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2051387.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            972..1784
                     /locus_tag="U4B05_002272"
     CDS             972..1784
                     /locus_tag="U4B05_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2051388.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            1838..2803
                     /locus_tag="U4B05_002273"
     CDS             1838..2803
                     /locus_tag="U4B05_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2051389.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            2919..5642
                     /locus_tag="U4B05_002274"
     CDS             2919..5642
                     /locus_tag="U4B05_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2051390.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            5655..6287
                     /locus_tag="U4B05_002275"
     CDS             5655..6287
                     /locus_tag="U4B05_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2051391.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            7186..>7978
                     /locus_tag="U4B05_002276"
     CDS             7186..>7978
                     /locus_tag="U4B05_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051392.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         2683 a         1215 c         1584 g         2496 t
ORIGIN      
        1 atcaactatg gagggataat caaaaaagaa ggttccaagc aatttagtaa tgccattttc
       61 ttgaatgaat ctgagcaaga agatgccaaa aaattaaaag ctcttggcat taaacaattt
      121 attcaacagg taccaacttc taaaaaagaa gatttgaata caatgttata aaggaggaat
      181 gaaaatgctc gttcagtcaa ttggtttagg gttaatcggt gttttttgta ttttagactc
      241 aaggctttta ggaagaatga attttgaacg accgttgatt gtcagtacgc ttgttggttt
      301 ggtacttgga gacatggaaa aaggattaat tgttggagcc tctttggaat tgatgtcctt
      361 aggattagta aatatcggag ctgctgcacc acctgatatg aatatggcat caattattgc
      421 tacagccttt gccattttat caaatgcgaa tgcagaagca gcattaacga ttgccattcc
      481 aatttcagtg ttaggtcaaa tactaggtat cttaatgcgg acgattttgt caaatttgac
      541 tcacgttgcg gatcgcttta tcgaacaagg gaaatttagc caagcaagga gaattcatat
      601 actttatggc tcgggattat atgctctcat gtattttatt cctatttttc tagcaattta
      661 ttttggaacg aatcttgttt ctcagattgt tgatgcgatt cctgagtggc taacaaatgg
      721 cttaacggtt gcaagtaaaa ttttaccagc atatgggttt gcgttgttat tacaaacgat
      781 gttaacgaaa aaaatggtgc catttttatt acttggattt ttaattactg cttattctgg
      841 attgggaatc attggtattg cagccttcgc atgtattgtt gcgtttgtca tggttcaagt
      901 tcaaggtaaa aactcaaatg gtaaccaaaa tgagccagat ccattagatg acttataagg
      961 aggtagatta agtgaaagag aaagatcaaa atgcatggaa atatcggcag ttcttatggc
     1021 gttcatgggc aattcaagcc tcatggaact atgaacgaca aatgaacatg ggttttatgt
     1081 acggaattgc accaattcta gataacattt acaagaatcc agaagatgaa gcaatgaaga
     1141 aagaagctta cgagcggcat atgatgtact acaactgtac ccctcaaaca agtgcatttg
     1201 tgttagggtt aactgcttct atggaggagc aatactacga agaccaagaa aactttaatc
     1261 cggaatcaat cagtgcagtg aagacttctc tgatgggacc gctatctggt gttggtgatt
     1321 ctttctttca aggaacgatt cgggtattag cctttggact aggaattaat ttagcccaac
     1381 aaggtagtat tttaggtcca attctagcaa tgttaatttc ctttgtacct tcctttttag
     1441 ttacttacta tggtgggaaa attggctata acatggggaa taaatacttg tctaaactgt
     1501 atcaagaagg tttgatggat aaagtaatgt atgtttgctc cattgtgggg ttgatggtca
     1561 ttggttcgat gattgccagc atgatcggga ttacaacacc aattcaaatg ggtaaagctt
     1621 ttgtcttaca agatgtatta gatagtatta tgccacaatt tttaccatta ggattaacct
     1681 tcttcatgta ctggttattg cagaaaaagg tcaaaacagg atggatgttg gcaatctgtt
     1741 tagtcggagg aattattttt agtttgttag gtatcttaca ataagggaat aaccaataaa
     1801 tttggttgaa taatataaat aaggaggaat tcaattgatg aattcaaaag aaattgttaa
     1861 agaaatcgta gcgaaaaaag aggtaaaatc aatcgtattt gttggctgtg gcgcttcaaa
     1921 agcggacctt tatcctgcaa agtattttct agaccaaaat gcgaagcagt tacggattag
     1981 tcattacaca gccagcgaat ttaactatgc aacaccagca tctgtagatg agtcaactgt
     2041 cgttatttca gcttctttag gaggtacgac tccagaaaca gttgaagcta acgcagtagc
     2101 taaagaaaaa ggtgctacag ttattagtct aacacgaagc attgactcac cgttaacaaa
     2161 agatgcagat tatgtaattt atcatcgatt tgcagagaac tatgctgcta aattagaaaa
     2221 aacaggctac tgtttagaat tggcagtaga acttttacat caagtagaag gttatgacca
     2281 ttatgaagca atgcaagaag gatttgctac tatctatgat cttgcccaaa aaagtgcaga
     2341 atcagctcgt aaagatgcga agaaatttgc tgaacgcttt aaagatgaaa aagttattta
     2401 tgtaatgagt agtggtgcta cacatgaagt agcatactca acatcaattt gcttgatgat
     2461 ggaaatgcaa tggattaatt ctggtacctt ccattcagga gaatttttcc acggaccatt
     2521 cgaaattgtg gataaagacg taccgtttat attattaatg aatgatggaa aaactagaaa
     2581 tatagatgct cgagcattaa ctttccttga acgctttgat acgaaaacag aagttgtaga
     2641 tgccttggat tatggcttgt cttcacatat tgctaaagaa gtcttagact actttaaccc
     2701 attcgtgatt actgcagtat ttagagttta tgcagaagaa ttgtcagaag caagagaaca
     2761 tccattaact aagagacgtt atatgtggaa actaacttat taaaaaatag atcaaactaa
     2821 ataaggtggc ttgagaaatt aagccacctt gttttcttca atgtgatttt atacatatgg
     2881 taaaataact agtacatatt gtgaaagcag gtggcaagat gcagcgatta aatgactatc
     2941 tttatcaact aacattgctt ttgaaatcta cgccttcaga taattattca gctcgcagta
     3001 tttcgcaagc tttacagttg aatagaagta caatcagtag ctacttaaat gaaggtgttc
     3061 gtgaagggct tttcataaaa gtaaaaagtt acccggtttt atttttacat agaacagctc
     3121 ttgaagaact acatatcaca ctgaataact ccgaaataga atcaattgaa agcttgttaa
     3181 caatttctca aaaacccgca ttagatcaag taattggctc taaaggaagt ttgaaagaag
     3241 caattgatca aattaaaacg gcagtcttat atcctggaaa agggttgcct ttattattga
     3301 tcggcgctag tggatcaggg aaaacctttc tagcgaataa aatatatgaa tatgcagtag
     3361 aagaaaaggt cattcacaca tcggcacctt tcatagatta taattgtgca caatatgtga
     3421 gcaatccaga gttactatcc tctgctttat ttggctacac gaaaggagca tttaccggag
     3481 caagccagga gcatactggt ttgctagaaa aagccgatgg aggagtattg tttttagatg
     3541 aagtgcatcg cttatctgaa gaagggcaag agaaattatt tacatttatg gatacgggtg
     3601 aattctctcc tatgggggat aatagtattc ggaaaaaagc cgatgttcga ttagtctttg
     3661 caacaacaga aaatatatat acgactttct taccaacttt tcttaggaga ttgccagtta
     3721 ttgttaatct acctagattt caacaacgtc cgtcgtttga acgtctgtca ttgattgatg
     3781 aattttttgt ttctgagagt aaaattttag ctaaggaact atctgtttct gatgcattga
     3841 ttcatttctt aatgaatagt gattttgaag gtaacgtggg gaatattaaa aatattatca
     3901 agtatgcttg cggaaacgca tatatccacc agaagagtca gcaaacaatt caagttaaat
     3961 tattggatat gcctttagag tacagttata aattcaagga gcaactaaat aaacccagaa
     4021 agaaaagagc agatcgaatc tactatccag aagaaacacg acaagtgcaa ctagaaagaa
     4081 acaatcagct ttttgttcaa ttttttgacc aattacttac tggattctct gaagtgattg
     4141 aaaataacaa ggatattaaa ctatttttag aagaaatggt taccaaggta acacaagtaa
     4201 tggatacgct tatttttcaa gaggattacg aaaaagagca gtctttatac gccattctaa
     4261 actatcatat tcgtcaaagt attgatttta tgcgagaaag ctacgggttt gaacaagatg
     4321 gtaaccgggt agtcgctttg gccaactatc tatatgtcaa ggataatcaa gtgatttttg
     4381 aaaatagtct tcaagaaaag aaaaccaaag atcagcttaa cgaatttttt gctaatgttc
     4441 ttgaaacccc ttattggtat gcaaaaaaaa tcttaagcct tttatctgag tgtttagatc
     4501 aacctgtgta tgaagaggac attttcttta ttacgctata tttttatagt ttgaatgtca
     4561 cgaatgttaa aacaggtatt aagagtattg tgctagctca tggttattct acagctagta
     4621 gtatggcgaa tgttgccaac cgtatgctga ataagaacgt ttttcaagct tatgatatgc
     4681 ctttagatat tacattagag aaaattgaag caaaattgat tcaattcctt ggagattatc
     4741 gaacagattc aggcttgatt ttattggtag atatgggttc actaaaccaa ctaggcggtt
     4801 tattagtgaa tcatctaaat ggaccactgt taatcattga tcatatcaat acaccgttat
     4861 tattggaagt cggccaacaa gtattaaaca ataaaagtat caccgaaata ggagcagaaa
     4921 ttgaaacaag tcttactttc aaaaagcagt tactacttcc caagaaaaag aaaaagaaag
     4981 ccatcattac ttgttgttat acagggattg gtagtgcgac ccagattcag gagattttaa
     5041 cgaaatgctt gaaagatgca gttagagaat tagtaattat tccgtatgat tacaaaaagc
     5101 tgttagaaaa caagaatttt gagactcctt ttcaattata cgatgtcatc atgattgtag
     5161 gtacagaaga tcccaaaatt aatcagattc catatattgg attagatcgc ctgattaatg
     5221 gggaagcaat tggcgaattt gttcattact tggaaaaaga gctgactatt gaagtgaacg
     5281 atttacaaaa agaattaatt ttcaattttt caattagtaa aattgtcgaa aatttaacca
     5341 ttttggatcc agataaggtg ctatcttttg tacaaaaagc aattaaagaa ttatcgaagt
     5401 tattaaattt tgatttttct aataatcagc tgtttttgtt gtaccttcac tgtagttgta
     5461 tgattgaacg aattgtacgt aaagaaagtg tggatgagca agaggacatt gaagaatata
     5521 tgcaagcaaa tggagaaata atggataaaa ttcatagcgc tttttttgag attgaaaggg
     5581 aatacacaat tgaaatacca gatttagagt tgcgattatt gagcgatatt gttcaaggat
     5641 aggagtgaat tcgaatgaca atgggagtaa tttttgatat ggatggggta ctcatagaca
     5701 gcgagacatt ttattttgag agaagaatga acttctttca agaactggag atagatccag
     5761 gatcaaacca gattgatgat tatgtgggga aaacagaagc tgcaatatgg gaaacattag
     5821 ttcctgagga caaatcatta agagaggaac tttatccgaa atatgtagat tatcgaaaaa
     5881 cgcatccaat tgatttcgct aaagatttac gaaaagaagt gaaaccgtta cttgacttcc
     5941 tcaagaagcg ggatatacca ttggcactgg catcttcttc tccagttgtt gagatagaga
     6001 gtatgctcat tcaatgtgaa cttactgaat atttttcctt tgttataagt ggtgagtcat
     6061 taaaacaaag caaaccacat cccgaaatat atttaaaagc gaaacagcaa ttagactgtg
     6121 acttctttat tgcagtagag gattcaacga ttggtattaa atcagcaaag gcagcaaata
     6181 tctatacggt ggcactaaaa caaccaatgc aaatcgatca gagtgaagca gacatacaaa
     6241 ttgaaagtct acaagaactg gaacaaattg ttttagaaaa aggttagtga attaatggaa
     6301 ataaggctat atatctgtgc tagctatttt gactaaccaa aaaaacttgc tataattcag
     6361 gtgtagtaaa aggtgctggt aacacctttt acgaaaatgc ttcggcagtg ataatgggat
     6421 tggcttttta agttttttat tttgttcgca atccctaagg tcatgtgaat gatcctaggg
     6481 attgctagaa tattttgcaa cattctatct atttcagaac gtaaccttcc ccagttcatt
     6541 gggattcatt agcatacaac caacatttca aaaagaaatt gcactacacc attattagct
     6601 attttgacta accccaaaaa acttgctata attcaggtgt aggtaaaagg tgctggtatc
     6661 accttttacg aaactgcttc ggcagtggta atgagtgtgg cttaaaggac tagtccttta
     6721 agctttttac tttgttcgca atctctaaga tcaagttaat gatcgataag attgctagaa
     6781 tattttgcaa cattctattt cagaacgtaa cctttcccat tccactgtga ttcattggca
     6841 accctcctca caaccaacgt ttcaaaagaa attgtaaagt tgttggggta ccacgaccga
     6901 acaatactac acctgaatga tagtaagttg tctcattaag tatagcatat cctctttttg
     6961 ggtaaataga aatcggttac attttttatc caagaccaag ttttttcatt tggtcttatt
     7021 tgctataccc aaacaattcc gcagtatagt cgacggtatt gattttatca ggtgagtaat
     7081 agaaacttgt tttccttccc caaaacatta cctataaagg agaaaaaatg gtatcgtaat
     7141 tatataggta taggatgaaa tttttttcta agaggagggt tgttgatgaa aaatttacag
     7201 ttaaaattta ttgttgatcc atcgattgta cgctggtttc ggattatcaa tcaatttgag
     7261 cgcaatcaac ttgccacaaa aggagaatta gcaaagagta atcatgtatc tgaacgaacc
     7321 attcaaacag atgtcaataa aatgaaggac tacttttctg aaagtgttca atttgtttca
     7381 acgaaaagtg ggtattcttt tcagaaggaa agaccggttc tttttctcaa gcagaaggag
     7441 caactactgg agaacgaaat tttatttgag ttgttaggga atgtctttta tggcgaattg
     7501 gaagatatga cggagctaat tgatcgtttt catttatctg aagcgacttt tcgtcggtat
     7561 ttgaagcaga ttcaaccagt tttggaagaa tacggcttag aattgagtct ttatcctctt
     7621 gatttaaaag gagaagaggc aaatattcgc aagtttttta aagacttcta ttatgaaggg
     7681 gagatgacgc cccatactct tgttccacca agggatcttc atgaacttgt gcaagctaca
     7741 ttctatgata aatttgagta tttctcgatt ggaacaggga cttctcctgc tgaattttat
     7801 tatagtttgt atattgcgat tgagcgtgtg agacaaggaa aaacgatagc cattcctcaa
     7861 ggtctgattg agcgagtaat tgctagtgag aattttcgct taatgtattc cttgaaagaa
     7921 gcgattcaaa caaattatca agttgcttta tcagaagagg agttttgttg gttgtatt
//
LOCUS       DASJST010000123         7967 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJST010000123 DASJST010000000
VERSION     DASJST010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.125"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            203..1741
                     /locus_tag="U4B05_002277"
     CDS             203..1741
                     /locus_tag="U4B05_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN2051393.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1738..2703
                     /locus_tag="U4B05_002278"
     CDS             1738..2703
                     /locus_tag="U4B05_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051394.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2690..4012
                     /locus_tag="U4B05_002279"
     CDS             2690..4012
                     /locus_tag="U4B05_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2051395.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4075..4179
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4253..4996
                     /locus_tag="U4B05_002280"
     CDS             4253..4996
                     /locus_tag="U4B05_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2051396.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5011..6003
                     /locus_tag="U4B05_002281"
     CDS             5011..6003
                     /locus_tag="U4B05_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2051397.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6015..6752
                     /locus_tag="U4B05_002282"
     CDS             6015..6752
                     /locus_tag="U4B05_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051398.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6742..7464
                     /locus_tag="U4B05_002283"
     CDS             6742..7464
                     /locus_tag="U4B05_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2051399.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7548..>7967)
                     /locus_tag="U4B05_002284"
     CDS             complement(7548..>7967)
                     /locus_tag="U4B05_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN2051400.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2547 a         1384 c         1740 g         2296 t
ORIGIN      
        1 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
       61 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
      121 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
      181 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
      241 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
      301 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
      361 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
      421 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
      481 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
      541 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
      601 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
      661 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
      721 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
      781 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
      841 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
      901 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
      961 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
     1021 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
     1081 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
     1141 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
     1201 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
     1261 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
     1321 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
     1381 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
     1441 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
     1501 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
     1561 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
     1621 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
     1681 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
     1741 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
     1801 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
     1861 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
     1921 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
     1981 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
     2041 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
     2101 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
     2161 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
     2221 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
     2281 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
     2341 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
     2401 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
     2461 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
     2521 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
     2581 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
     2641 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
     2701 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
     2761 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
     2821 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
     2881 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
     2941 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
     3001 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
     3061 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
     3121 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
     3181 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
     3241 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
     3301 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
     3361 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
     3421 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
     3481 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
     3541 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
     3601 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
     3661 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
     3721 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
     3781 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
     3841 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
     3901 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
     3961 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
     4021 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
     4081 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
     4141 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
     4201 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
     4261 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
     4321 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
     4381 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
     4441 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
     4501 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
     4561 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
     4621 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
     4681 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
     4741 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
     4801 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
     4861 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
     4921 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
     4981 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
     5041 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
     5101 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
     5161 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
     5221 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
     5281 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
     5341 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
     5401 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
     5461 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
     5521 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
     5581 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
     5641 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
     5701 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
     5761 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
     5821 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
     5881 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
     5941 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
     6001 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
     6061 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
     6121 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
     6181 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
     6241 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
     6301 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
     6361 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
     6421 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
     6481 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
     6541 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
     6601 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
     6661 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
     6721 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
     6781 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
     6841 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
     6901 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
     6961 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
     7021 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
     7081 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
     7141 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
     7201 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
     7261 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
     7321 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
     7381 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
     7441 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
     7501 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
     7561 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
     7621 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
     7681 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
     7741 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
     7801 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
     7861 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
     7921 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DASJST010000124         7960 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJST010000124 DASJST010000000
VERSION     DASJST010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.126"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            119..1210
                     /gene="phnW"
                     /locus_tag="U4B05_002285"
     CDS             119..1210
                     /gene="phnW"
                     /locus_tag="U4B05_002285"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN2051401.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1203..1331
                     /locus_tag="U4B05_002286"
     CDS             1203..1331
                     /locus_tag="U4B05_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN2051402.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1335..1880
                     /locus_tag="U4B05_002287"
     CDS             1335..1880
                     /locus_tag="U4B05_002287"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN2051403.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2334..4025)
                     /locus_tag="U4B05_002288"
     CDS             complement(2334..4025)
                     /locus_tag="U4B05_002288"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN2051404.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4445..5392)
                     /gene="arcC"
                     /locus_tag="U4B05_002289"
     CDS             complement(4445..5392)
                     /gene="arcC"
                     /locus_tag="U4B05_002289"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN2051405.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5507..6526)
                     /gene="argF"
                     /locus_tag="U4B05_002290"
     CDS             complement(5507..6526)
                     /gene="argF"
                     /locus_tag="U4B05_002290"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN2051406.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6617..7846)
                     /gene="arcA"
                     /locus_tag="U4B05_002291"
     CDS             complement(6617..7846)
                     /gene="arcA"
                     /locus_tag="U4B05_002291"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN2051407.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2265 a         1623 c         1466 g         2606 t
ORIGIN      
        1 tttattcaaa tttttcaaca ccttgattga atcacaataa tcatcattta tattggatgt
       61 taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat
      121 gtataaatta ttaacttctg gaccattgac gacttctgaa acagtgaaaa atgctatgct
      181 gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga taagaaaaaa
      241 attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa tgcaaggtag
      301 cagctccttc ggggtagaat cggtattaag ttctgtgatc aggccagaag atactgtact
      361 gatttgttca aacggcgcat atagacaacg aatgatcaac atgtgcgaat gtctgaaact
      421 gaactatacc gtttatgagg tatcggaaca tcaaatcctc caagctcatg tgattacaca
      481 gctgattgaa gagaatccgt ctatctccgc actagcaatg gtccatagcg agactacttc
      541 cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaagag atcttttgtt
      601 tatagtggac gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg aactagggat
      661 tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct tttcttttgt
      721 acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt tgtcacttga
      781 tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc
      841 tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat
      901 cgaaactcga tattcacgtt atctcgccaa taatcatgtg ctacgacaaa aaatgacaga
      961 gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct cttttcttta
     1021 ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc aaaatggatt
     1081 tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa atattggcga
     1141 gatctatccg gaagatattt tggaactgac agcaattatc aaagactata aggagaacgt
     1201 taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt ggctgtatgg
     1261 ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aatccaactg actgacaaag
     1321 aaatccgcta accgatggga aaattaaaat gggatcatat ccaacagctt ctttccctcc
     1381 cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa aaagaggtgg
     1441 aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag cattctacct
     1501 tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc aatattggtt
     1561 ccacgaatgg atttaatgct cctatgatga caatcgtagc agaaaccgca aaaaaagaaa
     1621 ggtattcacc agacttcatg gcaacttttg aagacgtcga agggtatggt cgaccgtttc
     1681 cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat gaagtaatca
     1741 aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg ttgacagtag
     1801 gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa aaattagaga
     1861 tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag ccggcgctga
     1921 tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg acatgaatcc
     1981 agaatgactt ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc aagtaataaa
     2041 aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttaggcttat taccgcagat
     2101 gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac gacttttgtc
     2161 acagtcccct tatttgcaaa caaacagtgt ttaagagaca acctctccac taaacggtgt
     2221 tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa gctattttct
     2281 cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt ttcttacatc
     2341 ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac agtcactgca
     2401 taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt cgtatgagca
     2461 tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac tgacggttcg
     2521 tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt aaccacttcc
     2581 cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa tcctgctttt
     2641 tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt ttctccttca
     2701 aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg
     2761 tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt ttccaaatca
     2821 gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa tacttcttct
     2881 aataagacga tctttccttt acgagttgat aattttttac caccttgcgt gatcaaacca
     2941 aatggaatat ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa gacagctttc
     3001 aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg tttgaagtcg
     3061 taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg
     3121 tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac gatctctgca
     3181 cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc atccatcttg
     3241 tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag atcatagatt
     3301 ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat agcctcttta
     3361 tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc tggttctgtt
     3421 tcagctactt catggaattg gacgtataaa cgaagcaatt cattgattgg ttctgcttta
     3481 acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc
     3541 cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat aatgaaaccg
     3601 atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt ggcaatatta
     3661 ggagaagaca tgtcgattgg tactgttcct tgattcccga tgttgctatc cccataatgt
     3721 tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt attcatgaaa
     3781 aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc gattttttct
     3841 gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct
     3901 ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg ctcgacttgt
     3961 tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag atctttatta
     4021 ttcatcttta ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa tctctagcga
     4081 cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac tccaattgtc
     4141 ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa aacagaattc
     4201 tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga attcgctagc
     4261 caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa ctttcttgaa
     4321 ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa aaaaacaatc cagatggcct
     4381 aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa
     4441 caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt
     4501 ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgctt
     4561 ctactttcgg aagcatgctt cctggagcga attgcttttc atcaatgtat tgtttcattt
     4621 ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat
     4681 tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg
     4741 aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa
     4801 caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga
     4861 tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac
     4921 gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt
     4981 cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat
     5041 ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta
     5101 cttcgcccat ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg
     5161 tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt
     5221 taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt
     5281 tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg
     5341 tagacaagat ggcattgccc cctaaagcga caactacttt tcgtttggcc atttcattcg
     5401 tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt
     5461 tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga
     5521 taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg
     5581 cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag
     5641 tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg
     5701 gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat
     5761 tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg
     5821 atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg
     5881 taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt
     5941 cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg
     6001 tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac
     6061 cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat
     6121 gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta
     6181 ccattttttg actgaatcca cggaattcga ttccgtcaaa catacgtcct agtactttag
     6241 ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct agatattcag
     6301 gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt
     6361 tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt
     6421 tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt
     6481 ctttttctgc taataagctt cttccttgga atactgattc tttcatatta aacattctcc
     6541 tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagact
     6601 gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg
     6661 gaccaccacg acctcgtgac aattcacttg aattgatttc taacactttg atgccatagc
     6721 tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga
     6781 ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat
     6841 taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta
     6901 agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca
     6961 tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg
     7021 taaatacggt atccaaatgc atcattgcac ggttgttcgg aattttgatt gccaacactt
     7081 tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat
     7141 tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc
     7201 cttcgatatg atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc
     7261 ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag
     7321 tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca
     7381 agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcatatagac
     7441 tgctagaacg tacttcgata tcttttgtac gaaccccagc catgatttta tctaccattt
     7501 cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa
     7561 tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg
     7621 cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtcttcg
     7681 caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac
     7741 gatccataat atctggtgtc aaattttcca cttcttgtcc aggccgtttc agtaaaactg
     7801 tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc
     7861 ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct
     7921 cattcaaaat gagacattcc tcacattaat attcgataat
//
LOCUS       DASJST010000125         7959 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJST010000125 DASJST010000000
VERSION     DASJST010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.127"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            192..380
                     /locus_tag="U4B05_002292"
     CDS             192..380
                     /locus_tag="U4B05_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN2051408.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            458..1471
                     /locus_tag="U4B05_002293"
     CDS             458..1471
                     /locus_tag="U4B05_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2051409.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1538..2572)
                     /locus_tag="U4B05_002294"
     CDS             complement(1538..2572)
                     /locus_tag="U4B05_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2051410.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2687..4912
                     /locus_tag="U4B05_002295"
     CDS             2687..4912
                     /locus_tag="U4B05_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2051411.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            4930..6813
                     /locus_tag="U4B05_002296"
     CDS             4930..6813
                     /locus_tag="U4B05_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN2051412.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6794..7768
                     /locus_tag="U4B05_002297"
     CDS             6794..7768
                     /locus_tag="U4B05_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN2051413.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2586 a         1452 c         1680 g         2241 t
ORIGIN      
        1 aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga cccattattt
       61 tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg ctgggagttt
      121 tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg actgtaacga
      181 aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa gcgacacgat
      241 atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca acagaagaag
      301 aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca ttgcagaaga
      361 agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac actatggtat
      421 aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa ttcttaaatt
      481 tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc ttgccttcca
      541 gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata ctgttcaaat
      601 cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa cagatttgac
      661 gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa acagcaaggg
      721 gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag ataaatcggg
      781 tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg ttgcgatgaa
      841 ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga atgaactgat
      901 caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac aattattttt
      961 tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac aaacgaatga
     1021 tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc tgaaaggaac
     1081 catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt cggatgatcg
     1141 atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa aagattggaa
     1201 aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg aatttccgcc
     1261 aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag cattagaaaa
     1321 tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata caaaaaaaga
     1381 gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt gttaccagca
     1441 gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt ttcgttttga
     1501 gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt gttcttggga
     1561 taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt tcgtcccgta
     1621 taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat ttcgcattga
     1681 tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta tcaaagcttg
     1741 ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga tagacaccaa
     1801 ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca ttttctaata
     1861 gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga taagacatat
     1921 atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc ttttcattta
     1981 tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg atataggcta
     2041 ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc cgttcaagat
     2101 ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg actatttgca
     2161 tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca acgatcaccg
     2221 cacctaactg atctagatct tcccattgtt taggattatc agatagatta tagattcgat
     2281 tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa tgtttgtaat
     2341 agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc gaaggcattc
     2401 tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct gcctctacta
     2461 tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac acacgggaaa
     2521 ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc atttttacac
     2581 actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca gtatacagga
     2641 attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg atattacgca
     2701 tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct tccatttacg
     2761 aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg cgcatattta
     2821 ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa gagatcgtgg
     2881 attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata cactaccgca
     2941 agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca ttcagcgcaa
     3001 gaatggatca gacttactgg atctgagata tgacagccat tatattacag atgggaagcc
     3061 agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac aaacgttggt
     3121 gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata ccatatttac
     3181 tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag aaacaatcaa
     3241 actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat ttgatgaagt
     3301 cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat ctgttttatc
     3361 tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga ataactttat
     3421 tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac ttcaatttgt
     3481 ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat tgagagtcgt
     3541 cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt cttttcaaac
     3601 acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc agatccacca
     3661 tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac ggccgattct
     3721 ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca aggcgatcat
     3781 tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct ggtttggaaa
     3841 aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca agttgacaaa
     3901 tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg gtttatggtt
     3961 tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg attttcttat
     4021 gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg attttacaag
     4081 gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata cgattcctct
     4141 tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga ttacccttga
     4201 cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg aactacttga
     4261 acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg gaggtggacg
     4321 gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata acacggatgc
     4381 agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt cgtccatcac
     4441 agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt tgaaaacaag
     4501 aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa agctgtctcc
     4561 tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc gtccggtgat
     4621 ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg ccgcatggat
     4681 gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac tagcttcagc
     4741 tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac tttatcagga
     4801 acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac tttacttccc
     4861 cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat gagagagaag
     4921 gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt tagagtgaac
     4981 caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt agaaaccaca
     5041 tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc aaatccttcg
     5101 gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga tgaaatcact
     5161 gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa tacgatgtat
     5221 ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga gaaagaggcg
     5281 gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac gacttttcga
     5341 ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt agaacaagca
     5401 atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt cactccgtct
     5461 gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga ggtttacaaa
     5521 agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg gatcttcaga
     5581 gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat caaacctcgc
     5641 ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga gcaagtagcc
     5701 aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat ctatcaacag
     5761 caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa tgtggcactg
     5821 tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga cgaagaaaaa
     5881 agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat caaagataaa
     5941 atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca tgagtggcat
     6001 ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc tatcataaaa
     6061 aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac ttggtattat
     6121 ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc ccatggctca
     6181 tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt acctcagtgg
     6241 caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa ccatccaagt
     6301 attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg acaaatgaac
     6361 gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa ttttccaaat
     6421 ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc accagaagag
     6481 atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga atatatgcat
     6541 gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga tcgttatgaa
     6601 atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt gactgatcca
     6661 atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc aagtgactat
     6721 gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc catgcaggag
     6781 gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg aaaaagttgc
     6841 aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt atattttttc
     6901 ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc tataccggga
     6961 aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg gatttggatt
     7021 taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa tagaagtact
     7081 ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat attcaagtaa
     7141 tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag taccaaaaac
     7201 ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca atatgcatta
     7261 ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca ttatgccaac
     7321 gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg atcggatggc
     7381 tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat accttgttcc
     7441 ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa atcaaacgtt
     7501 ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc aggatacgcc
     7561 atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa cacatataga
     7621 agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa gaggagtcgg
     7681 gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca gcgaagagga
     7741 ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga aaaacggagt
     7801 ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa aagttctcag
     7861 atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc tgaaaaacaa
     7921 agttaactat cagaaaatta aaaaaagttt cataaaaat
//
LOCUS       DASJST010000126         7834 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJST010000126 DASJST010000000
VERSION     DASJST010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.128"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1415
                     /locus_tag="U4B05_002298"
     CDS             156..1415
                     /locus_tag="U4B05_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2051414.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1421..2002
                     /locus_tag="U4B05_002299"
     CDS             1421..2002
                     /locus_tag="U4B05_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN2051415.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2084..2959)
                     /locus_tag="U4B05_002300"
     CDS             complement(2084..2959)
                     /locus_tag="U4B05_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2051416.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3107..3439
                     /locus_tag="U4B05_002301"
     CDS             3107..3439
                     /locus_tag="U4B05_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2051417.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3551..4759
                     /locus_tag="U4B05_002302"
     CDS             3551..4759
                     /locus_tag="U4B05_002302"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN2051418.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            4913..5368
                     /locus_tag="U4B05_002303"
     CDS             4913..5368
                     /locus_tag="U4B05_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051419.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            5730..6005
                     /locus_tag="U4B05_002304"
     CDS             5730..6005
                     /locus_tag="U4B05_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051420.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6018..>7834
                     /locus_tag="U4B05_002305"
     CDS             6018..>7834
                     /locus_tag="U4B05_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN2051421.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGI"
BASE COUNT         2618 a         1294 c         1663 g         2259 t
ORIGIN      
        1 ggaaaaagtt ctttttttgg cagaaaaaag caaaaaaata cttgttttag aaaaaatagg
       61 tataatagac ggataccaac atccaaacga aaaagaatta ttgagaacaa tgacggtatt
      121 tcaacaaatc gtcagtgaga taagagggaa ttgaaatgaa tacaaatagc gaaaaaatgt
      181 tgcaagcttt atcagatgaa gacttgtcac aagcacaaat ctttttagaa aaagcattaa
      241 aagaagaccc agcagatata ttagctgaac taggcgaaga attgctggct attggctttt
      301 tgatcgaagc aaaacagatt tttgaacaac tgatcaacca atacccagaa aatgacggac
      361 tgaacatccc gttagcagaa attgcaatcg aagataatga gatcgactta gcttttgaat
      421 acctggaaaa agtggataag caaagtgatt actatccgca aagcttattg gcgatagctg
      481 atttgtacca agttatcgga atccctgaag tgagtgaagc aaaattgaaa gaagcagctg
      541 ggttgcttcc tgatgaacca ttgatccagt ttgcgctagc tgaattgtat ttttcagttg
      601 atcgtttcaa agaggcagca gtggtttatg aaacattgtt ggcaagcggg ataaatgaaa
      661 tttcaggaat ctcgatgcag gaacgtttag gacaatcctt aagtatgcaa ggagaattcg
      721 aagcagctat cgtaccttta gaagaagcac tgaaagaaga aaggacagat gatcgactgt
      781 tccagctagc ttttacgaat cttcaattga aagaaaatca gcaggcaatc aactatctgg
      841 aagaacttcg agaagttaat ccgcattatc aatcgttata tctatatcta ggacaagctc
      901 ttcaagaaga agaaatgatt gaagaagcac aaacagtgtt agaagaagga atcaaagaaa
      961 atccttacca agtcgaactt tatcacctgg cctcggaaaa tgctttccgt ctgcatgata
     1021 gacagcgagc agaagaactt ttactgaaag ctttagaagt aggagacaaa caagatgaaa
     1081 cattacttac tttaagtaat ctttacttgg atgatgaaag atatgaagat gtgatacaag
     1141 tgatcaatca gatggaagaa actgctaatc cttatgcaga atggaaccta gcccatgctt
     1201 ataatgaatt agaagacttt accgttgcag ctgtacacta tgaacaggct tatcatgaac
     1261 tatcacatga accagatttc ttgaaagagt atgccttttt cttaagagaa gaagggcagc
     1321 tgaaaagagc gcaagaatta ttgacttatt atttaacact tgaaccagga gatatggagg
     1381 cactgtcatt attagatgac ctaacagaga ggtgagaaaa atgctaattg atgtacggga
     1441 aaagaagaat tttttaacgt ggatggtcaa tcatgtatct tttagccgaa gagaagtttt
     1501 ttggatcttg aattatcttg ccaatcatga agcgatttta agcaatgtcc actttgttga
     1561 aggggcgaat tttgcggatc gaggactgca gatcagtgat ctatctgttg aaggggaacc
     1621 catgaaattg tttttgcagg gaaaagaatt tacggacaca gaccagattt ttcatgaaat
     1681 ccgtttgaat tggaaaaagc ctttatatgt ggaatgtata ttcgaaaatg cttggcagac
     1741 aagagaatat ctatctatct tagaagacaa tccattttct tcttgggatt cttcagtcag
     1801 tgaagaagat actgaattaa ttgaacgata ttttcaagaa aaagaagaag aggcaaaact
     1861 aaaaatgctt tatgctcaaa tcgatcaagc actagaagat ggagataggg atgctttttt
     1921 gaaattatct gatgaagtta accgaaagat ccttttgcag acacaaaaga aaagtaaccg
     1981 ggtaagtgaa cataacaatt agttaaaaat agtatagtac taaaaaagac aaaggtcagc
     2041 tggatgtgga atctttcctc atagcctatc tttgtctttt ttcttatagt tttttcagta
     2101 atcttgtctg tggcttcaaa taagtaaagc cttgcccttc cacttcatgc acattgatga
     2161 ttgaaaggaa agctttttcg tcaatctcgt ggacgatcct cttagcttca gttaattcac
     2221 tagagctaac gaccatatag atgatttttt ttgaattacc agagaatgcg ccttctccag
     2281 aaaggaaagt cacacctctt tgcagaccgg tcatcagtag tggtgcaatc tcttcgcttt
     2341 tgttggagac gacaagtaga ccttttgcag aatatccgcc atctaagatc gaatcaatca
     2401 cacgactgaa aacatacgaa acaatcaacg tatacatcat acgtttcaag tcgatatagg
     2461 ttaatgaagc aagtaagacg atgatatcaa atgccaatag ggaacgcccc atagtgatgc
     2521 cgtaattttg ttctaaaatc cgagcgataa tatcactgcc gcctgttgtt ccacccatgc
     2581 ggtagacgat cccgcttcct acacctgcaa ctaaacccgc aagtagagaa acgatcagta
     2641 gatcatgctg taaattgatt tctagtggaa ccctttgcca gatccataaa aagaatgaca
     2701 aagaaagtgt acctagaaca gtataggcca aggaacgttt accgaggatc tttcctccta
     2761 gtaaaatcaa tgggatgttg attaataaag tcgtataagc tggatcaata ccaaataaag
     2821 ctcttaagat caaggtgatc cctgagactc ccccttctgc taaatgatta gcgatgttca
     2881 agtaaacaag accaaatgca taaatacttg ttcctataat gataaaaaga acttctttta
     2941 ctgtttttcg tttgaccata tgaattccag ctttcaatta tgtattttca cagaatttcc
     3001 atttagaaat tccttcctaa atgtagcatg gaaattcgat tttctcaatt gctcttatct
     3061 aggatttgcg ttaaagtttt ggtaagatag ttttggaggg aaaaagatgg ataaaaagcg
     3121 ttctttatac agtatgcagc aggaagtgga tgaatatatc cagcaattca aggtggggta
     3181 tttctctcca ttagctcaga tggctcgatt aactgaagaa gtaggtgaat tggcaagaga
     3241 agtcaaccac acttatggag aaaaaagcaa aaaggcttct gaaccagtta actcggtagc
     3301 agaagaactt ggtgatgttt tattcgttac gatgatcatg gctaattcat tgaatatcga
     3361 cttaactgaa gtttttgaaa aaaacatgga aaaattcaat caaagagacc ataatcgctt
     3421 tgagcgtaag gacgagtaaa caagatgaag tggtcctggc cttgaagact ctgaaacgga
     3481 cttgtcaaac agtcaccatg caactgagtc tttatctcag gacaagaaac acgcaggaaa
     3541 ggttggaaaa atgaaattaa aacaattacc tctggaatac caaaaggcaa ttccagttct
     3601 gaaaaaatta gagaatgcag gatatgaagc ttattttgta ggtggaagtg ttcgtgatat
     3661 cttgctgaac cagccaatcc atgatgtgga tatcgcaaca agcgcatttc cagaagaaat
     3721 caaacagctt tttccaaaaa cgatagatat tgggatcgaa catggaactg tgttggtatt
     3781 agaaaatgat gaacaatatg agatcaccac atttcggacg gaatcaactt atcaggacta
     3841 ccgtagacca gatcatgtcg aattcgttcg ttcgttggca gaagatttga aaagaagaga
     3901 ttttacgatc aatgcgtttg ctttgaaaga agatggagaa atcatcgatt tgttcgatgg
     3961 tctgcaagat ctagaagaac agatcttgcg agctgtaggc aatcctcatg agcggttcca
     4021 tgaagatgcc ttacgaatga tgcgcgggtt gcgttttgtc agtcaactag ggttcaaatt
     4081 agaggaagaa accttctacg ccatcaaaga aaaccacgag ctattaggta aaatatctgt
     4141 agaacgtata aatgtggaat tcatcaagat gctcttggga aatgaacggg ccgcgggaat
     4201 ccatgcattt attgaaacag agtgctacat ttgctgtccc ggtttgaaag aagaaggcga
     4261 agcactttta cgttttgctg aattaccatc cgtcccattg aaagaagaga gccaagcatg
     4321 ggcattattg atcagccaac tggatctaaa agaaaatgag attcgttcct ttttgaaatc
     4381 gtggaagtgt tctaaccaaa tgattaaaga cgttcaagca cttgtccaag gactacgaca
     4441 acgagaaact ggagctttgg aaccattcga attgtacttc ttaggcaaaa aacatgcact
     4501 gcaagtagaa gaattgatga tctattttga cgaaacacct cagatggatg ccgtaggaga
     4561 atcctatgat cgtttaccta tcaagtcatt aagcgaatta gcagtagatg gtaaaatctt
     4621 gctagaagaa actggaaaaa aacctggtaa gtgggttagt gaagctttgc aattagctga
     4681 aaaagccgtc gtagaacaaa gaataaaaaa tgataaaaaa caggttttgg attttttatc
     4741 gaaagaaaaa ttgatttaaa aaacactaga ctgtatctgc tcagctttgt tggctactta
     4801 aagtgggcag atacgtcatt agaagaaaaa acataagatg tgaccactta cataaatttg
     4861 tgctaaactg aggtagtaca agaataaatt acatcataaa ggagtctttt taatgagtaa
     4921 agaaatgaca gcgctaaaat tttattttag aaatggagaa acctggacga tcaaccgccg
     4981 tcatatcggc gatctttgga tcaaacaaat cacaacaagt tttggacgaa tcaatggcag
     5041 tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc gaaatcttcc atgaagggga
     5101 cgcagttgcg acacatgata tcaacctagg cggactggaa atgggcatgt ttaatcgcgc
     5161 attgaaatac gaagatatcg agcgaatgga aatcctttac cgaaatggca cacctgatct
     5221 tgtgtacttc ccatatctag ataaaggaac agaaggatta gacaatcaat atcaatcaac
     5281 aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc aatccagatc aacgtgtaga
     5341 agatgtatac ggggaatttt tcgaataaat cgatttttac gcaaaaatga ggctgtgaaa
     5401 gagatgtgtc ttattactac tgataggctg tctcttccac accgtttatt cagaataagg
     5461 gactgtgaca agatttttgt cgcagcccca tttttttgtt gtgataaaag aacaagaaag
     5521 gcaggaatgg gaaagtaaac atccttttgt cagaaacttt tatctttctt aagttttact
     5581 tgatatctga tactatttgg aatagaatga ggcgttgaaa aatttatgga aatttctaac
     5641 tagcaataaa cgatgaaaca atgaaatatt tttcatccaa aaagaacttt gctataatga
     5701 tggaagtttg tgtataggag atctggaaaa tgagagagag ttttgaacaa caaaaaaaac
     5761 tgttgcatga tcgctatggc gccctttcga tggatgaccg ccgtcaaata ctttgtaagt
     5821 tgcgtaaaag aaatatattg atgtatcgtc agttggaacg tctgaaacat gatctgcttc
     5881 gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa ccaaacacag gttgaagtag
     5941 tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa aatgcttaca caaaataaaa
     6001 aataaagggg atggacgatg gaatttgctg agttagttat tgtatttgca gtaacgatca
     6061 ccttatcgaa catagctagc cgcattttgc caatgattcc agctccactg atccaaattt
     6121 tccttggagt ggtattaggt ctgacagagt ggggacaatc aatcgatttt gaacctgaat
     6181 tgttcttagt tatgattatt gcaccattgt tatttagaga aggagaaaaa gcagatattt
     6241 cttcgattct caaaaatttc gggacaattt tgttcttggc atttggagga gtcattttga
     6301 cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc aagtgtccca cttgctgcct
     6361 gctttgcttt tggagcagcg ttaggtccga cagatgcggt agcagtcagt tctctttctg
     6421 gacgagtgaa tatccctaaa aaggcgatgc atattttgga aggagaaggt cttttgaatg
     6481 atgcatctgg tgttactgcc ttccagtttg ctcttggtgc gctgatcacc ggatcttttt
     6541 cagcagtaaa cgcggggatg agtctagtag tttctagtat tgggggagct ttgatcggct
     6601 ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat cgaaaaagca tcagcacaag
     6661 atgtcacagg gtatctgctg atcgaacttt tgcttccttt tctagcctat gtattggcag
     6721 aggcatttga tgtatctgga atcattgctg cagtggcagc aggtattctc caagcttctg
     6781 gttttaggaa aatctctgtg tttgatgcag aattgtcgag cttgtcccac agtacatgga
     6841 caactattgc ttttacactg aatgcattag tatttatatt tttaggaatc gaattgacgc
     6901 aagtgttctc gccagtatgg ggagacggtt tatatccaaa cggtctttta ttagcgatta
     6961 tcgtattaat ctctgtcatg ctttttgtga tcagattcat ttctatcagt cttttttatg
     7021 tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa cgagatcttg atcttaacat
     7081 ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt tattttgcca ttttcaatca
     7141 ataatatgat gttttaccag cggtctttgc ttttgtttct gacggcgggt gtcatcttag
     7201 tgactttggt catagggatc attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa
     7261 gcacggattt gaatgctttg atgattttag aagaggtcgt tgaagttttg cgaaaagaaa
     7321 taaaggaaat tgatcgtaat acaaaagaat ttttagccac tgaagcagtg atagaaaatt
     7381 atcaagaacg gatacgtgat ctgtacttgg aagatttaac cgatgatgaa aagcaagaag
     7441 tacaagaaat acaagcactg attttgtcga tcgaacggga tggattggat gaaagttatc
     7501 gttcgggcaa gctttcttct aatggttatc gtttttactc ccgttttctt tctcgattcg
     7561 aacattctat cacaagccag attttgtctt ttatcggctt ttggttcatt gttgtccgta
     7621 ggttaatgcg aatcgttctc catccgaaaa tgttttggca aagaagatat gctgatcgtc
     7681 agacatttat tagtgatgaa gatatccaag aaatcagaga gacatatcaa aaaaatactc
     7741 agttgattat tgaaagttta gataacttga ctgatattta tgatgaaacg atggttcaat
     7801 tttttatcca gcaacgcaaa cttgaaggaa taaa
//
LOCUS       DASJST010000127         7831 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJST010000127 DASJST010000000
VERSION     DASJST010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.129"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..922
                     /locus_tag="U4B05_002306"
     CDS             41..922
                     /locus_tag="U4B05_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051422.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            935..2545
                     /locus_tag="U4B05_002307"
     CDS             935..2545
                     /locus_tag="U4B05_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN2051423.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2622..3281)
                     /locus_tag="U4B05_002308"
     CDS             complement(2622..3281)
                     /locus_tag="U4B05_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2051424.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3564..4229)
                     /locus_tag="U4B05_002309"
     CDS             complement(3564..4229)
                     /locus_tag="U4B05_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN2051425.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="U4B05_002310"
     CDS             complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="U4B05_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN2051426.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="U4B05_002311"
     CDS             complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="U4B05_002311"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN2051427.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="U4B05_002312"
     CDS             complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="U4B05_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN2051428.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6543..6863
                     /locus_tag="U4B05_002313"
     CDS             6543..6863
                     /locus_tag="U4B05_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN2051429.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6975..>7831)
                     /locus_tag="U4B05_002314"
     CDS             complement(6975..>7831)
                     /locus_tag="U4B05_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2051430.1"
                     /translation="KKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSGA
                     ENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALEA
                     AKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKEF
                     NQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRIK
                     DILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAAA
                     YGIIRKKK"
BASE COUNT         2024 a         1636 c         1526 g         2645 t
ORIGIN      
        1 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
       61 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      121 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      181 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      241 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      301 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      361 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      421 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      481 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      541 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      601 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      661 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      721 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      781 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      841 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
      901 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
      961 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1021 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1081 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1141 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1201 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1261 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1321 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1381 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     1441 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1501 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1561 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1621 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1681 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1741 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1801 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1861 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     1921 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     1981 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2041 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2101 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2161 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2221 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2281 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
     2341 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2401 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2461 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2521 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2581 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2641 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2701 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2761 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2821 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     2881 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     2941 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3001 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3061 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3121 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3181 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3241 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3301 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3361 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3421 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3481 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3541 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3601 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3661 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3721 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3781 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3841 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     3901 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     3961 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4021 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4081 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4141 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4201 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4261 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4321 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4381 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4441 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4501 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4561 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4621 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4681 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4741 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4801 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4861 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     4921 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     4981 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5041 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5101 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5161 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5221 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5281 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5341 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5401 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5461 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5521 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5581 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5641 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5701 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5761 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5821 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     5881 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     5941 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6001 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6061 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6121 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6181 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6241 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6301 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6361 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6421 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6481 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6541 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6601 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6661 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6721 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6781 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6841 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     6901 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     6961 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7021 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7081 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7141 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7201 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7261 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7321 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7381 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7441 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7501 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7561 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7621 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7681 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     7741 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7801 ggccagatta acggcttcat tctttttttc a
//
LOCUS       DASJST010000128         7797 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJST010000128 DASJST010000000
VERSION     DASJST010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.130"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="U4B05_002315"
     CDS             complement(105..629)
                     /locus_tag="U4B05_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2051431.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4B05_002316"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4B05_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN2051432.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4B05_002317"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4B05_002317"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN2051433.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U4B05_002318"
     CDS             complement(2351..3031)
                     /locus_tag="U4B05_002318"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2051434.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U4B05_002319"
     CDS             3157..4026
                     /locus_tag="U4B05_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2051435.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U4B05_002320"
     CDS             4052..4567
                     /locus_tag="U4B05_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051436.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U4B05_002321"
     CDS             complement(4614..5207)
                     /locus_tag="U4B05_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2051437.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U4B05_002322"
     CDS             complement(5232..5411)
                     /locus_tag="U4B05_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051438.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U4B05_002323"
     CDS             complement(5488..5997)
                     /locus_tag="U4B05_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051439.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U4B05_002324"
     CDS             complement(6093..6473)
                     /locus_tag="U4B05_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2051440.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="U4B05_002325"
     CDS             6803..7552
                     /locus_tag="U4B05_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN2051441.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2232 a         1614 c         1382 g         2569 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaa
//
LOCUS       DASJST010000129         7765 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJST010000129 DASJST010000000
VERSION     DASJST010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.131"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..143)
                     /locus_tag="U4B05_002326"
     CDS             complement(<1..143)
                     /locus_tag="U4B05_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2051442.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQ"
     gene            complement(154..351)
                     /locus_tag="U4B05_002327"
     CDS             complement(154..351)
                     /locus_tag="U4B05_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2051443.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(374..667)
                     /locus_tag="U4B05_002328"
     CDS             complement(374..667)
                     /locus_tag="U4B05_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051444.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            complement(704..841)
                     /locus_tag="U4B05_002329"
     CDS             complement(704..841)
                     /locus_tag="U4B05_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2051445.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            complement(843..1289)
                     /locus_tag="U4B05_002330"
     CDS             complement(843..1289)
                     /locus_tag="U4B05_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051446.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            complement(1292..3478)
                     /locus_tag="U4B05_002331"
     CDS             complement(1292..3478)
                     /locus_tag="U4B05_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN2051447.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            complement(3478..4260)
                     /locus_tag="U4B05_002332"
     CDS             complement(3478..4260)
                     /locus_tag="U4B05_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051448.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            complement(4277..6034)
                     /locus_tag="U4B05_002333"
     CDS             complement(4277..6034)
                     /locus_tag="U4B05_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN2051449.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(6009..>7765)
                     /locus_tag="U4B05_002334"
     CDS             complement(6009..>7765)
                     /locus_tag="U4B05_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN2051450.1"
                     /translation="DVRKQTASIVNNINSLESSKMTFYESTEIGKYQDDIMRGAGDNG
                     GSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFKQCKKGEWQTIQ
                     DVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQLVAEGGELSFEK
                     QVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDGGDRGNPIFQIP
                     ADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGGNSGGNDNGWNG
                     QYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPTADEGNGAPGFG
                     YGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAPNGQWIATSAYP
                     YTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDLEIPVSKGYMKP
                     IADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEVIVAGDANYFDW
                     YGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPSTGEHLHFQFMDE
                     FYPSSSGHFHNARDYIDF"
BASE COUNT         2009 a         1747 c         1401 g         2608 t
ORIGIN      
        1 tactgattat ttgctacttg tgagctacct tcgtttgggc ctaaattaaa ttcattgtta
       61 atcgtatagg caaacccatt aattggcaat aaaaaaagag ccgttaatag gctcatcgca
      121 gtaatagtaa ttttcttttt catttgtttc ctcctatttt ttcaaattat aagccgacac
      181 gccagtgata acgcctaaaa atgttgctac tgcattgata gtgagtactg tcatatctgt
      241 tccattccat ccatacgctt tgcctagtgt tgcaactaat acagatgcag ctggcaatac
      301 tgttaaaact gtccatttaa tgacttgata atacttatcg ggtaaaatca tttcttctta
      361 actcctttct tttttaccga gatctttctc taaataaagt tttgatttgt tgtgtgtgtt
      421 ctaccaattt ttctgtatgt gtatctaatc tttcatcgtg tttctttagt tcttcatgaa
      481 tcatcaatcg atctgatttg ctcgattcta aatctttagt cagcaaatct aaattgtgac
      541 ttacttttga aagagtttca gtaatcttcg agaaagatgc agtgactggt cttattacta
      601 ataaaatcaa agaaacaatc gcagtgattg atcctgcgat tgttccccat tctcctaaat
      661 taatcatgtg acaactcctt taccttaaat aaaaacgcat cacttaagat gcgctcttat
      721 ctttattaat gattttttct gcttcttcgt ctgtaatgca tagtggaacg aatagtcgaa
      781 cttgatcgtc agtaaaacag ccccaatcat acatcatttt cacatcgcta aaactaaaca
      841 tactactcac ctccctttga agctggattt agttgctctt taatttctgc aatgtctttg
      901 ctattttgga acgaagcaag catcgttttt gaattgattt gtgctaaacc atccgctttt
      961 tctttcaatg cagtattttc ctgtttaatc gctacatcgc ttagcatgag tttggcgttg
     1021 agctgtttta aatcgccgtt ctcattttct aacgactcat acatcgcttt gagattgttt
     1081 aaatcgttgt gatctagtac attcactaaa acaatccatt ggttcagttt aggatcaaac
     1141 atctgatcag caatcgttag tggttcccca tcagcacgaa ttccttcgag tggtggaaca
     1201 tcggtaaatg gaacagtcat aaccatatca tcaagcactt gtccagcata ctctccacct
     1261 gttcgtccgt atttccaaat atttttcatt tttattcctc ccctacccaa taattaccat
     1321 ggaatgtaaa ccattcattt gcagcaaatt cgctattcgt aataattgtt cctaagtctt
     1381 ttcgcacgta catcgctttt gatacactac ttgttcctga cggacctgta agcatgccat
     1441 gaacaatttt tgcgacaaat tcatctggga cttcatcatc aagtggtaca ccaaaagcat
     1501 aagctttttt gaactttact gtaccattta caataacttc ttttccgtgt ttaataaatg
     1561 ttgcataccc atcgataaac gattgactgt tgtctttagt taatgtataa acagtgcaat
     1621 cattttcact gatcacttta tctcctgcta tttgaatacc atcggcaaag tttttcaaac
     1681 cttcaactct ctgaggatca tacacattaa cagcatcatt caagcctttt tcagtatatt
     1741 caggtgtgac atcccaactg taatcattgg gattgttact gtctttcaag ccttcaccaa
     1801 agtatttaaa ctgactaata ttcggggttc gtgtgtcgcc tttttcgatc ttgagccagt
     1861 caatttgaca tgcacctgtt gttgattggg gatactggat aatagttaac cgtttggggt
     1921 agttaactcc aaccttctgc ggtgtaaaag tcagacccca tgtatcaact aacccttcta
     1981 ctggctctaa gttaccatat ctataatctc cactatcctc gttgtaaaca atgaacgttt
     2041 ggataggtgg tttggttgcc ttcatggtaa ttgtataggt ttgacctaaa acaaattctt
     2101 cttccatgtt agcttggtat aggctgtagg cgctagattt gattggaaac gtaacagact
     2161 tattagcaat attctcaccc aaaggcgctt tacctagcca gtaagggtca tcaagtaagt
     2221 ttggctgata tggggtggct gttgagcctt cttcgatttt gatgtcgtac aacttaaatc
     2281 cgccatttat tttatccctg tcaacaaagc taatactcat ataaaatcga tctaaatttg
     2341 tgatttgata attaacgtta gctgtacctt tgattgttat ttctttccct acatcatctg
     2401 ttgtaatatt tgtactattt gcttccaata atatcttttc tcctggtgat gtacgataca
     2461 ccaaacgtaa tttatcaata gctcctgtag ttccttcatc aaatcgaact ttcgcactca
     2521 gagtataggt tttcccacta gcaagctgag gtgtattaat acgcgtaaac ataataatgc
     2581 ttcctgtacc atcagaagta aaatgtagct tttcgttatc cgaagttagt gacccgtggt
     2641 aaccaacgtt aaaatcgctc gattttagtt tggacattaa attcggattc ccactataat
     2701 cataattccc gaagtcgatg ctgttactgt acatcttttt cagcttgccg aggtcgccga
     2761 tttgctgatt ggtttgattc atcttgtctt caactgcttt aagattgttt tgtgccgatg
     2821 taacgttttg agaaacggta tcaatcttag tttgagtagc ttgtagttgc tctgttacct
     2881 cgtcaacagt ttgatcaatt gcttgtgtgg ctccctctac aaattcattt atttgttggt
     2941 ttgccttttc aatcgtagaa tccacatttg ttaatgctgc gttaacagca tctgttgctt
     3001 cttgtttgac tccatcaagt aattgttgaa agtctgcaat aaagtagtct gctttatctt
     3061 gcgctagtcc gtcgattgct gaacgcttca tacggaaggt gaagcctaag ttatcacttg
     3121 ttgatccgtc tggatactcg atgtaaacat aagcttccac cataccgtta taagctttca
     3181 tcgcttctgg caagatatat acgacctgtc cattcaagaa gctttcagtg atgaggtttt
     3241 tcgtgataaa agggattggc tctttcgtga tcattccatc tacttcatca tagataaaca
     3301 tcaacaagcg caagttagct cctagcaagt ccgccggtgt gccgttctgt tgttcgacgt
     3361 tgaactctaa cgctatttga ttatcgtatg atttaaatac aagccccgtt gcttgcaaat
     3421 cgtagtcttt cggctgtgta ggtactttga tagagccttt tttgatgaca tgcgccatta
     3481 tttcacacct tcaatcttag ttattttcac gttgttgctc attgcgggat tggaaacatt
     3541 gccggatgaa atatctaaag ctctgccgtt tgacatcgtt atctttcgcg cttcgattgt
     3601 cagctcaaat tcgatgaacg tcactcccgc gtctccattc cataaattcg cttttgtaat
     3661 tcgcgcgtat ctttgacgtt tcacttcttc tacaaagtct ccgtcaccgt ctgtgtagtg
     3721 gatacgaagc gtttggtaac ggaaagtatc atctggcaga ttgactgatt gtccttttct
     3781 taattcgcct tcaaaaagaa cagaagtagt gtaaacgcgc cgccagccta acgagttata
     3841 accctctacc ggttcgctgt tatgtgtcat tttgatgtaa atttcgccgc tataacgagc
     3901 aaaagcgacg attaattttc tcgaatcgtg atccgcgaat accatcattt caacaaaact
     3961 gtcatcttca atcccgtcag ggttgtctgc gccccatccg gctttggttg cgtatttgcc
     4021 gggcggaatg tctaaaatgt tcggatattt ttgtggtagc ttataatcgc gtaaccaacg
     4081 cccacgcgct tgcataactg tttccggtgt agcaaagccc gcgttgcttt cagtagctag
     4141 gacgtgcgcg tcgtgatccg agccgtcgta atggatagcc gcttgacgca ataaccattt
     4201 cacagcctct tcataactca tttcttcaaa aatatttggt ttgctttgaa aatctaacat
     4261 ttaattgttc accgccttaa tagttataag tgaagtctct cgcgcgccct acgcctttgc
     4321 ttgtcacttt tcttgttgct gtgtcaactt ctattttata aaacgctaat tcatccggtg
     4381 tatcaacttg gctagctgta tgagagaagc cgacgtcgca aagaatcatt tttactgttc
     4441 caaacgcccc ttcgacttgt tcgtgccaat gcccgcagaa ataacctact acaacgccgg
     4501 cgccttttgt attcattgcg aaggtcttta atccgccgaa cgaaccgtca ttcggttgtc
     4561 ctaatttaga ccaatcgata gttaccggac taccagattt aaacccttct atcaacgtgc
     4621 taatcatgtt ttcattgcga acgggaaact tgctgagacc taaaggcgta tgtcctacta
     4681 acgccacatg atagtttctc ggaacgttca ctaaccattc tccgaaagcg ttaatttgtt
     4741 tggcactgaa agctcccggc gctgtatcgc tgtaaccgtc cacgtacttg tcgcctgtgc
     4801 cgccttcata aaaatcacaa gtatcgaaac ggtaaattgc tgcgttttta tctgggaaca
     4861 acacgccacc gtacaaaccg ttccaatact cttcgaagtc agcgttgcat aacattccct
     4921 ttcgcttgcg ccacgcgggg tcgaaacaag cgtcgtgatt ccctttgcag ataataaccg
     4981 gtttttcttg tcctgctacc gctgcgttag tgaagcgttt gagcgtgcct aacatggaat
     5041 gtcgcgcgct ccattcatca ataatgccta tatcgctgcc gagtgaacca agtccgccgt
     5101 cgatattgtc tccgccgtaa atcatcacat ctgttttgtt ccctaatttt tgaaattgag
     5161 ggattgcgcg ccaatgtctt aaataagaag catctttgta accgacgcca tctatacgca
     5221 agttgtgatt atctgcgtga atgtctgtaa tgaaagaaaa gttaaatttg ctgttattaa
     5281 cactgttgac aaccgtattc aaattgcgcg gcactaggtc aacgtatttc atcgtgtcgt
     5341 aatcaaaaaa gcgtgttgtt tctctaattt gtgagctgcc gactggcact tggtattttt
     5401 ggtttaaacg atcagctaat gattcgtagt cgcctttagc ttcattcaga atgttaatga
     5461 tcataccacc tggatcgata ttttccagta tttcacgatt atcttctaac cactgctccc
     5521 agtcattttt gccctgatcc atgtaatctt tgaattttct tagcaaatcc tcaaatgtcc
     5581 acacatagcc agagtcacgt aactggcttc tagatattcc agaaatgact cgataggtaa
     5641 aatcctgtgt gctaaattgt tcactccaag ttccatcacc attaagtgat cggaaactga
     5701 aatgtgcggt gttttcacca ccccattgcc agtcaggctc acttaaggtg taaacaagcc
     5761 ttgcttgcgc tggactgtat tcttgtactt tttgttcaac aggttggttt tcgccaaatt
     5821 ttgttgtatt aataaaaaac ggcactaggc cctcgaatgt ttttagtttg ccatgttcca
     5881 ccacttcaac aacgaacttt tgcgttaaaa catccccttg ccgaattcga accaaattta
     5941 ttccgttgtt tggttcggtg gtggatagga ccattttatg ctgcgtttct gccatgacta
     6001 tccctccttt agaaatcgat gtagtctctt gcattgtgga aatgacctga agaagatgga
     6061 taaaactcat ccataaattg aaaatgaaga tgttctccag tggatggtcc tgttgtcccc
     6121 atcagtccaa tttgctgacc agcagttacc ttctgacctt ttgagacgtc tactcggctt
     6181 tgatgcgcgt atcctgtata cattccatca gcgtgtttaa tcactgtcca atttccatac
     6241 cagtcaaagt aatttgcatc acctgcaaca atcacttcgc catccgctga agcaaaaata
     6301 ggtgtattag gatttccatt tacaaggtca ataccgttat gaaattcttg tgctcctgtg
     6361 attggagaag tgcgccagcc aaattcgctc gtcactgtga ttggatccgc aatcggcttc
     6421 atatatcctt ttgatacagg aatttccaaa tctttgaact tgtcatacca ttcttgtgac
     6481 catgccgtcc gttctggatg tggatcacgt ggacgttcaa agttagccac gaatgcttgt
     6541 gctgctgtgt tgatatcggt cagattcatg aattgtgtcc atgtgtaagg ataagcgcta
     6601 gttgcgatcc attgaccatt tggtgcatgc cacatcaaca atttaaactg tgccgtgatt
     6661 gtgtctggat catcactgat gccagccttt gtcattaaat tgatcatgta gacacgtccg
     6721 ctagttgcgc cagaagaatc ggtccattgc catacaccat aaccgaaccc tggtgcgcca
     6781 ttgccctcat cagcggttgg attagcatct gattctcctt gtgcatttcc aagtaaggct
     6841 gcagcagctt gttgtgtaaa accggcacct attgccgttg cccaaatttg ccaatatcgt
     6901 ttatcccgat cagtagttac ttctggtggg tattgtccgt tccaaccgtt atcgttccct
     6961 ccggaattgc ctcctccatt agtgtcaatt tttgtaccgt taacatagag ttcttttaca
     7021 tctagccgtc catctatggt tatatttcct tctgaaaaat tgccgtcacc ataaagatta
     7081 tatttgcgct tatcagcagt aacatctgct ggaatttgaa aaataggatt ccctcgatcg
     7141 ccaccgtccc cagcattaat ggaaaaaata tagtttggct ctttccagac agcaaagccg
     7201 ttaatccctt tagaaccata agtagataca attgagccca agcgttctcc atgtacatca
     7261 tctagtccag tagacttgac ttgtttttca aaagaaagtt ctcccccctc tgccactaat
     7321 tggaaatctt tatcgtcaaa tgtttttaaa gctacccctt gcacgaggat accagacaga
     7381 atgcctgcgc gaataaaatt agcattgaaa gttccatcca acgtccaagc ggtcgtgcta
     7441 tcaccattgt gtacatcttg aattgtttgc cactcacctt ttttacattg tttgaaagat
     7501 atccctgagt tattttggac cataaaaaag cgtgatctag gaatgttagg tccatccata
     7561 taaacagttt catagatttc tctactatca ctaacaccag cttcaattcc atttacccaa
     7621 taaatagaac cgccattatc tcctgcacct cgcataatgt catcttgata ttttccaatc
     7681 tctgtcgatt cgtaaaatgt cattttgcta gattctaaac tattaatatt attgacaata
     7741 gaagccgttt gttttctaac atctt
//
LOCUS       DASJST010000130         7709 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJST010000130 DASJST010000000
VERSION     DASJST010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.132"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..864
                     /locus_tag="U4B05_002335"
     CDS             <1..864
                     /locus_tag="U4B05_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2051451.1"
                     /translation="LYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVKLLVKR
                     KNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGTNQYSV
                     DSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNK
                     LDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKI
                     QLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELI
                     ELQISEGIDKQ"
     gene            940..1158
                     /locus_tag="U4B05_002336"
     CDS             940..1158
                     /locus_tag="U4B05_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051452.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1145..3823
                     /locus_tag="U4B05_002337"
     CDS             1145..3823
                     /locus_tag="U4B05_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051453.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3814..4011
                     /locus_tag="U4B05_002338"
     CDS             3814..4011
                     /locus_tag="U4B05_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051454.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            4015..4626
                     /gene="mobC"
                     /locus_tag="U4B05_002339"
     CDS             4015..4626
                     /gene="mobC"
                     /locus_tag="U4B05_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEN2051455.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4611..6002
                     /locus_tag="U4B05_002340"
     CDS             4611..6002
                     /locus_tag="U4B05_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2051456.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            6218..6532
                     /locus_tag="U4B05_002341"
     CDS             6218..6532
                     /locus_tag="U4B05_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051457.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6525..7019
                     /locus_tag="U4B05_002342"
     CDS             6525..7019
                     /locus_tag="U4B05_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051458.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            7087..7401
                     /locus_tag="U4B05_002343"
     CDS             7087..7401
                     /locus_tag="U4B05_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051459.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7500..>7709
                     /locus_tag="U4B05_002344"
     CDS             7500..>7709
                     /locus_tag="U4B05_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2051460.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2974 a         1037 c         1471 g         2227 t
ORIGIN      
        1 ttgtatgatg agaatcaaaa aatttttttg atatttgaaa tcaaaaatct cgtagaaatt
       61 tcgggaatgt caaaaaatac aatcttatca gcactagatg aacttcaaaa agttaaactt
      121 cttgtgaaaa gaaaaaatgg cttcaataaa agtaaaatgc aaatgttccc aaatttttat
      181 tatttgttaa agcctgttct gtgcaaagat gatgtttata aaattaataa gataacccat
      241 caagaaaata gacaaaaaaa cttgatagtt caaaatttga actatggaac aaatcaatat
      301 tctgttgata gttcaaaatt tgaactatgc gacacatcaa tgaaaacaag agacaaaaac
      361 aattttgctg atagttcaaa aaatgaacta gaattatata agtcttataa agatagtaaa
      421 gatattaaag aatcacataa tgatgatctt cttgaatcac tttcaactaa aaataataaa
      481 ttagatgaag aattgatcga aacatttatt gaagaaaatc ttcttaaaca aatatgggga
      541 gaaagtattg tgaattgctt aaaactggct agcaatagtg attatcgtca attcgacaac
      601 tggtacaaaa aattcaaata tgcgattaga tctgctgaaa aagaacaaaa aattcaactg
      661 aaaattatct atgaaatttg taataacaag tattttgtag atcatgttag agaacagcta
      721 tcaatgactt tacgtgactt gatacgaaga gcaaaaacag atcatcgtat caaacaaaaa
      781 gacgattata tctttgcttc tctaaaaaat aaagcgttag aactaattga attgcaaatc
      841 agcgaaggaa tagataagca ataagaatag aattgagtca aatgaaatgg aggggtatct
      901 ccacttcatt tgagaacttt gaatatagga ggggttattt tgattaaaaa tcgtttaata
      961 gatcaagttt ggtgtctaga tcaaaataca gatagtctcg aattaatcga gactatttgc
     1021 ttcaacacta tagttctcga tttaagagct gaaaatgata attgtgtaac tcatgtcata
     1081 attaatcaaa aaatggctca gaagctctct gagagcctca gaaaatgggc taagggaggt
     1141 aacgatgaaa ataattgaaa aaaagattgc caataaaaaa ataaattggt tggttgttaa
     1201 ttttcttgtt ctgatagcta tgattatttt tacaagtaat attgctcatg cgagtatttt
     1261 caattggttt gatgacacaa aaagtcaaac agagtttttg aataattctc tttatagacc
     1321 ttacctaaaa aagtacaatg gagatttcct tagtcagttc gggatttggc tcggatgggc
     1381 tgtggtaaaa ggcatgttta ctgtgacaga ttcgattcaa aatatgattc cagatgtgct
     1441 ggatttgttc aatttcattg aaagtacagg gctaaataat gtttatcaat cagccgtaaa
     1501 tacaattgtc attggattaa tgatcctttc attgatgttt gttggttaca aaatgattac
     1561 tggaaaagga acagtggatt taaaatcggt tggaatgaac gtagtgatgt ctatagcatt
     1621 aatactttta atgccaacca tgatttctag tggtattcaa tttgctaaaa cattttataa
     1681 ggactctact acgattacaa attcagatga cggagtcgct tggtcattga taaaacaagg
     1741 agtaacagat ttagcatata ttaataaaac agatcagtac tcaagcattg ataaaacgga
     1801 ggatcgaaat aatttaacac gaaaaaattt tcaacagacg gatctcacac aagtgttaac
     1861 ggataaagtg attgataaat tggaaaaaga aaatccaatt gcggacaatc ttcgttatga
     1921 attagttgaa aattctaata atgaatttgt tgctacaaag ttttctgaca acttcttatc
     1981 aacattttct gatagcttga aatcaggcta ttaccgatac caagcaaatc tatgggggat
     2041 ttcaattggg ttgacagcgt tagcaatcgc ctatgtgttc agcgcgtttg tgatcattac
     2101 agcaatacta gaacttgcat ttaaacgtgt gttaggtgtt cttgtttttg caacagatat
     2161 tgagacagga cagcgttcaa aagttgttct atcagatatt ctacaatgtt acttaactgt
     2221 tgggtttcaa ggatttgggt tatcaatgtt tgctatgttt atcaattttc tgaactcagg
     2281 acaaggtatc tcaacaaata tttttataaa aacaattgct tatatctgtg ccgtttttgt
     2341 tttaatcaaa ggcagtggaa cagtcatgag atactttggt gttgatattg gactcaaaga
     2401 aggctatggg caacttgctt ctgcttttgg aatgggcgca atgctgttta gaaaaggatc
     2461 aaatggattt aatcgtgcta aaggaagcgg agaaggagaa gatcgaaaac cagaaaagaa
     2521 tttcggcgaa actctatcaa aaaaagctgg caaaactgga agagctttgg gatatgccca
     2581 tgaacgtgga ttatccggtt tagcttcaga tggagctaca atggctagtg aaagagcaac
     2641 aaaaccattt aagagtatgc gtgatatagc taatgatacc aagaataagt ttaaagaagg
     2701 tttagatgat ggtacagtgt ccgctattaa taaaaatagc aaacctatgt tagctaaaaa
     2761 taaagaagat gaaactggaa aatatgctga ttccgtgcca acacgtcttt cagatagaaa
     2821 agatggtgct aaagttgaaa atgccgatag aattatgtct agttctgaac gtatgcgtga
     2881 agcaatgaaa aataatgctg aatcgaataa taatcctgca agcgcaattc agcaaaaagt
     2941 ccaacaagat attgaagaac gtaaaaatgc tatgcacggt caagctaagt ctgctgaaga
     3001 attaattaat cagaagcgtc atgaagcgaa atacacgcct gaagcaatga atcgcgagga
     3061 aataatgtta aggaaacgtg tagaagggcg gacaggcgct aatattgacg gaaaagaagc
     3121 gttgaccaaa gaaagaattc aagaagctaa aaattcaaat acaggattag aaaaattagt
     3181 caaagaaaat ccacaaaatt ctacaagtag tcatggtggc cgatcagtag atgtccgaga
     3241 aaatattcaa ggttcactga atggtcatgt tggacggaat gttgatgtaa gagagaattt
     3301 acaaagttct tcaaatgctc aaggtactaa gacaattgat gttcgagaaa atctgcaaag
     3361 tagcagtaca agtcaaccaa aaacagtaga tgttcgagaa aatgtacaaa attctacaag
     3421 tagtcaaggg tttaaatcag tggatgtccg agagaatgtg caaggtagca gtgcaagtca
     3481 accaaaaaca gtagatgtcc gagaaaatgt acaaagttct acaagtagtc aaggattcaa
     3541 aacagtagat gttcgggaga acattcaaaa agatagtgga gaaatgaaag aaaaaacaca
     3601 aaaaatcaat atcgttgaag acagaaagtc agcgaaaaaa tttgattcaa atcatgaaac
     3661 agtgattatt gatagtgaaa tccgtgaaaa tgataaggga tcaaaatcaa gacgaagatt
     3721 cacttatgaa gacaatgagt tattcagaga tacacttaat gatcctaatc cacttttcga
     3781 taatttatta agaaataaaa gggggggatt gtcatgcaat taattctaaa gattagtacg
     3841 catgatttct tagaacttga cgaattatgt gaatcagtaa agaacgcaac aaatgaaaaa
     3901 atcacggtca ctaattatat tgaactgatg attcaagaag aattaaagat gaaaaatgaa
     3961 atagagagca atttcaaatg ttgctctcta gaaaaaaatg tgaggggata atcaatggat
     4021 gataaacaaa aaatgtacga aaaaaatcgc aaacatagtg aggccttaaa ttttcgtgtc
     4081 gataaaaaga cttatcttga actgacattg attgcaaaac aaaggggaat ttctagagca
     4141 gacgtttgcc gtgagcggtt aaagggactc aaatatgaac cgccgttgtt agctgaagat
     4201 gatagggtag ttttaaccaa acagttaaat caaattggta acaatttgaa tcaagcggta
     4261 aaggcattaa atatcattgc taaatatttt agagaagaat ctgaagataa aaaagaaaag
     4321 agcctacatt ttagttttga tgctagccgt tacaacattt ttaaagatga acttgcgaat
     4381 agcatagcaa aaaagttgat gattagttat ccagatgaca tttttttgga gtccaatcgg
     4441 agtgccccct ctttttttaa attatcagaa gtagaaaaag aatatttcga atattggata
     4501 gaaaagcaaa ctagaccggg tacgaaagaa gagtattccc aaaaagttga tcggaattta
     4561 attcactcaa ttcagattat tgcaagttta aaagatgagg tgaaacaact ttgggagcta
     4621 ctataacatc gaaaggacgc aagaaaaatt tatcctcgct attacaatat gcagctaatc
     4681 cttcaaaaaa agaaaaatgt gttgcagtct caggaataaa ttgtagtgat gatagtgagg
     4741 aagcactgca agaaatgaat ttagtacgta ccaagatgaa taaatctaaa gatatagcac
     4801 aagcgtttca tgttattcat tcgtttgata agagtacttc taaagagtta agtattgata
     4861 aaatgcacga aatagctgtc gaatttgcag agaaagcttt tccaaattcg caaatcattg
     4921 ttgcaagtca caatgataaa gatcattttc atagtcatct tgtcattaat aatattaaca
     4981 tggaaacatg taaaaggttg cgtattgacc ctaaagattt agaactaatg cgaaaaatta
     5041 acgatcaagt cctcaatgaa catggtcttg aatcagtgaa taatgagtac tatgaaaact
     5101 tagaaaagag aaccaatctt tattcaaacg atccttctaa gatgaaatat ggaaaaatta
     5161 attatcaaga gtttgtgcaa aatgcgatcg aagcaacgtt ggaaaaatgc gaagtgaatt
     5221 cttttgaaga tttttcggat catttggcaa aagaatataa cattgaagtt tatcaatttt
     5281 caaaaacaaa taacaaactt ggctatgttc tttacaaaca aggtgtaaat ttctatgagc
     5341 gtagaggtga gttaattact ttaggaagag ataaacaatc tagaaaagaa aaaagtcagt
     5401 atgtcgaacg tacttttagt gcaagaaagt tagggaaaag atattcattt gaatctgttt
     5461 ctgaacaact tgaaaagaat ttagaagaat cacatataaa aaattttttt aatatggaaa
     5521 gagagcaagt tatgtcgcta attagtggag taaattacaa aactggtgag ttaaatgtac
     5581 cgtttgtgga attagattcc atcgcacaaa agcaatttag cgaccgtgaa ggcgatgaaa
     5641 aattcattaa atatgatgac ttcttgcgag aggtagacaa gcattttgta aatacaatgt
     5701 ctttagaaga tgatcaagaa tacacgatta aacttcatac ggatgtgaat cctcaaactt
     5761 tacgtagtgc gaaaagtaaa acaatttcta ttaaaaagac tgatcagaaa gagttatttc
     5821 taccggcaaa gggttttgaa aattgttctc atacaccttc tgctaaacag attcactatc
     5881 aaatcagtcg cataatgaat gacgatgtaa aaatgtatcg tgctaaaaag gattatgaat
     5941 tcacacaaca aatgattgaa aatcagcaaa aaaggcaaca aaattttgaa atggaattct
     6001 gaatgggctt gttagtggac tttccagttt tttgcctatg aagagttgct tggaaagaag
     6061 cagtttttag gacatacgcc agctacccat ctacttgcca gtcaatccgc cccgctcacg
     6121 ctaggctact ttctggcaag tcagatggcg ggtaaatctc tgagggagca agctccctac
     6181 agactccctc ccaaaaaaag aaaggtggaa gaaaaaaatg aactatccat attttaaagt
     6241 ctcggcttca gaagaaacta aagaaatatt caacaatttt tacaaccaaa acaaaggaat
     6301 ttttggttca aaggcaaata tgtttcgtgt tatggtttca aatttaccag tgttagctag
     6361 tccatcaaat aataaattta atgatcctga aagcattaaa tttgaacaaa aaatttcaga
     6421 actagaatca atgataagta atgaagtaat tgaaaagctg gatgacattg atcaaaagtt
     6481 atcgtattct ctaaaaaaca aatacaaaac ggaggagaaa aaagatgttt aaagtttact
     6541 tcccgtttca gtggctcgtt aaaggaattc ttttagtgct actaggattg attctttttg
     6601 gaagttatct aacaattaaa gccagacatt ttccagaaga aaagccaata aaaattgtaa
     6661 caaaagattt aaaaataggt gaaacttatc aggcagatgg ggttgaacta acatttaaaa
     6721 atattgaagt gaccgaaaaa agaaatgaat ttgcacaaca tgatcgtgta ttacaagtga
     6781 cctatacact tataaatgat acagaaaatc cgatacgtcc acaacaatgg tcgattgaaa
     6841 ataaggatgg tgaagaactt tatgagtatg atatgatatt ggataatcca gagtatattg
     6901 aaagtggcaa agaagaaaca gtaaattact attatgaatt accgattgaa aggcaggact
     6961 accaaatcgt agtacgtaga gagaatggtt acatagttgg gttgccattt actttataag
     7021 ttttattttg ttagtagttt ttaaggtaat atcagttttg tgatttttga aaatggaact
     7081 gggagaatgg ctgaggaaaa taaagttgtt ccgtttgaaa cattagtgat aaataaggaa
     7141 gagtttgagc aactttttct tgattttttt attcaatatg acatgattta tgattttagt
     7201 tcgtcactag atttatttga attagaagca gaaataatag aaaacagtcg agagaagaat
     7261 ggtaaaatca ttatttcagt agaaaaattc aatgaattat tctcacaaac atttgaaata
     7321 gatccagact attatcaacc acaagaagtt tttcgagagt ttagaaataa gattattggt
     7381 tttagtcaaa ctacaaaatg aaagcttaat aaagcaagtg ttgatagaat tattcctcaa
     7441 cgcttgcttt attttatggg cacttcataa tagaggtata gagaagagag gtattcattt
     7501 tggatactat tactccaaca aacttcaaag aaaatatagg taaagtgttg caagaagtag
     7561 ttcattcaaa ttctgaaata gaaattacta gggattctaa aaatggattg aacgatggcg
     7621 tcattatgat ttctaagaga aaatgggagt tgttacaaga agaactttat ttacaacgaa
     7681 cgggtacatg ggcttatgta tctgattta
//
LOCUS       DASJST010000131         7683 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJST010000131 DASJST010000000
VERSION     DASJST010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.133"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..201)
                     /locus_tag="U4B05_002345"
     CDS             complement(<1..201)
                     /locus_tag="U4B05_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN2051461.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEK"
     gene            complement(198..716)
                     /gene="msrA"
                     /locus_tag="U4B05_002346"
     CDS             complement(198..716)
                     /gene="msrA"
                     /locus_tag="U4B05_002346"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2051462.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(736..1449)
                     /locus_tag="U4B05_002347"
     CDS             complement(736..1449)
                     /locus_tag="U4B05_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN2051463.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1452..2348)
                     /locus_tag="U4B05_002348"
     CDS             complement(1452..2348)
                     /locus_tag="U4B05_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2051464.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2506..3348)
                     /locus_tag="U4B05_002349"
     CDS             complement(2506..3348)
                     /locus_tag="U4B05_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2051465.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3480..4133
                     /locus_tag="U4B05_002350"
     CDS             3480..4133
                     /locus_tag="U4B05_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN2051466.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4196..4723)
                     /locus_tag="U4B05_002351"
     CDS             complement(4196..4723)
                     /locus_tag="U4B05_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2051467.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4743..5690)
                     /locus_tag="U4B05_002352"
     CDS             complement(4743..5690)
                     /locus_tag="U4B05_002352"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN2051468.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5702..7588)
                     /locus_tag="U4B05_002353"
     CDS             complement(5702..7588)
                     /locus_tag="U4B05_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2051469.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         1991 a         1575 c         1351 g         2766 t
ORIGIN      
        1 tttttcccat gcttcgtcaa agatatccat ggacggcaaa tagtcgacat tcagttccag
       61 ataattagaa atttcttcgt agctagagct gtgcttagga aattggatgt ctttaccggc
      121 ttctgtagcg aaaacagata aagcatcatg tgtgccaccg cctcgcagtg tcattaaata
      181 atgaaaaaag cttcgattca cccttcgttc ctccaatttt cttcgataaa ggcagcccgg
      241 cgtgcatggt ttcttttaaa attttcagga tgctttttat aatactcttg atggtcttct
      301 tctgctagat aaaatggttg ggctggttcg atttttgtaa cgattggctg ttcgaaacgt
      361 ccactttcag ctaactgttt cttacttttt tcagcaatga tccgctgttc ttctgtcaaa
      421 taaaagatca ccgggcgata attgtcaccg cgatcctcga attgtccgaa agcgtcagtt
      481 ggatcagttt gctgccagta aagttctact aattgttcat atgaaatcaa tgttggatca
      541 tagataatct ccactgcttc tgtatgtcca gtcgtttggc tttttacttg ttcataagtc
      601 ggatctggta catgccctcc agtatatcct gacatcactg tatggatacc aggaagcgtg
      661 tcgaaaggct ggatcataca ccagaagcag cctcctgcaa atattgctcg ttccattatg
      721 attccccctc taatactact catccgatga ttttttattt tcttttgtct tgctttcagt
      781 actgctggtg ctagttttgt tggtatttga gttgtttgaa gcatcagagg tatcttttgg
      841 caaatataga ctaaaccgaa tgtcatcatt tactaagtcg attttatctg ctttaatata
      901 catcccgttt tgcattcgga attgatccaa cctaagcatg atagtcagat catctgtatt
      961 gacttcgatc caatctggta atttatagtt tctcttgatc atattcatca catctttgat
     1021 tggcaaactc aatgttccga tagaaagact tctagctttt agttggacat ttccgttttc
     1081 catcacatac ggatcaaaat ataaatagaa atcgatagga aaacctagta cttcgaaagt
     1141 accattgagt agtgcttcat tttccaaata aaatttatat tcaatatcac tatctttttg
     1201 atactcacta aggaaaaagc tgatgatttg attgactttt tctttatttg aattgatcgt
     1261 taagacagga tctccttgtc tttccgtaag agctggtatt tctgggagtt ctggttcccg
     1321 attagcaaaa atacggctgc ctaaaaatac ggtcgaacca agtactaccc caagaaggat
     1381 aaggaaggcg aatttccagt aatttgtttt ctttgttttg tttgaagata cgtcagatct
     1441 tgttttcatg tttattgatc atcctccttc tctaaccatt ggttttgtgt ctcgatcagc
     1501 tgatctctca ctgcatttgc catcaattga taacctaaat tatttggatg gaaatgatct
     1561 tcttcataga gcacattatt tttgatgcca tttagatttt ctgtcgtgct agttgtttga
     1621 tctgttcctt cttctgtgac acctacttcc tgattcaatc cttgataaag caaatcattg
     1681 attgggataa agtagcagtt cggcattttt tttgtcaccg tttctgttcc tttgttccaa
     1741 ttgttaacaa ttgtctgcat gtcagtgatc tcaggaaagt tcaaataaaa tggattgtag
     1801 atgcctaaaa tgtaaatagg tgcggtttta ttgagggatc gaaattcttc caatagttct
     1861 gtgacatttt cctgatagtt tttcagtggc tttttaaagg aattgaccgt taacccgaat
     1921 aaattatttt ggataacttt catcaaatca ttaccgccaa cagtcagtgt gataaaatca
     1981 gctgttttga tattgtttcg aatttcttct tttgttttta ctcgctttaa gatctggtca
     2041 cttcgttcac catttacacc atagttttct gtttcaaccg cggtcaaatt ataacggtct
     2101 tttagatcat ttgcgacaag gggaacgaat cctccttgct tggtttgatc tcctactcct
     2161 tcagtcagcg aatctccgat agctatgtag tgaagttttt cttttttatt tggatcttct
     2221 ctcacagtag ttactggttt cattagtggg ccagctttcg gtatagaagc agctaaacct
     2281 gcatagatga tgatcgcaat taataatggg aacaaaacga ggatcaaatg tcgtttcgtt
     2341 aatttcattt tttcacctct tgtattcgat tggttctaat gttatcaaaa attttatcaa
     2401 aaaggtagtc tttatgaaaa aagaaaacaa ttattttcct aattttataa agaaaaagag
     2461 aataagctgc aagcctttca ggctgagggc ttatcctctt ttcgactaat ctacataata
     2521 catgattgca aacgcgtctt taccagtatg cgtagcgata accggattcg tatgcagtac
     2581 agggatatgc atgtccggga ataattcttg caaaccctct ttgaattctt gcgccaattc
     2641 ttttgcatcc gcatgtgaaa taccgatttg tcgcacattt ggcaattctt tcagttcatt
     2701 tttcaaatta ttaaaccatt tatcgaacgt tttctttcct cgccccttag ttacagggat
     2761 taattcagaa tgggcgaaat tcatcaccac gcgtatgttc aaaacactag acagcaaacc
     2821 agttgttcgg ctgattcttc caccttttac caagttatct agtgtggata ttccgatgta
     2881 taattttgta ttgtcttgta cttttttgac ttcttcaagg attttctcta ctgttttacc
     2941 ttcttttgct aattcagctg ctcgaattac ctgaaaagat aatccttggt cagtggtatc
     3001 actgtcgata accgttactt tcgtttttgt tagattactg gcttgtcttg ctgcttctac
     3061 tgttccactc aagccttttg tcatatggat cgaaatcact tcactgccgt cttctcctag
     3121 ctcatcatac atcgagatga attctccaat tggtggttgg cttgttttgg gcagattttt
     3181 tgctttcgcc atcatttcca taaatctttc gccgtctagt tggtcatcat cgggatatac
     3241 tgttccgtcg atcatgatgg atagcggcat gacacgaatg cctaaagcat cgcgtacatt
     3301 tttttccatc gtacatgaag aatctgttac gatttttaca tttgtcatta ttttctctct
     3361 ctctttcttt aacaagatgc cttattcatg ttaaactaca tgtaagacaa tttctatttt
     3421 tagtataaca gacaaataat gatttttcat taattacacc tagtaaagga gccgttttca
     3481 tggaaaaaac gaaatttaat cgaacgtatg aaatattaaa tgaagtgttt aatgcagtca
     3541 cccatggaat tgggactgga ttgagtatcg cgggactagt ggttctttta gtaaaaggtg
     3601 cccacttgca ttcctccctc catattgtct cttatgctat ctatggatcg atgatgattc
     3661 ttttattttt aacgtctact ttgtttcatt cattgatttt tacaaaagca aaaaaggtgt
     3721 tccaagtatt cgatcatagt tcgatttttc ttttgattgc aggaagttat actccttttt
     3781 gtttgctgag tatccgagga tggctaggtt ggactttatt tgtattaatc tggttgtttg
     3841 cgattcttgg cattatatat aaagcgttga cgttgcataa acgggagaga gtatcaaaag
     3901 tatctacgat catctatatt tttatggggt ggctctgtgt cgtggctgct gttcctcttt
     3961 atcactctct tggaatgact ggtgttattt tgatggtagc aggcgggctt tcttacacga
     4021 taggcgcatt cttttatagc ttgaattcta tccgctatat gcatgttgtt tggcatttgt
     4081 ttgttatgtt aggtgcaggt tttatgtact tctctgtttt attctttact taataaaaaa
     4141 aataaagcta tggatgggac agctctaaat aacagctagt ctatccatag cttttttata
     4201 tcgtcaattc gcctgtcccg ctttttacag gattctcatt acgctgatac gtttcaaatt
     4261 gatgcggata acgatttttt tcatccattt ctccttgact agtattgatc agtgaccaat
     4321 cagaccagtc aacaggtgga aaataagcat ctccatcgaa tacatgatgg atgactgtac
     4381 ggtataatat cgtacaaaat ggcagaaatt cttgataaac agctgaccct ccagcgatga
     4441 aaaagatacc gtctgtttct ttttccagag caagcacgtc ttccacacta tgcatgacat
     4501 gcacgccttc tgctttatag gaagaatctc tcgtcaaaac aatcgtctga cgattcggca
     4561 acggacggct acccatccct tcgaatgttt ttcgtcccat cactagtgta tttgcttctg
     4621 tcatttgttt gaagaatttc agatcatttg gcaaacgcca aggaagacgg tcttcttttc
     4681 caatcaatcc gttttcatcc tgtgcccaaa ttgcagctaa cattttctca tcctttctga
     4741 ttctaaacag cgataggagc tttgatcgcc ggatgtgggt catatccttc taattggata
     4801 tcttccatct caaaatcaaa tactgactgc ttttcctgat tcaaaactaa agtcggaaat
     4861 gcgcgtattt ctcggctcaa ttgttctttc atttgttcga tatgattgat ataaagatga
     4921 gcatctccta atgtgtgaac aaattcgcca accgctaaac ctgtttcatg tgcaattagg
     4981 tgagtcagca acgcataact tgcaatgttg aacggaacgc ctagaaacac atccccactg
     5041 cgttgataca attggcagct tagccgtccc tcttgaacat aaaattgaaa catcgtatga
     5101 caaggaggca aagccatgct aggaacatct tccggattcc atgcagaaac gatcaatctt
     5161 ctagaatcag gagtcttttt gattgcttcg atcacttgtt tgatttgatc gataaagcca
     5221 ccgtcttttg tttcccagtg acgccattga tagccgtaga tgtgtccaag atcgccgtat
     5281 gtttcagcaa aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct ctgcttttta
     5341 tattcttcat tgaattcagg atctgtcaaa catctatggc caaaatcagt catatctggt
     5401 ccattatagt ctggactttt gatgtagcgt tcaaatgccc attcatccca aatatggttt
     5461 ttattttgta atagatagcg gatattcgta tctcctttta aaaaccaaag caattcgctt
     5521 ttgatcaatc caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt ttgcagatca
     5581 aaccgcattt ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc ttctttacga
     5641 tgcccttctt ccaacaattt tcttcctaag tcaagatacg cttgttccat cctttttcct
     5701 cctaatcttc tacccattca ctcaagtatt cccaccgtgc cattttttct tcaagttgtg
     5761 cttctgtttc agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta tctccttgat
     5821 gattcatctc ttcttggagc aatgagattt tttcttcaag ttctgcaatc tcatcttcaa
     5881 tcgtttccca ttctttttgt tccatgtaag ataatttctt cttttcagca ggttccttgc
     5941 tctgtatttt tggtttcgct ttttcagcag gctgctgggt ctctttttgc ttcagtaaat
     6001 aactggacat gtttccgtaa taaagttctt gttttccgtt tccttggaat actaataact
     6061 tatctgctac cttgtccaag aaataacggt catgggaaac agtgatcaca gcacctggaa
     6121 aacttctaaa ataatcttcc aaaatcgtca atgttgctat gtccaaatca ttggtcggtt
     6181 catccaataa taaaacatta ggttgctgaa tcagcagttt caacaaaaat aaccggcgtt
     6241 tttctccgcc tgaaagtttg cctatcagtg tcccatgcat aaatcttgga aataaaaatc
     6301 gttcaagtaa ttctgctact ccgatttgac tgccatctgc tgtttttgct tcttcagcag
     6361 cttcttgtaa gtaagcaatc atccgtttat ctggtgccat ttcttcattt tcttgtgtat
     6421 aatacgcaag atgaaccgtt tcaccgatag acattgtacc gctatccagg ggaattctgc
     6481 cagctagaat attaagcaat gtcgattttc ccgcaccatt tttaccagta atacctagtc
     6541 tttcccgaga ttgaatcaat aaatctaaat gttctagcag tgtttggtca tttatacggt
     6601 aactgccatc tttgatttcc agtacttttt tccctaaacg ttgtgtcgct aggttcagct
     6661 ctaaatcatc ttcttggttt acttggtaca agttctcctt tagatcttcg aaacggttga
     6721 tccgtgcttg ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa gctaattcct
     6781 gtttataaag ccgcttgcgc ttttcttctt gttctacttc tacacgatca cgttctgctt
     6841 tttctaagac gtaagcttca taatttcctt tatactcgta taattttcca aatgaaagtt
     6901 cgaagattcg gtttgctact ctatctagaa aataacgatc gtgggtaacc atcaatagtg
     6961 cgccacggta ttgtttcaaa tagttttcca accactcgat cgcttcataa tctaaatggt
     7021 ttgttggttc gtctagtaat agtaaatcgg gttcatcaat caacacttgc gccagactaa
     7081 ctcgtttttt ctgaccacct gataaagtac tgattttttt atctaattca ctgattccta
     7141 atttttgcaa aataatcttt gcgttggtat ctgtagtcca agcatcttct ttattcattt
     7201 gttcttctgc tttagcataa cgtttttgca catcagggtc ttctccgttt tcccctagtt
     7261 cgatcaatgc ttcctcatat gctcgaacag tttggatcaa ggggctgttt ccttgaaaga
     7321 ctgcttggag aacagttaat tcatcggaaa attcagctgc ttgagaaaga taaccaatac
     7381 ggtaatcagt tggataaaac actgtttgcc gatcaccgtc gctgctatcg ataccggcaa
     7441 taatatttaa gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg atacggtctt
     7501 tttcgtggac caaaaaagaa atctggtcaa acaaatcttt ttcaccataa gtttttttga
     7561 ggtcagttac ttttaattct ttcaacatgt tccctcctta tcttggatat tgtatcataa
     7621 aaatagtgaa cttcaggaaa atttttttgg tatttgttct ttttgtgttc tcaaaaaaag
     7681 aac
//
LOCUS       DASJST010000132         7645 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJST010000132 DASJST010000000
VERSION     DASJST010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.134"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..603)
                     /locus_tag="U4B05_002354"
     CDS             complement(<1..603)
                     /locus_tag="U4B05_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2051470.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRI"
     gene            complement(603..1739)
                     /locus_tag="U4B05_002355"
     CDS             complement(603..1739)
                     /locus_tag="U4B05_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2051471.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1771..3222)
                     /locus_tag="U4B05_002356"
     CDS             complement(1771..3222)
                     /locus_tag="U4B05_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2051472.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3266..4030)
                     /locus_tag="U4B05_002357"
     CDS             complement(3266..4030)
                     /locus_tag="U4B05_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN2051473.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4058..4756)
                     /locus_tag="U4B05_002358"
     CDS             complement(4058..4756)
                     /locus_tag="U4B05_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN2051474.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4768..5547)
                     /locus_tag="U4B05_002359"
     CDS             complement(4768..5547)
                     /locus_tag="U4B05_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN2051475.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5563..6507)
                     /locus_tag="U4B05_002360"
     CDS             complement(5563..6507)
                     /locus_tag="U4B05_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2051476.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6553..7332)
                     /locus_tag="U4B05_002361"
     CDS             complement(6553..7332)
                     /locus_tag="U4B05_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051477.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2154 a         1614 c         1170 g         2707 t
ORIGIN      
        1 aattcttcca atatgtccca ttacaaacgt ctgacttgaa attcctaatt tcattcgtac
       61 atcatttcta atactttcat cataataaaa ttttttagca tccactgcat ttggaatcaa
      121 agtgaattta tcatttcgaa acaaaaaatc acctgctact tttccacatg aaaattcatg
      181 tgtcaaaaca gacgttaact tacatcgata atattgcttt aaaagatatt taaaatctct
      241 atctatcgat gtattatggc tatgtgctat ccgtatcgga atcccagctt tttttgcagc
      301 ttttaaaggt aaataactca tcgcatcaat atgagaatga acaatctcat attctggatg
      361 atccttaaaa aattgttcca tccatttaaa atatttagga taattttgtg gataaagtct
      421 cggtgcataa tagacctttc ctccaagact tattatttcc tcatcataat cacttttata
      481 tggacgatgt gtcaaaaaat caaactgaat tttagttcga tctatattcc tataatagtt
      541 cattagcatc gtctccaacc ctgcacgatg catatcattc acacactgta atattcgaat
      601 cattatatat ttcccctata caaatttaat gtttcttgta cgattttatc ccaattatat
      661 ttactacaaa taaactcagt agcttgattc ttcaaaactt taaccatttc cgattgattg
      721 catgcctctt ctaatcttat ctttaaatca cttacatccg atttcttaaa aatcatagcc
      781 ttgtcttcta ctacttccgt acactctgag atatttgaaa ctatacaaca gttcccataa
      841 ctcattgctt ctaacaaact taatggcatt ccttctaaat cacttggaag tgtatagata
      901 tacgcattac tatataattc ttctaattcc tgtccttgta caaatccagt aaatataatt
      961 cgctcatcac cttttgctaa ttcctttagt tcatttgcaa attcatctgt atcagagctt
     1021 cctccggcta ttataagttt tttatctgtc tgaacatctt taaatgcctc aattagatac
     1081 cgaattcctt tttcgggaac caatcgcccc aaaaacaaaa tataagaatc ttttgttaat
     1141 ccatactttt ctgtaatcaa tcctgctgat tttatttcaa tttttttaac gccatttggt
     1201 ataaaccttg tttttcttcc atatatatct tcaaaataat tttggacgct ttcactaagt
     1261 acaattattt catctgcaaa ttttacagca cacttttccc ctaacatgat gtatgtacta
     1321 gcaagcttgt tccatttttc tctttgatga tctagaccat gaattgttgc aacacatctt
     1381 ttcccaaaaa gttttggcaa ccacaacata gcacatggtc cttctgcatg aaaatgaaca
     1441 atatcatatt ttccgaatgc tgctctaatt ccaccaaata ctgaagaact cattgctgca
     1501 attcctttta tatttaatgt aaaaatagtc tttaatttta cacctttata ttctttattt
     1561 ttatgctggt caaattcctt tccgctaaca tgatgaccac ttcgattata gcaagtcact
     1621 tcatgcccaa gtgcaaccat tcgcgttgcc aattcttcta ccactatctc aattccacct
     1681 tctcttgatg gaattctttt atgacctagc atacagattt ttaatttttt tcttgccatc
     1741 acgttttact ccttctatag tatctttatc ttacatggaa ccgtttctat gaagcacagt
     1801 tttgactgtc ctaaacaaaa ttctaaaatc taatcccata ctccaattcc tgatatattc
     1861 tgcatcaagt tttacaacct cttcgaagtc tgtgatatca cttctaccac tcacctgcca
     1921 cattccagta atcccaggtt tcatcgcaat tctcgcacga tgatgaaaat catacttctc
     1981 atattctgac ttcaagcaag gtctaaaccc caccaggctc atttttccca ttaggacatt
     2041 ataaaattgt ggaaactcat ccaatgatgt agatcgaata aattgtccaa ttcctgtctt
     2101 ctttgttcca tcaggaagta ttttattgcc aatgattctt gggtcaaaat ccatcttaaa
     2161 catgagatta ttactcatct tattttcttt catcaactct gctttgcgtt cttctgcatc
     2221 catatacata ctacggaact tatacatctt aaatgtctta ccattttgac caatacgttc
     2281 ttgtgcaaag aaaatcggcc ctggtgattt gatgtaaata actggtgcta cgaagataaa
     2341 aataacacct gttgcaatac atccaataat tccagcaaga atgtcaatcg ttctctttat
     2401 aaatgcttgc ctcatcgtca taacattaat actagatgta ataacagtat aatcaccaac
     2461 tcggccaata aattgttttc cacgtgtact atcagacact tttgtaagat tcatatgtac
     2521 agcaagtccc atttccgaac atctggcgat taattctttg ggatatggaa cactttcgtc
     2581 aacattgatg aatacttcat ctacccaatt ctgtaaaaga tattccgctg cattatcagc
     2641 atttgcaact actgggacac ctccgatggt agttccatac atatctctgt ctactacaat
     2701 cacaccacta agtttataca tgccataatt cttctcttta agattacgaa ctacattttt
     2761 tgcaatcttt tgagatgtta caataactaa cgatacatct tcaccgtttt tcatgcggtg
     2821 atgcaacaac cctttccaaa gtatacgaat tatatatgtt aataatccat acaccacccc
     2881 cattataaat aaagaaactc tggaataatc atttccatcc tgcattgtaa taaggtataa
     2941 actgcacaca agtaacagca taaaagattg ctttatagaa gctacagcct ctttataata
     3001 tcctcttttt aaaacatttt tatatgcctc aaataaaaat ataatcgtaa gattctcaag
     3061 ttctataaaa attgccatgt ggcgataaat aggaatcaca tacggattcc agtcaccctg
     3121 tcgcattaca tatgatatat aaaaagcaag ctggacacaa aataagtcta ataaaataaa
     3181 atcaatatgt ttagaccaac cacttgagtt ttttctatac atctctcact ctctcctcat
     3241 ttccttttta tctacgaata aagaactaaa ataaaccaaa cttcttcttt tttacctctt
     3301 catagattgg ataattgatc tcatccccgt tgatcagatc ctttgcgact tgtttcatct
     3361 gtactacttt ttccttacca aactccttgg caatctgctc atagcactct tttaagtaaa
     3421 aggtgcgctt agagaccccg tgtgcatcag aagcagccat ctgcacgaga ttatgtttga
     3481 ccattgtttt ggctgttttc tggatatctt tgccaaatac accaacataa cttggcgcag
     3541 taagttgggc caaacatccc atatctagga acggaagcaa atgatttggg tcttctcgaa
     3601 aatgcgcgtt gcgttctgga tggacgatca ctggtatctt tcctagttct aacaactcaa
     3661 aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc agaagatatg
     3721 tatcatctaa atctgtaaat aaaatttcat ctcggtggat gtcttcgatg agcgttccgg
     3781 taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc tctaattcgg
     3841 cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat
     3901 tgtggtgtgg tgtgcataaa atatgcgtaa tgccttgctg aatcgctttt tcagccattg
     3961 caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata tggcaatgta
     4021 gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag taataaccag
     4081 catctttgct gtgttctgct ccgttgtata cgacacctaa gactcgtgct tgtaccatat
     4141 ctaacagttc ttttgcttta gtcaatgatt cttttcgtga cgtattttcc cgtacgacca
     4201 ataaggtgcc atccgttttt gaagacatga tctgtgcatc tgttacggca acgactggcg
     4261 gcatgtcgaa gatcactaca tcaaatagtt gtcttgcttc tgctaaaatc tgatccattc
     4321 ttggcgaact tagtagttct gatggatttg gtggtttagg tccacttggc agaatcgaca
     4381 aattgtcgac tggtgttcgt tggatctcat ctgctacact tcctgatgaa ctaagaacag
     4441 tacttaatcc gcttgcgttg ttcaacttga acgttttata gatgacgggc ttacgtaagt
     4501 ctgcatcgac tagcagcacc cgttgaccag atttggcaaa aacaacggca atgtttgctg
     4561 cagtggtcga tttcccttct ccaggtcctg atgaagtcac aacaatcgtt ttgatttgct
     4621 gaccagctgc ggaggcaaac tggatattcg tccgaatcgt acgatattgt tcagcgattg
     4681 gtgaagaggg attggtgagt gtgaccaaac tgactgcatg agtttgggct gctttttgtt
     4741 tctgtgttcg tgccattttc tttcctccta gactcttgag cggcttctgc gtgatggcgc
     4801 tgttttatcg tttgtagctt ttggttgtgt ctgagctggt ccttttttac ggataccagc
     4861 attcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc ctaatgtatc
     4921 tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga atgccagacc
     4981 tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat tatttggcga
     5041 aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa cattcatcac
     5101 atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt gagcagccac
     5161 tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct gattaactga
     5221 gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt ctaaacgatc
     5281 tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat tgatcatttg
     5341 caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg tcacgatcaa
     5401 ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc
     5461 caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg tttcaaaaat
     5521 ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat tctctagatt
     5581 catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga ctttttgata
     5641 ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa tcgtaccaaa
     5701 tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt ctgttttcat
     5761 attcgactct agagcattca aaacggattg atagttgctg acaccgttta gactcaatac
     5821 ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt aatcaccatt
     5881 tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca atgtaatttt
     5941 accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga cttcgatacc
     6001 accaactgca tcaacgagtt cctttagccc tttcatattg atagagacat agtgatcgat
     6061 tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc cgaatgcgta
     6121 agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg tatcacgagg
     6181 aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca tcatcgtatc
     6241 tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca atagaacaga
     6301 gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg ttcgttccac
     6361 tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg ctcctactcc
     6421 tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata tttttttccc
     6481 tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt gcttgaatca
     6541 acgttttaac tgtcaatagc ttatattgct atagcccatt tgctcagcaa cagtttgtgc
     6601 ttttgttgcg tatgctgcgc ttattttttc attccggatc tgctctattt gactgagcac
     6661 cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttta acatcgtatc
     6721 gaataactga tctagttgcg tgacttgcgc gatctgaata tcagcgaatc ctagatattg
     6781 cataacaacc gttttccaag gactatcttt aaaaaagctc atattttcac gtacacgagc
     6841 gacatctgca cttgtcacct tttctttgat gatgacatcg tctacagctt gctgccagtt
     6901 tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt ttgattctgc
     6961 actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag cttctttagg
     7021 caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg atagcttatt
     7081 tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaaattt tttcttttgt
     7141 actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt gattggtgat
     7201 ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga tatggtatcc
     7261 aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg acttgacgaa
     7321 tggcttttcc atagattcac tcctaagtat tgatgataga taaattaaga tataattcac
     7381 ttacataaaa atgacagctt tttatttaaa aaaacataat taaactaata tattcacaga
     7441 aatatttgta aacaagtttt ccctatataa tcataccaca aactgttttc ttgactaggg
     7501 caatttacga tttaaaagta aataaaacat taataagata aaaattaatc gatttcattt
     7561 ttatttccat aattttcata ggtgttttta aaagttacac aaaaaataga ataacaaaaa
     7621 aattaacatt gaattaataa tagtt
//
LOCUS       DASJST010000133         7612 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJST010000133 DASJST010000000
VERSION     DASJST010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.135"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="U4B05_002362"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="U4B05_002362"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN2051478.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="U4B05_002363"
     CDS             complement(2032..3285)
                     /locus_tag="U4B05_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2051479.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="U4B05_002364"
     CDS             complement(3291..4214)
                     /locus_tag="U4B05_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2051480.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="U4B05_002365"
     CDS             complement(4435..6927)
                     /locus_tag="U4B05_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN2051481.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="U4B05_002366"
     CDS             complement(6934..7401)
                     /locus_tag="U4B05_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN2051482.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="U4B05_002367"
     tRNA            complement(7537..7610)
                     /locus_tag="U4B05_002367"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
BASE COUNT         2095 a         1661 c         1226 g         2630 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc at
//
LOCUS       DASJST010000134         7589 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJST010000134 DASJST010000000
VERSION     DASJST010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.136"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(931..1344)
                     /locus_tag="U4B05_002368"
     CDS             complement(931..1344)
                     /locus_tag="U4B05_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN2051483.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1800..2285)
                     /locus_tag="U4B05_002369"
     CDS             complement(1800..2285)
                     /locus_tag="U4B05_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051484.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2424..2639)
                     /locus_tag="U4B05_002370"
     CDS             complement(2424..2639)
                     /locus_tag="U4B05_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN2051485.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2863..3663
                     /locus_tag="U4B05_002371"
     CDS             2863..3663
                     /locus_tag="U4B05_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2051486.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3682..5550
                     /locus_tag="U4B05_002372"
     CDS             3682..5550
                     /locus_tag="U4B05_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2051487.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5543..6034
                     /locus_tag="U4B05_002373"
     CDS             5543..6034
                     /locus_tag="U4B05_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN2051488.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6022..6576
                     /gene="srlA"
                     /locus_tag="U4B05_002374"
     CDS             6022..6576
                     /gene="srlA"
                     /locus_tag="U4B05_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN2051489.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6594..7589
                     /locus_tag="U4B05_002375"
     CDS             6594..7589
                     /locus_tag="U4B05_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN2051490.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1212 c         1441 g         2230 t
ORIGIN      
        1 aataacataa taagaaagta aagtggattg atttgagatc tttttatact tcaaaaaaga
       61 taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta atacatttta
      121 atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag agagatcata
      181 agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc ccatatttct
      241 tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat ccagcaccat
      301 ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg aagtaaaaac
      361 aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc ccccacattc
      421 aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt atgaataaca
      481 gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga agtttatttt
      541 tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa gccgatttcg
      601 tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt atggataaaa
      661 tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa taagtaaaag
      721 aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa gacaatcaaa
      781 tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt aaaaacaaaa
      841 tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat atataaaaat
      901 cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt ttgtttcttt
      961 tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa aggttccagt
     1021 gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga ccgaatgtct
     1081 ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat gattggcgga
     1141 gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac agccaggcat
     1201 atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg gataacttga
     1261 aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag ggaacatgtc
     1321 gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt ttttatttat
     1381 ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt agctttgtga
     1441 atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa
     1501 taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa
     1561 aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa
     1621 gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa
     1681 tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta taactttctc
     1741 agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat
     1801 caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa
     1861 aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa
     1921 taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa
     1981 cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca
     2041 gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta
     2101 tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta
     2161 gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt
     2221 ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta
     2281 tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa
     2341 aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata
     2401 acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg
     2461 aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa
     2521 atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac
     2581 agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt
     2641 agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat
     2701 ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca
     2761 ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct
     2821 tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat
     2881 attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc
     2941 gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata
     3001 cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga catagaagaa
     3061 gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa taatgcagga
     3121 ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca
     3181 gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa
     3241 gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa
     3301 tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac
     3361 agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt
     3421 gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct
     3481 tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc
     3541 cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca
     3601 gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga
     3661 taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta
     3721 ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat
     3781 caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc
     3841 atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc
     3901 atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat
     3961 ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa
     4021 gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat
     4081 gattttgatg taaagattac aggcacacca aataaaggac taagaataat aggagatgag
     4141 ctagaattac gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca
     4201 gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt
     4261 aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt
     4321 aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa
     4381 ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa
     4441 aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa
     4501 gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat
     4561 ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg
     4621 ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa
     4681 aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg
     4741 caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt
     4801 cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa
     4861 ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata
     4921 acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt
     4981 acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt
     5041 tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg
     5101 tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta tcaagaatat
     5161 ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga atttgaaaaa
     5221 aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt tgcttttcct
     5281 cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat aactgatcaa
     5341 ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc tgaagaaatg
     5401 acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga actcataaaa
     5461 atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt aaaactaatc
     5521 aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt tcgcagcttc
     5581 agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca atcaaacata
     5641 tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga aaatccgttc
     5701 aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag tcagaaaaat
     5761 gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg gtgaagatat
     5821 ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga cacaagcagc
     5881 tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa aaagtgaaca
     5941 accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa gtacgatcaa
     6001 agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg atttatgagg
     6061 ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt accagttgtc
     6121 ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga ccgagtaacg
     6181 aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt accgttctta
     6241 tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct tccagaatac
     6301 tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa tggtgtattc
     6361 ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg gatcgaaacc
     6421 ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact gctgatgaac
     6481 tttattggtg gttggataac tgattttact actggatttg tatgcagaca gcaaggaatc
     6541 gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt taaatgtatg
     6601 gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt attaggccga
     6661 caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca atgatcgtag
     6721 ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca tcaatacctg
     6781 atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt ggaatttatc
     6841 ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc ccattagcac
     6901 aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata cagccactaa
     6961 tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa aagtacaaat
     7021 atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg atagcaaaaa
     7081 ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga gaagcagtag
     7141 atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg attggtgtga
     7201 tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta gcaggaaatg
     7261 cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct cctttgcttg
     7321 gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag attgggaaag
     7381 gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact caaaatgcat
     7441 gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca gtagaagtag
     7501 gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt atcattgctt
     7561 gggtagcaag ttttggatta tatagctga
//
LOCUS       DASJST010000135         7493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJST010000135 DASJST010000000
VERSION     DASJST010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.137"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..1524
                     /locus_tag="U4B05_002376"
     CDS             133..1524
                     /locus_tag="U4B05_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051491.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1597..2244)
                     /locus_tag="U4B05_002377"
     CDS             complement(1597..2244)
                     /locus_tag="U4B05_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2051492.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2252..2938)
                     /locus_tag="U4B05_002378"
     CDS             complement(2252..2938)
                     /locus_tag="U4B05_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2051493.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2944..4017)
                     /locus_tag="U4B05_002379"
     CDS             complement(2944..4017)
                     /locus_tag="U4B05_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2051494.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4239..4913
                     /locus_tag="U4B05_002380"
     CDS             4239..4913
                     /locus_tag="U4B05_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2051495.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4918..6258
                     /locus_tag="U4B05_002381"
     CDS             4918..6258
                     /locus_tag="U4B05_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2051496.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6407..7393)
                     /locus_tag="U4B05_002382"
     CDS             complement(6407..7393)
                     /locus_tag="U4B05_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN2051497.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2324 a         1386 c         1316 g         2467 t
ORIGIN      
        1 atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga
       61 gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaatta
      121 aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact
      181 aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa
      241 gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat
      301 cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt
      361 tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat
      421 tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat
      481 caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc
      541 cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga
      601 agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat
      661 aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg
      721 tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga
      781 gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc
      841 ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt
      901 ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg
      961 tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa
     1021 ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg
     1081 atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga
     1141 tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta
     1201 tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat
     1261 aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat
     1321 atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat
     1381 cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg
     1441 aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa
     1501 ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat
     1561 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
     1621 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
     1681 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
     1741 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
     1801 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
     1861 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
     1921 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
     1981 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
     2041 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
     2101 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
     2161 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
     2221 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
     2281 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
     2341 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
     2401 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
     2461 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
     2521 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     2581 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     2641 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     2701 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     2761 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     2821 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     2881 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     2941 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     3001 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     3061 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     3121 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     3181 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     3241 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     3301 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     3361 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     3421 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     3481 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     3541 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     3601 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     3661 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     3721 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     3781 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     3841 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     3901 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     3961 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     4021 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     4081 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     4141 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     4201 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     4261 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     4321 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     4381 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     4441 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     4501 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     4561 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     4621 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     4681 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     4741 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     4801 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     4861 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     4921 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     4981 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     5041 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     5101 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     5161 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     5221 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     5281 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     5341 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     5401 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     5461 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     5521 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     5581 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     5641 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     5701 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     5761 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     5821 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     5881 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     5941 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     6001 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     6061 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     6121 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     6181 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     6241 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     6301 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     6361 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     6421 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     6481 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     6541 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     6601 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     6661 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     6721 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     6781 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     6841 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     6901 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     6961 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     7021 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     7081 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     7141 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     7201 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     7261 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     7321 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     7381 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
     7441 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aat
//
LOCUS       DASJST010000136         7281 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJST010000136 DASJST010000000
VERSION     DASJST010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.138"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            300..569
                     /gene="rpsN"
                     /locus_tag="U4B05_002383"
     CDS             300..569
                     /gene="rpsN"
                     /locus_tag="U4B05_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2051498.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            584..763
                     /gene="rpmF"
                     /locus_tag="U4B05_002384"
     CDS             584..763
                     /gene="rpmF"
                     /locus_tag="U4B05_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2051499.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            795..1172
                     /locus_tag="U4B05_002385"
     CDS             795..1172
                     /locus_tag="U4B05_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051500.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1193..1342
                     /gene="rpmG"
                     /locus_tag="U4B05_002386"
     CDS             1193..1342
                     /gene="rpmG"
                     /locus_tag="U4B05_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2051501.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1366..2349
                     /locus_tag="U4B05_002387"
     CDS             1366..2349
                     /locus_tag="U4B05_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN2051502.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2379..2501
                     /locus_tag="U4B05_002388"
     CDS             2379..2501
                     /locus_tag="U4B05_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2051503.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2519..4042
                     /locus_tag="U4B05_002389"
     CDS             2519..4042
                     /locus_tag="U4B05_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN2051504.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4066..4305
                     /locus_tag="U4B05_002390"
     CDS             4066..4305
                     /locus_tag="U4B05_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051505.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4498..5133)
                     /locus_tag="U4B05_002391"
     CDS             complement(4498..5133)
                     /locus_tag="U4B05_002391"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2051506.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5154..5372)
                     /locus_tag="U4B05_002392"
     CDS             complement(5154..5372)
                     /locus_tag="U4B05_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051507.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5498..6702
                     /locus_tag="U4B05_002393"
                     /pseudo
     CDS             5498..6702
                     /locus_tag="U4B05_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            6697..7104
                     /locus_tag="U4B05_002394"
     CDS             6697..7104
                     /locus_tag="U4B05_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2051508.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2566 a         1086 c         1429 g         2200 t
ORIGIN      
        1 tttctttcat attgccgttt taagtagagg aaaagctact taaaacggta tttgtgtttg
       61 attggactaa taaaagtgaa ttcttatttt tcagagctta aactgcatat tcttttaaat
      121 gatttgatta tctctatgta ccgataaatt cgttttcaaa tagttatcta ttcatatgag
      181 ttttatttca tgttatttat tgttgacaag agcaactctt gttggtaaag tatcagtgtc
      241 aaaacgtaac gattacgatt tgagatgtct aaatcaaaat aaaaggagta taaaggtata
      301 tggcaaaaaa gtcgaaaatt gctaaagcaa agaaacaaat ggcgatgatt gaaaagtacg
      361 cagataaacg tcaggaatta aaagccgcag gagatcgcac cgctctcgct aaattgccaa
      421 gagattcaaa tccgaatcgt ctgaggcttc gtgatcagac ggatggtcgt ccaagagggt
      481 atatgcgaaa gttcggtatg tcccgtatta agtttagaga acttgcccat caagggttga
      541 ttcctggagt aaaaaaagca agttggtaaa aggaggaaat atcatggcag taccagcaag
      601 aaaaacatct aaggcgaaga aacggttaag aagaactcat caaactgtag taaaaccaga
      661 aatttcattt gacgaagcga atggtgatta tagacgtagc catcatgtat ctttaaaagg
      721 gtactataat ggaaagaaag taattaaagg agcttcaaaa tagtttaata tataactttt
      781 aggagggaat caaaatggaa attatttacc cacctttagt tgaagaagga ttaaagtatt
      841 atcttgaaac gacccagcaa tcgctagata aatgcacttt ttatcgttcg atggtagaga
      901 gaggaattat tactgaaacg ggtttaccga cccaacaggc aattgaaaat ggattagtca
      961 aagattacta tgaggaccaa ggcttatcat ttgatgattt tttaagaatc tatccaattt
     1021 ttgaagagta tgacgaggag ttatttcaat gtattgatgg ttattgggag atacctgttg
     1081 atatgaaaga aaatttagtt tcacaattgg aatctggcga gttaactttt gaagatgcac
     1141 aacaaattca agcatatctt gaagatcgat aagtattgaa ggaggaagat ttatgcgtca
     1201 aaatattatt ttggaatgtg tcgaaactgg agaacgtctg tatctcacca gcaaaaataa
     1261 acggaataac cctgaacggt tagaattaaa aaagtattcg ccaaaattgc gcagacgggc
     1321 aattttcaaa gaagtgaaat agctaagtgg taaaaaggtg acaaaatggg cataccagtt
     1381 acaatcgttt ctgggttttt aggggctgga aaaacaacgc taatcaatca agccttgcaa
     1441 gtctcaccct accccaggga agaaattatt ataattgaaa atgagtttgg tgaagttggg
     1501 attgatcaca aattattagt tcatagcgag gaacgggtgt tacaactaaa taatggatgt
     1561 atgtgttgca gtcttcgcag tgatttatta gcaatattga tttctttact ggaaacaata
     1621 gaagaacatc aacaaccaat ttcacaaatc atcattgaaa ctacaggcat agcagatccc
     1681 caaccaatca ttcaaacact tttaacagcg cctcaactaa gaggaagagt atatatcgat
     1741 agtttattga ctgttattga tagtgacagt tttgaactta ttgaaagaga accacaagcc
     1801 cttaagcaac ttgtcatagc agatcgcatt tttgtgtcta gaaaacaaaa cttgaattta
     1861 tctgcactaa aaaaattaca gagagaaatc aaaagtatta accctttatc cgatattcga
     1921 atgttcacac atagtgaaac gattcaaaaa aaggattttt ataatttgga aaaattcaat
     1981 catcccttag tttttgaaag tgattcatca gaaaatcact ataatggcaa acactatcat
     2041 caacatcatc aagaacatga gtttaaatca attctcttaa cttcagatca agacataaaa
     2101 aaagattttt tattccaatt aattgaccgg ctgattttaa ccaaccaagg gagcctgtat
     2161 cgttttaaag gattgattgc tttgcaagat caagatttta tagttgccat acaaggggta
     2221 aacgaacagg ttaattttca atacaccact cgacaaaaag accaaaccac tattattcta
     2281 attggaaaaa tgttggatga agaaaggatc aaacagagtt ttgataaatt actggcggaa
     2341 agcaaataat gataagtatt gattaggggt aatttctaat ggagaaaacg gatatatcta
     2401 gtgcatatcg acgacttaaa agcccgaata tcaaaactag aaaaagggca ttaaaaatta
     2461 tcaaagaaca taaacaaaat aaaatgaaga aattagcata attaaggaag gaaaaagaat
     2521 gaagaaaatc agtgtaggac ttattgggat agcagctcta ggattattgg gagcttgctc
     2581 cagcactaat gatgcaaaag tatcgaatga taaagatgga aaattagaaa ttgtaacaac
     2641 attctatcca atgtatgatt tcaccaaaaa catcgtcggc gatgaagcga acgtggattt
     2701 aatggttcca gcgggatcag aacctcatga ctatgagccc tcagctaagg atatggcaaa
     2761 agctcatgat gctgatgttt ttgtatatca taacgaaaac atggaatcat gggtaccaaa
     2821 agctaaagaa agttggaaga aagctgggcc taatgtagta gaaggaacaa aagacatgat
     2881 tttattgcca ggtagtgaag aagaagatca tgatcacgga gaagaagatc accatcatga
     2941 actagatcct catacgtggg tttcacccaa aatggcaatt aaggaagttt caaatatcaa
     3001 ggatcagtta gttaaattgt atccgaaaaa agcaaaagta tttgagacaa atgctgaaaa
     3061 gtatttgact aaactgaaac ggttggatgc tgactatacc actagtctta aagaagcgaa
     3121 acaaaaaagt tttgtgacac aacatgcagc ctttggatat ttagctttgg attatggatt
     3181 gatacaagtt cctattgctg gactaagtcc tgaagaagag ccatcttctg gtcgattagc
     3241 tgaattaaaa gagtatgtta aaaaaaataa gatcaattat atttactttg aaaagaatgc
     3301 taatgataaa atcgcaaaaa ctttggccaa tgaagctggg ataaaattgg aagtattgaa
     3361 tcctcttgaa agtcttacaa aagaacaaat ggataatggt gaagattatg tttcagtaat
     3421 ggaagacaat ctaaaagcgt tagagaaaac aactatggtt gctggtgaag aggtagttcc
     3481 agaaaaagaa gcgaaggatg aaaagacagt tgctaatggg tactttaaag atgccgatgt
     3541 gaaggatcgc gaattgagtg attatacagg cgaatggcaa tctgtttatc cgttgttaaa
     3601 agatggaatt ttggatgaag ttttcgatta caaagcaaaa ctcaataaag acatgacagc
     3661 tgccgagtac aaagattatt atactactgg ctataagaca gatattgata ctatcaacat
     3721 taaagacaac accattgatt ttgtagtcaa cggtgaacat catcaataca catacaaata
     3781 tgttggatac aaaattttga attatgaaaa aggaaaccgt ggtgttcgtt ttaattttga
     3841 aactgatgat gcaggtgctg gtcgttttaa atatattcaa tttagcgatc atggaattgc
     3901 accaagtaag gcagagcatt tccatatttt ctttggtgga gaaagtcaag aaaaacttta
     3961 taatgaaatg cataattggc ctactttcta tccagcttca ttatctgagc atgagattgc
     4021 tcaagaaatg atggcccatt aaaatccaat ctttggagga aatacatggt aagaaaagta
     4081 ttattgtcta tggattccaa gaatattgat gcttatcggc gttatcaatt tgcaaagcaa
     4141 atgagattaa atgggagtac attagctgat attgctgcaa ctttacatgt tagtattgac
     4201 acagttcata gagtattaaa acgggatcct gattgttgtt ttatcaaggt caatactgga
     4261 agaagtagac gtttagcaaa acggcataat agcttggcca attaattgat aataccagag
     4321 tagatcattt ttcccttatt tggatttgat ctgcttttta ttatttcttg aattgtcaaa
     4381 tcaagcgcaa cactttatca aagaagactt cataaggtgt tctccaatct aaacactttc
     4441 taggtctgtt attcagtttt tcaatcatct gatttatcgt tgagttatct accgtagcta
     4501 tctctttcga ttttggcaag tattctcgaa tgagtctatt cgtattttaa tttgttcatt
     4561 gttgccaagg gcatgaggat ctggaaaata aaagggcata ttattcaata cttctgtaac
     4621 tgcgaaatga ttggagaatt cttttcctct atctggagtg atcgttttga ccgcctcatt
     4681 tggaatactg cttaaaagtt caatcatttt ctcgctcaca taagcggctt tctttctggg
     4741 gaccttcttt cctgctaaaa ggaagcatga ttttctgtct gtcaaagtca ctaaacaagt
     4801 accactagtt ttgccagcta caatatcggc tttccaatga ccaaaatcac ttctatcatc
     4861 tgcggatttt ggatggccat ggatcgaatg agtaaggcaa atcttcccac gagactcgac
     4921 atgatttttt gtatgtcttg tttttcctcg atggtgcaat gaacggatca ggcctttatt
     4981 gccgtgtgag aggggttctg tatcgaaaag atcattgcaa attgcacggt agatcgtgat
     5041 aaaactaatt gaaaagtcca tatgttcatg cttcaaacga ttcgagattt tctctggcga
     5101 ccactgaaaa tcgacaaata aatgacgaac aattgaccaa actcacggat catttagaat
     5161 gtgataacgt ccgcattttt ttcttcgata agcataattt ttttccgctt tggaaggtga
     5221 ttagtttttt tcttgtttct tctcagttca tgtaaaatag taaacgggct atgctgaatg
     5281 tcgttgccaa ttatactaat agaaaatcct tcttcgtgaa gcagaaagat tctttctcgt
     5341 ttgagtatag taagatgaca atactggctc ataattattt tccttctagg agtgttctcg
     5401 acaaacttat actagttgga tatcgattat gactcatttt tatgtgttgc acttaaagtg
     5461 taaattcaag gtatagaaag aggttcccta tgaaaaaata cctattacaa tcttaactgg
     5521 ctatcttggt tcaggaaaaa cgatacttct aaatcatatt ctgtacggtg atcacggaaa
     5581 ggaaattgct gtcattgtaa atgacatagg ggaagtaaat gttgatgggg tattagtcga
     5641 caagaaaggt tttaaaagaa ctggagaaaa acttgtttcg atgccgaatg gatgcatttg
     5701 ttgtacatta cgcgaagatt tgattgttga actagatcaa ttagtgcaat tttctgactt
     5761 gaatcaaatt gttattgaag cttcaggaat cagcaaacca attccaatta ttcaagcaat
     5821 tatattagct aaaaaccctt tagacattga tttgacccaa cgattagaga ttggtgccat
     5881 tattatagta gtggatgtaa aacgtttttg ggataatttc gaaacgggtg aaaagattca
     5941 ggaaagaaaa gctgaaggta atgtagttga agaagaacag gatattcgtg gcttgttaat
     6001 tgatcaattc gagtgttgta acatattact tttaaataat agtgacttag tgtcaaccaa
     6061 gacgatttac aaaattaacg gtctaattgc taagttacag tccaatgcca aaataattag
     6121 gacaattaaa ggtgaagtag acttatcgaa aattctaaat acaaatcttt ttaatataga
     6181 tgagacttcc tattcaatgg gctggttcaa agagttagag cttggctatc aaaatcatat
     6241 tcctgaaaca gaagaatatg gtatctcaag ttttatctat cgaaatcggt tactccttga
     6301 cgcacaaaaa tttgcccatc ttacggataa aaattttcct ggcaatatta caagagcaaa
     6361 agggtttctt tggtttgccc aacatagtga tttttcgacg atctttgaaa catcgggtag
     6421 ccataaaagt attgaatcat taaattattg ggtagctagt ttacctaaaa ttgagtgtca
     6481 aatgattata caaaaaaacc cgaattgaag gaagcctggc atccgttgtt tggtgaacgc
     6541 attaataaac tagtttttat tggtatgaag atggatcaac aatgtattaa ggaacaactt
     6601 gagggttgtt taactgattc tcttaatgca gataatttcc gaaaaatttc accatttcca
     6661 tggaaaaatt ctaatatgtt tagccagata tcgttaatga aatttttaag aaaagctggt
     6721 gtcaagatag gcgatgtcat aattattggc attttgatta ttccctcttt tattccctta
     6781 tttgtattct ctatagaaaa ggagaatgaa acaactgaag atgctatcaa atatgccatc
     6841 gttcaaattg atggcaagga aatggatcgc ttcgatttgg ataagatcaa tcataaactc
     6901 gtgaattatc atcctgcgaa gggacaatat aatattgttg agattaaaga tggaagaatt
     6961 cgagttaaag aagataatag tcctgatcaa atcgcggtta gaactggatg gatctcaaaa
     7021 cccggtcaaa cgagtatttg tttaccccat aagttagtta ttagtattga gaaaaaagaa
     7081 agcaaagatt actacattta ctaatctgga aatagatgag gaggttttta ataagaaaaa
     7141 gatattatta ttaataatag caggggcttt attaagtgtg ttaagcgctt gtggacaaga
     7201 aaaagaagcg gtgacaacag gcacaagtat ctttaacggg acctgcaatg gtttagttaa
     7261 cagtcggtta gattacttat t
//
LOCUS       DASJST010000137         7103 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJST010000137 DASJST010000000
VERSION     DASJST010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.139"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..454)
                     /locus_tag="U4B05_002395"
     CDS             complement(140..454)
                     /locus_tag="U4B05_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN2051509.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(468..728)
                     /locus_tag="U4B05_002396"
     CDS             complement(468..728)
                     /locus_tag="U4B05_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN2051510.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            898..1212
                     /locus_tag="U4B05_002397"
     CDS             898..1212
                     /locus_tag="U4B05_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2051511.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1214..2869
                     /locus_tag="U4B05_002398"
     CDS             1214..2869
                     /locus_tag="U4B05_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2051512.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2952..5087
                     /locus_tag="U4B05_002399"
     CDS             2952..5087
                     /locus_tag="U4B05_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2051513.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5262..5771
                     /locus_tag="U4B05_002400"
     CDS             5262..5771
                     /locus_tag="U4B05_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051514.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            5768..7072
                     /locus_tag="U4B05_002401"
     CDS             5768..7072
                     /locus_tag="U4B05_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2051515.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         2370 a         1213 c         1463 g         2057 t
ORIGIN      
        1 tttttgtttg gctgttctta ttacttgatg caggacagcg ttttcacaac ctttgattca
       61 cggttttcca gaagtagctt cttcgaaaaa taagccgaaa tttcttgaag ctgatcactt
      121 ctgtcgcaac ctctactgac taaggcttga tataagcata accttgggat tgcagttcaa
      181 ctaaatcaag gacacctgct ggtacaacga ttacttgttc aggtaaagaa ctttctgaaa
      241 tatgattagc acgcatagca ttggcgcaag cgtgaaaaac gactccttgc aaatctaaga
      301 attctgcatt ggcagagtct agataaccgg taatcgcgat gccatttaca gatacgacaa
      361 tcgtcgtttc tggagaagct tttatcaggt tttttacgtt tttccctgtt tctgaccatt
      421 tttctaattc atcaatatgg aaaacgactt tcattgaaat cattccttta ctttttaata
      481 atcatcttga tcgcttgttc tagtttagcc gcttgttctt ctggattact atcaggtttc
      541 tcaaagcaat ttttcagatt atctgcaacg atcatgccca ttacgcgatc aatactagaa
      601 cgaacagcgc ttaattgagt gatgacatcc atacattctt tttcgtcttc gatcattttt
      661 tggataccac gaatttgtcc ctctgtacgt ttcaaacggt tcaggatatt tttattatca
      721 atctccataa cacattctcc tctattttta taattagata atacccctta tcggtatttt
      781 atgcaaatat aaaaagtagt gaaaaaaatg aagagtcatt ctggttgaca tattaataaa
      841 gctcctgttt aatatacccc atgaggtatt tataaaaatg taaatgaggt gcagaaaatg
      901 aatttgctat ttcaatcaat aaaatcgata acagtcccag aattaaagga aaagcttttg
      961 gaaaatccag cgttacttga tgttagaaca cctgcagaat accggggcgg gcatataaaa
     1021 ggagctaaaa atgttccatt acaaagtata aacagatatg atggagacaa agaaaagacc
     1081 gtctatgtga tttgtcaatc agggatgagg agtaaacaag cagctaaaga attaaagaaa
     1141 tctggttatg atgtagtgaa tgtacgtggt ggcatgaatc aatggtttga tcgaacagta
     1201 ggagggaaat aagatgaaaa tcgttattgt cggaggtgta gcaggtggaa tgtctgctgc
     1261 tacacggctt cgccgattaa tggaagatgc agaaatcgtt gtttttgaaa aaggaccgta
     1321 tgtttctttt gcaaattgcg gcttgcctta ttatctttcg ggagaaatca gtgaacggga
     1381 aaatcttctt gtccaaacgc cagaatcatt atccgctcgt ttttgtttag atgtgcgtcc
     1441 aaatcatgaa gtgacagcca tctttcccga aaacaaaacg gtagaagtcg tacatgaggg
     1501 tcaaaaacac attgaacagt acgatgcatt ggttttatct cctggtgcaa aaccagttgt
     1561 tccatcgatt ccagggataa cagaagccga caatgttttt tctattagaa atgtaccaga
     1621 tatcgataaa gtgatgcatg cattagaaaa acagccaaag cgtgccgtga tcgttggtgc
     1681 aggattcatc ggattggaaa tggcagaaaa cctaaaaaga agaggtttag aagtcatggt
     1741 gatcgaacaa gcaccacata ttcttccaac actggatgaa gaaatggcag cttttataga
     1801 aaaagaattg tctcatcaag gagtagaagt gattacttct catgctgtcg ctggatttga
     1861 agaccacggg aaacgattgc gactggatga tgggcgtacc atccctgctg atttagttat
     1921 tttatccatt ggtgttcgtc ctgataacca gctagcagtg accgctggaa tcgaattagg
     1981 catacgcggg ggtatcctag tagacgaacg ttatcaaacg aatattcctg atatttatgc
     2041 ggtgggggat gctatcgttg taaaacagca aatcactgga aaagatgcac ttatttctct
     2101 tgcttcacca gccaatcgtc aaggtagaca agttgcggac acgatttccg gaatttctcg
     2161 aagaaatcaa ggcggtattg gaacagcaat tatacgaacg tttggaatga ctgccgcatc
     2221 caccggttta agtgaaagaa cagccaaaga aaacgaactg tcttttgaag tcattcatgt
     2281 atcaggaaaa gatcatgcaa gctattatcc agaagcaaca gatattttac tgaagttgat
     2341 cttccatcca gagactggcg agatttatgg tgcacaaggt gttggggcaa aaggtgtgga
     2401 taaacggatc gatattttag caacagcaat caaagggcat ttgacgatct tcgatttacc
     2461 ggaattagaa ttgacgtatg caccgccatt tggctcagcc aaagatccag taaacatgct
     2521 aggatatgca gcaatgaaca ttgcagaagg gcttagtgaa accgtacaat ggcatgaatt
     2581 gccgacagaa ctagcaaaag gaaaaatttt attagatgtg cgaacagcag aagaattgga
     2641 aaagggcaaa ttcaaggaag ccaaacatat ccctttgaat gaacttcggg accgattaaa
     2701 tgaattagac agccaacaag aatatatcgt cagctgtcat agcggattac gtagctatct
     2761 agcggaaaga atcttgaagc agtctggcta ccacgtaaaa aaccttgatg gtgcattttc
     2821 tttatatcaa actgtccgac ctgaagaact gatatatcct aacaaatgat actcgttaaa
     2881 aagcttgcat tcgactctta aaagaggtaa actaatcggt tccaccactt taaaaaaaga
     2941 ggagaatgtg tatggtcaac acacaaaaac agttagaaga agcatatctg aagaacgtgt
     3001 atcaaaagct aattgataaa aaaaacgaac tgcgggaatc gatggatacg gcaaaaatag
     3061 aaggaaacca gtcgttgaaa gaaatggctg gcgagcttcg attgaatttt gacagttact
     3121 tagataacct ggatactttt tcgatgattg aaatgaaaaa tagagaaatc gatcagatga
     3181 atatcaaaca gaaaaacgca gaaaatcagc tagcaaagat cgaacgttta ttgaaaagtc
     3241 cctactttgg aaaagtcgtt gtagactttc tggataatga gccaaaagaa tccttttata
     3301 tcggtacagc aaatttcaca gatgatgcag atgaaaatct tgtttatgat tggcgttcac
     3361 caattgccga acttttttac aataacatga ttggtccatc ttcttatacg gttcgtcaaa
     3421 ataaaatcga tacggttatt aaagaaagac gacaatttct tttggaaaga gatcgtttgg
     3481 tcaagttttt tgatacgtct gtagctattc aagatgacgt attattagaa gtcttaggcc
     3541 aagatgagac caatgaaatg aaggctatca ctgctacgat ccaatcggaa caaaacacaa
     3601 tcattcggga tgtgaaaagt caaaatatct tggtaaatgg cgtagcagga agtggtaaaa
     3661 cctctgctat catgcagcgt attgcgtatc tgctctattt atatcgagat acgatgacat
     3721 ccgatgactt gctgattcta tcacctaatc atcggtttat cgactatatt tcgaatgtcc
     3781 taccatcatt aggtgaaaga aatccactga atctaaccat catacagctg gtttcacagc
     3841 tttcagcaga ggaaatagaa ggtgaagagg cttatttcaa acgtatcacg ggagaaaatg
     3901 tatcggagca gacagaacgt ttgcgtagca aaaagtttat cgataatcta aaacaatcag
     3961 atcctttgtt tttagatcat ccgaatttta tccgtggact tactaaaaat ggaaaaacag
     4021 tcctttctaa aaaaacgatc gaaaaaatat atgagaaagt tcctgctcat cctaaattga
     4081 tcgataggct tcaagcaacg aaaaaggcac tgatgagcga atggaagaat catcttttga
     4141 aacaagcaaa aagtcctgct gtgcagaatc aggtactctc gttgacagaa gataggcagt
     4201 tggaattatt cggtaaattg atttctgatg attcggaaca aagtattgta gcttatgcaa
     4261 ggaaactttt acaaaaaaaa tatcgaaaaa taaccagaca aatcgaagaa atggcatggg
     4321 tggatgagca tcagttgttt gaaagaattt atgaaaaacg atatggctca gcttatgctt
     4381 ggcaacccac acgtactgtt gatgaagcag tcattatatt agctattcgt catttacttg
     4441 ttgaaaaggt aaatgtacca gcatttcgct atctattgat tgatgaagta caagattata
     4501 ccctggcaca attaggtcta ttgatcgaat tatttccaaa aactcacttt actttagtag
     4561 gtgacgagaa tcaggcaatc tttaattctt ccactacttt tgctgatatt atgcgttgtt
     4621 tcgacgacta ccatctgccg atccatcggt atgatcttag aaatagttac cgttctagcg
     4681 gagcaatcac agagcttttc aaaacctatg ctgtggatca agagaagatc gacatcgtat
     4741 cgatccgacc gcaaggaaaa gaacccgaat accgctcttt tcgatcctct gaagaattgt
     4801 tgaaaatact agctgagatt ttggtttctc taaaaggaga aaaagcggca gttatcaccc
     4861 aaacagaaga ggaaacccaa acgctacaag aatcgattaa aaaaaattca tatggattag
     4921 aaaactgcca aatcattcct ttgagtctag caaaaggact ggaatttgat catgtaatct
     4981 tatatccatt tgaaaacgat ggagatgagc aaagaagacg cagacaaatg tacacggcga
     5041 tatctagagg aatgaaaagc attgtcgtat tggaaagagc aacataatta agagataaaa
     5101 aataaaaaga acaactgata aaaagaacaa aatatttttc atttgctgat gagaaatgtt
     5161 ttgttctttt tcttaggaaa aatttaggaa cactttatgt gaaaagaaat gaaatgtatg
     5221 gtaaactcct acgtcgtaac aaaaaaacgt gaggtgagga tatgaaaaaa gtaacaagag
     5281 aagaagttgc atcaattttg gtgaaagata agtatttttc aaaaggtaat tactggttaa
     5341 agatttggca aacgttagtg gctcttttag gatggatgat cgttgttctt ccttttatat
     5401 gggtcttttt cccattaacg agaccagaaa gagccaagga tttttccttg tacgcttttt
     5461 tatcagaaaa gaaaatgctg tatttcttaa tcggcttttt tgtattgatt tttttcagtt
     5521 tcctgattac ctcatttgta ttgactaggt ggaacaatcg aaatctatac aaaaaagtaa
     5581 ataaatcttt tgaatatgaa gatgaaaaat tgaaaaaaag acaagaatta cttgaagaag
     5641 tgtatacgga acgttttgga acaaaagaag agagagaaac ggtccgtttt tattctgtct
     5701 ccgaagagaa aaatctagat acgacattca ttgcagattt gtataaagaa aatggggtgg
     5761 aactgaaatg atctcaatca cccatacagg taatttattc ttagatactt gtttgtctat
     5821 tatgtatttt tttctcgttt cttatccgat tttaggcgga tttgtatggt ttattggtgt
     5881 ctggtgttat gtgtttttat acaaacataa gcaaaaagaa tgggtagatg ttcccttgag
     5941 tgtcgaaccg ttcatcacga tcatggtgcc agcccataat gaagaaattg tgatcgaata
     6001 tacaatcgag tatttgatga cgaaattgaa ttatcacaac tatgaggtat tagtgacaga
     6061 cgatggttca acggaccaaa caccagaaat attagctaga ctgatgaaaa aatacgctaa
     6121 tcttcgtgtg gttcggatcg aaaaaaataa aggaaaagcc cacgcgttta atatcggact
     6181 tgcctttgca aaagggaaat tgattttaag caatgatgca gacacggtac cggagccaga
     6241 tgcactgatt aggtatgtga attatttcat tcgccctggt gcaagacata ttgctgcggt
     6301 cactgcaaat atggatgtcc aaaatcgtac caaattgatt gcaaaatctc aaacggttga
     6361 attctcaagt atcgtgggca tcattaaacg aacacagtca gctgttttcg gtggtcttta
     6421 tgcgtacagt ggagcaaata cgatgtatcg aaaagaagcc ttgatcgatg tgggcggatt
     6481 cagacaagat cgagcgacag aagacatcag tattgcttgg gatcatcagt taaatgactg
     6541 gttatctgtt tttgctcctg agattatctt tttcatggaa gtacctgtga cgttgaagat
     6601 gctatatcga cacagaaaac gttgggcaaa aggtggaaca gaagtctggc tgacgaattt
     6661 caaaaaagtg atgctccatc cttttaaaca tattggaaga acgatcatct tcattgatca
     6721 aacattgagt attgtctggt caatattctt ttgtatttcc gtagttcttt ttgctggatt
     6781 aatcgggcat tatgtatatc aaggaaacta cgaacagatt tatattactt ttacattcag
     6841 ttttgtgttc atctgctttg aaatggtcgc aggatttttc cagttgctag cttcgctgat
     6901 tgtagatgat cgacgaagaa aattgaaata ccttcttttc gcaccactat atatgctttt
     6961 gttctggata gtgaacgcga taacgatcgt gacgactttc attccagcag tcaaaacgat
     7021 tttaggttat ggaagcggga catggaaaag cccggaacgt acaaaaaaat aaaagatgca
     7081 ccgtttttta gacaaacagc ctg
//
LOCUS       DASJST010000138         6955 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJST010000138 DASJST010000000
VERSION     DASJST010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.140"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..1139)
                     /locus_tag="U4B05_002402"
     CDS             complement(36..1139)
                     /locus_tag="U4B05_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2051516.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1361..1831)
                     /locus_tag="U4B05_002403"
     CDS             complement(1361..1831)
                     /locus_tag="U4B05_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN2051517.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2164..3804
                     /locus_tag="U4B05_002404"
     CDS             2164..3804
                     /locus_tag="U4B05_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN2051518.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3958..5280
                     /locus_tag="U4B05_002405"
     CDS             3958..5280
                     /locus_tag="U4B05_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN2051519.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5342..5773)
                     /locus_tag="U4B05_002406"
     CDS             complement(5342..5773)
                     /locus_tag="U4B05_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2051520.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5837..6673)
                     /locus_tag="U4B05_002407"
     CDS             complement(5837..6673)
                     /locus_tag="U4B05_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN2051521.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         2064 a         1444 c         1256 g         2191 t
ORIGIN      
        1 tctgccgcac tcctattttt ttcgtcattt gtttcttaaa taaaacgatt ctcacgcaat
       61 tttatttttt tgatatcttc tactgtttgt gttccagcta attgcatgac catttccaat
      121 tctgttttaa agaaatcaaa cacttgatgg actcctgtac ttcctcctaa agacaatcca
      181 taaataacgg gtctgccaat agcaacaaga tctgcacctg atgcgattgc tttgaaaaca
      241 tgctgtccgc gtcgtacacc gctgtcaaac acgattggta cacgtttatc tactgcttct
      301 gccacatatt gcaacgaatc aaacgctgct ggaccaccat ctagttgacg gccaccatga
      361 ttagataccc agattccgcc agcaccagat tctaatgaac gatagacatc ttcttctgac
      421 tggacacctt tcacatagac tggcaaatca gaatgagcag caataaattc tacatctttt
      481 ggactaagct tctgtttgga agatttgtac acagcatcca tcgtttggcc tactcctgat
      541 tgataagcct gaacgatcgg cattggcagt gggaaggtaa acccatttct gcggtctgtt
      601 tcacggtttc cgccaactgt tgcatccgct gtcaatacga ttgcttttgc tccattacgc
      661 ttggccactt caagaatatc caaattgatc ccatcatctt tgctcatata aaattgaaac
      721 cattgcggtg ctttctcccc gcctgcttct cttatctctt ctaatgtaca agaagcgtaa
      781 gaacttgctg tatagatcgt cccaaaatct gctacacctt ttgcagaagc tttctccgcc
      841 ttcacatgag ctaagccatg agcagcaact ggtgccatga tgattggtgc agtcagcgtt
      901 tctttgtcaa aatgtgtagt tgtatcgggt aattctacat cttttaatac atgaggaata
      961 atcagctgat gattgaatgc ccgttcattt tcttgataag taaataagtc gccagcacca
     1021 ctgctgatgt aaccataacc accagctgga atcacttgtg aagcggctat ttctaaatct
     1081 tccatattga taaaatccac tattccttca tttgtacctg cttgatacgt tttttccatt
     1141 tcaatgcctt ccctctataa aaaattgaat gaataatatg atagcgttta cacactttat
     1201 attactcttc cgtattgata caggcaaata atctgtttac atttcagtca ttccagttgt
     1261 tactactctg aaagaaatgt ttagaacaca aatagataaa aaacgatggg actttgacaa
     1321 aagtcgtgtc aaagtccctt ttacgaaata ctgtggatgc ctattgaaat aagtgcgggt
     1381 tgtttctttg tatttcaatc gtttcttgcc attcttcgtt taattttgga ttaccgtttt
     1441 cttccttgaa cgcataaggt cccaatccaa aaagctcagt tagtatttga ttcacgattc
     1501 catatagctc ggtgtgatcg gacacatgat tattgaagat catctcatta tcaccacgtt
     1561 tgaatctcac attcgggctc tcatgatgaa cctctatgca gttttcccct ttttttaact
     1621 gacggatttc tgaaaacatc tcataaatat cttctccaat aaaaccttca gcaagcgcca
     1681 gactgtccac aagaccgcct actgtcaact cagagattat cgctttgttg gcaccaagct
     1741 ttaatgtata gcttgttgct tctactacgc cgcatgcatt gcaaccacct tctgatagtt
     1801 cgctttttct gatcaattga acttctttca ttttctgttc ctcctcttag ttttctactt
     1861 atgtattttt tatcacctga cgtaagtgta ttttatcgtc acgatcacgt gatttattct
     1921 tcagagattc tatttatcta tctaacatat cctttattct tctacttttc tttctttagc
     1981 atataagaga tacgcatagt aatcaagcaa ggattgttga tataaaaaga acaaataccg
     2041 ctattttgat ttcttttgac tctattactc atataaataa aatttattat acctgctctt
     2101 tttgactata tcctcatctg actcctatac ttcgtttgta ttaaaactta ggaggaatca
     2161 ttcatgctta cagggctaaa cttattaaat aatccttttc taaataaagg aacagctttt
     2221 acaaaagaag aacgggcaaa atacggaatc actggtatgc ttccaagtac tgtccaaaca
     2281 ttggaacaac aatctgtcca agcttacggt caatacttaa gcaaacagac cgatttggaa
     2341 aaacgtatct tcttaatgaa tctattcaat acgaaccgga cattgtttta taaattgatg
     2401 ggcgaacatt tagttgaatt catgcctgtc gtttatgatc ctgtcgttgc ggattcaatc
     2461 gaacaataca acgaaatttt cttgaaacca caagatgctg catttctttc aatcgacgaa
     2521 ccagaaaata tcaaagcaag tttgaaaaac gcagcagatg gacgcgacat tcgtttgatc
     2581 gttgtgacag atgcagaagg aatcttaggt atgggtgact ggggtgtgaa cggtgttgac
     2641 atcgctatcg gtaaattgat ggtctacact gctgcggctg gtatcaatcc tgcacaagtc
     2701 ttgcctgtat caatcgacgc tggtactaat aacgaagaat tattgaataa tccattatac
     2761 ttagggaatc gtcatgctcg tgtagaaggc gaaacttact acgaattcat tgatcaattc
     2821 gttcaatcag ctactgaatt attcccagaa ttgttattac attgggaaga ttttggacga
     2881 ggaaatgctg caacgatctt agaaaaatac gaagataaaa tcactacttt caatgatgat
     2941 atccaaggca caggaatcgt tgtactagct ggtgtattag gtggattgaa tatatcaggt
     3001 gagcaattga aagatcaaac gatcttgaca tttggtgctg gtactgctgg tgtaggtatc
     3061 gcaaacatcc ttttagatga aatgatccgt caaggtgtac ctgaagaaga agcacgccaa
     3121 catttctatc aagtagataa acagggttta ttgtttgaag atacaaaagg attgactcct
     3181 ggtcaaatcc cattcgctcg taaacgctca gaattcacta atgctgatga attgacaaac
     3241 ttagaagcag ttgtaaaagc aatccatcca acgatcatga tcggtacttc tacccaacct
     3301 ggagcattca ctgaagaaat cgtgaaagaa atggcggctc ataccccacg tccaatcatc
     3361 atgcctttgt ctaatccaac aaaattggca gaagcaaaag caaaagattt gattgaatgg
     3421 acggacggaa aagctttagt tggaacaggt atccctgctg ctgatgtgga atataacggt
     3481 gtaacttacc aaatcggtca agcaaataat gcgttgatgt acccaggttt agggcttggt
     3541 ttgattgctt caacagctac acgagtaaat gctgaaatca tttctcaagc tagtcgagca
     3601 ttaggcggaa tcgttgatgt aacaaaacca ggagcagcta tcttgcctcc agtagcaaaa
     3661 atcactgaat tctctcaaac gattgctgaa actgtcgcaa aatcagttgt tgcacaaaaa
     3721 ttgaatcgtg aagaaatcac ggatatcaaa gaagctgttg aatctgctaa atgggttcct
     3781 gaatataaat ctttagaaga ttaagattgt tatagtaaaa gaacatacag aaactttgta
     3841 tgacgtctaa aaagaaaaaa gcgactgcgg caaaacagca gccagccaac tactgctctt
     3901 ttgccgcttc tctttggctt ttttcatacg atacagaata agaaaggatc tgtaaaaatg
     3961 gcacaaaaaa caacaaccgc aactgcagtt gaggaaaaga aaaaagaagg gttttggtca
     4021 acaaaaatta ccggcgtaag tttgcccctt tatttgatca tggtccttgt cctgatcatt
     4081 gcaatggctt taggaaaatt accgacgaat atgattggcc caatcgctgt tttggtcatt
     4141 ttcggtaatt tatttcattt tatcggaaca aaaatcccta tcgtcaaaag ttatttaggt
     4201 ggtggctccg tttttgcgat ctttgcctct gccgcgattg ctacatttgg gatcttacct
     4261 gagtatgtag taaaatccac agaaaacttt gtcagcaata tgggcttttt agacttttat
     4321 attgctgcct tgattactgg tagcatcctt ggtatgaacc gtaatctatt gatgaaagct
     4381 tctgttcgtt ttattcccgt tgcacttatt tcaatggtcg tctccttttt tgccgtagga
     4441 ctcgtaggta tgcttatcgg aaatggtttt gcaaattccg ttttatatat ctcattgccc
     4501 gcaatggctg gtggtgttgg agccggagct gtacctttat caacgattta tgcaaatgtt
     4561 ttaggtacgg acgcttcttc tatcatttcc cgcctgattc ctgcgacagc aatgacaaat
     4621 gtcctagcta tcattggtgc agcgttagtt gctcgtgcgg gacaatcaat gccaaaattc
     4681 aacggtaatg gtaaattaat ggaaaaaggc gaccttggcg atggaaaacc tagaaatgta
     4741 aaaccttcca ttccacaatt aggtgtcggt ttgatggtat cattgacttt cttcatctta
     4801 ggacaaatct gtaactattt tgttcctaaa gtccatgctt acgcattcat gatcattatc
     4861 gttgttatct gtaaaatcgc aaacctatta ccagaatatt acgaagatgc agctatcatg
     4921 ttcaacaact tgattgtcaa aaacttgaca gcagctgttc ttgccggtat cggtattgcg
     4981 ttattgaatc taaatgtatt ggcttctgct ttgacatggc aatttgttgt cttatgtttg
     5041 acaagtgtta tcgcgatttc agtagtttca ggatttgtcg gtcgtttgtt tggtttatat
     5101 ccaatcgaat caactattac tgctggctta tgcaacaact cgatgggtgg tacaggaaat
     5161 gtagctgtct tatctgcagc taatcggatg gaacttattg cttttgctca aatgggcaat
     5221 cggcttggtg gtgccatcgt tttgatcatc tcaggtttct tgatgcaatt actttcttag
     5281 tcaaaaaaaa tacctgatca tcaagtatcg gcttgatgac caggtatttt ttgataaaaa
     5341 tttacaggat tttcaaaaat cttttttgca ttttatcgga atgataaccg atgcgttcat
     5401 aaaatcggtg tgcttctact ctgtattctg cagaattaag tcttattgct gatattcctc
     5461 gtcttttcgc ttcttctacg ccagtcatca aggcctttcc gattcctctt ttttcagcat
     5521 gtttagacac agcaagtgcc ataatattga acatcggatc gctgaaaatc gattcatagg
     5581 actccgcttg tacatagcca gctgcttttt tagaagtagg atcttcatag accaagaaaa
     5641 aatttttttc tggctccttc aacagtttct tcatttgtct caccgtaacg gttaatggta
     5701 catcatatcc taattgttcc gcattgattt ctcgtagtgc ttctgcatct cctaaacgca
     5761 tttcccgcaa catccaaatc accagccaat ttttattttt ttaaatgaat ttttttatca
     5821 cagtatactc cttttttcat ttagaacaat atgtttcagt gattttgact tgcgttagtc
     5881 tagattctcc taatgtttgt aagatgacaa aggcattgta acaattgacc aaaagaacca
     5941 cgaggacaat tacttgaaaa ataaaggtca ctttttttgg agacaaaacc ctgctaaatc
     6001 ttgctcccca caaacctcca ataacagcag cggggataac ataaaacaac atagataaat
     6061 caaagcgcat aaagctactt gttaaagcaa tcgttaccaa ttttgccagt tgagaaaaga
     6121 aaatcgtaga tagagaatat agtgttgctt cttttattgg caatgcaaac atcagcatca
     6181 acaaagaaac attgactggt ccaccaccaa tacctaaaaa actagctaaa aagcccagaa
     6241 cccccccaca tataaggtac caagtccatg acgtcaaatg aaattttggc tgatgatgct
     6301 tggtgtagaa gaacgcaaaa accaaggtca ataccgtcaa aaaaatttgg atcagctgca
     6361 catgcttctc gttttcaaat aactgtaaaa aaacttcgaa tacaatgttg ccgactactc
     6421 ctccaacaac agctccacta gatacccaac cgacaatctg ccaattcagt gatttcccgc
     6481 ttgatacttg tcgcacagtc gaaacaatgg acatcgtgaa taccgcaact gttgagtaaa
     6541 aagaaatccc tgcaacagaa tgggcgccga taagatcaag aatcggtttg atcaacacgc
     6601 ctccacccat cccggaaata gcgccaactg tgttcgccag aacgatcacc acaaaataaa
     6661 tcagaccaat catcttgtat ttcctttctc ttgtttatat cttttagcga ttgttactct
     6721 tttcttttat tatacagtaa aagaaacaaa cgaggcttcg aaagcttttc tactttttaa
     6781 ggaaatttag tgctaaacga aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
     6841 aacagccaaa caaaaatagc tcctcataat tttcgtttgg ttgaacttat taccgcagat
     6901 gcaagctttt gaacaccgtt taagaaagag gtcgtgagcc tgccgttcag ctccg
//
LOCUS       DASJST010000139         6873 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJST010000139 DASJST010000000
VERSION     DASJST010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.141"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(75..950)
                     /locus_tag="U4B05_002408"
     CDS             complement(75..950)
                     /locus_tag="U4B05_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2051522.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1059..2828)
                     /gene="aspS"
                     /locus_tag="U4B05_002409"
     CDS             complement(1059..2828)
                     /gene="aspS"
                     /locus_tag="U4B05_002409"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN2051523.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2843..4135)
                     /locus_tag="U4B05_002410"
     CDS             complement(2843..4135)
                     /locus_tag="U4B05_002410"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN2051524.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4529..5476
                     /locus_tag="U4B05_002411"
     CDS             4529..5476
                     /locus_tag="U4B05_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2051525.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5565..6824)
                     /locus_tag="U4B05_002412"
     CDS             complement(5565..6824)
                     /locus_tag="U4B05_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN2051526.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
BASE COUNT         1968 a         1474 c         1233 g         2198 t
ORIGIN      
        1 tatggtagaa aaaaactgca gacaaacgaa cgtctgtcta cagcagtatt tcatgaattt
       61 ttgtcaaact cgatctaaat gccatcctca tagaaacgac caagtatctg gacgttttct
      121 gcaatactta caaaagcttg tggatcagaa gctttcattg cttctctaag tagaggcagt
      181 tcgtatcgtg taacgatcgt catcaatact gtctgcttat catgcttata agcaccttct
      241 gcttcattga tgatcgtgat ccctctacgc atacagtttt ggatttcatt tactactttt
      301 tctggatgtt tcgttacgat cattacctgc atcttctttt gtttagtata aacgacatca
      361 gttacttttc cactgacgaa aatagacaac gtactgtaaa acatatattg ccagccaaat
      421 agaattccgg caacaaaaac gatcaatgca ttgaagtaga tcgaaataga accaatcgac
      481 tttcctgttt ttttacgaat cgtgatactc acgatatcta gaccgcccga tgataggccg
      541 tttctcaacg ctagcccaat acctagtccc atcacagccc ccccaaacaa cgcacagatg
      601 attggatcgg tagataggac tgtttccggt aaaatatgga tacaaaggga ggtcaaagtg
      661 actgtgataa aagtgaaaat tgtaaacttc ttcccgattt ttaaccacgc caatacaaac
      721 aaaggtatat tcagtaaata caaagttgtt gataccggta cttcgaatcc tatcagtgat
      781 tttgacaaag tcgtcaaaat ctgagctaga ccagtaatac cacttgaata aatatgtcct
      841 ggctgataaa agaaattcaa tgcgactgat gcgatcaacg cataaacgat agataccgaa
      901 agcttggtcg tgtaatcatg ataagcccaa ttttcgaatg tttttttcat ggaaaagccc
      961 cgctttatat gttatggctt cattataaag tgtttttcaa ttaaatcaag gttgtcaaaa
     1021 gaagcgtatt cgacaaatcc tttttacctt tttatcggct attcttctat attcgttaca
     1081 tcaatattca attcgaacaa ttgtaaaggc gaaaccatac ttggtgcatc actcatcaca
     1141 tctgctgctt ttccgttttt aggaaaggca atgacttcac gaatattatc ttctccggct
     1201 aataacattg caaaacggtc caatcctaat gcaataccgc catgtggagg gaatccgtaa
     1261 tctaaagctg ttaacaagaa accaaattgt tcttccattt gttcttttgt aaagcctaga
     1321 gtttctagta ctttttcttg aagctcacga gtatgaatac ggatagaacc tccgcctagt
     1381 tcataaccgt tcaagacgat atcataagct tctgcataga cttttgcagg gtcttcagcc
     1441 aataacggga tatcttcttc ttttggcatc gtgaatggat ggtgagcaga gacatatcga
     1501 ccttcttctt ctgaaaattc aaattgtggc caatcagtta cccataagaa attgaatttc
     1561 gattcatcaa ttagtcctaa ttcttttcct aaacgtgtcc ggatagcacc taatgcagca
     1621 gcgacgattt cacttttatc tgcgccaaac atcagcaagt cacctggttt tgcatctgtt
     1681 gctttaatga ttgcttctgt tgcttctccc atgaatttag caattggacc tttcaatccg
     1741 tcttcttcta ctttcagcca tgcaagcccc ttagctccga attgtcctac atattgacct
     1801 aactgatcca tgtcttttcg tgagtagcga tctgcagcgc ctttagcatt caatgctttc
     1861 acgacaccgc cgttttcaag agccatttgg aagactttga attctacttc tttgactgta
     1921 tcgcttagat cgatcaattc catatcaaaa cgtgtatccg gtttgtcgct gccgtagcga
     1981 gccattgctt catcataggt catacgagga aatggcaatg tgacttcgat accgcgaact
     2041 tctttcatta ccttcgcaat caatccctct gtgtaagttt ggatttcttc tgctgttaag
     2101 aacgttgttt caatatctac ttgcgtaaat tcaggctgac ggtctccacg taaatcttca
     2161 tcgcggaaac aacgaacgat ttgataataa cggtcgaatc cagcattcat cagcaattgt
     2221 ttaaacaatt gaggagattg cggcaacgca tagaaatgtc cagcatggac acgtgaagga
     2281 actaggtagt ctcttgcgcc ttctggtgta gatttcccta ggtaaggtgt ttcgatatcg
     2341 agaaagtcat tatcatctaa ataatgacga atcgcttttg ttgtcgcatt tcttaatttg
     2401 atgtttttag tcatttttgg acgacgcaga tccaaataac gatatttcaa acgtatttcg
     2461 tcactgattg cttgatcatc ttcaatttga aatggcgggg tttttgcttg attcaaaatc
     2521 acgatttcac ttgcaatcac ttcgaattca ccagtggcca ttttaggatt tactgcattt
     2581 tctccgcgtc ttctaacttc cccagtcact tcgatcacgt attcactgcg acatttatcc
     2641 gcgatttccc atgcttcttt gttgatttca ggattaaaga cgatttgaac gattccttca
     2701 cgatcacgta ggtcgataaa gattactccc ccaaggtctc gtcttttttg tacccatcct
     2761 tttaatgtaa ctgtttgttc taattgttgt tcggaaacta atccgcaata gatcgttctt
     2821 tgtgccattt atttttttcc tcctactcga ataaagtcat ttcatcatag acttcatcaa
     2881 aatgttcata tatctcttct aatggaaaac ttttttcttt tcggcttgcc attgctttga
     2941 cattaatcgt ttgactagca agctcatctt caccgatcac caatactaat ttggcattcg
     3001 ctttatctgc tgtcttaaat tgcgcttttg ctttacggtt catgaagtcg cggtcagctg
     3061 aaaaaccaaa gtttcgaatc gcttggacca ctttcaatgc ttcgatattt gtttcgtctc
     3121 cgagtgcgac cacgtaagca tccaactcat taaatgcagg gataactact tcctctgcct
     3181 ccatagtcaa gagtaatcgc tcgataccca ttgcaaatcc gaagccaggt gttgaaggac
     3241 cacctagttc ttccactaga ttgtcataac gtccgcctgc gcaaatcgtt gcttgtgccc
     3301 ccatttttgg tgcgtcactc atgatttcaa aaatcgtatg tgtgtaataa tctagtccgc
     3361 gaaccatatt actgtcgatt tcataaggaa cagccaatgc atccagcatg gaaacaacgg
     3421 tatcaaaatg tttttgagca ggctcgctta gataatctaa gatagatgga gcttctgcca
     3481 caaatttttg atctcgtttg tctttactgt ccaatacgcg taatggattt tcatgaaggc
     3541 ggcgtttaga gtcttcactc agctctgatt caaaaggtaa aagatagtcg atcaatgctt
     3601 gacgataggc ttgacgcgtt tctttatcac ctaatgaatt gatgactagc cggatttgcg
     3661 tgatccccaa ctgtttgaag aaatcaagtg ccatcaccat gctctccaca tcagttgcag
     3721 ggttttctga tccaaatgct tctactccga tttgatggaa ttgacgtaac cgtccttttt
     3781 gagggcgttc gtagcgaaac attggtccca tataaaaagt tttgaacggt ttcgcatact
     3841 ctgggccgaa caatttatgc tcaacaaaag aacgaacgat tggtgctgta ccttctggtc
     3901 gaagcgtcac atggcgttcc cctttgtcat aaaaatcata catctctttt gaaacgatat
     3961 ccgttgtatc ccctacactt cgggatatca cttcatagtg ttcaaaaatc ggtgtacgca
     4021 tttcattata ctgataatct ctgaataata aacgtgctgt ttcttcgaca aactgccatt
     4081 tttctgattc gcctggcaaa atatcatttg ttccttttgg tctttggaaa ctcatagttt
     4141 tctctccctt tcttagctat gtaaataaaa aaatcaccct tgttcatcat gaacaagggt
     4201 gaatgaaatt catacacggt accacctaat tttgaaagat ttctctttcc tcttacgaat
     4261 aacgctcgta cgcgggacgg cttttcactc gtccatctcc agagtgtctt taggctaatg
     4321 aaactttaga tcattttcag cctcgatgat cctctcttgt aaagtttcaa aaacttactc
     4381 gctctttcat tgacattcga tattacttat aacctactaa aaaccagtct caaagtcaag
     4441 agatatctga ctatttcccc tcacaaaaac gttttagttc agctttttgg tattttgttt
     4501 tagactagac aaaaaggagg aatcagctat ggcagaaata agaatcggac attcacaagt
     4561 ctacgcagaa cagcttggat taggagcaaa tgccgttgga ggacataatc tttttgacgg
     4621 tttggaagat gagacaggaa aacaagtagt tcgtacagct ttgaatagcg ggatcaattt
     4681 gatcgatacc gcttatgctt acggaaacgg ccgttccgaa gaactgattg gcgaggtact
     4741 gaaggaaaaa gaatatgatc gctcacgtgt cgtcatcgct acaaaagctg ctcatgtacc
     4801 aaataaaggg cgaacatttg ataattctcc tgagtttctc aaacaatcgg tggaagatgc
     4861 actaaaacgt ctgcaaactg attacatcga cattttttat atccactttc cggatgaaag
     4921 cacaccaaaa aatgaatcag ttgctacatt gcacgaatta aaagaagcag gaaaaatccg
     4981 agcagtcggt gtgtcaaact tcacgttaga acaattgaaa gaagcgaatg cagatggata
     5041 tgtcgatgta gtagaagata aatacagttt gatccatcgg caggctgaaa aagaattatt
     5101 cccttatctt gaaaaaaaca aaatcagttt tgttccctat tttcctcttg cttccggttt
     5161 gctgacagga aaatatgaac ttggtgagga aaaacaattt ggtgaaggag atcctcgaaa
     5221 aagaaatcca gatttccaag gagaacgttt cagagaaatt ttaaccgctg tcgatgtact
     5281 tcgccccatt gctaaaagat accaagccac tcctgcgcaa cttgttttag cttggtacat
     5341 gaagaatcca cgagtgtctg tagttatacc tggagctaag cgtccagagc aagtatctga
     5401 caatgtccaa gctttagatt tgcatttatc caatgaagat tatcaaacca ttgatgaagc
     5461 atttcgtgga ttttgatcaa ttgaccttat caatttatag taatatgaaa aaaggaagat
     5521 cagcactgtt tgatcttcct ttttttcgat aagtttatgc taatctaatt cttgtaaagt
     5581 agcgctcggc tattcggata agtcggaaac tgattgaatg gaatcgtcca atcttgttca
     5641 ggtacagaga actcaaactt atttactagc tgatcggaaa acacacgtag actatgaaga
     5701 gtgatccatt ctcctgcaca gcgatgcatt tgtcgaaaat ttcccccgcc ttgtgcaatc
     5761 atctcatatt cttctttata ggaaatatct tttgctttcc ctacataccg tttgatcata
     5821 aaggaatctg gcgcttcaac tgttcgttca tcatggtctg ttccatataa atctaaaata
     5881 acccaactgc cttctggtat acgataacca tctacttcta cttctttcag cgaaatcgca
     5941 ggtagcattg ggaagaaagg ataatagcgt ctcatttctt gaataaacgg atcttgtaac
     6001 gtcgaaaagt cattttttaa ttgctgatac aaatctggtc tactgaaaag tgcatgtccc
     6061 atcaatgccg cccacacagt tagagcaacc gttggacgga tgatgttcaa taattcaaca
     6121 gctgccactt ccagaggtaa aagctgacca tctaaatctg tggcattggc aaaagcatat
     6181 aatgccacat tttcttttcc agccacagga ttttgtcgag cttcttcaat caagccttgc
     6241 gcccattttt ctgatttttt gcgattttcg acacctttta tgtgatcaat cggagaagta
     6301 aacgttccgc tgatcataga gatttgatat tctgctaatt tttcgacctc atctttagaa
     6361 atcgctgata aattgatacc tgcccactcg caaatagatg taaagaaaac catcttactt
     6421 aaatcaaata gttcaaattg accatgctgt gcttcaagtg cttgtgtcag attcttatct
     6481 aagatacgat gataatcttc cattctttcc ggtgtcatta gatccataaa aatatttttc
     6541 cgatggtgat gagcagctcc atccaaagtc tgtactccac cttgaccaaa aagtgtcttc
     6601 aatactaatt ttggcatagc accttttcgt ttgaaattcc ttggatcata aaatactttt
     6661 gctgctgatg atccgtagat cgtgatagct tctttattga aaatctttgc tttgacgacg
     6721 ggagcgtccg cttcatggcg taactcctcc aacatgttgt atcctttttg atacaatttt
     6781 tttaaatctg tgattttgat atctactact ggtacttctt tcatcttcag caacctccta
     6841 taaaaaataa cttgtataat ttaagcttaa ctt
//
LOCUS       DASJST010000140         6712 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJST010000140 DASJST010000000
VERSION     DASJST010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.142"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..615
                     /locus_tag="U4B05_002413"
     CDS             <1..615
                     /locus_tag="U4B05_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2051527.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="U4B05_002414"
     CDS             612..1385
                     /locus_tag="U4B05_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2051528.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="U4B05_002415"
     CDS             1382..1780
                     /locus_tag="U4B05_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN2051529.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="U4B05_002416"
     CDS             1965..2636
                     /locus_tag="U4B05_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051530.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="U4B05_002417"
     CDS             2695..3135
                     /locus_tag="U4B05_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051531.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="U4B05_002418"
     CDS             3147..3815
                     /locus_tag="U4B05_002418"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2051532.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="U4B05_002419"
     CDS             3879..4502
                     /locus_tag="U4B05_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN2051533.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="U4B05_002420"
     CDS             4558..4944
                     /locus_tag="U4B05_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN2051534.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="U4B05_002421"
     CDS             5157..6548
                     /locus_tag="U4B05_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN2051535.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2139 a         1087 c         1493 g         1993 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa ta
//
LOCUS       DASJST010000141         6681 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJST010000141 DASJST010000000
VERSION     DASJST010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.143"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(7..465)
                     /locus_tag="U4B05_002422"
     CDS             complement(7..465)
                     /locus_tag="U4B05_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2051536.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(478..1809)
                     /locus_tag="U4B05_002423"
     CDS             complement(478..1809)
                     /locus_tag="U4B05_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN2051537.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1820..3262)
                     /locus_tag="U4B05_002424"
     CDS             complement(1820..3262)
                     /locus_tag="U4B05_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2051538.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3249..4013)
                     /locus_tag="U4B05_002425"
     CDS             complement(3249..4013)
                     /locus_tag="U4B05_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN2051539.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4117..4992)
                     /locus_tag="U4B05_002426"
     CDS             complement(4117..4992)
                     /locus_tag="U4B05_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2051540.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5247..5331)
                     /locus_tag="U4B05_002427"
     tRNA            complement(5247..5331)
                     /locus_tag="U4B05_002427"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5295..5297),aa:Leu,seq:caa)
     gene            complement(5349..5420)
                     /locus_tag="U4B05_002428"
     tRNA            complement(5349..5420)
                     /locus_tag="U4B05_002428"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5386..5388),aa:Gln,seq:ttg)
     gene            complement(5430..5502)
                     /locus_tag="U4B05_002429"
     tRNA            complement(5430..5502)
                     /locus_tag="U4B05_002429"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5467..5469),aa:His,seq:gtg)
     gene            complement(5564..5648)
                     /locus_tag="U4B05_002430"
     tRNA            complement(5564..5648)
                     /locus_tag="U4B05_002430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5612..5614),aa:Leu,seq:caa)
     gene            complement(5669..5739)
                     /locus_tag="U4B05_002431"
     tRNA            complement(5669..5739)
                     /locus_tag="U4B05_002431"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5705..5707),aa:Cys,seq:gca)
     gene            complement(5785..5856)
                     /locus_tag="U4B05_002432"
     tRNA            complement(5785..5856)
                     /locus_tag="U4B05_002432"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5822..5824),aa:Gln,seq:ttg)
     gene            complement(5868..5940)
                     /locus_tag="U4B05_002433"
     tRNA            complement(5868..5940)
                     /locus_tag="U4B05_002433"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5905..5907),aa:His,seq:gtg)
     gene            complement(5943..6016)
                     /locus_tag="U4B05_002434"
     tRNA            complement(5943..6016)
                     /locus_tag="U4B05_002434"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5982..5984),aa:Trp,seq:cca)
     gene            complement(6023..6103)
                     /locus_tag="U4B05_002435"
     tRNA            complement(6023..6103)
                     /locus_tag="U4B05_002435"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6067..6069),aa:Tyr,seq:gta)
     gene            complement(6105..6180)
                     /locus_tag="U4B05_002436"
     tRNA            complement(6105..6180)
                     /locus_tag="U4B05_002436"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6145..6147),aa:Phe,seq:gaa)
     gene            complement(6216..6288)
                     /locus_tag="U4B05_002437"
     tRNA            complement(6216..6288)
                     /locus_tag="U4B05_002437"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6253..6255),aa:Asp,seq:gtc)
     gene            complement(6298..6370)
                     /locus_tag="U4B05_002438"
     tRNA            complement(6298..6370)
                     /locus_tag="U4B05_002438"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6335..6337),aa:Val,seq:tac)
     gene            complement(6386..6457)
                     /locus_tag="U4B05_002439"
     tRNA            complement(6386..6457)
                     /locus_tag="U4B05_002439"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6422..6424),aa:Glu,seq:ttc)
     gene            complement(6472..6561)
                     /locus_tag="U4B05_002440"
     tRNA            complement(6472..6561)
                     /locus_tag="U4B05_002440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6525..6527),aa:Ser,seq:gga)
     gene            complement(6601..6674)
                     /locus_tag="U4B05_002441"
     tRNA            complement(6601..6674)
                     /locus_tag="U4B05_002441"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6638..6640),aa:Asn,seq:gtt)
BASE COUNT         2004 a         1482 c         1099 g         2096 t
ORIGIN      
        1 cgtacactaa aattcaaaac aactatcttt aatctttact tcttggtttt gttttaataa
       61 atttccttca aaaatgcctg gagaaactac aatgatcacc gtatcttttt gtgcttgctc
      121 caacttcttc aaatctataa tagctaacag tgtcccttct tttacctcct ggttactttc
      181 aacccgtatc tgaaacggac ttccttcgag ttcaactgta tctaaaccca tatggatcag
      241 tatttttctg ccagattcac tttcgatcgt tatggcatgt cgggtaggaa atatattatc
      301 gattattcct ttaactggcg catggatata tccatcatga ttcgttatag cgtatccaac
      361 tcctatcatt tttgaagaaa agacctcgtc ttttactgct gttaatggaa taatttgacc
      421 actagctaca gcttttagaa tgtttcttgt tttaaagaaa tgcacttttt atcacctcta
      481 ctttaatgct ggccaatatt ttttgtttac tttgatcatt tcatctagta ttttttttgc
      541 gactctggca tttggtacgg tttgattcaa tgtaaatgct tgtaaagctt tttgataaga
      601 accttcaaag taagcatcta ctagtaactt ctctactgca acttgtgcct ccatcaatcc
      661 tttatgaaaa tcactgattg gttttcttag tgtaattggc tccacaccag ttgcaccaac
      721 atatgccggt atttcaacaa ctgcatctga ccgtagattc ggaatcgccc cttgattagg
      781 aacgattagc ataaagcgac gacgatcatc attcaacaag gaaatagcta tatcgacgat
      841 atattgtcca tgctccccaa aattaaagtt caatacttct tccacaatac cgcggcgtat
      901 tttatcagcc atttctttgg tatttttctc tcgtccatcc atgaccatat tagcacgtgt
      961 atactctttg tcagtatgct caacgaccat atcaggatat aagtaatact ctaagtaatt
     1021 attcgggata ttacttggaa aatttttcgt gatgactgac atcatttgaa aggttttttg
     1081 ccaagtttta tctcctatat taaaatcggc gacttgcatt tcttttgtta ttagtttttc
     1141 aataatctct ggcataattt ctcgttgcaa ttctttatca taaatcgatg tataccagcc
     1201 aaagtggttt aaaccataat acgtaacgat ccaattctta cgatcataac cgtaattcac
     1261 tgcaatcgtt tcttcaattg aaatagtcat atcacaggca ttaatcattt ttacattcgg
     1321 atatttacga cgaacagctt cagaaacgat cgactctgga tttgtataat tcaaaatcca
     1381 tgctttgggg gcatagtctt gtatatgatc gacaatttcc aataaacctt tgattgagcg
     1441 catcccataa gcaaatcctc cgagtccaca cgtttcttgt cctactaatc catgtttaag
     1501 tggaatcttt tcgtcctttt ctcgcatctc caatcctcca acacgaattt gtgagaaaac
     1561 aaaatcgcat cctgtaaaag caagctttgg atcatccgtt gcacgaatgc gaatatttga
     1621 aaaacctttt cttttcagca taaatttaac aatcaagtac atatccatat ttctttcttc
     1681 atcaatatca tataaacgta tttcagataa tggcaaccgt ttttcattgt tcaaaatggc
     1741 ttgaacgatt cctggtgtat acgtactccc gcctccagct atagtgataa tctgtttttt
     1801 catcatcatc ttttcctttc tattcttcta ataatttatt gaattctgcc ataacataag
     1861 tcacttctaa gccgatgata atttgataat ttttttcagt tggtttaatg atcccgctta
     1921 tccctaattg cttgattcgc tgttcctcga ccatggtggg atccactacc tctacccgta
     1981 ggcgtgtatt acaactatag acatccagga tattggcttt tcctccaaac gcttgtagca
     2041 tttttgttgc catatattca taatttttac ttgctaaccc taactctgtt tcttctaagg
     2101 ttacttcatt gccaaactct gccttagctt cacgtcctaa tgtctgaatg tttctcttat
     2161 tgatcaaata ataaaaagta acgtaataca aggcaaaaaa gaaaattccg attggaagaa
     2221 tcaaaatagc attggtagcg attcgaaaat tgatgagata atcaacaata catgctccga
     2281 atgtaaaacc aattcgtata ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa
     2341 gtgcatggat cacgtacaac ataggagaaa gaaacatgaa actaaattca acaggctcag
     2401 tgatatttgc aataaatgaa gttcctgcac cgcttcccat gattcctttg accatctctt
     2461 tattttcttt ataagctgct ttatagatag ctagacatat agctggtata ccaaacatca
     2521 ttactacaaa aaaaactgat aaaaacagtc cagcctgagg atctccgttg ataaagcgtg
     2581 tcatctctcc tctgaaaact tccttgctag gagatgtata gcttcctaaa tcgtaataaa
     2641 tatacgtatt caacacatgg tgcagtccaa aggggatcag caaacgattc aaaaacataa
     2701 atatccccac acctaatgct cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa
     2761 tttggatgag cggccagata aagcccaaaa aaacagctgc gattgtttgt agaatcatca
     2821 ccatagtcaa tgggaatttt tcgcccgcaa aataagaaaa aactactggt aatttttgtt
     2881 ctttaaagcg attatacgtc catgccgcta ctaaaccgga aataattcca ccaaaaatcc
     2941 ccatattcac ttctgggtcc ataatttcta agcctttttt caaggtagca atcgctaaaa
     3001 aaccagtaag tgcaggtatt cctttgtcct tatttttgac aaaaccgatt gcaactccta
     3061 tagcaaataa gatatccaag tttccaaaaa cggcatttcc agctgcacca attactggga
     3121 tgttcagcat atcgtcagct gataaacgca tgatcaatcc cgctaatggc atcgctgcaa
     3181 ctggaagtag catcgctctt ccaagcttct gcacatttga tttaacattc atttttattc
     3241 ctcctctatt aaatatcctc atatgttact aactcaatca aaggatcatt tttcactttt
     3301 tgagcaaatg ctgaatgaat taaaaattcg acttggtggg tacgaggata cacaaaactt
     3361 gaaccattta acaattcctg atccaaatac gctgcgtgac acatgacttc gatcgtttta
     3421 tcttctctca atatcatgag taaacttgcc aaatattctt ccacactcgt attgatttgt
     3481 cgtttttttt catcagtttc tcctacgaca tcgaaacaag gctcaaaata atcgacatga
     3541 cgaacttcat cttttcgttc aaagttgctc cgaaccggca aatcatattt ttttgctaat
     3601 gtaacaacta cctcctgatt ttcatccaaa gtatgagtat gatgatgact gtctaaatga
     3661 gttggtatga tccccgcatg ataaattttt tgaatctgag cattccattc ctgatacagt
     3721 tgatctttgt caatgttaaa tggatgctga taataagata aggatctgaa ctctccgctt
     3781 tcgataagag tatcgacagt tttgaggagt ggtttgccac acgtcaatgt taaatggact
     3841 cctacaccta gcctaggcat ttcctttctt agtttcacag catgctcgaa acccggcata
     3901 tttgccatca atgtagttga tgtcaaaatt cctcgctgat aagcatccat aatcccataa
     3961 ttcacaccat ggctatagcc aaaatcatcc gaattaaaaa tcacttttcc catttcattc
     4021 tccttctttt ttctactaaa ataggcgcaa ccaaataatc atagtccatt tactatgtgt
     4081 catttggttg cgcctataga tagttacgcc cttattttaa tagattcttg ctagatgaat
     4141 tgccaaatag atgatttcat tttcttgaat ggcaagttga aattgctgtt cgatgatatc
     4201 tttaatcttt agtgctgtac aatacgcttt gctatgttta aatgtttcag taaaaaaacc
     4261 aatattttct actggcgttt cttcctgata aatcaagcgt ttaaccagga atttgatatg
     4321 aaccattaag cgatgataag tcagtgattc tttatcataa acgtttgaat aggatttttc
     4381 tagatgggac aaaatcgtgg tcgtcatttc tactattttt aagctcttta aactattagg
     4441 ctctcctagc tcagcattaa tgatatgcat agtgatgaat cctacttcat ttgaccctaa
     4501 ctcggtttga tatcgatgat tgattcgctc gaccacttct actgctatat catattcatt
     4561 tggataaagg aatctcattt ctggcaacat aattgctgtg acatctctat cttctctttt
     4621 tcgttgaata gcaaaataaa tatgatctgt caatgtaata tagatatttg aatctaatgg
     4681 agaggagatc ctagctgtta tcagttcaat cacttcttcc gcaaaatcca taaattcgat
     4741 tggaatctgc gagactatct cactgaatcg tttgatcttt tctttagaat ctaatacaaa
     4801 taatttgtca gcatcctcaa catcaaatgg cttactcact ttagcagaaa agccaatccc
     4861 tttccccatc gcaatttttt ctattccatc gtcatcgatc aatacagcat tatgattcag
     4921 ctttttcatc acatacaccg ttaattcacc tcctctatat gataaaacaa aacaacttgc
     4981 agactccttc atacatattt ttgtctccaa acaattttta aagacaaaaa acgacctaag
     5041 aaagaaccct ttcttaggtc gtgcctaatt gaatagtagc accctacata atttacaaaa
     5101 atagtttaac acataataaa agcgttatca atatcttttt tactcgtttt atgataatac
     5161 ttgtatcgaa ttgatttgga ggtaattaca taaaaacaaa aaaaatatag caactctcta
     5221 taagaaagtt gctatattga taattatacc ggcggccggg gtcgaaccgg cacgtcctca
     5281 acggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa
     5341 aataataact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag tcccttgcct
     5401 taccgcttgg ctatacccca agaataaagg gcgaatgatg ggaatcgaac ccacgaatgc
     5461 cggagccaca atccggtgcg ttaaccactt cgccacattc gccatcataa aagatgttat
     5521 ttaatttctc aataaaaaac taagatgaag acatcttagt caataccggc ggccggggtc
     5581 gaaccggcac gccctcaacg ggcactggat tttgagtcca gcgcgtctgc caattccgcc
     5641 acgccggcaa gttgatttat tagtactaag gcggtaaccg gatttgaacc ggtgatgaag
     5701 gttttgcaga cctctgcctt accacttggc tataccgcca ttatactatt atcaatctat
     5761 aaaataaaga ataaattata gaaactgggg tagctggatt cgaaccaacg catgagggag
     5821 tcaaagtccc ttgccttacc gcttggctat accccaagaa taaaaagggc gaatgatggg
     5881 aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg ccacattcgc
     5941 catggcaggg gcagcaggaa ttgaacccac actaacggtt ttggagaccg ctgttctacc
     6001 tttaaactat gcccctataa aatggaggag agtggattcg aaccactgaa ccctaaggaa
     6061 cggatttaca gtccgtcgcg tttagccact tcgctactcc tccatggtgg cgcgggacag
     6121 aatcgaactg ccgacacacg gagcttcaat ccgttgctct accaactgag ctaccgcgcc
     6181 atattttaaa attattaatt gaataacaag aacgacggtt ccgacgggaa tcgaacccgc
     6241 gatctcctgc gtgacaggca ggcatgttaa cccctacacc acggaaccag ttcattatgg
     6301 aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct cccagctgag
     6361 ctaaacctcc aacattcaat taaaatgacc cgtacgggat tcgaacccgt gttaccgccg
     6421 tgaaagggcg gtgtcttaac cgcttgacca acgggccagg taaatttgct acggagagta
     6481 agggattcga acccttgaga cagtttggca ccatctacat gatttccaat catgctcctt
     6541 cggcctctcg gacaactctc cagagtgaaa gaagctcaat actctttctc aaaatgagaa
     6601 ctccggcagt aggactcgaa cctacgacat catgattaac agtcatgcgc tactaccaac
     6661 tgagctatgc cggaataaca t
//
LOCUS       DASJST010000142         6668 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJST010000142 DASJST010000000
VERSION     DASJST010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.144"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..894)
                     /locus_tag="U4B05_002442"
     CDS             complement(<1..894)
                     /locus_tag="U4B05_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051541.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYF"
     gene            complement(914..1117)
                     /locus_tag="U4B05_002443"
     CDS             complement(914..1117)
                     /locus_tag="U4B05_002443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051542.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            complement(1118..1807)
                     /locus_tag="U4B05_002444"
     CDS             complement(1118..1807)
                     /locus_tag="U4B05_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEN2051543.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2062..2382)
                     /locus_tag="U4B05_002445"
     CDS             complement(2062..2382)
                     /locus_tag="U4B05_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051544.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(2415..3533)
                     /locus_tag="U4B05_002446"
     CDS             complement(2415..3533)
                     /locus_tag="U4B05_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051545.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(3562..4356)
                     /locus_tag="U4B05_002447"
     CDS             complement(3562..4356)
                     /locus_tag="U4B05_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051546.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(4409..4762)
                     /locus_tag="U4B05_002448"
     CDS             complement(4409..4762)
                     /locus_tag="U4B05_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051547.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            complement(4864..5121)
                     /locus_tag="U4B05_002449"
     CDS             complement(4864..5121)
                     /locus_tag="U4B05_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051548.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            complement(5124..5348)
                     /locus_tag="U4B05_002450"
     CDS             complement(5124..5348)
                     /locus_tag="U4B05_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051549.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(5521..6564)
                     /locus_tag="U4B05_002451"
     CDS             complement(5521..6564)
                     /locus_tag="U4B05_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2051550.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         1840 a         1417 c          840 g         2571 t
ORIGIN      
        1 gaaatacaca atatcattca gctcgctacc cttgatctca aaattattga ctaagtaact
       61 atcaataccc atcaaatctg caaaatctcc atacaaagaa aaatatgatt ttgacaatat
      121 atctatatct gaatcgtcaa acccatttac tattttccca tcattgttag tagttgctaa
      181 cgaagcaata gtgacttttg aattgggttt tacaaatcct atacctaata gttctttcat
      241 ctttttaatt tcaagtttgt tatccttttg ttcggcttta tgctgtttca tattgtaaag
      301 cggataaaat gctgttccgt aagcaccttt tagtacgtta tctataaaat aatcttcaga
      361 acctgttttg aatagaaagt taatcgcagc caaattagat aacagctctt cagtgttgtg
      421 catcaatttt ttatcattta ataaattatc cccaagatat ttcattgcat aataagggtt
      481 tgtttcgcta cttattttta atattttttg atagctattc ctcacgatat taaccaaaat
      541 atctgtctga ttaattgaat tacagtacaa agccactcct aatgccatat aaacattcaa
      601 atcatgtttt gccgtaccgt tgaataaaac gtcttcaact tttgaattat ctcgtctata
      661 ctttttgatc atatttttga tattttgatc attctcttgg tatatttttt tcactcgttc
      721 caatgccttg ggtttgttgg aaacagcaac agcgaagtct agttctttga aatcaatatt
      781 tagtatctgt acctcatcac ctagattata atctctattt acgctcggaa aactgctgaa
      841 atctaactgt tttttaccta tttttggcga aaatattcct tcgatttctg gcaaatgtaa
      901 tcacactctc tccttattta tctttagaaa atttgtcact attctcctta tatttatcaa
      961 taaacctttt taatcgagca atataagcgt taaccctttt aggttcttct tttcgccatt
     1021 catcgtttac gtgaccattt tcaaagaaag aagctaaaag agtttccact tcattaacca
     1081 tatctgcgat cgtatatttt tctgtaccct cactcaatta ttcatgaact gttaagtgtt
     1141 catagctaag ttcattcata attttgttat agcgttttct tgctttttca aattctttct
     1201 gttcgtcaaa ttccacttga ttcttcatgt tgttcttcct ctctgtttga tattttttca
     1261 cttcttcaag tttagcatct agttccgctt taacttctgt cacttcttta agcgcttggg
     1321 ataacttatg agacaatttc gcataagttt ctctcttgta ttcatttcct actcccaact
     1381 cttcggagta gaactcttct agtagtcttg caaaattaac atttctcctg ccagcatagt
     1441 tttttagctt ctcaaaatca gattgaaaga cagcaacact tttgtaatcc ttttcaccct
     1501 caaaatcaaa agcatagtca tactttcctg aaatcaattc atcaataact gtagcaatat
     1561 attgagagat agaaatatta tcttcacttc tatgtcgctc tataagatta tatatatcag
     1621 ggctgacatg aaacgtactt ctcttttttg tttgcctttt tctaactgtt acgaccactt
     1681 ttggaacttc actatctcca ttgatctctt tgtatttttc aataaataat tttctagctt
     1741 gttctcgact ttttgccgga atttcatcta caattttatc ttctaaataa atatagtgtg
     1801 ttctcataca ttcgtcctct atcttcttta ctctcaatct tcatgatctt agttcttacc
     1861 ttgtttactc ttaaaccttt caaaaaaatc tagtcaaaat caaacttgaa atcatcgtct
     1921 atcacaacac ccttaacttc atctttagat ttcccaaagg aacccttatt ttcaaatcct
     1981 ttatttttga atgatgatgg ttatggaaaa cttcctttga aattctgaca agcataacta
     2041 gtcagaactg ttttacttag attaagcttt caaaagatac tgatgaagaa ctaagttctc
     2101 agtaccttcc cattcttcaa taaaaaatat ttctccttct tttacccact ctatagctaa
     2161 tttcttataa tcaaataaac catatgtgtt taagcctttg tctttcatta aactcaaaaa
     2221 ctgatcccct tttaatcttt ctgtatcatc catcagaaat tcaactaaat ctttatccat
     2281 gcgtgcaact ttttcgtctt ttagtgccat tgatttagca tagtcagttg aatcttttag
     2341 aataataaca cctacatttc cttccacaat tttcttcttc atatttacct cctaccatct
     2401 tttccaaaga gtatttagtc aaaatcaaac tcgaaatcat cgtctatcac aacaccctta
     2461 acttcatctt tagatttccc aaaggagccc ttattttcaa atcctttgtt tttaaattct
     2521 aaagcttttt cgacttcttc tttggatatt gtatgaactt ttttcttaac tactccaagt
     2581 ttatacagct cttcaaactc tggtacttcg gatctgaaat acaatccatc agtccgaatt
     2641 tttaatagcc catatgcgct atgaacgtat ttctgttcat atctaaccat acgatctcta
     2701 aatacgcctg ctaccatgga ggaatacgct cgattataca tactgtattt atcgaaggtg
     2761 cttgtttctc cttctcttaa tgatttttgt ccgatagcac caaatacttt cgttctaaga
     2821 tttttataaa gcttcaacgt atcaggatca gtttttaatg gctcgattct ttcaaaatat
     2881 tctttgagta atcctttttt cgtcaaaaat gtgtggctat ctaggacttc catctcttta
     2941 atatcataat aatcaaggat aagtgttagc tcaatctccg ataaccaaat aggctcatta
     3001 gctgttttgg gattttcgcc ataatccgca aacatcgact gtgattttgg attagcatat
     3061 gttccttctt tggctgttcc ttcaaaatta accaaaaaga acatatacgt tcttaaattt
     3121 tcatacggtc ttacttttgg tctatttatc gctctcaaaa tagcttgatc tgtattatat
     3181 tttgctttca aataagtgtt tgacggcaaa ggataatttc tcatgatcct cgaataggct
     3241 tcgttgaaat catattcgat catctttgct ccttcctcaa acaaatccaa gttatacatg
     3301 ctaccggagg tactggctct ataggctgaa cttggaacaa agaaaggtgc atcttccggc
     3361 ttatcatagc ccaaaaaatg tattcctgtt tgcttgttct gacggtaaat gtccatcaaa
     3421 agatatttga tcgtactaaa agaatctaat tttcctcttg tcatccaccc taatccgaca
     3481 agtcttttaa ttttattctg catttcaggt gtgtatatgc tattgtttcc catttatctg
     3541 ctcatctccg gcttttttat tctagaccct atatttgttc attacaccac ttatctcgtc
     3601 tacaaaggct cccagatcgt gtgtaaaggc atcgtagtaa tcttcaatct cactcgattc
     3661 agttctccgg tttcttccgt ttttatttct gaatagatac atctgcgttt ctcttaaatc
     3721 ccttgctttt ttcgcaagtg ctaataaatc cgtaaaatta tcaataacca taatgtcatt
     3781 gatcataaaa ccttcttcat gaccatactt ttcagccatc tcgataaaat cattaacatc
     3841 gaacaactcc aataattctg ctttatattt ctctaacaat gtatttaaat agcgatttct
     3901 caattcccat gaactttcac cagttccgta ttcaaccttg atccctaaat tcttagtgta
     3961 gccttttgct gtgtatcgtc caatgaagtc tcccttattc cgattgctat cttcttcctt
     4021 gaaaaactca cgccaaattc cctttttctt tttaggaatc agacctctgc tcttgaaatc
     4081 attatatttt tttgaagctt cccttcgtgt atctaagtac ctttgcttca gctctgtagc
     4141 tgattcattc gttccatatt caacagtgat ccttaatcgt ttgctatagc cttttgctgt
     4201 atatcgtcca acaaagtcac cgccacttcg atcactactg acttccttaa caaattctct
     4261 ccatatacct tttttgtctt tcggtattaa tcctcgtctt ttcatatctt gatatttttc
     4321 agaagcttct tttagagtat tcttttttgc taccatgtca tcacctcgca atttagttaa
     4381 ctttatacac gtctacaaaa aaatatgatt acttactttt taaattgaaa aactcgttga
     4441 aatgctcttc tttcaacaca acaaaagcat gataacttaa ccttaaaata taatctccat
     4501 attcaactgg ataatctttg gttataactc taattaccgc tcttccgcta tttacaagtt
     4561 cacttttttt cttccccctt cgtactcttc gtatactatg aggttttaca aattcgacta
     4621 cttgcttcca attatcattt ttatattgaa tacacagtgg tgattttttc atttctatat
     4681 acatgttctt ataatcttct actttcttat tttctttaac tagatgttta ttattctcat
     4741 aagacaaatt ttcgcttatc atttaatatt tcccttcctt tttttgcatt atatttagca
     4801 aacattcacc atttgaattt gacatagctt taagacagat attttatctt ttcaaacatt
     4861 aagttatttg tctttttcag aagtggtata ggatcgaagt ccttcacgtt tcattatgtc
     4921 ttctgctttt ccaatgctga tccctttcca atcacgccat cctacgcttc ccttattttg
     4981 acgataggct ttaatagctg attcggatat ttgccatctt gtagccagct cttttgcggt
     5041 gtacttattt gagaaaagta acttctctat cagtgaaata tcaatcattt tattctcact
     5101 cctgaaatct tcattaatca tctctagcta gctatatctt caatcaattc taatatgtcg
     5161 gataaaccat caacagcttc ttcaagttga tcaacggatt cttctgcttt ttcatatctt
     5221 tctgatccct gtagattttt aggtatattt tctttgtact cttcttcttg aattacttct
     5281 aactcatcct ttagctcgga aagtcgctcg cttatttctt ctaacctttt tcttcttctt
     5341 tggttcatca aaatcacctc ttcgcataat ctctacgatt ttaatatacc acttttgtgt
     5401 actacattca atattttttg ttatagattc gtattacaaa aatttcctct aaaaaaataa
     5461 gagcctattt accatttcag taattaagct cttatttcat ttagttgata attctatcaa
     5521 ttaacgtaca gcaaccgcca ttccagtcac attaaatgct ctctcatacg tgcttgcatt
     5581 gatccatttt gcgccaaata cagggtcaac aacatggtat gcaccatttc tgaagccatc
     5641 cacgagaact acatgtccat tccatactgc acgaccccaa ggcatttgtt tgaactctgg
     5701 cgtagcatag ttataagtaa tccaagttac aaccggatta ccattgcgaa tttcattctt
     5761 gattccattc gttcctgaac cagtgatatt gacggcatta ccgccaacgt tttttgccca
     5821 tttaactact gggtcagcca tcatgccttg atacgttcca ttaacgttat gacgccactc
     5881 tcctgagtaa ccatgataag gattattgtc attagctttt ggcatatagt ttacaaaact
     5941 ggttaagctt tgatttgtgg ctacaccttt gtagtgtaaa ccttctaata aagaagctcc
     6001 ttcgcataac attgtatttc cttgccatac aacaggaacg ttcaacactt tttcagtgct
     6061 tggagcaggt gtaggatttg gtacgctgtt tggcattgca taccagcctg taccttcaat
     6121 cttccaacct tgtttactaa ttacgttctt ttcattttga tctaacgtgt agtgatgcca
     6181 attcacgtat ttgttatata aacgtgttac aggaactcta tttgctccgt tgtaggaata
     6241 gaacgcaaca ccttcgtacg tccatcctgt gtgtgaaatc ttatctcttt ctccggcatc
     6301 aactgtgtaa aaatgttcac cgttataccg attatacaaa cgataaacag gagtaccact
     6361 tgttggagct tgccatgcga caccttcgta atgccatcca taacgtttca gattgtctct
     6421 ttctccaacg cttctagtgt atagatgctc tccgctattg ggattataca tacgatacat
     6481 ggaaatagag ctttctgctg aaactgaagg tgataataat actagcccca ttgtgaatag
     6541 aattcctact aaaaattttt tcatttgatt ttaccccttt tcatttttta ttgtcattat
     6601 tgcatgaaaa ttattcgtaa gattgccaat tacataaacg attgttttaa gatcataatc
     6661 gttgttgt
//
LOCUS       DASJST010000143         6582 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJST010000143 DASJST010000000
VERSION     DASJST010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.145"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(53..1183)
                     /locus_tag="U4B05_002452"
     CDS             complement(53..1183)
                     /locus_tag="U4B05_002452"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN2051551.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1243..1470)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1832..3034)
                     /locus_tag="U4B05_002453"
     CDS             complement(1832..3034)
                     /locus_tag="U4B05_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2051552.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3094..3297)
                     /locus_tag="U4B05_002454"
     CDS             complement(3094..3297)
                     /locus_tag="U4B05_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN2051553.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3439..4671)
                     /locus_tag="U4B05_002455"
                     /pseudo
     CDS             complement(3439..4671)
                     /locus_tag="U4B05_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4834..5646
                     /locus_tag="U4B05_002456"
     CDS             4834..5646
                     /locus_tag="U4B05_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051554.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1995 a         1328 c         1029 g         2230 t
ORIGIN      
        1 tccatgaaaa gccgactttt tgggacgctt ttttttgcca agcctcacat ttttatagcc
       61 aaacttcttt tgcgacttgc tgcaataatt ggattttctt ccattgttct tcttctgtta
      121 aatggtttcc ttcttctgtt gaagcaaacc cacattgagg actgatactt agattttcca
      181 atggtacaaa ttgttgtgct tcttttattc gttcttttat tttctccgta tcttccaaat
      241 ctggaaactt agaagtaact aatcctaaaa caactttact ttctttgtta ctccaatgtc
      301 ttaaaggagt aaaatctcct gctcggtcag aatcgtattc caaaaagaaa ccatcataat
      361 ttgttgaaaa caactcttct gcaatgggtt catatccacc ggaaaacagc caagaagaag
      421 cgtgattccc gcggcagata tgtgttgtga tcgtcaaatc ttctggtttg tcttccagtg
      481 cgttattgat aatctctgta caaatatggg ccatttcttt cggatcaatt cctttttgaa
      541 gaagtgcttc tcttttagtt tgatcagcta gaaacgccca atttgtatca tcgaattgca
      601 ggtaacggca acctttttca taaaaagcat gaatcgctgc tttgtaagta gaggacaagt
      661 cctcataaaa atcagataga tcaggataaa tctccttgat ttttgaagaa ccatcattag
      721 ataggatctc ttgtcgtaaa atcatcgtgg gactagggat cgttgctttt gcaacaaaag
      781 tatcatctgt ttccactact tctttcagaa aagcaaaatc ttttaaaaat gggtgatttg
      841 gattgaaaga taatttatct actacccgaa tattataagc tggtgctgct tttccgtgga
      901 atgtttgatt atatccagtt tctggaacat agccttcgat accatttagt tcttctaaaa
      961 aatcaagatg ccaaaatcca cgccgaaatt caccatctgt aattcctttg atgccgactg
     1021 ctttttggtc ttccactaat ttttttattt cctggtcttc gattgcacga agttcttctt
     1081 ttgaaagtaa gccttcttga actttttttc ttgcttcttt gatcgattgc gggcgtaaga
     1141 aacttccgac atgatctact gtaaatggtc tatttttgct catttcactc actcttttct
     1201 ttgttttttg taaaaaaaaa tccttttact agcaaatact agtaaaagga cgattatact
     1261 cgtggtacca ccttttttca gaaaaaacat tctttctctc agcctgacaa taaaatcagt
     1321 ggtcttggta acgaaagact gttcgttttt gcctaccttt cgacaaaaaa tcctctaagg
     1381 ccattttcga cagtcaactt ctagcttttt acaccaacca aagcctctct tgaaccattt
     1441 tctgtctact ttccttctca tcggatacta ttagaataaa attagaatag catgtttgaa
     1501 agaaaagtca atagggaata agaacaaaca aaaactaatt tgttattctt agtaatttgt
     1561 ttgtggaata caataaagct tttctaatat gataattgtt cagtcgtcct ttcatcttag
     1621 atcacttcct taaaatgatc atacccgttt caaccacata aaaaaggact taattttgaa
     1681 aaatttccca tcaaaactaa gtccttttta ttatagttat gcaaatttgc ttattttctc
     1741 gctatttccg atacatcttg aacaggagca attcgactga aaacactggt gcaaaagcat
     1801 ttatttgtct tatggtagaa ccgtggaaaa ctcagactgg taataaattg tcattttttg
     1861 aaagaatagc gccctcttga acattatcaa tcgcacttac cacttgttga tctgactttg
     1921 catataagtg accgtaagtt cctaaagtga tttgaatatt cgcatggccc aatcgttctt
     1981 gaatagatag cggttgaata ttctgctcaa ttagaaatgc aacatgagaa tgtcgtagcg
     2041 cgtgaagctt aatcggtttg acacccgctt ttttagcagc ttcttttaac cagttcgcaa
     2101 aagaataagg tggcgtaaat gttccgtcca actgagcaac atattcaaaa tttccaatac
     2161 gtttttgcaa gtcataccac tgtctgagtt ttaaggaaag cttcttcccc aacccaatcg
     2221 ttcgataagc acatttagtt ttgggagtcg aaaagtacca atcatctttt gtattataaa
     2281 tcaatgtctt ctcaatcgtt atcgtgtttt tctcaaaatc tacattcgac caagacaaag
     2341 cttcaagttc tccaatccga atgccagtat aaaagagcat ttcatacaca atcttccgat
     2401 ggtgggtttg aatcttatca tccttcaatt cattcatcac tttttgaaac tcactgtgtt
     2461 cccaaaactc aactttctta ttctcagaag gaagcgaaga aacatcttca acgggatttt
     2521 tagcaatcac cccctcttta acagcccgat ccaatacaat ttttaggtgc cctcgaatcc
     2581 gattgatata agattttgat aacgtttcgc cattaacttt tttccgtgaa ttacgtgact
     2641 ctgtctggct tagttcaaat aaccaatcct caatatcgtg acttgtaata tctcgaacac
     2701 gaaaatgata gaaaaactgc atacgctttt cgaaaatgaa ttgcgcattc tcatatgttt
     2761 gacttttgac ctgtctctcg taccatttta caaacctatc cttgtaaaac tcttcgaaag
     2821 tcatttgatt ggtagaacac agttgattag gaattgtctt gttaaaatca tgtgtttcca
     2881 tgtacttgtc ataagcaatt ttggcatctt tttgtgaagc ataacctcgt ttagtcttct
     2941 gaacctttct tccattttca tctataccta gatacgctcg gaagaaccat ttttttgtct
     3001 ttttatcttg gtaaatattt ttaatttttg gcatcgttcc attcctccct gtacttgaga
     3061 aaagctactt taatagtacc gcgcttgtac tgtttattcc gcaacttgaa tgtgaaaaag
     3121 atgttcaatt actcgggatg gcaccacacc aacttggcga ttattataaa aatcaatacc
     3181 ttcaatcctt gcaagatatg ccttgctctc tttaatcatt cgtgcagctt gactaggttt
     3241 aaagcccatc tctatcaaaa ttgcacgatt taccatgaaa tcacattttg tttccattct
     3301 ctcacctcct gattctttac tccttttaaa ataatcctct ttataatcta ttgtttctca
     3361 ttcgatggtt gtgttatact cgtcttgtcg aggcggagta cgttgcacaa cttgttgtgt
     3421 aagcagtact ttgctttttt agtagcttgt tttctcattt gatatacttt gtaattttcg
     3481 ttcataatac tcaacctcct cctgataata ctccgggtct gccttcatat tttggagaag
     3541 tttgatctgt cttggattca tttctccttg ttcaataatc aaaggcaaca aggaagaatg
     3601 aaacgtttga tcgacctgtg caataaaaac taatgatggt gaaacaccat gaattaacca
     3661 actcatagtt ttctcaaaac tttgaggaat tgtatctaaa gaaatggtga ttgcgccata
     3721 attgctaata aaggctgccc atttggcatc aacttttgca cctagatcat ttggatcacg
     3781 gtcataaaaa caaattgctc cattaagtaa ctcaaagaac agtttggtta aatcatgggt
     3841 ttttgaaata atctttgcca agctttgcgc tttctctttt cgataccgta gttcaaagcg
     3901 attctttact tttgcctctt ctaaaggaat tcctcgtcgc ttccgttgtt catggtcctt
     3961 ctgataaaag caaaaacgac actgactctt ctttgagcct aaatacaagg tctcaccatt
     4021 tgattcattg tttatgatat ccgtaccgcc atgagattca ctcacttgaa aagtggtcca
     4081 cacatggcct agtattacct tatgcttaag ttctggaatg ctgagactcc ccacaaaatc
     4141 atctaaagta aaatcaatcc gtgtaaagtt cccctgataa tctaatacct tacgaaaaaa
     4201 ctgttgccaa tttgtttttc gtgtttttag ccatcttgct aagtgtttac agccttgacc
     4261 tgaaaactcc atcacagttc ctttaaccgc atcgtcaatg gaataccgaa tcgtgattac
     4321 ttgattccac acgtattggc caatatagcc ctttggcgca taatccagtt cctcaaaacg
     4381 ttcaatgggc atttcaataa tctcttcaat tagctttaaa tgttgattcg agaaaaagtg
     4441 aatggttaga taatcaatca aaacacggtt aactggaacg acctcttctt gcaaactatc
     4501 caacacaaga ttcaattttt cttcacttaa ttgtctcgtt cctttttcta ctttcgataa
     4561 taactatctc gaaatcccca ctttttctgc taaatatcgt tgtgtccacc ccctttcttc
     4621 tcgagaaact cgaatggctt caccgagatc atgttgatta acttggttca tcgacaaacc
     4681 tccttctgct atttacttgt caaagaacct attgaactta ttttaagtac atttttattg
     4741 tacgcctgta tttattttaa gtcaatatgt tttgactttt tttaagtaca cacgtatact
     4801 aaatttaaga acttatcctg aggaggagtt ttcatggaga tagacaagca agcagtcggc
     4861 aatcgaattc ggcagattcg ccaagaatta aagttgagta tggagaaatt tggcaaacta
     4921 attggtgatt taccgcgaag tacagtcaat aactgggaac gtgggatcaa ccttccaaaa
     4981 accgaaaccc ttcaccaaat cgcagagata ggtcatgtca ccaatgaata tcttttgtat
     5041 ggtgatcaag agaatcaata cattttagaa atgctccaaa aaaaggcagg taagcttgat
     5101 cctaagattg aaggtctcat agtagacgaa atcaaacacg ctgaccttgt tagtgaaaaa
     5161 gaaatggatc gaatgattga attttttatt acaaatctca ttccaccaac tgaacaagac
     5221 cagtttactt tccaatgtat cgatgaaaaa aagcagcttt atcttggttc aactaatttt
     5281 ggtaaggaag cccagctcta tttgcaacat gataatcaaa atcatattct gcacatgatg
     5341 ccctttacct tctccaactt tagtgtggat cgcatactcg tctatttagc aaatcaaaaa
     5401 tcctattcct attttgggaa acatttaccc aaggaactag gtgagaaagc catccttcta
     5461 tactcaatta atcaattgac cagcgatgtg cgaattgccc cgttggtcta ttcagaagaa
     5521 accgcttctt atcagtacac agaggacaat caatatctac tagaacagca atatctttat
     5581 ctaccattcg tgatggaagt cgagaaagaa cgcctgttaa atgcagcgta tccatcatca
     5641 aaatgaggtg acaaaattga taagcacgtg acaaagaaaa tactgctagg acactcccca
     5701 atgggtagtt gtcacagttt cctgttcttt gttagccccc tgttagaacc gggggcttta
     5761 ttcctgcagt ccggctacgc cttcctgcaa tagacaaaac tccagatcgg gtaattcgct
     5821 tgtggcgaaa cccgacctgg agtttgtctc tgtattttga accctaaaaa ttaatcaaac
     5881 agactgatat tctcataatc agcgcgaaat aagccattaa gtacagtttt cctacctatt
     5941 tttgataacc cgctggttag ttaatattcc ttttccgaga gagataaatg attggttaaa
     6001 tctatataca tctcaacctt taaaaatgtt ttcgaaatac ttcatcagta catgtcatac
     6061 aaattaccag tgcattctca aattctagtt ctttaattga tttcagttga ggagcagtta
     6121 cttttggcgg aaatttccat ttttgtccaa acctcttccc ccgatgaaat ttctttgttg
     6181 caattattgc aatgaaaaat ttttgtttca aaaatataca taattacttc ctccaataaa
     6241 aactatctac ctctcaaatt atttttctct aataagaaaa acagaaaagg taataagata
     6301 caacagtgat gattcctaat aaagaaaaga ctacaataat atttttctaa cttatcgcct
     6361 tcaaagaaat agtttcttat ttttcactaa aataaaaaat ttattgcaaa aaaaatagaa
     6421 gtgacaaaat tatcaatcat ttcaaggtaa tataactacc tgagtttatt atcgattaaa
     6481 atgctaacta catttgcaag agaagcgaga gttaaaggta aatatagaat ttctactttt
     6541 ttagcaatag aaataggaat aaaatattta aaatgtatag aa
//
LOCUS       DASJST010000144         6387 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.146, whole genome shotgun sequence.
ACCESSION   DASJST010000144 DASJST010000000
VERSION     DASJST010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.146"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            330..1175
                     /locus_tag="U4B05_002457"
     CDS             330..1175
                     /locus_tag="U4B05_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2051555.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="U4B05_002458"
     CDS             1456..2676
                     /locus_tag="U4B05_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN2051556.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="U4B05_002459"
     CDS             2673..5393
                     /locus_tag="U4B05_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2051557.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="U4B05_002460"
     CDS             5377..6321
                     /locus_tag="U4B05_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2051558.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DASJST010000145         6287 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJST010000145 DASJST010000000
VERSION     DASJST010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.147"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..966)
                     /locus_tag="U4B05_002461"
     CDS             complement(133..966)
                     /locus_tag="U4B05_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEN2051559.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(973..1659)
                     /locus_tag="U4B05_002462"
     CDS             complement(973..1659)
                     /locus_tag="U4B05_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051560.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(1665..2411)
                     /locus_tag="U4B05_002463"
     CDS             complement(1665..2411)
                     /locus_tag="U4B05_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN2051561.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(2404..2745)
                     /locus_tag="U4B05_002464"
     CDS             complement(2404..2745)
                     /locus_tag="U4B05_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051562.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(2924..3631)
                     /locus_tag="U4B05_002465"
     CDS             complement(2924..3631)
                     /locus_tag="U4B05_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEN2051563.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            3676..4155
                     /locus_tag="U4B05_002466"
     CDS             3676..4155
                     /locus_tag="U4B05_002466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051564.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(4396..4662)
                     /locus_tag="U4B05_002467"
     CDS             complement(4396..4662)
                     /locus_tag="U4B05_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051565.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(4673..4864)
                     /locus_tag="U4B05_002468"
     CDS             complement(4673..4864)
                     /locus_tag="U4B05_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051566.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            5155..5577
                     /locus_tag="U4B05_002469"
     CDS             5155..5577
                     /locus_tag="U4B05_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051567.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            5594..6016
                     /locus_tag="U4B05_002470"
     CDS             5594..6016
                     /locus_tag="U4B05_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2051568.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            6045..6245
                     /locus_tag="U4B05_002471"
     CDS             6045..6245
                     /locus_tag="U4B05_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051569.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
BASE COUNT         1857 a         1199 c          972 g         2259 t
ORIGIN      
        1 ccatccttat ttttagctcg ccaactatac aaaggttcgc tacattctgg acatttcccg
       61 ctttgcacta atactcttct tattagtttc tccatagcat ttgctaggct ttccatgtga
      121 tgcatctcct ttttaaattg gcaagtcgtc atattcacta ggattgctgt actgtagttt
      181 ttgactttgc tttttatgat tattcttgtc tgctttgatt tcgaatttga gcttctcaaa
      241 tttttctctc aatttcttag cacttctaat atttccaaac caaaattcat ttgtaggtag
      301 ccaattgatc acatactcaa tcgcttctat agacgcttta tctctttctt ccatcaacct
      361 gattgtgtct gcccattttt cgatatctac tttgttcatt tcttttggaa aatcttcagt
      421 taaattactt tgcaattttt tagcaaggcg taagtgttcg tcagaatact tacctttctt
      481 ttcttcttta tctatatcta tatctttctc tatctctatc tctaactctg gtgtagtttt
      541 gtctggacat ttgtccgaca cttgtcctcc agttattaaa ttccgttttg cctcttctat
      601 tttctttctg tattctcttt ttctatctgc ttcagttgag gattttccaa tgaaactttg
      661 tatatcagac atataaattg ctccgttatc taatacgtca ataagctgca aatcacggaa
      721 aatttgtacc gctttttcta cgactcctac agaatgtctt gtaatagttg cgagcattgt
      781 agagttaaat ggaatcctgt cattaaacat caacttacct tcgtgtttta gacttcttaa
      841 ataaagtttg agaagaatat tagaataaat atagccatct ggcatacttt ctaagagaac
      901 catctcgtca ctatcgaaaa aattctcttt tagttttaaa tagtagtagc gtttgttgtc
      961 agacaatatt ttttaccctc ctattctaag tttcttaatt gtttcctggt ttaacttgat
     1021 ccctttgatt tgatatttat ttttgaaatt gatcacacct atattgtgtt tctccgtgtg
     1081 atgaatcctg cagagtgctg caaatgtgta ctctgaatga tcaacttctt tgcgctttcg
     1141 tcttcctagc gctttgtcaa agtgatcgat gtcagctcct gttttgccac agatgcagca
     1201 gactcttttt gtgatgcatt tgtagaagta atattcttga ttcgctggta aaatctcata
     1261 gccttctttg aaaggaatat gatgttcaaa gatgaaatct aagatgatat ttgctaagac
     1321 attagcatca ctcacagttg tattcgattc gtctttgagg cttattttgc gccctgtgac
     1381 gccttcaaaa cggaagtaaa agaattcctt ccagaagtcc gttggcatgc ctgtatcgat
     1441 gaaaatatcg cctatcagcg catagatgaa gtttcgttgc tgcacagtaa atcgacgtgg
     1501 atcaataaat cgaacttcaa taatccgatc gccatcatat ccgtcataca tcgtctttag
     1561 tcgatcaatg ttcacttctt cattgatcgt tgcacctatg tcttttcctt tgaacttttt
     1621 cagaaccgct gaatatgaat cgatcaatgg tttaaacact catatcactt ctcttttgtt
     1681 tcttctctgt actgatcttc aatccagtta acgcctcgtt ttagaatgcc caagtctctc
     1741 ttggtccatt tactgtcatc agcggttata gaagccgcat cagtcaatgc aacaattgct
     1801 tcatcaattg atttttcgta cttgttagca accagttgta aagcatccaa gaatagcttt
     1861 ttgcttcttt gagtagctgg ttcaagcatc gagacatctt ctggcatatc ttcgccagca
     1921 aatatatata gccctagccc aaacattgct agatttttta caagacagcg catgatcgtt
     1981 ttgttgatat caaacatagt tgctgcttca actcgctttt cgatttttcc aacaatctct
     2041 tttttcttcg tttcgttatt ccactgataa tcattgactt cgtaggtata tggctcatct
     2101 ttcattgcct tgtttgcacc atccatgact ggtaaccaca tgtcacgctt tactccgttg
     2161 actgtgatac tggtaaaaac catatagcct gttttcttat caaagacata cggcagatat
     2221 gtttctggat catgatagat ttcataatct actgcctcac acactttgct aacttctgcc
     2281 caagcccatg cccacgaaag ataagtgagg ttatttcgtt tttcagtaac atctttcaca
     2341 tttattttgt ataaggtatt aaaaagcgta ttatcacttc tatttttcaa aggttgttcc
     2401 ttttcactca tcaaattctg cctccatttc agcaatgtat ttcttacctg atccgtaata
     2461 agagatatca atcaagttat ctctgtcata ctcttctagc gcatcaatca agccatcttc
     2521 gatgacatag atatattcag tttttttgga cttcctcgat aaatggataa gatagacatg
     2581 atcccaaata ctcacaaaat ttcccaaatc gtcttgatca catgctagtt cttcatccgt
     2641 caaaagatta cgtctgattt ttcgattgct tgtttccttg atattcgatt tgccccaact
     2701 aggatcagtc aaatattgat ctagagtgga aagttctttt ttcatgtggt aacatctcct
     2761 tagatgtatt ttctttgtga ctcattgctt tggtcggctg agtcactttt ttatttgttg
     2821 ccatgctttt tgcttatcaa tatgttgttg gcttaggatg tttggtttat tgtgtctcca
     2881 ccagcgatta gcaattatta cgcctatttt tagcgcttca gctctattca tcctctaaca
     2941 actccatttg cttaataatt gtcatcgtcg ctgtagaagg catccaattg ttaataaaat
     3001 cgataacatc atcgaactgt tttgccttga tacgatttct agctactgct ccagtgattc
     3061 gcttaattcc gccattcaag tctttaaaca attctgcttt tgctttgcta tttaaatgct
     3121 gctgattaca aataactcta attttgttgc gaaccatatt agaaattgtt cggtaatctg
     3181 gttcagctag aagttgattg ttcttcaaat cgtttaaatc agactcgata gcgtctactc
     3241 tttcattcgt ttcttcgttt gcagctagag caagtaatgc catttctcgt ttagttgctg
     3301 gaattgatgg caattggtct tttaattctt tttccatctt gttgaaggcc tcaatatatt
     3361 gaagtttaaa acttaaagct ttcttaccag tgaagcccat tgctaataga gagaagccgt
     3421 ctctattcat ataaataatt cggtatgatt gtttattttg aggatgaaca taagtatctt
     3481 cccaaaatag gtctgcccaa ttttcggcaa ccccctcttt tagttcatca atagccgtca
     3541 aaacatgtct gtgcttttta tcgaagcttt ctgcaacttg taagctcgta gtcacagctt
     3601 ctttattttt caaaattact aattcttgca ttatttcttc tctccttttt gatataattc
     3661 tggtggggtg aaaacatgag cagaaaaatt gataatataa tattgttaac aactattttt
     3721 tatttagtaa tcggagcaac tctttcctta ctgtcatttt tgtccaacag tagtttgatt
     3781 gtgttaaatc aaaaaagcat attaacagaa tttgcccaca tagattactt ctcttttttt
     3841 aaaactggac taatatgttt atttctatct tcatttatcc tctatatagg aactacattt
     3901 tgtctaatgg tgcttaattt aaaaatgttt ataattgaaa aatggatagt tattatcgca
     3961 aagattttct catcgatatc aattatcatt ccaatgacat tgacgttttc taaagagcaa
     4021 tttaacgtag ctactacatt cgtatctttt atagctctat tttcgtttgc tgttcctaaa
     4081 acttttcaaa gcaaatctag caacgcaaag gttaatgtag aaaacactga tagaaaaaat
     4141 gatgaaagtt attaaaaaaa ataattcttt catttattgt caaacctttc ttatagacat
     4201 tagacttttt tagtcagtcc ctcccgactg gctttttcgc tctgtactca gcttcatcaa
     4261 gcccaatgaa aatccaaagc atgtaaacga tcgtgccgat taatgcttgt ctactacccc
     4321 atagacctaa agcgtagata attagtggcg cgctgaatac taatgttcga ttaaactttc
     4381 ccatgttgac ctcctttaaa ttttgtattt agccatcagt tcatcgatgt cctttttgtc
     4441 atatcttaaa aagccatcta tacgaatttc ttttaaaccg tgagctatta acttctcaaa
     4501 accttgatcg tttacacctc ccacatattt tctagcttcg atctttttta aatatcgtgt
     4561 ttctgacaaa ttcttttttg aaagttgact aagtgcgtcc tttacaactt caacaattgc
     4621 aattcttaaa cgagtcaaaa actcttcgct tagaatattc atgacttgcc tcctatcgaa
     4681 ttctgtaatc acggatgatc tctagaatag ttctgtttgc ctttggtcca ctccagtgtc
     4741 catcaagaat ttgttgcatt cgtacacgcg tgtgaccata agcagtagcc aaatcatcca
     4801 tagttattcc attctctttc ataaactctt tgatcgccgc acgaccgtta tccaagtttg
     4861 acattaaccc acttcctttt tttataaatt tgtaagttaa aatgatagaa aaaatataat
     4921 ttccattgac atttttacac tatagagtaa aatgaataca tagagaaata agcctataac
     4981 aaaacctttt atttgcactt ggtcgccaaa ctttatgcta taaggtgtgt ttttagtttg
     5041 cttttttcta tcaaattaac ttacggataa ataataactc tatatcgtaa aattgtaaag
     5101 ctatttttac tctttttcgt aaattatttt ttcgtgttgg aaaggaacgt tggaatgaca
     5161 acatttgaaa gaataaaaga actagcgaaa aacaagaata aaaaccttca acaagtcgct
     5221 aaagatttga agtacggaga gaattacttt tatagtttaa acgcaggaaa acagccaact
     5281 gccgaaaagt tacaagaaat tgctgactac ttcgacgtat ctgtagatta tttgctagga
     5341 cgtactgaga aaaaaagata ctatgattta acagaaaaag acgagcgtga tatccaaaaa
     5401 gaattgcagt cattaatcga cgatttatct aatgctgatg gaatggcttt ttcgaagaaa
     5461 gataatgaaa tgagtgaagc gacacgtgaa gctctcatta tttctttaga gaatgctttg
     5521 cgaatagcta aaatagaagc taagaaaaaa tacacaccta aaaaatatcg cgattaaaag
     5581 gagtacgttg tttatgtatt tacctcaaat tgataacaaa attaataagc tggtaaaact
     5641 atatcaaaca cgcaatcctt ttagaattgc acgagaattg aagatactag ttttagaaga
     5701 agatttggga gaaatttatg gttactatag taaattcaaa cagataaaaa tgatccacat
     5761 aaattctagt ttagatgaga tgggaaaatg gataacttgt agtcatgaat taggccactg
     5821 tgttttgcat ccgaatgaaa acacacctat gttgtctaaa aaaacgctag tatctgaatt
     5881 aaaaattgaa aaagaagcaa attatttcgc aactcatctg actattgatc caactttaga
     5941 cggctttgat catatgacaa aatatgaaaa gctcgtctgc tttggattac cagatgaatt
     6001 cgatcgtttc ttgtgaatta taatacaacg taaggaggat atagatgaaa ataggaatga
     6061 gaaaaccaag tataaaaaaa tccataagcg ctagaaccac tggaaaagca aaaagaaaag
     6121 taaaaaaagc agttattcct ggttatggga aaaaaggtag cggctggatt aaagacccaa
     6181 aaaaggctgc ttacaataaa gtatacaaga aaactacatt cagtttttgg gatttattta
     6241 aataaaaaaa gcccgtgcag caacacggac tcgaaacctt atttcta
//
LOCUS       DASJST010000146         6242 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJST010000146 DASJST010000000
VERSION     DASJST010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.148"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(74..283)
                     /locus_tag="U4B05_002472"
     CDS             complement(74..283)
                     /locus_tag="U4B05_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051570.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(294..1621)
                     /locus_tag="U4B05_002473"
                     /pseudo
     CDS             complement(294..1621)
                     /locus_tag="U4B05_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1757..1912
                     /locus_tag="U4B05_002474"
     CDS             1757..1912
                     /locus_tag="U4B05_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051571.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(1986..2573)
                     /locus_tag="U4B05_002475"
     CDS             complement(1986..2573)
                     /locus_tag="U4B05_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN2051572.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(2656..3906)
                     /gene="clpX"
                     /locus_tag="U4B05_002476"
     CDS             complement(2656..3906)
                     /gene="clpX"
                     /locus_tag="U4B05_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN2051573.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(4153..5442)
                     /locus_tag="U4B05_002477"
     CDS             complement(4153..5442)
                     /locus_tag="U4B05_002477"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN2051574.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(5596..>6242)
                     /locus_tag="U4B05_002478"
     CDS             complement(5596..>6242)
                     /locus_tag="U4B05_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051575.1"
                     /translation="RKLIASTDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSE
                     ELPSLEPARQLASIDEIEQLPYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHP
                     VFYLTIEEKLVKVIPKDLPKPQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAI
                     VYPVYDTYIEDPKRWFQLTVEACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
BASE COUNT         1752 a         1272 c         1037 g         2181 t
ORIGIN      
        1 tcagtcaaaa aaaaaaataa aaaaccagtc agaaagactg gttttttatt tttttgtcta
       61 tcatcaagcc gttttatcgt tttttatttt cgcttcttgt ttttttcata taaacagaag
      121 caggttttct ttccttatga agaagataat aataaagtcc catcccgcca aacatgacca
      181 aaaaaccgat gactgatcga ataggaaaat taggaacaaa aaagagacag acggataata
      241 atgatgccgg aaccattatt cgataataga gttgtttatc cattttcttc acctcatatt
      301 ttttgtaaag taatcaattg ttctaattct cggtaatcta aaaattgcac acgcagatgc
      361 tgctcacctt tcttgacatc gaattggata cttcgccgct ttttctttat tcgtttgaag
      421 gtaacatcag agagtctatg tttgatttcc tttaacggta attctgacag ggattctgaa
      481 ggaacataga ttcttaagga taaaagatac tcttgatatt tttcatctgt tacctctact
      541 tctagatatt ccctaatata ataacaataa gcttcgattc cattatccaa agcaacagtc
      601 ggatcgataa ctttatagca catcatgcgt gatcgattct cgtcaaccca aggaaaatac
      661 gcctgagcat ttagtgttcc gcgaacacgc ctttctttat aatctccttg ttggatatca
      721 taactttttg ttacaaaaaa tgaagcattc tttttaggaa caggttgcat ttgtgcattc
      781 tgatactgga cagattggat cgtatggtca gtgtaaatgt attcaacgaa tggcggtttc
      841 aataaaccga caccattgtc ccaagcaaga tgatcaaacc caagctggtc cctaaaatag
      901 gattgaaaca tttgattgtt tggcttttgc cagttaaagg ggtctcgata aagctggata
      961 catatgactt cgttttctaa ttccttcact ttttctactt gttggatgtc gagaaaaatc
     1021 ggtttaggga tgatttgatg ataatagcga ctataataca tttcccaaca cgaagtgaaa
     1081 cataaacctt ggtaaaaaag atcgtatcct ggaaattggt tgcaatcgac tactacttcc
     1141 ccgttttgtt gtttcatttt aggtaaatgt tcgatttcat cctttttctc aaacaatagc
     1201 cgttgagaga tctgttttgt attgtgatag ataaactcgc tataagctcg catataggcg
     1261 aacaccccat gagcaatcat ttttttctga atgaagtctc gaatcaaaaa ttgcttttct
     1321 ttgaaaacag agatagacaa tacatttttt atcaataatt gattatcttc caagcgaaaa
     1381 ctacattcgt tctcgtcatt tcctacaact aaccaacttt cgtctcttac cgatgctagc
     1441 attatttttt cttgaagctt tcgaagatca tacggatccg cttgttgtgt gaaagagcgg
     1501 ataaatgttg gctggatcaa cagacgtgtc aaaatagccc aatcgcttat ttcctgtttt
     1561 tttcttcttc atttaagtct gtgagctgga tagtcatcaa taatttatct tcgccaatca
     1621 ttttctcacc tctttcaaat ctttttataa tttaattata accaatgata aagaaaaact
     1681 aaaactttat gtcttagaaa ccaatttgga cgattacatg atagactaag taaaaagaaa
     1741 caaagaggtg tttacaatgg ggttgaaatt tgaacgtaca gataatttag ataaactttt
     1801 cgaatcattt gctgtcgatc cggaaaaaac agttgcacca aaagaagaga tcgatcgatt
     1861 tctaaaacca cagccacaaa atgaaaagaa aaaagacaag aaacaaaagt aagttaagct
     1921 caaaaaaaga cttaccagcc aaacttttat gaaagagttt gactggtaag tcttttttat
     1981 tctttttatg attcaattgc ttgttcgatt gctgcccacg cttcttcttt tccctgtttt
     2041 gtaacggaag aaaagagaat aaacgtgtca ttgcgatcaa aatccaacgc ttttttgatt
     2101 tcgctctcat gcttgttcca tttaccacga gggatcttat ctgccttagt ggcaacaaca
     2161 attacaggga tatcataata cttcaaaaat tgatacatct ggatatcttc ttgagaaggc
     2221 ttatgacgca aatctactaa agaaacgacc gcacgtagct ggtctctagt cgttaaatat
     2281 gtctcgatca tctttcccca ttttgctcgc tcagttttgg atacttttgc atacccatat
     2341 cccggaacat cgacaaaatg taatgcgttc tcgatcaaat aaaaattcaa tgtctgggtc
     2401 tttcctggct taccggaagt acgggctaaa ttctttcgat caatcaatgt gttgataaaa
     2461 gaagatttcc ctacatttga tcgtcctgcc agagctattt ctggcaattg tgtatctgga
     2521 tattgttttg gtgccactgc acttatgata atttcagctt gatggacttt catggtctct
     2581 ctccttaaca aggtgatgaa taaagcaagc ggctgtcgca aaagccaaaa tgacttttac
     2641 ggcagcctct tttcatcaac ccgctttttt ctctgaatct ttatagatta ctgttggctc
     2701 accagacaat tcagctgctt cttttgtgat aatcactttt tcgatgtttt catttgaagg
     2761 gatatcaaac atcacatcca tcatgatttc ttcaatgatg gaacgaagtc ctcgagcacc
     2821 ggtattgcgt tcgattgctt ttttcgcaat ggctctcaac gcttcaggtt cgaattcaag
     2881 ttcggtatta tctagagaaa gaagtttttg atattgtttg actagcgcat ttttcggctc
     2941 agtcaagatc cgtaccagat catctgttgt caatttctct aaagctgcca taacaggtaa
     3001 tcgtccgata aactcaggga tcaatccgaa ttttagtaaa tcttcaggaa tgatatgctg
     3061 catgacactt tcgtcttcag aaagtttttg gttttgtgta ccaaatccaa tcgtcttttc
     3121 gcccatacga ttcttcacaa tcgtttcgat gccatcaaat gcaccaccta cgatgaacaa
     3181 tatgttcgtc gtatcaatct gaataaattc ttgatgtgga tgtttacgtc cgccctgagg
     3241 tggcacactt gcaacagttc cttctagaat tttcagcaag gcttgttgga caccttcgcc
     3301 ggaaacgtca cgagtaatag atacattttc gcttttacgc gcaattttat cgatctcatc
     3361 aatatagatg atcccttttt ctgcaagttc cacattgtaa tcagcagatt gaagcaattt
     3421 aagcaaaatg ttttctacat cttcccccac ataaccagct tctgtcaaac ttgtcgcatc
     3481 tgcgattgca aaaggtacat tcaatgtttt tgctaatgtt tgtgctaaaa atgttttacc
     3541 agaccctgta ggtccgatca agcagatatt gcttttttgc agttcaacat catctgtgtt
     3601 ttcttgtgct tgctgattga cacgtttata gtgattgtat acggcaacag ctaacgagcg
     3661 ttttgcgcga tcttgtccaa tcacgtagtt attcaatact tcaagaattt ctagtggttt
     3721 agggacttca gtgaattcgc gtacagcttc ttcataaaat tcttcatcaa tgatttcttt
     3781 acataggtcg atacattcat tacagatgta gacccctgga ccagctacga tctttttcac
     3841 ttcttcttgg gttttcccgc aaaaagaaca gcgtacggtc tcattgctac ttggattgtc
     3901 atacatggtc ttcacccctt aacttgcaaa aagtatgcaa gaaactatca aagactttac
     3961 agtcttttac tatcataaca tagtttagta aaaagaaaaa gtgtttaaca aaggatcaac
     4021 tgacctatac ctttgcattt tttactctta acaaaaaata aggaactgtg acaaaatcgt
     4081 gtcacagtcc cttatttcga ataaacaatt ttctagaagt agcttcttca gaaatgattt
     4141 attacttgat ttctattctt tcgcttctgt cgctgtacct gtgatcaatt cgattgcttt
     4201 tttcattgtc acatcatgtt ccagcatatc ttctgtcaac acgcgtttga tttgttcgat
     4261 tggcatgttg tatgtttctg ataattcttg gatttctgca ttgatttctt cttctgttgc
     4321 ttgaagattt tcagcagctg caattgcttc gatcacaaga tttgttcttg tgcgcatttc
     4381 tgcttcacct tcgaattgtt tatgcaagtc ttcttctgta gaaccagtta attgatagta
     4441 catttcagga gagatacctt gacgttgcat gttgttcaag aattcgtcca ttgaacgatg
     4501 tacttcatca tgaaccatca cgtgtggcaa ttcaacgatt tctgcgtttt caacagccat
     4561 acggatcgct gcttcatctt ttgcatcgtc cgctgctttt tcttttgttt ctactaattc
     4621 tttgcgatat ttttctttca attcgtctaa tgttgatact tcgtcatcaa catcttttgc
     4681 gaattcgtca tctaattcag gcacttcttt tgcttttact tcatggattg tcactttgaa
     4741 gactgcttct tttccagcaa gatcttctgc ttgataatct tctgggaaag ttacatttac
     4801 atctagactt tcacctgatt tatgaccaac taattgatct tcaaaacctg gaatgaatga
     4861 gcctgaacct aattcaagtg agtaattttc gccttttccg ccatcaaacg cttcgccatc
     4921 tacaaagcct tcgaagtcga ttactactgt atcgccattt tcagctgcag catcttcttt
     4981 gataactaat tcagcttgtg cttcttgttc acgtttgata cgtgcatcca cgtcagcatc
     5041 tgttacttca cggtcttgtt tttcaacaac tagatcttta tattgtccta attttacttc
     5101 tggtttcact gttacttcag cagtgattac ccagtcttga cctttttcca tgctttcaac
     5161 atcgattttt ggttgagaaa caggatcgat gccagcttct ttgacagcag cttcataagc
     5221 ttctggtaat acagcattca atgcatcttc atataaagct tcttcaccgt acatacggtt
     5281 gaatacttga cgagaaactt tccctttacg gaatccaggt acgttcaagc tttttttcac
     5341 tttattaaac gcagttgtta atcctttttg gatttcgtct tgcgcaatcg taaattttaa
     5401 aacaccatca ttcgtgcctt ttttttcaaa tgttgcagtc atttaattcc ctccgagttt
     5461 agaaattcat tttatacatc aaaaacgttt catgcatacc tttaaatagt acactaaccc
     5521 tttcaaattg taaacatatt gaatggatag aatccttgtt tttcataaca tttttatctt
     5581 tttcactgat aaaatctaat ggaagaagat catttgctgc aaaatttttt ctcttttttt
     5641 taaaaaatct tgtttttcat cttctgtacc ttcatacatc gtttttcctg tacacgcctc
     5701 tacagtcagt tgaaaccagc gcttcgggtc ttcgatgtat gtatcataga caggataaac
     5761 aattgcagat tgcaacgtga attcttcttt taacacggaa ctaagcagcg catcttcatt
     5821 cccgtaacga tctgaaaata cacataattg ctggtaactt gcctgctgct gcggctttgg
     5881 cagatctttt ggtatgactt tgactaactt ttcttcaatc gtcaaataga aaacaggatg
     5941 gcattcgttc aattgcctaa gtgtctccag taattttgcc cttgctaaaa tatggacctc
     6001 tggcgataca ataagtttac tcgctgtttg gataaactca tgataaggaa gctgttcgat
     6061 ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa ggaagttcct ccgatttatt
     6121 gtggagtgcc ttgatcttct gcctctccat ttgtccggat aattcttctg caaattggat
     6181 tttttcaatg acccgctgct cgaaagcttc ataaaaatct gtacttgcta tcaattttct
     6241 ag
//
LOCUS       DASJST010000147         6230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJST010000147 DASJST010000000
VERSION     DASJST010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.149"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..633
                     /locus_tag="U4B05_002479"
     CDS             133..633
                     /locus_tag="U4B05_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN2051576.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="U4B05_002480"
     CDS             730..2577
                     /locus_tag="U4B05_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN2051577.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="U4B05_002481"
     CDS             2580..3476
                     /locus_tag="U4B05_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2051578.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="U4B05_002482"
     CDS             3526..3915
                     /locus_tag="U4B05_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2051579.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="U4B05_002483"
     CDS             3902..>6230
                     /locus_tag="U4B05_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2051580.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag caaagcaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DASJST010000148         6175 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJST010000148 DASJST010000000
VERSION     DASJST010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.150"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="U4B05_002484"
     CDS             725..811
                     /locus_tag="U4B05_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2051581.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="U4B05_002485"
     CDS             complement(932..1621)
                     /locus_tag="U4B05_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051582.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="U4B05_002486"
     CDS             complement(1628..1906)
                     /locus_tag="U4B05_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051583.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="U4B05_002487"
     CDS             complement(1866..2780)
                     /locus_tag="U4B05_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2051584.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="U4B05_002488"
     CDS             complement(2762..3142)
                     /locus_tag="U4B05_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN2051585.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3594..3881)
                     /locus_tag="U4B05_002489"
     CDS             complement(3594..3881)
                     /locus_tag="U4B05_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2051586.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3901..4125)
                     /locus_tag="U4B05_002490"
     CDS             complement(3901..4125)
                     /locus_tag="U4B05_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN2051587.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4580..4750)
                     /locus_tag="U4B05_002491"
     CDS             complement(4580..4750)
                     /locus_tag="U4B05_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051588.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4765..5298)
                     /locus_tag="U4B05_002492"
     CDS             complement(4765..5298)
                     /locus_tag="U4B05_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2051589.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5319..6161)
                     /locus_tag="U4B05_002493"
     CDS             complement(5319..6161)
                     /locus_tag="U4B05_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2051590.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1634 a         1153 c         1041 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccaac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag cttactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccccactct ccacaataca
     3361 aaaaagacta gcaaatgcta gccacttttt gtaaagtttc gcttgtttca ccttcggtga
     3421 aattgttgca aatcaataat taggtagagc tacatttttt tcgttgttca aagattctta
     3481 tgccatttac tattaataaa tctataaata tgataatttt tcggctctat aatttatggg
     3541 actgatgaat cagaattgat tcttcagtcc cattttcatt ttattccaaa gaattaactc
     3601 caaataccaa tagaagccca tcctctatat acttgagatt ccttactaac aatttcttgt
     3661 aattctatta aagatttagg taatccaaat ttatgtttta aaatatatct tccaagaaaa
     3721 ctacttaatt tcatactaac ttcaccaaca ctttttccct cactgatttc tttagaataa
     3781 agaaagagct tttcttttaa gtcaggatag tcatctatag ttgcttcatt gtatacttta
     3841 ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca ttataataac ctccctacca
     3901 ctaccttcta ggtgcccatg gaccatgatt aacccaacca ttaacaataa tttttccaat
     3961 ttctccttta gcttgattcc aatctaccca acatttttct ttgttacaat aaagaccatt
     4021 tccatagtaa gtagctgcat caacttttat tcctgtaccg atgccagcta gacaagttcc
     4081 taatattcct agaataacac aatgttttaa tactttcttt ttcatgattg tttttccttt
     4141 tcgttttttt aaaattgctt gttatattaa ataatcttag taattttatt atataacatt
     4201 ttattatata acaatgatta acgtcataaa aagtagtttt gaattcataa tcgtttaaat
     4261 aaaattataa gatcagttat ttaaacagct agtagacata atgtcgatac taccaagtaa
     4321 cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt ttattggtct aggttaaaag
     4381 ctctttttaa ggcttggttt tctgcggttg tttctttcta attctttgtg aatattgatc
     4441 ggtcattttt taaatacttg ttatttttgt ttgtgtttgt tatgcttttt acatatctca
     4501 tattcattta ttattctttt ctaaggttaa taataggata atctctttca gaaagactgc
     4561 tcgtacgagc agtcttttct tatttatcaa gtttttctac aaatacttcc tcattttgac
     4621 tgttgtagta attaggattg agttttagtt cccctttttt atttcctttg ttggccactg
     4681 ataataacat tgggctaaag cttaatattc ctagtaggat agaaccgaat agcaagtgtt
     4741 tttttgtcat aagtatttcc ttcttcattt ccatagctgc caccattttt tccaattctt
     4801 tgtttgttcg tccattgttt ctgttgcttc agaaagagtc gttgaactat tctctgaaga
     4861 ctcttccgta taagtcaact gcagttgctc ctgaagtttt tcgatttgtt ggttggcttg
     4921 cagggtgagt tgttgttgct ggtcgagtag tttttgtata tgagaattat tttgctcttt
     4981 gagggcaagt tgttctcgta aaaattcatt ttcttcacgc aactgatcta gcaacttgtt
     5041 tgcaaccacg tttttgtttg gtatataagc atttttatcg ttgttttgtt catgtttatg
     5101 caacttatct agcaaccttt gattgtctaa gttgcgagca agttgcaata ctccagaacg
     5161 caatacaacc ttataaccac gctcattttt agctatcatg gaagaatcta atttttggat
     5221 atgtttcgat attgcttgcc tactcatctt aaaaagatct gacaattcct ttattgtaaa
     5281 ggtttcttgt ggttgcatac tggttgcctc ctaggttgct actggaataa attatctaaa
     5341 tcagataata aacttttttt ctctttttcc gaaagtgcgg actctgtttg tttttcaaga
     5401 gcttttttct ctttttctag ttgtttgatt tgttggagga gttcaagttc tcgtttcttc
     5461 tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt attctttttg tttatcagct
     5521 aaagccaatg ctttattttg gtcttctttg gttggtgcat gtttgttaaa gtcgtctgct
     5581 tttttgatct caggtttgaa cgtaaattga tagccaataa caggttttcc ccgtcctttt
     5641 ccgtatttct ttttgattgt tagtcctttg aagattgcag ttagttcttg acgtataggt
     5701 tttataactc gtttatctat atcaggttgc ttataacttt taggaatatc taataattca
     5761 agaaagtctt ctttggagaa atatgcgaaa cctttggttc tgtattgctt taacagacga
     5821 aacatagtct ttgcatagcc actttgtagc tcgttgaatt gttgtaaaga atagcgtacc
     5881 cactcgtcta aattatttaa caaaggcagt gctttttcgt aaattcgtat atctacgtaa
     5941 ggattttcta cgtccccgtt gattttaaat tctgtaaaca ttacaaacat atcgtaagaa
     6001 agcccagact cacttctacg accaaagcgt aaagataaga ttttttcata agttgctttt
     6061 aaatcttcta caaatcgtac atttgcagtt gctttgtagt tacttaaatc ttttagttgg
     6121 tcaaacgtaa aacgaactgt cttatcccct ttttcacgca ttctagacac gatgg
//
LOCUS       DASJST010000149         6028 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJST010000149 DASJST010000000
VERSION     DASJST010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.151"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(68..370)
                     /locus_tag="U4B05_002494"
     CDS             complement(68..370)
                     /locus_tag="U4B05_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051591.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="U4B05_002495"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="U4B05_002495"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="U4B05_002496"
     CDS             complement(1127..1900)
                     /locus_tag="U4B05_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2051592.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="U4B05_002497"
     CDS             2044..2394
                     /locus_tag="U4B05_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN2051593.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="U4B05_002498"
     CDS             2375..3091
                     /locus_tag="U4B05_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2051594.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="U4B05_002499"
     CDS             3091..4539
                     /locus_tag="U4B05_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2051595.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="U4B05_002500"
     CDS             complement(4610..5119)
                     /locus_tag="U4B05_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051596.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT         2051 a         1050 c         1170 g         1757 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaag
     5161 tgaaagggaa tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa
     5221 atcccttaag tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct
     5281 gttgatccat cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca
     5341 gttcagtagt ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact
     5401 ggaagcttac gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct
     5461 gccagaaaat aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca
     5521 attcctgctt gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata
     5581 gaaaaaataa actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa
     5641 gaagctctgt agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta
     5701 aagccgattg caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg
     5761 aaaataaaca atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc
     5821 ggattatttt catacttttc acctctataa aaagtataga aaatgaatat gaataaagta
     5881 tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag
     5941 gttgtgaaga agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt
     6001 cgaaaagagg agcagcgaca ataatttt
//
LOCUS       DASJST010000150         6003 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJST010000150 DASJST010000000
VERSION     DASJST010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.152"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..518
                     /locus_tag="U4B05_002501"
     CDS             174..518
                     /locus_tag="U4B05_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN2051597.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="U4B05_002502"
     CDS             519..938
                     /locus_tag="U4B05_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN2051598.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="U4B05_002503"
     CDS             complement(1030..1380)
                     /locus_tag="U4B05_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051599.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="U4B05_002504"
     CDS             complement(1346..2677)
                     /locus_tag="U4B05_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN2051600.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="U4B05_002505"
     CDS             2933..3499
                     /locus_tag="U4B05_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2051601.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="U4B05_002506"
     tRNA            3644..3731
                     /locus_tag="U4B05_002506"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            complement(3783..4988)
                     /locus_tag="U4B05_002507"
     CDS             complement(3783..4988)
                     /locus_tag="U4B05_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2051602.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5126..5756)
                     /locus_tag="U4B05_002508"
                     /pseudo
     CDS             complement(5126..5756)
                     /locus_tag="U4B05_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
BASE COUNT         1992 a         1031 c         1115 g         1865 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatatca acgattagac acgatttcga tcgtgtcttt tttgttgtat
     3781 atttagccta aaatgttttt tgactcccct tttgactccc cctgagtact gttcttcata
     3841 aatgctacaa aattattgat gacttttttg ttttgagttt cagttacatg agtgtaaata
     3901 tttgaagtag tttgaatgtc tgcatgccct aaacgttcct gaacatcttt taaagaagct
     3961 cctgattcaa aaaggagaga ggcatgagta tgtcgaaaac catgagtagt gattctttta
     4021 gtcaattctg gatgatgctt atatatgaca ttaagccacg aacgtgaaac tgtagggtta
     4081 tagagaagac catcatggtg aaaaattaag ttttcccttt ggattgtaac gatcgttttg
     4141 ttttttgtat aatactttct caataaactg atcaaatcat tgtccagata aatttttctt
     4201 tttccagatt ttgatttagg agtatttacg atcaatcttc cttgataacc tcttgttaca
     4261 gttttattga tattgagtgt tttgctctta aagtcaatat ccgaccaggt cagagcaaaa
     4321 gcttctcctt ttcttatacc tgtaaaagcc aatagggaaa agaataaata tctttcatca
     4381 tcatcgtttc ttattgcttc taaaaatttg attaattctt ctttagtata aaattcgata
     4441 tttttatctt cgatatctag agcttctcca cgtggaatag aaactaattt cataggattg
     4501 ctagttataa tttgtaaaga agcagcgtaa tcaaaaacat tagatgtgta attttttatt
     4561 ttcttaaaaa ttttaggatg gctatcggac caagtgttta ctgcttcttg acagaaaaaa
     4621 acgtctattt tgtcaatgaa cttatctcca aacactttta agatgtgtgt gtcaaaaatt
     4681 tgtttggtgg aggaccaagt gctttcttta actgtctttt tataattttc aaaccataag
     4741 ccgtaaacat attcgaattt cttgctcttt tgagctgtag aagcttgcag tcctttttcc
     4801 tgtatatctg cctccaatct ttttaaagct ctctcagcgg ctaatggcgt actaaaacct
     4861 cgtctcgttg tttttctttt ttttcctgtt agaggatcaa ctcctaagta cagactaaac
     4921 tgatattttt cttcgccttt ttttgttaag tgctttttaa ttcgtttatc aatttctttt
     4981 tttgccatct ttatctttcc tttcgtacgt ttgttcggtt gtacagcgga tttcgagatg
     5041 gtaaaatagg gtacaacaaa tagacctact ttaccgtagc tctttgcaca tttgcgttct
     5101 tggtcgggcg gcaaatgtgc tttttttatt tcactcttag ttcttgtcct ggataaagca
     5161 tgtagttgtt tggatccata ccgtttaatg caaacagttg atctaccgta attccagctc
     5221 gttcagctat ttgtttggtc ccttcaccag gttgcaaagt caaggtttcg gtttcagatg
     5281 aagaaggtgc cgttggttga gcttgattag ctggtacata atcttgtggt actgattgct
     5341 ccgttgaact gggagtgggt gaaacttgtt caacacttga actaggagta gcttctgcat
     5401 agttttctac actttcagca gagtaagtac tactagtttc aggaggcgta ttctgcgcaa
     5461 tagcagcttg ttctacagta ctagaagatg caatagtact ttctgttgta gtcgaagatg
     5521 attgttcgtt gctacttgtt gagctagtag ttgttctaga ttctttagtt tttttccttt
     5581 ttgtagggtt tcaaaactaa tttctcatta tcatcagatt tgttaggctt ttctggagtt
     5641 aaaataatat ttttatcttc tttgctgaca gtatatttgg catcattatc tttgtcttta
     5701 tcatctttcc aagtcataac atcgccttca agtgtatact caaatttgta attcatttgg
     5761 tcgactaagc ttttagcaaa ttcttctccc atagcctccc aatcgttact tgaagttgat
     5821 ttcatagaac ttgtgtcaac actaaaagat acgacgtgat cagaaaatga tacgatcata
     5881 ttaggttcgt catctttggt tgcttcaatc aaccaatcat tagcttttaa atcttctgta
     5941 gttacttttt taccacaagc agtgaaaagt aacagagaag caaaaactaa taaacttgtt
     6001 att
//
LOCUS       DASJST010000151         5954 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJST010000151 DASJST010000000
VERSION     DASJST010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.153"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..813
                     /locus_tag="U4B05_002509"
     CDS             <1..813
                     /locus_tag="U4B05_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN2051603.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="U4B05_002510"
     CDS             807..1229
                     /locus_tag="U4B05_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051604.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="U4B05_002511"
     CDS             2399..3367
                     /locus_tag="U4B05_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2051605.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="U4B05_002512"
     CDS             3384..3908
                     /locus_tag="U4B05_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2051606.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="U4B05_002513"
     CDS             4284..4565
                     /locus_tag="U4B05_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051607.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="U4B05_002514"
     CDS             4567..4983
                     /locus_tag="U4B05_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051608.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="U4B05_002515"
     CDS             5613..>5954
                     /locus_tag="U4B05_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN2051609.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DASJST010000152         5882 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJST010000152 DASJST010000000
VERSION     DASJST010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.154"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..191)
                     /locus_tag="U4B05_002516"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="U4B05_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            212..1414
                     /locus_tag="U4B05_002517"
     CDS             212..1414
                     /locus_tag="U4B05_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HEN2051610.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2147..3115
                     /locus_tag="U4B05_002518"
     CDS             2147..3115
                     /locus_tag="U4B05_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2051611.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3126..3941
                     /locus_tag="U4B05_002519"
     CDS             3126..3941
                     /locus_tag="U4B05_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN2051612.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4009..4719
                     /locus_tag="U4B05_002520"
     CDS             4009..4719
                     /locus_tag="U4B05_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2051613.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4755..>5882
                     /locus_tag="U4B05_002521"
     CDS             4755..>5882
                     /locus_tag="U4B05_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN2051614.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2019 a          884 c         1188 g         1791 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 agggtcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 gtggcaacat tgtgtacttt tctttgcaat cttgtgtaca aaactattgc aaaacacagc
      241 gcataagaat ccttacaaaa cagtagatga agcaacccaa ttagatcttg ttatagttgt
      301 taatcagctc ttaacgggtt acgattcaaa atgggtcaac accctatact tagataagtc
      361 acttgaatat cagaacataa ttcaggcttt ttcacgcaca aatcgtctta atggtcctga
      421 aaaaccgttc ggaaccattc gttattatcg taaagcacat acgatggaac agaacattga
      481 agaggctatt gagttatact caggcaaaca agcaaaaggg ttatttgttg aaaaattata
      541 taataactta gaaaagttga acgatatatt cgaagaaatt aagtccgtat ttaatatttc
      601 tggcatagaa aacttcagtc atttaccgga ggttccaggg gaaattaagc aatttgtaaa
      661 tctttataat caactaatcc attgttatga atcagcgatg atacagggat tcacatgggc
      721 aaaatcgact tatgaaatcc aggattatga acctataaca gtagtaatca cagaatatga
      781 atttaatatc ttggcacaac gttacagcga gatagagaga gaacctagag acggaaatga
      841 tagtatagcg tatgacattg attaccaatt acctgaaggt agtcgtagtc gtattaatta
      901 tgagtattta aacacaaact tcaaaaaata catgaatata aaagaagaac gtgattcgaa
      961 tttaattcaa tcattgttaa acgacttaca tcgcacattt gcgcaattac cagtggagtt
     1021 acagccatat gctgagatga ttctaaatga tgtaaataat ggggaattag ttatcaaaga
     1081 aggatgggaa tttactgatt acctcaatga atatcagctt agtgaagagg acgattttat
     1141 ccaagattta gtggattcaa ccaatattaa tgaggcatta ttacgtgaga tgttggattt
     1201 acgcttaacc gaggtaaaca ttaatgaata tagtcgattt gataaattaa aatcaagtgt
     1261 taacgtaggt catttcagtg gatatatcga gaaaaattta ggtaaaatgg taataccaat
     1321 caaggtaaat atgctaattg acagattatt gcgtgcattt ttattagaag atgtattcga
     1381 tgttacagag tacataaaga attactttaa ctagagtaca cctacatctt atagtacata
     1441 aaaaaattca tcttttttag tttaataatt caccaaaaag gtgagtaatg ccatttttga
     1501 aaattgagct tgttttatgt ctacatctgt atccgtatat aatccaaagg attataaact
     1561 cagaagagtc tttatttttt ctgaatataa ctactgtatc aaaaacaatc gattatgaga
     1621 caaaacaaaa tgacctcttt tatttaagaa aggaggtcat tttttgtatc ataaattgta
     1681 ttaaaatcaa tgtatcactt tttaatcaat tttaagacca taaattgcgt actaaaagaa
     1741 attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc acccatctac
     1801 ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg tgcaaaacga
     1861 tttcttctaa acaaatcaag acaaacttat aaaccttcat cccagcacca taattattta
     1921 aatcataggc atttttcgaa attagtatat gtactcaagt ttcactgcag attatttata
     1981 ttttttagag aaataaattt ttcctattga caaacacaaa gaaagcggat acaatacaat
     2041 tatgtcacat gacgttatat gacgttatgt aatatcttta catttaatca gttactttta
     2101 ttttggataa aattaacatt tttaaaattt taaggaggaa atgttgatgg atgtagcaaa
     2161 aacggttaac gcaattgtag aaaaaaatga tggagagatt tctggaattt atttggtggc
     2221 ttgtggcggt tcgctggtag atatgtatgt ctcagattat tttcttaaag ccgaagcatc
     2281 gaagtgtatt acaggcttat atacagcgaa cgaatttaac catgtaaccc caaagcaatt
     2341 agatgataaa tctgttgtca ttctatgttc tcatggagga aatacagcag aaacggtaaa
     2401 agcaggagaa gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc ataataaaac
     2461 tgcggattta ttaaaggttt ctgatcatag tttcctttac gaatggggag atgatgcaaa
     2521 aatagtcaat aatcctatgg ccatcattct tgatttaact gtatctttgg tcaatgttac
     2581 cgaaacattt gaacattacg gtaaattcag agaaggcatg aaggttatcg ataccgtaat
     2641 tgaaaatggc attaagcagg tgcaggatcg tattaaagtg tttgctgata agtacaaaga
     2701 tgaaacaatg ttttatatct taggtagtgg accatcatat ggacatgctt atggtttttc
     2761 aatttgctct ttgatggaaa tgcaatggct tgattcaact tctgttcatt ctggagaatt
     2821 cttccatgga ccttttgaag taacagacaa agatacgaat tttattctct tgatgagcat
     2881 tggacgcact cgtgctttag atgaacgggc aaaacgattc cttgataaat atgcagaaag
     2941 agtagaaatc gttgatgcaa aggaactagg attggatatg atatcagatg aagtttctga
     3001 attcttcaat cccattttat tctacagtat tctttcagaa tatcgttcag ctttagcaga
     3061 tgcacgcaat catgatttag atgttcgtcg ctatatgggg aaagtggagt actaggagga
     3121 ttaaaatgaa ggtattaggc ttaggtgata acgtcgtcga taaatatgaa aatctgggaa
     3181 ttatgtatcc tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa ctgggacatg
     3241 aaagtagctt tatgggagtt tttggctcag atgaggaggc agctcatgtt ttatcagcaa
     3301 taaaaaaaat aggactggac aacagtcact ctcgctttga aaaaggcgag aatggctgtg
     3361 ccagagtgaa gattgatgag ggcgaccgga ttttccttgg cagcaattcg ggaggtgtta
     3421 ctcgtgaata cccgattcag cttactgaag aagacagaga atatttaaac cagtttgaat
     3481 tgattcatat gggattatat agccatgtca atcatctatt agaagagtta caaaatgttt
     3541 ctggaaagat ttcttatgat ttttctgacg actttacgga agaagaggtt cagcgggcaa
     3601 ttgataaagt tgattttggc ttcttttcta tagaagactt ttccgatgaa gaagtaaagc
     3661 agtttttgat gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc cgaggagaaa
     3721 aaacggctat tggttatgat ggagcaaagt tttatgaagt gaaccccgtg ctagaagaac
     3781 cagttgatac aatggcagcg ggagattctt tcttaacagc tttcctactt tattttctcg
     3841 aaggggacga tatagtcacc gcgatgaaaa aggggaatga atttgctggg aagtcttgtc
     3901 tggttgacgg ttcgttcggc tttggtattc attacgattg agcttgtttt aaattcctgc
     3961 atttgataac ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat gctggataca
     4021 aatgcacaat taccattgta cttacaatta aaagaaatat taaaaaataa aatcaataca
     4081 ggggagtatc ccgagaatac gaaaattccc gctgaatcag agctgataga aagttatgag
     4141 gtcagccgaa tcacagtgag aaaagcagta gaagaactgg tagaagaagg gtacctactt
     4201 aaaaaacaag ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa aattgaatat
     4261 gttatgggtt tcagtgagag ttgtaaatct aatggtttta caccgactag cattgttttg
     4321 gaacgcaaga tcattccagc aactgaaggg atggcaaaaa aactcgatat cgctcaaggt
     4381 gatgatgttt tatatatcca gagaaagcga atggcggata acatccccat tatgttagaa
     4441 aacaattatt tttcttatga aagatatagt ttcttaatgg aggaagagct gactgattct
     4501 ttgtatgaag tacttaaagt ccaaggtacc ttagccatca atcctgggga aactgcactt
     4561 gaaattgtca cagctgatga ccaaattgcc aaaataatgg aagtagctgt tgggacgccc
     4621 tttttcttta tggatacttt aatcaacgat caaaacaaca taccaattca tgtaggtaat
     4681 caatattacc tgggagaagt atatcgtttt tacttatgat ttcctgtttt gatttcaagg
     4741 agtagatttt atttatgaag caagtattgc ggtcaaaaat gatttttgat ggagagcagc
     4801 cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg gaaaacaact
     4861 ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat gatcaattcg
     4921 ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct atgaatgctg
     4981 gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt aaagctgtaa
     5041 tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct gccctgtgga
     5101 aaactgaccc atcagaagag ctattgaatg aaataaacca ctttggtcca atcacagcga
     5161 tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact tatggtttta
     5221 cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg ctagcaaaca
     5281 aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca aaaggtgtta
     5341 tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata cctcagagga
     5401 aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat gaacttctat
     5461 atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg aagatcatgg
     5521 tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag tcaggagtag
     5581 cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc aatgaagtag
     5641 agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct gcgtcaattc
     5701 ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt gatttggaaa
     5761 tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca gaggtatatc
     5821 cacaaattct cggcttgaat caaacgttag aagaggctta catgtcaact gttataaaaa
     5881 at
//
LOCUS       DASJST010000153         5870 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJST010000153 DASJST010000000
VERSION     DASJST010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.155"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(289..657)
                     /locus_tag="U4B05_002522"
     CDS             complement(289..657)
                     /locus_tag="U4B05_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN2051615.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            807..1289
                     /locus_tag="U4B05_002523"
     CDS             807..1289
                     /locus_tag="U4B05_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN2051616.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1326..2336
                     /locus_tag="U4B05_002524"
     CDS             1326..2336
                     /locus_tag="U4B05_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN2051617.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2451..3164)
                     /locus_tag="U4B05_002525"
     CDS             complement(2451..3164)
                     /locus_tag="U4B05_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051618.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3354..3944)
                     /locus_tag="U4B05_002526"
     CDS             complement(3354..3944)
                     /locus_tag="U4B05_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN2051619.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4428..4655
                     /locus_tag="U4B05_002527"
     CDS             4428..4655
                     /locus_tag="U4B05_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN2051620.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4671..5837
                     /locus_tag="U4B05_002528"
     CDS             4671..5837
                     /locus_tag="U4B05_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEN2051621.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNDNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
BASE COUNT         1987 a          947 c         1143 g         1793 t
ORIGIN      
        1 tgaaaataat gggcagtatt atgaaagatc actgatattt tctcgtattt tttccttatt
       61 caaaagtttt gcaggaaaat tgctgaaaaa ataattttac taagtagaaa attcaaagcc
      121 attcaatcag tgattttttt cagcacaaga aagactcctt ataaaaatag cagatacttt
      181 gtcaattaag tgtttaacca gggatgataa aaaggatagg attgaacaaa acctgtataa
      241 ggtgatgaat agtttcctcg ccagtaaaag gtttattcgt ataaagcttt atcgccaaaa
      301 tttatcatga ccttttttat cttcaatatt aaatgaaatc aattgttcaa tcaattccca
      361 gttgattaca cttttccaag gaactttgat gacgttatcc gtatatacgt agcctgcttc
      421 ttggatattt ttttcaaggg cacgaatcgc tgctttttcc ggtgcgatag aaaaatgatt
      481 tttagcaaaa ctaaatgcaa taataaaggt accatggtca gtaaacatag gttggttcca
      541 ttttacagtt gtgtctagct ggggatacgt ttctgttatc ttagcaaaaa ggtctttgat
      601 cttttgttga tgttcttttt ctttgatttg attgatgatc ggctgaaatt tttccattct
      661 aaaacgcctc cattaagtgg tttgatttat tttaccagaa aaacaatagt tatgtataaa
      721 aaacgaacaa aacacaagta atgttgtaac cgctatattt atatgataag atgtctcaaa
      781 taataaaata gaaaagagac ataaaaatga ttgacttggc aaagaaattc ttacacgaaa
      841 atatatattt tgagttgtat catcatcgag ccatttatac aaatgaagat gctttggtaa
      901 taaagaaaga acaaggattt acaggtacag agaccaaaag cttattctta aaagataaaa
      961 aagataataa ctacgtatat ctgactttta ctacgaaaca aacggatttc aaagcattag
     1021 ggaagatcgt cggtaaaagg ctttctattg tttcatcaga acgcatggaa gaacaaacgg
     1081 gacaaaagca gggcgctgtt tctccgtttg gttatgaaaa acagattccg attattattg
     1141 atgaagagct cttatcaaat gaaaaacttg tttttgcacc aggtagacca gatcaaacga
     1201 tggtcatcca aacggttgac ttaaacaaaa tcatccagct acttgagatg ccgatatttt
     1261 tgatgccggc aaacgaaaaa aacaattaac caagtaaaat agtgcatact tggaggtgaa
     1321 gcaacatgat aagttatcca ttattagaaa gaaatgcaac gattggggtt actgcaccat
     1381 cctcaggcgt atcagaggaa caccattcgt tactcgaatc agctttaaag agaatggaag
     1441 aaaaatcttt ccaagtagtt actggaaaga cgctttggac gcaagataag gaaaaatcag
     1501 gtccagcaag aaaacgagca gatgaattca atcaaatgat gcaagatgaa aatatccaaa
     1561 tgatctttcc gacttgggga ggagaattat tgattgagat tctcgatttt gtcgattttg
     1621 atgctctaca accaaaatgg atagtaggtt attcagatat tagtacgttg ctacttgctg
     1681 tcacattaaa aacagggata gccactgcac atggaactaa ccttattgat ttaagaggag
     1741 agtacttgga tgaaacgaca gcaatgtggg aaagtgtact gaaaacaaga gaaggagaat
     1801 cgattctaca gcaatcttct gaaaagtatc aaaaagtgtg gccccatgag cattcatcat
     1861 cttctgtttt ccatctcact gaaccaacaa attggcaatc agtcagcgga gatgcagtaa
     1921 ggatgaaggg acgattatta ggtggttgta ttgatacgat caggcattta atcggtactc
     1981 cttatggaga tgtccaatct tttaaaagaa agtataatga tggaaagccg atcttgtggt
     2041 ttctagaaaa ctgtgagcta tcgtcagttg atttaagaag atcacttgtt cagatgaaac
     2101 tggcaggctg gtttgatggc agctcgggga tcatgttcgg tagaagtgcg gcagaggatt
     2161 tgttagatta taaaatgaag gatgtatacg aagatctgtc aaaggaatta aaactaccga
     2221 tcttgtatga tattgattgt ggtcatgtac cgccacaact gacattgatt aatggtgcct
     2281 atgcagaagt agcggtagaa gatagaaaag cgactgtttt gcaaacgttt atttaaaaaa
     2341 ttttttcctg ccttggagtc tatcaaagac caataaatct aaatagaaaa gaaagtgaaa
     2401 gaagctgaca aaatgctgtc agcttctttt tgtataacat attagaaagt ctaattatct
     2461 ataactgtca catcgattaa ctgtttcacg tcattgggat ttcctgtagg aatagctact
     2521 acagatgcat tatcaatgat ttcttgagtt gtttctctaa tgaacgctgg ataagatgtt
     2581 tgatctgcag gcatttgcca atgtgaagga actggataac tagtgattgt cccgtcatgg
     2641 ttacttgaat acactaaaac tccctggtaa ctgtatttcc cagtgagcat aaccacatct
     2701 ttcggataga ctgcactccc tgcatcataa ggggcgatag gtgttcctgc aggataatga
     2761 ataacatgta attcgaaatc acccgatgta tctctaaaag tattacctct aatagctagc
     2821 caaacacgtg cgtactcaat ttgtagatca gaatatcctg ctaacggatc tattgactga
     2881 gaagactgtg aggaggttgt gtctattgtt gaagaagaaa tatttgtctc aactgtacta
     2941 ctagatgtag aagaagtatt gggctctgag actttactgc ttgttgaaga tgtattcgtt
     3001 tcagtagaag ctgatttctg agtacttgat ttttcagctg aactggtttg tctgattgaa
     3061 cttttgtctg tggtcgcaga tggatcacta ttttctttac taggagacgt acatcctaca
     3121 aggaataaag agaccaacaa taaaataaaa cctaactttt tcatataaaa tacctcatta
     3181 tttttttaat accagtatag cataatcctg aatttttgaa tccgtaaaac cttgtctatg
     3241 aatataaata gagtgtatct tctttgataa atgtcagaaa aagtgtgaca aataatcatc
     3301 agtaaaatta cttgtcacac tattgataag gagtatttaa ttgaagagaa tgattaggag
     3361 aaaggatctc cgtacttttt atagtaatca tagaatccaa gtttgtatag acgaatcgtt
     3421 ttttctctta tttcttctgt ttcaaaatgg actgcttgaa gaatcaagtc tgtgaattct
     3481 ataggagctg ttccattcgc agtaatcaaa ttacgatctt tcacagcttg ttgttctata
     3541 aaatcttctt tattctgata attttccgat tctaaccaag gaaattgcgc atttccggtg
     3601 tgtttgtaac cttgcaataa tccgctctga gctaaataat caactgcccc acaaatagca
     3661 gctactggta cgtctgtttt taacaattta tcgattattt gatataaatg atgatttttt
     3721 atgtcccatg aattgccacc gatcataata aaaaggtcaa ctttttcagg tatttgttcg
     3781 attaagtaat cgatttttgt ttttaatcct ccaatggaag tgacctctga ttggatagaa
     3841 gcagttttgt tttgccattt ctcattttgg tttagtgtgc tagtaatata agcaccttcc
     3901 caatcggcat attcctctaa tagaaacgta atcgcagttt tcattttcaa gctctccttt
     3961 actatctata acaaggacaa cagttttaac ctacagacat tcattaaaat tgggtagcga
     4021 cagcgaaaac aacaattctt aagttttaga cagagtatac agatagtcaa cttaattaaa
     4081 tacgaactaa ataaaaatga caggtaatca tttttgttta ccagcattat aacattttgc
     4141 atacatggat gacaaaagaa ccaattccca cttgcttgag tacaatatta aaagaaactg
     4201 gaaggcaaaa agtaattata gaaacgaaaa ataaatcata tcgggcactc agacatgaaa
     4261 acaaccctag agatatataa ctaggtaacc agtttaatga aagaaaaaat catccaagaa
     4321 gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat gcgtatatga
     4381 gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg aaaatagctt
     4441 ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt accgtttatc
     4501 gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt ttagagctag
     4561 gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta
     4621 agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca atgatagaaa
     4681 caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt tgcgagcata
     4741 tctgctatta ctgtgatttt aataaagttt ttttagaagg gcagccagta gatgagtatg
     4801 tggaaatgct gctgaatgaa atgaaaataa tgctagaaag tcatccgaca gcgaatttag
     4861 aaacacttta cgtagggggc gggacaccaa cttctttatc tgctaaacaa cttgatcgac
     4921 tgttatcagg tgctagagag atcctgcctt ttcaaaatga taatgagttt actgtagaag
     4981 cgaatccagg tgatttgacc cgtgaaaaac ttcaagtaat gaaaaactat ggcgtcaatc
     5041 gtttatccat gggagtgcag acatttgata atcgcttatt gaaaaaaatt ggacgtaaac
     5101 acaccgcaga agatgtgtat caaactatgc aatttttaga agcagaaaat tttaccaatg
     5161 taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat ctggatactt
     5221 tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg atcctcgaaa
     5281 acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct gatcaggaaa
     5341 cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga cgtcaccaat
     5401 atgaaataag taactttggg ctctccggac atgaatctca acataacctc atgtactgga
     5461 acaatgccca ctattttggg tttggagctg gtgctagcgg ttatcttgga gatattcgtt
     5521 accgcaacaa aggaccaatc caacactatt tacgcccatt acgagcaaat gaacgcccgg
     5581 tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg ttcttaggac
     5641 taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa aaaacaattg
     5701 aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt gttaatgaag
     5761 aagaccgtgt ttatttgaca ccaagaggga cctttttggg gaacaatgtg tttgagcgct
     5821 ttttgtttga aaaatagttt aaagaatatt tgttttttat ctgaatttaa
//
LOCUS       DASJST010000154         5604 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJST010000154 DASJST010000000
VERSION     DASJST010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.156"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            160..2220
                     /locus_tag="U4B05_002529"
     CDS             160..2220
                     /locus_tag="U4B05_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN2051622.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2303..2872
                     /locus_tag="U4B05_002530"
     CDS             2303..2872
                     /locus_tag="U4B05_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2051623.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3047..3853)
                     /locus_tag="U4B05_002531"
     CDS             complement(3047..3853)
                     /locus_tag="U4B05_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051624.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3991..5505
                     /locus_tag="U4B05_002532"
     CDS             3991..5505
                     /locus_tag="U4B05_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN2051625.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1995 a          968 c         1131 g         1510 t
ORIGIN      
        1 agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa aaatactctc
       61 ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat gaaaattact
      121 acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa aagacgttca
      181 aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac
      241 tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt ctatatgcat
      301 gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt ttctgagacc
      361 actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa acagctactt
      421 cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca ctactggtta
      481 attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct ttctatcttt
      541 tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga tattttgaat
      601 catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt atatgaaaaa
      661 gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa agtttcgata
      721 tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat gtccaaagta
      781 atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac tatttccttg
      841 tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt
      901 gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga gagttacgcc
      961 cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa
     1021 atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt tgtattaact
     1081 tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta tgtagaagca
     1141 tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa taaagaactt
     1201 caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct acctgctgaa
     1261 ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg gaaagtcact
     1321 tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg gtcggagatc
     1381 cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg ggaaattacc
     1441 tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct tattgttcat
     1501 ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc cttaggtaaa
     1561 cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga ggatggttat
     1621 ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc gtattcactc
     1681 caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca gatagatcaa
     1741 aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat ggctcatgca
     1801 gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag aatatctaga
     1861 aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc acaagtttct
     1921 ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa agaatttgtt
     1981 gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc
     2041 ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact tcctagtccg
     2101 ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt catgcgaggt
     2161 atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga ctggttctga
     2221 tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat ttggcataat
     2281 ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag aaatctggat
     2341 gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt ttgaatttcg
     2401 attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg ttggggaatt
     2461 tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt atcctgaaga
     2521 acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag caacctgtac
     2581 cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt tagcagtgaa
     2641 accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct tgcaagaatt
     2701 tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga gccatcaggc
     2761 gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag acggcacacc
     2821 taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg
     2881 tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat atcttacttt
     2941 tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa gaaggggcag
     3001 tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat cgtttgattt
     3061 ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga ctatagaggt
     3121 aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc ttcatagtat
     3181 ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt ttaccatctg
     3241 cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca gtgtttccgt
     3301 ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc atatcgaatt
     3361 gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact gtcatcgaaa
     3421 aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt gtatttttat
     3481 tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg aatgcatgta
     3541 gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct aaaactgttg
     3601 aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt atctttaata
     3661 tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa agcattgtat
     3721 ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg atacgtaata
     3781 aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta aaaaaggttt
     3841 tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa gaagcgtgtg
     3901 tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa aaatcactta
     3961 atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg tttattaaca
     4021 gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca
     4081 agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca agtatataca
     4141 gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg tggtgcaggg
     4201 atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt ggaaaagatg
     4261 cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc agaaacaaaa
     4321 tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga aacgctaaaa
     4381 ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca ctctgctgat
     4441 gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat cactggaggg
     4501 atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg atacttagtg
     4561 gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg
     4621 acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa
     4681 gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt tggtgccagc
     4741 aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac aaatcaagaa
     4801 ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac cgttgatatg
     4861 gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat tggtgaggtt
     4921 gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt caatgaaatg
     4981 gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc agcttatttg
     5041 atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga ccaaaaagga
     5101 tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt accaaaagaa
     5161 acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa agatgctcaa
     5221 ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa
     5281 atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta
     5341 ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt cacaggtggc
     5401 ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat ttttgggcga
     5461 caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag aacgtaaata
     5521 aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga ttgaacactg
     5581 tttagtgaag aggttgtgaa aaag
//
LOCUS       DASJST010000155         5567 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJST010000155 DASJST010000000
VERSION     DASJST010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.157"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(102..2006)
                     /locus_tag="U4B05_002533"
     CDS             complement(102..2006)
                     /locus_tag="U4B05_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2051626.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            complement(2204..2437)
                     /locus_tag="U4B05_002534"
     CDS             complement(2204..2437)
                     /locus_tag="U4B05_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEN2051627.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            complement(2584..3438)
                     /locus_tag="U4B05_002535"
     CDS             complement(2584..3438)
                     /locus_tag="U4B05_002535"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN2051628.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            complement(3423..3761)
                     /locus_tag="U4B05_002536"
     CDS             complement(3423..3761)
                     /locus_tag="U4B05_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051629.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            complement(3989..4693)
                     /locus_tag="U4B05_002537"
     CDS             complement(3989..4693)
                     /locus_tag="U4B05_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2051630.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            complement(4838..5410)
                     /locus_tag="U4B05_002538"
     CDS             complement(4838..5410)
                     /locus_tag="U4B05_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN2051631.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
BASE COUNT         1566 a         1283 c          945 g         1773 t
ORIGIN      
        1 caagaactca aaaaagcgga aaatcccaga tttaaaaatc tgagtttccg cttttgtcat
       61 ttcaaggatg gactattgtc tcagcctctt ctaatttatt cttaagatat ttttactttc
      121 tctgcaggtg ctgatacttc tttttgttct tttcgacctt tcaacttata cttcattaat
      181 ctcatgccat tgaaaattac caccaagata ctaccttcat gaaccaacat tccgatagac
      241 atattcatcc attcgctgaa gaagacgctg gtcaacaaga ccaacactac tccaattgaa
      301 atcacgatat tttgtttcat attatttgcg gtggctttta ctaatcctaa tgcgtgaggc
      361 aagttgctga agttcgagtt cattaaaacg acatcggatg tttcaatcgc tacgtccgtt
      421 ccgcttccca ttgcgattcc aatgtctgct aaagctaagg acggactatc gttaacgcca
      481 tctccaacaa aggctacaat ttgaccacgt tgttgaagtt ttccgatata agccgatttg
      541 tcttccggca acatgtagcc gtgagcttcg gtcaaaccaa gttcgccggc gaccacatca
      601 acggttcctt ggttatctcc tgaaagaacg actagatttt tcacgcccaa gtcttttaat
      661 tcctgcaaat tagctttcac acccggacgg acttgatcgc gaatgcccat cagtactttt
      721 aattcaccgt cgaccgctgt caaaacgaga gagttccctt gttgttcaaa ccgtttgaca
      781 tctttttgca cttttttgct gagagtgaca ttttcttttt ccatcaaggc cacattccca
      841 acagccactc tatgtccagc tacacttgaa acgattccgc cgcctttcac aacttcagtt
      901 ccttcaacag gataaaagtt tgtttctcca atttgattta agaccgcttt tgctaatgga
      961 tgatctgatt cccgttccac actggccaga tagccaagcg tttcagcagg atctttttca
     1021 tacagttcag tcgctgcaac ggttgggttt ccgacagtta aagtacctgt cttatcaaat
     1081 acaattgtat ccacattact gaagtcttga atgacttcac tcccttttaa aagaacaccg
     1141 ttacgagcac cattcccaat accggcaacg tttgagacag gcactccgat aactaatgcg
     1201 cctgggcaac ctagaaccaa gatggtgatt gctaactcaa tattttgact gaacgcccat
     1261 acaacaattg ccagaaccaa gacagctggt gtgtagtatt tagaaaagcg atcaatgaac
     1321 cgttccgctt cggatttaga atcttgtgct tcttccacga gttcaataat ttttccaaat
     1381 gtggtgtcct caccaactcg gtcagctctg atttgaatcg ttccgttttc taaaatggtt
     1441 cctgcaaaaa cttctgcatc agctagcttg ttgaccggaa tagcttcacc tgtaatgcta
     1501 gcttcattga tatgcccttc acccgtaagg actatgccat ctactggaac tttcgcaccc
     1561 gttttaacga gcaagatatc cccttcatct atatcatcca cttctacttc ttcaaattcg
     1621 ccattatcca tttgtttcaa ggcgctttcg ggtgccattt cagtcaattc tttgatagca
     1681 gatcgcgttt ggttcaatgt tcgttgttcc aagtagtgtc cgaataagaa taagaacgtg
     1741 acaatagccg attcttcata attttgaata aaaagcgcac caatcgctgc aatactgact
     1801 aagacatcaa tactgatgac tttgactttc attgcttgaa acgcttgaat ggcaattggc
     1861 gtaatcccaa agacagaggc gatgatcaaa gaccactctg aaagtcctac gttttctaaa
     1921 acaaagtgac tgaagaaacc gagtacgatt aacaatccac tgatgacggt gataacattt
     1981 ttcttgctta atatatattg ttgcatgctt tttccctcca aatttaattt ctcttttatt
     2041 gataaacaca gtataagata attgcctaag taattaattg actgacatca agtttagaaa
     2101 gttctctttt atcgtttcta agcacctcga atcggattgc aacaaaaaaa gcaatcctgg
     2161 aaaaatagct tttcttccag aattgcctag ttcatttaat ctgttaaacg gccttgactt
     2221 ttgctttaag aaccggatag cctacgtttt cgatagcttt ttgaatctct tcaatcgaag
     2281 tgacagctga atcaaaattg gttttgactt tgctggagtt gaataatact ttaatgctgt
     2341 ctttatcaat accgttaact gatttcacgg caccttcaat tttttgcata caacttggac
     2401 aagacaacgt ttctaattgc aatatagctt tttccataat tcctatctcc tttatgtgtt
     2461 tttattttat cggcagcaga ttaatgaaca ctgccttttc ctctttacaa ctgtagtata
     2521 gctcactttt agcaaacgaa aattgacgca tatcaagttt tgcacttctt tttatctttt
     2581 tgctcaattc accatcttaa tagtcttttt ctgcccctta ttgccatcag aataataaga
     2641 aattggtgga taatcttgtc tgcgtataaa ccatactaca atcgcgccaa taaacagggc
     2701 cagagacaag gcttgcgaaa cgcggatcca ctcgccaatc cataaactgt ccgttcgcat
     2761 accttcaatg aagaaccgac ccactgagta ccacaaaacg taactcaaag cgacttctcc
     2821 ttgacgcaaa agctgttttc ggttccgtat agtgacgatg aggacaaagc ccaacaaact
     2881 ccacaatgac tcgtataaaa aagtcggatg gtagtaggta ccattcatat tcatttgttc
     2941 aatgataaat tcaggtaaat aaagattttc caagaattga cgagtaaccg gcccaccatg
     3001 agcttcctga ttcataaaat tgccccaacg accaatggac tgggctaaca atacattggg
     3061 cgctaaaata tcaagaacga gtgccaaaga agtacctttt ttctttgcat accaatatag
     3121 cgtaccccct cctgcaatca atcccccata aatcgcaatg ccgccattcc aaatcgcaag
     3181 gatttgtcct ggattttgca gataataacc aagttcaaat aacacataat aaagccgagc
     3241 gccgaggagt ccaatcggaa ttgcccataa ggccatatcg ataatggtat cctcttccag
     3301 tccttttttc tttgcttcct tggtgctcaa ctggagagcc gctaatatgc ccgctgcaat
     3361 aatgactcca taccaatgaa tcacaacaga accaatctcc accacatggg gattcaaaac
     3421 gcctaatacg aatcccattc tatttgctcc atttcttcaa tttttcgcgt cattctagtc
     3481 atggtgatgc tgaacaagat tccggctaag ctccatggaa accaaacagt gaatacactt
     3541 ttatctccct ggtcactcgc ttcgtaatac gccacttttc cgtgtccgat tacgccggct
     3601 ccttttacaa cttcctcttc tttttccata gctaacctat ctgcctcaac aacgttagac
     3661 gtttttgctt cgccgccaat cgtgttcttg gtgaaaggga cgacaaagaa tgcccccgct
     3721 gctgacaacg tgatggcaac tatcacccaa aaacaatgca cattcaccct acttattttt
     3781 acgttttgtt cgacaaccct atcttaaaga aaaataaaac aaaaaaactt gagggttatc
     3841 atgtttgtgc ttttattttt ccaatgaatg aaggtcgttc tgtcttaaaa aagccaaaaa
     3901 cggacagatg gaagcaaaag cactgagttc tgtcccgttg ttaatgaaga gcgacagaac
     3961 accaacgtgt agaaaaaagt gtgtccgatt attcgctgta aagcaataaa tcatctaaat
     4021 cctgaatctt gatccttttc ccagacagct gttgaatcaa tccttcgtcc tccaattctc
     4081 caaatttgcg actgatggtc tcaggtgtcg ttcctaaata ggaagcaatg tccttttttg
     4141 ccatcggcag ggtgatggtg ggagagttgc ccatttcagg ttccacattt tctgccaaaa
     4201 acaaaactaa acgggtaatg acactctcca cggctacttg tgtcgtttga cgttctgaac
     4261 tttctaaccg tttagacatt tccgccaata gttttaaaga aatagctggg tattgttcca
     4321 gaaaatcttg gacatctttt tgctgaatca tacagacaac ggtatctcgg gttgcttcgg
     4381 catattcctc gtgtactgca tccgggttga acaaggtcca ttctccggtg aaatcaccag
     4441 ggttcaagat ccgcactagc tgctctttgc cagagtctgc caatcgataa atacggattt
     4501 tcccacgatt gataatatat aaagtatcct ctttttcttg tgaacgaaac agaaactctc
     4561 ctttttgata ctgtttcgtg tgggcttttt cagcaatgta attcatcgcg ctatcctcta
     4621 agtgattgaa tattggaacc aagcggatac aggctgcgtg gtcgccaatt tcatgatggg
     4681 tatgtttagt caattcattc tcccccttta tttattttgt ttatattcgc tgcttttatt
     4741 atataagtct cttactacaa aaaaattgat gcacatcaag aaaatggaaa aaggactgga
     4801 aagaattcca atccctaaat cagcttctac ttttacctta atcttcatcc tcatcaagag
     4861 cgtcatcatc ttctaatgct tctttaccaa tgaacgcttg cagcatccaa atgttcttgt
     4921 ctaagtcatt tttaaaagca atcaatgtat cttctaaagc atcgtcaccc tcgtcttgag
     4981 ctaaacgaat cgcgcgaata gttaggtcac gagtgcttct gaagtcctcg ataatatttt
     5041 caaccatgtc ttctgcttta gcgtatttat taactgcatc ttccgaaagc atcgaatatt
     5101 tttcaaattc aaccgttgtc gaagcaggtt tatgcccaga agtaattaga cgctccgcaa
     5161 gtttgtcgaa ccatttttca ttttcgttat acaactcttc aaatttttca tgcaacgtaa
     5221 agaaattggc tccttttaca taccagtgat attggtgcag tttcacatgt aatgtatgca
     5281 tgttgcctaa aagatgatcc gcaatggccg ctgcattaat tttcgtatga tgaacatgtt
     5341 ccttgtgttc ttgttcttct tgcaatcttt cttggggtgt tctttcttga gtagctttat
     5401 tttcagtcat gatttattcc tcctataatt ttggtttgtt taaaatagct ttctttgatt
     5461 cctccttttt ctttactgaa tttagtatag gatagatttc cgttaaaaaa attgacgctc
     5521 atcaagaaaa ttatttctta atagacgcca ggcttctcta ttaaatt
//
LOCUS       DASJST010000156         5505 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJST010000156 DASJST010000000
VERSION     DASJST010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.158"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            279..1127
                     /locus_tag="U4B05_002539"
     CDS             279..1127
                     /locus_tag="U4B05_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051632.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="U4B05_002540"
     CDS             complement(1298..1639)
                     /locus_tag="U4B05_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HEN2051633.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="U4B05_002541"
     CDS             1725..2432
                     /locus_tag="U4B05_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2051634.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="U4B05_002542"
     CDS             2419..2742
                     /locus_tag="U4B05_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2051635.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="U4B05_002543"
     CDS             complement(2831..3868)
                     /locus_tag="U4B05_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2051636.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="U4B05_002544"
     CDS             complement(4315..5298)
                     /locus_tag="U4B05_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN2051637.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT         1609 a         1142 c          982 g         1772 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataag
//
LOCUS       DASJST010000157         5481 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJST010000157 DASJST010000000
VERSION     DASJST010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.159"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(71..1492)
                     /locus_tag="U4B05_002545"
     CDS             complement(71..1492)
                     /locus_tag="U4B05_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2051638.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="U4B05_002546"
     CDS             complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="U4B05_002546"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2051639.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3903..5159)
                     /locus_tag="U4B05_002547"
     CDS             complement(3903..5159)
                     /locus_tag="U4B05_002547"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN2051640.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5271..5476)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1567 a         1155 c          981 g         1778 t
ORIGIN      
        1 aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag
       61 acttattttt ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct
      121 tgaagtgatt gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata
      181 atgcatcact caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga
      241 tcagcatgca gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac
      301 gttcgaattc tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca
      361 cgatacaata aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat
      421 ccattacacc ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat
      481 ttctagatac gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt
      541 cacgagctac ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa
      601 gcacaccaat aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga
      661 tttgacagtc aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta
      721 cgacatttgc tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga
      781 taaatacgcc ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac
      841 tcatttcgat cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat
      901 gttccaagtc agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta
      961 gtccgacttt gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata
     1021 cccctagcca tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga
     1081 tcgtgatgat ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa
     1141 gttctacatt accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca
     1201 tacctgggaa catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca
     1261 gtgctgtttt tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac
     1321 cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca
     1381 atgtccaacc gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg
     1441 tcagaccgat aaaggtccca agacccattt tacttacttc agatttactc accgttgaca
     1501 ctctccttga ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga
     1561 aacaattcag cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa
     1621 agacaacctc aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa
     1681 acatgcgtca gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt
     1741 tgcttcgctt gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc
     1801 tttgattttt tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac
     1861 gctggcacgc aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag
     1921 ttggttcaca aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga
     1981 agttaagaac tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc
     2041 atggttcaat ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc
     2101 taccatattg aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt
     2161 aaatgacaag tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat
     2221 taatttacca tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc
     2281 tgctgttgcc cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc
     2341 tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc
     2401 tttgatgacg ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt
     2461 tacagattct gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc
     2521 tttgttttct gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat
     2581 gaagttgttg tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac
     2641 gtgtaagtag aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc
     2701 gataccgtcg attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg
     2761 tgttttttca gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata
     2821 gttgtggtca actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc
     2881 ttttagccat gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg
     2941 ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa
     3001 cagattcatg atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac
     3061 tagttcaggt gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca
     3121 aagtccttct aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata
     3181 gctcattaat tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga
     3241 agattcatag gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga
     3301 tggcatcaat gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac
     3361 ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt
     3421 gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat
     3481 gtagttttga cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat
     3541 ttggccgttc tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa
     3601 tgattcactc atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt
     3661 acacctgttc ttctattctg taaactaagt tttggcctga tttttaatca tattttaata
     3721 tgtatcaatt atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta
     3781 caaatgtaaa ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg
     3841 ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa
     3901 ttctaatcaa ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac
     3961 ttaccatcga attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg
     4021 ttaatactta tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt
     4081 tttgtggaaa caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc
     4141 attttaccga atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac
     4201 aatgcttgcg ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta
     4261 acttcttctg ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc
     4321 gctaagtctt cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca
     4381 tcgcgatcat cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga
     4441 tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc
     4501 aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct
     4561 aatccagctg tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat
     4621 gttttatgca gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat
     4681 gagattccta cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg
     4741 aagtttttac cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag
     4801 ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt
     4861 gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg
     4921 cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc
     4981 aattggaatc ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca
     5041 gttggatcaa cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt
     5101 tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt
     5161 ttactgacct ccgtgtagtt tttttatgaa agcaggaacc tgctagcatg tgtaattaat
     5221 tatttcttca ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg
     5281 aaatctcgcg gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt
     5341 aacgctgtcg cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat
     5401 gtactggttt gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt
     5461 cgatcttcgt cttgttatta t
//
LOCUS       DASJST010000158         5312 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJST010000158 DASJST010000000
VERSION     DASJST010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.160"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B05_002548"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B05_002548"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN2051641.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="U4B05_002549"
     CDS             complement(1297..2133)
                     /locus_tag="U4B05_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051642.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="U4B05_002550"
     CDS             complement(2317..3534)
                     /locus_tag="U4B05_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2051643.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="U4B05_002551"
     CDS             complement(3683..3862)
                     /locus_tag="U4B05_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051644.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="U4B05_002552"
     tRNA            3917..4000
                     /locus_tag="U4B05_002552"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="U4B05_002553"
     CDS             complement(4240..4818)
                     /locus_tag="U4B05_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051645.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="U4B05_002554"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="U4B05_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DASJST010000159         5293 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJST010000159 DASJST010000000
VERSION     DASJST010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.161"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1375
                     /locus_tag="U4B05_002555"
     CDS             <1..1375
                     /locus_tag="U4B05_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2051646.1"
                     /translation="HLYPKNIVSKLSFELTKFGDDGADQSKQTLLKGAKFELYKGEPG
                     KGTKLGDLVSDDQGKLTATDLTLGKYYFVEVPSEVVVGSDQEPTADQYLLGADARNDA
                     HNKLTFEITNDGVTSDLKASYVNYKAPVIDKTVTNGTGQEHSFQIGDAVNYQGTIHIP
                     TDIAGGADGITVNGVKSETSPYSVFKWGDTAGQGLSYVAAKANIKVTNKDGSVVLKEN
                     TDYKIQNSENGFVIDFIVNNGQVSDTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVD
                     FVYNNNPFNQEEHHEKTKADVVTYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGK
                     YYGGLVDTDKDGVKEAVWVDDVANAAILKSDKEGHFEITGLTEGEYSLEETKAPENYQ
                     KLTKEISFKVDKDSYKEENRITIKNNQKASVPMTGSNGFQTYVLISCLLLGAGALSAV
                     VYFKKKA"
     gene            1390..2142
                     /locus_tag="U4B05_002556"
     CDS             1390..2142
                     /locus_tag="U4B05_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2051647.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            2158..2913
                     /locus_tag="U4B05_002557"
     CDS             2158..2913
                     /locus_tag="U4B05_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051648.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            2926..3186
                     /locus_tag="U4B05_002558"
     CDS             2926..3186
                     /locus_tag="U4B05_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051649.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            3183..5273
                     /locus_tag="U4B05_002559"
     CDS             3183..5273
                     /locus_tag="U4B05_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2051650.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
BASE COUNT         1947 a          871 c         1050 g         1425 t
ORIGIN      
        1 tcatttgtat cctaagaaca ttgtttctaa gttgagtttt gaattaacga aatttggtga
       61 cgatggcgca gaccaaagca aacaaaccct tttaaaaggg gcaaaatttg agttatacaa
      121 gggcgaacct ggaaaaggaa caaaattggg tgatcttgta tcggatgatc aaggaaagct
      181 gactgcgact gatttgacgt taggaaaata ctattttgtg gaagttcctt cggaagttgt
      241 agtgggatca gatcaagaac caacagcaga tcaatattta ttaggcgcag acgctcgcaa
      301 cgatgcgcac aataaattaa cctttgaaat tacgaatgat ggcgtgacaa gtgacttaaa
      361 agcttcatac gtaaactata aagcaccagt gatcgataaa acagtaacaa atggaacagg
      421 acaagaacat tctttccaaa ttggtgacgc agtaaattat caaggaacga ttcatattcc
      481 aacagacatt gcaggtggcg cagacggtat cacagtaaac ggtgtgaaaa gtgaaacttc
      541 tccttacagt gtatttaaat ggggagatac agcaggacag ggactttctt atgtcgctgc
      601 taaagcaaat atcaaagtaa caaataaaga tggatctgtg gtattaaaag aaaatacaga
      661 ttataaaatc caaaattcag aaaatggctt tgtgatcgat tttatcgtga ataatggcca
      721 agtaagtgat actgtagcaa gtttacacgg acaagatctt caaatgactt ataacatgta
      781 tgtaaatgac agcgcagctg ttgccaatcc attaacaaat agcgtggatt tcgtttataa
      841 caacaatcca tttaatcagg aagaacacca cgaaaaaacg aaagctgacg ttgtgactta
      901 tggtgcaaaa ttcttaaaag tcgatagcgg actatttgga acaggaatca aagcaacacc
      961 gcttgaaggc gcagaatttg ctgctaaaaa cgcagaagga aaatattatg gtggattagt
     1021 tgatacggat aaagacggtg tcaaagaagc agtatgggta gacgatgtag caaatgcagc
     1081 gatcctaaaa tcagataaag aaggtcactt tgaaatcact ggtttaactg aaggtgaata
     1141 ttcactggaa gaaacaaaag cgcctgaaaa ctatcaaaaa ttaacgaaag agatctcttt
     1201 caaagtagac aaagattcat acaaagaaga aaaccgaatc acgatcaaaa acaatcaaaa
     1261 agcttccgta ccaatgaccg gtagcaacgg ttttcaaacg tatgtattaa tctcttgctt
     1321 gttgctagga gcaggggcac tttctgcagt tgtttacttt aagaagaaag cctagaagga
     1381 tcaaaaccaa tgattatcag gaagcctaag aaaaagcgaa tcatgggtaa atggatcatt
     1441 gctttctggt tactttcagc tgtaggtgtc ttacttttga tgcctgctga agcgagcgtg
     1501 gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac ggcagcagaa
     1561 acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg tattcttaca
     1621 attctaagta tttctttaaa actaccaatt tatgacggaa cgtccgacaa aattttagaa
     1681 aatggtgtag gtattacgga aggaactggt gatattacag gaggaaacgg taaaaatcct
     1741 ttaatcgcag gtcatagtgg actttataaa gataatcttt ttgatgattt accttcagta
     1801 aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta tcaaatcgat
     1861 cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta cttagaatca
     1921 aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat caacacacac
     1981 cgttttctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa agatgaagaa
     2041 aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt tctttcagtc
     2101 atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt aaccaacatg
     2161 agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac tgcctacaca
     2221 cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac aatccaaacg
     2281 tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac agttgattct
     2341 tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat tcacgttcaa
     2401 aagattattt cttcagaaca attagacaac caggggcatg aaaaacaaga aggagtaaat
     2461 ggcgcgaagt tttctgttta tgatgtatcg gacattttac aaaagatgga tgttaaggat
     2521 ctcactactg accaaattga atcccaatta aaaaatcgag tgaaaaaact atccagtgat
     2581 caattgaagc tagtttctaa tgaagaaaca aagacaattg atcaacaaac aggtgtcttt
     2641 gaattctcgg tagaagtaca ggcaaatcag aaacaagctt attatattgt caatgaatca
     2701 agtcctgaaa acatttctaa tagcgaggat attttgctat taacacctgt ttctgataaa
     2761 aacggtgaat tcttaaagga tgtgtggatc tatcctaaat cagaagcttc gcaacctaaa
     2821 gaagaagtaa aaaaaatcgt ttctactggc gtaaagaaaa acttctttga gaattgttgg
     2881 gattttgtta ctcacttgtt ttctagagat taaggaagaa ataaaatgaa actgatcaaa
     2941 ttattattat tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg tttattattt
     3001 tctaaactat ctattttgcc aagctaccaa ctatacctaa tgaggatttt aaacatacca
     3061 gctgcttcca tgccgctaga tattatggaa aagctcatta ggaatccatt atttactacg
     3121 atcaatttga tcgttattgt cggaatttca ctagtcattt ataaaaaaga ggggatgaag
     3181 ggatgaattt taaacactcg tggacaagac ggttaaaata tgtttttccc ataatgatga
     3241 tgttaggtac attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa gttattgtcg
     3301 atccgtcaaa tccaatccag aacgtaaaga ataattttat tattccaaaa gatgttccta
     3361 atgcaaaaac aaatattact gtaaacggag cgtcaaaagt ttacaaatat catgttgatg
     3421 ccaacaaagg tggttttaca gacgactacg taggattagt ggagggaaac aaaaatatac
     3481 gctatccttc tttccaaaaa gaatacggtg aaacaaacct tgttttagaa ggcgtaacaa
     3541 ccaacaccaa agtcaatatt gactatggaa aaatcggtac gtataacggg aaaaaagtca
     3601 atatcaagct agttctatct aatattcact tatactcgga tacgttgccg tggaatctac
     3661 tagataacaa ctatacaaaa actcattttc gagacgatgg ctataaaaat acgaatggtg
     3721 caatgagtaa atctaaaaaa agaacagtac tttggatctc agacaatctt ttctcaggaa
     3781 ttgtttacca ttcaacacaa atgaatgtgc agctagtagc cacatatgag gacggtagcc
     3841 ccgttcaatt ttcaggagat acatttatca gttttaactc tttgaaccct gcaggaggaa
     3901 aaagcacgga tcttaaagga gaatatgccc actatgacaa aatgaatacg acagattggt
     3961 atgtcagaaa agatacggtt ctttcagaat ttaaatcttt ctacaacaat ctgaacgtgg
     4021 taggtggcca cccaggagga agctctaagc tgacacaagc ggataatgat ttcaataatt
     4081 tgcatgataa attaggagat ccgaagtttg gtcaaggtac tgtgtccttt aaaatatctg
     4141 aagcaaatcc gacgtttgtg attggttctt ctaatgttca aacctggttt actttatctt
     4201 ctgcgacaat ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt gataaaaatg
     4261 gaaataacgt gaacgataaa atgttgcaag taggagacac gattcaatat cgaatcaaac
     4321 agaaggtgaa tcgattagga gtggatttac tagcggttta cgatcgattt gagttaatcg
     4381 ataatttacc aaaagaagta aattacgtcg atgcacacgt tgaaagtggt acaaataaaa
     4441 aattcgatgt atctggtgaa gtgacctatg acaaaacaaa acaccaggta aaatatgcag
     4501 cgaaagctga tacattaaaa aatcgaatga agtataacgg cgaaacttac gaattagtga
     4561 tcaatgtaaa agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat cagggaacgt
     4621 cgatcattaa taaagtagaa aaggatacaa atatcattac cgtttatttc cctaaaattc
     4681 cagtgaaaga agtacaacaa aatggaaaag atgtaaacgg acgaaatgac ggtaagaagg
     4741 gtacgccgac agctccttta aacgcaggat ctgaagtaca atatctagtc acacaaaaat
     4801 ggcacacaaa aggcgtagac gctgcgtcag atcactacaa acaatttagt attcaagatc
     4861 caattgaagc tcgtttgaca tataaagaag gttctgcgca agtaatagat aagagtaaag
     4921 gaaaagatat tacttctgaa ggcacactaa cctatgatag caattcaaga acgctgaagt
     4981 gggaagcttc cgctgacttc ttaagtaaca atcttttaga tggacgagaa attcagctga
     5041 tatttacagc taaaactcca ttacaatcag aaaaaaatat tgataaccaa gccgtagtag
     5101 cagtagataa tgtttctaat aaaacaaatg ttgtgacaat tggggttgat cctaacttgc
     5161 cacaggtcat tgttcctaaa acaggttcta cacatttagt aacgatttca gcggtcagct
     5221 tactattact tgtgttagca acttttagtt atgcggttct gagatacatt taaaataaaa
     5281 aaacgaaaga atg
//
LOCUS       DASJST010000160         5289 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.162, whole genome shotgun sequence.
ACCESSION   DASJST010000160 DASJST010000000
VERSION     DASJST010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.162"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..983
                     /locus_tag="U4B05_002560"
     CDS             51..983
                     /locus_tag="U4B05_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051651.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1204..2967
                     /locus_tag="U4B05_002561"
     CDS             1204..2967
                     /locus_tag="U4B05_002561"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN2051652.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3051..3509
                     /locus_tag="U4B05_002562"
     CDS             3051..3509
                     /locus_tag="U4B05_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN2051653.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3618..4301
                     /locus_tag="U4B05_002563"
     CDS             3618..4301
                     /locus_tag="U4B05_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2051654.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4448..5062
                     /locus_tag="U4B05_002564"
     CDS             4448..5062
                     /locus_tag="U4B05_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN2051655.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5078..5284
                     /locus_tag="U4B05_002565"
     CDS             5078..5284
                     /locus_tag="U4B05_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051656.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1882 a          832 c         1092 g         1483 t
ORIGIN      
        1 gggaaggcta tgaaaataaa aatccgtatg tagaagaagc ttgtaacgag ttgattgatt
       61 atgtagagac tcattttttt aaaagaagat attcggaaga tgaaaagtcc tttatcatac
      121 gaggaataga gatcgcaatc ggaagaaagg catttcccct tggactgaaa gaagaagata
      181 agaaaagcgc agaaacgaag ccgttatttc aattagtctg tcaaggattg gaagagcaaa
      241 aaggaattct agacttacaa gaagaagatg tctattatat tttttctttg ttcaacagta
      301 gaaattatac caatgagaac atggagctgt taagaaaaga tttcgaagtt gtttataaaa
      361 attttgttct caatgaccta tcactgaatg aattgatttt acagattatt agtatcgctg
      421 aaatcgacga tgcagaaaat tttattttca agcaatcctt tttacaattt gtccgtacgt
      481 tgtgggcaga tggacaagta tttttaccgg aaaggatcta tcttctatca gaaaaagaac
      541 aacttctgta cggaaaaatt gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc
      601 aactaagatg gaatgaaaat ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca
      661 gtgaacaagc ggggagacag gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac
      721 aattttttta tcgaacccaa tttcatttgt atgcggaaga aggcatcgct gaaataaatc
      781 ctattttatt tcgttcatta gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag
      841 tggttttgtg cgatgctgct agctatcaaa atggattaga aactgaaaag acaaagatct
      901 atccaatctc attaaaaggt gcaaacatcc atttgtatca tattgctcag gaacttcacc
      961 tattaaaaaa acatatttct tgatgagacc ttaattagga gaccttctac aaagcttttt
     1021 aattgtctgt agaaggtttt tatttgagtc ctgattcgtg cgaagatcta cttatataac
     1081 aagctttctc tgaaattttt ttaaaatgct attgacttag agtcaactct aagtagtaga
     1141 atagataaag tgtaacaaat ctattttcat taaaagaaga tagtacggag gagagaatgt
     1201 attatgagta cagtattaca tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa
     1261 ttatctgttg aggaaatgaa acagttggca gaagagatgc gccaaaaaat cttgcaaaag
     1321 gacagcgatg taggtgggca cgttggtccg aatctaggtg tagtagaatt aacgattgcg
     1381 tttcattatg tatttgattc accaaaggat aaaatcgttt gggatgtttc ccatcagtct
     1441 tatggtcata agcttttaac tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa
     1501 gtgagcggat attccaacca acatgaaagt aaacatgact attttacagt ggggcacaca
     1561 tctacatcta tcagcttagc aacaggttta gctaaagcga gagatctgaa gaaagaatca
     1621 ggaaatgtgg tagcatttat cggtgacggc tccctaagtg gtggcttagc ttttgaagga
     1681 ttgaacaatg gtggtgtttt acaatccaat ctaatcgtat tagtaaacga taatgagatg
     1741 tctattgatg agaatcatgg cagtttatat aaaaacttag ctgagcttcg tcaaaccaac
     1801 ggtaaaagca gcaataacct atttaaagca atgggatttg attatcgtta cgtggataat
     1861 ggaaacgatc tggaaacggt gatccgttta tttgaagaag taaaagatat tgatcatccg
     1921 attgtacttc acgtccatac ggaaaaaggt cgcggttatc agccggcaat cgataataaa
     1981 atgaaatatc attggcatgt accatttgat ctgtccaccg gggaggcaaa gcaagtaact
     2041 tctggagaaa catttaatat gatcatcatg gattatttgg ataagaaaat gcaaacaagt
     2101 acagctccta ttgccgcagt caatgcagcg ataccaggcg cctttggact gaaaacattc
     2161 caagaaaaac atccagatca ttattttgac gtggggatcg ctgaacagca ctctatcact
     2221 ttcgcttctg ctttggcttc tcagggagta cgtccagtcg tcttccaatc aagtactttc
     2281 ttgcaacggg catatgatca attatcacat gatttagcga tcaacgagaa tccagtgatt
     2341 attttagtcg gtggcggaac gatctcagct agtgaccgaa cacaccaagg aacatttgac
     2401 attccttact tatcaagtat cccgaacatc aaatatcttg ctccagcaac aaaagaggat
     2461 atgatctcta tgctagattg ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt
     2521 cctacacatg gtgtagaaca tggtacagct atcgcaaatg attatagtat tccagcctat
     2581 caaacagtaa ataatggagg aaaagtagca attttagctt taggcggttt tcttgaatta
     2641 ggaaaagaaa cagtaaaagc ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca
     2701 ggaacgatca cagaaaatga cgtagaaaca ttagataaat taaaagaaac acacgaatta
     2761 gtcatcacat tagaagatgg tagtttgtca ggaggatttg gcgaaaatat cagtcgttat
     2821 tatggtgatt cttcaatgaa cgtattaaat tttggtgcag agaaagaatt cacggatagc
     2881 gttcctgtag ggcagcttta tgaacgttat catttaacac caaaacaaat cgttgaagat
     2941 agtctgcgtg tcttgaatat tcaataattc attatgctca ccatccagat aattgtttat
     3001 actagtatct ggatggtttt ttcattataa aaagcaaagg ggtggatgtt atgtatcagc
     3061 aagtagaaga atatgtcaaa aagttccaag aaaaatatcc tagttatcaa tcagaaaaat
     3121 taggtttctt ttcatttggg gatacagaaa aaatggcaga cgagttgagc gaactagttg
     3181 taaaaaagat aaagactggt acatcaagtg gttacgatct gtataaacaa gagaatgagc
     3241 ctttaccaaa agttggtcaa ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta
     3301 tcatcaaaaa tataaatgtg accatcttgc cttttagtga agtcacgaaa gagcatgctt
     3361 ggaaagaagg agaaggggat cggacattag cttattggcg gaaagtccat gctgagtttt
     3421 ttaaacatgc ttataaagaa gcgttgaaca tagaatttac agaagagaag ctggtcgtat
     3481 acgaagaatt tgaagtaatt ttcttttagt aagaaaggtc tgctgaaaga aatagctcat
     3541 tattgtctcc tgaatagaaa agggttacat ttttatatga agctgtattt ttatataaca
     3601 ttactggagg agatattgtg gatacactgg aacaaatcaa aggcggagtc gtcgtttctt
     3661 gtcaagcttt ggaaaacgag ccgctacaca gttcttttat tatggggaga atggctttgg
     3721 ctgcaaaaga aggcggagca gttggtattc gagcaaatac gacagaagat atcaatgaaa
     3781 tcaagcgaat cgttgattta ccggtaatag ggattatcaa aagagattac gatgggtcag
     3841 aagtgtttat tactgcgaca gaaaaagaag tggaagaact tttggagacg caagcagaaa
     3901 tcattgcact ggatgcaact tctcgtaaac gtccgacaga aaaaacaact gctgaacttg
     3961 tccaaatgat tcacgaacac ggtcggttag ctatggcaga tatttcgact tttgaggaag
     4021 cgcttcaagc gcaagaagat ggttttgatc ttttgtctac gactttagca ggctatacag
     4081 aatattcacg aaagacgcag acacctgatt ttgatttatt gaatcaaatc atagaacaag
     4141 tcgatatccc agtaattatg gaaggacata ctgactctcc tgaacaagta gagaaagcat
     4201 atgaattagg tgctttttca gtcgtagttg gatctattat cacccgtccg caattgatca
     4261 ctaaaagata tgtcgaagcc gcaagtaaag caaataacta aaaaacacgc acggcacaga
     4321 ttcttgacaa aaaagaaaaa ttgatagata gtttaaagca gattctaaga agatgaaaaa
     4381 agcattactt ttcattttgt cttcaagatc tgctttttaa cgaattttat agaaagaagg
     4441 cgaaaccatg tttgaacgga gtccttcaga attgacagaa cgtgaaaatt acaagttgct
     4501 gattggttca gtcatcccta gaccagtagc agtagtaacg actaaatcag caaaagatat
     4561 agtgaatatc gcacccttca gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc
     4621 cttggcgatc cagagaaaac aagatgcagt aaaagacaca gcaaagaata ttcttgagac
     4681 aagagaagct gttatccata tccttgatga ggacaacgta gaagaagtaa ataaaacagc
     4741 agccaatctg cctaatgagg aaagcgaact gtacctgact gacttgactt taactgattc
     4801 agtaatcatt tcagtaccag cattgacaga agcaaaagtt cgttttgaaa catccttata
     4861 tgaacatgtc gagatacgga ataaaaataa agtaacagct gatttatttt tgctagaagt
     4921 acagaaatat catttaaatg aaacagtcta tgatgataag acaggaagga tcgatccaag
     4981 aaaattgaat gctgtaagcc gtttggcagg aaatgattat gcagcaatag gagaaatttt
     5041 cacgattgca cgtcccgaat aaaaaggagc actagttatg ttaaaaataa gctataaacc
     5101 atcgacagat tcaaaagaaa tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg
     5161 gcagtactta gaagttccgc cattgcaaga ccattttgtc gttactactg ttactttaga
     5221 tggtaaggaa atagaaatgc cagatcaaac gattagcgga ttgtttaatt actttaacaa
     5281 ataaatatt
//
LOCUS       DASJST010000161         5208 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJST010000161 DASJST010000000
VERSION     DASJST010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.163"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1467
                     /gene="ftsH"
                     /locus_tag="U4B05_002566"
     CDS             <1..1467
                     /gene="ftsH"
                     /locus_tag="U4B05_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN2051657.1"
                     /translation="LGARIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVE
                     MFVGVGASRVRDLFETAKKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVE
                     MDGFDGNEGVIVIAATNRSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKP
                     MADDVDLKVVAQQTPGFAGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPA
                     KKDRVISKKEREMVAYHEAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQF
                     LMTKEDMFEQIVGLLGGRTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGP
                     VQYEGNHQVFVGRDYGQTKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLI
                     AEKLLEFETLDAKAIKSLFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQA
                     EEKRDFEEAKHELNEENKAEHDSDSQQSEDNHSDDQYK"
     gene            1684..2571
                     /gene="hslO"
                     /locus_tag="U4B05_002567"
     CDS             1684..2571
                     /gene="hslO"
                     /locus_tag="U4B05_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN2051658.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            2572..3576
                     /gene="dusB"
                     /locus_tag="U4B05_002568"
     CDS             2572..3576
                     /gene="dusB"
                     /locus_tag="U4B05_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN2051659.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            3687..5183
                     /gene="lysS"
                     /locus_tag="U4B05_002569"
     CDS             3687..5183
                     /gene="lysS"
                     /locus_tag="U4B05_002569"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN2051660.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT         1797 a          925 c         1207 g         1279 t
ORIGIN      
        1 ttaggtgccc gtatcccggc aggtgtactt ttagaaggac ctccaggaac aggtaaaacc
       61 ttattagcaa aagctgttgc cggagaagct ggagtaccgt tttactcgat ctcgggttct
      121 gatttcgtag aaatgtttgt cggtgttgga gcaagtcgtg ttcgtgattt gtttgaaaca
      181 gcgaagaaaa acgcaccagc tatcattttc attgatgaaa ttgatgcggt aggtcgtcaa
      241 cgtggtgccg gtatgggcgg cggacatgat gaacgcgaac aaacattgaa ccaattgctt
      301 gtagaaatgg atggcttcga cggaaatgaa ggagtaatcg tcattgctgc aaccaaccgt
      361 tctgatgtac tagacccagc gttactacgt ccaggccgtt ttgaccgcca gattttagtt
      421 ggacgtccag atgtaaaagg tcgtgaagca atcttgcgag tccatgcacg gaataaaccg
      481 atggcagatg acgttgactt gaaagttgtg gctcaacaga caccaggttt tgccggagca
      541 gatttagaaa atgtcttgaa tgaagcagcg ttagtagctg ctagaagaaa taaaaagaaa
      601 atcgatgctt ctgatatcga tgaagcggaa gatcgcgtga ttgcaggtcc agctaaaaaa
      661 gatcgcgtaa tcagcaaaaa agaacgcgaa atggttgctt atcacgaagc tggtcatacg
      721 attgtcggat tagtattgag tcgcgctcgg gtcgtccaca aagtaacgat catccctcgc
      781 ggacgcgccg gcggatatat gatcgcttta ccaaaagaag atcagttctt gatgacgaaa
      841 gaagatatgt ttgaacaaat cgtaggctta cttggtggac gtacagctga agaaatcatc
      901 ttcaatgtcc aatcaactgg tgcttccaat gactttgagc aagcgacagc tctagcacga
      961 agcatggtaa cagaatatgg aatgagtgat cgtttaggtc ctgttcaata tgaaggaaac
     1021 catcaagtgt ttgttggccg cgactatggt caaacaaaag cttactcgga acaagtcgct
     1081 ttcgagattg atcaagaagt acgaaaaatc ttgatggaag ctcatgataa agctcgagaa
     1141 atcatcgaag ctcatcgtgc acagcataaa ctgattgctg aaaaactttt ggaattcgaa
     1201 acattggatg ccaaagcaat caagtcattg tttgaacacg gtgtgatgcc tgaaggtact
     1261 gagtcaaatg attatccaag cgaaaaagaa gcgaaaactt ttgaagaagc aaaacgtgct
     1321 ttggaagaaa aagacgctga aaaacaagcg gaagaaaaac gtgattttga agaagcaaaa
     1381 catgaactga atgaggaaaa taaagcagaa catgattcag attctcaaca atcagaagat
     1441 aatcattcag acgatcaata caaataaaaa aatataggtc aattgatctg atatgactcc
     1501 cgaaaaccgg aacaaaaacc ggttttcggg agatttcttt atttaagttg taaatagggt
     1561 gatgtcctgt tttctagatg attctgaatt atttttaaaa gaagcattcc aaagttgaaa
     1621 aaagcagtag ttgctagtat gattcactat gagtttaaaa acggaaaaag gagtacataa
     1681 caaatgaaag attatttggt taaggcactt gctcacgacg gattcgtccg agcatacgcc
     1741 gttcaagcaa caaataccgt agcggaggca caaagacgtc atgatacttg gcacacctcc
     1801 tcagctgctt tgggacggac attggtcggt agtctgctac ttggtgcgac gctcaaagga
     1861 gaagacaaac taactgttcg aatccaaggc gatggtccag caacaggaat tgtagtagat
     1921 gcaaatggaa agggagatgt gaaaggttac atcaaaaatc cgcatctcag tcttcctcta
     1981 aatagtgaag gtaaaatcga tgtgcgggga gcagttggaa cacaaggaat gtttactgtc
     2041 acaaaagact taggattaaa agaacctttt tctgggcaaa cgccgattgt ttctggagaa
     2101 atcgctgaag attttactta ctatcttgct atctcagaac aaatcccttc agctgttgga
     2161 ctgagtgtat tggtcaatac tgacgacagc atcaagacgg cgggaggatt catgatccaa
     2221 atcatgccag gtgccgatga agaaacaatc agcaagatcg aagaacaatt aaaaacgaca
     2281 ccaagaatct ctaccctctt agatgaagga cagacaccag aagaaatatt gaaaggattg
     2341 cttggtacag aagaattgaa cattttggaa aaaatgcctg ttcaattcaa atgtgactgt
     2401 tcaaaagaaa aatttgcaga agcaattatc gcgttaggtg cagaagaaat ccaagcaatg
     2461 atagacgaag atcacggtgc agaagcggtt tgttctttct gtaataacaa atacaagtat
     2521 agcgaagaag aattagatgg attgaagaaa gaagccttag gagggattta agtggaacac
     2581 acatggaaaa ttggcaatgt ggaaatccct aatcgtgtcg ttgttgcacc gatggctgga
     2641 ataacaaatt cagcatttcg agtcactgtc aaagaatttg gtgcgggctt agtcgtatgt
     2701 gaaatgatca gtgaccaagg tattcatttc agaaacaaaa aaactttaga gatgctccac
     2761 attgaagaaa cagaacatcc attaagttta cagatatttg gcggaaataa agatagtcta
     2821 gttgaagctg ctcaatttgt cgaagaaaac acagctgccg atataataga tatcaatatg
     2881 ggctgtccag taaataaagt aatcaaagca gaagcaggag caaaatggct tttagatcct
     2941 gagaaagtat acgaaatggt ccaagctgtt tcttcagcag taaatatccc agtaacaatc
     3001 aagatgcgta ttggttggga tgaagagcac gtctttgctg tagaaaatgc attagcagca
     3061 caatcagctg gagcaagtgc catcgctatg catggacgta cacgggttca gatgtatgaa
     3121 ggaaaagcgg actgggaagt attgaaagaa gtgaaaaaac atttgacgat cccatttatg
     3181 gggaacgggg atgtcaaaac gccagaagat gcaaaatgga tgcttgacta tgtcggtgca
     3241 gacggtgtga tgattggtcg tgcagcgctg ggcaatccat ggatgattca ccgcacacag
     3301 cattatctcg aaacaggaga actgattcct gaaccaacac cagccgaaaa aattgctaca
     3361 gctaaacttc atttacaacg cttagcagat ctcaaaggag aaaaagtaga ttgtagagaa
     3421 ttcagaacac atgcggctta ttatttaaaa gggattcctc gagcagcgaa agtgaaagta
     3481 gcaatcaatc agacagaaaa acagcaagaa atcaatgacc tgctggatca atttgtagaa
     3541 aaagcggaaa aagtactatt gaaacaaacg ttgtgatttc taagtttttc gtgaattttc
     3601 aagccgaaat tccaagttcg gcttgctttt tttgtgtttc attggcatta taggaaagag
     3661 attaacatga gaatggagga acttttgtga cacaggaaca acaacatcaa gtagaagatt
     3721 taaatgatca aatgcttgta cgtcgtcaaa aaatggaaca attgcgtgaa gatggaatcg
     3781 atccatttgg aaaacgattt gaacgcacgc acaactcaga agaattgcat gaactatttg
     3841 atccaagaac aaaagaagaa ctagcagaaa tgggattgac cgctagtgta gctggtcgta
     3901 tgatgactcg ccgaggaaaa gggaaaactg gatttgcgca tctacaagat cgtgaaggcc
     3961 aaattcaaat ttatgtaaga aaagaccaag taggtgatga ggcctatgaa gtattcaaac
     4021 atgcagacct tggagatttc tttggtgtaa ctggacaaat catgaaaaca gacacaggcg
     4081 aagtttctat caaagcttca gaaatcacga ttttgtcaaa agcacttcgt ccactgccag
     4141 ataaatacca tggcttaacg aatattgaac aacgctacag acaacgctac ttggatttaa
     4201 tcagtaatcg cgaaagcttt gaccgattta tgaaacgaag ccaaattatc agcgaaatcc
     4261 gtcgctattt agatggcaat ggctatattg aagtagaaac accagtattg cacaatgagg
     4321 caggtggagc tgccgcacgc ccatttatca cgcatcataa tgcactggac attgatttat
     4381 atttgcgtat tgcgctagaa cttcatttaa aacgactaat tgttggcgga atggaaaaag
     4441 tttatgaaat cggccgcgtt ttccgtaatg aaggaattga tactacacat aatccagaat
     4501 ttacgatgtt agaagcatac acagcatata ctgatttcaa tgatgtaatg aatctgacag
     4561 aaggtattat ccgtaacgca gctgaaaaag tactaggaac agcaaaaatc acatatgatg
     4621 gacaagctgt cgacttagaa agtgacttta aacgcatcca tatggtagat gcaatcaaag
     4681 aacaaactgg tgtagacttc tggcaagaaa tgacattgga agaagcatta gctttagccg
     4741 aaaaacacaa tgtagaagta accgaagcta tgggtgtcgg acatgttatc aatgaattct
     4801 ttgaaacatt tgttgaagaa acattgacac aaccgacgtt tgtatacgga catccagtcg
     4861 aagtttcgcc tttagcgaag aaaaatccag aagatcctcg attcacagat cgattcgaac
     4921 tattcatagt ggggcgcgaa tttgctaatg cctttacaga actgaacgat ccaattgatc
     4981 aaagagaaag attcgaagcg caagaaaaag aacgtgaact aggaaatgac gaagctcacg
     5041 gtgtagacga agacttcttg gaagccttag aatacggtat gccgccaact ggtggtctag
     5101 gcatcgggat cgacagatta gtaatgttgc taactgatgc acaatcaatt cgggatgttt
     5161 tgctattccc aacaatgaga taatcgaata aaagctgtct aaggaatt
//
LOCUS       DASJST010000162         5207 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJST010000162 DASJST010000000
VERSION     DASJST010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.164"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..1368
                     /locus_tag="U4B05_002570"
     CDS             34..1368
                     /locus_tag="U4B05_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051661.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..2989
                     /locus_tag="U4B05_002571"
     CDS             1355..2989
                     /locus_tag="U4B05_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051662.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="U4B05_002572"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="U4B05_002572"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2051663.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4110..5066
                     /locus_tag="U4B05_002573"
     CDS             4110..5066
                     /locus_tag="U4B05_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2051664.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DASJST010000163         5207 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJST010000163 DASJST010000000
VERSION     DASJST010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.165"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..1040)
                     /locus_tag="U4B05_002574"
     CDS             complement(24..1040)
                     /locus_tag="U4B05_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2051665.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            1260..2348
                     /locus_tag="U4B05_002575"
     CDS             1260..2348
                     /locus_tag="U4B05_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN2051666.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            2402..3928
                     /locus_tag="U4B05_002576"
     CDS             2402..3928
                     /locus_tag="U4B05_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN2051667.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            3930..5135
                     /locus_tag="U4B05_002577"
     CDS             3930..5135
                     /locus_tag="U4B05_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2051668.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         1636 a          888 c         1206 g         1477 t
ORIGIN      
        1 tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc
       61 tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata
      121 tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg
      181 actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata
      241 gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca
      301 ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt
      361 tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg
      421 tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta
      481 cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa
      541 ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca
      601 aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc
      661 tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt
      721 gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc
      781 actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca
      841 tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc
      901 tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt
      961 gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa
     1021 aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc
     1081 agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa
     1141 aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca
     1201 atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa
     1261 tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat
     1321 taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta
     1381 tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag
     1441 aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag
     1501 cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg
     1561 gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct
     1621 atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg
     1681 gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag
     1741 gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt
     1801 acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat
     1861 ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag
     1921 caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt
     1981 cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga
     2041 gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat
     2101 cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa
     2161 atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg
     2221 aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg
     2281 ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga
     2341 aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat
     2401 tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc
     2461 gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa
     2521 cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta
     2581 tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga
     2641 agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa
     2701 agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac
     2761 agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct
     2821 agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa
     2881 atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc
     2941 gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc
     3001 acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca
     3061 gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt
     3121 gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt
     3181 taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca
     3241 catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg
     3301 cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg
     3361 tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg
     3421 actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg
     3481 ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga
     3541 aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat
     3601 ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg
     3661 gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg
     3721 ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg
     3781 tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa
     3841 cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa
     3901 attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa
     3961 aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg
     4021 tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc
     4081 gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag
     4141 ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt
     4201 ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag
     4261 ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt
     4321 atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga
     4381 cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt
     4441 ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt
     4501 taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga
     4561 aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag
     4621 cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt
     4681 tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag
     4741 gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa
     4801 caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat
     4861 tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg
     4921 atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca
     4981 tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag
     5041 gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc
     5101 tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa
     5161 gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaa
//
LOCUS       DASJST010000164         5157 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJST010000164 DASJST010000000
VERSION     DASJST010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.166"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..223)
                     /locus_tag="U4B05_002578"
     CDS             complement(<1..223)
                     /locus_tag="U4B05_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN2051669.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLI"
     gene            381..644
                     /locus_tag="U4B05_002579"
     CDS             381..644
                     /locus_tag="U4B05_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051670.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            668..856
                     /locus_tag="U4B05_002580"
     CDS             668..856
                     /locus_tag="U4B05_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051671.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(939..1124)
                     /locus_tag="U4B05_002581"
     CDS             complement(939..1124)
                     /locus_tag="U4B05_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051672.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1339..1710
                     /locus_tag="U4B05_002582"
     CDS             1339..1710
                     /locus_tag="U4B05_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2051673.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1860..1932)
                     /locus_tag="U4B05_002583"
     tRNA            complement(1860..1932)
                     /locus_tag="U4B05_002583"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1897..1899),aa:Gln,seq:ctg)
     gene            complement(1978..2406)
                     /locus_tag="U4B05_002584"
     CDS             complement(1978..2406)
                     /locus_tag="U4B05_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051674.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2466..3224)
                     /locus_tag="U4B05_002585"
     CDS             complement(2466..3224)
                     /locus_tag="U4B05_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2051675.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3519..3716
                     /locus_tag="U4B05_002586"
     CDS             3519..3716
                     /locus_tag="U4B05_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051676.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3822..4304
                     /locus_tag="U4B05_002587"
     CDS             3822..4304
                     /locus_tag="U4B05_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051677.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4362..4958)
                     /locus_tag="U4B05_002588"
     CDS             complement(4362..4958)
                     /locus_tag="U4B05_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2051678.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1675 a          799 c          991 g         1692 t
ORIGIN      
        1 taataagaaa aattactgaa agcgtcaatt cgatgatgtg tgttattaat acagacgttg
       61 tttcccaatg cctaaaagaa aaaatttgat aagtaaaata tacagcaggt aaagaggcca
      121 gtaaaactga aaacttcttt tttgtccatt ttatgtaata gaaaagaatg cttatcataa
      181 taaaaaaagg aatataaaaa acaaattgac cgatcgcgat catactattg cctccgataa
      241 ataaagtcca tgatacaaaa taacactttg tgcgaaatgg cgcaagcagt tttgtggatt
      301 tatccggtaa aatctgtatc gattttgaaa ttagaggaga aacttgctat aatcaaagtg
      361 aaagaaggag tgggattttc atggatcatg tagtgttaga aatcgatttg catttgatca
      421 ataatattcg ggtgatttac tatgttgtaa gtaacagtgt cgaacaacgt gtactgacga
      481 ataaaataaa tggtatttta gcaaagaaag acgttcaccg attcaataat ggtgaaggaa
      541 gttattatag tatcccagta gaaaaaatta tttatacgac agtcaaagtc agagaagatc
      601 tagataccaa aaaagcatat gaacctattt ttacaactta ttgattgaca gcagatggag
      661 gaaaaaaatg aatccatatg aacaacttat tcaagatttg atcgatggaa aaatcgaaaa
      721 gattgatgta aaaagagaag aattaatggc ttttcgagaa gcgtggctga agttggagaa
      781 tcgtaaatat gtggtgggaa aagcctcact gaacggcaat gtgacttatc attacgatcc
      841 gacacgttta ttttagtatg aagtattcag aaaagaacca aaggaggacg atgcagcagc
      901 attgtcctcc tttgatttta caaaagtgaa agttttactt atttgaatag atttttaaat
      961 ttgtttttga cactgtcgat atttacatct tttaaaagat tttgggcttt ctctttataa
     1021 ggtcctaatt gttctttatg ttcttcgata aatcgttttg cgccatcgaa attgccgttc
     1081 ttaatcattt ccttgacttt ttgaaataat tcttcttgat tcattttgaa cacatccttt
     1141 gctatcaaca tgatttttta catggacaaa acgagaaaaa ctgatttatc cacacgtcaa
     1201 taatatcaaa taacgatcat ttttactaaa aaaacactcg attctcgctt ctaaagtagg
     1261 agaaaaatct ccaacttggg acggatggaa tttctttaac tgacccatat actgaatata
     1321 taacaaggag gtagaaagat gaaagtgatt aaatatttga ttggactggc ggcgattagt
     1381 ggtattttgc tatttggact gaaatattat actgaaggaa gttatggaga aatccctggt
     1441 atacttgatc agctaaatcc attagttgaa aaaggagagg tctatgtaaa gacacaaaaa
     1501 ccagaggaag taaatgaata tggtacagca aggtatgtgc agaaagctgc tgatgccaat
     1561 gggaaagaac gaacggttga atttaatggt ctttctgtgt taaaagaaaa tcattatttg
     1621 aaaattagca ataaaggagc acacatagaa acgtatgagg aagttcctag agaaaaagtt
     1681 ccggagaaag cattagcaat tattgggtaa atcagttttt gtattaagaa tacgcccgat
     1741 tttattattc ataatttaat tatgtaaact aaatacaagt agtaaaaata atacaataat
     1801 ccccctgcaa aaagattgtt cttttacagg gggttcattt tttcagcaag cacatcaaat
     1861 tgggatggca gggttcgaac ctacaattgc ttgattcaga gtcaagtgcc ttaccagttt
     1921 ggctacatcc catttattca acaaaataat catagcactt tcctaaaaaa acgcaagtta
     1981 tttttcttga tcggatagtt gttgctttac tcgtaaaagg cggcgtacta gtttcttttc
     2041 taatccagca ggaagggcaa tcagttctgc tactgacaag acggtgtcca ataactgata
     2101 ttctttgaca ggttcattca taatatgata gatgtttgct aaacgttcaa tatattcaaa
     2161 gcgagtatac tctgttcgag caattagaaa tttcaataaa tcggtggctt tctcgtactc
     2221 ttgatctgcg taataaacaa gagattgacg atacaatttg atttccagtt cattgagggt
     2281 agcaggtctg cgacgctcgt tccactcatt aatagagact ttaccttgaa catttccctt
     2341 gcagtcacgg acaaaaataa tcggaatttc atttggatcc atttttttat gtgtgaattt
     2401 ttccattatc tacgctccct tcaaaggttt ggtatcttga gtataaggga tttttgtttg
     2461 aattactagc ttttctccaa agttagcatc tgtccaccca ttgcagtgaa aatcgccgaa
     2521 aaatcgttga tcggtactgc tcgatttttt acaccatagg gatcgttgac ataaatattt
     2581 tctttgtcat atccagtgac tacacaagca tgaacaagag gagtgactct tacttctcca
     2641 ttatttgtgg tccaagtcat aaaatcagtt tcatctggga cttcaaaatt gatagtcaca
     2701 gaagtccaga caggatttcc tttagatagt gcttttttga tatcatcgag ttgagcatcg
     2761 gtttttggaa aagtgtgata atccgtttgg acgatttgct gagcaaggga ggcaactggg
     2821 ggaacataga cgaccatagc ccagtctccg ccactgatat caccaacaaa tccttcatta
     2881 ggatcgccat gagtcccatc tgcattgaaa acagggacgt aatccaattg ctcagctaac
     2941 tggtttttag ttgtttcata accataaaaa gataaaagca tagaaagcga agtgacttca
     3001 cagccatttt ctaaagaagg agaatcaaac tggctctcta atggaacatc tagtaaaact
     3061 tctgctttct cttttgtctt tttcgttttt tgtgttgaac ttttttgtgc agtgttcatt
     3121 gtatgttctg gagaatggat atgtaggtaa taacttgtac cgcttaataa aagagcagct
     3181 gtgcccccta gaaacaatag ttttttgaac gtttttttct tcatttgaaa tctccctttt
     3241 cagctgttta tctttcctat tataaatgta tttttattgg aaatggaagt attatatttt
     3301 aatatgtagg aaataattag tttttaagga atatgtttta attttttgaa aaaaattctt
     3361 ctaaatacaa cagctgtttt aattgtgaaa acgaaaacag tacgctataa tgcctgtaag
     3421 gttgattttt aactgtaaat aaaaatcaat gagttaaatc tccaaagaaa tagcagtggt
     3481 aatgtggaga aaagttccag cagtttagga ggattttgat gacggaagaa atgattaatt
     3541 taggagaaca atatgcatgc aaaccaattg gttttacaaa aacggtcatt ggtgaagttg
     3601 tatcaaaaat gacaaactgc gccgtggtca aagttgccca atgtgctgca gaggaccaag
     3661 aattattaga tgaaaaagca agcatggttg ttgcaaaata tgatacattt gaataaagaa
     3721 aacaattgga cgtgcggcgt gctgcaagtc caatttcttt gagaaggtaa atcagtattc
     3781 ttttcctatt ttctggagaa gaaaaagcga ggcgagttgt catggtaaca ttctttacgg
     3841 ttctattgtt tttattcgtt gttgtgatgt ttttatttct ttggctggtt cgaaaagaaa
     3901 ttatttatcg gactgtgcga aatcgatggg tctacttggt gatacccttc ttagtgtcac
     3961 tagtcatttg gtatacactc atcagtcatc ctacagcaga tgaattggca aaagggatct
     4021 tgtcagcaat gatttttatt agttttttgt tggacagtag aggaatcaca gaagaaggac
     4081 ttgttttgaa tagctttgac aaaaaaggtg ttcctttttc tgagatcaat aaaattgtat
     4141 tatatcagcc aaaaggttct aaaatcgtaa aaatgaattt tttccgaaac ggatggcgag
     4201 gaccgatgct gaaattttct gcatcattag aagaattagt tccatttttg tcccaacgct
     4261 taaacgatga ggcagagatt gatattatga ttgatccaga ataaaaacag aggttgcgac
     4321 aagatgtttg tcacaacctc tgttttttat ttcaaataaa attatccaaa tacgcggaag
     4381 cgatcggcat cgttcatgta ttctttttcc tcagccggtg tttctggtga accgaatact
     4441 aattgcgaac gtagtttcca gtttgaaggg atattccatt cttttgcaac tgcttcgtca
     4501 ataactgggt tgtaatgttg caagtttgcg cccaatcctt tatcaactaa tgctacccaa
     4561 gtattagctg ttgcgattcc gtttgattgt tctgaccaat ctgggaagtt gtctgcatat
     4621 aattcaaatt gttcttgtag accttttacc acgtcagtat ctttgaagaa taatactgta
     4681 ccataaccat ttttgaaacc agctaatttg ttttgtgtat ttgggaaagc ttctgctgga
     4741 gtcaatgggc gaagtgcttc ttcagtgatt tcccaaagtt tttcatgtgc atctccgaaa
     4801 aggataactg cgcgtgttga ttgagcattg aaagcagttg ggctttcttt aattgcttct
     4861 ttgatcaaag ttgttaattc ttcattagaa agtgaaacat tacgtcctag atgatagatt
     4921 gaacggcggt ttttcaaagt gtcagtaaaa gttgtcataa taaaatttct cctttgattt
     4981 gcgttttgca tttttttaat tgattacgaa atgaagtata gcaccttaca aaaagtaagt
     5041 aaacagatat gctcacaaaa aataagcgaa gttcaaaaaa caattttaat aagaaaaatc
     5101 gttatgtaag gattttctta gttatcgctt tcattgttgt gttttaggaa tttttgc
//
LOCUS       DASJST010000165         5105 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJST010000165 DASJST010000000
VERSION     DASJST010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.167"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..608
                     /locus_tag="U4B05_002589"
     CDS             <1..608
                     /locus_tag="U4B05_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051679.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="U4B05_002590"
     CDS             628..1656
                     /locus_tag="U4B05_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2051680.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="U4B05_002591"
     CDS             1653..2411
                     /locus_tag="U4B05_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2051681.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="U4B05_002592"
     CDS             complement(2497..3237)
                     /locus_tag="U4B05_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2051682.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="U4B05_002593"
     CDS             3298..4551
                     /locus_tag="U4B05_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2051683.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="U4B05_002594"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="U4B05_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
BASE COUNT         1737 a          894 c          986 g         1488 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttct
//
LOCUS       DASJST010000166         4937 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJST010000166 DASJST010000000
VERSION     DASJST010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.168"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(163..1773)
                     /locus_tag="U4B05_002595"
     CDS             complement(163..1773)
                     /locus_tag="U4B05_002595"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN2051684.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2966..4306)
                     /gene="glnA"
                     /locus_tag="U4B05_002596"
     CDS             complement(2966..4306)
                     /gene="glnA"
                     /locus_tag="U4B05_002596"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN2051685.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4334..4726)
                     /locus_tag="U4B05_002597"
     CDS             complement(4334..4726)
                     /locus_tag="U4B05_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2051686.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1461 a         1059 c          803 g         1614 t
ORIGIN      
        1 tttgcaaaaa ataaaatatc caatagcgct ttaatcgaaa aaagtctttt gctttctatc
       61 ttttttccta caaataataa caaatcagaa aaagagaaaa gtgacaagaa aattgcttat
      121 ccccttctct tgctactttt ctctttctat caagttcaaa aattattttg tttcgtgatc
      181 tgctaatgat gctccgacaa atgctttgat taatttttgc gggcgatttg gacgagaaat
      241 caattctggg tggaattggc atcccacaaa gaattttttc tctggcagct ctacgatttc
      301 tactaaacgg ttatctggtg agacacctga aaagactagc ccttgttctt caaacaattg
      361 acgatatttg ttattgaact catagcggtg acggtgacgt tcttgcacaa catcggctcc
      421 atcatatgct tcagccgtct tcgttccttt tttgatttta catgggtata atccaaggcg
      481 taaagttcca cccaaattct cgatgttttc ctgatcagcc attaaatcaa taatattgtt
      541 tgctgtatct ggattcgttt cagctgatga cgcatcttct aatccaacga cgttacgtgc
      601 aaattcgaca caagccatct gcataccaag acaaatacct aaaaacggaa cgtcattttc
      661 gcgagcataa cgaatcgctt cgattttccc ttctactccg cgatcaccaa atccaccagg
      721 aaccaagatt ccatctgcgt ctttcaaacg ttcttctaca ttttctctcg ttaattcttg
      781 tgctttgatc caatcgatct cgatgtctga attataggcg aaaccagaat gtttcaatgc
      841 ttcaactact gagatatatg catctggtaa ctcgacatat ttaccgacaa gcgcaatccg
      901 tacttttttc ttcaagttca agacacgttc ttctaattct ttccattctg tcatgtctgc
      961 ttctggtgta tctagtttta aatggtcgca aacaatttga tccattcctt gtgcttgtaa
     1021 aactaatggg atggagtaaa gtgtatctac atctggcgac tcgataactg cttctggttc
     1081 gacatcacaa aattgtgcca atttgttttt cacgctttga gaaacaggtt gctctgtacg
     1141 taccaccaaa atattcggtt ggatacctaa accacgtagc tcttttacac tgtgctgcgt
     1201 tggtttagtt ttcatttctc cagcagcttt caagtaagga atcaatgttg tatggatata
     1261 cataacatta tctgtaccga catctgcttt catttgacga agtgcttcaa ggaatggcaa
     1321 agattctata tcccctactg ttccccctac ttctgtaatg atcacatctg aatcagtcat
     1381 tttagctgct cgcatgattt tttctttgat ctcatttgta atatgaggaa taacttgaac
     1441 agttgctccc aagtattcgc ccttacgttc tttacgcaaa acttcagaat agatttttcc
     1501 tgtcgtcacg ttggagtatt tgttcaaatt gatgtcaata aaacgttcgt agtggcccag
     1561 atccaaatct gtttcagcac catcatctgt tacgaatact tctccgtgct gatatgggct
     1621 catcgtcccc ggatccacgt tgatgtatgg atcaaatttt tggatagtta cttttaagcc
     1681 gcggtttttc aataagcgtc ctaaagaagc tgcaacaatc ccttttccga ttgatgaaac
     1741 gacaccgccg gtcacaaaaa tatattttgt cataagttca aaaactcctt tttaatttat
     1801 gtgcaggaaa agtctcagac aataaacaaa gctccctatt cttcaataag aatagggagc
     1861 cgaaatttct gtaaacttct gaccctctaa aagggtgccc aaggtgtata atacaagctg
     1921 actaagggaa agtcaagtac tcaaatgaat actatccgct tacaattttt tctttttcga
     1981 tatcctttga gaaattattt cttaagtcct ttatgagtgc aaaaaaaatt taacccacat
     2041 aataagcttg atataaacat agctaacgtt aaacctaagc aaatcagaaa atcgttactg
     2101 aaaaatttta agtgaaataa tagataaaaa aataaaacaa acattatgat agataatatc
     2161 ccacctaaca aagaactact ttttcctact tgcagcaaca gtttatatag cttatcgtag
     2221 aaacaatctt tttctttcac ccttatttac ctcctttacg taaaaaacgt agtccacagt
     2281 tctcggattt ttggcatatc ttttctatta aataatatca aaaacaaaac aattccacta
     2341 attttttcaa agatgacctt tttttcaaca aaagtgcctt gtagggattc attgaaggta
     2401 acgaatcaca taagtaaaaa ataaggagat gcgacaaaag tcgtgcctcc ttatttctaa
     2461 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat caaacatgag
     2521 aagctgtttt tgaaattatt tcttgtgatt cggagttaaa tggtaggctc acaacctctg
     2581 gataaacggt attcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     2641 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacgtctttt tcaaaacctc
     2701 ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gttcagccaa ttcaaaaaca
     2761 agaaaagctg tttttgcttg gctattctta ttacttgttg cagggcagct ttttcacaac
     2821 ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
     2881 tatgaggaac tatttttccg ttagtttctc taatactcag gtcaaaacgc tgatttcaca
     2941 gcctctaatt cacggtattc ctgttttaat aaagttctag atattgttct ctttcccact
     3001 cggaaacagt ttgtctgaat gccgcccatt cgagacgttt tgcttcaaca aaatttgaga
     3061 agatatgttg tcctaatgcg tcaatcatta cattatcctt acgcaattct ttgatagcat
     3121 tatggatagt tgaaggtaag tcctggatat gtgcagcttc tctttcttcc tcatccatta
     3181 cataaatgtt acggtcaaca gctgctggcg gtgtgatctg attacggata ccgtctagtc
     3241 ctgcttgcaa caatactgcc attgccaagt atgggtttgc agatggatca actgaccgca
     3301 attccaaacg tgtggataaa ccgcgagatt ctggtacacg tactaatggc gaacgattac
     3361 ggccactcca tgcgacataa acaggcgctt cataacctgg aacgagacgt ttataagaat
     3421 tgactgttgg attgcaaacg gctgtgtagg cacgagcatg tttcagcaaa ccacctaaga
     3481 aatgataagc tgtttcactt agttccaatt caccgtcttt atcatagaaa acatttcctt
     3541 catcattgaa caaagacata ttgcaatgca taccagaacc attgatccca aataacggtt
     3601 tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat cgttttgaca acgagcttga
     3661 aggtttggat attatcacaa gcatctacca catctgcata tttaaagtca atttcatgct
     3721 gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa gcctagactt tctaattcca
     3781 acacgatgtc acggcggcaa ttttcaccta aatctgttgg tgcaaaatca aaataaccac
     3841 ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc taatttaaat aagaagaatt
     3901 ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc gcgcatatct ttcaatgctc
     3961 gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc atccggatta tagatatcac
     4021 aaattaaacg agcaactttt ccatgagcac tttcccacgg gaagatcatc caagtggaga
     4081 gatctggata caaatacata tcgctttctt caattcgaac aaatccttca atcgaagaac
     4141 cgtcaaacat cattttattt tctaatactt tgtcgatctg gctcactgga acttctacat
     4201 ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa aaatcgtacg ttttcttcct
     4261 caatgattcg tttgatttct tcacctgtta tctgtttctt taccatggta tccctcttcc
     4321 ttttttaatt tattcagtca aagaattgtc tttgcaatcc ttgcgactga taatgacctg
     4381 ttttgcttaa cccaccttgc gccaaaatct catcacgaaa aatttgtcga acgtcatgat
     4441 ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc ctgtgccatt tcgtatacac
     4501 gtttgatccc agccatattc aatccctctg aaagatagtc tttaatctca agcaacatat
     4561 caatatcatt cagcgaatac atacgtcggt ttccttcgct tcgttccgga tgaactaaat
     4621 cttgttcttc ataatagcga atttgccgag cagacaaatc agtcagcttc ataactgtac
     4681 caattggaaa tacggacata gatcggcgca attccttttc tcccatcaga cgtccccttt
     4741 cgttttataa gttggctaaa gtgtaccaat taaaaaaaat attgtcaact gcatatgtta
     4801 ggttttctaa catgagatct ctatttttta taaaataaca aaaaaataag aacacaaaaa
     4861 aggtatctag ttgattttcg taccagatac cttttatttt ttattatact gtactatcct
     4921 ttattagtgt ttttatt
//
LOCUS       DASJST010000167         4883 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJST010000167 DASJST010000000
VERSION     DASJST010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.169"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            509..2353
                     /locus_tag="U4B05_002598"
     CDS             509..2353
                     /locus_tag="U4B05_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2051687.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2409..3875
                     /locus_tag="U4B05_002599"
     CDS             2409..3875
                     /locus_tag="U4B05_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2051688.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3912..4754
                     /locus_tag="U4B05_002600"
     CDS             3912..4754
                     /locus_tag="U4B05_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2051689.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1605 a          785 c         1028 g         1465 t
ORIGIN      
        1 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
       61 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
      121 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
      181 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
      241 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
      301 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
      361 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
      421 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
      481 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
      541 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
      601 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
      661 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
      721 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
      781 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
      841 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
      901 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
      961 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     1021 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     1081 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     1141 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     1201 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     1261 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     1321 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     1381 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     1441 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     1501 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     1561 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     1621 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     1681 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     1741 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     1801 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     1861 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     1921 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     1981 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     2041 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     2101 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     2161 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     2221 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     2281 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     2341 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     2401 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     2461 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     2521 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     2581 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     2641 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     2701 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     2761 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     2821 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     2881 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     2941 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     3001 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     3061 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     3121 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     3181 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     3241 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     3301 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     3361 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     3421 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     3481 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     3541 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     3601 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     3661 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     3721 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     3781 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     3841 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     3901 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     3961 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     4021 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     4081 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     4141 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     4201 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     4261 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     4321 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     4381 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     4441 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     4501 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     4561 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     4621 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     4681 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     4741 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     4801 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     4861 ttaacgtaag ataataaaaa atc
//
LOCUS       DASJST010000168         4866 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJST010000168 DASJST010000000
VERSION     DASJST010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.170"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            298..1221
                     /gene="truB"
                     /locus_tag="U4B05_002601"
     CDS             298..1221
                     /gene="truB"
                     /locus_tag="U4B05_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN2051690.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1225..2166
                     /gene="ribF"
                     /locus_tag="U4B05_002602"
     CDS             1225..2166
                     /gene="ribF"
                     /locus_tag="U4B05_002602"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN2051691.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2518..3303
                     /locus_tag="U4B05_002603"
     CDS             2518..3303
                     /locus_tag="U4B05_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2051692.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3316..4167
                     /locus_tag="U4B05_002604"
     CDS             3316..4167
                     /locus_tag="U4B05_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN2051693.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4291..4743)
                     /locus_tag="U4B05_002605"
     CDS             complement(4291..4743)
                     /locus_tag="U4B05_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051694.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         1615 a          797 c         1048 g         1406 t
ORIGIN      
        1 aacggaaaaa agactggaag aatggccagt cttttttttc gtttttggga agtgaaaacc
       61 aaactattta cctgtaagcc acccataaat ccaatcaaat ggtgcaaaga aaaaatcttc
      121 cattctattc cctcctttaa aaaagcataa catgatttct atttacgtga gcatgaattt
      181 tgttacgttt ttattgccga aaaataaaaa ccgttcatta tgatgtcaaa ttcgtcagta
      241 attgatgcaa tgtgttataa taattcagtt aaatcaaaaa tgaaatggag aaaagacatg
      301 gacgggttat tacctttatg gaaagaacgc ggaatgacaa gtcatgattg cgtattcaaa
      361 ttaagaaaaa tcttacatac gaaaaaaatc ggacatgggg gtacgcttga cccagacgta
      421 gacggtgtgt tgcccatttg tattggaaaa gccacaaaag ttatcgaata tttgacagat
      481 tctgggaaaa tctatgtagg cgagataact ctaggtttca gtacaacgac ggaagatcga
      541 tccggagaac gggtagaaac aaaaccagta gatatgtctt tatctgaaaa aaaaatagat
      601 gaagtgatgg cttcatttat tggtgagatc acccagatac caccaatgta ctcagcggta
      661 aaagtcaacg gtaagcggtt atatgaatat gcccgcaata atgaaacagt ggagcgtcct
      721 gttagaaagg ctcagattta ccgttttatc cggacaagtg aattgacaag agacgaagtg
      781 acaggaacca tttcttggcg cttcgaagta gaatgcggga aaggaaccta tgtccgcaca
      841 ttagctgtag atacaggcaa gaaacttggt taccctgcgc acatgtctga tctgacccga
      901 acagcaagtg ccggtatgtc ccagaaggat gctctgacat tagaagaagt agctcaagct
      961 gtgaaaaatg ggacaatcga tgattgtttg ctgccaatag aaaaaggagt ggaacggttc
     1021 aaacgagtag atattactga tgaagtatgg cagaaagtaa aaaacggtat gagacttgat
     1081 tatactgtat ttgcgctccc agaaatgccg tcagaagaaa ttgctttgtt ttataaagga
     1141 aaagttgtca gtatttatca gccaaacccg aaagaaaaaa acaaattaaa acctagtaaa
     1201 gtattgagaa atgaggaata acgaatgaat atcatcaaga tcagacatcc ttaccaatca
     1261 gaacaaattc cagaagacga tgtcgtttta gtgctgggat ttttcgacgg cgtgcatatc
     1321 ggacaccaga aagtcataaa gacaggtatg gaaattgcac aaaaagaagg attgaaatta
     1381 gcattgatga ctttcaatca gcatccatct attgtcttta aaaagatcga tcctagcagc
     1441 gtgaaatatt tgactagcct taagcaaaaa gaagaaaaaa tggccgcttt gggtatagat
     1501 tatttatatg aagttgattt tacttcttct tttgctcatt tagcaccgca ggaatttgtt
     1561 gatcaataca tcgtagggtt acatgcaaaa tatgctgttt ccggttttga ttatacgtat
     1621 ggaccaaaag acattgcaga tgtcgctcat tttcctgaat atgccaaagg aagattcaaa
     1681 atcgtcacag tttctaaaga agaagaacaa ggtgaaaaaa tcagctctac ccgtattcgt
     1741 ggattactag acagcggaca agtaaaagaa gcagcaaaac tattaggttg cacatatgag
     1801 gtagaagggg tagttgtcca tggcgatgca cgaggcagac tactaggatt ccctacagca
     1861 aatgtcaaag taaagagcac ggttcatttg cctaaagaag gtgtttatgt gtcggaaata
     1921 aaaattgggg aaacatggta tccagccatg ggttcaattg gacataatga tacattcggt
     1981 gaaggacggg agctgactgt agagttgtat attttggact ttaatcaaga tatctatggt
     2041 gaacatgtcg cggtacgctg gaatcaattt ttaagagatc aagtgaaatt ttctagtgta
     2101 gaggaattga ttgaacaact caaggaggat gaacaagcaa cagctgaata tttttctcag
     2161 caataaaaca ttatgcataa aatctcttgt ttatgtataa ctagcaatta caataaaatg
     2221 ggtgataaat aagcccagga aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc
     2281 aaataaatgg tttttcagaa gtagcttctt cggaaataag ccgaaatttc ttgaagctga
     2341 ccacttctgt cacaacctct ttctttatag aaataaaagg acactagcaa ttcatgaaaa
     2401 cgattgctag agcgtatcat ttgctttaga ctatatatct tggtgtacac tcactttaag
     2461 gcggaaataa aagttatccg taataacagg tttaggtatg aaaaaggagt gggtactatg
     2521 tacaaggaat tagaaaacaa agtcgcagta gtaactggtg gctcaaaggg gattggaaca
     2581 gcgattgctc aacgattcgg gaaagaaaaa atgaatgtag ttgtcaacta taactcggat
     2641 aaagaaggag cagaaaaagc agtcaaagca atcgaagagg caggcggaaa agcagtctct
     2701 gtacaagcag atgtcagttc tgaagaagga atacagcgtt tacttgatac ggctttagaa
     2761 aattttggga cactggacgt ttgggttaat aatgctggga tggaaaatca gcatccaacc
     2821 catgaactta gccttgaaga ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt
     2881 atcggaacga aagcggcttt aaattacttt acagagaata ataaaaaagg aaatatcatc
     2941 aatatgtctt ctgttcatga acagatccca tggccaacgt ttgctcatta cgctgcttca
     3001 aaaggcgggg taaaattatt taccgaaacg gtagcaatgg agtatgcacc gaagggaatc
     3061 agaatcaata gtataggccc tggtgctatc cgtacaccta ttaatgcggc aaaatttgat
     3121 gatcctgaac aaaaagaact cttggaaagt atgatcccac taggacgcgt aggtgaacca
     3181 aaagaaatag cggcgacagc tgcatggttg gcttcagacg aatccagtta tgttacaggt
     3241 attacactat ttgcagatgg tgggatgact ttatatcctt ctttccaagg tggaaaagga
     3301 taagggtgaa taaaaatggg tttattgatt gcattgatcc cggcagttgg ctgggggatc
     3361 cagccactga ttttgaaaaa gattggtggc agaccaacaa atgagatatt aggaactggt
     3421 attggagcgg tgcttgtcgg cttactcgtt caattattcg cttctccagc tggtatttca
     3481 gtgactacat ttttgattag tttactttct ggtgcatttt gggttattgg acaaacagga
     3541 cagtatacat ctttcaactt gattggtgtt tctaaaacga tgccaatttc gactgcatta
     3601 cagttagtcg gaacctcgtt gattggtgtc ttcgcttttg gagaatggtc tggaagtatg
     3661 gggaaaatca ttggagcagc agcaatcatt cttcttgtat ttggttctgc tttaacggca
     3721 atcagtgatg atcctggaaa aaaaggcggt atcacaaaag gaattagtct attagcatca
     3781 acaagtattg gttattgggt atacagtgca ttgcctaaac tagttgatgc aagcggactt
     3841 tccattttct ttccgcaaat gatcggtgtc tttttagggg cagtgattta tgtgattttc
     3901 cgtaaaccaa ccgcattttc tgaagacaag agttggaaag caacgatcgt tggagccatc
     3961 tttagtgtgt cagcattggc ttatattttt tcagcaaatg aaaatggggt tgctactgca
     4021 tatatcatta cacagctaaa cgtagttatc tctacattag gcggtatgta catcctgcat
     4081 gaaaataaga caccaaaaga aaaaaaatta acgattatcg gattattgct gatcgtagta
     4141 ggaagcatgg tcacggtctt tatttgagga taaagatggt aaaatttcat ttattaaaaa
     4201 aatatttgtt tgaaccaaac agtaagtact tataaaaaaa tagcgctaga atttccaaca
     4261 tctctggaat ttctagcgct ttttaattgt ttaagataca agggaaatat ctgtctggat
     4321 caataatttt tcattgatcc attgactaaa gtttacgaca taattgaagt taccaccgat
     4381 tagtatctcc gcatgattgt ctttataaag atcgtattgt aaaagacggt cttttttatt
     4441 tgcgtaaaca tcgttatggt actggactaa ttgttctcgg aattgattga ttgctgtaaa
     4501 atcacgatat tgaaggttga aacgcatcgt gattggtgca tttagcatga atcgctgcat
     4561 ttcttttgct gcttcctctg cttgatctcc gtataggaac tcaaaaagat tacgttcaga
     4621 aaatttccat tgcccgccaa cctttgttcc ttttagtttt ccttcatgaa gataatttct
     4681 gatagaacgt gtagtaacac ctaattgttc ggctaattca tttactgagt aaaaatcttt
     4741 caatgttcac cctcctatgt gaatggttct actgacttag ttgatatttg cttaatcacg
     4801 aaatgtagaa agaacgattt taattaaggt cattgtaaaa atcaagtcag aaaattctaa
     4861 ctttta
//
LOCUS       DASJST010000169         4851 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJST010000169 DASJST010000000
VERSION     DASJST010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.171"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            184..639
                     /gene="tnpA"
                     /locus_tag="U4B05_002606"
     CDS             184..639
                     /gene="tnpA"
                     /locus_tag="U4B05_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN2051695.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            904..1002
                     /locus_tag="U4B05_002607"
                     /pseudo
     CDS             904..1002
                     /locus_tag="U4B05_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1685..2503)
                     /locus_tag="U4B05_002608"
     CDS             complement(1685..2503)
                     /locus_tag="U4B05_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2051696.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            2664..3353
                     /locus_tag="U4B05_002609"
     CDS             2664..3353
                     /locus_tag="U4B05_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2051697.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            3367..>4851
                     /locus_tag="U4B05_002610"
     CDS             3367..>4851
                     /locus_tag="U4B05_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2051698.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQI"
BASE COUNT         1631 a          728 c          885 g         1607 t
ORIGIN      
        1 aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt tgtgcgtaaa
       61 gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt tagcgataaa
      121 aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga aaggagtttt
      181 ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa gtatcatatt
      241 atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa agatcttgga
      301 aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc acatgcaatg
      361 ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc cagttttatg
      421 gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa tctcaaatat
      481 aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt cggattgaat
      541 caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat tcgatatatt
      601 ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag tgagtgaaga
      661 tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt cgaccccttc
      721 tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata gacttgattt
      781 taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa tttacggcta
      841 ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat tagaaaatgc
      901 tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt ggaagtattt
      961 ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag agtatattta
     1021 cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat aaataaaatc
     1081 tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt ttaacaagtg
     1141 atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg aagtattttg
     1201 caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa caagatgatt
     1261 gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa acattcgctt
     1321 agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt cctgctcatc
     1381 gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga gaggatgatc
     1441 cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt tctttcaagt
     1501 tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg taggaactac
     1561 agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat caagtatgct
     1621 ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc taagtggggg
     1681 tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt aattcaactg
     1741 aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata taaagttgtg
     1801 atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt tgtaaaacta
     1861 agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata atttttgcgc
     1921 catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca gataaagaaa
     1981 aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga aaatgagggt
     2041 ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa tcaagactag
     2101 tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg atcattttaa
     2161 tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga gcttctgtta
     2221 aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt ttttgactaa
     2281 agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca attttttgac
     2341 aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca ctcatcgtac
     2401 tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt tcagacttcg
     2461 ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact cctctacaat
     2521 ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata ctccattttt
     2581 atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc caaaaataat
     2641 aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt atttcaaaag
     2701 gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag gaagaaagtt
     2761 taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata cgtgcaaatt
     2821 ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt ggaattatta
     2881 aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa gtagatgaat
     2941 tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat cgacatgatg
     3001 gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat cagttattta
     3061 tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga atagattttg
     3121 taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg ccagatatag
     3181 atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga aggattcata
     3241 ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt gtgggtggtg
     3301 ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt tagggggaga
     3361 aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc aattgctata
     3421 ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga gagtgctata
     3481 aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat tatgagttat
     3541 gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat tgctgtagga
     3601 cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta tctagtatat
     3661 actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac actagataca
     3721 ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa tcgttataga
     3781 aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat tccgattgtt
     3841 tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc accaattcaa
     3901 aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg aactttttta
     3961 tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat ttaccctttg
     4021 ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat tgctggagcc
     4081 caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt tacttatggt
     4141 actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc agcatgttat
     4201 gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct ttattttagc
     4261 ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat gtttttattt
     4321 gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt ttattttgct
     4381 gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga ctttttatta
     4441 ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc ttttggtatt
     4501 gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt taaggtagct
     4561 attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa caaaaataca
     4621 tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga aaatattgag
     4681 tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga gattattcaa
     4741 aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg tggcatccaa
     4801 gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat a
//
LOCUS       DASJST010000170         4823 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJST010000170 DASJST010000000
VERSION     DASJST010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.172"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(285..2951)
                     /locus_tag="U4B05_002611"
     CDS             complement(285..2951)
                     /locus_tag="U4B05_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2051699.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3292..4743
                     /gene="cls"
                     /locus_tag="U4B05_002612"
     CDS             3292..4743
                     /gene="cls"
                     /locus_tag="U4B05_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2051700.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1417 a         1027 c          869 g         1510 t
ORIGIN      
        1 ttgatgaata ataaaatgat tggctttccg tctatcataa ataaaaaaag ggactgtgac
       61 caaagtcgtg tcaaagtccc tctttccgaa taaacagtgt ccaatcagat gaccctcagt
      121 attagctcaa aatgctgatt ttacaacttc ttccttaaac ggtgttctcc tgccaactct
      181 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg tttgttgaaa ttgcaccttg
      241 tgactcagaa ttaaacggca ggctcacaac ctcgttgatt cgttttattt atttccttgt
      301 actcgtacaa tgatcaattt agtgatttcc atcaaaacaa ttgtcacaag tgcgattccc
      361 atacatgtaa agacttctgt caatccaaat gctgatggga tagagaagac ttctttcagt
      421 cctgggatca ttgttaagca gtaaagcgcg ctacatacaa ctacagctag taagacactt
      481 ctgttagaga agaaccctgc ttggatagct gtttgtgtgt ttgaacgtgc tgggaatgtt
      541 tgcaatgtcc gacaccaaat caatgttgaa aatgacatgg ctacaccgaa ttcaggtgaa
      601 tgctgcatgc caagccattg agcaaagata acagcaatcc caatcagcac accacgatac
      661 gttacagaaa tcaatgtttt gtccgtaaag attcctgaat ttggatctct tggcgggcgg
      721 ttcatcgtag caggttcagc tttttctgta cctaatgcaa ttgctggcag tgaatcattg
      781 actaaattga tgaacaacag ttgtaatgct gtaaatggat tggaccagtc agcaactaag
      841 gcaaagatga tcgcaatgat cgcacctaaa tttcctgcaa atagataaga aatagctttc
      901 ttgatattat caaacacatt acgtcctact tctactgcgc tgacaataga agcaaagtta
      961 tcatccgtca ataccatcgc tgctgcatct tttgccacat ctgtacccgt tcccatagca
     1021 attccgatgt ctgcttgttt caatgctggt gcgtcattta caccatcacc tgtcatagct
     1081 gagatatgac ctttattttg ccaagcacga acgatacgaa ttttattctc aggtgaaaca
     1141 cgtgcataaa cagtgacttt ttctaaaact tcgtttagtt gatcttctgt caattcatct
     1201 agttccgtcc cggtcaaagc caggtttcct tcttcaaaaa tcccgatttg ttttgcgata
     1261 gcaacagctg ttgttttgtg gtcaccagta atcatgattg ttttgattcc tgcacttctt
     1321 gcttctgtta ccgcctgagt cacttcagca cgtggaggat cgatcatagc aagcaaacca
     1381 acaaggatca aatcgtcttc atcttcaaat gataaatcaa aattatcaag tggacggtaa
     1441 gcaaaagcta gtacacgtaa tgcgcgtgtt gaaaaagctt cattttgctc ttggaatttt
     1501 ttcatcaatt catccgtcat cggaacgact tcaccattta gcaatacttt tttactgcgt
     1561 tggaagacga tatctggtcc gcctttaaca aacaagtaat ttttgttatc aattgtgtgt
     1621 ccagtagaca ttaattttcg atctgagtca aaaggaagtt ctgcttgtct tgggtagttg
     1681 tttcggattt cctgataagg ctggtttaat ttttcactaa agtcgataaa cgctacttcc
     1741 gtagggtcgc ccaatttagt accgtcttca ctgatcgatg catcattcgc taaaacactg
     1801 atttcgatca agcgacgttc gtcagctgtc cattttgaag ggtcatttgt gaaatcccca
     1861 gtatcgccat tacctaaaaa gtagtcaaca actgtcattt tgttttgtgt caatgtacca
     1921 gtcttatctg tacagatgat acttgtcgaa ccaagtgttt caactgctgg aagcttccga
     1981 ataatggcat gacgttttgc cattttattc gttcccaaag ataacacgat tgttacgata
     2041 gactgtaatg cttctggaat agcagctacg gctacggcta cggcaaacat cactgcgctg
     2101 acgatatctg cagtcatatc tcctgtacct tctccaagat aaatacgaaa cagttggata
     2161 cctaaaatca ataaagacaa tgccaaaata gccaaactta gttttttact gaatttatcc
     2221 agacctcgtt gtaacggagt gacttgctca gttgttgatt ccaaaaggcc agcaacttgt
     2281 ccgatctccg tattattccc agttgaagtc actaagaatt taccgcgtcc gtaagttacg
     2341 atcgttccac tgaataccat atttgtacga tctccgattg gtaccgtact tgtgatatcc
     2401 gttacttctt tttctgcaga agtcgactct cctgtcagca taccttcatc tacttttaat
     2461 gatcccgcat ctagtaaacg tccatcagca ggtacataat cgcctgcgtc taacaacacg
     2521 atatctccta ccacaagttc gtttgccgga atcgtttttt ccttaccatc acgaatcact
     2581 cgagcatcgg gcgctgaaag gtttttcaac gcttctaaag aaccttctgc tttttttgtt
     2641 tggacaacac tgattataga attcaacatc aatactgcaa aaataactaa gaattcgatg
     2701 tagtcaccca tgaccatctg aatcagtgcg acaatcagca atacgataac cattgcatct
     2761 ttgaaagttt ctaagaacaa tttccaagtt ggcttcttcg gcttttcttt tagctggttc
     2821 aagccatctc tttctaattt ttccttcgct tgcgtggtgc tcaatccatc ggttgagctc
     2881 tgcgtttgct gcatcaacga gtcgatcgtt tctttataga ccgcttgttg tgcttgttcc
     2941 ttgttttcca aatttcatcc cccatttttt gatcttggcc ctagtcctgt ctgaaaaaaa
     3001 agagacctaa gacaaatacg catttgtctt aggtctcacc atttcataca ataccagaaa
     3061 accatttggt taacttactg ttgatattat cgcaacaaac gttgccggtt actcccctaa
     3121 ggagatattc aactgtacta atatcctacc ttattaacct acaaaaagca actactttag
     3181 cccgatttac ctacattttc atgaaaggag tgaaaagtat attcaccttg gtaggcttct
     3241 ttcttctatc atgctatact aatgttattg tatgtaagga ggaacatttt tgtggtatct
     3301 agtattataa ccgcccttta tctattaaat gcacttattg ctttggtggc tattttgatc
     3361 aaaccccgag atgtagcagc catttgggca tggctcttag tattgtttgc ccttcctggg
     3421 gtgggctttg tattatattt atttttcgga cgtggattaa cggataagaa aaaattctat
     3481 ctccgacaaa gtgacttgaa agaattagaa aactttcagt cttttagagg agataccatt
     3541 gaacattacg atcctgacat gggcgataaa gacaaacagc aatttgttga cttcttctct
     3601 tcattaaatc gtatgccgct gacaagaatg aattctgtca ctcttctcac agacggacaa
     3661 gagaaattgg attcactgct tcaagatcta aaaaaagcca aacattcgat ccatatcgaa
     3721 tattacgcat ttgtgacaga taatatcggc cagcaagtct tacatgtttt agaagaaaaa
     3781 gccgcagaag gcgtggaagt tcgaatatta tatgatgcat ttggctctca tggcacaaaa
     3841 gcaaaagatt tcaatcgtct aatcaaaaat ggtggacatg tccatacatt tgttacctca
     3901 caaagggcat tacttcgttt ccgattgaat taccatgatc accgaaaaat cgttgtgatc
     3961 gatggaaaga ttagttatac cggtggtttc aatattgcca atcaatatgt aaatacaaca
     4021 aaaaaattcg gctattggcg cgatacgcat atacggattt tcggtgccgc ttcttctttg
     4081 ctccagcttc gcttcttaac agactggaac gtctcggtac ctgaagaaaa aaaggtcggc
     4141 tatcatttga attatttctt taaaaaagca gatcgagatg aatctaagct tgctgataca
     4201 tccatccagc ttgtttcaag cggaccgaat aacgaaaggg aacaaatcaa gctttcattt
     4261 atcaaattga ttacttctgc taaaaaacgt gtttggatac agacacctta ccttgttcct
     4321 gatgaaagtg tcattgctgc tttaaaaatc gcaactgcct ctggtgtaga tgtgaaaatc
     4381 atgattccca acaaaccgga tcatcctttt atttatcgag caacacaata ttatgctcgg
     4441 cagctgatca aggaaaatgt acaaatcctt gtctatgaga acggcttcct ccatgcaaaa
     4501 acattgataa tggatgatga aatctgcatg gtaggttcag caaatcaaga tattcgtagc
     4561 taccgattga attttgaaac aagtgctgtc atttacgatc ctgagttttt agaagaactt
     4621 gctactcagt tcaaagaaga tgagacacat tgttcatcca tgacaactga aacagtcaag
     4681 gaaatgtcta actggctatt attcaagcaa caaatttctc gattattttc tccaatccta
     4741 taattttttt attcaagtag agaaaaatat actaagatta aagggactgt gacaaaaatc
     4801 ttgtcacagt cccttcttcc gaa
//
LOCUS       DASJST010000171         4805 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJST010000171 DASJST010000000
VERSION     DASJST010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.173"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="U4B05_002613"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="U4B05_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN2051701.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="U4B05_002614"
     CDS             complement(2065..3063)
                     /locus_tag="U4B05_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN2051702.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="U4B05_002615"
     CDS             complement(3289..3942)
                     /locus_tag="U4B05_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN2051703.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="U4B05_002616"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="U4B05_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2051704.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1413 a         1007 c          745 g         1640 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatg
//
LOCUS       DASJST010000172         4794 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJST010000172 DASJST010000000
VERSION     DASJST010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.174"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(202..1164)
                     /locus_tag="U4B05_002617"
     CDS             complement(202..1164)
                     /locus_tag="U4B05_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2051705.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1166..2605)
                     /locus_tag="U4B05_002618"
     CDS             complement(1166..2605)
                     /locus_tag="U4B05_002618"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN2051706.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2790..4736
                     /locus_tag="U4B05_002619"
     CDS             2790..4736
                     /locus_tag="U4B05_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN2051707.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1530 a          818 c          790 g         1656 t
ORIGIN      
        1 aatttcgtaa aacaaccgta acagttgtgt aaaaatatca ttttagagca tgaaaaaagg
       61 agtggatttc tccactccaa aaaagtctaa cttttggggg tcactacaag ttgacaactc
      121 ctttttgttt acaaatcaat tggatatcta caaattgtat tttctaacga attttataga
      181 cattaaaaaa ttaatttaac gttaaatact tcctccagaa agtattgaga ttggtaaaat
      241 ataatcctta tttgtctttt cgcctttaat ttttttcaga agaatatcaa caataagact
      301 tgcaatttca tcaattggtt gtcttattgt tgtcaattgt gggaaaaatt tctcaatgaa
      361 agaggtacca tcgtatccaa taatttttaa atcttctggg atattaagtt taagttgatg
      421 agcaattttc atagtgagaa tggctgttaa atcatcagaa acaaaaatac catctggttt
      481 gtgatttata atagttgact ttatttccat ttcacgacgg atagtagaca gattattagg
      541 aattttgaaa atttttccat ttggattttg aaaagaaaaa cctagtgcac gtaattctgt
      601 gggagaatcc gtgttatcat ttcctgtaat cataatggta ttttgacaac catttttttg
      661 taaagtctta gctgcaagtt ttcccccttc aaagttatct gaagaaacaa tggaaacatt
      721 aggagctaaa tttctatcaa aagcaacaat aggtgctctc acacgctcat aatcagcaat
      781 tccaagatta tggcttgatg agattatgcc atcaacttgg ttggcttcaa gcatttcaat
      841 gtattctctc tctttagcag gatcattttg actattgcaa ataatcgttt tatatccatg
      901 tttaaataac tctatttcga gatactcaat gagttcagag taaaagatat tactaatatt
      961 aggaaaaata agaccaataa gctgaggtga ctttccttga agtccacgtg ctaaattatt
     1021 tggtttataa ccaagagttt tcatagcttc atgaactttt tgttttgttt tttctgataa
     1081 atatccctta ttattaatga cgcgtgaaac ggttgttggg ctaactccag ctaattcagc
     1141 gacatcagtt aattttgcta ccattttaat attttaattc aaagtatttt ccagtaggtt
     1201 cgcctgattt gatttgaata ccattttggt gattttcagg gaatacacga ctagtaaaca
     1261 ctttttctcc tttattaata aaaatctcaa tgattgagtt atctacaaaa atgttagcag
     1321 ttgtttcttt agtatcaatt gagcattcac gaatcgtacc aaagtcagtg gcatattgaa
     1381 tgcctgcttt gctgcgatct aaaataattt ttccttcttt ggtatctaca atcaagctaa
     1441 gtccatttcc tttactatca gagaaaagaa gtaactccga tttctgattt ggaggaaaag
     1501 ttagttcaag ctcataagta ttatttgttt ctgcttttgc tgtaaattgt tcagaactag
     1561 cacgtaatga tgtaatagct tctactggat attggtaaag tttaccatct tttattgaaa
     1621 gttcttttac aagactcatt gctccctgat aatcatactt atcagttgga taatcgatat
     1681 caggtagtcc aatccaactg acaatcagag cacgaccatc aggagtattg aatccttgag
     1741 tcgcatacgc ttcaaatcca taatcaagat tttgaatttc tgaagcgccc acaagtttta
     1801 ctttttctat gtcaagcgat tgacagatct taaaagtatt cggatagata ttcccataat
     1861 ttagctcaga tttatccaac ccttgaggac aataaatgag aacaggtttt ttgtctacaa
     1921 atactagatt tggacactca atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc
     1981 caacttcctc ccatgtttcc acgttattgt catccgcttt gtaaagtttg ataaagcctg
     2041 atttttctaa attttgtgct ccaacaatcg catacaattg tccttcataa ttaaacattt
     2101 gtggatcacg aaaatgatca gttacatcag caggtttatg aataagaaca ttctcacctt
     2161 tcgtaatgtt gtagtcttta tccatccatg ctccgatttg aagtgtatca cgttcccaat
     2221 tttcatttcg aacgtttcct gtatagagta agaatagatt cccctctatt tcatatgcag
     2281 aaccggaata agcgccgtgg ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta
     2341 catgaaaatg aactaaatca tctgattcat tgtgtaccca ttctttcaac ccatgagcag
     2401 ctccaaatgg ccaattttga taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat
     2461 taggatcgtt taacaatcct gttttaggtt cgatatgata gctagcacgc caaggtgatc
     2521 gttttacatt atccttaatt ttatccatct catctttagt ccaatcagaa taagcacgat
     2581 atcgtatttc tcgaggtaag ttcatatttt actccttcaa tttatctttt tacaactagt
     2641 atggtaaacg ttttccgttg aaaaatcaat catttcattt aaaaaaataa actatatgac
     2701 aaacgtttga catattttta agatatgcta aaataaattt gcatttcagt gaaccgtttt
     2761 cacgtaaaaa taaaaaagga gtgattcgaa tgaattacag taaagtagct tccgacgtaa
     2821 tacaagcggt cggaaaagat aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc
     2881 tcgtactaaa agatgacacg aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga
     2941 aaagcacatt taaaattgat ggacaatatc aagtgattat cggtgcaggt gatgttaatt
     3001 tcgtttatga cgaacttatc aaaaaaactg gactttcgga aatttcaaca gatgatttaa
     3061 aacaaattgc tagtaaagat aaaaaattca accccattat ggcattaatt aaattattat
     3121 cagatatttt tgttccaatt atacctgccc ttgttgcagg tggtttatta atggcattac
     3181 aaaacttttt aacagctgct ggactttttg gaccaaaatc aattgaagaa atgtatcctg
     3241 cgattgaagg tatttcctca atgatccaac tgatgtccgc tgcaccattc atgttcttac
     3301 caattttagt aggtatttca gctgctaaac gttttggagc aaaccaattt cttggtgctg
     3361 caattggtat gatcatgaca acaccagatc ttggtggctc aactgaatat tggaatattt
     3421 ttggttatca tgtttcacaa acaaactatg cttatcaagt tattccagtt ttagcagctg
     3481 tttggatatt atctatatta gaaaaattct ttcataaaaa attgccatca tctattgact
     3541 ttacattcac gccattatta tcagtaatga ttactggatt tttgactttc acagttattg
     3601 gccctgtaat gttattgttg tctaatggaa ttacagacat tattgtttgg ctatataaca
     3661 ctactggatt tattggaatg ggtatttttg gaggaacata ttcacttata gtcatgactg
     3721 gtttacatca atcatttcca gctattgaaa ctcaattact ttcagcgtgg acaaatggta
     3781 tcggacatgg agactttatc tttgtagttg catctatggc aaacgttgct caaggtgctg
     3841 caacatttgc catttggttc ttaactaaaa attctaagac aaaaagtctt gcttcctctg
     3901 cgggtgtctc agcacttctt ggaattacag agcctgcctt gttcggagtt aacttaaaat
     3961 accgctttcc attcttttgt gctttaattg gttcaggaat tgctacagca gtagcaggat
     4021 tacttaaagt agttgctgtt tcacttggtt cagcaggttt cttgggattt ctttcaatta
     4081 atgcaacatc tattccattt tacgtattgt gtgaattgat tagctttgta gttgcttttg
     4141 caattactta cttctacggt aaaacaaaag ctgctggtat ctttaaagca gaagaaattg
     4201 ctgaacaaac accagttaat tcttcaaaag tagcaacaac agatccaata tctactgatg
     4261 ttgaatcaac acaaacaaaa gtttcatcag aaacaattgt tagtcccatt tctggtaaag
     4321 tgattagtct ttcttcagta aatgatccag tcttttcatc agaagctatg ggaaaaggga
     4381 ttgccattaa accaaatgga aacacggtcc attctccagt agatggtact attcaactca
     4441 cgtttgaaac tggacatgct tatggtatta aatcaaataa tggtgctgaa gttctaattc
     4501 atattggtat tgatactgta tcaatgggag gaaaaggatt tacacaaaaa gttactacta
     4561 atcaaaaagt tagaaaaggc gatattcttg gagcatttga ccgttcaatg attgagaaag
     4621 aaggtcttga tgatacaaca atgattattg tgaccaatac ttcggattat tcagaagtaa
     4681 atatagtagc tgaaaataca gtcactgagg gtactgattt acttgaaatc aaataaatat
     4741 atttccctga gtaaagatgg aattttcttt ccaaaatcaa ataatataga ttaa
//
LOCUS       DASJST010000173         4701 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJST010000173 DASJST010000000
VERSION     DASJST010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.175"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..230)
                     /locus_tag="U4B05_002620"
     CDS             complement(<1..230)
                     /locus_tag="U4B05_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051708.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKIN"
     gene            798..2033
                     /locus_tag="U4B05_002621"
     CDS             798..2033
                     /locus_tag="U4B05_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2051709.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(3032..3292)
                     /locus_tag="U4B05_002622"
     CDS             complement(3032..3292)
                     /locus_tag="U4B05_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2051710.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(3319..4620)
                     /locus_tag="U4B05_002623"
     CDS             complement(3319..4620)
                     /locus_tag="U4B05_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2051711.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         1597 a          959 c          731 g         1414 t
ORIGIN      
        1 tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg
       61 acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt
      121 tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac
      181 ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc
      241 ttccctttaa aaaattattt ctcctttata tcatacacaa aaaaaagttc taaacaacca
      301 aaaatgttta gaacagaata aaaattttaa ttttaaaata atattcaaat taaacaactg
      361 gcgtatctgc tccttgattt ctttttgtgt tatctgaata attatcgttt acagttataa
      421 cacatagtga aaattgtcat ggcgagaaag tctaagaata tttttaaaca taactatttc
      481 tttcttacgt gtgtataatg acgacagcac acctatcaat taatataatt attttatttt
      541 tttatgttct catgtttatt tttaatgaca caacttctcg tatttatctg gcacaacata
      601 taaatcgatc aaaaaattgt tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca
      661 aaatttaaat ttagaagttg tgaaaacagc gagagacagt ttaatattca aaatatacat
      721 aggctactcg aacgctgtgt tttttagtat actagtaaga aacgaaggga tagactcatt
      781 ccaataagga ggatttcatg aagacaattc tttttgctcc tgcagtattc aatttagctg
      841 agacaacacg aatgattgag gttgccaaag ctatgaaaga ccgttattac tgtgtattct
      901 ttggcttttc taaaactttt gctcctttga tcacagaaag tggctttgag tatcatttac
      961 tagcgccgat ccttacgaaa aaacaagaag aacaaatcat aaaatttgac cagttgcgta
     1021 cacttaaaaa cccttttacc aaagaaatgg ttgcctctcg tatcactagt gaacagagtc
     1081 tgattgctaa gtttgagcct cgactgattg tgacaggttc gaatgtgaca atttttttat
     1141 cggcccgcaa taaaaagatt cctttagtat atgttaaacc ctatgctttg agtgaacctt
     1201 tttttcaatc agagcatgcg ctgatgcccc acgagttacg tttcttcggc cctttaagca
     1261 atcccctttg gcactggaca aaaaagcgta tagtaaatac ccgatggatc cccaatgcct
     1321 tcaaacacgc tgcacaaaaa gaaatgcttc ctttaccaaa aacaagttta caaatgatgg
     1381 atgctgatct aaatctcatc acaacgccta aactattttt ggaagaagaa cagctgccgg
     1441 aaaattatcg aatcgttggt ccaatttttg ctaagctcag tacttccttg cctgaagaag
     1501 tgctaacttt tatacagcga aagcaacaag aaagaaaacg tattatctat ttcgcaatgg
     1561 gtagttcagg caatccgcgt atgttacgca aaattttgtt cttcttacaa aaacaaccgg
     1621 atctagcggt tattgcacct attgactatc tgcttgatga cattcaatct gactcagaag
     1681 atcttttaat aacagaatat ctgtctagtc atcttttgca tgaattcatt gatttttctt
     1741 ttatccacgg cggggaaggg accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg
     1801 gtgtggggat gcaatttgag caaagctgta atattgacta ttgtgtaaaa tacggaaacg
     1861 caatcgccct cacaaaacca gtgactgaaa gaaaactgca gattgctctg cgcgaagttt
     1921 cttcacccaa gattcgtcag caggcacgca cgctgcaagc cgtttttcca ataaatggtc
     1981 cacaacaagc aattcaagaa atcgaaacct tcctgagcaa ccacccaata tagtttcaag
     2041 tagaaaaagc ttttttaata gagactcatc agtaatttcg ctattcgaga cccagacagt
     2101 gaaaatacaa aaaaagtaaa tagtggattg aaaagttaga gttacttaaa ttacggtgtc
     2161 taaaaaacac aaaatcatcc ttaaaataaa agatctgacg ctatccaaat gagcataggt
     2221 agcgtctgcc atttattctt agtcaaattc tttgacacct ttctctggtt ggatcatctc
     2281 aacgtttctt caaaagaaag accataataa ttaggcaaat acattttctt aaaatgtgtc
     2341 atatagatct agaagcatcg ctttgtagag atttgtgccg ctagtcccct caacaattac
     2401 taaatcaaat gtaggattta ccactttatc tttgataatc aacctccctc ttctgttgag
     2461 acaatgatct cgcaatccga tgcatactcc tatataaact gccagcaatc cccacatatt
     2521 gagtagatca tgtcagaatg ttctatgtcg tcagcaacga tacactaaaa gtcacgttca
     2581 ccatgactga ttgctattcc cagcacaatt atttctatac acgtttcatg atttgaataa
     2641 acactcacat catacatatt accattcata caccatactg cagcccctac tatatggttg
     2701 taagctccca aatctatatt tgacttgatc actttggtag tagatctact aattctaaat
     2761 ccattgctga taaaatttcc attttttcct ccttctttct gccaaaacag ttaaaacaat
     2821 aataatcttt atgtaaaaaa gggctgtgac aaaagccttg tcacagtccc atattccgaa
     2881 taaacggtgg tcaaaagctt gcatctgcgg taataagttc aaccaaacga aaaataagcc
     2941 aacatttcac aaaaatttaa gaagcaattt tcgtaaattt cttcttattt cttgaagcta
     3001 accacttctg ttacaaccac ttttagaaca tttaaagctt cgatgtgaca aaccatctat
     3061 gctgctcgat cataaaattg atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa
     3121 acaaaacatt catatgcggt acaatgatct ttggaagaat cacgatcaaa aatgatgcca
     3181 gtgcccaaat ccatcgattg atttgaatat gatatgtagc tagcatattc aatataaggt
     3241 gactagccgt taataaaaag ataaaaacaa aaaataaaat cagccaatac attgtttccc
     3301 accttctaaa aaccaaactt agacagtctg gacttcgttt tctccacgac tttgttcatc
     3361 tgacagcatc tttttatcat aagcttttac aaatggataa tagataataa atgctatcaa
     3421 tgtgttgatc atgatcaaga taacaacacg ccagtcattt cctgctgaaa gataaccgcc
     3481 gattggagct ggtaatgtcc atgttggcaa aacagtaaat ctattgacca tttctaattt
     3541 gactgcaaaa taactgataa tagtcaaaac taaaggaact aagttgaacg ggatgaacat
     3601 gtaaggattc aacatcatag gagctccaaa aattatgggc tcattgatat tgaagatacc
     3661 aggaataata gaaaaacgac caacctcttt caagaatttt gatttacaga agaatataaa
     3721 caaaacagat accacgatcg ttgctcctgc accaccaatt gttaccgtcc attgataaaa
     3781 ctgttcagga gcgatatagg gtaattgggt ggcagctgta ccatctgcca atgcctttgc
     3841 attctcatcc aacatgatca gccacattgg acgaacaaca gcccctacaa tactcatgcc
     3901 atggatacca aatgaccaca acaaatggat caaaaacatt ggtagtaaaa caccaagcaa
     3961 attattccct aaaatattgg ttaaaggctc gaataacgat aataaggcag cgtttacatc
     4021 aaagcccgcg acgcttcgaa tcagccaagt aattgttaaa atgactgcac ctggaatcaa
     4081 tgcctcaaaa ctacgggcaa cagcaggtgg cacttgttct ggcattttga tagtaatatt
     4141 tcgattttta aagaaacgat atacttccac tgcaaaaatc atagataaga tccccgcaaa
     4201 catcccagat gctcccaaag aactcatagg taagacccag ccaagaggtg cctcttcagg
     4261 agttaaaacg tttacaggaa cttgtaacat aaagaacgtt cctaaagata atataccacc
     4321 tgtcacacta tctaatccat atgatttagc tagatccgaa cccataacaa aagttgcata
     4381 aatagccatc aatcccatcg tcatcctaaa gggtaaaaca atcgttgccg aataaggggc
     4441 aataaattca ttccatccag gaaacggtaa attgataaag accataaaaa atgtcccaat
     4501 caaaatcaat gacaatgttg aaacaacccc attacgaatt gctaataaat gacgctgtgt
     4561 accaattttt gccattggtg ggacaagttt ctcttcgacc catttcatca gcttttccac
     4621 taaaaccact ccttttcaat aaaatatgaa ccattcattg catgacaacg caaagaatgt
     4681 tcataactaa ttatataatt a
//
LOCUS       DASJST010000174         4636 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJST010000174 DASJST010000000
VERSION     DASJST010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.176"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..482)
                     /locus_tag="U4B05_002624"
     CDS             complement(12..482)
                     /locus_tag="U4B05_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN2051712.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="U4B05_002625"
     CDS             complement(641..1099)
                     /locus_tag="U4B05_002625"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN2051713.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="U4B05_002626"
     CDS             complement(1270..2415)
                     /locus_tag="U4B05_002626"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEN2051714.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="U4B05_002627"
     CDS             complement(2428..3345)
                     /locus_tag="U4B05_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN2051715.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="U4B05_002628"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="U4B05_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN2051716.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="U4B05_002629"
     CDS             4437..4607
                     /locus_tag="U4B05_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051717.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1448 a          844 c          875 g         1469 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtata
//
LOCUS       DASJST010000175         4590 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJST010000175 DASJST010000000
VERSION     DASJST010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.177"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            186..2855
                     /locus_tag="U4B05_002630"
     CDS             186..2855
                     /locus_tag="U4B05_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN2051718.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="U4B05_002631"
     CDS             complement(2946..3566)
                     /locus_tag="U4B05_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2051719.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="U4B05_002632"
     CDS             3680..4219
                     /locus_tag="U4B05_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051720.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="U4B05_002633"
     CDS             4216..4554
                     /locus_tag="U4B05_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HEN2051721.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          790 c          860 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DASJST010000176         4557 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJST010000176 DASJST010000000
VERSION     DASJST010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.178"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1016..1306)
                     /locus_tag="U4B05_002634"
     CDS             complement(1016..1306)
                     /locus_tag="U4B05_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051722.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="U4B05_002635"
     CDS             1708..1944
                     /locus_tag="U4B05_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051723.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="U4B05_002636"
     CDS             1951..3903
                     /locus_tag="U4B05_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2051724.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="U4B05_002637"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="U4B05_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN2051725.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1557 a          803 c          753 g         1444 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagtt
//
LOCUS       DASJST010000177         4528 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJST010000177 DASJST010000000
VERSION     DASJST010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.179"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..1261)
                     /locus_tag="U4B05_002638"
     CDS             complement(29..1261)
                     /locus_tag="U4B05_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN2051726.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1515..2084
                     /locus_tag="U4B05_002639"
     CDS             1515..2084
                     /locus_tag="U4B05_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2051727.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2262..2702)
                     /locus_tag="U4B05_002640"
     CDS             complement(2262..2702)
                     /locus_tag="U4B05_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN2051728.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2860..3624
                     /gene="map"
                     /locus_tag="U4B05_002641"
     CDS             2860..3624
                     /gene="map"
                     /locus_tag="U4B05_002641"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN2051729.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3656..>4528
                     /locus_tag="U4B05_002642"
     CDS             3656..>4528
                     /locus_tag="U4B05_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN2051730.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPS"
BASE COUNT         1354 a          849 c          902 g         1423 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaact aattttgcat
     1441 atcaaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa
     1501 ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg
     1561 cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca
     1621 aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac
     1681 aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata
     1741 cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga
     1801 agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct
     1861 cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag
     1921 agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga
     1981 tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc caagacagta
     2041 tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc
     2101 cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag
     2161 cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata
     2221 cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt
     2281 tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta
     2341 acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc
     2401 gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc
     2461 aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca
     2521 tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct
     2581 acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca
     2641 atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc
     2701 atgatgtgcc tcctcgttta taattattta acaattatga attataccaa aacatcattg
     2761 aaaagaaaat aaaacctaga gacacctggg gaatgtcatt tggctaaatg tatgccgcta
     2821 tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca
     2881 cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg
     2941 cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat
     3001 atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact
     3061 tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac
     3121 ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt
     3181 tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag
     3241 aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt
     3301 catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt
     3361 catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga
     3421 aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga
     3481 acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt
     3541 tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca
     3601 caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa
     3661 agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa
     3721 gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt
     3781 gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca
     3841 agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg
     3901 tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt
     3961 agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc
     4021 gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat
     4081 tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat
     4141 tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac
     4201 tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat
     4261 tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt
     4321 cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat
     4381 cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc
     4441 tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca
     4501 tcaaatcaaa aaacgtcgag atccaagc
//
LOCUS       DASJST010000178         4515 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJST010000178 DASJST010000000
VERSION     DASJST010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.180"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(460..885)
                     /locus_tag="U4B05_002643"
     CDS             complement(460..885)
                     /locus_tag="U4B05_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051731.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1259..2230
                     /locus_tag="U4B05_002644"
     CDS             1259..2230
                     /locus_tag="U4B05_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2051732.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2262..3659
                     /locus_tag="U4B05_002645"
     CDS             2262..3659
                     /locus_tag="U4B05_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2051733.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3818..4441
                     /locus_tag="U4B05_002646"
     CDS             3818..4441
                     /locus_tag="U4B05_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051734.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1581 a          725 c          919 g         1290 t
ORIGIN      
        1 aaattactac gtgatgtaga ataagccttt ctatcgtttg gttattttat attcaaatat
       61 ttgaaataaa taaatcttta ctctttgatt tgtaaactga aatgatccac tgtttgtttt
      121 actgatgaag cttgatcaaa ggaagaagct gagtttgaag ttcataaaaa tcagccatag
      181 tgagttttgc ttggatttgt ttatcttctt ctacttttta cataattttg aacaggataa
      241 gattcaaatg ttttaaatga atctttgact agtttgtgac agttgtcgca tatgctcaag
      301 cttccttggt cattaaattc tgtatggata tttttgttgt tattacatgt acaataatca
      361 ggactatcga tcatccattt cgtctccttt acaatcatct tgcttcttta ctatagcaga
      421 aaagaagggg agaaacaaaa gaagatccaa cagacggcac tatagactgt agatgttgcc
      481 atcctcatcc ttcaaacggt aattaccgat ttttgtaaat atgactgttg tttctttttt
      541 attttctttt cctgaaataa tatattcgtc atctttggta gtgatcttga tatctgtcag
      601 cactgttttt tcttcagatg attcagaaga atcagttcct tgtgtctgat acgtgcgtgt
      661 ccattccatt tttttatctg taaatgtcaa ctgtcccgcg gataaatcca tatctttacg
      721 tacttttagc gtatatacac tgttggtggt atctgctttt tccgctttta agacagaaga
      781 atcggttgtt tcgtccgagg attggtcaga gccgccaaac aaatcacagc cagtcaatgt
      841 caaaagtccc gcagacaaaa taaatagtac gattaatttc ttcaaaatac ttcccccatt
      901 tgataggata aagaaattat accattggaa tgtaaaactt caaacggatg gaagagaaaa
      961 acgcacaaaa cacccacaaa tagagtaaat tttagaataa aattttaaaa aggcaaaaaa
     1021 tccgccactt tcagaaagcg ttttaaaaaa gtgttgcaat cttttaaaaa aatgctatat
     1081 taaaaatgaa ccggttcaaa tacttgttta agtataatta atagtgagag gaagttgctc
     1141 tactgaagac tttagttata tggatattga tgacaagcaa cactatgcaa agaatgcggt
     1201 catcataatc gaacaacaag aaagagaaat caaatgtaac tggcaggagt gagaatgtat
     1261 ggcatcgatc cgtgatgtgg ctaagatggc tggtgtttca gtgggaacag tttcccgtta
     1321 cttgaatggt cagcaactga aggaaaagaa catgaaaaaa atagcagaag cgatttcagc
     1381 gttggactat aaagaaaaca tcattgctaa agggttgaag aataatcgaa gcttctctat
     1441 tggacttttg ataaacggga tttccagccg ttttgggtcg gaagttgtct cagggatcga
     1501 aagagtagct gaagaaaatg ggtatagctt attattaagc gggtttgccg atcagccaga
     1561 aaaaattgaa caaaaaattg aatacctgat gaagcatgtg atcgatgggc tgattgtctt
     1621 cttatccgaa gaagagtggt ctggatttga aatgctcaca aaaatgagta ttccagttat
     1681 cgcattgaat tgtccagaag gaccagcagg tattgataca atattagtga atgatcgaga
     1741 aagtgtagca aaagtgatcg ctcatcatgg tgaacaagga cataaaaaaa tcggatttat
     1801 tgctgccact caaacagact acgtagcaag agaacgttta gcaggagcca aagaagcggt
     1861 ccaaaatgat ccatcgatcc aattagaggt ctttacagga gactattcta gaaaaagcgg
     1921 ctattacggg gcaaaagcat tattagaaaa aggggcaact gctatttttg tcagcaatta
     1981 caatatgtcg attggtgcca tcgaatgttt caatcaagaa ggtgtcagaa tcgggaagga
     2041 tattgcgttc agtcactatg attatttaga taaaaacgat ctgagtatcc ttccgaaaat
     2101 cacgatcacg ccaccaacag ataaaatcgg ggaacgagca gcagaacagc tgttaaaacg
     2161 aatcaaaaaa gaagaggaag aaaatgaaaa agtgattgtg atggaaaata tcatacatgg
     2221 aatcaactaa aacgaactaa aaaagaaaaa gaggtgcttt gatgaaaaaa tatcaatttc
     2281 cagcaaattt ttggtggggt tctgcagcaa gtggcccaca aacagaagga aggatttcag
     2341 gcgatggaaa aggagagaac atttttgatt attggtacaa aaaagaaccg caaaagttct
     2401 ttgatcaagt aggacctgaa aaaacctcgc aagtttatac gaagtatcaa gaggatgtac
     2461 agttgatgaa acagactggg cataattcat ttcgaacatc tatccaatgg agccgtttga
     2521 tacctgatga atctggaaca atcaacgaag aagccgttcg tttctatcat cgttattttg
     2581 atgagttgat tgaaaacggt atcgaaccct ttgtgaatct gtatcatttc gatatgccga
     2641 tgtatctgca agaaaaaggc ggatggttga accgagaaac ggttgaagcc tatgaaaaat
     2701 atgccaaaag ttgttttgaa ttgtttggtt cgaaagtaaa aaaatggttt actcataatg
     2761 aaccaatcgt tccagtcgaa gcaggttatt tatatcgatg gcattatcca gaagagtcag
     2821 atatgaaaaa agcgatccaa gttggctatc atgaagcgtt ggcttcagcg ttagccatca
     2881 aatcttacca tgaaatggag caaggtggtg ttattggtat catcttgaat ctgacaccaa
     2941 gctatccaag agacgaatca aaccctgaag atgttaaagc agcgcaaata gcagatgcgt
     3001 tcttcaatcg ttcattcctt gatccagctg taaaaggcga gtttccaagt gaattgattg
     3061 agatagtaaa agaacttgat ctcatgcctg tgatccatgc agaggatttg acggttatca
     3121 aagaaaatac agtagatctt ttaggtatca attattacca gccacgccgg gtaaaagcaa
     3181 aagaaacacc aatcgatact aaaaacggtc caatgcctga ggattatttc gacaattatg
     3241 aaatgcctgg tcgtaaaatg aatccttatc gcggatggga gatctatgaa caaggaatct
     3301 atgatatttt gatcaatgta cgagatcatt atggaaatat cgactgcttt atttctgaaa
     3361 acggtatggg tgtagaaaac gaagcacgat ttatcaaaga agatggcatg atccacgatg
     3421 attatcggat cgaatttgtc caatctcatc tgaaatatgt ccatcaagcc attcaagaag
     3481 gtgccaattg tttaggctat catatgtgga catgtatgga taactggtcg tggaccaatg
     3541 catataagaa tcgatatggc tttatttcag tagacttagc acaagatgga aaaagaacaa
     3601 tcaaaaaatc gggtcattgg ttcaaagacg tatcagataa aaacggattt gaggcttaag
     3661 aaagaatagt tgagagcaaa tgcgtgaaat tctccattct taccattttg tgaaaaagaa
     3721 gttcaattat ttacgatctt tttcaacttt tttgtctttc tatttcattt tttgtatata
     3781 atagaatagt caaaattaaa taggagtgga ggaaagcatg aggaaaaaaa gaccgtttct
     3841 tctcttttgg ttattcggtt ttttcttact gcttatttta tccgcagcat tttcaggaaa
     3901 aatgacagca gatcttgctc agaaagaaac agaaaaaatc gtagcaagca aagaatcagt
     3961 agataatttc aaaaaagaat tctcaactta ttggtgggcg gagtataaaa taactgccaa
     4021 ttacgaaaaa ggcgaattgg acatttattt gccaaaagga aaagaagaaa atcaatttat
     4081 taatatcgat gacgtactta gtgcgatttc tctcagacaa tatcaattta aagacaagca
     4141 tttgaccgtt ttgcttttga ctcatgatgg cgacaaagta gcccgtttga aaaatggaaa
     4201 agtgatcacc gcttattctg aaaaagtaga caaaaaagtg gtcgccgatc gtttagcgga
     4261 atggaaaaag acaaatgaac aagtcaagaa aacaactgtc tcttctcaaa aaaacgagtc
     4321 gacaggaaca agtccttcag attctggcag tggacaaacg ataacgggaa gtgaagaaaa
     4381 tcaagcctca attattacga atgagtcatc acaaggacaa tatcaatcct atggacaatg
     4441 aaaaacagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggcaaaa
     4501 tagttcctca tattt
//
LOCUS       DASJST010000179         4483 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJST010000179 DASJST010000000
VERSION     DASJST010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.181"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..443)
                     /locus_tag="U4B05_002647"
     CDS             complement(<1..443)
                     /locus_tag="U4B05_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051735.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSR"
     gene            complement(651..1577)
                     /gene="glsA"
                     /locus_tag="U4B05_002648"
     CDS             complement(651..1577)
                     /gene="glsA"
                     /locus_tag="U4B05_002648"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN2051736.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1600..3027)
                     /locus_tag="U4B05_002649"
     CDS             complement(1600..3027)
                     /locus_tag="U4B05_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2051737.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3058..4272)
                     /locus_tag="U4B05_002650"
     CDS             complement(3058..4272)
                     /locus_tag="U4B05_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN2051738.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1410 a          942 c          787 g         1344 t
ORIGIN      
        1 cgcgagtcaa attctgggat aatgatttct ttatttttca aataatcatt ttctttaaaa
       61 aaatccaata cgattttttt gattgtatat actttgctca aagaaataaa ttcaagatca
      121 gccagttctg tactcgtgaa tataccatta aaataataac ttaaaaactt taaaaaataa
      181 gattgaccgt acagaaactg tgtgagctca gaaagagcaa taccatgttt gaatacgagt
      241 ttatacttat gcttctctct tacttgttct tttatacaat attctagaca ctcggctaga
      301 ctttctagat cgtgaaggat cgttggtgta gtcaccctta gagaaatagc tgtttgttcg
      361 atgtctattt cttggaattc aaataattgt tcacacagat acacttttct aaatatattt
      421 cgctcgatgt aattttccag cattcctgat acacgcctcc agtttttctt atgattttat
      481 ttaatcatat ctgtaaatcg tatttttgtt tcctttttat ttagaaaagc cctttcaaaa
      541 accaataaaa aaagaaactg ggacaaaagt caaacaagct cacaatttca gcctcattgt
      601 aggtagcttt gagccgtttg gacttttacc ccagcctctt tcaagaatgg ttattcaaag
      661 atgtcagcgt ctagttcttt gactacatct ttcaacaatt ggataccagc tgcgctgtta
      721 ccaaatggat ctagtgccgg gctgaagaca cctatgccgt aacgatttgg cacagctgcc
      781 atcaacccgc cgcctacacc gctttttgca ggtacaccaa catggacgga aaactctcct
      841 gattcatcat acaaaccagc agttgtcata attgatttga cgattgtagc actttcttca
      901 gtaatgattt gtttgccatt ccaaggagca attcctttat ttgccagcac agcagctaat
      961 tttgctatgc ctgtagcggt gacattgagg gagcattgtc tgaaataagc atcgagtatc
     1021 tccggtacgt ctccttcgat catttgatta cctttcatat agtaagcaag agagcgattg
     1081 atatcacctg ttcttgattc agaatgatag atttcttcat tcaatgtgat attaggatca
     1141 tcacagatct ctttcataaa tgacaagatt cgctcaaatt tttcatctgc atctttaccg
     1201 gcaatcaacg aggttgttgc aatcgctcct gcattaacaa aaggattcat tggatgtttg
     1261 cgatgattga tttccatatt catgatagaa ttgaaggcaa aaccggttgg ttccgtgttg
     1321 attgtttgaa agactttttc tattccgttg tctttgattg ctaataacaa tacgggaact
     1381 tttgacatgc tttcgatggc aaagcgcaca tcggcgtctc ctccctcaat cagttgtttt
     1441 gtatccaaat cataaatcgc catacctaat tgtttgggat ccacttcagc taatgctgga
     1501 atgtaatccg ccacttgtcc tgtttgataa tagtggaaat tttttccaat caattgttct
     1561 aattctttta cttccatttg aataaccagc tttcattttt tatttttctg catgctggaa
     1621 tttatgacgg catgcgtaga tcgtaaatgg aataactaag acgataagat aagaaacaat
     1681 cagaatcgtt tcataaatcc catgctgtcc agctgcgata ctagcaggtg gaacaaatga
     1741 gatacaaaaa gcaaaaagtg aagtcaaaag acctaaacaa gcaacgatta cttttacaac
     1801 ttttccgcct ggaacctgat aggctctttc aagactgtca tctttaaaaa ccagcttcaa
     1861 atagcccgcg aaaaacataa agtacaacat caggtagatt acaacagtca atgacatcgc
     1921 tgcaagaaat gaaagattat tccccccgcc gcttaatgta aagacggctg cccagattgt
     1981 tgcaacgatc ccatttgcga tcatcaaatg agcaggaaca ccgtgtgtgt tttcttttgc
     2041 aaaatattca ggaagtaatc cttcttctgc tgctacgtga agtgcttttg aaggagaggt
     2101 tacccaagaa gcaatttctc cgatgacacc taaacaaaca agaatcgcaa gaattttcgc
     2161 aatccatgca agatttggat caatgtgtct taataggaat tctaacgatt gaatcacccc
     2221 tgtattaaga gacaaaccat gttgaggtac tgtagtagcg acggtcaatc cgccaaaagt
     2281 atctaaaatg attgccagaa taaccaatac aaacattgct aatggataat cctttttagg
     2341 gttttccatt tcatttgcat gactggcaga tgtttctacg cccatatatg ctaaaataaa
     2401 ggaaacaaaa acaaccagcg taggcaattt cgaaaaatca ggaatcaatg ttttcataga
     2461 cagtatcgtt tgtagtggag cgcctgaagc gacataatgg atccctaagc caaacaagac
     2521 taatgctggc aacaagatac cagctacaaa accgatccga gcaatcaatg aagtgtattt
     2581 ggttcctttt aactgtgaga gtaaaatagc ccagaaaata atcaatagga ttgccaattt
     2641 cagatattta ttgttttgta tagcaccaat acccgttgca taggataatg caccagttaa
     2701 gaaatacagc atggtcaagt agccaactgt tatctcaaac cattggaaaa agattgcggc
     2761 aaaaccgaat cgttttccca atgtgcgact gacccaagta tagacaccgc ctttttccca
     2821 tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc atagcaaacc
     2881 gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg gatattcata
     2941 taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta atttttttgc
     3001 aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga atttgtttca
     3061 attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt cttttgtagt
     3121 agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca cataggcata
     3181 cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc tgcttggatt
     3241 cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg tgaatccgcc
     3301 catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt tacgaaaatc
     3361 aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac tttttgcttc
     3421 tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg ttagtacgtc
     3481 gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa tcatcgcaaa
     3541 tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg ctcgaagtgc
     3601 accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga tagcagctgc
     3661 cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac caagtgtgag
     3721 gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac cagcgaaatc
     3781 tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa cagggaaata
     3841 aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca atctccctgc
     3901 aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt aaagaaagaa
     3961 cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa catgtctcat
     4021 caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa aggccaatcc
     4081 gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag attgagacat
     4141 gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta gaccaggagt
     4201 catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg tattgatagg
     4261 taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga aggtgtagtt
     4321 gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag acaattaata
     4381 aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa aaaataaacg
     4441 gtcttctttt tatagaaaac cgtttatttt cgtctttttt tat
//
LOCUS       DASJST010000180         4477 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJST010000180 DASJST010000000
VERSION     DASJST010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.182"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(832..1125)
                     /locus_tag="U4B05_002651"
     CDS             complement(832..1125)
                     /locus_tag="U4B05_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN2051739.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1528..2706)
                     /locus_tag="U4B05_002652"
     CDS             complement(1528..2706)
                     /locus_tag="U4B05_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2051740.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="U4B05_002653"
     CDS             complement(2810..3124)
                     /locus_tag="U4B05_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051741.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="U4B05_002654"
     CDS             3231..3446
                     /locus_tag="U4B05_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051742.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="U4B05_002655"
     CDS             complement(3746..3988)
                     /locus_tag="U4B05_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051743.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="U4B05_002656"
     CDS             complement(3975..4403)
                     /locus_tag="U4B05_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2051744.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DASJST010000181         4449 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.183, whole genome shotgun sequence.
ACCESSION   DASJST010000181 DASJST010000000
VERSION     DASJST010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.183"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..266
                     /locus_tag="U4B05_002657"
     CDS             <1..266
                     /locus_tag="U4B05_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2051745.1"
                     /translation="VVFDDIGAELGSGVSNSRQFTNNTLNTLLEARQNKATIITTNLS
                     GPELREAYGERIVSRIFKNSEGYALKFQQTADKRIKPVKGSIA"
     gene            263..622
                     /locus_tag="U4B05_002658"
     CDS             263..622
                     /locus_tag="U4B05_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN2051746.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            637..798
                     /locus_tag="U4B05_002659"
     CDS             637..798
                     /locus_tag="U4B05_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2051747.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            801..1100
                     /locus_tag="U4B05_002660"
     CDS             801..1100
                     /locus_tag="U4B05_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051748.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            1100..1414
                     /locus_tag="U4B05_002661"
     CDS             1100..1414
                     /locus_tag="U4B05_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEN2051749.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            1591..1902
                     /locus_tag="U4B05_002662"
     CDS             1591..1902
                     /locus_tag="U4B05_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2051750.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            1899..2117
                     /locus_tag="U4B05_002663"
     CDS             1899..2117
                     /locus_tag="U4B05_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HEN2051751.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            2130..2411
                     /locus_tag="U4B05_002664"
     CDS             2130..2411
                     /locus_tag="U4B05_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051752.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            2408..2734
                     /locus_tag="U4B05_002665"
     CDS             2408..2734
                     /locus_tag="U4B05_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051753.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            2731..2928
                     /locus_tag="U4B05_002666"
     CDS             2731..2928
                     /locus_tag="U4B05_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051754.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            2925..3134
                     /locus_tag="U4B05_002667"
     CDS             2925..3134
                     /locus_tag="U4B05_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051755.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            3131..3418
                     /locus_tag="U4B05_002668"
     CDS             3131..3418
                     /locus_tag="U4B05_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051756.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            4010..>4449
                     /locus_tag="U4B05_002669"
     CDS             4010..>4449
                     /locus_tag="U4B05_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN2051757.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFAS"
BASE COUNT         1646 a          670 c          973 g         1160 t
ORIGIN      
        1 tggtggtttt tgacgatatt ggagctgaat taggtagcgg ggtatcaaat agtaggcagt
       61 ttacaaacaa cacgttaaac acgctcttag aagccagaca gaacaaggca acgatcatca
      121 caacaaactt atccggtcct gaactaagag aagcctacgg cgaaagaatt gtttctagaa
      181 tatttaagaa ttcagaaggt tatgcgctga aattccaaca aacagcagac aagcgcataa
      241 aaccagtgaa aggtagtatc gcatgaataa ataccgtaat aagaaaactg ttcatcgagg
      301 tatcaagttt gattctatcg cggaagcaga gtattacgat ttagccttgt ggcaagcaga
      361 agcgaacggc tggaaagtaa aacttcagga aagatttgag ctgatgccga aatttgaact
      421 agatggaaaa aagtatcgca agatcgagta tattcccgac ttcacatttt ataaaaacgg
      481 caaacttgtc aaagtcgtag atgtcaaagg aatgcagaca aaagacttta agatcaaggc
      541 aaagttgttc tgtcatcaat atcaagtgcc gttgatatta gctaaaaaat atcggaatac
      601 gttcaaggaa gagcgttttt aacgaggtgg tccatcatga caacagaaga agtgattcaa
      661 atgcgtattc gaagccttca acgtgagatt gacgatctgg aacggataaa ggcagtgatg
      721 gtcaatgaaa cggcgagaaa ggcaatcaat ttgcacatag tgaatttaag aagggaaatt
      781 cgtagattgg aggaatgagc gtgggtaaga aagcagcaat gaaacgaatc attgaactga
      841 cacattctga gaattggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta
      901 aatcaatgtg gactgaaaag cctaaacgga aaacgccgag aaaaattgca atctggcatg
      961 gtgatcgaat tctagtaacg ggtacagctg aacagttatc tgaaattact ggactgagca
     1021 aaaacatcat ctgggataga gctaggagct tatggattga ttcaaagggg cgacagttta
     1081 ggtatgtgga ggagaaataa tggatctcat tacacaatac agtgatatca tcctcaagaa
     1141 aatcatgatg aagattcaga aagataaaaa agcaaaagaa cgagcggaat tagttaagtt
     1201 ggaaatggct gaaacaggag caggagtgcg aagtagcagg cattggaaag cagcagcaaa
     1261 cattgaattt tattacaacg aaattcaaaa agggttcgat cagatgcgtg agctggatcg
     1321 gcaaacaaat tggagcaaga aacttcatca ggatcgtttc aaatttgtag aaaagtatag
     1381 agagatactg aatgagtatt tcgaggagga ctagatgttt aattcaacgt tagcgtcaat
     1441 tcacttttta ttctatataa cgatatttgt agctatatta attaatgcga taaaaagcgg
     1501 ttttttaaca ccaatcggca caattttaac attagcttca tttgttacaa gttgtattat
     1561 ccaacttaaa tatataaagg agaatgaata atggacgaac taatcacaaa agtagagcag
     1621 tgggctaaag ataagggatt ggatcaagct gattccagca agcaaatgtt aaaaacgatc
     1681 gaagagatcg gggaagttgc cgcttctcta gctagaaaag atgaacatgg tttaagagac
     1741 ggaattggag atgtagtagt aaccttgatt attttagcta tgcaaaatga tatggatttg
     1801 tacgagtgtc tgaaccaagc atacaacgaa atcaaaggac gcacaggaaa aatggtagat
     1861 ggtgtattcg tgaagtcgag tgatttggag gacagcaaat gatcagccta acaatatttt
     1921 ggtgtgtaat tggcggctta ctagcgatgt taggagcata cctcatttac gagagtgcaa
     1981 aaggcaaaaa atatacagca gtagggatta ttttgtttgt tgtcggaatt ttagttgcga
     2041 ttggtgcaga aataaatctt tatttaaacg gtacgacaga agagttgatg tggtttttat
     2101 tgtggacaag gagataggag gacagcgaaa tgggcgatat gggcgaatac tggaaagata
     2161 ttaaaccgca tctaaaagaa cgtagaaaac aacatgtcag aaagatgggc aattcagcaa
     2221 ctaaaaatat tgaaaccttg ggatttgaat ttactcatta tccaaacaat catcaatttg
     2281 caattaatac ccataatgga attattgact actggggaac tacaggaacg tggattgagc
     2341 gcaaaacaaa aaaacgtggt aaagggatac gtagtttgag gaaatatatt aatcaggagg
     2401 acagcaaatg aactatacac aacaagaact gactgattta tgtccaaaac atgtagctga
     2461 gttcatcaat aatgaagtcc tgcctaaata tgcagatggt ttaaatacag cagaaaacgt
     2521 tacagatttt atgattaatg atgctattga tcgtttgata tttttagaaa tagattgtat
     2581 tgcttattat cgtctacacg cagaagttgc tttgattgat ccgtatattg ctttaagcca
     2641 aaatcgaaaa atactcgtag cctatattga taccaaacag attttagaag gcagagatgt
     2701 cgagatagag atcgatgagg aggaagtggg atgaaagcat acacaatcaa gttttatgga
     2761 aataaaacct atgtatacat cctaagcggt ctcagacttg attttagagg ctattcaaaa
     2821 gaagaccttt tagaatcctt atatggttac ggatatttaa atgaagatga actaataagc
     2881 ttagagaaat ttacaaatgc ttggacgatt gaggaggaag cagaatgaag atttatgtag
     2941 taaagttcgg gaataaattt tatagaagtg atgaacgctc tataggggct aacacattat
     3001 tcattgtaga catactccaa agtgcgagat ggtttgatag cctcgaagaa gctaaccaag
     3061 tttcacgacg acttggtgga ttaacacaag tatatgaact ggtcactgtc gatcatgagg
     3121 aggtagaaag atgaaattaa aagacggatt ttacgctagt agtcatggta tcggcggttt
     3181 aatgctagat atgccgacaa agaatcctaa aaaacgtaag aaaccaaaat tcaaagtcgg
     3241 tgacatggtt cgctgcgaag cagaagggtt catctatcca tttcgtggat atgtagaaca
     3301 cgtctataat cactcagcga tcattcgtat tgaaaacacg atggaatgtg ataagtggtt
     3361 agcgaaaagc aaagagaatt tagctgtagc gagattggtg gatatgaagg ctatataaac
     3421 agcgaaaaaa tgtacttcta agagaaaaat ttatttttta taaaaatagg aaagtatcat
     3481 tatgaaaaag accacagaga taaataaacc taccacataa aaaatgaata tttcttgcga
     3541 ttcaatatag gtatatttaa caatttgtac aataaaaaat actataggta aaaaagataa
     3601 catgaggagc ttatttgttc tagagtgttt gacaaagtaa caaaatgcga cgataccaat
     3661 acaaacggga atccaaaata acagcatgct ccacatagtc actcaaccat ctcctatacg
     3721 ctttattatc aatagataat atcatttttc gtaataaaga taaaaaaata agtaaggctt
     3781 ctcattaaaa tataaaaaag acagccgacc actggctgtc cttagcagaa tattgaaata
     3841 gtgagccatc tgttttccgc cagatagctc aacatgtata tgggtaacga gtgcaacctc
     3901 gctacttgaa aagcttagca taagcatggg tataaaaaaa gccggattcc tccgacctta
     3961 attaataatt ctgacacaat tattatatca taaatggagg aatcaatgga tggtactttt
     4021 cgatgtaaag aagtatgaga caccagaggc aaaagatgtg gatatggatc gcacaaaaca
     4081 taatgttggt gtttttcttt cagcatatct gtcagctaga tgtaggatag gtcaacctcg
     4141 ggagcctaaa gtaacggcat cttactcctt ggttccacct tctacagcaa ataatacatt
     4201 tgaagcagaa cgaatgatga ttgagagaga ggaagcgcaa gaagaatttg agtacttgca
     4261 caaattattt gttcgtggct attcagcaat tcagcatccg cataaacctg atgtaacaga
     4321 aaggcgcaag aagatattct atgatcgtta tatcaatggt ctgcccattt atgtaactgc
     4381 tcaaaggaat aatactagcg aagaatcggt taaagtagaa tcaaacagaa ttatcatcca
     4441 atttgcttc
//
LOCUS       DASJST010000182         4448 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJST010000182 DASJST010000000
VERSION     DASJST010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.184"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..898
                     /gene="vanY"
                     /locus_tag="U4B05_002670"
     CDS             <1..898
                     /gene="vanY"
                     /locus_tag="U4B05_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEN2051758.1"
                     /translation="FLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLENSGT
                     SENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIY
                     MSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNS
                     GLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLP
                     HSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEIS
                     GNNIDGVIVTVFPGSTHTNSRR"
     gene            1051..1536
                     /gene="vanZ"
                     /locus_tag="U4B05_002671"
     CDS             1051..1536
                     /gene="vanZ"
                     /locus_tag="U4B05_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN2051759.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1823..2152)
                     /locus_tag="U4B05_002672"
     CDS             complement(1823..2152)
                     /locus_tag="U4B05_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051760.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2457..2786
                     /locus_tag="U4B05_002673"
     CDS             2457..2786
                     /locus_tag="U4B05_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2051761.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2776..3063
                     /locus_tag="U4B05_002674"
     CDS             2776..3063
                     /locus_tag="U4B05_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2051762.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(3342..3905)
                     /locus_tag="U4B05_002675"
     CDS             complement(3342..3905)
                     /locus_tag="U4B05_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2051763.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1566 a          665 c          743 g         1474 t
ORIGIN      
        1 ttttttattg ttattgttat tcttaatata cttaggttat gactacgtta atgaagcact
       61 gttttctcag gaaaaagtcg aatttcaaaa ttatgatcaa aatcccaaag aacatttaga
      121 aaatagtggg acttctgaaa atacccaaga gaaaacaatt acagaagaac aggtttatca
      181 aggaaatctg ctattaatca atagtaaata tcctgttcgc caagaaagtg tgaagtcaga
      241 tatcgtgaat ttatctaaac atgacgaatt aataaatgga tacgggttgc ttgatagtaa
      301 tatttatatg tcaaaagaaa tagcacaaaa attttcagag atggtcaatg atgctgtaaa
      361 gggtggcgtt agtcatttta ttattaatag tggctatcga gactttgatg agcaaagtgt
      421 gctttaccaa gaaatggggg ctgagtatgc cttaccagca ggttatagtg agcataattc
      481 aggtttatca ctagatgtag gatcaagctt gacgaaaatg gaacgagccc ctgaaggaaa
      541 gtggatagaa gaaaatgctt ggaaatacgg gttcatttta cgttatccag aggacaaaac
      601 agagttaaca ggaattcaat atgaaccatg gcatattcgc tatgttggtt taccacatag
      661 tgcgattatg aaagaaaaga atttcgttct cgaggaatat atggattacc taaaagaaga
      721 aaaaaccatt tctgttagtg taaatgggga aaaatatgag atcttttatt atcctgttac
      781 taaaaatacc accattcatg tgccgactaa tcttcgttat gagatatcag gaaacaatat
      841 agacggtgta attgtgacag tgtttcccgg atcaacacat actaattcaa ggaggtaagg
      901 atggcggaat gaaaccaacg aaattaatga acagcattat tgtactagca cttttggggt
      961 aacgttagct ttttaattta aaacccacgt taactaggac attgctatac taatgataca
     1021 acttaaacaa aagaattaga ggaaattata ttgggaaaaa tattatctag aggattgcta
     1081 gctttatatt tagtgacact aatctggtta gtgttattca aattacaata caatatttta
     1141 tcagtattta attatcatca aagaagtctt aacttgactc catttactgc tactgggaat
     1201 ttcagagaga tgatagataa tgttataatc tttattccat ttggcttgct tttgaatgtc
     1261 aattttaaag aaatcggatt tttacctaag tttgcttttg tactggtttt aagtcttact
     1321 tttgaaataa ttcaatttat cttcgctatt ggagcgacag acataacaga tgtaattaca
     1381 aatactgttg gaggctttct tggactgaaa ttatatggtt taagcaataa gcatatgaat
     1441 caaaaaaaat tagacagagt tattattttt gtaggtatac ttttgctcgt attattgctc
     1501 gtttaccgta cccatttaag aataaattac gtgtaagatg tctaaatcaa gcaatctgat
     1561 ctttcataca cataaagata ttgaatgaat tggattagat ggaaaacggg atgtggggaa
     1621 actcgcccgt aggtgtgaag tgaggggaaa accggtgata aagtaaaaag cttacctaac
     1681 actatagtaa caaagaaagc ccaattatca attttagtgc tgaggaattg gtctctttaa
     1741 taaatttcct taacgttgta aatccgcatt ttcctgacgg tacccccaaa ctaagatttc
     1801 atagatagat tttctgataa ctttaaaaaa cacgaatagc ggaaaggatg tcttcattcg
     1861 ctaaagagta ataaactact tttccatctt gacgtgattt tgccattcca tgttttttta
     1921 aaaaacgtaa atggtgtgac gttgtagcga cactagcagt aagtatttct gctaaatcac
     1981 agacgcacat ttccttatag gtttctaaag catagaaaat tttaattcta gaaaaatcgc
     2041 tgaaacattt ccctaacaca agtaagtttg aaaaatcttt ttgatccaat ttatttttta
     2101 ttccatttac tttttcttta tgtacgacag tcacttggca aacttttttc attcatctca
     2161 cctcacattc aaacgattat ttgaatgaag tatacctatt tgatcactct tttgtcaatc
     2221 taccttaatt ccaatacact attataagca atttcatgta ttgaagtaaa cttacttgca
     2281 agtgcgtttt tgattccaag ttaaaaacat attattattc tacataattt cctaatcaaa
     2341 taaaactcct ttgaaaattg ttctaaagga gttttattat tttgtatttc tactaaaaat
     2401 agcgtacaat gtaattacta agtaattacg aaatgtaaaa taatggagga aataatatga
     2461 ttacaaaaac aagaaaacaa ggaaattcta ttatgttaac ggtccctaaa gaattcgatg
     2521 tgcccaatgg tgttgaagta gaagctaagc ttgtagagaa tggtatcctt tatgagtttg
     2581 tagagcctaa aaaagaattt tttgatttta gtgaagatat tttggctgat attctttcag
     2641 aaggttatag caaacaagag atcttaaaag aatttagaaa tagaaaaaat gcgttaactt
     2701 ctgcatttaa atctattgct gaagatactg ttgcaaattc taaaccgatg acaaaagagg
     2761 agttggcagc agaaattggc ttatgagatt ctaccttcta aacacgtaat aaagtatcta
     2821 aaaaaattaa aagagaaaac attaaaagaa caatttttaa cgataattta tgatgagata
     2881 gcagttcgtc ctcattcggg agaacaaaaa acgggtgatt tatcaggaat ttgggctatt
     2941 ggattcaagt atgcaggtac gacatatcgc gttgcatatg aaattaaaga taatgcagtg
     3001 atacctaact tactatgtgg aacgcatgaa aatttttatg aacaattaaa aaagattagg
     3061 taagaaccca atgtttcaac ttgagaggat agacgacact ttaaaagata atttttaaca
     3121 gctgagagtt gtaataccct ataattttaa accactaacc tatatattta tgaaaaatag
     3181 aaaaaattaa aacgcatttt aagcctcttt caaattaaaa gcttcatcct tatgcacaaa
     3241 gagggcttaa aatgcgttat tttatattgt tttttagtaa taataaaaaa atcactctaa
     3301 ttctcccata aatattatag attcctaaag attaaaatag attattccct attgtctttt
     3361 aagtagcggt agagtgttgc cttgctaata ccagtcattt ccgtaatttc ttttacgcta
     3421 taaacctgac ttttatacaa ttttactgct cgatcaacat ctttctggtt cactcgtgga
     3481 cggccacctt tacgacctct cgcacgtgca cttttcaaac cttctttggt acgttcaaca
     3541 attaaatcac gttcgaactg actaaatgct tggaagacag tcatcattaa gcgtccgtga
     3601 ggcgtgctgg tatcgaagtt ttctttcaag ctcaccagct taacatggtg ttcttcaaaa
     3661 taggtaacaa ggtcaatcaa atcctttgta ctacgaccca atcgagaaaa actttctaca
     3721 attaccgtat ctcccggacg aattttatct tttaaacgat ttagttgggg acgaaccgct
     3781 ttggttcccg tcattttctc agttaggatt tcattacaat tttgttcatt gagtaagtct
     3841 aattgacgat ttagttcttg tgaacgtgta ctcactcggg cataaccata aatgtatcca
     3901 ctcatgcttt tcttcctttc gtactttaat tctggcttga ttgtatcata aaagggagtt
     3961 atagatacat agttaatgca acaagttatg atacaaaata tgacctcttt tatttaataa
     4021 aagaggtcat ttcattttgt ctcataatcg attgtttttg atacgagtgt gtatacgaaa
     4081 taaacaaaaa aatcttaagt aaatttctag atacctaatt taatcctaac tagtgtatcc
     4141 taaagttaat aacgagttta tcacaatgaa aattataaaa tgctcccata ttagatataa
     4201 catgcaatat ttgaattaga actacaaaat ttattcgttt tataaagtaa ataatgttga
     4261 gatactaagg aatagaatat tataaggaat tgagctgaat gtattggata aaaaaagaat
     4321 aactttatta gagatggact ataaaaatgc tcgtaatttt cttctgaagt ctgaaagcta
     4381 tttgaatgct cagctaccag aatatattag ttttgacgaa actttaaaat ctgctaaaaa
     4441 agttttaa
//
LOCUS       DASJST010000183         4433 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJST010000183 DASJST010000000
VERSION     DASJST010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.185"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..2048)
                     /locus_tag="U4B05_002676"
     CDS             complement(543..2048)
                     /locus_tag="U4B05_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2051764.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U4B05_002677"
     CDS             complement(2045..2731)
                     /locus_tag="U4B05_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2051765.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U4B05_002678"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U4B05_002678"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN2051766.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DASJST010000184         4403 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJST010000184 DASJST010000000
VERSION     DASJST010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.186"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1015
                     /locus_tag="U4B05_002679"
     CDS             <1..1015
                     /locus_tag="U4B05_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN2051767.1"
                     /translation="DFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNLNVAMAD
                     RERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKTIEQIKI
                     DNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKN
                     YSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEI
                     GKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNY
                     ISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFN
                     NIS"
     gene            1160..1465
                     /locus_tag="U4B05_002680"
     CDS             1160..1465
                     /locus_tag="U4B05_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051768.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            2082..>4403
                     /gene="esp"
                     /locus_tag="U4B05_002681"
     CDS             2082..>4403
                     /gene="esp"
                     /locus_tag="U4B05_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN2051769.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
BASE COUNT         1614 a          602 c          819 g         1368 t
ORIGIN      
        1 tgattttaat gagatgtttc ttctatacat gtttaaaacc agatattact tatttggtcc
       61 atttagagca aataatattg ataaagattt ttttaaacta aaaatgaaca atttgaatgt
      121 tgcaatggct gatagagaac gactctataa ttcattgcaa aatttgactt tgtattcttt
      181 aggagacatt cgagatattc tgattttagt tcattatttt tttactggaa aaatagaaga
      241 tctattccat gaaccgttaa ttgaatatac aggaaatcta agtaaaacta tcgagcaaat
      301 caaaatagat aatctgttat cacaaaatta cgatccagaa atatatctgt ttctttatga
      361 aaataaaata ctggaatatg ttaaaaatgg agatattcga aatttagaaa acatggtttt
      421 taatctgagt aatggaataa ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa
      481 ttattctatc attgtttttg aaaagttagc acaaacgtct ataacattag gaatggatat
      541 aatagaagcg tatcaaagta gagatgcttt gatacaagaa aacgaattag ctgtatcgtt
      601 acctgaggtc ttaaaagtga gggattctgg gattgtttat tatactaagg aaatcggaaa
      661 aactaagata gagcatcttt ctcccttaat ttcttctgtc gttcaattca tcggcttaaa
      721 tatctataaa agaattactg taaaggaaat agctaattat ttttctgtaa gtgaaactaa
      781 attaagagaa agctttaaga atgaaatgca tattacgatt tataattata tctctaagcg
      841 aaaaatttct acggctaaaa taatgttgaa atcaaaccat actatcagtg aagtttcttt
      901 aggtttagga ttttcagatt cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt
      961 atctcctaag caatatcaat taggtttagt ggataatttc aacaacatta gctgagaggt
     1021 tttattttat tatgagacta tatcatttac gtagtttgga ggagttgagg aaattggatt
     1081 ttaacaaaaa attttttggg aaatttctga tttgagttgt ttcgtagaat ctgagcgtct
     1141 aagtaatggt aataatagtg tgttaaatga atatgacaaa gtagtaatta ataaagtaaa
     1201 aagtttatgt gaagaagcta gagaatctat cagtcatatt aatggtagca attttaagca
     1261 aacattattt aatctgatta agctagatgc taaaatatca agttatttat tctttttaat
     1321 acatgatgaa tttatggatt atcaaaaatt ggatcagtta attgaaagag aaagctggga
     1381 agattattac gtgggtctaa ctttttcaga aaagttaaac aactacaagt taatagatta
     1441 taagtatttg agtgaaagca attaattatt catgtagagt actagacact ttaaatatgg
     1501 acaattcaat tataaagaaa aagaatcaat aatttaatga ggctgtgaca aatgtcattt
     1561 tttgtttcgc taaaaacgag tgaaaaaatg ttgataactc caatcattcg tttaagattc
     1621 taggaatcat tcaggaatgc tgtgaaaccg atactttata attataaaga aggtgatttc
     1681 aaattccgaa gaaggaattt gaagtcacct ttttacttat gtcacagtct cattcagtaa
     1741 tgcgggataa ggctccttat tccgctattt gaatactctg aagtttaatc taagaaagag
     1801 taatattttg aaaacatgct tcatcgcata gaaatgaact taatttcaga aagaatacat
     1861 acatatcttt ttatatgttt tcagctgctg aatggttaaa aagcacaaat atccgaaaaa
     1921 aacatgaaga aatgtacttt ttttgttgta atgtgcatga aattctttaa gtaaactagt
     1981 taacataagt ttagtaagat tcctattctg tgaataatac gtatcagatt tatcttttat
     2041 tcttactaag aaaataacta ttaagggagt tgatttgaat aatggttagc aagaataata
     2101 agagagtatt tctagaaaaa acaaagaaac gagtattgaa gtacagtatc aaaaaattaa
     2161 gtgttggggt tgcatctgtt ttggttggtg ttggtcttgt cttgggaaca actgaactag
     2221 ttcaagcaca agatgaaatt tctccaagca ccccacttga aacagcgata agctccgtac
     2281 aagtaggtga caaagtagct tcggggaata cttttcagga gaatcctggt tatacgaaaa
     2341 attataactt ttcggattta caatttagtc ctcaagaact aactggagac acactaaaag
     2401 gcaatacaat tggatttgaa gtttacggaa aacataatat tgcagcatca actaaaaact
     2461 gggaaatccg tcttcaatta gatgaacgat tagctaaata tgtagaaaaa attcaggtcg
     2521 atccaaagaa aggaattgga tcttctagac ggacctttgt aagaattaat gattcgctgg
     2581 gtagacctac aaatatttgg aaggtcaatt atattcgtgc aagtgatgga ttatttgctg
     2641 gggctgaaac aactgataca caaactgctc ctaatggggt aattacgttt gaaaagagct
     2701 tagatgaaat ttttaaagag ataggcatag acaatctcaa gaccgatcgt ttaatgtatc
     2761 gaatctactt ggtaagtcat caagacgatg ataaaattgt accaggtatt gatagtacgg
     2821 gctacttttt aacagattca gatgatttct ataatagctt agatgtatct gaaaataatc
     2881 ctgaccagtt taaacatgga tcagtaagtg caaaatatga ggaacctaat actcaaacaa
     2941 aagatggttc aggatccaca ggtgctaatg gagctataat cttagatcat aagttaacga
     3001 aaaattataa tttctcatat tcagcttccg caaagggcac gccttggtat gctaattaca
     3061 aaattgatga acgattagtt ccatatgtag cgggtataca gatgcacatg gttcaggctg
     3121 acaaagtaac atatgatgtt tcttttgaat cagggaaaaa agtggctgat ttagcaattg
     3181 aacggcgcaa agatcatgaa aattatggtg taggttcaat tactgataat gatcttacca
     3241 aacttattga ttttgctaat gcaagtccac gtccagtcgt tattcgatat gttttacaat
     3301 tgactaagcc attagatgaa atcttagaag atatgaaagc aacagcacaa gttgaagaaa
     3361 ataaaccatt tggtgaagat ttcatctttg attcttggtt gtcggatacg aataaaaaat
     3421 taatccagaa cacttatgga acaggttatt attatttgca agatattgat ggtgatggaa
     3481 accctgacga taaagaagag agcggagaca cgaatccata tatcgggaaa cctgaattag
     3541 aagaagtata tgatgttgac acaacagtta aggggaaagt attcatccac gagttagcgg
     3601 gaacaggtca caaagcccaa cttgttgata aagaaggtac tgtattagca gaaaaaacta
     3661 tcgctccaaa tgaaaaagat ggggctccaa tttcagatac tgtagaattt gaatttacgg
     3721 gtgtagattc aagtaaatta atcgcgaaag atgaattaaa aatccaaatc gtttctccag
     3781 gttttgataa accagaagaa ggttcaaccg ttattaagga atcaccaaaa gcggttgata
     3841 aacaaaccgt ggtagttgga tttaaaccag atgctaaaga atcaattcgg aataataaaa
     3901 acttacctga agatgcagag tattcatgga aaacagagcc tgatacttct aacgttactg
     3961 atagtacgaa aggtattgta actgttaaga tcggaaatcg aactttcgac gtggatgtag
     4021 agtttgctgt aaaagcttct caagctatgg aaaatgatgc aacatacgta cctataacaa
     4081 caactccaga aacgacagtt caaagtggaa aacctacatt tgataaacca gatgttcctt
     4141 tagctaaaga tgccttttca attttagatg tctataataa ggacttcggt aatgcaagtg
     4201 ttgatgcaaa tactggtatt gttacattca ctccagctaa aggtgtagga gaatcggagc
     4261 cgattactgg aacaattcct attaaaattg tttaccaaga tggttctgta ggcacgaccg
     4321 atttagcagt aactgtaagt aaagatattt atgaaaatcc aggagaaaac attcctgcag
     4381 gctaccacaa agtgaccttc acc
//
LOCUS       DASJST010000185         4359 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJST010000185 DASJST010000000
VERSION     DASJST010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.187"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            153..590
                     /locus_tag="U4B05_002682"
     CDS             153..590
                     /locus_tag="U4B05_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051770.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            685..1569
                     /locus_tag="U4B05_002683"
     CDS             685..1569
                     /locus_tag="U4B05_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2051771.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1791..>4359
                     /locus_tag="U4B05_002684"
     CDS             1791..>4359
                     /locus_tag="U4B05_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051772.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
BASE COUNT         1429 a          728 c          969 g         1233 t
ORIGIN      
        1 agaactaaat caacagtagt ttaattatta cttgatttag ttctttttat tttaaaaatt
       61 ttttttactt agaatccagc caccctagat atttttctgt ataatcttaa ataaagaaaa
      121 cgatattaaa aaatgcacgt agaagtagat aaatggagtg gttttgcaaa tttcttttat
      181 ttctagaagt tattcacgta tacggatttg tttttctcac aaataaaaag gcaggaagta
      241 caatggatca tttgtatata aaatatattc atcaattttc cgttttggta ttaggcacac
      301 taattatagc ggggattgtt gcatttatta ttcaaggtcg tgacatatta acagtacatc
      361 aagagttttt cagttatgca tttggtattc ctctgatcgc tataaggtct gataacaaaa
      421 agtggaagaa acgctattac attctcttgc tatttttgtg tttttatctg ccacctaaga
      481 tttttacttt attaggtata caatgcctat cattttcttt tatttcctat atcattgttt
      541 taattttcct cagtttcata gtatgggtat tttatcttgt caaaaaataa atgaatgttt
      601 catgaacagt acttcatcac agcctaattt ttttctttat gctatcgtta tattaaagaa
      661 atcaatcaga agggaaagaa agtgatgaaa aaagttttat tagtggtaaa ccccagttca
      721 ggcggtgaac aagcaaagga atttgagcaa ttagcgatag caaaattgga atcggtgttt
      781 gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt tactcgcgaa
      841 gcggctacgg aagggtatca tagtgtgttt gtcatgggcg gagatggaac agtcaacgaa
      901 ggaatcagtg gaatcgctga acaagaacat cggccaaatt tcggattttt cccattgggt
      961 acggtgaatg acttggctcg agcattaggg ataccgctag aacctgagga agcgatcaat
     1021 catttttcga ttgaatcagt caagccattg gatattggaa aaataaatga cgactatttt
     1081 atgaatgtcg tcgcaattgg ctctatccct gaagcaatca atgatgtaga tgcggagaag
     1141 aagacgaaat ttggaaaact agcttatttc atgtcaggaa tcaaacagtt agcttccaca
     1201 caatcctact cgtttcacgt agaagttgat ggaaagaagg aagaaattga gagcagtatc
     1261 ttgctgatag gactaactaa ctcagtaggc ggttttgaaa cattacttcc aaatgcaaaa
     1321 gtggatgatg gtaagctcca tttggtttat ttaaaagatt cttctttgat cgatacgctt
     1381 aaaacattgc cagacctatt aaaaggagta gatgaatcta cgaaaaatct agcctatctt
     1441 acttgtgagc aattagttgt gtcattagct gatgaggaag aattgacgac gaatgttgat
     1501 ggagatgaag gggataaatt acctgtgaca atcaaagtgt taccctcaca tttacaggtt
     1561 tattgttaaa atggaggttg tgaccgaaga gttgtcagga ggacatcggt tgttcggaat
     1621 aagggactgt gacaagactt ttgtcacagc ctttttttgt acaaaaaaga aattttcacc
     1681 taacagaaaa atttgccata aaaatacata ttttaaatat gaaagtacaa ttacaaataa
     1741 attaaaacgt taaaataaaa ttgtcaaaac gtcagagagg atggtggaga atgaatcaat
     1801 atgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc tctcttgatg gtgacatccg
     1861 tttttgccga tgtagagaaa acaagcgaat caacgcaatc agagaatcaa agttttgaca
     1921 agactacttt ttcagaagaa tcaattactt ctgaaactac agaatcccaa agtgaggcgc
     1981 ctacttctaa tacaaggcaa gaagaaaata agacaagtga gacctcagaa gtagctgaac
     2041 aaacgtctga tagcagtgaa gaggtacagg ttagagttgt ttcagcggat gggtacagcg
     2101 aagctgccac aacagaagaa ctgacgcagc ttctcgcaga cgaatcagta gctaagattc
     2161 gtttaactca gcctttgact ttagacaggg aattggagat caagcgagat atcgttatcg
     2221 attttggcgg acttgctcat aatttcggca cacatcacat ctatatcaat gagacacctc
     2281 agtcgatcga gtttcagaat tttaaaggaa cggcagctca tccaggtggg ttgataccaa
     2341 atatcgatgg aaatgccatt atcattgctt atatgtccga tttttgggga aacagatatc
     2401 attttacggg agaaataaaa tttaccggga cacttgactt atatgatggt tctaaattag
     2461 gcctgattta cgcccctcgt gcaactgtca cattagatgg tgtttccggt gtattggatg
     2521 tccaaccagt gaaagaaggg atgaatgccg ctccgggtaa agcttatttt tgtagaagct
     2581 atcagctaaa tgtaatcaat ggtagtcagc tttatggacc atatctagga aaattctatg
     2641 gttttctaga ttcaggtgat gagacgggca gtggaaaaac agcaccagga ctccatatct
     2701 tgagtggttc aaaggtctct cttgactatg atcgggcaga tggcgtcagc gatgaaggag
     2761 aggctgtcga tacgctaccc gggaatgtca catttaaagt cagtggctca ggaagtgaat
     2821 tttcggtaaa tacaaagatc aatattacca acgacaacaa tcgcggaatt atccaaatga
     2881 gaggctcagg atcaagagtc gctgtgtcga atgatgggaa aatcacaatc aatacagcga
     2941 ctactggcgg atttcgcctg caaggagatt ctagtcaaat ccatgtatca agtggtggac
     3001 aaatctcggt caaaatggct ggagatggcg accaacctgg aaacaatggg atgcgttttg
     3061 tagggaaagg actttctttg atcgtagaag gagccggatc agtcatcgat attgataagc
     3121 aaagtggtcg ttctagcgca gttcgttttg agaacggtac gcaaaaacta acagtaacta
     3181 atggaggctc attgaaggta agaaatgcag gggatggcaa atcttatgtg aacagactca
     3241 atcaaggaaa ccaagcgatc caattttatg acgcttctgg ttttctagaa agtccagggt
     3301 cagctgattt tacggttact ggagaaaaat caaatatcga tatacgcgca gattccggag
     3361 ctaccgtaga tacaactgga gatattgatt tgaactttat ggctggcgaa aaaacttatt
     3421 tagtcatggt cgggaaaact ggggaagata agactggcat catcagtggg gatgtgttga
     3481 atgtagactt agaatctcct acctatttcg atttccaaaa caaacggatc ggaagcgaga
     3541 ctaatggcat aggtggctgg gtatttcaag gaaatggcga ttcttctttg aagatcgctc
     3601 attcagaaat tgctttatgg cgaaaggcag gagtaacgtt cgataacttt gaaaaagatc
     3661 cggattattt ttctgatatg acagacttat cgatcaatgg aaccgactta ggtaattttg
     3721 tatcaagccc tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc ggaatgaaaa
     3781 attataatcg aatcagtgcc aataaccagc ttccgatgat tgaatcctta agagtaccaa
     3841 cgaatgcaga taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc gaggcaaagc
     3901 caagagatgc ttggacaggc gaagtagaag tgactttacg tattacttat gcagatacga
     3961 cgaaatcacc tgtagaaatt actgctctca cacaaggaca aacagatgaa aatgaaggga
     4021 caggatataa tccgtgggga gaaggcgaac aaggcggttt gtttgaggtt caagtaccca
     4081 acgaagaaag actaacaacg ggggatcaag tgcaaatagt tgctgctaaa aaagtaagag
     4141 gcggccaaga agtgacagaa ctggactctg ccaaaacgac agtcgatgtg attcctcctg
     4201 agcctgctaa attaagtacc acattgatca atgcagcttc tcgctcaatc acaggtacag
     4261 gcgaagcagg agctttggtt tcattgaaaa aaaatcaagc tgttttgtcc gaaacaacta
     4321 ttgatgatac aggtaaattt gttctttcta tcccagaaa
//
LOCUS       DASJST010000186         4332 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJST010000186 DASJST010000000
VERSION     DASJST010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.188"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..696
                     /locus_tag="U4B05_002685"
     CDS             85..696
                     /locus_tag="U4B05_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEN2051773.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(798..1706)
                     /locus_tag="U4B05_002686"
     CDS             complement(798..1706)
                     /locus_tag="U4B05_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2051774.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(1748..2062)
                     /locus_tag="U4B05_002687"
     CDS             complement(1748..2062)
                     /locus_tag="U4B05_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2051775.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            2361..4292
                     /locus_tag="U4B05_002688"
                     /pseudo
     CDS             2361..4292
                     /locus_tag="U4B05_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
BASE COUNT         1396 a          714 c          844 g         1378 t
ORIGIN      
        1 atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat ctatctatga
       61 aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat cgctgtttat
      121 atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca aagccacaca
      181 aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat tttggtagcc
      241 gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat cattggtctt
      301 ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga agaggaggaa
      361 gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt attctggaca
      421 gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat tccatacttt
      481 acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat ctctgttgcg
      541 gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga aacgttagaa
      601 aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt tattatgata
      661 gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg aaagataaaa
      721 agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa tgaagtattt
      781 tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc ttctttgctt
      841 gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac ttctgcaatt
      901 tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac cgtgatgata
      961 agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc ttttacagca
     1021 ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg cgaaccattc
     1081 aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat ataaagtgca
     1141 atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat tagaatactc
     1201 acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt caacatgctc
     1261 tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa tgcaatagct
     1321 acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat caaacgtggc
     1381 acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt tatcagcgcg
     1441 cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt tttctctgaa
     1501 taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc atgaactgcg
     1561 tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct tatagagaaa
     1621 ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg attcgagtga
     1681 ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg ctacatacta
     1741 attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga atactaaaaa
     1801 catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat ttcactaagc
     1861 gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg tccagagaaa
     1921 tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc gacaatcgtg
     1981 taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct ttatcgattg
     2041 gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt ccgttcatat
     2101 ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag aagtggcgtt
     2161 ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac atatgattaa
     2221 ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga aaggagtcag
     2281 aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg aattataaat
     2341 tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa gaaaagcata
     2401 atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc gttttgtact
     2461 ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat cagttggtac
     2521 aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt cttcttgaag
     2581 gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct aattctcata
     2641 ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg gaaggaacat
     2701 tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa ggaagaagca
     2761 aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta atcctgcctg
     2821 atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa cttcaagtgt
     2881 tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca tccattgatg
     2941 agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat ggcgtcttcg
     3001 ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa aacaaagata
     3061 cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa acaaaagctg
     3121 cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt gccattcctc
     3181 tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt gtctatagag
     3241 gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact gtctcggcaa
     3301 cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt agttcttacc
     3361 tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc ttgaccaaag
     3421 gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa aacatgattg
     3481 atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct attctaagaa
     3541 agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt aaaggattga
     3601 cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt gatgatgttt
     3661 cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca gtcgtatacg
     3721 tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg agtgaatatg
     3781 ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta attactggtg
     3841 actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa gtgattgcga
     3901 atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa tacggcgtga
     3961 ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca gatgtgggaa
     4021 tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt ttaatgcaaa
     4081 acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac cgtgttattt
     4141 gacagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt agtttcttag
     4201 gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg gttgttattt
     4261 tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt caaattaatc
     4321 gagtaataga ga
//
LOCUS       DASJST010000187         4274 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJST010000187 DASJST010000000
VERSION     DASJST010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.189"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            635..1021
                     /locus_tag="U4B05_002689"
     CDS             635..1021
                     /locus_tag="U4B05_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2051776.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1119..1310
                     /locus_tag="U4B05_002690"
     CDS             1119..1310
                     /locus_tag="U4B05_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051777.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1824..2156
                     /locus_tag="U4B05_002691"
     CDS             1824..2156
                     /locus_tag="U4B05_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2051778.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2453..2608
                     /locus_tag="U4B05_002692"
     CDS             2453..2608
                     /locus_tag="U4B05_002692"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN2051779.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2884..4224
                     /locus_tag="U4B05_002693"
     CDS             2884..4224
                     /locus_tag="U4B05_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2051780.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         1486 a          789 c          728 g         1271 t
ORIGIN      
        1 actattcctc tgtcaaaata agctaactta cttcattata aaatatccac ttatcgcttt
       61 catgtatatt ccaccgtaaa aagtacattt tatcaccgag aactcatcat ttttcatcat
      121 gatgaatact atttacatat atctagttca aagacaataa ttattatgat ccgttattat
      181 ctaagcacta tacagataat aggaattttt cgtttctaaa tactccaatt tgggaatcca
      241 ataaaaattt tcgttcaaat acgtgtctat tcgaactctt ttctagttgt taattatgaa
      301 gggggaagaa ataaaaaata caatctatac acgaataaga atattatcca aaaggaggtt
      361 ttagaaaaga tttattatac gtaaaaagct caagacattt tcttagccat aaggacatgt
      421 ttgatattta agaagatata tgaattttct tttctaagta taaagataac tatccattat
      481 caactaaaaa aacatttttg aaaataaaca tcagcagtcc tatcaatgca tgtgagctta
      541 tagaagttat tcgcagcgca gcataattgc attagcttct tttcgtgttt tttgcctctt
      601 ttccctttaa atcttaatgg aggaggtgtg caatatgtca gataacgacg aacgcgtaaa
      661 tgcccagttg attcgattct ttgaaaaggt cattttgaac accgctaata cttattataa
      721 aaaacaagaa gaaattcaaa aacatgaaca acttgatcaa ctcccaccat atttgtttac
      781 taaagatact ataaaattta aacaaccgat taatgtttta aatgtgactc tccttgttga
      841 agatataaaa ttagcaaaaa tccttccttt gttgaaagaa cctgaacaaa tttttcttat
      901 cgaaaaattt atctttaata aaactgataa agaaattgga aaggttctgg gtattacaag
      961 acagggtgcg acaaatttaa aacatcgact ttacaaaaaa attcaaaaaa tgctggttta
     1021 atatctgcat aataactaat tttgtatgct tctttgctcg tcctcctcct tccccaaaaa
     1081 ataaatagat tataagttca tgggaagaag gtaaatttat gaatacttta aaagaagatt
     1141 tcattttagc aaagaatgga aatgaagaag cagttgtggc cattctcaaa agatttagcg
     1201 ctttggtcca taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat
     1261 gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag
     1321 ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaagccat
     1381 cttctttata attataaagt atcgatttta cagcgttcta taatgattca tagaatctaa
     1441 aacgaatgat tttaattatt aacgtttttt aattcgtttt tagcttaaaa ataacttttg
     1501 tcacagtctc actcctaaaa ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc
     1561 agaactgggc catggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
     1621 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
     1681 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
     1741 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
     1801 gttaccggaa ggaatgaact ctattgaaaa aaacatatga tcgtaaattt aaactaaaga
     1861 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
     1921 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
     1981 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
     2041 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
     2101 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
     2161 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
     2221 tagagaaaaa acaatcaaag aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa
     2281 ccaaattgat gctgtttggg tgacggatat tacttacatt ccatgtaatg acggtcgcct
     2341 ctatttgtcc acctatattg acttagcaac tcgtattcta cgttactata tggtggattc
     2401 acacatgaaa aaagagattg tcgtccgtcc attacaaatt tattaaggag aattgcctac
     2461 ggtaattcat tccgatagag gtagccagca tcggtctttt atttatcaag agctgttgac
     2521 cgaccaccac atcacacatt caatgagcca aactgggact cctgttgaca atgcagtaat
     2581 tgaagcgttt catcgatcaa tcaaatgaga attaatcgac tccaacaaat ataaaagtaa
     2641 aatagagatg aaggtgttaa tccaagatta tttagaagac tactacccaa acaaaagaat
     2701 ccatacaaaa tttatgatga ctccttgaca atttgaagcg aagaaaataa gggatctata
     2761 atatctagtg ttggtgacgc cttctgttgc caatactttt tcttttttct aataaacgca
     2821 aacagacacc ttgaaatcct ttagaataaa gtcgacgaaa accattcaaa ggagtgtttc
     2881 aagatgtcct attcagagtt taccaaagag ttactagata ttctagacct aaatcttacc
     2941 tttcatgaag atgcctttcg caaagagcgc attaatgacg aaacatgttt tgtttttgat
     3001 ggcacattga cctatcaacc agaggaatgc ttccattgcc actatcaaaa taagcaaacg
     3061 atcattaaat ggggctggaa aaaagtatcc atcttattga atgatgtcag taactacaag
     3121 accattgtgc ggattaacaa acagcgcttc aaatgtaaac actgtggaaa gactttttta
     3181 gcagaagatt cagttagcga tcgtcgctgc tccattgccc gcagagtcaa acaagccatt
     3241 cttgagctat taagtgagcc tatttctatg tctttgattg cacgtatgaa gcacatttct
     3301 ccgaccactg tcattcgaat acttcgcagc cttcggccta aaaccgtttc tttgaaccag
     3361 ccgttaccag aagtggtgtg tttcgatgaa ttcaaatcgg tgaaaaacgt ctcgggagcc
     3421 atgagctttg tcatgatgga cggcaagacc catcaactga ttgatattgt ggaaaatcgg
     3481 caactcaatc ctttacgcga ctatttcttg cgctacccta gaaaagtccg tgaacaagta
     3541 cgtctagtgg tttcagattt ctatacaccc tatcgcacat tggtgaaaga gtgttttccg
     3601 aatgcccgaa tcgtcgccga tcgctttcat attagccaac acatcggtcg ggcctttacc
     3661 aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg atcgtcggtc gaagcactta
     3721 aagaaatact ggagactact tcaaaagaat gcgtgggaat tgaatggtca gcatcgctac
     3781 tggcgtccgt catttcgaga tcatctgaca gaagcggaga tagtcgatcg gctattatat
     3841 tatgatgact ccttaaagcg aggctacgaa gtctaccagg tttttctttc cgctatcaga
     3901 agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag actacaagga gttgccagag
     3961 cactatcaac cagtgttcac gaccttcaag aaatatcgaa cagaaatcaa acgagcgttg
     4021 cgagttccct attccaacgg ccctatagaa tgtttgaaca accatatcaa agtactaaaa
     4081 cgaattgcct atggctttag aaactttcaa aactaccgag aacgaatctt tttatatcga
     4141 ggaaaatatt tcaagaaaac taagaacact acccaactga ctaaagccag aacaacaaca
     4201 cggctagaca agatagctgt ctagccgtgt gagtatctcg attattctat tgaatttctt
     4261 caccaacact catt
//
LOCUS       DASJST010000188         4230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJST010000188 DASJST010000000
VERSION     DASJST010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.191"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1022)
                     /locus_tag="U4B05_002694"
     CDS             complement(45..1022)
                     /locus_tag="U4B05_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN2051781.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="U4B05_002695"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="U4B05_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN2051782.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            complement(2595..4019)
                     /locus_tag="U4B05_002696"
     CDS             complement(2595..4019)
                     /locus_tag="U4B05_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2051783.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         1168 a          894 c          736 g         1432 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc gaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataagggact gtgacaaaaa tcttatcaca gtcccttatt
     4141 ctgaataatg atgttcaaaa gcttgtatct gcgataataa ttccagccaa acaaaacata
     4201 taaagaacta tttttgtttg gctgttctta
//
LOCUS       DASJST010000189         4223 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJST010000189 DASJST010000000
VERSION     DASJST010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.192"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(383..1669)
                     /locus_tag="U4B05_002697"
     CDS             complement(383..1669)
                     /locus_tag="U4B05_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2051784.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1662..2930)
                     /locus_tag="U4B05_002698"
     CDS             complement(1662..2930)
                     /locus_tag="U4B05_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2051785.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            3182..3643
                     /gene="mscL"
                     /locus_tag="U4B05_002699"
     CDS             3182..3643
                     /gene="mscL"
                     /locus_tag="U4B05_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN2051786.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3702..4148)
                     /locus_tag="U4B05_002700"
     CDS             complement(3702..4148)
                     /locus_tag="U4B05_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2051787.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1195 a          863 c          740 g         1425 t
ORIGIN      
        1 ccaaccaagt taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc
       61 agggcagctt tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat
      121 aagccgaaat atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
      181 agctgaccac ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag
      241 ttgattaaaa gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag
      301 aataccataa aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag
      361 acttttcctc ataaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
      421 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
      481 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
      541 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
      601 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
      661 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
      721 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
      781 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
      841 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
      901 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
      961 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
     1021 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
     1081 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
     1141 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
     1201 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
     1261 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
     1321 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
     1381 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
     1441 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
     1501 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
     1561 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
     1621 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
     1681 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
     1741 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
     1801 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
     1861 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
     1921 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
     1981 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
     2041 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
     2101 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
     2161 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
     2221 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
     2281 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
     2341 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
     2401 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
     2461 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
     2521 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
     2581 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
     2641 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
     2701 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
     2761 tgatccggat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
     2821 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
     2881 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
     2941 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
     3001 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
     3061 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
     3121 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
     3181 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
     3241 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
     3301 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
     3361 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
     3421 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
     3481 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
     3541 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
     3601 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
     3661 agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc
     3721 tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat
     3781 ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct
     3841 ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct
     3901 ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat
     3961 actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttgt
     4021 ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt
     4081 cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga
     4141 tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa
     4201 aaagcaaacg aaccttacga atc
//
LOCUS       DASJST010000190         4092 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.193, whole genome shotgun sequence.
ACCESSION   DASJST010000190 DASJST010000000
VERSION     DASJST010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.193"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..533)
                     /locus_tag="U4B05_002701"
     CDS             complement(54..533)
                     /locus_tag="U4B05_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN2051788.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            892..1674
                     /gene="rpsB"
                     /locus_tag="U4B05_002702"
     CDS             892..1674
                     /gene="rpsB"
                     /locus_tag="U4B05_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN2051789.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1773..2654
                     /locus_tag="U4B05_002703"
     CDS             1773..2654
                     /locus_tag="U4B05_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN2051790.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2790..3512
                     /locus_tag="U4B05_002704"
     CDS             2790..3512
                     /locus_tag="U4B05_002704"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN2051791.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3515..4072
                     /gene="frr"
                     /locus_tag="U4B05_002705"
     CDS             3515..4072
                     /gene="frr"
                     /locus_tag="U4B05_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN2051792.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1466 a          724 c          885 g         1017 t
ORIGIN      
        1 aaaatattga aaataaaaaa acacgcattc catattagga atacgtgctc tgattaaaaa
       61 agtgatactt cagttggatg gtgaagaaat tcttctactc gttttgcgtc ctcctcattt
      121 tccgagatga ccatgatcgt atcgaatcca gcaattgtac tgacaataga gggaggtttg
      181 atctcatcta aaacagatgc aaataaatga gcattgtctg gcaaggtatt gacgatcgtc
      241 aaaaagtgga ctcgttcgac tttcaataca atgtcttcaa tcatttgaac cagccgatcc
      301 tcttcctcag tcctagtctc aggagttcct ttttcgcgaa acaatacgaa gcgggaaacg
      361 ccattttcat caggtgcctt cacgattttt agatcacgga tatctcgaga aatcgttgcc
      421 tgcgtggcgg taacgccata atgcccaagt ttgtttaata gttcttcttg cgtacggatc
      481 gggaattctt caatgacttg tttaatcagc aaatgtcttt gagctttgcg cattttcttt
      541 ctcctttacg aaagatttca catgtatcat aacaaaaaaa agaaggattg cccaataata
      601 ttcgtctttt atacctaaaa ttattttatg gaaaaaggat aataaagaaa caaagagaaa
      661 tctactgttt ttcttgaatt ctttttatct ttcaggtaaa ctattgaatg ttgagcagaa
      721 aagtatattt gctcttgcga tacggatctt ttgtctgtat aatagctgag ggtgctttat
      781 gcatcagcaa atccacattc ttattggatt gtcggtaggt gcttgaaaaa gtttgccggc
      841 aaggtagaag ataagaagag gaaaaataaa accaaatcgg aggaaacaaa catggcagta
      901 atttctatga aacaattact agaagccggc gtacactttg gtcaccaaac tcgtcgctgg
      961 aatccaaaaa tgaagaaata tatcttcaca gaaagaaacg gaatctacat cattgacttg
     1021 caaaaaacag ttaaattagt agatgcagct tatgattaca tgaagagcgt tgctgaagaa
     1081 ggcggcgtag cattattcgt aggtacaaaa aaacaagcac aagaagcaat caaagaagaa
     1141 gcaactcgtg caggacaata cttcgttaac catcgttggt taggcggaac attgacaaac
     1201 tgggatacta tccaaaaacg tatcagccgt ttgaaacaaa tcaatgcaat ggaagaagat
     1261 ggaacttttg aagttctacc taaaaaagaa gttgctggtt tgaacaaaga acgtgaacgt
     1321 cttgaaaaat tcttaggcgg tatcgctgat atgccaagaa tcccagatgt aatgtacatc
     1381 gttgacccac gtaaagaacg tattgctgtt caagaagcac acaaattaaa tatcccaatc
     1441 gtagctatgg ttgatacaaa ctgcgatcca gatgaaatcg acgtagtaat cccttcaaac
     1501 gacgatgcaa ttcgtgccgt taaattgatt acttcaaaaa tggctgatgc tttcatcgaa
     1561 ggaaaccaag gtgaagatca agtagttgaa gaagatttca cagaagaaaa cacagcaaca
     1621 tcaatcgaag aaatcgtaga tgttgttgaa ggcgacaatt cttcagcaga ataattttga
     1681 atgatggagc tgtttcaaag gctgggcgac aagcctcact ctcctttgaa gcagcttttt
     1741 ttaaaaagaa aaacacacag gaggagttaa acatggcaga cgttacagct aaaatggtaa
     1801 aagaactacg cgaaatgact ggcgtaggaa tgatggatgc gaaaaaagca ttagttgaag
     1861 tagaaggcga catggaaaaa gcagttgatc ttttacgtga aaaaggaatg gcaaaggctg
     1921 cgaagaaaaa tgaccgcatt gcagctgaag gattggcttc agttgctgtg aaaggtaaca
     1981 ctgcagcaat cgttgaagta aactcagaaa cagactttgt ttctaaaaac gaaatgttcc
     2041 aagatttagt aaaagaaatt gcagaacttg ttgctgaaaa caaaccagca gacatggaag
     2101 ctgcaatgaa aatcaaaaca gataaaggta caattgaatc tgacttgatc gaagcaactc
     2161 aagttatcgg agaaaaaatc agcttccgtc gctttgaagt tgtagaaaaa gacgacaatg
     2221 ctgcatttgg tggatacttg catatgggcg gacgtattgc cgtattgact gttctagatg
     2281 gaacaactga cgaaacagtt gcaagagacg tagctatgca cgtagcagct atcaatcctc
     2341 gttatgtaaa cgaatcacaa attccagaag ctgaattaga acatgaaaaa actgttctta
     2401 cagaacaagc attgaacgaa ggcaaaccag cgaatatcgt tgaaaaaatg gtagaaggac
     2461 gcttgaagaa attcaaagca gaaatcgctt tggtggacca accattcgtt aaagatcctg
     2521 atatgactgt tgaaaaatac gttgcatcaa aaggcgcaac tgttaaaaca ttcgtacgtt
     2581 tcgaagttgg cgaaggtatc gaaaaacgcg aagataactt tgtagaagaa gttatgagcc
     2641 aagtgaaaaa ataattctgt tgaataacag acgggaacgc acggtttgtg cgctcccttt
     2701 ttttaagcaa aacaagaaaa accagtctta gagtgtcgaa atcatctaag tatggtaaaa
     2761 tggcaataga aaatattgga ggaatgaaca tggtcacacc gaaatatcag cgtgtagtct
     2821 taaaattgag tggagaagct ttagctgggg acgcaggatt tggaatcaaa ccaccagtaa
     2881 tcaaagaaat catccaagaa atcaaagaag ttcacgagct gggcgttgaa atggcaatcg
     2941 ttgtgggcgg cggaaatatc tggcgcggac aaatcggagc acaaatgggc atggaacgtg
     3001 ctcaagctga ttacatgggt atgttagcta cagttatgaa tgccttagct ttacaggata
     3061 cgttggaaaa cgtgggtgta ccgacacgcg tgcaaacttc gattgaaatg cgtcaaatcg
     3121 cagagccata tattcgccgt aaagcagaac gtcatttaga aaaaggacgg atcgttatct
     3181 ttgcaggcgg taccggcaac ccgtacttct cgacagacac aacagcagca ttacgcgcgg
     3241 cagaaatcgg cgcagatgtt atcctgatgg ctaaaaacaa tgtagacggt gtttattctg
     3301 ctgatccgaa agtagatgca aatgcagtaa aatttgaaga actgactcat ctggaagtta
     3361 ttgccaaagg attacaagtc atggattcaa ctgcaagttc actaagcatg gataatgata
     3421 ttccactgct tgtcttcaat ttaaatgaac acggaaatat tcgccgtgca atcttaggcg
     3481 aaaatatcgg aacaactgta agggggaaat aaaaatggca gatgcaatta tgacagaagc
     3541 aaaagaaaaa atgaaaaaag cagcacaaaa tcttcaacgt gaacttgggc aaatccgcgc
     3601 aggccgtgcg aacgctagcc ttttggaccg tattactgtg aactactacg gtgcgccaac
     3661 accattgaac caaatggcat caatccaaat tccagaagca cgtgttttaa tgatcacacc
     3721 ttttgacaag agcattcttc aagatgttga aaaagctatt atggcaagtg atatcggaat
     3781 cagtccaacc aatgatggaa atgtcattcg cttagttatc ccgcaactga cagaagaacg
     3841 cagaaaagaa ttagctaaag atgttaaaaa agaagcagaa aatgcaaaaa tcgcgattcg
     3901 taacatccgt cgtgatgcga tcgatgaata taaaaaacaa caaaaaaatg gtgatattac
     3961 cgaagatgat ttgcgcggat tagaaaaaga cgtacaaaca ttgacagata acagcatcaa
     4021 agaagtagat aagatcgctg ctgataaaga aaaagaatta ttggaagttt aataaaatca
     4081 taaaaacaga gg
//
LOCUS       DASJST010000191         4067 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJST010000191 DASJST010000000
VERSION     DASJST010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.194"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            86..1435
                     /locus_tag="U4B05_002706"
     CDS             86..1435
                     /locus_tag="U4B05_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2051793.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="U4B05_002707"
     CDS             complement(1563..2153)
                     /locus_tag="U4B05_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2051794.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="U4B05_002708"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="U4B05_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2051795.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1173 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaa
//
LOCUS       DASJST010000192         4042 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJST010000192 DASJST010000000
VERSION     DASJST010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.195"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..432
                     /locus_tag="U4B05_002709"
     CDS             <1..432
                     /locus_tag="U4B05_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051796.1"
                     /translation="IFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKN
                     IYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNH
                     RQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            446..1135
                     /locus_tag="U4B05_002710"
     CDS             446..1135
                     /locus_tag="U4B05_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051797.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1260..3611
                     /locus_tag="U4B05_002711"
     CDS             1260..3611
                     /locus_tag="U4B05_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN2051798.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3691..>4042
                     /locus_tag="U4B05_002712"
     CDS             3691..>4042
                     /locus_tag="U4B05_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051799.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRF"
BASE COUNT         1402 a          518 c          827 g         1295 t
ORIGIN      
        1 attttttcag tatttacaat attaattggg ggctattttc ttttgaattt agattctgtt
       61 aattcaaacg ctcaaataaa agatcaaaga gtagctgaac ataataataa aaaaatgagt
      121 ttgaccaaaa atatctattc tatgtcagat gaagaattgt ataaagaatt tggtgattta
      181 tccggggaac agttagaaga aagtattcca ggaattagtg actcagataa aattgttgtc
      241 ttgtctgctg gtgggcaatt aatgggagat ggcaaatccg aattagttag ttataaacaa
      301 aatcacagac agtttattga tgttaatact gattccaacg ctatttcgat taaggatcta
      361 aaagagcttt taaatagtaa aagaaatgaa ttaaagcaga taaaagaaac taattcagtg
      421 gaattagagt aaaggaagat tcattatgaa aaagattttt ggtgtttctg ttgggctact
      481 tatgatgtta acatttggaa atgcagtata tgctgctgag aaagaagtta tttcagatcc
      541 agagcagtac gcctcacgat actttgctga ttttgaagaa gaagataaag tacttgcatt
      601 gccagaaggt ggttttttaa aaggggaagc taaaattgta tctatggata atccagatat
      661 aattcaaggt tcatataatt cagaaactga tcctgcttca gtttcagtga cagaagcaaa
      721 aaaaattgtt gctgaaaaat tcaaaaatga tattgaaact actccaatta tgcctcgagg
      781 agcatttcca cccgatgttg gtactagcgg acaccttctt gtactagcag gaggaggaag
      841 ctataaatca cagccattta gtggtgcggg atggcgtttt ggaggtatgg gtgtaaaacc
      901 agctgatggt acaggcaact ttttacgttg gaaatcctac attgacgatg gaagagtagg
      961 aaatgtaaat caagccaacg caactaaatc tggttctatt caaggaatgg cattgtatgc
     1021 tcgtgaagga ttcaaatact ttgcaccaaa cgggcctggt tggacaatga atataggtat
     1081 ttattacact tataatcctg ttgcaggtac gtattatgtt gttcaaaata tttaataaaa
     1141 cagcacaaag agatttcttt gtgctgtttt attattttta gttgttgaca aacactaaaa
     1201 tataaaatat aatcaacaca taaacgaacg tatgttcggc aagaaagcag gggattttta
     1261 tggatgagat ggttttaagt actcagaaat ggctaaacaa gaaatacagt aatgttacag
     1321 ggtttgataa ggttccagaa aatgggcgaa cagggtggcc tacaatctat ggacttattg
     1381 aaggactaca agtagaactt ggtattacta atctagtagc taattttgga cctaccactg
     1441 aaaaaatgta cgataatcag gttactccta aatggggcaa aaatctacct aaaaatattg
     1501 tatttcttat tcaaggtgcc ttttggtgta aggggattaa tccaggtggc ttcgatgggg
     1561 tatatactcc atatttagat actgctgtaa aagagttgca gactgatgca gggtttaatg
     1621 gaagcgctgt aactggagtt ttggatgcga aatgggccaa agcgttattt gatatgagtg
     1681 cttttgttct tgttcccggc ggagatagta aaattagaaa aatgcagcaa tggttaaatg
     1741 taaattattt agaatacacg ggaataatgc cttgtgatgg tatttatcaa agaaattcaa
     1801 atcaagcatt aatttttgca ttacaagctg aactaggata ttctcccagt gaagcaacag
     1861 ggtcttatgg taatgggaca actagtaaaa cttatcctgt gtctgaaggt aactcgggaa
     1921 actatgtaag aataattcaa tatggattat atgttaatgg ttattttgaa gacggtacat
     1981 tcgatggtat tttcactaaa tatatgggtt tagaagtact ggcattccga aaatttatgg
     2041 tattgcctga atatactgaa atagcagatg ttgttgtaat aaaaggacta ttgtctagcg
     2101 ctggagatat tagaagatct gcagatggcg cagatacttc tacgcaatta actagatctc
     2161 aaatccaaac attggttgat aatgatataa aaatagttgg tagatatctt actggtactg
     2221 ttggaattgg aaaagatgaa cgaaataagt atctaactac agaggaattg aataatattt
     2281 tttctaaaaa tttatctgtg tttcctatct atcaagatgg aggtgcagca ttagcttatt
     2341 ttacttatga tcgtggctta tcagatggta agaaggctat agatgcagca aaaaatctaa
     2401 atataccatt aacaacagtt atttattttg ctgttgatct agacatgtta ggggaagaaa
     2461 ttcttgcata tgctggagaa tattttaagg gggtatctgc tgtaatgtca tatagcgggt
     2521 atcaaacggg tgtttacgga acaagaaatg tttgttctca aataatcaat aacgggtatg
     2581 cttcgtttgc atttgtttca gatatgtcga ctggttatag tgggaattta ggttttccta
     2641 tgcctagaca atggtctttt gatcaatttg ttgaatttac cataggaagt ggcaacggag
     2701 ctgttggtat cgatttaatc gctacatcgg gtagagatag cggatttaat gagttatcaa
     2761 atgatactaa taatgataat tacattgcca aatacaatca gaaggttata aatcagatgg
     2821 tacgtgcgta tcaatatcta tcacaagctg gtcttgataa tccttggaac cctcatttga
     2881 cattttatag atatgtaaat tattctggtt tgtcatggga catcatttct tctcctgtaa
     2941 cagagcatga tagaaaaatt tatgatgaat ataggaataa attaactaat agcgagggcc
     3001 tgtataatta ttttatagat cctaatactg gttcaataat aggtttgcct cacttaattg
     3061 tgacgttaca atcacagatg tttctagccg acacaatcaa tgatagtgtt tctgactttg
     3121 caggttggtt aggagattta atgacttgct ggggtgaagt taagaaatta ggaatacaaa
     3181 tggagcaagg tgtatttgca ttagtaggta cggctgcagg tggaacattt agtctagaag
     3241 atttatacca agatgttgac gcggtaaatg tttatacctt agcaaaaaga aattctgatt
     3301 caacagcttt atcggcttta aaaacgtact attgtgataa gggtgctatt gatagattta
     3361 atcaatttgt taagaacaga tttggttctt cagataaaat cgcttcagta acaaatagtt
     3421 tattagaaaa tgacgaaaca gttgatatct taggtgtagg gataatttca tttttcattg
     3481 ataaaatagt taataattca cttgggacag attttactta tcttgatttt attggagatg
     3541 aggggaaagt tgcaaatgga ttttctgaaa aaattattca atttcaagaa aatagaagta
     3601 aacttaatta agaatcgttt actatttaaa cagtgtcctt ttccttttta aatatatacg
     3661 ataaaataag tatatgaatt gtaaaaggac atgatgatta tggaagaaat agaatggaat
     3721 aatctgactc aaaataataa aaataattct ggatgtggaa ttatctttat gtttgttata
     3781 gttttgggaa tagtagtact tttaataact gtcaagatag tagattactt tgcgaatact
     3841 cctgaagaac aaatacagag aaaacttcct gagtttgaaa gagtaccacc ttatgatcca
     3901 agagataatt catttcaagt aaaaaaatta aaagagtctc caaatgaaat gtggtttgaa
     3961 gttacagttg aatcaagcga cttcaaagaa aaaaagaaag aatacaatta tattgtaaag
     4021 gcaaaagtat caaaaagatt ta
//
LOCUS       DASJST010000193         3975 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJST010000193 DASJST010000000
VERSION     DASJST010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.196"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(103..354)
                     /locus_tag="U4B05_002713"
     CDS             complement(103..354)
                     /locus_tag="U4B05_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051800.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(368..559)
                     /locus_tag="U4B05_002714"
     CDS             complement(368..559)
                     /locus_tag="U4B05_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051801.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            852..1172
                     /locus_tag="U4B05_002715"
     CDS             852..1172
                     /locus_tag="U4B05_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051802.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1190..1612
                     /locus_tag="U4B05_002716"
     CDS             1190..1612
                     /locus_tag="U4B05_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2051803.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            1695..2330
                     /locus_tag="U4B05_002717"
     CDS             1695..2330
                     /locus_tag="U4B05_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEN2051804.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            2448..3659
                     /locus_tag="U4B05_002718"
     CDS             2448..3659
                     /locus_tag="U4B05_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2051805.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
BASE COUNT         1461 a          644 c          696 g         1174 t
ORIGIN      
        1 caagccctat aaaaatccaa accatgtaca caatcgttcc tatcaacgct tgtctatttc
       61 cccaaagccc taaagcgtag atgattagcg gtgcgctgaa tactagtgct ctgttgaatt
      121 tgcccatgtt tttcctcctt ttagatactg atacaaagcg atactactaa acctccactc
      181 tctgccaact ttcgctgctg gaatctttcc agattcggca tctttagtta gcgtgcgtgt
      241 cgtggttttc aaatattccg cagcttgctt tgtatcccac acttcatttg caatctcaga
      301 ttctgccaaa gaagctttga gggctgagag gttaactaaa gctagttgtt ccattgatta
      361 attcctccta tcgaattctg taatcacgga tgatttctaa gatagtttta ttagcttttg
      421 gtccactcca atggccatca ataatttgct gcattcgaac acgtgtgtat ccatatgcag
      481 tagccaaatc ttccatagtt actccgtttt ctcgcataaa cttcttgatt gcagcacggc
      541 cgttatctag atttgacatc tattcactcc ccttacatat agatttgtaa gtcaaaatga
      601 tagaaaaaac gtataaacta ttgactaata gtatacaatc gtatactata taaacatagt
      661 taaataagcc tacaacaaac cctttattat gcaatcggtc gccaaactta atgctataag
      721 gtgtattttt agtttgcttt ttttctatca aattaactta caaacaaata ataatacaaa
      781 cttatactta tgtcaacagt ataatttaca ttttgtgtac ttttatttgt ttaggattgg
      841 agaacattat tatgacactg tttgaaagga taaaatcatt agctagccaa agagataaaa
      901 gcatgaaaga agtcgcttta gaattaggat ttagcgaaaa tcttttctat cgatggaaaa
      961 caacagaacc caaagcaaga gatttgcaaa aggtagctga ctatttcaat gtctctgtag
     1021 actatcttct aggtagagaa gaaaaagaaa cgcctaaaca tgtggattta tcagaagatg
     1081 atactgtatt ttcttttgat ggaaaagaaa tatctaagga gacaatgcgt aaagcgattg
     1141 caattgctaa agctttagag gaaaatgaat agttggagtg atgggttgta tgtatttaaa
     1201 gttgaaagaa atgctgagtg agtataattt aaagttaatc tatatggaaa tggaagaacc
     1261 aggtttttat tatccaaaac caagaatagt atttttgaat gaaaaactac acgaagacag
     1321 ttctgaagct tttcatttag cccatgagct cggtcatttc attgcttcac attttgaata
     1381 ttcagtactg tacgataact ctacaacttt tcattcaaaa ttcgaagctg aagctgatag
     1441 aatcgcaatt atgattttac taaatatctt tattgagaac gaattgactg atgaatctca
     1501 gttcaaatta gaaaatttca tggaattcta ctctatcaat aataagttaa gaacagaatg
     1561 ttttaatgtt tgccagtcat atttcaagaa aaaatactct tatgcacagt aaaaaaagcc
     1621 cgtgctgcaa cacggactca tacctcattt ctgagatcac aaatatatta taacaagaag
     1681 tgagggatat ttaaatggca aaaaaagtta tgggtcaaga tgggaaaatg tataaggtta
     1741 aaaaaccgtt ttataaacgg gtatggtttt gggtattaat agtacttttg ttttttggta
     1801 ttggtggtgc tctaagtggc ggtgaaaaca aagattcagc tactgcaaca tcaacaagta
     1861 ctaataaaga aactacaaaa gaaactacaa caaaagaaga aaagacttat aaaatcggtg
     1921 atgacgttgc tgtagggaaa atggaataca aagtaaactc cgttgaagta gtaaaacaag
     1981 ttggcccatc tgtacttcct actaacgcaa aagacacttt cttagtagtt gacttatctg
     2041 taaaaaatgc aggtgataag gctgtgactg ttgatagctc attcttcaaa ttaaaagcag
     2101 atggaaaaac tttcgaagct gattcggctg catctatgtc ggcaaatcaa gatgaaaatg
     2161 gtaatatcac taactctttc tttatggaaa gcttaaatcc tgatatgcaa caaactggta
     2221 aaatagtttt tgatatctct gaagcccaag caaatgcaca aaataatgta ttgcaagcac
     2281 aaacaggtta ctttggaaca gagacagttt caattgctct tcataactaa ttagtataaa
     2341 aaacacgccc caccgaccaa agcgagcgtg ttctaagaaa aaacaaacct acacaatagg
     2401 cttctttata gtacctattg tatcagagaa agagagctga ttcaattatg tcagaagtaa
     2461 caggatactt agaacaagta gacaatgaaa catatagact aagagctgta ttagggtaca
     2521 aacctgatgg ctcggcaaaa agaaaaagca aaactataaa agcgaatagt aaaagagcgg
     2581 catataaaga attgaacatc tggttagagc aattcgaagg aatgaccgat gattcgttgg
     2641 atttattcaa tataactttt ggtgaatttt atagaaaaat atggctttca gaggccgaaa
     2701 aaaatttaga gccaaagtct tatcataact ataagcgaat gatcgagaat agatttcttg
     2761 ataaatttga ttttatccct ctaatcgata taaaaccata catgataaag aaaattgttg
     2821 ttaatgctca aagaatcaat acaaaagatc ctggaagaaa ctctgataaa cctttatcaa
     2881 gaaatacaaa gcttcgtatg ttatacgccg ttaacaactt atttttgatg gctaaaaatg
     2941 agtacggagc aattaaagaa aatcctgtgg aaaatgtaaa aataccaaaa gaaaaaggcg
     3001 taaaaaagaa tatcgaagaa ccttattcgg aagaagaaat tcatgcaatg ctaaaagcag
     3061 catttgagga aagcatagaa attaaaactt taattgttct agcatttatt acaggagcaa
     3121 gacaaggaga aattgcagct ttagaagaaa aagatattga ctttgacaaa caagaaataa
     3181 gatttcatca gcggatttca gaagtggatg gaaaatcaga tattcggttg ctgccaggtt
     3241 tgaaaaatga tgatgatgaa aaaatagtaa ccggtcctgc ttatctattt gatatgttag
     3301 atgaattgat caaagagaat aaaaaaattc gttggaaatt aaatataaag aaattaagac
     3361 actattttat atttgataca aaacaagacg gaactttacc acgtggaagt tatctgtata
     3421 agaagttcaa acgattcacc aaacgtcaca atttgcgtca cattcgtttt cacgacatta
     3481 gacatacatc agctacctat ttgcttagcg atcctaatat gactccaaaa gagcttcaaa
     3541 aacgattagg acaccgagat tttaatacta caatgaatgt ttatggccat gtcttacgca
     3601 aagaaaaaga tacagcaact acagcgtttg aaaaactatt aaaaaaagat ataaaataac
     3661 actaaaaaat tatattttcg ggtataaact cgggtaggat atataaaaac ttgggataat
     3721 ttgcggtata taagcattta aaaatgctgt aaattcgctt atcttcattt aaaataccat
     3781 taaataacga actcttaatc agcgggtcgc gggttcgagc ccctcacggc ccattgggtg
     3841 ccaaacccac ggaacaggta aatgttggtg cgaaagcatt tcgaaaacat ttacgctgtt
     3901 ctttttttat tttcgccgtt tgtataatta agcgagacaa ataaaaaagc catttatgtt
     3961 acactcaaat tgtaa
//
LOCUS       DASJST010000194         3925 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJST010000194 DASJST010000000
VERSION     DASJST010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.197"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(573..1130)
                     /locus_tag="U4B05_002719"
     CDS             complement(573..1130)
                     /locus_tag="U4B05_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051806.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="U4B05_002720"
     CDS             complement(1309..1920)
                     /locus_tag="U4B05_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051807.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="U4B05_002721"
     CDS             complement(2033..2290)
                     /locus_tag="U4B05_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051808.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="U4B05_002722"
     CDS             complement(2887..3165)
                     /locus_tag="U4B05_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051809.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="U4B05_002723"
     CDS             complement(3199..3450)
                     /locus_tag="U4B05_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051810.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DASJST010000195         3902 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.198, whole genome shotgun sequence.
ACCESSION   DASJST010000195 DASJST010000000
VERSION     DASJST010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.198"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..381
                     /locus_tag="U4B05_002724"
     CDS             46..381
                     /locus_tag="U4B05_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051811.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(638..2221)
                     /locus_tag="U4B05_002725"
     CDS             complement(638..2221)
                     /locus_tag="U4B05_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051812.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2343..2927
                     /locus_tag="U4B05_002726"
     CDS             2343..2927
                     /locus_tag="U4B05_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051813.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2938..3183
                     /locus_tag="U4B05_002727"
     CDS             2938..3183
                     /locus_tag="U4B05_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051814.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3189..>3902
                     /locus_tag="U4B05_002728"
     CDS             3189..>3902
                     /locus_tag="U4B05_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051815.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSK"
BASE COUNT         1377 a          527 c          624 g         1374 t
ORIGIN      
        1 acaatttcct aggaaaaata gttaacataa aaggaatgaa ttgttatgaa acaatattta
       61 aatgaatgga aagcgataga ggggtcatta gtaacgcaaa gaataaagga attacctgat
      121 tgcttagaga aggatcattt gtttcaaata cgagaaatgt tgaaaaaaga acagtttgat
      181 ccagaccaat ttttagtagt cgagtatcca actattggag tgtactgttg taaccatatc
      241 aatgatggaa aatattttat tattcaggag tatggaggac agttaactcc tttctatact
      301 acatgggaaa tgagtgaaga aggaatcaat aattttccat gtgaaagcat cgaagattca
      361 atttcacaca ccgaatgtta attgattata actataaaaa aggattgaac attatgttca
      421 atcctttttt caatgaaatg ttttccgcca tttcattgtt tggtgcgatt tctattttat
      481 tatatcaaaa taaagagaga ttctaaagaa agaagaggga aatatataaa tcaagcgaca
      541 gtatatttat tattaaatgc tattgtgaga atataactaa ttagtttaat ttgaaagtaa
      601 atatacgcta taaaagtgca tcttgtgtta taaaaaatta ttttattttg tgaacataat
      661 ttaacaattc tttctcggaa ttgatttctt ttcttaaata gtcttcagac ttattaataa
      721 tattatctat tatgtctaga tacatatcac ttttcgattc agctaattca tacagttttt
      781 ttaattcttt atcctttaat tgaaaaccct gatccatagt cactgcattt ggatatagaa
      841 gcccactata atgtttacgt aaattcactt ggaatttctg attataaaag tctaacagat
      901 tagaaataaa aggcggaaaa ttactgtttt tgtaaaattg attatttcca tttaggttgt
      961 atagtaattt gttaaattgc tcctgattta atttattgta aatccatggt aatacttcca
     1021 tatccttatc tgctaacctg aatgaactag catcggtaag atgttcgatc ataaaatcta
     1081 tgaacatctc tttttcacca aaatataaat attgattata tattgtttct aatgttgaag
     1141 atggtagcga agaaactctc attttttgct ggagtatagg atcaactaga ctatttaagt
     1201 gattttgtaa ttcttcattg ttgtttccta gaactgcaag ggaacgtaaa cggtagtaat
     1261 attctatttc tgttagtact tgttgtttcg gtttaaaatc atttattgat atttgtaaat
     1321 tttgaattgt ctcgtctgaa aaatcatgtt gtaattctgg atgggtagct ataaaagtaa
     1381 gtaaaaagaa cccttttgta tacgtgatct gttgattttc accaagtgat ttattaattt
     1441 cctctcttga ttcagctgtg aacctatact ttgcaatttc tttttttaga atatttaaat
     1501 agaattcttt attatcaaaa attagggcat taagtagtct gaaagtactt tccctatctc
     1561 tatctgcttc ctcatttcca taagataatt gaaaaacaaa cttaaaggct gtcttacata
     1621 ggttttcttt ggctttttcg cttagtttat ttagatagct attttttagt gcagagtgaa
     1681 attcttcagg tgtaacagac atagcatgta tgcgacttgc atattgtata atttcgatcg
     1741 ttactttttt tcctaatatt ggatttttta ggaatataat ttcataggac ttccgtataa
     1801 catcctgaca agtttctttt gttatatctc ttaattccca agtattaccg ttgtaagtga
     1861 ctacaggatg agctgaataa tttctttgat ccttaatata ttttaaatcc tcatattcat
     1921 agctatcaat tagctcagtt gaactataag ctttttctag tagttccatt tcccattttg
     1981 gagagtttgg atcacttttt cttagtctag ctatatcttc taaaattttc ttagaagtat
     2041 tatcctcgta aatatttgct aaatcagata atttatcaat taaatctaaa ataagcgcac
     2101 tgtaattgac agctattgca gaacgataat ttccctgata aaaactactt ctaacttctt
     2161 ctaataatgg aataattcta ggatttttaa ctttttttat ttcatcatct aatataaaca
     2221 tataaatctc ctttttttat ttgtatgaca agtttaccat aaaaaaaaat taaaaagcga
     2281 gacatattta tgttgttgtg ttatcataat aacataaata tgtagagtga gatgatgaaa
     2341 aaatggattt gaaacaatcg ttttacatct ggttatatcc tgataatcct atgtcaataa
     2401 tggctgatat ttttgtagtt agcatcttat tgtatttatt ttatctaatt ttctttcaca
     2461 atttaatcgg gtacatgatt cttgattgga aaggaatgtt cgatatagaa agaaaaccat
     2521 ttgctaacaa tcaaacctat accgtcttcg aaagtggaga ttgggaagaa aatgaagttg
     2581 atgtgatcca tgaaattgga ttagctttat caggaataca agatgctgat gttttggtat
     2641 caatgacaac ggatactgga tacaacgctt atgaggtatt ttatcttatg tattttatat
     2701 ggcgtgatgg tcaagaaatt atcaatggtc gtgctgaaaa aacaatcaat tctaatactg
     2761 aaatttcttt ttgtatgggt gaaggggaaa tgattgtata taaagatggg tcagcaaaca
     2821 ttaaaacagc ctttggtgat attgaaacaa gtattaagga acttagggaa tatgtgcaga
     2881 acccatataa ttttcaatat ttagcaggaa gaataaattt agtttaggag taaaaatatg
     2941 aaaaaaatta taaatcaacc aacaaatcac ttgataattg aaggtgttat tttcgtaaca
     3001 atgtatttta ctgtaggaaa tggcatctca aatacaatca ttcaaaaaaa tccacaatac
     3061 ctattattta gtctttatgc ttggattgta caaatggttt gggtttccat attgatgctg
     3121 ttgttttatt ggatagtacg ggttattctt ttattgttaa acaaaagaaa aaaggatagg
     3181 tgaataaaat gaaatcaact aaattgaagg ttgtattgtt atcgacagca gcattaataa
     3241 cccttacagc atgtggtaca actaatagtg ctaataataa caaagacaca aagtctagta
     3301 gtacgatggt tactaaaaac agcactacat cggcatcaac aacaatgtct tcaatgactg
     3361 aaaataatgc tgaaaagtat caaaaatacg aagacattaa taaaggtcaa aagccggatg
     3421 aagtttataa caactttatt aatacagtaa aaaatgatga aaattatggg aatgatccat
     3481 tgcaaaataa aaatctgact atcgttcttt acaaacctga ttgtccagat tgtcaaaaag
     3541 cagaatcaaa aatcatgcaa gaaataaaag aaggatttag ttatgctcca acattaatgc
     3601 atgatattac cataaataaa ccagcttacg agtattacgc agttgctgta gatattactg
     3661 atggtgtacc tgagtgggta caaaacattc ctggtctggc tgatgagaac aatcaatatc
     3721 atacgccaac agttgcagta gtaacaccaa agaaagttaa cggcacacta tactgggatg
     3781 taatgggaat gacaacaaat acaactgatg aaatgattga aagtacaact aaatattata
     3841 tggatttggg agcatataaa ttatatcaac aatcagagcc tgttatggac gatcaatcca
     3901 aa
//
LOCUS       DASJST010000196         3860 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJST010000196 DASJST010000000
VERSION     DASJST010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.199"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..953
                     /locus_tag="U4B05_002729"
     CDS             <1..953
                     /locus_tag="U4B05_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051816.1"
                     /translation="KIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYA
                     YYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTA
                     RTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIF
                     NPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIY
                     KDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCN
                     IEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            950..2371
                     /locus_tag="U4B05_002730"
     CDS             950..2371
                     /locus_tag="U4B05_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN2051817.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2407..3123
                     /locus_tag="U4B05_002731"
     CDS             2407..3123
                     /locus_tag="U4B05_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2051818.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            3142..>3860
                     /locus_tag="U4B05_002732"
     CDS             3142..>3860
                     /locus_tag="U4B05_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEN2051819.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFV"
BASE COUNT         1420 a          452 c          709 g         1279 t
ORIGIN      
        1 aaaaaataga agaaaaaaaa gtgtttgatg gctgtcatta tttagaaagc aaatcttttg
       61 atgataatat tgggtatttt caaaagatta aggactgtct tcagatttct tgtggtaaat
      121 caaatcgata tgcctattat ttgaaaaact gtaaaagtga atattttgat gaatttattt
      181 gtttttcaat gggagaggtt tggttggaag gaatatatgc ttgcttatat cgaaataata
      241 aaaatgtaca ggtatcaatt ttagaagaag gtattttaag ctatggagtt gacattattc
      301 caacagcaag aacaaaagct atcgcagtac tcaattactt aaaaaaaagg actccactat
      361 ataacaaaag ctataatttt tattgttttt ttcctaatat atatacagga agagagaata
      421 caatacaaat tccattagtt acagctgggt caagaaccgc attgcaactt tcggatattt
      481 ttaaccctat gatagatatt tataaagaaa aaaaatatat attttttacg tctgtttatg
      541 attttgaggg cggagaacct gtcggagaat atgaattagt atgcaaaata gcagacttag
      601 taggaaaaga taatctaatt gttaagacgc atcctagaga tgtaagaaca atttataaag
      661 ataatggatt tatcgaggat aaaaattcat caataccttg ggaggcgatt caattgtcag
      721 gcgattttaa cgataaaata tttatgacaa ttaattcagg aagtgttatt tcaggtagta
      781 caatgtctgc aaaacctgta cgcacgtatt atatgtataa attgtgtaac atagaaggaa
      841 atccgtcttg caaaaagaat gcgtatgata ttgagcgatt gcttaagcaa aaaacgatga
      901 aaaacatttt aaagaatgtg aaaatagcag aaagcttaga tgatatacta tgagcaaaac
      961 taaatattta attaaaaata tgggaatatt aacaataagt aatttttctt caaaaatttt
     1021 agtttttttg ttagtccctt tatatacaag tgtattatcc acaacagaat atggcacata
     1081 tgatttgaca atttcaacag ccacattatt gtttccaatt ttaaccttaa acattgtaga
     1141 tgctgtgatg cgctttttaa tggataaaga taaaaataaa aaaaatatag catctatagc
     1201 gattaaagtt atatttttaa gcgtgatagt ctttggatcc gtaatgtatt tgttaaatat
     1261 tacaaaatta tggcaagatg tttatggatt agaaaaatat atttttttgt attatatatc
     1321 ctatgtatgg aatcaatttc taatacaatt tgctaaaggg ttagaaaaag ttaaagctat
     1381 gggcatagca ggtattatca gcacaattgt aacaataatt gccaatattt tcttcttgct
     1441 tattctgaaa tggggattaa ttggtttttt cattgcaaat attttatcac agattatttc
     1501 agctacttat ctgtcagtat tagtgagaat atgggaatat atcgattttg aggctaaaga
     1561 tcctcagcta aagagagaaa tgctaaatta ctcgattcca ttaattgcaa ctgttgttgg
     1621 atggtggatt aatagtacag ctgataggta tgtggtttcc tttatagtag gtgtctcggc
     1681 gaatggctta ttgtctgtgg catataaaat acctcaaata attaatactt tgcagggaat
     1741 atttatacag gcctggcaaa tttctgcaat taaagaatat ggagataatg agactgcaaa
     1801 attttatgga aatacatttg ttatgattaa tttgttaatg tgcataacct gttcaagtct
     1861 tatcatatta actagaccgt tttcacatat tctttatgcg aaagactttt atgaagcgtg
     1921 gaaatatgtt ccatttttat taatagctaa tgttttaaat tgtgcatcag gtctgttagg
     1981 acctatctta tcagcaaaaa aagattctaa atccatgatg tggtctgcaa tcataggggc
     2041 attagttaat gtgattatga gtgtagtcct tgtttataag ataggggttc aaggtgccac
     2101 aatagccact gttatatgtt catatgtaat atatgttgtt cgtaaacgag ctgtgggaaa
     2161 agatattttt ataaagcatt atcatattgt tgtggaatct tggggattat tgtttgttca
     2221 ggcaatcata gaaatatatt ttaaaaatta tgtaatagaa attataatta tgattatttt
     2281 aattattata aatatcagtt atattaagca gttgtgttat agaggaagaa acattatttc
     2341 aatgattaag agtcgagagc acaaacggta gacgatgtta tttttataaa aagatataat
     2401 ttattaatgg tcgataatta tgaaaggaaa aatattctta aaaaaaagag tataggatgt
     2461 cgtatgaaaa tagtcgctat agtaccaatg aagttaaata acaaaagatt gccacaaaaa
     2521 aacacgaaag cttttaccaa tggaaaacca ttgtgttatt atatactttc aacactttta
     2581 aaagtaaaac agattgataa agtgtatgtc tattgtagta acccagatat taaagaattt
     2641 ataccagaag gtgtaacatt tttaaagaga tctagttcct tagatcagga tacaacgagt
     2701 attacacagg tattagccgg tttcacaaaa gaagtagagg ctgatgtata tgtaatgaca
     2761 cacacaacag caccatttat tagcgaaaaa tctattgaaa aaggactaga tgccgtgctg
     2821 acaggagaat atgattctgc atttgctgca aaaaaattgc aagatttctt atggaaagac
     2881 ggtgaaccat ttaattatga attggacaat attcctagaa cacaggattt gccgccttta
     2941 tacgaagaaa ctagtggatt ttatatttat aatcgcaatg tgatgaccga gttgcataga
     3001 agaattggga aaaaaccttg catagttgaa gtaggtgaga ttgagtcagt tgatattgat
     3061 gaagccgagg attttatgat tgctgatgcg atttataatc atcttatttt acaaggtagt
     3121 taagacaggg ggaaggttag gatgaacaat gtacagattt tagattgcac acttagagat
     3181 gggggctatt gtaacaattg ggaatttgga tttgataata caaaaaaaat tatttctgga
     3241 cttatagaat caggaattga gattatagaa tgtgggtttc tcacaaataa aacagtatac
     3301 aataaaaatg ttacaaaata tacgacctta gaagaattag cacagataat tcctgaaaaa
     3361 agagaaggaa aattatttgt agcaatgatg aactatggcg aatataatat agaggatctt
     3421 ccagattatg atggttcatc tattgatgga attaggattg cgtttcataa aaaaaattgc
     3481 aatgaagcgt tgaaattatg tcgtgagata aagaaaaaag gctacttagt ttttgttcaa
     3541 gctatggtat ctctcaatta tacagatgaa gagtttttag cagtaataaa aaatgtgaat
     3601 cagtttaaac catacgcttt ttatattgtt gatagttttg gaatgatgaa aggaaaagat
     3661 ttaactcgtc ttttttacat gattgagcat aatctagaaa aagatataca gataggtttt
     3721 cactcacata ataacatgca gcttgcatat tctaatgcac aaaaacttat aaccgtacaa
     3781 acaaatcgaa gcctgattat tgattcttcg gtgtacggaa tgggaagagg agcaggtaat
     3841 cttaacacag aactatttgt
//
LOCUS       DASJST010000197         3781 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJST010000197 DASJST010000000
VERSION     DASJST010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.200"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..478
                     /locus_tag="U4B05_002733"
     CDS             11..478
                     /locus_tag="U4B05_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051820.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="U4B05_002734"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="U4B05_002734"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN2051821.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="U4B05_002735"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="U4B05_002735"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN2051822.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..3697
                     /locus_tag="U4B05_002736"
     CDS             3200..3697
                     /locus_tag="U4B05_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN2051823.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1242 a          602 c          819 g         1118 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
     3661 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
     3721 aagagaaatc aagaataaaa agagccggaa agacagtggg catttctgtt tttcggtttt
     3781 t
//
LOCUS       DASJST010000198         3770 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJST010000198 DASJST010000000
VERSION     DASJST010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.201"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..444
                     /locus_tag="U4B05_002737"
     CDS             <1..444
                     /locus_tag="U4B05_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN2051824.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="U4B05_002738"
     CDS             444..1643
                     /locus_tag="U4B05_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2051825.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="U4B05_002739"
     CDS             1659..2303
                     /locus_tag="U4B05_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051826.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="U4B05_002740"
     CDS             2314..3120
                     /locus_tag="U4B05_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051827.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="U4B05_002741"
     CDS             3142..3372
                     /locus_tag="U4B05_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051828.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         1396 a          587 c          784 g         1003 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaatttata aagagcataa ggaaacgtgg gaacactttt taaaaaaagt
     3721 gttccaatag gctgtgccta ttacgtttct caaaaataat tgtgggaaca
//
LOCUS       DASJST010000199         3667 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJST010000199 DASJST010000000
VERSION     DASJST010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.202"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            29..781
                     /locus_tag="U4B05_002742"
     CDS             29..781
                     /locus_tag="U4B05_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2051829.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="U4B05_002743"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="U4B05_002743"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN2051830.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="U4B05_002744"
     CDS             1710..3641
                     /locus_tag="U4B05_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2051831.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1177 a          659 c          842 g          989 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgcagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc ttttgaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggat ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagacactga aactgagatc
     1081 aatggcttag gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccagctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cgttgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaacaca accttccaat ctgtagaaga aatcattccg
     1381 tatgggaaaa aattactagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgctggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctttg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagttgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg aatcaaatta ctggctcctc acggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagtag cagaagcata gaaataaaaa agaagaggct
     3661 gggtttt
//
LOCUS       DASJST010000200         3620 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJST010000200 DASJST010000000
VERSION     DASJST010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.203"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(227..1114)
                     /locus_tag="U4B05_002745"
     CDS             complement(227..1114)
                     /locus_tag="U4B05_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2051832.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="U4B05_002746"
     CDS             complement(1111..1980)
                     /locus_tag="U4B05_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2051833.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="U4B05_002747"
     CDS             complement(2117..3403)
                     /locus_tag="U4B05_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2051834.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1113 a          753 c          617 g         1137 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt
//
LOCUS       DASJST010000201         3522 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJST010000201 DASJST010000000
VERSION     DASJST010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.204"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(260..964)
                     /locus_tag="U4B05_002748"
     CDS             complement(260..964)
                     /locus_tag="U4B05_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051835.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1151..1396)
                     /locus_tag="U4B05_002749"
     CDS             complement(1151..1396)
                     /locus_tag="U4B05_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051836.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1413..2264)
                     /locus_tag="U4B05_002750"
     CDS             complement(1413..2264)
                     /locus_tag="U4B05_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2051837.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2430..3434
                     /locus_tag="U4B05_002751"
     CDS             2430..3434
                     /locus_tag="U4B05_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN2051838.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1092 a          602 c          683 g         1145 t
ORIGIN      
        1 ctctatcaga aaatatgttt attattagaa ttttttatag ttaaggttag gcatcttaag
       61 aaatcaggca actccaagat atcttcaggt agaagagaag tcgcctaaca ttgtaggatc
      121 ataggtgatt tgttggacag gtcaagctga tatttgagcc tgatgttaga aggaatttct
      181 ttttacttgc tgtagaaatt attatatgac taaaaaggct gggattttaa atcccagcct
      241 tttatgacaa atagaatttt tatcgatcag taatcgctgt cgctgcaaat tgttcgatca
      301 aaggaatcaa agtcaatttg actttgtatt tccgttcgag tgctttttcg ataacgaact
      361 cattttcagt agtcatagaa atagttgcta atttacgagg ttcatcagca tttttttcat
      421 taataactac ttcatgttca ttttgttctg tgtaaaattt aggatactca gcaacggctt
      481 ttgaaaaatc agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg
      541 tgtccgcttt tggtttttcc gttttttctt ctggcacact gactgtttct tctgtaactg
      601 gtgtcgcaac agcttgcgca gttggttcat cgtttttcaa tcgttgatca ttccagtaag
      661 acacgatttc aataagaaga ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac
      721 gaattctctt taatacagta ttgaagttgt catcgattgg cacgttgatc cgtgtatctt
      781 catttgtttt cccagctaat ccgatcgtta agatttgacg gtttttggga tcaagcatga
      841 cttgtaacgg caaaccataa cgtcgcaagc tctcttcttc tagagaacgg aataattgtc
      901 ctcttacacc atccaatgaa ggacggaatt gatttaataa tttttgtttc tgttttttct
      961 tcatatgttc tctccttcgc tcataccctt tcgattataa gaaaaaaaca gccaaatgaa
     1021 aaggagtaat gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt
     1081 tcccgttttt tgaaagaaaa ataaaaaatg gaacggatta tgtatatata cataatccgt
     1141 tccattcttt ctattttttc ggtatcaagg caaaaacagg tagttcaggt gaataaatga
     1201 cttcatgttc gtgccagggt ttgaccatta gcgaatgatc gaaccgtttt ttcagttctt
     1261 cgatgatttg atcatgtgtc cagtttcttg caggaaaatg ttggattttc tctgcatcga
     1321 tcaaaaagac aaaatcggaa tgaaaagtct gggtaacttc ttccgagaga gaattcaagc
     1381 gttcgattgg atccataaaa tcacctttcg acttaaaatt gggtagtatc tgggtctttt
     1441 gctttgccga ctacaccatc taactgatcg atttgtttca tttcttcttc tgttaattca
     1501 aaatcaaata attcagtatt ttctttgatt ctgcttggtg tcactgactt aggaagagga
     1561 aggaattcgt gttgcagcga ccaacgcaaa gcaacttgtg caatcgtctt gtcatgtgct
     1621 tcagcgattt gtttcatttc aggcacatcg aagattttac cagtgcctaa tggactatat
     1681 gcttcgagga tcatatcatg tttttttgca tactcaacta cagttgtctg taattcgcct
     1741 ggtgcaagga aaacttgatt gaccatcggc ataatttttg ctgtttttgc tagtgtatct
     1801 agatggtggg gtaagaaatt gctcacaccg attgctttga ttttacctgc ttcatacagt
     1861 tcttcaaatg ctcgccatgt atctgcattt gcttgttccc aattatcacg aaaagcaaca
     1921 gggtttggcc agtggatcaa aaacagatct aaataatctg tttggagctt cttcaatgat
     1981 tcttcaaaag aagacatcac aagttcatag ctgtggtttg cattccataa ttttgttgtc
     2041 aagaatatct cttctctagg aattccactt tccttaatcg cttggccgac gctttcttca
     2101 ttcttgtatc cttgggcagt atcgatatgg cgataacctg cagccagcgc ttctttaaca
     2161 gaggaaacag cgacatcccc atcaggtgtt tgccatgtgc cgaatcctac taccgggatt
     2221 tcgtaaccat ttgatagacg ataagtgctt gttaatgaat tcatactttc agctcctttt
     2281 ttattcgttc aactataagt gtagcgcatt tttcgaaaaa aagaatccat tttgtttcta
     2341 ctcttgaaat ttttgtttaa aagaaaatga ttttgattct ggtatttttg attttttttc
     2401 cgtaaactaa gacagaaggg aatgagaaaa tggatatcta tttaaaaaaa gcaattctgc
     2461 atattattga cagagaaact ggttctccag tcttttcaca aaaagagctg gatctgacaa
     2521 aagaatatat tcgtgatttt cttcaaaaga agatccaaaa aatctcttct gctcaaacaa
     2581 aaactggaca actagcagaa gaaagcatga tcgttgaatc acttaaaatc gctcaagaaa
     2641 attttgtgga agcaagtgaa gcaatcgttc agcggtggtt cgatatctac caagaaagtg
     2701 aagaagcacc tagtgcagat gtatttgttg tcttgtatga attagataca gtgatgtact
     2761 tatctttatt gaaagtcaat tatcgtgagg cttacactca ctttgttgaa gcagacgaag
     2821 cagggatcga caaccgcttg attttgaatc gagcaattct aggggcgaaa acacaaaaag
     2881 cagatgaagc attcgctgtg aatttgactg attggaccta cgaattaatt gaaaaaaaat
     2941 atgagttttc tggcaaaaaa gagctttatt tttctggaag agtgatcgaa agtgttcctg
     3001 caccttcttt agaagaaaat gtaaaagtga tccaaaaagt agccaagcat cttggaaaaa
     3061 aatttgagac agaagaattt gatatcatgg ctgatttgaa agaagctgtc tatgatacga
     3121 tagaagaaaa aggtcggttg gatcatgaga tgattgcgga aaaagtcttc aaggaaaaca
     3181 tcacggcaaa aatggctttt aaagaagaag tacaagagca aggatttgtc ccagaagcac
     3241 ctcctgtaaa agaagtacaa gaaatttctg agaaaaaatt tggaaaacaa aaattcaagc
     3301 tatcaaacgg gatcgaactg atcgtaccag tagatgtata tcgcaaccca gatttgattg
     3361 aatttgtgaa taatccggat ggtacgatat ccgtgatgat caaaaatgtg gatgaagtat
     3421 tgaatcgttt ataaaaatga ctagaatatt aggtgttcaa gtaccagtga tcagttaaat
     3481 gaattgtgtc aagagaagga tcggaaaaga cgaatataac aa
//
LOCUS       DASJST010000202         3509 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJST010000202 DASJST010000000
VERSION     DASJST010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.205"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(279..764)
                     /locus_tag="U4B05_002752"
     CDS             complement(279..764)
                     /locus_tag="U4B05_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN2051839.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    854..1199
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1244..1885
                     /locus_tag="U4B05_002753"
     CDS             1244..1885
                     /locus_tag="U4B05_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2051840.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1904..2539
                     /locus_tag="U4B05_002754"
     CDS             1904..2539
                     /locus_tag="U4B05_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2051841.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2557..3372
                     /locus_tag="U4B05_002755"
     CDS             2557..3372
                     /locus_tag="U4B05_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2051842.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1148 a          571 c          746 g         1044 t
ORIGIN      
        1 agtttagtta gaatataata ttttttgtta aatatgactg aaatcaaaag aataaaattc
       61 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
      121 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
      181 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      241 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      301 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      361 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      421 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      481 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      541 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      601 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      661 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      721 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      781 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      841 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      901 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      961 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
     1021 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     1081 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1141 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1201 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1261 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1321 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1381 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1441 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1501 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1561 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1621 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1681 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1741 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1801 cgtggcaatc atctatctta cattgactgg tattctaaca atcgtaatga aacaagttga
     1861 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1921 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1981 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     2041 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     2101 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2161 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2221 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2281 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2341 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2401 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2461 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2521 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2581 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2641 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2701 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2761 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2821 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2881 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2941 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     3001 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     3061 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3121 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3181 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3241 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3301 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3361 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3421 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3481 cttggtaaac ggtgttcaaa agccagttt
//
LOCUS       DASJST010000203         3456 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJST010000203 DASJST010000000
VERSION     DASJST010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.206"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..822
                     /locus_tag="U4B05_002756"
     CDS             <1..822
                     /locus_tag="U4B05_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HEN2051843.1"
                     /translation="PLLYIIDEILNSFYQKSYWGYSLPNYISAVHNAHPNYASYLSYK
                     NTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYMETGKIQEEYMEELRKFLSDKKI
                     LLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSHIDIDYVFLSNLRRFHELPKESR
                     TKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGLMAIRFLADMNVDEIFLAGFDGY
                     SHNEDENYGEQSMEIITKFALLDAMNVQMRDELSELAKKVKVTFLTDPRWVRIDK"
     gene            957..1535
                     /locus_tag="U4B05_002757"
     CDS             957..1535
                     /locus_tag="U4B05_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051844.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1481..1999
                     /locus_tag="U4B05_002758"
     CDS             1481..1999
                     /locus_tag="U4B05_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051845.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            2007..3047
                     /locus_tag="U4B05_002759"
     CDS             2007..3047
                     /locus_tag="U4B05_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN2051846.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            3296..>3456
                     /locus_tag="U4B05_002760"
     CDS             3296..>3456
                     /locus_tag="U4B05_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2051847.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1249 a          387 c          680 g         1140 t
ORIGIN      
        1 ccattattat atataattga tgaaatacta aatagtttct atcagaagag ttattgggga
       61 tattcattac caaactatat ttcagcagtt cataatgctc atcctaatta tgcaagctat
      121 ctgtcatata agaatacttt aacaattgaa gcaatgaatg aaatattcga aatgatggat
      181 gaagaaaaaa aggtatctta tgataaacaa tatatagaag atttatacta caaatatatg
      241 gaaactggga aaatacagga ggaatatatg gaagaactga gaaaatttct ctcagacaaa
      301 aaaattctac tgattgcacc aggaaaaagt tctgtagatg aggtagaaag aataaaagag
      361 tttgttacca aagaagaagt cgtaataatt agtgtaaatt ttgaatattc tcatatagat
      421 atagattacg tatttttaag taatttaaga cgttttcatg aattaccgaa agaaagcaga
      481 actaaatgta ttgttacatc aaatatacaa gcatcggatg tatatttacg gacgagttat
      541 aaaaagttgt tatctgaaag ggaagttgta agagacaatg caggattaat ggcaattaga
      601 tttttagccg atatgaatgt ggatgaaata ttcttagctg gatttgatgg atattcacat
      661 aatgaagatg agaattatgg tgagcaatcc atggaaatta taacaaaatt tgcattatta
      721 gatgcaatga acgtgcaaat gagagatgaa ttgtcagaat tagctaaaaa agtaaaggtg
      781 acatttttaa cggatccacg ttgggttcga attgataagt agtagtaata atgtttttaa
      841 aattttgata aagagatagt gagagaataa aaatgggtaa agtcttaaat aaaaaagaag
      901 aaaaatattt aagggccgat ggggcgagat gaaactctaa ttttgaactt ttaagaatca
      961 tttctatatt gtgtattgtt gcttaccatg ctaatttgcg tttaaattat aatgcagacg
     1021 gaattatagt acatggttca ttaactgcta accagatttt cttgaattat attggtattt
     1081 ggggtcagct aggagttaat tgttttatat ttatttcggc atatttttta agtgagaaaa
     1141 gaaacattca ctttgaaaat gttttgggaa ttgtattaga atgtattttt tatagtgtat
     1201 tagtttatgg aattctttgt gcatgtggaa tagaaacatt ttcattgaac agctttgtaa
     1261 aagaaatatt atcatttgct ctaggtagat attggtttat tacatcctat ttagcattgt
     1321 atattctaag tccgtattta aatatcatgc ttaacaatct ggaaaaactt cagttgcaaa
     1381 aaataagtat gttattgttt ataggtgtat gctgttatcg tatgttattt aaaacatctt
     1441 tgtttgggga ttgtgctgtt ttaattgcac tatatgtcat atgttataca ctgaaaaaaa
     1501 cgataataat atttttgaga gacacagaat cataggaaca attgtatgta taacagttat
     1561 accacttctc atatccatag gaatactttt caaatttata ccaaatgcaa tggttaacca
     1621 gatggttcat attggttctc cattgatggt tatagcaagt atatttttat tttatgtatt
     1681 taaaaatttt tcattttaca gtaagtatat aaataaactt gcgagaacca gtttaggggt
     1741 atatatttta catgaaaatc attatttcat tgatggtatg aagtctagag gcttaatatg
     1801 ggatgggata tttggattag gcaaaaggtt tttagatagg gatttttgga tttggtttat
     1861 aattgcagtt gtagttgtgt gcataatttg catggctatc gatgttgtaa gagaaaaatt
     1921 tgaaagacag atttttaaaa ttaagagtgt aaagaacctg tgtaaactta tgaatcatgt
     1981 tataaatata tacccataat taataaatga ggagaaaaga agtgaaagtt atatcattcg
     2041 gagaattatt attgagactt gcagcacccg gttattcaaa gttatttcaa agagatagtt
     2101 tggagactac attctgcgga ggggaagcta atgtagcggt atccttagca atatttggat
     2161 atcaatcaga atttataaca aagcttccgg attcagatgt tggatatgcg gcagctaatg
     2221 ctttgcgtta ttttggagta aatgttgata atattatatt tggagaggga cgcatgggac
     2281 tgtattattt agaaaaagga gcagctcaga gaccatctaa agtaatttat gacagaaaat
     2341 tttctgcaat ttcgttagct gatagtgatg aatttaagtg gaaggagatc ctaaacgatg
     2401 caaattggtt tcattggaca gggataaatc cagcattgtc tgagaatctc tttgagacgt
     2461 ccttagatgc atgtcaggct gccaaaaaaa gaggtataac aatttcttgt gatttaaatt
     2521 atcgatccaa gctttggaca tcagaggaag caaaagaaaa gatggaacag atactaccat
     2581 tggttgatat ttgtatttgt aatgaagaag atgcagaaaa agtgctagga attaaagctg
     2641 agcatacaaa tattaatagt ggagaattaa ataaagatgg ctatgtgcag gtggctaggg
     2701 agattcacaa gaagtttgga tgtaggtatg tagctacaac tctgcgtaga agttattcgg
     2761 caagtagaaa tggttggagt gcaatgttat acgaagcaga aaaagataaa gcgtattttt
     2821 ccacagaata caacatacaa attgttgatc gcgttggtgg tggagacagc ttttctgctg
     2881 gtataatata tgcgttatgt gaaaatatgt ccggacaaaa ggctattgaa tttgcgactg
     2941 catctagttg tttaaaacat actatagaag gtgattttaa tcgaactaca attgaggatg
     3001 tatataattt aatgaatagc ggtggaaatg gtcgcgtgtt gcgttagtca acatataaag
     3061 ataaatcaca gtaattttta tggtgattat ctgtcttagt agtgattaga ataacgtgaa
     3121 aataaattgt tgagcgttta tactgttttg aaaaataaat ttaacatatt gaaaaataca
     3181 aatatatacg tatattataa agcgccgatt gtaaaattaa gctagacaac taaaacagtc
     3241 atttgtgcta cactcaaaat agttccgacc aaagaattat aaggagtgag tgcaaataac
     3301 ctatacctat cttacaacgg atgaacttgt aataatagag tcttatttga aaattaacca
     3361 atctgttgct aaaactgcgc attgcttgaa tcgttcaagt caaacgatcc ataaagtata
     3421 tctgttcttc aagcaaagaa aatcagcctt agaata
//
LOCUS       DASJST010000204         3443 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.207, whole genome shotgun sequence.
ACCESSION   DASJST010000204 DASJST010000000
VERSION     DASJST010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.207"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            327..539
                     /locus_tag="U4B05_002761"
     CDS             327..539
                     /locus_tag="U4B05_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051848.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(817..2616)
                     /locus_tag="U4B05_002762"
     CDS             complement(817..2616)
                     /locus_tag="U4B05_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2051849.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2740..3336)
                     /locus_tag="U4B05_002763"
     CDS             complement(2740..3336)
                     /locus_tag="U4B05_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2051850.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1255 a          620 c          602 g          966 t
ORIGIN      
        1 taaatggttt agctattttc ttttaatccg ataagtagcc caatcatcaa ggttacaaat
       61 actattgctt gaatataaat catatgctca cctctttata aataacttag caatataaag
      121 aaaaaaacgc acgaacaaca taggaatata aacaaacgtt tttgtcacaa cgtctaagat
      181 cgtgttaaaa aatagttatc taaaaagcag aaagatactc aaaataaaaa ataaaaaaat
      241 ggataaaaac gttttcaaat acaaggtaat ttgttattat aaaataaaga aaaaacaaaa
      301 taaaaataaa gaaaagaggg aatgaaatgg taaaagcaga agtaggaaga tcttatatat
      361 gcaaaccgat cggagtatct catgaactaa tcggctgtat tgaacgtaca tatatcaaca
      421 cggctgtcgt gtatgttgaa agttatgacc aacgagacta tcaacttatc aatgaaagca
      481 agtatcgcat gctcgtaaaa ttctgtgata ttagcgcacc tgctgaattg gtttcttaaa
      541 cagtattttt gagaattcgc aagaactccc ttataatata aataaagaca gaagggagcg
      601 agaagatatg ccaaaaagaa aaagttcagg gaaaatggga attattcttg cagtgatctc
      661 tgcaattcct gctattttag ctattgttcg tgcgatgaag gaatcaagag aaagttaagc
      721 acacattatg agcgtcaaac aaaaaacgca tatacctgcg gtggcaggtt atgcgttttt
      781 tgttgtataa agactttttt cattaacgat ttttgattag ccgactccca aaacaaaatt
      841 tagcagttta tctgaataag tgatttcttc atctgtttgt ttcacacttt catttagtag
      901 tcccatctca tcggttatga taccatccac accataaaac atcatgcgac tcatagcatc
      961 tgaatcgttg attgtccata catatacttc ttttccgtct tggtgggcag aattaatgaa
     1021 gttacggttg atcgtactat attccattgt cagaaaatca gcaggcgtca cagggggccc
     1081 aacaatattg aaaggtaaga tgtagcccac ataaaagttt ggttctgctt cttttaaatc
     1141 tgaaacggtt tggtacgtca aactttgcaa aatatgatgg ttctctaaga taactttgcg
     1201 gtatttatgg acaaatcgtt cgacaaggtc atccgaatct tttttagagg tcttgatctc
     1261 aataagtaat ttttggttta gcttatgtgc tttttccaaa taagaatcaa aagaacaaac
     1321 aggggcttgc actccgtttt cagtaactgt aagatttgtc aattctttca atgtaagatc
     1381 ctgtggtgtt ccatttaccc ctgttaagtg acgaagattt gcatcatgca tcactacgaa
     1441 atgaccatct tttgtttcct gaacatccat ctctacataa tcaggatgat agtctctgct
     1501 tgttttttca agtgcttcta aagtattttg cgccccattt tgggtactca cacctctgtg
     1561 agagacagtc actggttttg tctgaacagc tgaagtcaga taatccatat tgtaaagaca
     1621 gacgccaata ccgaaaaaaa ctgcaaaaag agtcaaacca gtgtttttca gcgcagagaa
     1681 acgcaagttg ggggcttgtg ggataaacca cttagggatc tcaggcaaga agccttcgtc
     1741 atccatgaaa tcaacaatga tataaaaaat ccctacggta gacatcacga tattgaataa
     1801 taaaatccct tgtaaaagcg tcatagcaaa cacagcgctg atcaatgcat agtctgggaa
     1861 aaattgttca accatacttt gtgccaaaat aacaacgata tatcctaaag aagaaaggag
     1921 taaaatcgta ccgccaatca caataaattg gcccgtgatg gccaaaagac gcgatttggt
     1981 caatgaccag ctttcccgaa tggctgctcg aaaaggacga tctcgtagaa tcatttccgg
     2041 taaagcaaaa atcagtcgaa tcccaatata gccaaggaaa atataaacga gtaaaaagga
     2101 actgacgatg atccaacgat tagcaaaaat aaaatccata atgaaagcgg ggatttttat
     2161 ttttgacagt agatcagaat taaaacttaa tccgctgatc ggtagaatca ataaaaaata
     2221 agcaaggaaa aacaaaaaag taatgggtct cactttcttc agctgaagta tcgtcaagtg
     2281 taagagttgc ttcaaagaga ttggttcttt ctttttaata aaaaaaacac tcatcaaaag
     2341 aaaagtaaat tcgaaataaa ctaacagtaa aatcgtcatc aaaactacga ttaacgagag
     2401 tagaacccca ggatgttgag aaaaaattga aggaatattg tcataagaca aatagtcaat
     2461 tgcccctcgg tgtaaaataa aacgggtcgt actgctcaat aagggtaaac aaataaataa
     2521 aataaagcca tgcatcagca agacatcacg gaaataagct tgtgttcctt ttaaaaagtc
     2581 ccaagcatta cctaagctac gtttcaaata attcatccta tcacctgctc ttcacattgt
     2641 agcgcaaatc taatgaaaaa ggaaagggtg cagtgtgttt tgcagaccct ttcccagatt
     2701 taacattgtc aatcaatgac taattttaca aaataaatat tatccaagaa gaccacttag
     2761 atattgacct ccataaatca aggccatact atataagaaa atcgacagtg gtttagcaat
     2821 caaaatgatt agtgtatatc gtttcactga catgtttgtc aatccagcca tcaaacataa
     2881 cgcatcgtca ggagcaactg gaagaaaaat agctagagca aacagcttct caaaacggtt
     2941 ttgattatcc agccagccaa tatatttgtt atatgtctta tcgctaatca tggaaagtat
     3001 gaatggcttt ccataacgac gccctagcag aaaaatgata atcgaaccaa tgcagattcc
     3061 aatatagtta tagataaatc cagcataagg gccaaatatc aggacaccag ctgcagtact
     3121 gatccctcca ggaatgattg gaataactac ttgcaggatt tggatgaaga caaaaataat
     3181 cggtcctaaa atgatggaat cacctactaa tcctcttaac gcattcaaat ctttgaaaac
     3241 acctaaatta ataaaataaa tcgtcagcgc gattgatagg cctaatccga taatggatat
     3301 gaaatttatc agttttcgag aggcagcaat attcattatt catcaactcc ttagaggttc
     3361 tttgttaact atactttact agaaaagcaa ctaactgcgc cattttttga tatgacagaa
     3421 aacaaacaat tttgtaagtt cat
//
LOCUS       DASJST010000205         3409 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJST010000205 DASJST010000000
VERSION     DASJST010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.208"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2303)
                     /locus_tag="U4B05_002764"
     CDS             complement(<1..2303)
                     /locus_tag="U4B05_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051851.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="U4B05_002765"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="U4B05_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          933 a          765 c          550 g         1161 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaa
//
LOCUS       DASJST010000206         3351 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJST010000206 DASJST010000000
VERSION     DASJST010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.209"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..189
                     /locus_tag="U4B05_002766"
     CDS             <1..189
                     /locus_tag="U4B05_002766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051852.1"
                     /translation="DLEKLTKQRDMKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIE
                     DWAIFMAKEGKEMFQNKG"
     gene            211..477
                     /locus_tag="U4B05_002767"
     CDS             211..477
                     /locus_tag="U4B05_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN2051853.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            504..803
                     /locus_tag="U4B05_002768"
     CDS             504..803
                     /locus_tag="U4B05_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEN2051854.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            977..1528
                     /locus_tag="U4B05_002769"
     CDS             977..1528
                     /locus_tag="U4B05_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEN2051855.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1674..1967
                     /locus_tag="U4B05_002770"
     CDS             1674..1967
                     /locus_tag="U4B05_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051856.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1960..2256
                     /locus_tag="U4B05_002771"
     CDS             1960..2256
                     /locus_tag="U4B05_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051857.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2331..3329
                     /locus_tag="U4B05_002772"
     CDS             2331..3329
                     /locus_tag="U4B05_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN2051858.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1259 a          459 c          664 g          969 t
ORIGIN      
        1 gatttagaaa aacttactaa acaaagagat atgaaaaact ttaataattg tgtagttttg
       61 ataattataa ctgaattaac tttatttaca aatatatatt atatccatga agcccaatct
      121 tctttaatag aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat
      181 aaaggataac gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct
      241 ttatgttgga atctaatcga tcaaaacgtt caaaagcaat ttcctttaga ctatcttcaa
      301 atttttgaat tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaaccg
      361 gaagaaagaa aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa
      421 atatgggcca ttgattcggg tgagtatcaa acgatgttat taccagaaga atactagtat
      481 ctaaaagaaa ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg
      541 cacagctggt cactgcttct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg
      601 aaattgaaat gggtatgctt cctgaagatg ttcatctacc acgtttagtg atgacacatc
      661 tttatattta ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata
      721 ttaccagcca gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc
      781 agattggagg aacggatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa
      841 gatatcctta ccgcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt
      901 ttcaactagt attgacactt ataacggcaa tggtaattga agtgcttgaa caaaaaatag
      961 cataggagaa aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg
     1021 agatcctcat catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga
     1081 agcggaagat aaattagcag tagtcattac tattcgtcca acagatacag aaccatttac
     1141 gaatgatttg agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt
     1201 tattgctgtg aatctttttt cgtacacaaa agcgaaaagt cctcgggtat ttcttcgagg
     1261 aaatgatgaa caatctttgg aaatattaac gactgttttt acagaaaaga aaatcaatac
     1321 cattattttt gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcaaa
     1381 gactatttac gatcaattga gtgcgaaaca aaagaagatg accaaagtat tgataaatgc
     1441 agaaggtaag ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga
     1501 tagtcaatta ttatttcaag aaaaataaca ttttattttc tcatgttact tataagtagt
     1561 tttacatgct ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat
     1621 gtacccagta atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga
     1681 ttgatgaaac ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg
     1741 aactggattc agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt
     1801 ttccaatgaa aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac
     1861 aggaaaaaac tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga
     1921 ttcaaaaagt aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta
     1981 ggtggtttaa tttacggtgg atattggtta tatcaatata tgcttgcgca tccgaatata
     2041 gataaaggaa aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact
     2101 aagaaaaata atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga
     2161 agggaaattt ctactagttt taatccatct gagagtcctc aaggaagaga ccgctgccat
     2221 acgggctatc gacattattg ggaaagaatc gattaaaaga ttaccattta gatcaattaa
     2281 ttgtctgata gaataagtac gtgagggaga aattgaaagg gggaaatata atgaaaaaat
     2341 ggctgttatt actaatagga atgggaattt tattcactgg ttgtacatct gagaatacga
     2401 ggactaaagc gactatagaa actgatacaa caattgagac taaaaaaagt aaaacgcaat
     2461 caactgaaca aactacaaca gaaatgaagc aatctgtgga aacaagtgca agttcaatca
     2521 ctgaatcttc ttctgaagag gttaagaata ctttgtggga tacgaataaa gcttccaaat
     2581 tagaaacttt tgtcacacaa tggggaaaga cattaggtca agaatataaa agctacaatc
     2641 ttcagaataa tgtaagtctg tatggcactc ctttaccaca agccgtgatt aatggcgatt
     2701 ggaaaatggc aatcaatgaa gcaccagtta ccgtacaatg gtcagaagat ggaactggtc
     2761 atgcagattt caatttagta gcagtttatt ctgatgtaga aactggagaa tatttagggc
     2821 agcatgttta ctttttcggt tttcaaaatg gacaacctaa agtttattta acgcaacaaa
     2881 atcaaggtaa tgaaaataat tatctgtact ttaacgaaac tcaaaatcaa cagttaaaac
     2941 aaggtttcag tgatattgtg aatggacaaa ctcctcaaac accagtagta gaagaatcta
     3001 ctcaacaagc aacaactaca acgacacaat ctacaagtag ttcaggaatt tcaagttggg
     3061 aagctgctaa agaatctgtg ctaaataata aagctttatg gttgtatgat tctcctgata
     3121 aaaatgtatc aaatactaat ttaattagca caaatgaata tatgaaagat ctaatgcaag
     3181 atgaaaatgg aagctactat tcaattaata ttgtaacgga tgatgatgga aacacgacca
     3241 tggctggtgg aaaacaattt cgtgtttata cagatggaag aatttctcaa cgtattggaa
     3301 tgactaacaa ttttcaaatg attaaataat tgaaaatatt atttatcaaa g
//
LOCUS       DASJST010000207         3240 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJST010000207 DASJST010000000
VERSION     DASJST010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.211"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            205..1173
                     /locus_tag="U4B05_002773"
     CDS             205..1173
                     /locus_tag="U4B05_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN2051859.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="U4B05_002774"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="U4B05_002774"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN2051860.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="U4B05_002775"
     CDS             2203..2811
                     /locus_tag="U4B05_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN2051861.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          966 a          616 c          785 g          873 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaaga tgtaaggcag agcgatattg
     2941 cggtcattat ctgcgtgcgc tgcggcaaga tagcctgata ataagactga tcgcatagag
     3001 gggtggtatt tcacaccgcc cattgtcaac aggcagttca gcctcgttaa attcagcatg
     3061 ggtatcactt atgaaaattc atctacattg gtgataatag taaatccagt agggcgaaat
     3121 aattgactgt aatttacggg gcaaaacggc acaatctcaa acgagattgt gccgtttaag
     3181 gggaagattc tagaaatatt tcatacttcc aactatatag ttaaggagga gactgaaaat
//
LOCUS       DASJST010000208         3221 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJST010000208 DASJST010000000
VERSION     DASJST010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.212"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="U4B05_002776"
     CDS             301..585
                     /locus_tag="U4B05_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051862.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="U4B05_002777"
     CDS             588..1004
                     /locus_tag="U4B05_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051863.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="U4B05_002778"
     CDS             complement(1141..1860)
                     /locus_tag="U4B05_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2051864.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="U4B05_002779"
     CDS             2535..2630
                     /locus_tag="U4B05_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2051865.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="U4B05_002780"
     CDS             complement(2932..3177)
                     /locus_tag="U4B05_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051866.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DASJST010000209         3221 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJST010000209 DASJST010000000
VERSION     DASJST010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.213"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            43..432
                     /locus_tag="U4B05_002781"
     CDS             43..432
                     /locus_tag="U4B05_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051867.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            436..1200
                     /locus_tag="U4B05_002782"
     CDS             436..1200
                     /locus_tag="U4B05_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2051868.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1329..2276
                     /locus_tag="U4B05_002783"
     CDS             1329..2276
                     /locus_tag="U4B05_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2051869.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            2406..3146
                     /locus_tag="U4B05_002784"
     CDS             2406..3146
                     /locus_tag="U4B05_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051870.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1058 a          534 c          693 g          936 t
ORIGIN      
        1 aatgattata atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt
       61 gtgttttgtg ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg
      121 attcaacagc caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta
      181 attttaagcg tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc
      241 agttttttga atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt
      301 tgttctctgt gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg
      361 aacctgttcc agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa
      421 ataggtgagt aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg
      481 ttagaagttg ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac
      541 ggatggcaga caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc
      601 attcgtgtga tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct
      661 attccatcct atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt
      721 atcaagcatt tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg
      781 atggggggaa tgacaacatg cgccttgttg gctaaacatc ctcatatagt agcgggcgtg
      841 tgtttgatgg gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg
      901 gaatataatt atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat
      961 gatttgtcat tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa
     1021 gctgacgaaa aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag
     1081 tcttacggaa aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata
     1141 ccactaatgg aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa
     1201 ttgaaacgga aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa
     1261 taaactctgt taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg
     1321 aagagagcat gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg
     1381 tctctcgagt catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct
     1441 cagcaatggc tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc
     1501 ggtcgcagat catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca
     1561 tgaatttgct gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag
     1621 ttacaaatga tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa
     1681 aaaaagactt cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt
     1741 ctgaaggata tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat
     1801 atgccttaga aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt
     1861 ttgaaatttc aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta
     1921 aaatcttgcg gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg
     1981 cagattttac agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag
     2041 taagagggat tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg
     2101 atggggtatt tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta
     2161 tacgtatggg tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga
     2221 gtcaaggtta tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct
     2281 tattactata tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg
     2341 ctactgaaaa caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg
     2401 tttgaatgaa agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag
     2461 gcgtaaaatt aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga
     2521 ctgaacagaa tcagtccccg tttgaacaga tgaaagaatt tcatttcacg aacacgaaca
     2581 ccgagaaaat ccgtcgcaag cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg
     2641 aaaatttgga aaagcataaa ggaaaattac cggattatac cggccattac caatcagaaa
     2701 tcacatcgac aggacgaaaa aaactttttg gtgattcctc tcgttctgtc agacaaagtg
     2761 aaacaaggac agagtccaag catactacac gaaacgcatc tgttgacacg aaggtgcggg
     2821 agcgttctta ttttgttcct aaatatgtac ccgcatcgat tatcccagac cctagagaaa
     2881 gcaaaatttc tgaggatcat ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt
     2941 tgttcgatac agaaccgacg ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa
     3001 cattcaatcg aacaaaaacg ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa
     3061 aaagccatgg tatcttagaa cgctcattac aaggattgat cgatgatcaa ggaaactctt
     3121 tgggagaaaa tagctatttt aaataagtga tatacaaatt tatattcggc tacagttgga
     3181 aaggaaacgc tgcggcgttt ccttatttta tactctatcc g
//
LOCUS       DASJST010000210         3221 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJST010000210 DASJST010000000
VERSION     DASJST010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.214"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            166..1206
                     /locus_tag="U4B05_002785"
     CDS             166..1206
                     /locus_tag="U4B05_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2051871.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            2053..2385
                     /locus_tag="U4B05_002786"
     CDS             2053..2385
                     /locus_tag="U4B05_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN2051872.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3004..>3221
                     /locus_tag="U4B05_002787"
     CDS             3004..>3221
                     /locus_tag="U4B05_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN2051873.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
BASE COUNT         1194 a          450 c          592 g          985 t
ORIGIN      
        1 taatgacttt aaaggtagta cataattcat tttatttctg attttaggta gtatttaatt
       61 aaaaaacagt tttaatgcgt ttgcattttt tatttatctt tgctattttc gaaaagagta
      121 ggtaatttta cataaataaa acaacgtgaa agtagaggat ctacaatgac tgattttaat
      181 ttttttaagg cggatagagt ttacaacgaa ttattttatc aatttccaaa agtctttatt
      241 gtttctgacg aatacaaaaa aatgaaagat tcaactaaga ttgcctatat gcttttaaaa
      301 gcaagattag agatcgcaat cagcaaacgg caaatcgatg aagaaggtaa tgtttatttt
      361 acttatacga caaatgaact atgtagcgta ttaaactgcc aaaaacaaaa agcgatagca
      421 atcaaaaaag agttggaatc ctttggttta ttattacaaa agcagatggg atttaacaaa
      481 cagttaggga aaaataatcc taatagacta tatctagcag aattaaaagt ctcagaaaat
      541 gatatctact tactcgaaaa atttgataga gagaataggg aaaacgttga taaatcagag
      601 ggtatgaaaa tcatacccac cctcgacgaa aaatcagacg ctgaatccct tggggctcaa
      661 gagggtatga aaatcatacc gtgccaaaac gttgataaat cagagggtat gaaaatcata
      721 ccagaactta ataataatat attagacact aatagacaca atatagacac tgaaaaagac
      781 cgcctacaag atcaattgtt gttagacaat tttgagacaa ttatgacaaa cgacagcatt
      841 gctacgttta tccctgaacg agtattaaat ttaataaaaa cattttcttc aagttacagt
      901 gaagctcaaa aaactgtcca gactattcat aatgcaaaga aaaaagccga aacagaaagt
      961 ggtatttcaa taatttttga agaactcgat agttattatg tcaatgcaga acaagaatta
     1021 tacacgacac tgttaaaagc ctatcaaaaa ttaaaaaccg aaaaagttga aaatatccag
     1081 aacctgattt ttgtctatgt aaaaaattgg tttatcgaaa aaccaatagc tgctaaagta
     1141 tcaagtgaaa aacgtttgaa ttctgaaagc ttcccaaaaa ctattacgaa agactggtta
     1201 gagtgaaatt tcactgacaa attgattgtt gcttcatgga gtagcattaa aattaaaaat
     1261 tttttattat tctaattata ttacaaatgt tacaaagaaa tagctatttt aatagaattt
     1321 atgagttaaa atattatgat aaaaatcaca aacgtagctt tttacgtcat aagtgttaat
     1381 agttaataat taatttccaa cacaaaaaag agaacaaacg tttgcatgtt gctgtttttt
     1441 agttataata atacgcgaag agaggtaatt tcatttttta aaaaagctaa aaaccttgat
     1501 attacttctt ttcaggcaat acgaatatta ttcgcttaca tttttttgta taaatttagt
     1561 tgttttaaat ttaattgtct gctacgcttg tgatgaatta attttcgttc tgcacttttg
     1621 gacgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggccctgtga gactcggaag
     1681 gctggtaacc ttcgtcgacg tccacaggtg gcattaagac aacctttaat cccttaatgc
     1741 gtttaccttt cagtgttacg gtattacccg caacggattt agtataacag atattcaaaa
     1801 gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta
     1861 tgcctaagca taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga
     1921 aattagaggg aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat
     1981 caattctcta tttttgaaaa ataacaagaa aaacttatta aaaatattta aagtaagtgg
     2041 gtggattatt ttatgtcata tacaggaatt ttgagtctcg aagatatttg tcactacggt
     2101 aaacggtgta ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc
     2161 gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat
     2221 tacgctggta aacaaaagca aataatttta aaagatccta tccctttaaa agaactatat
     2281 ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga
     2341 gtcttgcgtt gtaccgcaaa agggatgggg cgtttaatca gctgaattga tcattctcat
     2401 aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaagaact
     2461 tagaattact agaaaaataa ggagtagact tgatctatca acaattcctt tcggggggtg
     2521 acggggtagg tttttacgat tggtaacgta aaaaattggt caatcgagta aatgcccttg
     2581 tttggatagt ggtgaaatga atttgtgtaa ataatatgca cagagtaaac aagagggaat
     2641 ttagaagtta gacaagttag aatagcgcct agattaatca tgagcagcta ctaagggatc
     2701 taggctttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca
     2761 caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tggtcattgg
     2821 atcaatcgag catttaccga tttctactag ggtttctcgt ttcggacgcc ataaacgagt
     2881 cgatattttt tatgtatttt taagtaaacg aaaaacgata aaatatgaac gagtcctgaa
     2941 taaagtgaaa aaattgatat aaaacataga gaaaatgaaa tttacgtaat ggaggaatga
     3001 caaatgaaaa attatgatcc gaatatacga ttgggcacac atactattaa ggtttctttc
     3061 caacgttggg attacaaagg atttgtgacg tttagaagag gcggaaattg taaaggatta
     3121 gatgttttag cattagacga ggatgattta tactatcaaa aattgactga taatccaatt
     3181 ggattcggct tgctacctga agatgatgaa ggtaatgaat g
//
LOCUS       DASJST010000211         3218 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJST010000211 DASJST010000000
VERSION     DASJST010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.215"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..1438
                     /locus_tag="U4B05_002788"
     CDS             50..1438
                     /locus_tag="U4B05_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEN2051874.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3218
                     /locus_tag="U4B05_002789"
     CDS             1776..>3218
                     /locus_tag="U4B05_002789"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN2051875.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaa
//
LOCUS       DASJST010000212         3108 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJST010000212 DASJST010000000
VERSION     DASJST010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.216"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(114..2198)
                     /gene="cadA"
                     /locus_tag="U4B05_002790"
     CDS             complement(114..2198)
                     /gene="cadA"
                     /locus_tag="U4B05_002790"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2051876.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2723..3082)
                     /locus_tag="U4B05_002791"
     CDS             complement(2723..3082)
                     /locus_tag="U4B05_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN2051877.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT          915 a          694 c          492 g         1007 t
ORIGIN      
        1 aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta
       61 tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg
      121 tcctcttttt ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca
      181 acatctgcaa atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc
      241 aaaattttta tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt
      301 gctaaatgga ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct
      361 gcttcaattg cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct
      421 agcaccggtg catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt
      481 aagatttcct ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc
      541 acgcctactt gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact
      601 gtgttcttca caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag
      661 gtatctgcaa taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta
      721 ccgatttctt gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct
      781 gctaatactt gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata
      841 ttagttgttg gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt
      901 gtagaaaact gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta
      961 tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact
     1021 gtctcggttt gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta
     1081 cttgcgcctc cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga
     1141 catgagatca ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa
     1201 acaacaagtg gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa
     1261 tagcgcgcga atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct
     1321 actaattcta ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa
     1381 aatggtttat ttttattgat aaatccgctt aatactgttt caccaacata tacagattta
     1441 gggactgatt ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt
     1501 ccatctaacg gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct
     1561 tcagggttca cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc
     1621 gccatcaatt cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg
     1681 ccgatttgat aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg
     1741 ccaatcgtag caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc
     1801 atcactgcct tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc
     1861 aagcgaattg gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat
     1921 aaaatccgga acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta
     1981 tctatttctg ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt
     2041 tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt
     2101 accgttgctt ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag
     2161 ttcgcacaat ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa
     2221 catatgaaca tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc
     2281 cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct
     2341 tattctttct ttttctcttc gatcattgcc cctgctctta tccaacccgc acttgccttc
     2401 ttgatcttga caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc
     2461 attaattttt cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata
     2521 ttcgaagctc cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca
     2581 actttccata aacagtaatc ccatataaat aaaagacgta aaagtcacat gggagaactt
     2641 atacaggaaa acacatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcgttt
     2701 tctaaccttt attattctct ttttatacaa tttctgcacc gaatggcatc aacgccaatc
     2761 gagcaagttt gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga
     2821 tgccgctgac gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat
     2881 taacgatcaa tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag
     2941 aaagggaggc gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc
     3001 aaagtatccc cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga
     3061 tgacatttcc taaacaactc attgtatttc tccttctatt actgtttt
//
LOCUS       DASJST010000213         3077 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJST010000213 DASJST010000000
VERSION     DASJST010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.217"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..702
                     /locus_tag="U4B05_002792"
     CDS             <1..702
                     /locus_tag="U4B05_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2051878.1"
                     /translation="NSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPV
                     IDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVKADGIYRPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPQR"
     gene            959..1606
                     /locus_tag="U4B05_002793"
     CDS             959..1606
                     /locus_tag="U4B05_002793"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2051879.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            1889..1962
                     /locus_tag="U4B05_002794"
     tRNA            1889..1962
                     /locus_tag="U4B05_002794"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1923..1925,aa:Ile,seq:cat)
     gene            2508..>3077
                     /locus_tag="U4B05_002795"
     CDS             2508..>3077
                     /locus_tag="U4B05_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN2051880.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1096 a          468 c          574 g          939 t
ORIGIN      
        1 aatagtcctg tgcagattga gttacaacac acacatgata aagcaacgtt tgagaaaaac
       61 tacaaggcat acgttgaatt agctagagat tcagcaatga aatatggtat tccacttaca
      121 ctagacacac cgtataatca accagggatc aaatcgcatt tatgggtaac acaaaatatt
      181 tggggcgatc atacagatcc ttacggttat ctttctgaaa tgggcgtaag taaagaaaaa
      241 ttagcctatg atttggctca tggatttacc gatgaaaatc cgacaacttc agatgataaa
      301 ccagtcattg atccaactcg agcaggtgct gcaaatccta ccctgacaga tggaacaaat
      361 tacgcccaca ttgatcagtt cggagaaatc gaaaacgcaa acttacatgt ggctggttgg
      421 cacatcgcta actataaata cgagtatatc ttcattatgg attacaatac tgggaaagaa
      481 ttagctcgag taaaagctga tggaatatat agaccagatg taaatcaagc ttataatact
      541 ttaggaaatg ttggctatca tgtatctttt aacatgcgca acttccctaa taagaaagtc
      601 tatgtaatga tgcgggcaac gaatgatcca gaagggaaca ctaaaggcgg agcacaagat
      661 tttcatgata aacgctggta tttaaatatt ccgcaacgat aaaaatagcc cctcgttttt
      721 gaggggaagt acataatgca aatttgtcca taacttattg attatcttgt aaaaatgcgg
      781 taatttaata gttagtgaga agaggtgctt aattatagtt aaaaaagagg aaatagaagc
      841 agcatgtaac caagcaaatg ttaggtttat tggagaagct ttatctggat atcctaagta
      901 taaatactta cacaactggt tacaaagtaa aagtttttat cttaataaag aaagatgcat
      961 gcctaataaa aattataggg tttataaaag aggaactatt gtatatgttg attttggggt
     1021 gaacgtgggt tatgagttat ctggtaatca ttttgctatt gttttaaata ataaagataa
     1081 ttcgaaaaat ggtgttgtat gtgtagtacc aatctcttca aaaaagaaaa gtaattatgt
     1141 atctgtagga aaaatattag aagttgctag tattaaacaa tttataagcc aagctgataa
     1201 attaaaagag aaacttagga taattatttt actttcatta aatagaggca tcattgatga
     1261 aagcgatttg aatagtttta ttaaagatat tgaagctaaa ggtgaaaagc tttcagtgga
     1321 agaagtagaa aaacgagctg taaaacataa tttggtttgc gagacaaggg aacaagtaat
     1381 taaagaaata aaatcattat ctgaagatat ggtaaaatat cagaaggtgt ttaattgcta
     1441 caagaaatat acacaagaat cctatgtaat gccacttaat atccaaacga ttagtaaaaa
     1501 tagaattaga agaattaaca agtttgatcc ttctggaaat atgaaggcac ctggaaatgt
     1561 cctcaatgaa gtagatgaaa gtttaaaaaa atattttaca aagtaaacga tatttttgtt
     1621 gacatttttc tgttcgttag ttaccatgta gttatagaag gacgtaagcg cctattttta
     1681 atgtacacat cgggacgtat gcgcccacat agaaacatag ttgagagaca tctatatggt
     1741 gtctcttttt ttattaatat gcaaaaaagt ctagtttttt caataatatt tatgtaataa
     1801 taaattagcc accacaacaa agaatgaaac ccattattat ctagtctatg tccattcttt
     1861 ttgtttgcag tagttgtgat ggctttccgt actcttagct cagttggtta gagcagacgg
     1921 ctcataaccg tccggtcgta ggctcgagtc ctacagggta catactctaa gtgatttaaa
     1981 ttgcgaataa aatataaaaa tgactcccct tttgactccc ccaagcagta tatggatgtc
     2041 tctaaaagtt gtttttttag ataataaaaa gctgataata agcgaaaaac acttaaaatc
     2101 agctttaaac cctattctcg aaatccggcg aaccggctaa taccggcgcg caggttcaaa
     2161 tcctgtacct tccttatatc aacgattaga ggttgtgaag aagctgtcct gcatcaagta
     2221 ataagaacgg ccaaacaaaa atagcttttc ctattttttc gtttgattgg gcttattacc
     2281 gcagatgcaa gcttttgaac accgtttgtt cggaataagg ggctgtgaca agacttttgt
     2341 cacagcccct tattgttgta cttttaacct acaatatttt ttgattcccc ctgagtacta
     2401 ttttttataa atgctagtaa atcaatcttc tttggccttt ttttgataaa aaaaatacta
     2461 gatgtaagat aaaagtgtaa aaacgatttg ttttaggagg ggaaacgatg tgtaccgcaa
     2521 tcacttatgc aacgaaagac cattattttg gaaggaattt tgattatgag atgtcttata
     2581 atgaagtggt cactattact ccaagaaatt atcgtttcga ttttagaaag gtaaaaaatt
     2641 tagataaaca ttatgcaatg ataggaatag cagctggggt atccaactat ccactctatt
     2701 atgaggctac aaatgaaaaa gggttgagta tggcaggact gaattttcct ggcaatgctg
     2761 attataaaga attacaagaa ggaaaagata acgttgcacc atttgagttt attccatgga
     2821 tactagggca atgctcaacc atagacgaag ctaaagaatt attggctact atcaatctag
     2881 tcaatatcga ttttagtgaa aaactaccct tgtcaccttt acactggtta ttagctgata
     2941 aagaaaaatc gatagtaatt gaaagtatga aagatggact tcatttatat gataatcctg
     3001 tcggtgtgtt gacaaataat ccgccatttg attatcaatt atttaatctg aacaattatc
     3061 gctcgctatc aaatgga
//
LOCUS       DASJST010000214         3048 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJST010000214 DASJST010000000
VERSION     DASJST010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.218"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            541..1446
                     /locus_tag="U4B05_002796"
     CDS             541..1446
                     /locus_tag="U4B05_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2051881.1"
                     /translation="MELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSEQLSSGNLG
                     FRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQITPHFIYN
                     SLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHINDYLVILKIR
                     YGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITTCKIDEMIQ
                     FKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGNDQKPITIDS
                     KYREGTTVTLMIPLSPKTIGGNKHV"
     gene            1439..3004
                     /locus_tag="U4B05_002797"
     CDS             1439..3004
                     /locus_tag="U4B05_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2051882.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
BASE COUNT         1024 a          601 c          530 g          893 t
ORIGIN      
        1 aaaaatttaa gcataaaaca gatttttgcc caagcaaaca aagccatgct tctatttgct
       61 gttatccctt tgctactgag catactgtta tattcgcaac agcttttgat ttatcaacag
      121 acgatcgcaa atatccaaaa agcagatgca attgctttga aggttgatca aaaagttttg
      181 gaaagtatgt gggacttgat ttctggccaa gtaaccgtag atgagttcaa tgaggaaaat
      241 atcctagaag aacttaggaa tgatattcga gagatcaaaa ataatacaaa tacacttaaa
      301 gaaattagta cgttagatgt atcgctacgt acgatcgata cactggaaga ttatcaaaac
      361 gagctgatca ataatatctt ggcaaaagat tttatggata ccaatgaaga gatcatcaat
      421 caggtagatt ctgtcactca actgctgtca gatatcttac aagattttgt tcgtgtggag
      481 atcgacttag caagcaaaaa aaatacgaca cttgtccgct cgctcattct attaacaata
      541 gtggaacttg gagttcttag taccattttt ttctatgcga aaaagaatcg tcgcttttta
      601 gagagaaagg tccaagaacc gatcaatcag ctgatcgaga tgtcagaaca actttcttca
      661 gggaaccttg gctttcgttt aaccctgccg gaaacactgg aattacaaac acttaccgct
      721 agcatgaata aaatggcgga agatctaaac cagtctttac gggagaacgc tttgaaacaa
      781 taccatttag cacaaagtga agttcgtgta ttgcaagctc agattactcc ccattttatt
      841 tataacagtt tggatgccat tatttctttg atcgaacaag actgctttga agaagctctg
      901 actatgaccc atgcactttc cggctttttt cgtacgtctt taagcaaagg acaagactgg
      961 attcctcttg aaaaagaaat tagccatatc aatgattatc tagtcatctt aaaaataaga
     1021 tacggccata tgcttgacta taagatcgat ataccagaaa cactttttcc tgtttctatt
     1081 ttaaaaatga tcttgcagcc attgattgaa aatgctgtat accatggaac gaagtacgtc
     1141 cgtcacaaag gatttattcg tattaccaca tgcaaaattg atgagatgat ccaattcaaa
     1201 gttatagaca acgggattgg catccctgca gaaaaactgg cagaaatacg taaagagtta
     1261 gccaaaggga tcgattcaga tttcagtatc ggctacggac tttacaacgt aaacaagcgt
     1321 ctccttcttt attacggaaa tgatcaaaaa cctatcacga ttgacagcaa ataccgagaa
     1381 ggaacaactg tcacactgat gatccctcta tctcccaaaa caattggagg aaacaaacat
     1441 gtataaagtt tttatcatcg aagacgaata cctcattcgc aataacttgc gggaacaaat
     1501 aactgtcctt ggttcttatt atccaatcac ttatgtcggc gaagctggag atggtgagat
     1561 ggcactgtct tcaattatcg acttacagcc cgacatcatt ctgactgata tccaaatgcc
     1621 tttcatggac gggttgactt tttcaaaaga agcaaggaaa ctacttcctt ggactcgtct
     1681 catatttatt agtggattcg acgatttcga ttatatgaaa ggagcaatcc aggtacaggc
     1741 agatgattat ctgcttaaac cgattaaaag tagcgaattg aaacaagcaa tagaaaaagc
     1801 agtaactgct ttagatttgc agcaaaaaga aacgcacact tcttctgatc ttgttttcga
     1861 attgaaaaaa aatcatttct tgaacggttt gttcaaaggc tcacttacga tcagtgaaac
     1921 attgaaactt gctgaatctt tcaatcgctc tttagcaggg aaaaaaatga ccgtattatt
     1981 agcagcaaac caagtcagta cagactttga agattacgct catctcagtg attatttgaa
     2041 gtttttattt agggagaatc aacaagtgat cttctctgct gtttcctctc gattcatcaa
     2101 atttttaata gttgattctc ttgaagaaaa tgtgatcgag accagttatc aggtagccca
     2161 aacgttggtc catgaattag aaaagggaca aaaaaataat ttttctgtcg ccattggtcc
     2221 tgtcgcagaa agaatcagcg aattacctgc atcttttgag atcacgcaga tgatacttga
     2281 aaccgccagt tcccttaaag aacaaattat cagctatgag gatttgattc aagaagcgaa
     2341 acttccagtt gattctgtct tttactttga cctctcactt gatttgtctt cattatcgaa
     2401 aaacgaagta tctaagtaca tcgaacactt gacgaaacca caaggcacac caactcgaac
     2461 acgattatat cgtttatttg tcttgactaa gctactccag catgtctgcc tgaaagaaaa
     2521 gaaaatcccg caagaaatgg ttcattgcac atccaccagt caacgcttag caatcagcgc
     2581 cgacgactcg ctttaccgtt cgacagttta tatgttcatt tctttcttaa ctgctctgcc
     2641 ctcacagcct tcaaggaaca aatatcagcc aattttgaac catgcattgg attttatcaa
     2701 agaaaatttt accgatccag atatgtcctt aacttgggtc gctcaacaag ttgcattaag
     2761 tccctctcat ttcagtacga ttttttctca atcgtttaac caaacattca tcgattatct
     2821 gacagaacaa agaatcgaat tagcaaaaaa attattgcag caatcagact atcgtatttc
     2881 agatattgct tttgaaattg gttataatga ccctaactat ttcagttatt tatttaagaa
     2941 aaaacaaggg atttctccaa aagaatatcg gacacagctt ttccttaaaa acaaaaataa
     3001 ctaataaacg aaatccgtac aatgatactc tttccatttg caataaaa
//
LOCUS       DASJST010000215         2977 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJST010000215 DASJST010000000
VERSION     DASJST010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.219"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(510..1076)
                     /locus_tag="U4B05_002798"
     CDS             complement(510..1076)
                     /locus_tag="U4B05_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2051883.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="U4B05_002799"
     CDS             complement(1076..2032)
                     /locus_tag="U4B05_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN2051884.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="U4B05_002800"
     CDS             complement(2032..2688)
                     /locus_tag="U4B05_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2051885.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          938 a          583 c          532 g          924 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtagcc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaa
//
LOCUS       DASJST010000216         2971 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJST010000216 DASJST010000000
VERSION     DASJST010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.220"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            20..481
                     /locus_tag="U4B05_002801"
     CDS             20..481
                     /locus_tag="U4B05_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN2051886.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            515..1150
                     /locus_tag="U4B05_002802"
     CDS             515..1150
                     /locus_tag="U4B05_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN2051887.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            1263..1898
                     /locus_tag="U4B05_002803"
     CDS             1263..1898
                     /locus_tag="U4B05_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051888.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            2197..>2971
                     /locus_tag="U4B05_002804"
     CDS             2197..>2971
                     /locus_tag="U4B05_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2051889.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDL"
BASE COUNT         1200 a          425 c          507 g          839 t
ORIGIN      
        1 cagaaatgag gaagaaaaaa tggaaaaaga gattttaata aaatctccag gaaaaacctt
       61 gattaaagta actgatgatt ctatttcaat tattagaaaa ggatttataa atttagttaa
      121 tcaaggtatt aaaggagaaa aaactatccc ctttaaaaat atatcagccg tacaattaaa
      181 aaaaccaggt atgagtaatg gttatataca atttaccttg ctaggcggaa acgaaagtcg
      241 cggaggtata ttagcggcca cgaaagatga aaacaccgtt atgtttacaa aaaaatattg
      301 gaatgaaatg gaaaatttaa aaaaatatat agagaagcaa caatctatat tagataataa
      361 ttccaaagaa acacaaattt caagtgcaga tgaaattaaa aagtataaag agttgttaga
      421 tgaagggatt attaatcaag aagaattcga ttttaagaaa aaagaattgc ttggtttata
      481 atattcaata tcaaaagaga aagaggatct attaatggac ggtatatttg gttttactgg
      541 tattgtttta tttattatag gtttaatcat gttaattgta agatttatca aaaaaacaaa
      601 taagaaaaca cctatgattc tccttattat cgggattatt tttactaccg taggtttctc
      661 gctttcctca tcaaacgagg caaatgacaa taacaaacag gaaagctcat catctgatac
      721 tcaaactact acatctaaca gcagcaataa aagcctaact caatttgaag aatttttaga
      781 gacaaataat catgactggg gtgctttctt agatagttat tattctatta ctccagctac
      841 tgagcaaaat acagctttta ctaaatatat tagtggtaaa acatatactt ttgaaggaac
      901 tgttatcgaa tcaatgacta ctagaatagc cattatagca gacaaagagt acgatgataa
      961 aagttggaat gatatttctg caacacctaa agtttcatat gtaatttttg cgaaggactt
     1021 aaacaatgct gataccttta caaagggcga taaagtaact tttacaggag aaattagttc
     1081 aagaggatcg aacatagaaa aatcctatgc tcaatgggat atgattaata gtaaagtaag
     1141 taaaaaataa atgaaccacg ccccactaag agttagattg tgcacaataa taaaacctat
     1201 acaaaagagt gccatcagga gtcacaatgc ttgtaaagga aatgacaaaa taggaggtaa
     1261 ctatggataa tagcaatgaa aaactaacca aaaaagaata tcttgaatta atagttgaag
     1321 gcggtattgg agcaattcct tatattggtg gcgttgtgca gtcactttat tttgggtcta
     1381 aaaacgagaa gcgtttcaaa cgcatcgaga aattctacaa aaatttaaac gaatcattag
     1441 aaaacattcg cgatcaaatt ccagaagatg ttttcaactt agagaacaag gaccaactca
     1501 ttggaatatt cgaagggata aatgacgagg tagaaaaatc aaaggcacaa aataaaatag
     1561 atatgtataa aaatctttat aagaattgtt tacttcgaat taacaacgct tcatgggata
     1621 acgaggagta ttatcttctt gtactgaatc aattaacatc gattgagatt caattgttag
     1681 catgcttaat gagtcgtgga aacgaaaatt tcactggaaa tatcagcatg tctggctatt
     1741 cacaagagct aatagatggt agcttaaata gattatctga ttttggtctg ttagaaaaaa
     1801 cgattagctc catagtatta ggtgatgtcg gcaaacaaaa tatgggctac aaaatatctc
     1861 atttaggaat tcaatttatt aactcaactc ttacttaagt tttctttcta atatgacatc
     1921 tagacctatc agcaatgcaa caataaaaaa ccagacatac caataatcaa caaataatct
     1981 aaactctgga agttcagtta ttcttttaaa aatcacttta atcataataa acacctctga
     2041 ttggatgatc taatacacat ccaattatac cattaaaacg acaaatagca catccttgcg
     2101 ccggcaagca aaaaggatgt gcaaaaaata ataaaaccca aacaatgggc ttctttatag
     2161 ttcctattgt atcagagaaa gagagctgat tcaattatgg caagattagt caaacgtgga
     2221 aatagttggc aatacgaaat ttcatacaaa aaagatgacg gaaaatacac gaagataaga
     2281 aaatcaggat ttaagacaaa aggagaagca aaagatgccg ccaacgaatt agaatataac
     2341 ttgaacaaag gccttaaagg ggatcgcaaa aatctattat tatcagatta ctttgaggat
     2401 tggatgcaac tttataaaga aggaacagta tctcctatca cttatagaaa atacgaagat
     2461 acgttaatga acataaagaa atatatgcca gcggtattga tttctgattt agatagagtg
     2521 ggatatcaac gctttttaaa taagtatgct aaagaccatg taaaatctac cgttattaag
     2581 tttaataacc atattagagc gtcgttgaaa gatgccgtag aagaaggatt aattccgttt
     2641 gatccaacta gaaaagcagt aatcaaagga aaagattcat tgaagccaaa agaagataaa
     2701 tatttagatt atgatcaatt taaatcttta atgaaactcg tagaagaaaa cctttctgcg
     2761 cagtactctt ctcctatgct cgtgttagtt gctggtgcca ctggaatgcg atttgctgaa
     2821 cttctaggat taacatggga agatatcgat ttcgaagatc aaatcatcac tattaataaa
     2881 acatggaatt ataaattaaa tgaatgggga aaaacaaaaa acgaaacttc aaataggaaa
     2941 atttccattg ataaacatac gattgatctc t
//
LOCUS       DASJST010000217         2969 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJST010000217 DASJST010000000
VERSION     DASJST010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.221"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..832)
                     /locus_tag="U4B05_002805"
     CDS             complement(<1..832)
                     /locus_tag="U4B05_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051890.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     V"
     gene            complement(1067..1342)
                     /locus_tag="U4B05_002806"
     CDS             complement(1067..1342)
                     /locus_tag="U4B05_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN2051891.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(1585..2895)
                     /gene="der"
                     /locus_tag="U4B05_002807"
     CDS             complement(1585..2895)
                     /gene="der"
                     /locus_tag="U4B05_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN2051892.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT          874 a          625 c          509 g          961 t
ORIGIN      
        1 acacaaaaaa gttttttagt tggaaatcaa aaggatatgc ttggagattc cacacgaaat
       61 gaaacatcat tcctaaaaat tttttcatac tagcagaaag tagatggcca ttttcggtca
      121 gataaaaagc ttgttcaaac agtttaccat aatttgacag gttttctgcc tctaattgat
      181 aaaaatagat catttcttcg atcatcccgg caaacggccg agtcgttaat tgctttttat
      241 ttggatagtt tttatacaga ttttgcaaaa aacgatgatc acaaatcatt ccgcaacaga
      301 taaaagtcaa aaagtcaatc tgctttccgt ccacctgccg ctcgctgatt gtttgatcat
      361 aaacgatgcg ttctggcaga ttgatggttt gcagcactac acgatttggt agctgcaaaa
      421 tcgtccgctg cggtgttaat gctcggctta gagaacggat cgatacaggg atcatccata
      481 gtgatgaatc aagggttgta tgtttttgaa aaaaacctgc cagatccatg atcccttcat
      541 ttccaaatcc gatgataaac atggattgat tttcgggata gcgtttgacg aaatcaagca
      601 ggttctttaa ttctgttaaa tgattgcatt gagtgttggc acaaatatac cagtcattgt
      661 ttgcttgatt tgcaaataac gggttgatct tatctgcaaa caaatcataa tagcgctgat
      721 tcgttaaaaa taagattggc tgatcaatgg aaagttctct tagctgactg actagcgttt
      781 ctccgtaaaa cactttcgtc aacgcatctt ctacttgata atcaagttcc atacgcatcc
      841 ctcctcttct tatttttatt ttaccattag taagcaaatt ctagaaaaga taagagcaaa
      901 gctcacctca taacttgcaa ataaataaaa attaaataaa atacatgtgt tttctatgta
      961 tgacatacgg attcggaatc tctgtctctt atttattcct tttctgctag gaaataaaaa
     1021 gaaaagcact tgtagcccta gagccacaag cgctttttca actgtcttat ttaacagcgt
     1081 cttttaatgc tttacctggt ttgaatgcag gtactttgct agctggaatt tcgatttctt
     1141 caccagtttg tgggttacga cctttgcgag ctgcgcgttc gcgaacttca aagttaccaa
     1201 aaccgattaa ttgaactttt tcgccgttag ctaaagcatc ttgaatagtt gaaaatacag
     1261 catctactgc tgcagttgca tcttttttag ttaaatcagt agctgaagca actttttcga
     1321 ttaattctgc tttgtttgcc atggatagat tcacctcctc cacaagagat atgatagaaa
     1381 tatcatttag ttaaaacttt ctgagtggtc cagaagttta aaaagaaata ttgattaact
     1441 tcaatattct gttatcacga tatcatatga accgcttaat agcaaggttt ttaccaattt
     1501 tcacatgctt tttttagtca tagaaacgta acaaacgaaa ctgtcttttt cttttgaaaa
     1561 aaaaacgtcg atatggtaaa atgcctattt tctcctacga gggataattc ggatggacgt
     1621 cccttcaaaa gtaaatgctt ttctgatttg attttctaag aaacgttcat aagaaaaatg
     1681 catcaattcc tcttcattga caaaaacgac aaatgtaggt ggtttgacag cgacttgtgt
     1741 agcataaaaa atcttcaggc gttttccttt atctgttggt gttggattga ttgctacagc
     1801 atccataatg atatcattca acacagccga agagatacga agattctggt tcatacttac
     1861 ttgttcgatc atttcaggca gacgttccaa ccgttgttta gtcactgcag aaacaaaaac
     1921 gattggagcg taatcaagat accggaattc atcacgaatc tcttgttcga aatcacgcat
     1981 tgtattggtt tctttcttta caagatccca tttattcacg acgataataa ttccacgacc
     2041 agcttcatgg gcgtaacccg ctactcgctt gtcttgttcc cgaatccctt cttcagcatt
     2101 cagtaccatc aaaacgatat cagaacggtc aatggcacgc attgcacgca tcacgctgta
     2161 tttttcagta ttttcgtata cttttccgcg tttacgcata ccagctgtat ctatcattaa
     2221 gaatttctga ccactttcag aaacgaagtg ggtgtcgatt gcgtctcggg tcgttccttc
     2281 aatatccgaa acaattacac gttcttctcc taaaatcgca ttgatcaaag aggatttacc
     2341 aacatttggg cgaccaatca aactaaactt aatcgtgtcg tcgtcctctt cttcagtctc
     2401 ttcagagaaa tacttcactg cttcatctaa aatatcacca atccctagtc catggcttcc
     2461 tgaaacagga taaggatcgc ctagccctaa ggaataaaat tcatagatat cgtttctcat
     2521 ttctggatta tctactttat tgactgctaa aatcactggt ttattacttc tgtataaaat
     2581 tttcgcaacc atctcatctg cgtctgtcac gccttcgcgt ccgctgacta cacaaatgat
     2641 tacatctgct tcttcaatcg cgatttctgc ttgatgcttg atctgatcca taaaaggttc
     2701 atcgccaaga tcaattccgc ctgtgtcgat cacgctgaat tctcgaccta accattcacc
     2761 ttttgcataa atacgatcac gagttactcc aggagtatct tcgacaatcg agatacgctc
     2821 accagcgatg cggttaaaaa tcgtagactt accgacgttc gggcggccaa cgattgcaat
     2881 tgttggattt gccatattat ttcctcctca caagtttcca atctcactta gtttaacgaa
     2941 tgcattgcca actttcaagc atagattgt
//
LOCUS       DASJST010000218         2949 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJST010000218 DASJST010000000
VERSION     DASJST010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.222"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..1048
                     /locus_tag="U4B05_002808"
     CDS             41..1048
                     /locus_tag="U4B05_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2051893.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDAIELMSGQEFIRIAKENNVVT
                     W"
     gene            1060..2064
                     /gene="neuB"
                     /locus_tag="U4B05_002809"
     CDS             1060..2064
                     /gene="neuB"
                     /locus_tag="U4B05_002809"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEN2051894.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2061..>2949
                     /gene="neuC"
                     /locus_tag="U4B05_002810"
     CDS             2061..>2949
                     /gene="neuC"
                     /locus_tag="U4B05_002810"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN2051895.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAAS"
BASE COUNT         1094 a          379 c          572 g          904 t
ORIGIN      
        1 tcgaattata gatgactata attcatagga ggagatataa gtgtttaaag gaaaaacaat
       61 attagtaact ggcggaactg gttccattgg aagtgaaatt gttcgtcagc ttttaaaata
      121 tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa aaacaatttt atatgcaaca
      181 agagctctca aatcacacta atttgagatt tttaattggt gatatcagag actctcaaag
      241 gattgattat gcatgtcgtg atgtggatat tatttttcat gctgccgcaa tgaaacacgt
      301 tccagcaagt gaatataatc ctatggaagc tgtaaaaaca aatgtgatgg gaactcaaaa
      361 tgtaatagat gcagcgatca aaaataatgt aaaacatttt gtaggtatca gtacagataa
      421 agtagtgagt cctactaaca ccatgggtgc gacaaagtta ttgagtgaaa aattaataat
      481 agctgccaat cagtataaag gaaaaacaga aacaatattt tcttgtgtaa ggtttggaaa
      541 tgtcatgggg tcaagtggat ctgttattcc tctttttagt aaacaaataa aaaacaataa
      601 agatattact ttgacagatg ttgatatgac acgatttatg atgtcaatac cggatgctgc
      661 aagattagtt ctgtctgctg ctatgaattc aaaatttgga gaaacatatg tcttaaaaat
      721 gccggcaatc aatattttaa ctttagctca atcattaata aaaatatata atgatacaca
      781 tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc aggccaggag aaaaattata
      841 tgaagagcta atgactacag acgaaattga acgctcatat gaaaatgata aaatgtatat
      901 cattccacca cactatcatg ctactgacga actgtacaaa gattatgaaa aaattaaaat
      961 cactgcatac tcttcagatg caatagaatt aatgagtgga caggaattta ttagaatcgc
     1021 aaaagagaat aatgtcgtga cttggtaagg agttttatga tgaaatcaga agtatatgta
     1081 attgcagaaa ttggtgtaaa ccataatgga agtctagatt tagctttaga aagtattgat
     1141 aaagctaaag aatgtggagc taacgcggtt aaatttcaaa catttaaaac gaataagcta
     1201 gtttccaaaa aggctccaat ggcaaaatat cagaaggata atcttaaaga ggacagttct
     1261 caatttgaaa tgctaaaaaa attagaatta tctcaatctg attttattag aataaaagat
     1321 tattgtatac aaaataaaat tgattttttg tctactcctt ttgatgaaga aagtgcagac
     1381 tttctaaaac aaattggtgt acaaggattt aaaattggct caggagattt aacaaattta
     1441 ccttttctta gaaaaataga tatgtatggg ttgccagttt tattatctac aggtatggct
     1501 gatattcatg aggtcagtga ggctgtagat tgtttcatag atagtccagt tactatttta
     1561 cattgtactt caaattatcc ggctagttct gaagatatta atttattggc gattaacaca
     1621 ttaaaagaaa tgttcgggat acccgttggg tactccgatc attcattggg atatgatgtt
     1681 ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga aacattttac cttggataag
     1741 gaactacctg gtccagatca taaagcttct ttagatcctc aggaatttgc agattttgta
     1801 aagcatatta gaaacactga aatattttta ggtgatggtg ttaagcgagc aatgccttct
     1861 gaacttagta caaaagaagt atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg
     1921 ggagatattc ttacagagaa atctcttaca ataaaacgtc ctggaaatgg aatagctcct
     1981 aagtttttat atcaatatat tggcaaaaaa gttaaaagac caataagtgc agatagtgtt
     2041 ctgcttgagg aagatgttga atgaaaaaaa tacttatagt aagtggaaca cgagctgatt
     2101 atggaatata taaacctgtt gctgagaatt tactacaaga taattgggat gtatcatttg
     2161 ttgtttctgg aatgcattta tcccataaat atggatttac taaagaattg atcttagctg
     2221 acggatttag aattagtgga gaagttcaaa gtttgttttt agagaacaat aagggaaaca
     2281 tggctcggtc tgtggcatta gagataaatg gtttcacaaa tatatttgaa caaactagcc
     2341 ctgattttgt attattacta ggggatagag gagaaatgtt ggcagctgcg atagcttgtt
     2401 tgtatcttgg aatcaaaaca gcacatattc acggaggcga agtgtctggt actgtagatg
     2461 aatcaataag acatgccata acgaaaatag catctgttca ttttgctgct acggaaaaaa
     2521 gcaaagagcg tttaataaaa atgggggaag attcttggag agtttttccc gtcggagctc
     2581 ctagaataga tactatttta aataatagat tacctagttt tagagatata aaagaaaaat
     2641 atcatctaaa atttgaaaag aaaaaatacg ttttgcaagt atttcatcca gttgtaaccg
     2701 aactagaaaa tatagaaaaa caaattttag ctttgatcaa ttcaatggaa caacaatctc
     2761 aaccaattat ttgcatttta cctaatagtg atgctggtag cgaaattatc agaaaaatgt
     2821 accagaaata tttaaaaacg gatattattt atattacgaa tttagagccg gaggaatacc
     2881 tgactatatt aaaagagtgc aaatttatga tcggaaattc ttcttctggg ataattgaag
     2941 ctgcatcat
//
LOCUS       DASJST010000219         2928 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJST010000219 DASJST010000000
VERSION     DASJST010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.223"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..119)
                     /locus_tag="U4B05_002811"
     CDS             complement(<1..119)
                     /locus_tag="U4B05_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2051896.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYK"
     gene            complement(272..598)
                     /locus_tag="U4B05_002812"
     CDS             complement(272..598)
                     /locus_tag="U4B05_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051897.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(610..810)
                     /locus_tag="U4B05_002813"
     CDS             complement(610..810)
                     /locus_tag="U4B05_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051898.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            933..1535
                     /locus_tag="U4B05_002814"
     CDS             933..1535
                     /locus_tag="U4B05_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051899.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1590..2756
                     /locus_tag="U4B05_002815"
     CDS             1590..2756
                     /locus_tag="U4B05_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2051900.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT         1032 a          494 c          424 g          978 t
ORIGIN      
        1 ttcttatatt tttcatttac gtttaaaaaa ataggatatt gatagtataa ttctgcatat
       61 atatcagaat tagaatagcc ttgcttttca actaagcttt caacttcttt ttcattcatt
      121 gtcacacctg cttctgattt tatttgctat aatagattca aataaagttt tctcagaact
      181 ttattattga gtgttccccc gatctgcaaa ctgacgggaa cactcttttt aattttttct
      241 acttcatcaa atttaaaaaa cgaggtgttt tttaaatttg atgactcaat atccattttt
      301 tgcattcttc ttttgaaaac aactccattt tttctaataa aataactggc aatcccattc
      361 ttttaaattt caaaaaagtt gtatatgata cattcaaata tttagccatc tcttttcctt
      421 ttagaaatgg tctttctaat ccggtatcaa ttcttgcttg atcaatagcc tcttttgtca
      481 tttcaaaaac ataatttttc aattgattcg tttgttgttt atctaacatt actgtaattc
      541 ctgcatccat tatttttctc ctctcatttt ctatttcttc aatcagccaa ctcgccaagt
      601 tgtatttatt tagattttca aatcattaag taaccaattt accaactttt gataagtaga
      661 tgctctaata ctttctttgc tcccattttc gattttgaca tatgttttcc gatttatacc
      721 tatcaatttt gctgcatcgt ttttagtaag tataagatcg gctctatgtc ttcttaagct
      781 tctaatgaat tgatcattaa gagttatcat tcagcgcctc ctttcaaatt attcactttg
      841 tggtttttat cttaataagc acattttaaa ccacaaaacg agtaatatca attaaaatat
      901 tactcaattt gtggttttca tgctataata gtttggaatt tactataggg ggcgttactt
      961 tgaatagaat aaaattttat attgattcca gtaaaaagac cattaaacaa attagtacag
     1021 aaactggtat taactataca actcttacga actattaccg tggtgagcga aatcctagaa
     1081 atactgaaac atggaatatc cttgctgatt atttccatgt tcctgtaagt tatttgatgg
     1141 gattagacga aacaaattcc atagaacaac ctaatcttga tagaataaaa actgctcgtt
     1201 tagaaaaaaa ggacaaaata gtcaagaata ttgccagtct tgactcagat tattttactg
     1261 aattatctaa tctatacttt tccatgactt ctttacttga agtcaaactt caagaagatg
     1321 actccgactt tcttttttta aaaagtattc tagaggactc ctttgaatac acgattctat
     1381 ttgccaaaca aaagaagttt attaataaag ctttaaactt aaatatcgat gaaagaaaag
     1441 agttagaatc tgagttattt ggcaacttaa cccaagtaca ggttaacaat ctaaaaagag
     1501 agataacagc tcgattaata tcaaaacttc aataaaacta attattcaat cagccaactc
     1561 gccaagttat cagattggag agattagtta tgtcaataca aaagtacaca acaaaaagcg
     1621 gtgcacgata tagtgtccgc ttgtatgttc gtaaagatga aaatggaaag caagaatact
     1681 atgtaaaaaa agggtttaaa actgaaaaag aagccaagtt acacgaagca agaaaaaagg
     1741 tcgagattga agataccggc tatcataagc cttctaatga cttgtttata acagtgtatg
     1801 aagaatggtt gccttcttat aggaatcgtg tacaagaaac gacatatcaa cgtacaaaag
     1861 accttttccg cttgcatata ctaccaaaat tcgggaataa gtccatttct aagattactc
     1921 ctgttcactg tcagaatgca gtcaatgaat gggctttaaa attcaagaat atcaaacagc
     1981 ttaagtctta cagctctcaa atatttgagt atgccatttt tgcagagcta ttacaccgaa
     2041 atcctatggc aaatacaaat ctaccaaagc gggaaaagaa agaaaatgaa aattactttt
     2101 cattagaaga actaaaagct ttttgggata ttctcaataa agaagagcct ttaaaacata
     2161 ttttaatctt ccagcttttg attactactg gtatacgtaa aggagagcta gctgctcttc
     2221 attggtctga catttatttt gaagaacagc taatgtatgt tagaaagagc tatgcaacta
     2281 ttcgaaatga agagaagaat gcaagaagaa aaactgtcag gattcaaaaa aatacaaaaa
     2341 atatatcttc cgaacgaata ttaccaattg atgcacaaac tattgaaatg ttaaaaatct
     2401 ggaaaaaaga acaagctcga gagcttttgc agtttggcat aaatacaaat acaaaaaatc
     2461 aacttatctt tacctatgta aatgcagacg gtgacattaa tcaacctttg catgcggact
     2521 attcaaataa tatattgaaa cgtttagaga agaaatataa atttaagcac gttactattc
     2581 atggattccg gcacactcac gctactcttc tactagaagg tggagcaagt atcaaagaaa
     2641 cacaagatcg attgggtcat aaaaatgctg aaactaccct taacacttat tctcatgtta
     2701 ctgaaaaagc tcaacgaaat gcgttagaaa aattcattgc ttacactggt ttataatatt
     2761 gtcattacct ttctcattac cagaacgaaa aaaagacacc ttctcgcaat gagaaagtgt
     2821 ctgaaatcgt tggtataaca acgattaacg ttttgaaaac tgtgaagctt tacgtgcttt
     2881 cttaagacct ggttttttct tcgtatcatt gaataagttt gaaaagcc
//
LOCUS       DASJST010000220         2913 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJST010000220 DASJST010000000
VERSION     DASJST010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.224"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..217
                     /locus_tag="U4B05_002816"
     CDS             <1..217
                     /locus_tag="U4B05_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN2051901.1"
                     /translation="EQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNS
                     SRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            190..867
                     /locus_tag="U4B05_002817"
     CDS             190..867
                     /locus_tag="U4B05_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2051902.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            857..1999
                     /locus_tag="U4B05_002818"
     CDS             857..1999
                     /locus_tag="U4B05_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN2051903.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2012..>2913
                     /locus_tag="U4B05_002819"
     CDS             2012..>2913
                     /locus_tag="U4B05_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2051904.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLE"
BASE COUNT         1075 a          367 c          566 g          905 t
ORIGIN      
        1 agaacaatct aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg aaactgcttt
       61 gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga acatctacgg
      121 tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga atgatgggga
      181 agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga tccatacatt
      241 attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt agaaaataag
      301 ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa atatcttggg
      361 aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa atttattgta
      421 gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat gccaagtgag
      481 caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc aattggttat
      541 ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg agagcatgct
      601 ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta tgttcattta
      661 tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca aataggaatt
      721 ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg cgcagggagc
      781 actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc taaaattaag
      841 aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata tgggaggaac
      901 agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat taggtgaaaa
      961 tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg gagcttctgc
     1021 gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg ttggaaaaaa
     1081 cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta ttttatatca
     1141 aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga gccctgttgc
     1201 actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg caatcatcgt
     1261 agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg caaaccaatt
     1321 taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata agggaatttc
     1381 gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga tcatcacaac
     1441 ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt caagattttt
     1501 agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag gttataatta
     1561 ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat tgaatgatag
     1621 agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg gaattgatag
     1681 aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt ctactttaac
     1741 gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac taaatgaaaa
     1801 aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat ttcaaggggt
     1861 aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta atagcggctt
     1921 atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta ttgatgagtt
     1981 taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg gtttaggttc
     2041 catggggaaa agaagactta gactcattca agaacacttt aatgatattt tactatgtgg
     2101 tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa taccaactta
     2161 taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg tttgtacatc
     2221 ccctataacc catgaattta ttataaaaga ggctttaaat ttagattgta atatctttac
     2281 ggaaatcaat ttattaaatg aatattatga tgaaataatc gaagtagcta aaaagaaaga
     2341 tagattactt tatctatctt ctacattttt gaagagaaaa gaaattcaat atattaagtc
     2401 taaagtgacg cataataaaa aaatttctta tcaataccat gtaggacagt atttacccga
     2461 ctggcatcct tgggaagatt atcgcaattt ttttgtttca aacaaaaaaa ctaatgggtg
     2521 tagagaaata ttcgctattg aattaccttg gattatagat acatttggga aagttaaatc
     2581 gtttactgta atgacccaga aattatccaa tcttgatatt gattattcgg acagtttcac
     2641 tcttattatt gagcatgaaa ctggtatagt tggaacttta agtgtagatg ttgtttcaag
     2701 agtagctaaa agaaatttaa ctataattgg tgaaaagatt ttaatagaat gggaaggtaa
     2761 cccggattct ctattttcat ggaatgaaga aacttctagt atgacagata ttcaattgta
     2821 cgatgaggtt atgcatgata ataattattc acatactatt attgaagatg catatttaga
     2881 ggagattaaa gaatttttca cgcttttgga aaa
//
LOCUS       DASJST010000221         2883 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJST010000221 DASJST010000000
VERSION     DASJST010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.225"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(19..1362)
                     /locus_tag="U4B05_002820"
     CDS             complement(19..1362)
                     /locus_tag="U4B05_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2051905.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(1496..>2883)
                     /locus_tag="U4B05_002821"
     CDS             complement(1496..>2883)
                     /locus_tag="U4B05_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2051906.1"
                     /translation="GEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEA
                     TQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDT
                     KYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVE
                     VGGTVDLTDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKV
                     PVEVTDNRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT          798 a          562 c          386 g         1137 t
ORIGIN      
        1 gatttcattt agtaacgatt atgtttcaat gtttaatcct gttactgcct ttaaaataaa
       61 attatagggt tggctaaaac taggatgaaa taaataatct aacattttca atttgtctaa
      121 tgtaatttgt tcttctatag caagagaaaa cattgataat agtccagata aatcttcttg
      181 actacaaagt tgtacaccta ctattctctt tgtatttttt tcaaagctaa ttcttaattt
      241 tacacgacta ttttttctca aaaaaacagg tctaatgaaa tcttctattt ctatataatc
      301 tatagactca cttaataatc gtgctgtttt taaattcaaa cctgctgcgt aaaaattgta
      361 attaaatata gaaaagccat ttgtaaattg ggaaccagaa ggtgtaaaat cttgattgtc
      421 aatatgttga gttgcaatat aagctgttct tagagcatta gaaatagaaa aatcaacact
      481 acttctatta atggacgtaa aaaaagttgt agcacaatcc ccaatagcat atatatcttt
      541 tatatttgtt tgctggtatt cattagttag atatgctcca ctaacatctc tcaagagttc
      601 ctttttgcca attggactat tagatagaaa tcctatagct tgtacgacca tatccataga
      661 gtattgggta ccacttgtag tgactatttt ttcaatcttt tttcttccta aatatttttt
      721 aggactttct gatagacaaa gatgtattcc attttttgat agtatttctt caactttatc
      781 tgaaaaacta cggtcgtaat aagtcgacaa cactctatct gcaacatcga aaagatatac
      841 ttctttccct ctttttctta tggcttcagc aatttcaact ccaatatatc ctgctccaat
      901 aattcctact tttttaatag tattatcatc taaagcttta aaaatctctt tagcatttgg
      961 aaaattcttt atcgaaaaaa tattagctaa attattcccc ggtacatcta acataatttg
     1021 ctttgaacct gtagctagta taagtttgtc atattcttgg gaaaatcttg tgccatcttt
     1081 ttttacacaa ataatctttt tattttgaat atcaattttg tcaactgctg tctccaaaaa
     1141 caactttttt atcttagaat ttagttctct tctattttga taaaaaagat cttctaaatt
     1201 gctaacttct ctttttatga acaacgatgt cccacattct aagtaactaa tttctgattt
     1261 tttatctatc agaataactt cataatttga atcacataag tttagaaatc gagctgcagc
     1321 tagccccgaa tggtttgatc caataattac tatttttttc acttaatcac attcttttcc
     1381 cttctatgaa aagtatcttc atttttaact aacaacctat ctcccaaaat aaaaaacaaa
     1441 aagaggaaga aaaagattct tcctcttttc agagaagatt taagaaattg attttctatt
     1501 cattctcttt ttttcgattt ttatacccta ataaccctaa agttcctaac aggattaaac
     1561 caccactaat agtaaagata ttactatcaa tctctcccgt cttaggataa gcttttgagg
     1621 actgcttttg aggactgctt ttgcctctat ttttgttttg cttgctacct tgattgtcag
     1681 tcttattatc tttactaacg gctttttcac tgtctttttc gtttgaagaa acaactacat
     1741 tagtaggttc actagtatta ccagattcat cagttgcagt tactatcact tttgttcctg
     1801 cctctaattt aggaacttta actgtaaaat taccattttt atcagctgtc ccatggtatt
     1861 gactcccgtt tggtaatgta actgtcacct ctgcatttgg ttcagtttta ccagtaacaa
     1921 tttggtcacc ctctgtaata ggatcaactt gaggtttatt cggtgctttt ttatcttcta
     1981 cagaaatagt aacctctact tcttctttag atccatctgg atatgttact actacaatcc
     2041 cttttttgtt tccagctgta ctaacatcta ctttctcttt ccaagtataa gtagttcctt
     2101 ttggtagatc ttctttgttc ttaattccct cagatgcgtc tggctcttta tttaagtctg
     2161 tagtcacttt ttgtccttca ggattgtatt tatcggcatc ggaacgattg tctgtcactt
     2221 ctactggaac tttcactgta tctttcgtac catctggata cgttacttcg ataacccctt
     2281 cgtaattacc tggtgtgttg gtatcaatcg taccatcagg agtgacatct gttactgttg
     2341 ttccttctgg taatgttggc aagttagtaa cattatctgt tagatctact gtgcctccaa
     2401 cttctacttt ttcgccttct actgttggct catatttatc ggcgtcagaa cgattgtctg
     2461 tcactgtaac atctacttca acttcatcag atgatccatc tggatatgtc actacaacag
     2521 tccctttttt atttccagct gcgctaacat ctactttttc tttccaagta tacttagtat
     2581 cttttggtaa atcttctttg ttcttaattc cctcagatgc gtctggttcc ttattcaatt
     2641 ctgtagtcac tttttgacct tcaggattat atttgtcagc gtcagattta tcatccagtt
     2701 ttgttgcact tgatacaaat tctgtatcat ctgctgtaat tggttgagtt gcttcttctg
     2761 gccatttcgc atctgtataa ccatcttttg ctttcaacac tggaagttta tcttctggta
     2821 agcttacgcc gtctttcact gcaaagaccg ttgttccact ttcaatactt gttccttccc
     2881 ctg
//
LOCUS       DASJST010000222         2880 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.226, whole genome shotgun sequence.
ACCESSION   DASJST010000222 DASJST010000000
VERSION     DASJST010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.226"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..236)
                     /locus_tag="U4B05_002822"
     CDS             complement(<1..236)
                     /locus_tag="U4B05_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN2051907.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            complement(591..1193)
                     /locus_tag="U4B05_002823"
     CDS             complement(591..1193)
                     /locus_tag="U4B05_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051908.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2265..2477
                     /locus_tag="U4B05_002824"
     CDS             2265..2477
                     /locus_tag="U4B05_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051909.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            2467..2862
                     /locus_tag="U4B05_002825"
     CDS             2467..2862
                     /locus_tag="U4B05_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051910.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          977 a          471 c          396 g         1036 t
ORIGIN      
        1 catttttcaa ctaattcctt gaaagctgca tcatactcag ataaagcttg gttaacatac
       61 atatcaattt caagatctat cttgctttcc tcttttacaa tagcttccat cattttatct
      121 tcatcaataa aaatagtatc tccaggtttc aaaccttttt gcaataaact ctcttttgac
      181 aaagttacaa ctattgaatt acctacttct ctgattttgc gttttttaat attcatatca
      241 tcaactccta cgataagtat acaccgtata tccgtattgt tcaatcaccc ttattacttc
      301 cttcaattac taaactttta aagagttgga aattaatagt tataatgtag ctcttggatg
      361 aattttaact tttcatgatt tattggagtc atatgctatt tttactccca aataattaat
      421 tacggctgaa catgttgtta acaccaaaac tgcatcaggt aaatctgaga caattgtttt
      481 ttcattttct gcatgtcgta ttccattatt actcgccaaa ccatacattg tctcaaatgg
      541 ttttaaaaat gtaagatcga cattgttatt cgatttgtct ttaattgctt ttatagcagt
      601 tcccaaagta tttttttatt agctggtaat cctgaaaatt cgataagaca actttcgaca
      661 gcactaattg cttctctcac tacagtatta taatcttgat tttccttctc gcttatatat
      721 gtaagagatt tatggatatg attttttgcg taattaaatg gtgattcaga tacagcattt
      781 atagacttaa tagaaatatc atctgtgatt ggaataaatt cattgctttc gcttaatctg
      841 taaccagaat tttccgactc cagaatttta tttacttcct ctcttttctt tttatccaag
      901 attttagcac ttgcaaaaac ctctaataaa tcataataag cataccactc caagtcttct
      961 aacctcttga taatatcttc ataataaaca gtaaaatgat aacccaactc atagctatta
     1021 tttatctgtt ctttaaaaaa ctctttaatt aaaaaagaat atattaccca tgagactttt
     1081 ccattataat tactataaac tttatcagcc tctcgtaata tatcataaat cacatttgct
     1141 aaactatttt taagagggtc tgtcaatcta tctctcagta tgatatcctc cattttttaa
     1201 aaccaaatct ttcagaaaaa agcattgcac tacctccagt aaattaactt atactcatat
     1261 aactttacga agcaataatt attcgtgttg ttttacataa ataaaatgaa atatctgtta
     1321 agattgttta atggcacaaa cataaattat tatataatta cgtattaatt tatctagatc
     1381 tattaataat actgatccct aataggtgta aaacagttat aagaaatgag tactctagta
     1441 tttgtgttac ccgaataatt acttgattat attggtttcc catataattg aaggctgtcc
     1501 taatttttac ctactatatg atcataattg attaactctt atatatttcc gaaaattatc
     1561 ttgaagattt ctattggtat ggagaatatc catttcattg ccaataagga tataaattct
     1621 caaaataaca atcgatgtct gtattaaaca agcctagaat ggtaaatctg attcgccaaa
     1681 accattggat tgtatcgatt tgctttacgc gaacgccacc tacccatttt ataaaaataa
     1741 aatgggcaaa agatctttct ttttgtcata caaaaagaag aaaaaaaaga gacgattttc
     1801 ttcttccaaa atcgtccctc ttttttctaa aaccatggat gaacgaacaa aaaatctctc
     1861 gtattttgtt tgttcatcca ctaaaaatat gcgaacagta tggtttcatt caatcctgtt
     1921 cttacaagtc aaaagcatcg aatcgtcctt ggacgattca acaaaaaaag tagtcgtttt
     1981 tggatgacta ctaagttcta aacattcatt acccaaaatc aatttggtct aacatcgttt
     2041 ctcgactggc ttgatctaat cggctcttga cagggctttt tcttttctgt tgatagcaga
     2101 taagaggaaa taagaataag tgtgttcatg tgtcataaaa acagtaacta aaacattaaa
     2161 atcaaaattt atatggaaag cttccttgac attttgtgct cgcaggagta ttataaaagc
     2221 acgaaaggaa agtaaacttt ccatttttta gaaaggagca tgttgtgaaa aatcgtttgg
     2281 aagaattacg aaaacaaaga ggaattaaac aagaagattt agcttctgct cttgaagtat
     2341 ctcgtcaaac aataggttct ttagaaaatg gacgttataa tccctctatt atattggcat
     2401 ttaaaattgc aaggtacttt caaatgagca ttgaagaaat ttttatctat gaggaggaaa
     2461 gtgaagatga aaaataaggg tcagatttgg tatatcggtt atattgtttc gttgtttttg
     2521 attgtgacaa ttttggttac agattttcct aaaatggtag atattggact tgctatttta
     2581 ttcagtgccg tttttggcgt ttctcatgtt caaattttgc atgataaaat gattaaaaat
     2641 gatgcagact acaaaatcaa tgttatggac gaacggaata tcataatcaa agaaaaagcg
     2701 ggaaatataa caaatatgtt taacacaata ctgttagggg tggcaacagt tatatttatt
     2761 tgtttagact acatcattcc agctattatt gtcggaagta tcattgcaat ccagccgatt
     2821 attttaatca ttatcagtag tgtgcttgag aaaaaaatgt agaaataaag gttcatacaa
//
LOCUS       DASJST010000223         2831 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJST010000223 DASJST010000000
VERSION     DASJST010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.227"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(144..1538)
                     /locus_tag="U4B05_002826"
     CDS             complement(144..1538)
                     /locus_tag="U4B05_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2051911.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            1630..2601
                     /locus_tag="U4B05_002827"
     CDS             1630..2601
                     /locus_tag="U4B05_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN2051912.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            2598..>2831
                     /locus_tag="U4B05_002828"
     CDS             2598..>2831
                     /locus_tag="U4B05_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN2051913.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLAT"
BASE COUNT          910 a          448 c          556 g          917 t
ORIGIN      
        1 aataattttt cttgcttctt ggatacttta agtgcaaaat gttcgtttat tttttgaggc
       61 actacccgtt gttgaacctc gggtagtgcc tcttttttat tgaaaggaat caataagatt
      121 tgattccttt atttatcaat aggttataat tcatattttt ttattttttc ttttacgata
      181 cttagtccag aagctaaaat ttcaaggtta ttagaggata gggaaattcg aacgaatgca
      241 tctggttgcg tacgtgtagt aaagcgtctg gaatgataaa gtcggatacc gttagcaaga
      301 aagtcattct caattatctt agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa
      361 gggacacgat tcgttggttt ttctactttg aaaagagaat caaaaagctg attggctctt
      421 tttgtaagac ttaatttagt ttctaagatc ctatttgctt catttgaatg gaggatttga
      481 gtgatgactt ccacatctaa tccagaagtt ttcacattta tattaaaaat tgcttgttct
      541 atcttgcttt ggacatgtac tggaactaca agataagcaa ctcgcaatcc agaacaaata
      601 aattttgtca ttccagctaa gtagattgtt tggttaggta ataaattttg gaaaggagga
      661 atgatcgtat gttgatagta agttgttaaa aatgaatgga tatcatcttc aataacccat
      721 agaccttctt tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac
      781 cctataggat tattacaaga aggcataaga aagacaccat gaatcttctt ttttctacat
      841 tcctgaatca atatatcagg tcgcattcct tgctcgtcaa aatcaattgg aacgatctct
      901 aaatgatata actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt
      961 ctatcacctg gagagaaaag tgcagaaagt gtgatagcca atccattttg aacaccggaa
     1021 acaatagata gagattctaa ggacggaatg actccttgag tttttaacca atttgctgca
     1081 gtagtcaatt gaaaatttgt gcctttggga tcttggtaat taagcaaacc aacaagattt
     1141 gggttattac tagttgattg aataaacggg acaacgaatt tattacattc ttcaaaagaa
     1201 ctaaccagac caaaatcaat gatatgttcg gttacttcat tggtagagat ggtgatggac
     1261 tcaaagacat tgggagaaat aaatgtgcca cttccaatat tcgtatacag caaacctttc
     1321 tcaattccat atttataagc cttgccaacg gtagtaaaat ttaaatctaa ataatcagct
     1381 agctctcttt gggagggtaa ctgagtgttt ttctgtaatt tattctttaa aatatcttgt
     1441 tctaattgtt gaatcaacga tcgataataa ggacgcttca atttttgttt gtctggtcgc
     1501 caagttaacg ggtaattgtc ataagaatta ataggcattt tctccactcc ttaaaattgt
     1561 ataccataca attttaccat tgattgtatt gattttagaa gttaatcttt tgtgaggagg
     1621 gaattgtcta tgaaaattat taattttgag gaagtagtag cttcttttag ttttgaagaa
     1681 gcgattcaag taatgaaaaa atgttttttt gattatgaaa gtggtaaaat cagccaaaat
     1741 cctcgattgg tggaaatttt acctgacgga aaaggcaaaa atatgtttgc gatgatgcct
     1801 gcttatttgg gggagaacag atattttggc acaaaagtca ttaccgcttt tccggataat
     1861 catcagtatc atttaccttc tcatttagga gagattttct tgtttgatgc tcaaaacgga
     1921 ctacccaaag cattgataaa cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct
     1981 gcgctggcaa cagattactt agcaaatccg catgcagaaa cacttgcttt aattggagct
     2041 ggtcaacaag cattgtcgca tttgactgcg atttctacca ttcgtcccat cactacagtg
     2101 tttgtttacg atattttaga agaaagacga aatactttta tcgctaaagc taaaaaacag
     2161 tatcctaaaa taacgtttat aaatgcaata tcagtagaag atgcagtacg tccagcggaa
     2221 atcatttgta cgttgacctc tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa
     2281 gacgcacata tcaatgctgt gggcacattt actcctgata caagagagat aagtacatcg
     2341 ttgatggaaa cttctcaaat ctatgtagat gattactcag ctgccttaac agaaagcggg
     2401 gacctgctta tagccatggc agaaggagat ttagaggaat cttgtattgt tggtacatta
     2461 ggcgaattag tttctgagaa aaaaacattt tataaaggag aatcgaggcc gacgattttc
     2521 gacgcagttg gtctagctat tgaagatctt tgttgtgcag aatatattta taacaaatta
     2581 gagaaagggg ctttaaaatg aacttagatt attacgtagt tgatgcattt gcagatgagg
     2641 tgttcaaagg aaatccagca gcggtttatg tgctagaaga atggctgcca gaaggtacga
     2701 tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc atttacagtg aagaaaaacc
     2761 aagaatttga gcttagatgg tttacgccag atcgagaaat cgatctttgt ggtcacgcga
     2821 cattagcaac a
//
LOCUS       DASJST010000224         2818 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJST010000224 DASJST010000000
VERSION     DASJST010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.228"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            112..534
                     /locus_tag="U4B05_002829"
     CDS             112..534
                     /locus_tag="U4B05_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051914.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            554..2143
                     /locus_tag="U4B05_002830"
     CDS             554..2143
                     /locus_tag="U4B05_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051915.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2155..2490
                     /locus_tag="U4B05_002831"
     CDS             2155..2490
                     /locus_tag="U4B05_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051916.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2631..>2818
                     /locus_tag="U4B05_002832"
     CDS             2631..>2818
                     /locus_tag="U4B05_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051917.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1120 a          421 c          567 g          710 t
ORIGIN      
        1 aattttcttt tgttcaaagt cataatacaa aagtattaga agaatatatt tgtcagaata
       61 atcaaattat cgaactttta attgaacaaa gtagaggagg agaagtttaa catggccaaa
      121 gttatagaca agcatattta tttttatgaa acacatatag atgttctaga tgaaattatt
      181 gcaactcatg cagaggttaa aaactatagt gaagccgttc ggtttctatg tatgaattat
      241 gattcagaag tggagaaaaa agtaaattcc aatcaagcaa agttgaatgc aatgggaaaa
      301 gaaatttcta ttttaaccga aatggtttca gagtttgcgg atctccattt gaaagataca
      361 gggattttaa ttggtacaaa ttcaactgtt taccaagaag caaaagagcg tgtggaaggc
      421 aagataaaag caaatcaaac gaagaaatat tctcctaaga taaagaaaac aataagcgaa
      481 tcagtgacta caaaaagtga agtatttgat ccaacaaggt tcttaaaacg ataaaaatta
      541 gaaggagaat gtgatgagta aatcacctgc agtcattctc acttgtcaat ttaccttgcc
      601 caataaaaaa tctttttcaa catacattga ttatatgaca agagaaaaag cgctggaaga
      661 aaaggaacga cgtacgcccg aggaagaaca ggaactgcgt ctaatcaaga atgctttaga
      721 ggatttttat attcctgaag gagaaacttt ttcggagagt aagacgggta aagaattcag
      781 cgaaacgtcg aatgaagcgc aatcgttgat gaaacatgga atcaattttg acaagttaga
      841 agaacaagat tttaccaaat atctttctta tatgacacgt cactatgcgt tagaacaaaa
      901 gaaagtactg acgcatagtg aacaaaaaga ataccaacgc ttgaaccaag caataaaaaa
      961 atatcaaaca gagccacaca aaaaagcttc aggaaatctt cctggagttt tttctatggc
     1021 ttcagacgaa gtgaaaggaa gtgatctgaa ggagatcaag caaaccttcc aaagaggaca
     1081 acaaaaaggc tcaatcattt atcaagatgt tgtctctcat gatcattcgt atcttgaaaa
     1141 aataggattg tacgatccca aaacagatac tttagatgaa gaaggtttaa aagcagctgg
     1201 aagaaaaatg atggagacat tgttcgagaa agagcaactg aatgaaacag gctactggat
     1261 ggcaacgatt caccgaaata ccaaacatat ccatattcat tttgcagttg ttgaaaaaaa
     1321 aaatacgcgt aaaccataca ctgagattga aaatggtgtg agttatatcg taccgaaagg
     1381 gaagagaagg caagcaacat tagacgaaat gaagactaat tatgtacatg agttagaacg
     1441 tttttcttat gaaaaaaatc gaatgggtat ggagaaacga aatttattga cacggaaaag
     1501 tgatttgcgt aacaccttaa ccaaagtagt gaaagagcca gtacgttatg atcgacacgc
     1561 aatgagctta ttaactgaag tatataaaaa cttacctaaa aagcgttcgg aatggcatta
     1621 cggggatgaa aatcgaacca aactttcacc atacacaaga gagaaactcg atgagttaac
     1681 ccaatatgtg ttaacaaaag aacctggata tcaagaatat atacagattt caaaagcatt
     1741 gaaagaagaa gcaaaaagtc tttatgggga aagcaagaga gaatcaaagg atgcagaaaa
     1801 aaatgctctc tttgatttaa aaaagcgcac aggaaatgcc attctaacag aactgaagaa
     1861 acaggatagt caaataaaac ctttaataga aaggatggat cagatgcaat ctatttacaa
     1921 tgaacgatcc tttcgaacac ttaaacagtt gccaacgaat gaatacaaat atagttacga
     1981 agaattcatg aagcgcagga aacaattcca acaaagagtt gtcacgcaac ggagtttgcg
     2041 tcaattaaat cgaacgatta atgcccatga agaaaaatac cacgcaagta aagcatacga
     2101 agagcaacaa cgaagaattc aaatggaaca agaaagacag taggaggaat agcaatgaaa
     2161 caatatttag atcaatggaa agttatagaa ggatcattaa gagaagaacg aatcgaacaa
     2221 cttcccgatt gtttagaaaa agagcatttg tttcagataa gagaaatgtt gataaacgaa
     2281 cagtttgatc caaatcaatt tttagtcgtt gagtacccag ctactggtgt gtactgttgt
     2341 aatcatgtga agggtgaaaa atattttatc attcaagaat atgagggcaa gttagcgcct
     2401 tattatacta cttgggaaat gaatgaagag gggatcaata acttcccgtg taaaagcatt
     2461 gaagagtcta tttcactcac agaatgttag ttgttaagtg aataaaagaa gtaaaagagt
     2521 cctgaacgtg attgtccagg actctttttt ttcactgaat tgttttccgc caattcattg
     2581 agtggtgcga tttccgtcta cattatatca aaagaaagga ggaagaagaa atggcttatt
     2641 caacgattgg agttgttgtt gaaaaaagca gagacaatct tgtttttgtg acagaaattc
     2701 aaacaggcag agcttttgta gtcacagaca aagcggcaaa agcatatcaa agtggcgata
     2761 ttttaacgtt aaatctgact acgaagacgt tcgtcgatgc ggcagaagat tatccatt
//
LOCUS       DASJST010000225         2793 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.229, whole genome shotgun sequence.
ACCESSION   DASJST010000225 DASJST010000000
VERSION     DASJST010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.229"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..530)
                     /locus_tag="U4B05_002833"
     CDS             complement(60..530)
                     /locus_tag="U4B05_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051918.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            617..1315
                     /locus_tag="U4B05_002834"
     CDS             617..1315
                     /locus_tag="U4B05_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN2051919.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1493..1834
                     /locus_tag="U4B05_002835"
     CDS             1493..1834
                     /locus_tag="U4B05_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051920.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1827..2498
                     /locus_tag="U4B05_002836"
     CDS             1827..2498
                     /locus_tag="U4B05_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN2051921.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2504..>2793
                     /locus_tag="U4B05_002837"
     CDS             2504..>2793
                     /locus_tag="U4B05_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051922.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGR"
BASE COUNT         1013 a          418 c          563 g          799 t
ORIGIN      
        1 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
       61 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
      121 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
      181 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
      241 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
      301 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
      361 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
      421 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
      481 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
      541 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
      601 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
      661 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
      721 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
      781 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
      841 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
      901 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
      961 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
     1021 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
     1081 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
     1141 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
     1201 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
     1261 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
     1321 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
     1381 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
     1441 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
     1501 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
     1561 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
     1621 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
     1681 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
     1741 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
     1801 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
     1861 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
     1921 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
     1981 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
     2041 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
     2101 gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac
     2161 caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac
     2221 acggactggg attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata
     2281 caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag
     2341 atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag
     2401 ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag
     2461 caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat
     2521 cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa
     2581 tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt
     2641 cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa acttcatcta
     2701 tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct ggaaggaatt
     2761 cttctacttt cgttttgaag gtgtcacagg gcg
//
LOCUS       DASJST010000226         2786 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJST010000226 DASJST010000000
VERSION     DASJST010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.230"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..970
                     /locus_tag="U4B05_002838"
     CDS             83..970
                     /locus_tag="U4B05_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2051923.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="U4B05_002839"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="U4B05_002839"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN2051924.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="U4B05_002840"
     CDS             2272..2718
                     /locus_tag="U4B05_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2051925.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          933 a          450 c          608 g          795 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatt
//
LOCUS       DASJST010000227         2779 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJST010000227 DASJST010000000
VERSION     DASJST010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.231"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(262..810)
                     /locus_tag="U4B05_002841"
     CDS             complement(262..810)
                     /locus_tag="U4B05_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051926.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(811..1668)
                     /locus_tag="U4B05_002842"
     CDS             complement(811..1668)
                     /locus_tag="U4B05_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2051927.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(1954..2241)
                     /locus_tag="U4B05_002843"
     CDS             complement(1954..2241)
                     /locus_tag="U4B05_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2051928.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2231..2560)
                     /locus_tag="U4B05_002844"
     CDS             complement(2231..2560)
                     /locus_tag="U4B05_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2051929.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2637..>2779)
                     /locus_tag="U4B05_002845"
     CDS             complement(2637..>2779)
                     /locus_tag="U4B05_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HEN2051930.1"
                     /translation="SFEKLKPTHEGRYFRRLNRQHRVVYKVDEDEHVVEIYSARTHYE
                     SQ"
BASE COUNT          822 a          559 c          355 g         1043 t
ORIGIN      
        1 atctacatct cctcctaata gttttatttt agagttagct ctattactta tttactaact
       61 caacaacaga ttatcataca cttattaatg aaatcacaat atctcattaa aacacaccct
      121 aaaatcataa ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat
      181 aaatcccctc tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt
      241 tacttagttc ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag
      301 tgagtcttcg gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc
      361 taattctgga acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt
      421 aaacaaatct tgtttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt
      481 tctaagcaat tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt
      541 cgcatttaac aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt
      601 tctgattggt gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga
      661 agtttttatt tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc
      721 ttgctgcttt ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc
      781 aaaaatatca agtcctttga tgtcagccaa ttactccacc tccacatacc aattctcgtg
      841 ttcttccaat tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt
      901 taagagacct ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg
      961 cgcatttttg agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt
     1021 atacatttcc tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac
     1081 atataaattt gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc
     1141 atttctcatc gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat
     1201 cgcggattta ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc
     1261 atcaaataat ggttctagct tttctttcat atatttgaat tgggataatg aatcattata
     1321 ttggtctttg atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag
     1381 gttattgatt actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt
     1441 cacagtattt tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt
     1501 catatgcgtt aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa
     1561 ggttcttttt ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt
     1621 acctacgcca cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct
     1681 tttcttttag caatattcat ttgatactat tataacatat ttatttttag ttactaccaa
     1741 aaaaaagata tatatgtact accttataat taataagtaa aaggtagtac ataaataagg
     1801 aaaagctgaa atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc
     1861 gagaagcaga gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa
     1921 gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata
     1981 aaagttttca tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta
     2041 agctactcta tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc
     2101 cccagttttt tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa
     2161 aaatttttct ttcaatggct tctcttttag cttttttaaa tacttaatga catgttttga
     2221 aggaacgatt tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat
     2281 tagtaagtgt atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct
     2341 taaattctac aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat
     2401 cttcgctaaa atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct
     2461 ctaccaactt tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca
     2521 acatgattga attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac
     2581 agtatgtaat tacttagtaa atacattata ccacgagata atgtatttac aagtaattac
     2641 tgtgactcgt aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc
     2701 ttataaacaa cccggtgttg tctattcaat cttctaaaat acctaccttc atgtgtaggt
     2761 tttaacttct caaatgatt
//
LOCUS       DASJST010000228         2778 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJST010000228 DASJST010000000
VERSION     DASJST010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.232"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            287..694
                     /locus_tag="U4B05_002846"
     CDS             287..694
                     /locus_tag="U4B05_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051931.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="U4B05_002847"
     CDS             708..1109
                     /locus_tag="U4B05_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051932.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="U4B05_002848"
     CDS             1111..1482
                     /locus_tag="U4B05_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051933.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1817)
                     /locus_tag="U4B05_002849"
     CDS             complement(1518..1817)
                     /locus_tag="U4B05_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051934.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1823..2053)
                     /locus_tag="U4B05_002850"
     CDS             complement(1823..2053)
                     /locus_tag="U4B05_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051935.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(2303..2503)
                     /locus_tag="U4B05_002851"
     CDS             complement(2303..2503)
                     /locus_tag="U4B05_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2051936.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT         1015 a          392 c          454 g          917 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacat cagtcacgat atcaaacaaa
     1621 aaatatacgt ctttcttcat tctagttttc gcagcaggga ttttaaagta gttcttatta
     1681 gaatagtaga gattaattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgc
     1801 gtctcattaa aaagcataac cattactccg atctattttt aaacttagtc tctacctcta
     1861 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
     1921 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttgt
     1981 ctttttcgtt gctttgctca taaaaatctg catattcatc actaatattt tttctaataa
     2041 attcttccat cataaagatc acctcgaaaa gagtatacga acaaacgttc tttttgtaaa
     2101 ggtggaattt attatactaa ataaataggt gaacaagtac ttgtgccaaa ttatgtgcca
     2161 aaaaaaatcg aatttaataa aacacaaaca aaaagaaatc ctattatgat aacatttctt
     2221 ataataacaa acacaacaga agacgtgtaa tagttagtca ggaacgtaca aataacccct
     2281 gtatcctttg cggtacaggg ggttattttg tataagtaga cattgtagtt tctaaactgc
     2341 tatgtcctaa acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg
     2401 catgcgtatg tcttagtcca tcaataaaca gtcgaagaga aaatagggaa aaattgatta
     2461 ctaaagaaaa acttactagg tcgacggtgt agttgatttt catacttcaa tagattcgaa
     2521 ttaaacgatt tcgattggct tataccagtc ctatccaacg aaagatatat cgttcttaac
     2581 tctagcggta taacttgaag ctttacgtaa agccaatggg aatgaactgg agatgacttt
     2641 ttattatttt tagtaatagc tgatgggtta atacttacaa ctttcctttt caaagtacca
     2701 tataaaaaaa cggtggggtt gtttagcgaa ataaaggatc taagccaata gcttagatcc
     2761 tttattctat gctttata
//
LOCUS       DASJST010000229         2617 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJST010000229 DASJST010000000
VERSION     DASJST010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.233"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(120..458)
                     /locus_tag="U4B05_002852"
     CDS             complement(120..458)
                     /locus_tag="U4B05_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN2051937.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(474..677)
                     /locus_tag="U4B05_002853"
     CDS             complement(474..677)
                     /locus_tag="U4B05_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2051938.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(692..1468)
                     /locus_tag="U4B05_002854"
     CDS             complement(692..1468)
                     /locus_tag="U4B05_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN2051939.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1767..2087
                     /locus_tag="U4B05_002855"
     CDS             1767..2087
                     /locus_tag="U4B05_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2051940.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2105..2536
                     /locus_tag="U4B05_002856"
     CDS             2105..2536
                     /locus_tag="U4B05_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2051941.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          793 a          460 c          409 g          955 t
ORIGIN      
        1 cttcatcaag ccccataaaa atccaaacca tgtaaacaat cgttcctatc aacgcttgcc
       61 tgcttcccca aagtcctaaa gcgtagatga ttagcggtgc gctgaacact agcgctctat
      121 taaattttcc catccgcttg cctccttaag atttccgaaa aatttgtttc taaaaattca
      181 agtgttttac ttcttaaaaa tagatatgtg tctcttcctt caactggata atagacaaat
      241 ccatttttgt ttttctcgat atcgataata tttctatatc ttgggttctt taaaactcta
      301 gaagtaaacc aatcatattt tctgttaatc cgttctagca cttctggcaa cgtcatccat
      361 ctaccagtat catcagcttt ttttaactcc tcataatcca cttgggagat aattacatag
      421 ccttctggaa ttgggatttt tgcttctaga tattgcatta gctgttcctc cctttacaat
      481 tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt ctttttaccg
      541 tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag catggtgtaa
      601 gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg ttgagtgatt
      661 tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa tccatttgag
      721 ggtaatatcc ttcttttttt agtaattcgt agataaatag acgccctttc tgtgtccatt
      781 tggtattcat tacaatttta gtgccaccat cggctttcgg gatctcagtt gtatgagatt
      841 ttgtgtatcc ttggttcatg tgttttttgc acaataacca ttggttaccg acttttttct
      901 gaatacctag tttatgaagt aatttattca tctgttgtgg agacatccca taatctgctg
      961 caatctgact aattgttact gaatctgtag aagataatat gctatctaaa taggagattt
     1021 taggttcgta ctctgcaatc ttttgttctg cgattaatct tccagttctt tcttctttta
     1081 gttgagttgc taattgaatg attgtatctg gattaagcaa agcttcttct actttttctg
     1141 gagttagata acctccatgt tttctaattg ctggcaacac ttcacttgtt acccaacgtt
     1201 taaatttttt ggcagaggga agttttgatt ttaagattaa actgtacaaa ccagattcgt
     1261 tgatgattgt catctctctt gattgacctg aggtcgtgat tcgcgacccc atcttatctt
     1321 ccaaatctac atgccgcgac aaagcatctt tagtgtttga ataacccaaa acactcgcaa
     1381 cgtctttgcc tacaaaatat ggttcatcat ttactaaaat tgtccgaact tcgttttgtt
     1441 cgaaattaaa aatttgcggt gtgttcatat ttctcattcc tttcttttat attcgtaaac
     1501 aaatttaaca acttttttct aaattcaatt gacagtatct gagtaatact ctatactaag
     1561 agcataatta aataagacat aaaaacattg attttaaagc tttcttggcg gtctgcattt
     1621 acatatcaat agtgtttttt gttgtctttt tagttgttaa atctgtttac aagatatagt
     1681 atagctcatt actcagagtt agtcaaatta tttctgcgta tttttctaaa ctttttttgt
     1741 aaacatttag aaaggttgtt ttatcgatga atacctatga aataataaaa gacttggcga
     1801 agaggaaaaa gatatctata cggcagttag aaattaattt tggatattct aacggttatc
     1861 tagcaaagtg gaaaaataat actcctaatt cagatgaatt aagtcggatt gctgactatt
     1921 tcgatgtctc tgtagactac cttctaggta gagaagaaag agaaacccct aaacatgtgg
     1981 atttatcaga agacgatact gttttttctt ttgatggaaa agaaatatct aaggagacaa
     2041 tgcgtaaagc gattgcaatt gctaaagctt tagaggaaaa tgaatagttg gagtgatggt
     2101 ttgtatgtat ttgaaattga aagaaatgtt gagcgaatac aatttgaaag tagtttatat
     2161 ggagatgaaa gagcctggtt tctactatcc aaaacccaga atcatttttt tgaatgaaaa
     2221 tctatacggt gaaactgcag aagcctttca tttatctcat gaacttgcac atttcagcgc
     2281 ctctcatttt gaattttcag ttttgtacga tacttctaca acttttcact caaaatttga
     2341 aacagaagca gataaaatag ctattctaat tttattaaat atctatattg agaacgaact
     2401 gactgatgaa tctcagttta atctcgaaaa atttatggag tattattcta ttcagaataa
     2461 gctcagatac acttgttatg ctgtctgcca atgctatttt aagaaaaaat attcttacgc
     2521 aaggcaatat gtatgaatac atcaagaatt attaaaaaaa ccgatttcag caaaacatta
     2581 aaaaagcccg tgctgcaaca cgggctcttt cctcata
//
LOCUS       DASJST010000230         2602 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJST010000230 DASJST010000000
VERSION     DASJST010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.234"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..1516
                     /locus_tag="U4B05_002857"
     CDS             137..1516
                     /locus_tag="U4B05_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2051942.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            1744..2602
                     /locus_tag="U4B05_002858"
                     /pseudo
     CDS             1744..2602
                     /locus_tag="U4B05_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          925 a          454 c          553 g          670 t
ORIGIN      
        1 ggtatattct gcaaggagac gattgttttt taaatttagt tcaaatgaac gcgtttcttt
       61 tgattcataa ctaatgtaat gttaagttta aattgtttag aatgatttta aaagaatatt
      121 caaggaggtt tcatcaatgg aaaaaatcaa agttgtcatt gtaggtgcat ctcatggagg
      181 gcatcaatcg atcttggaac tattgacacg atacggtgaa aatgtagata tcacgctatt
      241 tgaagcagga gactatattt ccttcatgtc atgtggtatg gagctgtatt tagaagacaa
      301 ggtaacagat gtaaatgatg tacgtaattt cagaccagaa aactttccgc aatccaatgt
      361 tcatatttta aataatcacg aagtaaaaac gatcgatgcc gataaaaaaa cagtaacggt
      421 tactagcgca tcagataacg aaacaaaaga atatccttat gacaaattga ttttgagttc
      481 tggtgtcaaa ccaaacaatc tgccagtacc aggagcagat ctagaaaatg tctacttgat
      541 gcgtggatac aattgggcaa ccaaaatcaa agaaaaatta aatgatccgg ctattaaaaa
      601 aatcgctgtc gtaggtgcgg gttacattgg gatcgaagca gcagaagcaa gccgaaaagc
      661 tgggaaagaa gtcgtattgc ttgatgtgat cgatagacct ttaggcactt atttagatgc
      721 tgagatgaca gatattttag aacaacacct aaatgaaaac ggtattgaag ttttgacaaa
      781 tgcaaaaatc gaagctttta ctggtaacgg aaaagttgag gcaatcaaaa caagtgaaaa
      841 agaaatccca gcagatctag tcattcaagc tgccggtgtg aaggcaaata cagaatggtt
      901 aaaagggatc gtcgacttag atgaacgtgg ctggatccaa acaaatgaat acttgcaaac
      961 gaatcttcca gatgtgtatg cagtaggaga tgctacactt gcttattcta tcccagcacg
     1021 caaaaaaatg ccgatcgctt tagcgactgt tgcacgtaga gaagcacgtt atgtagtaaa
     1081 acatcttttt gaagaagtac caaacaaacc gttcggcgga gtcgtaggtt cctctgcatt
     1141 aagtgtcttt gactatcatt ttgcagcaag cggattgaat agctttacag cagcaaaagc
     1201 aggagtagat gtacgagtgt cttattatga agatacgtta cgaccaaaat atgtaccaga
     1261 agaacaagga aatccaaaag tgtccatcca attattctcg gatccaatga cccatcaatt
     1321 gctaggagga gcagtattat ctacctatga tgttacaggt caaggaaatg tattggcact
     1381 agcgatccaa aacaaactga ctttagaaga actagcagaa gctgacttct tcttccagcc
     1441 aggctttgac cgacaatgga gcatgttgaa tctagccgct caacaagcgt taggagaaga
     1501 accatttgta gaataagaaa acgaaatatt tgaaaagagg ttgtgaaaaa gctgccttgc
     1561 atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttaact tggctggact
     1621 tattaccgca gatgcaagct tttgaacacc gtttattttc aaaaaaggga ctgtgacacg
     1681 atttgtgtac cgacccccaa aagttagacc aaaaaatcta acttttggag gtcggtattt
     1741 tttatggcta agtatacttt tgaacttaaa ttaaagatcg ttcatgatta cttggatggt
     1801 aaaggcggaa gtgattatct ggcaaagaaa tattccatta aggctccatc acaagtgaag
     1861 cgctggatca atgcctatca agaatttggt gaagagggtc tagtaagaaa gcgacaaaag
     1921 aaaaaatatt ctgttcaatt taagctggat gcgatagagt tatatctaac aagtgagctg
     1981 tcctatcgtg aagttgccaa cacacttaac atgaacaacc ctaatttgat cgctagttgg
     2041 atgcgtcaat tccgtgaggg tgggatcgat ggactctcaa aaacgaaagg atgtccacct
     2101 atcttgtcta agaaaaatga acccaaaaat aagaaaaaat ctacaactaa agcaacgtct
     2161 aaagaacgtg aacgcatcga ggagttggag aaacgagtcc gttcccttca aattgaaaat
     2221 gcctttttaa aagaattgag gaagctgcga aaacaggaag cccaacaacg acgaatgaag
     2281 caatcgcacg aatcatcgca agcctccgag gaccgtttaa attagttgag cttctagaaa
     2341 ctttaaagtt ccctaaggca atctttatgt actggcaaaa acgattggac cgtaaaaatc
     2401 cagatcaaga gattgaagcg gaaatgctta aagttcggga gaagcacaag gattatggct
     2461 gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa aagaaagttc
     2521 agcgcctgat caagaagcta ggaataaaag taacgacata cacacgtaaa tcacggcgct
     2581 acaattctta tcgaggacaa gt
//
LOCUS       DASJST010000231         2595 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJST010000231 DASJST010000000
VERSION     DASJST010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.235"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..434)
                     /locus_tag="U4B05_002859"
     CDS             complement(<1..434)
                     /locus_tag="U4B05_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2051943.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEV"
     gene            complement(444..1235)
                     /locus_tag="U4B05_002860"
     CDS             complement(444..1235)
                     /locus_tag="U4B05_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2051944.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(1228..2001)
                     /locus_tag="U4B05_002861"
     CDS             complement(1228..2001)
                     /locus_tag="U4B05_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2051945.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(2157..>2595)
                     /locus_tag="U4B05_002862"
     CDS             complement(2157..>2595)
                     /locus_tag="U4B05_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2051946.1"
                     /translation="ENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRF
                     LDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEG
                     AQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT          835 a          522 c          357 g          881 t
ORIGIN      
        1 acttcaactc catcactaat tagttcttta aaagcctctc tctcttcttc agtaatattt
       61 atcgtcggac ggatgcgttc tgttccattc cttcttgata aatttcctac gtttacactt
      121 gttattggta aaccgttatc aatcaaaaat ttaacatcga ttggagaaac tgcaacaata
      181 agaacttttt gtccttcata tttaccagaa agaatatttt taactgctac ttcccttgta
      241 attattgaag ttctaacatt aggtggtgca gcgaggcgaa ctacagcttt ttttacgtca
      301 tcattggcta cttcattatt aataaccata attctgttag cactcaattg atttgtccat
      361 tgtgttgcta cttgtccatg aatcattcga tcatcaatac gaacatgtat aatacctttc
      421 attttttcat ccatattcaa cacctacttc aaaatattta acgctcttaa cgcaatagaa
      481 actactaaga ttaatagaat tacataaact ggtttaacct ttcgtcctaa caaataatac
      541 acaattgcta ctacacatgc aggtaataaa ccaggcataa cttgatcaaa gatttcattt
      601 ccggtcattg ttaagtcgcc ttgtttaaat tcaaagccaa cattcacttt tacaactgtg
      661 gcaatgagtg caccgaccac cattaaccct aaaattgata ctgcatcagt aatagacttt
      721 aattttgttt caatttgagt tagtaggctc gcacccgatt ctttcccagc aaggtataaa
      781 ggataagcag taatccttaa taaaattgat gccagtatcc acaaaataat tccaacaaaa
      841 ctaccttcca atgccatatt agctgcaatt gctccaaaaa tagtccatgg aataactaca
      901 aatacactat ctccaattcc cgctaaagga cccattagac ctgttttcag agcacttact
      961 gcttccaaag acttattttt ttcttctttt tcaattccaa cattcatacc aataatgtaa
     1021 ggcgctgtat acggcgtagt gttaaaaaat tgtaagtgag ctaaaacgga ttgtttcatt
     1081 ccttcaggat catctttata tgtgtccttg ataaaaggaa gcatagaata cgcataaccc
     1141 aacccttcca ttttttcata atttaatgaa gttgcaccat tgagaatcca ccggaaaatt
     1201 acttgtttgt cgatttttgt cagtttctta ttcatcttcg tcaaaacctc ctgttgcagc
     1261 tggtgctact tgagtattat ttgatctatt tttatatacg attagtgcga ttgcagcgcc
     1321 aagtaacgaa atccctaaaa ttggtatttg gaaataaact gccaaaacga aaccaactaa
     1381 caaataggca aagtaatctt ttgtaggtaa atatttaagt agcattccta tcccaacagc
     1441 aggtaatagt ccccctgcta cctttaaacc accggtaacc cattcaggaa tataattgat
     1501 tgcacttccc actaacgttg ggcctaatat cacagttaac aaaacaggaa ttccagatac
     1561 aaagcttgta ttaattgatg ccaaatattg catccactct aatttttgat aatcttcttt
     1621 ttgtgcgaat ttttctgcct tttgttgaaa aaaaatgttt gttgaccact ttagaacatc
     1681 tagttgtaca attaacattg ctaccggtat tgctaaagtt attcctatag aagcatcctg
     1741 gttagttgag attgctaaat aagtaccgat caatgctgct gtttgataat ccggtactga
     1801 tgcaccacca aaactagtaa tacctagtgt cattaattgc aaagtcccac cgatataaag
     1861 tccagtttga atatctccca taataagtcc ggtcaagaaa cctgcaacga caggttggga
     1921 ggttccgatt ttgggccctt ccttatccca gttaatgaaa aatccataca acacaactaa
     1981 aaatgtttgc caaaacgcca ttttgttctc tcctttcttc ttatactttt atataagcaa
     2041 ttactgtgcc aactaacatt atccaataaa gcgggaaaat acagtaaata ttaattaaat
     2101 gagcgtctaa atatactttg gacacccttt cattttttta gacgctagta tgctatttag
     2161 aaaatagtct gctcaaaaat tgtatagatt aactcatact ctcctttggg tatttcaata
     2221 cagtaaatat ctttgatttc tgaaaaaaaa tcttttgata catttaaaaa agtatcaaat
     2281 agttgcgctc cctctttcaa atgttcattt gttattccat caccaatcat aattcgttca
     2341 atcattccag aaagatgtag aaatagatta attctcagaa aacttgggat tttgacatcg
     2401 taatattttt catacaaata tagtactttt tctatctcat tgaatacttt tttggaatct
     2461 aaaaagcgaa gcttagtact agcgccttca atagtaaaga gtttaactaa ttcatttaag
     2521 cagaactcac agctatccga gtccatatac ttatttaatt tttctaaggt ttgattccca
     2581 ttaattagat tttct
//
LOCUS       DASJST010000232         2577 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJST010000232 DASJST010000000
VERSION     DASJST010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.236"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..2514
                     /locus_tag="U4B05_002863"
     CDS             73..2514
                     /locus_tag="U4B05_002863"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN2051947.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          861 a          459 c          610 g          647 t
ORIGIN      
        1 aaaaaataac tggaacaaga gctatttgtt tttacagcaa agaatggtat atttttgtag
       61 gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc gataggaaag
      121 tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt gactaaagga
      181 ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc
      241 ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag cggtctccct
      301 gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc
      361 ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg cggaacagag
      421 agtatgtctc aagcacctat gctgaaacag tatcagtcag aaacaaatga atatggtgaa
      481 ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc acatatggga
      541 ttaaccgcag agaatgttgc aacacaattt tctgtgagca gagaagagca ggatcgctat
      601 gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt ttctgaggag
      661 atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc agttcgtgga
      721 aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga aggaactgta
      781 acagcaggaa atgcttcccc gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa
      841 gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt agcggaggtc
      901 ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa attgacagat
      961 agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc atttgcagct
     1021 tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa tatatatgga
     1081 ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt gactacatta
     1141 gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg tattggtgga
     1201 ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa ggatgtgcaa
     1261 aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat agaaaaaaat
     1321 gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga agagctctcc
     1381 gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatggggat cgcgcagaat
     1441 tttcaaatca atggcaagaa aaaatggatt ccgatggcaa cagaggaacc atctgtcatt
     1501 gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg
     1561 ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt gatcaatgca
     1621 gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg
     1681 aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag tttccatgca
     1741 tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa catgatcaac
     1801 tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga agagattcta
     1861 ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg tgaaatccca
     1921 ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag
     1981 gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa agggatcatg
     2041 aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg
     2101 atccacgcct acgcggcaag aaatggatta taccaaggat taacagactg gcagatcaaa
     2161 ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt aggcggagct
     2221 tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga ttcagccaag
     2281 gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca
     2341 ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc attagcgatc
     2401 tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg tgaagcagaa
     2461 aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc
     2521 aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca gatcttg
//
LOCUS       DASJST010000233         2560 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJST010000233 DASJST010000000
VERSION     DASJST010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.237"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(145..840)
                     /locus_tag="U4B05_002864"
     CDS             complement(145..840)
                     /locus_tag="U4B05_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2051948.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            994..1272
                     /locus_tag="U4B05_002865"
     CDS             994..1272
                     /locus_tag="U4B05_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051949.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1291..2499
                     /locus_tag="U4B05_002866"
     CDS             1291..2499
                     /locus_tag="U4B05_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2051950.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
BASE COUNT          765 a          457 c          442 g          896 t
ORIGIN      
        1 caggctcaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
       61 aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca ggtcaaaacg
      121 ctgatttcac aacctctttg ttcatcaaaa gataatattg tcttctaaca agtccatatt
      181 caaaataacg atatgatggt ctttaacggt gataatacca tcttttttca gttgttttaa
      241 aattctgttt acactgctag cagttgagat gccacaaaat ttggcgattt cttcattcgt
      301 gatcgaaaaa tcgatcagca tcccttcttc tgtccgcaca ccgaataaat catataattc
      361 gtaaagttgc gtacaaacag ctcccagctt tccgtttaat agcatttgct gcattttttt
      421 catcgaataa agcagacgat agcggtaata ctcttttaca tagtgttgta gctttgggtc
      481 ttggttgata ttttgccaaa agcgcacacg attgattcga taaaactctg catattctga
      541 ttcaactcga atattatacg gcgaatcgat gaattgtgaa tattcgtctt ttaaaagaga
      601 gacgatttca agatctttga tatagcgaag gttaaattct ctcccatcat ttgaaataac
      661 acttgctttt accgttccct ctttcaagat atacgtatgg gaatcttcca tcccttcgaa
      721 tgtcaaatat ttatgatatc ctttgcgtat aatcggaaaa tcatttttat ccaaatattc
      781 ctttaagact ctactatcca atgatgctcc cccatccaaa ctttcatcta aaaagatcat
      841 acagtacttt aaaacttttt aaagtccaaa ggtattttac taatgatatt aacatttgtt
      901 aagtaccttt ttccatcact tatgcgattt ttttcctttt ttttattcgt tatactgcag
      961 aagtaaaatt ttttaagaat agagggaaaa aacatgaatt actggatgaa cacaatcatt
     1021 aatcgattag aaacagcata tcaaacaaga tttgatatga aagcttcttt agtcttttta
     1081 aatgatgcgt atcaaaactc gatcgaattg atcaaagcag tggatgagaa tccaacaaac
     1141 gaatgcgaag aatttttaaa tctgtttatg tctacaagag atttatttat tcgccaactt
     1201 gttgatcgct atccatccaa ttatcacgat gtagaagttc aaatacaaaa attaaaagca
     1261 tattcagctt aaggggaatg tataacatac atggaaaaga aattatttaa taaatatttt
     1321 attggaatca cattgatcaa ttttattgtc tacctgattt attatctgtt gatggtcatc
     1381 atggcagtca ttgcgcaaga caccttacac gccagcttga gccaagcagg ttttgcttct
     1441 gggatctata tcatcggaac attgctggca cgcttattca tggggaaaca gttagaacta
     1501 tttggtcgga agtttacctt aagaggcggc gcgatcttct atctcctttc aacattagca
     1561 tatctttata cgccaactat cggggcattg tatttagttc gattcttgaa tggattcggc
     1621 tacggtacag tttctacagc tacaaatgct atcgttactg catatatccc tgcatctaaa
     1681 aaaggggaag gcatcaatta ttatggatta agtactagtt tagctgccgc tgttgggcca
     1741 tttttaggaa tgattttgct gaatatgact gattttcgtt tcattatctg gttttcaatt
     1801 gtactggtct tctttgttac gattgcctgc cttgtctttc ctgtaaaaaa tattccatta
     1861 tctgaagaac aaaaaatatc tcttcgctca tggaatatca atagttttat tgaaaagaaa
     1921 gcacttttca ttacaggaat tgcctttttg atgggtcttt cttattcaag tgttttatca
     1981 tttttatctt cttatacaaa agtaatccac ttagttgctg ctgggtcatt tttcttcgtc
     2041 gtatatgctt tggtcatcac atttacacgt ccattgactg gaaggatttt cgatgtcaaa
     2101 ggagaacaat acgtgatgta tcctagctat ttattcttaa cagcaggatt atttttactg
     2161 agtgtaacga cgaatagtgt gacattgctt atctctggag cattagtcgg cctaggttat
     2221 ggtacgttta tgtcaaatgg acaggctgtt tgtttaaaaa tcgtccgcga acatcgaatc
     2281 agtattgctt tatctaccta ctttattggt ttagacctag gcttaggcgt tggtccttat
     2341 ttgatgggga tgctaaaatc tcttacttca tttagaggat tgtatgtgat tgccggagtc
     2401 attccattga tatgtacagc actctatctt gttaatggcc gaaaacatag tcatgaagca
     2461 gagggcgaat tgattgaatc attgaaaaat gaaatttgat cttgatgaat agataaaaaa
     2521 aaccgttcag atctgaaaat aatctgagcg gttttttgag
//
LOCUS       DASJST010000234         2501 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJST010000234 DASJST010000000
VERSION     DASJST010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.239"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            350..949
                     /locus_tag="U4B05_002867"
     CDS             350..949
                     /locus_tag="U4B05_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2051951.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            998..1714
                     /locus_tag="U4B05_002868"
     CDS             998..1714
                     /locus_tag="U4B05_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEN2051952.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1728..>2501
                     /locus_tag="U4B05_002869"
     CDS             1728..>2501
                     /locus_tag="U4B05_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEN2051953.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLN"
BASE COUNT          902 a          372 c          487 g          740 t
ORIGIN      
        1 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
       61 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
      121 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
      181 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
      241 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
      301 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
      361 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
      421 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
      481 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
      541 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
      601 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
      661 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
      721 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
      781 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
      841 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
      901 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
      961 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     1021 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     1081 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     1141 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     1201 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     1261 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     1321 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     1381 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     1441 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     1501 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     1561 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     1621 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     1681 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     1741 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     1801 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     1861 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     1921 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     1981 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     2041 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     2101 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     2161 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     2221 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     2281 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     2341 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     2401 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     2461 ttttgtgcga gtatgtgttt actaagtata aaggattaaa t
//
LOCUS       DASJST010000235         2482 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJST010000235 DASJST010000000
VERSION     DASJST010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.240"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..2332
                     /locus_tag="U4B05_002870"
     CDS             179..2332
                     /locus_tag="U4B05_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN2051954.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          850 a          428 c          546 g          658 t
ORIGIN      
        1 ttatttttta atgaaaaaaa tagttggata tgtaacttta agtgattgct gtgaaacaaa
       61 aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt ttttcttttt
      121 tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag gataggaaat
      181 gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa taagaaaagg
      241 tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc gcttaaattt
      301 tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg gttatttgca
      361 gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga ttacggtcca
      421 agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag ttacgaataa
      481 agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag atttgcttga
      541 aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc tgacagatag
      601 agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca caaaaagact
      661 ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg tcaataaagt
      721 cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca tctttaaacg
      781 gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg ttactgatca
      841 agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga ctggcacaga
      901 ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca gtgtgacgac
      961 tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag gttattctcg
     1021 gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc tgcaaggaac
     1081 caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc aaaaagaaat
     1141 cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat tccaatcaaa
     1201 agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca aagcacagta
     1261 ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc tagcaatgac
     1321 tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag gcacgattac
     1381 tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag gctgggcaag
     1441 taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc aaggtgcttc
     1501 agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag tcaaagcatc
     1561 ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag gactagacta
     1621 tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc agcaattgag
     1681 agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc cgaatgaatc
     1741 tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata atggcaaaga
     1801 atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca catatacacc
     1861 aatccaatta gctcaatatg cttcaacagt cgctaatggc gggaaacgag tacagccgca
     1921 cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag tgaagaaaga
     1981 aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg cgatcctccg
     2041 tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca aaccattagc
     2101 cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg cggaaggtca
     2161 tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag acaatccgga
     2221 aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca taaacttgac
     2281 gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat aaaaaagagg
     2341 tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat agcttctcac
     2401 atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg tttactcggc
     2461 aaaaaaggtt gtgacaaaaa tt
//
LOCUS       DASJST010000236         2474 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJST010000236 DASJST010000000
VERSION     DASJST010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.241"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..417
                     /locus_tag="U4B05_002871"
     CDS             <1..417
                     /locus_tag="U4B05_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2051955.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            631..1326
                     /gene="vanR"
                     /locus_tag="U4B05_002872"
     CDS             631..1326
                     /gene="vanR"
                     /locus_tag="U4B05_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN2051956.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1304..2458
                     /locus_tag="U4B05_002873"
     CDS             1304..2458
                     /locus_tag="U4B05_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2051957.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          853 a          443 c          521 g          657 t
ORIGIN      
        1 caggaagatg acatcattta tgttacagac ttaactcgaa tcactcgtag tacacaagat
       61 ctatttgaat taatcgataa catacgagat aaaaaggcaa gtttaaaatc actaaaagat
      121 acatggcttg atttatcaga agataatcca tacagccaat tcttaattac tgtaatggct
      181 ggtgttaacc aattagagcg agatcttatt cggatgagac aacgtgaagg gattgaattg
      241 gctaagaaag aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa tcacgcagga
      301 atgaattatg cggtaaagct atataaagaa ggaaatatga ctgtaaatca aatttgtgaa
      361 attactaatg tatctagggc ttcattatac aggaaattat cagaagtgaa taattagcca
      421 ttctgtattc cgctaatggg caatattttt aaagaagaaa aggaaactat aaaatattaa
      481 cagcctccta gcgatgccga aaagcccttt gataaaaaaa gaatcatcat cttaagaaat
      541 tcttagtcat ttattatgta aatgcttata aattcggccc tataatctga taaattatta
      601 agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat tgtggatgat
      661 gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta tacggttttc
      721 aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat tgaccttgcc
      781 atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa aataagggac
      841 aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga taaaattaca
      901 gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact ggagttaatt
      961 gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa ggagcagaac
     1021 gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga gtgttatctg
     1081 aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat cctctgtgaa
     1141 aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg cgacgaatat
     1201 ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga aaaaatgaac
     1261 gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta taaaattgaa
     1321 aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata tcgttgcaat
     1381 tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag ggaaacttgg
     1441 ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg acgcgatgaa
     1501 attatatcaa tattccatac ggaacaatat agatatcttt atttatgtgg cgattgtcat
     1561 tagtattctt attctatgtc gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat
     1621 aaataccggc attgatgtac ttattcagaa cgaagataaa caaattgagc tttctgcgga
     1681 aatggatgtt atggaacaaa agctcaacac attaaaacgg actctggaaa agcgagagca
     1741 ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat
     1801 taaaacgccc cttacatcca ttatcggtta tttgagcctg cttgacgagg ctccagacat
     1861 gccggtagat caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt atcgactcga
     1921 acagctaatc gacgagtttt ttgagattac acggtataac ctacaaacga taacgctaac
     1981 aaaaacgcac atagacctat actatatgct ggtgcagatg accgatgaat tttatcctca
     2041 gctttccgca catggaaaac aggcggttat tcacgccccc gaggatctga ccgtgtccgg
     2101 cgaccctgat aaactcgcga gagtctttaa caacattttg aaaaacgccg ctgcatacag
     2161 tgaggataac agcatcattg acattaccgc gggcctctcc ggggatgtgg tgtcaatcga
     2221 attcaagaac actggaagca tcccaaaaga taagctagct gccatatttg aaaagttcta
     2281 taggctggac aatgctcgtt cttccgatac gggtggcgcg ggacttggat tggcgattgc
     2341 aaaagaaatt attgttcagc atggagggca gatttacgcg gaaagcaatg ataactatac
     2401 gacgtttagg gtagagcttc cagcgatgcc agacttggtt gataaaagga ggtcctaaga
     2461 gatgtatata attt
//
LOCUS       DASJST010000237         2404 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJST010000237 DASJST010000000
VERSION     DASJST010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.242"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..356
                     /locus_tag="U4B05_002874"
     CDS             <1..356
                     /locus_tag="U4B05_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN2051958.1"
                     /translation="SPRETIGVIDKNALSMAVNQPKQHIFGKDLYPDKFSKAAVLLIN
                     LIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVVEFVVEIATYQGDFDDLKEAT
                     TDILKKNSTKTDSKK"
     gene            875..1825
                     /locus_tag="U4B05_002875"
     CDS             875..1825
                     /locus_tag="U4B05_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEN2051959.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(1822..>2404)
                     /locus_tag="U4B05_002876"
     CDS             complement(1822..>2404)
                     /locus_tag="U4B05_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2051960.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT          845 a          361 c          424 g          774 t
ORIGIN      
        1 attcaccgcg agaaacaatc ggagtcattg acaaaaatgc gctgagtatg gctgtaaatc
       61 aacctaaaca gcatattttt ggaaaagatc tgtatcctga taaatttagc aaagcagctg
      121 ttctgctaat aaatctgatc aaaaagcatc catttcataa tgctaacaaa cgtacagcat
      181 ttttagccgc gtatgttttt ctcaagctaa atggctattc actaaaaatg aaaaatcagg
      241 aagtagtaga atttgtagtt gaaattgcta cgtatcaggg tgattttgat gacttaaaag
      301 aggcaacaac agatatacta aaaaagaatt caacaaagac cgattcaaaa aagtagaagc
      361 aaactaggga ttcagttaaa tctgaagatt tagtttgttc tttattttag tttacataag
      421 atacattatg cgaagttcat tattttaaaa gcttaaataa taaacttttt tataatacca
      481 atttatttat gtaaaccaat gaccttaatc gcttaagcaa gcatgttatg taatactcta
      541 ctctctccaa aactaatgac tatctatttt caaagaagct agaatgatta tctgatcaaa
      601 tgataattta tatttaattc ctggtctact gctcttcttt taatcaatta atgtctaacg
      661 tactgtacac gataaagtag gaagacttat tttcaacttc ggattcattt ttttaattcg
      721 gtgaaaatca ctaaattaag agtttatcca agttcatttt agctgagaat acgcttgaaa
      781 ataattttga tgaattttcc tgaattaaaa tttattaact tgttgagtga tataattgta
      841 atgtattacc aaatagttag cacttagatt agtgatggaa aggaatagtg agaacaagat
      901 gactggtaga aatcccaaag tatttatatc atattcatgg gagaataagg aaaataaaga
      961 atgggctaga gaactagcag acaaattact tgaaaacggt attgatgcat atatagatca
     1021 gtatgattta agtttaggtg atagattacc tcagtttatg gagcagcaaa tatccgattc
     1081 agattatgtt ctaattatat gtacaccaga gtataaaaaa aaggcaaata atcgtacaag
     1141 tggtgttggg tatgaaggtc atattatctc agcagaattg ctttctggaa aaaatgaacg
     1201 taaatttatc ccaataatta aagaaggacg tgcaagcgaa gtacttccta cttttcttgc
     1261 tggaaagctt ggaattgatt tatcagatag ccagcattat gatgaaaact tagatgattt
     1321 aattacaact atatttggtg ttaagagaaa accagcatta ggtgatatac ctaggcatat
     1381 taaaggcagt cctttactac ctatagaaca taatgaaaat aaagatattg aaatcttagg
     1441 aattatcact gataaagtaa ctgtacctaa aatggatggc tcaaggggtt ctgcattata
     1501 taaaattcca tttagattaa atagagtacc aagtcagctt tggaaagagt tgtttgttag
     1561 aaattggaat tcaccaccac gatatacaac aatgcataga ccaggaattg cttctgttgt
     1621 agacgatgaa attttactta atggaacaac tattgaggaa gtccgggaat atcatcggga
     1681 aactttaaaa ctttgcattg aaaaaagtaa tactcaagaa aaaaaaatat tgctagagga
     1741 ggaacaaaaa aaagagagag agaatgagcg agtcagatca cataaagaaa gtgttcagaa
     1801 attagcagat gaattagagt tctagttgat agtcagttct ttcttaattc gatacacagt
     1861 ttgtctagtt acattatagt ctgttgctat ttttgaaata gcaacaccat tctcctggtc
     1921 ctgaacgatg cttctataaa caaggcgacg ttgaggatct tttgtgttcg cactgtataa
     1981 cttaggacgt cctttataga cgccgtttgc tttagcaatc tttatccctt gtgcctggcg
     2041 tcgttttgat tcagtacgct cttgttcagc aatcatggcc aagatttgaa tgattaggtc
     2101 tttgataaat ttatccagga gaggatttcc gattgcttca gccatgatcg gtaaattagt
     2161 gataataagt cgcacatttt tcttctttag ataatttact gaatcgataa tctcgtcata
     2221 attacgtcca agtcgatcga tagcctctac aataaaaata tcttggtctc taacaaaatc
     2281 tagaacttcc tgaaaaatcg gtcgatgagt aatagtcgct ccagattgtt tctcgatgaa
     2341 aattttttca gcaccaaatt ttctcatttc ctcgatttga cgttgttcat tttgatcgat
     2401 cgat
//
LOCUS       DASJST010000238         2401 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.243, whole genome shotgun sequence.
ACCESSION   DASJST010000238 DASJST010000000
VERSION     DASJST010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.243"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            208..2070
                     /locus_tag="U4B05_002877"
     CDS             208..2070
                     /locus_tag="U4B05_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2051961.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2401
                     /locus_tag="U4B05_002878"
     CDS             2070..>2401
                     /locus_tag="U4B05_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2051962.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2401 a
//
LOCUS       DASJST010000239         2317 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJST010000239 DASJST010000000
VERSION     DASJST010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.244"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..783
                     /locus_tag="U4B05_002879"
     CDS             22..783
                     /locus_tag="U4B05_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2051963.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(898..1338)
                     /gene="fabZ"
                     /locus_tag="U4B05_002880"
     CDS             complement(898..1338)
                     /gene="fabZ"
                     /locus_tag="U4B05_002880"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2051964.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            1558..2313
                     /gene="fabI"
                     /locus_tag="U4B05_002881"
     CDS             1558..2313
                     /gene="fabI"
                     /locus_tag="U4B05_002881"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN2051965.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT          764 a          439 c          446 g          668 t
ORIGIN      
        1 gtaaattgga aaggaacagt tatgaagaaa aataaagtta ttttaagtat tagtttagca
       61 gcagcattgc tgtttgctgc tggttgttct gcaaacaaca cgacttcttc tagttcgtct
      121 acagaaagtt caaaaagtac atctactgcg gaaagcacga ctgccagtag cgtcgatttg
      181 aacgcattgg aattaccaca attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa
      241 atggtgacga ataaaggcac aatcaaaatc aaattatttc ccgaacacgc accaaaagcg
      301 gttgaaaact ttatgacaca tgcaaaagac ggttactaca acggactgac tttccatcgc
      361 gtcatcaaag atttcatgat ccaaggtggc gatcctaaag gcgatggcac tggaggggaa
      421 agcatttggg gagaaggatt tgaaacagag atcaacaacc acctttataa tataagaggt
      481 gcgttatcta tggctcgctc acaagatcct aacagtaacg gcagccagtt ctttatcgtt
      541 caaaatacag atgatatgca tgatggtctt ttaaaagacg actatcctca agcaatcatc
      601 gacgcttata aaaacggcgg ttatccttca ctagatggcc attatactgt cttcggccaa
      661 gtgattgaag gaatggatgt cgtggatgct attgcaaata tcgaaacaga tagttcagac
      721 aaaccaaaag aagaagtaaa aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa
      781 taaaaaaata acgtctgaat cctgcataga tgacaacgta tgctaggatt caggcgtctt
      841 ctttttacat aaaaagcaag taagcttcct tttgttttgc tatatcaggt tttatagtca
      901 agcattttgg ttgacgatga acgtgaactc gcactcacag acttttttat catctacata
      961 tgctgcagat aacgctgtac cgactgatcc cttcattttg gtgatttcaa aatgacattt
     1021 cagcacatct cctggaacaa ctttttgacg gaatttggcc ttattgatcc caccgatata
     1081 agctcttttt cctagaaact cttctgattt taggataagt atcgaacctg cttgtgccag
     1141 tgtttctaga atcaacgcac ccggcatcac tggattgtca ggaaagtgac cttggaaaaa
     1201 atcttcattg attgttacat tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc
     1261 tacataatca atataacaaa taggataacg attaggaatc aattccatta cttcagtagc
     1321 agataatacg taagacataa ttaaactcct tctttcaggc aattgccatt ttcattactt
     1381 tgactatcaa agtatcatat tcctaaatat ttagcaatct ttttttaatt taagaaaaaa
     1441 tagtttgaca atcaaaacat tttgactaat aattaagaaa tcaagaagtt attttagcta
     1501 ttattctcta tttttttacg actatcaaat ttttatcaga aacaaaggag agtcattatg
     1561 tcatttctag aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct
     1621 tggggttgtg ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat
     1681 gaacgaatga aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt
     1741 gatgtttcta gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg
     1801 gagatccacg gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat
     1861 gtgtctgata tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta
     1921 attgctgtat caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg
     1981 acttatctag gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct
     2041 tctcttgaag ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc
     2101 aatggaatct cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa
     2161 aaactgatcc agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa
     2221 gtcggtaatg cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt
     2281 atctacgttg acaaaggtgt ccatctttca taaactt
//
LOCUS       DASJST010000240         2294 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJST010000240 DASJST010000000
VERSION     DASJST010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.246"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..392
                     /locus_tag="U4B05_002882"
     CDS             <1..392
                     /locus_tag="U4B05_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2051966.1"
                     /translation="HGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRENIEKIVS
                     QVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLRLQGKKSD
                     DIIENLRNMNTIACLADMQRHTNEEDE"
     gene            396..1796
                     /locus_tag="U4B05_002883"
     CDS             396..1796
                     /locus_tag="U4B05_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEN2051967.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            1819..2193
                     /locus_tag="U4B05_002884"
     CDS             1819..2193
                     /locus_tag="U4B05_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2051968.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT          834 a          284 c          475 g          701 t
ORIGIN      
        1 ctcatggaaa tattgccatt ggatttgtag atacattaaa aatgtttttt ggcgataatt
       61 taaattgtca tgtggtaaca cttggagatg caggcattga agaattccgt gaaaacattg
      121 aaaaaatagt ttctcaagta aaaaatcaag aagttatggt atttgctgac ttgattggtg
      181 gaacaccttt caacgaattt gctaaaagaa gtgaagtatt tcaaaatggg tttcaacttc
      241 ttggtggcat gaatttagga attttgattg aggctgtcaa tttgagatta caaggaaaaa
      301 aaagcgatga tatcatcgaa aatctaagaa atatgaatac aattgcgtgt cttgcagata
      361 tgcaaagaca tactaacgaa gaggatgagt gataaatgca aaaaattttt tacaagccta
      421 aagatttttg gtttggggat tgcatgcctt tttataaaga tggaactttt tatctatttc
      481 atcaacgaga tacgagaaat cctaaacctt taactgatcc ttttgggtgg tcactagtta
      541 caaccactga ttttgtgaaa tttgaagaat acggggaagt aattcatcgt ggtggcaacg
      601 atgacattga tcaatacatt tacgctggaa gtattgtcca aggaatcaat gagtatttag
      661 ctttgtatac tggacataat cgcaaagcta agttggatgg aaaaacttca gaagtgttaa
      721 tgattgcaag ttctaaagat ttaattcatt ggaaaaagga tggcgaagca gcaaaacttg
      781 ttcctcaaga aggatatgat aagaatgatt ggcgcgatcc ttttgcaatt tttgatcaag
      841 atcatggaaa atacgttttg atactaggag ctagaaaacc agcaagtaag aaaaagccga
      901 cggggagatt ggtctatttt gaatccgaag atttaaaaga ttggaatttc aaaggagact
      961 actgggctaa taatgaattt aacatgattg aaatgcctga tatatttgaa attgatggaa
     1021 cgtggtattt acttttttca gagtattgtg aagataaaaa gactaaatat cgagtcggag
     1081 caggattatt ttcagattgg aaatcaccag tagacgaagc tttcgacgga aaagcctatt
     1141 atgcagcaag aacagtcgga actaatgaaa ctggccgttt tctaactggc tgggtggcaa
     1201 caaaagaagg ggagaacgat caaaataact gggattgggg cggtgcactt gttccacatc
     1261 aaattattca aaagacagac aaaactcttg gtgttaaact gcctgaaaaa ataattgaga
     1321 attttaaaga aatcactcca ttttcaagtt ttaagttaga gaaagaattt ggaaaaaaag
     1381 aaagagtaat tgttcctaaa atcgaagatt attttctatt tgaaacaact ttgactatta
     1441 catcgttaac aaaggagttt gctttaaaat tatttgtaga tagtgatact caagaaggat
     1501 acgaatttcg tatttcattg attaataatc agcttacttt cggaaaaact ccgaactatc
     1561 cttggcctca aatgtttgat aaggggctgc aacgaccact atttttggaa ataggcaaag
     1621 aatatgaact aaagttaatt gttgatgata cgatagctgt tttatatatt gataatgttg
     1681 ctttaagtac tagaatgtat gataaagtcg gaacagcatt aagtatgact gttacggagg
     1741 gagcaatcaa ggtagataaa ctagactatt caataaatta tgaatgtgga ttttaaaaca
     1801 attgaaggga tgatgtaagt gaaattaaat ggtttacaac atattggaat tccaacaaaa
     1861 gattttggag cttcaaaaat cttttatgaa tccttagggt ttagtctgat taatgaagaa
     1921 atgaatgtgg gaagtaaagt cggctttttc gagttgaatg gaacgataat agaaatatgg
     1981 gaagataaaa ctactgagtg tagaggagcc attgatcatc tagcattaga tacggatgac
     2041 atagatgagg tattcaattc aattcaagat ttaggatatg aattattaga taaaaatatt
     2101 atggaattac cgttctggga aaatggaatc cgttacttta atttctacgg accaaatcat
     2161 gagattatag agataagcca aatgaagaaa taaatagata acgaagtgct ctaagttggt
     2221 atgaacgaaa agtacttcat tttttggtat cagcatttca atagtttaat gagaatttac
     2281 tatttcaaat taat
//
LOCUS       DASJST010000241         2292 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJST010000241 DASJST010000000
VERSION     DASJST010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.247"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            264..518
                     /locus_tag="U4B05_002885"
                     /pseudo
     CDS             264..518
                     /locus_tag="U4B05_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            588..1100
                     /locus_tag="U4B05_002886"
     CDS             588..1100
                     /locus_tag="U4B05_002886"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2051969.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1097..2134
                     /locus_tag="U4B05_002887"
     CDS             1097..2134
                     /locus_tag="U4B05_002887"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2051970.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          829 a          418 c          462 g          583 t
ORIGIN      
        1 ttttgattat gtaataagtt ttggtactaa aaaagacttg atctaattag accaagtctt
       61 ttgttagtgt tatattaata acaaaataaa aataagaagt cgctcactcc ccgaccaaag
      121 tttgtgagca aaataaacag tcgatgtttg tttcttaatt gtatatcttg atattaaacg
      181 atttaagatt attcttcaag atatatattc gcaaatgtaa ttggaaaagc gacttcttaa
      241 acaaaattaa ggagtcgctt tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg
      301 tggaaaatta cacaaaaaaa ccggcgtact ctaatagccg gttagacgca catttcgtgt
      361 gcacttctaa tccgaaattg agctttgacg ctatgacgat agtcggaaac cttaataaaa
      421 acagcgctaa aaagctatcc gattttatga gtttagatcc acaaattaga ctttgggata
      481 tacttcaaac aaaatttaaa gctaaagcac tacaagaccc cctatgttag gctagttgtc
      541 gtataggata ataagttata ataaagacta tacggaggat gattaccatg acaaataaaa
      601 acaatgaacg ttactctacc gaactgaaag cgacagccat caagaaaatg atgccacccg
      661 aaaatagggg gattcgccaa ttaagtcaaa ctttgggcat tccagaagcg actctttatt
      721 cttggcgtaa aaaagctaga attcaaggga atactacacc cggaacgaaa aaaaatgtcg
      781 aaaaatggtc aagtgaagat aaatttttaa ttgtgatgga atcattctca atgaaccaag
      841 tagaacttgc ggaatattgc cgaaagaagg gcttatttaa agaacaaatt gaggcttgga
      901 agaaaacgtg cttatctgcg aatgaacagg aagaaaatcg aacgagagaa cttgcaactg
      961 aactaaaaga agagaaaaaa caagcgcgtc aattagagaa agaattacgt cataaggaaa
     1021 aagcattagc ggaagccgct gctctcttat tactaagaaa aaaagcacac gcgatttggg
     1081 gggaccaaga ggaagaatga tttcatcttc agatcgcaaa caagctgttg aacttattca
     1141 agaagccaat cgcaatgggg cccgtctcac ctatgcctgt aatgagctga atattagcgt
     1201 tcgaacctac gaacgatgga cgcgtgaagg tacgatagcg catgatcaac gccctttggc
     1261 gaaacgtcct gtacccaaaa ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac
     1321 cgtttcaaaa aaagaattta tgaatttgcc acctagccaa attgtaccaa aacttgcgga
     1381 ttgctcgatt tatattgcat cagagtctag cttctatcgt gtattgcgag aaaaaaatat
     1441 gcaacatcac cgtggccgaa gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc
     1501 gacgaagcca aatgaagttt ggacttggga tattacttgg ctaaaaggtc caataaaagg
     1561 actcttttac cggttatatc tcatcattga tttatttagc cgtaaaatgg taggctggga
     1621 gatttgggaa tcagaagaag ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa
     1681 ggaacgtatc caaggacagc cgttggtttt acactccgat aatggcagcc ctatgaaagc
     1741 cgcgactttt caagtcttac tcgaaaaatt aggaattcaa agttcttatt caagaccacg
     1801 agtgagtaat gacaatcctt attcggaagc ggctttccga acgttaaaat atcgaccaga
     1861 atatcccaat aacggcttta aaacaataga tgatgctcga aaatggacga aagagtttgt
     1921 cgattggtat aacgattctc atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg
     1981 tcatgaagga cgacatctcg ctattttaga gaaacgaaag caggtttacg aggacgctaa
     2041 aaagcgccat cctgaacgct ggtcaaaagg tgttcgaaat tggactccac atcaatcggt
     2101 ggcgttgaat cctttaaaag aaacagaaag ttaacaggag cataatcttt caagattatc
     2161 aaataaccct cgtcacccaa taggggaaaa agttattctt cccctattgg ataacgaggc
     2221 caaccgacca tagggaggtt ctgctttttg ataggtggaa aataaaaata cgacaactat
     2281 attgacaaac ac
//
LOCUS       DASJST010000242         2277 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJST010000242 DASJST010000000
VERSION     DASJST010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.248"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..1066)
                     /locus_tag="U4B05_002888"
     CDS             complement(32..1066)
                     /locus_tag="U4B05_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN2051971.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(1070..2128)
                     /locus_tag="U4B05_002889"
     CDS             complement(1070..2128)
                     /locus_tag="U4B05_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN2051972.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT          713 a          478 c          381 g          705 t
ORIGIN      
        1 aaacatttta ttgacgaata tccatgtttt cttatcttaa ttgacgggct ttaggaagag
       61 atacctcatt tttcgttttt ccagtttcta ttatttcacg aaagttctcg aaacttgtcg
      121 aaatcatatt tgataatgcc tcatctgtat ttgaacctac atgcggggtt acaagaactc
      181 tagggtacat ttctactaat tgctggatag cagggtctgg aatcgtctcc catggcttga
      241 aggatcggaa aaaaacctct gtttcatttg caaaaacatc ggttgcaaaa ccagctaaat
      301 gattgctttt caaagctctt aaaatagctt cattatcttg aagctctcca cgggctgtat
      361 taatcaagat gctacctttt ttcatttgag ctaaaaatcc ttcattgatc atttgatcgt
      421 tttcacctgg gatatgaggt acatgaatac tgacaatatc gcttttggct aataactctt
      481 caagcgagtg gaacgtcaat acttttttag cagcttctgt ctgatgaata tcgtagccga
      541 tcactttggc tcctagtccc ttaaacaaaa cagcttccgt caaccctatc ctgcctgttc
      601 ctatgatccc tactgtgcaa tttctgattt cacgactaaa catttgctca tccactcgaa
      661 aatctttgaa agatgttcgg atcgtcgtat acgcagtatt tctcaaaagc atcatggcaa
      721 aagtcaaaga taactctgct atcgcatttg gcgaatacga tggtacacgg gctactgtca
      781 taccaaaatc agctgccgct tgtagatcga tatgattgac tcccacagtc cgtgtaaaaa
      841 cataacgaat cccatattcc tgcatttttg caagattggt ctcatctgct atacaatttc
      901 ccctcaacaa tacagcatca tggtcctttg ctgacacgat attgtcatgt gtcaacagtt
      961 cttcataaag actgagatca taatggtaaa tattcaattt ttcaaaaaaa tctatctcgt
     1021 tcggacgaac accgtaacat gcgattctca tactttcttc ctccatttct tatgaaaata
     1081 aacctatgcc atgaatcatt tgtaatacaa cgatccatac aggtgtcagt accacagcaa
     1141 aaatagttga cagtaaggat gcattggagg ctaaaagtgc ctccctatca aaactgatag
     1201 cataggctgc tgccactgtt gcagctggtg tagccatcat gataacgatt gttgccagtg
     1261 cttcaaaatt gactggtaga atattcgttg ccgtcaaaac tgctagaatc acgatattca
     1321 atgcaggtac aaataccact ttgactagac tgtaatacca tgaagttttt tcagatgcag
     1381 cttttttgaa gcttacttca cctaatgttg cgccgattgc tagccacgcc aatggtgaac
     1441 ttaaaccagc taaataatcc atcggctgaa atagccacgg tgcagtctgg tcaattcgca
     1501 aaaacgctac ttggcttatc tctccttctg cacctgtaac tgacacttgt ggcagatacc
     1561 cttggaacag ccaaatgaaa aggccggcaa aagtagcgat aatgattgga ttgacgaaca
     1621 tcgctttgat atttttagct tccatcttca agccactcat ttttatgtat ccataagaat
     1681 acaaaaagat tcggtagcca atattgaaaa ttgaggaata catcacgccg acaggaccat
     1741 aaatcgcact gacgataggc gttccgaaaa aggtcgtaga accaaagatc gtcaggacac
     1801 gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa aggttttgaa actagaataa
     1861 gaataatata aataactaat ccccaaatca agacattgat tccttgcatc agtaattctg
     1921 tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg caaagccact gttaagacga
     1981 ctttcgatag aatttttcca acagattcag aaaaaatccc cttttttcta caaacaaacc
     2041 ctaataatat aataaaaact gttgaagtga ttgcactgac aatcgacata tctgtcaaag
     2101 tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct aattatgttc atttattcac
     2161 aataaaaaaa gcttttattt tgctaatacg tattaacatt tttaatttta tctttacatt
     2221 gttacttatt gaacaatagg aaattaaaga aacttttgaa acttatgtaa agtgatt
//
LOCUS       DASJST010000243         2266 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJST010000243 DASJST010000000
VERSION     DASJST010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.249"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            381..618
                     /locus_tag="U4B05_002890"
                     /pseudo
     CDS             381..618
                     /locus_tag="U4B05_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(762..1538)
                     /locus_tag="U4B05_002891"
     CDS             complement(762..1538)
                     /locus_tag="U4B05_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2051973.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            1661..2068
                     /locus_tag="U4B05_002892"
     CDS             1661..2068
                     /locus_tag="U4B05_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEN2051974.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT          773 a          366 c          346 g          781 t
ORIGIN      
        1 aaacaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata ccgaaggtca
       61 gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa
      121 gtaacaattt caaaaaacag cttctcatct ttgatgaata tcgtgacttg tggacgaggc
      181 gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac
      241 gttgttttga ttgcccttaa tgaggacagt cggaacaggt ttgtcatttt tatatttaaa
      301 tggcacaaat ttttctaatt atttgtcatt tagcgctgaa aatttttgct atttatctaa
      361 aaaagtgata actacttaag atgaagcaga atatttacgc catacggcat acaaccgcga
      421 aaaatataaa aaaaagaaaa cgattgaacg cgtttttgca gtcgcaaagg aaaagcatgg
      481 aatgcgctac atcaagtatc gagacttgga aaacaatcag gtgtatacgc tgattgtttt
      541 tgctgcaatg aatctgaaaa agttgtccac ttggcagttg gaacatatgt cttcatatct
      601 tttattatac aaaaatagca aaagcaccag tctacactct tagtaaagag gaaactgatc
      661 cttttgtcta cattcttaaa gtacttaaac taagtacttt attttactct ttaggattga
      721 tacagtagca tctatccctt tattaataat cttttatttt tttaaataaa gatgttgtct
      781 ccatcttcag gcactaaaac tttttcggaa attccttttt ctttattgaa agactttaat
      841 tcttctcttg acagtcccca atggtttaca gcttccatat gaacggcaat aattgtggaa
      901 tctggtattg ctttatgcaa ttcatataaa tcgtctttcc ccataataag agattctcca
      961 actaaaaaat gattatctcc agcatttgct actatgattt ctggattata ctgatcaata
     1021 gtttgtttaa cagcttcata ccaaactgta tctcctgtaa agtatagtgt cttctcagtc
     1081 gaatgtttga aaacaatacc gcaaacatta cctacataat tcaagatttc tcctctgcca
     1141 tgtttgcctt ttgttttaac caattgtata gattcaaaaa ctgtgtcttc tcgtagtacc
     1201 tccacatttt taaaaccata tagtcgaatt tcattcgcat cattttcatc ttgcacaaat
     1261 aattttattt ctttaggtag gacctctttc gctatatcgt cccaatgatc tagatgtaaa
     1321 tgtgttacaa taacagcatc aataccgttg ataatatctt cgactggcat tggcaaatca
     1381 actagaggat tcatttgatc ttttcttggt gatggaaaag gacctttcga aataaatggt
     1441 ggaagtgttc ctttttccgc taacattgga tcaattaaaa attttttacc tccatagtct
     1501 aagatagctg ttgcattacg aatgtgatgt atattcataa acgttgtctc ctttttgttg
     1561 atatgaaata tttttagtat aattcaattc catacaataa ataaaccgaa caaaaaaagt
     1621 cttttactat ttttacttaa tttatgaaag gttgtttacc atgttagacc aaacagatat
     1681 aaatatcatt gaggaactat caaaaaatag tcgaatcaca atgaaagaac ttggagaaaa
     1741 agtacatttg actggacccg ctgtctcggc gagagtgaca aaactagagg aaagtggagt
     1801 tattgaaaat tatacagtaa aattaaatat ggaaaagcta gggttttcga ttcacgcttt
     1861 tttaattata attacgcaaa atttaaatca taaaccctat ttatcattta ttaaaaatca
     1921 tgaaaaattc ttgattagaa attataagat tagtggaggg ggctgttatc ttttggaatg
     1981 tagatttact tcaaaaaaat caatgaacag ctttttggaa cagcttaatg aatatgcgaa
     2041 ttataaatta tctattgtta tagattaata ataaaactag cagatggtaa aaatactaat
     2101 atgcgagaca cacattcaac cataactata taacttatca atttaaaact aaactgatgg
     2161 aaacaagtaa ggcaaaaaca tttatatttt gccctactta tttttttaat taaattcatt
     2221 gatgattcat atttagaaaa ttctatgaag gccaaatgag tatcct
//
LOCUS       DASJST010000244         2197 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.250, whole genome shotgun sequence.
ACCESSION   DASJST010000244 DASJST010000000
VERSION     DASJST010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.250"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..750
                     /locus_tag="U4B05_002893"
     CDS             <1..750
                     /locus_tag="U4B05_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2051975.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          788 a          292 c          446 g          671 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaacg
      721 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
      781 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      841 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      901 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      961 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
     1021 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
     1081 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
     1141 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
     1201 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
     1261 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
     1321 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
     1381 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
     1441 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
     1501 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
     1561 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
     1621 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
     1681 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1741 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1801 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1861 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1921 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1981 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     2041 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     2101 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     2161 attcaatttg aattaagcaa aaaaaggctg agtttta
//
LOCUS       DASJST010000245         2181 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJST010000245 DASJST010000000
VERSION     DASJST010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.251"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            32..160
                     /locus_tag="U4B05_002894"
                     /pseudo
     CDS             32..160
                     /locus_tag="U4B05_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            274..513
                     /locus_tag="U4B05_002895"
     CDS             274..513
                     /locus_tag="U4B05_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051976.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            625..819
                     /locus_tag="U4B05_002896"
     CDS             625..819
                     /locus_tag="U4B05_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051977.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            905..1576
                     /locus_tag="U4B05_002897"
     CDS             905..1576
                     /locus_tag="U4B05_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN2051978.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1629..>2181
                     /locus_tag="U4B05_002898"
     CDS             1629..>2181
                     /locus_tag="U4B05_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2051979.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMV"
BASE COUNT          803 a          296 c          488 g          594 t
ORIGIN      
        1 cgtaagaaac cgttaggttt agctaagtgg tgagtagttc acaatgacgc ttacgaatga
       61 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
      121 cgttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
      181 acagataaag gcttagaaga agtcacgacg aataataaaa aggtagtatt accttaggca
      241 ttgagtaagg aattcattta ggaggaaaat tagatggcgc gaccagtttt agaggcgatg
      301 gatatggata ctttgacaaa aggaaggtat gtacagctgt tagctcgtgg ttattcacct
      361 aaagatgtat tcaaatccct gtctaaagga acagatatgg aaaaagaaag attacgaaaa
      421 gaattcgatt attggagaac ccataatgga atcaaggatt tgaaaccagc acggccagta
      481 attaaacgaa agaaaaagat aacccaagac tgaagtgtag agcgtatgtg atcataggct
      541 tttttatttt tgcaaaaaac aaggaaggta ggaaaactca tgaattatga agaaatcgaa
      601 aacagaaaaa aagtgtcgaa ataaatggaa gaaaagttgc taaaaacgat gaaacaaaaa
      661 caccttaaaa gattatctgt catgcaatat atcaatgata tgcagataac aggaaaagag
      721 aaagcttgtt tgttaggttc aatgaaaaac tttgaacagt tgagacgaac ttatgtgaaa
      781 acaagttcta attgtcaact attgttagaa gtctcatgac attcattaat agaattcatt
      841 tttaaaactt tttctatgga aatgagaaaa agattttttg ttttgtctga aagggggtag
      901 ctggatgaga aaatggttat ttggtttttt aggagtcgct ttaatcgtct tttgcagtgt
      961 gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa atatagaaaa
     1021 aaagatgccc gtagatcaaa tcaatgcaca tgcaaagagt tataaggagg atgcaacgaa
     1081 cgtcaataat gatatgtctt tggggcaaat gttgtccata caacaagaag caatcgaaat
     1141 gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt tagcattgcc
     1201 aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt atttttatga
     1261 agatgctgaa atgggcaaag gaaactatgt cctagcaggt cataacatgg aaatgccagg
     1321 tgttttattt tctgatattc aaaagttgtc attaggggag gtgatggact tagttagtaa
     1381 cgatggcgtc tatcgttaca aggtaacgcg taaatttatc gtacctgagt actttaaatt
     1441 aatcgatggc gttcctgaag agaactcttt cttgtctttg ccaaagaaag gagagaaacc
     1501 cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat acgtggtaca
     1561 gggagaacta caataacaag gttttaatca acgtgagtcg ttgaaatatg aaatgaggag
     1621 agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgcttatt ggaatgttag
     1681 gaatcattgc aggctgcttt cagggatatc gagcatatgc agaagatgtc acgcaaaaaa
     1741 cgccaccaga gaaggtcaat atcacggttc ataaattaat gtacgatcaa ggaacgcaat
     1801 tgaatgttga tattgacggg atcaaaaacg atggttatac gcacgatcaa tatcctacag
     1861 gtgtaacgaa atacaacaag gcagattatg gtgatgttga gtttacgtta ggaaatatta
     1921 cggatcaagt attaccgaga gaagattctg atctaaccaa cgcgaaagtg gacgagattg
     1981 tacaagatgt agaagacaag ggatctaaca gtgagtacgt caagaatgca accaacaaaa
     2041 ttacttcagc tgtagatgaa aatggtgaga tcacttttac tgatcaacca gcctatgtca
     2101 attccaaagg gaatgtatat gtggtttatg aaagtaaatc cgctgctgga ttagtcacac
     2161 aaaaagcaaa accaatggtt g
//
LOCUS       DASJST010000246         2161 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJST010000246 DASJST010000000
VERSION     DASJST010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.252"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(68..988)
                     /locus_tag="U4B05_002899"
     CDS             complement(68..988)
                     /locus_tag="U4B05_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN2051980.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            1153..>2161
                     /locus_tag="U4B05_002900"
     CDS             1153..>2161
                     /locus_tag="U4B05_002900"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN2051981.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VV"
BASE COUNT          700 a          406 c          449 g          606 t
ORIGIN      
        1 gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt tgcatcgttt
       61 gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca gaagctgtca
      121 attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt ccgttcaatg
      181 cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat ccaaagatag
      241 aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt actcgatcaa
      301 tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct aaaagtactt
      361 gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg atgaaatgga
      421 cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg acggttcctt
      481 caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt tccatgataa
      541 taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg attgccacac
      601 ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca gataaaattg
      661 catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga ctatgtaaag
      721 tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa gacttatcaa
      781 tcagatattt tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa atcagatata
      841 aacttttttg gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc caaaaccgct
      901 gaaacgcatt tactttgctg acagcttgat ctgtcaatgt ctcgccagaa gagtctgtcg
      961 taccggcagt tgctaataat gaaaacataa aatcctcttt tctaaaattt gattctttct
     1021 gtattgtacc aagaagtttt caaaaaagga aatagcaaaa ataactataa tgaaggaaaa
     1081 aagctgaaaa taaaatgaca aataatatgg ataatgataa aatgtggaga aaataacgaa
     1141 ggtggtaagt atatgcaaat cgattggaac aatctaggtt tttcttatat caaaactcct
     1201 tggcgtttta ttgccaaatg gaaagacgga caatgggaag aaggagaatt gacagaagat
     1261 aattacctga cgattcatga aggctctcca gcgcttcatt atggtcagca atgttttgaa
     1321 ggtctaaaag cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc tgaacaaaat
     1381 agtcgacgga tgaatcaaag tgctagacga ttgctgatgc caatggtccc tgaaaagatg
     1441 ttcgttgatg ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc gccctatgga
     1501 acaggcgcta cattatactt gcgcccttta ctgattgggg tcggtgaaaa cattggtgtc
     1561 catccagctc cagaatattt atttgtcgtc ttctgtacgc cagtaggagc gtatttcaaa
     1621 ggtggactaa aaccaacgaa ttttattgta tctgattacg atcgtgctgc tcctaagggg
     1681 actggtgcag caaaagtcgg cggaaattac gcagcgagtt tacttccagg aacagaagca
     1741 aaagaactgg attactcaga ttgtgtttat cttgatccag ccacacatac gaaaatcgaa
     1801 gaagtaggtg cagcaaattt ctttggaatt acaaaagacg gaaaattcat cactccgcaa
     1861 tcgccctcta tcttaccaag tatcacgaaa tattccttgt tgcagttagc tgaagaacga
     1921 ctaggactgc aagcaattga aggagatatt ttcattgatg atctagatca atttgcagaa
     1981 gctggtgcat gtggtacagc ggctgttatt tctccaatcg gtgggattta tcacaataac
     2041 aatcttcatg tattttatag tgaaacggaa gtaggaccag taacaaaacg gttgtatgat
     2101 gaattgacag gtattcaatt tggagacata gaagcaccag aaggctggat tgaagtcgtg
     2161 g
//
LOCUS       DASJST010000247         2141 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJST010000247 DASJST010000000
VERSION     DASJST010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.253"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1735..>2141)
                     /locus_tag="U4B05_002901"
     CDS             complement(1735..>2141)
                     /locus_tag="U4B05_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN2051982.1"
                     /translation="EVDRTPAENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQ
                     LGELYKWFTNTYAHLPLKELEEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKT
                     AVWEVYKFIHVNTVAPFGTFRTKIRKWKRLAL"
BASE COUNT          721 a          383 c          315 g          722 t
ORIGIN      
        1 cagagaagtg gggcactcag tagtattaac aatttaaaag gaatttctta aagaaaatgg
       61 gttgcatcct ggagatacaa tttttttaga taaagaaaaa attcaagcaa caattcaaaa
      121 aaatcttaac tgctgagatt gatgtagcaa tatctcaatc atcaacaaag tacgatcctg
      181 cctttcaaaa gcttattgat aaatgtttat tcattataaa aataaaagaa aaatttatgt
      241 cagctttttt catcaaagtg gtaatgataa ttcgtctatt aattgtattt tttaggaaat
      301 atggtataat tgttttaacg aaaaggtaat tcatgataca ccaaaaacca ctagctggcg
      361 ggctagtggt tttcttttac gttctattca gattaacggg taggcttact gtcttttttg
      421 gcaataattt tgcaatcaag ccatttacag atgtaatgcg cggatacttc tgctaagatt
      481 gcaaaaaaca tatcgaaaag gtaattcata atacaacctc ctccccttgc cataataggc
      541 cggggtagca gacagcataa atattatacc ataactgaaa atttcatttt agtacatttc
      601 aatatattga tgaaccagca caataaaaca tgccatgact aacagaaaaa aacttattaa
      661 accagatata acttgtaaac atccaactat atagaaaacg taaattttct atatagttgg
      721 attaagataa cgactattta ttttatcata tttttactat accctatact tttacttggt
      781 atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt agattaataa
      841 aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa ttgatggtat
      901 ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga cgattgctac
      961 tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga aatgaaagac
     1021 cttttacatc ttgcagaaat cgttactggg attaaactaa gcaataaaaa ctaatgttcc
     1081 cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac gatttcacct
     1141 tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc agtaatagga
     1201 taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat ctgtatcgta
     1261 tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa gcgttgaatc
     1321 gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta ccggacttgc
     1381 cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg gtactatcgg
     1441 cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc ctaagtccct
     1501 ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt ttatctgttg
     1561 gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag gggagtgcac
     1621 ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg tatgttctta
     1681 ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg gaatttacaa
     1741 agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag caactgtatt
     1801 tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt ctgcccattt
     1861 tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca tgttaatatc
     1921 atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg cataggtatt
     1981 tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag aaggcgtttt
     2041 aacagataat ccatttcttt catcattttc ccattttaga attaaattcg tccaaccaac
     2101 ttgatatgct aaattttcag caggtgttct atccacttcc t
//
LOCUS       DASJST010000248         2113 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJST010000248 DASJST010000000
VERSION     DASJST010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.254"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            316..933
                     /locus_tag="U4B05_002902"
     CDS             316..933
                     /locus_tag="U4B05_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2051983.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            974..1273
                     /locus_tag="U4B05_002903"
     CDS             974..1273
                     /locus_tag="U4B05_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN2051984.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1307..1474
                     /locus_tag="U4B05_002904"
     CDS             1307..1474
                     /locus_tag="U4B05_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2051985.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1519..>2113)
                     /locus_tag="U4B05_002905"
     CDS             complement(1519..>2113)
                     /locus_tag="U4B05_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2051986.1"
                     /translation="GYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERPEFQK
                     LLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          702 a          344 c          406 g          661 t
ORIGIN      
        1 aaaaattgta aaagttaaag aagtcaaatt ctgatgtgaa aaataaaata taccatggaa
       61 gacataaaag aaaaactgga taactagttt atttaatggc agaccgttaa ggcctgccat
      121 ttttatgtat tttagcggaa taaatataat catgcgggag tgtaaactta ctgtttcaaa
      181 aagtatactt tttgacatgc cttgattttc tttgtttatg tatggcataa taaccacata
      241 atacaatttg aaacgtttct gatttaatct gattgttttg aaacaatttt agtgaaaaaa
      301 gggtagataa aaacgatgag aaaatttttt tatatccggg tcagtagtta tgatcaatcg
      361 atcgaacgac aactgatttc cgcgaaggaa ttaggaatcc ctgaagaata tatatttgta
      421 gaaaaagcca gcggcaaaga ttttaaacgc cctgaatatc aattaatgaa acggatgttt
      481 cgaaaaggag atttactcta tctacaatca ttagatagac taggcagaaa taaacaaatg
      541 attttagaag aatggcgaga attaactaag gaaaagcaga ttgatatttc tgtgttagac
      601 atgcctctgt tagatactgc tagttacaag caactagatg ggttagagac gcttatctct
      661 gacttaattc tgcaattact cagctatatg gccgaagacg agcggaaacg aattaatagc
      721 cggcaaagag aaggcgtaga agctgcgaaa caaaagggtg taaaattcgg cagaaagaaa
      781 attgctttac ctcctgaatt tcctgcgatc tattctgact ggaaagcagg aaagataact
      841 gcagtctatg ctatggatca attgggcatg aaacgaaata ccttttatcg acgggtgaaa
      901 gaatatgaac ataccttgca ccacttttct tgatagatat aacagctatt agacgggaag
      961 ggagagtttg aaaatggact taacgaatca aagggtcgtt tttactggaa cgctgcagca
     1021 aatgacacga acccaagcca tcggtttggt tcactcttta aacggccatt gtcaatcaag
     1081 tgttacttca aaaacaaatt tcctggtttg tggtcagtat tctaagtcgt tgtttgatga
     1141 ttctctctat acaaaaaagg aacaaaccgc gcgggacttg attgcgttag gtcatgagat
     1201 taccatttta tcagaagtcg aattttatgc gttaattagt caacagttga aagaatggca
     1261 acggtattta taatcgttag taaaatttac aaggaggaaa atttttatga agcgaaaatc
     1321 ttatgatcgt tggggcattg aaataactag ttttaatcga aatattgctt ttcacgtgga
     1381 gtgtgactgt ggggaattag caaaaagtgt ttatcaaaaa aattactttt actgtcctaa
     1441 atgccatcgc cgatacgggg tacatggaac atagaaacga aatagtgcgg tagaaaagcg
     1501 aatgaatgta gtatatttct acaaatcatt cgctttcttt attttataaa tagttggtcg
     1561 agagatcccg ttttcttttg caatctctgc aactggaatg ccttgcttta acatgtccac
     1621 cacggtttta aaaactaagc gtttttgcgg atctgttgag ttaatagaat actcgattgg
     1681 tcgtccctta tatcggccgg ctttttgagc caataatact ccttgccgct gacgttcacg
     1741 aattttctca cgctcgtttt gtgcaatata acttagcagt gataaaaaca tatcgaacat
     1801 agcggtatcc aggagttcat ttccagtatt aaaatctaaa aactgtgcat ctaatatttt
     1861 taacgctact tttttctgtt tcataaaagc aactgtattt tttatatctt cataatctct
     1921 tcctaaacga tctagtgatg taacgatgac gcaatcacct tcacggatat aatttagtaa
     1981 tttttgaaac tcaggacgct cagtatcttt accactggct ttttcttgga ataatttatc
     2041 aatgttaagt ccttccatca ttgccaattg tcgatccaaa ttttgatctt gtgaagatac
     2101 tcgaatatag cca
//
LOCUS       DASJST010000249         2112 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJST010000249 DASJST010000000
VERSION     DASJST010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.255"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(520..1134)
                     /locus_tag="U4B05_002906"
     CDS             complement(520..1134)
                     /locus_tag="U4B05_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2051987.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1740..>2112)
                     /locus_tag="U4B05_002907"
     CDS             complement(1740..>2112)
                     /locus_tag="U4B05_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051988.1"
                     /translation="KLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRKKE
                     TFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRKGR
                     HTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          710 a          404 c          319 g          679 t
ORIGIN      
        1 ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca cttataaata
       61 tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag gtatcctgtt
      121 ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat gtactataat
      181 tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga gacaagaaag
      241 aatccatatg gattctttct tgtctctttt tccactactt tttatttttc tgacactaca
      301 tattcttata actcatttct cataactgtg aaacaactta taaaaataac aaactttaaa
      361 caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa aaaaagtagt
      421 cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt tttatgtgga
      481 acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac ttgtgtcctg
      541 gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga
      601 ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat catgtaaatt
      661 ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg
      721 cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat gaccattttc
      781 tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg aaagaagaag
      841 aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg caccttctag
      901 cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa gatccggggt
      961 ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata tctgcaatct
     1021 accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca gcggctcttc
     1081 tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac tcatttatct
     1141 ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag gaagtacata
     1201 aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca caaaaaaggc
     1261 attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca gaccatggaa
     1321 aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt aggtcgatgc
     1381 actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg gtcattccct
     1441 gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt tctatctgaa
     1501 tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa acattggtat
     1561 gatttatata tatataaata acataaaaaa aggaatggct tatcccgata caacaagcat
     1621 tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca attaaataaa
     1681 tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc tactaaatat
     1741 taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga aaaagtagtt
     1801 gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat ataaatgatt
     1861 agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc taacgccaat
     1921 tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat cagaaacgtt
     1981 tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag tggcaacaca
     2041 aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga ctgtgcaaca
     2101 gcgactaatt tt
//
LOCUS       DASJST010000250         2095 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJST010000250 DASJST010000000
VERSION     DASJST010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.256"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>2095
                     /gene="acm"
                     /locus_tag="U4B05_002908"
     CDS             <1..>2095
                     /gene="acm"
                     /locus_tag="U4B05_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN2051989.1"
                     /translation="ILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDG
                     GNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKD
                     SAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPAS
                     GSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEI
                     NQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKT
                     IIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYI
                     NDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNW
                     IDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFR
                     ASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGY
                     QKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEK
                     DDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHI
                     TPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILP
                     KTSSKKSSFAVIIGLL"
BASE COUNT          820 a          343 c          406 g          526 t
ORIGIN      
        1 attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt tcatgtctat
       61 gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat
      121 attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc
      181 caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg cggtgaagga
      241 ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt aacggtcaga
      301 aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc agtagaaggt
      361 tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga aaatacggga
      421 ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa gccagcttct
      481 ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga agatactaag
      541 cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca accagttaaa
      601 atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga aattaatcaa
      661 atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca atttttacaa
      721 gatttcccca gcgctacatt taatttttca gtaactgata attacattga aataactata
      781 ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat catagaaaac
      841 ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt taataaacct
      901 gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc tggtggagtc
      961 aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac agagattggt
     1021 atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca aggacagtct
     1081 agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct gcaagtagga
     1141 gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc actaagttca
     1201 aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt acctatttat
     1261 aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg aaccactcca
     1321 agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga accagttcct
     1381 gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga agatatccaa
     1441 cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga
     1501 aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag
     1561 aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga gaaccaagac
     1621 ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc gatccaacac
     1681 aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc aaaatacaga
     1741 gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata cagtacgacg
     1801 attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat atctatacca
     1861 ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga gaaaattaga
     1921 aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag cgaacaaaga
     1981 agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc aaaaagcatt
     2041 cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt attga
//
LOCUS       DASJST010000251         2088 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJST010000251 DASJST010000000
VERSION     DASJST010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.257"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(124..411)
                     /locus_tag="U4B05_002909"
     CDS             complement(124..411)
                     /locus_tag="U4B05_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051990.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(582..1934)
                     /locus_tag="U4B05_002910"
     CDS             complement(582..1934)
                     /locus_tag="U4B05_002910"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN2051991.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          570 a          418 c          359 g          741 t
ORIGIN      
        1 ggggttgaca aagcttgtca taaccccttt ttctgattaa aagatagtaa agagacagtc
       61 atcggtaatt gtgatgattt tttgtcatca cttaaggatg agcggccttt tcaaaaactg
      121 tctttaaaga tcatcatcag tttctagcac atatggctga ccttcatatg tgtactctgt
      181 attcaatgtg ccgtcatttt catctgagta gatgcgtcta ctgaaaggaa aacgatgaga
      241 aacattatct ttggcgtcat ttgctggcat cgtgtaatat tcctcttgcg tttcattcaa
      301 ctgagagagt acttgatctg ccaactcatc aatattgatc gttaatttct tcgaagcact
      361 tttataatct tctaaaaatg catcttgttg gacaaaatat tctttattca tcttctttct
      421 cacctttctt ttcaaaatca aataaagctg cttgttcttt tatgtaacag ctttatcttt
      481 gctgcttatt gtgacaaaaa attcgaagaa agacaactaa ttgtactttt cacaaaaaaa
      541 gacagagggt cgtgacaaaa ttgtgtcaca accctttttc tttattgtgc agtttcgatt
      601 tcgcctagcg ctgcttgtgc aagtaggttc aagtaattcc atgggcggtc aaaatgtggt
      661 tggaagaaga aatctgaaag tgcgagatct tctacagtca ttttattttg aacagctaaa
      721 gacatggtat ttgctgattg tgtaatgtcg tatttggaca tgaactgagc acctacgata
      781 cgattcgttc ctttttcata gactagctcc atcattactt tttcagtagt tggcatgaac
      841 tctggacgat aattatcttc aaatacagtc gcttggactt ctagatcgtt caaagttgcg
      901 ctttctttgg tcacacctgt cgaaccgatc gtccagccaa ataggtaaag tcctgaagtt
      961 ccttgagtac cacgatacgc cattttatga tctgtcaagt tatttcccac aagcaaacct
     1021 tgacgaacgg catttgttgc caaaggaata tagttttgcg tgccacttgg attgtagttg
     1081 actactgcag agtctccagc tgcaaaaatg tctggattac ttgtctgcat gtattcattc
     1141 actttgattg ccccgtttgg cagtgtatct acttttcctt cgatcagttt tgtatttgga
     1201 cggaagccca cacataaaat gaccatatct gtatcaaatg tatctgttgg tgtagctact
     1261 tgtttcactt ttccttcttc atctgtgata aattcagtaa cattttcgcc tagcgcaaga
     1321 gtaacaccgc ggtctgtcaa ttctttttct aagatatctg tgaatggttt atctagatat
     1381 ttattcaaga tgcgatctaa tccatcaatc aatgtcactt gttttccaga ttcgacaaaa
     1441 gcttctacta attcaatacc gatatatcca ccaccaacaa ttactacacg ttttgcttct
     1501 ttcgcttgtg caataatttc atttgcttga ttgtagtttt tgcataggag tacatttttc
     1561 gcatcgattc cactaatcgg tgggataata ggccatgaac cagttgtcat cactaattta
     1621 tcataagaaa ccgtttctgt ttctcctgtt ttcagatctt ttgccgttac tgttttattt
     1681 tctgtatcga cgtctgttac atcgtgttcc attttaactg ttgctcctaa tgaagataat
     1741 tcttcaggat ttgagtagaa caagcctgct gggtccttta ctactccgcc gacataaagt
     1801 gcgataccgc aagataagaa agaaatattg tcatttcttt catatactgt cacttctgct
     1861 tctggatgat tttttaaaat actttttaca gctgcagtac ctgcatgagt acagccaatt
     1921 actactactt tcatttatgg tccctccgat taacattttg tccttcgaca aaactactat
     1981 aacagaaata aaacggatac aacagtttaa tgcttgtgaa ttcacaaagg tttgtttgta
     2041 gtttcattcc ttttcaagca taagaaccca atatctacaa gattctta
//
LOCUS       DASJST010000252         2056 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.258, whole genome shotgun sequence.
ACCESSION   DASJST010000252 DASJST010000000
VERSION     DASJST010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.258"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            493..714
                     /locus_tag="U4B05_002911"
     CDS             493..714
                     /locus_tag="U4B05_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051992.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="U4B05_002912"
     CDS             715..972
                     /locus_tag="U4B05_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2051993.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="U4B05_002913"
     CDS             complement(1108..1710)
                     /locus_tag="U4B05_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2051994.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="U4B05_002914"
     CDS             complement(1777..1932)
                     /locus_tag="U4B05_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2051995.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DASJST010000253         2027 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJST010000253 DASJST010000000
VERSION     DASJST010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.259"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            62..1237
                     /locus_tag="U4B05_002915"
     CDS             62..1237
                     /locus_tag="U4B05_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2051996.1"
                     /translation="MTEKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGST
                     ETQQLLQTLKKEKEQLIDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEE
                     FNRSIEQLYQNDFVLVNLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMD
                     NSGFPSRLVVSNNNEIKNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLAL
                     TGYNGVLGYRTSKSQYGDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASL
                     EDVKKDNELWQQEVAPILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCN
                     VDASTPAWGQLGTDFYRNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1306..1905)
                     /locus_tag="U4B05_002916"
     CDS             complement(1306..1905)
                     /locus_tag="U4B05_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2051997.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          652 a          396 c          346 g          633 t
ORIGIN      
        1 taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa
       61 aataacagaa aaagcaaaag aaccgacaga tgataagcaa caactagtta ctcaagcaaa
      121 aaatctggct caaagctacg attatgacca agcgattgcg ttattggaaa aagatagtaa
      181 gaacggatct actgaaacac aacagcttct tcaaacacta aaaaaggaga aagaacaact
      241 gatcgactgg tctgatccta ctcagatctc tcatgtcttt ttccacagct tgatcgtgga
      301 cccgcaaaaa gccttccact ctcaacaggc acaaggatac aaagattata tggtgactgt
      361 tgaagaattc aaccgttcga tcgaacaact ttatcaaaat gattttgttt tagttaattt
      421 gaatgatttg atccaaaaag acgaacaagg aaatctttcc tttaacgggc ttgcgctacc
      481 tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc agctattatg aatatatgga
      541 taattccggt tttcctagcc gattggtcgt atcaaataac aatgaaatca aaaatgtcta
      601 taaagagaaa gataaagaag aaatcggtga ctttgacatg gttcctctga tcgatgcctt
      661 tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg
      721 ttacaacgga gttttaggtt atcgaacatc aaaatctcaa tatggagaca atgaaaaaac
      781 aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag acggctggag
      841 ctttgcttcc catacatggg gtcacttgaa tatgactcaa gcttcactag aagatgtaaa
      901 aaaagacaat gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa caaatattct
      961 gatttatcca tttggtgcag acatcagcga ctggcagcct tattcagcag aaaatcaaaa
     1021 atttgcttac ttgaagagtc aaggtttcga tattttctgt aatgtcgatg cttctacacc
     1081 agcttgggga caattaggta cggatttcta tcgaaatgct cggatcaata tcgatggcat
     1141 tcgttttcaa tctgatttag acggaaaaaa tcctattctt gatccattca tcaatgtcca
     1201 tgatgtctat gacaaagaag cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa
     1261 aaagccgaac tgacatttta tcgtcagttt cggctttttt atgctttatt tcttcttcgg
     1321 tgcgattccc ttgatcagga cttctgtcca ttctgctgta tatggaagca gttttgcgta
     1381 tatctccggc ttagaaattc cttctaccat tgcttgaaaa tagaggatca agaactcatc
     1441 cgaatatttc aaatccactt ttccttcttt ccgccctcga tggaaaagat caagcatgat
     1501 actgaaactt tcttgcgtat acgcatccat cgcgtcagaa aattcttttt tttctttttt
     1561 tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc actcgattga gataacggat
     1621 tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga agattttccg caatcaacgc
     1681 tttcgcttct tgccctaaat cattcataaa tgcaaaaaat acttcataaa tcagcccttc
     1741 cttgctgcca aaatatttaa aaatcgtcgc tttacttacc tgagcgttct cagctacctt
     1801 atccattgtc actccagcgg ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa
     1861 aatctctttc tttttctctt gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg
     1921 taattttaaa cttttataat atatatagtt caaatttatt ctgaggttta ttataactat
     1981 ctattgagaa agtatcaagc tagcaattcc ttgtttatga attcatt
//
LOCUS       DASJST010000254         2004 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJST010000254 DASJST010000000
VERSION     DASJST010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.260"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U4B05_002917"
     CDS             <1..378
                     /locus_tag="U4B05_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN2051998.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U4B05_002918"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U4B05_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN2051999.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2004
                     /locus_tag="U4B05_002919"
     CDS             1859..>2004
                     /locus_tag="U4B05_002919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052000.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFT"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DASJST010000255         1971 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJST010000255 DASJST010000000
VERSION     DASJST010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.261"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..425)
                     /locus_tag="U4B05_002920"
     CDS             complement(<1..425)
                     /locus_tag="U4B05_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN2052001.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="U4B05_002921"
     CDS             complement(534..1565)
                     /locus_tag="U4B05_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2052002.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          608 a          326 c          340 g          697 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga a
//
LOCUS       DASJST010000256         1952 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJST010000256 DASJST010000000
VERSION     DASJST010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.262"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            70..552
                     /locus_tag="U4B05_002922"
     CDS             70..552
                     /locus_tag="U4B05_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052003.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            749..1639
                     /locus_tag="U4B05_002923"
     CDS             749..1639
                     /locus_tag="U4B05_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN2052004.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          635 a          335 c          406 g          576 t
ORIGIN      
        1 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
       61 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
      121 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
      181 ggcctgatcc aaggaagcgg atttgtggtt tctaggaatt ttccagcttt gattgttttc
      241 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
      301 cagctgcttt acttttcttg cttattagta tttacatcta cgttttcttt gctgcctcgc
      361 ttgattatcg gccctaatgg tttgagcgag agtggattga aggatttagc ttattcctcc
      421 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
      481 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
      541 aataactatt gatggtggat catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt
      601 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
      661 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
      721 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
      781 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
      841 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
      901 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
      961 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     1021 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     1081 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     1141 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     1201 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     1261 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     1321 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     1381 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     1441 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     1501 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     1561 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     1621 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     1681 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     1741 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     1801 aaaaaagagc tacgacatag aactgtcgta actttttttg gctcttcgtc aaatcgtgtt
     1861 ggtgaagaca aatttgaata aaacttagac cgatatgcgg cgcgttctgt gcgccgcata
     1921 tttttttagt tttcaaagat cagaccttgt tg
//
LOCUS       DASJST010000257         1948 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJST010000257 DASJST010000000
VERSION     DASJST010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.263"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..1566
                     /locus_tag="U4B05_002924"
     CDS             100..1566
                     /locus_tag="U4B05_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2052005.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          605 a          334 c          398 g          611 t
ORIGIN      
        1 aaagaataca cacataaaat aacactgtct tgtaagtttg ttaaaaattg aatatgatta
       61 catctggata acaaattttt atcataaagg agagaacgaa tgaacaacaa acaattacgc
      121 tggtttactg ttgcgttaat tgcatttaac atggtttggg gcatggggaa tgtagttaat
      181 aactatgctc agcaaggagt atcagttgtc acctcttggc tattgatttt ggccatttat
      241 tttattccat acgcattgat cgttggacaa ttaggatcag ccttcaagga tagtaaagga
      301 ggcgtcagtt catgggtcga aaatacgaca tctaataaac gactggcata ctatgccgct
      361 tggacttact gggtcgtcca tattccttat ttggcacaaa agcctcaagc aattctgatt
      421 gctggtggtt gggcagtcca aggaaatgga aatatcgtaa atacgatgtc agtacaaatg
      481 gtagcagtta tttcacttat tatatttctt gcctttttat atttatcaac aaaaggactt
      541 tctactttga aagtgattgg cggactggcc ggaacagcga tgttcgtcat gtcgatctta
      601 tttattcttt tagctgtaac ggcaccgttc atcaatccaa cgatggaatt tgccacacca
      661 catatgaatc aagtgaaaac atatattcca acatttgatt tcagttattt cacaaccgtt
      721 tcgatgctag tatttgcagt tggaggggcg gaaaaaatat ccccttatgt caactctaca
      781 agaaatcctg caaaggaatt tcctaaaggg atgatcttct tagcagcaat ggtagggatt
      841 tgtgctgtat taggctcagt cgcaatgggt atgctgtttg ctagtgataa tattcctaaa
      901 gacttgatgg caaatggggc atacagtgct tttcaaattt taggtaatta ctatggtatc
      961 ggtaactcat tgatgatcat ttacgcattg acaaacatga ttggacaaat ctccgcatta
     1021 gccttttcaa ttgatgcacc actaaaaatc ttgttagcag atgcggatcc agaatttgta
     1081 ccaaactggc tacgtaaacg taatcgtaaa ggtacattgg tgaatggtta tcttctgaca
     1141 ggtatactag taggaatcat tattttgttg ccgatttttg gtatcaaaga tatgaatgaa
     1201 ttagtaaaat ggttgacgaa cttgaactca gttgtcatgc caatgcgtta cctatgggtg
     1261 ttcttggcat atatgatgtt gaataaagct tataagaaat tcaattctga atataaattt
     1321 gttaaaaatc caaaaatcgg ttttatcttt ggtgcttggt gctttgcctt cactgcgttt
     1381 gcatgtatcc taggaatggt gccaaaagta gattatactg ctgatcctaa agcatggtgg
     1441 ttccaattat ttacgaatat catcacacca atcgtcttga tcttattagg tatgatcttg
     1501 ccagcaattg ctcgtcgaga caaaaacaaa gaaatcactg aaccagaaac agtggttcag
     1561 ccttaaaata gaaatacttg ttacaagagg ttgtgacaga agtggtcagc ttcaagaaat
     1621 aagaaggaat ttacgaaaat tgcttctcaa atttttgtga agtttcggct tgtttttcga
     1681 agaagctact tctggaacac cgtgaatcag aggttgtgaa aaagctgtcc tgcatcaagt
     1741 aataagaaca gccaaacaaa aatagcccct catatttttc gtttggctgg gcttattacc
     1801 gcagatgcaa gctttggaac accgtttaag gaagaggttg taaaatcagc gatttgacct
     1861 gagtattaga gaaacaaacg gaaaaatagc tcctcatatt ttgccgcttt ttgagctaat
     1921 gctgaaggtc agctgattga acaccatt
//
LOCUS       DASJST010000258         1931 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJST010000258 DASJST010000000
VERSION     DASJST010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.264"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            468..1640
                     /locus_tag="U4B05_002925"
     CDS             468..1640
                     /locus_tag="U4B05_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HEN2052006.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1770..>1931)
                     /locus_tag="U4B05_002926"
     CDS             complement(1770..>1931)
                     /locus_tag="U4B05_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HEN2052007.1"
                     /translation="RMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRK
                     EIYKRTYKD"
BASE COUNT          671 a          357 c          306 g          597 t
ORIGIN      
        1 aaaaagttca aaagatcaag tgtgcgcttc gctcccactc ccctcgacgt tttagtagcc
       61 tttccctcac ttcgttcagt ccaagccaac taaaaagttt tcgggataca ttctccttct
      121 cttcctaata atcaattaaa attttaatct ttatatttct gctaatcact caggaaacag
      181 tacagaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg ggttttattt
      241 tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gtttttgggc gttctgctag
      301 cttgtttaaa gtctcttgaa tgaatgtatg ttctaagtca aaaaactctg acagcgcctt
      361 tatatagata aagtccgtat aattgtgtaa aagtaaaaag gccatataac agtcctttta
      421 cggtacaatg tttttaacga caaaaacata cccaggagga cttttacatg acccaagtac
      481 attttacact gaaaagcgaa gagattcaaa gcattattga atattctgta aaggatgacg
      541 tttctaaaaa tattttaaca acggtattta atcaactaat ggaaaatcaa cgaacagaat
      601 atattcaagc aaaagaatat gaacgaacag aaaaccgaca aagtcaacga aatggctatt
      661 atgagcgcag ctttacgaca cgtgtaggca cgctagaatt aaaagtaccc agaacacgtg
      721 atggccattt ttcacccaca gtgtttgaac gttatcaacg aaacgaaaaa gccctcatgg
      781 cttcaatgtt ggaaatgtat gtatcaggcg tttcaactcg taaagtatca aaaattgtgg
      841 aagaactttg tggtaaatcc gtctctaagt ccttcgtttc tagcttaaca gaacagctag
      901 aacctatggt taacgagtgg cagaatcgtt tattatcaga aaaaaattat ccttacttaa
      961 tgaccgatgt actctatata aaagtacgag aagaaaatcg agtactctca aaaagctgtc
     1021 atatagcgat tggaataacc aaagatggcg accgtgaaat tatcggcttc atgattcaaa
     1081 gtggcgaaag cgaagagacc tggacaacat tttttgaata cctaaaagaa cgcggtttac
     1141 aaggtacgga actcgttatt tctgatgcgc acaaaggatt agtctctgcc attagaaaat
     1201 ccttcaccaa cgtaagttgg caaagatgcc aagttcactt cctaagaaat atctttacca
     1261 ccattcctaa aaaaaattca aaatctttca gagaagctgt taaaggaatt tttaagttca
     1321 cagatattaa cttagcgcgt gaggctaaaa atcgattgat tcatgattat atcgatcaac
     1381 caaaatattc aaaagcttgc gcatcattgg atgatggatt cgaagacgcc tttcaatata
     1441 ccgtacaagg aaattcccac aatcgactaa agagtaccaa tctaattgaa cgactgaatc
     1501 aagaagtacg cagaagagaa aagattattc gcatcttccc caatcaaaca tcagccaatc
     1561 gcttaattgg agccgttctt atggacctac atgatgaatg gatttattct tcaagaaaat
     1621 acatcaattt tgataagtag aaatggtaaa aacattgtat agcattttac acaggagtct
     1681 ggacttgact cacttccttt attatttttc atttttttaa actatttaat actaatgtct
     1741 tttataatag cttttcatat attatataat caatctttat aagtcctttt ataaatttct
     1801 tttctaccat tttcgataaa ttcctgttta atatttttaa ttccataaac aatagtttca
     1861 ataggataat attcttcaac tatatcttga tattcttttg ctttctcaat atctatattt
     1921 ccatacattc t
//
LOCUS       DASJST010000259         1859 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJST010000259 DASJST010000000
VERSION     DASJST010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.266"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="U4B05_002927"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="U4B05_002927"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2052008.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DASJST010000260         1847 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJST010000260 DASJST010000000
VERSION     DASJST010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.267"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="U4B05_002928"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="U4B05_002928"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2052009.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DASJST010000261         1749 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.268, whole genome shotgun sequence.
ACCESSION   DASJST010000261 DASJST010000000
VERSION     DASJST010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.268"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..718
                     /locus_tag="U4B05_002929"
     CDS             <1..718
                     /locus_tag="U4B05_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2052010.1"
                     /translation="HAATKVHYELAKKCLENGIHVYIDKPLSVHLNEVRELQELADKN
                     QIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDLFLHLIDTAVY
                     LLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEKYQVTSKNGTY
                     EVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGKSETELLKQKE
                     VVRSHELCEEVLKDHYRHQL"
     gene            791..1726
                     /gene="murB"
                     /locus_tag="U4B05_002930"
     CDS             791..1726
                     /gene="murB"
                     /locus_tag="U4B05_002930"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN2052011.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
BASE COUNT          622 a          285 c          389 g          453 t
ORIGIN      
        1 ccacgcagca acaaaagtac attatgaatt agccaaaaaa tgtctagaaa atggtatcca
       61 tgtttatatt gacaaaccac tgagtgtcca tctaaatgaa gttcgtgaat tgcaagaatt
      121 agctgataag aaccaaataa ttttgatgat cggtttcaac cggcgctttg ctcctatggt
      181 agaagaattg aaacgtatcc cagataagcg gttgatccac ctgcaaaaaa atcgaatagc
      241 ggcaaaagaa acgacagagt tcgtccttta tgatttgttt ttacatctta ttgatacagc
      301 tgtgtattta ttagatgaac ccattttgag aacaaaatac cagataagag aaacaaagga
      361 atttttggaa tatgccattt tacaattaga aacacaaaat caaacggcta ttttaacgat
      421 ggatttagca agtggcgcta atatagaaaa atatcaagta acaagtaaaa atggtactta
      481 tgaagtaaac gatctgaccc agcttgtcat tcaagatgct gatggaaaaa aagtgaaaga
      541 atttggcgac tggacaaaca cgctagtcaa acgcgggttt gagccaatgg tagaggcctt
      601 cttttctcag atccgtttag gtaaatcgga aacagaattg cttaaacaaa aagaagttgt
      661 aagaagtcat gaattatgtg aggaagtgct taaagatcac taccgtcatc aactttgaac
      721 aagactggca agattgtatt aagcgtgata gaataatgag gacgtttgag aaaagaaagg
      781 aaaatcctca gtgaataaag aagaaatagt tgaaagctta gccacgatta ctatattgtt
      841 tgatgaacca ttgatgaact atacgtttac gaaaacaggt ggaccagctg atgtccttgc
      901 attcccgaaa aaacaagaag aagtaaagca aatcatcgat tattgccgaa tccatgatat
      961 accttggatg gtattaggaa atgcaagcaa tctgatcgtt caagatggcg ggattcgcgg
     1021 tgtagtcatc atgctgactg aaatgaaaca gattcatgtc aaaggaacga tggtcattgc
     1081 ggaagcaggt gcctcactga ttgatacgac ttatgcagct ttggcagaat cattgactgg
     1141 atttgaattt gcttgcggca tcccgggaag tgtcggtgga gcggtttata tgaatgcagg
     1201 tgcgtatggc ggagagatca aagatgtttt tgctgaagtg gatcttttgc ttgaagatgg
     1261 aacattgaaa actttgacaa aagacgaaat ggtattttct tatcgtcaca gtaaagtcca
     1321 agaattacgt gctatcgtat tggaagctcg attcagtttg caaacagggg attatgaagc
     1381 aatcaaagca agaatggatg agttgaccga attgcgtcaa tcaaaacagc cattagaata
     1441 cccttcatgc ggaagtgtct ttaagcgccc agtaggacac tatacaggtc agctgatcca
     1501 acaagcagga ttgcaaggcc tgaaatgggg cggtgcacaa gtctctgaaa aacatgctgg
     1561 attcatcgtc aatatcgatc atgcgacagc gacagattat attgaattga ttgcgcacat
     1621 ccaagaagta atcaaagaaa aatttgatgt gtctttagaa acagaagtgc ggatcattgg
     1681 agaaaaacaa gcaatatcag aaacaagcag aagtttagcc aattaataag taatggaact
     1741 taaaataag
//
LOCUS       DASJST010000262         1727 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.269, whole genome shotgun sequence.
ACCESSION   DASJST010000262 DASJST010000000
VERSION     DASJST010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.269"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..509
                     /locus_tag="U4B05_002931"
     CDS             <1..509
                     /locus_tag="U4B05_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052012.1"
                     /translation="NIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIF
                     SGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSM
                     MFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELR
                     KLVEYGII"
     gene            496..>1727
                     /locus_tag="U4B05_002932"
     CDS             496..>1727
                     /locus_tag="U4B05_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN2052013.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKIT"
BASE COUNT          560 a          226 c          273 g          668 t
ORIGIN      
        1 aaaacataca atcaatcttt gggatagaag aagtttttgt cggagataaa tgtatctttt
       61 ttgatcaacc tttattatct gatggttatg gcatagatga aacagatatt ttcgctaaag
      121 taaatagtat attttctgga aaaaaagaaa ctttaattaa gcttcatcct agagcaagga
      181 caaatcgtta tggtgaaaaa aacatattga attccagttt accgtttgaa gtcttgctac
      241 ttgaaaatga cttctcttca acacttttga ttagtcctat atctacaatt tctttttcac
      301 catctatgat gtttgaaaag acgatcaact ctattatgtt ggctaaatta attttaaatg
      361 aatatacagc attatctgaa gataaaaaag aaacattgac aaatattgtt cttttctgtg
      421 aacattataa ccgtctatca gaagaaaaaa ttttactccc agaaaactgg gcagaattaa
      481 gaaagttggt agagtatggg attatttaac acactttcca agtcaaaatt agttaagtcc
      541 agtttctggt atactatagg gaattttttt attaagggaa tttcattcat aactattcct
      601 atttttgtaa atattactac tcaagcagaa tatggtttga ttaataattt cacttcattt
      661 gtatcaattt tttcgattct tgttgggcta agcctaaatt cggcaattaa taatgcaagt
      721 tttgactata aagatgacat aaatgaattt atgtcatcgg tactttctct agctacactt
      781 tcgttaattg tgttcttatt aatagggaac atatattttt tgttttcaaa tgcttttttt
      841 gatatgaacc aacttgtatt taatttgatg ttattacaaa gttttgctag ttttgtaatt
      901 agttatatcc agtcttattt tacgattaac tttaaccatt ttaaatattt gattttatct
      961 tttatatcta cttttttgaa tattgcaatc tctgtagctt tgatgcagac attatatttt
     1021 gatgataaat atgttggcag agtagtcggc ggaacactgt catttgtgtt gataggttta
     1081 gtaatttatt taagaataat gataaagggt gctcattttt ttgataaaaa acagtggaga
     1141 tacgctttaa aaatctcttt gccgctaatc ccacattcct tagcaaatat agttttagca
     1201 caattcgata gaattatgat taattcttat ttaggtcctg ctgcgggggg aatatattcc
     1261 tacattagta acataggtat tattctaagc gtaatatgga tgtctactaa taacgcctgg
     1321 gttccatggt tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa gaaagttagt
     1381 aactattatt tagtagtctt tactttgatt acgatgattg taatggtagt ttcgattgat
     1441 ttagcaaaat ttatggctcc gaaagactat tattctgggt tatctttagt ggttccgata
     1501 accttaggct atttctttca gtttttatat agtttaccag ttaatgctga attttatgag
     1561 aaaaaaacat cgtttattgc gattggtaca gtagcaagtg ctatagttaa tatactatta
     1621 aacatgattt tcatacctag gttaggaata attgctgctg gatacacaac cgtatttacg
     1681 tattttttac tttttatttt tcattataat atcgctaaaa aaattac
//
LOCUS       DASJST010000263         1713 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.270, whole genome shotgun sequence.
ACCESSION   DASJST010000263 DASJST010000000
VERSION     DASJST010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.270"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            66..1625
                     /locus_tag="U4B05_002933"
     CDS             66..1625
                     /locus_tag="U4B05_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN2052014.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT          460 a          313 c          364 g          576 t
ORIGIN      
        1 tttatggatt tttccttgtc aattcgttga tttcgcgcta aattaaaaga aagcgaggcg
       61 tttgaatgga aaaaaaagga aaaatcaaga aattagataa gactcagata tattttattc
      121 taaaaggaat atttgttgga ctgctgacag gaatcgtcgt tagcctgttt cgattatcta
      181 tcgaaaagct aagcgattta gttcgaagta tctatgaaat gagtcgggaa cagccgattt
      241 acttaattgg cctagctatt tgctgcatcc ttgcagcatt tttcgtgggt tatctggtca
      301 aaaatgaacc ggatatcaag ggaagcggga ttccacaagt agagggacag cttcgcgggg
      361 aattatcaat gaactggttc tctgttttat ggaaaaagtt cattggcggt gtactttcag
      421 ttggtgcggg cctttttcta ggtagagaag gaccttctat acagcttggc gcatccattg
      481 gacaaggagc tggacaattg tttcggagtc catcttcaga agaaaagatt ttaatttcta
      541 gcggtgcaag tagcggactt gcagcagcat tcaatgctcc tatcgcagga ttactctttg
      601 ttttagaaga aatccatcac tccttttctc cacttgtttg gctgacatca tttgcttcag
      661 caatcactgc taacttcgtc tctctttact ttttcggctt gagacctgtt ttatatctag
      721 gtgaccttcc ttctttgccg ttgaaatatt atggttcctt agtgatctta ggagtacttc
      781 taggcatctt aggttttatt tatcagaatg tcttgctggc tttgccaaaa ttttacaacc
      841 gattgccttt accagcttat tttcacgggt tcgttccttt cttgctgatt ttgccaattg
      901 gttatttatg gcctagtact ctaggcggag gcaatcaaat cgttttagct tttggacatg
      961 cttccttgcc aatcattatg attatcggtt tgtttgttct tcgattcgtt ttttccatga
     1021 tttcttacgg tgggggtctt ccagggggga ttttccttcc gatattgaca ttgggggctt
     1081 taatcggtag cttttatgga aacattatgg ttgatttcgg gatggatcca atctacatca
     1141 aggattttgt cgttattgcc atggctggtt atttcacagc gattggaaaa gcaccgctga
     1201 cagcaattat tttagtgaca gaaatggttg gctctcttaa tcatctgatg cctttaggat
     1261 tggctgctct tgttgcttat atcgtcaatg accttctcgg cggtaatcca atttacgaat
     1321 cattattgga acgtctactt ggaaatactc agaccgcctc actaaaaggt gcaaggacaa
     1381 cttttgattt tccagtaacg gcagagagtt ctttagacgg attaatggta cgagacttca
     1441 attggcctaa aaatatgctg ctaatctcta tacgcagagg aaatcaggaa atcttgactc
     1501 acggtgatac gatcatgaat gtaggagata ttttggttat cttgactgat gaagcaaact
     1561 tgccaagaat caagcaggaa atcgaggcta aatcttctat cacaaatttg aagcaatcat
     1621 tttaacgaac atggaaaaac tattttacaa caactattca tctacactac ttgattctat
     1681 atgattctat aaaaaaagaa gaggctggga aaa
//
LOCUS       DASJST010000264         1700 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJST010000264 DASJST010000000
VERSION     DASJST010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.271"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..987
                     /locus_tag="U4B05_002934"
     CDS             28..987
                     /locus_tag="U4B05_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2052015.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(974..1579)
                     /locus_tag="U4B05_002935"
     CDS             complement(974..1579)
                     /locus_tag="U4B05_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052016.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          581 a          300 c          262 g          557 t
ORIGIN      
        1 cgaaagaatt ataaggagtg agtacaaatg acctataccc atcttacacc aactgaactc
       61 gtaatgatag aagcttattt caatcaatca cactctgttt caaaagtagc ccatctggta
      121 caacgttctc gacaaaccat ttataatgtc tatcgttttc ttaaatctgg tggaagcgca
      181 ataagctact atgaacaata taagaaaaac aaacgtaact gcggaagacg tccgattgtg
      241 ttaccagatg ggcaacaaga atacattcaa aaaaaggtgg ctcaagggtg gactccagat
      301 gtccttattg gtcgagcaga gtttcctatt gcttgttcgg tacgtacact atatcggatg
      361 tttaaagata aaacctttga ccctcacgat ttaccaatga aagggaaaag aaaaccaaac
      421 ggacataaag aaaaaagagg aaaacaagct tttcgccgtt cgattcagga tagaacaact
      481 gattatatac agttcaatca tgagtttggt cacctagaag gtgacaccat tgtcggtgga
      541 aaacataaaa gtgcggtgat cacgttagtt gagcgattat ctaaagtgat catcaccttg
      601 aaacccgctg gtaggcaagc tatagatatt gagaatagac tgaatcaatg gtttcaatct
      661 gtaccgaaac acttgtttaa atccatcaca tttgattgtg gaaaagagtt ctcgaattgg
      721 aaaaagatta gtaataccaa tgatatttcg atttatttcg ctgatccagg aacaccttct
      781 caacgaggac taaatgaaca ttcgaatggc ttattacgta aagatggctt atataaagct
      841 atggatttta ataaggtatc cgaaaccttt atccaatcgg tggcatcaaa acgaaatcat
      901 attcctagaa aatcattaca ctatcgcaca ccattggaag tatttttgag ttacgtaggt
      961 aaagatgatt tgtctagctt aatttgacaa acgaaacttt gtaaaaatat tcgattgaat
     1021 attctatttg gcacgactca cttggcagta tatgtatgtc aatatttttc atctgtttca
     1081 taggtatttc aaaatctaaa ctattaaaaa catataattc cctgtaatta tttacatcta
     1141 actctactaa atttatcttt ggaacaatag atcctccacc cattccacca taataaaaaa
     1201 aatcttgttc tctatcaaaa tagcacatat taagctcagt ttgtttatca tcctcaataa
     1261 ttttataaat caaacctata taatcatgaa tgctggcaaa aatttttttt aagtcaagtt
     1321 ctaaatcttc ttcgaaaaca catttaattt ctcctactga taattgatac tctatattac
     1381 atgacttaat tagtttttta gatcccccct ccagataggc atatgtttca ataattatgt
     1441 tctttttatt gcttggcaaa tttccgttat ttattgcata aattaaaaat gactgtctat
     1501 ttagcccata atctaataca acttcttcat actcatattt ctttaaatat ttaatatgaa
     1561 aatatgctga attaatcaaa actcgagtgt tactgttgtt atttattgtt gttctattgt
     1621 gtataaaatt gtataggttg tcatattttc ttggtttagt gaactactca ccacttagct
     1681 aaacctaacg gtttcttacg
//
LOCUS       DASJST010000265         1699 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJST010000265 DASJST010000000
VERSION     DASJST010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.272"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(102..644)
                     /locus_tag="U4B05_002936"
     CDS             complement(102..644)
                     /locus_tag="U4B05_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052017.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(957..1508)
                     /locus_tag="U4B05_002937"
     CDS             complement(957..1508)
                     /locus_tag="U4B05_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2052018.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
BASE COUNT          484 a          337 c          312 g          566 t
ORIGIN      
        1 ataacccaca tgaatgattt gaaatcgtct taaaatgctt tattttataa cccaaaatag
       61 aataattact tcaaaatgac gtcgcatatc actattaatg attaatcgat gacttccata
      121 atcattttaa gttccccgga tgttaaatcc tcgctaaaaa tgccagtaac tttttgtcca
      181 ggtccaattt cctttgatga taggtttaac aaagagatag ggatatagat taatccgttt
      241 tgtttcaaag acagagccaa tatttttttc cttgttcgaa tagctgcatc atagcgaaga
      301 gaagagtaaa cacctctgcc aacaatagaa atatgcttcc ccaataataa atcaaggcta
      361 cctattactt gtaatttctg aaaggtcgtc ccatccgttt tgatttgaat atcctgtaaa
      421 tttattttcc tgtctaaggc atcttgaaaa aacaagtgtg tgcctctgcg ataatcaatg
      481 ccacagaggt gcataaaatt agttgtcgaa aaaataatcg ctaccgttga gacttctccg
      541 acaaaggata cttttttatc tacaaaatgt tcttgtaaga gatgagcagc cttataaatc
      601 gcgtttaact gtctggtaat ttgggttgtc tcttttatag acatgcgttt gtattctttt
      661 tttgccgcgt gcgaagaatg ctcctttttc ccaataaaaa aagctgctca aaagagcagc
      721 cttttgtaca ggtttagttt tggtgcttgc cacccgccgg acccttacgt tccggatttg
      781 tatcgagttt agttttggtg ctcgccaccc gccggaccct tatgttccgg atttatatca
      841 agttgactat gagcctgctc tcatttgtat atataatata ccaaaaaaag gttgatttgt
      901 ccaatactgt gggtgattat aaccgtctta ctgtgcttat ctattatata atacaatcaa
      961 aaacctaacc gaaaatttaa taacgtttca ctgatttcat cttcattaat tccgatgtag
     1021 cgtttggtaa tcttctcgct gctgtgacca aagatttcca ttaaggtggc cacgtctttg
     1081 gttttcttgt aatagtggta accaaaggtt tttcgtaggg tgtgggtgcc gatatcgtcc
     1141 cttcctaaca gcttcgcgac cttttgaaac atctggtaga ccgtattgac ttctaaatgg
     1201 ccgcccttag tactgggaaa caagtaagcc tccggatcta agggtgcggt atagccgtga
     1261 atcaagtcct gtaagctgct taagtataaa atgcgtgttt tccctgtctt ttgctccacg
     1321 atgcgcgggt tttttgaggt aatcacgtct tttttcttga gtttaacgat gtcggacatg
     1381 cgcagaccgc tgttaattcc aattagaaac aagaaaacgt cacgttccgc atttttattc
     1441 cgtcgcaggc aaaataaaaa atcattaatc tcctgttgcg accgtagtgg ctgaacatta
     1501 tagcccatga caatccctcc tttttagtct gttcgaatac atgattatag ttattacaac
     1561 acgaaatcgt gtgtttaggg ggagtaaaaa aaaagaaccc tataaggata ggattcttga
     1621 tacatgattt tatccaattt catgtatctt atattcaacc cgcgccacaa gagtatggtt
     1681 gatatatcat tttgacttt
//
LOCUS       DASJST010000266         1699 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJST010000266 DASJST010000000
VERSION     DASJST010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.273"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..341
                     /locus_tag="U4B05_002938"
                     /pseudo
     CDS             123..341
                     /locus_tag="U4B05_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            601..855
                     /locus_tag="U4B05_002939"
     CDS             601..855
                     /locus_tag="U4B05_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2052019.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            856..1173
                     /locus_tag="U4B05_002940"
     CDS             856..1173
                     /locus_tag="U4B05_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2052020.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          595 a          253 c          321 g          530 t
ORIGIN      
        1 atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctacataga gttactttca
       61 atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt gatgaaattg
      121 ctattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat tggtctaatg
      181 gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa aataaagtta
      241 ttccagtatt gctatatgga acacataaca atttttatga attagccgag aacactcgca
      301 actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta tatcaatcaa
      361 ccgaccgaca tatgttaaat ctatatataa taaaaatacc tacttaaatg gatgttaacg
      421 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
      481 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
      541 gcaattgtcg ttataacatg ttataattta tttatcaaaa actaaaagaa gtgtgataac
      601 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
      661 tctcttggat taaaaagcgg agacactatc caagccaaac aagaaggcaa cttattcata
      721 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
      781 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
      841 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt ccagaccagc
      901 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
      961 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     1021 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     1081 agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt
     1141 ttccaatcta aacaaatgaa actaggcttt taacaataat taggcaaaag tcgctcatcc
     1201 tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat
     1261 tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga
     1321 tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat cgcttggtga
     1381 ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat
     1441 ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact
     1501 tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc
     1561 aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat acatccctgc
     1621 tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa tgggataaaa
     1681 tataggtaca ataactatt
//
LOCUS       DASJST010000267         1699 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.274, whole genome shotgun sequence.
ACCESSION   DASJST010000267 DASJST010000000
VERSION     DASJST010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.274"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="U4B05_002941"
     CDS             complement(<1..182)
                     /locus_tag="U4B05_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2052021.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="U4B05_002942"
     CDS             517..951
                     /locus_tag="U4B05_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2052022.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="U4B05_002943"
     CDS             974..1294
                     /locus_tag="U4B05_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052023.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="U4B05_002944"
     CDS             1435..1626
                     /locus_tag="U4B05_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052024.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          641 a          256 c          361 g          441 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattca
//
LOCUS       DASJST010000268         1698 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.275, whole genome shotgun sequence.
ACCESSION   DASJST010000268 DASJST010000000
VERSION     DASJST010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.275"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            155..1633
                     /locus_tag="U4B05_002945"
     CDS             155..1633
                     /locus_tag="U4B05_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2052025.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          621 a          233 c          304 g          540 t
ORIGIN      
        1 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
       61 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      121 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      181 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      241 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      301 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      361 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      421 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      481 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      541 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      601 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      661 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      721 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      781 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      841 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
      901 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
      961 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1021 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1081 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1141 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1201 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1261 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1321 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1381 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1441 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1501 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1561 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1621 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1681 gtttttttca aagaaaag
//
LOCUS       DASJST010000269         1671 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJST010000269 DASJST010000000
VERSION     DASJST010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.276"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..374
                     /locus_tag="U4B05_002946"
     CDS             <1..374
                     /locus_tag="U4B05_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HEN2052026.1"
                     /translation="GVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISV
                     PPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAY
                     SVLLSRAQRRFEGTLRYSLGY"
     gene            430..927
                     /locus_tag="U4B05_002947"
     CDS             430..927
                     /locus_tag="U4B05_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2052027.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(964..1647)
                     /locus_tag="U4B05_002948"
     CDS             complement(964..1647)
                     /locus_tag="U4B05_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052028.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          582 a          285 c          303 g          501 t
ORIGIN      
        1 caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat cttcccaata
       61 tcctagggga caaaggatat attagccata aaatccatga aaagcttgcc cttaagggca
      121 ttactatatc cgttccgcca cgtaaaaaca tggataaatc agaaaaacta gaccacagct
      181 tacttggaaa acaaagaaaa acagttgaga ctgttttttc ttccttagaa aaattaggtt
      241 gtcaaaattt caactcacgt tctgttaaag ggctagagag tagatttgaa agcatattac
      301 tggcttacag tgttctatta agtcgagcac aacgacgttt tgaaggaact ttgagatatt
      361 ctttaggata ttaaaattaa ctagcaccac gggtgattta tagtatacta tgtcaaaagg
      421 aggagttaga tgaacaatca aatcacacta gcgtatttag attatgatga tctagatgct
      481 ttacaagttg taagtgttgt cagtttttat gaatcgttta tcgatggcgc agatccactt
      541 gatatgcaag gatatttaca gacacaattg acaactgaaa tattggcagt agaattggca
      601 cagtcaacca gtaaatttat tggtattaag gatcatcaaa tactcattgg ttatatgaaa
      661 gtcaatgatg aaaaagatgc tatagagatt caacggctct atctgctcaa agattatcaa
      721 aacaaagggt tagggcaacg gttactcgat gaagcgaatc gttatgctaa aacgaaacaa
      781 aaacgttatt tacgtttgac tgtttatgaa aaaaatcatg caggtattcg tttttatgaa
      841 cgtcatggat ttaaaaaaat cgggatcaaa cattttcctt taggaaaaca agatcgtatt
      901 tgtccgattt tagaaaaaga aatctaaaaa aacgtcctca cttttgatgc tgaggacgtt
      961 tttttagact ggtaatggaa ccataattcg ctttcctttt tgataaatcg ctaattccgt
     1021 cacaccattt gcttttaaca gagcaaaaag atcatcgaaa tggaaacgga aatgttcaag
     1081 gtaatgccca tctgatccaa cactaaacaa ggtaccacca cgtgaaagat ataaatcgat
     1141 gacatattca tataacgcta aattgtcata caagtaagcg ctcttagcat ttaattcgaa
     1201 tgccaattta tttttgatta cttgatctag aatcggtaaa aactgcgctt catattgttt
     1261 aaattcagca acggatagac caaatacccg aaaaccatag tcaaaatgag caaagacatc
     1321 tgcttctata acaactagtg cttctgaaag ttgtgcaaaa tactgaggaa tcacaatagc
     1381 aggatccata tctttgactt cgtcatctaa ataatcaaat tgcccattat gatgcacact
     1441 gagtaatttt aaatcgtaat ctttatcggc taaatagtcg atgatccgat ccttttcaga
     1501 agcgatgtaa ccgattttga tgccttttaa aacacgattg ccaaaacgct tatttaagct
     1561 atcgattgtt tgactgtaca tttcataatc ggggatatca tcgactttag taacaggatt
     1621 ggctaagtca aaatgttcag ttgtcacgac gtaaccatcg tagtttgtta a
//
LOCUS       DASJST010000270         1664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJST010000270 DASJST010000000
VERSION     DASJST010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.277"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4B05_002949"
     CDS             131..505
                     /locus_tag="U4B05_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2052029.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U4B05_002950"
     CDS             complement(619..1248)
                     /locus_tag="U4B05_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2052030.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U4B05_002951"
     tRNA            complement(1382..1454)
                     /locus_tag="U4B05_002951"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DASJST010000271         1659 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJST010000271 DASJST010000000
VERSION     DASJST010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.278"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..417
                     /locus_tag="U4B05_002952"
     CDS             <1..417
                     /locus_tag="U4B05_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2052031.1"
                     /translation="VIENFASKIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAI
                     KFTQKEGTIKFSISKNKDHVLLIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQG
                     IGLAIVKSVIEAHKGSITVKSKLGLGTKFVIKLPFQ"
     gene            432..638
                     /locus_tag="U4B05_002953"
     CDS             432..638
                     /locus_tag="U4B05_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052032.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            659..1255
                     /locus_tag="U4B05_002954"
     CDS             659..1255
                     /locus_tag="U4B05_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HEN2052033.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT          586 a          228 c          339 g          506 t
ORIGIN      
        1 gttattgaaa atttcgccag caaaattgag tcaaaaaacc ttcatgttga aatccaaggg
       61 gataacatca atatatctgc agataaagat aaaatgaacc aagttgttat caatcttttg
      121 aataatgcta ttaaatttac gcaaaaggag ggaaccatta agttttctat aagtaaaaat
      181 aaagaccatg ttcttttaat tgttgaagac aacggcatcg gtatagaaaa agatcagcaa
      241 cttcatattt ttgatagatt ttatatggct gacccctcca gaagtagagc tttagggggg
      301 caaggtatcg gattagctat tgtaaagagc gttattgaag cgcataaagg tagtataact
      361 gtaaaaagta aattaggtct aggaactaaa tttgttataa agttaccatt tcaataaagg
      421 aggagagtta tttgtttcaa aaacagataa aaattgctaa actagtatgt gaatgttggg
      481 caaaaaaaat attttttcaa ctgatttcga tagatagtgt cgaaaaagtt catttagata
      541 tcttatcaaa ttctgtagta atcttttcta agaaggactt atcaatagct acaattaaaa
      601 aagaagtttt tggattacaa atttatgaga tgacctaaat gtgattaatt aaaggaggat
      661 gaagacagtg aaaaagatga caaaattagt atttgcaagt cttggaatta ttgtattttt
      721 aggagcgtgt agcaatcaaa gtgaatcgaa taattctaat agcacaaacg aggaatcgac
      781 ttctacagcg tctagtgaaa tgaatagtat ggagggaatg aatcacgaag gtatggttcc
      841 ttcttcaatg aaagatgctg cgaaccctaa attcccggtg ggaagtaatg ttatactgct
      901 aggcgatcat atggaaggga tgaaaggtgc aaatgctcaa gttgtagggg cctttgatac
      961 tacaatgtat gaagtaagct atgagccaaa aactggggga ccaatggtta acaatcatcg
     1021 atgggtagta caagaagagt taaaagatac tgagactgta gcaaatgagg gcgatactgt
     1081 tatcttgaat gccgatcaca tggatgggat gatgggagct gaagcaaaag tagataaatc
     1141 tattacagga actgtttacg ttgttaatta cactccaacc gatggtcaag aagaagttaa
     1201 gaatcatatg tgggtaactg aagatgagat ggaatatgac gaaaataatg agtaatttta
     1261 tatactaaac caacaagctg gtacatggtc ttcgtattct caacatgagg aatatgagct
     1321 ctttgttaat aaggactgat gagttgtgca aaatcaaatc tgggtcagaa cgaacctcat
     1381 tctggctcag atttttggct cttcgacatt tagtgctggt agagagacaa ctgaaaattt
     1441 agtcataacc agttaggcag agtgcttaac tggtttttgt tcgcatttat tattcttttg
     1501 atattggctg cttgcccttc aattttattt ggtttcttaa tcaactcttc atataacccc
     1561 aaatcatata ttaatgaggt gcctcctatt cctataaatc tatctcttct ttaatagtat
     1621 gagaaaagtc tactcttgta gatttaaatt gtcaacaaa
//
LOCUS       DASJST010000272         1643 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJST010000272 DASJST010000000
VERSION     DASJST010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.279"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..650)
                     /locus_tag="U4B05_002955"
     CDS             complement(<1..650)
                     /locus_tag="U4B05_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2052034.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="U4B05_002956"
     CDS             975..1391
                     /locus_tag="U4B05_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2052035.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..>1643
                     /locus_tag="U4B05_002957"
     CDS             1388..>1643
                     /locus_tag="U4B05_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2052036.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNT"
BASE COUNT          559 a          255 c          293 g          536 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caa
//
LOCUS       DASJST010000273         1539 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJST010000273 DASJST010000000
VERSION     DASJST010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.280"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..1518)
                     /locus_tag="U4B05_002958"
     CDS             complement(52..1518)
                     /locus_tag="U4B05_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN2052037.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          502 a          326 c          289 g          422 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattcttta
//
LOCUS       DASJST010000274         1534 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.281, whole genome shotgun sequence.
ACCESSION   DASJST010000274 DASJST010000000
VERSION     DASJST010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.281"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1003
                     /locus_tag="U4B05_002959"
     CDS             <1..1003
                     /locus_tag="U4B05_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2052038.1"
                     /translation="KDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNILFSSGVF
                     TIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIF
                     SGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALN
                     KLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWL
                     RERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDGFINSE
                     DSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1013..>1534
                     /locus_tag="U4B05_002960"
     CDS             1013..>1534
                     /locus_tag="U4B05_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2052039.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKT"
BASE COUNT          632 a          146 c          257 g          499 t
ORIGIN      
        1 aaaagatgct aaagtagtta gcggagaaca aagaataaat agagaagatt tatctgacga
       61 atttaagaat gttgtttcac tcgaagctac aaataatact aaaagaaata ttttatttag
      121 tagtggagta tttacaatta aagaaggtaa aaatatagaa gaaaatgata agaattcaat
      181 tattgttcat gaagaatttg ctaaacaaaa caatctaaaa ttaggtgatg aagttgatct
      241 tgaattacta gatattgaaa aaagtggaaa aataaaaagt cataaattta aaattatagg
      301 gatcttttct ggtaaaaaac aggaaacata tacaggatta tcttctgatt ttagtgaaaa
      361 tatggtgttt gtagattatt caacaagcca agagatatta aacaattcag aaaataatga
      421 aattgcaaat aaaattttaa tgtattctgg tagtgcagaa tctacagact tagccttaaa
      481 caaattgaaa gagcttaaaa ttgatgagtc aaagtatttt gttgaaaaag attctaatgc
      541 gtttgaagag tctttagagt cagtgagtgg aataaaatat ataataaaag taatgactta
      601 ttctattatg ttgggtggga tggttgttct ttcattaatc ttgattctat ggttaaggga
      661 aagaatttat gaaataggta tattcttatc tattggaaca tctaagatac aaattataat
      721 gcaatttata ttcgagttaa tattcatatc gataccaagt ataatatctt ccttattttt
      781 aggaaatgta ttactaaaag taattgtaga tggattcatt aactcagagg attcaatgat
      841 ttccggtgga agtttaataa ataatagtag ttttatgtca aatataacaa cacttggaca
      901 aagttattta atattaataa gtattattgt tttatcagtt gtatttgctt cttcattaat
      961 gttaatcaag aagcctaaag aaatcttatc aaaaataagt taggagaaaa taatggatat
     1021 attagaaata aagaatgtag catatagtta tgcaaattct aaagaaaaag ttttgtcagg
     1081 agtaaatcaa aaatttgaac ttgggaagtt ttatgcgata gtaggaaagt caggaacagg
     1141 aaaatctaca cttctttcct tacttgctgg acttgataaa cctcaaacag gaaaaatatt
     1201 gtttaagaat gaagatatac aaaataaagg atatagtaat catagaaaaa ataatatatc
     1261 tttagtattt caaaactata atttaataga ttatttatca ccaattgaaa atattagatt
     1321 agtaaataaa tcagcagatg aaagtatctt gtttgaacta ggtttagata aaaaacaaat
     1381 aaaaagaaat gttatgaaat tatctggtgg acagcaacaa agagtagcta ttgctagagc
     1441 attggtatca gatgctccaa taatactagc tgatgagcct actggtaatc tagacagtgt
     1501 tactgctgga gaaataatta atatattaaa gaca
//
LOCUS       DASJST010000275         1526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJST010000275 DASJST010000000
VERSION     DASJST010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.282"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..734)
                     /locus_tag="U4B05_002961"
     CDS             complement(<1..734)
                     /locus_tag="U4B05_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2052040.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
     gene            complement(731..1150)
                     /locus_tag="U4B05_002962"
     CDS             complement(731..1150)
                     /locus_tag="U4B05_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2052041.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="U4B05_002963"
     CDS             complement(1220..1360)
                     /locus_tag="U4B05_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2052042.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1353..>1526)
                     /locus_tag="U4B05_002964"
     CDS             complement(1353..>1526)
                     /locus_tag="U4B05_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052043.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          417 a          327 c          236 g          546 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtgg
       61 gcgtaatttg ttccatctgt cagtgtagga tttgctgcac ctgctctagt tggatcaatg
      121 actggtttat catctgaagt tgtcggattt tcatcggtaa atccatgagc taaatcatat
      181 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      241 ccccagatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggcgtt
      301 tctaatgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgtgtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacttca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagaat gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaaaaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccact
//
LOCUS       DASJST010000276         1523 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.283, whole genome shotgun sequence.
ACCESSION   DASJST010000276 DASJST010000000
VERSION     DASJST010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.283"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..626)
                     /locus_tag="U4B05_002965"
     CDS             complement(<1..626)
                     /locus_tag="U4B05_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2052044.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDR"
     gene            774..>1523
                     /locus_tag="U4B05_002966"
     CDS             774..>1523
                     /locus_tag="U4B05_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2052045.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPET"
BASE COUNT          566 a          281 c          259 g          417 t
ORIGIN      
        1 cggtcaagta cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct
       61 tcatcataaa attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca
      121 tattgaattt gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata
      181 gacaaaccgt acaatgctaa aaatgcatta atctttttca ttttcgtata caaataagag
      241 gcactatagt ttgtttgctg acttaaactg ataatcgaat ttgtctgaca gctgaacaaa
      301 gaagtcaaga tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag
      361 cagtaagccg gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata
      421 tgcagttctg ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa
      481 aaatttgatt cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga
      541 tgttcagata ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagagaaac
      601 gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac
      661 ttgaaaatat tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag
      721 aatctctact atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat
      781 attcaaccat cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga
      841 cagtccacac agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca
      901 atcgaattac ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa
      961 aaggacaaac agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac
     1021 tacaatcagc tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga
     1081 acactttgac tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg
     1141 acttgttaaa accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc
     1201 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag
     1261 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     1321 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     1381 atacagaaac tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa
     1441 aaccagatgt agacccagaa gaaaaaccag atgtgactcc agaaccagat acggattctg
     1501 gaaaccaaac agtaccggaa aca
//
LOCUS       DASJST010000277         1483 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.284, whole genome shotgun sequence.
ACCESSION   DASJST010000277 DASJST010000000
VERSION     DASJST010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.284"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..444
                     /locus_tag="U4B05_002967"
     CDS             <1..444
                     /locus_tag="U4B05_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2052046.1"
                     /translation="IFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARR
                     NPYFNMVKRIENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCG
                     IIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            449..1396
                     /locus_tag="U4B05_002968"
     CDS             449..1396
                     /locus_tag="U4B05_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN2052047.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          498 a          208 c          294 g          483 t
ORIGIN      
        1 atatttgatt taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt
       61 gaagaattaa tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt
      121 tctgcaagac gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata
      181 gttaataaat caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc
      241 tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa
      301 tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga agaagatttt
      361 aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc
      421 tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg
      481 cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga
      541 tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga
      601 gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta
      661 tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac
      721 acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc
      781 tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt
      841 accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc
      901 tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg
      961 ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc
     1021 tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt
     1081 tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact
     1141 tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc
     1201 aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact
     1261 tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat
     1321 ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa
     1381 aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc
     1441 aataaggact gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DASJST010000278         1483 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.285, whole genome shotgun sequence.
ACCESSION   DASJST010000278 DASJST010000000
VERSION     DASJST010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.285"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            64..972
                     /locus_tag="U4B05_002969"
     CDS             64..972
                     /locus_tag="U4B05_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2052048.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="U4B05_002970"
     CDS             972..>1483
                     /locus_tag="U4B05_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052049.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DASJST010000279         1480 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.286, whole genome shotgun sequence.
ACCESSION   DASJST010000279 DASJST010000000
VERSION     DASJST010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.286"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            64..972
                     /locus_tag="U4B05_002971"
     CDS             64..972
                     /locus_tag="U4B05_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2052050.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="U4B05_002972"
     CDS             972..>1480
                     /locus_tag="U4B05_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052051.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DASJST010000280         1425 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.287, whole genome shotgun sequence.
ACCESSION   DASJST010000280 DASJST010000000
VERSION     DASJST010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.287"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..1392
                     /locus_tag="U4B05_002973"
     CDS             100..1392
                     /locus_tag="U4B05_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2052052.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          258 g          412 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DASJST010000281         1424 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJST010000281 DASJST010000000
VERSION     DASJST010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.288"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U4B05_002974"
     CDS             complement(150..1337)
                     /locus_tag="U4B05_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN2052053.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DASJST010000282         1403 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.289, whole genome shotgun sequence.
ACCESSION   DASJST010000282 DASJST010000000
VERSION     DASJST010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.289"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            306..1391
                     /gene="xerS"
                     /locus_tag="U4B05_002975"
     CDS             306..1391
                     /gene="xerS"
                     /locus_tag="U4B05_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN2052054.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          547 a          234 c          199 g          423 t
ORIGIN      
        1 tatatacatt catatacgct tgttaatatc aaatcaaccg tagtctctct tatgtctaat
       61 tatatagaaa gattgttctt tctatataat ttagacaatt taaaaaatta agattgcctc
      121 cttctgtttc ctttcctatt catgtatacc atttatttta aattattaaa tctataccta
      181 tttatctgct tttttgaaat aaattaataa atttcagagt ataccaattg acaaaacact
      241 ctataattgg tatagtccaa ttatagagat actcctctcc cctacttatc gaaaggatga
      301 ataacatgaa aaatacagaa atatatcaaa aaatagatac aatcatactt catatgcccg
      361 attatataaa agattttgtt caagatcgtg aggataaaga tcaatcacct cgaactactt
      421 tagaatatct aaaaaattat aagctctttt atgaatggct tctgagtgag tctatcgttc
      481 ccgatacttc aatcacctcg ataagacagt taactgccta tgatctaaac ttgtacaaat
      541 ctcatttgaa acgtcgcaca aaggaaaatg taaaaaaaga gacaacaaaa actaagctca
      601 aagataataa taacttaggt ctatctactt ctactatcaa tagaaatatc acagcattaa
      661 aggttctatt taaatatctt tcgaaaagtt ccaataatcc tttaggaaaa ccatacttag
      721 aaaataatcc tatggatcaa gtagcaacta tcacagataa aactacgtta gctgcccgtg
      781 caaacgcaat cgagaaaaaa ttatttctag acgaagatac acaaaattat ctagattata
      841 ttgccaatga atataaaaaa actttgagca aacgagcttt gatctattat caacgagatg
      901 tagaaagaga cctggcgatc aatgcattga ttttaggatc tggattaaga ctctccgagg
      961 ttgtaaatat taatttagag gacctatctt tagaaaagaa taatgtggta gtaactagaa
     1021 aaggaaataa gagagacgca gtaaatatag cagcatttgc aatggaatat ttagctaatt
     1081 atctagctat tcggaaagaa cgatacaaag taacagaaaa cgaaaaagct ttatttctag
     1141 caatttatca aggagaagcc aagcgtatat ccggtattgc aatcgaacgg atggtagcaa
     1201 aatattcaaa aggattcaga gtccaagtca gtccacataa attacgtcat actttggcta
     1261 ctagactcta tcaacagaca aattcattgg tattaactgc ccaacaattg ggacatacaa
     1321 gtacaaatac aacaacttta tatactcata tagacaatgc agctacaatt gatgctttga
     1381 acgctttgta aatgtagtaa aaa
//
LOCUS       DASJST010000283         1393 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJST010000283 DASJST010000000
VERSION     DASJST010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.290"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            96..1391
                     /locus_tag="U4B05_002976"
     CDS             96..1391
                     /locus_tag="U4B05_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2052055.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          442 a          277 c          260 g          414 t
ORIGIN      
        1 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
       61 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
      121 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      181 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      241 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      301 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      361 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      421 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      481 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      541 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      601 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      661 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      721 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      781 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      841 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      901 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      961 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
     1021 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     1081 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     1141 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     1201 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     1261 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     1321 gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt
     1381 taaccaaata aac
//
LOCUS       DASJST010000284         1384 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJST010000284 DASJST010000000
VERSION     DASJST010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.291"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1342
                     /locus_tag="U4B05_002977"
     rRNA            <1..1342
                     /locus_tag="U4B05_002977"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          381 a          299 c          414 g          290 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 actt
//
LOCUS       DASJST010000285         1354 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJST010000285 DASJST010000000
VERSION     DASJST010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.292"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..870)
                     /locus_tag="U4B05_002978"
     CDS             complement(<1..870)
                     /locus_tag="U4B05_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEN2052056.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELI"
     gene            complement(876..>1354)
                     /locus_tag="U4B05_002979"
     CDS             complement(876..>1354)
                     /locus_tag="U4B05_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2052057.1"
                     /translation="IEIFKEFHAIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFL
                     GNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFI
                     SLNQSAKEWAREIANVDISHRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
BASE COUNT          422 a          249 c          156 g          527 t
ORIGIN      
        1 aatcaattcg tcacataatg gattgttact aggtatttta tcaaatgtct gtgctatttc
       61 atgatttatt ctctgtgcta aaacaatcat ataatctatc atcaaataat ccaccatcat
      121 atcattattt ttccaataat ttaaaaaaaa gtctctaatt atagcaaaca tccatctaga
      181 ctcttgatta catgctaatg aataacccgc aaaatatcct gatgctacgg atgcgtgagc
      241 ataatcaggc ctcctaatag accaaatatc gatatcaaaa tactcatctg gtattggttt
      301 tgtacaataa aatgtggcgt ctaaccacaa acctccatgt tttgctagca aagataatct
      361 taataaatca gaataatttg ttctcgtaat gattcctttg tttttctttt cttccaccca
      421 ttttggtata tcaacatatt gtctataatt atcttctgtt aaaattataa cagtatgttt
      481 ccctgcattt cgttttattg attttacaca agcttttaca atttccggcg attgatccaa
      541 tccctgccac caacaaaccc aaatgcagtt agaatatggt gattttgatt gattaattat
      601 ttgattttga ctataatcat atgttttcaa aaaatctgaa aaagtctttt cataatactt
      661 cagcattacc tcatgttttt ttaataaatg ttccttctgt tttttacttt cattatatcc
      721 attacgattc atgacttgaa tatcaatttt agcttgtaac gtggcaaatg cttccttcca
      781 agatgttatc tttgcaattc gtcttgttgc attcaattct tcggatatgc gatgtataat
      841 tctttttata cttgaatgtt tctgtgtcat attctttaac ctctttgtat taattcaaat
      901 aactcataat attttgattc taagattttg tatgctgttt gaatattata atctgccttt
      961 attaaaatcg aacgttctga atctcgatga gagatatcta catttgcaat ctctctcgcc
     1021 cattcttttg ccgattgatt taaacttatg aaattggtct tatcactgaa agcaacttct
     1081 gtaggaactt tctccgaaaa gaaacacggt aaatctgcaa attgagcttc aatacccact
     1141 aacggaaccc cttcaaataa tgatggcaac attaatacgt caaatgcttg ataaaatttt
     1201 tctatatcat ttctatttcc taaaaattta atatcatcat ctatgccact tttctttgca
     1261 taatttttta tttcttcttc catatcacca gttccaataa ttaataatac ggaattattc
     1321 ttaatagcgt gaaattcttt aaaaatttca atca
//
LOCUS       DASJST010000286         1328 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJST010000286 DASJST010000000
VERSION     DASJST010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.293"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..1257
                     /gene="rpsA"
                     /locus_tag="U4B05_002980"
     CDS             28..1257
                     /gene="rpsA"
                     /locus_tag="U4B05_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN2052058.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT          452 a          208 c          315 g          353 t
ORIGIN      
        1 agaatgttgg ttaggaggac aagtgttatg acagaatttg atcaaaatca agagaacaac
       61 cagtcaatgg aagacgcaat gaaaagcgta caagaagtaa aagttggcga tgttgttaag
      121 ggcgaagttt tagtcattga agacaaacaa gtgattgtcg gaatcgaagg ggctggagtt
      181 gaaggagtcg ttccggcaaa agaattatca actttgccgg tagaagatat cagcgaagta
      241 gtaaaagtcg gtgatgtatt agatttagtc gttatcagta cgatcggcaa agataaagaa
      301 aacggcagct atctactttc aaaacgccgt ttagatgcta aaaaagtttg ggaagatatc
      361 gaaagagatt tccaagaggg caaggtcatc gaagcacctg ttacaaatgt tgtcaaaggc
      421 ggtttagtcg ttgacgtcgg tgtacgtggt tttgtccctg catcaatggt ggaagatcat
      481 tttgttgcag acttttcaga ctataaaggc aaaacattga gctttaaaat catcgaaatc
      541 gaaccatcag aaaatcgttt gatcttatcg cataaagcgg tagtggaaaa cgaaagagaa
      601 gaaaagaaaa aagaaattct ttcaactatc catgagggag atgttctcag tggacgtgtt
      661 gcacgtctta ctgattttgg cgcatttgtc gacctaggcg gaattgacgg tctagtccat
      721 gtctcagaga tttcacatag tcatgtagca aaaccaagtg atgtattggc tgtcaatgat
      781 gatgtaaatg tcaaagtgtt gtctatcaat cctgaacaag aacgtgtttc cttgtcaatc
      841 aaagacacac agcctggacc ttggtcagaa atcgatacaa aagctccagt tggcacagta
      901 ttagacggaa cagttaaacg tttgacaagc ttcggtgcct ttgttgaagt gttccctgga
      961 gtagaagggc tagtccatat ctctcaaatt tcacataaac atattgcgac accacatgaa
     1021 gtgcttcatg aaggtgacga agtgaaagtg aaagtattag agatccatcc agaagaacat
     1081 cgtattgcat tgagtatcaa agctttagaa gaaaaaccag cttcagaaga agaaccaaaa
     1141 aatgttgagt cttatgaatt accagaagaa aatactggat ttactatggg agacatttta
     1201 ggtgatgcgt tgaaagaaga agtagaagaa aaagcaaccg atgattctga aaaataattg
     1261 attttgtatc tgtgactgag atgaaagcag ctgcttttgt ctcagttttt cttttgaaaa
     1321 gcgattag
//
LOCUS       DASJST010000287         1324 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.294, whole genome shotgun sequence.
ACCESSION   DASJST010000287 DASJST010000000
VERSION     DASJST010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.294"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..1217
                     /locus_tag="U4B05_002981"
     CDS             join(55..331,331..1217)
                     /locus_tag="U4B05_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2052059.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          478 a          234 c          265 g          347 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acaccggttg
     1141 gttaccgtaa ccagagattg gcgcagcgaa tccttgataa tgagctcgga tgtgctaacg
     1201 ctagcgaggc agtctaactt taactgttag ctcctgccga agatcgtcac atccgaggag
     1261 gctcattgtc aaggacaatc ggaatagcat acggaaagaa gttctgcacc ttataaaatt
     1321 tgtc
//
LOCUS       DASJST010000288         1316 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJST010000288 DASJST010000000
VERSION     DASJST010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.295"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..351
                     /locus_tag="U4B05_002982"
     CDS             <1..351
                     /locus_tag="U4B05_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052060.1"
                     /translation="NVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCI
                     CGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGI
                     KLNQETIKKLRIGG"
     gene            354..1184
                     /locus_tag="U4B05_002983"
     CDS             354..1184
                     /locus_tag="U4B05_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEN2052061.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLGSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDIDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLPI
                     "
BASE COUNT          529 a          195 c          278 g          314 t
ORIGIN      
        1 aatgtcttag caaatatcat tttagatttc atctttgaac atcatattcc tttcaaagaa
       61 ggatatgaga ttttaccagc gaatcaagag tattacttct acaaatgcat tacaaaaaga
      121 gtctgctgca tctgtggcaa aacaggagct gatatcgatc actttgacaa agcgctggga
      181 agacgaaagc gcaaggaagt tgatcattca gagtacacat ttgcagcact ctgcagaatc
      241 catcacacag agaaacacaa aataggtgtg atcaatttca aaaataaata tcaaatcaaa
      301 ggaatcaagt taaaccagga aacaattaaa aagttaagga taggaggata aatttggctg
      361 agataagttg gatcaaactt aaaactacta tgtttgacga tgaaaaaata cgattaatcc
      421 aagctgttcc tgagtcggat gccatcatcg ttatatggat tcgattacta gttttagcag
      481 gaaagactaa cgacgatggt ctgatatata tccagaggaa catgccttat accgaagaaa
      541 tgcttgctac attgttcggc aaaaacgtaa atacggttcg cctagcgcta actacattgg
      601 caaatttcaa catgattgat ctaggcagtg atggactgat tgccatcagt aattgggaaa
      661 aacatcaaaa tatcgaaggg atggataaag taaggctaaa aaacgctgaa agaaaccgta
      721 aatacagaga aagaaagaga caggaacgtc tcaaattgga aaatgacgtt agcgtgacgt
      781 cacgtgacgg tacagataaa gatatagaag aagatataga tatagataaa gaagaaaaga
      841 aaggtaagta ttctgacgaa cacttacgcc ttgctaaaaa gttgcaaagt aatttaactg
      901 aagattttcc aaaagaaatg aataaagtag atatcgaaaa atgggcagac acaatcaggt
      961 tgatggaaga aagagataaa gcatctatag aagcgattga gtatgtgatc aattggctac
     1021 ctacaaatga attttggttt ggaaatatta gaagtgctaa gaaactgcga gaaaaatttg
     1081 agaagcttaa attcgaaatc aaggcagata aaaataatca taaaaagcaa agtcaaaaat
     1141 tacagtacag caatcctagt gaatatgacg acttgccaat ttaaaaagga gatgcatcac
     1201 atggaaagcc tagcaagtac tatggaaaag ctaataagaa gagttttagt acaaagtgga
     1261 agatgcccag agtgcagcga acctttgtat agttggcgag ggaaaaataa ggacgg
//
LOCUS       DASJST010000289         1310 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJST010000289 DASJST010000000
VERSION     DASJST010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.296"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..>1310
                     /locus_tag="U4B05_002984"
     CDS             127..>1310
                     /locus_tag="U4B05_002984"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2052062.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LHT"
BASE COUNT          411 a          262 c          235 g          402 t
ORIGIN      
        1 tctataattt ctaggactga tggaaatttt ctatctgtcc tagttttttt gtaaaaaaac
       61 aaaaagacat acaaaatttc cggtagaata gagtctccta caacatcatt ctgggggaat
      121 tcttgtatgt ccatgaatca ttttatcaaa caactcttta ctattaaaga ctcaaatatc
      181 caaattcaat cctttgaaga aactttttat aaaagcagaa aaagtatcgt tattgaggga
      241 actctgatcc gtacctacag acgttgtccg tgttgtaaag attctacaaa gcaaatcgtt
      301 aaaaatggca agaagatctc tatgattctc ctgaatcgta gcggtaataa acggacctat
      361 cttcggttga agaaacagcg atacctctgc cgagcatgta aaaaatattt taccgctcgg
      421 acgtatctcg ttaccccatt ctgctttatt tctaagcaga ttcattacaa gatcttagaa
      481 gaattgacag agcgtcaatc tataaaagca attggtaaac attgcgatgt ttctgtgaca
      541 actgttcagc ggacattgtc cgccttgtca tcagatatcc gtcagaatct tgagtattta
      601 cctcggtgtc tattattcga tgagtttcgt tctctttcta catggcacgg taaattttct
      661 ttttcttgta tggacggtga gaccgggaaa ctgtttgaga tcttacctag ccgccgtaaa
      721 aaagatctcg ttgctttttt catgcgtttt tctctcaaag cgaggttggg tgtacattac
      781 atcgtaacgg atatgaacgc tccttatttc tcactagtta aagagtgttt tccaaatgca
      841 caagtgatcg tcgatcggtt tcacattgtt caacatctga accgcttctt cgatgcggtt
      901 cgaaaacgtg tgatgaaaac acttgatcaa aaagacccaa tacaagctaa acagtatcgc
      961 caattgaaat ccttattcaa attactttta aaatcagagg atcgtttaga ttacaataca
     1021 ctaacgaaaa gacgcaattt caattggaga ctattgactg aaacagaagt agttgaccgc
     1081 cttctaaaga tttctcctga acttaaaaca gcttatcgtt actaccaaga cctgcttact
     1141 gcgtatcagg aaaaagatcc agatactttt ttccagctgc ttcgagcaat gcctggagaa
     1201 gttccattgg aactgcacca tatcaaaaaa gcctttttca aatacgagaa agggattcgg
     1261 ctggcattac tgaagaagta ttcgaatgca cgactggaaa atctccatac
//
LOCUS       DASJST010000290         1294 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.297, whole genome shotgun sequence.
ACCESSION   DASJST010000290 DASJST010000000
VERSION     DASJST010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.297"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1244
                     /locus_tag="U4B05_002985"
     CDS             <1..1244
                     /locus_tag="U4B05_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN2052063.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DASJST010000291         1277 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.298, whole genome shotgun sequence.
ACCESSION   DASJST010000291 DASJST010000000
VERSION     DASJST010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.298"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(594..959)
                     /locus_tag="U4B05_002986"
     CDS             complement(594..959)
                     /locus_tag="U4B05_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052064.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1035..>1277)
                     /locus_tag="U4B05_002987"
     CDS             complement(1035..>1277)
                     /locus_tag="U4B05_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2052065.1"
                     /translation="SIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKYE
                     EGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          436 a          219 c          247 g          375 t
ORIGIN      
        1 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
       61 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      121 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      181 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      241 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      301 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      361 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      421 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
      481 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
      541 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
      601 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
      661 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
      721 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
      781 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
      841 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
      901 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
      961 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1021 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1081 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1141 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1201 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1261 atagcttctt cgatact
//
LOCUS       DASJST010000292         1276 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.299, whole genome shotgun sequence.
ACCESSION   DASJST010000292 DASJST010000000
VERSION     DASJST010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.299"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            57..929
                     /locus_tag="U4B05_002988"
     CDS             57..929
                     /locus_tag="U4B05_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2052066.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          384 a          231 c          305 g          356 t
ORIGIN      
        1 atgatatata gtcatttatt tagtgatttc attctaaaat aaagggagaa atgtctatgc
       61 tatacggtgc aattgaagca ggcggcacaa aatttgtgtg tgccattggc gatgaagaaa
      121 tgacgatcaa ggaaagagtg agctttccga caacgacacc tgaggaaaca atgcctttag
      181 tcattgactt cttcaaacaa taccaagcag atttagcagg gattgggatt ggctcatttg
      241 ggcctatcga tatccatcgc gattccgcca cttatgggta cattacctcg accccgaaat
      301 tggcatggca aaattttgat ttcatcggta ccatgaaaaa agaatttccg atcccgattt
      361 cttggacaac agatgtcaat gcggctgcct atggtgagta tgtcttcggc agtggcaaag
      421 gattgagcag tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt gccttacaag
      481 aagggcgctt tatcgaaggg tttagtcatc cagaaatggg gcatatgctc gtcgtccctc
      541 atccaaaaga tgactttgct ggcagctgtc catttcatgg caactgttta gaagggatgg
      601 ccgctggacc cgccatcgaa aaccgcttag ggaaaaaagg acaagaggtt gcagaagatg
      661 atccttattg ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat acaaccttga
      721 tgttttctcc agatgtgatc atttttggcg gtggtgtgat gaaacaacgc catatgctgc
      781 aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca cctgagttaa
      841 gcaaatatat cgtgacccca gcattagaag acaacgctgg aacgctcggt tgtttggcat
      901 tggctcgaga agcggtctta cattcataaa cgaaaaaagg ctcttcgaca tttagtgttg
      961 gtagagaaag atacaactga aaatttagtc ataaccagtt aggcagagtg cttaactggt
     1021 ttttgtttta cttggaagat caagccttga atgagataaa ttcttgctcg aaagttatgg
     1081 aaatttcgat agccgtaggc cacttgtatt gtgcaatagt tttttacaaa aaaaaggggg
     1141 tgcggatatt tttttggacc tttttaacaa ataggttctg ttgcaaagtt ttaaatctac
     1201 tatcagataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg agtcatttta
     1261 aaggaaagca atttca
//
LOCUS       DASJST010000293         1250 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.300, whole genome shotgun sequence.
ACCESSION   DASJST010000293 DASJST010000000
VERSION     DASJST010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.300"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(58..456)
                     /locus_tag="U4B05_002989"
     CDS             complement(58..456)
                     /locus_tag="U4B05_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN2052067.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(453..689)
                     /locus_tag="U4B05_002990"
     CDS             complement(453..689)
                     /locus_tag="U4B05_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2052068.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(849..1133)
                     /locus_tag="U4B05_002991"
     CDS             complement(849..1133)
                     /locus_tag="U4B05_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052069.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          389 a          225 c          190 g          446 t
ORIGIN      
        1 tcagcatatt ccttaatgac atgctgatct aattgagaaa taaagttttt aattcgttca
       61 ttttattgcg gtttgtcgta aataactggt tatcttggac tttagtgaat cgaaatccaa
      121 tgaactagtg gtaatcatta tgataaaatc aacagcttct tgacgagaaa aagcaactgg
      181 acaattattc aattgaagaa acaacaacat ggcaacaaga gcagttcgtt tattgccatt
      241 atgaaaagga tgttttttag ctaaattaat tgctaaaatc gccgcttttt catagatttc
      301 aggatagagt tcttgattga atactgcttg tgaaggctgt ttgacggcca tatctaatgc
      361 atttgcatct tttacaccga tcatttcgcc tggcgagtga cgtaaaataa ctttcgcgtt
      421 gattttgata atttctttag tcgaaagata cctcataaat caaccaaatc ttttaacgcg
      481 tcttcatatt gattcatgat cgaatcaact aatgaatcaa tatcaggttc ttcttgtgct
      541 ttgatcattc gtacagaacc atcagattga aaaacataac tgattgcgtc acctgtctgc
      601 acacccatga attcttttac ttctggtggg atagttgtta catcgctatt gccagatttt
      661 ctaattttat actttttcat ttttttcata tttcgccacc ctctatacca ttctcattct
      721 atagtaagta cattatatcc aagtatatac ttttaatcaa tgtatatact tttattttgc
      781 tttttcttac gaaaaaatga caaagaaaaa agtttgtgac atgacttttg tcacaaactc
      841 tcgtattctt aaccaaaata ttgatgaaat acttgtgtaa taactggtag aaaataaata
      901 atgatcatag ccgctaccac caccaacaat tgttttcgct gtttctttcg ctttagcatg
      961 cttgtcgttt tatgaaaaaa ataagtagcg cttttagctc ctgcagtcaa agtcgataaa
     1021 atcaacgttg gtgaatatgc tgtttgattg tgcgtcaatg acaacaactt cgtcccgtaa
     1081 tcaggatact ttagaaatgc ttggtacaca gccacaatcg tgattgctcc cataagataa
     1141 tttaaaaatg aatgcacttt tttcaaataa aaaaactcct tgctatctat ataaaatcag
     1201 cctaccccaa cgtttttgct tgattcactg atatttttta gttttattgg
//
LOCUS       DASJST010000294         1249 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.301, whole genome shotgun sequence.
ACCESSION   DASJST010000294 DASJST010000000
VERSION     DASJST010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.301"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..165)
                     /locus_tag="U4B05_002992"
     CDS             complement(<1..165)
                     /locus_tag="U4B05_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2052070.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETI"
     gene            278..1030
                     /gene="xth"
                     /locus_tag="U4B05_002993"
     CDS             278..1030
                     /gene="xth"
                     /locus_tag="U4B05_002993"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN2052071.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          392 a          214 c          238 g          405 t
ORIGIN      
        1 gatcgtttca tatagatgtt ggaacccgta ctctttttga attctttctt ttgtttcttt
       61 atttcttgtt gcaaaataaa aatcaagtgt tccttgtttt ttcgcataag ttggtaaata
      121 tgctttttct gcgatattcc ctacaccgat tacaccaatc ttcatctact ttcctcctgg
      181 atcatttagt tactttcatc ttacaccatt ttttactaaa atgaggtaca atagtatcgc
      241 aaaggtaagg aaattaattc gaaggagtgt gcttttattg aaatttatct cttggaacgt
      301 aaacgggctt cgtgccatcg taaataaaaa ttttttagaa gtttttcatg agttagacgc
      361 cgattttttc tgtctgcagg aaacgaaact ccaagcagga caaattgatt tagatcttcc
      421 aggttattat caatattgga attacgcgga acgaaaaggc tattccggca cagcgatttt
      481 tgctaaaaaa ccagcattga atgccactta cggaatgggt atcgatatcc atgatacaga
      541 aggccgtctg attactttgg aatattccga tttctttctt gtaacctgtt acacgccaaa
      601 ttctcaaagc gaattaaaac gtttagatta ccgattagaa tgggaagaag ccttttataa
      661 ttatttggaa aatctaaaaa aacaaaaacc agtcatcgtg tgtggggatc taaatgtcgc
      721 tcatcaaaag attgatttga aaaactggaa aacgaatcaa aaaaatgctg gattcactcc
      781 agaagaaaga gcggctcttt ctcgactgtt ggataacggg tttatcgata cattccgtta
      841 tttttatcca acccaggaag gtgtctactc ttggtggaac taccgattca attcaaggaa
      901 aaacaatgct ggttggcgga tcgattactt tttgacgagc aaagatttag agccaggact
      961 agctgatgca aagatccata cggacattat gggcagtgat cattgtccag tagaattaga
     1021 tttgaaataa agaggttgtg aaaaagtcgt ttagtttcga gtattaagaa aaattttgtg
     1081 aaaatggctt tccacatttt gcataaattt ggcataatac cgagaaactg gcttttgaac
     1141 actgtttatc gagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
     1201 aaaaatagtt cctcatattt ttcgtttggt tgggcttatt accgcagat
//
LOCUS       DASJST010000295         1230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.302, whole genome shotgun sequence.
ACCESSION   DASJST010000295 DASJST010000000
VERSION     DASJST010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.302"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..367
                     /locus_tag="U4B05_002994"
     CDS             11..367
                     /locus_tag="U4B05_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2052072.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            357..623
                     /locus_tag="U4B05_002995"
     CDS             357..623
                     /locus_tag="U4B05_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2052073.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            782..1060
                     /locus_tag="U4B05_002996"
     CDS             782..1060
                     /locus_tag="U4B05_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052074.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(1057..>1230)
                     /locus_tag="U4B05_002997"
     CDS             complement(1057..>1230)
                     /locus_tag="U4B05_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2052075.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT          472 a          168 c          246 g          344 t
ORIGIN      
        1 ataattgggt atggaaaaaa tatcttttga aacatttaaa agacccaatg gtcatgatga
       61 atttttagaa tggttggaaa cattaccaaa aaaagatagt gcgaagcttt tacgtacaat
      121 agaagaaaca gaaaagaatg ggatgctagt agctcaacgg ttgaaatggg tgaaaaaatt
      181 agacactaat ttgtacgaat taagaagtaa ggtaggatca aacatccaaa gagccatcta
      241 tttccatgtg gacaatggaa ggtatgtcat tactcacgga tttactaaaa aaacggagaa
      301 aacaccaata actgaactca agcatgcaaa agaacttaga gaggagtggt tcacaaatga
      361 tgattaaaaa attgattgaa gaaaagaaaa aagataaaga ttttgcagaa gcatatgacc
      421 gtgaaggaag aaaactcgaa actgcggttg ctctttatca tgctcgggag gaagcagggc
      481 taactcaagc ggagcttgca caaaaagcac atacaactca atctactatt gctcgaattg
      541 agagagggga taacgtatct ttcgataaac ttgatgatat cgccagggca ttaggtaaaa
      601 agctggtagt agaatttgct taaaagcgag ttgaaaatat atctataata ggctcctaac
      661 taatcaatta gtttagtggg ccttttttta ctagatagat aaaggttgta ctatagtgta
      721 aaagacaata aattttatac taaaattact ttaaagagaa ctagagagaa aggtacaata
      781 aatgaaaacc ataaatctta taaaagtatt tatagcatca ccaggtgata tagcagcaaa
      841 aaggaatgaa gtagaagaaa ccatatatag atggaatata gaaaatacag ataatacaaa
      901 agtgattttg atgcctatac gatgggaaac taatgcgatt cctacttatg taaataatag
      961 ctctgggcaa agtgttatta ataaacaaat tgtagaaaat agggttctgc tgcaaagttt
     1021 taaaaataaa atcagttttt gtattatttc ttcattttaa gctggaattc ccaataatat
     1081 cctgatttca gtgcagactg aaaagccgaa gagagtgcct tcttttcggc ttttcttgta
     1141 gattcctcga atggtttcca tccctttaat tgtggtggaa gctgtacgta gactttgata
     1201 caatttatgt cgacgtttta tcgatcgatg
//
LOCUS       DASJST010000296         1218 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.303, whole genome shotgun sequence.
ACCESSION   DASJST010000296 DASJST010000000
VERSION     DASJST010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.303"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(3..1157)
                     /locus_tag="U4B05_002998"
     CDS             complement(3..1157)
                     /locus_tag="U4B05_002998"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN2052076.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          344 a          260 c          223 g          391 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaa
//
LOCUS       DASJST010000297         1215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.304, whole genome shotgun sequence.
ACCESSION   DASJST010000297 DASJST010000000
VERSION     DASJST010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.304"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..947
                     /locus_tag="U4B05_002999"
     CDS             414..947
                     /locus_tag="U4B05_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN2052077.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DASJST010000298         1195 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.305, whole genome shotgun sequence.
ACCESSION   DASJST010000298 DASJST010000000
VERSION     DASJST010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.305"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="U4B05_003000"
     CDS             <1..1076
                     /locus_tag="U4B05_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2052078.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DASJST010000299         1193 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.306, whole genome shotgun sequence.
ACCESSION   DASJST010000299 DASJST010000000
VERSION     DASJST010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.306"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..266)
                     /locus_tag="U4B05_003001"
     CDS             complement(<1..266)
                     /locus_tag="U4B05_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2052079.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKI"
     gene            complement(267..1175)
                     /locus_tag="U4B05_003002"
     CDS             complement(267..1175)
                     /locus_tag="U4B05_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEN2052080.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
BASE COUNT          391 a          214 c          127 g          461 t
ORIGIN      
        1 tcaattttcc ctacagtatc tgtagctaat tcaggtttac gttctacaat tttcacagac
       61 ggatttaact tagtcgtcaa ttctactaac tcattactgt ccgtattcaa acaagtcgtg
      121 caattatgtt caggatgttt ttgttgatag atatctatca ttgctaaagc gaaatggact
      181 aggggctttc ctgccaaata tttcaaattt ttatttttaa accccttaga cccagctcta
      241 ccacaaatag taaataaaat ttccatttat ttctcctcag ctattttcaa tacttcgttt
      301 gcatgatata aatcattcca gttttctttg cctgttacta cgttttcaaa gaaatatttc
      361 aactccttct catacatatc attagcagat tcaggcattt ctttaacaat gttttcttca
      421 aaacttattt tattatttaa taagtcaaca ttgatacatt catctaaagt aaacaattga
      481 aattttcttg taggaactct tccaaaataa tctaaatgaa tttctataat tttatcatcg
      541 tatctcaata tatatgttgc taaatcatta ctgttaattt caagattcga atatttacca
      601 aactgttttt ttactgattt agggaaacca aataggttta taatataatc caattcatgt
      661 atacagtcta attctactcc tccccccata gatttaaagg cagaataatt atttctataa
      721 tcagtacctt ttctccaatc aggaagataa gtagaacaaa tagctcttac agaatatact
      781 tttttacttt gaatactatt acgaacaact tccatgatat ttttatacct cagtggagca
      841 gcaacataaa tatttttgta atcataatta tattgagtaa gatcaatact ctcagaaaaa
      901 gcaggttttt ctaaaaatat gtaatcaact ttttcaacaa ttacatttag tgtttccaga
      961 tgcttaaaag tcggatttgt aataaaagct atatcataat gtgtatccaa atcgtctcta
     1021 ttttgaatat acgttatttc ttcaaactct ttttttccac tgctactata agctgttata
     1081 tcaaaattaa tagaattctt ttttccatag cttattagat tttttagatg tctctttcca
     1141 attgacccta acccaaaaaa aattactttc atcattaatt tacctcaatg tcg
//
LOCUS       DASJST010000300         1186 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.307, whole genome shotgun sequence.
ACCESSION   DASJST010000300 DASJST010000000
VERSION     DASJST010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.307"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1072)
                     /locus_tag="U4B05_003003"
     CDS             complement(<1..1072)
                     /locus_tag="U4B05_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2052081.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSF"
BASE COUNT          340 a          259 c          189 g          398 t
ORIGIN      
        1 taaagcttga ccatggtttc aaaaagtcaa ggaggaataa ttcgtcatcg gagattcttc
       61 ctacaacatt gacacgtccg tcgtttttca tatccttcaa ggcgatttgt gtttctcctt
      121 tgtattgacc gtacccttca ttgtcaatca gtacatcccc atgatgcata tctcgcgtat
      181 tatgaggcgg gaaatcttta tctttgtagt atacacgagt catcgttgaa cgaaggatat
      241 attctgagcg atcccctcga taactatgaa ggttatcaaa caagcagatt cgttcgtttt
      301 ctgtaatacc ttcttccgta tctactttca atgttggata atccgcgtta aatgcttctg
      361 ccatttcttt caattcttct tcacttgcgt acgcatttcc aatagaaata tcatcaatca
      421 atcctgtaag gattaaatgc ttcacttgtg tcgcaatttc aagatctcgg tggtcttcca
      481 gcgtgcacaa gccatcttgc gtaggccaag ggccaaactc agcagattgc gaattgacga
      541 atgccatcgt attcaaatta tattttctga atttttctga acaaaaaaca aagtgttcgt
      601 atcctaaacc agagtatcga tgaggataaa aattatgaga acctaataaa ttatccgtgt
      661 ttggcgaata actcatgatg ttgtcaacgt aattcgtgcc cgaactcata ttgatctcga
      721 ttttgatacc aaaaggattg cgcgtcattt ttgcttcttc tgctcctgtg aatccgatat
      781 ctaagcggac cccgtaggca cccatcttat ggaaaaaaga taagtcatcg taagaaatct
      841 ccaactgttc aaataacgct ggattgatat caaccattac ttccatgcct aggctattcg
      901 cgtaatcaac tacttcttta aattctgaaa gaactttttc tttatcatca ttgatctgca
      961 gcaaactggt aaaaacccgt ttatacccat acttatgagc taaatccaag tacgctttgt
     1021 ccttttcaaa tgttgaccgt tctggataaa tcgagattcc taattttccc atattaccat
     1081 ttcctcctgc atcttttcgt ttttgtatcc acttacatta tagcaaacac aaagtaaatg
     1141 gtctacacct tttctttttc ttttaaaata aatgataaat tccaat
//
LOCUS       DASJST010000301         1166 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.308, whole genome shotgun sequence.
ACCESSION   DASJST010000301 DASJST010000000
VERSION     DASJST010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.308"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..582
                     /locus_tag="U4B05_003004"
                     /pseudo
     CDS             1..582
                     /locus_tag="U4B05_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            640..>1166
                     /locus_tag="U4B05_003005"
     CDS             640..>1166
                     /locus_tag="U4B05_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052082.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFE"
BASE COUNT          383 a          160 c          158 g          465 t
ORIGIN      
        1 tttccagcat ttaaaaacat ggaaatattt ggcttttatg atacggcatt tcacattaat
       61 cgtgcgttaa gtttagaatc tatttttagc tcccctatta attttgaaac gtttagatca
      121 tatggcatgc aagtaaataa tttttaccca tggttaactc tatatccttt atttttattg
      181 atcaagttta ctaatttagc aataggctat aacctttttc tttatattgt tacgctcatt
      241 actctattta tatgtcatta tgtaatgtat gaaattacca aaaaacatgt tactagctcc
      301 tttttcgcta ttatatatac aacatcttct tttaggagtg tagaaatatt tcttcgaggg
      361 gcaatggggg aattactcgc aatgtcaata cttcctctta ttttactcgg gtttataaag
      421 ctttacgact caaaaaaaga atcttgggtt atgttggcga ttagcatgac tttattaatt
      481 tacactcatg ttctttctgt agctatgact atggtaatga ttataattgc tttaattatt
      541 caattttatc ggaaaaagaa aatagaaatc ttcttaatta ttaaattaat aaaagctgct
      601 atagttactt tacttctttc actagcgttt ataggtccta tgattgagca gacattgtat
      661 caagatctga atagacctta tgtggatata ttacaaaaaa gagcaattta tctgggtaaa
      721 gagtttttga ttggaagtat agattcagaa ttactatcat ttggtattgg cttaactttg
      781 cttatttgtt taattatact aacatttaac tttaaaaaat taggaatctt atctaaaatc
      841 acttatataa tgggtataat atctattcta ctttgtacaa aaatatttcc atggtttgcc
      901 ttacaaaata ctcctttaaa tattattcaa gctccttggc gttttatgat attttcaaca
      961 ttgttcatat ctttttcagt atctctattt atttctccac agttggaaaa attttcttat
     1021 acaaggagaa gaaattttat actattatta atattagcta tttcaatttt aaattattca
     1081 tcagtagtta acatggtgaa agaaatgaat tttcaaggaa cttattctaa tgaacaaatg
     1141 gaaaataaac ttgaagaatt tgagaa
//
LOCUS       DASJST010000302         1160 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.309, whole genome shotgun sequence.
ACCESSION   DASJST010000302 DASJST010000000
VERSION     DASJST010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.309"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..227
                     /locus_tag="U4B05_003006"
     CDS             <1..227
                     /locus_tag="U4B05_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEN2052083.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="U4B05_003007"
     CDS             227..574
                     /locus_tag="U4B05_003007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052084.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..>1160
                     /locus_tag="U4B05_003008"
     CDS             571..>1160
                     /locus_tag="U4B05_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN2052085.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYD"
BASE COUNT          431 a          163 c          223 g          343 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca
//
LOCUS       DASJST010000303         1158 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.310, whole genome shotgun sequence.
ACCESSION   DASJST010000303 DASJST010000000
VERSION     DASJST010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.310"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            16..276
                     /locus_tag="U4B05_003009"
     CDS             16..276
                     /locus_tag="U4B05_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052086.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(638..877)
                     /locus_tag="U4B05_003010"
     CDS             complement(638..877)
                     /locus_tag="U4B05_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052087.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          408 a          163 c          216 g          371 t
ORIGIN      
        1 aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata tatttgccaa
       61 cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga aaattgtgtt
      121 gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga
      181 aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata tctatataac
      241 tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag tgccttcaaa
      301 agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg ttcatgattt
      361 ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc tggtggagaa
      421 actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa actagtagtt
      481 ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc aggatcattt
      541 agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg agtaaacgcg
      601 aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt tgtttgaaat
      661 aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa acttttatat
      721 cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg atactattcg
      781 aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca gtaagtaaca
      841 ataaatttga taaactgata aacagaattc ttttcattat gtacacactc cttatagtat
      901 cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt
      961 tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc agttcaaacc
     1021 agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt tttatgaaaa
     1081 aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt gggtatttaa
     1141 atagttgcta ggaggata
//
LOCUS       DASJST010000304         1148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.311, whole genome shotgun sequence.
ACCESSION   DASJST010000304 DASJST010000000
VERSION     DASJST010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.311"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(46..1023)
                     /gene="guaC"
                     /locus_tag="U4B05_003011"
     CDS             complement(46..1023)
                     /gene="guaC"
                     /locus_tag="U4B05_003011"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN2052088.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT          325 a          254 c          184 g          385 t
ORIGIN      
        1 atgcaggaca actttttcac aacatcctaa tattttattt attttttaga ttgtgtctcc
       61 attaaaaatc gaatttttca cgatgacata gtcaactttt cgaatggctt ccagatctct
      121 tcctccagca taagaaatag atgattgaag gtcttgttgc atttccacta gtgtatcttt
      181 caaagatcct ttgtgttgga tccagatttt ctttccttca acatttcgtt tttcgccttt
      241 ttggaattct gaagcagaac caaagtattc tttatacacg attccatttt cgacttttgt
      301 ttcgccagga gattcctcgt gaccggcaaa caatgaacca atcatgacca ttgtcgcacc
      361 aaaacggaca gattttgcaa tatcgccatg tgttctgatc ccaccgtcag cgatgatcgg
      421 cttgcgagct gctttcgcac accatctaag agctgccagt tgccagcctc cagtcccaaa
      481 gcctgtcttg atttttgtaa tacatacttt tcctggacca atcccgactt ttgttgcatc
      541 tgcgcccgca ttttccaatt cacggacggc ttccggtgta ccaacattac cggcaatcac
      601 aaaagtttca ggcaaatatt ttttaatatg ctgaatcata tcaataactg agttagcatg
      661 tccatgcgcg atgtcgatag tgatataatc aggatttagc gattcttctg ccaacttctc
      721 tataaaggaa tattcctctt tcttgacgcc aacactgatc gaggtaatca atccacgctt
      781 tttcattttt ttgataaacg gaattcgagc tgcttcatca aaccgatgca tgatatagaa
      841 ataaccattt tccgctaaga attctgcgat tgtttcatca ataatcgtct gcatgttggc
      901 aggcactact ggcattttga atgtatgttt acctaatgtg accgttgtat cacattcaga
      961 acggctattt acgatacatt tattaggaat taattgaata tcttcgtaat caaatacttt
     1021 cattacatta tcctcctgat gttcacatcc agcaaaacac gaacattatt aatttaatca
     1081 aggctttttt tagcgcccta tgtaatttaa ctcaattaag caaaaaaatc aagagcaaaa
     1141 agagaatt
//
LOCUS       DASJST010000305         1146 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.312, whole genome shotgun sequence.
ACCESSION   DASJST010000305 DASJST010000000
VERSION     DASJST010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.312"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            139..>1146
                     /locus_tag="U4B05_003012"
     CDS             139..>1146
                     /locus_tag="U4B05_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2052089.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT          469 a          179 c          204 g          294 t
ORIGIN      
        1 aaagtttgcg tatttcctag gaatgtgatg aaattttgaa ttttgtttga aactaccacg
       61 acaacgtttt taaaggaaat gggatatggt atagttaata tataaaaata ataactaatt
      121 acaggagtgc aaattttaat gaaaaaaaaa tcaattattt tggcagctac tagtgcgttg
      181 gctgttttaa ctctagcagc ttgttcaggc gatactaata aagatatcgc aacgatgaaa
      241 ggcggaacga tcactgtatc tgatttctat gatgaagcaa aacttgaatc atcgaaccaa
      301 tccttagtac aacggatgat catttataaa gtattcaata acaaatatgg cgataaagta
      361 accgataagc aagtggatgc agaatacgac aaacaagcga aatcattagg tgatactttt
      421 gaatcacaat tagaagctgc tggatatacg aaagatactt ataaagaata tattcgtaac
      481 aatttagctt ttgaagctgg cttgaaagct cacgtagata ttacagatga cgatttgaaa
      541 actgcttgga aatctttcca tccagaagta gaagcacaaa tcatcaaatt atcaagtgaa
      601 gatgaagcaa aagatgtgaa aaaatcagcg gatgatggag atgatttctc taaattagcc
      661 aaagacaaat caactgatac agagacaaaa gaagatggcg gtaaagttaa attcgactca
      721 acgacaacaa ctattcctgc agaagtaaaa gaagcagctt tcaaattgaa agacggtgag
      781 atctccgatg ttattactac aacaaatcct acttcttatg cgacagaata ctacgttgtc
      841 aaaatggtga aaaaccaaaa taaaggcaat gacatggata aatacaaaga tcaactaaaa
      901 gatatcgcta ctgaaactaa actaagtgac aatgcattca caactaaagt aatcggtgaa
      961 gaattaaaag atgcaaatgt aaaaatcaaa gatgatgcct ttgaaaatgt gttgagtgca
     1021 ttcacaacaa cttcatcaag tacgaaagac tcaagtgaaa ctacagcttc aacaaagagt
     1081 tctgacacaa aatcaactga ttcaacaaaa gaatcttcaa ctgaagaaac aactgattca
     1141 tcaaaa
//
LOCUS       DASJST010000306         1105 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.313, whole genome shotgun sequence.
ACCESSION   DASJST010000306 DASJST010000000
VERSION     DASJST010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.313"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1087
                     /locus_tag="U4B05_003013"
     CDS             <1..1087
                     /locus_tag="U4B05_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2052090.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DASJST010000307         1073 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.314, whole genome shotgun sequence.
ACCESSION   DASJST010000307 DASJST010000000
VERSION     DASJST010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.314"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1073
                     /locus_tag="U4B05_003014"
     rRNA            <1..>1073
                     /locus_tag="U4B05_003014"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DASJST010000308         1072 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.315, whole genome shotgun sequence.
ACCESSION   DASJST010000308 DASJST010000000
VERSION     DASJST010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.315"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..247)
                     /locus_tag="U4B05_003015"
     CDS             complement(86..247)
                     /locus_tag="U4B05_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEN2052091.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(571..903)
                     /locus_tag="U4B05_003016"
     CDS             complement(571..903)
                     /locus_tag="U4B05_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052092.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
BASE COUNT          321 a          221 c          134 g          396 t
ORIGIN      
        1 acagcgacaa taatgacgtc attttttgaa ttgtttgcct ttaaaatgat tcattactct
       61 atcctctctg tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa
      121 cagaaccact tttcgtaata tcaatgtctt tattcgtatg atttttcgtt ttacgatcta
      181 catgattttg aagtccattt aacgacccaa ctttatgctt ttgtgttcgt gccaccatat
      241 aggccataac tgtgtacttc ctttcgttaa aattattgcg agaatcacaa gctaaattcg
      301 gaaaaacctc catgtaaggt ataacttact agacttatac tgcgtaaagt ctgtaagctc
      361 tgccgagggc tatatcggct taacagccga tgagaaactc cgtttctctc aaacgaaaaa
      421 gcgaacgaaa aacgactgcc ttcgcattcc ttttttacgt ccttttttcg ttcaaaacca
      481 ttggcttttt gcgtcattgt tttcacaatt tttattccgc aaaaaaccaa aagtgaaaag
      541 tattgctttt cacttcaacc cctcctcaat ttagttcaca ttttctgaac gaaattgtgc
      601 cataaattga gtcaaaccct taacatcatt cgtacgccaa tatgcaccat tttcttgcca
      661 aactaatcca tctgcaaatt ttcttagcga tactaaaacc tcatcattta ctgaatgatt
      721 tcttttattt gattccaaaa aatctaatgc ttcagcaggt gtcttaaagc cttccttttt
      781 acaatataat ttcgctaaag aaatcatttc tttctgtcta atctgttttc gtttcgctct
      841 aatcttcgct tcttttgctt tcaaatccgc catttctttt gccaatattt ctctattgtt
      901 cattttgatt cctcctaaaa ataaggcata tttctagttt aaaatattta ttttacgaat
      961 agaggatttg aacaacgaat gacacaaaaa caacaaaaaa agagcaatca tcataagatg
     1021 attgctctaa aaaactgtag tatttttggc caaattttta attttttcaa tc
//
LOCUS       DASJST010000309         1070 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.316, whole genome shotgun sequence.
ACCESSION   DASJST010000309 DASJST010000000
VERSION     DASJST010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.316"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          366 a          181 c          154 g          369 t
ORIGIN      
        1 taattcttct ttatttttat atgttctcat tatattacct ccataaattc ctaattgtct
       61 cctttaattc atctataatt tcatccacga tattttaatc cgttctacaa tgcatatctg
      121 atatgcattg actgtctaac tcatatgtga atgatttata aaatatccca acgtctcatc
      181 taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg aatatatttc ttttgtagta
      241 aaatcggact ggtttgcata tcattttctt cgtaaatatc aaaatcatgt taactactat
      301 caattactaa taaaaactac cactttaagc gttaagaact gttaagttta gttaagtgag
      361 gatagttcac aaaatccatt gttaaacttc acgaaagtgg taaaactcaa aatgctttat
      421 ataggattac agcatcgctc tttcttcgat tgcgcgttgg gtaaaaaata ttatgaagta
      481 aaaattgacg atgacacgtt ccttacggct catcagatga aacagctaca aaaacgaaat
      541 gatcaactcg aagaaaattt aattctaaaa aagcgattgc catattcacg cctcgctcga
      601 cgaactatta aaggtcaaga tttacctgaa gatcatcctt tttaggaaat tgaagctttt
      661 tatgttgcac aatgcattta taatactaca atgatgtttt caccagatat tcttctaggg
      721 tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc accgcgaatt tactaaaatt
      781 atgaataatt atgttaatct tccgaaattt ttttccaaaa ttaagggatc aagctggtgt
      841 tatagattgt ttaaggttgg caaaaaaata acttcttttt aaacaattat agaaaaccga
      901 tactctgtta agttggttga cacggtgtcg atttttatga ctttcttatt ctgaaacttc
      961 actaacggac agtacctaaa aagtctatag tatggacgtt tacgagtact attttatcaa
     1021 aaatgctgct catttcaact tatatgtttt agtgaactac ccccacttag
//
LOCUS       DASJST010000310         1061 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.317, whole genome shotgun sequence.
ACCESSION   DASJST010000310 DASJST010000000
VERSION     DASJST010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.317"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..992)
                     /locus_tag="U4B05_003017"
     CDS             complement(36..992)
                     /locus_tag="U4B05_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052093.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DASJST010000311         1045 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.318, whole genome shotgun sequence.
ACCESSION   DASJST010000311 DASJST010000000
VERSION     DASJST010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.318"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="U4B05_003018"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="U4B05_003018"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN2052094.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DASJST010000312         1035 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.319, whole genome shotgun sequence.
ACCESSION   DASJST010000312 DASJST010000000
VERSION     DASJST010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.319"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="U4B05_003019"
     rRNA            complement(<1..>1035)
                     /locus_tag="U4B05_003019"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DASJST010000313         1027 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.320, whole genome shotgun sequence.
ACCESSION   DASJST010000313 DASJST010000000
VERSION     DASJST010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.320"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..946)
                     /locus_tag="U4B05_003020"
     CDS             complement(<1..946)
                     /locus_tag="U4B05_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2052095.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVL"
BASE COUNT          314 a          212 c          162 g          339 t
ORIGIN      
        1 gcaacactag tgactttagg agaaacatca atagccttgc taaagttttt gctttcgcta
       61 aagagagtat tttcgataat ttgttttttt ctaaaatacc tcgttcctca atatttggga
      121 tatggatatg tatagggatt cttcgcataa aggtttccag aaaatcctct ttgccttctg
      181 tagtcgcaaa aactaaacgt acattggagg cgtgatggat agtttcaccg actctagaat
      241 aagttccgcc atccatataa acaaatagtt tctcttgccc ttcaggaggc aatctgtgta
      301 cttcgtctaa aaacagtact ccaccgtctg catcttgcaa caatccattt cgatcttcat
      361 ctgcgccggt aaaagcacct ttaacatagc caaatagtat ccccgacaat aattcaggat
      421 tattatggta ttgcgcacaa ttaagtactt taaaaggagc attctcatga ataacgttca
      481 cttttttcat aaactgataa gcagtatgaa caaaaaaact ttttccaact ccagtatctc
      541 cagtgatcat cataggaagc cctcttccag gatagaagac agcagttttc atttgttcca
      601 ccgcatcgga taagctcata tcatacccaa tcatcttttg tagaatggaa tctgtagaat
      661 tgggctccaa gtctgttttt aattcagata tactttcgta tacatctttt accggaatca
      721 aaaagtgctc ttcaaagcgc tctttgatgg caaataacac tggacgagta ttgattttaa
      781 atagaacgtt ctttgcaact aagtcattgg caagtttact ggcatttgtt cttcccgttc
      841 ttaaaaattg cgacaggtca tttgtgtcaa aaaagcactc ttctaaatta ctgatgccaa
      901 tttcttcaca ttttgtgttt gcataagtca ctatatcatt tttcatctta taaacagtcc
      961 tttctaaact tctatccctt aaataaatag caaaaaaaag gaagcaatat cgatatgctg
     1021 tcctctt
//
LOCUS       DASJST010000314         1019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.321, whole genome shotgun sequence.
ACCESSION   DASJST010000314 DASJST010000000
VERSION     DASJST010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.321"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..791
                     /locus_tag="U4B05_003021"
     CDS             207..791
                     /locus_tag="U4B05_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2052096.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          328 a          145 c          199 g          347 t
ORIGIN      
        1 tttaaaaaaa gagtgtctag tcgacattct ttttttctaa ttgaaagtgt tcataaaatc
       61 acccatttct ggacactttc tttttttgct ttatttttca aaaaaacaat tcatttcatg
      121 ttcataaacc cataacaata tctatgttca gttaaactat atggataaag ttatgttaca
      181 ataaaattaa tgaaggagtg atttttttga aaataggata cgcccgggta tctactggtt
      241 tacaaaattt ggatttacaa gaagacagcc taaataaatt tggttgtgaa aaaattttta
      301 ctgatcatat gagtggggca aaaagtaatc gaccaggatt agaaatggcc attgattttg
      361 ttcgctctgg cgatacactt gtcgtttggc gcttagatcg tttaggtaga aatatggaag
      421 atttgatttc aatcgtcaat cgactgaatg agcgtggtgt gagttttcat agtattcaag
      481 aaaatattac gatggacaaa tcaagttcta caggacagct catgtttcat ttgtttgcgg
      541 catttgcaga atttgagcgt aatttgatcc tggaacgatc cgcagcagga cgagcagctg
      601 ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt gtctgaacaa gatttggaat
      661 tactgaagac tttagtggat agcggtacac ctattaagac tattgcggaa cggtggaacg
      721 tctcaagaac caccatttat cgttatttgg caaaaatgga ggagaaaaat ggaaagacaa
      781 attctcatta gtttttgtca tttaatgctg aaaatttttg ccatttatct agaaaagtga
      841 caatagtatg agaatttttt gtaagtgtct actgataact tattctcaaa tgttgtaagg
      901 attttattca tttgttaata ttaaagaacg gaactccaaa aaagtccgta ctctaatttg
      961 cagtctctca ctcttttaga gtgagagttt ttattagatt ttcaaattaa ttagtaaag
//
LOCUS       DASJST010000315         1017 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.322, whole genome shotgun sequence.
ACCESSION   DASJST010000315 DASJST010000000
VERSION     DASJST010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.322"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..120)
                     /locus_tag="U4B05_003022"
     tRNA            complement(47..120)
                     /locus_tag="U4B05_003022"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Ile,seq:cat)
     gene            complement(134..207)
                     /locus_tag="U4B05_003023"
     tRNA            complement(134..207)
                     /locus_tag="U4B05_003023"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Met,seq:cat)
     gene            complement(235..307)
                     /locus_tag="U4B05_003024"
     tRNA            complement(235..307)
                     /locus_tag="U4B05_003024"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Ala,seq:tgc)
     gene            complement(327..400)
                     /locus_tag="U4B05_003025"
     tRNA            complement(327..400)
                     /locus_tag="U4B05_003025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Pro,seq:tgg)
     gene            complement(410..483)
                     /locus_tag="U4B05_003026"
     tRNA            complement(410..483)
                     /locus_tag="U4B05_003026"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(447..449),aa:Arg,seq:acg)
     gene            complement(494..579)
                     /locus_tag="U4B05_003027"
     tRNA            complement(494..579)
                     /locus_tag="U4B05_003027"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(543..545),aa:Leu,seq:taa)
     gene            complement(596..667)
                     /locus_tag="U4B05_003028"
     tRNA            complement(596..667)
                     /locus_tag="U4B05_003028"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(633..635),aa:Gly,seq:gcc)
     gene            complement(679..751)
                     /locus_tag="U4B05_003029"
     tRNA            complement(679..751)
                     /locus_tag="U4B05_003029"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Thr,seq:tgt)
     gene            complement(769..850)
                     /locus_tag="U4B05_003030"
     tRNA            complement(769..850)
                     /locus_tag="U4B05_003030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(814..816),aa:Leu,seq:tag)
     gene            complement(858..930)
                     /locus_tag="U4B05_003031"
     tRNA            complement(858..930)
                     /locus_tag="U4B05_003031"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(895..897),aa:Lys,seq:ttt)
     gene            complement(934..1006)
                     /locus_tag="U4B05_003032"
     tRNA            complement(934..1006)
                     /locus_tag="U4B05_003032"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(971..973),aa:Val,seq:tac)
BASE COUNT          266 a          291 c          241 g          219 t
ORIGIN      
        1 cagccggact tgggtaatcc tccatatgaa acttacaacg ccacaatgga ccttgtagga
       61 ctcgaaccta cgaccggacg gttatgagcc gtctgctcta accaactgag ctaaaggtcc
      121 aggctcttaa aaatcgcggc ggaggggatc gaacccccga cctcccgggt atgaaccgga
      181 cgctctagcc agctgagcta caccgcgaaa aagatattca ttatttaaat gaaatggagc
      241 ctagcgggat cgaaccgctg acctcctgcg tgcaaagcag gcgctctccc agctgagcta
      301 aggccccgtg tttaaattta taattatcgg gaagacagga ttcgaacctg cgaccccttg
      361 gtcccaaacc aagtgctcta ccaagctgag ctacttcccg tttaattaac gcgcccaaga
      421 ggagtcgaac ccctaacctt ttgatccgta gtcaaacact ctatccagtt gagctatggg
      481 cgcatatttg aaatgccgag gaccggaatc gaaccggtac ggtgatcact caccgcagga
      541 ttttaagtcc tgtgcgtctg ccagttccgc caccccggcg cgattgcttt ggcaaagcgg
      601 aaaacggggt tcgaacccgc gacccccacc ttggcaaggt ggtgctctac cactgagcta
      661 tttccgcgtg tttaaagatg ccggctaaag gacttgaacc ctcgaccctc tgattacaaa
      721 tcagatgctc taccaactga gctaagccgg catgtaaaac ttaaattatg cgggtgaagg
      781 gacttgaacc cccacgccgt aaggcgctag atcctaaatc tagtgcgtct gccaattccg
      841 ccacacccgc aaaaatatga gccgtacagg gctcgaacct gtgaccctct gattaaaagt
      901 cagatgctct accaactgag ctaacggctc atatggaggt taacgggatc gaaccgctga
      961 ccctctgctt gtaaggcaga tgctctccca gctgagctaa acctccatgg ataactt
//
LOCUS       DASJST010000316         1013 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.323, whole genome shotgun sequence.
ACCESSION   DASJST010000316 DASJST010000000
VERSION     DASJST010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.323"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            580..>1013
                     /locus_tag="U4B05_003033"
     CDS             580..>1013
                     /locus_tag="U4B05_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052097.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYG"
BASE COUNT          361 a           96 c          168 g          388 t
ORIGIN      
        1 aaaaaaatat tataaagaat aatattttct aaaaagtaaa ctatagtaat gtggattatc
       61 accttccaac agtatataaa atgagcgtaa aaacggctat ttttgcctta acaacgattt
      121 ttttgtatac aaatacgata tgctcattaa ttattggaat ttgtagtgct gtttaaatta
      181 atgaaggtgt aattatatga gtgatcattt tgaaaaagtt tgttgagaag atgtgcttct
      241 agtcatgtga tgggagaaaa taatttaact gttagagtga atagttaaat tattctggtt
      301 atatgaaaaa agtagtttct aaaataatct tgcagggatt aacttaaaga aattgagaga
      361 atagtttctt tgaaatttct atatacaatg agaagctgaa atttactttt atttgatgac
      421 tggaaataga aattaaatat attgtattgt tacattaaag aaaacttgta tggaaaaatc
      481 agtacaatac cattatttaa tgttcttcac ttaaagcgca aaaaatatca gtttattaaa
      541 ctcgtatata gaacaaatat atgtagacgg ggcgaaacga tgagtgtaaa aaaaatgcta
      601 tttatggtaa gtacaataat tgtattaaca tcccgatttt ggggaattaa tactagttta
      661 tctatgcgat attttattat ggcaatatgg gatttatatt ttatgttaag ctatttttct
      721 tatcgtgaga aaacatataa aaaatacgtt atcaagaata acttttggtt gactataatg
      781 ccgtttgtga tattttctat ctatactcta ataatatggg gaattggttc aaatgcagaa
      841 tttgaaaatt ttataaagtt atgttccacg ttgctttatt taattcttgc atatggttac
      901 gcatgtacag cattttactt atttaaggca gaaggaattg attttatttt ttggtcaggt
      961 gttatttcat atatgagtgg ttcagttttt tatttaattg tatcttatgg ttt
//
LOCUS       DASJST010000317         1007 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.324, whole genome shotgun sequence.
ACCESSION   DASJST010000317 DASJST010000000
VERSION     DASJST010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.324"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(390..476)
                     /locus_tag="U4B05_003034"
                     /pseudo
     CDS             complement(390..476)
                     /locus_tag="U4B05_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          356 a          151 c          129 g          371 t
ORIGIN      
        1 atatgtgctt ttgttagaaa aatcaataca taaattatca tatctgtata tgtcatttag
       61 gtatttcagt tatgttataa tagttttgct gtctgctacc ccggcctaat ctggcaaggg
      121 gaggaggaga tataatgaaa tacctttttg attttgtttt tgcaatcaag ccatttgcag
      181 aggtaactgc acactacatc tgcaagtggc ttgattgcaa aattgccaaa aaagacagta
      241 agcctaccag ttaattctat aatatagaaa cgtaaaaaaa accccatcag cctacacgct
      301 gatggggttt gatgatataa tgaaatacct ctttgttact cataattata ccataattag
      361 tcacgaatgc aaccaataga aaaaactact tagaacggta gatcatcgct aataacaatg
      421 cattgtccac caaatggatt ttgtgaagca ttaaatacat tgttattaat tgtcgctgtt
      481 tttaactagg cctctcccct tctattataa aagaagatat atattatttc ttaattacct
      541 actaccttaa accaaaattt aagtactacc taatttaagt agtacttaaa taatctaaaa
      601 aatagttatc ccaattgtta tgcttttata atacgattat ttgtaacaaa atgaatatta
      661 tataattaaa cactaccata tttaggtagt gtttaattat tagcaaaaca aaaaatattt
      721 attctattct cttaacttca actaagttat gctgcacacc tttatataaa tcatatgcaa
      781 ctcattagct agacaaacta caagcatgta gttttatctg ttaattatgt actacctaaa
      841 taaagtagtt tataattaac aggtatatat atttaaaaat ttaagattaa tttttttatg
      901 acttgtacaa atttatctga tattctctta ttaagaacta cctaatttgg gtagttctta
      961 ttttttacat tttaagtagt atttaattgc aatttggtag ttttttt
//
LOCUS       DASJST010000318         1005 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.325, whole genome shotgun sequence.
ACCESSION   DASJST010000318 DASJST010000000
VERSION     DASJST010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.325"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..788)
                     /locus_tag="U4B05_003035"
     CDS             complement(54..788)
                     /locus_tag="U4B05_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2052098.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          352 a          208 c          182 g          263 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaa
//
LOCUS       DASJST010000319         1001 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.326, whole genome shotgun sequence.
ACCESSION   DASJST010000319 DASJST010000000
VERSION     DASJST010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.326"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            370..1001
                     /locus_tag="U4B05_003036"
                     /pseudo
     CDS             370..1001
                     /locus_tag="U4B05_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DASJST010000320          998 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.327, whole genome shotgun sequence.
ACCESSION   DASJST010000320 DASJST010000000
VERSION     DASJST010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.327"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(80..634)
                     /locus_tag="U4B05_003037"
     CDS             complement(80..634)
                     /locus_tag="U4B05_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2052099.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DASJST010000321          987 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.329, whole genome shotgun sequence.
ACCESSION   DASJST010000321 DASJST010000000
VERSION     DASJST010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.329"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..>987
                     /locus_tag="U4B05_003038"
     CDS             19..>987
                     /locus_tag="U4B05_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2052100.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLEC"
BASE COUNT          394 a          100 c          201 g          292 t
ORIGIN      
        1 ttagaggtat aaaaagttat gaaacacaag gtaagtgtga tagtaccaat ttacaatgtt
       61 gaaaaatatt taagaaaatg tgtggatagt ttattgagtc agacattaaa agagatagaa
      121 atcgtattag ttgatgatgg ttcgccagat gcttcggggg aaatagcaga tgagtatcaa
      181 aaaaaatatt ctaatgtaaa aacaatacat agagaaaatg gggggttagg accggcaaga
      241 aatacaggaa ttgagaatgc gacaggagaa tatgtggctt ttcttgattc agatgattgg
      301 gtagaaagtg atatgtatga aaaattatac ttagttgcgt ccaaagaaaa tgctgatata
      361 gtagttagtg gacattgtga ttttgcgaat gacaaagcta tggtgaaaaa gcaacatcct
      421 ttgagtggga atgtatatga tacgcctaat agtattaaaa atgtacgaaa aaatttattt
      481 ggacatagtc caaaagatca agaagtagag gccttcccga tgtcagtatg tatgtctttg
      541 tataataaaa atctgctaga taaatataga ctaagatttg aaaaaatttt atctgaagat
      601 acaattttta acttgaatgc atatgatgtc gcaaataaaa ttgcttttac agaatataca
      661 gattattgtt atagaaaaga agaacaagag tctattacaa aaagtttatc tccaaataag
      721 cagacggaat ttaaagaatt tttaataact ctaaaagcta tagcatcgaa agaagattta
      781 gaatgtcaaa tcagagcaaa acgtatggca attgattatt gtaggcttta tgtgggctta
      841 gttgatgatg caaatttggg aattaaagag aaaagaaagt atataaaaaa atttattgaa
      901 gatgagggat taattcaatg ttggaaggga tatcctacaa atacgctacc atttcaacaa
      961 aaattttttc ataaaatgct tgaatgt
//
LOCUS       DASJST010000322          981 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.330, whole genome shotgun sequence.
ACCESSION   DASJST010000322 DASJST010000000
VERSION     DASJST010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.330"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..588
                     /locus_tag="U4B05_003039"
     CDS             37..588
                     /locus_tag="U4B05_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HEN2052101.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          376 a          136 c          187 g          282 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa a
//
LOCUS       DASJST010000323          977 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.331, whole genome shotgun sequence.
ACCESSION   DASJST010000323 DASJST010000000
VERSION     DASJST010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.331"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..282)
                     /locus_tag="U4B05_003040"
     CDS             complement(<1..282)
                     /locus_tag="U4B05_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2052102.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
     gene            complement(384..737)
                     /gene="tnpB"
                     /locus_tag="U4B05_003041"
     CDS             complement(384..737)
                     /gene="tnpB"
                     /locus_tag="U4B05_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN2052103.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(727..921)
                     /locus_tag="U4B05_003042"
     CDS             complement(727..921)
                     /locus_tag="U4B05_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052104.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          280 a          198 c          169 g          330 t
ORIGIN      
        1 tttcgttcct acaactcgtt gatgtccttt tactggaata atggtggcag ctgcttctgg
       61 aacacttaca gaaatttctt catcactaaa aagattcaat tgcccattcg tttcctcaaa
      121 gagagtctct ttagagcgtc caaataattt gttggttaaa taattgacct tttcattcaa
      181 tagttggatt tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat tggttttatg
      241 ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc accaccttga
      301 atcgaactga ttttgttaca gaataccaca agaaacgcac aagaaatagg ggttcaacac
      361 attttttgtg ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga
      421 ggttgatcga tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga
      481 acttcgtctt tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga
      541 agaaaaccat caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat
      601 aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga
      661 ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga
      721 agtaaatcat aaagcattgg acaacagttc tttcaaaata gcttgaagaa tataattgtt
      781 aataccgtta tagatcaaaa cttcagaacc attctctctt ttgatgcgca tcgctaactt
      841 ggcagccgcg ggtttacctt tttctggtgc actcttttcg tttcctaatt ggacaggaac
      901 aatctcattt aatttataca tagaaaacct ccagacttct tgatagctca agtatatctt
      961 gaggtgcatt tgttagt
//
LOCUS       DASJST010000324          975 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.332, whole genome shotgun sequence.
ACCESSION   DASJST010000324 DASJST010000000
VERSION     DASJST010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.332"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            95..787
                     /locus_tag="U4B05_003043"
     CDS             95..787
                     /locus_tag="U4B05_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2052105.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          429 a          147 c          161 g          238 t
ORIGIN      
        1 ttaaaattac atatgtaatt taaaaacgct tgacattgtg tttgagatca cttactttta
       61 gcatgtaaaa gtattggatt aaggagctat gcaaatgaaa aagaaattta ttactggtgg
      121 gattgtcgtt gcatttctat gtctaattgg ctacacaggc acgcggtacg caaatgaaat
      181 tattttgtgg ggtggagaga cagatattga tacaatcaat gagaatctga cacagcttga
      241 tcatacattg acagataaag agcaagcgat tacagatctt accgctcgac tagcacaaaa
      301 agaacagaca ttgcaagatt caaaggcaga tgtccaagaa taccaaacaa aagtaaatga
      361 attagaaaac caaaaaaatc aatcggcagc agaaaaacaa aacttagaaa gtcaattagt
      421 cagcaaggat gcacaactac aaagcaaaca aaatgaaatc aatgtcaaac tagatgagat
      481 caaccaaaaa aatgatgaaa tcaatcatct gaatgcagat tggacagcaa gactacaaga
      541 agcacaagca aaactttcag aattacaaaa tactgtaaat ggtttgaatc aacaaatcgc
      601 aacactgaca caagaaaaaa caaatgtaga aactcagtta acagatacac aaaagaaatt
      661 agacgaagca actgctgaaa actcaagctt gaaacaatat attcaaaagt tagaaaaagc
      721 aaaagatgaa gtggaagata cagctgataa atctcagcaa attgttgaag aacatttgcg
      781 taaataatct ttcttaacaa gtaaacctaa attagtaaag aagcaacagt aatctatact
      841 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      901 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      961 taattgaatg aaaat
//
LOCUS       DASJST010000325          970 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.333, whole genome shotgun sequence.
ACCESSION   DASJST010000325 DASJST010000000
VERSION     DASJST010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.333"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>970
                     /locus_tag="U4B05_003044"
     CDS             82..>970
                     /locus_tag="U4B05_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN2052106.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANIN"
BASE COUNT          329 a          182 c          191 g          268 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc
//
LOCUS       DASJST010000326          925 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.334, whole genome shotgun sequence.
ACCESSION   DASJST010000326 DASJST010000000
VERSION     DASJST010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.334"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..764
                     /locus_tag="U4B05_003045"
     CDS             <1..764
                     /locus_tag="U4B05_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052107.1"
                     /translation="ETNTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSL
                     ILIFMGLKRIELLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGI
                     LALLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGG
                     RIAASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFI
                     LYLTDNTLTYPDTQMIFLLVPMIVSWVDNQQRISE"
BASE COUNT          318 a           93 c          163 g          351 t
ORIGIN      
        1 gtgaaacaaa tactgcaaat tttgctatgg aggtacatga tttaactttt gcgatggggg
       61 tttttttcct ttattacgta ttttttgaaa ataaaaatac aaaaaaacat aagataaaaa
      121 ttatcgtatc tttaatttta attttcatgg gattgaagag aattgaactg ttagctttaa
      181 gtggtgcaat tctagtgtat tatgttttat taaagtgggg aaaaaagatt caaatccgtg
      241 cattagtgtg tactatttgt gcaactgtta tttcgttagt gtttatttac attatagata
      301 caggaatttt ggcattgctt atgtcaaatt tgggcattac agatgacggt gcaaggcttt
      361 cattttatct atattcatct aaattttatg aacttggttt aggatatgta ggaacaggat
      421 ggacatggtt ttcaaaatac ttttttaatc tttatttatc gcactttagg ttaaatggtg
      481 gaagaattgc agcaagccta cacagtgata tattaactat ttatatagaa gttggatgta
      541 tagtttttat tatatggatg ttttggttat tttttgtgaa accgataaga ctaactaaaa
      601 aatttggatt gaaaacaggg gagtgtgtat tattattaac tatatatatg tttatattat
      661 atttgacgga taatacgctc acttatccag acactcaaat gatttttctc ttagtaccta
      721 tgatagtttc gtgggttgat aatcaacaac gaatatcgga atagatagct aaagtatatt
      781 ttaattatga atttataatt agttgtaaaa gttaatattt aacggacagg aagtagggtg
      841 aaaaaatttg aaaaaaattt tagtgttagc aaatacatat tatcaaataa tagtagcaat
      901 acaaatgaaa aatacaattt tttcc
//
LOCUS       DASJST010000327          915 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.335, whole genome shotgun sequence.
ACCESSION   DASJST010000327 DASJST010000000
VERSION     DASJST010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.335"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..722)
                     /locus_tag="U4B05_003046"
     CDS             complement(54..722)
                     /locus_tag="U4B05_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2052108.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          350 a          158 c          149 g          258 t
ORIGIN      
        1 tagtttacat aatgatattt ctgtaaatta aataaagtag tgaaaaataa gaatcagtgc
       61 tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat
      121 tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata
      181 gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact
      241 aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca
      301 atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg
      361 caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca
      421 aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg
      481 atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa
      541 ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca
      601 ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat
      661 ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc
      721 atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac
      781 tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa
      841 ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa
      901 aaaacagagt atcaa
//
LOCUS       DASJST010000328          913 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.336, whole genome shotgun sequence.
ACCESSION   DASJST010000328 DASJST010000000
VERSION     DASJST010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.336"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            167..>913
                     /locus_tag="U4B05_003047"
     CDS             167..>913
                     /locus_tag="U4B05_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2052109.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDF"
BASE COUNT          364 a          130 c          159 g          260 t
ORIGIN      
        1 gtgtatataa ttttccagta ataaaaattg atggcgtagt tcgctttgac gtagaggata
       61 tcaaagattt catgaataca ttcaaaaaaa attgaaattt tcttctcgtc acatatacta
      121 taaatagcga aatgtactgc cttacttttt aaaggagtga ggaagtttgc ctacaaagct
      181 ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag aaatcactgg
      241 aattgcatca gagaagtatc aaaaagtatt tccaactagg caattagcta ttaaggccga
      301 aagggaaatg aaaaagaaga ttgaaacagt tttaagagag gagaatgcaa actcactaga
      361 attgaaagga aaaataaaat ttaaagattt ttatgaatca aaatggcttc ctagatatga
      421 actaggtcaa actactaggt caaacagggc tccaagtaat gtaacaatta gtaatactaa
      481 ggatatattc cgattgcaca tccttccaat gtttggtgaa tatgcaatga attatctgaa
      541 ttcaaatact gagataatat cagatgaatt aacaaagaaa tcgaaagaat acgcaaatat
      601 aaaaattatc aaaggctatg ttcgctctat gtttgatatt gccgaaatac tatgttatat
      661 cgaattcaac cgaactacca aaattattca atgtattacc gcgcctaaga aaatggcttt
      721 agaagaaaaa aggataaggg aagggaatca agcattatca tcagaagagc tgactgattg
      781 gatagaagca gtaaacaatg acttaaataa tcacttgctt acacttcacg attacacact
      841 tttcatgctt accttatatc taggtgatag aaaaagtgag acttatgctc tgcaatggaa
      901 atatattgat ttt
//
LOCUS       DASJST010000329          906 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.337, whole genome shotgun sequence.
ACCESSION   DASJST010000329 DASJST010000000
VERSION     DASJST010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.337"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..720
                     /locus_tag="U4B05_003048"
     CDS             <1..720
                     /locus_tag="U4B05_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2052110.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          339 a          143 c          178 g          246 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatatat caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt
      901 cattgg
//
LOCUS       DASJST010000330          890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.338, whole genome shotgun sequence.
ACCESSION   DASJST010000330 DASJST010000000
VERSION     DASJST010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.338"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..190)
                     /locus_tag="U4B05_003049"
     CDS             complement(<1..190)
                     /locus_tag="U4B05_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2052111.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKY"
     gene            complement(187..840)
                     /locus_tag="U4B05_003050"
     CDS             complement(187..840)
                     /locus_tag="U4B05_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2052112.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          288 a          153 c           75 g          374 t
ORIGIN      
        1 tatattttat ttcttttcca ttcatattac tagaaatttc atttgccata ttatgaatct
       61 tttcttttct cgtctccaaa tatgttttag gaaagataaa aaaaattaat gaatgaacta
      121 atataattat tattccaaga acagaaaata tttgtatgaa catttttgga aatatcttta
      181 attttttcat tttctctcca atttatatcc tacatttctt atagtagtta tacagtctaa
      241 ttgtaatttt tttctaagtt cttttatata tacatctatt actcgatcat aaggagtttc
      301 ttcgctatct ttccatacat aatcaataat ttgcattctt gttaacactt gtccattatt
      361 atccaataaa cattttaata cttctagttc ttttgcattt acatctattt cttcaccatt
      421 tattttagct gtatagctat taaaattaac cgatagatcc ttatattcaa acttctctaa
      481 atgtccataa ttcttcttaa ttaaagaatc tattctagct tttaagaccg gcaatgaaaa
      541 tggcttttct acatatccgt ctactaaatt agtaaatgca tcaattttat attcttcatc
      601 actaaatgca gtcaagatta gaattggtaa attgcttttc tttctaattt ctttcaacac
      661 ttctaaacca tttataaaag gtatctgaat gtctaagata accaaattta tatcgctatt
      721 aaattttgac aaggcttctc ttccatcttt agcttgaata acagtatatc caaattctga
      781 aagatattca cttattccct ctcttatcat attatcatct tcaacagtta atattttcat
      841 atatacctcc acttaagcct atacactaga tattatacag catattatgg
//
LOCUS       DASJST010000331          889 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.339, whole genome shotgun sequence.
ACCESSION   DASJST010000331 DASJST010000000
VERSION     DASJST010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.339"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..427
                     /locus_tag="U4B05_003051"
     CDS             <1..427
                     /locus_tag="U4B05_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEN2052113.1"
                     /translation="LLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVG
                     SYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVL
                     PKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            557..>889
                     /locus_tag="U4B05_003052"
     CDS             557..>889
                     /locus_tag="U4B05_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2052114.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          310 a          129 c          168 g          282 t
ORIGIN      
        1 attattattc ttatggatga atgctagaaa gaaagaaata gcagtattac tatcattagg
       61 tatatcaaaa ttagaaattt ttggtcaatt cctaattgag atggtattta tatcaatccc
      121 cgcattcgtt ggttcctatt tcttagctca atatacagct gacaagcttg ggaataatat
      181 attgaataag gttactggtg atatagctaa acaaatagct aaacaatctg catctagcca
      241 attaggtggt ggggcagaag ctgaaggatt taacaagaca ttatcaagtt tagatatcaa
      301 tgtattacct aaattcatga tttatgtagt tatatttatg agtttagtac ttcttgtatc
      361 attgattatt tcttcattca atatattaag aaggaatcca aaagaattat taattgacaa
      421 taattaaaat tttcggtgct ttttagcacc aaaaatgaga gtgtaatata aaccgtgtaa
      481 gtaaccatca atcctaggcg ggtggttgct tcacggtttt tatttttcta aaataagatc
      541 aagaggtgat tacacgatgg ctagaaagaa aagaaatcct gatgctgaga agttagctga
      601 atccattttg aatacttatc aacccgaatc tgtagaggat atgcaagatg ttttaaaaga
      661 tgtgtttggt cccttatttg aaaaaatgct tcaaggagaa ttgaataacc atttagggta
      721 tgacgcccat tctaaagagc caaagaaaca tgacaatcga cgaaacggtt atggcaataa
      781 gacgcttaaa acgagctttg gtaaagtaaa cattgatgtt cccagagacc gggaggcttc
      841 ttttgaacct gagttgattc ctaatagaaa acgagatgtc tccgacatc
//
LOCUS       DASJST010000332          885 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.340, whole genome shotgun sequence.
ACCESSION   DASJST010000332 DASJST010000000
VERSION     DASJST010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.340"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..821)
                     /locus_tag="U4B05_003053"
     CDS             complement(<1..821)
                     /locus_tag="U4B05_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN2052115.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGI"
BASE COUNT          329 a          150 c          111 g          295 t
ORIGIN      
        1 attataccac ttgatacaag tacttgaaga aatccattat gcatgtttcc atgtgctctt
       61 tttagttcat tcacatctgt agtatcaaga tctacttttt tcaaaaattt tgcatcgcta
      121 ttcccataga aatcggtctc tccataacca aataacaaag ttggtcttat aactttaagc
      181 gcagctttcc aaatcgtaaa acgaccagct gaaatttcgg agttatcact agaatgctgt
      241 aatattactt ctttttctgg aagtggagtt ttatctgatt catttgactc tataacttct
      301 acttttctat caatcgatac aggaattgtt gctaaaaaat ccttagcata attagtacca
      361 aaagaaaaat ataccaatag aactaataat atgcttacca tccatttgat ttttacaggt
      421 agtacttctc taatgctctt tctaaatttt gaattggcaa acaacaaaag aaataatact
      481 aaaaaagaac aaattactac ctgggcacct ctagacgaag aaagtgtaat ataaaatatt
      541 tgtacgataa aattcatcat ataaaaaaca cgaatcgttt ttgttatttt tgaaattaat
      601 acactaatta aacttaaaat aatagaagta tatccaaaca tagctccaac atttggactt
      661 gtatagacac caaataaccg atgttcaata aaaccttgtc ttgcaagtaa tcctggcgtt
      721 cctgttggga catgataaga aatcaaaaaa ataaattgat agatagatat tagcgcagct
      781 aaaaaaatta tgataatgaa aacattatta aaatacacca tttttttctt tttttcctct
      841 tcactttgtg aaaagtctgc aggaaaaatt actatcaaag taatc
//
LOCUS       DASJST010000333          854 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.341, whole genome shotgun sequence.
ACCESSION   DASJST010000333 DASJST010000000
VERSION     DASJST010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.341"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>854
                     /locus_tag="U4B05_003054"
     CDS             <1..>854
                     /locus_tag="U4B05_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN2052116.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLS"
BASE COUNT          372 a          110 c          138 g          234 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatc
//
LOCUS       DASJST010000334          832 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.342, whole genome shotgun sequence.
ACCESSION   DASJST010000334 DASJST010000000
VERSION     DASJST010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.342"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            569..>832
                     /locus_tag="U4B05_003055"
     CDS             569..>832
                     /locus_tag="U4B05_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2052117.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFD"
BASE COUNT          264 a          122 c          195 g          251 t
ORIGIN      
        1 agaaaataat tagcagattt acaagtggga aataaaaagc tgaaagaaga aaattgcatt
       61 tcaaactaca ttttctacaa tagctctaaa atggcatatt ttgcgttctg agcggtttta
      121 gcgtttattt cgtttagtta tcggcacaat cggtgaaaca cgttagagga gcgaaaatgg
      181 agcctgtaag gcgactggta gcggaacggt cgtgttagat tgtgtagccg atagctgaac
      241 gaaataaatg ctggcagcgc cattctattt cggcaggagg aggctcaagg ggttcgggga
      301 atgaaattcc ctgaggtctt tttatttttt ctagaggagt gctataatca aagaaaatga
      361 gaaaaaagag taaaaaaata gtcgagcagt gttgacgcac tctcgacctt tacggaaact
      421 acttcaccta gttccgtaac tcaagttcat tatacgaata tttttaggtt tcttcaagct
      481 agagattttt ggatgtggat ttttagagtt acgtgaactc tggaaattct ttccaagaat
      541 ctcttttttc gttgaggagg agctattttt gagtaaaaaa ggcataatcg taaatgagtg
      601 ggaaaaactt aaccgtattt ctaacaggcg gttaagtgtt gacgataagg tacaccccct
      661 aaaattaagt tggtcaatcg atcgaattac aatcgtcgga aaactgaatg ataccatagc
      721 ttattggacg caaaatggag ttgtgtttgt cgattttgag caattaatgc gtttgaatga
      781 aaatcaaggg tatttgaagt cggttggtac aaatggttgg caattatttg ac
//
LOCUS       DASJST010000335          832 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.343, whole genome shotgun sequence.
ACCESSION   DASJST010000335 DASJST010000000
VERSION     DASJST010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.343"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..693
                     /locus_tag="U4B05_003056"
     CDS             <1..693
                     /locus_tag="U4B05_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2052118.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          304 a          132 c          154 g          242 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccacttcaa gcgttaagaa ccgtcaggtt ta
//
LOCUS       DASJST010000336          808 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.344, whole genome shotgun sequence.
ACCESSION   DASJST010000336 DASJST010000000
VERSION     DASJST010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.344"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..159
                     /locus_tag="U4B05_003057"
     CDS             <1..159
                     /locus_tag="U4B05_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2052119.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..>808
                     /locus_tag="U4B05_003058"
     CDS             242..>808
                     /locus_tag="U4B05_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052120.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQT"
BASE COUNT          277 a          104 c          162 g          265 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacc
//
LOCUS       DASJST010000337          806 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.345, whole genome shotgun sequence.
ACCESSION   DASJST010000337 DASJST010000000
VERSION     DASJST010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.345"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U4B05_003059"
     CDS             200..742
                     /locus_tag="U4B05_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052121.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
BASE COUNT          337 a           90 c          147 g          232 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcac
//
LOCUS       DASJST010000338          801 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.346, whole genome shotgun sequence.
ACCESSION   DASJST010000338 DASJST010000000
VERSION     DASJST010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.346"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..702
                     /locus_tag="U4B05_003060"
     CDS             <1..702
                     /locus_tag="U4B05_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2052122.1"
                     /translation="NSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPKR"
BASE COUNT          285 a          147 c          153 g          216 t
ORIGIN      
        1 aacagtcctg tgcagattga gttacaacac acacatgata aagcaacgtt tgagaaaaac
       61 tacaaggcat acgttgaatt ggctagagat tcagcaatga aatatggtat tccattaacg
      121 ttggacactc cttataacca accgggaatc aaatcgcatt tatgggtaac acaaaacatc
      181 tggggcgatc atacagatcc ttacggttat ctttctgaaa tgggcgtaag taaagaaaaa
      241 ttagcatatg atttagctca tggatttacc gatgaaaatc caacaacttc tgaaaacaag
      301 cctgtcattg atccaacacg agctggtgca gctaatccta cactgacaga tggaacgaat
      361 tacgcccaca ttgatcagtt tggagaaatc gaaaatgcaa atttgcatgt agctggatgg
      421 cacattgcta actataaata cgagtatatc ttcattatgg attacaatac tgggaaagaa
      481 ttagctcgag taagagctga tggaatttat agagcagatg taaatcaagc ttataatact
      541 tctggaaatg ttggctatca tgtatctttt aacatgcgta acttccctaa taagaaagtc
      601 tatgtcatga tgcgggcaac gaatgatcca gagggaaaca ctaaaggcgg tgcgcaagat
      661 ttccatgaca aacgttggta tttaaatatt cctaaacgat aaaaatagct cctcgttgag
      721 gagcattaca taactatatt gacaactata aaaatcattc gataaaatag tgatgttatc
      781 gcatatcttc actatcaccc a
//
LOCUS       DASJST010000339          798 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.347, whole genome shotgun sequence.
ACCESSION   DASJST010000339 DASJST010000000
VERSION     DASJST010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.347"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            31..783
                     /locus_tag="U4B05_003061"
     CDS             31..783
                     /locus_tag="U4B05_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2052123.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT          309 a          120 c          154 g          215 t
ORIGIN      
        1 aacatataac aaaaaattag aggaaatcaa atgaatgaaa aagaatggga tgaatttgct
       61 gaagaatatt atgaaatcca gcaagaatcg caagtaacta ttgctgaaga tgtaaaaaat
      121 tatctgaaaa cggagaaagt cctgcctgct gaaacagcag cagacgttgc tggaggatct
      181 ggcagatttc tttccttagc aaaagaaatt aatcgctatg atttgatcga tttttcttca
      241 ggaatgctag aaaatgcaaa aaaagaagca gaaaaaaatg agttgccaaa tgtttccttt
      301 atcaaacagt cgttagaaga atttatccaa tcaaatgaga catatgaaat ggtttttaca
      361 gcagcaaatc cagcattggt acatcttatt caattagaaa aattgatgaa aaagtcaagc
      421 aaatggtgcg tgataatcag agtagtggaa tcaatcgatg atgtttttta tcctatcgaa
      481 aagcaactag gaatgataga agaagatcct aatacatcgc cagttgtaat ggatcaattt
      541 gaaacatact taattgatga aggattcaaa gtgaagaaaa aagattttac ttattttaca
      601 caggaatcaa ttactgaaga attgatgaag ctatactatg acgcgtacgc aaaagatcct
      661 gttgtgacca ggtacattcg caaactattc agtgaaaaac gagaaatcct tagtacgact
      721 aagctgacct atagactgct cctttggtcc gcagataaaa aaagaacctt tcacgactcg
      781 tgaagggttc tttttatg
//
LOCUS       DASJST010000340          783 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.348, whole genome shotgun sequence.
ACCESSION   DASJST010000340 DASJST010000000
VERSION     DASJST010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.348"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..716
                     /locus_tag="U4B05_003062"
     CDS             24..716
                     /locus_tag="U4B05_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN2052124.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          281 a          151 c          159 g          192 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 ctt
//
LOCUS       DASJST010000341          781 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.349, whole genome shotgun sequence.
ACCESSION   DASJST010000341 DASJST010000000
VERSION     DASJST010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.349"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..545
                     /locus_tag="U4B05_003063"
     CDS             <1..545
                     /locus_tag="U4B05_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2052125.1"
                     /translation="TIDQEKVSNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQ
                     TTETDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIAT
                     YYRKIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAK
                     KIARFNGNDYAVKVIENDLK"
BASE COUNT          266 a          105 c          152 g          258 t
ORIGIN      
        1 aaacgatcga tcaggaaaaa gtaagtaatt ctgatctttt aaatcaaatt ttattttatc
       61 aaggactgct tgatcatctt tatgcgaaaa aaccagatgc agcattattt tattttaatc
      121 aagtgttgga acaaacgacc gaaacagata tccatcactt acaagcagct gcaaacgtgg
      181 caatgatcta cttgtcaaaa ggagaattag attttgcgaa agtttatgtg gagcgtacac
      241 ttaaaatatt gagtgagact gattttgata atctgatggt ctgcattgta tattatgata
      301 tcgccaccta ttaccgtaaa atcgaggatt atgataaagc aatccaatta tgtgagaagg
      361 gtattgagta taataagaaa cataaatcga cttatgcatt ggaatacttg ctttatgaaa
      421 ttgcttcttg ccataagcaa ttaggagaag atgattattt agaaagatat atggacgcaa
      481 aaaaaatagc gcgtttcaac ggaaatgatt acgcggtaaa ggtgattgaa aacgacctga
      541 aataaagtgg ttgtgaaatc agtgttctga tttgaatacc gtttattcga aaaaaggagg
      601 tgtgactttt gtcatacctc cttttttcga tattgtttga ttcctattta ggcaacatat
      661 aacggttata aaagaagcaa tagattatga tggtatcgat tggatggata catcttggga
      721 ttcctttatc aatttgagcg tagcgtgttt ttcatttatc gataaatttg ttattcattt
      781 t
//
LOCUS       DASJST010000342          754 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.350, whole genome shotgun sequence.
ACCESSION   DASJST010000342 DASJST010000000
VERSION     DASJST010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.350"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          250 a          123 c          124 g          257 t
ORIGIN      
        1 ctgcgcgagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt gacatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcat gactgacgtg ccggacgcac
      181 gtagcgatca tcggcgttat gactatataa agtatacttt cgttgatcca gaaaggtata
      241 aagattaaag taggctacct aaaaaggtag cccggaacga attttatcac catacttgtg
      301 aaaggagata ttttttaagt tagtattaag attgtgtaat atgatgatat ctatatttta
      361 tagtatcagt gctataaaat caaaaataag tcactaatta actaccactc caattgtaag
      421 ccttttttct cacttttttt caaaaatatg gtatgctttt taatggcttc aaatataaaa
      481 gagtttaaag cgtaacacac ttatggggaa gtggtttggg gtgcgcttta aactcttctt
      541 tattattatc tcacaattta acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt
      601 tcaaatatac agaagtataa ctatgtgaaa catcctttca ttaatccata aaaggataca
      661 taaaaaagcc actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc
      721 aaaaaaattc taacacagaa cgattcaaat ggct
//
LOCUS       DASJST010000343          692 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.351, whole genome shotgun sequence.
ACCESSION   DASJST010000343 DASJST010000000
VERSION     DASJST010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.351"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            94..>692
                     /locus_tag="U4B05_003064"
     CDS             94..>692
                     /locus_tag="U4B05_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2052126.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWH"
BASE COUNT          233 a          125 c          145 g          189 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac at
//
LOCUS       DASJST010000344          691 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.352, whole genome shotgun sequence.
ACCESSION   DASJST010000344 DASJST010000000
VERSION     DASJST010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.352"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..>691
                     /locus_tag="U4B05_003065"
     CDS             10..>691
                     /locus_tag="U4B05_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HEN2052127.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
BASE COUNT          228 a          125 c          147 g          191 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata ccaatgtcgc ttaccgctgg
      301 tttttaggct actccttcga ggagaaaatc ccccatttct caacttttgg aaagaattat
      361 gttcgccgat ttagagaaac gacggtgttt gaggatattt ttgcctatat tttagaacaa
      421 gcagttaaag caggttttgt aacagaggat aatttgtatc tggattctac ccacatcaaa
      481 gctaacgcca ataaacataa gtttacgaaa gaaatgaccc atgatgaagc gaaagcctat
      541 caggatgaat tagaagatga gatcaatcgt caacgaatag aggctggaaa acgccctttt
      601 actttggact tagaaaagga agtgaagctg aaagaaagaa aaataagtaa agctgatccg
      661 gaaagcggct actacgtcaa aggcgaacga g
//
LOCUS       DASJST010000345          674 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.354, whole genome shotgun sequence.
ACCESSION   DASJST010000345 DASJST010000000
VERSION     DASJST010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.354"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..509)
                     /locus_tag="U4B05_003066"
     CDS             complement(132..509)
                     /locus_tag="U4B05_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052128.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          209 a          114 c          116 g          235 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatt
//
LOCUS       DASJST010000346          673 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.355, whole genome shotgun sequence.
ACCESSION   DASJST010000346 DASJST010000000
VERSION     DASJST010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.355"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            15..431
                     /locus_tag="U4B05_003067"
     CDS             15..431
                     /locus_tag="U4B05_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEN2052129.1"
                     /translation="MMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIP
                     ILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYIT
                     KTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          276 a           85 c           90 g          222 t
ORIGIN      
        1 tgattggaga gaaaatgatg gatcaaaaag cgtttatcga atctattgat tctacaatcg
       61 atagattaaa aaaagaaatt cgattttata atagagtaat tggtatcgga aatattataa
      121 aaatagtctt atcagctagt atcccaatat taattgatca ggcttccgaa cacaggtctt
      181 tactattgat tgtttcaatt gcttcagcaa taattacaat tatacaaagt ggtatgtctg
      241 catttaatta tcaagataaa gtacagactt cgacagagtt attaatgaaa attgaaaaag
      301 aaaagttgtt atatataacg aaaacatccc catataataa aacagatgaa gaaaatttcc
      361 atttaatcgt tacaaccctt caaactgaat taaatgatat tatctctgat tttaatcaag
      421 ttaataacta aaaatttatt taattagaaa gtagtgcaga taatactaat tgtacaatta
      481 agaaaagaga taagacttta cattcctaaa aaaatgtaaa gtcttatctc ttttcttaat
      541 gctataaatt cataataaca ttatggacaa taaatttaaa atctattcag actgtagaca
      601 aactcctaaa tttagagtgt tgtttgcagt ctgacgactc aaaaatgagt cgtataaatc
      661 aattatagta ata
//
LOCUS       DASJST010000347          652 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.356, whole genome shotgun sequence.
ACCESSION   DASJST010000347 DASJST010000000
VERSION     DASJST010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.356"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..581
                     /locus_tag="U4B05_003068"
     CDS             39..581
                     /locus_tag="U4B05_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN2052130.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
BASE COUNT          205 a          101 c          133 g          213 t
ORIGIN      
        1 ttgatattgt tgttatataa atgaaaggag ttttacaaat gattgatcca aaaatgttgg
       61 ggatgatctt cctcattaat tttgcttata tcactctgaa cacgttacgt ttcatgttga
      121 caatgaaagg ctatcgactg attgctccac ttgtcagtat ggtcgagatc acgatttata
      181 tcgtcggctt atcccttgtt ttagatcgtt tagataatcc actgaattta tttgtctatg
      241 cgttaggtta cgcagtaggt atcagtgtcg gaattttgat tgaagacaaa ttggcattag
      301 gatacatcat ggtcacaacg attttgccag caaatagcgc agaagataaa agtttgccgg
      361 gtatactgag agaacacggt tatggtgtga cacagacttc tgcaatgggg ctggaaggtg
      421 agcggctagt tctcgaaata ctttctccta gaaaaagcga acgtgaactg tatcgcctga
      481 tcaaagaaat cgaagagcgt gcttttatca tttcttatga gccaaaatat atttcaggtg
      541 gtttctggac taaaaaagtg agaaaacgac aaggaaaata actcacaaac ttaaaaactg
      601 tctaatatct aaattaaatt ttaaatgtta gacagttttt atttattatt tt
//
LOCUS       DASJST010000348          616 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.357, whole genome shotgun sequence.
ACCESSION   DASJST010000348 DASJST010000000
VERSION     DASJST010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.357"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..592)
                     /locus_tag="U4B05_003069"
                     /pseudo
     CDS             complement(1..592)
                     /locus_tag="U4B05_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877790.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          177 a          142 c           76 g          221 t
ORIGIN      
        1 aaaaaagtaa aaaaaatcgt atcccaaact ttcatctaca tttccagaaa gatcttcatg
       61 ttgataatca attcctgaat caatgaccgc taccttcaca tccttagaac ccttggtttc
      121 ttcccaagcg gatggcatgc taatctctct taaattagct tgtttactcc aattaggatc
      181 gttatcgatt tcctaaagaa tcagttgtat ttggctcctc aacatcattc aacggctcta
      241 gttcaaccac atagtttggc tctgcataat tgaccatatc agaagcatta aatgttctta
      301 ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga tctcgcttat
      361 tataatcgct ttcatttgtc ctaagagtca aatctttttc caccataact tttacaccca
      421 ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt aaactgacaa
      481 ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata atcctttctt
      541 tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac atactcaccc
      601 caacaaccaa tcctaa
//
LOCUS       DASJST010000349          615 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.358, whole genome shotgun sequence.
ACCESSION   DASJST010000349 DASJST010000000
VERSION     DASJST010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.358"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>615
                     /locus_tag="U4B05_003070"
     CDS             <1..>615
                     /locus_tag="U4B05_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2052131.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT"
BASE COUNT          260 a           53 c          106 g          196 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaa
//
LOCUS       DASJST010000350          594 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.360, whole genome shotgun sequence.
ACCESSION   DASJST010000350 DASJST010000000
VERSION     DASJST010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.360"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..441
                     /locus_tag="U4B05_003071"
     CDS             <1..441
                     /locus_tag="U4B05_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2052132.1"
                     /translation="IFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGE
                     RNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSL
                     DETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT          238 a           63 c          109 g          184 t
ORIGIN      
        1 atttttttag aaatactaaa aatgaattct gattcttact tgattttagt aggtgaaggg
       61 tcacttagaa ataaactgaa agccaaagca gtagaattgg atatagagaa aagagtaaaa
      121 tttttaggag aaagaaacga tattcccgag ttattatctg tgatggatgt ttttgtaatg
      181 ccttctcttt atgaaggtct gccaatggtt ggattagaag cacaagcagc aaaattaaaa
      241 ttagtcttag cagatactat ttccaaagat acaaagttaa caaataatgt ggattttgta
      301 tcattagatg aaacaccaca agaatgggca aaaaaagtaa taaaagagcc gtatgctaat
      361 aaaataacac aagaactaaa gagttatgat gtttcgtaca ctactgaaca aatggagagt
      421 atatatagta gagatcatta aaaatgttca agatatttta ggagtgatta tttttgaaaa
      481 tatgttattt gatattggca tacaatgata ttggcaactt aaagagattg gtcaatcgat
      541 tgaacgaaaa tgcagttttt attattcata tagataaaaa aactgatata gaac
//
LOCUS       DASJST010000351          586 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.361, whole genome shotgun sequence.
ACCESSION   DASJST010000351 DASJST010000000
VERSION     DASJST010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.361"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          259 a           83 c           97 g          147 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaa
//
LOCUS       DASJST010000352          577 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.362, whole genome shotgun sequence.
ACCESSION   DASJST010000352 DASJST010000000
VERSION     DASJST010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.362"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          222 a           75 c           73 g          207 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaataag aactaaatag gaaaacgtag acgtaatttt
      121 ctacgttttc cttttcatat atattaaata ttacggaaaa ttatataaac taatttagtg
      181 ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc tatttaactc
      241 attagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt acttaatttt
      301 gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat gatatatatc
      361 tacagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa acctataaaa
      421 tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa attatggatc
      481 acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta ttgattgcaa
      541 aattatgtga caaaaagaac tagtagaaga aggaata
//
LOCUS       DASJST010000353          563 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.363, whole genome shotgun sequence.
ACCESSION   DASJST010000353 DASJST010000000
VERSION     DASJST010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.363"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..>563
                     /locus_tag="U4B05_003072"
     CDS             200..>563
                     /locus_tag="U4B05_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052133.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIE"
BASE COUNT          191 a           64 c           87 g          221 t
ORIGIN      
        1 tttagtttac tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct tggtattgtt
       61 ttaattttta tactatctat ttttatagat gaaatggtgg aggctcatgt acttttcaat
      121 aagcgcgatg tcatttcaat tgttttctgg tattatttgg gaagcatttc ttacttgtat
      181 ttaaaaaagg agaattaata tgaagacagc tttcattgct tggactcctt tacatttgat
      241 taatatatta aatgtttatc aaaattttta ccgagaatca aaaggagatt tatttattta
      301 ttctgaattt gaaaacgcta gaagatatag tgagcgtata aaagaattag ggctatttaa
      361 tgatgttgtt tttgtgaatc accggaaaat cggttcttcc tttaaacgta aaattaactt
      421 gttaactaac cataatcaat ttttctcgga tgatttatcc gtttatgatg aaatttttat
      481 acagggggaa aattatttca caaaaatact tttctccaat gtttacaaaa aaaatccaga
      541 tgtaaaacta aattatatag aag
//
LOCUS       DASJST010000354          555 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.364, whole genome shotgun sequence.
ACCESSION   DASJST010000354 DASJST010000000
VERSION     DASJST010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.364"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>555
                     /locus_tag="U4B05_003073"
     CDS             <1..>555
                     /locus_tag="U4B05_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2052134.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCG"
BASE COUNT          204 a           87 c           97 g          167 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtgga
//
LOCUS       DASJST010000355          553 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.365, whole genome shotgun sequence.
ACCESSION   DASJST010000355 DASJST010000000
VERSION     DASJST010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.365"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            57..>553
                     /locus_tag="U4B05_003074"
     CDS             57..>553
                     /locus_tag="U4B05_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEN2052135.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          202 a          103 c           90 g          158 t
ORIGIN      
        1 tcccttctgt agaataaagt taacgacaac caattcacag aaaagaggac ttccctatga
       61 atgattttac tacagaaatt gtgcaaactc tagtcactaa aggcgattta aatgaattat
      121 tccgttcgca cttagaaaaa gcgataaaca cactcctacg gactgaatta acggcttttt
      181 tagattacga aaaatatgat cgcactggtt ttaattcagg taattcgaga aacggttctt
      241 actttcgatc aatcaaaacc gaatatggtg aattaacatt ggaaatacct agagatcgta
      301 atggtgagtt taaacaacaa actttaccag cctacaaaag aacaaacgat acattggaaa
      361 ccactattat ccatttattc gaaaaaggtg ttacgatgtc tgaaattgct gatttgatcg
      421 aaaaaatgta cggtcatcac tatactccac aaaccatgtc caacatgact aaagttctga
      481 ctgaagaagt aaatgccttt aaatccagag ccttaaatga taagtatgtc gctattttta
      541 tggacgctac tta
//
LOCUS       DASJST010000356          548 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.366, whole genome shotgun sequence.
ACCESSION   DASJST010000356 DASJST010000000
VERSION     DASJST010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.366"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(91..>548)
                     /locus_tag="U4B05_003075"
     CDS             complement(91..>548)
                     /locus_tag="U4B05_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2052136.1"
                     /translation="LDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRG
                     KEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRL
                     ELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          154 a          129 c          101 g          164 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagta
//
LOCUS       DASJST010000357          534 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.367, whole genome shotgun sequence.
ACCESSION   DASJST010000357 DASJST010000000
VERSION     DASJST010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.367"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>534
                     /locus_tag="U4B05_003076"
     CDS             <1..>534
                     /locus_tag="U4B05_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2052137.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DASJST010000358          514 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.368, whole genome shotgun sequence.
ACCESSION   DASJST010000358 DASJST010000000
VERSION     DASJST010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.368"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..>514)
                     /locus_tag="U4B05_003077"
     CDS             complement(107..>514)
                     /locus_tag="U4B05_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN2052138.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DASJST010000359          509 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.369, whole genome shotgun sequence.
ACCESSION   DASJST010000359 DASJST010000000
VERSION     DASJST010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.369"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="U4B05_003078"
     CDS             complement(<1..132)
                     /locus_tag="U4B05_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2052139.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DASJST010000360          500 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.370, whole genome shotgun sequence.
ACCESSION   DASJST010000360 DASJST010000000
VERSION     DASJST010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.370"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(262..411)
                     /locus_tag="U4B05_003079"
     CDS             complement(262..411)
                     /locus_tag="U4B05_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052140.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          160 a           77 c           81 g          182 t
ORIGIN      
        1 ttaatttgac aattaagatt atattaaata ttatacagat aataacggaa tattatcatc
       61 gaaacagcta acaaaattgt cataaaaaac gagatggttg atattagaac ttgtgttttt
      121 gtttaggtct agagataaag agagacaatt tccaaatata tgtaatgaat acaaaaagac
      181 gtttgtcgcg gattaccgtt acaaacgtct tttattgatt ttccgagatc tcatcagtcg
      241 ccatttggaa tggttcactc cttatctaga ttcgtttaat gctcggactg cttgttcgat
      301 tgtaatacca aacgcaacgt tattttcgtt ttgtgcatca atcgccataa aacggttaga
      361 agttgtatca aaagctacgc ggtattgcat atttgttttt gtaaacgtca taaaattcat
      421 ctcctttgta atgtaattta gttcttgaaa gtttccttta aactttcgaa cttataccaa
      481 gtttagcatc gtacgttttt
//
LOCUS       DASJST010000361          493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.371, whole genome shotgun sequence.
ACCESSION   DASJST010000361 DASJST010000000
VERSION     DASJST010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.371"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>493
                     /locus_tag="U4B05_003080"
     CDS             <1..>493
                     /locus_tag="U4B05_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2052141.1"
                     /translation="KNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIK
                     DNEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIF
                     RAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIG
                     MFAP"
BASE COUNT          196 a           59 c           75 g          163 t
ORIGIN      
        1 ataaaaaccg tgttaaaatt tattggggtg gtttttccat tgtacaagca gaaatcaatt
       61 tagtaaagag agcattacaa aatgagaaat atttaaaata tgtattatta tctggtgctg
      121 attatccaat caaagataat gagtatattt ataattattt taaaaagaat agttcagtgg
      181 agtttattcg tggaatagat ctagatcaaa ttaaacataa agaattttat tataaacata
      241 tagatgtata tcaaaaacat gattatccta gaattaatag gaataacaca actgcattta
      301 aaatttttag agctataatt aatcgttgtc tgaggatgat taaacttccc ccaaaaatta
      361 gacaccataa atttgatttg tatcatggtt ctcaatggtg ggctttgtcg aaagaatgcc
      421 taacagaatt aatacaaatg tatgaacaaa atcaacaaga ctacctgaat tttaaaatag
      481 gtatgtttgc ccc
//
LOCUS       DASJST010000362          493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.372, whole genome shotgun sequence.
ACCESSION   DASJST010000362 DASJST010000000
VERSION     DASJST010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.372"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..>493)
                     /locus_tag="U4B05_003081"
     CDS             complement(54..>493)
                     /locus_tag="U4B05_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2052142.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          133 a           92 c          115 g          153 t
ORIGIN      
        1 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
       61 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
      121 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
      181 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
      241 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
      301 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
      361 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
      421 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
      481 tcggcttttt tga
//
LOCUS       DASJST010000363          487 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.373, whole genome shotgun sequence.
ACCESSION   DASJST010000363 DASJST010000000
VERSION     DASJST010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.373"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>487)
                     /locus_tag="U4B05_003082"
     CDS             complement(<1..>487)
                     /locus_tag="U4B05_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEN2052143.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DASJST010000364          481 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.374, whole genome shotgun sequence.
ACCESSION   DASJST010000364 DASJST010000000
VERSION     DASJST010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.374"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..97)
                     /locus_tag="U4B05_003083"
     tRNA            complement(24..97)
                     /locus_tag="U4B05_003083"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Ile,seq:cat)
BASE COUNT          163 a           77 c           95 g          146 t
ORIGIN      
        1 aacgattcaa ttggctacat taatgtaccc tgtaggactc gaacctacga ccggacggtt
       61 atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac ataaagccat
      121 aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct tggagtgtga
      181 ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga gacagataaa
      241 catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta
      301 gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa
      361 atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt
      421 acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag
      481 c
//
LOCUS       DASJST010000365          480 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.375, whole genome shotgun sequence.
ACCESSION   DASJST010000365 DASJST010000000
VERSION     DASJST010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.375"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            91..456
                     /locus_tag="U4B05_003084"
     CDS             91..456
                     /locus_tag="U4B05_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052144.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          143 a           87 c           73 g          177 t
ORIGIN      
        1 tagaaataat gaaaaagtaa ttgattattg caggattcaa tttacaataa aactaacaaa
       61 ataataacac ttgttaggtg gtgatgaaag atgataaaaa cttgctggaa aaaccttccg
      121 ttgcttttat cttttgttcc ttatgtgcat ttcgccttac tgcttgattt tcgttatcat
      181 tccgtaagcg ggttcatcac attgatattt ttatctcttt ttgctggata ttattttcaa
      241 aggaacaggc gtattatatc tttattcatt gccaatatta tatctactgt cacttcctat
      301 ttattttgcg ccaattttac tgaatggcgt tacttctatc atcctttaaa accgacccag
      361 ctgatcttgc ttcttgcagg tatttactta gttccacaaa tccttgggtc gttatgggcg
      421 gtagccttat cttataaaaa agctcggcat ccataaaaac aaaccatata tgtacaaaga
//
LOCUS       DASJST010000366          476 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.376, whole genome shotgun sequence.
ACCESSION   DASJST010000366 DASJST010000000
VERSION     DASJST010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.376"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>476
                     /locus_tag="U4B05_003085"
     CDS             22..>476
                     /locus_tag="U4B05_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2052145.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DASJST010000367          475 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.377, whole genome shotgun sequence.
ACCESSION   DASJST010000367 DASJST010000000
VERSION     DASJST010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.377"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..258
                     /locus_tag="U4B05_003086"
     CDS             <1..258
                     /locus_tag="U4B05_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052146.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="U4B05_003087"
     CDS             260..397
                     /locus_tag="U4B05_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2052147.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT          184 a           61 c          105 g          125 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaagaagc
      361 agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt
      421 caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga tcaat
//
LOCUS       DASJST010000368          471 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.378, whole genome shotgun sequence.
ACCESSION   DASJST010000368 DASJST010000000
VERSION     DASJST010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.378"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..471
                     /locus_tag="U4B05_003088"
                     /pseudo
     CDS             1..471
                     /locus_tag="U4B05_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          175 a           96 c           97 g          103 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaaaat tctaaatatt
      241 tctcgtcaaa cctactatta tcaagcgaaa ccgatcgaaa atgagtccga cttagaagag
      301 atcgttcagg aagagtttat tcgaaaccga aaggcttacg gtacccgaaa attgaagaag
      361 tgtttagcaa agcgtgggct tcaactcagt cggcgccgaa tcggtcgaat catgaaacgc
      421 cgcggattga catctaccta tacgatcgct cattttaaag ggcaacgaac a
//
LOCUS       DASJST010000369          462 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.379, whole genome shotgun sequence.
ACCESSION   DASJST010000369 DASJST010000000
VERSION     DASJST010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.379"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..430)
                     /locus_tag="U4B05_003089"
     CDS             complement(<1..430)
                     /locus_tag="U4B05_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2052148.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DASJST010000370          462 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.380, whole genome shotgun sequence.
ACCESSION   DASJST010000370 DASJST010000000
VERSION     DASJST010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.380"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..422)
                     /locus_tag="U4B05_003090"
     CDS             complement(<1..422)
                     /locus_tag="U4B05_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2052149.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRI"
BASE COUNT          134 a           75 c           71 g          182 t
ORIGIN      
        1 ttaattctta ctaatgactt caatttagga actttgatga atttactatc aatcaaagca
       61 actgtaccat tttgattatt cgttgtataa ctctgaacag gatttttctt acttttgaaa
      121 cgtggaaatc caacggattt atcccgaaaa aaattcttgt atgctttatc taaattaagt
      181 tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa acggaaattc tttcttgtat
      241 ttagctggtg tcggaaaagt cattttttta gaagaatcat ttttaacttc ttcatacgcc
      301 ttttttcggt catcaagcat tagattgtag accttacgga cacaaccaaa agatttggca
      361 aagaagattt cttgttcctc tgttggatag attctgaatt tgtatgcttt tagttgttcc
      421 ataaagttca cctactttct atttatgatt gtttccttga tt
//
LOCUS       DASJST010000371          462 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.381, whole genome shotgun sequence.
ACCESSION   DASJST010000371 DASJST010000000
VERSION     DASJST010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.381"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(18..>462)
                     /locus_tag="U4B05_003091"
     CDS             complement(18..>462)
                     /locus_tag="U4B05_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2052150.1"
                     /translation="TFYAPCSGKIISITEVNDPVFSQKLLGDGFAVIPDGSKIYAPLA
                     GRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQENDEVTPETSLVDV
                     DFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT          159 a           81 c           65 g          157 t
ORIGIN      
        1 tttttatata cactttttta aaaagtgatt tttccacatg aattcccatg agcaattcga
       61 ccattatcta ttattaaatc attgaactga tttttctccg gaaaaacaac tataacagaa
      121 gtatctttac cagattttat tattttgtca aaatccacat caaccaacga ggtttcagga
      181 gtaacctcat cattttcttg aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta
      241 tcaattccaa tatgaatcaa ataatcaatg cctgagttgc tttttatatt aatagcatgt
      301 ttagtaggaa atatatttgt tactcgacca gccaatggag cataaatttt tgaaccatca
      361 ggtatgacgg caaaaccatc acctagtaat ttttgtgaaa agacaggatc atttacttct
      421 gtgatggaaa tgatttttcc ggaacaaggt gcataaaaag tt
//
LOCUS       DASJST010000372          461 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.382, whole genome shotgun sequence.
ACCESSION   DASJST010000372 DASJST010000000
VERSION     DASJST010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.382"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          127 a          116 c           65 g          153 t
ORIGIN      
        1 actaaacccc aagttaaacc cctccttcgc gctgtcgcta ataaaaagag tgtaaacctt
       61 tctgttactc tttgacaaag agtaacattt tcctaattgt gacatggctg tcacaattcc
      121 aaatgctctc cgagggtatt cgctgtcaca agtgacaacg aaaaaaggag ccgaaagcgt
      181 gtcacgctct caactccttt tacgctttat tttgcttcac gcaaaataat cgcttatcgt
      241 aaactcacgt cattaaacat tgacgtaaat ttactctcac tcatctgact tttcttattc
      301 ggtatcactt ctctgtcacg tccaaaccca aaagccacgt gtctaattct attttcaagt
      361 cgtttgcaga ttccttaaac gtctctcgta aatgattcgt caactcatcg ttttggcttt
      421 cttctttcaa caatttgcgc aatctatatt tcaaatttct g
//
LOCUS       DASJST010000373          460 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.383, whole genome shotgun sequence.
ACCESSION   DASJST010000373 DASJST010000000
VERSION     DASJST010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.383"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..91)
                     /locus_tag="U4B05_003092"
     tRNA            complement(20..91)
                     /locus_tag="U4B05_003092"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="U4B05_003093"
     tRNA            complement(113..200)
                     /locus_tag="U4B05_003093"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="U4B05_003094"
     tRNA            complement(225..298)
                     /locus_tag="U4B05_003094"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="U4B05_003095"
     tRNA            complement(312..382)
                     /locus_tag="U4B05_003095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="U4B05_003096"
     tRNA            complement(384..459)
                     /locus_tag="U4B05_003096"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DASJST010000374          451 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.384, whole genome shotgun sequence.
ACCESSION   DASJST010000374 DASJST010000000
VERSION     DASJST010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.384"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          146 a           87 c           51 g          167 t
ORIGIN      
        1 aaaaaataaa aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga
       61 tctcattctt tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt
      121 atatattcgt attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg
      181 tccgttactt aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt
      241 ttcattaatt gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc
      301 aaactcattc attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac
      361 atcactttat tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat
      421 acactactat tcagcccatt caacgaccaa t
//
LOCUS       DASJST010000375          443 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.385, whole genome shotgun sequence.
ACCESSION   DASJST010000375 DASJST010000000
VERSION     DASJST010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.385"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..369
                     /locus_tag="U4B05_003097"
                     /pseudo
     CDS             1..369
                     /locus_tag="U4B05_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT          207 a           79 c           70 g           87 t
ORIGIN      
        1 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
       61 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
      121 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
      181 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
      241 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
      301 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
      361 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
      421 aaaatgaccc caaaatacca gat
//
LOCUS       DASJST010000376          427 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.386, whole genome shotgun sequence.
ACCESSION   DASJST010000376 DASJST010000000
VERSION     DASJST010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.386"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>427)
                     /locus_tag="U4B05_003098"
     CDS             complement(<1..>427)
                     /locus_tag="U4B05_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2052151.1"
                     /translation="STLSLISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRG
                     GGNVKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTV
                     MLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKD"
BASE COUNT          114 a           76 c           50 g          187 t
ORIGIN      
        1 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
       61 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
      121 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
      181 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
      241 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
      301 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
      361 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
      421 agttgac
//
LOCUS       DASJST010000377          424 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.387, whole genome shotgun sequence.
ACCESSION   DASJST010000377 DASJST010000000
VERSION     DASJST010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.387"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..316
                     /locus_tag="U4B05_003099"
     CDS             2..316
                     /locus_tag="U4B05_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052152.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          138 a           53 c           64 g          169 t
ORIGIN      
        1 tttggatttt tacagtaatt ttatcttaat aatagcaata ttattacttc taaatatttg
       61 gttttttgat aagagtagaa atgcagggat aggttttaga accaaacgaa gtacgtcttc
      121 tgaaaaaaag tgggtatatt cacagactat tttttacgga ggggttattt caatcagttt
      181 actttcttca acactttatt cttttaatgt tatagacgtt tccatgtcaa attttatatc
      241 tattattgga atccttattt ctgcaattat tacacaacta cttcttgtgt ttgaagagaa
      301 gtcaaaaaat aattaaagca attatgcctt ttagtacagg aattattact tcttacaaat
      361 gtatagcgaa tgtgaagcct gctgttttaa ttgttctaga aaaacttttg agtgaacaat
      421 tatt
//
LOCUS       DASJST010000378          423 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.388, whole genome shotgun sequence.
ACCESSION   DASJST010000378 DASJST010000000
VERSION     DASJST010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.388"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          151 a           53 c           74 g          145 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaaaaga ggactacctg
      421 tat
//
LOCUS       DASJST010000379          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.389, whole genome shotgun sequence.
ACCESSION   DASJST010000379 DASJST010000000
VERSION     DASJST010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.389"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..390
                     /locus_tag="U4B05_003100"
     CDS             <1..390
                     /locus_tag="U4B05_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2052153.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          115 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatt
//
LOCUS       DASJST010000380          385 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.390, whole genome shotgun sequence.
ACCESSION   DASJST010000380 DASJST010000000
VERSION     DASJST010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.390"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..>385
                     /locus_tag="U4B05_003101"
     CDS             12..>385
                     /locus_tag="U4B05_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052154.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGS"
BASE COUNT          130 a           52 c           95 g          108 t
ORIGIN      
        1 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa
       61 aaacgcatgt agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag
      121 ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga
      181 cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga
      241 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
      301 aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat cggacaccaa
      361 ttagttttga acaactggtt gggtc
//
LOCUS       DASJST010000381          384 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.391, whole genome shotgun sequence.
ACCESSION   DASJST010000381 DASJST010000000
VERSION     DASJST010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.391"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(168..>384)
                     /locus_tag="U4B05_003102"
     CDS             complement(168..>384)
                     /locus_tag="U4B05_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052155.1"
                     /translation="AQEQKLVPVTMVQINKANQFIFLFEHGKAPLAMKDIFLFLMKNR
                     EKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          118 a           66 c           58 g          142 t
ORIGIN      
        1 cttttatttt aataaaaaga aacagccaat aaagtagaaa tctcgtgggg gagaacacct
       61 ttattggcct gttcaacgaa tattatactg tatcatgaca cttttttgcc aaaaatgtaa
      121 aaaatggttg ttttgacatc ataaacgtct tgttttctta tttttactta tatcaccact
      181 ttattttcta tctctttcac tccataaaga ggaatgagtt cttcttttga ctgataataa
      241 acagctttct cacggttctt catcaaaaaa agaaagatgt ctttcatagc aagtggtgct
      301 ttgccgtgtt caaataaaaa gataaattga tttgctttat ttatctggac cattgtcaca
      361 ggcaccagtt tttgttcttg agcc
//
LOCUS       DASJST010000382          373 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.392, whole genome shotgun sequence.
ACCESSION   DASJST010000382 DASJST010000000
VERSION     DASJST010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.392"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>373
                     /locus_tag="U4B05_003103"
     CDS             <1..>373
                     /locus_tag="U4B05_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2052156.1"
                     /translation="QIVDKHDENIAYIEILKYQEGQGRIDFNPNKINQFLASSMKNFI
                     HDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPVSFKPIYGRSGKLESAYWGSRASE
                     RQIRLYNKKLEQETKRKVVPKE"
BASE COUNT          126 a           60 c           77 g          110 t
ORIGIN      
        1 gcaaattgtg gataagcatg atgagaatat tgcttacatt gaaattttga aatatcaaga
       61 aggtcaaggg cgaattgact ttaatccgaa taaaattaat cagtttttag ctagctctat
      121 gaagaatttt atccatgatt tatttttgga gccgcatttt tcacgtgcag atattgcttg
      181 cgatataatt gacgtaccag atgatttcat tacccaatat cgggtggttg atcctgtatc
      241 gttcaaaccg atttacgggc gttctggaaa actagaatca gcttattggg gtagccgtgc
      301 cagcgaacga caaattcggc tatacaataa aaaattggaa caagaaacca aacgaaaagt
      361 tgtaccaaag gaa
//
LOCUS       DASJST010000383          371 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.393, whole genome shotgun sequence.
ACCESSION   DASJST010000383 DASJST010000000
VERSION     DASJST010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.393"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>371
                     /locus_tag="U4B05_003104"
     CDS             <1..>371
                     /locus_tag="U4B05_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2052157.1"
                     /translation="KNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQL
                     TFPELEQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQK
                     ADQIIHYAQCAYPAVEKDSIH"
BASE COUNT          126 a           84 c           64 g           97 t
ORIGIN      
        1 aaaaatcaac gtcagccaaa agaagtacaa agcaattttt acttagagat gcgcgattta
       61 gcccgttttt atcaagaaat tgagaagaaa atcactcgtc tgcgcatgga tttacacaac
      121 tgccttcagt tgacttttcc agagcttgaa cagttctttt taaacaggct gaccccttac
      181 gcattgatat tgatccgtct ttttccccat ccagattttg tattggcatc tactcggaca
      241 aaaataaaaa ataaactgat caacgaaaca cggaaaaaaa tctccgcgaa tcgtgccgag
      301 caaaaggcag atcaaatcat ccactatgca cagtgtgcct atccagctgt tgaaaaagac
      361 agtatccact g
//
LOCUS       DASJST010000384          344 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.394, whole genome shotgun sequence.
ACCESSION   DASJST010000384 DASJST010000000
VERSION     DASJST010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.394"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..190)
                     /locus_tag="U4B05_003105"
     CDS             complement(29..190)
                     /locus_tag="U4B05_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN2052158.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            260..>344
                     /locus_tag="U4B05_003106"
     CDS             260..>344
                     /locus_tag="U4B05_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2052159.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
BASE COUNT          112 a           80 c           42 g          110 t
ORIGIN      
        1 tttttatcac tttcctaact actcctttct attttcttac tccataacta attgcatcca
       61 ctggacaagc atttttacat cgtccacatc tgatacactc cagatgattg caattctcaa
      121 ctgaatcttt attcatttgg caaacttttt tacattttcc acaattaata cacttatcct
      181 tatcctgaat cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa
      241 acacactgga ggatttttca tgccaagacg tcaacgaaga acctattcaa aagaattcaa
      301 acaacaaatc gtcgatctct atctcgctgg taagccttgc gcag
//
LOCUS       DASJST010000385          333 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.395, whole genome shotgun sequence.
ACCESSION   DASJST010000385 DASJST010000000
VERSION     DASJST010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.395"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          124 a           54 c           59 g           96 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaa
//
LOCUS       DASJST010000386          323 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.396, whole genome shotgun sequence.
ACCESSION   DASJST010000386 DASJST010000000
VERSION     DASJST010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.396"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U4B05_003107"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U4B05_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN2052160.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DASJST010000387          321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.397, whole genome shotgun sequence.
ACCESSION   DASJST010000387 DASJST010000000
VERSION     DASJST010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.397"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..321)
                     /locus_tag="U4B05_003108"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="U4B05_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DASJST010000388          311 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.398, whole genome shotgun sequence.
ACCESSION   DASJST010000388 DASJST010000000
VERSION     DASJST010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.398"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            141..>311
                     /locus_tag="U4B05_003109"
     CDS             141..>311
                     /locus_tag="U4B05_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052161.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKK"
BASE COUNT          138 a           42 c           43 g           88 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa a
//
LOCUS       DASJST010000389          309 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.399, whole genome shotgun sequence.
ACCESSION   DASJST010000389 DASJST010000000
VERSION     DASJST010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.399"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          135 a           42 c           25 g          107 t
ORIGIN      
        1 aaaaataaat taagaactac cttatgcata taaaacatac taccattact agtaatatat
       61 actaccttat ttcagtagta tatattatat tcttcttata agacaaggct ttaggctaga
      121 aaacaataga ttatatacta ctaaaaaata caataagtac taccaatgtc agatatatat
      181 aataattatg tactaccatt aaaattaact tatttaataa gatactactg tgttaatctg
      241 aaaaaaatta ctttaaagta gttacatact acctgttaaa ttagatacta ccattaatac
      301 ttaaattag
//
LOCUS       DASJST010000390          303 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.400, whole genome shotgun sequence.
ACCESSION   DASJST010000390 DASJST010000000
VERSION     DASJST010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.400"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>303
                     /locus_tag="U4B05_003110"
     CDS             <1..>303
                     /locus_tag="U4B05_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2052162.1"
                     /translation="DFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKK
                     LEQERKKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYLPGDVKPV"
BASE COUNT           98 a           55 c           76 g           74 t
ORIGIN      
        1 atgattttat tacccagtat cgaatcgttg accctgtgtc ttttaagcca atttatggcc
       61 gttctggcaa attagaaaca gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga
      121 tgtacaacaa aaagcttgaa caggaacgaa aaaaacagat tgtaccaaaa gaaatcgaaa
      181 catggtggcg tatagaaatg cagctaagac gtggcaaagc aacagattgg catgcgatgg
      241 ttcgggaaag tttagatagc tttgcaagtc ctcattactt gcctggagat gtaaaacctg
      301 ttg
//
LOCUS       DASJST010000391          301 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.401, whole genome shotgun sequence.
ACCESSION   DASJST010000391 DASJST010000000
VERSION     DASJST010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.401"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>301
                     /locus_tag="U4B05_003111"
     CDS             <1..>301
                     /locus_tag="U4B05_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN2052163.1"
                     /translation="SVKDLRKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKE
                     IPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          113 a           36 c           66 g           86 t
ORIGIN      
        1 aatcggttaa agatttacgt aaactaccaa caaaggacat aaatggcaag gattttacaa
       61 gtaaagattt tgagaagtat gatcttacta tggtaaatgt atttgcaact tggtgtacag
      121 cttgtgtaaa ggaaattcct gatttggttg aggttcagaa tgaaatgaaa agcaagggtg
      181 taaatatcgt tggagttgtt acagatcctg ttgatgataa cggtgaaaac aaggaagcaa
      241 ttgaaaagtc aaagttaata catgaaaaga caaaggcttc atatcctttc ttaatgccag
      301 a
//
LOCUS       DASJST010000392          301 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.402, whole genome shotgun sequence.
ACCESSION   DASJST010000392 DASJST010000000
VERSION     DASJST010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.402"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>301
                     /locus_tag="U4B05_003112"
     CDS             <1..>301
                     /locus_tag="U4B05_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2052164.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKD"
BASE COUNT          102 a           63 c           55 g           81 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaagat
      301 g
//
LOCUS       DASJST010000393          299 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.403, whole genome shotgun sequence.
ACCESSION   DASJST010000393 DASJST010000000
VERSION     DASJST010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.403"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          106 a           33 c           46 g          114 t
ORIGIN      
        1 gactacctgt attagtcctc ttttttgacc agattttgac cagtctattt tacattctaa
       61 tatattcata tgagctaatt ttatgattaa atggtaagtt tttccttgtt tgttttagtt
      121 taaactagtg tgtaattcaa aaatctatat ggaaggatct gtggggtaaa tcattataca
      181 aaagtaaaat tttacttatt aacaggctgt agaaatataa aaaagaacta aaatttaact
      241 aaattatgtg ctattcttaa ggaaagccaa gtgtatgaga aagtttctga gaatataat
//
LOCUS       DASJST010000394          298 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.404, whole genome shotgun sequence.
ACCESSION   DASJST010000394 DASJST010000000
VERSION     DASJST010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.404"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..271
                     /locus_tag="U4B05_003113"
                     /pseudo
     CDS             161..271
                     /locus_tag="U4B05_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          103 a           47 c           38 g          110 t
ORIGIN      
        1 catctttcag cattttacta tatttggtat atagtgctaa ggttcatatt cgattgacaa
       61 gagaatataa attgatctat ttctatttcc taagttgagt aaacataact ttgcccttta
      121 taaatatctt ccctctctct cttctctcta tattttatat ttgaataatc atatcaaggt
      181 attccaacga aatgccaatg gatttcgaag cttttataac tttaaattac ggatcatgat
      241 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttct
//
LOCUS       DASJST010000395          294 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.405, whole genome shotgun sequence.
ACCESSION   DASJST010000395 DASJST010000000
VERSION     DASJST010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.405"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           90 a           53 c           72 g           79 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actccgagtc
       61 acaaggagca atttcaaaaa acagcttctc aggtttgttg actattgtga cttgtgaccg
      121 aggagttggc aggagaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga
      181 gtattagaga tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata
      241 ccgaaggaca gctgattgaa caccgtttat tcggaaaaag ggactgtgac acga
//
LOCUS       DASJST010000396          293 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.406, whole genome shotgun sequence.
ACCESSION   DASJST010000396 DASJST010000000
VERSION     DASJST010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.406"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..>293)
                     /locus_tag="U4B05_003114"
     CDS             complement(164..>293)
                     /locus_tag="U4B05_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2052165.1"
                     /translation="NQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          101 a           42 c           36 g          114 t
ORIGIN      
        1 aacatccttt ctatcactat tagattcata tctctttttt tgagttaaac cagaaataac
       61 tttttcaaaa taaaatttta acgccaatcc atgagtcaat tcaaaaaaag aaaaaagatt
      121 ctttgttttc tcagaagttc aattctttat tatttatgat ttgtcaaatt aagctagaat
      181 aaaatgcttc ttgtacatag ctcaaaaata tttcgattgg tgttctgtaa ttcaatgatt
      241 ttcttggaat atgattacgt tgattgctga cactggaaat aaatgtctga ttc
//
LOCUS       DASJST010000397          290 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.407, whole genome shotgun sequence.
ACCESSION   DASJST010000397 DASJST010000000
VERSION     DASJST010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.407"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          128 a           38 c           23 g          101 t
ORIGIN      
        1 taaaaaaagc ctttaaaatt aaaacaattg gcactgctcc taattttaaa taacaaaaaa
       61 attactagat aatctaaaaa caggatggca ttatgagaac aataactaaa cttcctatat
      121 cacatatttt gtaattagtg aaaagcaaat tgactactat cataagttaa tgactctcat
      181 cttaatattt aaaatatatt catatttttc tattttatct aaatgcgatt ttcatatcta
      241 ataatagaaa aataaacaat taacaataaa aaagtcttca tttattgtta
//
LOCUS       DASJST010000398          283 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.408, whole genome shotgun sequence.
ACCESSION   DASJST010000398 DASJST010000000
VERSION     DASJST010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.408"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          115 a           52 c           26 g           90 t
ORIGIN      
        1 ctgttaatga ttatagttaa gataaaacta atcattgctc caatcagtag ccaagaaaaa
       61 cgcattttaa aaaaattttt ctttataaaa acctcataca aaactataat tccaccccaa
      121 gcaaaagcta ttttcatcgg gatattagtt acattaaaag tactaggcat aaaataaagc
      181 agacaaatga ttaaatttat tactcgaaac gcatctcgac ttaataaact cttctctaaa
      241 acaggtacta ttctattcat ctatatttcc aactttcaaa att
//
LOCUS       DASJST010000399          276 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.409, whole genome shotgun sequence.
ACCESSION   DASJST010000399 DASJST010000000
VERSION     DASJST010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.409"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          108 a           44 c           42 g           82 t
ORIGIN      
        1 taggagtgag catttttcga aaaatactaa aaatttttca aaataaaaaa aggcatacag
       61 acaataaact gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct
      121 gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact
      181 ttgcttggta agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat
      241 tccaataaat aggaaggatt acaaaatttg aaacca
//
LOCUS       DASJST010000400          269 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.410, whole genome shotgun sequence.
ACCESSION   DASJST010000400 DASJST010000000
VERSION     DASJST010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.410"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           95 a           32 c           37 g          105 t
ORIGIN      
        1 agaaaaaagg cttataatta ggatttagag taattaatta gatgtttatt ttataggact
       61 gatactatag attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg
      121 aataagataa taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct
      181 ttctggatca acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt
      241 aacgataatg ctgctaaaat atcttttta
//
LOCUS       DASJST010000401          266 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.411, whole genome shotgun sequence.
ACCESSION   DASJST010000401 DASJST010000000
VERSION     DASJST010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.411"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..>266)
                     /locus_tag="U4B05_003115"
     CDS             complement(34..>266)
                     /locus_tag="U4B05_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2052166.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DASJST010000402          266 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.412, whole genome shotgun sequence.
ACCESSION   DASJST010000402 DASJST010000000
VERSION     DASJST010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.412"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>266
                     /locus_tag="U4B05_003116"
     CDS             <1..>266
                     /locus_tag="U4B05_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2052167.1"
                     /translation="NEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAA
                     KVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNL"
BASE COUNT          108 a           29 c           58 g           71 t
ORIGIN      
        1 ataatgagaa aggtgcagat gaaacagtag aagtagaaat taaaggtcta ttcgatgggc
       61 ataatagcgg tggagtaagt gcagcacaag aactatacga aaatacatta atcactgata
      121 ttcatacagc tgctaaagtt tatggtaata ccgaagatac agctgtatac caagatgcaa
      181 cattctttgt aaaaggtgat aagaatcttg atagtgtaat aaaagatctt gggaaattag
      241 atataaattg gagagaatat aatttg
//
LOCUS       DASJST010000403          264 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.413, whole genome shotgun sequence.
ACCESSION   DASJST010000403 DASJST010000000
VERSION     DASJST010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.413"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            113..>264
                     /locus_tag="U4B05_003117"
     CDS             113..>264
                     /locus_tag="U4B05_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052168.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWV"
BASE COUNT           98 a           43 c           49 g           74 t
ORIGIN      
        1 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
       61 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      121 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      181 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      241 ttttgataaa aaacagtggg ttga
//
LOCUS       DASJST010000404          261 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.414, whole genome shotgun sequence.
ACCESSION   DASJST010000404 DASJST010000000
VERSION     DASJST010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.414"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>261
                     /locus_tag="U4B05_003118"
     CDS             <1..>261
                     /locus_tag="U4B05_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052169.1"
                     /translation="PGQAATITIGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPG
                     INATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNATTTT"
BASE COUNT           87 a           54 c           56 g           64 t
ORIGIN      
        1 tcctggccaa gcagcaacaa ttaccatagg aaccgtttct agtggttcaa ccgcttctgt
       61 tacgaatgta ggaacatcat ctgcagctag atttaatttt gttttaccaa agggagataa
      121 aggtgatcct ggaataaatg ccacaactac agctgtagct acaactacag caaatgggtt
      181 gatgtcgtcc acagataaaa ctaagttgga tggaattgca gctggagcac agaaaaatcc
      241 aggtaatgct acaactacga c
//
LOCUS       DASJST010000405          258 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.415, whole genome shotgun sequence.
ACCESSION   DASJST010000405 DASJST010000000
VERSION     DASJST010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.415"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..>258
                     /locus_tag="U4B05_003119"
     CDS             98..>258
                     /locus_tag="U4B05_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2052170.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
BASE COUNT          100 a           48 c           39 g           71 t
ORIGIN      
        1 attacgccga ttgtaaaatt aagctagaca actaaaaaag tcatttgtgc tacactcaaa
       61 atagttccga ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac
      121 ggatgaactt gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc
      181 ccattgcttg aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg
      241 aaaatcagcc ttagaata
//
LOCUS       DASJST010000406          257 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.416, whole genome shotgun sequence.
ACCESSION   DASJST010000406 DASJST010000000
VERSION     DASJST010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.416"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..155
                     /locus_tag="U4B05_003120"
     CDS             39..155
                     /locus_tag="U4B05_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEN2052171.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          112 a           30 c           33 g           82 t
ORIGIN      
        1 aaagtagata gaaataaaaa aacaaagact aaacatatat aattggatta aacgcaatat
       61 ctgcaaaata ttgtaaattt aaagcctttg tagaaaaatt tactgaaaga caaactgata
      121 agattattca gtatcatatt acctatactg attaaaattt tttgaaacgg gatactaaat
      181 aaaaaattta tttataacag aactaattat ttaatgcgga tgcgccaata cttggtcctg
      241 caagagctat ttatttt
//
LOCUS       DASJST010000407          257 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.417, whole genome shotgun sequence.
ACCESSION   DASJST010000407 DASJST010000000
VERSION     DASJST010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.417"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..101
                     /locus_tag="U4B05_003121"
     CDS             <1..101
                     /locus_tag="U4B05_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEN2052172.1"
                     /translation="NKEKCWVNWGQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          103 a           30 c           49 g           75 t
ORIGIN      
        1 gtaacaaaga aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt
       61 ggggagataa tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga
      121 tcacacaaaa aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac
      181 gaaagcatta ttagatatta ctgatgtttt atttcaggta tataaaaaaa ttgatacagc
      241 aaaatatcct gaaattc
//
LOCUS       DASJST010000408          253 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.418, whole genome shotgun sequence.
ACCESSION   DASJST010000408 DASJST010000000
VERSION     DASJST010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.418"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..>253
                     /locus_tag="U4B05_003122"
     CDS             28..>253
                     /locus_tag="U4B05_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2052173.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DASJST010000409          252 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.419, whole genome shotgun sequence.
ACCESSION   DASJST010000409 DASJST010000000
VERSION     DASJST010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.419"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..193)
                     /locus_tag="U4B05_003123"
     CDS             complement(<1..193)
                     /locus_tag="U4B05_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN2052174.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTG"
BASE COUNT           94 a           36 c           26 g           96 t
ORIGIN      
        1 caccagtaaa gaaatggaaa tctttagttt ttttaatatt atcagttagt aactttttat
       61 aatcataaaa aaaataagta gttctatctg agcgatactc ttttataata ctgaattcgg
      121 aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat aagacttctt
      181 tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag tattttatac
      241 gttatctaaa at
//
LOCUS       DASJST010000410          237 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.420, whole genome shotgun sequence.
ACCESSION   DASJST010000410 DASJST010000000
VERSION     DASJST010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.420"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>237
                     /locus_tag="U4B05_003124"
     CDS             <1..>237
                     /locus_tag="U4B05_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2052175.1"
                     /translation="NVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENS
                     YGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSN"
BASE COUNT           97 a           10 c           43 g           87 t
ORIGIN      
        1 agaatgttta tataggaaaa acagctttaa agattattct atctaatttg attagtaatg
       61 cggtaaaata tactgatgta aatggggtaa ttaatattgg gatagttaat gattggttat
      121 atatagagaa ttcatacggt aacaataaaa tttcgaatat ggataaaata tttgatgtta
      181 aatttgattt aaataaggaa aatagtaatg gattaggttt atatattgta agtaata
//
LOCUS       DASJST010000411          230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.421, whole genome shotgun sequence.
ACCESSION   DASJST010000411 DASJST010000000
VERSION     DASJST010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.421"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>230
                     /locus_tag="U4B05_003125"
     CDS             <1..>230
                     /locus_tag="U4B05_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2052176.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT           82 a           34 c           45 g           69 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc
//
LOCUS       DASJST010000412          230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.422, whole genome shotgun sequence.
ACCESSION   DASJST010000412 DASJST010000000
VERSION     DASJST010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.422"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           79 a           35 c           45 g           71 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac
//
LOCUS       DASJST010000413          224 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.423, whole genome shotgun sequence.
ACCESSION   DASJST010000413 DASJST010000000
VERSION     DASJST010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.423"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>224
                     /locus_tag="U4B05_003126"
     CDS             <1..>224
                     /locus_tag="U4B05_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN2052177.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DASJST010000414          223 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.424, whole genome shotgun sequence.
ACCESSION   DASJST010000414 DASJST010000000
VERSION     DASJST010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.424"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>223
                     /locus_tag="U4B05_003127"
     CDS             <1..>223
                     /locus_tag="U4B05_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2052178.1"
                     /translation="KLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQ"
BASE COUNT           70 a           45 c           44 g           64 t
ORIGIN      
        1 aaattggatg actcttcttc ctattgtgaa cactgtcgat cggttcgtat tatccgcaac
       61 ggttcctata cgacacgcac tcagatgttg aaagtcaaag aaaagttaac tattttagaa
      121 ttaaaacgta cccgttttct ctgccatgat tgtggaagga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc agg
//
LOCUS       DASJST010000415          222 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.425, whole genome shotgun sequence.
ACCESSION   DASJST010000415 DASJST010000000
VERSION     DASJST010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.425"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(2..>222)
                     /locus_tag="U4B05_003128"
     CDS             complement(2..>222)
                     /locus_tag="U4B05_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2052179.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           65 a           56 c           36 g           65 t
ORIGIN      
        1 ttcatttgtt tcctccttct tcgctttcag ccgagaacat tttgtaggtt cgatttgata
       61 cacccaacac actccctaaa aacgcgccaa aaccagtaat gatgacaaca cagatatctg
      121 tgtactgcca attgagcgct ttaccaacta accccacgaa agtagctagt gcgggaataa
      181 ttaccagtgc gaaccatttt agtacttcga acgttttatt at
//
LOCUS       DASJST010000416          218 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.426, whole genome shotgun sequence.
ACCESSION   DASJST010000416 DASJST010000000
VERSION     DASJST010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.426"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>218
                     /locus_tag="U4B05_003129"
     CDS             <1..>218
                     /locus_tag="U4B05_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2052180.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT           66 a           39 c           44 g           69 t
ORIGIN      
        1 tcttttattg cgaaaaattg tcaggtatct ttgaccaccg ttattcgtac gttgaaagag
       61 tttaaaagct acttaccaaa gcaatctaag aggattctcc ccagagtatt gatggttgat
      121 gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc agatggcgaa
      181 acaggacaat taattgatgt cttgcctacg cgtaaatt
//
LOCUS       DASJST010000417          217 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.427, whole genome shotgun sequence.
ACCESSION   DASJST010000417 DASJST010000000
VERSION     DASJST010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.427"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           87 a           35 c           33 g           62 t
ORIGIN      
        1 gtttggggtg cgctttaaac tcttctttat tattatctca cagcatgctt aaaaggtcta
       61 tggattaagt tatgtaaaaa agtgcctctt taaacgtcct aaaatccaaa caaggatagc
      121 ataatgtaca ctatcgttga tccagaaaac aggtaaataa attttgcaaa agcaataagt
      181 taaaaacatt acaaaaaaga caaggatatc acatcaa
//
LOCUS       DASJST010000418          215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.429, whole genome shotgun sequence.
ACCESSION   DASJST010000418 DASJST010000000
VERSION     DASJST010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.429"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..152)
                     /locus_tag="U4B05_003130"
     CDS             complement(<1..152)
                     /locus_tag="U4B05_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2052181.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCG"
BASE COUNT           60 a           42 c           36 g           77 t
ORIGIN      
        1 ccacagtgtt cacaatggga aggtgagtca tccaatctcc ctttaataaa ctgcgcttga
       61 acgcctttat attctttttc aatcagccat tcttcatcaa aaaataaatg tttatctgtt
      121 aatccgagta attttctagt atgattatcc attgagagac tcctttggta gggtgacgtt
      181 ttggtcgact aaattttacc aaaagtgagt ctctt
//
LOCUS       DASJST010000419          215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.430, whole genome shotgun sequence.
ACCESSION   DASJST010000419 DASJST010000000
VERSION     DASJST010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.430"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..213
                     /locus_tag="U4B05_003131"
     CDS             <1..213
                     /locus_tag="U4B05_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN2052182.1"
                     /translation="LFTKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESY
                     TMNKPSDNFSFRFAFRAKSKKTSPIK"
BASE COUNT           85 a           34 c           35 g           61 t
ORIGIN      
        1 ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat tgatgaatac
       61 gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa tatcaatttg
      121 ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt tgcgtttcga
      181 gcaaagtcaa aaaaaacgag tccaataaaa taaaa
//
LOCUS       DASJST010000420          212 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.431, whole genome shotgun sequence.
ACCESSION   DASJST010000420 DASJST010000000
VERSION     DASJST010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.431"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>212
                     /locus_tag="U4B05_003132"
     CDS             82..>212
                     /locus_tag="U4B05_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2052183.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     "
BASE COUNT           77 a           36 c           37 g           62 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat ac
//
LOCUS       DASJST010000421          212 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.432, whole genome shotgun sequence.
ACCESSION   DASJST010000421 DASJST010000000
VERSION     DASJST010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.432"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..>212
                     /locus_tag="U4B05_003133"
     CDS             83..>212
                     /locus_tag="U4B05_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2052184.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRI"
BASE COUNT           86 a           23 c           42 g           61 t
ORIGIN      
        1 ttgaaattaa ggtagattga caaacgagtg atcaaataga tatactacct tcaaacgacc
       61 gtttgaatgt gaggtgagat gaatggaaaa agtttgccaa gtgactatcg tacataaaga
      121 aaaagtaaat gtaataaaaa aaaaattgga tcaaaaagat ttttcaaact tacttgtgtt
      181 agggaaatgt ttcagcgatt cttctaggat ta
//
LOCUS       DASJST010000422          206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.denovo.434, whole genome shotgun sequence.
ACCESSION   DASJST010000422 DASJST010000000
VERSION     DASJST010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.denovo.434"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>206)
                     /locus_tag="U4B05_003134"
     CDS             complement(<1..>206)
                     /locus_tag="U4B05_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2052185.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DASJST010000423        12034 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.090, whole genome shotgun sequence.
ACCESSION   DASJST010000423 DASJST010000000
VERSION     DASJST010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.090"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..452
                     /locus_tag="U4B05_003135"
     CDS             <1..452
                     /locus_tag="U4B05_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN2052186.1"
                     /translation="LRNNWYVENELPIIGQALKTGQFVYAAGNGKTGWALKREYAEAA
                     AKVLVESDSPEILELSGNLTDYEELAKALERATGKELEIIEASDAAFVENLKEAGFPQ
                     EAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAALKEILDQE"
     gene            complement(703..2661)
                     /locus_tag="U4B05_003136"
     CDS             complement(703..2661)
                     /locus_tag="U4B05_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2052187.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2658..3530)
                     /locus_tag="U4B05_003137"
     CDS             complement(2658..3530)
                     /locus_tag="U4B05_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2052188.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3872..4744)
                     /locus_tag="U4B05_003138"
     CDS             complement(3872..4744)
                     /locus_tag="U4B05_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2052189.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4872..5441
                     /locus_tag="U4B05_003139"
     CDS             4872..5441
                     /locus_tag="U4B05_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2052190.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5508..6245
                     /locus_tag="U4B05_003140"
     CDS             5508..6245
                     /locus_tag="U4B05_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2052191.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            6476..7744
                     /locus_tag="U4B05_003141"
     CDS             6476..7744
                     /locus_tag="U4B05_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2052192.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            8231..8695
                     /locus_tag="U4B05_003142"
     CDS             8231..8695
                     /locus_tag="U4B05_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2052193.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8679..9308
                     /locus_tag="U4B05_003143"
     CDS             8679..9308
                     /locus_tag="U4B05_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2052194.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            9662..11140
                     /gene="msr(C)"
                     /locus_tag="U4B05_003144"
     CDS             9662..11140
                     /gene="msr(C)"
                     /locus_tag="U4B05_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2052195.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            11187..11633
                     /locus_tag="U4B05_003145"
     CDS             11187..11633
                     /locus_tag="U4B05_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2052196.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11832..>12034)
                     /locus_tag="U4B05_003146"
     CDS             complement(11832..>12034)
                     /locus_tag="U4B05_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2052197.1"
                     /translation="ATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRI
                     MLCQQELQWCQQVREQMQQFFE"
BASE COUNT         3851 a         2207 c         2439 g         3537 t
ORIGIN      
        1 ttttacggaa taattggtat gtagagaatg aattacctat cattgggcag gctttaaaaa
       61 ctggtcaatt cgtttatgct gcaggaaatg gaaaaacagg ttgggcattg aaacgtgaat
      121 atgcagaagc tgcagcaaaa gtactagtag aaagtgactc tccagaaatc ttagaattat
      181 ccggcaatct aactgactat gaagaattag ccaaggcatt agagcgtgcg acaggtaaag
      241 agttagaaat tattgaagca agtgatgcag cgtttgtcga aaatctaaaa gaagccggat
      301 tccctcaaga agcagctgac atgtttttag cgatccaaca tgatattaaa aaggatcagt
      361 tagctatttc ctctgatgat ttagaaaaag tattgggaaa accattaact tctcttgaag
      421 cagcattaaa agaaatattg gatcaggaat aatgtataaa gaggttgtga aaaagccgat
      481 tagttttgag tatgaagaaa aattttgtga aaaagctgtt ctgcatcaag taataagaag
      541 agtcaaataa aaaatagctc ttcatatgtt tcgtttagct ggacttatta ccgcggagtt
      601 ggcaggagaa cgccgttttt attcggaaaa aggagctgag acaagatttt tgtctcagct
      661 cctgtttgtt tatcgataaa aaagtctggg gaagacgatg gctcaggctt tgttggaaac
      721 agagccgatt ttatcatttg cttttacatc acgtgcgggt tcgatttcta agctttggac
      781 tttgtcgcca tttgtgaata caacgaccat atccgtgttt ttgcctgcag attgaatagc
      841 ggcaagatca actaaagcaa tcaattgacc tcttgcgact ttttgacctt cttctacata
      901 taaagtgaat ggttcgcctt ttaagtcaac agtgttgatg cccatatgaa gcaagacttc
      961 gataccagca tctgtttgga ttccgacagc atgtttcgtt gggaaaatgt ttgtgatcgt
     1021 accagcgatt ggagagaaga tttctccatt ttctggtaat acagcatagc cgtcacccat
     1081 cattttttcc gcgaatacat catctgaaac ttcagtgatt ggaatcagtt tcccatttgc
     1141 gatagcgtaa atatcttgtt cgatcgtttc accggcaaat gtttctgttt taaccacggt
     1201 gtttgtttgt gcaggtcgtg ctgcagcacc accatgtagt tttgccatct catctgcaac
     1261 gaattgtact tctgtaccta cgatgacttg cgcatttttt ccgtcgatga tcttcatacc
     1321 aggtacgcca gtggctttga ttttattttg atcgatcagg cctgtatctt tgacttgcaa
     1381 acgaaggcgt gtagtacagt tatcgatcga agttacattg tcggctccgc ctaatgcagc
     1441 gtagattttg cttgcttgtg ccgcatattt attgtctgaa ttagtcgttg cagtatcgtc
     1501 atctgaaaga tctgcttcct cgcggccagg agtcatcaga tggaattttt tgatagcaaa
     1561 gttaaagcca aagtagtaaa tcactgctgt tactaatcct tggacgatca acatcaatgg
     1621 ctgattcgca atcggatttt tcaaagatag gaagtaatcg acaaatccgg cactaaaggc
     1681 aaatcctgct gtccaatgga agaatgcact cactgctaaa gaaatcccag tgaaaattgc
     1741 atgcaagaca tataatggcc aagcaacgaa cataaatgaa aattcaagtg gttctgtcac
     1801 accagtaaag aatgaagcaa aagctgctgc tagcatcaag gaagctgttt ttgctttgtt
     1861 ttctggacgt gcatttctgt aaatagcata tgctcctgct ggaagcccga acatcatgat
     1921 tgggaaaaat ccagcttgat acattcctgt tacgcctttt gttccttcag aagccaaaaa
     1981 tttaccaata tcattgattc ctgctacgtc aaaccagaac acggagttca ttgcttgatg
     2041 caatcctgta gggatcagta aacggttgaa gaagccataa agacctgcac caccccagcc
     2101 taaactcaaa atgaatttag caaaagaaac taaagcagag taaaccggag gccatacgag
     2161 cagtagcact gcactgatca gcaacatgac aaaggcagcc aagattggca ctagacgctt
     2221 gccgctaaag aaagataaag ccatcggtaa ttttgcttcg tggaaacgat tataaagagc
     2281 tgccgcaatc aatccggcaa taataccaat aaatacgtta ctgatctgag caaaagatgg
     2341 atcaactgat tctggtttga tattcaataa agtagctact gaatcagttt ttaaaacatt
     2401 tactggaact tcaaatgcga ccaaacctgc caaggcagca gcacctgatt tgtccactga
     2461 catccctagt gctaacccaa cggcaaacag caaaggtaat tgacctaaaa ctgcgttacc
     2521 gccagcttgt aaaaaatgag cgacaatgtc gttactataa gatgcccacc agtttccgat
     2581 ccctactagt aaagcagcag caggtaagac cgcaactgga agttgaaggg aacgtcccat
     2641 tcgttgtaga tatgctttca atttttttcc tcctcatgat tgttaacatt tgtttgtacg
     2701 attgcgcttc ttaaacgctc gatatggatg gccaagtaac ccagttcttc tttcgtgatc
     2761 gcgagctggt attctttggt cagcaacaca cggatttttt tagaaatctt atagctttct
     2821 gggtattttc gtttgaccat ttctacgatt tccgtgtcta aaacagcata ttgttgcgca
     2881 tgggttcgtt gtagaagcag tcgtaaatga ttcaccagtc ttgaataatt cagtgaaaat
     2941 ggttctgaat ggatatctgt ttccaattct gattccacta aatggatgat gtcagataca
     3001 atcgtcactt cacggatact gcgatggtta ttgttccgac cgcttcgtcc gctgtgaata
     3061 tggattgcga tatatcctgc ttcgtcataa gtatatggaa tcccaagctc ttttgtcaga
     3121 tactctactg cccattgagc aatactgaat tcatcactgt aaagcacttc gatttctctt
     3181 aaaagtttgt tgcggatgac gataccgtta ttaatgttct ccgcagaaaa agcaatgtgg
     3241 tcggtcaaag cgatcaatac atgttcattc aacttttcca tcaaaacagt ttccgcatga
     3301 tcaatgatcg tttctgctgc aaataaaaag cgttcatcaa tctgattaag taaattttgc
     3361 aacttgattt gaccttctgg ttccatgaca aatatccgtt cgacatcacg atcaaaaatc
     3421 aaatcatttc tttttctgtc aaatccaatc ccttttccaa tagctacttt ttcctgtcca
     3481 ccgtcatcta ccaatactgc attttgatta aagatctttt tgattttcat gcgcgcaccc
     3541 tttttgaaga tttggttttc acgtttgcta gaccagttga ttgcggccaa acaaaaatag
     3601 acacgaaaaa cctcctaggg aaaccatgtt cctcagagat ttttcatgcc tagtatgatc
     3661 tagtcacatg aaacgaatta ttgttaacgt aatcaatttt atatagacag ttatgttttg
     3721 tcaattcttt ttttataatt atagtaattt actggaatta tcagaaataa aaggaagaaa
     3781 taaattaaga aacttatgtt atttaatcgt tggatctttt gcttatctta ttcgttgtta
     3841 aaaagctgaa agcgaaggaa aaaaactcta cttaactgta tttctccgtt aggacatcta
     3901 aaaatttacg agcagcagga gaaagaggct ggtctttttt ccaaatgaga ttcagtgaag
     3961 cttgtaatgg cggatcgagt gggatgaata ttttttccgt cccataagta ttgatgatcc
     4021 catctagagc taatgcacca aaagtcccgt tatcgaccat caaagaagca ttgtacaata
     4081 agttatatgt tccgacgatc gataactgat ctatgctgaa acctagccat tcagctagtt
     4141 gactgtccac tgacgtttgc cgggaaatga tcaaaggaac agttttcagt tcctgcggat
     4201 ggatgacggt ttgctttgct aaaggatgat ttcgatgtat caaaagtccc caacgatctt
     4261 gggctggcag tcgaaatgac tggtattttt gtttgtttgt cggttcgatt acgagtccaa
     4321 aatcaagtaa tccgctatcc agtttgtcta tgatatcatc tgcattcccg ctatataaat
     4381 gaaactggat atcaggatgc gccttgatca acgaatgaag gctatcagaa atgaaagaga
     4441 ttgctttagt ttcgcctccg ccaatgtaga tttctccaga gatgattgcg tcatttgtta
     4501 aattgttcgc cgttttttct accaacgaca agatttcttt tccttttgaa taaagatatc
     4561 gaccagaatc cgtcaactgt atctctcggg agccccgagt gaaaagtgtc acaccaagtt
     4621 cttcttctaa atctttcagt tgttttgaga gagtaggttg cgtgatatgc agagattttg
     4681 ctgctcggct gatatttttt tctctagcta ctgctaaaaa atacgtcagt atacgaatat
     4741 ccattttttc cctcctgttt ttaaaaaagc ataccactaa gtatagttaa aaggaatagt
     4801 taagtatgtt ttataagtat tagacatgat aagccgtccg ttataaagta aggataaaag
     4861 gagggagcaa aatgacaagc ctcatcgaaa aacaaatcgt caataaagag attcgagtaa
     4921 cggatccgct ctttgaagtg atccatcaga tccaagctga aaacgaagag cctttagcag
     4981 cgctgaatac tgggtaccat gaaccagcag atatccgcaa acggctagaa aatatcatta
     5041 gtgacaaagt agatgatacg gtgactgttc tgctgccttt ttatacagat ttcggcaaac
     5101 atattagtat agggaaaaat gtctttatca atcgtcaagt gatgttcgtt gacttaggtg
     5161 gcatctgttt agaagattca gtattgattg gtcctcgtgt caacctcatc acggtgaacc
     5221 atctaaccga tccaaaagaa cgaagaggct tgttagtaaa gccaatccac atcaagaaaa
     5281 atgcttggat cggagcagga gcaactattc tgccaggcgt aacgattggc gagaatgcca
     5341 ttgttgcagc gaatgcaact gttacgaaag atgtcccaga caataccatc gttgcaggaa
     5401 tccctgccaa aatcgtcaaa cctgtcgaac ggattatttg aacagtaaga tgaaataaga
     5461 gatagtgagt ataagcaaga gatttttcaa aacaggagga gaatagaatg aatgatttat
     5521 cagaaaaagt gattgtcatc atgggcgctt caagcggaat cggtgaagca acaacgaagc
     5581 tattagcaga aaaaggggct aaactagtta tagctgcacg ccgagaagat cgcctaaaag
     5641 caataaaaga atctttacca gaagctgagc tatatattca aacagcagat gtaagagatt
     5701 ttgcacaagt ccaagcagtt atcgatttag cgatggaaaa gttcggtcga atcgatgtat
     5761 tgtacaataa tgcaggaatc atgccaaccg ctccattagt tgaaggtcac cgtgatgaat
     5821 ggcaaaatat gcttgatatc aacatcatgg gtgtgttaaa tggaatttcc gccgtacttc
     5881 caatcatgga aaaacaaaaa tctgggcata tcatttcaac tgattctgtg gcgggacatg
     5941 ttgtttatcc tgattccgcc gtttattgcg gaacaaaatt tgctgtccgt gcaatcatgg
     6001 aaggattacg tcaagagcaa cggcaaaata acatcaaatc tacgatcatc tctccaggag
     6061 ctgttcaaac tgaattatat caaaccatca gcgacaaaaa ggctgctctt gaacttcatg
     6121 aagcgcaaaa agaatgggga ttgacctccg aagatatagc ttctgctgta gcatttgcga
     6181 tagaaacgcc agaccggatg tctgtcagta acatgattat ccgtccgacg acacaggaag
     6241 tataatccat aacggataat atccaaagag aattacttgt taaatagtta ttcaaacgac
     6301 catcaattta aggcgtgaat ggagaagaat attcttcatt tacgtctttt tatgtagaaa
     6361 aagcaaattt atcaaaaaga aacaaatgtt atttaaatat ggacattgac ttgcttttac
     6421 ttttttagtt acactgttat ttaggaaaag tttcataatt caaaggagaa aaaagatgaa
     6481 agcaaagaat ttattattta gcgttattct tttaggaaat ctagcagttg ccaacacaac
     6541 agtgctagct gaagaatcga cagaaactgt cgcaactcag acatcgagtt cttcagaaac
     6601 aggaattgaa aacgatacag agagtacttt gcagtcagct actgtctcag aaaatacaat
     6661 ttcaaccgaa gaaccaacaa ctgaaacaac agaacaaaca caaaccgaag acagtacatc
     6721 agcagaagct cagaacaatc cagagaatgc agaagaaatc acgaaccaat tgacagatgg
     6781 gcaaacattg acacataccc atcaagcaga tttgtctgaa ttcgaaaact atactggcta
     6841 tgctttttat gctcgctcat ctgctaattc tcaacaagcg ttcattgata gtattgcctc
     6901 aactgcacaa agtttggcaa gtgccaatga cttatatgca tcagtcatga ttgctcaagc
     6961 catcgttgaa agtggctggg ggaacagtac tttggcttct gcccctaatt acaatctatt
     7021 tgggattaaa ggaagctata atggccaatc tgttacgatg cctacttcgg aatatgtgaa
     7081 tgggcaatgg attacggtga atgccgcttt tagaaaatat ccttcgtaca aagaatcatt
     7141 gcaagataac gtaacggtat tgaagacgac ttcttttcag ccaggcgttt actactattc
     7201 cggtgcatgg aaaagcaata cgaactctta caaagatgct acggcttggc taacaggtcg
     7261 ttatgccact gcgcctaatt atggttctac attgaacaat gtaatcgaaa cctataatct
     7321 gacacagtat gatacagcac caacttcaac ttctccgatg tatcgtctgt ataaccgtca
     7381 tacaggagaa catttatata cattgaatgc cggagaaaaa aattatctgc caactgtagg
     7441 ctgggaatat gaagggattg cttggcaagc accaaacagc ggacaacctg tctaccgttt
     7501 atacaatcct tacagtggag accatcatta cacaatggca caatctgaga tcaactcttt
     7561 aacaaaaatc ggttggcgtt atgaaggtct aagtttttat tctggtggtt ccaagccaat
     7621 ttatcgacta tttaatccaa atgaaaaaac tggtacccat cactatacct tgagtgctaa
     7681 ggaaagagat tggctaacac cgatgggctg gagatatgaa ggaataggat tttatggtta
     7741 ttaaaaagag gtagtgaaaa agctgccctg catcaagtag taagaacagc caaacaaaaa
     7801 cagattttca tgttttttgt ttggttgaac ttattaccgc agatgcaagt ttttgaacac
     7861 cgtttactca aaaaaggttg tgacaacatc ttgtcataac ttttttatta ctgtttaagc
     7921 atactgatcc aatttctaat attcgacggg attcttgcaa aaggcgattt cggaaatttt
     7981 tggcggtgta aaattttcta ctgcagaaga gctatcttct atagtttgat aagaatcaat
     8041 aatcttggtt ttagcgttgt gctttcttta tcattttttc cgttcattct acaaaaaatt
     8101 tcttttctct atacttttct attgagagcg ttttttttat tacacagctg attctttgac
     8161 ttttttcttt ttttattcta ttattgacaa gtcaatcgtt gacgagtcaa ttattagaaa
     8221 ggaggaaatt atgtctttag aagagattag tcacctttta tatcaaatca aattagccga
     8281 tcaaaaaatc gtccagatct ttgaagaaaa tatcggtata agtcttacta gatacgagat
     8341 catgatgaga ctgaaagaag aaaatccatt gctacaaagc agtttgcaag aaaagttgca
     8401 gattgatcaa ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc
     8461 gcgtcagaga aatcctcaga acaaccggga agtgtatgta gcgctaacag aaaaagccca
     8521 gaagaaaatc gagcattgtg aaatggacca taaagataca ggttccgtgc tatccccggc
     8581 tttcagcgat gtagaaatga aacaatttct aacactttta gataaattga atacccaact
     8641 ttgcaatata acaacagaaa cgaaagaggt taataataat ggaacaaaaa aatgattttt
     8701 caacaattat gactggtaga agatccgttc gcgtatatga tgaaacatac aaaatcccac
     8761 acgaagaaat gctggaaatg atcaaagaag cgacgactgc accttcttct gtcaatatgc
     8821 aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga
     8881 tccgtttcaa tacgagacaa aatgatactt cttctgcaat ggtcatgatt tttggcgata
     8941 tgcaatgtta cgaatatggc gaagaaatct ataaccaagc ttacgaatca gggaagatgc
     9001 caaaagaagt cagagatcaa caattagctg cgatcattcc ttattacaag agtttttcaa
     9061 gagaagaaat gaacgatgta gtcaaagtcg attctagctt agcggctatg caattcatgt
     9121 tagttgcgcg tgatcatggc tatgaaacaa atccaattgg tggagtcgaa gcagatcagt
     9181 tggcagaggc atttggtttg gataaagacc gttatgttcc agttattatt ttatcaatag
     9241 gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc accagaaaag atcactacgt
     9301 tcgaataaaa ggttttcagt tactattcag tagctattgc tttttttgaa tcgtgtgtgg
     9361 taacctaata aaaaaggagg tcggaaaaat gactgcatcg atgaaattac gtttcgaact
     9421 tttgaataac aactaaataa attcaaaaga catgtataaa caacaatttc atggatggag
     9481 tatgtccatt ttttcgaacg acagtagaat aggtgaaagc tatgacagat ttggtgattt
     9541 agtcatagtt atttcgttca gagtaggaaa agatgtgatg cagttttttt gcaaacagat
     9601 ttttctgctc ttttttagta tcgataattg ttgttttaca tgaaatagga gtgtaaaaca
     9661 tatggaaaat ttagcagtaa atataacaaa tctacaagtc agctttggca accagctaga
     9721 actatctatt gattctcttc gtgtctatca gcaagatcgt atagggatca taggagaaaa
     9781 tggtgtcggt aaatcgactt tgctcaaact aatagccggt gaactttttc ccgatcatgg
     9841 gaaaatccaa acagagatca ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc
     9901 aaaggaccta aatttggaat tagccagtca tttccagtta agactggaag aaacttcaga
     9961 gcggaaatgg agcggagggg aagaacgaaa gatcgagttg atacgtcttc tttcttctta
    10021 tgaacaaggg atgcttctag acgagccaac aacccatcta gatagaaaaa gtattgatcg
    10081 actgattgaa gagcttcgtt attattatgg tacgctggtt tttgttagtc atgatcgcta
    10141 ttttctagat gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt
    10201 ttcggggaat tatagtgcct atctcactca aaaggaattg gagaaaaaga ctcagctacg
    10261 agaagcagag tcgatcatga aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa
    10321 aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagacc
    10381 ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc
    10441 gaaaacatta gagagaagac tccaaaaaat aggagaaaca accaagccgc agcagatgaa
    10501 acaaatccgt tttccagtac caaaatctct tgaactccac agccgctatc ccatcatggg
    10561 acaaaatgtc caattggaaa gaagtgggag aacattactg gtaaatggtg attttcagtt
    10621 ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt
    10681 agaacatatc cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa gctttcaagt
    10741 atatcagcaa aagggttatc aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag
    10801 acaaacagag ttttcagaat cgcttgtccg tagtttgctg aatcacttag ggtttgctca
    10861 ggaaactttg acgaaaccgt tatgtacatt aagtggggga gaagcgaccc gtctgacgat
    10921 tgctttgctt tttactaagc caagtaatgt gttgctgtta gatgaaccga ctaattttat
    10981 tgatatggca acgatcgaag ctttagagaa gctaatgcaa atatatccgg gaacgatttt
    11041 gtttacttct catgattctt actttgtcga gcgtacggct gatgaagttt atgaaataaa
    11101 agggcagaaa ataaaaaaag tacttacgag aaatttttaa atgtgctacg atgattttcg
    11161 tgacaaattt aaaggagtgg tacagattgc ctacaaataa aaaagaagga ataattttta
    11221 caacaatgat gtgttttttg atggtcttgg ggatgagcgc gtataattta tggctgcatc
    11281 aagatcttca tttatcaagt ttgttgattg gtctagtgcc tggattcgtg gtggctttca
    11341 ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat tgcctttaaa ctaccagtaa
    11401 atccagaaag caaggtccaa atgatcttaa ctatttcatg cttgatggtt ttaggaatgg
    11461 tgacatgtat gtctttattc ggtgtgatca tggaacaagg aatacctgat caattattct
    11521 ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc tttaccgttg cagttgatca
    11581 tcgttggccc gctttcaaga agtgtgctaa aagcaattca aagtaatgaa tgatggaaca
    11641 ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata gtctcttgaa tgcagttcta
    11701 gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt cggatcgctt tttcttttcc
    11761 aactgtattg ctcttttatt gaagataatt tgctcttcaa tagatatttc tagaaaggtt
    11821 ctgtttatcc cttactcaaa aaactgctgc atttgttctc ttacttgctg acaccattgg
    11881 agttcctgct gacaaagcat aattctgcgc tggatcacaa cataacgtcc aaattcttgt
    11941 ttcttgtcta gcgagaggtc attcaatttt ttttgatttt tttgcatgat tttgttcagc
    12001 tgttgttctc tagtgaatag cagtccagta gctg
//
LOCUS       DASJST010000424         8233 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.119, whole genome shotgun sequence.
ACCESSION   DASJST010000424 DASJST010000000
VERSION     DASJST010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.119"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..367
                     /locus_tag="U4B05_003147"
     CDS             <1..367
                     /locus_tag="U4B05_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2052198.1"
                     /translation="FLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHG
                     KVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTL
                     EKTKILGQTLSRFFTILPK"
     gene            625..2127
                     /gene="eat(A)"
                     /locus_tag="U4B05_003148"
     CDS             625..2127
                     /gene="eat(A)"
                     /locus_tag="U4B05_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2052199.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2155..2721
                     /locus_tag="U4B05_003149"
     CDS             2155..2721
                     /locus_tag="U4B05_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2052200.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            2932..3993
                     /locus_tag="U4B05_003150"
     CDS             2932..3993
                     /locus_tag="U4B05_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2052201.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3996..4892
                     /gene="murQ"
                     /locus_tag="U4B05_003151"
     CDS             3996..4892
                     /gene="murQ"
                     /locus_tag="U4B05_003151"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN2052202.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4906..6888
                     /locus_tag="U4B05_003152"
     CDS             4906..6888
                     /locus_tag="U4B05_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2052203.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6953..7399)
                     /gene="nrdI"
                     /locus_tag="U4B05_003153"
     CDS             complement(6953..7399)
                     /gene="nrdI"
                     /locus_tag="U4B05_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN2052204.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7610..>8233
                     /locus_tag="U4B05_003154"
     CDS             7610..>8233
                     /locus_tag="U4B05_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2052205.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSC"
BASE COUNT         2683 a         1434 c         1840 g         2276 t
ORIGIN      
        1 ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt
       61 ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca
      121 aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc
      181 aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc
      241 aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca
      301 aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc
      361 gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg
      421 agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt
      481 gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg
      541 gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
      601 tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc
      661 tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg
      721 aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa
      781 aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa
      841 cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag
      901 atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga
      961 gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg
     1021 gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga
     1081 aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac
     1141 cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa
     1201 ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa
     1261 atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa
     1321 aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc
     1381 gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg
     1441 aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta
     1501 ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag
     1561 cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt
     1621 cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag
     1681 tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac
     1741 cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg
     1801 ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga
     1861 aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa
     1921 cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat
     1981 gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac
     2041 gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact
     2101 gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt
     2161 aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa
     2221 acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa
     2281 ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg
     2341 aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt
     2401 ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat
     2461 ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc
     2521 ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc
     2581 atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc
     2641 aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca
     2701 gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca
     2761 ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc
     2821 aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga
     2881 tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc
     2941 ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg
     3001 gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg
     3061 gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg
     3121 atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg
     3181 cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag
     3241 attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa
     3301 gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat
     3361 tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg
     3421 ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc
     3481 ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc
     3541 gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca
     3601 gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg
     3661 gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac
     3721 gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca
     3781 gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg
     3841 cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat
     3901 gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg
     3961 aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc
     4021 taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag
     4081 ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga
     4141 caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga
     4201 tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac
     4261 cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa
     4321 tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca
     4381 agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat
     4441 atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt
     4501 gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc
     4561 cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga
     4621 atatgatttc tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg
     4681 atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga
     4741 cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc
     4801 tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg
     4861 aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa
     4921 ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta
     4981 gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta
     5041 aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt
     5101 caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca
     5161 ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa ttcatcagca
     5221 tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa
     5281 caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg
     5341 attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta
     5401 gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc
     5461 ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt
     5521 tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg
     5581 aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt
     5641 gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat
     5701 aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc
     5761 ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc
     5821 ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca
     5881 ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa
     5941 atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga
     6001 caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa
     6061 atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga
     6121 gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg
     6181 gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct
     6241 ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct
     6301 tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa
     6361 gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa
     6421 acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta
     6481 gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa
     6541 ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg
     6601 aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc
     6661 aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg
     6721 acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa
     6781 acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa
     6841 acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac
     6901 aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat
     6961 tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat
     7021 aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact
     7081 gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt
     7141 catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac
     7201 catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa
     7261 aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat
     7321 gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg
     7381 atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat agcatctttt
     7441 ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt ataatgagaa
     7501 aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa aataaaaaag
     7561 gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca tggatacaga
     7621 gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg gttcagcgat
     7681 tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg gcccagctat
     7741 tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga tcaataatat
     7801 caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag aaggttttgg
     7861 ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt taggcaacgt
     7921 agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg
     7981 gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg catggctagt
     8041 gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct gtaaattagt
     8101 gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac aattcattga
     8161 taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt tttccagtca
     8221 agtattgagt tgc
//
LOCUS       DASJST010000425         4238 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.190, whole genome shotgun sequence.
ACCESSION   DASJST010000425 DASJST010000000
VERSION     DASJST010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.190"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            117..614
                     /gene="dfrG"
                     /locus_tag="U4B05_003155"
     CDS             117..614
                     /gene="dfrG"
                     /locus_tag="U4B05_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN2052206.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(686..2638)
                     /locus_tag="U4B05_003156"
     CDS             complement(686..2638)
                     /locus_tag="U4B05_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2052207.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2645..2881)
                     /locus_tag="U4B05_003157"
     CDS             complement(2645..2881)
                     /locus_tag="U4B05_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052208.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3283..3573
                     /locus_tag="U4B05_003158"
     CDS             3283..3573
                     /locus_tag="U4B05_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2052209.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1332 a          719 c          741 g         1446 t
ORIGIN      
        1 taatataact tatagaaaaa attgcttatt caactaacgg gcaggataat gagaaaaggt
       61 tttttgatag aaataagaaa ttagatggta aattagttaa aaaggctgtg ttataaatga
      121 aagtttcttt gattgctgcg atggataaga atagagtgat tggcaaagag aatgacattc
      181 cttggaggat tcccaaggac tgggaatatg ttaaaaatac tacaaaggga catccgataa
      241 tattaggtag gaagaacctt gaatcaatcg gaagagcctt acctgacaga agaaatatta
      301 ttctgacgag agataagggg tttaccttta atggttgtga aattgttcat tcaatagaag
      361 atgtttttga gttatgtaaa aacgaagaag aaatttttat tttcggagga gaacagattt
      421 ataatttgtt tttcccttat gttgagaaaa tgtacatcac aaaaatacat catgaattcg
      481 aaggagatac tttttttcca gaagtgaatt atgaggaatg gaatgaggta tttgcccaaa
      541 aagggataaa gaatgataaa aatccgtata actactattt tcatgtatat gaaagaaaaa
      601 acttattgag ttaacgggtg cgttaattga agaagaacag taaagataaa gcacaagagc
      661 ttgggattac tccaagctct ttttgtcatt tgaaataaat tatatctttc caatcaaatt
      721 gtttatctgt aatccctaat ctttgagcag gtgtcaaaac ctttttatcc gctgatttaa
      781 aagggcgaca aaagttgtag taagttctta gaattgtaat cgcaaattga gcatacttcg
      841 gattgaagtt agcgtaaata tagctcttct tatcaccacg agctgttgtt aatggtcttt
      901 caagagatga tatataacgt cttatctgtt gaataaagct attggtcgaa tggtcattaa
      961 catttaatag catctttgcg atttcgtttg gttccagagc agataaatcc gttctacaat
     1021 caactgaata aaatccttta tccttagtgg ctaatggatg tttgataggg ttatcggcat
     1081 atttgcgata tctttctcca tctttactaa tagcctcttc gtggaattga tgacttgtta
     1141 gtaattcagt tagataatga gatgccaaag tccgtagaaa ctttgttttg aatcctctga
     1201 tatctcccca atcgagcagt tctgcttttg cttgtccgaa ttcttttaag cattgttttc
     1261 tgcttttcgt cttgttaact tggcaaataa aatgatgtgc atcgctcaac cttaattctt
     1321 tcgtgaatac tctataaaag gcgtttctaa tagagaagtc atcatcacta atcattcgcc
     1381 attcgtcggc attaaccatt tgtttaatta accaatagtg agcagttgta gtataaggag
     1441 catcaacatt taaaccatcg acaaatctac tcctattagt aattttccct aattcgtgaa
     1501 gataagcatt cctattttcg ctatcgttag cggatggttc ttgtggataa taagaccaat
     1561 ctaacctatc atttttacga ctaaaagtat tgagatggtc ttctttgaat tttcttgtat
     1621 cttcatttaa ttcatccatt gaaatattcc aatcataggc aacatcagaa cggaacacat
     1681 atcttgataa gacctcagca gatacaacta tatacgtttg catattaagg tcttcaaacc
     1741 ccgtatattt ctttgaacct tgtcctttct tacggacgtt atttaggtag tagtgcatct
     1801 tgtccgtatt aagccacatc tcgttaaatt tctttgtttg aaggggttgt gtctcatatc
     1861 gttctaaaaa ctccaaacaa cgcctataca gccactccag cttatgatag taggttccaa
     1921 ctccaattcc taaaatatca caagtacgac tgactgaaac ccttccaaca accatcttag
     1981 cgaacattgg aagaatggtg ttcttttgtt ggtgataagt agtggtttct tctctttttg
     2041 gtagaacatt agtgaacttc ttacacgcct tacattgata gcgttgagat ttgcccctac
     2101 ttttccccct tttgtaaaat tgcttcggct cattgaatgg ggttgattct tcttccgagc
     2161 agccttcttt atgaaattga taatctggct caacatcttg gatgctattg atttctataa
     2221 ggcgtttaat ttcttcaaca acagaccaat tagataaagg ggttactgta cagttttgcg
     2281 atacacctct gccaatagga ttgtcattgc agtacatacc tttatgacct ttatctttgg
     2341 aactaccagt cattttatat cgacttggtt tcccttttac atctttgaat ttgtgctgtt
     2401 ctttcccaaa attacaacaa aacggattga tgcagtaatt atattgaatg ggatattcag
     2461 aaccgttcca tttaaaagac gttggcaaga acaacatttt gttatacctt gttgcaaatc
     2521 gttttggagt taataaatca tattgtttag ccctatcttt taattcttcg tttgaaatcg
     2581 gaattttaac atctacaatt tcatcttcct tcgtggctaa tcttttaagc ttggtcattg
     2641 tctatcaacc cactctatct ttgataccca ttttttcaac aatccgctta ttacttcgct
     2701 tgttggcaat ccattcaata aacttcttat cgtctaaaat gttcaataag aattcatcca
     2761 ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg cttaactatc acccttactt
     2821 gctctgatat ttcccaatcc acagacttag cgttcttatt ttttggctca agaaattgca
     2881 taggaaaatg cacccctttt tgcttagtaa attgtaacca ttttaacaca ccaatttgca
     2941 ttcctacaat aattatttca cgttcgcatt tttatataca ttttaactta cattttgtgg
     3001 tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg agtatcaaat tcagttgcaa
     3061 tttgatactc atttaggttt acattttcac tttttgtact ccaaaactga actacttaaa
     3121 tctatactgc tgcctctttt ttttagtaca tgtccttcat cttcgcagga agtgccaata
     3181 tgattttaag aaaggtaata gacaaaaagt caaaaagata aaacagagtt tcttctttac
     3241 aaagggaatg tatgttcgta taatggtatt gaggtgaagg ttatggagaa aaaatatgga
     3301 acagtagata agatccgtac tttgcaagtt agtgccagac cgttgatccg tttcagttta
     3361 aatggggtca attgtttgat tgcttcccat agtttgaact tcctagctga agtagacgaa
     3421 gggatgaagt tagtcgttac aggttattat aataatagaa agcaatttgt tgtgagatcc
     3481 tatcatgtgt tagggaaacc caaaattgtg gtagaatatg agaaaagtct atacccaaga
     3541 aaaaaggtcc gacaagatga aagaataaac tagaaaacaa gcggctgaga caaaaatgtc
     3601 ctagccgctt gttttggaaa gatggatgca gacgatggga tttgaaccca cacgagcata
     3661 cgctcacaag aacctcaatc tatatataac tacatgtatt taagtatttg tgatgaatca
     3721 ctaaactttc caaactcatt tgagggatat tgcttaaaga ttgaacagat caattcgaag
     3781 ccctattagt attcgctcaa tggggggatc agaggccata aaaagagact agtcattact
     3841 agtctcaggt agtggaattg taggatttta aacgttcctg gagagtaccg gtatgtaact
     3901 cgaagaggtg attatcatag tcgtaaaagt aaattgaaga agcctcgcct ttcacacgag
     3961 aacgtcctgg ttcgacagtt aaacctaaca tttggatttt tgagacaaac gaatctatat
     4021 cttgttcgtc aattttaaaa gcaatatgat tgtatgtctt tggcagttta ttcgacgaat
     4081 tctgcattac tgcaatccat aggtccttaa ctaagaaaaa tttttctgga tataatgaaa
     4141 atttttttgt tgactatcgt agatttcttt tgcatcaaat aaggtattta acaattaagt
     4201 atttaaaaga taacttttaa gttatcttta agtaatgc
//
LOCUS       DASJST010000426         3321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.210, whole genome shotgun sequence.
ACCESSION   DASJST010000426 DASJST010000000
VERSION     DASJST010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.210"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            246..1214
                     /locus_tag="U4B05_003159"
     CDS             246..1214
                     /locus_tag="U4B05_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN2052210.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1207..2238
                     /gene="vanA"
                     /locus_tag="U4B05_003160"
     CDS             1207..2238
                     /gene="vanA"
                     /locus_tag="U4B05_003160"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN2052211.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2244..2852
                     /locus_tag="U4B05_003161"
     CDS             2244..2852
                     /locus_tag="U4B05_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN2052212.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          993 a          630 c          804 g          894 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc ataatttttt aggaaaatct
       61 caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt
      121 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
      181 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      241 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      301 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      361 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      421 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      481 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      541 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      601 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      661 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      721 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      781 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      841 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      901 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      961 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
     1021 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
     1081 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1141 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1201 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1261 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1321 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1381 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1441 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1501 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1561 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1621 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1681 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1741 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1801 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1861 gtattgggaa acagtgccgc gttagctgtt ggcgaggtgg accaaatcag gctgcagtac
     1921 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1981 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     2041 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     2101 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     2161 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2221 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2281 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2341 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2401 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2461 atcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2521 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2581 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2641 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2701 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2761 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2821 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     2881 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     2941 tatagtagat agtattgaag atgtaaggca gagcgatatt gcggtcatta tctgcgtgcg
     3001 ctgcggcaag atagcctgat aataagactg atcgcataga ggggtggtat ttcacaccgc
     3061 ccattgtcaa caggcagttc agcctcgtta aattcagcat gggtatcact tatgaaaatt
     3121 catctacatt ggtgataata gtaaatccag tagggcgaaa taattgactg taatttacgg
     3181 ggcaaaacgg cacaatctca aacgagattg tgccgtttaa ggggaagatt ctagaaatat
     3241 ttcatacttc caactatata gttaaggagg agactgaaaa tgggctcttt gtcaaatgat
     3301 gttggtaaag agaaatctta g
//
LOCUS       DASJST010000427         2556 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.238, whole genome shotgun sequence.
ACCESSION   DASJST010000427 DASJST010000000
VERSION     DASJST010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.238"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..458
                     /locus_tag="U4B05_003162"
     CDS             <1..458
                     /locus_tag="U4B05_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2052213.1"
                     /translation="LFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRDK
                     KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKG
                     RLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            672..1367
                     /gene="vanR"
                     /locus_tag="U4B05_003163"
     CDS             672..1367
                     /gene="vanR"
                     /locus_tag="U4B05_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN2052214.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1345..2499
                     /locus_tag="U4B05_003164"
     CDS             1345..2499
                     /locus_tag="U4B05_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2052215.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          881 a          454 c          536 g          685 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacct acaggaagat gacatcattt
       61 atgttacaga cttaactcga atcactcgta gtacacaaga tctatttgaa ttaatcgata
      121 acatacgaga taaaaaggca agtttaaaat cactaaaaga tacatggctt gatttatcag
      181 aagataatcc atacagccaa ttcttaatta ctgtaatggc tggtgttaac caattagagc
      241 gagatcttat tcggatgaga caacgtgaag ggattgaatt ggctaagaaa gaaggaaagt
      301 ttaaaggtcg attaaagaag tatcataaaa atcacgcagg aatgaattat gcggtaaagc
      361 tatataaaga aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg
      421 cttcattata caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg
      481 gcaatatttt taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg
      541 aaaagccctt tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt
      601 aaatgcttat aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa
      661 gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt
      721 tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag
      781 aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc
      841 ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta
      901 tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg
      961 atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt
     1021 tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact
     1081 ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc
     1141 ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta
     1201 gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca
     1261 ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga
     1321 aatatataaa aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat
     1381 tccaaactag aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta
     1441 ttcgtgttgt atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt
     1501 ttggaaaaca aatatgactt aaatcacctg gacgcgatga aattatatca atattccata
     1561 cggaacaata tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt
     1621 cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta
     1681 cttattcaga acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa
     1741 aagctcaaca cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa
     1801 caaagaaaaa atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc
     1861 attatcggtt atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca
     1921 aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt
     1981 tttgagatta cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta
     2041 tactatatgc tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa
     2101 caggcggtta ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg
     2161 agagtcttta acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt
     2221 gacattaccg cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc
     2281 atcccaaaag ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt
     2341 tcttccgata cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag
     2401 catggagggc agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt
     2461 ccagcgatgc cagacttggt tgataaaagg aggtcctaag agatgtatat aattttggct
     2521 ctttgtcaaa tgatgttggt aaagagaaat cttaga
//
LOCUS       DASJST010000428         2315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.245, whole genome shotgun sequence.
ACCESSION   DASJST010000428 DASJST010000000
VERSION     DASJST010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.245"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            23..1261
                     /locus_tag="U4B05_003165"
     CDS             23..1261
                     /locus_tag="U4B05_003165"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2052216.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1488..1907
                     /locus_tag="U4B05_003166"
     CDS             1488..1907
                     /locus_tag="U4B05_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2052217.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1912..2151
                     /locus_tag="U4B05_003167"
     CDS             1912..2151
                     /locus_tag="U4B05_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2052218.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT          730 a          318 c          434 g          833 t
ORIGIN      
        1 ttaaataaaa aggagagatt ttatgcaagc agaaaaaaaa ctttttaatc gtgattttat
       61 acttgttgtt ataggacaga taatttcttt atttggaaac gctatcctac gatttgcatt
      121 accgtactat ttgttaaaag aaactggttc atcagctttg tttggtattg ctacagcctg
      181 ctctttttta cctatggtta ttttgtcgct ggtaggtggt attattgctg acaggacaaa
      241 taaaagaaac attatggtca tactagattt tgtaacagca atcataattt tagtatttta
      301 tttgtcattg ggaaaaattc ctatcatacc tttgtttatt gttgctttga tgttacttta
      361 tggtatttct ggtgcatatc aaccagcggt acaagcaagt attcctctat tggtatcatc
      421 agataaactt accgtcggta atgctataat aaatcaagta aatactttag caaactttat
      481 cggaccagct ataagcggag tagtttttca attttatggt atcaatccca ttttaattgt
      541 tagtattata tgttttactt tttcagccat tatggaaatt tttatccata ttccacacca
      601 aaaaagacct cgtgaagtag gtgtaattgc agttgctaaa aacgatttaa aagatagtta
      661 tacctttttg aaaaaggaga aacccatttt tatatccgta atctttttgg tttgcatttt
      721 caatttggtt ttaagtgctg ttttaattgt tggtctgcct atcttaattt ctgaagtttt
      781 aaaaatgcca gatacactat atggtttttc acaatcagca ttatcattag gtggtttatg
      841 tggtggtatt ttaacagctc ttgttgcttc aaagttaaaa ctaaaaaacc tttatacctt
      901 gttacttatt tgttctgttt ctgttttact tatgggaatt tcgctatttt tagaattgcc
      961 tgcaatagtt tcctactggg tagttacagt gatgtgcttt gtggctatgg gtgtatctac
     1021 tatgtttgtt gttcaaattt acactcttgt tcagacacaa acaccaccgc aattagtagg
     1081 aaagataatg gctacgctgg tagcagttgc tatgtgcggt caacctattg gacaaatggt
     1141 ttacggtatt ttattcgatg tatttataca agaaacatgg gttgttatgt ttgtagcttc
     1201 tattgcttca atcatgattt caatatattc aaaaagaatt tttcaaaagt tagagatata
     1261 atacaagggg ttaaatagta tgttaggcta aaaacaatat ataaaagaaa atttgaaatc
     1321 tgctaaaagg atagtgttat aaaagaaaga gtaatattgc agtatttaag gctcgcacaa
     1381 atatatgttt tgtgtgggct tttttcttac catttttctg tatttctaaa gggtataaac
     1441 ggtattccgt ttatatattt cagcattagc agaaaggggg tgagattatg aggacatctt
     1501 ctttcgagca tatcgtcaga ttacagttca attctttaat gctgattgtc attaagaaca
     1561 aagtgaaaag tcgaaacaga cagcttgcaa gacattctaa acgtgaagta ttgttttgtg
     1621 aaatagccga aacgaaacaa gttgaacgtg gaacgattga tacctgtttt tatgattgtg
     1681 tatcatttaa agtcttgcac tttacaattt atgtatctga tgaaacactt ggtacagttt
     1741 tacatagact gtcagaaaaa cagcgtagtg cgattttatt acgttatttt caaggaatga
     1801 acgacagaga aatttcagaa ctgtaccatg tatcacgttc tgctatttgc agtagaagaa
     1861 ctagaggact aaggaagtta aaaatgcttt tgagcgaaag gaagtaaaga aatgagtaaa
     1921 gattatggct atccctcttt tgaattgatt tgcaaagcgt caagcggaga tgaaatggca
     1981 gttaaagaga ttttgaagtt ttatgacggt tatatttcta aattatgctt gcgtcctttt
     2041 taccactctg aaagtggtaa aatcattatg caggttgatg aagaactaaa aggagaaatt
     2101 catacagaca tgatgaaagc aatattgaaa tttgaaatcg gagtgaagta atcaactgta
     2161 taaaaaaatc gggagataca cagtttcgtg ctatctccct tagtagtttt acggtcatat
     2221 caaggctcat tcaatcgtgg taaattcgtg gtaagatgag tgtttttcta tacttcaaaa
     2281 tgcttgaatg tatagtgttt atgcggataa ttgaa
//
LOCUS       DASJST010000429         1866 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.265, whole genome shotgun sequence.
ACCESSION   DASJST010000429 DASJST010000000
VERSION     DASJST010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.265"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            49..939
                     /locus_tag="U4B05_003168"
     CDS             49..939
                     /locus_tag="U4B05_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN2052219.1"
                     /translation="MLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLENSGTSE
                     NTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMS
                     KEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGL
                     SLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHS
                     AIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGN
                     NIDGVIVTVFPGSTHTNSRR"
     gene            1092..1577
                     /gene="vanZ"
                     /locus_tag="U4B05_003169"
     CDS             1092..1577
                     /gene="vanZ"
                     /locus_tag="U4B05_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN2052220.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          656 a          266 c          352 g          592 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc tttttttatt gttattgtta
       61 ttcttaatat acttaggtta tgactacgtt aatgaagcac tgttttctca ggaaaaagtc
      121 gaatttcaaa attatgatca aaatcccaaa gaacatttag aaaatagtgg gacttctgaa
      181 aatacccaag agaaaacaat tacagaagaa caggtttatc aaggaaatct gctattaatc
      241 aatagtaaat atcctgttcg ccaagaaagt gtgaagtcag atatcgtgaa tttatctaaa
      301 catgacgaat taataaatgg atacgggttg cttgatagta atatttatat gtcaaaagaa
      361 atagcacaaa aattttcaga gatggtcaat gatgctgtaa agggtggcgt tagtcatttt
      421 attattaata gtggctatcg agactttgat gagcaaagtg tgctttacca agaaatgggg
      481 gctgagtatg ccttaccagc aggttatagt gagcataatt caggtttatc actagatgta
      541 ggatcaagct tgacgaaaat ggaacgagcc cctgaaggaa agtggataga agaaaatgct
      601 tggaaatacg ggttcatttt acgttatcca gaggacaaaa cagagttaac aggaattcaa
      661 tatgaaccat ggcatattcg ctatgttggt ttaccacata gtgcgattat gaaagaaaag
      721 aatttcgttc tcgaggaata tatggattac ctaaaagaag aaaaaaccat ttctgttagt
      781 gtaaatgggg aaaaatatga gatcttttat tatcctgtta ctaaaaatac caccattcat
      841 gtgccgacta atcttcgtta tgagatatca ggaaacaata tagacggtgt aattgtgaca
      901 gtgtttcccg gatcaacaca tactaattca aggaggtaag gatggcggaa tgaaaccaac
      961 gaaattaatg aacagcatta ttgtactagc acttttgggg taacgttagc tttttaattt
     1021 aaaacccacg ttaactagga cattgctata ctaatgatac aacttaaaca aaagaattag
     1081 aggaaattat attgggaaaa atattatcta gaggattgct agctttatat ttagtgacac
     1141 taatctggtt agtgttattc aaattacaat acaatatttt atcagtattt aattatcatc
     1201 aaagaagtct taacttgact ccatttactg ctactgggaa tttcagagag atgatagata
     1261 atgttataat ctttattcca tttggcttgc ttttgaatgt caattttaaa gaaatcggat
     1321 ttttacctaa gtttgctttt gtactggttt taagtcttac ttttgaaata attcaattta
     1381 tcttcgctat tggagcgaca gacataacag atgtaattac aaatactgtt ggaggctttc
     1441 ttggactgaa attatatggt ttaagcaata agcatatgaa tcaaaaaaaa ttagacagag
     1501 ttattatttt tgtaggtata cttttgctcg tattattgct cgtttaccgt acccatttaa
     1561 gaataaatta cgtgtaagat gtctaaatca agcaatctga tctttcatac acataaagat
     1621 attgaatgaa ttggattaga tggaaaacgg gatgtgggga aactcgcccg taggtgtgaa
     1681 gtgaggggaa aaccggtgat aaagtaaaaa gcttacctaa cactatagta acaaagaaag
     1741 cccaattatc aattttagtg ctgaggaatt ggtctcttta ataaatttcc ttaacgttgt
     1801 aaatccgcat tttcctgacg gtacccccaa actaagattt catagataga ttttctgata
     1861 acttta
//
LOCUS       DASJST010000430          994 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.328, whole genome shotgun sequence.
ACCESSION   DASJST010000430 DASJST010000000
VERSION     DASJST010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.328"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            381..929
                     /gene="aac(6')"
                     /locus_tag="U4B05_003170"
     CDS             381..929
                     /gene="aac(6')"
                     /locus_tag="U4B05_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2052221.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          353 a          144 c          195 g          302 t
ORIGIN      
        1 acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg
       61 cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta
      121 tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa
      181 acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt
      241 tctgtataat tttttatttt ataagtaaga taaataatga aagaatagag atactgattg
      301 gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc
      361 aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga
      421 aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg
      481 cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag
      541 atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc
      601 atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt
      661 acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt
      721 tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg
      781 cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa
      841 tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa
      901 cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct
      961 tcaagaaata agaaggaatt tacgaaaatt actt
//
LOCUS       DASJST010000431          687 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2069
            SAMN38756195-rid20574593.guided.353, whole genome shotgun sequence.
ACCESSION   DASJST010000431 DASJST010000000
VERSION     DASJST010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756195
            Sequence Read Archive: SRR27167984
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028148.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:58:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 33 of 60
            Pseudo Genes (incomplete)         :: 24 of 60
            Pseudo Genes (internal stop)      :: 12 of 60
            Pseudo Genes (multiple problems)  :: 8 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756195-rid20574593.guided.353"
                     /isolate="VE2069"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-02-15"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>687
                     /locus_tag="U4B05_003171"
     CDS             <1..>687
                     /locus_tag="U4B05_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2052222.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPN"
BASE COUNT          289 a           62 c          113 g          223 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatat
//
